LOCUS       DASTXO010000001         2266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_294746, whole genome shotgun sequence.
ACCESSION   DASTXO010000001 DASTXO010000000
VERSION     DASTXO010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294746"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VFU96_00005"
     CDS             <1..519
                     /locus_tag="VFU96_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU5111664.1"
                     /translation="IAIALSSAETGNTAALGVALVLVATICYGFATNIVTPLQQRYGS
                     IPVMARMLWLATAFLLPYGLFGVSRSTFAWGSLAAMMALGFIGTGLAYIIMGGLAGSV
                     GPTRASFITYLIPVVALVLGVVFRDEIVAPVAIVGSLLVILGAILASRRETPAVTVAT
                     ATVHDIEDDDAA"
     gene            complement(524..1429)
                     /locus_tag="VFU96_00010"
     CDS             complement(524..1429)
                     /locus_tag="VFU96_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111665.1"
                     /translation="MKFTVEAWDPDYGAPSEADLVDSTENVDPSVEVLPEQWRPMPPD
                     GQPAPDILFVDGVRRVDANLWIDRDDRFPGFGLAATFAAGAVRCNSMARVVAAEVRRG
                     VFTGADADSVETSVGTYEIMSTKGSTPEELWLGIQERMGDLEGVVAEAQADVALVVVD
                     GPLSHRRHVEGAVGYVKTQHVQYLPEELRSILGTLPVGCRTPLFLTTTSWSRFSWYLR
                     LANGTGPAGGLVRCEVKADMTVAEAAGRANMVSASLPRYASARHKDPRAPQNLYPIGG
                     LERELRRRLGDRDLAMRALRRAAAA"
     gene            complement(1476..>2266)
                     /locus_tag="VFU96_00015"
     CDS             complement(1476..>2266)
                     /locus_tag="VFU96_00015"
                     /inference="COORDINATES: protein motif:HMM:NF024947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HEU5111666.1"
                     /translation="DAWRRFVIWLGETTATRSQELATLEKAVDVAAEASTTAEADLRS
                     WLEQFGIASIESPDRDLALVEAAKRAEIEEMQKNIAHALELEGELAIESGHVRVASAL
                     GHHLKANNFEAWLLEEALEVLIEGANRLLDELSGGGYSLRVRKSQFEVIDHRNAQLTR
                     TTRTLSGGELFLVALSLALTMAEQLAELTGMSSRLESVFLDEGFGSLDQESLDVVASV
                     LDELVGRGRTVGIVTHVRELAERMPVRFEITKGPETSSITRVGD"
BASE COUNT          386 a          773 c          698 g          409 t
ORIGIN      
        1 atcgccatcg ccctctcgtc ggccgagacg gggaacactg cggcgctcgg agtcgccctg
       61 gtgctggtgg caacgatttg ctatgggttc gccaccaaca tcgtcacccc gctccaacag
      121 cgatatgggt ccatccccgt catggccagg atgctgtggc ttgcgactgc gttcttgctc
      181 ccttacgggc tgtttggagt ctcccgatcg accttcgcct gggggtcgct ggcggcgatg
      241 atggcgctcg gcttcatcgg gaccgggctc gcctacatca tcatgggggg cctggcaggg
      301 agcgtcgggc caacccgagc cagcttcatc acatatctga tcccggtggt cgctctcgtc
      361 ctcggtgtcg tgttccgtga cgagatcgtg gccccggttg ccatcgtcgg gtcgctgctc
      421 gtcattttgg gagccatcct cgcctcgcgg cgggagacac cggccgtgac tgtggccacg
      481 gcgaccgtcc acgacatcga ggacgacgac gccgcctgaa ggctcaggcg gcagccgctc
      541 gcctcaaggc cctcatcgcc agatcacggt caccgagtct ccgacgaagc tcgcgttcca
      601 ggccaccaat cgggtacagg ttctggggtg cccgagggtc cttgtggcgg gcggacgcgt
      661 atcggggcaa cgaagcactc accatgttgg ccctccccgc cgcctcggcc acggtcatgt
      721 ccgccttgac ctcacagcga accagaccgc cggcaggccc ggtcccattg gcgagtctca
      781 ggtaccagga gaacctgctc cacgacgtcg tggtgaggaa gagcggagtc cggcatccaa
      841 ccggcagggt cccgaggatg ctcctcagct cttcggggag gtactggaca tgctgggtct
      901 tgacatagcc gaccgcccct tcgacatgcc ggcgatggga tagcggccca tcgacgacta
      961 cgagagcaac atcggcctgc gcctcggcaa cgaccccttc gagatcaccc atgcgctcct
     1021 gtatccccag ccacagctct tccggcgtgg atcccttcgt gctcattatc tcgtaagtcc
     1081 ccaccgaggt ctcgactgag tcggcgtcag ccccggtgaa gaccccgcgc cgcacctccg
     1141 cggccaccac ccgggccatg ctgttgcagc ggaccgcgcc ggcggcgaag gtcgcggcca
     1201 ggccgaaccc tgggaagcgg tcatcacggt cgatccagag attcgcatcg actcggcgca
     1261 ccccatcgac gaacaggatg tccggggcag gttggccatc ggggggcatc ggacgccatt
     1321 gctcggggag gacctcgacc gacggatcga cgttctctgt cgaatcgacg agatccgcct
     1381 cggagggagc cccgtaatca ggatcccagg cctcgaccgt gaacttcatc tcgcccccag
     1441 cagaggggag tgtggccgca gccgaagaag ccggctcaat ctccgaccct cgtgatcgaa
     1501 gatgtctctg gccccttggt gatctcgaac cggacaggca ttcgctccgc cagctcgcgt
     1561 acgtgggtga cgatgccaac cgtccggcca cggccgacca gttcgtcgag cacagacgcg
     1621 accacgtcga gagactcctg gtcgagtgag ccaaatccct cgtcgagaaa caccgactcg
     1681 agccgggatg acatcccggt cagctcggcc agctgctcgg ccatggtcag ggcgagcgac
     1741 agggcaacga ggaacagctc gccaccggag agggtgcgtg tggttctggt cagttgggcg
     1801 ttgcgatggt cgatgacttc gaactggctc tttctgactc tcagcgagta gccgccaccc
     1861 gatagctcgt cgaggagacg attggctccc tcgatcagca cctccaacgc ctcttcgagc
     1921 aaccaggcct cgaagttgtt cgccttcagg tggtgcccca gcgccgaagc gacacgcaca
     1981 tggccgcttt cgatcgccaa ctccccttcc agctcgagcg catgggcgat gtttttctgc
     2041 atctcctcga tctcggctcg cttggctgct tcgacgaggg ccagatcacg gtcaggtgac
     2101 tcgatcgagg cgatgccgaa ttgttccagc caggaacgaa ggtccgcctc cgccgtcgtc
     2161 gacgcttcgg ccgccacatc gacagccttt tcgagcgtgg ccaactcctg tgatcgggtc
     2221 gcggtcgtct ctcccagcca gatcacgaac cgacgccagg cgtcga
//
LOCUS       DASTXO010000002         2396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1547517, whole genome shotgun sequence.
ACCESSION   DASTXO010000002 DASTXO010000000
VERSION     DASTXO010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547517"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..849)
                     /locus_tag="VFU96_00020"
     CDS             complement(181..849)
                     /locus_tag="VFU96_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011206947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5111667.1"
                     /translation="MSIGVLLVDDQTLVRAGFAQLLGREEDIAVVGEAENGLEGLDLA
                     RELQPDVVLMDIRMPVMDGLEATRRIVLDPRTPDTKVLVLTTFELDEYVFEALRIGAS
                     GFLLKDTEPEELRRAVRVIASGDALLSPAVTRRLIAEYASKLARPPLDPTPLDVLTKR
                     EREVLALVAEGLSNQEIADRLYMSPATAKTHVSRAMTKLGARDRAQMVVIGYETGLVL
                     PGRE"
     gene            complement(846..1994)
                     /locus_tag="VFU96_00025"
     CDS             complement(846..1994)
                     /locus_tag="VFU96_00025"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU5111668.1"
                     /translation="MTARASGGPWWKRALVIDIVVVMVLAAVLVVLVSVASESGARQV
                     DSLAYVVVLAQALIQLFRRSRPVPVLVMSVILVFAYHAMGYPAIGNFPLVVPLFTVAV
                     TGHTTAAVVATAISTMGTLGWMLAGESQSFLLSFSIVVREAAVLIAVVMAGIAIRNRR
                     LLTIESRERLRLARVERDALAASERMRIARELHDIVAHTVAVIGIQARVATDTLADDP
                     DQARKALEVISTSTREATSELRATIDVLREGEDAPLTPAPGLSQLPALVDSVRSGGLP
                     VELVLTGDVRKLPGSVELTAYRVVQESLTNVVRHASASRAGVGIDYLPGLLTVTVTDD
                     GIGGEAGSGFGIRGMRERVVAAGGTLAADSTGNGGFRVRAEIPLHTDS"
     gene            complement(2070..>2396)
                     /locus_tag="VFU96_00030"
     CDS             complement(2070..>2396)
                     /locus_tag="VFU96_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU5111669.1"
                     /translation="IYALDPGPQPEAEVADPIEGRTIFLTKGCTGCHSREGDSETGFV
                     GPDLTGLADRAGNRVEGLSAEEYVRQSVLDPQAYIVDGYGSQMPVLPVDAEELDALIG
                     YLLSES"
BASE COUNT          428 a          806 c          763 g          399 t
ORIGIN      
        1 gtggcgatga cgtatcggtg ccggggacgc aggcagcgag gaggacggtg agtgccagtg
       61 ctgacagtgc ggtgatttgc ttgtgtctca tccggccaac gtatgagttg aacggcgtga
      121 ggtcgtcggc ccggcggaga cattggtctg cgaccgggtg agtaccggga ctgcctgggt
      181 ctactcccgc cctgggagga cgagcccggt ctcgtaaccg atgaccacca tctgggctcg
      241 atccctggct ccgagcttgg tcatcgcccg agagacatgg gtcttggccg tggccgggct
      301 catgtacagc ctgtcggcga tctcctgatt ggagagaccc tctgcgacca gcgccaggac
      361 ttcgcgttcc cgtttggtga gcacatcgag cggggtcggg tcgagtggcg gtcgggccag
      421 cttcgatgcg tattcggcga tcaaacgccg agtcaccgct ggggaaagca gtgcgtcacc
      481 ggaagcgatg acacgaaccg ctcggcgcag ctcctcgggc tcggtgtcct tgagcaggaa
      541 tccgctggcg ccgatgcgta gagcctcgaa cacgtactcg tccagctcga acgtggtgag
      601 cacgagcacc ttggtgtccg gcgtccgggg gtccaagacg atgcgccgtg tcgcctcgag
      661 gccgtccatg accggcattc ggatgtccat caacaccaca tcgggctgaa gctcacgggc
      721 caggtcgagg ccttcgaggc cgttctccgc ctcaccgaca accgcgatgt cctcctcacg
      781 gccgagcagc tgcgcgaaac ctgccctgac cagggtttgg tcgtcgacga ggaggacgcc
      841 gatgctcatg aatccgtgtg cagagggatc tcggcgcgga cccggaaacc gccgttacca
      901 gtcgagtcgg ccgccagtgt gccccctgcg gccacgaccc gctcccgcat ccctctgatt
      961 ccaaatccgg acccggcctc gcccccaatt ccatcgtcgg tcacggtcac tgtcaggagc
     1021 ccgggcagat agtcgattcc caccccggcc ctcgatgcac tggcatgtcg aacgacattt
     1081 gtcagcgact cctgcaccac gcggtaggca gtcaactcca ccgatcccgg gagcttgcgc
     1141 acgtcgccag tgagaaccaa ctcgaccggc aggcccccac tccgcaccga atcgaccagg
     1201 gcgggcaact ggctgaggcc aggagcggga gtcaatggag cgtcctcccc ctcgcgaaga
     1261 acgtcgatcg tggccctcaa ctccgacgtg gcctctcggg tggaagtgct gatcacctcc
     1321 agtgccttgc gcgcctggtc cgggtcgtcg gcgagcgtgt ccgtggccac ccgggcttgg
     1381 atgccgatca ccgcgacggt gtgagccacg atgtcgtgca attcacgtgc gatgcgcatc
     1441 cgctctgatg cagccagggc gtctcgctcg acgcgtgcca gccgaagccg ctcgcgggat
     1501 tcgatcgtga ggagtcgacg attgcggatg gcgatgccgg ccataaccac tgcgatgagg
     1561 accgctgctt cccgcaccac gatgctgaag ctgagcagaa atgattgtga ctctccggcc
     1621 agcatccagc ccagggttcc catggtgctg atcgcggtgg ccaccacggc ggcggtcgtg
     1681 tgcccggtca ctgccaccgt gaacaacggc accaccaacg gaaaattgcc aatggctggg
     1741 tagcccatgg cgtgataggc gaacaccagg atcaccgaca tcaccaacac cggtaccggc
     1801 cggctccgtc gaaacagctg gatcagggcc tgcgcgagaa cgacgacgta ggcgagactg
     1861 tcgacctgcc gggcgcccga ctccgaggcg actgacacca gaacgaccag caccgccgcc
     1921 aacaccatca cgacgacgat gtcgatcacc agggctcgct tccaccacgg cccgcccgag
     1981 gcgcgcgctg tcatgggcca accgtatagg gggatcgccc ctcagtcatc cgccggggga
     2041 ggtagcgatc gctacgaccc cgggggtagt cagctctcgc tgagcaagta accgatcagg
     2101 gcgtccaact cctcggcgtc gacgggcagc actggcatct gtgacccata cccgtcgacg
     2161 atgtaggcct gcggatcgag caccgactgt ctcacgtact cctcggcgga gaggccctcc
     2221 acccgattgc ctgcccggtc ggcgagaccg gtgaggtcgg gcccgacgaa cccggtctcg
     2281 gagtcacctt cccggctgtg acaccccgtg caacccttcg tgagaaagat cgtgcggcct
     2341 tcgatcgggt ccgccacctc cgcctccggt tgcggccccg gatccaaggc gtagat
//
LOCUS       DASTXO010000003         9849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1731693, whole genome shotgun sequence.
ACCESSION   DASTXO010000003 DASTXO010000000
VERSION     DASTXO010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 9849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9849
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1731693"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VFU96_00035"
     CDS             complement(<1..248)
                     /locus_tag="VFU96_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU5111670.1"
                     /translation="MDRIAVIGAGSWGTTAAALAAGGTPTILWARHQEVADRVNEDHR
                     NPRYLPGHSLPEGLVATSSLTEAIDGAGAVVMAVPSHG"
     gene            301..1383
                     /gene="ychF"
                     /locus_tag="VFU96_00040"
     CDS             301..1383
                     /gene="ychF"
                     /locus_tag="VFU96_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019434846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEU5111671.1"
                     /translation="MALSIGIVGLPNVGKSTLFNALTDADVLAANYPFATIEPNTGVV
                     PIPDPRLEVLARIFDSARVIPAAVTFVDIAGLVKGASEGEGLGNQFLANIRDANAICH
                     VVRGFTSQSVAHVDNELDASRDIETIETELAIADLQTIDKRLPRLEKESTIDRSLLPR
                     VDLLRDLRSVVASGRVVSRVPAIMKRASEFADLHLLTAKPVIYVFNLDEGGLRDEGLR
                     DRLAALVAPAESLFVDAQIEAEMGELDEVDRGELLASVGQDEPGLNAVIQAAYRTLGL
                     QSFLTAGEKEARAWTIRSGATAPEAAGAIHTDFQRGFIAAEVVDYEMLVEAGSWVTAK
                     TKGLVRTEGKAYVMRPNDVVEFRFNV"
     regulatory      1466..1557
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            1611..4436
                     /gene="gcvP"
                     /locus_tag="VFU96_00045"
     CDS             1611..4436
                     /gene="gcvP"
                     /locus_tag="VFU96_00045"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEU5111672.1"
                     /translation="MPNPNERFVDRHIGPGPDDIDKMLATIGAATLDDLVAETIPGSI
                     VDTDLDLPPALSEGQTIEKLRELASKNLVMTSLIGLGYHDTITPPVIQRNVLENPAWY
                     TAYTPYQPEISQGRLEALLNFQTMVADLTGLDVANASLLDEATAAAEAMAMCRRLAKS
                     DRSVFYVDDDCHPPTIEVVRTRASAAGVEVRMLSDGFEPDVAFGLLLQYPGSSGEIRD
                     HRSLVEEARGSGALVAVAADLLALTLLTPPGEWGADIAVGSAQRFGVPLGYGGPHPGF
                     IATKNEHVRGLPGRLVGVSHDTSGRIAYRLALQTREQHIRREKATSNVCTAQVLLAVI
                     AGFYSCWHGQAGLKEIAKETHRLTSLTAESLRAAGYEVVNASWFDTITVGTPGRASEL
                     IDRAIDRRINLGLVDDDHVRIAFDETTTDAIVVTLLEAFGAAVAGAPQTDSIPIDLVR
                     RSEFMTHPVFSLHRSEHSMLRYLRRLSDRDLALDRSMIPLGSCTMKLNATTEMIPITW
                     PEFSSIHPYSPQDQVAGYLELIDDLAGYLCRITGYDAVSLQPNAGSQGELAGLLAIRA
                     YLGSIGEGDRNICLIPSSAHGTNAASAVMAGMKVVVVATDVSGNVDLADLEAKAEAHS
                     EHLAAAMITYPSTHGVFEEGVRRLIEIVHRHNGQVYVDGANLNAMVGLAKPADFGADV
                     GHLNLHKTFTIPHGGGGPGVGPVGVKAHLAPFLPNHPLNQRAGPETGVGPISGAPFGS
                     AGILPIPYAYVRLMGAEGLRAASKVAILSANYLARRLDPYFPVLYAGNSGRVAHECIV
                     DVRPVTRSTGVTAEDVAKRLADYGFHAPTLSFPVAGTLMIEPTESESLEELDRFVEAM
                     IAIHGEVARVESGEWSAEDNPLHNAPHSAEQVTADEWSHVYSRETAAFPLASLRVDKY
                     WPPVGRIDGPGGDRHLVCACPPLEAYA"
     gene            4519..5952
                     /locus_tag="VFU96_00050"
     CDS             4519..5952
                     /locus_tag="VFU96_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111673.1"
                     /translation="MKFTGQVRVPEVDHPGVPATIVIEEGQTEVFLEGESLGRWSLYD
                     VRAARLVSSAFSLDLDGEEITFIADEPVDFAYKGVDHMAGVWARYKSMTIPRRVVAVG
                     RSRRGTLPSRISELREAMLSNLQTAGRPTARFAAFEAPPVPVEPAPWTKRLDAYSNVP
                     EPIDTDPIDDGPLALAPTVIDPISEPEIEEALAEIETDDTAEPETRSGLEPTSDEVAL
                     GIEPVLDEADGTVETDETAETDDVPALAAEIEPNSAPAVASIEVETSRAQLRPGPGLI
                     PRRVQRPRDEEEMEPSISPFWDEAWAEPPIEDEPYDDVPPDADLVLIDNEPPVAAAAV
                     VEVGEDVDLAADQVEPIVAEETAAAPAAPAPWEATHDDALEEPVSVDEWAVAPQVEEA
                     IRPEVVVDLDHLHVEPTSVESDLELLFAGAPTPEGEKSGLLGAVRSAFARNKTTHEHE
                     FVEAPGGIGIVRQICADCGYISIGVSD"
     gene            6012..7055
                     /locus_tag="VFU96_00055"
     CDS             6012..7055
                     /locus_tag="VFU96_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEU5111674.1"
                     /translation="MNPTLRPVILSGGVGTRLWPLSTKDTPKQFLELLGEPLFEATLA
                     RAMKLGGSEAIIVTGSEQIPAVEKSLADMEIDDATIVVEPSGRNTAPAVVAAALVADP
                     LDVLVVLPSDHLMTDEVAFRQAVIRAVDVARDGALVTFGIEPSRPETGYGYIEIGEPI
                     GPGFHIARFKEKPDEDEAAQLVTGGAHLWNSGMFVFGAAQILGEARRQAPGIVTAVEA
                     ALPTERSGTIRLDESFSGVPSISIDHAIMEGADNGVVIPMDAGWSDVGSWQSVWELSD
                     QDEHGNSLVGDVVAIDVADNYVRSETKAIAIAGVTGLVVIVTPDVVLIVPKEKSQMVR
                     DLAEGWEASRPAD"
     gene            7064..7369
                     /locus_tag="VFU96_00060"
     CDS             7064..7369
                     /locus_tag="VFU96_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU5111675.1"
                     /translation="MRQAAIVTLLGVLISACVGRPAPDAGGEEIYLQLCSNCHGDQLE
                     GGIGPPLGPGSNAAEQPDEYLRVTITEGRGRMPSFSSSLSEDQVMRLIDYLRESQTS"
     gene            7366..8325
                     /locus_tag="VFU96_00065"
     CDS             7366..8325
                     /locus_tag="VFU96_00065"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016663982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HEU5111676.1"
                     /translation="MSVVLEALELYPIRIPMRHRFRRVDHREAVLIRGPAGWGEFSPF
                     PDYPPHVTTRWLAAALEAACSILPEPGRPTIPVNVTVPAVAPEIAARLVLESGAGTAK
                     VKVGEPGHTMEEEEARLAAVRAAIGPEGKIRIDVNAAWDLETAATRLASYARFDLEYV
                     EQPVKSLEEMVELKQRIGVPLAADELVRLRPDPLEVASMGAADILVVKVQPLGGVART
                     LDIAARAGIPVVVSSALETSIGIYSGLLVASLLPDLPFACGLGTVSLIEGDPTRLPLV
                     SMNGELEVRRPEPDPELLERWRPDRDRSAELLRKVRNAAEVLT"
     gene            8342..>9849
                     /gene="menD"
                     /locus_tag="VFU96_00070"
     CDS             8342..>9849
                     /gene="menD"
                     /locus_tag="VFU96_00070"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HEU5111677.1"
                     /translation="MTQPNPSTAQARVIVDELHRHGVDYVVISPGSRSAALAIAFEEH
                     SGIATRVVLDERSASFHALGRSRATGAPVVALATSGTALANYLPAVIEADLSLIPLIL
                     ISADRPPELLNVGANQTIDQHGIFGEKVRWSCAIPAAEPDLDSNAYWRSTVSQTMARA
                     KGHGARPGPVHLNVAFREPTVPVSDDGRTRAEPYNHPIEGRAGEEPWQHHQQIRPVGA
                     NLDMGGRGLVVVGEGDFDQKEVFAAARKLDWPVLATATSGLRVFDVVTTYHHLLVTGV
                     PSVLRPDLVVTVGRIGPSDRLGSLTSLPVPQVNVDRWGAWNDPRRHSTHLLQADPAET
                     LAGFSPASDDGLLRNWLEANATMRSALDDHLSGDETPSGPGVAHALAPVDDVIMAAAS
                     SMPIRDVDAHLARTGRVIANRGASGIDGFVSTALGAASASTGAIAFAGDLSFLHDSSG
                     FIVDEIGDVVFVVVDNGGGGLFDLLPQAEHAPAFERLFVTPHHLDLSRVASA"
BASE COUNT         1685 a         3220 c         3221 g         1723 t
ORIGIN      
        1 aacccatgtg acgggacggc catcacgact gcgccggcac cgtcgatcgc ttcggtgaga
       61 ctggaagtgg cgacgaggcc ctcagggagc gaatgaccgg gcagataacg cgggtttcga
      121 tgatcctcgt tgactctgtc cgccacctcc tggtgccgtg cccaaaggat ggtgggggtg
      181 ccaccggcgg cgagggctgc tgcagtggtc ccccacgacc cagcgccgat cactgcgatc
      241 ctgtccatcg gcggcgacgc tagcgggggt gccgggggcc ctccctaacc tctggtcccg
      301 atggcgctat cgattggcat cgtcggtctc cccaacgtcg gcaagtcgac gctcttcaac
      361 gcgctgaccg acgccgacgt gcttgccgcc aactatccct ttgccacgat cgagccgaac
      421 accggggtgg tcccgatacc cgacccccgc ctggaggtcc tcgcccggat cttcgactcg
      481 gcacgggtga tcccggcagc ggtcactttc gtcgacatcg ctgggctggt gaagggcgcg
      541 agcgagggtg agggcttggg caaccagttc ctggccaaca tccgggatgc caatgcgatc
      601 tgccatgtcg tgcgtggctt cacgtctcag agcgtcgccc acgtcgacaa tgagctcgac
      661 gcatcgaggg acatcgagac catcgagacc gagcttgcga tcgccgacct gcagacgatc
      721 gataagcgac tgccgcgtct ggagaaggag tcgaccatcg accggtcgct gctgccccgc
      781 gtcgacctat tgagggacct ccggtcggtc gtggcctcgg ggcgagtcgt gagccgtgta
      841 ccggcgatca tgaagcgtgc gtctgagttc gccgatctcc atctgctcac cgccaaaccc
      901 gtgatatatg tcttcaatct cgatgagggc ggtctgcgcg acgaggggct ccgtgaccgg
      961 ctggccgcac tcgttgcccc ggccgagtcg ctttttgtcg acgcccagat cgaggccgag
     1021 atgggcgagc tggatgaggt ggatcgcggg gagctgctcg cctcggtggg acaggacgag
     1081 cccggcctga acgcagtgat ccaggcggcc taccgcacgc tcggactgca gagcttcctc
     1141 actgccggtg agaaggaggc ccgcgcctgg accatccgct ccggagccac cgcgccggag
     1201 gcggcggggg cgattcacac cgatttccaa cgcgggttca tcgcggctga agtggtggac
     1261 tacgaaatgc tggtcgaagc aggctcctgg gtgacggcaa agacgaaggg cctggtcagg
     1321 accgagggca aggcatacgt gatgcgtccc aacgacgtcg tcgagtttcg cttcaacgtc
     1381 tgatagcggc cgattggcgc gaggccttcg acgcgccgct ggccggcacg tacgagtcgg
     1441 ccatcaatta ggattagcga gccatcgacg ccgaatggga gagcccctgg ttggcagggg
     1501 cgccgaaggg gcaactcctc cccggaaact ctcaggcact tggaccgttc ggtcgaggca
     1561 tcgctgaagg caacgacagc gggggagggc attgcaccct gaggagagag atgcccaacc
     1621 ccaacgagcg gttcgtcgac aggcacatcg gtcccggacc ggacgacatc gacaagatgc
     1681 tggccacgat cggcgcggca accctcgacg atctggtcgc agaaaccatc cccggctcga
     1741 tagtcgacac cgatctcgac cttccacctg ccctgtccga gggccagacg atcgagaagc
     1801 ttcgggagct ggcctccaag aacttggtca tgacctcctt gatcggtctc ggctatcacg
     1861 acaccatcac gccaccggtg attcagcgca acgtcctgga gaacccggcc tggtacacgg
     1921 cgtacacgcc gtatcaaccc gagatatcac aaggtcggct cgaggccctg ctcaatttcc
     1981 agacgatggt cgccgacctc accggtctcg acgtcgccaa cgcctcgctg ctcgacgagg
     2041 cgactgccgc ggcggaggcc atggccatgt gccggcgtct cgccaagagc gatcgatcgg
     2101 tgttctacgt cgacgacgac tgtcatcccc ccaccatcga agtggtgaga actcgggcct
     2161 cggcagcggg ggtcgaggtc cggatgctct cggatggttt cgagcccgac gttgcttttg
     2221 gcctccttct ccaataccca ggatcctcgg gcgagatcag ggatcaccga tcactggtcg
     2281 aggaggcgcg tgggtcggga gcactggtcg cagttgcagc cgatctgctc gcactgacgt
     2341 tgctcacgcc acccggggaa tggggtgccg atatcgcggt gggctcggcg cagcggttcg
     2401 gcgtgccgct cggatacggc gggcctcatc ccggattcat cgccaccaag aacgagcacg
     2461 tccggggtct gcccggccgc ctcgtcggag tgtcccatga cacgtcgggc aggatcgcat
     2521 accggctcgc cctccagacg cgggagcaac acattcgacg ggagaaggcc acctctaatg
     2581 tgtgcactgc ccaggttctc ctcgcggtca tcgccggctt ctattcctgt tggcatgggc
     2641 aggccggtct gaaggagatc gccaaggaga cccaccggct cacctcattg accgccgaat
     2701 cgctgcgtgc ggccggatac gaagtggtca acgcgtcctg gttcgacacg atcacggtcg
     2761 gcaccccggg ccgggcgtcg gagttgatcg atcgggccat cgaccgacgt atcaacctcg
     2821 gattggtgga cgatgatcac gtgcgcatcg ccttcgacga gacgacgacc gacgcgatcg
     2881 tcgtgacgtt gctcgaagcg ttcggcgcgg ccgttgccgg tgcgccccaa accgattcca
     2941 taccgatcga tctcgtcagg cgttcggagt tcatgaccca cccggtgttc agcctccacc
     3001 ggtcggagca ttcgatgttg cgctacctgc gacgcctgtc cgatcgcgac ctcgccctcg
     3061 accgaagcat gatcccgctc ggatcctgca cgatgaagct caacgcaacg acagagatga
     3121 tcccgatcac gtggcccgag ttctcctcga tccacccgta ctcgccgcag gatcaggtgg
     3181 cgggatacct ggaattgatc gacgacctcg ccggctatct gtgccggatc accggttatg
     3241 acgccgtgtc gctgcagccc aacgcgggct cgcaaggaga actggcaggg ctgctcgcca
     3301 tcagggcgta tctgggctcg attggcgagg gggatcggaa catctgtttg attccctcgt
     3361 cggcacacgg gaccaacgcc gcttcggcgg tcatggccgg gatgaaagtg gtcgtcgtgg
     3421 cgaccgatgt ctcggggaac gtcgatctgg ccgatctcga agccaaggca gaggcacact
     3481 ccgagcacct cgccgcggcg atgatcacct atccctcgac acacggtgtc ttcgaggagg
     3541 gcgtccggcg cctgatcgag atcgtgcacc ggcacaacgg ccaggtctat gtggacgggg
     3601 ccaatctgaa cgcgatggtg ggtctggcca aaccagcaga cttcggcgcg gacgtcggcc
     3661 atctgaacct ccacaagact ttcaccatcc cccacggcgg cgggggcccg ggtgtcggcc
     3721 cggtcggtgt caaggcgcat ctggcaccgt tcttgcccaa tcaccccttg aaccagagag
     3781 cggggccgga gacaggagtc gggcccatct cgggagcgcc attcgggtcg gctggcatcc
     3841 tccctatccc atatgcgtac gtccggctga tgggcgccga gggtctgagg gcagcgagca
     3901 aggtggccat cctctcggcg aactacctgg cccggcgtct cgacccctat ttcccggtgc
     3961 tctatgccgg gaacagcggg cgcgtcgccc acgagtgcat cgtcgacgtc cgtccggtga
     4021 cccgatccac aggggtcact gccgaagacg ttgccaagcg tctcgccgat tacgggttcc
     4081 atgcccccac gctctcattc ccggtggccg ggacgctgat gatcgagccg accgagtccg
     4141 agagcctcga ggagctggat cggttcgtag aagcgatgat cgccattcat ggcgaagtgg
     4201 cccgtgtcga gtcaggggag tggtccgccg aggacaaccc gctccacaac gcacctcatt
     4261 ctgccgagca ggtgacagcc gatgaatgga gtcatgtcta ctcccgcgag actgccgcct
     4321 tcccgctcgc ttcacttcgg gtcgacaagt actggccacc cgtgggtcgt atcgatggcc
     4381 ccggtggcga ccgccacctg gtctgtgcct gcccgcctct ggaggcctac gcctgactct
     4441 cggcttgaca ttcgccgcct ggatgggatc ttgatgcatt gcacaggcgg gtagggacgc
     4501 agaggtgacg gtgtaagggt gaaattcacc ggtcaggtga gggttccgga ggtcgaccat
     4561 cccggagtcc cagcaacgat cgtgatcgaa gagggccaga ccgaggtatt cctcgaaggg
     4621 gaatcgctcg gccgctggtc cttgtacgac gtgcgcgcgg cgcgtctcgt ctccagtgct
     4681 ttctctttgg atctcgacgg tgaggagatc accttcatcg ccgacgagcc tgtcgacttc
     4741 gcctacaagg gcgtcgatca catggcggga gtctgggctc gctacaagtc gatgaccatc
     4801 ccgcgtcgag tcgtcgcggt cggccgttcg cgacgcggca cccttccatc gcggatctca
     4861 gagctccgcg aggcgatgct gtcgaatctg cagaccgcgg gccggcccac cgcgcgtttc
     4921 gccgctttcg aggccccgcc tgtcccggtg gagccggcac catggaccaa gcgcctcgat
     4981 gcctactcca atgtgcccga gccaatcgac accgatccga tcgatgacgg tcccctcgct
     5041 ttggcgccca ccgtcatcga cccgatctca gagccagaga tcgaggaggc cctcgccgag
     5101 atcgaaacgg acgatacggc cgagcccgag acacggtcgg gcctggagcc gacctccgac
     5161 gaggtggcgc tcggtatcga gccagtgctc gatgaggcag atggcacggt cgagacggac
     5221 gagacggctg agacagatga tgtacccgcc ctcgccgcgg agatcgagcc gaattcggca
     5281 ccggcggtgg cgagcatcga ggtcgagacg tcccgtgctc aattgcgacc aggcccaggt
     5341 ctgatcccgc gccgggtgca gcggccccgg gatgaggaag agatggagcc ctccatctca
     5401 cccttctggg acgaggcgtg ggcagagccg ccgatcgagg acgagccata tgacgatgtg
     5461 cccccagacg ccgacctggt cctgatcgac aacgaaccgc cggtggcagc ggccgccgtt
     5521 gtcgaagttg gcgaagacgt ggacctcgcg gctgaccagg tcgagcccat tgtcgccgag
     5581 gagactgctg cggcgcccgc cgctcccgcc ccgtgggaag caacccatga cgatgctctc
     5641 gaggaaccgg tgtcggtcga tgagtgggct gtggccccgc aggtcgagga ggcaatccgg
     5701 cccgaggtgg tggtcgatct cgaccatctc cacgtggagc cgacatcggt cgagtccgac
     5761 ctcgagctcc tgttcgccgg cgcaccaaca cccgaaggtg agaagagcgg gctgctcggg
     5821 gcggttcgct cagctttcgc ccgcaacaag acgacacatg agcatgagtt cgtcgaagcg
     5881 cccggcggga tcggcatcgt ccggcagatc tgcgccgact gtggctacat ctccatcggg
     5941 gtctctgact gactcgattg ccggcaggcg atccaactga ggccgggcat cggcccgggt
     6001 accctcgccg ggtgaacccc acgctccggc ccgtgatcct ttccggcggg gtcggcacac
     6061 gtctctggcc tctttcgacc aaggacacgc cgaaacagtt cctcgagctt ctcggtgagc
     6121 cattgttcga ggcaacgctg gccagggcga tgaaactcgg cgggtccgag gcgataatcg
     6181 tgaccgggag cgagcagatc ccggctgtcg agaaatcgtt ggccgacatg gagatcgatg
     6241 acgccaccat cgtggtcgag ccatccggga ggaacactgc ccccgcggtg gtggcggcag
     6301 ccctggtcgc agaccccctg gacgtgctgg tcgtccttcc ctccgatcac ctcatgaccg
     6361 acgaggtcgc atttcgccag gctgtcatca gggcggtcga tgttgcccgc gacggagccc
     6421 tcgtcacatt cgggatcgag ccgagccgcc cggaaaccgg atatggatac atcgagatcg
     6481 gggagccaat cgggcctggg ttccacatcg cccggttcaa ggagaagcct gatgaggacg
     6541 aggcggcaca actggtcacc ggcggtgccc acctctggaa ttcggggatg ttcgtctttg
     6601 gagcggcaca gattctcggc gaggcgaggc gccaggctcc cgggatcgtc acggccgtgg
     6661 aggcggccct gccgacggag cgctcgggga cgatcagact cgatgagagc ttctccggag
     6721 tgccctcgat ctcgatcgac cacgccatca tggaaggggc ggacaacgga gtggtcatcc
     6781 cgatggacgc cggctggagc gatgtgggct cgtggcaatc ggtctgggag ctgagcgacc
     6841 aggacgaaca tggcaattcc ctcgtcggtg atgtggtggc gatcgacgtt gccgacaatt
     6901 acgtccgatc ggagacgaag gccatcgcca tcgccggggt gacgggcctc gtagtgattg
     6961 tcaccccgga tgtggtcttg atcgtcccca aagagaagtc ccagatggtg cgcgaccttg
     7021 cggagggctg ggaagcgagt cggcccgcag attgaggcac ccgatgagac aggcggcgat
     7081 cgtgaccctg ttgggggtgc tcatctcggc gtgtgtaggg cgcccggcgc ccgatgcagg
     7141 cggggaggag atctacctcc agctgtgctc gaattgccac ggtgatcagc tcgaaggcgg
     7201 gatcggcccg ccgctgggtc ctggctccaa cgccgccgaa cagccggacg agtatctccg
     7261 tgtgaccatc accgagggaa ggggccgcat gccgtcgttc tcctcgtccc tgagcgagga
     7321 ccaggtgatg cgcctgatcg actacttgag ggagtctcag acctcatgag tgtcgtgttg
     7381 gaggccctgg agctgtatcc gattcggata cccatgcgtc accgattccg ccgggtcgat
     7441 caccgcgagg ccgttctcat cagggggccg gccgggtggg gagagttctc accgtttccc
     7501 gactacccgc cacatgtcac gacccggtgg ctggccgcgg ccctggaggc cgcctgcagc
     7561 atccttcctg agccgggccg gcccacgatc ccggtcaatg tgacggtgcc ggctgtggcg
     7621 cccgaaatcg cggcccgact cgtcctggag tcgggtgcgg gcaccgccaa ggtcaaggtt
     7681 ggagagcccg ggcacacgat ggaggaggag gaagcccggc tcgccgcggt ccgggccgcg
     7741 atcgggcccg agggcaagat ccgcatcgac gtgaatgcgg cctgggacct cgagactgcc
     7801 gccacgcgtc ttgcctccta cgcccgattc gacctggagt acgtcgagca accggtcaag
     7861 agcctggagg agatggtcga gctgaaacag aggatcgggg tcccgctcgc cgccgatgaa
     7921 ttggtccgcc tccgtcccga cccgctggaa gtcgcctcga tgggcgccgc cgacatcctg
     7981 gtcgtgaagg ttcaaccatt gggcggtgtg gccaggaccc tggacatcgc ggcgcgagcc
     8041 ggcatccccg ttgtggtgtc atctgctttg gagacgtcga tcgggatcta ctccggtctc
     8101 ctcgtcgcct ctctccttcc cgaccttccg tttgcctgtg gtctcgggac ggtgtccctg
     8161 atcgaaggcg atccgacccg tctcccgctg gtatcgatga atggcgagct cgaagtgcgc
     8221 cgcccggagc ccgacccgga actgctcgaa cggtggcgac ccgatcggga caggtctgcc
     8281 gagctcctgc gtaaggtcag gaacgccgcc gaggtcctca cctgacccac cgagatcgga
     8341 catgacacag cccaacccaa gtaccgccca ggcccgggtc attgtcgatg agctgcaccg
     8401 acacggtgtc gactatgtgg tcatctctcc tggctcccga tcggccgccc tggcaatagc
     8461 tttcgaggag cattcgggta tcgcgactcg ggtcgtcctc gatgagcgct ccgcgtcgtt
     8521 tcatgccctg ggccgaagcc gggcgacggg agcaccggtg gtcgcactgg cgacatcggg
     8581 cacggcgctc gccaactacc tcccggccgt gatcgaggcc gacctgtcgc tcatccccct
     8641 gatcctgatc tccgccgatc ggccgcccga gcttctcaac gtcggcgcca accagacgat
     8701 cgaccaacat ggcattttcg gggagaaggt ccgttggtca tgtgccatcc ccgcggccga
     8761 gcccgatctc gactccaatg cctactggcg ttcgacggtg tctcagacca tggcgcgggc
     8821 caagggccac ggggcacgac ccggcccggt ccatctcaac gtcgcctttc gtgagcccac
     8881 ggtcccggtc tccgacgacg gccggaccag ggccgagccc tacaaccacc caatcgaagg
     8941 gcgggccggc gaagagccgt ggcagcatca ccagcagatc agacctgtgg gcgccaacct
     9001 cgacatgggg ggtcgcggtc tcgtggtggt cggagagggc gatttcgatc agaaggaggt
     9061 gttcgcggct gcgcggaagc tcgattggcc ggtgctcgcc accgccacct cggggctcag
     9121 agtgttcgat gtggtcacca cctatcacca tttgctggtg acgggggtgc cttcggtgct
     9181 ccggcccgac cttgtggtga ccgttgggcg gatcggtccc agcgaccgcc tcggatcgct
     9241 gacctcattg ccggtgcccc aggtgaacgt cgatcgctgg ggagcctgga acgacccgcg
     9301 ccggcattcc acccacctcc tgcaggccga tccggccgag accctcgccg gcttctcccc
     9361 ggcatcggat gacggtctcc tccgcaactg gctggaggcg aacgccacca tgaggtcggc
     9421 tctcgatgac catctctccg gcgacgagac gccctccggc ccgggggtgg cccatgccct
     9481 cgctcccgtc gatgacgtga tcatggccgc ggcatcgtcc atgccaatca gggacgtcga
     9541 tgcgcatttg gcccggacgg ggagggtgat cgccaatcgc ggtgcgtccg ggatcgacgg
     9601 ctttgtctcc actgcgttgg gcgccgcttc ggcttcgaca ggcgcgatcg ccttcgccgg
     9661 ggacctctcg tttctccatg actcttcggg cttcatcgtc gacgagatcg gcgacgtggt
     9721 gttcgtggtc gtcgacaacg gtggtggcgg cctgttcgat ctcctaccac aggccgagca
     9781 tgcccccgcc ttcgagcggc tgttcgttac cccacatcat ctcgacctga gccgagttgc
     9841 ctctgccta
//
LOCUS       DASTXO010000004         2005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_663388, whole genome shotgun sequence.
ACCESSION   DASTXO010000004 DASTXO010000000
VERSION     DASTXO010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663388"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1097)
                     /locus_tag="VFU96_00075"
     CDS             complement(54..1097)
                     /locus_tag="VFU96_00075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5111678.1"
                     /translation="MDLGLFIEFPCRQGMTDREAFAECFALVDAAEALGVGSVWLAEY
                     HFAPISVLSSPITVASAIAARTQRIRIGLAVVLLPLAHPIHIAEDIATLDHISLGRVE
                     FGVGRGTFPDTHDGFNSPFAESRERFEEYLEIIVKAWTQERFSFAGKYYRCDNLWVRP
                     KPLQRPHPPIRVGITSEVTFPLVGRLGFPIIINPSRVFALSELAPYIQQYREAWHAAG
                     HAGIPQVGLRVPVYVAKTAERAYEEPKVSTMAAVQNLGNRVADSASRVGTTGDWSLQA
                     ERVRRMSYEDWLRDKVVYGIPEAVVDRLQELREELDLTQILYEVNHGRQIPYALQLEN
                     LRLINEYVMPRLK"
     gene            complement(1106..1615)
                     /locus_tag="VFU96_00080"
     CDS             complement(1106..1615)
                     /locus_tag="VFU96_00080"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5111679.1"
                     /translation="MSAPKEIGHIVLNVTDVERSTRFYRDVVGFQVSRYRPDGTGAFL
                     TCGVVHHNLALFKAPAGARPPEKGAIGLNHFAFKVESYRALQAAHERLVEARATIDHI
                     VDHGMTRSVYFLDPDGIMMELFSDTYATEEEGLAYMKSTRGGAVPMDIAAAEPARAEI
                     PQADFTRIG"
BASE COUNT          372 a          621 c          647 g          365 t
ORIGIN      
        1 agggctagct acctgagcta cgagcgaaga aggatcgccg ctgcggagga ggactatttg
       61 agccgcggca ttacgtactc gttgatcagc cgtagatttt ccaattgcag cgcgtaagga
      121 atctggcgtc cgtggttgac ctcatacaga atctgcgtca ggtcgagctc ctcgcgtagc
      181 tcttggagtc ggtcgaccac ggcctcgggt attccgtaga ccaccttgtc cctcagccag
      241 tcctcgtaac tcatgcgtcg tacgcgttcg gcctgaaggc tccagtcacc ggtcgtgccc
      301 actcgggaag cgctgtccgc cacgcgattg cccaagttct gcacggccgc catggtgctc
      361 accttcggct cctcgtaggc ccgctcggcc gttttggcca cgtagacggg caccctgagc
      421 ccgacctggg gaatgcccgc atgacctgcc gcgtgccatg cctcgcggta ctgctggatg
      481 tacggcgcca gctcggagag agcgaacacc ctggaggggt tgatgatgat gggaaacccc
      541 aggcgtccga cgagcggaaa ggtgacttcc gaggtgatac cgacgcggat gggcgggtga
      601 ggccgctgca gcggtttcgg tcggacccag aggttgtcgc accgatagta cttgcctgcg
      661 aaggagaagc gctcctgggt ccacgccttg acgatgatct ccaggtactc ctcgaaccgc
      721 tctcggctct cggcgaaggg cgagttgaag ccatcgtgcg tgtcgggaaa cgtgccccgc
      781 cccacgccga actcgacccg gcccagactg atgtggtcca gcgtggcgat gtcctcggcg
      841 atgtggatcg ggtgggccag gggtaagagc accaccgcca ggccgatgcg gatgcgctgt
      901 gttcgggcgg cgatggcgct ggcgaccgtg atgggggagg agaggacact gatcggcgca
      961 aaatggtact ccgccagcca tacggagccg acaccgaggg cctcggctgc atcgaccagc
     1021 gcaaagcatt cggcaaaggc ctcgcggtcc gtcatcccct ggcggcaagg gaactcgatg
     1081 aagagcccga ggtccatgag accagttagc cgattcgagt gaagtcggcc tgggggatct
     1141 cagctcgagc gggttccgcc gccgcgatgt ccatcgggac agcccctcca cgggtggatt
     1201 tcatgtaggc cagtccttcc tcctcggtgg catacgtatc cgagaagagc tccatcatga
     1261 tcccgtccgg atcaaggaag taaacgcttc gggtcatgcc gtggtccacg atgtggtcga
     1321 tggtagcccg agcctccacc aaccgctcgt gggcggcctg cagtgcccgg tagctttcca
     1381 ccttgaacgc aaagtgattc aggccgatcg ctcccttctc tggcggccgg gcccccgccg
     1441 gcgctttgaa cagggcgagg ttgtgatgca cgacgccgca ggtcaaaaag gcgcccgttc
     1501 cgtcggggcg ataccgagac acctggaacc cgaccacatc ccgatagaat ctggtggacc
     1561 gttcgacgtc ggtgacgttg aggacgatat gcccgatctc tttcggtgcg ctcattctga
     1621 tttcctcctg ttccggccgc cgtgcgctcg cgattcgggc actctagctg catcgaagga
     1681 gagatggcaa ggcagacgtg cagtgtggca gcggtcagct cgtcaccgag aagcacgacc
     1741 gagacagtgt gatgatggac gccggccggc cgtactcttt cgatcgccac ggcgaaagag
     1801 tacactgccg gcaacccaag gaggtagcgg ccatgatcga catgcatgct cactggagac
     1861 ccgccgagct cgccgacgcc ctgcgtggcc ggacccggga accgcgcatc ctgcgcaacc
     1921 aggacggagt cgaggtgctg aagtcgccgc ggatgagcga agagtcgctc gtcaacgcct
     1981 tcgatcacgt cgatttccat ctcga
//
LOCUS       DASTXO010000005         3058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_295065, whole genome shotgun sequence.
ACCESSION   DASTXO010000005 DASTXO010000000
VERSION     DASTXO010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295065"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VFU96_00085"
     CDS             <1..525
                     /locus_tag="VFU96_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU5111680.1"
                     /translation="PALNGKRYLVPGQITLLRLQADEPGTYWGHCAEFCGLSHSLMRA
                     RVQAVEPAEYEAWLEEQQTAAATPGEGDIGFEGFQLFAEKGCTQCHTVRLEDEVLGPT
                     DRASFNGPELTHFASRPVFAGAVLPAEGQTRDEALKDWLADPPYIKPGSFMPDLALTE
                     AEIDALIVWLDTLE"
     gene            522..2609
                     /locus_tag="VFU96_00090"
     CDS             522..2609
                     /locus_tag="VFU96_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEU5111681.1"
                     /translation="MTATAERPTTTIFRRPSSTTGLWSWLTTVDHKRIGIMYGYAAFT
                     FFIIGGVEALMLRIQLSQANGEFLTAGAYNALFTMHGTTMIFLFVMPASAALMNYMLP
                     LLIGARDVAFPRLNALSWWIFFFGGIFLYSTFIFGTSAPPIGVDGLGGIDGNLAPGGG
                     PGSFNFLADSPDGGWFGYQPNAGPLYSPGTAMDYWALGLQIIGLASLVSSVNFLVTVL
                     NLRAKGMRLLRMPVFAWMTTITSFLLLFSLPIIAVALFMVTFDRQFGSLFFAAQAGGD
                     VILWQHLFWLFGHPEVYILILPAMGIVSEILPTFSRKPLFGYSAVVFAGAAIAFLGFG
                     VWAHHMFASGITPVAQAAFGLATMTIAIPTGIKIFNWLGTMWMGRIRLATPMLFAIGF
                     VAMFTIGGLSGVTHAVVPSDWQQTDTYYIVAHFHYVIFGGAVFGLFGGLYYWFPKLTG
                     RVMNERLGKIHFWLMLLGFNLTFGPMHWLGLQGMVRRTWKYAPETGLEPWNVVVTIGA
                     FIIALSILIFMFNWFTSRRRGEASGLDPWDARTVEWMSPNPTPEYNFAVPPVVTSLDH
                     FWHVKYEEDAEGRAVRKEDGDEIVATIEHEQLNPEAPIHLPNPSYFPFFFALGLPLMF
                     YGVIYHEAMWGKGLILVGLILSLSAVIGWAIEPLEEPHDEHEVPAEHDEGDHGAGELE
                     PAVEAEASESIDD"
     gene            2602..>3058
                     /locus_tag="VFU96_00095"
     CDS             2602..>3058
                     /locus_tag="VFU96_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEU5111682.1"
                     /translation="MTELTHEADLDQAHPEHEGTGVEDRKLGMWLFLSSEFLFFGALI
                     SNYLLYSHRPGFEGIYPAEIYDIPFTSVSSFVLLMSSLTMVLSHNALSRGDQNGTRTW
                     LFATAALGAVFLGGQVFEFTEFITAYGMKMSSNPAASAFYLLTGFHGTHV"
BASE COUNT          486 a         1046 c          903 g          623 t
ORIGIN      
        1 cccgccctga acggcaagcg ctacctggtc ccgggtcaga tcaccctgct ccgtctccag
       61 gccgatgagc ccggcaccta ttggggtcat tgcgccgagt tttgcggtct ctcccattcc
      121 ctgatgcggg ccagggtcca ggcagtcgag ccggcggagt acgaggcatg gctcgaggag
      181 cagcaaaccg cggccgccac acccggggaa ggggacatcg gattcgaagg gttccagctg
      241 ttcgcagaga aagggtgcac ccagtgccac accgtccgtc tcgaggatga ggttctcggg
      301 cccacggacc gcgcctcgtt caatgggcct gagctcactc acttcgcctc acggccggtc
      361 ttcgccgggg cagtcctccc cgccgaggga caaactcggg acgaggcgct caaggactgg
      421 ctcgccgacc cgccgtacat caagcccggg agcttcatgc ccgacctcgc cttgaccgag
      481 gctgagatcg atgctctcat cgtctggctg gataccctgg aatgaccgcc accgccgaac
      541 gtcccaccac cacgatcttc cgccgtccca gctcgacgac gggtctctgg tcctggctca
      601 cgaccgtgga ccacaagcgg attgggatca tgtacggcta cgccgccttc accttcttca
      661 tcatcggcgg tgtcgaggcg ctcatgctcc gcatccagct ctcccaagcc aacggtgagt
      721 tcctgaccgc gggtgcctac aacgcgctgt tcacgatgca cgggacgacc atgatcttcc
      781 tgttcgtcat gccggcatcg gctgcgctga tgaactacat gctcccgctg ctgatcgggg
      841 cgagagacgt ggccttccct cggctcaacg cgctttcgtg gtggatcttc ttctttgggg
      901 ggatcttcct ctactcgacc ttcatcttcg ggacctcggc gccaccgatc ggagtcgacg
      961 gcctaggggg catcgatggg aacctggccc ccggaggtgg gcccggaagc ttcaacttcc
     1021 tggccgactc gccggacggt ggttggttcg ggtatcagcc caatgccggg cccctctact
     1081 ccccgggcac ggcgatggac tactgggccc tcggcctcca gatcatcggc ctcgcctcac
     1141 tggtgtcctc ggtcaacttc ctggtcacgg tgctcaatct gcgggccaag ggaatgcgtc
     1201 tgctccggat gcccgtgttc gcctggatga cgacgatcac ctcgttcctc ctcctcttct
     1261 ccctccccat aatcgcggtc gcactgttca tggtgacctt cgaccgccag ttcggctcgt
     1321 tgttcttcgc cgcgcaggcc ggtggcgacg tgatcctgtg gcagcacctg ttctggctct
     1381 tcggtcatcc cgaggtctac atcctgatcc tccccgccat ggggatcgtc tccgagatcc
     1441 tccccacctt ctcccgcaag ccgctgttcg ggtactcggc cgtggtgttc gccggtgccg
     1501 ccatcgcctt cctcggcttc ggggtgtggg cccaccacat gttcgcctcc ggcatcaccc
     1561 cggtcgccca ggccgccttc ggtctcgcca ccatgaccat cgccatcccg accgggatca
     1621 agatcttcaa ctggctcggc acgatgtgga tgggccggat ccggctcgcc acgccgatgc
     1681 tgtttgccat cggctttgtc gccatgttca ccattggagg gctctccggt gtcacccatg
     1741 ccgtagttcc ctccgactgg cagcagacgg acacctatta catcgtggcc catttccact
     1801 atgtgatctt cgggggagcc gtgttcgggc tcttcggggg tctctactac tggttcccca
     1861 agctgaccgg ccgcgtcatg aacgagcgtc tcgggaagat ccatttctgg ttgatgctgc
     1921 tcggcttcaa cctgaccttc ggaccgatgc actggttggg acttcaaggg atggtgcgac
     1981 ggacctggaa gtacgcgccg gaaaccggtc tcgagccatg gaacgtcgtc gtcaccatcg
     2041 gcgcgttcat catcgccctc tccattctga tcttcatgtt caactggttc acgagccgtc
     2101 gtcgcggcga ggcctccggg ctggacccat gggatgcccg caccgtcgag tggatgtcgc
     2161 ccaaccccac tcccgagtac aacttcgcgg taccaccggt ggtgaccagc ctggaccact
     2221 tctggcacgt caagtacgag gaggacgccg agggccgggc ggtgcgcaag gaggacggcg
     2281 acgagatcgt ggccaccatc gaacacgagc agctcaaccc cgaggcgcct atccatctcc
     2341 ccaacccgtc gtactttccg ttcttcttcg ccctcggcct tccgctcatg ttctacgggg
     2401 tcatctacca cgaggcgatg tggggcaaag gtctgatcct ggtcgggctg atcctcagcc
     2461 tgtccgctgt catcggctgg gctatcgagc cgctcgagga gccgcatgac gagcacgagg
     2521 tacccgcgga acacgatgag ggggaccacg gcgcaggaga gctagagccc gccgtcgagg
     2581 cggaggcatc ggagtcgatc gatgactgag ctcacccacg aggccgacct ggatcaggct
     2641 catcccgagc acgaggggac tggagtcgaa gaccgcaagc tggggatgtg gctcttcctc
     2701 agctccgagt tcctcttctt cggggccctg atctcgaact acctgctcta ttcgcaccgg
     2761 cccgggttcg aggggatcta cccggccgag atctatgaca tcccgttcac gtcggtgagc
     2821 tccttcgtgc tgctcatgag ctcgttgacc atggtcctct cccacaacgc actctcccgt
     2881 ggagatcaga acggaaccag gacctggctg ttcgccaccg cggcgctggg tgcggtgttc
     2941 ctcgggggcc aggtcttcga gttcaccgag ttcatcaccg cctacgggat gaagatgagc
     3001 agcaatccgg cggcgtcggc cttctacctg ctcaccggct ttcacgggac ccacgtcg
//
LOCUS       DASTXO010000006         2049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1584521, whole genome shotgun sequence.
ACCESSION   DASTXO010000006 DASTXO010000000
VERSION     DASTXO010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584521"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VFU96_00100"
     CDS             <1..206
                     /locus_tag="VFU96_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111683.1"
                     /translation="EVLRVLARGGRLLIVERKLKRGAGHGLDEESADRLGMSLDAHGY
                     MTIEVDTMRVGRAEYLVVSAIKP"
     gene            complement(234..623)
                     /locus_tag="VFU96_00105"
     CDS             complement(234..623)
                     /locus_tag="VFU96_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5111684.1"
                     /translation="MLGWLDDIEKATLDNENFREVLFTGKHTQLTVMTLAPGEEIGWE
                     AHGTHDQFIRIEHGQARVEFGRTEDAVDETHEVDDDWAFIVPAGVWHNVVNTGDAKLK
                     LYSLYSPPEHPDGTVHRTKAEADAAEH"
     gene            701..2014
                     /gene="hisD"
                     /locus_tag="VFU96_00110"
     CDS             701..2014
                     /gene="hisD"
                     /locus_tag="VFU96_00110"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019586010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEU5111685.1"
                     /translation="MPRPTTPSYLKEPIPTPEEVSREVTATVSQIIAEVEREGTEAVR
                     RHSVRLDGWDPREFRVGAAEIEAAYAAMEPTVEESARFLIDQVTNFASLQRKTLIDFE
                     TETLPGVFLGQRHIPVASVGAYSPGGLYPLIASAVMTVATAKVAGVPRVVAAAPPRDE
                     QGMHPPQLWAMAESGAEEIFCVGGVQALAGMAFGLEGSDPVDMIVGPGNAYVAEAKRQ
                     LFGKVGIDLLAGPTEIMIIADESADPSVVACDLLGQAEHGPTSPAILATTSEALGRAA
                     IEQVDWWLERWPTADVAGAAWRDFGTVILCDDDAGLVEVADEIAPEHLEIQTSDPDWF
                     VSRLTNYGSLFIGTHATVAYSDKAIGTNHTLPTRRAARYTGGLWVGKFLKTVTTQRCE
                     PRGSLQVAEPAAVVADAELMAGHAISSRLRLNPEFFPDILEGTDV"
BASE COUNT          352 a          643 c          693 g          361 t
ORIGIN      
        1 acgaggttct acgtgtgctc gccagaggtg gccggctact gatcgtcgag cgaaagctga
       61 agcggggtgc cgggcacgga ttggatgagg agagtgccga tcggctcggt atgtcgctcg
      121 atgcccacgg gtacatgacg atcgaggtcg acacgatgcg ggttggtcga gccgagtatc
      181 tggtggtgtc agccatcaaa ccctgacgcg agaccccgag atcggtgccc gggtcagtgc
      241 tcggcggcgt cggcctccgc cttggtgcgg tggacagtgc cgtcgggatg ctcgggtggt
      301 gagtagagcg aatagagttt caatttcgcg tccccggtgt tgacgacgtt gtgccagaca
      361 ccggcgggca cgatgaaggc ccaatcgtcg tcgacttcgt gggtctcatc cactgcgtcc
      421 tcggttcgac cgaattcgac ccgcgcctga ccgtgctcga tacggatgaa ctggtcgtgg
      481 gtgccgtggg cctcccaacc gatctcctcg ccaggagcga gggtcatcac cgtcagctgg
      541 gtgtgcttcc cggtgaagag cacctcccgg aagttctcgt tgtccagcgt ggccttctcg
      601 atgtcgtcca accatccgag catgtggcct cctcgcgagt atttcgatgg tacgcgttac
      661 ccggattcgc ccaccttgca acatatagaa tccggccccc gtgccgcggc cgacgacccc
      721 gagctatctg aaggagccga tcccgactcc agaagaagtg tctcgagagg tgacagccac
      781 ggtgtcacag ataatcgccg aggtggagcg ggagggcacc gaagctgtcc ggcggcactc
      841 cgtgcgcctc gacggctggg atccgcgaga gttcagggtc ggggctgccg agatcgaggc
      901 cgcctacgcg gccatggaac cgacggtcga ggagagtgcg aggttcctga tcgaccaggt
      961 caccaacttc gcctcgctgc aaaggaagac cctgatcgac ttcgagacag agacactccc
     1021 aggtgtgttc ctcggtcaga ggcacatccc cgtggcctcg gtgggggcgt actctcccgg
     1081 cggcctctac ccgctgattg cctcggcggt gatgacggtg gcaactgcca aggtggccgg
     1141 ggtgccacgg gtggtcgcag cggccccacc acgcgatgag cagggaatgc accctcctca
     1201 actttgggcc atggccgagt caggagccga ggagatcttc tgcgtgggcg gggtacaggc
     1261 ccttgccggc atggcgttcg ggctagaggg ctccgacccg gtcgacatga tcgtcgggcc
     1321 aggaaacgcc tacgtggccg aggcgaaacg acagctgttc ggcaaggttg gcatcgatct
     1381 cctggccggg ccgacggaga tcatgatcat cgccgacgag agcgccgacc cctccgtcgt
     1441 ggcatgtgac ctcctcggtc aggccgagca cgggcctaca tcgccggcga tcctggccac
     1501 gaccagcgag gcgctgggac gtgccgcgat cgagcaggtg gattggtggc tggaacggtg
     1561 gcctacggcc gacgtcgccg gcgccgcctg gcgtgatttc gggaccgtca tcctctgtga
     1621 tgatgatgcc gggctggtcg aggtcgccga cgagatcgcc cccgagcatc tcgagatcca
     1681 gaccagcgac ccggactggt tcgtgagccg tctgaccaac tacggcagcc tcttcatcgg
     1741 gacccatgcc accgtcgcct attcggacaa ggcgatcggg accaaccaca ctctgcctac
     1801 cagaagggcg gcccggtaca ccggggggct gtgggtcggg aagttcctca agaccgtcac
     1861 cacccagcgg tgtgagccca gagggtcgtt gcaggtcgcc gagccggcgg cggtagtcgc
     1921 cgacgccgag ctgatggccg gccacgcgat cagttcccgt ctccgtctca accccgagtt
     1981 cttcccggac atcctggagg gcaccgatgt ctgaccgtgc ggccagcagc gtcccggtga
     2041 gcatcgtca
//
LOCUS       DASTXO010000007         3181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_221452, whole genome shotgun sequence.
ACCESSION   DASTXO010000007 DASTXO010000000
VERSION     DASTXO010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221452"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VFU96_00115"
     CDS             <1..391
                     /locus_tag="VFU96_00115"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEU5111686.1"
                     /translation="IADAVGADAVVAADPDTDPFSPNVVRASTGAIFSVPLGVADLDA
                     AVAWVRHHGARITAADPEASDLLWEADLTGPVALLVGAEHTGLTGPARAAADATVSLP
                     MRGSTDSLNVSVSMAVLAYEALRQRSA"
     gene            432..2069
                     /locus_tag="VFU96_00120"
     CDS             432..2069
                     /locus_tag="VFU96_00120"
                     /inference="COORDINATES: protein motif:HMM:NF005907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HEU5111687.1"
                     /translation="MPALLQPSFDDLSTPLFDVTFCVLDLETTGGSPRDCAITEIGAV
                     KYRGGDLLGTFQTLVDPGLPIPPLITILTGITEAMVVEAPDIATALPTFLEFIGDAVI
                     VGHNIRFDISFLNAAAALHGYPPLGNDTVDTAGLGRRLVRDEVRNLRLQTLAAHFRSP
                     VTPIHRALDDARATAHVLHGLLERAGSLGVTNLDDLLQLPRARGSAYYSKIALTEALP
                     RQPGVYLFRDRHGTPIYVGKATNLRARVRQYFYGDERRTIGNLMRELHAVDHIVCPTP
                     LEAEITELRLIHTYRPRYNRRSRPPKSSHWVKLTSEPFPRLSVVRTLKTKGRAHLGPF
                     RSKTAADLVVSALWDATMIRRCSSRPGSRSARCAPAQMGVALCPCDGSLDPFEYQREV
                     DQIVEGITHRSDLLLEPLVDRMEQLSSEQRYEEAGWVRDRHEALARAIETSRTWAALE
                     GLGVCELEGEDGSHVVVDHGRLVCTWRTGSNPPLRPAPEASDAAGNDVPDSVETAEEA
                     RLIWNWIKSTPLRLVDATGTLALPMRPVVHLGPRSRR"
     gene            complement(2058..2762)
                     /locus_tag="VFU96_00125"
     CDS             complement(2058..2762)
                     /locus_tag="VFU96_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016188625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111688.1"
                     /translation="MSTEIVIDYRFRGPPNSANGGYACGVVGEAIDGVATATLRRPPP
                     LDVPLRLVHDDQGVRLLAGEDVVGEAVPDSLDVVAPTGPSFDSALSASQGYSGFDAHP
                     FPTCFVCGPDRAPGDGLRIFPGRVDGSTVVAAPWVPDESLAGVGGAIERRHVWAALDC
                     PSYFGLAVAPMALLGRLTARIERLPEIGERLVVIGWPMGVEGRKHYAGSALLDASGDE
                     IAAAAATWIEIDRLPT"
     gene            2830..>3181
                     /locus_tag="VFU96_00130"
     CDS             2830..>3181
                     /locus_tag="VFU96_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5111689.1"
                     /translation="MGTHIELEDAGVSLGGQTILRGLTLSIDTAEVLGIAGPNGSGKT
                     TFLRLLATLISPDEGSGRVLGAALGTPEVYGIRRNIGLIGHLPTVVGELTLRENLEHA
                     TRLFGGETSRIERAL"
BASE COUNT          552 a         1118 c          980 g          531 t
ORIGIN      
        1 tattgccgat gccgtggggg cggatgcggt cgtcgccgcg gaccccgaca ccgacccctt
       61 cagccccaac gtggttcgag cctccaccgg agccatcttc tcggtgccat tgggagtcgc
      121 cgacctggat gccgccgtcg cctgggttcg gcaccatgga gcgcggatca cggccgccga
      181 ccctgaggcc tcggacctcc tctgggaagc cgacctcacc gggcctgtcg ccctcctcgt
      241 gggtgccgag cacaccgggc tcaccggacc ggcacgggct gcggcggacg cgactgtctc
      301 cctacccatg cgcggatcga ccgactccct caacgtatcg gtctccatgg cggtgcttgc
      361 ctatgaggcg ctccgccaga ggtctgctta gtccttgcac cgaacatatg ttcgattagg
      421 ctcggaaggg cgtgcccgcc ctgctccaac ccagcttcga tgacctgtcg acgccgctgt
      481 tcgatgtcac cttctgcgtc ctcgacctgg agacgacggg tggatcgccc cgagattgcg
      541 ccataacgga gatcggtgcg gtcaagtacc ggggcggcga cttgttgggg acatttcaaa
      601 ctctggtcga tcccgggctg ccgatacctc ccttgatcac gatcctcacc ggcatcaccg
      661 aagcgatggt ggtcgaggcc cccgatatcg cgactgctct cccgaccttc ctcgaattca
      721 tcggcgacgc ggtcatcgtg gggcacaaca tccggttcga tatctcgttc ctcaatgcgg
      781 cggccgccct tcacgggtac ccgcccctgg gcaatgacac agtcgacacc gccgggctgg
      841 gacgacgcct ggtgagggac gaggtgagga acctgaggct gcaaaccctc gccgctcatt
      901 tccgcagccc ggtgacaccg atccaccgcg ccctcgacga cgcccgggcc acggctcatg
      961 ttcttcatgg gctgctggag agggcgggga gcctcggtgt gacgaatcta gacgacctgc
     1021 ttcaactccc ccgggcccgg ggctccgcgt attactcgaa gatcgcgttg accgaggccc
     1081 tgccccgaca accaggggtc tacctgttcc gggaccggca tggcacaccc atctacgtgg
     1141 gcaaggcgac caatctcagg gcgagagtgc gccaatactt ctatggcgac gagcgtcgga
     1201 ccatcggcaa cctcatgcgt gagctccatg ccgtcgacca catcgtctgt ccgacacccc
     1261 tggaagctga gatcaccgag ctccgcctca tccacaccta ccggccccgc tacaaccgcc
     1321 gatcccgacc ccccaagtca tcacactggg tgaaactgac gtccgagccg ttcccgaggc
     1381 tctcggtggt caggacgctt aagacgaagg ggcgagccca tctcgggccg ttccgatcca
     1441 agaccgcggc cgacctggtg gtgtccgcgc tgtgggacgc gaccatgatt cgacgctgct
     1501 cctcccgtcc aggcagcagg tcggctcggt gtgccccggc ccagatgggt gtcgcgctct
     1561 gcccatgcga cggcagcctc gaccctttcg aataccagcg agaggtcgat cagatcgtcg
     1621 aagggatcac ccatcgatcc gacctactgc tcgagccgct cgtcgaccga atggagcaac
     1681 tctcctccga gcagcggtac gaagaggccg gatgggtccg agaccgtcat gaggctctgg
     1741 cccgggccat cgaaacgagc cgaacctggg ccgccctcga ggggctgggt gtatgtgagc
     1801 tggaaggaga ggacggttct catgtcgtgg tggaccacgg gcgtctcgtc tgcacctggc
     1861 ggaccggatc caacccgcct ctgcgtcccg cgcccgaagc gagcgatgcg gcgggaaatg
     1921 acgtcccgga ttccgtggag acagccgaag aggcccggct gatctggaat tggatcaagt
     1981 ccacgccgtt gcggctcgtc gacgccaccg gcactctcgc actgccgatg cgacccgtcg
     2041 ttcatctcgg gcctcgatca cgtcggtagc cgatcgatct cgatccaggt cgccgccgcg
     2101 gctgcgatct cgtcgccgct ggcatcgagc agcgccgatc ctgcgtagtg cttccgccct
     2161 tccaccccca tcggccaacc gatgacaacc agacgttcgc ctatttcggg caaccgctcg
     2221 attcgggccg taagacggcc gagaagggcc atcggggcga cggccagtcc gaagtacgac
     2281 gggcaatcga gggcagccca gacatgacgg cgctcgatcg cgcctccaac gccggccagg
     2341 gactcgtcgg gtacccaggg ggcggcgacc acggtcgacc catcgacacg gcccggaaag
     2401 atccgcaacc catcgcccgg cgcccgatct ggcccgcaga cgaaacatgt cggaaacggg
     2461 tgagcgtcga agccggagta gccctgagat gccgacagcg ccgagtcgaa actcgggcct
     2521 gtcggggcaa ccacgtcgag gctgtcaggc accgcttctc cgacgacgtc ctcgccggcg
     2581 agcagtctca ccccctggtc gtcatgcacc agacgcaacg ggacgtccag cggtggaggg
     2641 cggcgcagag tcgccgtggc gacaccatct atcgcctcac caacgacgcc gcacgcgtat
     2701 ccaccgttgg cgctgtttgg cggtccgcgg aagcggtagt cgatgacgat ttccgtgctc
     2761 atcccctgca gaacctaacg accgcctcag caacttgagt ctgacccggc acccaatgag
     2821 actcaccggg tgggcaccca catcgaactc gaggatgccg gtgtctcgct tggtggccag
     2881 accatcctgc gcggcctgac cttgtccatc gacaccgccg aggtcctcgg aatcgccggc
     2941 ccgaatggat cgggcaagac gaccttcctt cggctcctgg ccacgctcat ctccccggac
     3001 gaaggcagtg gacgtgtcct gggagcggcg ctcggcacgc ccgaggtcta cgggattcgg
     3061 cgcaacatcg gcctcatcgg ccatctcccc accgtcgttg gcgagctgac gcttcgggag
     3121 aacctcgagc acgccactcg cctgtttggc ggtgagacct ccaggatcga acgcgccctg
     3181 g
//
LOCUS       DASTXO010000008         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1252997, whole genome shotgun sequence.
ACCESSION   DASTXO010000008 DASTXO010000000
VERSION     DASTXO010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252997"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VFU96_00135"
     CDS             complement(<1..710)
                     /locus_tag="VFU96_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111690.1"
                     /translation="MTTTNDTTTDRSRSRKWIAVLLVVVSSIFVVISAVAVWAHRTIF
                     DTDVFMATIEPAIEDPQFTTALGDYLTDEITSALDLEERLQEPLADLDAFLTSALFGL
                     LGIDPGTLGFLGQIDRPTLAALAGPIADALNGRIEMRVDQVMESNAMQELIPQVVRRA
                     HQGAVALIGGDIEDTPNISIVEGEVRLNTLPIIGRVLQEVVAELRDVLPDVNVPDAIS
                     DRADEAIAQFKESLGNRVP"
     gene            complement(718..1116)
                     /locus_tag="VFU96_00140"
     CDS             complement(718..1116)
                     /locus_tag="VFU96_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015747029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111691.1"
                     /translation="MTQETSGWAVGWTTFAALMMIMIGFWHAIAGLVALLENEFYVVG
                     SEYVFQFDVTTWGWIHLILGVVVILAGVALFSGAVWARTIGVILAVVSAVAAFAWLPY
                     YPIWGGLIAAAAIFVIWALTAHGRDIVQGV"
     gene            1285..1659
                     /locus_tag="VFU96_00145"
     CDS             1285..1659
                     /locus_tag="VFU96_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020135321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEU5111692.1"
                     /translation="MDLFWSMLWFFLFFAWIWLLITIFSDIFRSEDLSGWAKAFWTIF
                     VVILPWLGVLIYLIARGGKMHERAMAMAVAQEKAQREYIQQVSASGGASSADELEKLA
                     KLQSQGVITSEEFAAQKAKILS"
BASE COUNT          396 a          634 c          628 g          394 t
ORIGIN      
        1 gggacgcgat tcccgagcga ttccttgaac tgggcgatgg cctcgtctgc acgatccgaa
       61 atcgcatccg gcacgttcac atcgggcagc acgtctcgca gctcggcgac gacctcctgc
      121 agcacccgac cgatgatggg caacgtgttc agacgaacct cgccctccac aatggagatg
      181 ttgggggtgt cctcgatgtc tccaccgatc agcgccaccg cgccctgatg agcccttctc
      241 accacttgcg ggatgagctc ttgcatcgcg ttcgactcca tgacctggtc cacccgcatc
      301 tcgatccgcc cgttgagcgc gtcggcgatc gggccggcca gggcggcgag ggtggggcgg
      361 tcgatttggc ccaggaagcc cagggttccc gggtcgatgc cgagcaaccc gaacaacgca
      421 gaagtcagga acgcatcgag gtcggccaac ggctcctgta gccgctcttc cagatccagc
      481 gcgctggtga tctcatcggt caggtagtca ccgagcgcgg tagtgaactg tgggtcctcg
      541 atggcgggct cgatggtcgc catgaagacg tccgtgtcga agatggtgcg gtgtgcccac
      601 accgccaccg ccgagatcac cacaaagatc gaggacacga cgacgagcag gacagcgatc
      661 catttgcggg atctggacct gtccgtcgtc gtgtcgttcg tggtggtcaa gtgctggtca
      721 gacgccttgg acgatgtctc gcccgtgagc tgtcaacgcc cagatgacga agatggccgc
      781 agcggcgatg agcccgcccc agatggggta gtaggggagc catgcaaagg cggccaccgc
      841 gctcaccact gccaggatca ccccaatcgt cctggcccac accgccccac tgaagagagc
      901 tacaccggcg aggatcacga cgacaccgag aatcaggtgg atccaacccc acgtggtcac
      961 gtcgaactgg aacacgtatt ccgagccgac gacgtagaac tcgttctcca agagggcgac
     1021 cagaccggcg atggcgtgcc agaagccgat catgatcatc atcaacgcgg cgaaggtggt
     1081 ccaaccaacc gcccaaccgc tcgtctcttg cgtcatagct cctcctaatt ttccggtcag
     1141 gtcgattctc gccgaccaaa cctagagggt attcgcatga tgtgggcgat tgagggcatg
     1201 ttgaaggatg tagcatcgcc gtgaactgaa taggttggcc gtctcaagtg ccaacaaaat
     1261 gagaaaggtg gcgtcgtgcc cttaatggat ctgttttgga gcatgctctg gttcttcttg
     1321 ttctttgcct ggatctggct gctcatcacg atcttctctg acatattccg cagcgaggac
     1381 ctgagtggct gggcgaaggc gttctggacg atcttcgtcg tgatcttgcc ctggctcggg
     1441 gtgttgatct acctgatcgc ccgtggcggc aagatgcacg agcgtgcgat ggcgatggcg
     1501 gtggcgcaag agaaggcgca gagagagtac atccagcagg tttccgcttc aggcggggcc
     1561 tcgtccgcgg acgagctgga gaagctggcc aagctgcaga gccagggtgt catcacctcc
     1621 gaggagttcg ccgctcagaa ggccaagatc ctcagctgaa atcagtgctg cgatgcccgg
     1681 gctcttcatg gctcgggcat cgtcgcgtct gtgatctgcc ggtccggcga aaagctgttt
     1741 gaagtttggc ggagcggggt aacgaggccg gcttggaaag tcaagaacgt gggacggatc
     1801 tggaaaccgc tcccgcctcc ctcggcgagc aatagcccca ccccgcccgg tccgtcccgc
     1861 ttcttggcct tccaccctcc ccccgggccc accgtctgtt cagtagtact cgaggtactc
     1921 ggcgacttcc cagtcggtcg tgtgttgtct gaaccgttcc cactcggccc gcttcacggc
     1981 cagaaaaccc tcgacatagg ccggtgagaa tgcttcgacg aaatccttgt ccgcctccaa
     2041 cgcgtcgagg gc
//
LOCUS       DASTXO010000009         2232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_737333, whole genome shotgun sequence.
ACCESSION   DASTXO010000009 DASTXO010000000
VERSION     DASTXO010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737333"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VFU96_00150"
     CDS             complement(<1..714)
                     /locus_tag="VFU96_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014139399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5111693.1"
                     /translation="MAVIGSTRVVVDGEVRPASIRIEHGTIVEIGAARPEIDFGDMVV
                     MPGLVDSHVHVNEPGRSHWEGFSTATKAAAAGGTTTIVDMPLNSIPPTVSVDALSEKR
                     AAAEGKLWVDVAFWGGFIPGSEDQIRPLVAEGVSGFKSFLVDSGVDEFPSVSPDQLAG
                     ALPVMRELGVPSLVHAEDPARILRLSGDPTIYRSYLDTRPADGEAAAVATVAGLARAT
                     GARVHVLHVSSAEAMQAVAE"
     gene            819..1346
                     /locus_tag="VFU96_00155"
     CDS             819..1346
                     /locus_tag="VFU96_00155"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEU5111694.1"
                     /translation="METVSSSPPDRTINYAFILGGVTALIFGIILLVRQDEALSLLMV
                     ILGLWWLIQGAFMIFAVFVDRTDMWWKLIIGVLGVVAGFLVLTNPGEAADVFQGVIGV
                     VLGIIGIVVGVTAIIGSFRGGGVGAFVFGAVSVLIGLLVLLNAQFSTELLITLFAVLL
                     LIDGVAGIYLGVKNR"
     gene            1438..>2232
                     /locus_tag="VFU96_00160"
     CDS             1438..>2232
                     /locus_tag="VFU96_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5111695.1"
                     /translation="MPDFLSRHNDVLAPVLAFDTDIHAAKAEGIWITDVDGKRWADFA
                     CGTAVTNLGHNHPAVLEAAHAQLDNLVHSGCIVRYDSIVEAAERLRAITPPGIEKFSF
                     ANSGAEAVEAAVKLAKHTTGRQGVVVFRGAFHGRTMGSVTYTTSNARYRDGYHPVLGS
                     VFVTPYPHPYRWGMTEDEATALSLNELQMMFKHVVTPKNIACFLIEPVQGEGGYYPAP
                     ARFLDALREMADEHGIKLLYDEVQSGFGRTAEWFATDHYDAKPDIMA"
BASE COUNT          397 a          757 c          678 g          400 t
ORIGIN      
        1 ctcggctact gcctgcatcg cctctgccga cgacacgtga aggacatgga cccgcgcccc
       61 ggtagcacgg gccagacccg cgaccgtggc caccgccgcg gcttcgccgt cggcgggacg
      121 ggtgtcgagg tacgaccggt agatggtcgg atcgccgctc agccggagaa tgcgggcagg
      181 gtcctcggca tggaccaagg aaggcacccc cagctcacgc atcaccggca gtgccccggc
      241 aagctgatcg ggactcaccg atgggaactc gtctaccccc gagtcgacca ggaaggattt
      301 gaagccagag actccctcgg cgaccaaagg gcggatctga tcctccgatc ccgggatgaa
      361 gccaccccag aaggcgacat cgacccagag tttcccctcg gcggcagccc tcttctcgga
      421 cagggcgtcg acgctgacgg tagggggaat cgaattgagc ggcatatcga cgatggtggt
      481 cgtcccgccg gccgcagcgg ccttggtggc agtgctgaat ccctcccagt gcgatctccc
      541 gggctcgttc acatgaacat gggaatcgac caacccgggc atcaccacca tgtcaccgaa
      601 atcgatctcg ggtcgggccg cgccgatctc gacgatcgtg ccatgttcga tccggatcga
      661 tgccgggcgc acttccccgt cgacgaccac cctggtgctg ccgatgactg ccatctgggg
      721 ttgaaagcta ccggtgccac cgttcgttct tgccaccgcg gcgaccgacg cggtaagtag
      781 cgttatccca cgtcccccaa ccaagagaag gagtcgcgat ggagacggtg agtagcagcc
      841 caccggatcg gacgatcaac tacgccttca tcctcggagg tgtcaccgcc ctcatcttcg
      901 ggataatcct cctcgtccga caggatgagg cgctgtccct gctgatggtg atcctggggc
      961 tgtggtggct catccaaggc gcattcatga tcttcgccgt gttcgtcgac cgcaccgaca
     1021 tgtggtggaa gctgatcatc ggcgttctgg gtgtcgtggc cggattcctc gtgctgacca
     1081 accccggtga ggccgccgac gtcttccagg gtgtgatcgg cgtcgtgctc gggataatcg
     1141 ggatcgtggt cggtgtcacc gccatcatcg gctcgttccg cggcgggggc gtcggtgcct
     1201 tcgtgttcgg ggcggtcagc gttctcatcg gccttctcgt cctgctcaac gcacagttct
     1261 ccacagaact cctcataacc ctgttcgccg tcctcctcct catcgatggc gtggccggca
     1321 tctacctggg cgtcaagaac cgataaccac acgttcccga tgcagggcgt cacaccggtg
     1381 acgccctgcc cgatttctcc acttcttccg gcccaaacca gccggtagca tcccccgatg
     1441 cccgatttcc tgagcagaca caacgacgtt ctcgcccccg tgctcgcctt cgacacggat
     1501 atccacgcgg ccaaggccga ggggatctgg atcaccgacg tcgacgggaa gcgctgggcg
     1561 gacttcgcct gcggaaccgc ggtgaccaac ctgggtcaca atcacccggc cgtgctggaa
     1621 gcggctcacg ctcagctgga caacctggtg cactctggat gcatcgtccg ctacgactcc
     1681 atcgtcgagg ctgccgaaag actgcgggcg atcaccccgc ctgggatcga gaagttcagc
     1741 ttcgccaatt ccggtgccga agcggtcgag gcggcggtga agctggccaa gcacaccacc
     1801 ggccgacaag gtgtggtcgt tttccgagga gcattccacg gccggaccat ggggtcggtc
     1861 acgtacacca cctcgaacgc caggtatcgc gacgggtacc accccgtgct cggctcggtg
     1921 ttcgtcaccc cgtatccgca cccgtatcga tggggtatga ccgaggacga ggccaccgcc
     1981 ctctccctca acgaattgca gatgatgttc aagcacgtgg tgacgccgaa gaacatcgcc
     2041 tgcttcctca tcgagccggt gcagggtgag ggtggctact accccgcccc ggcccggttc
     2101 ctcgatgcgc tccgcgagat ggccgatgag cacgggatca agctcctcta cgacgaggtc
     2161 cagtcgggct tcgggcgcac cgccgagtgg ttcgccaccg accattacga cgccaagccc
     2221 gacatcatgg cc
//
LOCUS       DASTXO010000010         5655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_147966, whole genome shotgun sequence.
ACCESSION   DASTXO010000010 DASTXO010000000
VERSION     DASTXO010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147966"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VFU96_00165"
     CDS             <1..379
                     /locus_tag="VFU96_00165"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5111696.1"
                     /translation="DPSIFRQVLHLLVGNAIRHGGNRVAIWAVEEGGSMRISVSDDGP
                     GLPDGTGAQVFQRYVDLAEQARSTVRAGSGFAVIRGLSEQIGAELGYKRDPSWTHFSV
                     SLPTAPAPVGSLSDRVPLQAGAR"
     gene            383..1288
                     /locus_tag="VFU96_00170"
     CDS             383..1288
                     /locus_tag="VFU96_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111697.1"
                     /translation="MRGPSVKWGVLVALIVVFLAPGVALAEQENYYLGPDGVPRAQLM
                     LNPLGGEMPNYDRGRDVEPGLLLQRSTRGLAEDDAARFQHWQIDVSEQRLTGFPSMVV
                     WSAADMFEAEKQGVFTVYLLDCDTKGVSCENLGSKAATVDTGAGGTWVETIVDFPALD
                     HRFAEGRHLGVRIVVSSASEADLIFAYGYAAQRSRLTLHAEQPIRTLESTVTQTPLTA
                     GAADFGAMVAATAATPEAPVAVAPAAEPGSFWPWLLTLVISTIGLVSVGALLLPSLTK
                     PGRHEAPPEEPPSGRQTTRISVSAP"
     gene            complement(1255..1674)
                     /locus_tag="VFU96_00175"
     CDS             complement(1255..1674)
                     /locus_tag="VFU96_00175"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5111698.1"
                     /translation="MEDSSGPLNRALERIGDRWSLLIIDSLQSGPLRFSDLSERVAGI
                     APNILSSRLSRLEGEGVIVSSPYSERPPRHVYELTGAGRDLAGALRLLAHWGDRQSDE
                     GEAIRHELCGTPLEAHWYCASCDRIIDQGAETEIRVV"
     gene            1766..2647
                     /locus_tag="VFU96_00180"
     CDS             1766..2647
                     /locus_tag="VFU96_00180"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU5111699.1"
                     /translation="MILTEIGETVGAVAGSTGGSVVGIGGHWGMGSGVVLDDQLVLTS
                     AHNVGPERATVTFLDGRVEQARVLGIDVDADLAVLSVDTGDVGAITWLSEDGVPEIGM
                     PVVGLANPGGRGLRATLGFVASAARSFRGPRGRRLAGGFEHTALAPPGASGGPVVDLE
                     GKLVGINTRRLGRGFYLAQAATTGMRDMVTALRSGDGVEPRRLGVGLAPVEVTRRLRR
                     SMGLDEADGLLVRLVEEGSPAEVAGVREGDVLVSAGDQTTSDVDDLHRVLAADGDSLD
                     LGLVRVNDRLTVTARFS"
     gene            2644..3636
                     /locus_tag="VFU96_00185"
     CDS             2644..3636
                     /locus_tag="VFU96_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU5111700.1"
                     /translation="MSPDRPDGISEGQAALDAYSAVVTSVARVLLPTVVALTTRRRGR
                     RGMWHEGSGSGVVISEDGFMVTSAHVVEGSTRGGASFVDGWESGFDVVGRDGLSDLAV
                     LKLEGSGAPAAELGDASSLEVGQLVVALGNPLGFSGSVTAGVVSALGRSIAANSEGHS
                     RLVENVIQTDASLHPGNSGGPLADSMGRVVGVNTAVVGPMIGQGLGLAVPIDEATKTI
                     IAELIKSGRVRRAYLGVAGGTRPLPPRLAERLGRERGIEVVEVVEGSPAQRAGIRPGD
                     ILVASEGEPLRDARDLQRMMTGDRVGGSVEFTVIRGGAISTIATQPVELESERT"
     gene            complement(3641..>5655)
                     /locus_tag="VFU96_00190"
     CDS             complement(3641..>5655)
                     /locus_tag="VFU96_00190"
                     /inference="COORDINATES: protein motif:HMM:NF033681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ExeM/NucH family extracellular endonuclease"
                     /protein_id="HEU5111701.1"
                     /translation="FDGTAVVVEAVVVGDFQGRGALRGFYLQEEDGQVDGEPLTSEGI
                     FVSDASFGIDVAKGDLVEVVGTVDEFNGSTQISTVTSVSVVSTGNAVTPANVILPVAA
                     ITDLEALEGMSVQLPQTLTISEFFNYDRFGEVVLSTERQFQPTALFEPGSPEAGQTAA
                     ANALSRITLDDGRTAQNPDPAIHPNGAEFNLGNLFRGGDTVTNVVGVLDFAFDLYRIH
                     PTQGADFAVQNPRPAEHPTVGGSLEVASFNVLNYFSTIDTGVFICGPLENQECRGADD
                     AGEFTRQKDKIITALAAIDADVVGLMEIENHPTDEALADLVIGLNGRMGAGTYAYIGS
                     GSIGPDAIRIALIYKPSRVTPVGADAVLDTDEFLDPNNLGMAQNRPAQAQTFRENATG
                     ELFTVVVNHLKSKGSECGLGDDDPVQGNCNLTRALSAAVLVDWLDTDPTGSGDPDLLV
                     IGDLNSYDKEDPIDALVAGADDTTGTEDDFADLLLQFEGEKAYSYLFDGQLGYLDYAL
                     ANGSLQEQVTGSAVWHINADEPDLIDYDTTFKQDAQDAIYAPDPYRSSDHEAVIIGLG
                     LDQTPPVVTAELDPIAATVNTGLFVVDFTCHDAVDPNPDCVADLNGIPVADGQIVQLV
                     RAPGRPVTLRLGQVLFMKDESFTLTVIGTDERGNSTTTTVEPVFRN"
BASE COUNT          890 a         1751 c         1965 g         1049 t
ORIGIN      
        1 cgacccgagc atcttccggc aggtccttca tctcctggtc ggaaatgcaa tccgccacgg
       61 tggcaacagg gttgcgatct gggccgtgga ggagggtggc tcgatgagaa tcagcgtctc
      121 cgacgacggg cccgggcttc ccgatggcac cggcgctcag gtgttccagc gttacgtcga
      181 tctcgccgaa caggcccgga gcacggtgcg ggcgggttcg ggcttcgccg tcatccgggg
      241 gctcagcgag cagatcggcg ccgagttggg ctacaagaga gatccgagtt ggactcattt
      301 ctcggtgagc ctgccgacgg cccctgcccc ggttggttcg ctctcggacc gggttccgct
      361 ccaagcaggg gctcgttgag acatgcgcgg acccagcgtg aaatggggcg tgctggtcgc
      421 cctcatcgtg gtgtttctcg ctccgggcgt cgccctcgct gagcaggaga attactacct
      481 gggacccgac ggggtccctc gtgcccaatt gatgctcaac ccgctcggtg gggagatgcc
      541 caactacgac cgagggagag acgtagagcc cggcctgctc ctccaacgct cgacccgcgg
      601 gttggcggaa gacgacgccg cccgcttcca gcattggcag atcgatgtca gcgaacagag
      661 gctgacggga tttccctcga tggtggtctg gagtgccgcg gacatgttcg aggcggagaa
      721 gcagggggtc ttcacggtct atctcctcga ctgcgacacg aagggcgtct cctgcgagaa
      781 cctcggatcc aaggcagcca cggtcgacac cggcgccggt gggacgtggg tcgagaccat
      841 cgtcgatttc cccgcgctcg accatcggtt cgccgagggg cggcatctcg gcgtgcggat
      901 cgtggtttct tcggcgtcgg aggcggacct gatcttcgcc tatggctatg ccgcccaacg
      961 gagccgtctc actctccacg ccgagcaacc gattcggacg ttggagagca cagtcaccca
     1021 aacaccgttg acggcagggg cggccgattt cggcgccatg gtcgccgcca cagctgcgac
     1081 cccggaagcc ccggtggctg tggctcctgc ggctgagcct ggctcgttct ggccgtggct
     1141 gctcaccctg gtgatctcca ccattgggct cgtctcggtg ggggctttgc tccttccctc
     1201 gttgaccaag ccgggccgcc acgaagcccc tccggaagag cctccatccg gacgtcagac
     1261 cactcggatc tccgtctcgg ccccctgatc gatgatccgg tcacagctgg cgcaatacca
     1321 atgcgcctcg agcggcgtgc cgcacagctc gtgtctgatc gcttcccctt cgtctgactg
     1381 acgatcaccc cagtgggcga gcagacgcaa tgcgccggcc aggtcccgac cggctcccgt
     1441 cagctcgtat acatggcgag gcggccgctc cgagtagggg ctggagacga tcaccccttc
     1501 accctcgaga cggctgagac ggctggagag gatgttgggg gcgatcccgg ccacccgctc
     1561 ggacaggtct gagaacctca gtgggccgct ctgcaatgag tcgatgatca gaagcgacca
     1621 tcggtcgcca attcgctcca aggcccgatt cagcggtccg gagctgtctt ccatcccgcc
     1681 atgttatgcc cgccgccctc gattccctgc gttcaagtgg tggtagtctt gcaaattgca
     1741 agtcactagt gacaggagat gggcgatgat tctgactgag atcggggaga cggtgggtgc
     1801 ggtggcgggc tcgacaggtg gctctgtcgt ggggatcggc ggccattggg gaatgggctc
     1861 gggggtggtc ttggacgacc aactcgtcct cactagtgct cacaacgtgg gcccggagcg
     1921 ggcgactgtg acctttctcg acggccgcgt ggagcaggcc cgggtgctgg ggatcgacgt
     1981 cgacgccgac ctggccgtgt tgagcgtcga taccggtgat gtcggagcga tcacatggct
     2041 gtcggaggat ggggtcccgg agatcggcat gccggtcgtc gggctggcca accccggagg
     2101 ccgagggctc agagcgactc tcggcttcgt ggcctccgcc gctcggtcgt ttcgtggccc
     2161 gcgtggccgc cgcctggccg gaggtttcga gcacaccgca ctcgcccctc ccggggcatc
     2221 gggtgggccc gtagtcgatc tggaggggaa gctggtcggg atcaacaccc gccgtctggg
     2281 acgcgggttc tatctggccc aggcggcgac gacagggatg agggacatgg tgaccgcgct
     2341 gcggtccgga gatggcgtcg agccgcgccg tctcggggtg ggtctggccc cggtcgaagt
     2401 gacccgccgt cttcgtcgat cgatgggcct cgacgaggcg gacggtcttc ttgtgcggtt
     2461 ggtcgaggag ggctctcccg ccgaagtcgc tggggtgcgg gaaggtgatg tgctagtcag
     2521 cgcgggtgac cagacgacgt ccgatgtcga cgacctccat cgtgtccttg ccgcggatgg
     2581 cgattcgctg gatctcgggt tggttcgtgt caacgacagg ctgaccgtga ccgcgcgctt
     2641 ctcgtgagcc cggaccgccc cgatggcatc tccgagggtc aggcggcgct ggatgcgtat
     2701 tccgccgtgg tcacctccgt ggcacgggtt ctcctgccga cggtggtggc tctgaccacg
     2761 cgtcgacgag gccgccgcgg catgtggcat gaaggctccg gctcgggcgt ggtgatcagc
     2821 gaggacgggt tcatggtgac atcggcccac gtggtcgagg gaagcactcg cggaggcgca
     2881 tcgttcgtcg acggctggga atcggggttc gatgtggtcg gtcgcgacgg cctgtctgac
     2941 cttgccgtcc tcaagttgga ggggtcaggg gcgcccgccg ccgagttggg ggatgcctcg
     3001 tcgctggagg tgggccaact cgttgtcgcg ttgggcaacc cgctcgggtt ctcagggtcg
     3061 gtgacggcag gcgtggtcag tgcccttggc cggtcgatcg cggccaacag cgagggtcat
     3121 tcgcgtcttg tggagaacgt catccagacc gacgcctccc tccaccccgg caattcgggg
     3181 ggcccgctgg cggactcgat ggggagagtg gtgggggtga acacggccgt cgtcggtccg
     3241 atgatcggcc agggcctcgg tctcgcagtc ccaatcgatg aggccaccaa gacgatcatc
     3301 gccgagttga tcaagagtgg gagggtccgt cgtgcgtatc tgggtgtggc cggcggcact
     3361 cgccctctgc caccccgatt ggccgagcgc ttgggccgcg agcggggcat cgaagtcgtc
     3421 gaagtggtgg agggaagccc cgcgcagagg gccgggatcc gcccagggga catcctggtc
     3481 gccagcgagg gcgagccgtt gcgggacgcc agggacctgc agcggatgat gaccggagac
     3541 cgcgtcggcg ggtcggtcga attcaccgtg atccgcggcg gtgccatctc caccatcgcc
     3601 acccagccag tcgagctgga gtccgagagg acttagctct ctaattccgg aacaccggct
     3661 cgaccgttgt cgtcgtgctg ttgccgcgct catcggtgcc gatcactgtc agggtgaagc
     3721 tctcgtcctt catgaagagg acctgcccga ggcgcagggt gacgggtctc cccggtgccc
     3781 gaacgagctg cacgatctga ccgtcggcca ccggtatccc attgaggtcg gcaacacagt
     3841 cagggttggg atcgaccgcg tcgtggcagg tgaagtcgac gacgaacaac ccggtgttga
     3901 cggtggcggc gatgggatcg agctcggcgg tcaccaccgg cggtgtctgg tcgaggccga
     3961 gcccaattat caccgcctcg tggtcggatg accggtaagg gtcaggggcg tagatagcgt
     4021 cctgggcgtc ctgcttgaac gtggtgtcgt agtcgatgag gtctggctcg tctgcgttga
     4081 tgtgccacac ggccgatccc gtcacctgct cctggagcga tccattcgcc agcgcatagt
     4141 cgaggtagcc gagctggcca tcgaacaggt agctgtaggc cttctcgccc tcgaactgca
     4201 acaggagatc ggcgaagtcg tcctcggtac ccgtcgtgtc atcggctcca gcgacgagag
     4261 cgtcaatggg gtcttccttg tcgtaggagt tgagatcccc gatgaccagc agatctggat
     4321 cgccacttcc tgtcgggtcg gtgtcgagcc aatcgacgag cactgctgcc gagagagccc
     4381 gggtcaggtt gcagttcccc tgcacgggat cgtcatcgcc gaggccacat tccgagccct
     4441 tcgacttgag gtggttgacc acgacggtga acagctcgcc ggtggcgttc tcccggaagg
     4501 tctgagcctg agcaggccgg ttctgggcca tccccaggtt gttgggatcc aggaattcgt
     4561 cggtatcgag caccgcgtcg gctccgaccg gagtcacccg cgaaggcttg tatatgagag
     4621 cgatcctgat cgcatccggc ccgatcgatc ctgatccgat gtaggcatag gtcccggcgc
     4681 ccatccgtcc gttgagaccg atcaccagat cggcgagggc ctcgtcggtg ggatgattct
     4741 cgatctccat cagcccgacg acatcggcgt cgatggcggc cagggcggtg atgatcttgt
     4801 ccttctgccg ggtgaactcg ccggcatcgt cggccccacg gcattcctga ttctcgagcg
     4861 ggccgcagat gaagacaccg gtgtcaatcg tggagaagta gttcaacacg ttgaatgacg
     4921 ccacctcgag gctgcctccg actgtggggt gctccgcggg acgtgggttc tggacagcga
     4981 aatcggcccc ctgtgtcgga tggatccggt agaggtcgaa tgcgaagtcg agcaccccga
     5041 cgacattcgt caccgtgtcc ccgccgcgga acaggttgcc caggttgaac tcggcgccgt
     5101 tcggatggat cgcagggtcc gggttttggg cggtccgccc gtcgtcgagc gtgatccggc
     5161 tcagggcgtt ggctgctgcg gtctggccgg cttcgggcga accgggctcg aacagggcgg
     5221 tcggctggaa ctggcgttcg gtggagagga cgacctcgcc aaagcggtcg tagttgaaga
     5281 actccgagat cgtcagcgtc tggggcaatt gcaccgacat gccctcgagc gcctcgaggt
     5341 cggtgatcgc ggccaccgga aggatgacgt tggccggggt gacggcgttc ccggtagaga
     5401 ccacggagac cgacgtcacc gtgctgatct gtgtcgagcc gttgaactcg tcgaccgtcc
     5461 cgaccacttc cacgaggtcg cctttggcca cgtcgatgcc gaaggaagcg tcggacacga
     5521 agatgccttc cgacgtcagg ggttccccgt ctacctgacc gtcctcctcc tggaggtaga
     5581 agccgcgcag tgcccctcgc ccttggaagt caccgacgac cacagcctcg acgaccacgg
     5641 cggtgccgtc gaacg
//
LOCUS       DASTXO010000011         2470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1511107, whole genome shotgun sequence.
ACCESSION   DASTXO010000011 DASTXO010000000
VERSION     DASTXO010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511107"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VFU96_00195"
     CDS             <1..555
                     /locus_tag="VFU96_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111702.1"
                     /translation="GSPSSVVALSHYATWSHCIDLILLLAGRPRVISAQQGVVFHSHP
                     ASGSAADLAASFLIDGSASGTLLGTNALDWNFPLFELMVAFEHGRIRLRGLDQEMELL
                     DYAGDIHEIITPSRETSRWDKYDASFDKSISAYLDSVRADDPPPVPGIAGLLELQFEA
                     SLRRSIAEQRLVAPDDEFPIDPVR"
     gene            565..1515
                     /locus_tag="VFU96_00200"
     CDS             565..1515
                     /locus_tag="VFU96_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU5111703.1"
                     /translation="MRYIEITDIGRPVSVIGLGTTSRIFTPAGDDRVADLIDAFLAVG
                     GNCIDTAHIYGSGESEKTLGRWLEQSRRRDEIILITKGCHPHVDEAGQFGPPRVTPEA
                     IHSDLDESLERLATDRVDLYLLHRDDETVPPGPLLEALNEEKARGRIAAFGASNWTVD
                     RIALANEYAAEHGLTGFAVSSPGLSLPRPVEMQFPGTLFADDHTRRWHTQTQLPVLAW
                     TSLAAGFVSGRFRPNDKVVDESTRAFFSEENFERLRRAQEVAERSNASSLEVALAFVL
                     QQQFPVIALVGPSRVENLEGTLGALDLDLTEEEMRYLDLE"
     gene            complement(1516..2445)
                     /gene="bmt"
                     /locus_tag="VFU96_00205"
                     /pseudo
     CDS             complement(1516..2445)
                     /gene="bmt"
                     /locus_tag="VFU96_00205"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="betaine--homocysteine S-methyltransferase"
BASE COUNT          426 a          829 c          770 g          445 t
ORIGIN      
        1 ggatcgccgt cgagcgtcgt tgccctgtcc cactacgcca cctggagcca ctgcatcgac
       61 ctgatcctgc tgctggcagg gcggccgaga gtgatctcgg ctcaacaggg cgttgtgttc
      121 cactcccacc ccgcctcggg atccgcggcc gatctagcag cgtcgtttct catcgatggg
      181 agtgcctccg gcacccttct cggaaccaac gccctggact ggaactttcc cctgttcgag
      241 ttgatggtgg ctttcgaaca cggccgaatt cgactgcgtg gcctggatca ggagatggag
      301 ctgctcgatt acgcgggtga catccacgag atcatcaccc cgagccggga gacctcccgc
      361 tgggacaagt acgacgcctc gttcgacaag tcgatctccg catatctcga ctcggtgcga
      421 gccgacgacc cgccaccggt tcccgggata gccgggctcc tggaactgca gttcgaggcg
      481 agcctgcggc gatcgatcgc cgagcagcgg cttgtggccc cggatgatga gttcccgatc
      541 gacccggtac ggtgacgcgc ctcaatgaga tatatcgaga tcaccgatat cggacggccc
      601 gtctcagtga tcgggctggg caccacttcg aggatcttca cccctgccgg agacgaccgg
      661 gtggccgatc tgatcgacgc cttcctcgcg gtcggaggaa actgcatcga caccgcacat
      721 atctacggtt ccggcgagag cgagaagacc ctgggccgct ggttagaaca atcgcgccgg
      781 cgcgacgaga tcatcctgat caccaaggga tgccacccac atgtggacga ggccgggcag
      841 tttggcccgc cgagggtcac accggaggcg atccactccg acctggatga gagcctggag
      901 aggctcgcga ccgaccgcgt cgacctgtac ttgctgcatc gtgacgatga gacggtgcct
      961 cccggtccgc tgctggaggc gctcaatgaa gagaaggcca ggggccgtat cgctgcgttc
     1021 ggtgcctcca attggaccgt cgaccgaatc gccctggcca acgaatatgc agccgagcat
     1081 ggcctcaccg ggttcgctgt ctcctcgccc ggtctcagcc tgccccgacc ggtcgagatg
     1141 cagttccccg ggacattgtt tgccgacgac cacacccgtc gctggcacac gcagacccag
     1201 ttgccggtgc tcgcttggac ctcgctggcc gccggcttcg tgagtggtcg attccggccg
     1261 aatgacaagg tggtggacga aagcaccaga gccttcttct ccgaggagaa tttcgagagg
     1321 ctacggcggg cacaggaggt ggccgagaga tcgaacgcct catccctcga ggtcgccctg
     1381 gccttcgtcc ttcagcagca gttccctgtg atcgctttgg tcggcccgtc gcgagttgag
     1441 aacctcgaag ggacattggg cgccctggac ctcgacttga ccgaagagga gatgcggtat
     1501 ctcgacctgg agtgattagc ggcgacggcc ttcccgacgg gtcccgaccc cgctcttcgc
     1561 cacggaggcg tccaaccggt ctctgatctc gtcgaagtcc gggcgagcgg catggacgta
     1621 tccgtcgact gcctcacgga tggccgcggt gtggtcgggg gtcgatccac aacacgcccc
     1681 gacgatcctg gcccccgacc gaagggcgag ctcgacatag tcggccatcc cattgggtcc
     1741 gatcgggtag gtgagccccc cttcggcctg atagaggggc atgccgcagt tcgccttgtt
     1801 gatggttggg actcccggcg cgacctcgcc gatggcatgt gccgctgcca ccgcatcgcc
     1861 cgggccgatt ccacagttgg cgccgatcgc ggcgacctgt tcctgactca gccaccagtt
     1921 tcccaagtcc ctgggatgga tccccatcat cgtgtggccc gcggtatcga agctcaatgt
     1981 cgcggcgatc ggcagccctg tgcccgacgc cgcctcgacc gcagcgttca attcctcgaa
     2041 cgacgagatc gtctcgatcc agatcacgtc tgcacctgcc tcggccagag cttcgcactg
     2101 ctccgtgaac acctcgacgc cacgctaatg ggagagggtg ccgagcggct cgaagagctc
     2161 gccggtaggt ccgacgctcc ccgccaccac caccgggcga tcggccagat cggcggcccg
     2221 cctggccact tcgacccctg ccgcgttcag ctcggcgacc ctgtctccaa gatcgtggag
     2281 gtcgaggcgg agccggttcc ctccgaaggt gttggtgagg acgatgtcgg cgccggcatc
     2341 gatgaagcca cggtgcactt tctcgacgag gtcgctctcc tcgacgttga gcaactcggg
     2401 acagcctcca ccttccaagc cgagcgagaa gagggtggtc cccatcgcgc catcggcgac
     2461 cagatatcgg
//
LOCUS       DASTXO010000012         2131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_884860, whole genome shotgun sequence.
ACCESSION   DASTXO010000012 DASTXO010000000
VERSION     DASTXO010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884860"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2108
                     /locus_tag="VFU96_00210"
     CDS             <1..2108
                     /locus_tag="VFU96_00210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5111704.1"
                     /translation="RYGKVAVTGQAVLFHHESATQNQVAAQLTNMNRRRNHQHLAETR
                     GPRLTRSVRRDILAGTGLFSGKTSRTVALTVTQNDPSMGWGDYYTAHELGDAFAAEGW
                     NVIYIERHEDGWYQIDEEIDLLISLLDSYDVSRAPDGAFTIAWVRNWVDRWLEKPWFE
                     TYDMVIASSEKGSDFISHRSRYAPPRIPLATNAKRFRPGPSVPTFESDYVFTGNKWGV
                     ARSLVGLLDVHPGERFQIFGKGWESDPRVSRYWRGSLDYDLLPEVYRSSRIVLDDTAI
                     HTLPYAFMNSRVFDALASGTLVLTNNVEGSEELFGGLLPTYTGREDLRSLLDHYLDDD
                     EARNQLADQLREEVLAHHTYPRRPAEFLHAALEQIERPRCAIKIGVPDREAMPYWGDS
                     HFASGLAAALTQLGMPTEVHILPEWDLAAKQAVDVVIHLRGLSSYTPKPAHLNVLWLV
                     SHPNDVSVRECEKYDLVLVASRRHAEWLQEQTETPVVFMPQATDHRRFHPVEPDSKLA
                     SDVVFLGNSRGQNRPAVKWAIERGLPLAVYGGGWEGRIPARHLKGDLFPNEDLARLYA
                     SARVVLNDHWPDMRDLGFVSNRIFDVFAAGGIIVSDAVAGLDELFGDLVPVFSDPEQL
                     ERTVRDLIADDDRRKAISHEAQALVATEHTFSHRAAQMMDLIQPMLASRPLELESGRL
                     GNETGTGTEAAAPAVLGGP"
BASE COUNT          415 a          683 c          660 g          373 t
ORIGIN      
        1 gacgatacgg caaagtcgcc gtgaccggac aggcggtgct cttccaccac gaatccgcca
       61 cccagaacca ggttgctgcc caactcacca acatgaatcg tcgccggaac caccagcatc
      121 tcgctgagac ccgcgggccc cggctgacca ggtccgtcag acgtgacatc ctcgctggca
      181 ccgggctctt cagtgggaag acgtcgcgca cggtggcctt gaccgtgacc cagaacgatc
      241 cgtcgatggg ctggggcgat tactacacag cgcacgagct cggtgacgcg ttcgcggccg
      301 agggctggaa cgtgatctac atcgagcggc atgaggacgg ttggtatcag atcgacgagg
      361 aaatcgacct gctcatctcg ctgctcgact catacgacgt cagccgtgca ccggatggcg
      421 cattcaccat cgcttgggtg cgcaactggg tcgacagatg gctcgagaaa ccctggttcg
      481 agacctacga catggtgatc gcctccagtg agaaaggatc agatttcatc tcccaccgat
      541 cccggtacgc gcccccccgt atcccgctcg ccacgaatgc gaagcggttc cgtcccggac
      601 cgtctgttcc gactttcgag tcggactacg tcttcaccgg gaacaaatgg ggtgtggctc
      661 ggagcctggt cgggttgctc gacgtccacc cgggcgagcg cttccagatc ttcgggaagg
      721 gctgggagag cgaccctcgg gtgtcgcgat actggagagg gagcctcgat tacgacttgc
      781 tacccgaggt gtaccggtcc tccaggatcg tgctggatga cactgccata cacacccttc
      841 cttatgcgtt catgaacagc cgggtgttcg atgcactggc tagcgggacg ctggtcctga
      901 ccaacaacgt cgaagggtcg gaggagttgt tcggagggct cttgcccacc tacaccggac
      961 gcgaggacct gcgttctctc ctcgatcact atctcgacga cgacgaggca cggaaccagc
     1021 ttgctgatca actgcgtgag gaagtactgg cccatcacac atatccaaga agaccggccg
     1081 aattcctcca cgcagcactg gaacaaatcg agcgtccccg gtgcgcgata aagatcggcg
     1141 tcccggaccg cgaggcgatg ccttactggg gcgacagtca cttcgcctca ggcctggccg
     1201 ccgccctcac tcagttgggg atgccgactg aggtacacat cctgcccgag tgggacttgg
     1261 cggcgaaaca agcggtggat gtcgttatcc acctacgcgg actgtcgagc tacacgccta
     1321 agccggcgca cctcaacgtc ctgtggctgg tgagccatcc caacgacgta tcggtgcgcg
     1381 aatgcgagaa gtacgacctc gtcctggtgg catcccggag gcatgccgag tggctgcagg
     1441 aacagactga gacgccggtc gtcttcatgc cccaggcgac cgatcaccgc agattccatc
     1501 ccgtcgagcc ggactcaaag ctggccagcg atgtcgtttt cctgggcaac agccggggac
     1561 agaatcgccc cgcggtcaag tgggcgatcg aacgagggct tccgctggcg gtgtacggcg
     1621 gcggttggga agggcgcatc ccggcgcgtc acctcaaggg cgacctgttc cccaacgagg
     1681 acctggctcg tctgtacgcc tcggccagag tggttttgaa cgaccactgg ccggacatgc
     1741 gcgacctcgg cttcgtctcg aaccggatat tcgacgtttt cgccgccgga ggcatcatcg
     1801 tctccgatgc ggtggccgga ctcgacgagt tgttcggcga tctggtgccg gtcttttccg
     1861 atccagagca actggagcgg acggtgagag acctcatcgc cgacgacgac cggcgaaagg
     1921 cgatatcaca cgaggcccag gccctagtcg ccaccgagca cacgttctcc catcgagccg
     1981 cacagatgat ggacctcatc caacccatgc tcgcctcccg gccgctcgag ctggagtcgg
     2041 gccggctcgg gaacgagaca gggacaggta ccgaagcggc ggcccctgcg gtgctcggcg
     2101 gcccctgata ggcgggcaac gggaccagac t
//
LOCUS       DASTXO010000013         2270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1216419, whole genome shotgun sequence.
ACCESSION   DASTXO010000013 DASTXO010000000
VERSION     DASTXO010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216419"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VFU96_00215"
     CDS             <1..244
                     /locus_tag="VFU96_00215"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HEU5111705.1"
                     /translation="TRRDRYTCYMEVQRAARIAGGFALLGAGTAMLVLPGPGVLAILG
                     GLTLLEKDIPWAKRVTDRIKPKRAKKSEQETDQLGG"
     gene            complement(229..636)
                     /gene="sodN"
                     /locus_tag="VFU96_00220"
     CDS             complement(229..636)
                     /gene="sodN"
                     /locus_tag="VFU96_00220"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HEU5111706.1"
                     /translation="MRQTTIAHAHCDLFCGVYDPAQAKIEAMSVLKSAEKYQASDDEV
                     FRSRAISIKEERAELVKHHLMVLWVDFFTADHRKEFPDLDDLFWRAILQAGNAKKSVD
                     PADGQKLVDLIDEIAGVFWQTEKAKTAGVYPPS"
     gene            654..1658
                     /locus_tag="VFU96_00225"
     CDS             654..1658
                     /locus_tag="VFU96_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HEU5111707.1"
                     /translation="MLLSRPYPAWSILMQRFQVSEDSMRPTLAPGDEFVATASRTAEI
                     GEVVALPHPDRQDFWLVKRLAAGPGALVDGHGPLARDRAWVISDNPDVATADSRSFGP
                     VPVSSLQPMVTRLDDATFLEALDLLASEDPALAAVVELHGVPRFWSRPAGFATLVWLI
                     MEQQVSLESGAAMYHRLHGLLGAITPERVAGSTEPALREIGVTRQKTRYLLDLGRLVA
                     SGEFDLDALATMPLTEARDLLLGVKGIGPWTADVYLLSALRFPDVFPLGDRALQVGTG
                     EVLGMTSVPNGEELELLSLPWRPIRAVAARIIWHAYLGRRGRAEPPDPTRGHGVARPA
                     "
     gene            1668..2069
                     /locus_tag="VFU96_00230"
     CDS             1668..2069
                     /locus_tag="VFU96_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEU5111708.1"
                     /translation="MTEFVVQMENRPGRLAALTEALAAFGVNIEALAAYGHDGEGTVR
                     LIVDDAATTRRVLSEAALMNEERTVLTAQLPHRPGELARLTRLLADAGVNIDALYVLR
                     SNAEGIEFAISIDQADEAMPELPITGGALVV"
BASE COUNT          376 a          767 c          712 g          415 t
ORIGIN      
        1 aacccggcga gaccgataca cttgctacat ggaagtgcag cgggcagctc gtatcgcagg
       61 gggattcgcc ctgctcggcg caggcaccgc catgctcgtc ctacccggac ccggggttct
      121 cgccatcctt ggcggtctga cgcttctcga gaaagacatt ccctgggcga agcgcgtcac
      181 cgatcggatc aagccgaaga gggccaagaa atctgagcag gagaccgatc agctcggcgg
      241 gtagacgcct gcggtcttcg ccttctcggt ctgccagaac acaccggcga tctcatcgat
      301 gaggtcgacg agtttctgcc catcggccgg atctaccgac ttcttggcat tgcccgcctg
      361 aaggattgcg cgccagaaga ggtcatcgag gtcggggaac tccttgcgat ggtctgcggt
      421 gaagaagtcg acccagagca ccatgagatg atgcttgacc agctcggcgc gctcttcctt
      481 gatcgagatg gccctggagc ggaacacctc gtcgtcagac gcctgatact tctcggctga
      541 cttgaggacc gacatcgcct cgatcttggc ttgggccggg tcgtacaccc cgcagaagag
      601 atcgcagtgg gcgtgggcaa tcgttgtctg ccgcatggca ctccttcgtt gtcgtgttgc
      661 tcagccgacc ataccctgca tggtcgattc tcatgcagcg gttccaggtg tccgaagact
      721 cgatgcggcc gacgctcgct cccggcgatg aattcgtcgc cacggcttcg aggacggcag
      781 aaatcggcga ggtggtcgcc cttccccacc ccgaccgtca ggatttctgg ctggtcaagc
      841 gtctcgccgc cggccccggg gcactggtcg atggccacgg tcccctcgcg cgcgaccggg
      901 cctgggtgat ctcggacaat cccgacgtgg cgacggccga ctcgcgcagc ttcggcccgg
      961 tccctgtctc gtcgttacag ccgatggtca cccgtctcga cgatgccaca ttcctcgaag
     1021 ccttggacct tctcgcctcc gaagatccgg cgctggccgc cgtcgtcgag ttgcacgggg
     1081 tgccgcggtt ctggtcgcgg ccggccggct tcgccacgct ggtctggctg atcatggaac
     1141 aacaggtctc cctcgaatcg ggggcggcga tgtaccaccg cctccatggg ctgctcgggg
     1201 ccatcacgcc ggagagagtc gccggctcga ccgaaccggc cctgcgcgag atcggggtga
     1261 cccggcagaa gaccagatat ctcctcgacc tgggccgttt ggtggcgagc ggcgaattcg
     1321 atctcgatgc tctcgccacg atgccactca ccgaagcgag agaccttctt ctcggggtca
     1381 agggaatcgg cccctggacc gccgacgtct accttctctc ggcgctgcgc ttccccgacg
     1441 tctttcctct cggtgaccga gccctccagg tcggtaccgg ggaggtcctc ggcatgacct
     1501 cggtgccgaa tggtgaggag ctcgaattgc tctctcttcc ctggcgaccg atccgggccg
     1561 tggcggcgag gatcatctgg cacgcctact tggggcgcag agggcgggcc gagccaccgg
     1621 accccacccg tgggcacgga gtcgcgcgac cggcgtagag tcattccatg accgagttcg
     1681 tggtccagat ggagaatcgt ccgggccgac tcgcagcgct gaccgaggct ctcgccgcct
     1741 tcggggtcaa tatcgaagcg ctcgccgcct acggccacga cggggagggg accgtccgtc
     1801 tgatcgtcga cgatgcggcc acgacacgcc gggtgctctc cgaagctgcc ctcatgaacg
     1861 aagagcgcac cgtgctcacc gcgcagctcc cccatcgtcc gggcgagctg gcccggctca
     1921 cgcgcctgct ggcggacgcc ggggtcaaca tcgacgcgct ctacgtcctg cggtcgaacg
     1981 ccgagggaat cgaattcgcg atctccatcg atcaggccga cgaggccatg cccgagttgc
     2041 cgataaccgg tggggcgtta gtggtctaat aacggggcag cgatactctg ccgccccatg
     2101 ttctcatcat ttgcacagtg ggtcagtgcc cacaagatca tctcgatact ggccggcgcc
     2161 gttctcgtcc tcggcatcgg caccggaatc ttcatggcgg ccaacgcctc gacgggcgcc
     2221 accactacga cgagcgagcc ctcgacgacg actgctccca ccacgactct
//
LOCUS       DASTXO010000014         4366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_921815, whole genome shotgun sequence.
ACCESSION   DASTXO010000014 DASTXO010000000
VERSION     DASTXO010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921815"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VFU96_00235"
     CDS             complement(<1..699)
                     /locus_tag="VFU96_00235"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEU5111709.1"
                     /translation="MGSLQWLDRPVLQDPVALVAFEGWGDAGESASQAVSEFLDKLDA
                     ELFAIIDPDDHFDFQVRRPIVELDATGIRSISWPRNELYVARGMEREMVILLGEEPHY
                     RWKSFTNDVVEVMKVTGVRRAVTMGAFIGQVAHTLPVPLVGSANRPDDLAIHGLLPSG
                     YEGPTGILGVLNHALGNVGIDVISVWAAVPHYLSNQEYPPATEALAIKASELCGVTLD
                     IGDLASASRQFLSTV"
     gene            complement(726..2870)
                     /locus_tag="VFU96_00240"
     CDS             complement(726..2870)
                     /locus_tag="VFU96_00240"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018853157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU5111710.1"
                     /translation="MGGMTCATCAVRIERVLLRQEAVEAASVNLAGASALVRVHDGTD
                     VGALSEAVAKIGYTITRRVSGAPQRDMVEHYHSDEEKQWRRFWVAAALTLPAVLLAML
                     GPEAQWSRLAQGALVTPVVFWCGWQFHRVAFRQALHMSANMDTLISLGSLAAYFYSVW
                     ALFSGEHIYFETAGAIVTLITLGRALETRAKGRASEAVHRLLELGAKEARVIVEDEER
                     LLPIDQIIPGDLMVVLPGEKVPTDGMVESGRSSVDESMLTGESLPVDKGPGHLVYGAT
                     VNQRGRLEVRATAIGAETALAGIVGMVEQAQGSKAPIQHLADRVSSVFVPLVILIAIA
                     TTLIWVGLGNEIGAAVQAGVAVLIIACPCALGLATPTAIMVGSGRGAELGILFKRAEV
                     FETAGKVDTVVFDKTGTLTTGVMTLTDLSTSVSEAEFLRHVASLEAASGHPIGMAVAL
                     AADDAGIDLVEPDRVETLPGFGVVGRVEGRDLLVGKAKLLADRGLAVAGIWMEEMARC
                     ENEGKTAFVAGWDGEARGMIAVADTIREGSRTAVARLVGRGIDTLMITGDNHRTAARI
                     GAQIGIAAVHAEVVPGEKADHVAGLQAGGRSVVFVGDGINDAPALTRADIGMAMGSGA
                     DVAVESGDVILLNADTRLVPVAIELAATTLRTIKQNLAWAFGYNAAAIPLAAAGLLDP
                     MIAAAAMALSSVSVVLNALRLRRFEGGGQVSS"
     gene            complement(2911..3120)
                     /locus_tag="VFU96_00245"
     CDS             complement(2911..3120)
                     /locus_tag="VFU96_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEU5111711.1"
                     /translation="MSEVLLQAPEIHCDHCKMSIEDAVGTLDGVQSVEVAIADATVRV
                     AYDDARVELALIKKTIEEQGYAVFG"
     gene            complement(3249..3446)
                     /locus_tag="VFU96_00250"
     CDS             complement(3249..3446)
                     /locus_tag="VFU96_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111712.1"
                     /translation="MTDIVRGAAIKGMTRPSKRFNEGRVCAKKGCTTKLSQYNKAEYC
                     HAHAPVRFPRVRGKIMDGQDA"
     gene            complement(3730..>4366)
                     /locus_tag="VFU96_00255"
     CDS             complement(3730..>4366)
                     /locus_tag="VFU96_00255"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEU5111713.1"
                     /translation="EVETLFHEFGHVLHQNLGRTELARFAGTSVERDFVEAPSQIMQH
                     WVWRADVLRRFARHHETGDSIPDQLVEQLVAARQLNVSIHQLRQLQYGWWDQTMHGSD
                     PQIDFDVVLREGARLGLIPFHEGTFALASFGHLMGGYDAAYYGYMWSEVFGDDMFSRF
                     EEQGVTNPEVGMAYRREVLEKGGSVDADEMLTAFLGREPDNTAFLRKLGIE"
BASE COUNT          806 a         1462 c         1337 g          761 t
ORIGIN      
        1 gaccgtggac agaaattgac gtgaggccga ggcgaggtca ccgatgtcga gtgtcactcc
       61 gcacagctcg gaggccttga tcgcgagcgc ctccgtagcc ggcggatact cctgattgga
      121 cagatagtgc gggaccgccg cccacacgga gatcacatcg atgccgacgt tgcccagcgc
      181 gtgattgagc acaccaagga tgccggtcgg accttcgtat cccgacggga gcagcccatg
      241 aatcgccagg tcgtcgggcc ggttcgctga tccgaccagg ggaacgggaa gcgtgtgagc
      301 cacctggccg ataaaggcac ccatcgtcac cgcccggcgc acccccgtca ccttcatcac
      361 ctcgactacg tcgttggtga acgacttcca ccggtaatgc ggctcctcgc cgagaaggat
      421 caccatctcg cgctccatgc ccctggccac gtagagctcg tttcgaggcc aggagatcga
      481 gcgaatcccg gtcgcgtcga gctcgactat gggccttcgc acctggaagt cgaaatgatc
      541 gtccggatcg atgatggcga agagctctgc atcgagcttg tcgaggaact ctgagaccgc
      601 ctgactcgcc gactcgccgg catcacccca gccttcgaag gcgaccaagg cgaccgggtc
      661 ttgtaggacc gggcggtcga gccattgcaa cgagcccatt tctccaacct actccccgtg
      721 tcgggttagg aactcacttg tcccccgccc tcaaagcgcc tcaaacgcag ggcgttcaac
      781 acaaccgaaa ccgaggagag agccattgcc gcagcggcga tcatcgggtc gagcaaaccg
      841 gcggcggcca gtgggatcgc cgccgcgttg tatccgaagg cccacgccag gttctgcttg
      901 atcgtgcgca gggtcgttgc agccagctcg atcgcgacgg gcacgaggcg ggtgtcggca
      961 ttgaggagga tgacgtcccc ggattcgaca gcgacgtcgg cgcccgagcc catcgccatc
     1021 ccgatatcgg cccgagtcag agccggcgcg tcgttgatcc catcgccgac gaacaccacc
     1081 gacctccccc cggcctgaag cccggcgaca tgatccgctt tctcgccagg caccacctcc
     1141 gcgtgcacag ctgcgatccc gatctgtgcc ccgattcggg ctgcggtgcg atggttgtcg
     1201 ccggtgatca tcagcgtgtc gatgccacgg ccgaccagcc gcgccactgc ggttctcgag
     1261 ccctcacgga tcgtgtcggc aacggcgatc attcccctgg cctctccgtc ccaaccggcg
     1321 acgaaggccg tcttgccctc gttctcgcac cgagccatct cttccatcca gatgccggcg
     1381 acagccaaac cacgatccgc cagcagcttg gccttgccca cgagcaggtc gcgaccttcg
     1441 accctcccga caaccccgaa tccggggagt gtctcgacac gatccggctc caccagatcg
     1501 atgccggcat cgtcggcggc gagggcgacc gccatcccta tcggatgccc cgacgcggct
     1561 tcgagcgatg cgacatgcct gaggaactca gcctccgaga ccgaggtgct cagatcggtg
     1621 agggtcatca ctcccgtcgt cagcgtcccg gtcttgtcga atacgacggt gtccaccttg
     1681 cccgccgtct cgaacacctc tgcccgcttg aagagaatcc ccagctcggc tccccgcccg
     1741 ctgcccacca tgatggcggt cggcgtggcc aggccgagcg cacaggggca ggcgatgatg
     1801 agcacggcca caccggcctg gactgcggcg ccaatctcgt ttccgagacc cacccagatc
     1861 agcgtcgtgg ctatcgcaat gaggatcacc agtggcacga acaccgaaga gacgcgatcg
     1921 gcgagatgct ggatcggcgc cttcgagccc tgcgcctgtt cgaccatgcc cacgatccca
     1981 gccagggcgg tctcggcgcc gatggccgtg gcacggacct cgaggcgacc cctctggttc
     2041 acggtcgcgc cgtagacgag atgtcccggg cccttgtcaa cgggcaacga ctccccggtc
     2101 agcatcgact catcgaccga ggaccggccc gattcgacca tcccgtcggt cgggaccttc
     2161 tctccaggga gaaccaccat gaggtctccc gggatgatct ggtcgatcgg gagcagacgc
     2221 tcctcgtctt cgacgatcac ccgcgcctcc ttggcgccca gctcgaggag acggtgcacc
     2281 gcctccgagg cacgcccctt tgctctcgtc tcgagagctc ggcccagcgt gatgagcgtc
     2341 acgatcgccc cggcggtctc gaagtagatg tgctcaccgc tgaacagggc ccataccgaa
     2401 tagaagtagg cggccaggga tccgagcgat atgagggtgt ccatgttggc gctcatgtgg
     2461 agagcctgac ggaacgcaac tcgatggaac tgccagccgc accagaacac caccggggtg
     2521 accagggccc cttgggccag ccggctccat tgagcctccg gcccgagcat ggcgaggagc
     2581 acggcaggga gcgtcagcgc ggcggcaacc caaaaccggc gccactgctt ctcctcgtcc
     2641 gagtggtaat gctcgaccat gtcgcgctgc ggggcgccgc tgacacgacg agtgatggtg
     2701 taaccgatct tggccaccgc ctcactaaga gccccgacgt ctgtgccgtc gtggacgcga
     2761 acgagcgcgg aggcgccggc caggttgacg ctggctgcct ccaccgcctc ttgtcggagc
     2821 aggactcgtt cgatgcgcac cgcgcaggtg gcgcacgtca tgccgcccac gtcgaacagg
     2881 atggtttcgg cggtcgcggc cgggccggtc tcagccgaat acggcgtagc cctgctcctc
     2941 gatggtcttc ttgatcaagg cgagctcgac tcgggcgtcg tcgtaggcaa ccctcaccgt
     3001 ggcgtcggcg atggccacct ccaccgactg cacaccatcg agcgtcccaa ctgcgtcctc
     3061 gatgctcatc ttgcagtgat cacagtggat ctcgggtgcc tgaaggagaa cctcgctcat
     3121 gacgtgaggg tacccgggac ccaacactca gaacgcagaa acacaaacga gagccccggt
     3181 ccatggaccg gggctcttgg tctgactcgg gacggggagg gggagaggac ctgtcccggc
     3241 cagagtcttc aggcgtcttg tccgtccatg atcttgccgc ggacccgggg gaatcggacc
     3301 ggggcatgag catggcaata ctccgccttg ttgtactgag agagtttggt ggtgcagccc
     3361 ttcttggcgc agacgcggcc ctcgttgaat ctcttcgaag gtcgggtcat gcccttgatg
     3421 gctgcccctc taacgatgtc tgtcatttcc gtggccctcc ttgacctggg ccggtgatta
     3481 gttcaagtct cttttgttgt ctcgtgtgtt tgccggtacg agaatccttc aggattcgcg
     3541 tgcgcactac cactgtgcga gccagccctt aaagcggcaa taaacaaccg ctcctgggaa
     3601 tgagacgtcg ggaagcctga atcggttccc gcgatgtcac actctacccc ggtgacaccg
     3661 tcgatcacca gaccagcctg ccggcgatac acagatgatc atcggcatgc cgggtagggg
     3721 acctgagcgt cactcgatgc cgagctttct caggaaggcg gtgttgtcgg gctcacgacc
     3781 gaggaaggcg gtgagcatct cgtctgcatc caccgatcca cctttctcca acacttcgcg
     3841 gcgataggcc attccaactt cagggttggt gacgccttgc tcctcgaacc gcgagaacat
     3901 gtcgtccccg aacacctcgg accacatata cccgtagtaa gcggcgtcgt aaccacccat
     3961 gagatggccg aacgacgcga gggcgaaggt gccctcatgg aaggggatca ggccaagccg
     4021 ggccccttcg cggaggacga cgtcgaagtc gatctgggga tcagagccgt gcatcgtttg
     4081 atcccaccat ccgtactgaa gttgacggag ctggtggatg gacacgttga gctggcgggc
     4141 agcgacgagt tgctcgacca gctgatcggg gatcgagtcg cccgtctcgt ggtgccgggc
     4201 gaaacgacgg aggacgtcgg cacgccacac ccaatgctgc atgatctggc tcggcgcctc
     4261 gacgaagtct ctctcgacgc tggtcccggc gaaccgagcc agctcggtcc tgcccaggtt
     4321 ctgatgaaga acatggccga actcgtggaa gagtgtctcg acctcg
//
LOCUS       DASTXO010000015         3096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1069138, whole genome shotgun sequence.
ACCESSION   DASTXO010000015 DASTXO010000000
VERSION     DASTXO010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069138"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /gene="tsaD"
                     /locus_tag="VFU96_00260"
     CDS             <1..829
                     /gene="tsaD"
                     /locus_tag="VFU96_00260"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU5111714.1"
                     /translation="LNEAGIHPADLDGVGATAGPGLVGALLVGHSFAKATAWARQLPF
                     VGIDHMEGHLMAPLLQFEEFRPPVVSLLASGGHSQIVHVAEWGEYRVMGSTIDDAAGE
                     AFDKLARVMGLGFPGGPAIDAASEGGDPAAVDFPRALPDRRFDFSFSGLKTSVVTYID
                     KARAGGELPPLADVAASVQEAIVDVLVDKTFNAVDETGARIVGGGGGVLANRRLRHRF
                     AEEADKREVILAIPEPILCTDNAAMIGVAAAHWLSTGRITDWAENVDPGLVLGHPDL"
     gene            1000..1290
                     /gene="groES"
                     /locus_tag="VFU96_00265"
     CDS             1000..1290
                     /gene="groES"
                     /locus_tag="VFU96_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU5111715.1"
                     /translation="MKLQPLGDRVVVRPTEDMESRTPSGLVIPDTAKEKPQIGEVIAV
                     GPGARDDDGDRIPMDVSVGDKVLYSKFAGTEVKLDSDEYLVLSERDLLAVVN"
     gene            1305..2942
                     /gene="groL"
                     /locus_tag="VFU96_00270"
     CDS             1305..2942
                     /gene="groL"
                     /locus_tag="VFU96_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006133870.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU5111716.1"
                     /translation="MAAKELRFNEEARKGLQAGVDKLADTVKVTLGPKGRNVVLEKKW
                     GSPTITNDGVTIAKEIELDDPYENMGAKLAYEVANKTDNVAGDGTTTATVLAQAMVRE
                     GLRQVAAGANPMELKRGIEQAVETAVKSIGEQARDIETSEEIAHVASISANNDSSIGQ
                     TIADAMDRVGKDGVITVEEGQTFGLELEFTEGMQFDKGFINFYFITDADRQEAVLEDP
                     YILIANQKISSVNDLVPVLEKVMQSGKSVLVIAEDVEGEALATLVVNKIRGTFSSVAV
                     KAPGFGERRKAMLQDIAILTGGTVISEEVGLKLENVTLDMLGRARKVVVAKDNTTVID
                     GSGTEADVKARIKQIEREIENTDSDWDKEKLQERLAKLSGGVVVIKAGAATEVELKEK
                     KHRIEDAVQAARAAVEEGVVPGGGVALLRAQTAVDKVRGGTDDEKAGRSIVRKAMEEP
                     LRQIAVNAGFEGGVVVERVRAASGSEGFNAATGEYGDLVADGVIDPAKVTRSTLQNAA
                     SIAALLLTTEALVAEEKEPAPAMPGGGHGHDGGGMDF"
BASE COUNT          581 a          948 c         1042 g          525 t
ORIGIN      
        1 cctcaacgaa gcagggatcc atcccgccga cctcgatggg gtcggcgcca ctgccggtcc
       61 cgggttggtc ggcgcccttc tcgttggcca ttccttcgcc aaggccactg catgggcccg
      121 ccagctcccg ttcgtcggca tcgaccatat ggaagggcac ctgatggcgc cgttgctcca
      181 attcgaggag ttccgcccgc ccgtggtgtc gctgctcgcc tcgggcggtc actcccagat
      241 cgttcacgtc gctgaatggg gggagtaccg ggtcatgggc tcgacgatcg atgacgcggc
      301 cggggaggca ttcgacaagc tggcccgggt gatgggcctc ggattcccgg gcggtcccgc
      361 catcgatgct gcatccgagg gtggggaccc ggcagctgtc gatttcccca gggccctacc
      421 cgatcgtcgc ttcgatttct cgttctcggg gttgaagacc tcggtggtga cctacatcga
      481 caaggcgagg gcgggtggcg aattgcctcc cctggccgat gtcgccgcct cggtccaaga
      541 ggcgatcgtc gacgtgctcg tggacaagac tttcaacgcg gtggacgaga ccggtgcccg
      601 gatcgtcggc ggtgggggcg gggtgctggc caaccggcga ctgcgccacc gcttcgccga
      661 ggaggcggac aagcgcgagg tgattctggc gatcccggag ccgattctct gcaccgacaa
      721 tgccgccatg atcggtgtcg ccgctgccca ttggctgtcc accggccgga tcaccgactg
      781 ggccgagaac gtggatccgg gtctggtcct cggtcacccg gatctctgag gtacggtgga
      841 cctgagtcac ctcgactcag gtttcgatta gcagtcagtg gtggcgactg ctagtgttct
      901 tctagcactc aaccgatacg agtgctaatg cttgggtgtg cgccatccgg tgggcactca
      961 ggcgcaggca ctaaccgagg ttcaaggagg agccagagta tgaagcttca gccgctcggt
     1021 gatcgagtgg tggtaaggcc aaccgaggat atggagtcac gcactccgtc cggtcttgtc
     1081 atccccgaca ccgccaagga gaagccgcag ataggcgaag tcatcgctgt tgggcccggc
     1141 gctcgggacg atgacgggga ccggatcccg atggacgtgt ccgttggcga caaggtcctc
     1201 tattcgaagt tcgccggaac cgaggtcaag ctcgactcgg acgaatatct agtcctgtcc
     1261 gagcgcgacc ttctagccgt cgtcaactga ggagctgata accaatggca gcgaaggaac
     1321 ttcgcttcaa tgaggaggcc cgcaaggggc tccaggcggg cgtagacaag ctcgccgaca
     1381 cggtaaaggt gacgctcggt cccaagggcc gcaacgtcgt cctcgagaag aagtggggct
     1441 cacccacgat caccaatgac ggtgtgacca tcgccaagga gatcgagctc gatgacccct
     1501 acgagaacat gggcgccaag ctcgcctacg aggtagccaa caagaccgac aacgtcgccg
     1561 gcgacgggac caccacggcc accgtgctgg cccaggcgat ggtgcgggaa ggccttcgtc
     1621 aggtggctgc aggcgccaac ccgatggagc tcaagcgtgg catcgagcag gccgtcgaga
     1681 ccgccgtgaa gtcaatcggc gagcaggctc gtgacatcga gaccagcgag gagatcgccc
     1741 acgtggcgtc gatctcggcc aacaacgact cctcgatcgg ccagaccatc gcagacgcga
     1801 tggaccgagt cggcaaggac ggtgttatca ccgtcgaaga gggtcagacg ttcggactcg
     1861 agctcgagtt cacggagggg atgcagttcg acaagggctt catcaacttc tacttcatca
     1921 cagacgccga tcggcaggag gctgtgctcg aggatcccta catcctcatc gccaaccaga
     1981 agatcagctc ggtcaacgat ctcgtacccg tgctcgagaa ggtcatgcag tccggcaaga
     2041 gcgtgctggt gatcgccgag gatgtcgagg gtgaggccct cgccaccctg gtggtcaaca
     2101 agatccgcgg gacgttctcc tcggtcgccg tcaaggcgcc cggtttcggc gagcgtcgca
     2161 aggcaatgct ccaggacatc gccatcctca ccggtggcac cgtgatctcc gaggaagtgg
     2221 gtctcaagtt ggagaacgtc actctcgaca tgctgggccg agcccgcaag gtcgtcgtgg
     2281 cgaaggacaa caccaccgtc atcgatggct cgggtacaga ggccgatgtc aaggctcgga
     2341 tcaagcagat cgagcgtgag atcgagaaca ccgactccga ttgggacaag gagaagctcc
     2401 aggagcgtct cgccaagctg tccggcggtg tcgtggtgat caaggccggc gcggccaccg
     2461 aggtcgagct caaggaaaag aagcaccgca tcgaggatgc agtccaggcg gcgcgtgctg
     2521 ccgtcgagga gggcgttgtc cccggtggtg gtgtggccct gctccgtgct cagaccgccg
     2581 tcgacaaggt tcgtggtggg accgacgacg agaaggccgg tcgttccatc gtccgcaagg
     2641 cgatggagga gccactccgt cagatcgcgg tgaacgccgg cttcgaaggt ggagtcgtcg
     2701 tcgagcgtgt tcgcgccgct tccggcagcg aggggttcaa cgcggcaact ggcgagtacg
     2761 gcgacctggt cgccgacggg gtaatcgacc ccgccaaggt cactcgctcg accctgcaga
     2821 acgcggcgtc gatcgcggcc ttgctcctga ccaccgaggc cctcgtggcc gaggagaagg
     2881 agcccgcccc ggcgatgccg ggtggcggcc acggccacga cggcggtggg atggacttct
     2941 aagtccgacc aacccaattc gagaaggggt cccgagaggg gccccttcgc tcatctcagg
     3001 tgagcggcag tccccccgcg aggcggtcca gatagttggt ggtctcgtct cggtccagcg
     3061 agcggacgtt gaagatggtg tggtcgacgc cggcct
//
LOCUS       DASTXO010000016         3114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1658643, whole genome shotgun sequence.
ACCESSION   DASTXO010000016 DASTXO010000000
VERSION     DASTXO010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1658643"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..858)
                     /locus_tag="VFU96_00275"
     CDS             complement(103..858)
                     /locus_tag="VFU96_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111717.1"
                     /translation="MHEHPLDLIAALADGSIGDETEARALIESCQVCRDEYVAQTEVI
                     EWLTTVPTIEMTELEKATLHRDLWTELRNQPAKRSAMPWWQRLSYVAAGLLVALGLVG
                     VLNSGLLGGGADSGQTLVAEALDEGPTEEVPFDAQGSDDGESQPEMTTATTAAATATT
                     TAGAEAGGDLLSVPFEELADEARARQSDRVTTYSTDADFAECLDRVGLTDHVVVDEID
                     LDQTYLLVMRRDPEAEPIVTFVLVPACEIVYVG"
     gene            complement(877..1422)
                     /locus_tag="VFU96_00280"
     CDS             complement(877..1422)
                     /locus_tag="VFU96_00280"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5111718.1"
                     /translation="MTVPGNEAELLTRVVSGDREAFDAVMRNHQDRVFSVCFRILGDR
                     ERALDATQDTFLTVFRKAGQFQGRSAVGTWIYRIAVNTCYDQLRRAQRRPSEALPDVD
                     PADPAAEEAMESAALRPEIEVALAQLPPDFRNAVVLSDLEGMSLPEVAEILGVPIGTV
                     KSRVFRGRRLLAKHLGNQIAQ"
     gene            complement(1419..2714)
                     /locus_tag="VFU96_00285"
     CDS             complement(1419..2714)
                     /locus_tag="VFU96_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111719.1"
                     /translation="MPPPVSDRYRLEMRLGRDNDIEEWLATDTSLERPVLIRSLGPES
                     SQERRDRFVASVSGAAKTGHPHLSRVFAVEDVAGGAYAVCEWTGGASAADRLAADQTV
                     GLEEFLPNASGLAGALGALHESGGVHGQIDLSAISWSVAHPAKLGGFGRERRTDQDGD
                     VRALAAALETALTGSAPGGPPPSESIDGVPRAIDRVLRSAQSGRLTASDLEKALLASP
                     TLRPAQPEPLSTSRRLLYVAVGLVVLAVGLVALGRLFIGGGQIVPISPTTTVGVDNSG
                     TTTSTSAGTTLAPGPVSVVDFGSFDPFGGGGENDEALGNVLDGSASTTWRSERYQDPL
                     SLVKPGVGLRFQVSGAPSAVQLVGLTSGTEFSIFWAQEPPDQIEGWERALTATAESST
                     TLFPLPRREGGHWLLWMTDFPEQSDGTYYAELGEVRFTP"
     gene            complement(2768..>3114)
                     /locus_tag="VFU96_00290"
     CDS             complement(2768..>3114)
                     /locus_tag="VFU96_00290"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5111720.1"
                     /translation="WRMTEAGLVEAMRATPRSSVLTALIDDRLAGYALVGSQMTISFL
                     QRVAVAPSFGGQGVGRSLVRASAVWASKRGARALVLNVRPENDRARRLYEREGFQDRE
                     TQLHLLRYEHVA"
BASE COUNT          530 a         1027 c         1017 g          540 t
ORIGIN      
        1 cagcgaacgg accttgatcg agatctgctc gaggatctcg gcaaaccggg ctgctaattc
       61 ctccattgat gccgagatta gttaccccga ggtctccgtc ggctaaccga cgtacacgat
      121 ctcgcaggca ggtactagga cgaaggtgac gatgggctcc gcctcgggat cccttcgcat
      181 gaccaggaga taggtctgat cgagatcgat ctcatcgacg acgacgtggt cggtgagacc
      241 gacgcggtcg aggcattcag cgaaatcggc gtcggtgcta taggtggtga cacgatcgct
      301 ctgccgggcc cgggcctcat ctgccagctc ctcgaacggc accgagagaa ggtccccccc
      361 tgcttccgcg ccggcggtgg tcgtcgccgt ggcggcggcc gtggtcgcag tggtcatctc
      421 gggctgggac tccccgtcgt cactgccctg tgcatcgaag gggacctcct cggtcgggcc
      481 ttcgtcaaga gcttcagcga cgagggtttg cccggagtcc gctccgccgc cgagcaggcc
      541 gctgttgagg acgccgacca accccaaggc gaccaacaag ccggccgcaa cgtaggagag
      601 gcgctgccac cacggcatcg ccgatcgctt cgcaggctgg tttcgcagct cggtccagag
      661 gtcgcgatga agggtggcct tctccagttc ggtcatctcg atggttggaa cagtggtcag
      721 ccattcgatg acctcggtct gggcgacgta ctcgtcacgg cagacctggc aagactcgat
      781 cagcgcgcgc gcctctgtct catcgccgat ggagccatca gcgagggcag cgatcagatc
      841 caggggatgt tcatgcattc gttctcttgg acggtctcat tgggcgattt ggttccccag
      901 gtgcttggcg aggaggcgtc ggccccggaa cacccgtgac ttcactgtcc cgatcggcac
      961 cccgaggatt tctgctacct cggggagtga catcccttcg agatcggaga gaaccaccgc
     1021 atttcgaaag tccgggggca gttgcgccag ggcgacctcg atctcgggtc ggagtgcggc
     1081 cgattccatc gcctcctcgg ccgcaggatc cgccggatcc acgtcgggga gggcctcgga
     1141 tggtcttcgc tgggcccgcc gcagctggtc gtaacacgtg ttgaccgcga tccggtagat
     1201 ccacgtgccg accgcggatc ggccctggaa ctggccggct ttgcggaaca cagtgaggaa
     1261 cgtgtcctgg gtcgcgtcga gagctctctc tcgatcaccg aggatgcgaa agcacaccga
     1321 gaacacgcgg tcctggtggt tgcgcatgac tgcgtcgaag gcctcccggt ctccggagac
     1381 gacccgagtc agtagctcgg cttcgttgcc ggggaccgtc acggcgtgaa tcgtacttct
     1441 cccaattcgg cgtaataggt tccgtcggac tgctctggga agtccgtcat ccacaacagc
     1501 cagtgcccgc cctcacgtcg ggggaggggg aaaagcgtgg tgctcgactc cgcggtcgcg
     1561 gtgagagcgc gctcccaccc ttcgatctga tcaggtggct cctgggccca gaagatgctg
     1621 aactccgttc cactcgtgag accgacgagt tgcactgccg acggcgcgcc gctcacctgg
     1681 aagcggagcc cgacaccggg cttcaccagc gacagggggt cctggtatct ctccgaccgc
     1741 catgtggtcg aggcgctccc gtcgagcacg tttcccaggg cttcgtcgtt ctcgccgcct
     1801 ccaccgaacg ggtcgaacga tccgaagtcg accacgctca ccggcccagg tgcgagcgtc
     1861 gtgcccgcac tggtgctggt ggtcgtgccg gaattgtcga ctcccacagt ggtcgtcggg
     1921 gagatcggaa cgatctgacc tccaccgatg aagagccgtc ccagcgccac gaggcccacg
     1981 gcgagcacca cgaggccgac ggcgacatag aggagacgac gagatgtgga cagaggctcc
     2041 ggttgcgccg ggcgcaaggt gggtgaggcg agcagggcct tttccaggtc agaagcggtt
     2101 agacggccgg actgcgccga gcgaagcacc cggtcgatgg ctcggggaac cccatcgatg
     2161 ctctccgagg gaggcggccc acccggggcc gacccggtga gagcggtttc cagggcggcg
     2221 gcgagcgccc tcacgtctcc atcctgatca gtgcgtcgct ctcgaccgaa ccctcccaac
     2281 ttggcgggat gcgcgaccga ccatgagatg gccgagaggt cgatctggcc gtggacgccg
     2341 cccgactcgt gcagcgcgcc gagcgctccg gctagacccg acgcattcgg gaggaactcc
     2401 tcgagcccga ccgtttgatc cgcggccaac cggtctgcgg ccgatgcccc tcctgtccac
     2461 tcacaaaccg catacgcccc accggcgacg tcttcgaccg cgaacacacg ggaaagatgg
     2521 ggatgccccg tcttggcggc tccgctcaca ctcgccacaa atcggtcccg ccgctcttga
     2581 ctcgattccg gcccgagtga ccgaatcagg accggacgct cgagcgaggt gtcggtggcc
     2641 agccattcct cgatgtcgtt gtctcgcccg agtcgcatct cgaggcggta gcgatcagag
     2701 accggcggtg gcatcttcta ttgccggccg acgttggccg actggctcct ggtcatgggt
     2761 tcatctgtca tgcgacatgc tcgtatcgca gcagatgaag ttgcgtctct cggtcctgaa
     2821 acccttcgcg ttcgtagaga cgccgggccc gatcgttctc ggggcgaaca ttgagcacca
     2881 gcgcccgcgc cccgcgcttc gaggcccata ccgctgacgc ccgcaccagt gagcgcccga
     2941 cgccctgtcc gccgaacgag ggggcaacgg cgactcgctg caggaacgag atcgtcatct
     3001 gggatcccac cagcgcgtag ccggcgagcc ggtcgtcgat cagggcggtg aggaccgagg
     3061 agcgtggggt ggctcgcatc gcctcgacca ggccggcctc ggtcatccgc caga
//
LOCUS       DASTXO010000017         3167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1253366, whole genome shotgun sequence.
ACCESSION   DASTXO010000017 DASTXO010000000
VERSION     DASTXO010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253366"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VFU96_00295"
     CDS             <1..336
                     /locus_tag="VFU96_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111721.1"
                     /translation="VGVVLLDVPAPDATLSVALIPEIAWDHPTNLEHLDIVPEFEGRF
                     ANEPVSFPSPLAVVTATQGASSPEDQAKWLEASPDAHQIELDGGHEIYLDDPDGAASE
                     VLAFVQAQN"
     gene            complement(355..1893)
                     /locus_tag="VFU96_00300"
     CDS             complement(355..1893)
                     /locus_tag="VFU96_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111722.1"
                     /translation="MGKRLLVVGLIASVLVVTRAGEASACSCASITPAVALEETDLAF
                     VGTLVDRPQSEMPYVADAPYSFEVEAWVKGDLGSEVVVHAPNQGSACGIEAPVGERVG
                     LFVNEENGQLTSSLCSMTDPDLLLAGDSPLAIDGTGPPVFLVAGYDGPARLMLIDAAG
                     GLVAAIGAEGESMNGLAMCNGSDRFVELMNDQLVVRSTVDLGEFRRVDLDNLPYERSV
                     PKIWCRDESGDTIWLSVDQWSESDGPMIQLVDADDIGHPILEGAYGWLEVGNWAAVGG
                     EGPTARTIWRIDLQTKDRVLLHEIPMEPGDSEPFGAAWVDPAGDRVMITQWRYRDGSG
                     GNTTFFLYDLGSGDLLWQSEVMPTADGTGWADDHTFAAFSYPDVNSDVADNLLIDTET
                     ESIAHLPDSPGWRVLRVGEDLRGVMGAHLRVMPIAGGPVGELRLLPSEIHQLAAVLQP
                     EADTTSATVVAQMASPADPEGTSVSQDAIESMPWPGIAVAAGLLGAAWSGLVIRRRHR
                     SNRA"
     gene            complement(1943..2515)
                     /locus_tag="VFU96_00305"
     CDS             complement(1943..2515)
                     /locus_tag="VFU96_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012998338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5111723.1"
                     /translation="MASKPAVPDYELVDTIELTEPTQVQAIGNQLRTTILGLLHERAA
                     TVTEIAGAVGRPKSTVAYHVEVLLGAGLVRVVRTRRVRAIEERFYGRTARMFYVGEGK
                     SASDTVMPLDFNDFEVAAQESQLAYEAHRLWSFIRHARLPADRAAEFWTEVMTLVQGF
                     DRLPRSGDTAYGFVVGLYPIPDYPMLPEPD"
     gene            2579..3157
                     /locus_tag="VFU96_00310"
     CDS             2579..3157
                     /locus_tag="VFU96_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU5111724.1"
                     /translation="MAEIVLFHHVLGLTPGIRAFADELGAAGNVVHTPDLFDGHTFPT
                     LDEGMAYARSVGFEPILDRGVFEAESLRLELVYAGFSMGVMPAQKLAQTRKGARGALF
                     FDSCLPVTEFGSEWPAGVPVQVHGMEADPIFAGEGDLDAARDLVDSTDQAELFLYPGD
                     VHVFADSSLPWYDEAAADLMTSRVLGFLSMID"
BASE COUNT          571 a         1044 c         1001 g          551 t
ORIGIN      
        1 gtgggagtgg tactgctcga cgttccggcg ccggacgcca ccctttcggt tgccctgatc
       61 cccgaaatcg cgtgggacca cccgacaaac ctggagcacc tggacatcgt tccggagttc
      121 gagggtcggt tcgccaacga accagtctcg ttcccctcac ccctcgccgt ggtcaccgca
      181 acccagggtg cttcgagccc cgaggaccag gcgaaatggc tggaggccag cccagacgcc
      241 caccagatag aactcgacgg gggacatgag atctacctcg acgaccccga tggcgcagct
      301 tccgaggttc tcgccttcgt gcaggcccag aattgatcgg actagcccga taggtcaggc
      361 tcgattcgag cggtgccggc gtctgatcac gagaccagac cacgccgctc cgagtaggcc
      421 ggccgccacg gcaatccctg gccagggcat cgactcgatc gcatcttgcg aaacactggt
      481 gccttccgga tccgcaggtg aagccatctg ggcaacaact gttgcactcg ttgtgtcggc
      541 ctccggttgg agcaccgccg ccagttggtg tatctcggag ggaaggagcc gaagctcgcc
      601 gaccgggcca ccagcgatcg gcatgacccg gagatgggcg cccatgaccc ccctgagatc
      661 ctcgcccacc cgcagcacac gccatccggg agagtcgggg agatgtgcga tgctctcggt
      721 ttcggtatcg atgagaagat tgtccgcgac atcgctgttc acgtctggat aggagaatgc
      781 ggcgaaggtg tggtcgtcgg cccaaccggt gccatcggcg gtcggcatca cctcgctttg
      841 ccaaagcagg tcaccactgc ccaggtcgta caggaagaat gtcgtgttgc ccccagagcc
      901 atcgcggtag cgccactggg tgatcatgac tcggtcgccc gcagggtcga cccaggccgc
      961 tccgaagggc tccgaatcgc ccggctccat cgggatctca tggagcagaa cgcggtcctt
     1021 ggtctgcagg tcgatgcgcc agatggtcct ggcagtcggg ccttctccac ccaccgcagc
     1081 ccagttgcct acctcgagcc acccataggc tccctcgaga atgggatgac cgatgtcgtc
     1141 agcatcgacc agctgaatca tcggcccgtc gctctcggac cattggtcga ccgacagcca
     1201 gatcgtgtca cccgattcat cccggcacca gatcttcggc acggatcgct cgtaggggag
     1261 gttgtcgaga tcgacccggc ggaactcgcc aaggtcgacc gtcgatctca ccaccagctg
     1321 gtcgttcatc agctccacga accgatcgga gccgttgcac atcgccaagc cgttcatgga
     1381 ctcaccctcg gcgccgatcg ccgccaccag gccgccggcc gcgtcgatca gcatcagccg
     1441 ggcggggccg tcgtaaccgg cgacgaggaa gaccgggggc ccggtgccgt cgatggccag
     1501 cggactgtcc ccggcaagca acaggtcggg atcggtcatg gagcacaggc tgctggtcag
     1561 ttggccgttc tcctcgttta cgaagagacc cacacgctcg ccgacgggag cctcgatccc
     1621 gcaggccgag ccctgattgg gagcatgaac caccacctcc gagcccaggt cgcccttcac
     1681 ccatgcctcg acctcgaagc tgtagggcgc atccgcgacg tatggcatct cagattgcgg
     1741 ccgatcgacc agggtcccga cgaaggcaag atccgtctct tccagggcca cggccggagt
     1801 gatcgatgcg cacgagcacg cggaggcctc accggcgcgc gtcaccacca gcaccgaggc
     1861 gatcagaccg acgaccagca gacgtttgcc catcacgtcc ctttgacggc gggtttggcc
     1921 ggaaggttcc agctgatgac ggtcaatccg gctcaggcag catcgggtaa tccggaatcg
     1981 ggtagagccc gacgacgaac ccgtaggcgg tgtctcccga tcggggaagc cggtcgaatc
     2041 cctgcaccaa cgtcatgacc tcggtccaaa attcggcggc gcggtcggcg gggagccggg
     2101 cgtgtcggat gaacgaccac agccggtggg cttcataggc caactgggac tcctgtgcgg
     2161 cgacttcgaa gtcgttgaag tccaatggca tgaccgtgtc gctggcgctc ttcccctccc
     2221 cgacgtagaa catccgggcc gtgcgcccgt agaagcgctc ctctatggct cgcacccggc
     2281 gcgtccggac cacgcggacc aggcccgccc cgaggagaac ctcgacgtga taggcgacgg
     2341 tgctcttggg gcggccgacc gcgcccgcga tctcggtcac ggtggccgcc ctctcgtgca
     2401 gcagaccgag gatcgtggtg cgcagttggt tgccgatggc ctgaacctgg gtcggctcgg
     2461 ttagctcgat ggtgtcgacc aactcgtaat cgggcactgc gggtttgctc gccatgaccg
     2521 ttcagactaa cgttcccgat tgttccaaaa ttatggatca atagacgagg aggaagacat
     2581 ggccgaaatc gtgctgtttc accacgtcct gggtctgacc cccgggatca gggccttcgc
     2641 cgacgagctg ggagccgccg gaaacgtcgt gcacaccccg gacctgttcg acggtcacac
     2701 cttcccgacg ctcgacgagg gcatggccta cgccagatcg gtcggatttg agcccatcct
     2761 cgaccgaggg gtcttcgagg ccgagtcgtt gcgactcgag ctggtctatg cgggattctc
     2821 gatgggcgtt atgcccgccc agaagctcgc ccaaacgagg aaaggggccc ggggggcgct
     2881 gttcttcgac tcatgtctcc cggtcacaga gttcgggagc gaatggcccg cgggggtgcc
     2941 ggttcaggtc cacgggatgg aagccgaccc gattttcgcg ggtgagggcg acctcgatgc
     3001 ggcgcgggat ctggtcgact ccaccgatca ggcagagctg ttcctctatc ccggcgacgt
     3061 gcatgtcttc gcggactcat ccctgccttg gtatgacgag gcggcagccg atctgatgac
     3121 cagtcgggtg ctcggcttcc tatcgatgat tgactgagcg cgagcct
//
LOCUS       DASTXO010000018         3481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_406294, whole genome shotgun sequence.
ACCESSION   DASTXO010000018 DASTXO010000000
VERSION     DASTXO010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406294"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /locus_tag="VFU96_00315"
     CDS             complement(<1..1472)
                     /locus_tag="VFU96_00315"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5111725.1"
                     /translation="MIKRFTGKFGASSLGARVATLLAQLRADISFAFVDAMVIAVAYL
                     SALGVLSLDGRLDGVWWTRVAVALPVIIGVHLVANVAMGAYGHVWEFASISEARRVIA
                     ATFWASAVLILVVLAMGDLELIEGPGNRAVPLSVLVLGSMISLGFLGAVRFRSRLFSF
                     HRGLRLDARDRVLILGTSKRAADLARYTGPYEVDVVGFVSSGPTPKGRRLANLPIVGE
                     LEDIASLVTKLNVQQVIVASEGASVLARRLVDLCVDIDVRLRIVPDLDELLTGYDGGP
                     DIRDLEIEDLLLRPAVSTDLEAVESILYDKTVLVTGAGGSIGSEIVSQALAFMPARVV
                     AMDNDETHLHDNMVKWDAPAGTKLTPVLCDVRDKRKLKALIEKYQPQVIYHAAAHKHV
                     PILEDWPEEAVKTNVVGTSNLLKVVRDTGVERFVLISTDKTVEPKGVMGASKRMAEML
                     VQSASATETDGCIYSSVRFGNVLGSRGSVVPTFMEQIRKGG"
     gene            1815..2693
                     /locus_tag="VFU96_00320"
     CDS             1815..2693
                     /locus_tag="VFU96_00320"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU5111726.1"
                     /translation="MKALVTGSAGFIGKHVSRALLANGFEVTGYDQKESTEPGVESVV
                     GDFLDLEALSAAVEGHDVIVHIGAIGDVYLAATNPELAASVNVTGSTNIALAAEATGA
                     RVVYASTWEVYGEPVYEPVDEKHPCEPDHPYNITKLAGERMLIAADRLRDVPVVALRL
                     GTAYGLGMRPNSVFEIFIDKARKGEPITIQGDGSQGRQFTHARDIAQAFVAAAKSDVR
                     GIALNVVSPEMISIKQLADVVTERFPTEVTYGQPRPGDVAPSYVSAARIEEKLGWKTE
                     VSFEEGMAELLSVDRG"
     gene            2695..>3481
                     /locus_tag="VFU96_00325"
     CDS             2695..>3481
                     /locus_tag="VFU96_00325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5111727.1"
                     /translation="MGSEKIHVLVLIKGLGIGGAEKLISEGARFWDRDSFHYTVAYVL
                     PWKDQLVGDLEDQGIEVHMIGGERGLTVPTVGRLRRLVKDLDIDLIHAHLPTMGIVAR
                     IASPVPVIYTEHNMADSYRPITRTMNRLTYGRNAAVTAVSGAVAGSVSTWPGEPPIVI
                     PNGVAVHVDSEQILAARQELRISDSDPLVAHVGNIRPGKGHDVLIDAVGHLHERMPSV
                     RFASIGGEKYPGDLDRVRNRAIEAGLEDCLRFMGRRTDALSFVA"
BASE COUNT          647 a         1110 c         1108 g          616 t
ORIGIN      
        1 cctcccttgc ggatctgctc catgaaggtg gggaccacac tcccgcggct gccgaggaca
       61 ttcccaaatc gaaccgacga gtagatgcag ccgtcggtct cggtggcgga ggctgattgc
      121 accagcatct cggccatccg cttcgaggcg cccatgacgc cctttggctc gaccgtcttg
      181 tcggtggaga tcaggacgaa acgctcgaca ccggtgtcac gaaccacctt gagcaggttg
      241 ctggtgccga cgacgttcgt cttgaccgct tcctctggcc agtcctcgag gatgggaacg
      301 tgcttgtggg cagcggcgtg atagatgacc tgcggttggt acttctcgat cagtgccttg
      361 agcttcctct tgtcccggac atcgcacagg actggggtca gcttggtgcc tgcgggcgcg
      421 tcccacttga ccatgttgtc gtggaggtgg gtctcgtcgt tgtccatcgc cacgacccga
      481 gccggcatga aggcgagcgc ctggctcacg atctcggacc cgatcgagcc cccagccccg
      541 gtgaccaaga cggtcttgtc gtacaggatc gactcgactg cctcgaggtc ggtgctgacc
      601 gcggggcgga ggaggaggtc ttcgatctcg aggtccctga tgtcgggccc gccgtcgtag
      661 ccggtgagca gctcatcgag atcggggaca atcctcagcc ggacatcgat atcgacgcag
      721 aggtcgacca gtcgccgggc gaggacgctg gcgccctccg aggcaacgat cacttgctgg
      781 acgttcagct tggtcacgag cgaagcgatg tcttcgagct ccccaacgat cggcaggttg
      841 gcgagacgtc ttcccttcgg ggtgggcccg gagctgacga acccgacgac atcgacttcg
      901 taggggccgg tgtagcgggc gaggtcagcc gcgcgcttgc tggtgccgag gatgaggacg
      961 cgatcgcggg cgtcgaggcg gaggccgcgg tggaaggaaa agagacggga gcggaagcgg
     1021 accgccccga ggaagccgag cgagatcatc gacccgagaa ccagcaccga gaggggaacc
     1081 gcacggttgc ccggcccttc gatcagctcc aggtcgccca tggcgaggac gaccaggatc
     1141 aggactgctg aggcccagaa ggtggcggcg atgacccggc gcgcctcgct gatgcttgcg
     1201 aactcccaga catggccgta tgcacccatc gcgacgttcg ctacgagatg gactccgatg
     1261 atgaccggca gcgccacggc gacccgggtc caccagaccc cgtcgagccg gccgtccagg
     1321 gagagcactc cgagcgccga gaggtaagcg acggcgatga ccatcgcatc cacgaacgcg
     1381 aacgagatgt cggcacgaag ctgggcgagc agtgtcgcca cccgtgcccc caacgaacta
     1441 gccccaaact tcccggtgaa ccgcttgatc acggcatccg ccctcttttt gtcccagtcg
     1501 gaagtgcgcg gcggcgtcta gcccccaggc agcatccgta atgcctccca aaatccagtc
     1561 agggaccgaa atcgaacgaa agcgatctgt cccccgaccc agccgatgcg ttacgagacg
     1621 cctcggcccg ttgcgttacc ttaaccactc tgcgtgctgg ttaccagcac gtaattcgat
     1681 ttttttcaga ttctcgattt cgcacgatgc ggagtggcct gggaggacat ggaaggaccc
     1741 ctgctattga gaggtgatca ccctccgtgt tattcttccc gacctcagtg gggtattcag
     1801 agggaggact caccatgaaa gcattggtga ctgggtctgc aggcttcatc ggcaagcatg
     1861 tctccagagc attgttggcc aacggattcg aggtaactgg ttatgaccag aaggaatcca
     1921 ccgagcccgg agtcgaatcg gtcgtcggcg attttctcga tctcgaggca ttgagcgccg
     1981 cggtcgaggg ccatgacgtc atcgtccaca tcggcgccat cggcgacgtg tacctcgccg
     2041 ccaccaaccc ggagctcgct gcttcggtca acgtcaccgg gagcaccaac atcgctctcg
     2101 ccgctgaggc gactggggcc agagtggtct acgcctcgac atgggaggtc tacggggagc
     2161 ccgtttacga gccggtcgac gagaagcatc cctgcgagcc cgaccacccg tacaacatca
     2221 ccaagctggc aggcgagcgc atgctgatcg cggccgacag acttcgtgac gtgccggtcg
     2281 tcgccctcag gctcggcacc gcctatggcc tcgggatgcg acccaactcc gtcttcgaga
     2341 tcttcatcga caaggcccgc aagggcgagc cgatcacgat ccagggtgac gggtcacaag
     2401 gtcgccagtt cacccatgcc cgagacatcg ctcaggcgtt cgtcgccgct gcgaagagcg
     2461 acgtccgagg gatcgccctc aacgtcgtgt cacccgagat gatctcgatc aagcaattgg
     2521 ccgacgtggt cactgagcgc ttccccacag aggtcaccta cgggcagccc cgacctggcg
     2581 acgtcgcccc gtcttatgtt tcggcggccc gaatcgagga gaagctcggc tggaagaccg
     2641 aagtgagctt cgaggagggc atggcggagt tgctctccgt cgatcgaggc tgaggtgggg
     2701 tcggagaaga tccacgttct ggtcctgatc aagggcctgg gaatcggcgg ggcagagaag
     2761 ctgatctcgg agggtgcccg gttctgggat cgggactctt ttcattacac ggtcgcctac
     2821 gtgctcccct ggaaggatca gctggtcggc gacctggaag atcagggcat cgaggtgcac
     2881 atgatcgggg gcgagcgggg gctcaccgtg cccacggttg ggcgcctgcg caggctggtc
     2941 aaggatctgg acatcgatct catccacgcc catctcccca ccatgggcat cgtggctcgc
     3001 atcgcctcac cggtccccgt gatctacacc gagcacaaca tggccgactc ctatcggccc
     3061 atcacccgca ccatgaatcg cctcacctac ggccgaaatg ccgcggtgac cgcggtctcg
     3121 ggggccgtgg ccgggtcggt cagcacctgg cccggcgagc ctccgatcgt catcccgaac
     3181 ggtgtcgcag tccacgtcga ctctgagcag atcttggccg ctcgtcagga gctcaggatc
     3241 tcagattccg atccgctggt cgcccatgtc ggcaatatcc gaccagggaa gggacacgac
     3301 gtcctcatcg acgccgtcgg ccatctccac gagcggatgc cgtcggtcag attcgcatcg
     3361 attggcggtg agaagtaccc cggcgatcta gatcgggttc gcaaccgcgc cattgaggcc
     3421 ggtctcgagg actgcctccg attcatgggc cgccgcaccg acgccctcag cttcgtggcg
     3481 g
//
LOCUS       DASTXO010000019         2170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_627375, whole genome shotgun sequence.
ACCESSION   DASTXO010000019 DASTXO010000000
VERSION     DASTXO010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627375"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VFU96_00330"
     CDS             <1..400
                     /locus_tag="VFU96_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HEU5111728.1"
                     /translation="VADALAAIAAELARLDGRPRPDWVVAMGGAVTNLAAVKHGLATY
                     DPDVVQGTLLDRAEIDRQIGLYRTLDAAGRRAIVGLQPARAEVILAGACIVRTIIDKL
                     GCDAVTVSDRGLRHGVLIERFGVGMDVKRV"
     gene            493..1293
                     /locus_tag="VFU96_00335"
     CDS             493..1293
                     /locus_tag="VFU96_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111729.1"
                     /translation="MPSRGTDYFADLAAQYIGATLGVKVEQWDYDGRQGVHDLQYEHE
                     GRSVAVEVKCVLDGDFRQMQGEIDRQVYQADARFRRLWIVSLDRGARIKDVRRELPDL
                     LVFLDEIGWDGQLWQLRREHPVVAARFQVLGVQAVAHLPPTETHPPGYLLLPESWGRW
                     GEDDIEEPVSFANAYLAGDNREVITLRRQLRQAQADERHVFLFIGHEHTEGWPLMKEP
                     GEGDNMALPTMTPTLPEPIDGLWLTGWSSSSRVIAWLPHNGWIEGTTG"
     gene            complement(1337..1780)
                     /locus_tag="VFU96_00340"
     CDS             complement(1337..1780)
                     /locus_tag="VFU96_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018505401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU5111730.1"
                     /translation="MASRQKPNEFSIRIRRLAVILAGMVLAVASCGDDDEGSAATTQS
                     PATSAATGGEGAGTVIEVDEKDFTIELSTMELTPGTYTFVATNNGQTTHALEIEGQGV
                     EEETEDIAPGDTAELTVTLEAGEYELYCPVGNHKDMGMKLDITVE"
     gene            complement(1887..1985)
                     /locus_tag="VFU96_00345"
                     /pseudo
     CDS             complement(1887..1985)
                     /locus_tag="VFU96_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186898.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          350 a          664 c          736 g          420 t
ORIGIN      
        1 cgtcgccgac gctttggcag ccatcgcggc ggagttggcc cgcctcgacg ggcgaccacg
       61 gccggactgg gtagtggcga tgggcggcgc cgtgacgaac ctcgcggcgg tgaaacacgg
      121 cctcgccaca tacgatccgg atgtcgtcca ggggacgctc ctcgaccggg ccgagatcga
      181 tcgacagatc gggctctacc gaactctgga cgcggccgga cggcgagcga tcgtcgggtt
      241 gcagccggcg cgtgccgagg tcatcctcgc tggcgcgtgc atcgtccgga ccatcatcga
      301 caagctcggt tgcgatgcgg tcacggtcag cgatcgcggc ctgcgccacg gcgtgctcat
      361 cgaacgcttc ggggtcggaa tggacgtgaa gagggtctga gcacggatga ggctcaacta
      421 cgaccacccg agcgaacgcc tccctgatcc tgcctgacgc ctccgacggc ggcaggtact
      481 agcctgcgcc gtgtgccttc ccgcggtacc gactacttcg cagacctggc agcccagtac
      541 atcggggcga cgctcggcgt caaggtcgag caatgggact atgacggcag gcagggtgtt
      601 cacgacttgc aatacgaaca cgaaggccgg tcggtcgcgg tcgaggtgaa gtgtgtcctc
      661 gatggggact ttcgccagat gcagggagag atcgaccggc aggtctacca ggcggacgcg
      721 cggttccgtc ggctctggat tgtcagcctc gatcgcgggg ctcgcatcaa ggacgtccgc
      781 cgagagttgc cggatctgct cgtctttctg gacgagatcg gttgggatgg ccaattgtgg
      841 cagctgcggc gagagcatcc ggttgtcgca gctcggtttc aagtgttggg cgttcaggcg
      901 gtggcacacc ttcctccgac ggagacgcat ccacctggat acctactgtt gcccgaatcg
      961 tgggggaggt ggggcgagga cgacatcgag gagcctgtca gcttcgccaa tgcgtatttg
     1021 gccggcgaca atcgcgaggt gatcacgttg cgccgtcagc ttcgtcaggc gcaggctgat
     1081 gaacgccacg tattcctgtt catcggccac gagcacaccg agggctggcc gctgatgaag
     1141 gagcctggcg agggcgacaa catggccctg cccacgatga ccccaacact ccccgagccg
     1201 atcgacggac tatggctgac gggctggtcg agctccagcc gagtcatcgc ctggcttcca
     1261 cacaacgggt ggattgaggg caccaccgga taaggcccag tccatggcct gatctccgtg
     1321 gaccgatggc gaggacctac tcgacggtga tgtccagctt catacccatg tccttgtggt
     1381 tgcccaccgg gcaatagagc tcgtactcgc ctgcttcgag tgtgacggtg agctctgcgg
     1441 tgtccccggg ggcgatgtcc tccgtttctt cctccacgcc ttggccctca atctcgaggg
     1501 cgtgggtggt ttggccgttg ttggtggcca cgaatgtgta cgtgcctggt gtgagctcca
     1561 tcgtcgagag ttcgatcgtg aaatccttct catcaacctc gatcaccgtc ccggcaccct
     1621 cgcccccggt tgcggcggat gtggctgggc tctgcgtcgt cgctgcggag ccttcgtcat
     1681 catcgccgca gcttgcgact gccagcacca tgccggcgag gattaccgca aggcgccgta
     1741 tgcggatgct gaattcgttg ggcttctgcc gacttgccac ggggtccacc tccggttgat
     1801 tccattcatt tcacgcaccg gcaccgctgt aggttcagag cccccgcgcg taggacgcta
     1861 ctagatgtac ccggccaact ggttggttac aggcggctga tcggtggcag gacgccgagt
     1921 gcggtgtggc ggcgtcgagt gttgtagagc tcgagccagg gcgcaagggc ggttgctcgg
     1981 tcgctcggtg cacgtttggg aggtgcaaat catgcgtgtc ggcctggtca cgtgcgccga
     2041 ggttgccgat ctggaacctg atgaccggct cgtcgtcggg tcactggccg cccgtggagt
     2101 gcacgccgag ccggtggtct gggatgcgca cgacgcggat tggtccgcgt acgacctcgt
     2161 cgtactgcgt
//
LOCUS       DASTXO010000020         3337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1069369, whole genome shotgun sequence.
ACCESSION   DASTXO010000020 DASTXO010000000
VERSION     DASTXO010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069369"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..900
                     /locus_tag="VFU96_00350"
     CDS             79..900
                     /locus_tag="VFU96_00350"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEU5111731.1"
                     /translation="MSGDLARALLTGADFEVIPLKGVMERALAIEPGSTVSVTASPTR
                     GMTPTVDLATELQARGYRAVPHLSARTIENRGDLSSIIKRLDHGGVGQVFITGGDGEP
                     SGAYRDAVSLLRDMAALGHPFLEVGITGYPEGHPVIPSDRLRESLLEKQVHATYIVTQ
                     MCFSPQAIIEWITSIRGLGVTLPIKVGVPGAVDLTRLLSIGARIGVGDSMRFLAKNRS
                     SILGMLRPGHYRPDRVVGPLAQIGDGLGMTGIHLFTFNQVEATVAWHRKAMSRIT"
     gene            900..1646
                     /locus_tag="VFU96_00355"
     CDS             900..1646
                     /locus_tag="VFU96_00355"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEU5111732.1"
                     /translation="MAVQSIDRAFQVLKALAVEPSGVTDLAQRVGLPKSTVARMLSSL
                     ETQGAVTRGPDGTMYSIGMGLVELAGAIDATAALATAVRPHLVWLSDQLGEAAGFSVP
                     TGYSIQYMVQVESPTPVQVRDYTGLTVPMHIGPSGLCVMAQWPAEETDRYLSRPLEAY
                     TANTVVDAAEIRKRLESIRVSGHCWVREEFAEGINSVAAPVLADGKRALGAIHVHGPA
                     YRFPAKGKADAIALLVMDAAARFSSRSDTV"
     gene            1717..2895
                     /locus_tag="VFU96_00360"
     CDS             1717..2895
                     /locus_tag="VFU96_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEU5111733.1"
                     /translation="MANERSEMLEKSTTLYFGPWYRRSPYFESTLAAGCTAYDIYNHM
                     YLPAYFDDPDVEYWALVNGVTMWDVGVERTVEVSGKDADRLIDYITCRDLTRCAVGQG
                     KYMPVIAPDGGIVNDPVLLHVDEGRWWMQLADSDAGLYALGVAAGRFDADVTLPDVHP
                     MQVQGPLAAKTMEKLVGPSIFDIKYYWLGEFVIDGIPVVVSRTGWTAIPGFEINVLDR
                     RDGPAIWDAVAAAGEEFDIRPIAPCEARRIEAGIFNYGSDMTLDDTPFHVSGMERLVE
                     PQDQDYLGKEALERVRETGVDRKLVGMFLEGDQLRAELSQPWPVLSGKEEVGRVTDAV
                     WSPGLEQNIGYMWVPLDLSTPGTRVEVVTEGGEELIAVAASLPFVDRQKKAPAQSLSV
                     "
     gene            complement(2885..>3337)
                     /locus_tag="VFU96_00365"
     CDS             complement(2885..>3337)
                     /locus_tag="VFU96_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111734.1"
                     /translation="PYLLVAAVDGVHSARIFASRSNDIEGIVLVDPMPIGFQGFYDDL
                     LPDLAGHPPWLDLDTAVSASLGDFGSVPLTVIEQDPNAVFLSSGFVEGVGRESAESVD
                     AYWQDGLAFYEGLSSSSRSVVANGTGLDMVVWDQPDLVIEQVLELLRR"
BASE COUNT          618 a         1057 c         1080 g          582 t
ORIGIN      
        1 ggacgagtcg atcgacgcgg aattggtcga gtgggtcgcc gagcgcaaag cctccttccc
       61 cgactcggac gtctgagcat gagcggcgac ctcgctcgag cgcttctcac cggggctgat
      121 ttcgaggtga tcccgttgaa aggcgtgatg gagagggcac tggcgatcga gccgggatcg
      181 actgtctcgg tcaccgcctc gccgactcgt ggaatgactc ccacggtgga tctggcgacc
      241 gagctccagg ctcggggcta tcgggccgtg cctcatttgt cggccaggac catcgagaac
      301 cgcggcgatc tctcctcgat catcaagcgg ctcgaccatg ggggagtagg acaggtcttc
      361 atcacaggcg gcgacggcga gccaagcggg gcctatagag acgccgtctc cctgctccgg
      421 gacatggccg ctctcggtca tccgtttctg gaggttggga taaccgggta tccggaaggc
      481 cacccggtca tcccatcgga ccgtctccgc gagtcattgc tcgaaaagca ggtccacgcc
      541 acgtacatcg tcacccagat gtgtttcagc ccccaggcca tcatcgagtg gatcacctcg
      601 atcaggggcc tgggggtgac tcttcccatc aaggtggggg tgcccggcgc ggtcgatctt
      661 acgcgcctcc tcagcatcgg ggctcggata ggggtgggtg actcgatgag gttcctcgcc
      721 aagaatcgaa gttcgatcct cgggatgctg cgtcccggtc attaccggcc agaccgggtc
      781 gtcggccccc tggcccagat cggcgacggt ctcggcatga ctggcatcca cttgttcacc
      841 ttcaatcagg tcgaggccac ggtcgcctgg catcgcaagg ccatgtccag gatcacttga
      901 tggcagttca gtcgatcgat cgcgcatttc aggtcctcaa ggcgctggcg gtcgagccgt
      961 ctggggtcac cgacctcgcc caacgggtcg ggttgcccaa gtcgaccgtg gcccggatgc
     1021 tttccagcct cgaaacccag ggggcggtga ccagagggcc cgatggcacg atgtactcca
     1081 tcggcatggg gctggtcgag ctcgcgggcg ccatcgatgc cacggcggca ctggccaccg
     1141 cggtccgccc tcatctggtc tggctctcag accagctcgg agaggcggcc gggttcagcg
     1201 tccccaccgg ctacagcatc caatacatgg tgcaggtgga gagcccgacc ccggtccagg
     1261 tccgtgacta cacgggtctc accgtcccga tgcacatcgg cccgtctggc ctctgcgtga
     1321 tggcgcaatg gccggccgag gagacagacc gctatctgag ccgcccgttg gaggcctaca
     1381 ccgccaacac ggtggtcgac gccgccgaga tccgaaagag gctcgagtcg atcagggtct
     1441 ccggccactg ctgggtccgt gaggaattcg ccgaagggat caattctgtg gcggcccccg
     1501 tgctggccga cgggaagcgc gctctcgggg ccatccacgt ccacggcccg gcataccggt
     1561 tcccggccaa ggggaaagcc gacgccatcg cgttgttggt gatggacgct gccgctcggt
     1621 tctccagccg ctccgacacg gtctgaccgc cgccggcacg accggcaccc gctgccctaa
     1681 ggttgaatga ccgttcagga atcgaggagg acacgggtgg ccaacgaacg cagcgagatg
     1741 ctggagaaga gcacgactct ttacttcggg ccttggtacc gacggtcgcc ctacttcgag
     1801 tccaccctcg ccgcgggctg caccgcctat gacatctaca accacatgta tctgcctgcc
     1861 tacttcgacg accccgacgt cgagtactgg gcgttggtca atggagtgac gatgtgggac
     1921 gtcggcgtgg agcgaacagt cgaggtgagc gggaaggacg cagatcggct catcgattac
     1981 atcacgtgcc gtgacctgac caggtgcgcg gtgggccagg gcaaatacat gccagtcatc
     2041 gcccctgatg gcggcatcgt caacgacccg gtcttgctgc atgtcgatga gggccgctgg
     2101 tggatgcagc tggcagattc cgatgcaggt ctctacgccc tcggcgtggc cgctggtcgc
     2161 ttcgatgccg atgtgaccct gcccgacgtg catcccatgc aagtacaagg gccgctggcc
     2221 gccaagacaa tggagaagct ggtaggcccg agcatcttcg acatcaagta ctactggctc
     2281 ggcgagttcg tgatcgatgg gatcccagtg gtggtcagcc ggacgggatg gaccgccatt
     2341 cccgggttcg agatcaatgt cctcgatcga agggatggcc ccgcgatctg ggacgcggtg
     2401 gcagccgcgg gcgaggagtt cgacatccgg cccatcgccc catgtgaggc gcgtcgcatc
     2461 gaggccggga tcttcaacta cggctcggac atgactctcg atgacacccc gttccacgtc
     2521 agcggcatgg aacgactcgt cgagccacag gaccaggatt acctgggtaa agaagcattg
     2581 gagcgcgtcc gcgagaccgg ggtcgatcgc aagctggtgg gcatgttcct cgaaggggac
     2641 cagctccgag ccgaactgtc ccagccatgg ccagtgctct cggggaaaga agaggtaggt
     2701 cgggtgaccg acgctgtctg gtcgccgggg ctggagcaga acatcggcta catgtgggtc
     2761 ccgctcgacc tctcgacacc cggtacgcgt gtcgaagtgg tgaccgaggg tggtgaggag
     2821 ctcatcgccg tcgcggccag tttgccgttc gtcgaccgcc agaaaaaggc tccggcgcag
     2881 agcctcagcg tctgagcagt tccagaacct gctcgatcac cagatcaggt tgatcccaga
     2941 ccaccatgtc gagccctgtt ccattggcga cgaccgatcg agagctcgac gacagcccct
     3001 catagaaggc gaggccatcc tgccagtagg cgtcgacgga ctccgccgac tcgcgtccaa
     3061 ccccttcgac gaacccgctc gacagaaaca ccgcattcgg gtcctgttcg atcacggtca
     3121 acggcacact gccgaagtcg ccgagagatg ccgagaccgc tgtatccaga tcgagccacg
     3181 gcggatggcc ggccagatcg gggagaaggt cgtcatagaa cccctggaat ccgatgggca
     3241 tcggatcgac aaggacgatg ccttcgatgt cgttgctccg ggacgcgaag atccgagccg
     3301 agtgcacacc gtcgactgcg gcgaccaaca gatacgg
//
LOCUS       DASTXO010000021         4035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_185266, whole genome shotgun sequence.
ACCESSION   DASTXO010000021 DASTXO010000000
VERSION     DASTXO010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185266"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1171)
                     /locus_tag="VFU96_00370"
     CDS             complement(128..1171)
                     /locus_tag="VFU96_00370"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU5111735.1"
                     /translation="MRAAVLKGWNDLSIEHVDVPDPGPGEVLLRVRACGLCGTDLKMV
                     HGAFEKRGWPPSLPFVMGHEWSGEVVLLGEGLDDLDLKPGDKVVAENHVGCGRCPMCR
                     QGRYNLCEKAGTPGYRLYGHTAPGALAEFAVRPAQMLHRLPDSVSPLEGALVNQGSLT
                     VHALRRVQFVPGSSVAIFGPGLLGLLAAGVASASGAAQVIMVGRGSRLDLAAKMGCDD
                     VVDYEKEDPVTGVRRLTSGRGVDYVFDCTGNADVPAQAIGAVRRGGRIALLGLTGGKP
                     AEIDVDRLTLDEIDLMGIRSSPNAYPAMIALMRSGKVDLSPLVTHVYPLESVGEALAA
                     LESREAVRPIVEL"
     gene            complement(1177..1977)
                     /locus_tag="VFU96_00375"
     CDS             complement(1177..1977)
                     /locus_tag="VFU96_00375"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEU5111736.1"
                     /translation="MIGDPDLNREHMLLANMTQPQAQEALARGTAVLVTGSIEQHGGH
                     LPLGTDAWAALTIALRVGERLEAPVVPISPVGVAPYHLHWPGSLSISPETLAALMVEV
                     CDGLARAGVERVIVVNWHEGNSTAVRLGADRAQQKLPLRVIVAESHVITNSLFPDEME
                     FTHAGAMETAAILAHDPTLVRLDESVEGDPIERATDGHALFRRRDVFPIMRDFREVAG
                     VGWYGTPESISEERAKEIIDAVAEHIVGAVEEVWKSLGERAPEDGKRT"
     gene            complement(2040..3089)
                     /locus_tag="VFU96_00380"
     CDS             complement(2040..3089)
                     /locus_tag="VFU96_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU5111737.1"
                     /translation="MSNSGRNGEPRSGKPPTLRDVAREAGVHVSTVSRVLNDQAAAGR
                     ITQDTDERIREVARRLGYRRNTIARALRTGRTLVVGMVVPDVANLYQAGITRGAGDVL
                     YAHGYSLILASTDDDPEHAESQVSAMLGVQADGLLYGVAREDDPVLSWLLDEGIPVVL
                     FNRGAQASGVSAVLPDDRTGTRMAVEHLLSLGHRHIVHVGGPEDVSSTVNRLGAFEET
                     LRTVGLRGHHSFAHRHIEEEGFRVTTDLLQDHPATTAVLAANDRLALGAIDAIRAAGR
                     SCPEDVSVVGFNDMPYGDRFSPPLTTVHISQNRLGAMAANLLLETIADPARPAETHLA
                     APELVVRGSTAPARG"
     gene            complement(3114..>4035)
                     /locus_tag="VFU96_00385"
     CDS             complement(3114..>4035)
                     /locus_tag="VFU96_00385"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5111738.1"
                     /translation="ISQVALAVYVASFLLGMCSATTNATGMTFFSNVTGASHRGTSMA
                     IFSAALLGGQAIGPAAAGLIASVGGWRFAMFVGTAAAGIVGLVLLTAGSSRPSPQNPV
                     RSGDVGLADQGSRALLPLLVLQSVSFAVFLTLGSVSQTMVPIIGADDLGLGTAAIGIA
                     LGVGGLARFVGTLVGGRLSDRLARKYVLVPGLLVQALGVALLSLNPTVTLWVTAILIM
                     SLASFAVPVAATILGDITDPARVGRQLGRFRFVGDLGLITGPLVVTSLFAGFGRAVAF
                     LFVAGILTTAALVAWRYLPETRGAHGQGSA"
BASE COUNT          740 a         1359 c         1281 g          655 t
ORIGIN      
        1 ccgcgctctg acacctttgc cacgcgatag gccgccacca cagcgagggc ctttgcctct
       61 tcctcggagc aggtgcacgc gctcgagccg aacatcgaga tgtgcggatg gtccaccaac
      121 gccatggtca gagctccacg atgggtcgga cggcctcacg agattcgaga gcggcgaggg
      181 cttcgccgac ggattccagg ggatagacgt gggtgaccag cgggctcaag tccaccttgc
      241 ccgaacgcat cagggcgatc attgccggat atgcgttggg actcgatcgg atgcccatga
      301 gatcgatctc gtcgagggtg aggcggtcga cgtcgatctc ggctggcttg ccgccggtca
      361 ggccgagtag ggcgatcctc ccaccacggc gaactgcccc aatcgcctgc gcgggcacgt
      421 ccgcgttgcc ggtgcagtcg aagacgtaat cgacaccacg cccggaggtg agacggcgca
      481 caccggttac gggatcttcc ttctcgtagt cgaccacgtc gtcacagccc atcttggccg
      541 cgaggtccag cctgctcccc cgcccgacca tgatgacctg cgctgccccg gacgccgacg
      601 ccaccccagc cgcgaggaga ccgaggagcc caggtccgaa tatggcgacg ctcgacccgg
      661 gtacgaactg gacccgtcgc agggcgtgca ccgtgaggga tccctgattg accagcgccc
      721 cctcgagagg gctgaccgag tcgggaagcc tgtgcagcat ctgagcgggt ctgaccgcga
      781 attcggccaa tgcacccgga gcggtgtggc cgtagaggcg atatcccgga gtgccggctt
      841 tctcgcatag gttgtacctg ccctggcggc acatggggca ccgaccacag ccgacgtggt
      901 tctcggcgac caccttgtcg ccaggtttca aatcgaggtc gtcgagaccc tcgccgagca
      961 agacgacctc tcccgaccac tcatggccca tcacgaaggg caatgacggg ggccagccgc
     1021 gtttctcgaa ggctccgtgg accatcttga ggtcggtgcc acagagacca cacgccctca
     1081 cccgaagcag aacttcacct ggtcccgggt ccggaacgtc cacgtgctcg atcgagagat
     1141 cgttccatcc cttcaagacc gcggcccgca tcgtggtcac gtgcgtttcc cgtcctcggg
     1201 agcccgctct cccagactct tccagacctc ctcgacggcg ccaacgatgt gctcggcgac
     1261 cgcgtcgatg atctctttcg cccgctcctc cgagatcgac tcgggtgttc cgtaccagcc
     1321 gacgcccgcc acttccctga agtccctcat gatggggaac acgtcacgac gtctgaatag
     1381 agcatgtccg tcggtggctc gttcgatcgg gtcgccctcg acgctctcat cgagcctcac
     1441 caacgtggga tcatgggcca ggatcgcggc cgtctccatc gccccggcat gagtgaactc
     1501 catctcgtcg gggaacagcg agttggtgat gacatgggac tcggcgacga tcacccgcaa
     1561 tgggagtttc tgttgggctc ggtcagcacc gagccggact gccgtcgagt tgccctcgtg
     1621 ccaattcacg acgatcaccc tctccactcc ggcccgagcg agaccgtcgc agacctccac
     1681 catgagggca gccagtgttt caggactgat gctcaacgat cctggccagt gaaggtgata
     1741 cggagcaacg cccactggcg agatgggcac caccggcgcc tcgagacgct cgcccactcg
     1801 cagggcgatg gtgagagccg cccaggcgtc ggttcccaag ggaaggtgac ccccatgctg
     1861 ttcgatgctc cccgtcacca gcaccgccgt tccccgagcc agcgcttctt gcgcctgagg
     1921 ctgggtcatg ttggccagaa gcatgtgctc gcggttcaag tccgggtctc cgatcatcac
     1981 cgtcaggtca ccctgcgcaa acggttgttc agggagacta gccctcgcta tttcgactgt
     2041 cagccccggg ccggagccgt cgagcccctg accacgagct cgggtgcggc gagatgggtc
     2101 tccgccggcc gcgccgggtc cgcgatcgtc tccaacagga ggttcgccgc catcgccccc
     2161 agccggttct gggagatgtg gacggtcgtc agaggtggtg agaagcggtc gccgtatggc
     2221 atgtcgttga agccgacgac cgagacgtcc tccgggcacg atcgtccagc agcccggatg
     2281 gcatcgatcg cccccagcgc gaggcgatcg ttggctgcga ggacggcggt cgtagcggga
     2341 tggtcctgga gcagatcagt cgtcactctg aatccctcct cttcgatatg acggtgggca
     2401 aaggagtgat gtcctctcag accgaccgtc cggagggttt cctcgaaggc cccgagccga
     2461 ttgacggtgg acgagacgtc ctccggcccg ccgacgtgca cgatgtggcg atgacccaag
     2521 gagagcaggt gctccaccgc catccgggtc ccggtgcgat cgtcggggag aaccgcgctc
     2581 accccgctgg cttgggctcc gcgattgaag agaaccaccg ggatcccttc atcgaggagc
     2641 caggagagaa ccggatcgtc ttctctggcc acgccataga gcaggccgtc cgcctggact
     2701 ccgagcatgg ccgagacctg ggactcggcg tgctccgggt cgtcgtccgt gctggcgagt
     2761 atcagggaat acccatgggc gtacaggaca tcccctgcgc cccgggtgat accggcctgg
     2821 tagaggttgg cgacatccgg cacgaccatc cccacaacca gcgtgcgccc ggtgcgaagg
     2881 gcgcgggcga tggtgttgcg acggtatccg agccttcggg ccacttcccg gattcgctcg
     2941 tctgtgtcct gggtgattcg acccgcggcg gcctggtcgt tgaggacacg ggataccgtc
     3001 gacacgtgca ccccagcttc gcgggcgacg tcacgcagcg tgggtggctt gccggagcgc
     3061 ggctcgccat ttcgacccga gttgctcacg gggcgagagt aacccgagcg atctcacgcc
     3121 gatccctgtc catgtgcacc ccgcgtctcg gggagatacc tccatgcaac cagggcggcc
     3181 gtcgtcagga ttccggccac aaacagaaac gccactgctc taccgaagcc ggcgaacagg
     3241 ctcgtcacca cgagcggccc cgtgatcaat ccgagatccc cgacgaagcg aaagcgaccc
     3301 aattgccttc cgacccgggc agggtcggtg atgtccccga gaatcgttgc cgccacgggg
     3361 acggcgaagc tggccagcga catgatgagg atggcagtga cccacagagt cacagtgggg
     3421 ttgagcgaga gcagtgccac accgagggct tggaccaaca gtcccgggac gagcacatac
     3481 ttccttgcga ggcggtcgga cagccgcccg ccgacgaggg ttcccacgaa tcgcgccagc
     3541 ccgccgacgc cgagtgcgat tccgatggca gcggtgccca gtccgaggtc atcggctccg
     3601 atgatcggca ccatcgtctg ggaaaccgag cccagggtca ggaagacagc gaacgacacg
     3661 ctttgcagga cgagcaatgg cagcaaagcc cggctcccct gatcagcgag ccccacatct
     3721 cccgatcgca ccggattctg tggagagggc cggctcgaac ccgctgtcaa gaggacgaga
     3781 cccacgattc cagccgcggc ggtgcctacg aacatcgcaa accgccaccc accgaccgat
     3841 gcaatcagac cggccgctgc cggcccgatg gcctgcccgc cgagcaacgc ggccgagaag
     3901 atcgccatcg aagtgccgcg atgactggcg ccggtcacat tggagaagaa cgtcatcccg
     3961 gtggcattgg tggtcgcgga gcacatcccc agcaggaagg aagcgacgta gacggccaac
     4021 gcgacttggg agatc
//
LOCUS       DASTXO010000022         4187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_37916, whole genome shotgun sequence.
ACCESSION   DASTXO010000022 DASTXO010000000
VERSION     DASTXO010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37916"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VFU96_00390"
     CDS             complement(<1..502)
                     /locus_tag="VFU96_00390"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEU5111739.1"
                     /translation="MLPPFWVIGAPRSGTTFLARVLDHHPEIFLTNETRVMLLFSRML
                     NRWVEARRLLATSHDQIVDSLWRQLPGVVEQIYRDLGVAPGQRWGDKYPQYADGDLDP
                     EALSTIDRLFPESQYVHIIRDPRAVVASILAKGWLEFDEAIEAWRVFVLHGREFGTSL
                     GDGRYHE"
     gene            complement(502..1251)
                     /locus_tag="VFU96_00395"
     CDS             complement(502..1251)
                     /locus_tag="VFU96_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111740.1"
                     /translation="MPLDQTTVIMFHVGRCGSTVVAEMLGRHPRVSWDGEVFEPAVRR
                     KIGLEELGPDEFLRQRRHATSEAYGFEIKYLPSHHQRLLGLDLDGLLDLSRRVGVTHY
                     VTLHRHNYLRRVVSGAIGRERRRWHRSQSEPVPSTEVRLDPMAVPFGPRQPLLAAFEE
                     MRVGEDALHQALEGRPVLHLVYEDHVESDPAVAYRLICDLIGIEAGNIGAQLGRTGDA
                     PLTELLVNFEEIADVLTGTEYEWMLDKQTTS"
     gene            1408..2265
                     /locus_tag="VFU96_00400"
     CDS             1408..2265
                     /locus_tag="VFU96_00400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5111741.1"
                     /translation="MTAPDLSVVVLTWNQLELTRRCVRSLRAGTSSAYELIMVDNGST
                     DGTADFIASEADISVLNQSNLGFAPGMNAGLKEARGDVTVFANNDTEFPSGWDLPLVE
                     DFSTHPRAGVVLPAVTAAGNPVSVRSTPGQTVESLLPFGEFPSGVVYLVRTELMRQLG
                     GWNEAFEGASAEDLDLVFTMWAHGLDVILDTRVLIEHVSQASMRERPDLSALYQRNLG
                     LFLDRWESGPDGPLLDTVTSDDYERNLERARTAVVWIRRMIAARQEAAQSRTEPDTPT
                     KKGRFRSRS"
     gene            complement(2249..3088)
                     /gene="rfbD"
                     /locus_tag="VFU96_00405"
     CDS             complement(2249..3088)
                     /gene="rfbD"
                     /locus_tag="VFU96_00405"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEU5111742.1"
                     /translation="MSAVAIIGAGGQLGTAYTRLLGEKALPLGRGDLDLRNVERIANV
                     LDHLDPSTVINCAAYTAVDRAESEEDLATLINGEAVGQIADWAQRRGRPFLTFSTDYV
                     FDGAADRPYVESSPTNPINAYGRSKLVGEELALPRGALVVRTSWVISGSNPNFVATIL
                     RKVREDEVRVVDDQRGNPTVAVDLAGASYAALRSGASGLIHLTNQGETTWYELARTSV
                     GLAGLDPSRVSPCETVDYPTPAPRPGYSVLASERLEGLGLEPLPHWRESIGGVVDEIK
                     TWI"
     gene            complement(3076..3951)
                     /gene="rfbA"
                     /locus_tag="VFU96_00410"
     CDS             complement(3076..3951)
                     /gene="rfbA"
                     /locus_tag="VFU96_00410"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEU5111743.1"
                     /translation="MRGIVLAGGAGTRLDPMTRVASKQLQPVYDKPMIYYPLATLMEA
                     GVSEILIISTPKDTPRFEELLGDGSPWGLSIEYAVQERPGGIAQAFLVGASFVGGEPV
                     ALILGDNIFHGALGLAEIVEAFVGGALVFGYPVSDPRRYGVVELAADGKVLSLEEKPD
                     RPRSNLAVPGFYLYDERVVEMTKALRPSARGELEITDLNRAYMEEGSLRAVRLGRGIA
                     WLDSGTHDSLLEAANFIATIEHRQGLKIACLEEIALDRGLVTVAEMDETLDAMPLSTY
                     RDYVADVLEEHRSAQ"
     gene            complement(3948..>4187)
                     /locus_tag="VFU96_00415"
     CDS             complement(3948..>4187)
                     /locus_tag="VFU96_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004931395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEU5111744.1"
                     /translation="QMSNLELTRRLLEIMGADDTAIEYVEDRKGHDLRYAVDSSKLRA
                     LGWSPADSFEERLADTVAWYRSREDWWRPLKQASS"
BASE COUNT          783 a         1366 c         1267 g          771 t
ORIGIN      
        1 gttcgtggta tctcccatcc cccagcgaag ttccgaactc ccgcccgtgc aagacgaaga
       61 cgcgccaggc ctcgatagcc tcgtcgaact cgagccagcc tttggccagg atcgatgcca
      121 ccactgcccg gggatcgcga atgatgtgaa cgtactggct ctccggaaac agacgatcga
      181 tggtggagag cgcctcagga tctaggtccc cgtccgcata ttgcggatac ttgtcacccc
      241 aacgctggcc aggggcgacg cccaggtccc gatatatctg ctccacgacc ccgggcaact
      301 gcctccacaa gctgtcgacg atctgatcgt gtgacgtggc caggagacgt cgcgcttcga
      361 cccatctgtt cagcatccgg ctgaacaaca gcatcaccct cgtctcattg gtgaggaaga
      421 tctccggatg atgatcgagc accctggcca ggaaggtcgt gcccgatctt ggagcgccga
      481 tcacccaaaa ggggggcaac atcagcttgt ggtctgcttg tccagcatcc actcgtactc
      541 ggtcccggtg agaacgtcgg ctatctcctc gaagttgacc aagagctccg tcagaggtgc
      601 atcgccggta cgtcccagct gcgccccgat attgcccgcc tcgatgccga tcagatcgca
      661 gatcagccgg tatgccacgg ctggatcgct ctccacgtga tcctcgtaga cgaggtgcaa
      721 cacgggacga ccctccagtg cctgatgcaa tgcgtcctcg ccgactctca tctcctcgaa
      781 agccgccagc aagggttggc ggggcccaaa agggactgcc atgggatcga gccgcacctc
      841 ggtgctcgga accggctcgc tctgggatcg atgccatctc cgccgctccc ggcctatcgc
      901 cccagagacc acccgacgca agtagttgtg gcgatgaagg gtcacataat gggtcacgcc
      961 gactcggcgt gacagatcga ggaggccatc gagatccaga cccaggagac gctggtggtg
     1021 tgacggcaag tacttgatct cgaagccata ggcctcgctg gttgcatgtc tgcgctgccg
     1081 gagaaactcg tccgggccga gctcctcgag cccgatcttc cggcgcaccg ccggctcgaa
     1141 gacctccccg tcccacgaca cccgggggtg gcgccccaac atctcggcca cgacggtgct
     1201 cccgcaacgg cccacatgga acatgatgac ggtggtctgg tcgagcggca cgttggggac
     1261 tttaagactg tcgaattggt ccccagatgt tccctacgag gaagcgcccg attcgttgct
     1321 ccctatcgcc tgattttcga gcgacctggc ggcgacgtgg tcatccaccg ctccatggcg
     1381 gtgcatgtgc ctagcctccg gtgacgcgtg acggcacccg atctctcagt ggtcgtgctg
     1441 acctggaatc agttggagct gaccaggagg tgcgtgcgat ccctgcgggc gggcacgtcg
     1501 tcggcgtacg aattgatcat ggtcgacaac gggtcgaccg acggcaccgc tgacttcatc
     1561 gcatctgagg cggacatctc tgtcctcaac caatcgaacc tgggattcgc cccggggatg
     1621 aacgccggtc tcaaagaggc cagaggcgac gtcacggtct tcgccaacaa cgacaccgag
     1681 ttcccgagcg gctgggacct tccgctggtc gaagatttct ccacgcatcc tcgagccggg
     1741 gtggtgctac cggcggtcac ggccgcgggc aatcccgttt ccgtcaggtc cacgccgggc
     1801 cagacggttg agagcctcct cccctttggt gagttcccca gcggggtggt ctatctggtc
     1861 cgcaccgagt tgatgagaca actgggcggc tggaacgagg cgttcgaagg tgccagcgcc
     1921 gaggacctag atctcgtatt cacgatgtgg gcccatggcc tcgacgtcat cctcgacacc
     1981 agagttctga tcgaacacgt gtcccaggcc agcatgcgtg agcggcccga cctctctgcc
     2041 ctgtatcagc gcaatctggg gctctttctc gatcgttggg aatctggtcc agatgggccc
     2101 ctcctcgaca ccgtcacgtc cgatgactac gagcgaaacc tcgaacgcgc caggaccgca
     2161 gtcgtatgga tacgccgcat gattgccgcg aggcaagaag cggcccaatc gcgcaccgag
     2221 ccggacacgc cgacgaagaa aggccgcttc agatccaggt cttgatttcg tccacgacac
     2281 cgccgatcga ctctcgccaa tggggaagag gttcgagccc gagtccttcg agccgctccg
     2341 acgcgaggac cgagtacccg ggccgcggag cgggcgtcgg gtagtccacg gtttcgcatg
     2401 gtgagaccct ggacgggtcg aggccggcga gccccacact cgtcctggcc agttcgtacc
     2461 acgtggtctc cccctgattg gtgagatgaa tcagtccgga cgcccccgac cggagagcgg
     2521 cgtagctggc cccggccaga tcgaccgcca cggtcgggtt gccccgttga tcgtccacca
     2581 cccgcacctc gtcctctcgg accttccgga ggatcgtggc gacgaagttg ggattgctgc
     2641 ccgagatcac ccatgaagtc cgcacgacaa gcgccccccg gggcagagca agctcctccc
     2701 ccaccagctt ggagcgtccg taggcgttga tcgggttggt cggggatgac tcgacatacg
     2761 gccggtcggc ggccccatcg aagacatagt cggtggagaa cgtgagaaac ggccgacccc
     2821 gtcgctgagc ccagtcggcg atttgcccta ccgcctcacc gttgatcagc gtggcgaggt
     2881 cttcctccga ctctgcccgg tcgacggctg tataggccgc acagttgatg acagttgaag
     2941 gatcgagatg gtccaagaca ttcgcgatcc tctcaacgtt tctgagatcc aggtcaccac
     3001 ggcccagggg aagcgccttt tcaccgagga gccgggtgta ggcggtgccc aactgacctc
     3061 ccgcaccgat gatcgctact gcgctgatct gtgctcctcg agcacgtcgg ccacgtagtc
     3121 gcggtaggtg gagagaggca tggcgtcgag ggtctcgtcc atctctgcca ccgtcaccag
     3181 gcctcggtcg agggcaatct cctctagaca agcgatcttg agaccctgcc tgtgctcgat
     3241 ggttgcgatg aagttcgccg cttccagcaa cgagtcgtgg gtgccactgt cgagccaggc
     3301 gatcccccgc ccgagacgca cggcgcgaag acttccctct tccatgtacg cccggttcag
     3361 atccgtgatc tccagctcgc ctctcgccga tgggcgcaag gccttggtca tctcgaccac
     3421 ccgctcgtcg tacaaataga agccgggcac ggcgagattg gatcgaggtc tgtcgggctt
     3481 ctcctcgagg gacagcactt tcccgtcggc agcgagctcg acaacaccgt atctgcgagg
     3541 gtcggagacc gggtacccga agacgagggc gccccccacg aacgcctcga cgatttcggc
     3601 aagccccaga gccccgtgaa agatgttgtc cccgaggatc agtgcaacgg gctcaccacc
     3661 aacgaagctc gcccccacga gaaaggcctg ggcaattccg ccgggccgtt cttgcaccgc
     3721 atattcgatc gagagccccc agggcgagcc atcgccaagc aactcctcga atctgggtgt
     3781 gtcctttggt gtggagatga tgagtatctc cgagacgccg gcctccatga gggttgccag
     3841 ggggtaatag atcatcggtt tgtcgtagac cggttgtaac tgcttgctgg ccacgcgtgt
     3901 catcgggtcg agccgggtcc cggccccacc ggcgaggacg atgcccctca agaggacgcc
     3961 tgcttcaacg gtcgccacca gtcttcgcgg ctgcggtacc acgcgacggt gtcggcgaga
     4021 cgctcttcga atgaatcagc aggagaccac ccgagagctc gtagcttcga ggaatcgacg
     4081 gcgtacctga gatcgtgacc tttcctatct tcgacgtatt cgatggcggt gtcgtctgct
     4141 cccatgatct cgaggagacg acgcgtcaac tcgagattcg acatctg
//
LOCUS       DASTXO010000023         3017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_295855, whole genome shotgun sequence.
ACCESSION   DASTXO010000023 DASTXO010000000
VERSION     DASTXO010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295855"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="VFU96_00420"
     CDS             <1..977
                     /locus_tag="VFU96_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU5111745.1"
                     /translation="RSAGRTQGVCVGEEWRVRLTAIVLAAAGTTALAVGLLVYSLFPP
                     ARSLASRIGPYLNPTRATSTRPNPAGPLGAVFGPMFRSLAASMGRMLERSGENVTIVE
                     LRQAGWYRDLPVEETVQAFRMAQLRTIASVAGLTFVLGIVLSLSPIQRIVLVTLGVVI
                     GATRQKASLHSAIDRRRERMCIEIYTVNQLLAMRVRAGGGVVHAVQDVVRRGEGEVVA
                     ELSDALRLHRSGWRAADAFRRIAELTPEPFCGRTYRLLATAEERGADLAPALLGLAED
                     VRETRRETMRRSATKRRAAMLVPTIAILAPVLLLFVAAPLPYLITGWR"
     gene            990..1169
                     /locus_tag="VFU96_00425"
     CDS             990..1169
                     /locus_tag="VFU96_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111746.1"
                     /translation="MLALKQWLREAWGDDRGLNTAELMGNAALAIAALVVIWGALQAL
                     GVDIVGWIRSQLIGG"
     gene            1139..1531
                     /locus_tag="VFU96_00430"
     CDS             1139..1531
                     /locus_tag="VFU96_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111747.1"
                     /translation="MDPLSADRRLIDRGITSVQFLLASGLGMLLFLALANLVVVQYGR
                     GALRSALDQGARAGAINSSEDECRARIEDVLGQLLGGRMGDSVVTSCGVGPAVVSATG
                     RAVFVSWTPFTPDFVIEMTALATRESPP"
     gene            1528..1956
                     /locus_tag="VFU96_00435"
     CDS             1528..1956
                     /locus_tag="VFU96_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111748.1"
                     /translation="MSDKGHAAVELGLAVGILLLPAALAVLAFGPWSERRVFAEAAAA
                     EAARVAVIELDSSAGTQVVMEMTANQGLSNDLVRLGWCGATGSDSSPQPDDCTFDRGS
                     VVTAEVQVWVPLIATPWGEVGGLWVGASHSEPVDLYRSIS"
     gene            1953..2369
                     /locus_tag="VFU96_00440"
     CDS             1953..2369
                     /locus_tag="VFU96_00440"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEU5111749.1"
                     /translation="MKSERGSVTLWTLGLSMLLLLFGGVAIDFWRALALQRELAAIAD
                     SASVAAASGIDEDHYRMTGEVRIDAARATEIGSSYVVSQDVALEDLVVTTASDGLSVS
                     VLVVDHLDLGLIGVFVDQRAPLTVTAEATAIPVLVP"
     gene            2442..2516
                     /locus_tag="VFU96_00445"
     tRNA            2442..2516
                     /locus_tag="VFU96_00445"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2475..2477,aa:Gln,seq:ctg)
     gene            2541..2613
                     /locus_tag="VFU96_00450"
     tRNA            2541..2613
                     /locus_tag="VFU96_00450"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2575..2577,aa:Glu,seq:ctc)
     gene            complement(2641..>3017)
                     /locus_tag="VFU96_00455"
     CDS             complement(2641..>3017)
                     /locus_tag="VFU96_00455"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEU5111750.1"
                     /translation="IRKVAGDGHVNHGYWVVPVPRAFRWLVGGAKRIGEHRLVMAQHL
                     GRALRPDEVVHHRNGKRTDNRIENLELWSTAHPKGQRVEDLVAFGVEILARYAPEIGT
                     WVLGRTGASPMDTTSGERPSRI"
BASE COUNT          463 a          916 c         1020 g          618 t
ORIGIN      
        1 ggcgctcagc cggcagaacc cagggtgttt gtgtcggcga ggagtggaga gtgaggctga
       61 cagcgatcgt gctggccgca gctgggacca cggcactggc ggtcggtctg ttggtgtact
      121 cgctgttccc tccggctcgc agtctcgcct cgaggatcgg tccctatctg aacccaaccc
      181 gagcgacgtc aacacggccc aacccggccg gcccccttgg cgccgtcttc gggccgatgt
      241 tccgctcgtt ggcggcatca atgggtcgga tgctcgagag gtcgggggag aacgtgacga
      301 tcgtcgagct gcgtcaagct ggttggtacc gcgaccttcc cgtggaggag acggtccagg
      361 cgttccggat ggcgcagctt cggaccatcg cgtctgtcgc cggcctgacg tttgtcctcg
      421 ggatcgtcct cagcctctcg ccgatccagc gcatcgtgct cgtaaccctc ggcgttgtga
      481 tcggggcgac tcgccagaag gcatcgcttc actcggccat cgatcgaaga cgagagagaa
      541 tgtgtatcga gatctacacc gtgaaccagc ttctcgccat gcgggtgcgg gccggaggcg
      601 gtgtggtgca cgcggttcag gacgtcgtgc gccgcggcga gggggaagta gtcgccgagc
      661 tctccgacgc gctgcgcttg caccggtcgg gatggcgtgc cgccgatgct tttcggagaa
      721 tcgccgagtt gacgccggag ccgttttgtg gtcgcaccta tcgacttctg gccaccgcgg
      781 aagagcgagg ggccgacctt gcaccggccc tgctcggcct ggccgaggat gtcagggaga
      841 cacgtcgtga gactatgcgg aggtcggcga ccaaacgccg ggcggcgatg ctggtgccaa
      901 cgatcgccat cctggccccg gtcctcctgc tcttcgtggc tgctcctctt ccgtacttga
      961 tcacaggctg gcgatgaatg gaggttgaca tgctggccct caagcaatgg ctccgcgagg
     1021 cgtggggcga cgaccgggga ctgaacaccg ccgagctgat ggggaatgcc gccctggcaa
     1081 tagccgcact ggttgtgatc tggggcgccc ttcaggcgct cggcgtggac atcgtcgggt
     1141 ggatccgctc tcagctgatc ggaggctgat cgatcggggg atcacctcgg tccagttcct
     1201 cctcgcgagc ggactgggaa tgctgctgtt cctcgccctg gccaatctcg tggtcgtcca
     1261 gtacggccgc ggtgcccttc gtagcgcact cgaccaggga gcccgagccg gtgcgatcaa
     1321 ctcgtcggag gatgaatgcc gggcgagaat cgaggatgtc ttgggccagt tgctgggagg
     1381 ccgaatgggc gactcggtcg tgacgtcgtg tggggtaggg cccgccgtcg tgagcgcgac
     1441 cggccgcgcg gtcttcgtct catggactcc gttcactccg gacttcgtca tcgagatgac
     1501 ggccctggcg acgagggagt caccgccttg agcgacaagg ggcacgcggc tgtggagttg
     1561 ggtcttgcgg tggggatcct cctcctgccg gcggcacttg ccgtcctggc cttcgggccc
     1621 tggtctgagc gccgagtatt tgccgaggcc gcggctgcgg aggcggcaag agttgcggtg
     1681 atcgagctcg acagttcggc tggcactcag gttgtgatgg aaatgacggc gaatcaagga
     1741 ctgagcaacg acctcgttcg tctcggttgg tgcggcgcta ctgggtccga ctcgagccct
     1801 cagcctgatg attgcacctt cgatcgtggc tcggttgtga ccgccgaggt gcaggtctgg
     1861 gttcccctga tcgccacgcc gtggggtgag gtcggtggtc tttgggtcgg agcctctcat
     1921 tccgagcccg ttgatctgta taggagtatt tcttgaagtc cgagcgaggt tcggtcacgt
     1981 tatggacgtt gggcttgtcc atgttgttgc tcttgttcgg aggcgtggcg atcgacttct
     2041 ggcgcgcttt ggccctccag cgcgaactcg ccgcgatcgc cgattcggcc tcggtggcgg
     2101 cggcgtcagg tatcgacgaa gaccactatc gaatgacggg cgaggtgcgg atcgatgcag
     2161 cacgggccac cgagatcggt tcgtcatatg ttgtctcaca agacgtggct ctcgaggacc
     2221 tggtcgtcac caccgcctcg gatggtctgt ccgtatcggt gttggtagtc gatcacctcg
     2281 atttggggct cattggcgtg ttcgttgatc agcgtgcgcc cctgacggtc accgcagagg
     2341 cgactgcgat ccccgtcctg gtcccttgag ggagagttcg cactcgccct gccgccgatt
     2401 atccttgacg cctaccctgc atcgccttcg ggtgtgcgcg ttcgggggtg gtgtaatagg
     2461 taacacgaca ggttctggcc ctgttattga gggttcgagt ccgtcccccc gagccaggcc
     2521 ggcgagagcc ggcccaacct ggtcccgtcg tctaggggtc gaggacgccg gcctctcaag
     2581 ccggaaacgc cggttcaaat ccggtcggga ctgcaaaacc cctggtagag ggctattttt
     2641 ctagattctg gatggtcgct cgcccgaggt ggtatccatt ggagaggcac cagtccgacc
     2701 caagacccac gtaccaatct ccggggcgta tcgggcaagg atctcaacgc caaaagcgac
     2761 caggtcctct acccgctgtc cctttggatg agcggtcgac cacagctcca ggttctcgat
     2821 ccgattgtcc gtgcgcttgc cattccggtg gtggaccacc tcgtcgggcc ggagggcacg
     2881 tccgagatgt tgggccatga ccagtcgatg ctctccgatc ctcttggccc ccccaacgag
     2941 ccaacggaat gcccgtggca ccgggacgac ccagtagcca tgattcacat gtccgtcacc
     3001 ggccacctta cggatcg
//
LOCUS       DASTXO010000024         2164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1695963, whole genome shotgun sequence.
ACCESSION   DASTXO010000024 DASTXO010000000
VERSION     DASTXO010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1695963"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="VFU96_00460"
     CDS             <1..1125
                     /locus_tag="VFU96_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5111751.1"
                     /translation="VAGRAGRAPGALVEGALGLHASFGLRRGESFRLDLTLSIPPGST
                     VALLGPNGAGKSTAVAAIAGLLAIESGAIRLAGNPLDEPANDLFIPPEDRRIGVVFQD
                     YLLFPRLTVIENVAFGLRSRGVSRTEAMQRAKNWLDRLELTGLDRRKPPQLSGGQAQR
                     VALARALVIEPDLLLLDEPLSALDVTTRVELRRVLARHLEEFPGPRLVITHDPTEAFL
                     LADQVHIIENGAVTQAGSPDEIRLRPRTPYAADLAGSNLLIGHASDGKVEVGSHLIHL
                     AEQVIEGEVLLTIRPSAISLHRDPPEGSPRNTWQTSIVVMERLGERTRVRTGGPIPLT
                     VEVTSEAAMALELAEGERVWLAIKATEIGVETGGVSTRSG"
     gene            1166..1795
                     /locus_tag="VFU96_00465"
     CDS             1166..1795
                     /locus_tag="VFU96_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU5111752.1"
                     /translation="MGEKQRISYVPLENMTPEMREEMERCAQEGTPRPESSAIRAHVP
                     AAFWFFAESWQNLFRDGILDHEIKELCRVYVSRSVKCEYCGNQRSEKGKALGLVEGQY
                     DDLMNFERSERFDDRQKAALAYTEAIAWHLETDDEFWERMNQYFNEPELVELGCFVGL
                     TLGQQSWLRLLNIDHHEVMPGTEAAMAPGFEDAESLARSKSTEGYWAKV"
     gene            complement(1829..>2164)
                     /locus_tag="VFU96_00470"
     CDS             complement(1829..>2164)
                     /locus_tag="VFU96_00470"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEU5111753.1"
                     /translation="GALGIAVWIIDGLEFTGSWWQYVIVVVILGVANFIVKPILRLLS
                     LPIILITLGLFLIVVNALVLQLVVWLSGIWDLGLTSTGFFWATFLGAIVLSIATWLLG
                     IIVPDGDRT"
BASE COUNT          425 a          637 c          723 g          379 t
ORIGIN      
        1 gtcgctggcc gtgctggtcg ggctccggga gcgttggtgg aaggcgctct agggctccat
       61 gccagtttcg gtctgcgccg tggcgagtca ttccgcctcg acctgactct ttcgataccc
      121 cctgggtcca cggtcgccct acttgggccc aatggagcag ggaagtcgac ggcggtggcg
      181 gccatcgccg gactgttggc gatcgaatcc ggtgcgatca ggctcgccgg gaaccccctg
      241 gacgagccgg cgaatgatct gttcatcccg ccggaggacc gcaggatcgg ggtcgtcttc
      301 caggattatc tgctgttccc ccgcctgacc gtgatcgaaa acgtcgcctt cggcttgcga
      361 agccgggggg tgagccgcac cgaggctatg cagagagcca agaactggct ggaccggctc
      421 gagctcaccg ggctcgatcg caggaaaccg cctcagctgt ccggtggtca ggcgcagcgg
      481 gtcgcgttgg cccgggcgct ggtgatcgag ccggatctcc tcctgctcga cgagccattg
      541 tcggctctgg acgtgacgac ccgggtcgag cttcgacggg tcctggcccg tcatctggag
      601 gagttccccg gtccacgtct cgtcatcact cacgacccga ctgaggcgtt cctcctcgcc
      661 gaccaggtcc atatcatcga aaacggcgca gtgacgcagg ccggatcccc ggacgagatc
      721 cgtcttcggc cacgaacgcc ctatgccgcg gacctcgccg ggtcgaatct tctgataggc
      781 catgcctccg atggcaaggt agaggtgggg tcgcatctga ttcacctggc ggaacaggtg
      841 atcgaaggtg aggtgttact gacgatccgg ccatcggcga tctcgttgca ccgcgacccg
      901 cctgagggca gcccccgcaa cacgtggcag accagcattg tcgtgatgga acggctgggg
      961 gagcgcaccc gggtccgcac cggcgggccg attccactca cggtcgaagt gacctcggag
     1021 gcggcgatgg ctctggaact ggctgagggc gaaagggtgt ggctggcgat caaggccacc
     1081 gagatcggcg tcgagaccgg gggagtgtct acccgttccg ggtaacctct ggttgcttcg
     1141 acgcacacga ctggagacga ccgccatggg tgagaagcaa cgcatcagct acgtgccgct
     1201 cgagaacatg acgccggaga tgcgagagga gatggagcgt tgcgcccagg aggggacacc
     1261 gaggccggag agctcggcga ttcgggccca tgtcccggcg gcattctggt tcttcgcaga
     1321 aagctggcaa aacctgttcc gcgacgggat cctcgatcac gagatcaagg aattgtgccg
     1381 ggtctacgtg tcccggtccg taaaatgcga gtactgcgga aatcaacgct cggagaaggg
     1441 aaaggcgttg ggcctggtcg agggccaata cgacgacctg atgaacttcg agcgctccga
     1501 gcgctttgac gacagacaga aggcggcatt ggcctacacc gaggcgattg cctggcatct
     1561 cgaaaccgat gatgagttct gggagcggat gaatcaatac ttcaacgagc ccgaactggt
     1621 ggagcttggc tgtttcgtcg ggttgacctt gggtcagcag agctggctgc gattactcaa
     1681 catcgatcat catgaggtga tgcccgggac cgaagccgcc atggcacccg ggttcgagga
     1741 cgcggagtct ctggccagga gcaagtcgac agagggctac tgggccaagg tctgatctgg
     1801 acggaggctt ggaaccgact cgaatccttc aggttcgatc gccatcaggc acgatgatcc
     1861 caaggagcca ggttgcgatc gagagaacga tggcgccgag gaacgtcgcc cagaagaacc
     1921 ctgtcgaggt gagaccgagg tcccaaatcc cggatagcca caccaccagt tggagcacga
     1981 gggcgttgac gacgatcagg aacagtccca gggtgatgag gatgatcggg agactcagca
     2041 gacgcaggat tggcttcacg atgaagttgg ccaccccgag gatgaccacg acgatgacgt
     2101 attgccacca gctcccggtg aattccagac cgtcgattat ccagaccgcg attccgaggg
     2161 ctcc
//
LOCUS       DASTXO010000025         4355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_332741, whole genome shotgun sequence.
ACCESSION   DASTXO010000025 DASTXO010000000
VERSION     DASTXO010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332741"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..352
                     /locus_tag="VFU96_00475"
     CDS             92..352
                     /locus_tag="VFU96_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111754.1"
                     /translation="MFEAVEFTEVRGRDEPDQRVIGTYPDEISAVEAVRSAKTEFVES
                     GSEDFAWWVVRQPGATLAQFISDSRSDKEFVLDLTTGQLIEV"
     gene            422..1972
                     /locus_tag="VFU96_00480"
     CDS             422..1972
                     /locus_tag="VFU96_00480"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEU5111755.1"
                     /translation="MRTAEVIAAACLATDLGMGFPFEHGLQATLVTCRLAERLAVGPK
                     TAAEAFYVSLLMYSGCTTDSDIATGIFGGSLTRNMTARQFGSTLEAMGGVIRALPDPD
                     SGIFKSGLEIAANLPKAAMFRSTHFTALCEVAMMLSERLGLPDEINRLFLYLTERWDG
                     KGVLGRKAGDDIPIALRIVTVARDAAYQTLMGGEEHAARVIKDRAGHAFDPDVAEALS
                     HNASEMLSTDEDGPSVWDSVLDAEPGPHLTLDSGAVDRALGALGDFADLVSPSLVGHS
                     TGVARLAGEAASDLGMTPEEVTNIRRAGLIHDIGRVAIQPRTWQKNSSLNADEWEQVR
                     LHAYHTERVFNRSPFLAPVAAVACRHHERMDGSGYHRGLNATSLEPASRLLAAADAFH
                     AMTEPRDHRPPLPLADAGEVLRVESAAGRHDGEMVAAVLRAAGLQTSPVERPAGLTER
                     EVQVIRLLARGLQTKQVARTLDISAKTADRHIQNAYRKIGVSTRAAATLYASEHGLVQ
                     WGELPISL"
     gene            2347..3447
                     /locus_tag="VFU96_00485"
     CDS             2347..3447
                     /locus_tag="VFU96_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018760604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEU5111756.1"
                     /translation="MPTSQERIPTVVIGGGQAGLSVGYHLRRLAVPFVILDAQERVGG
                     AWRGRWDSLRLFTPARFDGLDGKPFPAPSHAFPTKDEMADYLEAYSDEFHLPVRSGQR
                     VKSLTKRDSVFIVETDDVRFEAQSVVVAMSSWQVPRIPGFAKELRPDIVQIHAADYRN
                     LSQLQEGPVLVVGAGNSGSEIAIDVVGSHETVVAGPDTGHVPFRVEGRVGRLLVPVVR
                     FVFHRVLTKSNPIARRRMPKMIASGEPLVRTKPRDLMEAGIERAPRVVGAEDGLPVLE
                     DGRVIHAANVIWCTGFEPGFTWIDLPVFEDGRVKHDRGVADVFGLYFVGLKGLFSVSS
                     AQVNGVGRDAERVAKLIAATMSPAPAPAGARR"
     gene            3444..>4355
                     /locus_tag="VFU96_00490"
     CDS             3444..>4355
                     /locus_tag="VFU96_00490"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEU5111757.1"
                     /translation="MRADRVLVVGGGLAGLSAGIALARAGSHVEIVEKADQWADVGAG
                     MYLPGNAHRALESLGIGTVMRDRAFQIKHQIFSDHAGRTLADVDLHQVWVGVGPCLAI
                     ARADLHRALRDAARGVDMRMGVEVESLEQDGGSAVVDFTDGSSGEFDLLVGADGLNSS
                     VRRHMSGKSEPSPVGQVSWRFITTGDAGLSNWTVMLGGEKTFLAVPLGGGRVYCYLDV
                     SSHDGADPTEGDPGRLEEMFGDFAAPATAFIRAATESGSVHFGVIEEVVANDWVTGRV
                     VLVGDAAHATSPNMAQGAAMAFEDAVVL"
BASE COUNT          737 a         1360 c         1476 g          782 t
ORIGIN      
        1 acatggaagt cgctcaagaa aaaatgagca tagggaccca gggcaaatgg cccgtcgtcg
       61 gctatagaat ctgtggtcag gaggatcgtg aatgttcgag gcggtggaat tcacggaggt
      121 ccgggggcgt gacgagcccg accagcgggt gatcggcacg tatccggacg agatcagtgc
      181 ggtggaggcg gtccggtcgg cgaagacaga gttcgtcgag tcgggttcgg aggatttcgc
      241 gtggtgggtg gtccgtcaac caggagccac cctggcccaa ttcatctccg attccaggag
      301 cgacaaggag ttcgtcctcg atctgaccac gggtcagctg atcgaggtct gaaagcgccc
      361 gcgccaacgc catctctacc cttcgagcgt ggctgaatcg cctgtttccg atacggagcc
      421 ggtgcgaacc gcggaagtga tcgcagccgc ctgtctggcg accgacctgg ggatggggtt
      481 cccgttcgag cacgggttgc aggcgacttt ggttacgtgt cgtctcgctg agcggctggc
      541 tgtcggcccc aagacagccg cagaggcgtt ctacgtcagc cttctcatgt attcgggctg
      601 caccaccgat tccgacatcg ccactggcat cttcggtggg agcttgacca ggaacatgac
      661 cgctcgacag ttcgggtcga cgctcgaggc catgggagga gtgatacgcg ctctacccga
      721 ccccgactcg ggcattttca agagtggcct cgagatcgcc gccaacctcc ccaaggcggc
      781 catgttccgt agcacccatt tcaccgcctt gtgcgaggtc gccatgatgt tgtcggaacg
      841 cctcggtctc cccgacgaga tcaatcggct cttcctctac ctcaccgagc gttgggacgg
      901 caagggtgtc ctgggccgaa aggccggtga cgacattccc atcgcgttga gaatcgtgac
      961 ggtggcgagg gatgccgcct atcagaccct gatgggaggg gaggagcacg cggcccgggt
     1021 gatcaaggac cgggccgggc atgccttcga ccctgatgtg gccgaggcgc tctcgcacaa
     1081 cgcctcggag atgctttcga ccgacgaaga cggcccgtcg gtgtgggact cggtcctcga
     1141 tgccgagccc gggccgcacc tcacactgga ctcgggagcg gtggaccgag cccttggcgc
     1201 cctcggcgac ttcgccgatc tcgtctcccc gtcattggtg ggtcattcga ccggcgtcgc
     1261 ccggttggcg ggcgaggccg caagcgacct cggaatgacg ccggaggaag tgaccaacat
     1321 tcgaagggcg ggtctgatcc atgacatcgg ccgagtggcg atccagcccc ggacatggca
     1381 gaagaacagt tcgctcaatg ccgacgaatg ggaacaggtg cgcttgcacg cctatcacac
     1441 cgagcgggtc ttcaaccgat caccgtttct ggcccctgtg gcggccgtag cctgccgaca
     1501 ccatgagcgg atggacgggt ccgggtatca ccgcggcctg aatgccacgt ccctggagcc
     1561 ggcatcgcgt ctgctcgctg ctgccgacgc cttccatgcg atgacggaac cacgcgacca
     1621 tcgccccccg ttgccgctcg ccgatgccgg cgaagtgctt cgcgtggaat ccgccgcagg
     1681 tcgccatgac ggcgagatgg tcgctgcggt gttgcgtgcc gccggtctgc agacatctcc
     1741 ggtggagcgc cccgctggtc tcaccgagcg cgaagtacag gtgatccggc tcctggcccg
     1801 tggccttcaa accaagcaag tggcgcgcac gttggacatc tcggccaaga cggccgaccg
     1861 ccacatccaa aacgcctacc ggaagatcgg tgtctccacc cgggccgcag ccacgctcta
     1921 cgccagcgag cacggcctgg tgcaatgggg agaactcccg atttcgctct gatccccctc
     1981 gtcgtacggt cgatgccatg tcgagggtgc atggtgatcg aggccgggca gccgccaaac
     2041 cgctgatcgc agccggcgca ctcagcctgg ctctctcgat cggactggtg gcgacttcga
     2101 cagcgagcac cacgaccctg ggggcaccgg cttggtggcc atggctcctc accggccttc
     2161 aggtgctcgc cctgtgggcc gcggggcgcg gctggtggtg gggatggctc ctcggaggag
     2221 ccgtgcagcc ggtgtggatc gcctacgcct tgaccaccgg ccagctcgga ttcgtcccgg
     2281 gatgcattgt ctcttcctgg gtgcagaccg ccagcttcat gcgacagaag accgaaggag
     2341 aggatcatgc cgacaagtca agagcgaatc ccgaccgtcg tcatcggagg cggccaggcc
     2401 gggctttcgg ttggatatca cctgcgacgg ctggcggtgc cattcgtcat cctggacgcc
     2461 caggaaagag tcggtggggc atggcggggc cgatgggact ctctccgttt gttcactcca
     2521 gccaggtttg acggcctgga tggcaagcca ttccccgccc ccagtcatgc attccccacc
     2581 aaggacgaga tggccgacta cctggaggcg tattcggacg agttccacct cccggtccga
     2641 agcggccaga gggtgaagag tcttacgaaa cgggactcgg tcttcatcgt ggagacggac
     2701 gacgtccgtt tcgaggctca aagcgtggtg gtggccatgt ccagctggca ggtgccacgc
     2761 atcccgggct tcgccaaaga gttgagaccc gacatcgtgc aaatccacgc tgcggattac
     2821 cggaatcttt ctcagttgca ggaagggccg gtcctcgtgg tcggcgccgg caactcaggg
     2881 tcggagatag cgatcgacgt cgtcggctcc cacgaaaccg tggtcgccgg gcccgacacc
     2941 ggccacgtcc cgttccgggt ggaaggccgg gtcgggcggc tgctcgtccc tgtcgtccgg
     3001 ttcgtgttcc atcgcgtcct aaccaagagc aaccccatcg cccggcgaag gatgccgaag
     3061 atgatcgcct ccggcgagcc cttggtgcgg acgaagcctc gcgatctcat ggaggcgggc
     3121 attgagcggg cgccgcgagt cgtcggtgcc gaggacggct tgcccgtgct cgaagacggt
     3181 cgcgtcatcc acgccgccaa tgtcatctgg tgcacagggt tcgagcctgg cttcacctgg
     3241 atcgatctac ctgtgttcga agatggtcgc gtcaaacacg atcgaggggt ggccgacgtc
     3301 ttcggcctct acttcgtggg cctcaaaggt ctcttttcgg tgtcctcggc ccaggtgaat
     3361 ggtgttggcc gtgatgccga gcgggtagca aagctgatcg ccgccacgat gtcgccggcc
     3421 cccgcccctg ccggggcgcg gcggtgagag cggatcgggt cttggtggtg ggcggcggac
     3481 tggccggcct gagtgcgggt atcgcgctgg ctcgcgccgg ctctcatgtg gagatcgtgg
     3541 agaaggccga ccagtgggcg gacgtcggcg ccgggatgta cctgcccggc aacgcacacc
     3601 gggcactgga gtcgctgggc atcggcacgg tgatgcgaga tcgggcgttc cagatcaaac
     3661 accagatctt cagcgatcac gccggccgga cgttggccga cgtcgacctg caccaggttt
     3721 gggtaggcgt gggcccgtgc cttgcgatcg cccgcgccga cttgcaccgt gctctgcggg
     3781 acgcggcacg cggcgtcgac atgcgtatgg gtgtcgaggt cgagtcgctg gagcaggatg
     3841 ggggctctgc agtggtcgat ttcaccgacg gaagcagtgg cgaattcgat ttgctggtgg
     3901 gagccgacgg cctcaactcc tcggtccgca gacacatgtc ggggaagtct gagccgagcc
     3961 cggtaggcca ggtcagctgg aggttcatca cgactggtga cgccgggctc agcaactgga
     4021 ctgtcatgct cgggggcgag aagacgttcc tggcggtccc cctcggcgga ggccgcgtgt
     4081 actgctacct cgacgtgtcg agtcacgacg gtgccgaccc gaccgaaggc gacccgggcc
     4141 gtctcgagga gatgttcggc gatttcgccg ccccggccac cgccttcatt cgggcagcaa
     4201 cggagagtgg ctccgtccat ttcggagtca tcgaagaggt cgtcgccaac gattgggtga
     4261 ccgggcgggt cgttctggtg ggtgatgcgg cccatgccac gtcaccgaac atggcgcaag
     4321 gagccgccat ggctttcgaa gacgccgtag tcctt
//
LOCUS       DASTXO010000026         2524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_995972, whole genome shotgun sequence.
ACCESSION   DASTXO010000026 DASTXO010000000
VERSION     DASTXO010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995972"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VFU96_00495"
     CDS             <1..855
                     /locus_tag="VFU96_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5111758.1"
                     /translation="RDPSIDVIVTAAVPDQRSAIAVEALTNRKDVVADKPGCITLDQL
                     DEIEKAVTQSGRFWSVTFSERFEVRCASKAGQLVREGRIGRVVQTLGLGPHREGDRAH
                     IAGGEGRPDWFYNRDRTGGIITDIASHQIDQFLWFTGAENAEIVSSTVANYTHPELPK
                     MQDFGEVTLRADGAHGYVRVDWYTPQGLPTWGDGRLVILGTEGYIELRKYVDVAGRAG
                     RDHLFVVDSAGTEYINCSDVELSYYPDLVHDVINRTESACPQRHTFEVMRLAIRAQQS
                     ATMLGFAE"
     gene            852..2060
                     /locus_tag="VFU96_00500"
     CDS             852..2060
                     /locus_tag="VFU96_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5111759.1"
                     /translation="MNGSAEEKRLRVGIVGCGMIARSHAMSYQDNPHVELVGVVDVDP
                     VRAAAFAADYKTTAYGSCRELLDQQPGLASVATPPGNHTEVAVELLDSGCSVLLEKPP
                     TTTLADMDVLADAEKLSSGSVYVVFQHRHGSGARRAHHLLHSGALGTPRVAVCETLWY
                     RPDDYFLPEWRGNWAGEGGGPTLGHGIHQIDLLLHLLGPWETVDGRAARIARPVEFED
                     VSVAILTFSSGAVGTVVTSLLSPRELSRIRVDTTGGTLEVNHVYGYSDADWSWTPAPD
                     ATTAASLGRDPGLPDTGSTGHPGPAGSADIWAASAGLDLPSNHAAQIDQLVDDMLVGR
                     PHETTLASTRPTMEFITALYASSLERETVHRRDLTPADGFYHSLNGGLSADTITARLG
                     AAPDQGPRHH"
     gene            2155..>2524
                     /locus_tag="VFU96_00505"
     CDS             2155..>2524
                     /locus_tag="VFU96_00505"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU5111760.1"
                     /translation="MLPALLLLVCLVAAAACAPGQSQRSESDSGGGATLRVSTWGNDS
                     RVRLTQQAVDAFTAANPDVKVTIENTDWGGYWDKLATMTAGDNSPDVIQMDESYIAAY
                     GARDALLDLGTVKDNLDLSAM"
BASE COUNT          465 a          847 c          792 g          420 t
ORIGIN      
        1 cgcgatccct cgatcgacgt catcgtgaca gccgcggttc cggaccaacg ctccgccatc
       61 gcggtcgagg ccctgacgaa ccgcaaggac gtcgtcgccg acaagcccgg ctgtatcacg
      121 ctggatcagc tcgatgagat cgagaaggcg gtcacacagt ccggtcgatt ctggtcggtc
      181 actttctcgg agcgcttcga ggtgcgctgc gcgagcaagg ccggacagtt ggtaagagag
      241 gggcggatcg ggcgggtcgt acagaccctg ggacttggac ctcaccggga gggtgaccga
      301 gcccacatag ccggtggcga gggacgtccg gactggttct acaaccggga ccgaaccgga
      361 ggcatcatca ccgatatcgc aagccaccag atagaccagt tcttgtggtt caccggcgcc
      421 gagaacgccg aaatcgtctc gagcaccgtc gccaactaca cgcatcctga gctgccgaaa
      481 atgcaggatt tcggtgaggt gacgctgcgg gccgacggtg cccacggcta cgtccgcgtc
      541 gactggtata ccccgcaagg cctgccgacc tggggagacg gccggctcgt gatcttgggc
      601 accgagggct atatcgaact ccgcaagtac gtcgacgtgg cgggccgcgc gggcagagat
      661 cacctattcg tggtcgactc agccggcacc gagtacatca actgttcgga cgtcgaactc
      721 agctactacc ccgacctggt gcacgacgtg atcaaccgca ccgagtcagc ctgcccccag
      781 agacacacct tcgaggtgat gcggctggca atccgcgcgc agcagagcgc gacgatgctg
      841 gggttcgccg agtgaacggg tcggcggagg agaaaaggct ccgtgtcggg attgtcggat
      901 gcgggatgat cgcccgttcg catgccatga gctaccaaga caacccacac gtcgagttgg
      961 tcggcgtagt cgatgtcgac ccagtacgcg ccgctgcgtt tgccgccgac tacaagacaa
     1021 cggcgtacgg ctcctgccgc gaactcctcg atcaacagcc tgggctcgcg tccgttgcca
     1081 ccccgcctgg gaatcacacg gaggtcgctg tcgagctcct cgattcgggc tgctcggtgc
     1141 tcttggagaa gccaccgacc accaccttgg ccgacatgga tgtcctcgcc gacgctgaaa
     1201 agctgagctc ggggtccgtc tatgtggtgt ttcagcaccg tcatggatcg ggcgcgcgtc
     1261 gcgcccacca tctgctgcat tcaggagccc tgggaactcc gcgggtagcc gtctgcgaaa
     1321 ccttgtggta ccgccccgac gactacttcc tgcccgagtg gcgtggcaat tgggcaggcg
     1381 agggcggcgg gcccaccctc ggacacggca tccatcaaat cgacctgctg ctccacctct
     1441 tgggcccgtg ggagaccgtc gacggtcgcg cggcgcggat cgcacgtccc gttgagttcg
     1501 aggacgtgtc ggtggcgata ctgacgttca gcagtggcgc agtaggcacg gtcgttacca
     1561 gcctcctctc cccgcgagag ctcagtcgca ttcgggtgga taccaccggt ggcaccctcg
     1621 aggtcaatca tgtctacggc tacagcgatg ccgactggtc ctggacgccc gcgccggatg
     1681 cgacaacggc agccagtctt gggcgtgatc ccggtctgcc ggacaccgga agcactggac
     1741 atccggggcc ggcaggcagc gctgacatct gggccgcctc cgctgggttg gatctgccca
     1801 gcaatcatgc ggcccagatc gatcagttgg tcgacgacat gctcgtcggc cgcccccatg
     1861 agaccacgct ggcaagcacc cgcccgacga tggagttcat caccgcgctc tacgcctcct
     1921 ctctcgagcg cgaaaccgtt caccgacggg atctgactcc ggccgacggc ttctaccaca
     1981 gcctcaacgg cggtctgtct gccgacacga tcacggctcg actgggagcg gctcctgatc
     2041 agggccctcg ccaccactga cgcagatgac tcgccaactt tcgaccaccc cccaacaccc
     2101 atgaaaggca ccacgatgac ctccctccga ttgcgatcgc aaggacttcg tcgattgctc
     2161 cccgcgctcc tgctgctggt ctgcctcgtc gccgcggccg cgtgcgctcc gggccagagc
     2221 cagaggagcg agagcgattc cggcgggggg gccacgctgc gcgtctccac atggggcaac
     2281 gactcccggg tcagactcac ccagcaggcg gtggacgcct tcaccgcggc gaacccggac
     2341 gtcaaggtca cgatcgagaa caccgactgg ggcggctatt gggacaagct cgccacgatg
     2401 acggcgggcg acaactcgcc tgatgtcatc cagatggacg agtcctatat cgccgcctat
     2461 ggcgcccgag acgccctcct cgacctaggc acggtgaagg acaatctcga cctgtcggca
     2521 atgg
//
LOCUS       DASTXO010000027         5260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_664458, whole genome shotgun sequence.
ACCESSION   DASTXO010000027 DASTXO010000000
VERSION     DASTXO010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664458"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VFU96_00510"
     CDS             <1..835
                     /locus_tag="VFU96_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEU5111761.1"
                     /translation="YRVTCRPRGVYRVGPTMARVSDPLGLAETSAWTGPADRIVVYPA
                     VEELNGFPIVRGQDPAMQASRPEHARRGGEDFYTLREYQQGDDLRRVHWPSSARTDQL
                     MIRQLETPWQSRALVLLDVRSTSYESQDAFEKAVSGAATIVTHLVGSGFDADLWAGDA
                     EAIDAARYSSAMERLALVQPDPAIDIEAVAGRIRQKGGGGALVIVTGVADRPLLGVQQ
                     LLAGDYPTTVLMTASASTSQTLVGFHRMGVTTVSVAPDQPWAPAWVIAMKSSWTTASV
                     G"
     gene            811..3129
                     /locus_tag="VFU96_00515"
     CDS             811..3129
                     /locus_tag="VFU96_00515"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HEU5111762.1"
                     /translation="MDNGLGRLAGAAAFVLMLVRLGRLLDTGPEAPDWQLIMLASAFL
                     GGVIWWLLGQTVNNKRVIMALFSVAGLALFLRIAVADTLFWGFLPTPGTLGALADEMA
                     QTLDLVRFGVAPVFPTSGVVAVLALLMWITGALYVWGATTGPSIAMIAPSLGLYLQFA
                     VMDRIPGGPVWMGAVAVMIALALASIGIERRQDAGRVRDVDGRPLARRAGGMALILAA
                     VVAGGSLLVADSASSLVPPTGNVRWRYGGGYGPGFGGVTFDRLADLHQSIIRRTNAVV
                     FRATLDPNAPPANEIYWRMESLDFFDGTAWRPSANRADFYQPGIGGGDPQHAYRGTSQ
                     EITQRVQIVDLRSQVLPTAGVGQFFRSESVDVSGFQITPDGSAIFQAELDEGDEYEAQ
                     SILPLQDEDLGALATLPDGTLSPLFANAAEAGATSIEPGEPPGDVTPPDDITRFTDFN
                     EEVPRALALLARRQTAGASTPFEAAWLLQHWFRDSNEFTYSTDVSTGHDTLDLEAWLT
                     DPNSPNAKIGYCEQFAAAMAYLGRVVGIPSRVVWGFTPGTVEQQDDGTEVIVVRDNNA
                     HAWVEMWMDGFGWVRFDPTPRADGTLPESVTAEFDPEPYLPERGEDASTLGQPGFFAE
                     SQFEEGGGAIDRSAAANTGFRLTWVWLVLPVLAILIALVPLLKAVRRRRRIKLLHQGD
                     ITAAWDEIVDRLADLGSPVPSHQTPLEFAHATDRSLVPLASSYSATVYGDRQGLGHVS
                     DLTHVETWIKRNFEGRDRALASLNPKSLIDRD"
     gene            complement(3131..3628)
                     /locus_tag="VFU96_00520"
     CDS             complement(3131..3628)
                     /locus_tag="VFU96_00520"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU5111763.1"
                     /translation="MDQSFTAEQACRLTNCTHHQLRYWDRVGLVKPSVQTTGGRPGVR
                     RLYSFRDLVALRVVRSLLDNGMSLQRVRRAWDYLRREGEMDDHLAEVKLVTDGQTIFH
                     VGSDEGELMDALRTGQLAFFVAIDEITREVEEDVSRFELDRSRFLDMLRRVEDDISDE
                     RAAEG"
     gene            complement(3745..4080)
                     /locus_tag="VFU96_00525"
     CDS             complement(3745..4080)
                     /locus_tag="VFU96_00525"
                     /inference="COORDINATES: protein motif:HMM:NF022684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HEU5111764.1"
                     /translation="MALDDHEQKILAEIERQFYEEDPELARAVREIAKPSRIGVRLAV
                     LGVVAGLAIVIGFFASNTVVAFLGFGLLVGSAAALVTGLRSRAWRATESEPQGEEHGP
                     GWQDPFRRD"
     gene            complement(4152..>5260)
                     /gene="dinB"
                     /locus_tag="VFU96_00530"
     CDS             complement(4152..>5260)
                     /gene="dinB"
                     /locus_tag="VFU96_00530"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU5111765.1"
                     /translation="DPGLVGKPVAVGGTGNRGVIASASYEARAYGVRSAQPTVTALRL
                     CPDLIIVPPSHGRYGDLSAGVFDVFRGFTPHVEGLSLDEAFLDVAGLRRHYPTSTDVA
                     IAVRREIRSRLGLPASVGIASSKFMAKLASEAAKPDGYRHIPKHAEVEFLHALPVEAL
                     WGVGPATLAGLQRLGVVTIGDLAELPEAAVLGSLGPTQGRHLLDLARGIDHRPVMSDI
                     EAKSISVEETYDRDLEGAEVIETALLALAQRMADRLHRSGLAARTVTLKLRFADFTTV
                     TRSVTSGTVIDGPREIYVTALALLGHVAIDRPVRLLGLGGSGLEPAGEPRQMPIGAED
                     NWSRVDEAVAGVRERFGEHSVEPARLLRSHEHDL"
BASE COUNT          910 a         1754 c         1659 g          937 t
ORIGIN      
        1 ctatcgggtc acctgtcggc ctcgcggcgt ctaccgggtc ggtccgacga tggcgcgggt
       61 gagcgatccc ctcggtctgg ccgagacgag cgcgtggacc gggccggcgg acaggatcgt
      121 ggtgtatccc gcggtcgagg agctgaatgg cttccccatt gtccgaggcc aggatccggc
      181 gatgcaggcg tcccgtcccg agcacgcccg gcgcggcggg gaggacttct acacgttgcg
      241 tgaatatcag caaggagatg acctgcgccg ggtccactgg ccatcgtcgg cgcgaaccga
      301 tcagctgatg atccgtcagc tcgagacacc gtggcagtcc cgagccttgg tcctcctcga
      361 cgttcgtagt accagttacg agtcacaaga cgctttcgag aaagctgtat ccggcgcggc
      421 gacgatcgtc acccacctgg tcgggtccgg gttcgatgca gacctatggg ccggagacgc
      481 agaggcgatc gacgctgccc gctactcgtc ggccatggag cgactggcac tcgtgcagcc
      541 cgatccggct atcgacatcg aggcagtcgc gggtcggatc cggcagaaag gtggtggtgg
      601 ggcgctcgtg atagtcaccg gcgtggccga tcgcccgctc ctcggagtgc agcagcttct
      661 tgccggcgac tatcccacga cagtcctcat gacggcgtcg gcctccacct cgcagaccct
      721 ggtcgggttc caccgcatgg gggtgaccac ggtctctgtc gcccccgacc agccctgggc
      781 tccggcctgg gtgatcgcaa tgaagagctc atggacaacg gcctcggtcg gctagccggc
      841 gccgccgcct tcgtgttgat gctcgtccgg ctcggacgtc tactcgacac ggggcccgag
      901 gcgcccgact ggcagctcat catgctcgcc tccgccttcc tgggcggtgt gatctggtgg
      961 ctgctgggtc aaacggtcaa caacaagcga gtgatcatgg ccctcttctc cgtggccggt
     1021 ttggctctct tcctccggat cgcggtcgcg gacacgctct tttggggctt tctccctacc
     1081 cccggcacgt tgggggcact cgctgatgag atggcccaga ccctggacct ggtccggttc
     1141 ggtgtggcgc ccgtgttccc cacttctggt gtcgtcgcag tcttggccct gctgatgtgg
     1201 atcaccggag ctctatatgt ctggggtgcc actacagggc cctcgattgc gatgatcgcc
     1261 ccttcgctcg gcctctacct ccagttcgcg gtgatggacc ggatccccgg tggcccggtg
     1321 tggatgggag cagtagccgt gatgatcgcc cttgcgctgg cctcgatcgg catcgagcgg
     1381 cgacaggacg ccggtcgagt ccgtgatgtc gatggacggc ccctggccag acgggcgggt
     1441 ggcatggctc tgatcctggc cgccgtggtg gcgggagggt cgctgctcgt cgccgacagt
     1501 gcgtcgtcac tggtcccacc gacagggaac gtgcgctggc gctatggcgg cggttatggg
     1561 cctggtttcg gtggggtgac attcgatcgc ctcgccgacc tgcatcagag catcatccgg
     1621 cgcaccaacg ccgtggtctt tcgagccacc ctggacccca atgcgccccc cgccaatgag
     1681 atctactggc ggatggagag cctcgatttc ttcgacggaa cggcgtggcg cccctccgcc
     1741 aaccgggccg acttctacca accgggtatc ggtggcgggg acccgcaaca cgcctaccgg
     1801 ggaacgagtc aggagatcac acagagagtc cagatcgtcg atctcaggtc gcaggtcctg
     1861 cccacggccg gcgtgggtca attcttccga tccgaatctg tggacgtcag cggattccag
     1921 atcacgccgg acggctcggc gatcttccag gccgagctcg acgagggcga cgaatacgaa
     1981 gctcagtcga tcctccccct ccaggacgag gatctcggcg ctctcgccac gttgcccgat
     2041 ggcactctca gcccgttgtt cgccaatgca gctgaggctg gggcgacctc gatcgagccc
     2101 ggtgagcctc ccggagacgt gactcccccc gacgacatca cgagattcac cgatttcaac
     2161 gaagaggttc cgagggcgct ggctcttctg gccagacgtc agactgccgg ggcctcgacc
     2221 ccgttcgagg ctgcctggtt gctccagcac tggttcaggg acagcaatga gttcacctat
     2281 tccaccgacg tgtcgaccgg ccacgacact ctcgacctcg aggcctggct caccgacccg
     2341 aacagcccca acgccaagat cggatattgc gagcagttcg ccgccgccat ggcctatctg
     2401 ggtcgtgtcg tgggcatccc gagccgagtg gtctggggct tcaccccggg gaccgtcgaa
     2461 cagcaggacg acggtaccga ggtgatcgtg gtccgtgaca acaacgccca cgcttgggtc
     2521 gaaatgtgga tggacggatt cggctgggtg cgcttcgacc cgacgcctcg cgccgacgga
     2581 acactgcccg agagcgtcac cgccgagttc gatccggagc cctacttacc cgagagaggg
     2641 gaggatgcga gcaccctcgg tcaacccggg ttcttcgcag aaagccagtt cgaggagggt
     2701 ggaggggcga tcgaccgcag tgccgccgcc aacaccggct tccgattgac gtgggtctgg
     2761 ctcgtgttgc ccgtcctcgc cattctcatc gcgctcgtgc cattgctcaa ggcagtccgg
     2821 aggagaagac ggatcaagct cctccaccag ggtgatatca ccgcggcatg ggacgagatc
     2881 gtcgatcgtc tcgccgacct cggctcaccg gtgccctccc atcagacccc gctcgaattc
     2941 gctcacgcca ccgatcgatc tctggtcccg ctcgcttcgt catattcggc cacggtttac
     3001 ggggatcgcc aaggtttggg ccacgtctca gacctgaccc acgtggaaac gtggatcaag
     3061 cggaatttcg agggtcggga tcgggccctg gcctccctga acccgaagtc gttgatcgac
     3121 cgggattgac tcaaccctcg gcggcgcgct cgtcgctgat gtcgtcctcg acccggcgaa
     3181 gcatgtcgag gaaccgactg cgatcgagct cgaaccggga gacatcctcc tcgacctcac
     3241 gcgtgatctc gtcgatggcc acgaagaacg cgagctgacc cgtgcgcagg gcgtccatca
     3301 actccccttc atcggagcca acgtgaaaga tggtctgccc gtcggtgacg agcttcacct
     3361 cggcgagatg gtcgtccatc tccccctcgc gacggagata gtcccaggcg cgtcggaccc
     3421 tctgcagcga catgccattg tcgagaaggc tgcgaacaac tctcaatgcg acgagatcgc
     3481 ggaacgagta gaggcgacgc accccgggtc ggccacccgt tgtctgcacc gagggtttga
     3541 ccagtcctac acgatcccaa tatcgaagtt gatgatgagt gcagttcgtc agccgacatg
     3601 cctgctcggc tgtgaatgac tggtccacgc gtcccttcct aataccaact gccccgaccc
     3661 agagtcatgg gccgacgctc tcctcctcag gtggtggaac ctcccgagcc caccgccagg
     3721 ggcgggtaca ttagccgctc ggagtcagtc gcgacgaaag ggatcctgcc agccgggacc
     3781 gtgctcttca ccctgcggtt cgctctccgt cgcgcgccag gcccgggacc gaagcccggt
     3841 gaccagggcg gccgctgagc caacgagcaa cccgaagccg aggaatgcga caacggtgtt
     3901 cgatgcgaag aagccgatca ctatggcgag accggcgacg actcccagca cggcgagccg
     3961 cacaccgatg cgcgacggct tggcgatctc cctcacggcc cgtgccagct ccggatcttc
     4021 ctcgtagaac tgacgctcta tctccgccag gatcttctgt tcgtgatcat ctagggccat
     4081 gtcaacatct caacatccgg taacagccct ctgacacaat acacccgggc tggagacagt
     4141 tcaccgatga atcaaagatc gtgctcgtgg cttctgagga gacgggccgg ctccaccgaa
     4201 tgttccccga atcgctcccg cacgccggcc acggcctcgt cgacgcgaga ccagttgtcc
     4261 tcggcaccga tcggcatctg cctcggctca ccggctggtt ccagacccga ccctccgagc
     4321 ccgagcaaac gcacgggacg atcgatcgcg acgtgcccga ggagagctag ggccgtcaca
     4381 tagatctcgc gtggaccgtc gatgactgtc ccggatgtga cgcttcgggt cactgtcgtg
     4441 aagtcggcaa atctgagctt caaggtcacg gtgcgcgcgg cgaggccaga tcgatggaga
     4501 cggtcagcca ttctctgggc tagagctagc agggccgtct cgatgacctc cgcgccctcc
     4561 aggtcgcggt cgtaggtttc ctcgaccgat atcgatttcg cctcgatatc ggacatcacg
     4621 ggccgatggt cgatgccacg ggcgagatcg agcaggtggc ggccctgtgt tgggcccagc
     4681 gaccccaaga ccgcagcctc gggaagctcg gcgaggtctc cgatcgtcac cacaccgagc
     4741 cgctgaagcc cggccaacgt ggccggaccg acgccccaca acgcctccac ggggagcgca
     4801 tgcaggaatt ccacctcggc gtgcttgggt atgtgtcgat atccgtcggg cttcgccgcc
     4861 tccgacgcca gcttggccat gaacttcgag gacgcgatgc caaccgaggc gggaagaccg
     4921 agacggctcc tgatctcccg ccggactgcg atagcgacat cggtcgatgt cggataatgg
     4981 cgacggagcc ccgctacgtc caggaaggcc tcatcgaggc tcagaccttc cacatggggg
     5041 gtgaagcccc ggaagacgtc gaagactccc gccgagaggt ctccataacg cccatgagag
     5101 gggggcacga tgatcaggtc cgggcacaga cgcagggcgg tcaccgtcgg ttgggcggat
     5161 cggaccccgt aggcgcgcgc ctcgtaggag gcactggcga tcacccctcg atttccggtc
     5221 cccccgaccg cgaccggttt tccgaccaga cccgggtctt
//
LOCUS       DASTXO010000028         4546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1806524, whole genome shotgun sequence.
ACCESSION   DASTXO010000028 DASTXO010000000
VERSION     DASTXO010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806524"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VFU96_00535"
     CDS             complement(<1..560)
                     /locus_tag="VFU96_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111766.1"
                     /translation="MTLTPANLRAFDLLVDTILPAVPGDGAAWTTPGEDLRLSAGLPG
                     IYDALPHERDRKDLGLFLRLLDSAAGGLVLFGRPSNFTNLAPDQRAAALLAMERNRVE
                     IVRKGVRALKTLAAFLWVTTDDPSRPPPAWAAMRYPGPDGPPSMAIKPIQTEAVDRDT
                     TIDCDVVVVGSGAGGGIAAALLAAAGL"
     gene            554..1207
                     /locus_tag="VFU96_00540"
     CDS             554..1207
                     /locus_tag="VFU96_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111767.1"
                     /translation="MSPLESYLHGSSGSFVAEVDRDALQHGVFIPVVIDAERVVDGAE
                     MVRVTYFAVTTDAPAAVWEVELPSVAQLHGIGALGLAGVIYSAMRESRGPGPETVEFD
                     LFTDADDIAAVFALEESGMPLTVQLEDVWMPRIWFEDEPARGDVYRVALEPFQDSYRY
                     TAGTIAPDVLLELDVSGQVISSPVETEAVKQWSQDQIDASRRAFPDKEVKLRWRDDG"
     gene            1200..2060
                     /locus_tag="VFU96_00545"
     CDS             1200..2060
                     /locus_tag="VFU96_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5111768.1"
                     /translation="MAELAVTIIDVGWGDAILVDSRDDGGDRRFGLIDCNDYDRQRTA
                     LPFVKRYFERLGRDWQATQHNFEWVLLTHGHADHARGLKEMMNTFGTTHFWYPKSVAS
                     TTYGVLLDFANRSNRVLHHQSVDETKILEEPEVTFHCGMRILWPNFDQIDTANENNNS
                     VVLALTLGEVTIVLTGDAEAENWPEIVDRLPSHTTYLQVPHHGGRNGLFDPNNGTPWL
                     DSLNPRRTRLVMSSHVVPHGHPHPDVVTELADRGFVSYRTDRNYHLTLATSGERVSVG
                     YTHVSGRLPR"
     gene            2199..3140
                     /locus_tag="VFU96_00550"
     CDS             2199..3140
                     /locus_tag="VFU96_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEU5111769.1"
                     /translation="MATKSLEKLSKTELYERAQEADVPGRSQMDKDELIEALSDGGNG
                     TSKGRSPKSKRSIWNGAITFGLITIPVGLYTATEDKDISFHLLSGKDESRIQYKRVST
                     KTGREVDWDDIVKGYEYEKGKYVVFTPEELEQIAPDSARSVDVVQFVDASEVDPIFFE
                     KSYYVVPTDVAVKAYQLFVRALEESGRVAVAKVAIREKERLCTLRVRDGSLVLETMKW
                     PDEIRDADFEQLDKRTRLSAQELKMARQLIDQLTGKFDPEVFEDSYRQRVEEAIEAKV
                     EGNEITVTPAAAPTEKVVDLLEALKSSVEQTKTRRSA"
     gene            3137..3379
                     /locus_tag="VFU96_00555"
     CDS             3137..3379
                     /locus_tag="VFU96_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111770.1"
                     /translation="MTLGDRPADDPSRPIEEWSDEELVAQFRSIKGELTSEAPGSWDL
                     DGAPALVIEEEMKRRGLTPDREDLIPDAGPDPDTPV"
     gene            3431..>4546
                     /locus_tag="VFU96_00560"
     CDS             3431..>4546
                     /locus_tag="VFU96_00560"
                     /inference="COORDINATES: protein motif:HMM:NF016196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HEU5111771.1"
                     /translation="MTTGPSIGLTAYEERRVEELRRWKRRANGFLLGALVVFLVSHFF
                     GGETGINGFIRAASEAALIGGIADWFAVTALFRHPLGIPIPHTALIPRSKDEIGRGLA
                     EFVQQNFLDPANLRNWLMNADIAGRLAQWLASSDHATLAARRTIETGSTIAQSLDDER
                     VATVVAQTSLDWLRQTPITPIISLVVDAWMKGGRTLQSVEATLRGLDVVIVDNLPYFR
                     DSFAKTSPWWMPAWAGELVFERIIASFRLLVDEVASDPSHPIRADIEMMLSRIADDLR
                     NSADLAERIEESKLHILDSPELAAAVQTQWQVVQESLGRAASQPGSDLEKRLSELIQW
                     WANRVLEDEALRMRIDTWIAEAAERLAHQWDDEVIGMI"
BASE COUNT          873 a         1397 c         1486 g          790 t
ORIGIN      
        1 agtccggcgg cagcgagcaa tgccgccgcg attcctccac cagctcccga tccgaccacg
       61 acgacatcac agtcgatggt cgtgtcccga tccactgcct cggtttgtat cggcttgatc
      121 gccatcgagg gagggccatc cgggcccggg tatcgcatgg cggcccaggc cggaggcgga
      181 cgggacggat cgtcggtggt gacccacagg aaagcagcca gggtcttcaa ggctcggacg
      241 cccttgcgga cgatctccac ccggttgcgt tccatggcga gcagggcggc agcccgctgg
      301 tcgggggcca ggttggtgaa gttcgacgga cggccgaaga gcaccagacc accggcggcg
      361 gagtcgagga gacggaggaa cagcccgaga tccttccgat cgcgctcgtg gggaagtgcg
      421 tcgtagatgc cggggagtcc cgccgatagc cggagatcct cgccgggggt agtccaggca
      481 gcgccgtcgc cagggacggc cgggaggatg gtgtcgacca agagatcgaa agcacggagg
      541 ttggccgggg tgagtgtcac ccctcgaaag ctaccttcac gggtcgtcgg gtagctttgt
      601 cgccgaggtg gatcgggacg cccttcagca cggtgtgttc atccccgtgg tcatcgacgc
      661 cgagcgggtc gtcgacgggg cggagatggt tcgggtcacc tatttcgccg tcaccacaga
      721 cgctccggcg gcggtctggg aggtcgagct tccaagcgtt gcccagctcc acggcatcgg
      781 agctctgggt ctcgccgggg tcatctacag cgcaatgcgg gaaagccgag ggccagggcc
      841 ggaaacagtg gagttcgacc tgttcacgga tgccgacgac atcgctgccg tattcgccct
      901 cgaggaatcg ggcatgccgt tgacggtgca attggaggat gtgtggatgc cacgcatctg
      961 gttcgaggac gagccggccc gcggtgacgt ctaccgggtg gcactcgagc ccttccagga
     1021 ttcatatcga tacacggcgg ggaccatcgc cccggacgtt ttgctggagc tcgacgtgag
     1081 cgggcaggtg atcagctcgc cggtcgagac cgaggccgtg aagcagtggt cacaggatca
     1141 gatcgacgcc agcaggcggg ctttcccgga caaggaagtc aaactgaggt ggagggacga
     1201 tggctgagct cgcggtcacc atcatcgatg tcggctgggg agacgcgatc ctggtcgatt
     1261 cgcgagacga tggtggcgat cgtcgcttcg gcctcatcga ttgcaacgac tacgaccgtc
     1321 agcggacggc gcttcctttc gtcaagcgct atttcgaacg gctgggacgg gactggcagg
     1381 cgacacagca caacttcgaa tgggtcctgc tcactcatgg acacgccgac catgcccgtg
     1441 gcctgaagga gatgatgaac accttcggga cgacccactt ctggtatccg aagtcggtcg
     1501 cctcgaccac ctacggcgtc cttctcgact tcgccaacag gtcgaaccgg gtactccatc
     1561 atcaatcggt cgacgagacc aagatcctgg aggagccgga ggtcaccttt cactgcggga
     1621 tgcggatctt gtggcccaac ttcgaccaga tcgacactgc caatgagaac aacaactcgg
     1681 tcgtgctcgc cctgactctc ggtgaggtca cgatcgtgct caccggcgac gccgaagccg
     1741 agaactggcc ggagatcgtg gatcggctgc cgagccatac gacctatctc caggtcccgc
     1801 atcacggggg gcggaacggg ctattcgacc ccaacaatgg gacgccatgg ctcgacagtc
     1861 tcaacccgag gcggacgcgc ctggtgatga gcagccatgt cgttcctcac gggcaccctc
     1921 accctgacgt ggtcaccgag ctggccgacc gcggcttcgt ctcgtaccgg accgaccgca
     1981 attaccacct cactctcgcc acatccgggg agcgggtctc ggtcgggtat acccatgtca
     2041 gcgggcgcct gccccgctga tgtagggctg agccctaccc ggcgggctcc agattgcggt
     2101 tcacccggaa caggaccgac cctttccgtc tgagaacgct gaccgagcga cggtttgccg
     2161 ccccatccgc agtggtatga cttcccagga ggcccgaaat ggcaacgaaa tcactggaga
     2221 agttgagtaa gaccgaattg tacgagcgtg ctcaggaggc cgatgtcccg ggtcgctccc
     2281 agatggacaa ggatgagttg atcgaagccc tctccgatgg ggggaacggc acgtcgaagg
     2341 gccggagccc aaagtcgaag cgctcgatct ggaacggggc gatcacattc ggtctgataa
     2401 cgatccctgt cgggctctat acggccaccg aggacaaaga catctcgttc cacctgctct
     2461 ccggcaagga cgagtctcgc atccagtaca agcgggtttc gacgaagaca ggccgtgagg
     2521 tcgattggga tgacatcgtc aagggctacg agtacgagaa gggcaagtac gtggtgttca
     2581 cgcccgaaga gctcgagcag atcgctccgg acagcgcacg cagtgtcgat gtcgtgcagt
     2641 tcgtggacgc ctccgaggtc gatccgatct tcttcgagaa gagctactac gtcgtgccga
     2701 ccgacgtggc ggtcaaggcc tatcagctct ttgtccgagc tcttgaagag tctggccgcg
     2761 tcgccgtggc caaggtggcc atccgtgaga aggagcgtct ttgcaccctc cgggtgagag
     2821 atgggtcgct cgtcctcgaa acgatgaaat ggccggatga gatccgggac gccgacttcg
     2881 aacagctcga caagaggacg cgcctctccg ctcaggaact gaagatggcg cgtcagctga
     2941 tcgatcagct gaccgggaaa ttcgatccgg aggtcttcga agacagctat cggcaaaggg
     3001 tcgaggaggc gatcgaggcg aaggtcgagg ggaacgagat cacggtgacg ccggccgcgg
     3061 cgccgaccga gaaggtcgtc gacctgctcg aggcgctcaa atcgagtgtg gagcagacca
     3121 agaccaggcg ctcggcgtga ccctgggcga ccgcccggca gacgacccaa gccggcccat
     3181 cgaggagtgg agcgacgagg agctcgttgc ccaatttcga tccatcaagg gagagctcac
     3241 atccgaagcc ccgggatcct gggatctcga cggcgctccc gccctcgtca tcgaagaaga
     3301 gatgaagcga cggggcctca cccccgatcg ggaggatctg atccccgatg ccgggcctga
     3361 tccggataca ccggtctgat gcccgctgca tctccggggt agcccgggca cggatcgtac
     3421 gattcatcct gtgaccacgg gccccagcat cggcttgacc gcttacgagg agcggagggt
     3481 cgaggagctt cgccggtgga aacgacgcgc caacggcttt ctcctcgggg cgctcgtcgt
     3541 tttcctggtc agtcatttct tcggcggcga gaccgggatc aacggtttca tccgggccgc
     3601 atccgaggcg gccctcatcg gtggcatcgc cgattggttc gcagtcacgg cattgttccg
     3661 ccaccccctc ggcatcccca tcccccacac cgcgctgatc ccccgcagca aggatgagat
     3721 cgggcgtggt ctggccgaat tcgtccagca gaacttcttg gacccggcca acctgcggaa
     3781 ctggctgatg aacgctgaca tcgccggccg cttggcgcaa tggctggcct cctccgacca
     3841 cgccacgctg gcggcacgac ggacaatcga gaccggctcg acgattgctc agtctctcga
     3901 cgatgaacgg gtggcaacgg tcgtcgccca gacctctctc gactggctgc gacagacgcc
     3961 gatcaccccg atcatctccc tggtggtcga tgcctggatg aagggtggtc gcactctgca
     4021 gtcggtggag gccacgctca ggggactcga tgtcgtgatc gtcgacaacc tgccctattt
     4081 cagagacagt ttcgccaaga cgtcaccatg gtggatgccg gcttgggcgg gagagctcgt
     4141 cttcgagcgg atcattgcct ccttccggct actcgtcgac gaggtcgcgt ccgatccgtc
     4201 acatccgatc cgggccgaca tcgagatgat gctctcgcgg atcgccgacg acctgcgcaa
     4261 ctctgcggat ctggccgagc gcatagagga atcgaagctg cacatcctgg actcaccgga
     4321 gttggccgcg gccgtccaga ctcagtggca agttgtccag gagagtctgg gccgggcggc
     4381 gtctcagcca ggttctgatt tggaaaagcg cctatcggag ttgatccagt ggtgggccaa
     4441 ccgcgtgcta gaggacgagg ctctacgtat gcgcatcgac acctggatcg ccgaagccgc
     4501 ggagcggctc gcccatcaat gggatgacga ggtcatcggg atgatc
//
LOCUS       DASTXO010000029         2570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_517130, whole genome shotgun sequence.
ACCESSION   DASTXO010000029 DASTXO010000000
VERSION     DASTXO010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517130"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VFU96_00565"
     CDS             <1..559
                     /locus_tag="VFU96_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEU5111772.1"
                     /translation="PAEDALAAARSCMTALLGPDPLSALEVIGNLRTPDPEQASSLWA
                     LVQSLQLQDPAHRLGVGRWEPFLASVILDSGTRHQGTGAAPGVGAGISTGRYPPDGID
                     VAYRPRSFIIASQPIPNLAPLLWDAAGLVTESGSPAAHLFESARALRVPAVCGVSLPP
                     GDHIVAVDGHTGVVATIPLDGDDHV"
     gene            552..1427
                     /locus_tag="VFU96_00570"
     CDS             552..1427
                     /locus_tag="VFU96_00570"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5111773.1"
                     /translation="MSESIGVVGLGVMGSAMARNLIGAGHQVLGFDIDPGKVSTFADI
                     GGEPARSADEIAGVSEIMIFSLPTTSALASVAAEIARQERPGLVCLETGTFPLPDKVA
                     ARDVLAGSGIDLLDTPLSGTGLQAADATLVVFASGSRQGFERARPVFDAIGRSTHYLG
                     EFGNGSKMKYVANLLVAVHGLAAAEAHALGIAAGLDPARVQEVMEDGVGSSKIFEIRG
                     PMMVADDYPAAARLDILLKDSKLIRDFARSVGAPTPLLDAAVPMYQASSEAGLGDLDA
                     AALCRYLEGLGGLER"
     gene            1424..>2570
                     /locus_tag="VFU96_00575"
     CDS             1424..>2570
                     /locus_tag="VFU96_00575"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein"
                     /protein_id="HEU5111774.1"
                     /translation="MIGSPLRRVEDPRLITGQGRYVDDINMPGMIHAVFVRSVEAHGF
                     IDSVTRDGACQGEMYTASDLGLDRPMPNQYPSPLITQSIQASPLAVDEVCYVGEPVAV
                     VVAETAAEAVDCADAVHVEYSPLPVVIDHRRALAADSAPAHHASTSNLVGTLSAQFGE
                     VDDAFSGADRVVSIEVDQHRGALASMEGRAVLAAWDEVDRRLTVWTSTQAPHAVRAHL
                     ASYLGLSPDEVRVVAPDVGGGFGPKAAVYAEEYVIAALALELRRPVKWTERRREHFVA
                     TNQQRGQSGRVEAAVDASGHILGLRARLVHDCGAYVPYGIVVPMTTLRLLSGPYAIEA
                     LDATVDVVFTNATPTGAIRGAGRPNATFALERLVDAVARELELDRAEV"
BASE COUNT          400 a          882 c          842 g          446 t
ORIGIN      
        1 gcctgccgag gacgcactgg ctgcggctcg gtcctgcatg acggcgcttc tcgggcctga
       61 ccccctgtct gcgctcgagg tcatcggcaa tctccgcacg cccgatccgg agcaggcttc
      121 gtcgctttgg gctctggtgc aaagtctgca actacaggac ccggcccacc gacttggagt
      181 cggcagatgg gagcccttcc tggcctcggt catcctcgac tcgggaacca ggcaccaggg
      241 caccggggcc gctcccggtg tcggggccgg gatctcgacg gggagatacc cccccgatgg
      301 gatcgatgtc gcctaccgcc ctcgatcctt cattatcgcc tcccagccca ttcccaatct
      361 ggctccactc ctctgggacg ctgccgggtt ggtgaccgag tcgggcagcc cggcagccca
      421 cctgttcgag tcagcacgcg ccctcagggt ccccgcagtg tgcggcgtca gccttcctcc
      481 cggggaccac atcgtggctg tcgatggcca caccggcgtc gtcgcgacca tccccctcga
      541 cggagatgac catgtctgag tcgattggag tcgtcgggct cggggtgatg ggctcggcca
      601 tggccagaaa cctgatcggc gccggccatc aggtcctcgg gttcgacatc gaccccggca
      661 aggtctccac attcgccgac atcggaggcg agccggccag gtcggccgat gagattgccg
      721 gcgtctccga aatcatgatc ttctccctcc ccacgacatc cgcactggcc tcagtcgctg
      781 cggagatcgc gcggcaggag cgtcccggtc tggtttgcct cgagactggc acgttcccac
      841 tgcccgacaa ggtcgccgct cgtgacgtcc tggcgggctc cgggatcgat ctcctcgaca
      901 ctccactctc ggggaccggt ctccaggcgg ccgatgcgac actcgtggtg ttcgcctcgg
      961 gtagccgcca gggattcgag cgggcccgac cggtcttcga cgcgatcggc cgctctacgc
     1021 attatctggg ggaattcggc aacggatcga agatgaaata cgtcgccaac ctcctggtcg
     1081 cggtgcatgg actggccgcc gccgaagccc acgctctcgg tattgccgcc gggctcgacc
     1141 cggcccgcgt ccaggaggtg atggaggacg gggttggctc gtcgaagatc ttcgagatcc
     1201 gggggccgat gatggtcgcg gacgactacc cggccgcggc ccgtctcgac atcctcctca
     1261 aggactccaa attgatcagg gacttcgcga gatctgtcgg tgcgccgacc cctctcctcg
     1321 acgccgcggt ccccatgtac caggcttcga gcgaagccgg tcttggcgac ctcgacgcag
     1381 ctgcactatg ccgatacctg gaggggctgg gcgggttgga gcggtgatcg ggtcgcccct
     1441 ccgccgggtg gaggacccga ggctgatcac ggggcagggc cgatatgtcg acgacatcaa
     1501 catgccgggg atgatccatg ccgtcttcgt ccgaagcgtc gaagcacacg gatttatcga
     1561 ctcggtcact cgggacgggg cttgccaggg cgagatgtac acggcctccg acctcggttt
     1621 ggatcgaccg atgcccaatc aatacccgtc gcctctcatc acacaatcga tccaagcctc
     1681 accactcgct gtcgacgagg tgtgttacgt cggcgaaccg gtggcggtcg tcgtggccga
     1741 gacggcggca gaggcggtcg actgcgccga cgccgtgcac gtcgagtaca gccctctgcc
     1801 ggtggtcatc gatcatcgaa gggccctcgc tgccgactca gcgccggcgc accacgcctc
     1861 gacatcgaac ctggtcggca cactttccgc ccaattcggg gaagtcgatg acgcgttcag
     1921 cggtgcagat cgcgtggtgt ccatcgaggt agaccagcat cgcggggccc tggcctcgat
     1981 ggaggggcgg gcggtgctcg ccgcgtggga cgaggtcgat cggcggttga cggtatggac
     2041 ctcgacccag gctcctcacg ccgtccgcgc ccacctggcc tcctacctcg gtctctcccc
     2101 cgacgaggtc agagtcgtgg cccccgacgt cggtggcggc ttcggtccca aggcagccgt
     2161 ctacgccgag gagtacgtca tcgccgccct cgccctcgag ctgagaaggc cggtgaaatg
     2221 gaccgagcgg aggagggagc acttcgtcgc caccaaccag cagcgaggtc agagcggaag
     2281 ggtggaggcg gcggttgacg cgagcggtca catcctgggc ctccgggcgc ggctggtcca
     2341 tgactgcggg gcgtatgtgc cctacgggat cgtggtgccg atgaccaccc tgcgtctcct
     2401 ctctgggccc tatgcaatcg aggcgttgga cgccaccgtc gacgtcgtgt tcaccaacgc
     2461 cacgccaacc ggggcgatca ggggtgcagg ccgccccaac gccaccttcg cgctcgagcg
     2521 tctcgtagac gccgttgccc gcgagctcga actggatcgc gccgaggtgc
//
LOCUS       DASTXO010000030         2258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1106621, whole genome shotgun sequence.
ACCESSION   DASTXO010000030 DASTXO010000000
VERSION     DASTXO010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106621"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VFU96_00580"
     CDS             <1..486
                     /locus_tag="VFU96_00580"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014503633.1"
                     /note="catalyzes the conversion of N-acetylornithine to
                     N-acetylcitrulline in an alternative arginine biosynthesis
                     pathway; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine carbamoyltransferase"
                     /protein_id="HEU5111775.1"
                     /translation="TWSYHPKPLNTAVANSALLIAAKFGMNVTLLCPTEDFLLDDRYM
                     NIATRDAGANGGSVTVTHDIETGYEGVDVVYAKSWGALPFFGRWEEEKPIRDKYQDFI
                     VDSRKMAMTSDAVVSHCLPMRRNVIMTDEVFDSPSCIAYDEAENRLHVQKAIMTALID
                     Q"
     gene            483..1832
                     /locus_tag="VFU96_00585"
     CDS             483..1832
                     /locus_tag="VFU96_00585"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019960029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEU5111776.1"
                     /translation="MSEERVPGSPNRDVRQTIVHLLSNMSDGREIRSYLQRFSEVDRS
                     RFAVIKIGGAILAEHLEETAAALAFLHTVGLTPIVIHGGGTQLDRALDERGLESRRID
                     GLRVTEPDVLDAARDTFIEQNILLVEAIREQGVAAHGLTAGAFEAGYVDREKYGLVGE
                     PTEVHIALIRSIVDSGAIPILTCLGIAPGGQIVNMNADAATRALVHAVQPLKIIFLVD
                     VGGLLDDSGRPIESINLASDYEHLMAQDWVHSGMRVKLAEIKRLLDDTPLSTSVSITT
                     PSGLVKELFTHGGSGTLVRKGELIERVTDPRSIDESQVVKLVEEAFGRPLKEGWWHGL
                     TLDGAYLSESLRAGAIVSRVGDISYLDKFAILEDARGEGLARTVWAQLIADHPVLYWR
                     SRTDNRFNSFYADEADGSVKRGPWTVFWKGETDFDLIAPRVETIAALPPSFAGDDDE"
     gene            1825..>2258
                     /locus_tag="VFU96_00590"
     CDS             1825..>2258
                     /locus_tag="VFU96_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011642306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEU5111777.1"
                     /translation="MSERYAVGVVGARGHTGAELVRLIRDHPSLQLVFAGSRELAGKP
                     VPGLDDVVFDSIRPHDLSGQGLDVVILALPDGTGDAYVDALDEETVVVDISADHRFDD
                     AWAYGLPELFRDKLQGARRISNPGCYATAMQIALAPLVADVSG"
BASE COUNT          406 a          747 c          716 g          389 t
ORIGIN      
        1 acctggagct atcaccccaa gccgctgaac acggcggtgg ccaattcggc actgctgatc
       61 gcagccaagt tcgggatgaa cgtcaccctg ctctgcccga cggaggattt cctgctcgac
      121 gatcggtaca tgaacatcgc aacccgcgac gccggtgcca atgggggcag cgtcaccgtg
      181 acccacgaca tcgagaccgg ctacgaggga gtcgatgtcg tctacgccaa gagttgggga
      241 gcgctccctt tcttcgggcg ttgggaggag gagaagccga tccgggacaa gtaccaggac
      301 ttcatcgtcg actcccgaaa gatggcgatg accagcgacg cagtcgtcag ccactgtctc
      361 ccgatgagac gcaacgtcat catgaccgac gaggtcttcg acagcccgag ttgcatcgcc
      421 tacgacgagg cggaaaaccg tctccacgtg cagaaggcga tcatgaccgc tctcatcgac
      481 caatgagcga ggaacgggta ccgggctcac ccaaccggga tgtgcgccag accatcgtcc
      541 atctgctctc caacatgagc gatgggaggg agatccgctc ctacttgcaa cggttctcgg
      601 aggtggatcg ctcgcgtttc gcggtcatca agatcggtgg cgccatcctg gccgagcatc
      661 tcgaggagac ggccgctgcc ctggccttcc tccacaccgt cggcctcacc ccgatcgtca
      721 tccacggggg cggcacccaa ctcgaccgtg ccctcgacga gcgcgggctc gagagccgaa
      781 ggatcgatgg gctcagagtc accgagcccg atgtgctcga cgcggcccgc gacaccttca
      841 tcgagcagaa catccttctc gtcgaagcca tcagggagca gggcgtcgcc gcccacggcc
      901 tcaccgcggg cgccttcgag gcgggatacg tcgaccggga gaagtacggg ttggtgggcg
      961 agccgaccga agttcacatc gccctgatcc gctccatcgt cgactccggg gccatcccca
     1021 tcctcacctg tctcggcatc gccccgggtg ggcagatcgt gaacatgaac gccgacgcgg
     1081 cgacgagggc cctggtccac gccgtgcagc cactgaagat catcttcctg gtcgatgtgg
     1141 gcggcctgct ggacgacagc ggacggccga tcgagtcgat caatctcgct tccgactacg
     1201 agcacctcat ggcccaggac tgggtccact cggggatgcg ggtcaagctg gccgagatca
     1261 aacggctcct cgacgacaca cccctctcca cctcggtctc gatcaccaca ccaagtggcc
     1321 tggtcaagga gctgttcacg cacggcgggt cgggcaccct cgtgcgcaag ggggagttga
     1381 tcgaacgcgt caccgatccc cgctcgatcg acgagagcca ggtggtcaag ctggtcgagg
     1441 aggccttcgg gaggcctctc aaggagggct ggtggcacgg actgaccctc gacggcgcct
     1501 atctgtccga gagcctgcgg gccggggcaa tcgtgtcacg tgtcggcgac atctcctatc
     1561 tggacaagtt cgcgatcctc gaagatgctc gtggtgaggg cttggcgagg acggtgtggg
     1621 cgcagctgat agccgatcat ccggtgctct actggcggtc ccgcaccgac aatcggttca
     1681 actccttcta cgcagacgag gccgacggct ccgtgaagcg agggccctgg accgtgttct
     1741 ggaagggcga gaccgacttc gacctaatcg ccccccgggt cgagacgatc gcagcgcttc
     1801 ccccttcgtt cgcgggcgac gacgatgagt gagcgctacg cggtcggagt cgtgggggcg
     1861 cgcggccaca ccggtgccga gttggttcgc ctgatccgtg atcacccctc gctccagctg
     1921 gtcttcgcgg gctctcgtga gctcgccggc aagcccgtgc cgggcctcga tgatgtcgtg
     1981 ttcgattcga tccgccccca cgatttgagc ggacagggtc tggatgtggt gatcctggcc
     2041 ctacccgatg ggaccggcga tgcatacgtc gatgccctcg acgaggagac cgtcgtcgtc
     2101 gacatctccg cggatcaccg gttcgacgac gcttgggcct acgggcttcc cgagctgttc
     2161 cgggacaagc tgcagggtgc gcggcgaatc tccaaccccg gttgctacgc cacagccatg
     2221 cagatcgccc tcgctccgct cgtcgccgac gtatctgg
//
LOCUS       DASTXO010000031         5269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1696358, whole genome shotgun sequence.
ACCESSION   DASTXO010000031 DASTXO010000000
VERSION     DASTXO010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1696358"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VFU96_00595"
     CDS             complement(<1..627)
                     /locus_tag="VFU96_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111778.1"
                     /translation="MSLAVFPFKTEQPSLVASNLATAAAHPAVDEVWAVAPREGEEEV
                     AVRAMAREVSRARAKPIKVIVQERLGRYRSGKGDAMNTALALAAGQGRDRVHFYDADI
                     TNFDASWIEGAEAAADRGFGVVRHRFPRAATDAMITWMITRPALARLFPGTLLPRLDQ
                     PLGGEVLLTGRAVEALAGSDLVRERSDWGIDTVLTYATSIMDLGVYEHH"
     gene            683..2356
                     /locus_tag="VFU96_00600"
     CDS             683..2356
                     /locus_tag="VFU96_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018352939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5111779.1"
                     /translation="MEEMVGRVLGTVDATPLEFWVGVSEGEYLELDDVVALERVLPTG
                     ELVKIFGTISQVRARHEGARFDSDVFLIEDGVLPAEVMEAAQVTATRVEPEIFVPPRP
                     GQPVRRAHGTERDQALFFDRMERRFPVGASRNGEPLFANFEFINGQRGAHVNISGVSG
                     VATKTSYATFLLHSLFTSGVLGSAVVNTRALIFNVKGEDLLHLDRPNTSLDEDAERLY
                     RALGLPAEPFGSVGLFAPPRPGSDRAVADVSSRSDLTSFFWTLAEFCHEDLMVFLFAD
                     AEDERAQYTMVVFNVMAQLRHADAGDGGSVRIEGETVRTFRDLVDLVTRKVQEEESRN
                     EWAGPHIGAGTVNAFVRRLQGAVRHVERLIRGDIANPDRHRIEVQGHQVTVVDLHNLN
                     DRAKRFVVGVAVRRAFEDKERAGQSDELLFLVLDELNKYAPREGTSPIKEVLLDVAER
                     GRSLGVILIGAQQTASEIERRIIANSSIRVVGRLDSAEASREEYGFLPAAQRQRATIL
                     KPGTMLLAQPEIPVPLVLEFPFPAWATRASEAGVAPEGLDGPADPFEDL"
     gene            2422..3552
                     /locus_tag="VFU96_00605"
     CDS             2422..3552
                     /locus_tag="VFU96_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEU5111780.1"
                     /translation="MRLLHTSDWHVGRKIRGRSRADEHRAVLAEIVGIAQDRRVDITL
                     VAGDLFDISSPSPEDESIVYRALLDLAEIGPVLVVGGNHDSAARLEAVKPLLDLGRIT
                     VVARPTRPADGGVAVFDSFGLKVACLPFVSQRGIVKAAEIMDLDADQHAQSFEARLRR
                     IIAGLTDDMGTDTVNVVTAHLTVYGAESGGGEREAHIFGYAIPPQAFPGSLSYVALGH
                     LHRQQRIPASAPVWYSGSPMQLDFGEVSDVKGVLIVEAEAGKPASVAQVAVESGTKLV
                     QVRGTLEQVLARAGDLEGCYVKVLLDEPARAGLNEEVREAIPGAVDVIITQTEDRSPR
                     DRVERVGRSPAALLEAYLASKGVDDPAVIELFRELEQEVVSR"
     gene            3549..>5269
                     /locus_tag="VFU96_00610"
     CDS             3549..>5269
                     /locus_tag="VFU96_00610"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEU5111781.1"
                     /translation="MRPQLLIMEGFLAYRRRTEIDFTDADLFVLVGPTGSGKSSVIDG
                     MTFALYGSIPRVGKGSVAPLISAQADSGRVEFRFSVGEESYSAVRLMMRQGSGATTTE
                     ARLLRGESQEVLAGTADEVTAAVTDLLGLRYEHFIKAVVLPQGAFADFLTDQPRDRQA
                     LLGALLDMGLYEQVMQLANQRAKLAEARAKTVGDSLAKLDVPSPEKVDEATRALAELA
                     GARMELPARVEATTRLDDVLAEARLKHAASVESLTRLRAIEVPDDLETLDQDRASALM
                     HLESVESSLAEAIEAVERIDEAIAGHPPPASLESTRADHLLHRELAGRRSQLALESLA
                     AAADEIAAQRDEMRRSVEKLRIEHAAHDLRRELTAGDTCPVCQSVVATVAEDADGPNV
                     SLDQLATGLRELEERTDRARDGFKEAEGQAKQIDRQLEELRGRLERAGDLEAIEQAIT
                     MVKGLVESKAALERAVTEAQLNVEQARLVVADLNDRSSGLLDALLAARDRIASEQPPL
                     PGNDAIDAWRRFVIWLGETTATRSQELAALDTAVDVAAEGSTTVEAELRSWLEHFGIA
                     SIESPDR"
BASE COUNT          911 a         1729 c         1731 g          898 t
ORIGIN      
        1 gtgatgctcg tatacgccga ggtccatgat cgaggtggca taggtcagca cggtgtcgat
       61 tccccagtcg gagcgctctc gcaccaggtc tgacccagcc agggcctcga cggcccgtcc
      121 ggtgagcagg acctcaccgc cgagcggctg gtcgagtctc gggaggagcg tcccggggaa
      181 gaggcgggcc agggcaggcc gcgtgatcat ccaggtgatc atggcgtcgg tggcggccct
      241 cgggaaccgg tgtctcacca ccccgaaacc gcgatcggcc gcggcttcgg ccccctcgat
      301 ccacgacgcg tcgaaattgg tgatgtcggc gtcgtagaag tggacccgat cgcggccctg
      361 cccggcggcg agagccaaag ccgtgttcat cgcgtctccc ttccccgatc ggtaccgacc
      421 cagccgctcc tgcacgatga ccttgatcgg cttggcgcgg gcgcgggaca cctccctcgc
      481 cattgcccga actgccacct cctcttcgcc ttccctgggg gccaccgccc acacctcgtc
      541 gacggctggg tgtgcggctg ccgtagccag gttcgaggcg accagcgaag gctgctcggt
      601 cttgaacggg aacaccgcaa ggctcacctg gcgagcctag gtagacctca acctgtccca
      661 cgccgggtct actctcggcc ggatggagga gatggtcggt cgtgtgctgg ggaccgtcga
      721 cgccaccccc ctcgagttct gggttggcgt gagcgaaggc gagtacctgg agttggacga
      781 tgtcgtcgcc ctcgaacggg tcctacccac cggtgagctc gtcaagatct tcgggacgat
      841 cagccaggtg cgcgcccggc acgaaggggc gcgcttcgac tccgatgtgt tcctcatcga
      901 ggacggcgtg ctgccggccg aggtcatgga ggcagcccag gtcaccgcca cccgcgtcga
      961 gcccgagatc ttcgtcccgc cccgtcccgg acaacccgtg cgccgggccc atggcaccga
     1021 gcgcgatcag gcgctgttct tcgatcggat ggagcgcagg ttcccggtcg gggcctcgcg
     1081 caatggggag cccctattcg ccaatttcga gttcatcaac ggccagcgcg gcgcccacgt
     1141 caacatctcc ggtgtctccg gcgtcgccac caagaccagc tacgcgacct ttctcctgca
     1201 ctcgctgttc acgtccgggg tgctgggctc ggcggtggtc aacaccaggg ccctgatctt
     1261 caacgtgaaa ggtgaggacc tgctccacct cgatcgaccc aataccagtc tcgacgagga
     1321 tgccgagcgt ctctatcggg ctttgggcct gcccgccgag ccattcgggt cggtcggtct
     1381 gttcgccccg ccacgacccg ggtctgaccg ggcggtggca gacgtgagca gccgctccga
     1441 cctcacttcg ttcttttgga ccctggccga gttctgccac gaggacttga tggtcttcct
     1501 cttcgccgat gcggaggacg agcgtgccca atacacgatg gtcgtcttca acgtcatggc
     1561 ccagctcaga cacgccgacg ccggcgacgg aggatctgtc cgcatcgaag gcgagacagt
     1621 caggacgttc cgggacctag tcgatctggt gacgcggaag gtccaggaag aggaatcacg
     1681 gaacgagtgg gccgggcccc atatcggggc gggcacggtc aacgctttcg tgcggcgact
     1741 tcagggtgcg gtccgccatg tcgagcgcct gatccgcggt gacatcgcca atcccgatcg
     1801 gcaccgaatc gaggttcagg gccatcaggt caccgtcgtc gacctccaca atctcaacga
     1861 tcgggcgaag cggttcgtgg tcggtgtggc ggtccgacgc gcgttcgagg acaaggagag
     1921 ggctggtcag tccgacgaac tgttgtttct cgttctcgac gagttgaaca aatacgcccc
     1981 gagagagggc accagcccga tcaaggaggt cctcctcgat gtcgccgaac gtgggcgttc
     2041 tctcggtgtg atcctgatcg gggcccagca gactgcgtcg gagatcgaga ggcggatcat
     2101 cgccaactcc tcgatccgcg tggtcggccg gctcgattcg gccgaggcgt cgcgagagga
     2161 gtacggattc ctccccgccg cgcaacgaca gcgcgccacc atcctcaaac cggggacgat
     2221 gctgctggct caaccagaga taccagtccc gctggtgctg gagttcccct tcccggcctg
     2281 ggcaactcgg gcctccgagg caggcgtggc accagaaggt ctcgacggcc cggccgatcc
     2341 attcgaggac ctctaggagc ctcccaggat ctgtccccgc cccgacctac gatcgatgga
     2401 gatgacctgg ccgaacccgc cgtgagactc ctccacactt cggattggca tgtggggagg
     2461 aagattcgtg gccgctctcg ggccgacgag caccgggccg tgcttgccga gatcgtgggc
     2521 attgcccagg atcgtcgtgt cgacataacg ctggtcgcag gcgacctctt cgacatctcc
     2581 tcgccatcac cggaggacga atcgatcgtc taccgggcgt tgctcgacct ggcagagatc
     2641 ggcccggtcc tcgtggtggg aggcaatcac gacagcgcgg cacgtctgga ggcggtgaaa
     2701 ccgctcctcg acctgggccg gatcactgtg gtggcccgtc cgacccggcc cgccgacggc
     2761 ggtgtggccg tgttcgactc atttggtctc aaggttgcct gcctcccctt cgtgtcgcag
     2821 aggggcatcg tcaaggcggc ggagatcatg gatctcgacg ccgatcaaca cgcccagagc
     2881 ttcgaggccc ggctgaggcg gatcatcgcc ggtctcaccg atgacatggg gaccgacacc
     2941 gtcaatgtgg tgaccgcgca tctgacggtc tacggggcgg agagcggtgg cggcgagcgg
     3001 gaagcccaca tttttggcta cgccattcct cctcaagcct tcccgggcag cctctcctac
     3061 gtggcgctcg ggcatcttca tcgtcagcag cggattccgg cctcggcccc ggtctggtac
     3121 agcggatctc cgatgcagct cgacttcggc gaggtctccg atgtcaaagg ggtcctcatc
     3181 gtcgaggccg aggcggggaa gccggcatcg gtggcccagg tcgctgtgga aagcgggacc
     3241 aagctcgtcc aggtgcgggg caccctggag caggtccttg cccgcgccgg ggatctggaa
     3301 gggtgctacg tgaaggtgct gctcgacgag cccgccaggg caggcctcaa cgaggaagtg
     3361 cgagaggcga tcccgggggc ggtagacgtg atcatcactc aaaccgaaga ccgatcacct
     3421 agagaccggg tcgaacgtgt cggccgttca ccggctgccc tcctggaggc ctacctcgca
     3481 tcgaagggcg tcgacgaccc cgccgtgatc gagttgttca gggagctcga gcaggaagtg
     3541 gtctcgaggt gagaccacag cttctgatca tggagggttt cctggcctac cgccgccgga
     3601 ccgagatcga cttcaccgac gccgatctct tcgtgctcgt cggtccgacc ggatcgggga
     3661 aatcgagcgt gatcgacggc atgaccttcg ccctctacgg gtccatccca cgcgtcggta
     3721 agggctccgt agcaccgctc atctcggccc aggccgacag tggtcgagtg gagttccggt
     3781 tctcggttgg cgaggagagc tacagcgctg tccgtctcat gatgcgacag ggctcggggg
     3841 ccacgaccac cgaggcccgt ctgctccgcg gtgagagtca agaggtgctg gcggggaccg
     3901 ccgacgaggt gaccgctgct gtcaccgacc tcctcggact tcgctacgaa catttcatca
     3961 aggcagtcgt cctgccccaa ggggccttcg ccgactttct caccgaccag ccaagagacc
     4021 gtcaggccct tctcggagcg ttgctcgaca tgggtctcta cgagcaggtc atgcagctgg
     4081 ccaatcagcg ggccaaactc gccgaggcca gggcgaagac ggttggagac agcctcgcca
     4141 agctcgacgt cccctctccc gaaaaggtcg atgaagcgac tcgagccctc gcggagctgg
     4201 ccggagcgag aatggagctg ccagcccgtg tcgaggcgac gacccggctc gacgatgtgc
     4261 tcgccgaggc gaggttgaag catgccgcct ccgtggagag tctcacccgc ctccgggcaa
     4321 tcgaagtgcc cgatgacctc gaaaccctcg accaggaccg ggcgtccgcc ctcatgcacc
     4381 tcgaatcagt cgagtcctcc ttggccgagg ccatcgaggc ggtcgagcgc atcgacgagg
     4441 cgatcgcggg ccacccgccg ccagccagtc tggaatcgac gagggccgac catctcttgc
     4501 atcgcgagct cgccggccgg agatcgcaac tcgctctcga atcgcttgca gctgccgccg
     4561 acgagatcgc cgcccaacgc gacgagatgc gccgttctgt cgagaagctc cgcatcgagc
     4621 atgcagctca cgacctgcgc cgagagctca ctgccggcga cacatgcccg gtctgccaat
     4681 cggtagtggc cacggttgca gaggacgccg acgggccgaa cgtgtcactc gatcagctcg
     4741 ccacagggct gcgtgagctc gaggagcgga cggatcgtgc tcgggacggt ttcaaggaag
     4801 ccgagggcca ggcaaaacag atcgatcgtc agctcgagga attgagaggg cgtctcgaga
     4861 gggcgggcga cctcgaggca atcgagcagg cgataaccat ggtcaagggg ttggtcgagt
     4921 ccaaagctgc cttggagcgg gcagtgactg aggcgcagct gaacgtcgaa caggctcgtc
     4981 tcgtcgtcgc ggatctcaat gacaggagct ccggtctgct cgatgcgctt ctcgccgcca
     5041 gagaccgaat cgcatccgaa cagccacctc ttcctggcaa cgacgcgatc gacgcctggc
     5101 gtcggttcgt gatctggctg ggggagacga ccgccacccg atcacaggag ttggccgcgc
     5161 tcgacacggc cgtcgatgtg gcggccgaag gctcgacgac ggtggaggcg gaacttcgct
     5221 cctggctgga acatttcggc atcgcctcga tcgagtctcc tgaccgtga
//
LOCUS       DASTXO010000032         3204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_259515, whole genome shotgun sequence.
ACCESSION   DASTXO010000032 DASTXO010000000
VERSION     DASTXO010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259515"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="VFU96_00615"
     CDS             <1..1099
                     /locus_tag="VFU96_00615"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEU5111782.1"
                     /translation="AVVEGAALGGYQFRAYKTEGEGLSIEILELIEGNEDELGEALIT
                     TEATNLARNWVNTPALDKAPEILAEEMREAAEAAGLTAEVWDEDRIVAEKLGALLGVA
                     AGSDRPPRLVVIEYQPESAGAHLGLVGKGITFDSGGLSIKTAQYMEEMKDDMSGAAAV
                     VAATTAIARLGLPVRVTTVVPLTDNAVGGKATRPGDVLRPVAGPTIEVLNTDAEGRLI
                     LADGLGVVRRYEPDLIIDVATLTGAAHVALGDKIAAVFGSDSDVAAQVLAAASRAGEH
                     FWEMPLFKEYKKSIESDIADLKNISGGRYGGAITAALFLSSYVDDARWAHLDIAGPAR
                     SRETAGENVKGASGVGVRTLVELARGMAEPA"
     gene            1129..2409
                     /locus_tag="VFU96_00620"
     CDS             1129..2409
                     /locus_tag="VFU96_00620"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU5111783.1"
                     /translation="MRRRFLIRSSLSDLASLGVAMVVASLYVFGTFLPWNSSQGAEVI
                     PMVLYLTLSLILFSFLTAQMSGPGVPRPTYGRMFAIFLGTGGLTALLLLFSRQYFSRS
                     FLLITAVTWLGLATAHRIVRRRRPWTERIAVITGEKQLAEDLIDSPHAEIVWVLEPKS
                     ESVPELPDRDVTMAVDLKVVLSERVAQFLSSCDVAGYTIRPFTSTYEEHTGRVPLVHL
                     AEGWEISAPLLEVAPWLPGKRAIETLATLITSPIWVTLSLVVATYLRLTSSGPVLFRQ
                     ERVGLGGAPFVMYKFRTMGHDAEKDGPSFASVRDHRIIPGGRFLRKSRLDEIPQLLNV
                     LRGEMSLVGPRAEQVPFADVFRKQIPFYAHRHLVRPGITGWAQVNYGYADDQADTIEK
                     LTYDLYYIKHMSPVMDLRVLWRSVWTVLTGFGAR"
     gene            2469..>3204
                     /locus_tag="VFU96_00625"
     CDS             2469..>3204
                     /locus_tag="VFU96_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111784.1"
                     /translation="MAGGLIGAIGAYIFQAYGGRQLGTEAFAPVAQLWTVFFIIATVL
                     LVPVEQYITREVAGGRKVIPVDLVPAMTVTAIGAVIGGGFVLFTMDRLFAGSAQFVIQ
                     IVLLMIGYGLLVVGKGVYAGSRQYAKVGWVLIVESAVRLAAGIVAIQLAASAESLGWA
                     MVLGGFAVLGMGWWRYDQGHTRPPAAPPTRFLGGYVGGTAASQVLLGAAPVAVALLGG
                     DEAIQSIVFATFTLFRAPLTLIFSLQG"
BASE COUNT          531 a          997 c         1046 g          630 t
ORIGIN      
        1 ggcggtagtc gagggtgccg cgctgggtgg ctaccagttc cgcgcttaca agaccgaggg
       61 tgaggggctg tcgatcgaga ttctcgaact gatcgaaggc aacgaggacg agctgggtga
      121 ggcgctgatc acaacggagg ccaccaacct ggcccggaac tgggtcaaca cacctgcact
      181 ggacaaggca cccgagatcc ttgccgagga gatgcgggag gcggccgaag ctgcaggcct
      241 cacggccgag gtatgggacg aggatcggat cgtcgccgag aaactgggcg ctctccttgg
      301 cgtggccgcc gggtctgatc ggcccccccg cctcgtcgtc atcgagtacc aacccgaatc
      361 ggccggtgcc catctcggcc tcgtcggcaa gggaatcacc ttcgactccg gcggtctttc
      421 gatcaagacc gcccaataca tggaggagat gaaggatgac atgtccggcg ccgccgctgt
      481 cgtggcggcg acgacggcga tcgctcggct cgggctcccg gtgcgggtga ccacggtcgt
      541 gccgctgacc gacaacgccg tcggtgggaa agcgacaaga cccggcgatg tcctcagacc
      601 cgtcgcgggg cccacgatcg aagtgctcaa caccgacgca gagggacgtc tcatccttgc
      661 cgacgggctg ggagtggtcc gccgctacga gccggacttg atcatcgatg tggccaccct
      721 gacgggcgcc gcccacgtcg cccttggtga caagatcgcc gccgtcttcg gatcggattc
      781 ggatgtggct gctcaggtgc tcgcggcggc gtctcgtgcc ggggagcact tctgggagat
      841 gcccttgttc aaggagtaca agaagtcgat cgagtcggac atcgccgact tgaagaacat
      901 ctcaggtggg cggtatggcg gtgcaataac cgctgcgctc tttctctcga gctatgtcga
      961 cgatgcgaga tgggcgcatc tcgacatcgc cgggccggcc cgatcgaggg agactgcggg
     1021 cgagaacgtc aagggagcct caggagtcgg cgtccgaact ctcgtcgaat tggcccgggg
     1081 catggcggag cccgcctgaa gtggctgtca cttcctagca tctcctctgt gcgcagacgt
     1141 tttctgatcc gctccagtct cagcgatctc gcatcgctgg gagtcgccat ggtcgtggcg
     1201 tccctctacg tctttggcac attccttccc tggaactcga gtcagggggc cgaggtgata
     1261 ccgatggtcc tctacctgac gctctccctc atcctgttct cgttcctcac ggcccagatg
     1321 tcggggcccg gagtccctcg ccccacctat gggcgaatgt tcgccatctt cctaggcacc
     1381 ggtgggctga ccgcattgct cctgctcttc agccggcagt acttctcgag atcgttcctg
     1441 ctcatcaccg cggtgacatg gttgggtctg gccacggccc atcgcatcgt gaggcgccgt
     1501 cgaccatgga ccgagcgaat cgccgtgatc acaggtgaga agcagctggc cgaggatctg
     1561 atcgattcgc cccatgccga gatcgtctgg gtgctggagc ccaagtcgga gagtgttccc
     1621 gaactgccgg accgggatgt gaccatggcc gtcgatctca aggtggtcct gtccgagcgc
     1681 gtagcccagt tcctttcgtc gtgtgacgtg gcgggctaca ccatccggcc cttcacgtcc
     1741 acgtacgaag aacacaccgg aagggtcccc ttggtgcatc tggccgaggg atgggagatc
     1801 tcggctccac tgctcgaggt cgcaccgtgg ctccccggca agcgggcgat agagacgctg
     1861 gcgacactga tcacgtctcc aatctgggtc actctcagtc tcgtggtcgc cacgtatctc
     1921 cgtctcacct catcgggtcc ggtcctgttc cgacaggaga gggtgggatt gggcggcgcc
     1981 ccgttcgtca tgtacaagtt ccggacgatg gggcatgacg cggaaaaaga tggccccagt
     2041 tttgccagtg tgcgggacca ccggatcatc cccggtggca ggtttctcag gaaatctcga
     2101 ctcgatgaga taccacagct cttgaatgtg cttcgcgggg agatgagcct ggtcgggcct
     2161 cgcgccgagc aggtcccgtt tgccgatgtc tttcgcaaac agattccctt ctacgcccat
     2221 cgtcatctgg ttcgccccgg aatcacggga tgggctcagg tcaattacgg gtatgccgat
     2281 gatcaagcgg acaccatcga gaagttgacc tatgacctct actacatcaa gcacatgtca
     2341 ccggtgatgg acctgcgcgt gctctggagg agcgtctgga cggtcctcac cgggttcggc
     2401 gcccgttgag cgcaccggtg atagaggccc aaccgagagg cttccgagct cccggtacca
     2461 ccttcatggt ggccggagga ttgatcgggg cgatcggcgc ctacatcttc caggcttacg
     2521 gcggccgtca gctgggcacc gaggccttcg cgccggttgc ccagttgtgg acggtgttct
     2581 tcatcatcgc caccgtgttg ctggtccccg tggagcagta catcacgcgc gaagtggccg
     2641 ggggccgaaa agtgatcccg gtcgacctgg tgccggcgat gacggtgacg gcgatcgggg
     2701 cagtcatcgg gggcgggttc gtgctgttca ccatggaccg gctgtttgcg ggaagcgcgc
     2761 aattcgtgat tcagatcgtg ctcctcatga tcggctacgg gttgcttgtg gtgggcaagg
     2821 gggtctacgc cggcagccga cagtacgcca aggtgggatg ggtcctgatc gtcgagagcg
     2881 cagtacggct ggccgcgggc atcgtcgcga tccaactcgc tgcgtcggcg gagtccttgg
     2941 gctgggcgat ggtgctcggt ggattcgcag tcctcgggat gggttggtgg cgctacgacc
     3001 agggccacac ccgtccccct gccgcccctc ccactcgctt ccttggtggc tatgtcggcg
     3061 gcaccgccgc gtctcaggtc ctcctggggg cagccccggt cgccgtcgcc ctgttggggg
     3121 gtgacgaggc aatccaatcc atagtcttcg ccacgttcac tctgttccga gccccactca
     3181 ccctgatctt ctcgctccag ggca
//
LOCUS       DASTXO010000033         2050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1033267, whole genome shotgun sequence.
ACCESSION   DASTXO010000033 DASTXO010000000
VERSION     DASTXO010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033267"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VFU96_00630"
     CDS             complement(<1..387)
                     /locus_tag="VFU96_00630"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5111785.1"
                     /translation="MPDTSPTDGCLEHSYIDGGDVSKESSRRQRFDSLFEAHSLDVAS
                     YCNWRTSSRADAEDAVAEVFLVAWRKIDLVPPGDGARAWLYATARRVTANTRRSLRRR
                     EALADRISQESRIDDLRSEAVSAEEAV"
     gene            complement(445..1404)
                     /locus_tag="VFU96_00635"
     CDS             complement(445..1404)
                     /locus_tag="VFU96_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015180937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU5111786.1"
                     /translation="MDHPTTLGRSTLTVPRLGVGAMTWGEPRGLARFHPATMAYGGAH
                     GYDEEKGALEASLKAGVNLVDTAAFYSAGASETRVGELARGTEVLIASKFPSGLRFQA
                     DDFPRELAASLKRLGRSNLDLYQHHFPGRVSIPDLMNHMADAVDAGKVTAVGVSNYSA
                     EQMRQAHAALAARGVPLAANQVEYSLLHRQPETNGVLDACRELGISLIAYTPLAGGML
                     TGKYSAENRPTGFYRRVLPRYRRKSLDAIQPVVGLLREIGDRYSRTPSQVALRWLIEN
                     PIVLPIPGAKNGMQAANNAAALSFSLTAEEVAALSDATRAWRA"
     gene            complement(1475..>2050)
                     /locus_tag="VFU96_00640"
     CDS             complement(1475..>2050)
                     /locus_tag="VFU96_00640"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5111787.1"
                     /translation="FGQIDVFIGNAGIFDFGASFVDTPIDRLEEGFDELFALNVKGYL
                     LGVKAAVNALIESSGSVLLTASMSSWHAGVGGVVYTASKHAVVGLVRQLAHELAPKVR
                     VNGVAPGFMRTDIRGPKALGLGDVTPSSLPDLEQIAVATTPLRFLPRPEDYTGHFVQL
                     ASRSNAPATTGIVVACDGGLDVRGIGLPPPG"
BASE COUNT          357 a          652 c          660 g          381 t
ORIGIN      
        1 cacggcttcc tctgctgata cggcctccga tcgcagatca tcgatgcggc tctcctgtga
       61 gatgcgatcc gccagcgctt cccgacgacg cagcgaccgt ctcgtgttgg cggtgactct
      121 gcgggcggtg gcgtacaacc acgcccgagc gccgtcgccc ggtgggacca ggtcgatctt
      181 ccgccaggcc acgaggaaca cttctgcaac ggcgtcttcg gcgtcagccc tcgacgaggt
      241 tcgccagttg caatacgagg cgacatcgag actgtgggcc tcgaataagg aatcgaaccg
      301 ttgccttcgg ctcgattcct tgctcacgtc gcccccatcg atgtagctgt gctccaagca
      361 tccatctgtc ggtgaagtgt ccggcatcca tccgttcgtt tgacggagca tggcttgatc
      421 gagcctggat cgcaggcccg gggctcaagc cctccaggct cgagtcgcat cgctcaaggc
      481 ggctacttct tcggctgtga gtgagaagct gagagctgct gcattgtttg ctgcctgcat
      541 gccgttcttg gcgccaggga tcggcagcac gatcgggttc tcgatcagcc acctcaaggc
      601 cacctgactc ggggtcctcg agtatcgatc gccgatctcg cgcaggagcc cgaccacggg
      661 ttgtatggcg tctagagact tgcggcgata ccggggaagg acacgtcggt agaacccggt
      721 gggacgattc tcggccgagt acttcccggt cagcatgccc ccggcgagcg gggtgtatgc
      781 gatgagactg attccgagct cacggcaggc atcgaggacg ccgttggtct ccggttgtcg
      841 gtgcaggagt gagtactcga cctgattggc tgcaaggggc acgccccggg ccgcaagagc
      901 ggcgtgggct tggcgcatct gttccgctga gtagttgctg acgcctaccg cggtcacctt
      961 cccggcatcg acggcgtcgg ccatgtggtt catcaagtcc ggtatcgaaa ctcgtcccgg
     1021 gaaatgatgc tggtagaggt cgaggttgga gcgtcccagt cttttcaggc tcgcagccaa
     1081 ctcgcgcggg aagtcgtcgg cctgaaatcg gagcccggag gggaacttgc tggcgatcaa
     1141 gacctctgtg ccccgggcca gctcgccgac gcgcgtttcc gatgctcccg cgctgtagaa
     1201 ggcggcggtg tcgaccaggt tgacacccgc cttcagactc gcctcgaggg cgcccttctc
     1261 ctcgtcgtac ccgtgagctc caccgtacgc catcgtcgcc gggtggaatc gtgcgagccc
     1321 ccgaggctcc ccccaggtca tcgccccgac accgaggcgc ggaaccgtca gggtgctgcg
     1381 acccagggtc gtcggatgat ccatctcgtc actccgtcac gcggggggaa tgcccgaagc
     1441 gtaggtccta tcacctagcc gcgatcgggg ggtatcaacc gggaggcggc agtccgattc
     1501 cccgcacgtc gagcccaccg tcgcatgcga caacgatccc agtggttgcc ggggcgttgc
     1561 tgcgtgaggc gagctgaacg aaatgaccgg tgtagtcctc gggccggggc aggaatcgga
     1621 gcggtgtggt ggcgacggcg atctgctcca gatcgggaag cgaggaggga gtgacatcgc
     1681 cgaggcccag cgccttgggg ccacggatgt cggttcgcat gaagccaggc gccacaccgt
     1741 tgacccgcac cttgggcgcc agctcgtggg ccaattggcg gaccaacccg acaaccgcat
     1801 gcttcgaagc cgtgtagacc acacccccca ctcccgcgtg ccaagaggac atggacgctg
     1861 tgagcagaac cgagccacta ctttcgatca gtgcgtttac tgcggctttg actccgagca
     1921 ggtacccctt gacattgagg gcgaacaact cgtcgaagcc ctcttccagt cgatcgatcg
     1981 gcgtgtcgac gaagctggca ccaaagtcga agatcccggc gttcccgatg aacacgtcga
     2041 tctggccgaa
//
LOCUS       DASTXO010000034         2564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_444031, whole genome shotgun sequence.
ACCESSION   DASTXO010000034 DASTXO010000000
VERSION     DASTXO010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444031"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VFU96_00645"
     CDS             complement(<1..293)
                     /locus_tag="VFU96_00645"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5111788.1"
                     /translation="MNQQDFTSEQELFRATVREFCEKEISREYVRECDRERRPPRDVY
                     DKLAAQGWLGINTPVEHGGAGAGAVEVAILLEELGRSFLDLSFWVFRTVTWGGL"
     gene            368..1333
                     /locus_tag="VFU96_00650"
     CDS             368..1333
                     /locus_tag="VFU96_00650"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005256390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEU5111789.1"
                     /translation="MTASIETVRDLAARHSNWGRWGPDDERGTLNLVTPEMVMAAAGL
                     VRAGKTISMALPYDEDGPQTGNLGRFNPIHLMIRDGADALAGTSTRDFYGGVDKHFRG
                     TDDIIIMPLQSGTQWDAFGHVVFEGTIYNGYGAEWVSSKGALKGDVANASQGMVGRGV
                     LLDLPRALGVDWLEPGHAIGSAELERAEEHARVTVGTGDFVFVRTGAMAASRAAGAWG
                     EYAGGEAPGLGLDSVGWVADRDIAAIASDTWGLEVRPNETPDVAQPLHIVFIVHMGLW
                     LGEIFDLDPLAEDCAADGAYEFMFCGPPLPFTRAVGSPLNPMAIK"
     gene            1341..2222
                     /locus_tag="VFU96_00655"
     CDS             1341..2222
                     /locus_tag="VFU96_00655"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEU5111790.1"
                     /translation="MDPVRSMLFTPGNRLDMLGKAVHSGTDAVIVDLEDSVSLDNKSE
                     ARANLARLPDSPVPYYVRTNAIETGLLWDDVVAAGASPAVGVIIPKAEDPRVLSQVDG
                     ALTALEITSGQAPGSITVIPLIESAIGVRLTYEMALASPRVECVMFGGGEQGDLVADL
                     GVEWTPEGTGLMQARSQVLLSSRAAGVLYPMEAVFMDFRNSDGLRVECELARTLGYVG
                     KVAIHPAQIEVINDVFTPAPDVVEYQRKVLAAFEEAEAEGKASIAVDGKMVDYAVARV
                     ARAILARAEAASRGGAR"
     gene            2246..>2564
                     /locus_tag="VFU96_00660"
     CDS             2246..>2564
                     /locus_tag="VFU96_00660"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU5111791.1"
                     /translation="MHSGVVVLELGQVIAGNFGGVILADLGAEVIKIEPKTGDTARNA
                     TIAPLHDESAIHLFMNRGKKSVVLDLKDERGLEMFYRLVEQADVVIDNFRPGVMSRLG
                     IDHE"
BASE COUNT          470 a          798 c          849 g          447 t
ORIGIN      
        1 agaccacccc aggtcaccgt gcggaacacc caaaacgaca agtcgagaaa agaacggccc
       61 aactcctcga gcagaatggc cacctccacc gccccggccc cggcaccacc atgctccaca
      121 ggagtgttga tccccagcca gccctgagca gccaacttgt catagacatc acgaggagga
      181 cggcgctccc gatcacactc tcggacatac tcccggctga tctccttctc acagaactcc
      241 cggaccgtcg cccggaacaa ctcctgctcg gaagtgaaat cctgttggtt catggcccct
      301 gtcgctgcaa ccagccgatc atatatcgtg catactgcag aaaacccctg gcgatgggat
      361 tggattggtg acggcgagca tcgaaacggt gcgggatctt gctgcccggc acagtaactg
      421 gggccgctgg ggccctgatg acgagcgggg aaccctgaat ctggtcaccc ccgagatggt
      481 catggccgcc gcggggctgg tgagggcagg caagaccatc tcgatggccc ttccctacga
      541 cgaagatggg ccccaaaccg ggaacctggg ccgcttcaac cccatccact tgatgatccg
      601 ggacggcgcc gatgctctcg ccgggacatc gacccgggac ttctacggcg gcgtcgacaa
      661 acacttccgc ggcaccgacg acatcatcat catgcctctc caatcgggca cacagtggga
      721 cgcattcgga catgtcgtgt tcgaggggac gatctacaac gggtatgggg ccgagtgggt
      781 ctcgagcaaa ggagcgttga agggtgacgt cgccaacgca tcacagggaa tggtgggccg
      841 aggcgtcctc ctcgacctcc cccgcgccct cggagtcgac tggctcgagc ccggccacgc
      901 catcggcagc gccgagctgg aacgcgccga ggaacatgcc cgggtgaccg tcggcaccgg
      961 cgatttcgtc ttcgtccgaa ccggcgcaat ggccgcgtcc cgtgccgccg gcgcatgggg
     1021 tgaatacgcc gggggggagg caccaggtct cgggctggac agcgtcggct gggtggcgga
     1081 cagggacatc gctgccatcg ccagcgacac gtgggggctc gaggtccggc ccaacgagac
     1141 gccggacgtc gcacaaccct tgcacatcgt cttcatcgtc cacatggggc tgtggctcgg
     1201 cgagatcttc gatctagatc ccttggccga ggattgcgct gcggatggag cttacgagtt
     1261 catgttctgc ggcccgcccc tccctttcac ccgggcggtt ggctcgccgc tcaacccgat
     1321 ggcaatcaag tgaggtcgtg atggacccgg tcagatcgat gctgttcacc ccgggcaatc
     1381 ggctcgacat gctgggcaag gcggtccatt ccggcaccga cgccgtgatc gtcgacttgg
     1441 aggattcggt gtcgctcgac aacaagtcgg aggcccgcgc caacctcgcc cggctccccg
     1501 attcccctgt tccctactac gtgcgcacaa atgcgatcga gaccgggttg ctctgggacg
     1561 acgtcgtcgc cgcaggagct tcgcccgcgg tcggcgtgat catccccaag gccgaagacc
     1621 ccagggtgct gagccaggtc gacggggcgc tgaccgcgct ggagatcacc tcaggtcagg
     1681 ccccgggctc gatcaccgtg atccccctaa tcgaatcggc gattggcgtc aggctcacat
     1741 acgaaatggc gctggccagc ccgcgggtgg agtgtgtcat gttcggcgga ggggagcagg
     1801 gggatctcgt ggccgacctc ggcgtggaat ggacgcccga aggcaccggc ttgatgcagg
     1861 cacgctcgca ggtcttgctc agctcacgtg cggccggggt gctctatccg atggaggcgg
     1921 tgttcatgga tttccgcaac tccgacggtc tccgggtgga atgtgagctg gcccggacct
     1981 tggggtatgt gggcaaggtg gcgatccatc ctgctcagat cgaggtgatc aacgacgtct
     2041 tcaccccagc gcccgatgtc gtggagtacc agaggaaggt cttggccgcc tttgaggaag
     2101 cggaggccga ggggaaggcg tcgatcgctg tcgacggcaa gatggtggac tatgccgttg
     2161 cccgggtggc gcgggcgatc ctggctcggg ccgaggccgc ctcacgcggc ggcgctcgat
     2221 gacggctctg tctgggtctg gggggatgca ctcgggggtg gtggtcctgg aattgggtca
     2281 ggtgatcgcg ggcaattttg ggggggtgat cctggccgat cttggcgccg aggtgatcaa
     2341 gatcgagccg aaaacagggg acaccgcccg taacgccact atcgccccgc tccacgatga
     2401 gagcgcgatc cacttgttca tgaatagggg gaagaagagc gtcgtcctcg atctcaagga
     2461 cgaacgtggg ttggagatgt tctatcggct ggtggaacag gcagacgtgg tcatcgacaa
     2521 ttttcggccg ggggtgatgt cccgtctcgg tatcgaccac gagt
//
LOCUS       DASTXO010000035         4130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1586111, whole genome shotgun sequence.
ACCESSION   DASTXO010000035 DASTXO010000000
VERSION     DASTXO010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586111"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /gene="ftsW"
                     /locus_tag="VFU96_00665"
     CDS             <1..1138
                     /gene="ftsW"
                     /locus_tag="VFU96_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEU5111792.1"
                     /translation="KVRAAARSRAERLRTNNRTATALLVLAVVLVVIGLGATQSASSA
                     VAIDQSEDRFYFFKRQLVGVGIGTVALLITSRIPYRVYRRLALPGFFITVGLLVAVLA
                     VGPVIGGSQRWLPIAGFSFQPSEMAKVAIVLVLAHLFEKKHRLLTRFGHFLVPVVFTV
                     GVLGGLVILQPDLGTTIIIGAAAMAVIMASDTPLRYVLVLTVLVVLAAWVLAFGDEYR
                     VDRIVGFLDPWGNASGEGYQLIQGYYALSNGGLFGVGLGASRARWFYLPNAHTDFIFA
                     IIGEETGLIGGLTVIGLYIVLALAGWWVALRAPDRFGRMVAAGLTAWLTFQAIVNIGG
                     VLGVMPITGISLPFVSFGSTSLIVCMASLGILVNIAQAGVQPKT"
     gene            1135..2208
                     /locus_tag="VFU96_00670"
     CDS             1135..2208
                     /locus_tag="VFU96_00670"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEU5111793.1"
                     /translation="MTFLIAAAGTGGHVFPGLAVGEALLTLGVPRDSVLYVGGDRLEK
                     TVYPRAGFPFLEVEVRGLQRSMTIRNLTLPRVVWRARDAIRTAIEERQVKGVLGMGGY
                     VTIPAALAARASETDLFVAEQNAWSGLANRVASRWATRVFTSFPDTEGLERGEWVGNP
                     VRGALSNLDRATTRRQALAHYGLDPAYPVLGAVGGSLGAGVINAAVSDLVRSWEGSRI
                     QVLHLTGEGNIGRDGNLPGAVHRVQRPFEERMELFYAASDLVLARAGGGVAELTVTAT
                     PSILIPGEFGSSGHQAANAAFLKEAGAAEVVSQDRLEMLPNLVTEILLSPKKLESMRI
                     GARSIAKPEAAMTIAQAMREMVR"
     gene            2205..3563
                     /gene="murC"
                     /locus_tag="VFU96_00675"
     CDS             2205..3563
                     /gene="murC"
                     /locus_tag="VFU96_00675"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEU5111794.1"
                     /translation="MRLDFRRVHLVGAGGAGMSAIAKVLAGMGHVVSGSDQRGGSTLD
                     RLEDFGVAIHTGHHPEVARAADLVVASSAVPDRDPELVAAKDAGVPVWRRPDLLAALT
                     EGITTIGATGTHGKTTTTALLVSALKALGEDPSFIVGGDLADWNTNGHFGQTPLLVVE
                     ADEAFRTFERLRLAGLIVTNVESEHMEHFGTESDLEDAFVAVAGGVDGPVVVCADDPG
                     SREVGRRTGAITYGLDSASDWRIMDLTGHDGGTRFELVGPSARAHVSLGRPGTHVALN
                     AAGALALLGSMGRDIATAAMGLASFRGVGRRWEHRGTVSGVTLIDDYAHHPTEVAATV
                     GVGREVARGRLWAVFQPHLYSRTARFHDEFGAALSGADEVVVTDVYGSREDPVPGVSG
                     AIVVDAALRHGASAHYVPHRRDLADFLVERVEQGDLVLTMGAGDITLVPTELAIRLAE
                     RS"
     gene            3560..>4130
                     /locus_tag="VFU96_00680"
     CDS             3560..>4130
                     /locus_tag="VFU96_00680"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEU5111795.1"
                     /translation="MTTLDSLIGSGLITADVPLGPFTTYKSGGPARYLAEVPDAGALD
                     DLVASGVIADMAVLVLGRGSNLVVSDAGFDGLVIRLGQSFGGVEVDGTEVRAGGAAPL
                     AQVARTSVEAGLTGLEFFIGVPGSVGGAVRQNAGCFGTETRDRLIEASIVDLGDGIIG
                     RFGPDDLELSYRHSNVTSTRLVLGATFRSV"
BASE COUNT          630 a         1293 c         1427 g          780 t
ORIGIN      
        1 gaaggttcgg gccgcggcca ggagccgtgc cgagcggctc aggaccaaca accgcaccgc
       61 gaccgcgctg ctggtgctcg cagtggtgtt ggtggtgatc gggctcggag ccacccagtc
      121 ggcttcctcg gcggtggcca tcgaccaatc cgaagatcgc ttctacttct tcaagaggca
      181 actggtgggc gtgggcatcg ggaccgtcgc cctcctcata acgagtcgga tcccgtaccg
      241 cgtctatcgg aggctcgccc tccccggctt cttcatcacc gtgggcctgc tcgtcgcagt
      301 gctcgcagtc gggcctgtca tcggtggctc gcagcggtgg ctgccaatcg cgggattcag
      361 ctttcagcca tccgagatgg ccaaggtcgc gatcgtgctg gtgctggccc atctcttcga
      421 gaagaagcat cggctcctga cacgatttgg ccacttcctc gtccccgtgg tcttcacggt
      481 cggcgtgctc ggcggcctgg tcatcctcca gcccgatttg gggaccacga tcatcatcgg
      541 agccgcggcg atggcggtga tcatggcgag tgatacccca ctgcgatatg tgctcgtctt
      601 gaccgtgctg gtggtgcttg ccgcctgggt cctcgccttc ggcgacgaat accgggtcga
      661 tcgcatcgtt gggttcctcg acccttgggg gaacgccagc ggggagggct accagttgat
      721 tcagggctat tacgcgttgt cgaatggcgg tctcttcggg gtaggccttg gggccagccg
      781 tgctcgatgg ttctatctgc ccaatgcgca caccgacttc atcttcgcca tcatcgggga
      841 agagaccggg ctgatcgggg gtctgacggt gataggcctg tacatcgtcc tcgccctggc
      901 gggatggtgg gtcgcgctcc gggctccgga ccgcttcggc aggatggtgg ctgcaggtct
      961 gaccgcatgg ctcactttcc aggccatcgt gaacatcgga ggcgtcctgg gggtcatgcc
     1021 gataaccggc atctcgctcc cgttcgtctc gtttggcagc acctccctga tcgtgtgcat
     1081 ggcgtcactc ggcatcctcg tcaacatcgc ccaggcaggg gtgcagccga agacatgacc
     1141 tttctcatcg ccgcagccgg caccggtggc cacgtgttcc ctgggctggc cgtcggcgag
     1201 gctctcctga cactcggggt tccccgggac tcggtcttgt acgtcggggg cgatcgtctg
     1261 gagaagaccg tctacccccg ggccggattc cctttcctcg aggtggaggt caggggactg
     1321 cagaggtcga tgaccatacg aaacctgact ctgccccggg tggtctggcg ggctcgagac
     1381 gccatccgca ccgccatcga ggagaggcag gtcaaaggcg ttctcgggat ggggggatat
     1441 gtcacgattc ccgccgccct ggctgcccgc gcatcggaga ccgacttgtt cgtggccgag
     1501 cagaacgcct ggagcggtct ggccaatcgc gtcgcgtcgc gctgggcgac ccgcgtcttc
     1561 acatcgttcc ccgacaccga gggcctggag cggggagagt gggtcggcaa cccggtccgt
     1621 ggggcgctgt cgaacctgga ccgcgccaca actcgacggc aggccctcgc ccactacggt
     1681 ctcgatcccg cgtatcccgt gctcggggcg gtgggcgggt cgctgggagc aggtgtgatc
     1741 aacgcggcgg tgagtgacct ggtgaggagt tgggagggga gccggatcca ggtcttgcat
     1801 ctcaccggcg aaggcaatat cggccgggat gggaatcttc ccggtgctgt ccatcgcgtg
     1861 caacggccct tcgaagagag aatggagctg ttctacgccg ccagtgacct ggttcttgct
     1921 cgggccggtg gtggggtcgc cgagctcact gtgaccgcga ccccgtcgat cctcatcccc
     1981 ggcgaattcg ggtcctcggg tcatcaggcg gccaacgccg ccttcctgaa agaggcagga
     2041 gccgcggagg tcgtctctca ggatcgtctc gaaatgctgc ccaacctcgt aacggagatc
     2101 ctgctctcgc cgaagaagct cgagtcgatg aggatcggcg cgaggagcat cgccaaaccc
     2161 gaggcggcga tgacgattgc ccaagccatg cgggagatgg tgagatgaga ctcgattttc
     2221 ggcgcgtcca tctagtcgga gcaggcggcg ccgggatgag tgccatcgcc aaggtgttgg
     2281 ccggcatggg ccacgtggtg tccgggtcag accaacgtgg tggatcgacc ctggacaggt
     2341 tggaggactt cggcgtcgcc atccacaccg gccatcatcc cgaggtggct cgagccgccg
     2401 atctggtggt cgcctcatca gcggtgcccg atcgggaccc agagctcgtt gcagcgaagg
     2461 acgccggcgt gcctgtgtgg cgccgtcccg acctcctcgc cgcgctcacg gaggggatca
     2521 ccacgatcgg ggcaacagga acccatggga agacgacgac caccgctctc ctggtctctg
     2581 cgctgaaggc tctgggggag gatccgagct tcatcgtcgg aggggatctc gccgactgga
     2641 acaccaatgg tcatttcggt cagacgcctc tgctcgtcgt cgaggcggac gaggcattcc
     2701 ggactttcga gagactgcgg ctcgccggtc tgatcgtcac caacgtcgag tcggagcaca
     2761 tggagcattt cggcaccgag tcggatctcg aggacgcgtt cgtcgcggta gccggtggcg
     2821 tcgatgggcc tgtggtggtg tgcgccgacg atcccggctc ccgtgaagtc gggcgccgaa
     2881 ccggagccat cacatacggt ctggactcag cttccgactg gcggatcatg gatctgactg
     2941 gccatgacgg ggggacccgc ttcgagctgg tcgggccatc ggcacgtgct catgtgagtc
     3001 tgggtcggcc cggcacccac gtggcgctca atgcggccgg cgcccttgcc ttgctcggct
     3061 cgatgggacg cgacatcgcg accgccgcca tgggtctggc atcgttccgc ggtgtcggcc
     3121 gccgatggga gcacaggggc accgtgtcgg gcgtcacgct gatcgacgac tacgcccatc
     3181 atcccaccga ggtggcagcg accgtcggtg tggggagaga ggtagcgcgg gggaggctgt
     3241 gggccgtctt ccagccgcat ctgtacagca ggacggcgag attccacgat gagttcgggg
     3301 ccgcgctcag cggggcggac gaggtcgtcg tcacagacgt ctacgggtca cgggaggatc
     3361 cggtcccagg ggtcagtggt gcgatcgtcg tcgacgcggc gcttcgtcac ggtgccagtg
     3421 ctcactatgt cccccaccgt cgcgatctcg ccgacttcct ggtcgagcgc gtcgaacaag
     3481 gagatctggt cctcacgatg ggagcgggag acatcacgct ggtgcccaca gagttggcca
     3541 tacgcctggc ggagcgatcg tgaccaccct cgattccctc atcggctctg ggctgatcac
     3601 cgcggacgtg ccgctaggac ccttcaccac ctacaagtcg gggggccctg cccgttatct
     3661 ggcagaggtt ccagacgcag gggcactgga cgacctggtc gcgtcgggtg tgatcgccga
     3721 catggcggtg ctcgtcctgg gccgcggctc gaaccttgtt gtctccgacg ccgggttcga
     3781 tgggctggtg atccgcctcg gacagtcctt cggtggcgtc gaggtagacg ggaccgaggt
     3841 ccgtgccggc ggtgccgccc ctctggcaca ggtggcacga accagtgtgg aggccggtct
     3901 gaccgggctc gagttcttca tcggtgtccc cggctcggtt gggggagcgg ttcgccagaa
     3961 cgcggggtgc ttcggcacgg agacacgaga ccgcctcatc gaggcctcga tcgtggatct
     4021 gggggatggg atcatcgggc gattcggccc cgatgacctc gagttgtcat accggcattc
     4081 gaatgtcacc tcgacccggc tggtgttggg agccacgttc cggtccgtcc
//
LOCUS       DASTXO010000036         3627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_75712, whole genome shotgun sequence.
ACCESSION   DASTXO010000036 DASTXO010000000
VERSION     DASTXO010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75712"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /gene="cgtA"
                     /locus_tag="VFU96_00685"
     CDS             <1..939
                     /gene="cgtA"
                     /locus_tag="VFU96_00685"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HEU5111796.1"
                     /translation="DLVEEGQVIEAVKGGRGGRGNAAFVNPSNRAPSVAEQGEYGRAE
                     WLTLELKLLADAALIGFPNAGKSTFISKVSAAKPKIADYPFTTLVPNLGVVSFDGREF
                     IMADIPGLIEGAASGKGLGHEFLRHVERARVLVILLDPSPMQERSPEEQLQILRREIA
                     DYSPEIAARPSIVLLNKSDLAEISVLAGDLGASPVSAVSGEGMRESLHAIADLVDEAE
                     RIAPQRKGFVLHRPLGPTFTVRREDGGWRIEVLAAERAVAFADLTLPEAADMAAWRLS
                     RLGVDQALLEAGAIEGDEVRIGEISFEFIPERDEEE"
     gene            complement(965..1291)
                     /locus_tag="VFU96_00690"
     CDS             complement(965..1291)
                     /locus_tag="VFU96_00690"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEU5111797.1"
                     /translation="MDALRQALINRKKELEVELARLTAPPTTGAAVSFGKRVGDGTAE
                     AVERLSTTATARSIAHSIDDVDRALAKLEVGTYGICDECGAAIGADRLDALPAASRCV
                     ECAGRR"
     gene            1347..2210
                     /locus_tag="VFU96_00695"
     CDS             1347..2210
                     /locus_tag="VFU96_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU5111798.1"
                     /translation="MTLDSPRAVAEALRSVDYLPDDRISQVVFLADRLDKPILVEGPA
                     GVGKTELAKSLAKVTGRDLIRLQCYEGLDEAKALYEWNYKKQLLRIQADVDHDWEDIE
                     QDIFSEDFLLTRPLLEAIRSENPVVLLIDEVDRVEVETEALLLEILSDFQVSIPELGT
                     MEAKTQPIVVLTSNNTRELSEALKRRCLFLHIDYPDAQREQEILAARVPGLSEHLAAQ
                     VAKVARSIRSLDLKKAPSVSETIDWARTLLALSIEDVDEAILGDTLHVLLKYQADIEK
                     AAKELTSSPPT"
     gene            2212..>3627
                     /locus_tag="VFU96_00700"
     CDS             2212..>3627
                     /locus_tag="VFU96_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU5111799.1"
                     /translation="MLGIITGFIDELRAAGVPVSMVEAIDGMRAVEAVEIGDRIALRE
                     TLRATLIKNLRHESAFDTAFDVYFASVPQQPPEPGGPGEHPAQPDGNRSDFDEPRPFD
                     LDQLLEALLEALAGLDDEAIRRGARTAVDELAGMEPGRPVGGTYYLYRTLRRLDLADL
                     EERLISALTDDQDLSEFDRRLLREEVEARIERLREEIEEEIRRRLVADRGRDAVARTL
                     RRPLIEDVDLMHATTKDLHEMEDAIGPLTRKLAARLARRRRMRAGRLDFRRTMRKSLA
                     TGGVPADPQFRHPRPHRPEVWLLCDISGSMATFSRFTLQFTHAMSTHFSKLRSFAFVD
                     TIDEVSDFFGPGVDFGSALRRVTTEAEVVWLDGHSDYGNSLETFHARYGRQLTPRTTV
                     IITGDARNNYRPPRDRVLGDIADAARALVWLNPEPEAYWDTGDSAMSHYAAHCTSVHE
                     VRTLRQLEQFIEELTLPGPRPL"
BASE COUNT          663 a         1167 c         1160 g          637 t
ORIGIN      
        1 gatctggtcg aagagggtca ggtgatcgag gctgtgaagg gcgggcgcgg gggtcgggga
       61 aacgctgctt tcgtcaaccc gtccaatcgg gccccctccg tcgccgagca aggggagtac
      121 gggcgagcgg agtggctcac actcgagctc aagctccttg cagatgccgc cctcatcggc
      181 tttcccaacg caggcaaatc cacgttcatc tccaaggtgt cggcggccaa acccaagatc
      241 gctgactacc ccttcacgac gctggtgccg aatctgggcg ttgtcagctt cgacgggcgg
      301 gaattcatca tggcggatat tccgggtctc atcgagggag cagcgtccgg caaaggtctc
      361 ggccacgagt tccttcgtca cgtcgagcgg gcacgcgtct tggtcatcct cctcgatccg
      421 tcgccgatgc aggaacgatc gccggaggag cagttacaga tcctgagacg cgagatcgcc
      481 gactattcgc ccgagatcgc ggcacggccg agcatcgtcc tactcaacaa gtcggacctc
      541 gccgaaatca gcgtcctcgc aggtgaccta ggcgcatccc cagtatcggc ggtcagcggg
      601 gaaggcatgc gtgagtcgtt gcacgccatc gcggacctgg tcgacgaagc ggagcggatc
      661 gcaccccagc gaaagggttt cgtgcttcat cgccccctgg gcccaacgtt cacggtgcga
      721 cgagaagatg ggggctggcg catcgaagtc ctcgcggccg agcgagccgt cgcctttgcc
      781 gatctgacac ttcccgaagc ggccgacatg gcggcctggc gcctctcccg gcttggcgtg
      841 gatcaagcgc tgctggaggc gggcgcgatc gagggcgacg aggtcaggat cggtgagatc
      901 tcgttcgaat tcataccgga gagggatgaa gaggaataat ggacttggcg ggcgactgag
      961 cccgtcaccg gcgtccggcg cactcgacgc agcgcgaggc cgcagggagg gcatcgagtc
     1021 ggtcggcgcc gatggccgcc ccgcactcgt cgcaaatccc gtaagtgcct acctcgagct
     1081 tggccagggc ccgatccacg tcgtcgatgg agtgtgcgat cgatcgcgcc gtggccgtcg
     1141 tgctcaaccg ctcgacggcc tctgcggtcc cgtccccgac acgtttcccg aacgacacgg
     1201 cggccccggt cgtcgggggc gcggtgagtc gtgccaactc gacttcgagc tccttcttgc
     1261 gattgatgag ggcctgacgg agggcgtcca cgcaatcgaa ccttaggaag cggagtcgtc
     1321 gaggttcccc cgtacaatgg cggccgatga ctctcgacag cccgcgagca gtggccgagg
     1381 cgctgcgctc ggtcgactac ttgcccgacg accggatatc gcaagtcgtc ttcctcgccg
     1441 accgtctcga caagccgatc ctggtagaag ggccggccgg ggtcgggaag acggagctgg
     1501 ccaaatcgct ggccaaggtc accgggcgtg atctgatcag gcttcagtgc tacgagggcc
     1561 tggacgaagc caaggcccta tacgaatgga actacaagaa gcaactgctg aggatccagg
     1621 ccgatgtcga tcacgattgg gaggacatcg agcaagacat cttctccgag gacttcctgc
     1681 tcactcgccc gctgctcgag gccattcgct ccgagaatcc cgtggtactt ctcatcgacg
     1741 aagtggaccg ggtcgaggtc gagaccgagg cgttgctcct cgagatcctt tccgacttcc
     1801 aggtctccat ccccgaattg gggacgatgg aggcgaagac tcagcccatt gtggtcctca
     1861 cctccaacaa caccagggag ctgagtgagg ctctcaagcg gcgctgtctg ttccttcaca
     1921 tcgactatcc cgatgcgcag agggaacagg agatcctcgc ggcacgagtc ccggggctgt
     1981 ccgagcatct ggccgcacag gtggccaaag tggcgcggtc gatccgaagt ctcgacctga
     2041 agaaagctcc gtctgtctca gagacgatcg actgggcccg gaccctcctc gctctctcaa
     2101 tcgaggacgt cgacgaggcg atcctcgggg acacgctgca tgtccttctc aagtatcagg
     2161 cagacatcga gaaagcagcg aaggagctga catcgagtcc tcccacctga catgctcggg
     2221 ataatcaccg gcttcatcga cgaactgaga gcagccggcg tcccggtgtc catggtggag
     2281 gccatcgacg ggatgcgggc cgtcgaggcc gtcgagatcg gcgaccggat cgcccttcgc
     2341 gagacattgc gtgcgacgtt gatcaagaac ctgcgtcacg agagtgcctt tgacaccgcg
     2401 ttcgatgtct atttcgcttc ggttccccag cagccaccgg aaccaggcgg tcctggggag
     2461 cacccggcac agccagacgg caatcgatcc gatttcgatg agccgcgacc gttcgacctc
     2521 gaccagttgc tcgaggcgct cctcgaggcg ctggccggtc tggacgatga ggcgattcgg
     2581 cgtggcgctc gcaccgctgt cgacgagctg gctgggatgg aaccaggccg gccggtcggc
     2641 gggacgtact acctgtaccg gacgctgcgt cggctggacc tggccgattt ggaggagaga
     2701 ttgatctcgg ctctcaccga cgaccaggac ctctcagaat tcgatcggcg tctgcttcgt
     2761 gaggaggtgg aagccaggat cgagcgactt cgagaggaga tcgaggagga gatccggcgc
     2821 cgcctggtcg ccgatcgggg acgggacgcc gtggctcgca cgctgcgtcg ccccctcatc
     2881 gaggacgtcg acctgatgca cgcgaccacc aaggacctcc acgagatgga ggacgccatc
     2941 gggccactca ccaggaagct ggcggctcga cttgcccgac gtcgtcgaat gcgcgcgggg
     3001 aggctcgact tccgtcgcac gatgcggaaa tctctggcca cgggcggcgt cccggcagat
     3061 ccacagttcc gccatccccg gccccaccga ccggaggtct ggctgctctg tgacatcagc
     3121 gggtcgatgg cgacgttctc caggttcacc ctccagttca cgcatgccat gtcgactcac
     3181 ttctcgaagc tccggtcatt tgccttcgtc gacacaatcg acgaggtcag cgatttcttc
     3241 ggaccaggcg tggacttcgg ctcggcgtta cggcgggtca ccaccgaggc cgaggtggtg
     3301 tggctggacg ggcattccga ctacgggaac tccctggaga cgtttcacgc ccgctacggc
     3361 cgtcaactca cccctcgcac caccgtgatc atcaccggcg atgcccgcaa caactaccgc
     3421 ccgccccgtg accgggtgct gggcgacatc gccgatgcgg ctcgtgccct ggtttggctc
     3481 aaccctgagc ctgaggcata ttgggacacc ggcgattccg ccatgtcgca ctatgccgcc
     3541 cattgcacct cggtccatga ggtgcggacc ttgcgtcagc tcgagcaatt catcgaggaa
     3601 ttgaccctcc ccggcccccg tccgctc
//
LOCUS       DASTXO010000037         4947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1807234, whole genome shotgun sequence.
ACCESSION   DASTXO010000037 DASTXO010000000
VERSION     DASTXO010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1807234"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VFU96_00705"
     CDS             <1..447
                     /locus_tag="VFU96_00705"
                     /inference="COORDINATES: protein motif:HMM:NF037014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEU5111800.1"
                     /translation="VYKLVAVVDRGEWQPAIKVSDTPAKVVNPGRKRVHRLYDERGLA
                     TVDLMSLADEDLQFPLGVRHHRETGIGRNLDESRVSVTEELLEPATLTAPSDDPAVVA
                     EARKRRVSDLERLDPGVKRLVNPHVYHVSITRRLADLKTRLIEETI"
     gene            complement(444..701)
                     /locus_tag="VFU96_00710"
     CDS             complement(444..701)
                     /locus_tag="VFU96_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111801.1"
                     /translation="MKSHRFDAISFISGLVVALVGLVFLVPQTPVDLIDAVTSLGAWF
                     WPVLLLAIGVAILVPVFLTRRDEDDEPGALVAQSGTSDDLP"
     gene            complement(722..1933)
                     /locus_tag="VFU96_00715"
     CDS             complement(722..1933)
                     /locus_tag="VFU96_00715"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU5111802.1"
                     /translation="MEQTNTTEAPEAPVVTRELVRPREGRIVAGVAQGFANRYDLPVL
                     LIRAIFVLLTFAGGLGVALYAAGWFLIRSSDETDTPAQRIFSGASTSRSWLGIALVFV
                     AILILLDNFTFLSTGIVWSVGLLVVGVLLYTGDLPRLVRNQGADEQEGVQQMTTTDAP
                     VAAPMAPVEPLAPSGPSDAGGALPPTPTPTPPVLPPPPPKPKETSYLGRITIGVVLVS
                     LGILAILDNIPGVPIEPQPRHYIALATVIIGLGLIVGGFVGRARWLILIGALLVPTLL
                     FSPVFEYNWNSEEFDRTVSPDEFAELEDSYSLDVGNLVIDLTDLPWDGETVDLEARVD
                     AGNIEIWIPLGVGLEGEAMVDIGRVGAFGRETSGLGNPTLQFDRPGPEGTVVLDASVD
                     VGNIDIQVVGR"
     gene            2008..3219
                     /locus_tag="VFU96_00720"
     CDS             2008..3219
                     /locus_tag="VFU96_00720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU5111803.1"
                     /translation="MSLIEVGARRRDHFIVTQIEQLRFRRRGRDRLVAGVAGGLADTL
                     GVSDGFVRAAFITLSMVWGLGVLVYLALWLMALDRVEDTVPEPVAAHQAVGLGLAFGG
                     VMLFVGSLGWWPSNALVLTVGALAFGTAALTDRSMPGPLAALVDPSVDRPGRLRMIFG
                     VALLIGGLAVFSSSVGPISGIGPVVLAVALTGFGILIAFGPWVARLASDLGTERRERI
                     RQEERAEMAAHLHDSVLQTLALIQRSDDPARMSILARHQESELRDWLYGNVPIDGADL
                     LSTALKASARRIEADHQVPIDVVVVGDHPLNEDTRALLGAANEAMVNAAKHSGAERIS
                     LYFEAEDDELIVYVTDQGKGFELTGVAGDRRGITESIKARVERAGGEADIESEPGEGT
                     EVVLRMPVAAG"
     gene            3216..3851
                     /locus_tag="VFU96_00725"
     CDS             3216..3851
                     /locus_tag="VFU96_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018583915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5111804.1"
                     /translation="MRVKVYLVDDHRLFLAGVRAELSEEFAIVGSAYDVDTAMEEIRG
                     LEPDVVLLDVHMPDGGGIVVVESVGETNPDVKFLALSVSDQAEDVIGMIRAGARGYVT
                     KTIEPADLADAVRRIDAGDAVFSPRLAGFVLDAFAGVMPTDDDPELDQLTTREKDVLR
                     LIARGFAYKQVARKLNISIKTVETHVSSVLRKLQLSNRHELARWASKRRIV"
     gene            complement(3869..4327)
                     /locus_tag="VFU96_00730"
     CDS             complement(3869..4327)
                     /locus_tag="VFU96_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111805.1"
                     /translation="MLGFIFRLVRFGVIALLGAALTAKFVLQSHATEDTEEIDLVNIF
                     GGSKLISAADPFFGGKVTTVFGGTLIDLRRVVPAPTGIYLDILVAFGGLSLVVPPGWR
                     VVFDGTVTAGRVEDLTRPVTDPDAPIVRIGGLVALGGVEATTRPMVEAVA"
     gene            complement(4337..>4947)
                     /locus_tag="VFU96_00735"
     CDS             complement(4337..>4947)
                     /locus_tag="VFU96_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEU5111806.1"
                     /translation="DAVSDALDLWDYRRRVHDIYRRVREGGAGEETWTEWVAARDALF
                     ADHPQSPIDDREAFFGLSYYEYDPSWRTTGRFMPVDDQMAILDHSAEGSTRFIIIGTV
                     SFELAGHDWRLEVLWLEAYGGGIFLPFRDGGNGAATYGGGRYLLDTVKGADLGHEDDQ
                     IILDFNYAYHPSCVHSYRWSCPLAPPANRIEVAVEAGERLAT"
BASE COUNT          910 a         1506 c         1665 g          866 t
ORIGIN      
        1 gtctacaaac tggtagcggt cgtggatagg ggcgagtggc agccggcgat caaggtctcg
       61 gacacgccgg caaaggtcgt caacccgggt cgcaagcgcg ttcatcgtct ctacgacgaa
      121 cgcgggctcg caacggtcga cctcatgtca ttggctgacg aggacctgca gttccccctc
      181 ggcgtgcgcc atcaccgcga gacggggatc ggccggaacc tcgacgagtc acgggtctcc
      241 gtgaccgagg agctcttgga accggcgaca ctgaccgcac cttcggatga ccctgctgtg
      301 gtcgccgagg cgagaaaacg ccgtgtgtcc gacctggagc gtctcgaccc gggggtgaag
      361 cgcctggtca acccgcacgt ctatcacgtg tcgatcacca ggcgcctcgc cgatctaaag
      421 actcggctca tcgaagagac gatctaggga aggtcgtcgc tcgttccgga ctgagcgacg
      481 agggcaccgg gctcatcgtc ctcatcgcgc cgggtcagga acaccggcac caggatcgcc
      541 accccgattg cgaggaggag aaccggccag aaccaggctc cgagcgaagt caccgcgtcg
      601 atcaggtcga cgggggtctg aggcacaagg aagacgagcc cgacaagggc cacgaccagc
      661 ccggagatga aggagatggc atcgaatctg tgtgatttca tgggtctgtt tcctttcggg
      721 ttcaacgtcc gacgacctgg atgtcgatgt tgcccacatc caccgaggcg tccaggacga
      781 ccgtcccctc aggaccgggt ctatcgaact ggagggttgg gttgcccaaa cccgatgtct
      841 ctctgccgaa ggcgccgact cgaccgatat cgaccatggc ctcaccctcg agcccgacac
      901 caaggggtat ccagatctcg atgttgccgg cgtccacccg ggcctccaga tcgacggtct
      961 cgccgtccca ggggaggtcg gtcaggtcga tgaccagatt gcccacgtcg agggagtagc
     1021 tgtcctccag ctcggcaaac tcatcgggtg acacagttcg atcgaactct tcagagttcc
     1081 agttgtactc gaagacgggg gagaagagca gcgtcggcac cagcagggcg ccgatcagga
     1141 taagccacct tgcccgtccc acgaagccac cgacgattag gcccaatccg atgatgacag
     1201 tggccagggc aatgtaatgg cgaggctggg gctcgatggg aacccccggg atgttgtcca
     1261 ggatggcgag gatgccaagg ctgaccagca ccacgccgat cgttatccga cccaggtaag
     1321 acgtctcctt cggcttcggc ggaggcgggg gcagaacagg cggagtcggt gttggcgtcg
     1381 gaggcaaggc gcccccggcg tcgctgggcc cggatggggc cagtggctcg accggcgcca
     1441 tcggcgccgc aacaggggca tcagtcgttg tcatttgttg aactccctcc tgttcgtcgg
     1501 ccccctgatt cctcaccagg cgcgggagat cgcccgtgta gaggaggaca cccaccacca
     1561 gcagcccaac cgaccagacg atgccggtgg agaggaaggt gaagttgtcg aggaggatga
     1621 ggatggcgac aaagaccaga gcgattccga gccaggatct cgacgtcgat gcgccggaga
     1681 agatccgctg ggcgggtgtg tcggtctcgt cactcgagcg gatgagaaac cacccggctg
     1741 cgtagagggc gaccccgagg ccgccggcga aggtgagcag gacgaagatc gcccgaatca
     1801 gcaggaccgg aaggtcgtag cgattggcga acccctgggc gaccccggcc acgatccgac
     1861 cctcacgggg gcggacgagt tcccgggtga ccacaggggc ctcaggggcc tcggttgtgt
     1921 tggtctgttc cattcctcag attcgaccgt ttcacgcccc gatcttcaat cggggacgac
     1981 cctgaggtat ccccgaggtt ctcggggatg tcccttattg aggttggagc ccgccgccgc
     2041 gatcatttca tcgtgacgca aatcgagcag ctccgtttcc ggcgtcgggg acgcgatcgc
     2101 ctcgtcgccg gcgttgccgg gggcctcgcg gacaccctcg gagtcagcga tgggttcgtc
     2161 cgcgccgcat tcataacgct cagcatggtc tggggtctcg gggtgctcgt ctatctggcg
     2221 ttgtggctga tggcgctgga tcgggtcgaa gacaccgtcc ccgagccggt ggcggcccac
     2281 caggcggtag gcctgggctt ggccttcggt ggagtgatgc tctttgtcgg gtcgctgggt
     2341 tggtggccat cgaacgctct cgtcctcact gtgggggctc ttgcctttgg cacggcggcc
     2401 ctgaccgatc ggagcatgcc gggtcccctc gccgccctgg tggacccgag tgtcgaccgg
     2461 cccgggcgac ttcgcatgat cttcggcgtt gcgctactga tcggcggtct tgccgttttc
     2521 tcctcgagcg tggggccgat ttccgggatc gggccggtgg tcctggccgt ggccttgacc
     2581 gggttcggga tactgattgc tttcgggccc tgggtcgccc gtctcgccag tgaccttggc
     2641 acagagcggc gggagcggat ccgtcaagag gagcgcgcgg aaatggcggc ccatctccac
     2701 gactcggtgt tgcagacact cgcgctgatc cagcgaagtg acgacccagc ccggatgtcg
     2761 atactggctc gtcatcagga atccgagcta cgtgactggc tgtacgggaa tgtgcctatc
     2821 gatggcgcgg atctcctctc caccgccctc aaggcatccg ctcgcaggat cgaggccgac
     2881 caccaggtgc ctatcgacgt cgtcgtcgtt ggagaccatc ccttgaacga ggacacccgg
     2941 gctctcctag gcgcggccaa tgaggcgatg gtcaatgcgg cgaagcactc tggagccgag
     3001 cgcatctcgc tctatttcga ggccgaggac gatgagctca tcgtctacgt gaccgaccag
     3061 ggcaagggct tcgagctcac cggcgttgcc ggcgatcggc gtggcatcac agagtcgatc
     3121 aaggcgagag tggaacgggc agggggagag gcggacatcg agagcgagcc aggtgaaggc
     3181 accgaggttg tgctcaggat gccggtggcc gcaggatgag agtgaaggtc tacctggtcg
     3241 acgatcatcg gctcttcctg gccggggtga gggcagagct ctcagaggag ttcgcgatcg
     3301 tgggctccgc ctacgacgtc gacacggcga tggaagagat acgagggctc gagcccgacg
     3361 tcgtcctgct cgatgtccat atgcccgacg gtggtgggat cgttgtggtg gagagcgtgg
     3421 gtgagacgaa cccggacgtg aaattcctcg ccctgtcggt gtccgatcag gcagaagacg
     3481 tgatcgggat gatcagggcg ggtgcccgag gatatgtgac caagaccatc gagcccgccg
     3541 acctggctga cgccgtgcgt cgaatcgatg ccggcgacgc ggtgttctcg ccacgtctcg
     3601 ctggtttcgt cctcgacgcc tttgccggcg tgatgcccac tgatgatgat cccgagctcg
     3661 accagctcac cacgagggag aaggacgtcc tccgtttgat cgcaagggga tttgcctaca
     3721 agcaggtagc ccggaagctc aacatctcga tcaagacagt ggaaacccat gtctcgtcgg
     3781 tgttgcgaaa gctccagctg tccaaccgcc acgagctggc gcgatgggca tcgaagcgaa
     3841 ggattgtctg aggctgcgct ctgcgttctt acgcaacggc ctcgaccata gggcgagtgg
     3901 tcgcctcgac cccacctaat gcgaccaggc ccccgatccg cacgatcggg gcatccgggt
     3961 cggtcacagg gcgggttaga tcttcgaccc ggccggcggt gacggtgcca tcgaacacga
     4021 ccctccatcc cggcgggaca accaggctga ggccgccgaa cgcgaccagg atgtcgaggt
     4081 agatcccggt cggggcgggg accacccgac gcaggtcgat gagggtgcca ccgaacaccg
     4141 tggtcacttt ccctccgaag aagggatctg ccgcggagat cagcttggag ccgccgaaga
     4201 tgttgacgag atcgatttcc tcggtgtcct cggtggcatg agattggagg acgaacttgg
     4261 ccgtaagggc agcgccgagt agggcgatga cgccgaagcg gaccagtctg aagatgaagc
     4321 cgagcatctt cctaccttac gtcgccaggc gctcgccggc ctcaacggcc acctcgatcc
     4381 ggttggcagg tggggccaag ggacacgacc agcggtagga gtgcacgcac gacgggtggt
     4441 aggcgtagtt gaagtcgagg atgatctggt cgtcctcgtg gccgaggtcg gcgcccttca
     4501 ccgtatccag gagatagcgc ccgccgccgt aggtggcggc gccgttgccg ccgtcgcgaa
     4561 acgggaggaa gatcccgccg ccataggctt cgagccagag cacctcgagc cgccagtcgt
     4621 gacccgccaa ctcgaacgac actgtcccga tgatgatgaa tcgcgtcgag ccttctgcgg
     4681 agtggtcgag gatcgccatc tggtcgtcga ctggcatgaa tcgtccggtg gttcgccagc
     4741 tcgggtcgta ctcgtagtag gagaggccga agaaggcttc ccggtcatcg atcggagatt
     4801 gggggtgatc ggcgaagagc gcatctcggg cggcgaccca ttcagtccag gtctcttctc
     4861 cggcaccacc ctcccgaacc cggcggtaaa tgtcgtggac ccgccgtctg taatcccaga
     4921 gatcgagtgc gtcgctcacg gcatcaa
//
LOCUS       DASTXO010000038         3170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1733512, whole genome shotgun sequence.
ACCESSION   DASTXO010000038 DASTXO010000000
VERSION     DASTXO010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1733512"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VFU96_00740"
     CDS             <1..425
                     /locus_tag="VFU96_00740"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5111807.1"
                     /translation="ALSSIRESVAGWHQASLLDELAVARDALAAAGAEFETLGDFEID
                     LAPSVENVLALGLREAVTNILRHAHAGTVEARIDSQDGGVSLTVTDDGIGPTKGNGTG
                     IQGMRERVIAAGGTLTFGPGATGGTELRIDMPFGGSSP"
     gene            422..1021
                     /locus_tag="VFU96_00745"
     CDS             422..1021
                     /locus_tag="VFU96_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5111808.1"
                     /translation="MIRVVLAEDQAMVRGAFAGLLDLEPDIEVVAVAEDGEQGLAAVA
                     EHEPDILLTDIEMPGMTGLELASRLKDHPTVRVVIVTTFARSGYLRRALDAGVSGYVL
                     KDSPIEELADTLRRVAAGNRVIAPELAVSAWTGSDPLTDREREVLREVGKGHPNVEIA
                     QRLHLAEGTVRNYLSSAIVKLGARNRTEAFAKAQEMGYL"
     gene            complement(1098..1910)
                     /locus_tag="VFU96_00750"
     CDS             complement(1098..1910)
                     /locus_tag="VFU96_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5111809.1"
                     /translation="MLKQVAEGVLVHQSELLQNNSVVVQGRAGVLLIDPGITGDEMAC
                     LANDLRGLGQPVVAGFSTHPDWDHVLWHTDLGETPRYGTARCAAHIRDLLSDADWKAR
                     VAEGLPPEIAEEIPLDLFGLITGLPAETSQIPWEGPRVRIIEHPAHAQGHAALLIEES
                     RVLVAGDMLSDILIPFPDLGAANPIEDYLVGLRLLEGVADDVGVLIPGHGSVGGADQV
                     RTRITQDRAYLQALRDGHASDDLRISPSATFGNDWLPGVHEWQQQQLAKENM"
     gene            complement(1927..2787)
                     /locus_tag="VFU96_00755"
     CDS             complement(1927..2787)
                     /locus_tag="VFU96_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU5111810.1"
                     /translation="MSSGILLAFSSALAYGAADFIGGAGARRHSSWQVVLVGQAAGAL
                     VMLGAGLLLPGSPSTPDFAWALLAGVGSATGSIFLFRGLAHGRMGLVAPISAVGAAFL
                     PVLVGAALGERPGWLIWVGIVAALPGIWLVSRDATSDRQGVGVDRTAATRRALVDGVL
                     AGGGFGILFVALAQISDNAGLLPLAANQLVGAILTLAVATGLSQPWQPRRGVVAWGSA
                     SGVLGALGTLAFMVATGTTSLGIAGVLASLYPAVTVLLAASLLGERIGTGQRTGIGIC
                     TVAVAVMALA"
     gene            2873..>3170
                     /locus_tag="VFU96_00760"
     CDS             2873..>3170
                     /locus_tag="VFU96_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020511187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU5111811.1"
                     /translation="MTVEWAGGGPDLLVTLERGNGPLRSQIQDQLRAAIRQRRLEAGE
                     RLPSTRRLAEQLGVSRGTIVEVYEQLLAEGYVESAVGSGTRVAGMKAGGKARQEP"
BASE COUNT          615 a         1013 c         1051 g          491 t
ORIGIN      
        1 atgcactgtc ttcgatcagg gaaagcgttg ctggatggca tcaggcgtcg ctgctcgacg
       61 agctcgccgt cgcacgcgat gcgctcgcgg cggcgggagc cgagtttgag acgctcggag
      121 acttcgaaat cgatctggca ccttcggtcg agaacgttct ggcattgggg cttcgcgagg
      181 cggtcacgaa cattctgcgc catgcccacg caggcacagt tgaggcccga atcgacagcc
      241 aggacggagg agtatcacta acggtcactg acgacgggat cgggcccacc aagggaaacg
      301 gcacgggaat tcaggggatg cgtgagcgtg tgatcgccgc agggggaacc ctcacattcg
      361 gacctggggc gacaggcggg acagagctac ggatcgacat gccattcggt ggaagctctc
      421 catgatccgc gtcgtcctcg ccgaagacca ggccatggtg cgaggagcct ttgcgggact
      481 cctcgacctc gaaccggaca tcgaggtagt cgctgtcgcc gaagacggcg agcagggttt
      541 ggcagctgtc gcagagcatg agccggacat ccttctcacc gacatcgaaa tgccaggaat
      601 gacgggactc gagctggcgt cccgtctcaa ggaccatccg acggttcgag tcgtgatcgt
      661 gaccaccttc gcccgaagtg ggtatctgcg gcgggcactc gatgccgggg tgtctggata
      721 cgttctcaag gactcaccca tcgaggagct ggccgacacc ctccggcggg tggcagcagg
      781 caaccgggtg atcgccccgg agctcgcagt atctgcgtgg accggcagcg acccgctcac
      841 tgaccgggag cgagaagtct tgcgagaggt cggtaaagga catcccaacg tggagatcgc
      901 gcaaaggctc cacctcgccg aaggaaccgt acgcaactac ctgtcatccg ccatcgtcaa
      961 gctcggggcc cgaaaccgta ccgaggcgtt cgccaaggcc caggaaatgg gatacctgta
     1021 gccgggccgg ctgctggaac cgtaggcccc acctcacatg ccctcaggta ggtcgcgtca
     1081 cactcactaa ccacgtctca catgttctcc ttggcgagct gctgttgctg ccattcatgc
     1141 acgccgggca gccaatcatt gccgaacgtg gccgatgggc tgatgcgcag gtcatcggaa
     1201 gcatggccgt cacgcaaggc ttggaggtac gcccgatcct gagtgatccg tgtccgtacc
     1261 tgatcggctc cgccaacgga cccgtgaccg gggattagga cacccacgtc gtccgccacg
     1321 ccctcgagaa gccgcagccc aacgaggtag tcctcgatcg gattcgcggc gcccaggtcg
     1381 gggaacggaa tcaggatgtc agacagcatg tcgccggcga cgagaacgcg gctctcctcg
     1441 atcaacagcg ccgcatggcc ctgggcatgc gccggatgct cgatgatccg gactcgaggg
     1501 ccctcccacg ggatctggga agtctcggcc ggcaaaccgg tgatgaggcc gaacaggtcg
     1561 agcggtatct cctcggcgat ttccggtggg agcccctcgg cgacgcgggc cttccagtcc
     1621 gcgtccgaca acagatctcg gatgtgggcc gcgcagcggg ctgtgccgta acgaggggtt
     1681 tcgccgaggt cggtgtgcca gagcacgtga tcccaatcag gatgcgtcga gaagcctgcc
     1741 acaacgggct ggcccaaccc gcgcaggtcg ttcgcgaggc aggccatttc gtcgccggtt
     1801 atcccggggt cgatgagcaa tacgccggcc cgcccttgga caacaaccga gttgttctgg
     1861 agtagctcgc tctggtggac cagcacaccc tccgcgactt gcttcagcat gggtggcttc
     1921 ttccgctcat gcgagtgcca tcaccgcgac tgcaacggtg caaattccga ttcccgttcg
     1981 ttgtccggtg ccgatccgct ctccgagcaa gctcgccgca aggagcacgg tgacggccgg
     2041 atagagggag gcgagcaccc cggcgatgcc gaggctggtc gttccggtcg cgaccatgaa
     2101 tgcgagggtt cccaaggcac cgagcacacc ggacgcgctg ccccaggcga cgacacctcg
     2161 gcgaggctgc cacggctgac tgaggcccgt agcaacggcc aacgtcagga tcgcccccac
     2221 aagctggttg gcggcaagcg gcagcagacc agcgttgtct gagatttggg cgagggccac
     2281 gaaaaggatc ccaaagcctc cgcctgcgag gactccgtcg acgagcgctc tccgcgtggc
     2341 cgcggtacgg tcgacgccta ccccttggcg atccgacgtg gcatcgcggg agaccaacca
     2401 gatccccggg agggcggcga caataccgac ccagatcagc caaccgggac gttcacccaa
     2461 ggctgcacct accaacaccg gaaggaacgc tgcccccacc gccgagatcg gtgcgacgag
     2521 gcccatccgt ccatgagcga gcccccggaa caagaagatg ctgccggttg ccgagccgac
     2581 tccggccagg agcgcccagg cgaagtccgg cgtggacggg ctacctggaa gcagaaggcc
     2641 tgctccgagc atgacgagcg cacctgcagc ctggccgacc agaaccacct gccacgacga
     2701 gtgccgacgc gcaccggcac cgccgatgaa gtcggcggcg ccgtacgcga gagccgatga
     2761 gaatgccagg aggattccgg agctcattgc atcgacccta cggggtgatg cggtacagcg
     2821 tgaaggtcca atccgcagac tcttgagtgg accatttagg tacgctggtg acatgacggt
     2881 ggaatgggct ggcggtggcc ccgaccttct ggtcaccctg gaacgcggca atggtccgct
     2941 caggagtcag atccaggacc agttgcgagc ggcgatccgc cagcgccggc tcgaagccgg
     3001 ggagcgcttg ccgtctaccc gacgtctggc cgagcagctc ggcgtctcac gtggcacgat
     3061 cgtcgaggtc tacgagcagc tgctggccga ggggtatgtc gagtcagcgg tcggatcggg
     3121 gacgcgggtg gccgggatga aggccggcgg caaggcccga caagagcccg
//
LOCUS       DASTXO010000039         3265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_739130, whole genome shotgun sequence.
ACCESSION   DASTXO010000039 DASTXO010000000
VERSION     DASTXO010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739130"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VFU96_00765"
     CDS             complement(<1..792)
                     /locus_tag="VFU96_00765"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111812.1"
                     /translation="MSTPRQDLGEALWRLSDLVRADERSRSFRAKAYRRAVWSLDELA
                     PDLSDPSEKALSIPGIGAGVLRLIEEFLESGRLALLARLEEVYPRDVARMRRLPRMTP
                     TILRSLKGELGVDTSTELIVAIEAGGVEALRGVGPATAERWARTLRLAPSDRAIPGFQ
                     ATVLADALTLHLSRHLGAETRPAGGVRRMDEWVESVDLVAGTEKVEAGRDLLAGTAVA
                     RLTGHGPMGELVLRTHENVEARIHLVQPSQIGRRLLLATGPDWHAA"
     gene            867..1307
                     /locus_tag="VFU96_00770"
     CDS             867..1307
                     /locus_tag="VFU96_00770"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEU5111813.1"
                     /translation="MSKFVRIVGIGAAAAAAMYLLDPDRGRTRRAKLSDQAAALARKA
                     EEKARAQAEYQKGVAKGLAHDLTEPFRPEPDFDDETLRQKVKSEAIGRWEGPKSDVEV
                     DVDSGVVTLKGNVDSENVDQLIRLVKGVPGVASVSDQLTVPQRS"
     gene            complement(1336..2295)
                     /locus_tag="VFU96_00775"
     CDS             complement(1336..2295)
                     /locus_tag="VFU96_00775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007979121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEU5111814.1"
                     /translation="MVEHGLTLSSEEHSPTRLVEIAVAAEEAGFDFVSISDHFHPWIG
                     EQGHSPFVWSVLGALSQATSDLKVGVGVTCPTVRMHPAISAHAAATAACLLDGRFTWG
                     VGTGEALNEHVLGDPWPPADVRLEMLEEAIEIVRGLWRGESFTHRGRHYTVEDARIFD
                     VPEVLPPIVVSAFGDEAVELAARIGDGLWITGLEQGVIEKYREMGGKGPVWSQLSLCW
                     DPDPEEAVDRAYRIWPNTALAGQLAQDLRTVGHMEQAAEMIDRSDIEKALPCGPDPAP
                     IIESIEKAAAMGIDHIYLHQIGDPLAGFIEFWRDEIRPKIEGL"
     gene            2574..>3265
                     /locus_tag="VFU96_00780"
     CDS             2574..>3265
                     /locus_tag="VFU96_00780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5111815.1"
                     /translation="MRLIDGRPPTSALGQVGMEIDPNSPVSIRVAARHLERCDVVILQ
                     HEYGIFGDNDGESVLEMVDSINRPLITVVHTVVRAPSDRQRRIMERLHETSRMVVLSE
                     SARSALVSSYAIPRSEVVVIPHGSLWAATPPPPGPPSRLITWGLLGPGKGLERSIEAM
                     AKLRDLDPKPHYTIVGRTHPVVARNQGLAYRRRMESMVRDLGIEDMVQFVDRYLGDDE
                     LFELVRKSHVAV"
BASE COUNT          590 a         1053 c         1042 g          580 t
ORIGIN      
        1 ggcggcgtgc caatccggtc ctgtcgccaa gagcaggcgc cgtccgatct gggagggctg
       61 gacgagatgt atccgggcct ccacgttttc gtgggttcgc agcaccaact cacccatagg
      121 cccgtgaccg gtaagtcggg caaccgccgt gcccgccagg aggtcgcgcc ctgcttcgac
      181 cttttcggtc ccggcgacga ggtcgacgga ctccacccat tcgtccatcc ttctcacgcc
      241 accggcaggt cgagtctcgg caccgaggtg gcgactcaga tggagggtga gggcatcggc
      301 gaggacggtt gcctggaagc ccgggattgc ccgatccgaa ggcgccagcc gcagggtgcg
      361 tgcccatcgc tccgccgtgg ccggcccgac tcccctcaga gcctcgacac caccggcctc
      421 gatggcgacg atcagctcgg tggaggtgtc taccccgagc tcgcctttca gggaccgaag
      481 gatggtgggg gtcatccggg gcagacgtcg catcctggcg acatcacggg gatagacctc
      541 ctcgagacgt gccaacaggg cgagtcgtcc gctttccaaa aactcctcga tcagccggag
      601 cacaccggcc ccgatgccgg ggatcgaaag tgccttctcg gatgggtcgg aaagatcggg
      661 cgccagttca tccagagacc acacggcccg gcgataggcc ttggctcgga acgaccggct
      721 tcgctcgtcc gcacgcacca ggtccgacag ccgccagagg gcttcgccca ggtcctggcg
      781 tggggtgctc aactcagagg acctgactcg ttttgcggaa ttcgggcacg ggtaatcaac
      841 aagtgcaacc cagctaagga ggatctatga gcaagtttgt gcgcattgtc ggaatcggag
      901 cagcggccgc cgccgcgatg tacctcctcg accctgatcg cggccgtact cgacgggcca
      961 agctgtccga tcaggccgcc gcactggcgc gtaaggccga ggagaaggct cgcgcccagg
     1021 cggagtacca gaagggcgtg gcgaagggtc ttgcccacga cctcactgag ccattccgac
     1081 ccgagcccga cttcgacgac gagacgcttc gccagaaggt gaagagcgag gcgatcggtc
     1141 gatgggaagg gccgaagagc gacgtcgaag tcgatgtaga cagcggcgtg gtcacgctca
     1201 aaggcaacgt cgactccgag aacgtggatc agctgatccg actggtcaag ggcgtgccgg
     1261 gtgtggcgtc ggtcagtgat cagttgaccg tcccgcagcg cagctagcgc aacctcccca
     1321 ggatcgaggg ctccatcaga gaccctcgat cttgggtcgt atctcgtcgc gccagaactc
     1381 gatgaacccg gccaatgggt cgccgatctg gtggagatag atgtggtcga tacccatggc
     1441 agccgccttc tcgatcgact cgatgattgg ggcagggtct ggcccgcacg gcagcgcctt
     1501 ctcgatgtct gagcggtcga tcatctcagc cgcctgctcc atgtggccaa cggtgcgaag
     1561 gtcttgcgcc agctgaccgg ctagggcggt attgggccag attcgatagg ccctgtccac
     1621 tgcttcctcc gggtcggggt cccagcacag cgagagctgg ctccacaccg ggcccttgcc
     1681 gcccatctcg cgatatttct cgatcactcc ctgctccagg ccggtgatcc aaagcccatc
     1741 accaattcgg gctgcgagtt ccaccgcctc gtcaccgaac gccgatacga cgatcggcgg
     1801 cagcacctcg gggacgtcga agatccttgc gtcctcgacg gtgtagtgac ggccgcggtg
     1861 cgtgaaactc tctcctcgcc ataggccgcg gacgatctcg atggcttctt cgagcatctc
     1921 gaggcggacg tcggccggtg gccacggatc tccgagaacg tgttcgttga gggcctcccc
     1981 ggttccgact ccccaagtga accggccgtc gagcagacac gccgcggtgg cagccgcatg
     2041 ggcgctgatc gcgggatgca tgcgaacagt ggggcaggtc actccaacgc cgactttcag
     2101 gtctgaggtt gcttgtgaca gggcgcccag gaccgaccag acgaacgggc tgtgaccctg
     2161 ctcgccgatc cacggatgga agtggtccga gatcgatacg aagtcgaaac ccgcctcttc
     2221 cgcggcgacc gcgatctcca ccagcctcgt tggggagtgt tcctcgctgg agagggtcag
     2281 accatgttcg accacgaccc atttttaccc aggccgaagt cgagggaaac cgaacagttc
     2341 gtgtcgtttt gaggggtcgc ggcaacgggt aatgaatgtc tctatcaaca ctcaagaaac
     2401 ggcattctca gatcatgttt gacacttcct acccccaggc ccgacgggcc tcgaccggcc
     2461 cgcgagtcgg tatcgtctcc acgttcccac cgacacggtg tgggatcgcc cggttctcaa
     2521 cagctctcgt ctcgtcgctc ggcgatgttg ccccagatct agacgttgac ctggtgcgtc
     2581 tgatcgacgg gcgcccgccg acttcggctc tcgggcaagt cgggatggag atcgatccga
     2641 acagtccggt gagcatccgg gttgcggccc gacatctcga gcgctgcgac gtggtcatcc
     2701 tccagcacga gtacggaatc tttggcgaca acgacggcga gtctgtcctc gagatggtgg
     2761 attccatcaa ccgcccgctg atcaccgtgg tccacacggt ggtcagggcg ccaagcgatc
     2821 ggcagcgacg gatcatggaa cgccttcacg agacgtcccg aatggttgtg ctctccgagt
     2881 cggcgcgttc ggccctggtc tcttcgtatg cgatcccgag gagcgaggtg gtggtcatac
     2941 cccacggttc gctctgggcg gcgacgccac caccaccggg tccgcccagt cgtctgatca
     3001 cctggggcct gctcgggccg ggcaagggcc tggagcgttc catcgaggcg atggccaagc
     3061 tccgcgacct cgatcccaag ccccactaca ccatcgtggg tcgaacccat cccgtggtcg
     3121 ctagaaacca gggcctcgcc taccggaggc gtatggagag catggtgagg gacctgggta
     3181 tcgaagacat ggtccagttc gtcgaccgct acctcggcga cgatgagctc ttcgagttgg
     3241 tgcgaaagtc ccacgtcgcg gtcat
//
LOCUS       DASTXO010000040         3658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1807449, whole genome shotgun sequence.
ACCESSION   DASTXO010000040 DASTXO010000000
VERSION     DASTXO010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1807449"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..588)
                     /locus_tag="VFU96_00785"
     CDS             complement(22..588)
                     /locus_tag="VFU96_00785"
                     /inference="COORDINATES: protein motif:HMM:NF025066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEU5111816.1"
                     /translation="MSNGRNPASCSGSRRRCGHDRHAHSFWSRCESGTASAPHRPRGL
                     QGDARSGLPAVDPGHDRGSLGWEAGVYFTFYGLDIIHNDRLESLKVASLANPAGPIQL
                     PNLLGALPGATAAATKMMKKWMSDARMPTVPEFIEMAQGLDVRFFACSTTMGVMGVEE
                     GDLIKGCEIAGAAAFLDFAADADVQLFV"
     gene            complement(530..796)
                     /locus_tag="VFU96_00790"
     CDS             complement(530..796)
                     /locus_tag="VFU96_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019625590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEU5111817.1"
                     /translation="MTSQYVKVKDERVDVELDCSGVLCPLPVYRASLALGQLSPGQVL
                     KLITTDPGALEDIPALARQTGNILLGVEREESRQLFWIEKAVRA"
     gene            complement(1215..2000)
                     /locus_tag="VFU96_00795"
     CDS             complement(1215..2000)
                     /locus_tag="VFU96_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111818.1"
                     /translation="MTRPCDDGAEPWHHEGVTKTAHLRVYTPESSEDGDPVPGFVRSY
                     CLLSESEGDSDWVVDWNGQRMVCPRNLRLRVLESTVAFANAFRGLGSGLIPEEAARAA
                     DNELRRYHQEHPGHRSHVLTSAWHVPVRWFTAFQPEDKEVYEVVGSPRIRLRVDLSVG
                     RKRVGHALGVLRGLQVFQGPAEELAQLSEWIDSFPGESMLELDYSTVSDLFAPQELVF
                     DDSVELVRRSIEALADGDMMTAGENYGRVVSRWAPAFSVTFSS"
     gene            2061..3143
                     /locus_tag="VFU96_00800"
     CDS             2061..3143
                     /locus_tag="VFU96_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003333073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEU5111819.1"
                     /translation="MVVFDEVARGGHEQIIYGYDHVSGLRAIIAIHSTALGPALGGTR
                     FFPYETEDDALVDVLRLAKGMTYKAAAAGLDLGGGKAVIIGNPATDKTERLLRAYGRI
                     VDSLRGRYITAEDVGTTTSDVDIIRRETRWALGSSPAEGGAGDPSPVTSRGLFAASRA
                     VADFMWGDTDLAGRRFAVQGVGKVGAAFVQLLVEARAEVIVADSNERAVTDAVETYGV
                     KGIDVEDIHTVDCDFYSPCALGGGLNESTIPRLNCQAIVGSANNQLASDDDADRIASR
                     GIVYAPDFVVNAGGLINVHDELHGYSKMRAMHRVDSIFDATKAILVASREQQLNPNQA
                     AVLLAEDRIREIGDLRRFRRSGEDRS"
     gene            3140..>3658
                     /locus_tag="VFU96_00805"
     CDS             3140..>3658
                     /locus_tag="VFU96_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU5111820.1"
                     /translation="MTSHRTYGMDFDRNLHHGELGLSDDQVVEMYRLMLTTRRIDDRL
                     FALQRQGRAPFVVGSSGHEAVQVASALALDREKDWVLPYYRDMGVALAWGFEPVEIFL
                     GAFAKKTDPMSGGRQLPGHWSSRERRVLTQSSVIGTQYPHAVGIAQAVANRGDDAVVA
                     VYGGEGSTSEGDW"
BASE COUNT          679 a         1153 c         1196 g          630 t
ORIGIN      
        1 ctcaatggcc ggctgacggg ttcagacgaa caactgaacg tctgcgtcgg cggcgaagtc
       61 gagaaacgcg gcggccccgg cgatctcgca ccccttgatc aggtctccct cttcgacgcc
      121 catgacgccc atggtcgtcg agcaggcaaa gaaccggacg tcgagcccct gtgccatctc
      181 gatgaactcg gggactgtgg gcatccgggc gtcgctcatc catttcttca tcatcttggt
      241 ggcggcggcc gtcgccccag ggagcgcccc gagcaggttg gggagttgaa tcgggcctgc
      301 cgggttggcc agcgacgcca ccttcaggct ctccagccga tcgttgtgaa tgatgtcgag
      361 gccgtagaag gtgaaataca cccctgcctc ccatcccagg ctgccgcggt cgtggccagg
      421 atcaacggcg ggtaggcctg atcgagcgtc cccttggagg ccacgagggc gatgcggcgc
      481 cgacgctgtt cctgactcac atcggctcca gaatgagtga gcgtgtcggt catgcccgca
      541 ccgccttctc gatccagaac agctggcggg attcctcccg ttcgacaccg agcaggatgt
      601 tgccggtctg cctggccagg gccgggatgt cctccagcgc ccccgggtcg gtggtgatca
      661 gcttgagcac ctgtccgggt gagagttgac ccagggcgag cgaagctcga tagaccggta
      721 gcggacagag cacacccgag cagtcgagct ccacgtcgac gcgctcgtct ttcactttga
      781 cgtattggct ggtcatggtc cgtctccttc tctcctccgg agggggccca tgacgacaaa
      841 ggcccgcatc cggtaccggt gcgggcttct gtgtctctgt ctctgctgac tcaggacatg
      901 actacccgca cggcacggcc gcacagcgac aggcgcagca gcaggtcggg ctcgtgggtg
      961 ggcaggtcat gtcgatgagg atggtaggcg ttcacagcca ggggtcaaga ccggacgccg
     1021 gttcatcaga gagttgcgag gccagtggag catttgagga ggagaaggag tccgctggga
     1081 gtagcggaaa ctggctccac tggccctcgc aaatcggaag gagggggaac agcttgtgat
     1141 tctgttcaca aatcgagaac gcatccgaaa gccatttatt cccgttgcca cgctgcactt
     1201 gcttcggaac ccgatcagct actgaaggtg accgagaagg cgggggccca gcgagagacg
     1261 acacggccgt agttctcgcc agccgtcatc atgtcaccat cggcgagagc ctcgatcgag
     1321 cgtcgcacca gctcgaccga atcgtcgaaa accagctcct gtggggcgaa caggtcggag
     1381 acggtcgagt agtcgagctc gagcatcgac tcgccaggaa agctgtcgat ccattccgaa
     1441 agctgggcga gctcctcggc aggcccctga aacacctgga ggccgcggag cacgccgagg
     1501 gcatggccaa cccgcttccg ccccacggag agatcgacac gcaaccggat ccgcggagag
     1561 cccacgactt cgtacacctc tttgtcctcg ggttggaagg cagtgaacca gcggaccggg
     1621 acatgccagg cggaggtgag gacatgggaa cgatgacccg gatgctcctg gtggtagcgg
     1681 cgaagctcgt tgtcggcggc tcgggccgcc tcctcgggaa tgaggcccga tccgaggcca
     1741 cgaaacgcgt tggcgaaggc gacagtcgac tcgaggacgc gaagacggag attgcgtggg
     1801 cacaccattc gctgcccatt ccagtccacg acccaatcgg aatcaccctc ggattcggag
     1861 agcaggcaat agctccgaac gaaccccgga acagggtcgc cgtcctcgga gctctctggc
     1921 gtgtacaccc ggagatgggc ggtcttggtc accccttcat ggtgccatgg ctcggcccca
     1981 tcgtcacacg gccgggtcac gggcgcccag gtaagattgg cccagcgtga gaaccgcccc
     2041 tcgagaacgg aggaccagac ttggtcgtct tcgatgaggt tgcccgcgga ggtcacgagc
     2101 agatcatcta cggctatgac catgtatccg gccttcgggc cataatcgcc atccactcca
     2161 cggctctggg tcccgctctc ggcgggaccc gcttttttcc atacgagacc gaggatgacg
     2221 ccctcgtcga cgtgctgcgt ctcgccaaag gaatgacata caaggcggcc gccgcaggtc
     2281 tcgatctggg aggtggcaaa gctgtgatca tcggcaaccc ggcgaccgac aagacagagc
     2341 gccttcttcg ggcctacgga aggatcgtcg actcgttgcg cgggcgctat atcaccgccg
     2401 aggatgtcgg gaccaccacg tccgacgtcg acatcatccg ccgcgagact cgttgggctc
     2461 tcggcagctc tcccgccgaa ggcggagccg gtgacccctc acccgtcacc tcgaggggcc
     2521 tgttcgccgc ctcccgggcc gtcgccgatt tcatgtgggg agacaccgat ctcgctggtc
     2581 gcaggttcgc cgttcaggga gtcggcaagg ttggagcggc cttcgtccag ctactggtcg
     2641 aggcgcgcgc agaggtcatc gtggccgact cgaacgagag ggcggtcacc gatgcggtgg
     2701 agacctatgg agtcaagggg atcgacgtgg aagacatcca cacggtcgat tgcgatttct
     2761 attcgccctg cgccctggga ggcgggctca acgagagcac aattccccgt ctcaactgcc
     2821 aggcgatcgt gggcagcgcc aataaccagc tcgccagcga cgacgacgcc gatcgcatcg
     2881 cctcccgggg catcgtctat gccccggatt tcgtggtcaa cgccggcggt ttgatcaatg
     2941 tccacgacga attgcacggc tattcgaaga tgcgggcgat gcatcgggtc gattcaatct
     3001 tcgacgcgac caaagccatc ctcgtcgcat cgagggaaca gcagctcaac ccgaaccagg
     3061 cggctgtatt gctggccgag gatcgcatcc gggagatcgg cgacctccgc cgcttccgcc
     3121 gcagcggaga ggaccgcagt tgaccagcca tcgcacctac ggcatggatt tcgaccggaa
     3181 tctccatcac ggggaattgg gcctgtcgga cgaccaggtc gtggagatgt accggctgat
     3241 gctgacgacg cggcggatcg acgaccgact cttcgccctt cagcgccagg ggcgggctcc
     3301 cttcgtcgtc ggatcctccg ggcacgaggc ggtccaggtg gcatccgccc tggccttgga
     3361 tagagagaag gactgggtcc ttccctacta ccgggacatg ggggttgcgc tggcatgggg
     3421 tttcgagccg gtggagatct tcctcggggc gtttgccaag aagaccgacc cgatgtccgg
     3481 aggccgtcaa ctgcccggcc actggtcgag tcgcgagcgg cgcgtcctga cccagtcctc
     3541 cgtcatcggg acccaatatc cccacgccgt gggcatcgcc caggcggtgg ccaaccgggg
     3601 cgacgacgcg gtcgtggccg tgtacggggg tgagggctcg acgtctgagg gggactgg
//
LOCUS       DASTXO010000041         2326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1807458, whole genome shotgun sequence.
ACCESSION   DASTXO010000041 DASTXO010000000
VERSION     DASTXO010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1807458"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="VFU96_00810"
     CDS             <1..728
                     /locus_tag="VFU96_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111821.1"
                     /translation="HDDCDSEECPDVTVQTGGGDHDDCDSEECPDVTVQGDKDDDPDC
                     TSTTTTTTTTVPTVAPPTTVPGLDLAAAGAECISDIPYLRYEIDYPPGQSATITFLNP
                     TGPDVVYEDVPLSGAVLWPGASENPPDWPGWVREDGVWVEADDGFLWARGTISVLFEV
                     NPSTMVTVSYQQGTGACADPDPDEVEGTVITPPTTPDQVDGVDVLPFTGVQDGVLAVV
                     AITALALGGLMVLSARRDEPDES"
     gene            784..2067
                     /locus_tag="VFU96_00815"
     CDS             784..2067
                     /locus_tag="VFU96_00815"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEU5111822.1"
                     /translation="MNYHDALAYLDGHIAHGIKPGLDRINRLLDDMGRPDEGYPIIHV
                     AGTNGKTSTARFATLLLVAHGLNTGTYTSPHLQKVEERLAINGRMATEEEFALASSDV
                     AAFADLRTRAGEEPNTYFELTTAAAFAFFADQAVNAAVLEVGLGGRLDATNVVDAEVC
                     VLTSIGIEHTEYLGEDVATIAGEKLAIAGPNSTLVSGPLPDQALEVAETRARDLGIHH
                     RHYGKDFSVLDAERGVGGWLVTIGGAEDTYEDLFLPLHGRYQLVNLANAVAATEALLG
                     RKLDEEAVRDAVAIATVPGRMEVLGTHPLVMVDGAHNADGVVALTESLDEEYPTTRWH
                     LVLGVMGDKNVEAMVESLEPLLAGIVVTAPHSERAVPPAALADRIRDLTDVAILEADD
                     TELAIDMARAEAGVEGAVLVAGSLYLVGEARALLV"
     gene            2124..>2326
                     /locus_tag="VFU96_00820"
     CDS             2124..>2326
                     /locus_tag="VFU96_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016330321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU5111823.1"
                     /translation="MSIESTFIMVKPDGVARGLVGEVITRFEKKGLTLERIRKLDIDE
                     DMARRHYEEHLEKQFFPELLDFIT"
BASE COUNT          447 a          736 c          745 g          398 t
ORIGIN      
        1 atcacgacga ttgtgattcc gaggagtgcc cggatgtgac ggttcagacc ggcggtgggg
       61 atcacgacga ttgtgattcc gaggagtgcc cggatgtgac ggttcagggt gataaggacg
      121 acgacccgga ctgcacgtcc acgacgacga cgaccacgac caccgtgccc acggtggcac
      181 ctccgacaac cgttcctggg ctggacttgg ctgcggctgg ggctgagtgc atcagcgaca
      241 tcccatatct caggtacgag atcgactatc ctccggggca atcggccacg atcaccttcc
      301 tcaaccccac cgggcccgac gtggtttacg aggatgtgcc tttgagcggc gccgtgctat
      361 ggcctggggc gagcgaaaat ccccctgatt ggccgggatg ggtccgcgag gatggagtct
      421 gggtggaagc cgacgacggt ttcctctggg ccaggggaac gatcagcgtc ctcttcgagg
      481 tgaacccgag cacgatggtc acggtctcct accaacaggg gacgggggcc tgcgccgacc
      541 ctgacccaga cgaggtcgaa ggaactgtga tcacgccacc aaccacgccc gaccaggtcg
      601 atggcgttga tgtcctcccc ttcaccggag tccaggatgg tgtcctggct gtggttgcga
      661 tcacagccct ggccctcggt gggctgatgg tgctcagcgc ccggagggac gaacccgacg
      721 agagctgaag gcggacgccg agataggtga caaagccccg ggattaacct cccggggctt
      781 ttcatgaact atcacgacgc tctcgcctat ctcgatgggc acatcgccca cggcatcaag
      841 cccggcctgg accgaatcaa ccggttgctc gacgacatgg ggcggccaga cgagggatac
      901 ccgatcatcc atgtggccgg gaccaacggc aagacctcca ccgcacgctt cgcaaccctg
      961 ctcctcgttg cccacggact caacacgggg acctacacgt cgccccatct ccagaaggtc
     1021 gaggagcgtc tggcgatcaa tggtcgtatg gccacagaag aggagttcgc tctcgcctcc
     1081 tcagacgtcg cagctttcgc cgacctgcgg actcgagcag gtgaggagcc caatacctac
     1141 ttcgagctca ccaccgccgc ggcattcgcg ttcttcgccg accaagcggt caacgcggcc
     1201 gtgctcgagg tgggtctggg tgggcgtctc gacgccacca acgtcgtcga cgccgaagtg
     1261 tgcgtcctca cgagcatcgg gatcgagcac accgaatacc tcggcgagga tgtggccaca
     1321 attgccggtg agaaactcgc catcgccggt ccgaattcga ccctcgtcag tgggcccctg
     1381 cccgaccaag cactggaggt cgccgagacg cgtgcccgtg acctgggcat ccaccaccgt
     1441 cactacggaa aggatttctc ggttctggac gccgagcggg gagtgggtgg atggctggtg
     1501 accataggtg gcgccgagga cacctacgag gacttgttcc ttcccctgca tggacggtat
     1561 cagttggtca acctggccaa cgccgtggcc gcaaccgaag ccttgctggg acgcaaactc
     1621 gacgaggaag cggttcgaga tgcggtggcg atcgccacgg tgccggggcg gatggaggtg
     1681 ttgggcacgc acccactcgt aatggtcgat ggagcccaca atgccgacgg tgtggttgcc
     1741 ctcaccgagt cgctcgatga ggaatacccg acgactcgct ggcacctggt cctgggggtg
     1801 atgggagaca agaacgtgga ggccatggtg gagagtctcg aacccctctt ggccgggatc
     1861 gtcgtcaccg cgccccactc ggaacgcgca gtcccaccag cggcactcgc tgatcggatc
     1921 agagatctga ccgacgtggc catactcgag gcggacgaca ccgagctggc gatcgacatg
     1981 gctcgcgccg aggccggcgt cgagggtgcg gtgctcgtgg ccggctcgct ctacctcgtt
     2041 ggcgaagcac gcgcgttgct cgtctgatcg ccctgccccg gcggggccgg ccgaacgctc
     2101 aaagcagtta ccctcccgcc cacatgtcaa tcgagagcac cttcatcatg gtcaagcccg
     2161 atggcgtcgc tcgcggcttg gtgggtgagg tcatcacacg attcgagaaa aaaggcctga
     2221 ccctggagag gatccggaag ctcgacatcg acgaggacat ggctcgtcgt cactacgagg
     2281 agcatctgga gaagcaattc ttccccgagc tgctcgactt catcac
//
LOCUS       DASTXO010000042         2435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417 k141_2359,
            whole genome shotgun sequence.
ACCESSION   DASTXO010000042 DASTXO010000000
VERSION     DASTXO010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2359"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1468
                     /locus_tag="VFU96_00825"
     CDS             <1..1468
                     /locus_tag="VFU96_00825"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111824.1"
                     /translation="TSNGDWTEGSEQLGKVLALHGGDREVRGRAIVALGSLLLLRGEL
                     EEADRCFGEARELAAASGDDVNLARALLYSGYVAFRHSHLAEAETLWEDALTRAQSGG
                     DQRLAASVLRSLAIVAGTEGRQDRAGELLDQAIGLAREAGDDQLLRLLFGSSAEMNIW
                     LGHYQVAEKAYGDALSLAAEIGDFSARPLLLAELGWVAILRGDPPTAQRLAIEAAELA
                     EDLGTPRVLAHSLRLGGEAMMRLGELTAASESLERALTVAESLNAAAEVAGVRCSQAC
                     LALEFQDWDLARAHAREAIETSGLAHTMRRTTPQWVLGMVSLLEGEIGSAGEQFLESL
                     NAAEERGAPRHEAVFCLGLAAVQTARGRSREAASLCKRALDLWVNLEDPLGVADTMVA
                     LAELGAKTEPEGAAELLGAAESLRERAGAKATPREAGQVAAISASLDLPAFEGGTAGA
                     EADEAEALAEARRIISMMEEADSAGEKRDFPVADTGNQ"
     gene            1480..2286
                     /gene="hcaB"
                     /locus_tag="VFU96_00830"
     CDS             1480..2286
                     /gene="hcaB"
                     /locus_tag="VFU96_00830"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004726042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(cis-5,6-dihydroxycyclohexa-1,
                     3-dien-1-yl)propanoate dehydrogenase"
                     /protein_id="HEU5111825.1"
                     /translation="MGWLDGDVAVITGAGSGLGRALVDRFVAEGARIVAVDRAEDRVA
                     EVEKVHGDVIAGVVADVTSAADNERAVAEGLDRFGQIDVFIGNAGIFDFGASFVDTPI
                     DRLEKGFDELFALNVKGYLLGVKAAVNALIESDGSVLLTASMSSWHAGVGGVVYTASK
                     HAVVGLVRQLAHELAPKVRVNGVAPGFMRTDIRGPKALGLGDVTSSSLPDLEQIAVAT
                     TPLRFLPRPEDYTGHFVQLASRSNAPATTGTVVACDGGLDVRGIGLPQPG"
BASE COUNT          401 a          754 c          864 g          416 t
ORIGIN      
        1 gacgtcgaac ggagactgga ccgagggatc ggagcagctc ggcaaggtgt tggccttgca
       61 cgggggcgac cgggaagttc gtggtcgtgc gatcgtcgct ctgggcagtc tcctgctgct
      121 caggggcgag ctcgaggagg ccgacagatg ctttggggag gcaagggagt tggccgcagc
      181 gtcgggcgac gacgtcaacc tggctcgtgc tctcttgtat tcgggttacg tcgcgttccg
      241 acactctcac ctggcggagg ccgaaacact ctgggaggac gccttgaccc gcgcccaaag
      301 tggcggagac cagcgcttgg cggccagcgt cctgagaagt cttgccatcg tcgccggcac
      361 cgaagggcgc caggaccgcg ccggcgagtt gttggaccag gccatcggtt tggcgcggga
      421 ggcgggagac gaccagctgc tccgcctcct gttcggctct tcggccgaga tgaacatctg
      481 gctgggccac tatcaggtgg cagagaaggc ctacggagat gcactgtccc ttgccgccga
      541 gatcggcgat ttctctgccc gcccactcct gctggccgag ttgggctggg tggcgatcct
      601 gcgtggggac ccacccaccg cacaacgcct cgccatcgaa gcggccgagc tcgccgaaga
      661 cctgggcacg cccagggtgc tcgctcattc gctacgtctc ggcggtgagg ccatgatgcg
      721 tctcggagaa ctgaccgccg cgtcggagtc tctcgagcga gccctcaccg tcgccgaaag
      781 cctgaacgct gctgccgagg tggccggggt gcgctgttcg caggcgtgcc tggctttgga
      841 gttccaggat tgggacctgg cccgagccca cgcccgggag gcgatcgaga cttcgggctt
      901 ggcccacacg atgcggcgaa ccactccgca gtgggtgttg gggatggtca gcctgctcga
      961 aggcgagatc gggtccgctg gagaacagtt cctggagagc ctgaacgcgg ccgaagagag
     1021 gggcgcacct cgccacgagg ccgttttctg cctgggtctg gccgcggtac agaccgctcg
     1081 ggggcgatca cgcgaggcgg cgagcctttg caagcgcgct ctcgacctgt gggtgaatct
     1141 ggaggacccg ctgggtgtgg ccgataccat ggtcgctctg gcggagctcg gcgcgaaaac
     1201 ggagcccgag ggagctgcgg agttgctcgg tgcagcggaa tcactccggg agcgcgccgg
     1261 ggccaaggcg actccacgtg aagcggggca agtcgcagcg atatcagcat ctcttgatct
     1321 acccgcattc gagggcggga cggccggagc agaggcggac gaagccgagg cgttggccga
     1381 ggccaggcgg atcatctcga tgatggagga ggccgattcg gctggcgaga agcgcgactt
     1441 ccctgtcgca gacaccggaa atcaatagga gggaacagca tgggatggct cgatggtgat
     1501 gtggctgtga tcaccggcgc cggatcgggg ctggggcgtg ccctggtgga tcgctttgtc
     1561 gccgaaggcg cacggattgt cgccgtcgac cgcgccgagg accgggtcgc cgaggtcgag
     1621 aaagttcacg gcgatgtcat tgccggcgtc gtcgccgacg tcaccagcgc agcggacaac
     1681 gaacgtgctg tggccgaagg tctcgaccgc ttcggccaga tcgacgtgtt catcgggaac
     1741 gccgggatct tcgactttgg tgccagcttc gtcgacacgc cgatcgatcg actggaaaag
     1801 ggcttcgacg agttgttcgc tctcaacgtc aaggggtacc tgctcggagt caaagccgca
     1861 gtaaacgccc tgatcgaaag tgatggctcg gttctgctca cagcgtccat gtcctcgtgg
     1921 cacgcgggag tggggggtgt ggtctacacg gcttcgaagc acgcggtggt cgggttggtc
     1981 cgccaactgg cccacgagct ggcgcccaag gtgcgggtca acggtgtggc gcctggcttc
     2041 atgcgcaccg acatccgtgg ccccaaggcg ctgggcctcg gtgatgtcac ttcctcctcg
     2101 cttcccgatc tggagcagat cgccgtcgcc accacacctc tccgattcct cccccggccc
     2161 gaggactaca ccggtcattt cgttcagctc gcctcacgca gcaacgcccc ggctaccacc
     2221 gggaccgttg tcgcatgcga tggcgggctc gacgtgcggg gaatcggact gccccaaccc
     2281 ggttgacacc cccgatcgcg gctaggtgag aggacctacg cttcgggcat tcccgcggac
     2341 ggagtcacga gatggatcac ccggcgaccc tcggtcgcag caccctgacg gtttcgcgcc
     2401 tcggagtcgg ggccatgacc tggggggagc ctcgg
//
LOCUS       DASTXO010000043         4717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_665541, whole genome shotgun sequence.
ACCESSION   DASTXO010000043 DASTXO010000000
VERSION     DASTXO010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665541"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /gene="leuD"
                     /locus_tag="VFU96_00835"
     CDS             <1..551
                     /gene="leuD"
                     /locus_tag="VFU96_00835"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEU5111826.1"
                     /translation="QIIPQQFLKRIERDGYGEFLFYNWAHTDDGKPSSDFILNDPARR
                     GARVLVTGPNFGCGSSREHAPWAIQQWGFEAVITASAADIFRNNSSYVGLLVVELAQD
                     AVSRLIELAADPKAVVTIDLESQTVTADGLEEQFDFNPTAKERLVTGLDGIGATMSHA
                     DVIAAHENVRPSWLPTTVDASS"
     gene            complement(557..2590)
                     /locus_tag="VFU96_00840"
     CDS             complement(557..2590)
                     /locus_tag="VFU96_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="HEU5111827.1"
                     /translation="MNDKSDPNPGDIAQIRDDIDRIDADILKLLAERKAHSLRIAKEK
                     GIHDRPSRDQSREEGLISDRIATGMEHDLDSGLVNRVWREIVNDSVRIQQEYLGRAER
                     PRQAMTVAIQGIEGSYSQLASQQYFDHKGMAVNYLRAPTFAAAIAAVQHEDADVAVLP
                     IENTTSGAISEVYDLLLESRLHFVGDVRFRIRHCLLGIEGASISELRRIYCHPQAVAQ
                     CSEFLAQLDHCEIVYFSDTALSGQKIHELGDPAIAAIASEEAARLFGLDVLKTGIANR
                     DENFTRFVVVAAEPAEVPDAVPSKTSIVMAVGNHPGALMDALAVFHDADINLVMLESR
                     PIANNPREELFYLDFDGNISEQAVQAVLETLMRQVRFLRVLGSYPSRDLRPKPVERKA
                     KKVRPQGRSALTQLSPSASSPKGYKLGSRGHKEQNTIVEVSGVRIGGDDLVMIAGPCA
                     VESWDQVMTTAKAVKESGGSILRGGCFKPRTSPYSFQGLAYEGLEMLVEAGKAYGLPV
                     VTEVVSPEDVEGVARKADILQIGTRNMQNFSLLSAAGRAHRPVMLKRGMSSSLDELLQ
                     AAEYILSEGNQQVFLCERGIRTFETSTRNTLDLSAVPVLRQRTHLPVIVDPSHAAGDR
                     DLVAPLALAAQAIGAHGIMVEIHPDPDNALSDGPQSLRFDQFQDLMLALGFED"
     gene            2787..2975
                     /locus_tag="VFU96_00845"
     CDS             2787..2975
                     /locus_tag="VFU96_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111828.1"
                     /translation="MRDLSKAFGIILGLAGIVWILQGLDVEFAPQSFMTGDPVWVVLG
                     AVAVLAGGAFLWRGARRK"
     gene            complement(2989..4095)
                     /locus_tag="VFU96_00850"
     CDS             complement(2989..4095)
                     /locus_tag="VFU96_00850"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU5111829.1"
                     /translation="MTLTAVMTASLSTGTALGISRADVDEACAESQEAYDIYRSARSD
                     FEVASVALDEANDELADAEYQERRIRTAYEARQEEKAELSTRVESQAVELYMQAASGP
                     SMGMVSLSSPSEALTAYEFLRANADESMQSVNDLSAVSSELDRLGVTLEAAVAELTTA
                     RDQQQEQTAEQESAMTSALGAYDDLSDRCRDLQAEYEAEQARLRAEAEERARQRAAAA
                     SSGGSGGSGGGSTGPIIGGIICPFSPGRTHFSNSWGAARSGGRSHKGTDMMAPFDEPI
                     YAVASGTVSTGSSGLGGRTIWLSTNVGSFYYAHLSGFAVGDGAHVSQGDLIAYNGNTG
                     NASGGAPHLHFEIHPGGRGSSAVNPYNAVASVCF"
     gene            4297..4452
                     /locus_tag="VFU96_00855"
     CDS             4297..4452
                     /locus_tag="VFU96_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111830.1"
                     /translation="MNPGDHEDYEDRLREKHQEKLWWEDWRGNKWLIFWLVVAVLAMI
                     VVGYLVS"
BASE COUNT          842 a         1484 c         1476 g          915 t
ORIGIN      
        1 atcagatcat tccccagcag ttcctgaagc ggatcgagcg agacggatac ggggagttcc
       61 tcttctacaa ctgggcgcac accgacgacg gaaaacccag ctcagacttc atcctcaacg
      121 atcctgcccg ccgcggggcg cgggtcctgg tcaccgggcc gaacttcgga tgcgggtcgt
      181 cacgtgagca cgctccatgg gccatccaac aatggggctt cgaggcggtc atcaccgcct
      241 ccgccgccga catcttccgc aacaactcga gctatgtcgg tctcctcgtt gtcgaactgg
      301 cccaagacgc ggtctcccgc ctcatcgagc tcgccgccga cccgaaggca gtggtcacca
      361 tcgatctcga gagccagacc gtcacggccg atggcctgga ggagcaattc gatttcaacc
      421 ccacggccaa ggagcgactg gtgacgggtc tggatgggat cggggccacc atgagtcacg
      481 ccgacgtcat cgctgcgcac gagaatgtcc gaccgtcgtg gttgcccacc acggtcgacg
      541 cctcctcctg aggctgtcag tcttcgaagc ccagcgccag catcaggtcc tggaactgat
      601 cgaaccgcaa cgactgaggt ccgtcgctga gggcgttgtc cgggtcggga tgtatctcca
      661 ccatgatccc gtgggcaccg atggcctgag ctgccaacgc cagtggcgcc accaggtccc
      721 gatccccggc agcatgtgat ggatcgacga tgacgggcag gtgggtccgc tgccgcagca
      781 cgggcaccgc actcaggtcg agcgtgtttc gcgtcgacgt ctcgaaagta cggatcccac
      841 gctcacagag gaacacctgc tggttcccct cggagaggat gtactcggcg gcctgcagga
      901 gctcgtccag ggaggagctc atccccctct tcaacatcac cgggcgatga gcccggcccg
      961 cagcggagag caaggagaag ttctgcatgt tcctggtccc gatctggagg atgtcggcct
     1021 tgcgggccac gccttcgaca tcctcgggcg acaccacttc ggtcaccacg ggcagcccgt
     1081 aagccttccc ggcctcgacc agcatctcca gaccctcgta ggccagaccc tgaaacgagt
     1141 agggagacgt cctgggcttg aagcagcctc cgcgcagaat cgagccgccg ctctccttga
     1201 ccgccttcgc agtcgtcatc acctggtccc aggactcgac ggcgcagggt ccggcaatca
     1261 tcaccaggtc gtctcccccg atcctcacgc cggagacttc gacgatcgtg ttctgctcct
     1321 tgtgaccccg gcttcccagc ttgtacccct tgggactgga agcgctaggc gaaagctgag
     1381 tgagggcaga tcgaccctgt ggcctcacct ttttcgcctt ccgctcgaca ggcttgggtc
     1441 ggaggtctcg cgacgggtag ctgcccagca cccgaaggaa gcggacctgc cgcatgagtg
     1501 tctccaagac ggcctgcacg gcctgctccg agatgttgcc gtcgaagtcg aggtagaaca
     1561 gctcctctcg tggattgttg gcgatgggcc gcgactcgag catgacgagg ttgatgtctg
     1621 cgtcgtggaa cacggcgagg gcgtccatca gcgctccggg gtggttgccc acagccatca
     1681 cgatggacgt cttcgagggg accgcatcgg gtacttcggc aggctcggcg gcgactacga
     1741 cgaaacgggt gaagttctcg tcccggttgg cgatcccggt cttgaggaca tcgagcccga
     1801 acaggcgcgc tgcctcttcg ctggcgatcg cggcaatggc tgggtctccc agttcgtgga
     1861 tcttctgacc ggagagcgcc gtgtcggaga aatagacgat ctcgcaatgg tcgagctggg
     1921 cgaggaactc agagcattga gccacggcct gaggatggca gtagatccgt cgcagctctg
     1981 agatcgaggc gccttcgatt ccgaggagac aatgacggat gcggaagcgg acgtcgccga
     2041 cgaaatggag cctgctctcc agcaacaggt cgtagacctc cgagatggcg cccgacgtgg
     2101 tgttctcgat cgggagcacg gcgacatcgg catcctcgtg ctgcacagcg gcaatcgctg
     2161 cagcgaaggt tggagccctc aagtagttga cggccatacc cttgtggtcg aaatactgct
     2221 gggatgcgag ctggctgtag gacccctcaa tcccctggat agccacggtc attgcctgac
     2281 ggggccgctc agcgcggccg aggtactcct gttggatcct caccgagtcg ttcacgatct
     2341 ctcgccaaac ccggttcacc aaacccgagt ccaaatcatg ctccatcccg gtcgcgatgc
     2401 gatcggagat gaggccctct tctctcgact ggtccctaga gggtcggtcg tggatgccct
     2461 tctccttggc gatgcgaagg ctgtgcgcct ttcgctcggc gaggagcttc aggatgtcgg
     2521 catcgatccg gtcgatgtcg tctcggatct gcgctatgtc acctgggttt ggatctgact
     2581 tgtcgttcat ttgatcgcct cggcgcgttg aaaggtggtg taggtacgga gtaacgcggg
     2641 ggtggggtga atcggcgcta ggcgccgaaa tagacgaacc cgaagaaacg ccgaggcgtg
     2701 gcgctcgtga tcgaggtcgg gcacgtcttc atgtcggcgg attatccgct gatgtcactg
     2761 tcgatgtcaa gagctatgtt cgagacgtgc gcgatctgag caaggcgttc ggcatcattc
     2821 tcggtctcgc tggcatcgtt tggatcctgc aaggtctcga cgtcgagttc gcaccgcaga
     2881 gtttcatgac cggcgaccct gtgtgggtcg tcctgggcgc cgtcgcggtc ctcgccggcg
     2941 gagcattcct ctggagaggg gcgcggagga aatagggatt ctgccggctc agaagcagac
     3001 cgaggcgacc gcgttatagg ggttgaccgc gctcgagcct cggccgccgg ggtggatctc
     3061 gaagtgaagg tgcggggcac cgcccgacgc gttacccgtg ttgccgttgt atgcgatcag
     3121 atcgccttga ctcacgtgcg ccccgtctcc gaccgcaaac ccgctcaagt gggcgtagta
     3181 gaacgacccg acattcgtcg agagccatat cgtcctcccg ccgaggccac tgctccccgt
     3241 gctgaccgtc ccgcttgcca ccgcatagat cggctcgtcg aacggggcca tcatgtcggt
     3301 tcccttatgg gatctgcccc ccgaacgggc cgcgccccac gaattgctga agtgggtccg
     3361 gcctgggctg aaggggcaga tgatgccgcc gatgatcgga ccggtggaac cacctccgga
     3421 accacccgat ccgccgctcg aagccgccgc cgcacgctga cgcgccctct cctcggcttc
     3481 ggcgcggagg cgggcctgct cagcttcgta ttcggcctgc aggtccctgc acctgtcgct
     3541 cagatcgtca tacgccccaa gcgccgacgt catcgctgat tcttgttccg ctgtctgctc
     3601 ctgttgctga tcgcgtgccg tggtcagctc tgcgacggct gcctcaagcg tgactcccaa
     3661 tcgatccagt tcggaggaga ccgcggacag atcgttcacg gactgcatgc tctcatcggc
     3721 gttcgcccga aggaactcat aggcggttag tgcttcgctt ggcgatgaca ggctgaccat
     3781 ccccatggat ggcccggaag ccgcctgcat gtacagctcc acggcctgtg actcgacccg
     3841 ggtcgacaac tctgcctttt cctcttgacg tgcctcatac gcggtgcgta tccggcgttc
     3901 ctggtattcg gcgtcggcga gctcgtcgtt ggcctcgtcg agtgcgaccg acgccacttc
     3961 gaagtcggaa cgggccgagc ggtagatgtc gtaggcctcc tgggactcgg cacatgcctc
     4021 atcgacatcg gcacgggata tacccaatgc cgttccagta gaaagacttg cggtcatgac
     4081 cgccgtcaga gtcatgacaa cgaggacggt ccgtctcgat ttggaacgtt ttgggttcgc
     4141 tttgtctgtt cggagcatgc tcgctggtct ctcgtcgacc tgagccgcgc caggcgcgac
     4201 aacagttcgc ttctacaaag gtggtgaatg ggacaggatg gcacggattc accagctagc
     4261 tggacttcat cgaagccctg tgtagggtcg gatgccatga accccggcga tcacgaggat
     4321 tacgaggacc gccttcggga gaaacaccag gagaaactgt ggtgggagga ctggcgcggg
     4381 aacaagtggc tgatcttctg gctcgtcgtg gcggtgctgg cgatgatcgt tgtggggtac
     4441 ctcgtcagtt gaccccaact cggccctagc cgggcgcgcc gaccccccgg gcgatggtgg
     4501 cacccggagg gcgacttcgt tggagaggcc ctctccgctt cccctgtttc cgctccaacg
     4561 ccttccccat atcggcaaaa cgacgtctgg aacttgagag gggccgggac atataagaag
     4621 ccccaacaag tctgaaccga agttccgacc cgtcggggct tcagagcgga actataccga
     4681 atctgatcga ggcgtcaaat cggatgcatc cctgtcg
//
LOCUS       DASTXO010000044         2088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_628652, whole genome shotgun sequence.
ACCESSION   DASTXO010000044 DASTXO010000000
VERSION     DASTXO010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628652"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VFU96_00860"
     CDS             <1..602
                     /locus_tag="VFU96_00860"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU5111831.1"
                     /translation="GVTGPAYDAPMDPSDQPPSPGLISSQPLYKGRVVDLSLDHVLLP
                     NGNETDLELIRHPGAAVVLPVETGADGVAHALLVRQYRYAAGGWLLEVPGGKLDGPED
                     PELCARRELEEEIGHEAGELIPLGSILTTPGFTDERIWLYLGRDLKPTGGGPALEADE
                     VLEVERIPLSEAVARAIDGDIIDAKTVAALLRAAAMLGI"
     gene            complement(615..773)
                     /locus_tag="VFU96_00865"
     CDS             complement(615..773)
                     /locus_tag="VFU96_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111832.1"
                     /translation="MSDEYLPEADGPEEAPDEVRAPFSFKLMIALTIAYLGWRLVQGI
                     IWVIEKLS"
     gene            complement(770..>2088)
                     /gene="der"
                     /locus_tag="VFU96_00870"
     CDS             complement(770..>2088)
                     /gene="der"
                     /locus_tag="VFU96_00870"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEU5111833.1"
                     /translation="LMSRLPVVVVVGRPNVGKSTLVNRIIGSRSAVVEELSGVTRDRR
                     EFDGDWSGHEFVLVDTGGWEMKPSDHMGASIKEKAEAAVSGADVLIFVVDGTSELTDD
                     DTGVISVLRSAQIPVVLAANKIDNETMEGNIDRLWGLGLGEPYPISAYHGRGVGELLD
                     QVIGALPETADAEHRDEIPRVAIVGRPNVGKSTLLNKLVGERRVIVSETPGTTRDPID
                     VDVEMDGTRYRLIDTAGIRRKPQITEDADFYAVLRAREALESADVALLLIDAMEGVTH
                     QDQRIASEVVQAGVALVIILNKWDALDDDQRELTERSLPDRFGFVGWAPVLRMSAQTG
                     ARIQRLPGAIEAVLEGRATRLPTGQLNRAVRKWAAAHPPPVRKGRRPKIHYVVQAGTA
                     PPTFVVFISGGTLGDDYLRFLENRLRDEADFTGTPLHIVSRAGERR"
BASE COUNT          368 a          680 c          651 g          389 t
ORIGIN      
        1 cgggcgtgac cggccccgct tacgatgccc cgatggatcc gtccgatcag ccaccgtccc
       61 ctggattgat ctcgagccag cccttgtaca agggtcgagt tgtcgatctc tccctcgacc
      121 acgtgctgct tcccaacggg aacgagaccg atctcgagtt gatccgccac ccgggagcag
      181 ccgtggttct tcctgtggag accggcgcgg atggagtcgc ccacgctctt ctcgtccgcc
      241 agtatcggta tgccgccggt ggctggctgc tggaggtccc cgggggcaag ctggacgggc
      301 cagaggatcc cgagttgtgc gcccggcgcg agctcgaaga ggaaatcggg catgaggccg
      361 gcgaactgat cccactcggc tcgatcctga ccacgccggg attcaccgac gagcggatct
      421 ggctctatct agggagagat ctgaagccga ccggtggcgg cccggcattg gaggccgatg
      481 aggttctgga agtagagcgc ataccgctgt ccgaggccgt ggcgcgagcg atcgacggcg
      541 acatcatcga cgccaagact gtggcggcac tgctcagggc ggctgcgatg ctcggaatat
      601 gagatcgatc tcgatcagct cagcttttcg atcacccaga tgattccctg gaccaatctc
      661 caaccgaggt aggcaatcgt cagagcgatc atcagcttga agctgaatgg cgcccgaacc
      721 tcatctggcg cctcttcggg cccatcggcc tcggggaggt actcgtcgct caccgacgct
      781 ccccggcccg agacacgatg tgcaacgggg tcccggtgaa atcggcctcg tctctgagcc
      841 ggttttccag aaagcgaagg tagtcatcgc cgagcgtccc gccggagatg aagaccacaa
      901 aggttggtgg agcggtcccg gcctgaacca cgtagtggat cttgggacgg cggccttttc
      961 ggacgggcgg cgggtgggcc gcagcccatt tccgaaccgc ccgattcagc tgaccagtgg
     1021 gaaggcgcgt ggcccggccc tccaagaccg cttcgatggc ccccggtagc cgctgaatcc
     1081 gagcaccggt ctgtgccgac atgcgaagga caggcgccca accgacgaac ccgaagcgat
     1141 ctggcaacga gcgctcggtg agctctcttt ggtcatcgtc gagagcatcc cacttgttga
     1201 ggatgatcac cagggcgact ccggcctgga cgacttcgct cgcgatgcgc tggtcctgat
     1261 gagtgactcc ctccatggcg tcgatcagaa gcagggctac gtctgcactc tccagcgctt
     1321 cgcgggcacg gagcacggca tagaagtcgg catcctcggt gatctgtggc ttgcggcgta
     1381 tccccgctgt gtcgatcagg cggtatcgag tcccgtccat ctcgacgtcg acgtcgattg
     1441 ggtcacgagt tgtccccggt gtctcggaga cgatgacccg gcgctcgccg accagcttgt
     1501 tcagcagcgt cgactttccg acgttgggcc ggccgacaat ggccacgcga ggtatctcgt
     1561 ctcgatgctc cgcgtcggcc gtctcgggca gcgcgccgat aacctggtcc aggagctctc
     1621 ccactcccct tccgtggtat gcgctgatcg gatagggctc cccgagcccc aggccccaga
     1681 gtcggtcgat gttgccctcc atcgtctcgt tgtcgatctt gttggcggcc agcaccaccg
     1741 ggatctgtgc cgacctgagc acggagatga cgccagtgtc gtcatcggtg agctcgctgg
     1801 tgccatcgac cacgaagatc aagacatccg cgcccgatac cgcggcctcc gccttctcct
     1861 tgatcgaggc acccatgtga tcggacggct tcatctccca tccgccggtg tcgaccagga
     1921 cgaattcatg gccggaccaa tctccgtcga actcgcgacg gtcgcgggtg acacctgata
     1981 gttcctcgac caccgcagac ctcgaaccga tgattcgatt gaccagggtc gacttaccca
     2041 cgttggggcg acccacgact acgacgacgg gaagtctgct catcaagg
//
LOCUS       DASTXO010000045         7806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_370949, whole genome shotgun sequence.
ACCESSION   DASTXO010000045 DASTXO010000000
VERSION     DASTXO010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7806
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370949"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VFU96_00875"
     CDS             complement(<1..346)
                     /locus_tag="VFU96_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111834.1"
                     /translation="MTVEAPVFDETAVEVEPARGPMDWVRKNLFNTWYNVVLTLALSA
                     LLVWAVVGLARGLLGADFEIIRVNLRLFMIGDFPGDQLWRPWTATYLITTLIGLVAGS
                     VAAGARDRANEAG"
     gene            complement(343..1509)
                     /locus_tag="VFU96_00880"
     CDS             complement(343..1509)
                     /locus_tag="VFU96_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5111835.1"
                     /translation="MVEEVRSHTPLWRDVRVLRLVAQVAAAAIVAFIAYVLWFNLTNN
                     LRAAGLPTGFDFLTQPLGVDIPGADLSSGAPIWRGLLVGIKNTLALVVVGIPLLTIIG
                     VMVGVARLSTNWLVAKAAGLYVEILRNIPPLLIIYFVFNAVVLQFPLLQESLNLGDFL
                     IVNNRYIAVVGFTSEPDFGPFLLIMAGALVIAAGVWWWRTRRSERTGVHHHRFLWSLA
                     VFSVISIVGFLVLGGPFGLSRPVLDGRALEGGYQGLAAYWAVLAALVLYTASHVAEIV
                     RGSILAVPKGQSEAANAIALTPFQRLRYVVMPQALRIAIPPIISQYLNYTKNTSLAIA
                     IGYAEVTRLTFQMIGNGQPAPQMIAILMGIYLGFSLVISLIVNVVNRRMQLVTR"
     gene            complement(1593..2765)
                     /locus_tag="VFU96_00885"
     CDS             complement(1593..2765)
                     /locus_tag="VFU96_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5111836.1"
                     /translation="MKASRLFAASTALMLVLAACTQAADTTTTSSGETTETTEAAGEE
                     TTTSAAGEETTTTAGEEAPAGEGKLAEIQARGSLLCGVNNAVPGFGFVDETGEHVGFD
                     IDFCRAIAAGVLGDAEAVTYVDLTAEQRFTALQSGEVDVLVRNTTFTSSRDGGNAATF
                     LTTTYYDGQGMMVPADSGFTTIDEMTDTTICVLSGTTTELNLTTRMAGINYTPLTFEE
                     NPLIQAAFIEGQCDGWTSDKSQLAGIQSAWPEDQGGPEALVILEEVFSKEPLGPAVLD
                     GDSQWAQAVDWIVLSTIQAEEFGIDSTNVDTFADTEDANIRRFLGLDVETDEGTAPFD
                     PGLGLPVDYAAQVIGQVGNYAEIFDRNVGEGSPLGLPRQYNALWTDGGLLYSPPYR"
     gene            2893..4125
                     /locus_tag="VFU96_00890"
     CDS             2893..4125
                     /locus_tag="VFU96_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111837.1"
                     /translation="MSERIVARIVWVVAALALVATGFGIWDDVRNAVGDESFFFILDG
                     VAWALIPAVFILTGALIVSRQPQNVIGLLLMIPGLVMLAAGVTDLAFGPLVEQPASLS
                     PGLWLRLWFDNWSWVLLIFPIFHLMLVFPTGRLLSARWRWVVGLEIVMVLVMMLGTAF
                     GDMIGPLNVPEEQAWSVPNPIGFISESSFWPKFEAFWTAGLLVMTVLSLLAMVVRFRV
                     ASAAERQQLKWLLFAVTVFALNYGITAVTAGELETGGLGDFLFLISVMGIPISIAIAV
                     LRFHLYDLDLIIRRTLLYAVLTGLLVGTFALSVFVIQRVIGGFVGEDSALGIATSTLL
                     IAALFNPLRRRLQGLIDRRLYRSKYDAQRVIDQFVATARNEPDMNLLTIDLLDVVDQT
                     VQPAVQGLWIKGAESPGT"
     gene            complement(4130..4870)
                     /locus_tag="VFU96_00895"
     CDS             complement(4130..4870)
                     /locus_tag="VFU96_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017592941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU5111838.1"
                     /translation="MSPLLEVRGLWKQFGDLTVLAEVSLCLSTHEVLAVVGPSGSGKS
                     TLLRCINLLEPVDDGEILLDGVDIADPEFDADAARRRIGIVFQSFNLFPHLTVLDNIT
                     LAPNRVLKVTKEEAQITAQHLLTRFDLADKRDEYPDRLSGGQQQRVAIIRALAMSPSL
                     MLFDEPTSALDPELVGEVLDAIKRLAAEGMTMIVVSHELAFARDVANRVLFMDEGRVV
                     EEGSPDQVLVSPTTDRARRFLRLIAETT"
     gene            complement(4867..5595)
                     /locus_tag="VFU96_00900"
     CDS             complement(4867..5595)
                     /locus_tag="VFU96_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU5111839.1"
                     /translation="MLATQAPGWDRFKAYFFDWDIYRDSLPGIAEAFLINVKIFLIAE
                     AIILVTALGLAILRGLPGPVFFPIRALAIVYADLFRGIPTILVILMLGVGMPSLQIEG
                     IPESQVFWAIVSLVLVYTAYVSEVYRAGIESVHPSQDAAARSLGLTRFQSLRFVVVPQ
                     AVRRVIPPLLNDFVALQKDSALLALLGVQEAFRRSQIYVAETFNFTAYLAAALLFMLV
                     TVPLARFTDHLIRRQRERTAGTSA"
     gene            complement(5731..6615)
                     /locus_tag="VFU96_00905"
     CDS             complement(5731..6615)
                     /locus_tag="VFU96_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006383175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5111840.1"
                     /translation="MRKALVVLGIPVVLTALALALAATATGSPSAAAQVPGCAKGSLN
                     LENEGRLTLATDNPAFPPWWGGGETRKPWKISNPYSGQGYESAVAYAVARMLGFRKAQ
                     VQWEHVPFAQSYRPGDKNFDFYMAQVSYSPQRDSAVDFSNAYYFVNQSVVGRKGTDIA
                     NAKSVAALKPYRLGAQLGTTSYQYIVNNIRPSSRPLAYRTNDLAVQALKNGQIDGIVV
                     DLPTAFYVTAVQVEDGVIVGQLPSRGAREHFGMVFEQGNPLRRCVNKALNRLWANGTI
                     KSIQRTWLAKAGGAPVLG"
     gene            6695..7723
                     /gene="ligD"
                     /locus_tag="VFU96_00910"
     CDS             6695..7723
                     /gene="ligD"
                     /locus_tag="VFU96_00910"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003958639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEU5111841.1"
                     /translation="MASPFVELEVGERIVKLTNPDKVLFPGAGKTKRDLAEYYISVGD
                     GIVRTLYERPTQLRRFPDGITGEAIYQKRVPEKRPEWVETARVTFPSGRHADELCVTE
                     LAQVVWAANLAVVDFHPWPSRRKDTEHPDQLRIDIDPQPGTTFKDGKRVAALVRDVLD
                     EIGYVGWPKTSGNRGIHVFCRIEPLWEFPVVRRAALAFARQVERRLPKKVTTAWWKEE
                     RGRRVFIDFNQNARDRTMASAYSVRARKDATVSAPVTWDELPDVETEDFTMSTMPERF
                     AKLGDIQAGMDDAVCDLGVLLEWVEREEKEGVGEAPYPPNFPKMPGEPRRVQPSRARK
                     PEGDVKPA"
BASE COUNT         1388 a         2488 c         2593 g         1337 t
ORIGIN      
        1 gacccgcttc gttggcccgg tcacgggctc cggcggccac ggaaccggcg acgagcccga
       61 tcagcgtggt gatgaggtag gtggccgtcc agggacgcca caactgatcg cccgggaagt
      121 caccgatcat gaagagcctc agattgaccc ggatgatctc gaaatcggcc ccgagcagcc
      181 cacgggcaag cccgaccacc gcccaaacca gaagggcgga cagagccagg gtgagcacga
      241 cgttgtacca ggtgttgaac aggttcttcc gtacccaatc cattggcccc cgcgccggct
      301 ccacctcgac ggcggtctcg tcgaacactg gtgcctcgac cgtcatcggg tcaccagctg
      361 catgcggcga ttgaccacat tcacgatcaa cgagatgacc agcgagaacc cgagatagat
      421 ccccatgagg attgcgatca tttgcggagc cggttgcccg ttgccgatca tctggaacgt
      481 gagtcgggtg acctcggcgt acccaatcgc gatggccaaa gacgtgttct tggtgtaatt
      541 caggtactga ctgatgatcg gtgggatggc gatccgcagc gcctggggca tgacgacata
      601 acgaagacgc tggaatgggg tgagggcgat ggcattcgcc gcctcggact ggcccttggg
      661 cacagcgagg atgctccccc ggacgatctc ggcgacatga gacgccgtat acagcacgag
      721 cgcggcgagg accgcccagt acgccgccaa cccctggtat ccgccctcga gggcccgccc
      781 gtcgagcacc gggcgggaca gaccgaacgg gccgcccagg acgaggaagc cgacgatcga
      841 gatcaccgag aagacggcga gtgaccatag aaagcggtgg tgatgcacac cggtccgctc
      901 cgagcgccgg gttctccacc accacacgcc ggcagcgatg accaaagcac cggccatgat
      961 cagcaggaat ggcccgaaat cgggctcgga agtgaatccg actaccgcga tgtagcggtt
     1021 gttcacgatg aggaagtcgc cgaggttcag cgactcctgg agcagtggga actgcaggac
     1081 aacggcattg aacacgaaat agatgatgag gagcgggggt atgttgcgca ggatctccac
     1141 atagagcccg gccgctttcg ctaccagcca gttggtggag agacgggcca ctccgaccat
     1201 gacaccgatg atggtgagca gcgggatccc caccaccacc agtgccagcg tgttcttgat
     1261 gccgacgagg agcccccgcc agatcggggc acctgagctc aggtcggcgc cggggatgtc
     1321 gaccccgagt ggctgggtca ggaaatcgaa gccggtcggg agcccggcag cgcgcaggtt
     1381 gttggtgagg ttgaaccaga ggacataggc aatgaacgcg acgatggcag cggccgccac
     1441 ctgggctacc agacgcagga cccgcacatc cctccacaga ggagtgtgcg accgaacctc
     1501 ctccaccacg gtcaagcgat caccacctgt cgatcaccct tctggctcgg agatccgacc
     1561 ccatcggggt cggatctcac ggccagattc actcagcggt aaggcgggga gtagagcaac
     1621 ccaccatcgg tccacagggc gttgtactgc cggggaagac ccaacgggct tccctcgccg
     1681 acgttgcgat cgaagatctc ggcgtagttg cctacctgtc cgatcacctg agcggcgtag
     1741 tccaccggga gcccgagccc ggggtcgaac ggagccgtcc cctcgtcggt ctcaacgtcc
     1801 agcccgagga atcgcctgat gttggcgtcc tcggtgtcgg cgaacgtatc gacattggtc
     1861 gagtcgattc cgaactcctc ggcctggatg gtggacagga cgatccagtc cactgcctgc
     1921 gcccattgcg agtcgccgtc gagaacggcc gggcctagtg gctctttgga gaacacctct
     1981 tcgaggatca ccaacgcctc ggggccacct tgatcctccg gccaggccga ctggatcccg
     2041 gcgagctggg acttgtccga cgtccaacca tcacactggc cctcgatgaa ggccgcctgg
     2101 atcagcgggt tctcctcgaa ggtcagcggc gtgtaattga tcccggccat ccgagtggtc
     2161 aggttcaact ctgtggttgt ccccgagagg acgcagatcg tggtgtcggt catctcgtcg
     2221 atcgtggtga acccactatc ggccgggacc atcatcccct gcccgtcgta gtacgtcgtg
     2281 gtgaggaagg tggcggcatt gccgccgtca cggctcgagg tgaaggtggt gttccggacc
     2341 aagacgtcga cctcacctga ctggagggcg gtgaatctct gctctgcagt caggtccacg
     2401 taggtcactg cctcggcgtc accgaggaca ccggcagcga tggcgcggca gaagtcgatg
     2461 tcgaagccca cgtgctcgcc ggtctcgtcg acgaacccga acccgggcac cgcgttgttc
     2521 acaccacaga gcaaactgcc ccgggcttgg atctcagcca gcttgccttc accggccgga
     2581 gcctcctcgc cggccgtggt ggtcgtctcc tcaccggcag cgctcgtcgt cgtctcctca
     2641 ccggcagctt ccgtcgtctc cgtcgtctcg ccgctggacg tcgtggtcgt atccgctgcc
     2701 tgggtacatg cagccaggac cagcatcagg gcggtcgagg cggcaaatag cctgcttgcc
     2761 ttcataacta cctccgttgg atcgctttgt gggcgtcacg aggccacgta gcgcgccgga
     2821 atcgacccta gccaccgagc catggtgacg ggtctcgatc ggtaacctga cttcactttc
     2881 cgaaaggcgt ccgtgtcgga acgaatcgtt gcccggatcg tgtgggtcgt agctgccctg
     2941 gccctcgtcg ctaccgggtt cgggatctgg gacgacgtgc gaaatgcggt tggcgacgaa
     3001 tcatttttct tcatcctgga cggcgtcgcc tgggcgctga taccagcggt gttcatcctg
     3061 accggggcgc tgatcgtgtc ccgacagccc cagaacgtca tcgggttgtt gttgatgatc
     3121 cccggtctgg tcatgctggc cgcgggggtg accgacctcg cttttggtcc actcgtggag
     3181 cagccggcgt cgttgtctcc ggggctctgg ctccgactct ggttcgacaa ctggtcgtgg
     3241 gtgctgttga tctttcccat cttccacctg atgctggtct tccccaccgg caggctgctc
     3301 tccgcacgtt ggcgatgggt cgtcgggctg gagatcgtca tggtgctcgt gatgatgctc
     3361 ggcacggcgt tcggagacat gatcgggccg ctcaatgtgc cggaggagca ggcctggtcc
     3421 gtgcccaacc cgatcggatt catctccgag agctccttct ggccgaaatt cgaggccttt
     3481 tggaccgccg gccttctcgt catgactgtg ttgtctctat tggcgatggt cgtgcgcttc
     3541 cgggtcgcct ccgccgcgga gcgccaacag ctcaaatggt tgctcttcgc cgtcaccgtg
     3601 ttcgctttga actacgggat caccgccgtc accgccgggg aactcgagac cggtggtttg
     3661 ggcgacttcc ttttcctgat ctcagtgatg ggcatcccga tatccatcgc gatcgccgtc
     3721 cttcgcttcc acctgtacga cctcgacctg atcatccggc ggacgctgct ctacgccgtg
     3781 ctcaccggtc tgttggtcgg gaccttcgcc ttgtcggtct tcgtgatcca gcgggtgatc
     3841 ggaggattcg tcggagagga ctccgctctc ggcattgcca cctccacgtt gctcatcgcc
     3901 gcgttgttca acccgctgcg ccgccgtctc cagggcctca tcgatcgccg cctctaccgg
     3961 agcaagtacg acgcccagag agtcatcgac cagttcgtgg ccaccgccag aaacgagccc
     4021 gacatgaacc tgctcacgat cgatttgctc gatgtcgtcg accagaccgt ccagcccgcg
     4081 gttcaggggc tctggatcaa aggagccgag agcccgggca cctgaccgat caagtggtct
     4141 cggcgatcaa acggaggaac cgccttgccc tgtcggtggt cgggctgacc agcacctgat
     4201 ccggagagcc ttcctcgacg acccggccct catccatgaa caacacgcgg ttcgccacgt
     4261 cccgggcaaa ggcgagctca tgggacacga cgatcatcgt catcccctca gcggccagcc
     4321 tcttgatcgc atcgagcacc tcgccgacca gctccgggtc cagcgccgat gtcggctcgt
     4381 cgaaaagcat caacgagggg ctcatggcca gggcccggat gatcgccact ctctgttgct
     4441 gtccgccgga gagccggtcc gggtattcat cacgtttgtc ggcaaggtcg aagcgggtga
     4501 ggagatgctg agcggtgatc tgggcctctt ccttggtgac cttcagcact ctgttggggg
     4561 ccagagtgat gttgtcgagg accgtcaagt gtggaaacaa gttgaacgac tggaacacga
     4621 tcccgatacg ccgacgagcg gcatcggcgt cgaactcggg gtccgcgatg tcgacaccgt
     4681 cgagcaggat ctcgccatcg tcgaccggct cgagaaggtt gatgcatcgg aggagggtcg
     4741 acttgcccga ccccgagggc cccaccaccg ccaggacttc gtgggtggac aggcagaggc
     4801 tgacctcggc gaggacagtc aggtcaccga actgcttcca gagaccgcga acctcgagaa
     4861 gtgggctcac gccgaggtcc cggcggtccg ctcccgctga cgccggatca gatggtcggt
     4921 gaaccgggcc aggggcacag tcacgagcat gaagaggagg gctgcagcca gatacgcggt
     4981 gaagttgaag gtctcggcca catagatctg tgatcgacgg aacgcctcct gcactccgag
     5041 cagcgcgagc agggccgagt ccttctggag ggcgacgaaa tcgttgagga gcggggggat
     5101 gacacgccgc actgcctgcg ggacgacgac gaagcgcagg gactggaagc gggtcagccc
     5161 gagggagcgc gccgccgcgt cctggctcgg atgcaccgac tcgatcccgg cgcgatagac
     5221 ctcggagacg taggcggtgt acacgaggac gagcgagacg atcgcccaga agacctgtga
     5281 ctccgggatg ccttcgatct gcagcgaggg catgccgacg ccgagcatga ggatgacgag
     5341 gatggtcggg atcccgcgga agaggtctgc atagacgatc gcgagcgccc ggatagggaa
     5401 gaagaccggt cccggaagcc cgcgcaggat ggccagcccg agggcggtca cgaggatgat
     5461 cgcctcggcg atgaggaaga tcttcacgtt gatgaggaac gcctcggcga tccccgggag
     5521 cgagtcccgg tagatgtccc agtcgaagaa gtacgccttg aaccggtccc agcccggtgc
     5581 ctgcgtggcg agcaccgcga gcgcggcgaa gaagacgagc gtgctcgcga ccgcgatcag
     5641 gaccccgcgc cccggcttgc gtgctcctcc ggggcggggg gacccgatgc tcaggatccg
     5701 tgagcggcgg tcgccttcca gcgctgcgcg ctatccgagg acgggcgcgc cgcccgcctt
     5761 cgcgagccag gtgcgctgga tgctcttgat cgtgccgttc gcccacaggc ggttcagtgc
     5821 cttgttgaca cagcgccgca acgggttgcc ctgctcgaag accatgccga agtgctcgcg
     5881 ggcgcctcgg ctcgggagct gcccgacgat gacgccgtcc tcgacctgaa cggcggtcac
     5941 gtagaacgcg gtcggcaggt cgacgacgat gccgtcgatc tggccgttct tgagcgcctg
     6001 caccgcgagg tcgttcgtcc ggtatgcgag cggccgcgag ctcgggcgga tgttgttgac
     6061 gatgtactgg tagctcgtcg tgccgagctg ggcaccgagc cggtacggct tgagcgccgc
     6121 gaccgatttc gcgttcgcga tgtcggttcc cttgcggccg acgacggact ggttcacgaa
     6181 gtagtaagcg ttggagaagt ccacggcgct gtcgcgctgc ggcgagtagg agacctgcgc
     6241 catgtagaag tcgaagttct tgtcgccagg tcgatacgac tgtgcgaacg ggacgtgctc
     6301 ccactgcacc tgcgccttgc ggaagccgag catgcgcgcg acggcgtacg cgacggcgga
     6361 ctcgtagccc tggccgctgt acgggttcga gatcttccac ggcttgcgcg tctcgccgcc
     6421 gccccaccag ggcgggaacg ccgggttgtc cgtcgcgagc gtcaggcgtc cctcgttctc
     6481 caggttcagg ctgcccttcg cgcagccggg gacctgggcc gcggcggagg ggctgcccgt
     6541 ggccgtggcc gcgagcgcca gggcgagtgc cgtgaggacg acggggatgc cgagaacgac
     6601 gagagccttg cgcatcgcag atctactcct aacgccaggg aggacggggc aacgagcggg
     6661 ccgagcgtac caccgggccg ctagcgtgcg gcgcatggcc tcgccgttcg tcgagctcga
     6721 agtcggtgag cggatcgtga agctcacgaa cccggacaag gtgctcttcc caggggccgg
     6781 caagacgaag cgcgacctgg ccgagtacta catctcggtc ggggacggca tcgtgcgcac
     6841 gctgtacgag cggccgacgc agctccggcg cttcccggac ggcatcaccg gcgaggcgat
     6901 ctaccagaag cgcgtgcccg agaagcgtcc cgaatgggtc gagacggcgc gggtcacgtt
     6961 cccgtccggg cgtcacgccg acgagctctg cgtgacggag ctcgcgcagg tcgtctgggc
     7021 ggcgaacctg gcagtcgtcg acttccaccc atggccgtcc cgccgcaagg acaccgagca
     7081 tcccgaccag ctccgaatcg acatcgaccc gcagcccggg acgacgttca aggacgggaa
     7141 gcgcgttgct gcgctcgtcc gggacgtgct ggacgagatc ggctacgtcg gctggccgaa
     7201 gacgtcgggc aaccgcggta tacacgtctt ctgccgcatc gagccgctct gggagtttcc
     7261 ggtcgtgcgt cgcgcggcgc tcgcgttcgc gcggcaggtg gagcggcgcc tgccgaagaa
     7321 ggtcacgacc gcctggtgga aggaggagcg cggccgtcgc gtcttcatcg acttcaacca
     7381 gaacgcgcgc gaccgcacga tggcttccgc gtactcggtg cgagcgcgga aggacgcgac
     7441 cgtgtcggcc ccggtcacct gggacgagct tccggacgtc gagaccgagg acttcacgat
     7501 gtcgacgatg cccgagcgct tcgcgaagct cggcgacatc caggcgggca tggacgacgc
     7561 cgtctgcgac ctgggcgttc tcctcgagtg ggtcgagcgc gaggagaagg agggggtcgg
     7621 cgaggcgccc tatccgccga acttcccgaa gatgccgggc gagccacggc gcgtccagcc
     7681 ttcccgggca cgcaagcccg aaggcgatgt aaagcctgct tgacatagag tcgaagtcaa
     7741 gctagcttta catctcgatg cgaaacgaca tccgggcgct gcggaccgaa cgagggttgt
     7801 cgcagc
//
LOCUS       DASTXO010000046         2173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_923681, whole genome shotgun sequence.
ACCESSION   DASTXO010000046 DASTXO010000000
VERSION     DASTXO010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923681"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1361)
                     /locus_tag="VFU96_00915"
     CDS             complement(<1..1361)
                     /locus_tag="VFU96_00915"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEU5111842.1"
                     /translation="MAKAIQPLIELLGVTKAYPGVIALQDLDLAIIPGRVHGVVGLNG
                     AGKSTLVKVLAGTISPDSGSISARERPGDGRLVNMVPQDIVVVPQLSIGRNILLGREP
                     GFPVRRRLNSREERFVRSALGRVGLDIDPESLPSACSPQELRLVQIAKAMADPADVLL
                     LDEPTAVLAEGDASRLLKTLSTLRDAGEAIVYISHRLGEVLQIADEITVLRDGRKVAS
                     FTTGEVGRKDLLDLLTKHGTSGNTGTFEAPERGGVVLEVEGLVGLGFRDVNLQVRAGE
                     IIALVGVQGAGQSAVVQALAGLRPTASGSVRLGGRSVTISSPSASTRAGLMLVPADRR
                     ARGVVAAMDTVENIALSPRSSAKRGGFRKRRAERDVASRYVNRFGIKTPRLATHLATL
                     SGGNQQKVVLSRAIEARPEVLLLDEPTQGIDVATKWEILARIKSEAREIGFPVLAASS
                     ELE"
     gene            complement(1381..>2173)
                     /locus_tag="VFU96_00920"
     CDS             complement(1381..>2173)
                     /locus_tag="VFU96_00920"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEU5111843.1"
                     /translation="AGEEAGVEVTTLAAEGYASPDLQLSQVEDAIAGGTDAIVMAPSD
                     IQGSVPAVDLAVDADIPVVNISTEVAQGDVYMVMQDDYVLGQMMADRLAEVVGEEGGQ
                     GVLIAGPANATWSRKRTVGFSDQVAAEYPNIEIVAAPTQLVDPAEGLASFEDAVQAFP
                     EIDWIASVHYFILQPSAIPAEYQGEIPYVSFGYEPDSIAALEAGTLDSVFAIEPVSMG
                     RVGIGTAISLLNGEEQPQVNCLPAPLFTVDEIGTPVADNELIDEG"
BASE COUNT          395 a          717 c          665 g          396 t
ORIGIN      
        1 tcctctagtt cggaggaagc cgccaaaacc gggaagccaa tctccctggc ctcgctcttg
       61 attcgggcga ggatttccca tttggtagca acgtcgatcc cttgggtcgg ctcgtccaag
      121 agcaggacct cagggcgggc ctcgatcgca cgactcagca ccactttctg ctggttgcct
      181 ccgctcaacg tggccaaatg tgtggccagc ctgggggtct tgattccgaa ccggttcacg
      241 tagcggctgg ccacgtcacg ctccgcccga cgcttgcgga acccaccccg cttcgccgaa
      301 gaacgggggc tgagagcgat gttctccacg gtgtccatcg ccgcgacgac tccccgggcg
      361 cggcgatccg caggtaccag catcagcccc gccctggtgg atgcggacgg actcgaaatc
      421 gtcacgcttc gaccgccaag ccggactgac cccgaggctg tgggacggag gccggcgaga
      481 gcctgcacca ctgcgctctg acctgcaccc tgcactccga cgagcgcaat tatctcaccg
      541 gccctgacct gcaggttgac atcacgaaac ccaagtccga cgaggccctc aacttccagg
      601 accacccctc ctcgctcggg ggcttcaaag gtgccggtgt tgcccgaggt tccgtgcttg
      661 gtgagcaggt cgagcaaatc cttgcgcccg acctcgcccg tggtgaacga agccactttg
      721 cggccgtcac ggaggacggt gatctcgtcc gcgatctgga ggacttcgcc gagtcggtgg
      781 ctgatgtaga cgatcgcctc gccggcatca cgaagcgtgg agagcgtttt caggagccgg
      841 ctggcgtcgc cttccgctag gactgcagtc ggttcgtcga ggagaagcac gtctgctgga
      901 tccgccatcg ccttggcgat ctggacgaga cgcaactcct ggggggagca cgccgacggg
      961 agtgattccg ggtcgatatc aagcccgaca cgacccagcg ccgaccggac gaagcgctcc
     1021 tctcgactgt tgagacggcg ccgtaccgga aatcccggtt cccttcccag aaggatgttc
     1081 cgtccgatcg agagttgggg gacaaccacg atgtcctgag gcaccatgtt gaccagcctt
     1141 ccatcacccg gccgctccct ggcagagatg gatccgctgt ccggactgat cgtcccagca
     1201 agcaccttga ccagggtcga tttgccggcg ccattcagcc cgacgacacc atgcacccgc
     1261 ccggggatga tcgcaaggtc aaggtcctgt agcgcaatca cccccggata cgcctttgtg
     1321 acgccgagga gttcgatcaa tggttgaatc gctttcgcca ttgccacccg tgttcggggc
     1381 tcagccttcg tcgatcaact cattgtcggc gaccggtgtc ccgatctcgt cgaccgtgaa
     1441 caagggtgcg ggaagacaat tgacctgggg ctgctcctcg ccgttgagaa gtgagatcgc
     1501 tgttccgatt ccgacacgac ccatggaaac gggctctatt gcaaagaccg agtcgagcgt
     1561 tccggcctcg agcgccgcga tgctgtctgg ttcgtacccg aagctcacgt agggaatctc
     1621 tccctggtac tcggcgggga tcgccgatgg ctgcaggatg aagtagtgga cagacgcgat
     1681 ccagtcgatc tcagggaagg cctggactgc gtcctcgaac gacgccagcc cctccgcggg
     1741 atcgacgagt tgcgtcggcg cagcgacgat ctcgatgttc gggtactcag cggcgacctg
     1801 gtctgaaaac ccgacggtcc gcttccggga ccacgtggca ttggcgggac cagcgatgag
     1861 cacaccctgg ccaccttcct caccgactac ttcggcaaga cggtcggcca tcatctgacc
     1921 caggacgtag tcgtcttgca tcaccatgta gacgtcgccc tgcgccacct cggtggaaat
     1981 gttgaccacc gggatatcgg cgtcgacagc gaggtccacg gccggaaccg aaccttgaat
     2041 gtccgacgga gccatcacga tggcgtcggt ccctcccgcg atggcgtcct cgacctggct
     2101 gagctgcaga tcgggtgagg cataaccctc ggcggcgagg gtggtgacct cgacacccgc
     2161 ctcctcgccg gcg
//
LOCUS       DASTXO010000047         6534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1660431, whole genome shotgun sequence.
ACCESSION   DASTXO010000047 DASTXO010000000
VERSION     DASTXO010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6534
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660431"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..957
                     /locus_tag="VFU96_00925"
     CDS             37..957
                     /locus_tag="VFU96_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEU5111844.1"
                     /translation="MGGAGFLGSHVCTWLVYSGAQVTCIDNLITGQAHNVAHLMGDRK
                     FRLIDYDVTDYLHVPGDVDYVLNFASPASPVDYLKWPIHTLKVGAFGTHKGLGLAYHK
                     GARFFLASTSEVYGDPLVNPQPETYWGNVNPIGPRGVYDEAKRFAEAMTLAYNREHGV
                     DTRIVRIFNTYGPRMRADDGRAVPAFFKAALQNRPLPVFGDGSQTRSLCYVDDEVEGI
                     LRLLLSNETGPVNIGNPEEVTMLELAETVQDVVGTHPGIEFHPRPTDDPTVRRPDTSK
                     AERILGWKAQVTLREGLGRTLPWFKEILGG"
     gene            989..1663
                     /locus_tag="VFU96_00930"
     CDS             989..1663
                     /locus_tag="VFU96_00930"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5111845.1"
                     /translation="MTWSELSEWWLAEIEEDPAYEAAVTPMLLDVVRPEPGWTYLDLG
                     CGEGRVMRAITESGAAAHGVELTFALAQRAAKAGPVVIGRLPDLGFVRDDSYDGAFCV
                     LALEHIEDEGRFFAEVARAVRPGGVLALVVNHPYRTAPGATPISDVDGETLWRPGDYF
                     SSGTTLEPAGERHVLFHHRNTAIILESAAAAGWSLERFVEAPHHELVDQVGIPRLLAC
                     RWRLLP"
     gene            1670..3496
                     /gene="uvrC"
                     /locus_tag="VFU96_00935"
     CDS             1670..3496
                     /gene="uvrC"
                     /locus_tag="VFU96_00935"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU5111846.1"
                     /translation="MFERPKPSEIPDQPGAYFFRDKHGKVIYVGKAKSLRGRVASYFG
                     VGLHPRTQAMVDNAQSVEWIITESEVAALMLEFSLIKRHRPRFNIKLVDDKSYPYLAI
                     TRSDEWPRARVMRGAKRKGNEYFGPYAHTYAIRKTLDQLLRTFPVRTCTDSLYGRQRA
                     QGRPCLLYHIEKCSGPCIDAVTPEDYQQLVDGLAAFLRGDTEQVLTDLQVRMWEASER
                     QEFELAARHRDRIDDVRRALLRQEVVSERLEDFDLIAFHGDDLESAFQILHVRRGRLV
                     GRLGTVVDRVEELTDAELMGRVIWQLYGEDDPPRQVLVEIEPAEKELLQEWLAERRGT
                     KVSLRVPTRGSKRRLMETTVTNAAEAFARHRLKRSQDHNARAKALRSLQEVLDLPEPP
                     LRIEAFDISTLQGTNTVSSMVVLEDGLPKRSDYRRFKIRTIQGQDDFAAMEETIRRRF
                     RAFLAERNRKVAEQGRFSYPPSLVLVDGGAGQLGRAVKVLDELELDIPAIGLAKRMEE
                     VYLPGQPEPVQIPRDAEALYLLQQVRDEAHRFAITYHRQLRSKSMVDSILDEVPGIGP
                     KRKKDLLKRFGSLKKLREVEADSLAEVVPKNVAEDLYAALHS"
     gene            3509..4381
                     /gene="rapZ"
                     /locus_tag="VFU96_00940"
     CDS             3509..4381
                     /gene="rapZ"
                     /locus_tag="VFU96_00940"
                     /inference="COORDINATES: protein motif:HMM:NF003828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEU5111847.1"
                     /translation="MERPATDHPHVLVVTGMSGAGRSSAAKILEDLGYTVIDNLPPRL
                     LVPAAQSHDIPERHKHLAVVIDSRGGLPIEELQGAVKELSREGVLTRVVFLDAEDDVI
                     VSRYEENKRPHPLGHSTISESISAERDLLADLREEADVIVDTSSLNIHELRDRLTAQF
                     SEEEMARPMRVSIRSFGFKNGTPRDIDLLFDVRFLPNPHWIPELRPLRGTDERVAEYV
                     MSEEAAKEFVDRADEMLAFLIPHFEAEGKSYLSVGIGCTGGHHRSVAIAEELGRRLAQ
                     HGITATVRHRDIER"
     gene            4378..5355
                     /gene="yvcK"
                     /locus_tag="VFU96_00945"
     CDS             4378..5355
                     /gene="yvcK"
                     /locus_tag="VFU96_00945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HEU5111848.1"
                     /translation="MNPQTETETALAPLLLDPEGPVVAALGGGHGQAAALEAIQCYAG
                     EISALVAVGDDGGSSGRLSGLGIPPPGDVRRCLLALTPEPSLWSELFSHRFKGGDVAE
                     HSLGNLILAGLADLFGDFESAVSTAERMLGTVGSVIPVADQPITLTATIDGREVVGQK
                     TISLTSGELSELHIEPRDVVATQRALAAVAAADQIVIGPGSLYTSILSALKVNMLAPA
                     IMGAAAQRVFVLNLVTQDAETFGMSGAEHLEALAKHVGVTGPGIVVAHEGSLAVPDGH
                     QRVTIETEDAARHGWRVVFADVADETADWPEHDPLKLGRVLETLAVTED"
     gene            5361..6509
                     /locus_tag="VFU96_00950"
     CDS             5361..6509
                     /locus_tag="VFU96_00950"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU5111849.1"
                     /translation="MPQYLTLDDVEVNGLDVLVRSDLNVPIEGGVITDDFRIRAALPT
                     LERLVDSGARVSVCSHLGRPEGPDPAFSLRPVADAMAALTSKEVGFLEGGEITILENT
                     RFEAGETSNDPAQAARLADGFDLFVQDAFGSVHRAHASTVGIADLVKSVAGPLLDAEL
                     TALRRFLVDPQIPYVVVLGGAKVSDKLGVIENLLPRVDAMLIGGGMCFTLLKAKGVEV
                     GTSMVEEDMVDKVRGLVEGSHSEKIILPSDVVVADEFADGAEHKEVYVKDIPVGWMGL
                     DIGRETKQLFAAVIRTAETVFWNGPMGVFEWEPFRAGTEAVARGIAEATAFTAVGGGD
                     SVAALRMLGLESEVSHVSTGGGAGLELLEGKELPGVKVLEKWASGRDA"
BASE COUNT         1191 a         2033 c         2136 g         1174 t
ORIGIN      
        1 ggcttcaggg aactggaagg gaagcacgct gtggtcatgg ggggcgctgg gttcctcggg
       61 tcacacgtgt gcacttggct cgtctattcc ggcgcccagg tcacctgcat agacaacttg
      121 atcaccggtc aggcccacaa cgtggcccat ttgatggggg accggaagtt ccgcctgatc
      181 gactacgacg taaccgacta tctccatgtg cccggagacg tcgactatgt cttgaatttt
      241 gcctcgccgg cgtcacccgt cgactacctc aagtggccga tccacaccct caaggttggg
      301 gctttcggca cccacaaggg cctcggcctc gcttaccaca agggagcgag attcttcctg
      361 gcgtcgacct ctgaggtgta cggcgatcca ctcgtcaacc cgcagccaga gacctactgg
      421 ggaaacgtca acccaatcgg gccgagaggt gtgtacgacg aggcgaagcg cttcgccgag
      481 gcgatgaccc tcgcctacaa ccgggagcac ggcgtcgaca ccaggatcgt tcggatcttc
      541 aacacctatg gcccgcggat gcgcgccgac gacggacggg ccgtgcccgc gttcttcaag
      601 gctgccctgc aaaaccgccc tctgccggtg ttcggcgatg ggtcccagac ccggtctctc
      661 tgctatgtgg acgacgaggt ggagggcatt ctccgactcc ttctctccaa tgagaccggc
      721 ccggtgaata tcgggaaccc cgaagaggtg acgatgctgg aactggccga gaccgtccag
      781 gatgtggtcg ggacccatcc gggcatcgag ttccaccccc ggccgaccga cgacccgacc
      841 gtgcgtcgac ctgacacctc caaggcggag cggatcctgg gctggaaggc gcaggtcacc
      901 ttgcgcgaag ggctggggcg gactctgccc tggttcaagg aaatcctcgg cggctgacgg
      961 tcctgcccga ttgggccaca attcgacgat gacatggtcc gaactgtcgg aatggtggct
     1021 tgccgagatc gaagaagacc ccgcctacga ggccgcggtc actccaatgc tcctcgatgt
     1081 ggtccgaccc gagccgggct ggacctatct cgacctcgga tgtggcgaag ggcgggtgat
     1141 gagggcgatc acagaatccg gggcagccgc tcacggtgtc gagctcacct tcgccctcgc
     1201 ccaacgagcc gccaaggcag gaccggtcgt catcggacgg ttgcctgatc tcggctttgt
     1261 gcgcgacgat tcctatgacg gggcattttg tgtcttggcc ctcgagcaca tcgaggacga
     1321 gggacgtttc tttgcggaag tcgcacgcgc cgtccggccc ggcggagtcc tggcactagt
     1381 ggtgaatcac ccgtatcgga ccgctcccgg ggccactccg atctccgatg tcgatgggga
     1441 gactctgtgg cggccgggcg actacttctc ttcggggacg actctggaac cagctggcga
     1501 gagacacgtg ctgtttcacc atcggaatac ggccatcatc ctcgagtcgg ccgccgctgc
     1561 agggtggtct ctggagcgat tcgtcgaggc tccccaccac gaactcgtcg accaggtcgg
     1621 gatcccgagg ctgctggctt gccggtggcg cctcctaccc tgagaaggcg tgttcgaacg
     1681 accaaaacca tcggagatac ctgaccagcc gggcgcctac ttcttccgtg acaagcacgg
     1741 caaggtgatc tatgtcggca aggccaagag cctccgcggc cgggtcgcct cgtacttcgg
     1801 agtcggcctt cacccgcgca cacaggcgat ggtcgacaac gcccagtccg tggagtggat
     1861 catcaccgag tccgaggtcg cggcgctgat gctcgagttc tcgctgatca agaggcaccg
     1921 gccccgcttc aacatcaagc tcgtcgacga caagtcgtac ccatatctcg ccattaccag
     1981 gagcgacgaa tggccacggg cccgggtgat gcggggtgcc aagcggaaag gcaatgagta
     2041 cttcgggcct tatgcccaca cctacgccat ccgcaagacc ctcgaccaac tgcttcgcac
     2101 cttcccggtc cgcacctgca ccgactcgct gtacggccgt cagcgcgccc agggccgtcc
     2161 gtgtctgctc taccacatcg aaaaatgctc cggcccctgc atcgacgccg tcactccaga
     2221 ggactatcag caactggtcg acgggctcgc tgcgtttctg cggggagaca ccgagcaggt
     2281 gctgaccgac ctccaggtcc gcatgtggga ggcgtcggag cgtcaggaat tcgagctcgc
     2341 ggcccggcat cgggatcgga tagatgacgt tcgtcgggct ctccttagac aggaggttgt
     2401 ctcggagcgg ctggaggact tcgacttgat cgccttccat ggggacgatc tcgagtcggc
     2461 gttccagatc ctccatgtca gacggggccg tctcgtcggc aggctgggta ccgtcgtgga
     2521 tcgagtcgaa gagctgaccg acgccgagtt gatggggcga gtgatatggc agctctacgg
     2581 ggaggacgac ccgcctcgac aggtcctcgt cgagatagag cccgccgaga aggaattgct
     2641 ccaggaatgg ctggcggagc ggcgagggac aaaggtgagc ttgcgcgtgc cgacgagggg
     2701 gtcgaagcga cgtctcatgg agacaaccgt gacgaacgcg gccgaggcgt tcgcgcggca
     2761 ccggctcaag cgcagccagg atcacaacgc gagggccaag gcccttcgct cgctgcaaga
     2821 ggtcctcgat cttcccgaac cgccccttcg catcgaggct ttcgacatct cgactttgca
     2881 ggggacaaac accgtctcgt cgatggtcgt gctcgaggac ggattgccga agaggtctga
     2941 ttaccgaaga ttcaagatcc gaactatcca ggggcaggac gacttcgccg ccatggagga
     3001 gacgattcgc cggcgttttc gggcattctt ggcggagagg aaccgcaagg tcgccgagca
     3061 ggggcgattc tcatatccgc cgtctctggt gttggtcgac gggggagcgg ggcagctggg
     3121 acgggcggtg aaggtcctcg atgagctcga gttagacatt cccgcgattg gtctcgccaa
     3181 gcggatggaa gaggtatatc tgcccgggca accggagccg gtacagattc cccgcgatgc
     3241 cgaggccctc tatctgctgc agcaggtccg ggacgaggct caccgattcg cgatcaccta
     3301 ccaccgccaa cttcgctcca agtcgatggt cgactcgatc ctcgacgagg ttcccgggat
     3361 cggccccaaa cggaagaagg acctcttgaa gcgtttcggg tcgttgaaga aactgagaga
     3421 ggtagaggcg gattcgctgg ccgaggtggt gccgaagaac gtggccgagg atctgtacgc
     3481 tgcattgcac agctgatcga gagcaccgat ggagcgcccc gcaaccgatc atccacacgt
     3541 tctcgtggtc accggcatgt ccggcgccgg acgttcatcc gcagccaaga tcctcgaaga
     3601 cctcggctac acggtcatcg acaacctccc cccgaggttg ctggtcccgg cggcgcaatc
     3661 ccatgacatc ccggagcgtc acaagcacct cgccgtagtg atcgactcca gggggggtct
     3721 ccccatcgag gagttgcagg gtgcggtcaa ggagctgagc agggaggggg tgctcacgag
     3781 ggtcgtgttc ctcgacgcgg aggacgacgt catcgtctct cgctacgagg agaacaagcg
     3841 gccccacccc ctcggccatt cgacgatctc cgagtcgatc tcggcggaac gggacctcct
     3901 cgccgacctc cgcgaggagg cggacgtgat cgtcgacacg tcgtcgctca acattcacga
     3961 gttgcgggac cggctcactg cccagttcag cgaggaggag atggctcgcc cgatgcgggt
     4021 ctcgatccga tcgtttggct tcaagaacgg gacaccacgc gacatcgacc tcctcttcga
     4081 cgtgagattc ctccccaacc cccattggat tcctgagctg cgccctctgc ggggcaccga
     4141 cgagcgggtt gccgagtacg tcatgagcga ggaggcggcc aaggagttcg tggatcgggc
     4201 cgacgagatg ctggcgtttc tcatcccgca tttcgaagcc gaggggaagt catacctatc
     4261 ggtcggcatc ggctgcaccg ggggtcatca tcgctccgtg gcgattgccg aagaactggg
     4321 ccggcggctg gcccagcacg ggatcaccgc cacggtccgt caccgcgaca tcgaacgttg
     4381 aatccacaga ccgagaccga gacggcgctc gcccctcttc tcctcgatcc cgagggaccg
     4441 gttgtggccg ccctcggcgg cgggcacggg caggccgcag cactcgaggc catccagtgt
     4501 tacgccgggg agatatcggc cctggtcgcg gtcggagacg acgggggctc gtcggggagg
     4561 ctcagtggcc tcggaatacc gccgcccggt gacgtccgcc gttgcctgct tgccctcacc
     4621 cccgagccat cgctctggtc cgagctgttc tcccaccggt tcaagggtgg tgacgtggcg
     4681 gagcactccc tcggcaactt gatcctggcc ggactcgccg atctgttcgg tgatttcgag
     4741 tcggcggtat cgacggcaga gcggatgctc gggaccgtgg gctcggtgat cccggtggca
     4801 gaccagccga taacgctcac ggcgaccatc gacgggcgcg aggtggtcgg ccagaagacc
     4861 atatcgctga catcaggcga gctatccgag ctccacatcg agccgcgaga tgtcgtggcc
     4921 acccaacgtg ccctcgccgc tgtagccgca gccgaccaga tagtgatcgg gccgggcagc
     4981 ctctacacct cgatcctgtc cgccctcaag gtgaacatgc tcgccccggc gatcatgggg
     5041 gcggctgccc aaagagtgtt cgtcctcaac ctggtcactc aggatgctga gacgttcggt
     5101 atgagcggtg cggagcatct cgaggcgctc gccaaacacg tcggggtcac cggccccgga
     5161 atcgtcgtgg cccacgaggg aagcctcgca gtccccgacg gtcaccagcg tgtgacgatc
     5221 gagaccgagg acgcggcccg tcacgggtgg cgtgttgtct ttgcagatgt ggccgatgag
     5281 actgcagact ggcctgagca cgacccgctc aagctgggca gagtgctgga gacactggcc
     5341 gtcaccgagg actgagacgc atgccgcaat acctgaccct cgacgacgta gaggtcaacg
     5401 ggctcgatgt tcttgtgcga tccgacctca acgtcccgat cgagggcgga gtcatcaccg
     5461 acgacttcag gatcagggcg gctttgccca cgttggagcg tctcgtcgat tcgggtgccc
     5521 gggtgagcgt ctgctcgcat ctcggtcgtc cggaaggtcc cgatcctgca ttctcacttc
     5581 gcccggtggc cgatgccatg gccgccctca cttcgaagga ggtgggattt ctcgagggcg
     5641 gggaaatcac cattcttgag aacacgaggt tcgaggctgg ggagaccagt aacgatcctg
     5701 cgcaggctgc ccggctcgcc gatggtttcg acctcttcgt gcaggacgca ttcggatctg
     5761 tgcatagggc gcatgcctcg acggtcggaa tcgccgattt ggtcaagtcg gtcgcaggcc
     5821 cgctactcga tgccgagctc acggctctga ggcggttcct ggtcgaccca cagattccct
     5881 acgtagtcgt tctgggtggc gccaaggtct cagacaagct gggtgtcatc gaaaacctcc
     5941 ttcccagggt cgatgcgatg ctcatcggcg gggggatgtg cttcaccctg ctcaaggcga
     6001 aaggtgtgga ggtcggcact tcgatggtcg aggaagacat ggtggacaag gtgcggggcc
     6061 tcgtcgaggg gtcgcattcc gagaagatca tccttccctc cgacgtcgtg gtcgccgacg
     6121 aattcgccga tggcgccgag cacaaagagg tgtacgtgaa ggacatccca gtgggatgga
     6181 tgggtctcga catcggccgg gagacgaagc agttgttcgc agccgtgatc cgcaccgccg
     6241 agaccgtttt ctggaacggg ccgatgggtg tgttcgagtg ggagccgttt cgtgccggaa
     6301 ccgaggcggt ggcccgtggc atcgccgagg ccactgcctt caccgcggtg ggtggcgggg
     6361 attcggttgc ggcgctccga atgctcgggc tcgaatctga ggtatcccat gtgtcgaccg
     6421 gcggaggcgc cgggctcgag ttgctggagg gcaaggagct tcctggcgta aaagtcctcg
     6481 aaaagtgggc cagtggtcgg gatgcctgag cgcaagagcc tgatagtcgg caac
//
LOCUS       DASTXO010000048         4135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417 k141_2940,
            whole genome shotgun sequence.
ACCESSION   DASTXO010000048 DASTXO010000000
VERSION     DASTXO010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2940"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VFU96_00955"
     CDS             <1..831
                     /locus_tag="VFU96_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEU5111850.1"
                     /translation="DDIWVDRGGSLTRVEARFSGEADLYATVERVIAPLGLRVDRSSP
                     MVDGRLIDGSRIHVVVPPAAVDHPIVAIRRFTKALGSLEELMTVGTASREQVEVLSTA
                     VKAKRTVIVSGGTGTGKTTLLDLLAGLISPEERVVTIEDAAELQIPGHRVRLEAHPSN
                     PEGVGEITIRQLLRSALRLRPDRIIVGEVRGPEALDLIWALNTGHRGSMSTIHANTPQ
                     EALWRLETLALSAGDTSELAVRRQLHAAIDIVVQMERHDGNRVISDMAFVGNEGLEAG
                     "
     gene            831..1472
                     /locus_tag="VFU96_00960"
     CDS             831..1472
                     /locus_tag="VFU96_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111851.1"
                     /translation="MTWVLVAFAVASGVDWRRVALLAMAVAAPLLMAGLITVFWWRAR
                     PALSMRATRFCEAVSGELRSGATLRASLEAAAVSVEATEISRCCRQGAPMDQIAMVAR
                     HEFSEIGPELGTLLGRADGLGVSPAALFDEIGALALAQVEVAHEVSIASAPAKATGAV
                     LLLVPILAMGWAFTRGSLKELLQQPAQRGAVLSGLALVWAGLMLSGLILRRAR"
     gene            1469..2008
                     /locus_tag="VFU96_00965"
     CDS             1469..2008
                     /locus_tag="VFU96_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111852.1"
                     /translation="MSAVVSSSIGLVVAGLPGAALAICVNAAFRLRRRLIKPPALIRP
                     VLIVVLIELRSGRSVLGALQTVATAFPDRPELTLAARVATISGLSHASLMAIGEIRGV
                     LSHLARAQKSGAALGDTVRSMIEADIAAEKARRITRARSLPVRLMLPITLLLLPGLVL
                     LLYAPSMLRLFEDLSGPLT"
     gene            2060..2251
                     /locus_tag="VFU96_00970"
     CDS             2060..2251
                     /locus_tag="VFU96_00970"
                     /inference="COORDINATES: protein motif:HMM:NF025395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4244 domain-containing protein"
                     /protein_id="HEU5111853.1"
                     /translation="MARMLTWLATRDDRGQGTVEYFLVILAATALAVIALRWLQSGSG
                     GGLLGSLFGKVIDWVAGRF"
     gene            2269..2613
                     /locus_tag="VFU96_00975"
     CDS             2269..2613
                     /locus_tag="VFU96_00975"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HEU5111854.1"
                     /translation="MTVELFLVLPIAILVMVASIQLIGVARARIELQGAVREGARVAA
                     TTPDPAKAIDAVLAALTPAMRDRARVSVERPSRVGAVATVTVSMRHVLGPPFPRSLGV
                     ELSARATMRTER"
     gene            2610..2996
                     /locus_tag="VFU96_00980"
     CDS             2610..2996
                     /locus_tag="VFU96_00980"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3654c family TadE-like protein"
                     /protein_id="HEU5111855.1"
                     /translation="MIGDRGAGTILMIGFCALVAAVGVATTALGVLFDAREQAATAAE
                     AAALAAAVATYPPAGADDPGDVAGEYAVRNGARVVSCSCQTDPTLQPRVVAVTVALRA
                     DVPMFGEVLVGKTARAEFEPRAWLGR"
     gene            3025..3396
                     /locus_tag="VFU96_00985"
     CDS             3025..3396
                     /locus_tag="VFU96_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019762637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5111856.1"
                     /translation="MSDVQPVDRFIETLEARDWEAWASLLDPDVVYEIPQSRERIRGR
                     DRYLQFNQEYPGDWHLRLKVSIADARNGVAWFGWEVEGSEPADGIAFFTLSDGLITSV
                     TDFWPEAYEPPPGREHLVERW"
     gene            3398..3880
                     /gene="ybaK"
                     /locus_tag="VFU96_00990"
     CDS             3398..3880
                     /gene="ybaK"
                     /locus_tag="VFU96_00990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEU5111857.1"
                     /translation="MTGVVTRATVVLDDAAAIYRVHRYDVTEKVGDGYGEAVAAAIGM
                     GPARVFKTLVAEVDEAPVIAVIPVDRRLATKKLARAIGGKHCSVVAPAIAERETGYVT
                     GGISPFGQRKRHPLLLDSSAEHHETIAVSGGRRGLQLELSPQTLLDLTGGVLAVIVDP
                     "
     gene            3905..>4135
                     /locus_tag="VFU96_00995"
     CDS             3905..>4135
                     /locus_tag="VFU96_00995"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEU5111858.1"
                     /translation="MATRLEVLRMLAELAKLTALDEGSPQAFRVRAYENALHGIEAYQ
                     GELTGLGKKELTEIKGVGASTADKILEYMSTGS"
BASE COUNT          643 a         1299 c         1433 g          760 t
ORIGIN      
        1 gacgacatct gggtggaccg gggcgggtca ttgacgcggg tggaggcgcg attcagcggg
       61 gaggccgatc tgtacgccac cgtcgagaga gtcattgccc ctctcgggct gagggttgat
      121 cggtcgtcgc cgatggtgga cggtcgtttg atcgatggga gccggatcca cgtagtggtt
      181 cccccagccg ctgtcgacca ccccatcgtc gccatccgac gcttcaccaa agcgctcggg
      241 tcgctcgaag agctgatgac ggtcgggacg gcaagtcgag agcaggtcga agtgctctca
      301 actgcggtga aggcgaaacg aacggtcatc gtctcgggtg gcaccggcac cggcaagacc
      361 acgctgctcg atctcctggc aggcctcatc tcgccggagg agagggtggt gaccatcgaa
      421 gatgccgccg agctccagat accgggccac cgcgtgcgtc tcgaggctca cccgtcgaac
      481 ccggagggcg taggcgagat aacgatccgt cagctgctga gatcggcgct ccgtcttcgc
      541 cccgatcgga tcatcgttgg cgaggtgcga ggcccggagg ccctcgacct gatctgggca
      601 ctcaacacgg gtcaccgggg ctcgatgtcg acaatccacg ccaatacccc tcaggaggcg
      661 ttgtggaggc tggagacact cgccctctcg gccggtgaca cgtcggagct ggccgttcgc
      721 cgtcagcttc atgccgcgat cgacatcgtg gtgcagatgg agcggcacga cggcaaccgg
      781 gtgatctccg atatggcatt cgtcgggaac gagggcctcg aggccggcta gtgacctggg
      841 ttcttgtcgc gttcgcggtg gcttcggggg tcgactggcg ccgtgtcgca cttctcgcca
      901 tggctgtagc cgccccccta ctgatggccg gtctgatcac cgtcttttgg tggcgggcac
      961 gaccagctct ttccatgcgc gcgaccaggt tctgtgaggc ggtgtccggc gagcttcgat
     1021 ccggggccac gctccgcgcc tctttggaag cggccgcggt ttcagtggag gcgaccgaga
     1081 tttcccgatg ctgccgtcaa ggggccccga tggaccagat cgccatggtg gcgaggcacg
     1141 agttcagcga gatcggcccc gagctcggaa cgctcctcgg gcgtgccgac gggctcggcg
     1201 tgtcgcctgc ggcgttgttc gatgagatcg gtgctctggc cctggcccag gtcgaggttg
     1261 cccatgaggt gtcgatcgca tctgcgccgg cgaaggcgac gggtgcggtg ctcctcctgg
     1321 tcccgatcct cgccatgggc tgggcgttca cacggggcag tctcaaagag ctcctccagc
     1381 agccggccca acgaggcgcg gtcctgagtg ggctcgcttt ggtttgggcc ggtctcatgt
     1441 tgtcggggct gattctccgg agagccaggt gagcgcggtg gtgtcgagct cgatcgggct
     1501 ggtggtggca ggactgcccg gcgcggccct cgccatctgt gtgaacgccg cttttcgact
     1561 tcgccggcgg ctcatcaagc caccagccct tattcggcct gtactgatcg tcgtcctcat
     1621 cgagcttcgg tcaggccgtt cggtgctcgg ggcgctgcag acggtcgcca ctgcatttcc
     1681 cgaccggccc gaactgacac tcgcagcgcg agtggccacc atctctggcc tcagccacgc
     1741 ctcgctgatg gcgatcggcg agatcagggg agtcctgtct caccttgccc gcgctcagaa
     1801 gtcgggcgcc gccctcggtg acacggttcg gtcgatgatc gaggcggaca tcgccgccga
     1861 gaaggcgaga cgcatcaccc gggcgcgttc cctgcccgtg cgcttaatgc tcccgataac
     1921 cctgttgctc ctgcccggtc tggttctcct cctctacgca ccttcgatgc tccggctttt
     1981 cgaggacttg tccgggccgt tgacatgaga tccgcacttc tccccgcgcc ggtcggaaac
     2041 cggagggaaa ggagaggaaa tggcacggat gctgacctgg cttgcgaccc gagacgaccg
     2101 cgggcagggg acggtcgagt acttcctggt gatcctcgcc gccaccgcgt tggccgtgat
     2161 cgccctgcga tggctgcagt cggggagtgg cgggggtcta ctcgggagcc tgttcggaaa
     2221 ggtgatcgac tgggtggcgg gacgattctg agcgaggagc gcggctcggt gacggtggag
     2281 ctgttcctcg tgctccccat tgcgattctc gtgatggtgg cgtcgattca gctgatcggc
     2341 gtggctcggg cacgaatcga gctgcagggc gcggttcggg aaggggcacg cgtcgccgcg
     2401 acgacgcctg acccggccaa ggcgatcgat gcggtgctcg cagccttgac gcctgcgatg
     2461 cgggaccgtg cccgcgtttc ggtcgaacga ccgtcgaggg tcggcgcggt cgcgaccgtc
     2521 acggtctcga tgcggcatgt gctcggcccg ccgttcccgc gcagtctcgg ggtcgagctg
     2581 tcggctcggg cgacgatgcg aaccgagaga tgattggcga ccggggagcg gggacgatcc
     2641 tgatgatcgg tttctgcgcc ctggtcgccg ccgtcggcgt tgccaccacc gcgttgggcg
     2701 tcctgttcga cgcccgcgag caggcggcaa cggctgccga ggccgctgct ctcgccgccg
     2761 cggtcgccac ctatccgccg gccggcgccg acgacccggg cgatgtcgcc ggggaatacg
     2821 cggtccgcaa cggggcgcgg gtggttagct gctcttgcca aactgacccc acgctccagc
     2881 cgcgggtggt ggcggtcacc gttgcattgc gtgccgatgt gccgatgttc ggtgaggtcc
     2941 tggtgggaaa gacggcccga gcagagttcg agccacgagc ttggctgggc cggtagccgg
     3001 acactccggt aggttcgcgc tgctgtgtca gacgtgcagc cagtcgaccg cttcatagag
     3061 accctggaag ctcgtgactg ggaggcatgg gcctccctgc tcgatcccga tgtcgtctac
     3121 gagatcccac agtcgagaga gcgaatcaga ggacgtgacc gatacctcca gttcaaccag
     3181 gagtatccgg gcgattggca tcttcgtctc aaggtctcaa tcgccgatgc ccgtaacggc
     3241 gtcgcttggt tcggttggga ggtcgaaggc tccgaacccg ctgatgggat tgcgttcttc
     3301 accttgtcgg atgggctcat cacctcggtg accgacttct ggcccgaggc ctatgaacca
     3361 ccaccggggc gcgagcatct cgtcgagcgg tggtgagatg accggggtag ttaccagggc
     3421 cactgttgtc ctcgacgacg ccgccgcaat ctaccgggtc catcgttacg acgtaacaga
     3481 gaaggtggga gatggctatg gggaggccgt cgcggccgcc atcgggatgg ggcctgcccg
     3541 agtcttcaag acgctcgtgg ccgaagtgga cgaagcgccg gtgatcgcgg tgatcccggt
     3601 ggaccgacgt ctcgccacca agaagttggc tcgagccatc ggtggcaagc attgctcggt
     3661 ggtggcgccg gcaatcgccg agcgtgagac cggctatgtc accggtggca tcagcccgtt
     3721 cgggcagcgc aagagacatc cattgctcct ggactcctcg gcggagcacc acgagacgat
     3781 tgctgtatcc ggaggacggc gcgggctcca actcgagctg tctccccaaa ctcttctcga
     3841 tctgaccggt ggggtcttgg ccgtcatcgt cgacccgtga ggacgcggac ctaacctccc
     3901 gcccgtggcc acccgtctcg aagtcctgcg catgctggcc gagctcgcca agctcaccgc
     3961 gctggatgag ggcagtcccc aggcattcag ggtcagggcc tacgagaacg ccctgcacgg
     4021 gatcgaggca tatcagggcg agctgacagg tctgggcaaa aaggagctga ccgagatcaa
     4081 gggagtcggc gcctcgacgg cagacaagat cctcgagtac atgtccaccg gctcg
//
LOCUS       DASTXO010000049         3648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1255366, whole genome shotgun sequence.
ACCESSION   DASTXO010000049 DASTXO010000000
VERSION     DASTXO010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255366"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..804)
                     /locus_tag="VFU96_01000"
     CDS             complement(67..804)
                     /locus_tag="VFU96_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111859.1"
                     /translation="MTGMHRSGTSLVAMTLNALGLDFGDDDEFYAADEWNQKGYFERR
                     DVVDLNSRLITGFTRTQGVTSSSLGKIRYLTQPSPEVIATRGNALKGEIEHTALEIGD
                     LHVKDPRFVLTVDSWRPHVSQIVVCLRRPDQVAFSLKRRQRVPLGISYGFWNYHARAI
                     LAIAHDRMHYVDFDVLAGGAPEAELVGLARFLGIESTSPELVATLRAHFTPTLKHFDP
                     SDAVDLPSPTRDLWEQLSARRTSLANG"
     gene            945..1790
                     /locus_tag="VFU96_01005"
     CDS             945..1790
                     /locus_tag="VFU96_01005"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5111860.1"
                     /translation="MDTKGPDQPRGAHGDRLVIFRDLLSALPPGRLLDLATGHGGFAL
                     IAQELGWEVTAVDARTERMPMTPGIEWVQADVRQFPTDGYDVITILGLLYHLELEGQA
                     DLLRRCSSTLTLIDTHVSMRPTTLMEGYFGHFFEEILSAHTASWGNPLSFWVTEESLY
                     RLLLTSGYASVLKVLPPPVTADRTFHVAAPESDPNLEDLKGRFRSSNGTYRLEPRLEN
                     VSVGSIDDHMYQLVSDMATLRQERDSARDALSRLRNRKSVRLALQGASLVKPLYRQNK
                     ESPSD"
     gene            1843..3519
                     /locus_tag="VFU96_01010"
     CDS             1843..3519
                     /locus_tag="VFU96_01010"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEU5111861.1"
                     /translation="MIVYTAIFNNYDLLQSPPPVEGVRYVCITEERPPSPNGWELHLV
                     EGSVLPHPYAQRAYKILAHRVFPEADVSLYLDGTFELLVDPRRLVERYLADTDLALFR
                     HPQRDGVYSELSACADLGKERKEITTLTEQRYRAQGMPETGYLHAAGVLLRRHTDEIG
                     DFNERWMMEVIDSSVRDQPALANTLWRTGVPVATIDEDIWNSKLVAYHPHRDKMLPEG
                     RQYLFMGGNPRSGTTALCDLLNRDRRIILGMERYRRIREEITPDHFTRSKFFSPDESE
                     TTFLPKRLMPLDRPGFKVWPEDEDLVREKWNSPDLRYVGDKSPFYVLQLPYLAKTFPG
                     SRFLIAIRDPVSVADSYQRRAEDPNDHWPVENDYRLAIEHWNGSVAALLAYLENHGFG
                     DLFLIDYDTFYSGDPDYLRSLYSFLELEMESEVLQAFETMTADWADHVTRPSRLSSVM
                     EEEVRSNADWERYSQVRRALPMMRDYRLLRAERRLLLDEEQARRDEARAFHRMFDFTR
                     ELYGKIQAVETDGSERPAVTEAVSEVASMWRSFWAVPELEDPQDVEPIAD"
BASE COUNT          721 a         1127 c         1092 g          708 t
ORIGIN      
        1 ttccacccgg agccgctccg aaccgggata tcgcgattgc tcaacaagta tctgagccga
       61 cgctgatcat ccgttggcga gcgaggtgcg cctcgccgat agctgctccc ataggtcgcg
      121 agtgggtgac ggaaggtcga ccgcatcgga tgggtcgaaa tgcttcagcg tcggggtgaa
      181 gtgagcccgc aacgtggcga caagctccgg agaagtggac tcgatgccga ggaaccgggc
      241 caggccgacg agctcggcct cgggggcccc accggcgagg acatcgaagt ccacgtagtg
      301 catccggtcg tgggcgatgg ccaggattgc ccgggcgtgg tagttccaga agccgtacga
      361 aatccccagc ggcacccgct ggcgtcgctt gagggaaaat gccacctgat ccggccggcg
      421 cagacagacc acgatctggc tcacgtgggg gcgccaggag tcgacggtca ggacgaaccg
      481 aggatccttc acatgcagat ccccgatctc caaagccgta tgctcgatct cccctttcaa
      541 cgcgttgccc ctcgtcgcga tcacttcggg tgagggctgt gtcaggtacc gaatcttgcc
      601 aagcgaggac gaggtcacac cctgggttcg agtgaaacca gtgatgagcc ggctgttgag
      661 gtccacgacg tcgcgtcgct cgaaatagcc cttctgattc cattcgtcag ccgcgtagaa
      721 ctcatcgtcg tcaccgaagt cgagacccag ggcgttcagg gtcatggcga caagggaggt
      781 tcccgatcga tgcattccgg tcacgaccct caccggctca ttatggctcg acgtccctct
      841 cggaacgccg ctgaccgtga cggccgttgc cactgtgacc accggtaccc tcgggaaagc
      901 ccatccatgc gtcacggggg ttccctccat cgaaaggccg gctcttggac acgaaaggac
      961 ctgatcagcc acgcggcgct catggcgacc gtctcgtgat cttccgcgat ctgttgagcg
     1021 cgctgcctcc gggtcgcctg ctcgatctgg ccaccgggca tggcgggttt gccctgatcg
     1081 cccaggaact cgggtgggaa gtcacagctg tcgacgccag gaccgagcgc atgccgatga
     1141 ctcctggtat cgagtgggtt caagccgatg tcaggcagtt cccgaccgac ggatacgacg
     1201 tgatcaccat cctggggctc ctctaccacc tcgagctcga gggacaagcc gatctcctgc
     1261 gtcgctgctc ttcgacgctc actctcatcg acacccatgt ctcgatgcgc cctaccacgc
     1321 tgatggaggg ctatttcggc catttcttcg aggagatatt gtcggcgcac accgcgtcct
     1381 ggggcaatcc actctcgttc tgggtgactg aggaatctct ctaccggctc cttctgacga
     1441 gtgggtacgc ctcagtgctc aaagtattgc ctccgccggt caccgctgat cgcacgtttc
     1501 atgttgccgc ccccgagtcc gatccgaacc tcgaggactt gaagggaaga tttcggagca
     1561 gcaacgggac ctaccgcctc gagcctcggc tggagaacgt atcggtcggt tccatcgacg
     1621 accatatgta ccaattggtg tccgacatgg cgacactccg ccaggagcgt gacagtgctc
     1681 gtgacgccct gtcacgtctc cgcaatcgca agtcggtgag attggcgctg caaggcgcat
     1741 cgctggtcaa gccgctctat aggcagaaca aggaatcgcc gagcgactga cctttggcca
     1801 gaacataggc tgtgtcgctg tcactcggga gctcggcgat cgatgatcgt ctacacggcg
     1861 atattcaaca actacgacct cttgcaatct ccacccccgg tagaaggggt tcgctacgtc
     1921 tgcatcacgg aggagcgacc ccccagccca aacggctggg agttgcacct ggtggaaggg
     1981 tccgttctcc cgcatccata cgcacagcgc gcttacaaga tcctcgccca tcgcgtcttt
     2041 cccgaagcgg acgtcagttt gtacctcgac ggcacattcg agttgttggt agatccacgt
     2101 cgcttggtgg agcgttacct ggctgacacc gatctcgctc tgttccgaca cccacaacga
     2161 gacggcgtgt actccgaatt gagcgcgtgc gccgatctcg gcaaggagcg caaggaaatc
     2221 acgactctga cggagcagcg gtaccgagcg cagggaatgc ccgagaccgg atatctgcac
     2281 gcggccggcg tcttgctcag aagacatacc gacgagattg gcgacttcaa cgagcgttgg
     2341 atgatggaag tgatcgactc cagtgtccgc gatcagcccg cgctcgccaa cacgctgtgg
     2401 agaactggag ttccggtggc caccatcgac gaggacatct ggaacagcaa gctcgtcgcc
     2461 taccacccac atcgggacaa gatgctcccc gagggtcggc agtatctatt catgggcggc
     2521 aatccacgat cgggaaccac agccctctgt gacctgctca accgcgaccg gcggatcatc
     2581 ctcggcatgg agcggtaccg tcgaatcagg gaggagatca cgcctgatca cttcacccgg
     2641 agcaagttct tctcacccga cgagtcggag acgacttttc tacccaagag gctcatgcca
     2701 ctcgatcggc ccgggttcaa ggtttggccg gaggacgaag atctggtcag ggagaagtgg
     2761 aactcaccag atctgcgata cgtgggggac aaatctccct tctacgtcct tcaactgccc
     2821 tacctggcca agaccttccc cgggtcgcgt ttcctgatag caattcgaga tccagtgtcg
     2881 gtcgcagatt cgtatcagcg tcgtgccgag gacccaaatg atcactggcc ggtggagaac
     2941 gactaccgac tggccatcga gcactggaat ggcagtgtcg ccgccctgct ggcatacctc
     3001 gaaaaccatg gttttggtga cctcttcctg atcgattacg acaccttcta ctcgggagat
     3061 ccggattatc tgcgttccct ctattcgttc ctcgagctcg agatggaaag cgaggtcctc
     3121 caagccttcg agaccatgac cgctgattgg gccgaccacg tgacccggcc ctcccgcctc
     3181 agctcggtca tggaggagga agtgaggtcg aacgctgatt gggagcgtta ctcacaggtg
     3241 aggcgagcgc ttccgatgat gcgtgactat cgcctgcttc gcgcggaacg ccggctgctt
     3301 ttggacgagg agcaggcccg tcgagacgag gcgagggctt ttcatcgcat gttcgatttc
     3361 actcgtgagc tttacgggaa gattcaggca gtagagacgg atggatcgga acgccccgcg
     3421 gtcactgaag ccgtctccga agttgcgtcg atgtggcggt ccttctgggc cgtgcccgag
     3481 ctcgaggacc cgcaggatgt cgagccgatc gccgattgac gtggccttgg ttagaggtgc
     3541 ccgctagccg gtcagggacc gacgaaggaa cgccgccatc tggccccggg tgaccaactc
     3601 agtcgggcaa aaccacgtgt tgtcaggcgg gttgcacccc aatgttat
//
LOCUS       DASTXO010000050         2975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1476723, whole genome shotgun sequence.
ACCESSION   DASTXO010000050 DASTXO010000000
VERSION     DASTXO010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476723"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VFU96_01015"
     CDS             <1..632
                     /locus_tag="VFU96_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013038183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU5111862.1"
                     /translation="GRRAGQIAATRAAAILRYAGFEDVRLLDGGYDHWVRSGLGLETE
                     RRVPTPIDEFGVAIPRRPELIIDIEDAKSILADPAGAALVSVRTWKEHIGKVSGYNYI
                     EPKGRISGDVWGNCGTDAYHMQHYRNIDNTMRAYPEIAANWNEAGITPDKWVAFYCGT
                     GWRASETWFYAYLMGWDQIAVYDGGWFEWSQDPVSNPIEVGEPEDEAAA"
     gene            745..2112
                     /locus_tag="VFU96_01020"
     CDS             745..2112
                     /locus_tag="VFU96_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111863.1"
                     /translation="MQTLIGIGEGGDVTQETDFDLRDYVYIIALAPAILGTHAFAVRL
                     SRTVELTGRLQGRSTVNLLSGGLALLNLFATAAICRLSGIPLGMEEGAGVIDVILAAL
                     IALAVGFVLCSVAAMTVRFLPDRRRTGPNRRANSFLALLTTATKVLLSLVVVLLAISL
                     IGAYIEQFELGFGALGPMDWAGLFLAPVLLVALALYIRRGQRKATSYAKRLRPSSEDS
                     VEGEPRVLYLRPFHEEHRLFAGSQTLDEFLGDEIAKQIGPLIALGNPTDRISPDGAER
                     QYYQDDDWQSAVEALALEAPCIVAATSASPNTAWELRRVLELGLERRIYLLSPPPREA
                     ETPPTASDEEAGPIRRALRIARRATFSWLAQDLDGLSREVSLGQLPSALSPAGMTPWH
                     ELVETLAACGYSVHFSDPGPGTVIGFETGGMAVILEQGATSPIDYIKPIVDRSVRPQP
                     PGPQP"
     gene            2212..>2975
                     /gene="chrA"
                     /locus_tag="VFU96_01025"
     CDS             2212..>2975
                     /gene="chrA"
                     /locus_tag="VFU96_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEU5111864.1"
                     /translation="MDNTPPTYGLWGLVRYFLYLGTAGFGGPVALVEYMHRDLVEGRR
                     WITEDEYREGLALANVSPGPLAAQLAIYLGYVHYSFLGATLVAVAFVLPSFLMVVAIG
                     WAYLEYAGLPWMQAVFYGVAAAIIGIIAWSAYRLTSKTISSDLLLWVILVSMAAITAI
                     TETEHIILFPIAGLAVWIARNPRIRRRDTVNGVLLLPLALQSAVADPSAGEIFWFFFK
                     AGAFVFGSGLAIVPFLYGGVVVEYGWLDSQEFLDAVA"
BASE COUNT          519 a          940 c          911 g          605 t
ORIGIN      
        1 cgggccgccg cgccgggcag atcgccgcca ccagggccgc cgccattctc agatatgccg
       61 gcttcgagga tgttcgactg ctggacggcg gctacgacca ctgggtgagg tcaggtcttg
      121 gcctggagac cgagcgtcgg gttcccacac cgatcgacga attcggcgtg gcgattccac
      181 gacgtcccga gctgatcatc gatatcgagg acgccaaatc gatcctcgcc gatccggccg
      241 gtgccgccct ggtcagtgtc cgcacctgga aagagcacat cggcaaggtg agcggataca
      301 actacatcga gcccaaaggt cggatctccg gtgatgtctg gggcaactgc gggaccgacg
      361 cttatcacat gcagcactac cgcaacatcg acaacacgat gcgggcatat ccggagatcg
      421 cggcgaactg gaacgaggcc gggatcaccc cggacaagtg ggtcgccttc tactgcggaa
      481 ccgggtggag ggctagcgag acctggttct acgcctacct gatgggttgg gaccagatcg
      541 cggtctacga cggtggctgg ttcgaatgga gccaggaccc ggtcagcaat ccgatcgagg
      601 tcggcgagcc ggaggacgaa gccgccgcgt gagcccaggc gcgacctagc cccagccggc
      661 ctgctcaggt ccaccggtcg gccgagttcg aacatccgca acgagttcaa tggcaatcga
      721 tcggacgcgt atcaggcggc gctcgtgcaa actctgattg gtattggcga ggggggcgac
      781 gtgactcagg agaccgattt tgatctgagg gattacgtct acatcatcgc cctcgcaccc
      841 gcgatcctcg ggacacatgc attcgccgtg cgtctgagcc gcaccgtgga actgaccggg
      901 cgactccaag gccgcagcac ggtcaacctc ctgtccggcg gcctggccct gttgaatctg
      961 ttcgccacgg ctgccatctg tcgactgagc ggcatccccc ttggcatgga agaaggggcg
     1021 ggcgtgatcg acgtcatatt ggccgctttg atcgcgttgg cagtcggatt cgtcctgtgc
     1081 tcagtggcgg cgatgactgt ccgtttcctc cccgatcgac gacgcaccgg accaaaccga
     1141 cgagcaaaca gcttcctcgc attgctcaca accgcgacga aggtcctgtt gtccttggtt
     1201 gtggtcctcc tcgcgatctc gctgatcggc gcctatatcg aacagttcga actcgggttc
     1261 ggagcgctgg gaccgatgga ttgggcgggg ctgttcctgg ctccggtcct gctagtggcc
     1321 ttggccctct atatccgccg tggccaacgg aaggcgacgt cctacgccaa gcgactccgt
     1381 ccatcatccg aggactcagt cgagggcgaa ccgcgtgtgc tgtatctgcg acccttccat
     1441 gaggaacatc ggttgttcgc cggatcccag accctcgacg aattcctggg agacgagatc
     1501 gccaaacaga tcggtccgct gatcgccctg ggcaacccga ctgacaggat ctcgccggat
     1561 ggggccgagc ggcagtacta ccaggatgat gattggcaat cagcggtgga ggcgctggcc
     1621 ctcgaagctc catgcatcgt tgctgccacc agcgcatcgc ccaacacagc atgggagctg
     1681 cgtcgggtgc tggagcttgg attggagcga cgtatctatc tgctttcccc tcccccacga
     1741 gaggccgaga ccccacccac ggcatcggat gaagaggctg gtcctatccg ccgtgccctc
     1801 cggatcgcgc gccgcgccac cttttcctgg ctggcccagg acttggacgg gctgtcacgc
     1861 gaggtctctc tcggtcagct cccctcggct ttgtcaccag ccggcatgac cccctggcac
     1921 gagctggtcg agacgctcgc cgcgtgcggt tactcagtgc atttctccga ccctggtcct
     1981 ggaacggtga ttggattcga gaccggtggt atggctgtca tcctcgagca gggtgcgacg
     2041 tcgccgattg actacatcaa gccgattgtc gatcgctccg tgcgtcccca gccgcctggc
     2101 cctcagccct gatcctggcg ggccggggct tcccggcgat ggcctgcccg tcatccaaat
     2161 ctctcctacc gtggacttcg accgacaccg aatggaccgg attacgacta cgtggacaac
     2221 acccctccta cctatggcct gtggggcttg gttcgatact ttctctacct tggcactgcc
     2281 ggattcggtg gccccgtcgc tttggtcgaa tacatgcacc gggatttggt cgagggtcgg
     2341 cgctggatca cagaggacga atatcgggaa gggctagccc tcgccaacgt ctccccgggg
     2401 cctttggccg cccagttggc gatatatctc ggatacgtcc attactcctt cctcggagca
     2461 acgttggtgg cggtcgcatt cgtgctcccg tccttcttga tggtcgtcgc catcggatgg
     2521 gcctacctcg aatacgccgg actcccgtgg atgcaggcgg tcttctatgg cgtggctgcg
     2581 gcaatcatcg gaatcatcgc ttggagcgca tatcggctca ccagcaagac catctcgtcg
     2641 gacctactgc tgtgggtgat cctcgtgtcg atggcagcaa tcaccgcgat caccgaaacc
     2701 gagcacatca tcctgttccc gatcgcgggg ctggctgtct ggattgcacg gaatccacga
     2761 attcgtcgcc gggacacggt gaatggtgtt ctcctacttc cattagccct gcaatcagct
     2821 gtggccgatc cgtcagcggg tgagatcttc tggttcttct tcaaagcggg agcgtttgtg
     2881 ttcggtagtg gcctggccat cgtgccattc ctctacggcg gtgtggtcgt cgaatacgga
     2941 tggctcgact cacaggagtt tctcgacgct gtcgc
//
LOCUS       DASTXO010000051         2880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1550428, whole genome shotgun sequence.
ACCESSION   DASTXO010000051 DASTXO010000000
VERSION     DASTXO010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550428"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1296
                     /locus_tag="VFU96_01030"
     CDS             <1..1296
                     /locus_tag="VFU96_01030"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEU5111865.1"
                     /translation="TSLVLAQPVKNNLYMAAPPDERPPFWRRKKWARRLLGEFDLDME
                     LFPDSPAGFLTLAERQMFEVAKALVSDPKVLLLDEPTTALGPDEVEALHRTVAACRRR
                     GVGVVYVSHRLPEVLEIADRITVLRDGKIRGTFDAHTTSEHELVELIVGRPFESAFPA
                     AQMKGERREVLVVEGFQGQSFGPVSFTLEAGEVVGIAGAEGNGQPQLFDTLAGRTPPR
                     AGRVVCDGKELTLISTHEAVRSGIMLLPGDRKREALAPVLGVRANSTIQSLRRFSMFG
                     FLRRRAERKAVKDLVSELEIRTPSLEQPVEFLSGGNQQKVSVSRTFLRKPSVILAYEP
                     TQGVDVGSRFDIYRALRARTDAGAALLVKSSDPIELSGLCDRVLVMSRGTIVEEIPGD
                     ELDELRIVEAIVRGPGVSRSGRSPFGVAMPKAPTQQPTS"
     gene            1293..>2880
                     /locus_tag="VFU96_01035"
     CDS             1293..>2880
                     /locus_tag="VFU96_01035"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111866.1"
                     /translation="MTSLVERSRQEVRKVLRNARDKNKWRKVIKVRLWMPVALQVLLI
                     VGVVWFTDSRFEGFINRTNTVNILLIAIPLAVATMAQIHALLVGYLDLSIGAMIGLSV
                     VIASFMIQSESSPAELLAGLGTILLCALAVGLFNAALIRGVKIPSIIATLATLSILDG
                     ISLTLRPTPGGTIDPAFLALLRGGIGPVPIAFLLVVVGAGLLDIWLHASGSGLKVSSV
                     GFDERSAKRSGVRTNWVRVRALMLSALFAAVAALFVMTRSGVGNAQIGTSYTLNSITA
                     AVLGGAALAGGRATFVGGVVSSVLLAVIITVLPFHGLPPDVGPVVIGVLVLIGIILFQ
                     VGDIKELVKRNYRRARRIVLGSRPPKAAELPALYPTGNDFSVVPTGRKLIKGGIVLSL
                     DPAVGDFARGDVLIDGDRIVKVGPNLANGEMEIIDAAGMIVMPGFVDSHRHIWEGILR
                     NIGTDVPLEGRVSYISFVLRTLAPVFRPEDAYAGNLVSSLGAIDAGITTLLDWSHIQA
                     SPAHTDAVVQALEDSGLRAVF"
BASE COUNT          451 a          943 c          941 g          545 t
ORIGIN      
        1 acctcgctgg tcctggctca accggtgaag aacaatctct atatggcggc gcccccggac
       61 gagaggccac cgttctggag gcgaaagaag tgggcgcggc gcctcctcgg cgagttcgac
      121 ctcgacatgg agctgttccc cgattctccg gcgggtttcc tcaccctggc cgagcgccag
      181 atgttcgagg tggcgaaggc gctggtctcc gatccgaaag tgctactcct agacgaaccg
      241 acgacggcgc ttggccccga cgaggtggag gcattgcatc ggacggtggc tgcgtgcagg
      301 aggcgcggtg tgggcgtggt ctatgtgagt catcgtctcc cggaggttct ggagatcgcc
      361 gaccggatca cggtgcttcg cgacggcaag atacgtggca ccttcgacgc ccataccacc
      421 agcgagcatg agctcgtcga gctgatcgtt gggcgtccat tcgagagcgc attccccgcg
      481 gcccagatga aaggggagcg ccgtgaggtg ctcgtggtcg aaggtttcca gggccagtcc
      541 ttcggcccgg tcagcttcac cctggaggcg ggcgaggtgg tggggatcgc cggcgccgag
      601 ggaaacggcc agccccagct cttcgacacg ctcgccgggc ggacccctcc ccgggccggg
      661 cgtgtggtct gtgacggcaa ggagctcact ctgatctcga cgcacgaagc cgtccggtcc
      721 gggatcatgc tgctgcccgg cgatcgcaag cgcgaggccc tggccccggt gctcggggtt
      781 cgggccaatt cgacgatcca gtccctccga cgtttcagca tgttcgggtt cctgcgccgg
      841 cgtgccgagc gcaaggccgt caaggatctc gtctccgagc tcgagattcg cactccatct
      901 ctcgagcagc ctgtcgagtt cctctccggg ggcaaccagc agaaagtatc cgtgtcccgg
      961 accttcctgc gaaagccctc ggtgatcctc gcctacgagc ccacccaggg cgtggacgtg
     1021 ggctctcgct tcgatatcta ccgggcactg cgggcgagga ccgacgccgg tgccgccctc
     1081 ctcgtcaagt cgagtgaccc catcgagctc tccggtcttt gcgatcgggt cctggtgatg
     1141 tcccgcggga ccatcgtcga ggagataccc ggcgacgagc tcgacgagct tcgcatcgtc
     1201 gaggcgatcg tccgcggtcc cggcgtgtcc cgctccggac gatcgccatt cggagtggcc
     1261 atgcccaagg ccccgaccca gcaacccacc tcgtgaccag cctggtcgaa aggtcccggc
     1321 aggaggtccg caaggttctc cgcaacgccc gggacaagaa caaatggcgc aaggtgatca
     1381 aggtcaggct ctggatgccc gtcgcgctcc aggtcctgtt gatcgtggga gtggtctggt
     1441 tcaccgattc gagattcgag gggttcatca accggaccaa cacggtcaac atcttgctca
     1501 tcgccattcc cctggcagtg gcgacgatgg cgcagattca cgcgctcctc gttggctatc
     1561 tggacctttc cattggtgcc atgatcggac tctcggtcgt catcgcctct ttcatgatcc
     1621 aatccgagtc atcgccggcc gaacttctcg ccgggctcgg caccatcctt ctctgcgccc
     1681 tggcggtggg ccttttcaat gcggctctga tccggggagt gaagatcccc tcgatcatcg
     1741 ccacgttggc cacgttgagc atcctcgacg ggatctcgct cacgctgcgc ccgacccccg
     1801 gagggacgat cgacccggcg ttcctggcac tgctccgtgg aggaatcggg ccggtcccga
     1861 ttgccttcct cctggtggtg gtgggagcgg gactgctcga catctggctt catgcgagcg
     1921 ggtccgggct caaggtcagc tcggtcggat tcgacgagcg gtcggcgaag cgaagcgggg
     1981 tcaggaccaa ctgggtgagg gtgagagccc tgatgctctc cgcgttgttc gccgcggtgg
     2041 ccgcattgtt cgtgatgacc cgttccgggg tgggcaatgc ccagatcggc accagctaca
     2101 cgctgaacag catcaccgcc gcggtcctcg gcggggcggc cctcgccggc gggcgcgcca
     2161 ccttcgtggg cggagtggtc tcctcggtcc tcctcgccgt gatcatcacc gtgctcccgt
     2221 ttcatggcct tcccccggac gtcgggccgg tagtgatcgg tgtcctggtg ttgatcggga
     2281 tcatcttgtt ccaggtcgga gacatcaaag agctggtgaa gcgcaattac cgccgagcga
     2341 ggcggatcgt cctcgggagc aggcccccaa aggcggccga gctgccggcg ctctacccca
     2401 cgggcaacga cttcagcgtg gtcccgaccg ggcgcaagtt gatcaaaggt gggatcgtcc
     2461 tcagcctgga cccggcggtc ggtgacttcg cccgcggtga cgttctcatc gatggagatc
     2521 gcatcgtgaa ggtcggtccc aacctggcca acggtgagat ggagatcatc gatgcggccg
     2581 ggatgatcgt catgcccggg ttcgtcgaca gccatcgtca tatctgggag gggatcctgc
     2641 gcaacatcgg caccgacgtc cccctggagg ggagggtcag ctacatctcc ttcgtcctgc
     2701 gcaccctggc gccggtattc cgaccggagg acgcctatgc gggcaacctg gtctcctcgc
     2761 tcggggccat cgatgccggg atcaccacgc ttctcgattg gtcccatatc caggcgtcac
     2821 cggctcacac cgacgccgtc gtccaggccc tcgaggactc cggtctgcgg gcggtgtttg
//
LOCUS       DASTXO010000052         2244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1218837, whole genome shotgun sequence.
ACCESSION   DASTXO010000052 DASTXO010000000
VERSION     DASTXO010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218837"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..1044
                     /locus_tag="VFU96_01040"
     CDS             244..1044
                     /locus_tag="VFU96_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013806792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HEU5111867.1"
                     /translation="MSLEVGDPALLIDSKGRRFLLELTPGRTFQYHEGAVSHADIIGR
                     EEGSWIVSSTGAHLLLLRPRLADFTLKMKRSAQVVYPKDLGPILVYADIAPGMTVLEA
                     GSGSGALTLGLSRAVGPTGMVVSVELREDHAAQARKAIEKWYGRVPENVDLRIGDVAE
                     VVESVRPPRIVLDLPEPWSVVTSAAEHQPPGGLLAAYLPTVPQVQTTVEAARDTGVFT
                     EIEVKEFLVRDWNVAGRSVRPEHQMIGHTGFLVFMRKTADRPTTPVES"
     gene            1206..>2244
                     /locus_tag="VFU96_01045"
     CDS             1206..>2244
                     /locus_tag="VFU96_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5111868.1"
                     /translation="MDDGRRADDATQLRSVVAALEEEIAVLRRRVRDAPARVEDLENE
                     LSRMRERLDRAMTQNDKLSAVLEEAREQLGVLREEVEKLTSPPNNFGTVLQVNDDGTL
                     DIMTGSRKLRVAAQPNVEVKKLQVGQEVLLNEAFNIIDIRHFEPAGDVLRVREVLADG
                     RVVVFAHADEERVLQRSETMADVKLRPGDYVRIDVKTGLILEKMERPEVEELVLEEVP
                     DITYEDVGGLADQIEQIRDTVELPYMHKDLFREYELDPPKGILLYGPPGCGKTLIAKA
                     VANSLAKKVSEQTGSPDVRSYFLNIKGPELLNKWVGETERQIRLIFQRAKEKSDEGVP
                     VIVFFDEMDSLF"
BASE COUNT          448 a          682 c          713 g          401 t
ORIGIN      
        1 aagagctggc gctgatcacc ggtgcgtagg ccgcgcagcg tccgatcgag gcttgcagcc
       61 cggatatccc tgatgccaat gagacgtaac agccaatcca tgtgtctacc gttgccgcca
      121 atcaatcgaa ggtcaatcga gttccatcca catgcagaat aagcaattcg tcaaggtcgt
      181 aatttgggtc gtggtcgtct ccatggtcct ggggctgggc ttcacggtgt tcgctctgct
      241 cacatgagct tggaggtggg cgatcctgcc ctcttgatcg actccaaagg gcgtcgattc
      301 ctccttgaat tgacccctgg ccgcaccttc cagtaccacg agggcgccgt ttcccacgcc
      361 gacatcatcg gacgggagga gggcagctgg atcgtctcgt cgactggggc ccatcttctc
      421 ctcctccgcc ctcggttggc agatttcacg ctcaagatga agcggagcgc ccaagtcgtc
      481 tatcccaagg atctcggccc gatcctggtg tatgccgaca tcgcacccgg tatgaccgtc
      541 ctcgaggcgg ggagcgggtc tggggccctc accctgggcc tgagccgcgc cgtcggaccc
      601 accgggatgg tggtcagtgt cgagctcagg gaggatcacg ccgctcaggc cagaaaggcc
      661 atagagaagt ggtatggacg agtgcctgag aacgtggacc tacggatcgg tgacgtggcc
      721 gaagtggtcg agtcggtccg accgccccgg atcgttctcg acctgcctga gccctggagc
      781 gtcgtgacct ctgccgccga gcatcagcca ccgggtgggt tgctcgccgc ctacctccca
      841 accgtccccc aggtgcagac cacggtggag gccgctcgag acacaggcgt cttcaccgag
      901 atcgaggtca aggagttcct ggtgcgggac tggaatgtcg cggggaggtc cgtgcggccc
      961 gagcatcaga tgatcgggca caccggcttt ctcgtcttca tgaggaagac cgccgacagg
     1021 cccaccaccc cagtcgagag ttgatcggtc acaaccctca agtcgcggcg tcgtcacgcc
     1081 gacgctcttc tttgtcggcg atagctggca tcatcgagcg ggtagggctc cggtgacaac
     1141 ccggggaact acagagaccg attcgtatac tgctaaacaa ggcgaccaag gccaggagga
     1201 agcccgtgga cgatggacgg agagcggacg acgccactca gttgcggagt gttgtcgctg
     1261 ccctcgaaga ggagatcgcc gtcctgcgac gtcgcgtgcg tgacgcaccg gctcgcgtcg
     1321 aggacctcga gaacgagctc agcaggatgc gggagcgcct cgatcgggcg atgacccaga
     1381 acgacaaact gtcggcggtc ctcgaagagg cgagagagca gctcggtgtc ctccgggagg
     1441 aggtcgagaa gctgacctca cctccgaaca acttcggcac ggttctccag gtgaacgacg
     1501 atggcaccct ggacatcatg accgggagcc gaaagctgcg ggtcgccgcc cagcccaacg
     1561 tcgaggtgaa gaagctccaa gtcggtcagg aagtcctcct caacgaggcg ttcaacatca
     1621 tcgacatccg tcacttcgag ccggccggtg acgtactgcg ggtgcgcgag gtgctcgccg
     1681 acggccgggt tgtggttttc gcccacgccg acgaggagcg agtccttcag aggtcggaga
     1741 ccatggccga cgtcaagctc aggcccggtg actacgtccg catcgatgtc aagacgggtc
     1801 tcatcctgga gaagatggag cgacccgagg tcgaagagct cgtcctggaa gaggtgccgg
     1861 acatcaccta tgaggacgtc ggaggtctag ccgaccagat cgagcagatc cgtgacaccg
     1921 tagagctgcc gtacatgcac aaggatctgt tccgggagta cgagctcgat ccccccaagg
     1981 gcatcctgct ctatggccct cccggttgcg ggaagacgct catcgccaag gccgtggcca
     2041 acagcctcgc caagaaagtg tcagagcaga ccggcagccc cgacgttcgc tcctatttct
     2101 tgaacatcaa gggcccggag ctgctcaaca agtgggttgg tgagacggag cgtcagatcc
     2161 ggctgatatt ccagagagcc aaggagaagt cggatgaagg ggtgcctgtc atcgtcttct
     2221 tcgatgaaat ggactctctg ttcc
//
LOCUS       DASTXO010000053         2678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1034689, whole genome shotgun sequence.
ACCESSION   DASTXO010000053 DASTXO010000000
VERSION     DASTXO010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034689"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="VFU96_01050"
     CDS             <1..1143
                     /locus_tag="VFU96_01050"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: protein motif:HMM:NF010372.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HEU5111869.1"
                     /translation="VGDDERLEIADWVEIVPRTPLSGDTTATFEVEDPHRVTHVRLNI
                     FPDGGVARLRIEGDPIPGRDEVCPEDDAIDLASSLVGGEAVDASDVHYSPPSNLLRPT
                     EPAGMWDGWETKRRRGPGNDWAVFRLGLPGVIDSLEVDTRHFKGNAPGWVSVAVSADG
                     ESWEEVVDRAKMKPDAVNPVTLDRPARAGSVRLDIHPDGGVARLRVLGRPDPNHAERL
                     RLLYLNALFDEEAHRFFHGACASTRWVDGMISSRPFADGAAVMTAAAAAFDSLSDGDW
                     FEAFAGHPRIGERGDLQANQEQSGTANAEASLLAELGAANQAYEAAHGFTYIVYASGK
                     TAQEMLDIARGRIGNTREEELVNAAAEQRMITETRLRRMLCLGEPG"
     gene            1140..1457
                     /gene="uraH"
                     /locus_tag="VFU96_01055"
     CDS             1140..1457
                     /gene="uraH"
                     /locus_tag="VFU96_01055"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006367451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEU5111870.1"
                     /translation="MTGLSTHVLDTVSGLPAAGMQVTLERSSDGGWQHVGEGVTDDDG
                     RIPMLGVALGSGIYRLVFATGDAGNPFFPEVHVIVDLDDARDHYHVPLLLSAYGYTTY
                     RGS"
     gene            1459..2286
                     /gene="pucL"
                     /locus_tag="VFU96_01060"
     CDS             1459..2286
                     /gene="pucL"
                     /locus_tag="VFU96_01060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004846 - urate oxidase activity [Evidence
                     IEA];
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urate oxidase"
                     /protein_id="HEU5111871.1"
                     /translation="MPRLGKNRWGKTDVRISKIHRTDSGDDFSDLNVQILLEGEVEAA
                     HTMGDNKGVLPTDTMKNTVYGLAQGHLTQDLEAFAAVLGDHFLAREGVTGATVSISQR
                     LWARAGANGFIGGGSERRTARVARGTSGAGGDRTWAGIEGLVVLKTTGSAFSGFPRDE
                     FTTLPETDERILATSVTATWRYSVMPEDTTETWERAREALLERFFGAWSASVQHQGWL
                     MAVGLLETIPEIEEVEFHLPNQHHLAYDISRFGMEYEGTVFQPVSEPYGDIGFIVTR"
     gene            complement(2301..>2678)
                     /locus_tag="VFU96_01065"
     CDS             complement(2301..>2678)
                     /locus_tag="VFU96_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014400187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate kinase"
                     /protein_id="HEU5111872.1"
                     /translation="VHHAMRLHYDHIRHSLTHAYYQGWDLHPAQFPTRYAAVYAFFLE
                     GLDQAGDRLSNFVESAAKATLVGEVFDDAATGQGLLNFFLRAINCGAIDETEATRRTG
                     LTLDEIQTRSFLAILEGRTTPTT"
BASE COUNT          474 a          822 c          922 g          460 t
ORIGIN      
        1 gtcggtgacg acgagcgcct ggagatcgcg gattgggtcg agatcgtccc tcgcaccccc
       61 ctgtccggag acactacggc caccttcgag gtcgaagacc cacaccgggt cacccatgtt
      121 cgactgaaca tcttccccga cgggggggtg gcgcgcctga gaatcgaggg cgatccaatc
      181 ccgggcagag atgaggtctg tcctgaggac gatgcgatcg acctggcttc gtcactggtc
      241 gggggagagg ctgtcgacgc ttccgatgtc cattactcgc ctccttcgaa cctccttcgg
      301 ccaaccgagc ctgccgggat gtgggacggc tgggagacca agcgtcggcg tgggcccggg
      361 aacgactggg cggtcttccg tctcggattg ccgggcgtga tcgactctct cgaggtggac
      421 acgcgccatt tcaagggcaa cgcgcccggc tgggtctcgg tcgcggtgtc ggctgatggc
      481 gagagctggg aagaggtggt cgaccgcgcg aagatgaagc cggacgcggt caacccggtg
      541 acactcgatc ggccggctcg agccggatcg gtgcgtctcg acatccaccc cgacggtggc
      601 gtggcccgac ttcgcgtgtt gggccggcca gatccgaatc atgccgagcg gctgcgtttg
      661 ctctacctga acgccctgtt cgacgaggag gcccaccggt tcttccacgg cgcctgcgca
      721 tcgactaggt gggtcgacgg gatgatttcg agccgaccat tcgctgacgg ggcggcagtg
      781 atgaccgcgg cggcggccgc tttcgactca ctgagcgacg gcgactggtt cgaggcattc
      841 gccggtcacc cccgcatcgg cgagagggga gacctccagg ccaatcagga gcagagcggc
      901 accgccaacg cggaggcgtc gttgctcgcc gagctcgggg cggcaaacca ggcgtatgaa
      961 gcagcccacg gattcaccta catcgtctat gcgtccggca agacggctca ggagatgctc
     1021 gacatcgccc gcggccgcat tggcaacacc cgtgaggagg agctcgtcaa tgccgcggct
     1081 gaacaacgga tgatcacgga gaccagactg cgccgcatgc tctgtctcgg agaacccgga
     1141 tgaccgggct cagcacacat gtgctcgaca ccgtctctgg cctgccggca gccggaatgc
     1201 aggtgacgct ggagcgctcg tccgacgggg gctggcagca tgtcggcgag ggtgtgaccg
     1261 atgatgacgg acggataccc atgctcggag tcgcgctcgg ttcagggatc taccgcctcg
     1321 tgttcgccac cggcgacgca ggcaacccct tcttcccgga ggtgcatgtg atcgtcgacc
     1381 tcgacgacgc tcgggatcac tatcacgtcc cgttgctgct cagcgcctac ggatacacga
     1441 cctaccgggg tagctgagat gccccgactc ggaaaaaacc gttggggcaa gaccgatgtt
     1501 cgaatctcca agatccaccg caccgattcg ggcgacgatt tctccgatct gaatgtccag
     1561 atcctgctcg agggggaggt cgaggcggcg cacacgatgg gcgacaacaa gggtgtgctc
     1621 cccaccgaca cgatgaagaa tacggtctac ggtctggcgc aagggcatct gactcaggac
     1681 ctggaggcat tcgccgccgt cctgggcgat catttcctgg cgcgtgaggg tgtgaccggc
     1741 gccactgttt cgatctcaca gcggctctgg gcccgagccg gggccaacgg gttcataggc
     1801 ggtgggtcgg agcggcggac ggcacgcgtg gctcgcggca cgtcgggggc gggcggcgac
     1861 cgcacctggg cagggatcga gggcctggtc gtcctcaaga ccaccgggtc tgccttttcc
     1921 ggattccccc gggacgagtt caccaccctg cccgagaccg acgaaaggat cctggcaacc
     1981 tcggtgacgg ccacctggcg atactccgtc atgccggagg acacaaccga gacctgggag
     2041 agggcccgcg aagctctgct ggaacgcttc ttcggtgcct ggtcggcgtc cgtgcagcac
     2101 cagggctggc tgatggccgt gggcctcctc gagacgatcc ccgagatcga ggaggtcgag
     2161 ttccatctcc ccaaccagca tcatctggcg tacgacatct cccgattcgg gatggagtac
     2221 gaggggacgg tgttccagcc ggtgtccgag ccctacggcg acatcggttt catcgtcact
     2281 cgctgatcag gaggcggaga tcaggtggtg ggggtggtgc ggccttccag gatggcgagg
     2341 aaggagcgag tctggatctc atcgagggtc agcccggttc gcctggtcgc ttccgtctcg
     2401 tcgatcgcgc cgcaattgat cgctcgtagg aagaagttga gtagaccctg gccggtcgcg
     2461 gcatcatcga acacttcacc gacgagggtg gcctttgccg ccgactcgac gaagttggaa
     2521 agacgatcgc cggcctggtc caggccctcg agaaagaatg cgtagaccgc ggcataacga
     2581 gtggggaact gggcggggtg gaggtcccag ccctggtagt aggcgtgggt cagagaatgc
     2641 ctgatgtggt cgtagtggag ccgcatcgcg tggtggac
//
LOCUS       DASTXO010000054         3767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1292560, whole genome shotgun sequence.
ACCESSION   DASTXO010000054 DASTXO010000000
VERSION     DASTXO010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292560"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="VFU96_01070"
     CDS             <1..564
                     /locus_tag="VFU96_01070"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEU5111873.1"
                     /translation="LIVLARPLVSLLYEHGEFSAADGSLVARLLVLYAFSIPAWGLHQ
                     ILSRHFYAQRMMWRVVLIGTVGTLVAIPVWLGLYSAMGVEGFALASTLVMTAYALALL
                     VAWGVDAGWSPVRALVPSILRGLVAAALAAAVAYPIVSLMFGADYADLWEAVAAAALG
                     GLTTIAVFLGVSYLLRAPELGDLMRRG"
     gene            complement(571..771)
                     /locus_tag="VFU96_01075"
     CDS             complement(571..771)
                     /locus_tag="VFU96_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111874.1"
                     /translation="MIRDIEIRGGAGPFEAALIVVVLDRISEEERAASQAPKDGSRPL
                     PMWMLAERPEEPNMPRDILRPR"
     gene            complement(768..1175)
                     /locus_tag="VFU96_01080"
     CDS             complement(768..1175)
                     /locus_tag="VFU96_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5111875.1"
                     /translation="MKLINLDHVAIAVHDLDASLADYRSRYRVEPLYREIVEAQGVEE
                     AMIPVGGSFVQLLQPLAVETPVGRFLESRGEGLHHVAFAVASIDIALEHLREEGARLV
                     DEAARPGGRGTRIGFVHPADLAGTLIELVELPA"
     gene            1244..2425
                     /locus_tag="VFU96_01085"
     CDS             1244..2425
                     /locus_tag="VFU96_01085"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005440330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEU5111876.1"
                     /translation="MSNPVITGSARTPIGKYGGSLAPLHAVELGGTAIAAALTRGGVD
                     PGAIDEVLFGHVLQAGEGQITARQAAVRGGLPMTVPSVTINKVCLSGMSAIGLAERGI
                     RAGDSKFVVAGGMESMSNAPHVVRDLRWGVRIGNAEMIDVMQHDGLFCAFDQCTMGES
                     SDRKNQRLGIGREEQDAWAARSHQRAVEATRNGTMAREIAPVSVPKAKSEPTIVDTDE
                     GIREGSTQESLSALRPAFAPGGTITAGNASQISDGAAAVVVADRDAAEASGAPILAEI
                     LAYGQIGGGDATLHERPAEALLVALKRAGLDVGDLDIVEINEAFAAVAFWSARMLDIP
                     DSKVNVNGGAIALGHPLGATGARLVVSLINALYARGGGIGAATLCGGGGQGDAIIVKV
                     S"
     gene            2425..3417
                     /gene="glpX"
                     /locus_tag="VFU96_01090"
     CDS             2425..3417
                     /gene="glpX"
                     /locus_tag="VFU96_01090"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEU5111877.1"
                     /translation="MPEPVERNLGLDLVRVTETAAMGAARWLGRGNKEAADQAAVDGM
                     RLHLSTLDIDGLVVIGEGEKDSAPMLYNGERVGTGDGLVVDVAVDPVDGTTLTASGMP
                     GAIAVIALAEGRGTMYAPGSLVYMDKIAVGPEAAGVVELEAPVEHNLRQVAKAKRKEV
                     NDLRVLILDRERNKHYIDGVRQAGARITLIRDGDVAGAIATAQDDTAADILLGIGGSP
                     EAVIAAAALKCMGAEIQCKLWPRNDQERRLAEDEGLDLDEVIGTSQLVSSDNVFFSAT
                     GVTGGEYLRAVEFRREDHVVTHSVIMRSKTGSIRYMEATHNIARLREISARTLD"
     gene            complement(3425..>3767)
                     /locus_tag="VFU96_01095"
     CDS             complement(3425..>3767)
                     /locus_tag="VFU96_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111878.1"
                     /translation="RFMRRWLHVGDVVDENAILGARRSTTAAIVVSGIRCIITYLLIP
                     VLAPLIGISDAASAPVTIALSVLAVLMGITGVRRFWMADHRARWAYTTFIAVIVALLI
                     VGIVLDVASMI"
BASE COUNT          668 a         1227 c         1257 g          615 t
ORIGIN      
        1 ctgatcgttc tcgctcgtcc cctcgtcagc ctgctctatg agcacggcga gttctccgcc
       61 gctgatggca gcctcgtcgc ccgtctcctt gtcctctacg cattctccat ccccgcctgg
      121 ggtctccacc agatcctctc ccggcacttc tatgcccaaa ggatgatgtg gcgggtggtg
      181 ctgatcggca ccgtgggcac ccttgtcgcc atcccggttt ggctcgggct ctactcggcg
      241 atgggcgtcg aggggtttgc cctggcctcc acgctggtga tgaccgcata cgccctggcc
      301 ttgctcgtcg cctggggggt cgacgcgggg tggtcgccgg tgcgagccct ggttccgtcg
      361 attctccggg gcctggtcgc tgccgcgctc gcggccgcgg tggcctaccc gatcgtgtct
      421 ctgatgtttg gcgccgatta cgcagacctg tgggaggccg tggccgccgc cgctctgggt
      481 ggcctcacca ccatcgcagt gttcctcggg gtctcctacc tgctccgagc ccccgagctc
      541 ggcgacctga tgcggcgcgg ctgactcaga tcaacgaggg cggaggatgt cacggggcat
      601 gttcggctcc tcgggtcgct ccgcaagcat ccacatagga agagggcgtg atccatcctt
      661 cggcgcctgg ctggcggccc tctcctcctc gctgatgcgg tcgaggacga cgacgatcaa
      721 cgccgcctcg aacggcccgg cgcccccccg gatctcgata tccctgatca tgcgggcaac
      781 tccacgagct cgatcaaggt accagcgagg tctgcgggat ggacgaagcc gatgcgggtg
      841 ccccgtccac cgggacgggc ggcctcgtcg acaagcctcg ctccctcttc ccgcaaatgc
      901 tccagggcga tgtcgatcga ggcaaccgca aaggcaacgt ggtgcaatcc ctcacctctg
      961 ctctcgagga agcgcccgac cggtgtctcg acggcgagag gctgcagaag ttggacgaag
     1021 gatcctccca ccgggatcat cgcctcttcg acaccttggg cctccacgat ttcccgatac
     1081 agcggctcga cccggtaacg actgcggtaa tccgcgagcg aagcatccag gtcatgcacc
     1141 gcgatcgcca cgtggtcgag attgatcagc ttcaccggcg cgaggctaaa gcaccgggtt
     1201 gtcagagagg gcagacaccc gcggccggta gcatccaccg ccagtgagca acccagtcat
     1261 caccggctcg gcgcgaaccc caatcgggaa gtatggcggc tccctcgctc ccctccacgc
     1321 cgtcgaactc gggggaacgg ctatcgcagc tgccctgacg cgcggcggag tcgacccggg
     1381 cgcaatcgac gaggtgttgt tcgggcatgt gctccaagcg ggcgaagggc agataaccgc
     1441 ccgtcaggcc gccgtgcggg gtggtctccc tatgactgtc ccgtcggtca ccatcaacaa
     1501 ggtgtgtctc tccgggatgt cggccatcgg cctcgccgag cgggggatca gggccggaga
     1561 ttccaagttc gtggtggccg gtggcatgga gagcatgtcc aacgccccac acgtggtccg
     1621 cgatctgagg tggggagtac ggatcggcaa cgccgagatg atcgacgtca tgcaacacga
     1681 tggcctgttt tgcgccttcg accaatgcac catgggtgag tcctccgatc gaaagaacca
     1741 gcgactcggc atcggtcggg aggagcagga cgcttgggcc gcccggagcc atcagcgtgc
     1801 cgtggaagcc actcgcaacg gcacgatggc tcgtgagatc gcgcctgtgt cggtccccaa
     1861 ggccaagagt gagcccacca tcgtcgacac agatgaaggg atcagggagg gatcgacgca
     1921 ggaatcgttg tccgcactac ggcctgcctt cgcgcccggg ggcacgatca ccgccgggaa
     1981 cgcctctcag atctccgacg gggcggccgc cgtggtcgtg gcagaccgcg acgccgcgga
     2041 agcatcgggg gccccgatcc tcgccgagat cctcgcctac ggtcagatag gtggtggaga
     2101 cgccactctc cacgaacgac cggctgaggc ccttctggtc gcgctgaagc gagctggcct
     2161 ggacgtcggc gacctcgaca tcgtggagat caacgaggcg ttcgccgccg tcgcattctg
     2221 gtcggcccgg atgctcgaca tccccgacag caaggtcaac gtcaacgggg gcgccatcgc
     2281 gctcggtcat ccacttggag ccaccggtgc caggctcgtg gtcagcttga tcaatgccct
     2341 ttacgcccgt ggcgggggaa tcggggccgc caccttgtgc ggaggcggag gccagggcga
     2401 tgccatcatc gtcaaggtga gttgatgccc gagcctgtgg agcggaacct gggcctcgac
     2461 ctggtgagag tcaccgagac cgccgccatg ggagcggcac gatggctggg gcgaggcaac
     2521 aaggaagccg ccgaccaggc cgcggtcgat gggatgcgac tgcacctatc cacactcgac
     2581 atcgacgggc tcgtggtgat cggtgaggga gagaaagact cggcgccaat gctctacaac
     2641 ggcgagcgag tgggaacagg cgatgggctg gtggtcgacg tggccgtcga tccggtcgac
     2701 gggaccacgc tcacggcttc gggcatgcct ggtgccatcg cggtgatcgc cctcgccgag
     2761 ggccggggca cgatgtatgc ccccggctca ctggtctaca tggacaagat cgccgtcggt
     2821 cccgaggcgg ctggggtagt cgagttggag gccccggtcg agcacaacct ccgtcaggtg
     2881 gccaaggcca agcggaagga agtcaacgat ctccgggtcc tgatcctcga ccgggaacgc
     2941 aacaagcatt acatcgacgg ggtgaggcag gcgggagccc ggatcacgct catcagggac
     3001 ggcgacgtgg ccggagcaat cgccactgcc caggacgaca ccgccgccga catcctgctc
     3061 ggcatcggag gctcgcccga ggctgtcatc gcggctgcgg ctctgaaatg catgggcgcc
     3121 gagatccagt gcaagctgtg gccgcggaac gaccaggaaa ggcgactagc cgaggatgaa
     3181 ggcctcgatc tcgacgaagt gatcggcacc agtcaactgg tctcatccga caacgtcttc
     3241 ttctcggcaa ccggcgtgac cggtggcgaa tatctgcgcg ccgtcgagtt cagacgcgag
     3301 gaccatgtgg tgacccattc ggtgatcatg cggtccaaga ccggttcgat ccgctacatg
     3361 gaggccaccc acaacatcgc acggctcagg gagatctcgg cccgaaccct cgactagcgg
     3421 tccgtcagat catcgaggcc acatcgagga cgatcccgac gatgagcagg gccacgatca
     3481 cggcgatgaa ggtggtgtag gcccaccggg cacggtgatc ggccatccag aaacgccgga
     3541 caccggtgat gcccatcagg acggcgagca cggaaagggc gatcgtgacc ggcgcgctcg
     3601 ctgcgtccga gatgcctatc aggggggcca ggaccgggat cagcagatag gtgatgatgc
     3661 agcggatccc ggagaccacg atcgcggcag tggtcgagcg tcgagcgccg aggatcgcgt
     3721 tctcgtcgac gacatccccg acgtgcagcc aacggcgcat gaaccgg
//
LOCUS       DASTXO010000055         2400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1071614, whole genome shotgun sequence.
ACCESSION   DASTXO010000055 DASTXO010000000
VERSION     DASTXO010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071614"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VFU96_01100"
     CDS             <1..734
                     /locus_tag="VFU96_01100"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEU5111879.1"
                     /translation="DSQDDSVTAIQAVIEPWTSGPNAFLAAPDSAGCAAYQAVWAHLL
                     RLTFHDEIPEDYWPDGGSRWFTVMGELLERPDDPFWDDIRTETVEDRDLILAQAMIGA
                     HDELSGLLGDDPESWRWGDLHAASFENLTFGQSGIAPVEWLFNRTAPNRVGGSDDIVN
                     AVGFHPPDGYGVDWIPSMRMVIDLSDLTASTSVNSTGQSGHAFHRNYADMLASWTDGI
                     HHPMRWTRGQVEDGAQGTLTLLPDS"
     gene            complement(738..>2400)
                     /gene="aceK"
                     /locus_tag="VFU96_01105"
     CDS             complement(738..>2400)
                     /gene="aceK"
                     /locus_tag="VFU96_01105"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF002804.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HEU5111880.1"
                     /translation="AINSRAPRRFEERDWAGMQTDAVERLRLYTVVVDRVMESVSHML
                     GARAEDPLVWVAVKAVYSGLISDRQDWELAETFYNSVTRRVFRTVGVAEHVEFVDSDF
                     DSPPIPSREPIYLTFDRRESTSGLVREILTAFAHAVPYERLDADADEVASRIDARLSE
                     PGALAAVGKAEVVRTVFYRGQSAYIIGLLYAGSHRIPLIIALSHGDNGIFVDAVLLTE
                     DEASIVFSFTRSYFHVDVARPYDLARFLRRLMPRKRIAEIYIAIGQNKHGKTELYRDL
                     LRHLRSTEERFEPVPGTKGLVMIVFGMPGHDDVFKVIRDTFPPPKRTTRRGVMGKYRL
                     VFQHDRAGRLMDVQDFQHLEFHRSRFSPAVLEELLSEAARTVRIQGDSVIISHVYVER
                     RVVPLDVYVREAARPSAVDAVVDFGQSIKDMASTNIFPGDLLPKNFGVTRHGRVVFYD
                     YDELSALTDINFREMPEARDEIEELGPEPWFPVGDNDVFPEEHQSFLGLAADLRQVFF
                     EHHADLFGIDAWRAIQERIRSGELIEVFPYRQAARLPGTGASRGW"
BASE COUNT          446 a          781 c          759 g          414 t
ORIGIN      
        1 tcgattccca ggatgactcg gtcaccgcga tccaggctgt catcgagccc tggacgtccg
       61 gtcccaacgc cttcctagcg gcaccggatt cggcggggtg cgccgcctac caggcagtgt
      121 gggcccattt gctccggttg accttccatg acgagatccc cgaggactac tggcccgacg
      181 ggggcagccg ttggttcacc gtgatgggcg agttgctgga gagacccgat gacccgttct
      241 gggacgatat ccgcaccgag accgtcgaag accgcgacct catcctggca caggcgatga
      301 tcggcgccca cgacgagctg agtggcctcc tcggcgacga tccggaatca tggcgctggg
      361 gtgacctcca tgccgcctcg ttcgagaacc tcaccttcgg ccagtcaggg attgcccccg
      421 tcgagtggct gttcaaccgg accgctccca atcgggtcgg gggcagcgac gacatcgtca
      481 atgccgtcgg gttccaccct ccggatggct acggggtcga ttggatcccg tcgatgcgga
      541 tggtgatcga cctctccgat ctgactgcat cgacttcggt caattccacc gggcagtcgg
      601 gccatgcgtt ccaccgcaac tacgccgaca tgctcgcctc gtggaccgac ggcatccatc
      661 acccgatgcg ctggaccaga gggcaggtgg aggacggcgc gcagggaacg ctgaccctgc
      721 taccggattc ctgagggtca ccaccctctg gaggcacccg ttccaggaag acgggcagcc
      781 tggcgatagg ggaagacttc gatgagctcg ccggaccgaa tcctttcctg gatggcgcgc
      841 cacgcatcga tcccgaacag gtcggcgtga tgctcgaaaa agacctgacg caggtcggct
      901 gcaagcccga gaaagctctg gtgctcctcg gggaagacat cgttgtcccc caccgggaac
      961 cagggctccg ggcctagctc ctcgatctcg tcgcgcgcct ccggcatctc ccgaaagttg
     1021 atgtcggtca gggcgctcag ctcgtcgtag tcgtagaaca cgacccggcc atggcgggtc
     1081 acaccaaagt tcttggggag cagatcgccc gggaagatgt tggtcgaggc catgtctttg
     1141 atcgactggc caaagtcgac cacagcgtcg accgccgatg ggcgcgccgc ttcccgcacg
     1201 tagacatcga gagggacgac acggcgctcc acatagacat ggctgatgat caccgaatcc
     1261 ccctggatcc gcacggtccg ggccgcctcg gacaacagct cctcgaggac cgccggagag
     1321 aaccgggagc gatggaactc gagatgctgg aagtcctgta cgtccatcag cctccctgcc
     1381 cggtcgtgct ggaagacgag ccggtacttt cccatcactc cccgtcgggt agtccgcttc
     1441 ggcggcggga aggtgtcccg gatcaccttg aagacatcgt cgtggcccgg catgccgaac
     1501 acgatcatca cgagcccctt ggtgcccggc accggctcga accgctcctc ggtagaacgc
     1561 agatgccgga ggaggtcgcg gtacagctcc gtcttcccgt gcttgttctg accgatggcg
     1621 atgtagatct cggcgattcg ctttcgaggc atgagccgac ggagaaagcg agccaggtca
     1681 tacggtcggg cgacgtcgac gtggaaatag gacctggtga agctgaagac gatcgacgcc
     1741 tcgtcttcgg tgaggaggac cgcgtcgacg aagatgccgt tgtcgccatg gctcagggcg
     1801 atgatcagcg ggatccggtg tgatccggcg tacaacagcc cgatgatgta ggccgactgg
     1861 ccccggtaga agacagtgcg gaccacctcc gccttgccga ccgcggcgag ggcacccggc
     1921 tcgctgagcc tggcgtcgat ccggctcgcc acctcgtccg catctgcatc gagacgctcg
     1981 tagggcacgg cgtgggcgaa ggcggtgagg atttctctga ccagcccgga ggtcgactcc
     2041 cgacggtcga acgtgaggta gatcggctca cgagagggga tcggcggact gtcgaaatcg
     2101 gagtcgacga attcgacgtg ctctgccacc ccaacggttc tgaacacacg gcgagtgacc
     2161 gagttgtaga aggtctcggc caactcccag tcctggcgat ccgagatgag gccggagtag
     2221 acagccttta ccgccaccca caccaacggg tcctcagccc tcgccccaag catgtggctc
     2281 accgactcca tgactcgatc gacgacgacc gtgtacaggc ggagcctctc gactgcgtcg
     2341 gtctgcatcc ccgcccaatc ccgctcctcg aaccttcggg gtgcacgaga gttgatcgcc
//
LOCUS       DASTXO010000056         2023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_40428, whole genome shotgun sequence.
ACCESSION   DASTXO010000056 DASTXO010000000
VERSION     DASTXO010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40428"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="VFU96_01110"
     CDS             <1..1184
                     /locus_tag="VFU96_01110"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5111881.1"
                     /translation="VVPESVVIVGSGGAGAAAAEMLRRLGYIGPVTMIGLEPPIDRPN
                     VSKDYLAGTAPEDWMPLRTPDFYERHDITLITGAEVTAIDVEARTVALDDGRSIEYGA
                     LLLATGAEPTRLPTPGGDRAHVHYLRTLEDSRSIIAALEGTDHAVVIGAGFIGLEAAA
                     SLRQREVAVTVVAPESLPLVPIIGERMGRFVQDLHEEHGVEFRLGHTVSEIREREVVL
                     DNGETVSADLVVIGVGVVPNTGLGEAAGLKVENGIVVDDRLRTSDPHIWAAGDVASYP
                     DQNAGRVRIEHWVLAQRQGQAAARNILGHDTPFRDPAFFWSQHYDIPINVVGHAEKWD
                     EEIVRGDPSAHDVMVGYRKDGVIQAVASIYRDVESLRAEHALAHQDRDALDRLFDPED
                     S"
     gene            1400..>2023
                     /locus_tag="VFU96_01115"
     CDS             1400..>2023
                     /locus_tag="VFU96_01115"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEU5111882.1"
                     /translation="MPRLFDFSATDGSDPDLLGGKGAGLARMTSLGLRVPPGFTITTE
                     VCRIAMATGAIPDELWPEVKAAVERLEQSSGRSFGSGPAPLLLSVRSGAKVSMPGMMD
                     TVLNLGINDEVVKALIEWSGDSHFAWDAYRRFVQMYGNVVLGVPEHRFQDVLTELRDS
                     KGVADDSELTAEDLEMATTRFRDIVEQERPGELPEDPLHQLEGAITAV"
BASE COUNT          363 a          655 c          656 g          349 t
ORIGIN      
        1 cggtcgtacc cgaatcagtc gtgatcgtgg gctcaggtgg cgcgggcgcc gccgccgccg
       61 agatgcttcg ccgactcggg tacatcggcc cggtgacgat gatcggcctc gaacctccca
      121 tcgatcgccc caacgtctcc aaggactatc tggcgggaac ggcgcccgag gactggatgc
      181 cgttgcggac cccggatttc tacgagcgtc acgacatcac actgatcaca ggggcggagg
      241 tcaccgccat cgacgtcgag gcgcggaccg tcgccctcga cgatgggcgc agcatcgaat
      301 atggggccct cctcctggcc accggcgccg agccgacccg tctgccgaca cccggcggcg
      361 atcgtgccca tgtccactat ctgcggacac tcgaggacag ccggtcgatc atcgccgcat
      421 tggagggaac agaccatgcg gttgtcatcg gggccgggtt catcggactg gaagcggcgg
      481 cgtctctccg tcagcgagaa gtggctgtca ccgtcgtggc gcccgagtcg ctaccactgg
      541 ttccgatcat cggagagaga atgggacgct tcgtccagga cctccacgaa gaacacggtg
      601 tcgagttccg actgggccac accgtgtcgg agatccgaga acgcgaggtc gtgctcgaca
      661 acggggaaac cgtgtcggcc gatctggtgg tgattggcgt cggcgtcgtc cccaacaccg
      721 gcctgggtga ggccgccggg ctcaaagtgg agaacggcat cgtcgtcgac gatcgtctgc
      781 gcaccagcga tccccacatc tgggctgccg gcgacgttgc ctcctatcca gatcagaacg
      841 cggggcgggt tcggatcgag cactgggtgc ttgcccagcg acagggtcag gccgcggctc
      901 ggaacatcct cgggcacgac actccattca gggacccggc cttcttctgg agccagcact
      961 acgacatacc gatcaatgtc gtcggccatg ccgagaagtg ggacgaggag atcgttcggg
     1021 gggacccgtc cgctcacgac gtgatggtcg gctaccgaaa ggatggtgtc atccaagcgg
     1081 ttgcctcgat ctaccgggac gtggaatctc tgcgtgccga gcatgcactc gcccaccagg
     1141 atcgcgacgc actggaccgg ctcttcgacc ctgaggacag ctaagcgtca accagctcga
     1201 cggccccccg ggaacggtca acctcgccgc ttacgtccat ctgtcggtcg ccggcgaaat
     1261 cagtcgagca cagggctcgg tgccaccggg acaacagatc gcttggccac gttgagacac
     1321 ctcctggcga aagaccgctc gatgtttcac cactcgcctc gtcgggcctc gtgacgcaat
     1381 cgttaacttc ataggactga tgcctcgcct cttcgacttc tcggctaccg acgggtccga
     1441 ccctgacctg ctcgggggca aaggcgcagg cctggcccgc atgacctcgc tgggtctgag
     1501 ggtccctccc gggttcacca tcacgacgga ggtgtgccgg atcgcaatgg ccaccggggc
     1561 gataccggac gagctgtggc ccgaggtcaa agccgcggtc gagcggctcg agcaatccag
     1621 cggtcgaagc tttggctcag gtcctgcgcc cctcctcttg tcggttcgct ccggtgccaa
     1681 ggtctccatg ccagggatga tggacacggt cctgaacctg ggcatcaacg acgaagtggt
     1741 gaaggcgttg atcgagtggt cgggcgactc ccatttcgcc tgggacgcct atcggcgatt
     1801 cgtccagatg tacggcaatg ttgtgctggg cgttcccgag catcgtttcc aggatgtcct
     1861 gaccgaattg agagattcca agggcgtcgc cgacgactcg gagctcaccg cggaggacct
     1921 ggagatggcc acgactcgat tccgcgacat cgtcgagcag gagaggcccg gtgagctgcc
     1981 cgaggacccg ctccaccaac tggagggggc gatcaccgcg gtg
//
LOCUS       DASTXO010000057         2707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1771803, whole genome shotgun sequence.
ACCESSION   DASTXO010000057 DASTXO010000000
VERSION     DASTXO010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1771803"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VFU96_01120"
     CDS             <1..317
                     /locus_tag="VFU96_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111883.1"
                     /translation="GAAMYLAQLESIREALDAGGENSLGELIRARSPETADRIDDTLG
                     RAITELGAIEGPMRDIALESPETLEPIYEDISTLRTLFESDVVSLLDITLGFSDTDGD
                     TG"
     gene            314..1642
                     /locus_tag="VFU96_01125"
     CDS             314..1642
                     /locus_tag="VFU96_01125"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HEU5111884.1"
                     /translation="MTLPAAIRARAATEVSAASGGFFRIGFGIVGMVLVVRFFARGWI
                     QSLLIEPAYHFSYPGFEWVRVWPEPWMQLHFLVIGLAALGITLGYRFRISAGVFALGL
                     GYVELIDRSLYLNHYYWVVLTAAIMVFLPLNAAYSIDAHRGRSRTPGYYPTWVVWLLR
                     FQVAMVYVFAGLAKLNSDWLLRAEPLSTWLPARSEMWLVGSVLTLPATAYLLSWAGAF
                     FDLTIVGWLSWRPTRPYAYIALVVFHGFTWLMFPSIGLFPLLMSLSALVFFEPAWPER
                     FVFPARVASVGSAPKSEGRHHSAWAGVAAVYVLVMVALPLRHHLTPGDVKWTGEGYLG
                     SWQVMLSEKSASAVFVVTDPASGSSWTVPPPDYLTERQRMVRATDPVMIRQTARLIAD
                     DLGGGVEVAADVRLSFNGRVSQQFTDPEVVISEAPLTEAVEGFILAQPSG"
     gene            1662..2576
                     /locus_tag="VFU96_01130"
     CDS             1662..2576
                     /locus_tag="VFU96_01130"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEU5111885.1"
                     /translation="MADASASSDAEETTPGRIERLKDRYATVKTHSLERLETERERRA
                     SVRVAYDFYRRDQAFAGSLIAGGLSVKVFLWFLPFSLSFVVLLGALADWLGRPPADLA
                     EDTGLINALAGMVAAAVSASSRARLYLAVLGIALLLWAGLGVVRALFLTSRLAWRVSP
                     SKPVRKVVASLSVVGFVLAMVGIQRLTDRLQGGPWIVDVLVYVMAIVAIAALHVLLFN
                     ALPRTEGTPWTAVIPGALLMTAAWLIIRIATVVYFTRRLNSASELYGGLGMAGVFLAW
                     LYIICRALVASISLNATIWQRDQTALPD"
BASE COUNT          398 a          899 c          858 g          552 t
ORIGIN      
        1 atggagcggc catgtatctg gcccaattgg agagcattcg cgaagcgctg gatgccggcg
       61 gcgagaactc gctcggtgag ctgatccgag ccaggtcccc agagaccgcc gatcgaatcg
      121 atgacaccct cggccgcgcg atcacggaac tgggcgccat cgagggcccg atgcgcgata
      181 tcgcgctcga gagccccgag accctcgagc ccatatacga ggacatctcc accctgcgca
      241 cactgttcga gtccgacgtc gtgtcgctcc tggacataac actcggcttc tccgacaccg
      301 acggtgacac cggatgacgc ttcccgcggc gatcagggcg cgagcggcga ccgaggtgag
      361 cgctgcttcg ggtggcttct tccgcatcgg gttcggcatc gtcgggatgg tcctcgtggt
      421 caggttcttc gcccgagggt ggatccagtc cctgctcatc gagcccgcct atcacttctc
      481 ctatcccggc ttcgaatggg tgcgagtctg gccggagccg tggatgcagc ttcatttcct
      541 ggtgatcggc ctggctgctc tcggcatcac ccttggctat cgcttccgga tttcagccgg
      601 tgtcttcgcg ctgggcctcg gctacgtaga gctcatcgat cggagtctct acctcaacca
      661 ctactactgg gtggtcctca ccgcagcgat catggtgttc ctcccactga acgccgccta
      721 ctcgatcgat gcacatcggg gccggtcacg gaccccgggc tactacccga catgggtggt
      781 ctggctgctc cggttccagg tcgcgatggt ctacgtgttt gccgggctgg ccaaactcaa
      841 cagcgactgg ctgctgcggg ccgagcctct ctcgacctgg ctgccggccc gatcagagat
      901 gtggctggtc gggtccgtgt tgacccttcc ggcgaccgcc taccttctct cctgggcggg
      961 cgcgttcttc gatctcacca tcgtcggctg gctgtcgtgg cggccgaccc ggccctacgc
     1021 ctacatcgcg ctggtcgtct tccacggctt tacctggctg atgttcccct ccatagggct
     1081 gttccccctc ctgatgtccc tctccgccct cgtctttttc gagccggctt ggccggagcg
     1141 ctttgtcttc ccagctcgcg ttgcgagcgt cgggagtgcg ccgaagtcag aggggaggca
     1201 ccattcggcc tgggccgggg tcgccgccgt gtacgtgctg gtcatggtgg ccctgccact
     1261 tcgccaccat ctcaccccgg gtgatgtgaa gtggactggt gaggggtatc tcgggtcctg
     1321 gcaggtgatg ctcagcgaga aatcggcgtc ggcggtcttc gtggtcacgg accctgcgtc
     1381 cgggagcagc tggaccgtgc cgcctcccga ctatctcacg gagagacagc gaatggtcag
     1441 ggccaccgac cccgtgatga tccgtcagac ggcacggctc atcgccgacg atcttggagg
     1501 aggagtcgag gtcgccgcgg acgtgcggct ctccttcaac gggcgagtga gccagcagtt
     1561 caccgacccc gaagtagtga tatccgaggc gccgctcacc gaggcggtcg aaggcttcat
     1621 cctcgcccag ccgtcgggct gacaccgggt atcttcggcg gatggccgat gcctccgcat
     1681 ccagcgacgc cgaggagacc acgccagggc gcatcgagcg gctcaaggac cgatacgcca
     1741 ccgtcaagac acacagtctg gagcgtctcg aaacggagcg cgagcggcgc gccagcgtgc
     1801 gggtcgccta cgacttctat cggcgggacc aggcattcgc cggcagtctc atcgccggag
     1861 ggctgtcggt gaaggtcttc ctctggttcc ttcccttctc gctgtcgttc gtggtgttgc
     1921 ttggtgcgct ggccgactgg ctcggtcgcc cgcctgcaga tcttgcggag gacacgggtc
     1981 tgatcaacgc cctggccggg atggtggcag ccgccgtgtc cgcctccagc cgggcccggc
     2041 tctacctggc tgtcctcggc atcgccctcc tcctctgggc cggtctgggc gttgtgcgag
     2101 cactcttcct gaccagtcgg ctcgcatgga gggtgagtcc ctcgaagccg gtccgcaagg
     2161 tggtggcctc gttgtccgtt gtcggattcg ttctggccat ggtcgggatc cagcggctca
     2221 cagaccggct gcagggcggg ccttggatcg tggacgtcct ggtgtacgtg atggcgatcg
     2281 tcgccatcgc cgctctccac gtcctgctct tcaacgcgct gccccggact gaggggactc
     2341 cttggacagc tgtgatcccc ggagcgttgc tcatgactgc cgcctggctc atcatccgaa
     2401 tcgccaccgt tgtctatttc acgagaagac tgaactccgc cagcgagctc tacggcggtc
     2461 tcgggatggc cggagtgttc ctcgcttggc tctacatcat ctgtcgcgct ctcgtcgcct
     2521 cgatctccct gaatgccacg atttggcagc gagaccaaac agctctaccc gattaggcag
     2581 gcgtcgcgat cgaggatttg tggcaggcac ccatccccac tgtgggtgcc tgcacactga
     2641 cgggatcagg cggagatcgc ctgcccgagg acctcctgac cgaagtagat gaggaaggcg
     2701 atcgcca
//
LOCUS       DASTXO010000058         3238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1734878, whole genome shotgun sequence.
ACCESSION   DASTXO010000058 DASTXO010000000
VERSION     DASTXO010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1734878"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VFU96_01135"
     CDS             complement(<1..304)
                     /locus_tag="VFU96_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111886.1"
                     /translation="MSVIRAAKIGLLLLVVACGLDSEAVALAESACSTLEEVQDEPFD
                     PTQLAAYNDWLDANQSGMNSVAVGGGSADYEAALREECGPVVETFAVAFEDQTGTSF"
     gene            468..935
                     /locus_tag="VFU96_01140"
     CDS             468..935
                     /locus_tag="VFU96_01140"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5111887.1"
                     /translation="MVTEKREVDSFTQFVVANEARLRHALIATCGGESGRELAQEVLA
                     YGWEHWERLQSMENPVGYLYVMGRNLSRRRRRVGPVVFSEVPFDRTPYVEPALPKALA
                     ALSTRQRTSVVLVHCFQWTLSEVAEVMGISKTAVQNHLERGMASLRDKIGDVP"
     gene            932..2950
                     /locus_tag="VFU96_01145"
     CDS             932..2950
                     /locus_tag="VFU96_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111888.1"
                     /translation="MISLEQQLADYGQLQDELFGPISIDEIASPIVPSGAVGVGNRPP
                     QTRLGRGIGWAVAAFVIVVLAVGGMYFAFRGGDGQVVDQTTVPTSTPVFESGSVAGTW
                     VTTDADGSTPTMTVQVTEDGIVEIIVLDDVASVCSGAPSTMTGTGGLEDETVLVIPAP
                     TLTCDDGTEPQALEGGSLDDILQDLTFTHDPENDTLSDNIGSSWRREGTEDPSPPPTA
                     ATAMWPQSNLEEVVEAQELADAGHPGYTWQLDATLAANGEPWGAEIFARFIEEELGWE
                     DSSRFGGYASGEGVYDEVLFIRCAPGRSNPLSPFYEDVPPEIRRCAPTIDEFSYETVT
                     VRVTQPGRRGPDGIWVVEQWEMNRPADQGSLWGLLYPDFAFSQVEQVVPPSDVEVRDL
                     LEAFLQARVDGEGAEQYLLREPEGSIFRDHEVPILYATTTGAPYERYEIEKALGPVWP
                     TGWTEYKVGLFAEGGTVVEQYFYVVRQEDGQLGLVYGYKYDGLPTTENGQSVALHYSL
                     LDGEVTFAAAPPWGPGDTSEPSDTLVRLNGSRDDHVVIATDPLPALTECDVPALADAE
                     ALAQRIMADSSAEATGTVPVSIAGLDGLQMDVDVDMSAVLADENSCRWTWSPDQAVRW
                     RMRLYLLDHPGGSAPVLAIAVIAAPETDFEHVLEETTPIVESLEIHSR"
BASE COUNT          586 a          987 c         1088 g          577 t
ORIGIN      
        1 agaacgaggt tccggtctgg tcctcgaacg caacggcgaa tgtctccacc acaggtccac
       61 actcctcgcg gagagcagct tcatagtcag ccgaaccacc gccgactgcc accgagttca
      121 tcccggactg gttggcgtcc aaccagtcgt tgtacgcagc gagctgggtc gggtcgaacg
      181 gctcgtcttg cacctcttcc agggtgctgc aggcgctctc ggccagagct actgcctccg
      241 aatcaagccc gcaggccacc acgagcagca gaagcccgat cttcgccgct cggatcacac
      301 tcatcgccta ctcctctgtt tgttttctca caccgaggac cctaggtcgt gggtgagacg
      361 cctccaacgg gccaactatt cgggcgaatc ttgaggaggc ttccctcgtc ttgtggtacg
      421 ggcggcccgt atcgtgcctt attgggtgtg agagtcgagg gacagtcatg gtgaccgaaa
      481 agcgcgaggt cgattcgttc acccagtttg tcgtcgccaa cgaagcaaga cttcgccacg
      541 cgttgatagc gacatgtggt ggggagtcgg gccgggaact ggcgcaggaa gtcctggcat
      601 atgggtggga gcattgggaa cgtcttcaga gcatggagaa cccggtcggt tacttgtatg
      661 tgatgggccg gaatctgagt cgtcgccgcc ggcgtgtcgg gccagtggtg ttcagcgagg
      721 ttcctttcga ccggactccc tacgtcgagc ctgcccttcc caaggcttta gcggctcttt
      781 cgacccgaca gcggacctct gttgtgttgg tgcactgttt ccagtggacg ctgagcgagg
      841 tcgctgaggt gatggggatc tccaagacgg ccgtccagaa ccacctggag aggggaatgg
      901 catctctcag agacaagatt ggagatgttc catgatcagc ttggagcaac agctcgccga
      961 ctacgggcaa ctgcaggatg agctgtttgg accgatcagc atcgatgaga ttgccagccc
     1021 aattgtgccg tcgggcgccg tcggggtcgg taatcgacca ccgcagacga ggttgggacg
     1081 ggggatcggt tgggcggtgg cggcgttcgt catcgtcgtt ctcgccgtag gcgggatgta
     1141 cttcgccttc cgtggaggtg atggccaggt ggtcgatcaa accaccgtgc ccacctcgac
     1201 cccggttttc gagtccggtt ccgtcgcggg gacctgggtg accaccgacg ccgacggaag
     1261 caccccgacc atgaccgtcc aggtcacgga ggatggcatc gtcgagatca ttgtgctgga
     1321 cgacgtcgcc tccgtttgtt cgggtgcccc ctcgacgatg acgggcaccg gagggctcga
     1381 ggatgagacc gtgctggtga tcccggcgcc gaccctgacc tgcgatgacg gcaccgagcc
     1441 ccaggccctg gagggtgggt ccctggacga catactgcaa gacctgacct tcacccatga
     1501 cccggagaac gacaccctca gcgacaacat cggatcgtcg tggcgccggg aggggaccga
     1561 ggacccgagc ccgccaccga cagcggcgac ggccatgtgg ccccagtcga acttggagga
     1621 agtggtggag gcacaagagc tcgccgacgc cggccacccc ggctacacgt ggcagctcga
     1681 cgcgacgctg gccgccaacg gggagccatg gggggcggag atcttcgccc gtttcatcga
     1741 ggaggagctc ggctgggagg acagctctcg cttcgggggg tacgcatccg gagaaggtgt
     1801 ctacgacgaa gtcctgttca tccgttgcgc ccctggccga agcaaccctc tgagcccatt
     1861 ttacgaggat gtaccgccgg agatccgccg gtgtgcgccg acgatcgatg agttcagcta
     1921 cgagacggtg acggtcaggg tgacccagcc tgggcgtcgg ggaccggatg ggatctgggt
     1981 ggtggaacaa tgggagatga acaggcctgc cgatcagggc tccctctggg gtctcctcta
     2041 ccccgacttt gcctttagcc aggtcgagca ggtcgtcccg ccatcagacg ttgaagtcag
     2101 agacctcctg gaggcgttcc tccaggcccg ggtggatgga gagggggccg agcagtatct
     2161 gctccgcgag ccggaagggt cgattttccg ggaccatgag gtgccaatcc tctacgccac
     2221 gaccaccggc gccccctatg agcgatacga gatcgagaag gccctgggcc cggtgtggcc
     2281 gaccgggtgg acggagtaca aggttggtct tttcgccgag ggtgggaccg tggtcgagca
     2341 gtatttctac gttgtccgcc aggaggacgg gcagctcggt ctggtgtacg gctacaagta
     2401 tgacggcctc cccaccaccg agaacgggca gtccgtcgcc ctgcactaca gcctgctcga
     2461 tggcgaggtg acattcgccg cggccccgcc gtgggggccc ggcgacacct ccgagccttc
     2521 cgacaccctc gtgaggctca acgggagcag agacgatcac gtcgtgatag cgaccgatcc
     2581 cttaccggcc ctgacagagt gcgacgttcc ggctctcgca gatgccgagg cgctggccca
     2641 gaggatcatg gccgattcca gtgccgaggc caccgggacc gtgccggtga gcatcgcggg
     2701 tctcgacggg ctgcagatgg acgtggacgt cgacatgagc gcggtgttgg ccgatgagaa
     2761 ctcctgtcgg tggacgtggt caccggacca ggccgttcgg tggcggatgc gtctctacct
     2821 gctcgaccac cccggcgggt cggccccggt cctggcgatc gcggtcatcg ctgccccgga
     2881 gaccgacttc gagcacgtgc tggaggagac gacacccatc gtggagtctc tcgagatcca
     2941 ttcccgctga tgtgattgag cggctagtcg agaaagaggg agacatgatg agaaggttga
     3001 gaccggcagg agtcgcagcg gctctgctcg tgttgacggc gtgcgcgagc gggggagctg
     3061 caacaacgac ggaacccaca acaaccgccg caccgaccac aaccgccgca cctgccacca
     3121 gcgcgcccac catgtccgat ctggaggtgg tcgaggccgg tgtggctgcg ttctactccg
     3181 gggaggcgga acgggccgcc gagttgttcg aactcgcgga ccgcaccgac gatcagat
//
LOCUS       DASTXO010000059         2295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_924708, whole genome shotgun sequence.
ACCESSION   DASTXO010000059 DASTXO010000000
VERSION     DASTXO010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924708"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VFU96_01150"
     CDS             <1..558
                     /locus_tag="VFU96_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111889.1"
                     /translation="ASAVSVVVRYRGASGVERLQLKWLMAAGLVSALAYGVLFFCEGF
                     SIQLVWASIPIAIGLSMHRHRLYDIDRLISRTVVYAVVVGVLALVFTVVVLVLGNLLP
                     GDDALPVAASTLAVAAIFNPLRRRVQGRVDRFFNRSHYNADLVAARFAGGLRDETDAD
                     RIVQGWVGVVSETMQPTAVGVWTRE"
     gene            748..1617
                     /gene="hisG"
                     /locus_tag="VFU96_01155"
     CDS             748..1617
                     /gene="hisG"
                     /locus_tag="VFU96_01155"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEU5111890.1"
                     /translation="MSTEPTIHLALPKGRMETGVFNLLAAAGIDLHVGQRGYRPMLSE
                     PGFEVKLLKPQNIVEMLAVGTRDIGFAGADWVAEQQANLVELLDTGLDPVRIVAAAPE
                     SLLDEGGGLPARRLVVASEYERLTRSWITARNLDAAFVRSYGATEVFPPEDADVIVDN
                     SATGETLAANGLAVVDELMTSSTRFYASQGAMADPDKKSAIDGLVLSLRSVLDARKRV
                     MLELNVSLADLDAVIDALPAMREPTVATLHGGAGMAVKAAVPRRDLPRLIPRLRQLGA
                     TDIAVSKLEQIVP"
     gene            1614..>2295
                     /locus_tag="VFU96_01160"
     CDS             1614..>2295
                     /locus_tag="VFU96_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5111891.1"
                     /translation="MSSPATYLPPGFAVPIDLDLSKNEGRSRAKELLDAIEDPRRLVG
                     RYPDTSPLRAKLAALHGISEDRVLVTAGGDDALSRCFLARVAEGGRVVGTYPTFEMIP
                     RYTEQRGAELTEVPWWTGPFPVDEVLAAIGEDADAVFIVSPNNPTGGVVSELDLLKVA
                     GEARLLVLDAAYVEFADSDPTHALLDLGNVVVTRTLSKAYGLAGLRVGYLLGSAELVA
                     AIGAYGNPS"
BASE COUNT          372 a          769 c          747 g          407 t
ORIGIN      
        1 gcttcggcgg tctcggttgt ggtccgctat cggggcgcct ccggggtcga gcggctccag
       61 ctcaagtggt tgatggctgc cgggctcgtg tccgcgctcg cctacggcgt cctcttcttc
      121 tgtgagggtt tcagcattca gctggtctgg gcatcgatcc cgatagccat tgggctctcc
      181 atgcaccgtc accgcctata tgacatcgac cggctcatca gccgcacggt cgtctacgcc
      241 gtggtcgtcg gggtactcgc cctcgtcttc accgtggtag tactcgtcct gggaaatctt
      301 ctgccagggg acgatgcgct gcccgtggct gcctcgacat tggcggtggc cgcgatcttc
      361 aatcctctcc ggcgccgggt ccagggccgg gtggaccgat tcttcaatcg ttcgcactac
      421 aacgccgatc ttgtcgctgc ccggttcgcc ggcggccttc gtgacgagac cgacgccgat
      481 cggatcgtgc agggttgggt cggggtggtg tccgagacga tgcagccgac tgctgtcgga
      541 gtgtggacga gagagtgaat aatgcctatg ctcgataacg aaatgaaccg gcttgtcgcc
      601 cacgcccatc atcaccatca ccccgtatgg gtcggatgac gatgggctga ggccgagaag
      661 ccaccaacgc aaacgccgac ccaagcggtc ggcgtttctc gtttccgagg gcgagatccc
      721 ggccgcacgg cgggaaggaa ccccaacatg agcaccgaac caacgatcca cctggccctc
      781 cccaaggggc ggatggagac cggtgtattc aacctgctcg ccgcagccgg catcgacctc
      841 catgtcggtc agcgtggtta tcgcccgatg ctgtccgagc ctggtttcga ggtgaagctc
      901 ctcaagccgc aaaacatcgt cgagatgctc gcggtgggga cccgggacat cggtttcgcc
      961 ggcgccgact gggtagccga acaacaggca aatctggtcg agctgctcga taccgggctc
     1021 gacccggtcc ggatcgtggc cgcggccccg gagagcctgc tcgacgaggg tggcggtctc
     1081 ccggcgagac ggctggtcgt cgccagcgaa tacgagcggc tcacccggtc ctggatcacc
     1141 gctcgcaacc tcgatgccgc tttcgtccgc tcgtatgggg caacggaggt cttcccaccg
     1201 gaagacgccg acgtcatcgt cgacaacagc gccacgggcg agactctcgc ggccaacggg
     1261 ctggccgtcg tcgacgagct gatgacgtcg tccacgcgct tctacgccag ccagggagcg
     1321 atggccgacc cggacaagaa gagcgcaatc gatgggctgg tgttgagcct gcgctcggtc
     1381 ctcgatgccc gcaagagggt catgctcgag ctgaacgtgt cgctggccga tctcgatgca
     1441 gtcatcgacg cgctgcccgc catgcgggag cccaccgtgg caacgctcca cggcggcgcc
     1501 ggcatggcgg tgaaggcggc ggtgccccgc cgggacctgc cccggctcat cccacgtctt
     1561 cgccagctcg gcgcaaccga catcgccgtc tcgaaactgg agcaaatcgt cccatgagct
     1621 cgcccgccac ctatctcccg ccaggcttcg cggtgccgat cgatctcgac ttgtcgaaga
     1681 acgaaggacg aagccgagcc aaggagctcc tcgatgccat cgaggacccg cggcgcctgg
     1741 tgggccgcta cccggacacc tcacccctcc gggccaagct cgccgccctg cacggcatct
     1801 ccgaggatcg ggtcctggtg acggccggcg gggacgatgc gctgtcccga tgcttcctcg
     1861 cccgggtggc tgaaggcggc cgggtggtgg ggacctaccc caccttcgag atgatccctc
     1921 gctacaccga gcagcgtggc gcggagctca cggaagtgcc gtggtggacc ggtccattcc
     1981 cggttgacga ggtcctggca gcgatcggcg aggacgccga cgccgtgttc atcgtgtccc
     2041 cgaacaaccc gactggtggt gtggtcagcg agctcgacct cctcaaggtg gcaggagaag
     2101 cccgattgct cgtcctcgac gccgcatacg tggagttcgc cgactccgac ccgacgcatg
     2161 cgttgctcga tctgggcaat gtcgtggtga cacgaacgtt gtcgaaggcc tacgggctcg
     2221 ccggcctcag ggtggggtat ctgctgggat ccgcggaact cgtcgcggcc atcggcgcct
     2281 atggcaaccc ctctc
//
LOCUS       DASTXO010000060         2043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1514028, whole genome shotgun sequence.
ACCESSION   DASTXO010000060 DASTXO010000000
VERSION     DASTXO010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514028"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2043
                     /locus_tag="VFU96_01165"
     CDS             <1..>2043
                     /locus_tag="VFU96_01165"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEU5111892.1"
                     /translation="DKARDVLGDGLGHVTRVSGASDLGAEAAARLANQIQRFLLDETR
                     LGIPAIVHEEICSGLMSRFAVVYPQAIGVAATWDPDLARDMADAVRVQMRAMGAHHGL
                     SPVLDVARDPRWGRTEETFGEDPYLVARMGVAFVEGLQGDDLTEGVVATAKHFVGYSA
                     SEGGLNWAPPHLPARELREVYLHPFEAVVDAGAWSVMNAYHELDGIPCGADRDLLTVL
                     LRERWGFEGTVVSDYFAVRQLASYHHVAPDAIGAAATALHAGIDVELPGTDCYGEPLI
                     EGVRNGGIDLDLVDTAVRRVLRSKFDLGLFESPFVDPPMTHADPGPERDLARRIATKS
                     IVLLANDGVLPITPGSVSIAVIGPNAHDPRNLFGDYTYPAHIEALVDMQSSDNVFGVP
                     FSGEVSLEGSMKQARSVFDALRDRLGDNVGYAPGCAVDSQSREGFEEAVALARQAGLA
                     VMVMGDKAGLTSECTSGEGRDRLSLDLPGVQEDLVRAIIETGTPVVLILVTGRPCGSQ
                     WAHEHSSAVIQAWLPGEEGAEAIADVLTGVVSPGGKLPISHPRSAGQIPVYYGHKVSG
                     GRSQWKGDYVDGAATPLYPFGHGLAYTTFRVSDLAVTPDVATWNGAVTVRALVTNTGD
                     MDGDEVVQLYVRDPVASVTRPVLELKGFARVGLPARGSARVSFEVPVGQLGFYNRD"
BASE COUNT          328 a          668 c          696 g          351 t
ORIGIN      
        1 acgacaaggc ccgtgacgtg ctcggtgacg gtctcggcca cgtgacccgg gtctctggcg
       61 ccagcgacct cggggcggag gctgcggctc gcctggcaaa tcagatccag cgtttcctcc
      121 tcgacgagac ccgactcggc atcccggcca tcgtccacga ggagatctgc tccgggctga
      181 tgtcgcgctt tgccgtcgtc tatccgcagg ccattggggt tgccgccacc tgggatcccg
      241 atctggcacg ggacatggcc gacgcggtcc gtgtgcagat gcgggcgatg ggcgcccatc
      301 acggtctgtc tcctgtgctc gacgtcgcga gggacccgcg ctggggccgc accgaggaga
      361 ccttcggaga ggacccgtat ctcgttgccc gcatgggagt cgccttcgtc gaagggctgc
      421 aaggcgacga tctgaccgag ggtgtggtgg cgaccgccaa gcatttcgtg ggctacagcg
      481 cttccgaggg agggttgaac tgggccccgc cccatctccc cgcccgggag ctgcgggagg
      541 tctacctgca tccattcgaa gcggtcgttg acgccggggc ctggtcggtg atgaacgcct
      601 accacgagct cgacgggatt ccttgcggcg cggaccgcga cctgctgaca gtgctcctcc
      661 gtgagcgatg gggattcgaa gggaccgtgg tgtccgacta cttcgcggtc agacagctgg
      721 ccagctacca ccacgtcgcc ccggatgcga tcggcgccgc cgcaaccgct cttcacgccg
      781 ggatcgacgt cgagctcccc ggcaccgact gttacgggga gccgctcatc gaaggcgtcc
      841 gcaatggggg catcgatctc gacctcgtcg acacggcggt caggagagtg ctccggtcca
      901 aattcgacct tggtctcttc gaatcgcctt tcgtcgaccc gccgatgacc cacgccgacc
      961 ctggccccga acgcgacctg gcccggcgga tcgccacgaa gagcatcgtg ctgctcgcca
     1021 acgacggcgt gctgcccatc acccccggct cagtgtcgat cgccgtgatc gggcccaatg
     1081 cccacgaccc ccggaatctc ttcggtgatt acacctatcc agcccacatc gaagccctcg
     1141 tcgacatgca gtcgagcgac aatgtgttcg gggtgccgtt ctccggagag gtgagcctcg
     1201 aaggatcgat gaagcaagca cgctctgtgt tcgatgccct tcgggaccgt ttgggggaca
     1261 acgtcgggta cgccccagga tgtgcggtcg atagtcagtc gcgcgaaggc ttcgaggagg
     1321 cggtcgctct ggcgcggcag gcaggcctgg ctgtgatggt catgggggac aaggcgggtc
     1381 tgaccagcga atgcacgtcc ggtgagggcc gcgatcgcct ttcgctcgac cttcccggtg
     1441 tccaggagga cctggtgcgg gcgatcatcg agaccgggac cccggtggtg ctgatactgg
     1501 tgaccggcag accctgcggc agccaatggg cgcacgagca cagctccgcg gtgatccagg
     1561 cctggctgcc gggcgaggag ggagccgagg caatcgccga tgtgctcacc ggtgtggtga
     1621 gcccgggcgg gaaactgccc atctcgcacc cgcggtcggc agggcagatc ccggtctact
     1681 acgggcacaa ggtttctggt ggaaggtccc aatggaaggg cgactacgtc gacggcgcgg
     1741 cgaccccgct ctatccgttc ggtcacggtc tggcctacac gacatttcgc gtctcggacc
     1801 tcgcggtgac accggacgtg gccacatgga acggagcggt cactgtcagg gcgttggtca
     1861 ccaataccgg ggacatggac ggcgacgagg tcgtccagct ctatgtcaga gacccggtgg
     1921 cgagtgtgac ccgaccggtg ctggagctca agggattcgc ccgggtcggg ctccccgcca
     1981 gaggttccgc ccgggtcagc ttcgaggttc ccgtcgggca gctcgggttc tacaaccgcg
     2041 acc
//
LOCUS       DASTXO010000061         2305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_40532, whole genome shotgun sequence.
ACCESSION   DASTXO010000061 DASTXO010000000
VERSION     DASTXO010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40532"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..590
                     /locus_tag="VFU96_01170"
     CDS             225..590
                     /locus_tag="VFU96_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5111893.1"
                     /translation="MGSRILVVEDSAVIRRLIEVCLRAADLEIVTREDGKSGLAAVGS
                     ESPDLVVLDIGLPGMDGWEVLDRIRRDESTRNIPVVVLTAHAEEESRRRANEGGADAF
                     VTKPFQPNDFRSTVLSLLE"
     gene            complement(603..1625)
                     /gene="gap"
                     /locus_tag="VFU96_01175"
     CDS             complement(603..1625)
                     /gene="gap"
                     /locus_tag="VFU96_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU5111894.1"
                     /translation="MANVAINGFGRIGRAVFRIIASRPDSGLKVVAINDLSDDDILAY
                     LLEYDSVMGRFEEDVTVENGVMKVGDHAIKMLMERDPTRLPWKDLDVDIVIESTGVFR
                     DRASLQKHVDSGAKRVILTVPSKDKVDETVVLGVNDDQLDSDDIIVSNASCTTNSLAP
                     LAKVLDDNFGIVKGVMTTVHAYTNDQVLADVPHQDLRRSRAATENIIPTTTGAAAAIG
                     EVVPNLAGKLDGMAMRVPVPDGSTVDLVVELDRDVTVEEVNAAVRAAAEGPMAGILEY
                     VEDPIVSTDIIGNPHSSIFDAGGTQVLGGNLVKVMSWYDNEWGYSNRVVDLAERLADV
                     LEKEPV"
     gene            complement(1628..>2305)
                     /locus_tag="VFU96_01180"
     CDS             complement(1628..>2305)
                     /locus_tag="VFU96_01180"
                     /inference="COORDINATES: protein motif:HMM:NF014818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU5111895.1"
                     /translation="AKKVVLTSSPEIPGEIPLLLRGINDDVLGPDVDQVALGSNTSNA
                     AAPILKTINEGLGIRRAYMTVVKAMSNAGRLADVPTDSYRTSRAAGENIIPAETNSAE
                     IITQALPELEGKLAVVALNVPVVDGSTIDMVVDVERPTEVAEVNELVKTSCESKYQGI
                     IEYVADPIVSSDVRSVSHSGVYDSLATMVTGGNLVKTITWFNNGWGYSNRVVEVTGMV
                     AAYLNGE"
BASE COUNT          421 a          729 c          686 g          469 t
ORIGIN      
        1 gagcctgagg catattggga caccggcgat tccgccatgt cgcactatgc cgcccattgc
       61 acctcggtcc atgaggtgcg gaccttgcgt cagctcgagc aattcatcga ggaattgacc
      121 ctccccggcc cccgtccgct ccagaatgtc taaaccgtga cggacctttt gccgatgttg
      181 tggaacacgc ccgactacgg gcactaggac cagaggtgaa ctacatgggc tcgcggatac
      241 tcgttgtcga ggactcggcc gtcatcaggc ggctcatcga agtctgtctc cgcgccgccg
      301 atctcgagat cgtgacccgc gaggacggga agagtggtct tgccgctgtc gggtcggagt
      361 cacctgatct ggttgtcctg gacatcgggc tccccggcat ggacggatgg gaagtgttgg
      421 accgcatccg tcgggacgag tcaacgagga acattccggt tgtcgtcctc acggcccatg
      481 ccgaagagga atcgcggcgc agggccaatg agggaggggc agacgcattc gtcacgaagc
      541 cgttccagcc caacgacttc cgctcgacgg tcctctcgct gctggaatag ctcgaggccg
      601 gactagaccg gttctttctc cagcacgtcg gccagacgct cggccagatc cacaactcgg
      661 ttcgagtatc cccactcgtt gtcgtaccac gacatcacct tgaccaggtt tcccccaagc
      721 acctgagtgc cacccgcatc gaagatcgac gagtgcggat tcccgatgat gtcggtcgag
      781 acaatgggat cctccacgta ttccaggatc ccggccatag gcccttcggc ggcagctcgg
      841 accgccgcat tcacttcttc caccgtgaca tcacgatcga gctcgaccac gagatcgacc
      901 gtcgagccat cggggaccgg aactctcatc gccataccgt ccagttttcc ggcgaggttg
      961 ggaaccacct ctccgatcgc ggccgcagcg ccggtcgtcg tcgggatgat gttctcggtt
     1021 gcggcccggc tgcggcgcag atcctgatgg ggcacgtcgg ccagcacctg atcgttggtg
     1081 taggcatgga cggtggtcat cacgcctttg actatgccga agttgtcgtc caagaccttg
     1141 gccagtggcg ccagggagtt ggtcgtgcac gaggcattgg agacgatgat gtcgtccgag
     1201 tcgagctgat cgtcgttgac gccgaggacg accgtctcat ccactttgtc cttcgaaggg
     1261 accgtcaaaa tcactcgctt cgccccggaa tcgacgtgct tctgaaggga agcgcggtct
     1321 ctgaaaacgc ccgtggactc gatgacgatg tcgacgtcga ggtccttcca aggcagtcgg
     1381 gtgggatcgc gctccatgag catcttgatc gcgtgatccc cgaccttcat caccccgttc
     1441 tcgacggtca cgtcttcctc gaaccgaccc atcacggagt cgtactccag caaataggcg
     1501 aggatgtcgt catctgagag atcgttgatt gcaacgacct tgagacccga atccggccgt
     1561 gaggcgatga tccgaaagac cgcccggccg attcgcccga acccgttgat tgcgacgttt
     1621 gccatgttca ctctccgttg aggtaggcgg cgaccatgcc ggtgacctcg accactcggt
     1681 tggagtaccc ccaaccgttg ttgaaccagg tgatggtctt caccaggttt ccgcccgtca
     1741 ccatcgtggc caggctgtcg tagacgccgg agtgtgacac cgatctcaca tctgacgaaa
     1801 caatcggatc ggcgacatat tcgatgattc cttgatactt gctctcacat gaggtcttaa
     1861 ccaattcgtt gacctccgcc acctccgtgg ggcgttcgac atccacgacc atgtcgatcg
     1921 tcgagccgtc gacgaccggc acgttgaggg caaccaccgc cagcttgccc tcgagctcgg
     1981 ggagggcctg ggtgatgatc tcggccgagt tggtctcggc agggatgatg ttctctccgg
     2041 ccgcccggct cgtccggtac gaatccgtgg gcacgtcggc cagccgcccg gcattcgaca
     2101 tcgccttgac cactgtcatg taggcacgtc tgatgccgag cccctcattg atggtcttga
     2161 ggatcggcgc tgcggcgttc gacgtgttcg acccgagcgc gacctggtcc acatcggggc
     2221 cgagcacgtc gtcgttgatc ccgcggagca acagcggaat ctcgccgggg atctcggggc
     2281 tcgaggtgag cacgaccttc ttcgc
//
LOCUS       DASTXO010000062         2100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1109013, whole genome shotgun sequence.
ACCESSION   DASTXO010000062 DASTXO010000000
VERSION     DASTXO010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109013"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1840
                     /locus_tag="VFU96_01185"
     CDS             <1..1840
                     /locus_tag="VFU96_01185"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU5111896.1"
                     /translation="SEPVVSEVASVSKPLWLSKDVFGGIPSQPRPDHTPPDHWRLEAV
                     FATARPHHPALSPDGTRIAFVLDIEGTTDLWAITLDDRTLTRITSDRSIVAFWEDSAP
                     VWSPDGTNLAYNSDGSVHVVPLSGGPPRRLLPGSAGAWLDDQRLAVVVERDGFSRLAV
                     VDVDDPWPVPLGPGDGDVGRPQVAPDGRILISYFPKDDFSRSDIVVIDAAGSWATLVG
                     HPDRRAAHHVVNGDRVAYTLEEGEWSGVFVTDLSGADHNQLVGGERDFSSLAWDADDR
                     GLLAVATARGKADLVRIGLDGTIDVLAEGGTWDSPMSVTDGVIAIHEASDSPASVSLV
                     REDGTRSMLYDGAPASVRSAPHSKLERITFKSSDGLEIEGFLYRPADTSRPVPAVVYP
                     HGGPTSYYGDEWDGHAQYFVDKGYAWLAINFRGSTTYGLTFERANHGDWGGGDVEDCL
                     AAAGYLSGLGWVDPNRIAIHGTSYGSYLTFASLVHPENPFACGASKYGGDIDLLTSWA
                     QGDREGREDLERMMGHPANERQAYHAGSPIHQIERISRPLLFAHGERDMRVSAKQAEE
                     LVDALDQVGATYEYFTYPTEGHGLFRREPQLHFYRRLERFLDWYLL"
     gene            1916..>2100
                     /locus_tag="VFU96_01190"
     CDS             1916..>2100
                     /locus_tag="VFU96_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111897.1"
                     /translation="MARRPSSWWIVLSGICAALTAAAVIGAIVVQRESSRLIGEGQIF
                     LADSGVARDIVSTSEDI"
BASE COUNT          359 a          689 c          674 g          378 t
ORIGIN      
        1 ctcggaaccg gtcgtctcgg aggttgcttc ggtgagcaag cccctttggc tcagcaagga
       61 tgtgttcggc ggcatcccga gtcagccacg tcccgatcac acaccacccg accactggcg
      121 gctggaggcc gtgttcgcca cggcccgccc ccaccatccg gcgctcagcc ccgacggcac
      181 ccgtatcgcg ttcgtgctcg acatcgaggg cacgaccgac ctctgggcga tcacgctgga
      241 cgatcgaacg ctcaccagaa tcacgtctga tcgctcaatc gtcgccttct gggaggacag
      301 cgcccccgtt tggtcgcccg acgggaccaa cctggcctac aacagcgatg gcagcgtcca
      361 tgtcgtgcct ctctcaggcg gccccccgcg ccgcctgctt cctggctcgg ccggggcctg
      421 gctcgatgac cagcgcttgg cggtcgtggt cgagcgtgac ggcttttcgc gactggctgt
      481 cgtggatgtc gatgacccgt ggccggttcc actcgggcct ggtgacggtg atgtggggcg
      541 gccacaggtc gccccggatg gccggatact gatctcgtac ttccctaagg acgatttcag
      601 ccgcagtgac atcgtggtca tcgacgcggc gggctcgtgg gccacactgg tggggcatcc
      661 cgatcggcgc gctgcgcatc atgtcgtgaa cggagatcgc gtcgcctaca cgctcgagga
      721 gggggagtgg tcgggtgtct tcgtaaccga tttgagcggc gccgatcaca accagctcgt
      781 cggtggggaa cgggacttct cgtcccttgc ctgggacgcc gatgaccggg ggcttctcgc
      841 cgtcgccacc gcgaggggga aggccgacct ggtccggatt ggtctcgacg ggacaatcga
      901 cgtcctcgcc gagggcggga cctgggactc gccgatgtcg gtcaccgacg gggtcattgc
      961 catccacgag gcaagcgact ccccggcctc ggtcagcctc gttcgggagg atggcacccg
     1021 gtcgatgctc tatgacggtg caccggcctc cgtccgctcg gcgcctcact cgaaactgga
     1081 gaggatcact ttcaaatcat cggacgggtt ggagatcgaa ggctttctgt accggcccgc
     1141 tgacacctcg aggccggttc cagcagtggt atatccacac ggtgggccaa cttcgtacta
     1201 cggggacgag tgggacggtc acgcccaata tttcgtcgac aagggctacg cctggctggc
     1261 gatcaatttc cgtggctcga cgacatacgg cctcactttt gagcgagcca accacggcga
     1321 ctggggtggt ggcgatgtgg aggattgcct ggccgccgcc ggctacctat ccggcctggg
     1381 ctgggtcgat ccgaatcgga tcgccatcca cgggaccagc tacggctcct acctgacttt
     1441 cgcctcgttg gtgcaccccg agaacccgtt tgcttgcggg gcatccaagt acggaggcga
     1501 catcgacctc ctgacctcgt gggcacaggg cgaccgggag ggaagggagg acctggagcg
     1561 aatgatgggc catccggcca acgagcggca ggcgtaccac gccgggtcac ccatccatca
     1621 gatcgaacgc atcagccgtc cgctcctctt tgctcacggt gagagagaca tgagggtcag
     1681 tgccaagcag gcagaagagc tggtcgacgc attggaccag gtcggggcca cctacgaata
     1741 cttcacctat ccgaccgaag gccatggctt gttccgccgc gaacctcagc tccatttcta
     1801 ccgacggctc gagcgattcc tcgactggta tctcctctag ggcgccccgc cgccccatct
     1861 caagaaaact gcccgaaatc ccgattcttt cccggtgaaa cgggtcaaac ccgagatggc
     1921 acggaggccg tcgagctggt ggatcgtgct cagcgggata tgcgccgccc tcaccgccgc
     1981 cgccgtgatc ggggccatcg tcgtgcagcg ggaatcatcg cggctcatcg gtgaaggcca
     2041 gatcttcctc gccgacagcg gagtggcccg ggacatcgtc agcacatcgg aggacatcga
//
LOCUS       DASTXO010000063         4169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1624824, whole genome shotgun sequence.
ACCESSION   DASTXO010000063 DASTXO010000000
VERSION     DASTXO010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624824"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VFU96_01195"
     CDS             complement(<1..538)
                     /locus_tag="VFU96_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013222372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5111898.1"
                     /translation="MSVLGQAAPLIDWDWIARNVGQIADRTWQHLSLTAVSVGIGLII
                     SVGLAIVSLRWRWAYTPVTWVTGLLYTIPSLALFALLVPVVGLNATNAVIALTSYTLL
                     IIIRNLVAGIDGVPNAVLEAADGMGYTRRARFWKMEVPLALPVIIAGLRIATVTTVGL
                     VTVTAVLGLGGYGYFILRG"
     gene            complement(535..1638)
                     /locus_tag="VFU96_01200"
     CDS             complement(535..1638)
                     /locus_tag="VFU96_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5111899.1"
                     /translation="MAGEAMIRLDNVRKVFPGTTRAAVTDLSLDVRRGECITLIGPSG
                     CGKTTTLKMINRIIEPTSGRIEIDGRDAMAIPAHELRRSIGYVIQQIGLFPHRTIAQN
                     IGTVPALLGWDQKRIDERVTELVEVVGLEAEMTSRYPSELSGGQQQRAGVARALAADP
                     PVLLMDEPFGAVDPIVRKRLQSEFHDLQARLGKTVVFVTHDVDEAILLGDRMVVLNIG
                     GIIEQYDTPEKVLAEPASDFVERFLSGERGLKRLALLPISKAHLEPGPFVEVSVTPDD
                     ARRVMTLHGVDWLGLLDGGRLLGWVDGKLVGRTPLEELEPRPFLVTLGSDSSLRSALD
                     AVVTSHARVAVVVDDGIYRGMLDLEAIAEGITE"
     gene            complement(1681..2592)
                     /locus_tag="VFU96_01205"
     CDS             complement(1681..2592)
                     /locus_tag="VFU96_01205"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5111900.1"
                     /translation="MTSRISFWSFFWLVVGLVLVFHIAGGWYFSSELIQDGFEPAPEP
                     ITPVQGPFELEAVSYDSPLGEMDAWYLPADGPTWVIHVHGLGTSPAEAEPLFEPLQGA
                     GYPQLSITYRNDEGQPADPSGYYQYGATEWEDVAGAVDYAVANGAQNVVLSGFSTGGA
                     HVMSFLSRQPQEIVIGVLMDAPNVDCGRTVDYVASQRDLPVLPLPVPATLSASAKFIT
                     SLRIGVNWKLLDYIADADETIRQPMLIHHGTADLRVPLATSLDLQATNPDLIQLITVE
                     GAGHIESYDVDRQGYVDSVHAFLAGLS"
     gene            complement(2614..3483)
                     /locus_tag="VFU96_01210"
     CDS             complement(2614..3483)
                     /locus_tag="VFU96_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111901.1"
                     /translation="MVWTRGNGCSELAALLSEDVPDRITGDLVEQCINHRADLLVSRR
                     FPGSFDLVSVAVPLDFVPQNVRRVVSAVAGGPHSFLAADTASRLGRGLGVESEMLSAI
                     RPGDDPREAEDLLDHIGLSVPEIGGRVVEVEGISELVESLEEGSLVVFGAPGGSWLQR
                     SMFGPGARLRSRAPAGAIMVRSAPDRVFRWMGDPVFVSPMRLADDTLRLHEQRVLAVA
                     DEGLLIGLVRRESLEGAGDSPVGSLMEEALSAKVDETIAEAMVLEPTFGRDPIPVTDH
                     DEHLVGGLSLPVA"
     gene            3654..>4169
                     /locus_tag="VFU96_01215"
     CDS             3654..>4169
                     /locus_tag="VFU96_01215"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU5111902.1"
                     /translation="MEFDEVIRKRRMVRNYAPDPVPPEALDRIVDAGLSAPSAGYSQG
                     MGFVIVTDAGTRNAISVLAEEQEYVAAGFDPWISTAPAHVVITVSESIYRERYRESDK
                     LGPDGTEIDWPVPYWWVDAGAALMAVLLAAVNEGLVAGFLGVHSMRDLASLLDIPEHH
                     SPIGVVTVGHPR"
BASE COUNT          807 a         1377 c         1294 g          691 t
ORIGIN      
        1 gccctcgcag gatgaaatac ccgtacccac cgagaccgag caccgcggtg accgtcacca
       61 gtccgaccgt ggtcacggtg gcgatgcgga gaccggcgat gatcaccgga agagcaaggg
      121 gcacctccat tttccaaaac ctggcacggc gtgtgtaccc catgccgtcg gctgcctcca
      181 agaccgcatt agggactccg tcgatgcctg ccaccaggtt ccggatgatg atgagcagcg
      241 tgtaggaagt gagggcgatc accgcgttgg tggcattgag gccaacgacc ggcaccaaca
      301 gggcgaacag ggcaagggac gggatcgtgt agagcaggcc ggtgacccac gtgacgggcg
      361 tgtaggccca gcgccagcgg agcgagacga tcgccagacc aaccgagatg atcaagccga
      421 tgccaaccga aaccgcggtc agactgaggt gctgccaggt tcggtctgcg atttgcccga
      481 cgttgcgagc gatccaatcc cagtcgatca acggggcagc ctgaccgagg acgctcactc
      541 tgtgattcct tcggcgatcg cctccagatc gagcatccct cgatagatcc catcgtccac
      601 aaccaccgca acgcgggcat ggctggtcac cacggcgtcc agcgcagatc gaagggacga
      661 gtcgcttccc agcgtgacca ggaatggcct cggctccagt tcctcgagtg gggtgcgacc
      721 aacgagcttg ccatcgaccc atcccaggag tcgacccccg tcgagcagcc cgagccagtc
      781 gaccccatgg agtgtcatca ctcgacgggc gtcatcaggc gtcaccgata cctctacgaa
      841 cgggccgggc tcgagatgcg ccttgctgat cgggagcaga gcgagacgct tcaggccgcg
      901 ttccccggag aggaatctct ctacgaagtc gctcgccggc tcggccagta ccttctccgg
      961 cgtgtcgtat tgctcgatga tgcccccgat gttgagcacg accatgcgat ctccgagcag
     1021 gatcgcctcg tccacgtcgt gcgtgacgaa cacaaccgtc ttgcccagac gagcctgaag
     1081 gtcgtggaac tcggattgga gtcgcttcct cacgatcggg tcgacggcac cgaaaggctc
     1141 gtccatcagc aggaccgggg ggtcagctgc gagggcgcgg gccacacccg cccgctgctg
     1201 ctgcccacct gacagctccg aggggtaccg ggaggtcatc tcggcctcca gccccaccac
     1261 ctcgaccagt tcggtcactc gttcgtcgat tctcttctgg tcccagccga ggagggcggg
     1321 aacggtgccg atgttctggg cgatggtgcg atgggggaac agcccgatct gctgtatcac
     1381 gtagccgatc gaacgacgca actcgtgggc aggaatggcc atcgcatccc tcccgtcgat
     1441 ctcgatccgg ccggaggtgg gctcgatgat ccggttgatc atcttcagcg tggtggtctt
     1501 cccgcatccc gatgggccaa tcagtgtgat gcactcaccg cggcggacgt ccaggctgag
     1561 atcagtaact gcggcgcggg tcgtcccggg gaagaccttc cgcacgttgt cgagacgaat
     1621 catcgcttcg ccggccacct gcacctcctc ggcgccaggg ggacagtagg tgagccgagg
     1681 tcaactcagg ccggcgagaa atgcgtgcac agagtcgaca tagccctgcc ggtcgacgtc
     1741 gtacgactcg atatgaccgg cgccttcgac cgtgatcagc tggatcaggt cggggttcgt
     1801 cgcctggaga tcgagactcg tcgcaagtgg cacccgcaga tccgccgtcc catggtgtat
     1861 gagcatcggt tggcgaatgg tctcgtcggc atcagctatg tagtcgagga gcttccagtt
     1921 caccccgatg cgcaacgagg tgatgaactt ggccgaggcc gacagcgtgg ccgggaccgg
     1981 aaggggcaac accggcagat ctcgctgact cgcgacatag tccacggtcc ggccgcagtc
     2041 gacgttgggc gcgtccatga gcactccgat cacgatctcc tgcggctgcc gggagagaaa
     2101 cgacatcacg tgggcgccac cggtcgagaa cccgctgagc accacgttct gcgcaccatt
     2161 ggccacggcg tagtccactg ctcccgcaac gtcctcccac tcggtggccc cgtattggta
     2221 gtagcccgac ggatccgccg gttgaccttc gtcgttgcga taggtgatcg acaattgcgg
     2281 atagcccgca ccctgcaacg gctcgaacaa aggctcggct tcagccggac tggtccccag
     2341 gccgtggaca tggatgaccc aggttgggcc gtcggcgggc agataccagg cgtccatctc
     2401 gcccaacggt gagtcataac tcaccgcttc caactcgaat gggccctgaa ccggggtgat
     2461 cggctctggg gcgggctcga acccgtcctg gatcagctcg ctcgagaagt accagccgcc
     2521 ggcgatgtgg aacaccagca ccagtccgac cacgagccag aagaaactcc aaaacgaaat
     2581 cctggatgtc actggcggag attctgcacg gaatcaagcc actggcaatg acaaaccgcc
     2641 gaccaggtgc tcgtcatggt cggtgaccgg aatgggatcc cggccaaaag tcggctcgag
     2701 caccatggct tcggcgatgg tttcgtcgac cttcgccgac agagcctcct ccatcaggga
     2761 gccgaccggc gagtctccgg caccctccaa gctctcccga cgaacgagac cgatcagcaa
     2821 gccctcgtcg gcgactgcga gcactctctg ctcatggagg cggagggtgt cgtcggcgag
     2881 acgcatcggg ctgacgaaga ctggatcacc catccatcgg aacacccggt ccggagcgct
     2941 tcgcaccatg atggcaccgg ccggcgccct gctccgcagc cgggctccgg gcccgaacat
     3001 cgaccgctgc agccaagacc ctccaggagc cccgaagaca acgagcgatc cttcctcgag
     3061 actctccacg agctcggata tgccctcgac ttcgacgacc cggcccccta tctctggtac
     3121 cgacaacccg atgtggtcga ggagatcttc ggcttctcgt ggatcgtctc cgggtcgaat
     3181 ggcagacaac atctcggact cgacgccgag cccacgcccg aggcgggagg cggtgtcggc
     3241 agccaggaag gaatgagggc caccggccac agccgacacc acgcgtcgta cgttctgcgg
     3301 cacgaagtcg agaggcacgg cgacactcac caggtcaaac gagccgggga acctgcgcga
     3361 tacgagcaga tcggcccgat ggttgatgca ctgctcgacc aggtcgccgg tgatgcggtc
     3421 ggggacatcc tccgagagga gagccgccag ttcggagcaa ccattacccc gagtccaaac
     3481 caccgtcatc tcgttccccg ccgctggggc cactcgccca ctccctccgt gatcaatcgc
     3541 ggtcctggct tgggcgaccc cgaaccgccg acctcaccat aagacattca acgacccatg
     3601 tcaccgatca gtttcggcct tcggtcatgc ctcgcaagtg agaacattgg gggatggagt
     3661 tcgacgaggt catccgcaaa cgtcgcatgg ttcgcaatta cgcccccgac ccggtaccac
     3721 cggaagctct cgaccggatc gtcgatgccg ggctgagcgc gccgtcggcc gggtacagcc
     3781 agggcatggg attcgtgatc gtgaccgacg ccggcacccg caacgcaatc tccgtgctcg
     3841 ccgaagaaca ggagtacgtg gccgccggat tcgatccctg gatctcgacg gcgccggctc
     3901 atgtcgtgat aaccgtcagc gagtcgatct accgggagcg ctaccgagag tcggacaagc
     3961 tcgggcccga cgggaccgag atcgattggc cggtccccta ttggtgggtg gacgccggag
     4021 cggccctgat ggccgtcctc cttgccgcgg tgaacgaagg gcttgtcgca ggtttcctcg
     4081 gtgtccattc gatgcgcgat ctggcttcct tgctcgacat ccccgagcac cacagcccga
     4141 tcggcgtggt gaccgtcgga catcccagg
//
LOCUS       DASTXO010000064         2483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1293080, whole genome shotgun sequence.
ACCESSION   DASTXO010000064 DASTXO010000000
VERSION     DASTXO010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293080"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1948
                     /locus_tag="VFU96_01220"
     CDS             <1..1948
                     /locus_tag="VFU96_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111903.1"
                     /translation="PNVQDLRDVDFPNTVQDIIIGRFDRLSARQQSVLKVASVIGRDF
                     SVETLVEVYPAALDVTEAIESLQTLVVLDLVHEVTLDRSRYRFRHAITRDIAYGLLLF
                     SQKSQLHRAVALSMEAAHADDLDSVATTLAYHWRNSLSAESESGDVDKALDYLGRAGR
                     ISLRNFAHREAIGFLTDAIDLATTNSDARPSQEVPARTLARLEQDLAEAYLGMGRIDR
                     SADHFERSLELHGYPVGHGKFGVGARLLTASALQARHRLSRSDVAASHGESREDLIEA
                     ATAYERLMKIYYYANDPGALITAAVSGLNLAEKAGTSPVLARIYANMSIVAGIVPLHK
                     LARLYSRRSREVADEVDRPPEHAWVRLATSVYGVGVGDWETAEQDLRDSLDLYKRLGD
                     VRQMEENLGTTGQMLTAQGRFAEVYAIGEQLRESGSGRDDAQGELWGWLFQGTSLLRR
                     GDYRTALSRFRNGESLLDAGVGAINAGWLYGLMAETYWSLGDQERARAAAASATQALY
                     AERPGVVFALDGFTGVADVYLGQWETGRARAASAAKSASELAVKKLRAFSKVFPIGQP
                     AAHRYAGGVKAQAGDLEAARKEWERGIETAADLQMPYEEGRLRLSLGRLSKDRAHLES
                     ARDLFENSGAAPLSDAARSALREI"
     gene            complement(1952..>2483)
                     /locus_tag="VFU96_01225"
     CDS             complement(1952..>2483)
                     /locus_tag="VFU96_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111904.1"
                     /translation="KLSHSMDDKPLTDDLLHCWFLDPDIQREKWLESLPSPGPAGVTG
                     LVPMDEDAEPLFVAYVNTWRLRPQAFTAISNLMLASDYVRTFTSVATMEAANEAARRA
                     VNAIIEHSGHRASLCRLWNVTEPPFAFIFRMIDQRRYERGLPWKEPPFFVKGATKAIT
                     LARPAASGLRRVLGRS"
BASE COUNT          435 a          778 c          828 g          442 t
ORIGIN      
        1 acccaacgtc caggacctgc gtgacgtgga cttcccgaac accgttcagg acatcatcat
       61 cggtcgcttc gatcgtttgt cggcccgcca gcagtcggtc ctgaaggtgg caagcgtgat
      121 cggccgagac ttctcggtcg agaccctggt cgaggtctac ccggccgccc tggacgtgac
      181 cgaagccatc gagagtttgc agactctcgt agttctcgat ctcgtgcatg aagtgaccct
      241 cgaccgatcc aggtatcgct tccggcatgc catcacccgc gacatcgcgt acgggttgct
      301 tctcttctcg cagaagagtc agctccatcg cgcagtagcc ctctcgatgg aggcggctca
      361 cgccgacgat ctcgactcgg tggccaccac gctcgcctac cactggcgta acagcctgag
      421 tgcggagtcg gagagtggtg acgtcgacaa ggcactggac tacttgggtc gggccggccg
      481 gatctctttg cgaaatttcg cccatcgcga ggcaatcggc tttctcaccg acgccatcga
      541 cctggcgaca accaacagcg acgcccggcc gagccaggag gtgccggctc ggacgttggc
      601 ccggctggaa caagacctgg ccgaggccta cctcggcatg ggccggatcg atcgcagtgc
      661 ggaccacttc gaacggtcac tcgagctcca tggctacccg gtcggtcatg gcaaattcgg
      721 cgtcggggcc cggcttctca ccgcgtctgc tctgcaggca cgccatcggt tgtctcgatc
      781 tgacgtcgcc gcctcgcatg gcgagtcgag ggaggacctg atcgaggcgg ccaccgccta
      841 tgagcggttg atgaagatct actactacgc caacgatccc ggggccttga tcaccgccgc
      901 cgtttcgggg ctgaacctcg ccgagaaggc aggcacctcg ccggtgctcg ctcgcatcta
      961 cgcaaatatg agcatcgtcg ccggcatcgt gcccctccat aagttggcgc ggctctactc
     1021 ccgaagaagc agggaggtcg ccgacgaagt cgaccgcccg ccagagcacg cctgggttcg
     1081 tttggccacc agtgtctacg gggttggtgt cggtgattgg gagacggccg agcaggacct
     1141 gagagactct ctcgacctct acaagcgtct cggtgacgtg cgtcagatgg aggagaacct
     1201 gggcacgacg gggcagatgt tgaccgccca gggccgcttc gccgaggtct acgcgatcgg
     1261 cgagcagctc agggagtcgg gctcgggccg ggatgacgcg cagggtgagc tgtggggttg
     1321 gctcttccag gggaccagcc tgctccgccg gggcgactat cgaaccgcac tgtcgaggtt
     1381 tcgaaatggt gagtctcttc tcgatgccgg agtgggggcg atcaatgcgg gctggctcta
     1441 cggtttgatg gcggagactt attggagcct cggtgaccag gaacgcgccc gggcggcagc
     1501 agcctcggcg actcaggcgc tctacgccga gcggccaggt gtcgtgttcg ccctcgacgg
     1561 attcactggc gtcgccgacg tctatctcgg tcaatgggag accgggcgcg cccgcgctgc
     1621 ctcggccgcc aagtcggcct ccgaactagc cgtgaagaag ttgcgggcct tcagcaaggt
     1681 attcccgatc ggtcagccgg ccgcccaccg ttatgccgga ggagtcaagg cacaagcggg
     1741 tgacctggag gcggcccgca aggagtggga gcggggaatc gagactgccg ccgacctcca
     1801 gatgccctac gaggaaggca gattgcggct gagcttgggt cgtctgtcca aggacagggc
     1861 gcatctcgaa tcagcccgtg atctgttcga gaacagcggg gccgcgcccc tctcggacgc
     1921 agcccggtcc gcgctcaggg agatctgagc tctaggaccg gccgaggacg cgtcgcagtc
     1981 ccgaggcggc aggcctggcc agggtgattg ccttggtggc gcccttgacg aagaacggag
     2041 gttccttcca gggcaggccg cgctcgtagc gacgttggtc gatcatccgg aagatgaacg
     2101 cgaatggtgg ttcggtgacg ttccaaaggc ggcacagact cgcccgatgc cccgagtgct
     2161 cgatgatggc gttgacggcg cggcgggccg cctcattggc agcctccatc gtcgccaccg
     2221 atgtgaacgt ccgcacgtag tccgaagcca gcatcagatt cgagatcgcc gtgaaggctt
     2281 ggggtcggag ccgccatgtg ttgacatatg caacgaagag cggctccgcg tcctcgtcca
     2341 tcgggaccag accggtgaca ccggccggcc ccggggaggg caatgattcg agccacttct
     2401 cccgctgtat gtccgggtcg aggaaccagc aatgcagcag gtcatcggtc agcggcttgt
     2461 cgtccatgct gtgggagagc ttc
//
LOCUS       DASTXO010000065         2771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_814267, whole genome shotgun sequence.
ACCESSION   DASTXO010000065 DASTXO010000000
VERSION     DASTXO010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814267"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..824
                     /locus_tag="VFU96_01230"
     CDS             66..824
                     /locus_tag="VFU96_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111905.1"
                     /translation="MRTRAHLAAVLAAVFVLTACGDAPFTSLGQRSSGWIGEPTVVTT
                     TTVATTVPVVVDARALKWFNDELGGGSLDDPAALKVAVFARRGGDLFIQASRAEIAAL
                     LPEIKFPTSTPYLSEYVTSQLVFDERGDLADDPVAAFGIWSSEPYTRSRSVAQMIILS
                     VSVDPEVAADVASPGADNSCERFAARSTEVCAVATVDGRPVWSLEAANGTTMVWFDGD
                     YRYELFGRSYVAVEALQRMVVGLVPLSELGTTPG"
     gene            973..2736
                     /locus_tag="VFU96_01235"
     CDS             973..2736
                     /locus_tag="VFU96_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5111906.1"
                     /translation="MIGVLITILIISGLGVVPPILMGEIVDVILAGDTSRLNILGLAL
                     IVVPVISAAVGVAQRWWSSNVGEGIIFDLRCELYSHLQKMSLRFFTATKTGELMSRLN
                     SDVEGAQSAITGTFVSIVSNVVSVVFVLVVMIRLDWRLTLLAVAALPLFVIPARRVGR
                     VLRRITQQLMDHEARMSGILQETFNVSGAMLVRLFGRWDQEAERFGEEAALVRDTSVR
                     RSMVGRGFFAALGLVSAVGTAAVFWVGGLLVIRGDIDVGAIVTFSALLAQLYGPLSAI
                     TNSRVEFATSLVSFERVFEVLDLQTDIPDPVTPEPLILVRGRVEFANVSFRYQGDGPE
                     GLEAVRRWRRTAPDAPVSRASTREWAIRNIDFVSEPGSLTALVGPSGAGKTTISYLLP
                     RLYDVTEGSVLVDGRDVRNIALRDLAAAVGVVTQETYLFHDTIAANLRYAKPDATQGE
                     IEEAARSANIHDMITRLADGYETIVGERGYRLSGGEKQRIAIARVLLEDPRILILDEA
                     TSHLDSRSEALIQEALEKVREGRTSIVIAHRLSTVRAADQILVIDEGEIVERGNHASL
                     VQANGLYASLYQTQFVEPASA"
BASE COUNT          464 a          890 c          886 g          531 t
ORIGIN      
        1 aggctcgttg acaaggagaa gtcggtctcc aagccgagtg ggggcccatg cgactcggag
       61 ggaatttgcg tacccgtgcc catctggcag cggttctggc cgcggtcttc gtattgaccg
      121 cttgtggtga cgcgccattc acctcccttg gccagcgcag ctctgggtgg atcggcgagc
      181 ccacggtggt gaccacgaca accgtggcga cgacggttcc ggtcgtggtg gacgccagag
      241 ccctcaagtg gttcaacgac gagcttggtg gcgggtccct cgacgatccg gcagcgctca
      301 aggtcgcggt gttcgcccgc cgcggtggcg acctgttcat acaagcgagc cgcgccgaga
      361 tcgcagctct cctacccgag atcaagtttc cgacgtctac tccatatcta tcggagtacg
      421 tgacctcaca gttggtgttc gacgagagag gagacctcgc cgacgacccg gtcgccgcct
      481 ttgggatctg gtcatccgag ccatacacca ggtctcgctc ggtggcccag atgatcatcc
      541 tgagtgtctc cgtcgatccc gaggtcgccg ccgatgtcgc atccccgggt gccgacaact
      601 catgtgagcg gttcgccgcg cgaagcaccg aggtgtgcgc cgtggccacg gtcgacggcc
      661 ggcccgtatg gtcgctcgag gcggcgaacg gaacgacgat ggtctggttc gacggcgact
      721 acagatacga gctcttcggg cgctcctatg ttgccgtgga ggccctccag aggatggtcg
      781 tcggcctcgt cccgctatcg gagctaggga ctacccccgg ataaccttca ctggcctagc
      841 tggaggcgac gcatatatca ctccggtggc tggtggagct acctgcagta cgacgaggac
      901 caggaccgcc ccgacgtcga tcgggctctc ctccgccgcg tctggtccta cggtcgaccc
      961 tatgtcggag ggttgatcgg tgtcctgatc acgatcctca tcatctccgg cctgggcgtg
     1021 gtcccaccga tcctcatggg agagatcgtt gatgtgatcc tggctgggga taccagccgg
     1081 ctgaacatcc tcggcctcgc tctgatcgtc gtccccgtga tcagtgccgc agtcggggtg
     1141 gcccagcggt ggtggtcgtc gaatgtcggg gaggggatca tcttcgatct caggtgcgag
     1201 ctctacagcc acctgcagaa gatgtcgctg cggttcttca ccgcaacaaa gaccggggag
     1261 ctgatgagcc ggctcaacag cgatgtcgag ggcgcccagt ccgcgatcac cggcacattc
     1321 gtctccatcg tctccaatgt ggtctcggtg gttttcgtcc tcgtggtgat gatccgtctc
     1381 gactggcggc tcactctcct cgccgtggct gccctgcccc ttttcgtcat accggcccgc
     1441 cgcgttggaa gggtgctgcg acggatcaca cagcagctca tggaccatga ggcccggatg
     1501 tcgggcatct tgcaggagac cttcaacgtg agcggggcaa tgctggtccg actgttcggc
     1561 cgctgggatc aagaggcgga gcgcttcggc gaagaggccg ccctggtccg cgatacgtcg
     1621 gtgaggaggt ccatggtggg caggggcttc ttcgccgcgc tgggcctggt ctctgcggtg
     1681 ggaactgcgg ccgtcttctg ggttgggggc ctgctcgtca tccggggtga catcgacgtc
     1741 ggcgccatcg tcaccttctc agctctgctc gcccagctct acggaccgct ctcggcgatc
     1801 accaactcgc gggtcgaatt cgccacatcg cttgtctctt tcgagcgagt cttcgaagta
     1861 ctcgacctcc aaaccgacat cccggatccg gtgacaccgg agccgctcat cctggttcgc
     1921 gggcgggtgg agttcgccaa tgtctctttc cgctaccagg gcgacgggcc cgaggggctc
     1981 gaggccgtgc gacgatggag acggaccgca cccgatgccc cagtgtcccg tgccagcacc
     2041 agagaatggg ccatccgcaa catcgacttc gtgtccgaac cgggttcgct gaccgccttg
     2101 gtcgggccat ctggcgccgg caagaccacg atctcatatc tccttcccag gctctacgac
     2161 gtcaccgagg gatcggtgct cgtggatggg cgcgatgtgc gcaacattgc gttgcgcgat
     2221 ctggcagcag cggtcggagt cgtgacccag gagacgtacc tgttccacga cacgatcgcc
     2281 gccaacctga ggtacgcaaa acccgatgcc actcagggag agatcgagga ggcggcacgg
     2341 tcggcgaaca tccatgacat gatcaccagg ctggctgacg gctacgagac gatcgtgggg
     2401 gagaggggat accgtctctc gggcggggag aagcaacgca ttgcgatcgc ccgcgtcctg
     2461 ctcgaagacc cccggatcct catcctcgac gaggcaacct cccatctcga ctccagatcc
     2521 gaggccctga tccaggaagc tctggagaag gtgagggagg gccggacctc gatcgtcatc
     2581 gcccaccgtc tctccaccgt gcgcgccgcc gatcagatcc tggtcatcga cgagggagaa
     2641 atcgtcgagc gtggcaacca cgcgtcactg gtacaggcca acgggctcta cgcgtccctg
     2701 tatcagaccc agttcgtcga acccgcgtcc gcctgatcgg tcacgcctcc cgggtgaggt
     2761 tctcgatcag c
//
LOCUS       DASTXO010000066         2386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417 k141_4000,
            whole genome shotgun sequence.
ACCESSION   DASTXO010000066 DASTXO010000000
VERSION     DASTXO010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4000"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..158)
                     /locus_tag="VFU96_01240"
     CDS             complement(<1..158)
                     /locus_tag="VFU96_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005987363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5111907.1"
                     /translation="MVAAEHQNDPGSIRAWITYDWANSAYATTILAAVLPAYFAAEVV
                     GEDGFALFG"
     gene            188..1387
                     /locus_tag="VFU96_01245"
     CDS             188..1387
                     /locus_tag="VFU96_01245"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEU5111908.1"
                     /translation="MRRRLIPLLCVLAACTAEPESEPTTSTIPAVTTSTSADTTLPET
                     TSTTAEAEVSTTVPTTSTLALDATVLGYENVADLNFPVQLTARAGEANSYVITKDGRI
                     WLYDGSAIAAEPVLDILDRVRNDGEQGLLSMALHPTDPSRFYLHYSDTSGDTVVSEFT
                     LSGPDRADPASERVLLQVDQPAPNHNGGMIQFTPDGALILGLGAGGGAGDSVENGQNP
                     DTLLGGLVAIDVDGGEPAASKYAMGLRNPWRFWIDGEMIYIADVGQGAYEEVSVTPLE
                     PGLNYGWPVLEGLHCFNTSDCDGTGMVAPILEVEHGDGGTCSITGGPVYRGTSLPQLR
                     GHYFYSDYCGGYLRSLLFADGAATGLRDWSEQVGVPGQVTGFGVDGAGEMYVTTTNQL
                     LKVVAGG"
     gene            1411..1833
                     /locus_tag="VFU96_01250"
     CDS             1411..1833
                     /locus_tag="VFU96_01250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU5111909.1"
                     /translation="MPTLFTRIIEGEIPGTFVWRDDQCVAFLSINPLRRGHTLVVPKL
                     EIDHWIDCPPELSNHLFTVAGRIGAALQTVFQPTRIGLMIAGLEVPHMHIHVVPMDGV
                     HDLDFSNAATSVEREDLEDAADAIRTELAVMGLSGASS"
     gene            1834..>2386
                     /locus_tag="VFU96_01255"
     CDS             1834..>2386
                     /locus_tag="VFU96_01255"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU5111910.1"
                     /translation="MEVVLGGISSLLYGVADFLGGEGAKRAPAAAIVLWAGVISFPMI
                     LVVALIVGGDAGPADLLLGAAAGIAGALGLVSLFAGLSQGNAAAVAPTAAAVAAVLPV
                     IVAVVDGERPSGLAWIGVALAVPAIVLSSWVADPGETPKGRFGYGLAAGLGFGGFTAI
                     IGFTVPESNLLPLITSRAASMLAV"
BASE COUNT          374 a          776 c          794 g          442 t
ORIGIN      
        1 ccgaagaggg cgaacccgtc ctcgccgacg acctcggcag cgaaatacgc cgggaggacg
       61 gccgccagga tcgtggtggc gtatgccgag ttcgcccagt cgtaggtgat ccaggcccgg
      121 atgctgcccg gatcgttctg gtgctcggcg gccactattg gcgcaacata tacgtagcgt
      181 gtcgaagatg cgccgtcgtc tgatccccct cctgtgcgtg ctggccgcat gcaccgccga
      241 gcccgaatcg gagcccacaa cctccaccat cccggccgtc acgacgtcga cgagtgcgga
      301 caccaccctc cccgagacga cgtctacgac ggccgaagct gaagtctcga ccaccgtccc
      361 gaccacctcg acactcgccc tcgacgccac ggtcctcggt tatgagaacg tcgccgatct
      421 caacttccct gttcagctga ccgcccgcgc cggagaggcg aattcctatg tgatcaccaa
      481 ggatgggcgc atctggctct acgacgggag cgccatcgca gccgagcctg tgctggacat
      541 cttggacaga gtgcgcaacg acggggagca gggattgctc tcgatggcgc tccacccgac
      601 agacccctca cggttctatc tccactattc ggacacctcg ggcgacaccg tcgtctctga
      661 gttcacgttg tccggtccgg accgagccga cccggccagc gagcgggtct tgctccaggt
      721 cgatcagcct gcgccgaatc acaacggtgg gatgatccag ttcacgcccg atggcgccct
      781 gatcctcggc ctcggcgccg gggggggagc gggagattcg gttgagaacg gccagaatcc
      841 cgacacgctg ctgggcgggc tcgtggccat cgacgtcgac ggcggcgagc ccgccgcaag
      901 caaatacgcc atgggcctgc gcaacccatg gcggttctgg atcgacggcg agatgatcta
      961 tatcgccgat gttggccagg gcgcctacga ggaggtctcg gtaacgccgc tggagccggg
     1021 tctgaactac ggatggccgg tgctggaagg gctgcactgc ttcaacacgt ctgattgtga
     1081 cgggacgggg atggtggcgc cgatcctcga ggtcgagcac ggggatggcg ggacctgttc
     1141 gataaccggt ggccccgtct atcggggcac ctcacttccc cagctgcgcg gccactactt
     1201 ctactccgat tactgcgggg ggtatctccg cagcctcctc ttcgcagatg gtgccgcaac
     1261 ggggctgcga gactggtctg aacaggtcgg cgtccccggt caggtgaccg gattcggggt
     1321 cgacggtgcg ggcgagatgt atgtcaccac gaccaaccag ttgctgaagg tggttgcggg
     1381 cggctgaacc ggcgcggata ggctcggcgg atgccgacac tgttcacgcg catcatcgag
     1441 ggagagatac ccggcacgtt cgtgtggcgt gatgatcagt gtgtcgcctt cctctctatc
     1501 aatcccttgc gacgggggca caccctggtg gtgccgaagc tggagatcga ccattggatc
     1561 gattgcccac ccgagctgtc gaaccacctg ttcactgtcg ccggccgcat cggcgcggct
     1621 ctccagaccg ttttccagcc gactcggatc gggctgatga tcgccggttt ggaggttccc
     1681 cacatgcaca tccatgtggt ccctatggac ggcgtgcacg acctcgattt ctccaatgcg
     1741 gccacgtcgg tggagcgtga ggacctcgag gatgccgcgg atgcgattcg aaccgagctc
     1801 gcggtcatgg gcctgtccgg ggccagcagc taggtggagg tagtcctcgg aggcatcagc
     1861 tccctcctct atggagtcgc cgacttcctg ggtggagagg gggccaagcg cgccccggcg
     1921 gcggccatcg tcctctgggc cggggtgatc agcttcccca tgatcctcgt cgtcgccctc
     1981 atcgtcggtg gcgacgccgg ccctgcggac ctcctgctcg gagcagcagc cgggattgcg
     2041 ggcgctcttg gtctcgtgtc cttgtttgcc gggctcagcc agggaaatgc cgctgccgtc
     2101 gcacctaccg ctgccgccgt cgccgccgtg cttccggtga tcgtggctgt cgtcgacggc
     2161 gagcgaccga gtgggctcgc ctggatcggc gtggcgttgg cggttccggc gatcgttctc
     2221 agctcgtggg tggcggaccc gggcgagacg ccaaagggca gattcggata cgggctggcc
     2281 gccgggctcg ggttcggcgg tttcaccgcg atcatcgggt tcaccgttcc tgagtcgaat
     2341 ctgctcccgc tcatcacgtc tcgggcggcg tcgatgctgg cggtgt
//
LOCUS       DASTXO010000067         4531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1661730, whole genome shotgun sequence.
ACCESSION   DASTXO010000067 DASTXO010000000
VERSION     DASTXO010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1661730"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VFU96_01260"
     CDS             <1..770
                     /locus_tag="VFU96_01260"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5111911.1"
                     /translation="HYHVPPEVGAAFARIQNDALIELSEREPTRFHVFATLPLQDIAA
                     SLAEIGRVAAFSRVRGVQIGSNIDGTDVDDPSLEPVWADLERRDFPVWFHSDQRSIAG
                     ADRMSSYYLQNLIGIPLESTIAAAKLIFGGVLERHPGLRIGFTHGGGFSPYQIGRWEH
                     GWTVRPEPKVNIADTGPRQFFGTMYFDSLTHDPLSLEMLGRRMGWDHVLLGSDYPFDM
                     ASTDPVGGVEMVEMSDDDRARVLHQNAERFLRPLEDS"
     gene            770..1702
                     /locus_tag="VFU96_01265"
     CDS             770..1702
                     /locus_tag="VFU96_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU5111912.1"
                     /translation="MRTAHIGGLVVSVIGLGCNNFGRALDKAGSAAVVEAALDAGITH
                     FDTASNYGEGQSESFLGAALSGRRDDVVIATKVGVAIPGWEGSGGAAPDYVAKVLERC
                     LTQLGTDYVDIYMIHFPDPNTPMEDTLAVMSGLVDEGKVRQIGCSNFDPAQLNEALAV
                     SAEHGWPPFVCDQVQYSMIHREPETDGLADLCVESRVALLPYYPLANGLLTGKTRRGG
                     EPQGRLKMDRYQDFLTEENFDLVEGIESFASERGVTMVQVALGWLLAQDAVPAVAPGA
                     TSPAQVVANAKAAEWSPNDDDLTALRSLLGPYAA"
     gene            complement(1690..2118)
                     /locus_tag="VFU96_01270"
     CDS             complement(1690..2118)
                     /locus_tag="VFU96_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111913.1"
                     /translation="MIGRRLLLAVFSLLGMFVAACGDPCEQRLGEYPPDHIVVTGVEA
                     VDVPHIGWACPGFHSDTIEPPPSVAPDPEGNVLVEITMEPGSTIDVRFGNQPVVVEPA
                     PASGSNSWVLQVPAPSEPLVIRLCSEEQACALYWANTYAA"
     gene            2190..3023
                     /locus_tag="VFU96_01275"
     CDS             2190..3023
                     /locus_tag="VFU96_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015495615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEU5111914.1"
                     /translation="MPTSRSFIQVDVFSPIPTQGNGLAVVIDAEGLDDTAMQGFAAWT
                     NLAETTFLLPPKDTTADYRVRIFTPHQELPFAGHPTLGSCVAWLHSGGVPKEPGVIRQ
                     ECGIGIVEIDSTAERLAFVAPPTSVSPLPPDRFDAIAGGLGIPLEAVLQTATLDNGPV
                     WQVLDLGSAEEVLEVDGSKARWSPLELVGLLGAYVPGSECDFEVRMLASNGVEDPITG
                     SLNAALAYWLRDQDRLGDGLVISQGTALGRHGRVYIRPADDRILIGGDTHILIEGAVT
                     L"
     gene            3026..3799
                     /locus_tag="VFU96_01280"
     CDS             3026..3799
                     /locus_tag="VFU96_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HEU5111915.1"
                     /translation="MSGDHFELDEEFEVDELNTEIWFPYYLPHWSSREESAATWFIRD
                     GELHLLIPADQPLWCPGLHEEPLRVSGIQTGSFAGPVGSTTGQQPFEDGLTVREQQPT
                     FWGYTPRYGRIAGRMRGALTPRSMFGFWLAGIEDRPERSGEILVAEVFGDAIEDGSAA
                     VGMGIRSFRDPGLTGDFSTPRLEIDVSEPHTYAVVWNPAFVEIVVDDVPVRRIGQSPG
                     YPMQLMIGVFDVPARDPSGIWADHVPELVVSSVKGAPLS"
     gene            complement(3748..>4531)
                     /locus_tag="VFU96_01285"
     CDS             complement(3748..>4531)
                     /locus_tag="VFU96_01285"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEU5111916.1"
                     /translation="SDAMSVLRQADVPLTEEPRAAIRGLAGLARLANWPAPEAVEPID
                     CAFEEWGMPLVDGDIARTADEAVAVAKSLGYPVVVKVVSGGVAHKTEVGGVRVDLHDA
                     SAVGHAIDEVTAAALAAGLPVEGVRVERYRPGLEMLIGGLVDSAFGPLISIGLGGVLT
                     ELLDDIVYAPAPVDEIQAASMIERLRGRALLDGFRGTPQADIDELARIVSLVSRGMTG
                     SGLREVEINPLIWDGESWVAVDWLLIQERGAPLTEETTSSGT"
BASE COUNT          805 a         1498 c         1440 g          788 t
ORIGIN      
        1 tccactatca cgtgccacct gaggttgggg ccgcgtttgc caggatccag aacgatgctc
       61 tgatcgaact cagcgagcga gagcccaccc ggttccacgt cttcgccaca cttcccctgc
      121 aggacatcgc cgcctcgctc gccgagatcg ggcgggtggc ggcgttctcc cgggttcgcg
      181 gagtacagat cggttccaac atcgacggca ccgatgtcga cgacccgtcg ctggagccgg
      241 tatgggcaga cctggagcgc cgggattttc cggtctggtt ccattcggat cagcgctcca
      301 tcgccggcgc agaccggatg agctcgtact acttgcagaa tctgatcggc atccccctcg
      361 agtccaccat cgccgctgcg aagctgatct tcggtggggt tctcgaaagg catccgggcc
      421 tccgtatcgg attcacccac ggtggcgggt tctcccccta tcagatcggg cgctgggagc
      481 acgggtggac ggtgcggccc gagcccaagg tcaacatcgc cgacacgggt cccaggcaat
      541 tcttcgggac gatgtacttc gacagcctca cccatgaccc gctttcgctg gagatgctgg
      601 gacggaggat ggggtgggac cacgtcctcc tcggcagcga ctatcccttc gatatggcct
      661 cgacggaccc ggtgggcggc gtggagatgg tggagatgag cgacgacgac cgggcccggg
      721 ttctccacca gaacgccgag cgcttcctgc gaccgttgga ggattcctga tgcgtacggc
      781 acacatcgga ggtctcgtgg tctctgtcat cggcctcggc tgcaacaact tcgggcgggc
      841 cctggacaag gccgggtcgg cggccgtggt cgaggcggca ttggatgccg gcatcaccca
      901 cttcgacacc gccagcaact acggggaggg ccagtcggag tccttcctcg gcgccgccct
      961 gagtggacgc agggacgatg tcgtcatcgc caccaaggtc ggcgtagcca ttcccggctg
     1021 ggagggaagc ggtggagctg cccctgacta tgtcgccaag gtgctggaac ggtgtctgac
     1081 ccagctcggc accgattacg tcgacatcta catgatccat ttccccgacc cgaatacccc
     1141 catggaggac acgctggcag tgatgagcgg actagtggac gagggaaaag tgcggcagat
     1201 cggatgttcc aacttcgatc ctgcccagct caacgaggcg ttggcggtct ccgccgagca
     1261 cggatggccc ccctttgttt gcgaccaggt ccaatacagc atgatccatc gggagccgga
     1321 gaccgacggg ctggccgatc tgtgtgtcga atcgcgggtg gccctactcc cttactaccc
     1381 gctggcaaat ggcttgctca ccggcaagac gcggcgaggc ggcgagcccc agggacggtt
     1441 gaagatggac cgctatcagg atttcctcac cgaggagaac ttcgatctgg tcgagggaat
     1501 cgagagtttc gcctcggagc gcggggtcac catggtccag gtggcattgg gctggctgct
     1561 ggcccaggat gccgtgcccg cagttgcgcc gggcgccacc agcccggcac aggtagtggc
     1621 caacgcgaag gcggcggagt ggagcccgaa tgacgatgac ctgacagctc tcaggagcct
     1681 gctcggcccc tacgccgcat aagtgttggc ccaatagagg gcgcaggcct gctcctccga
     1741 gcagagtcgg atgaccaatg gctccgatgg agccggcacc tgcagcaccc aggagttgga
     1801 gccactcgct ggggccggct ccaccactac cggctgattg ccgaaccgaa cgtcgatcgt
     1861 gctccccggc tccatggtga tctcgacgag gacattccct tccgggtcgg gtgccaccga
     1921 agggggtggc tcgattgtgt ccgaatggaa accgggacag gcccatccga tgtgggggac
     1981 gtcgacggct tcgacgccgg tgaccacgat gtgatccggc gggtattcgc cgagacgctg
     2041 ctcgcacgga tcaccgcacg cagcaacgaa catcccgagc aatgagaaaa cggcgagcag
     2101 gagacgacgg ccgatcaagg acggccaatg taggaaacct cgtccaccga ccagaatctg
     2161 gtggaccgca acggagctag gttcgctcga tgccgacatc acgaagtttc atccaggtcg
     2221 acgtgttcag tcccattccc acccagggaa acgggctcgc agtagtaata gacgcagaag
     2281 ggctcgacga caccgcgatg cagggcttcg ccgcctggac caatctcgcc gagaccacat
     2341 tcttgcttcc accgaaggac acgacagccg attaccgcgt ccgtatcttc acgccacacc
     2401 aggagctgcc cttcgcgggg cacccgacgt tgggcagctg cgtcgcttgg ctccactccg
     2461 ggggcgtccc caaggagccc ggggtgattc gccaggaatg tgggatcggc atcgtcgaaa
     2521 tagacagtac ggctgagcgt ctcgccttcg tggcgccccc cacgtcggtg tctcctctcc
     2581 ctcccgaccg tttcgatgcc atcgccggag ggctgggcat tccactcgag gcagttctcc
     2641 agacggcgac actcgacaat gggcctgtgt ggcaggtgct cgatctcggg tcggccgagg
     2701 aggtgctcga ggtcgacggg tccaaggccc gctggtcgcc gctcgagttg gtgggactgc
     2761 tcggggctta cgttcctggg tccgaatgcg atttcgaggt aaggatgctc gcctccaacg
     2821 gcgtcgagga cccgatcacc gggtcgttga acgcagccct cgcctactgg ctgcgagatc
     2881 aagaccggct tggtgatggg ctggtgatct cccagggcac cgcattggga cgacatggcc
     2941 gcgtctacat tcggccggcg gatgaccgca tcttgatcgg tggcgacacg cacatcctca
     3001 tcgagggcgc ggtcacgctg tagcgatgtc cggcgatcac ttcgagctcg acgaggaatt
     3061 cgaagtcgac gagctgaaca ccgagatctg gttcccgtat tacctgccgc attggagctc
     3121 gcgagaggaa tcggcggcca cctggttcat ccgcgacggc gagctgcacc tactgatccc
     3181 tgccgatcag ccgctctggt gccccggcct gcacgaggag ccactccggg tctcgggaat
     3241 ccagacgggc agcttcgccg ggccggtcgg ctcgacgact ggccaacagc cgttcgaaga
     3301 tgggctgacg gtgagggagc agcaaccgac gttttggggc tacacacccc gctacggacg
     3361 aatcgcgggg cggatgcgcg gcgcattgac accgcgctcg atgttcggtt tctggctagc
     3421 gggtatcgag gatcggcccg agcgatccgg cgagatcctg gttgccgagg tctttgggga
     3481 cgcgatcgag gacggctcgg ccgcggtcgg gatgggcatc cgtagtttcc gcgatccggg
     3541 tctcaccggc gacttctcca ccccgcgtct cgagatcgat gtgtccgagc cccataccta
     3601 cgctgtcgtc tggaatcctg ccttcgtcga gatcgtcgtc gacgacgtgc cggtgcgccg
     3661 aatcggccaa tcgcccggct atcccatgca gctcatgatc ggcgtgttcg acgtcccggc
     3721 ccgggaccct tcggggatct gggccgatca cgtgcccgag ctggtcgtct cctcggtcaa
     3781 gggggctccc ctctcctgaa tcaggagcca atcgaccgcc acccagctct cgccatccca
     3841 gatcaacgga ttgatctcta cctctcgcag tccggatccc gtcatcccac ggctgaccag
     3901 gctcacgatc cgggccagct cgtcgatgtc ggcctgcggg gtcccccgaa acccgtccag
     3961 cagcgcccgg ccccggagac gctcgatcat cgacgccgcc tggatctcgt cgaccggggc
     4021 cggggcgtag acgatgtcgt cgagcaattc ggtgagcaca ccacccaggc cgatcgagat
     4081 gagcgggccg aacgcggagt cgaccaggcc accgatgagc atctccagcc ccggccgata
     4141 ccgctcaacc ctcactcctt cgacgggcag acccgcggcc aacgccgccg cagtcacctc
     4201 gtcgatggcg tgaccgacag ctgatgcatc gtgcagatcc acccgaaccc ctcccacctc
     4261 ggtcttgtgg gccacgccac ccgacaccac cttgaccacg accgggtacc cgagactctt
     4321 tgcgacggcg acggcctcgt cggcggtccg ggcaatgtca ccatcgacaa ggggcattcc
     4381 ccattcctcg aaagcgcaat cgatcggctc cactgcctcc ggagcaggcc aattcgccaa
     4441 ccgggccaag cctgccagcc ctcggattgc cgccctcggc tcctcggtga ggggaacatc
     4501 cgcctgcctc aagacggaca tggcgtccga t
//
LOCUS       DASTXO010000068         2311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1809093, whole genome shotgun sequence.
ACCESSION   DASTXO010000068 DASTXO010000000
VERSION     DASTXO010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1809093"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..608)
                     /locus_tag="VFU96_01290"
     CDS             complement(3..608)
                     /locus_tag="VFU96_01290"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEU5111917.1"
                     /translation="MIVEVIAVGTELLIGQIANTNAAFIGARLAEEGLDAHYQVTVGD
                     NLDRLVMAIDDGLSRADAIILTGGIGPTQDDLTREAMAHVAGLPMTRDAEHAAWIEAR
                     VASQSGSVNPTVLRMADLPEGAQGLRNANGVALGVALEHEGKWMFAVPGVPVEMRAMI
                     DCEVMPRLRRLAGEATVLRSRLLRTWGIGESSIAEALDDLF"
     gene            complement(605..1786)
                     /locus_tag="VFU96_01295"
     CDS             complement(605..1786)
                     /locus_tag="VFU96_01295"
                     /inference="COORDINATES: protein motif:HMM:NF005566.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HEU5111918.1"
                     /translation="MGWPRVLVAGGSIGGLTAGVLLRDLGCEVDVFERSQAALEDRGA
                     GIVVLPITERYFTERGGEDDRVSLELTFWTYMDREGTVLSADPDHYRFSGWSTIYRSL
                     LEAFGPDRYHLGKEMIAFDNQRNGVTLHLADGSSVEGDLLVCADGLLSTARSILMPET
                     APRYAGYVAWRGTTPEAALSDRARADLADSMIYQVLDHGHLLVYAIPDHHGRTTPPHR
                     VINFVWYRNYPEGGPFEDLMLDRHQVHRAGTMPPGMVREKHLTEMRSIAADVLAPTLL
                     EVVMGCDEPLIQAVFDLESPQMAFGNVCLIGDAAIGLRPHVAAGQAKACADGWALRDA
                     LIASDGDVTSALAAWEPGQLALGHHALARTREMGVASQFDGTMVPGDPTWKFGLWEPG
                     N"
     gene            complement(1797..2174)
                     /locus_tag="VFU96_01300"
     CDS             complement(1797..2174)
                     /locus_tag="VFU96_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111919.1"
                     /translation="MEARPEIVDEGVYDPADFKDELAAAPDNFDVGTTVWFENDRIRV
                     WEILLQPGERGAFHSHVTNYFWTVVEGSRGLQRFADGTFVVRDYVVGETKYLEHTPES
                     ALIHDLENVGESPLRFVTVELLG"
BASE COUNT          421 a          759 c          726 g          405 t
ORIGIN      
        1 gactagaaca aatcatcgag ggcctcggcg atactcgact cgccgatgcc ccaggtgcga
       61 agaagccggc tgcgcagtac ggtcgcttct ccggccaatc tccgaaggcg cggcatgacc
      121 tcacagtcga tcatggccct catctccacc gggacaccgg ggacggcgaa catccatttt
      181 ccttcatgtt ccagggccac tccgagggca acgccgttgg cgttgcgcag accctgggca
      241 ccttctggca ggtccgccat ccgcaacacg gttgggttca cggagccgga ttgggacgcg
      301 acccgggctt cgatccaagc cgcgtgctcg gcgtcccgtg tcatcggcaa cccggcgaca
      361 tgggccatcg cctcccgggt caggtcgtcc tgagtcggcc cgatcccacc ggtgaggatg
      421 atggcatcgg cgcgggacag accgtcatcg atcgccatga ccaaccggtc gaggttgtca
      481 ccgacggtga cctggtagtg ggcgtcgaga ccctcctcgg cgaggcgggc cccgatgaag
      541 gcggcattgg tgttggcgat ctgcccgatc aagagctcgg tcccgactgc gatcacttcg
      601 acgatcaatt tcccggctcc cacaaaccga atttccaggt gggatcgccg gggaccatgg
      661 tcccgtcgaa ctggctggcc acacccatct cccgggtccg ggcaagggca tgatgaccca
      721 gggccagttg tcccggctcc cacgcggcca aggcggaggt cacgtcgcca tcgctggcga
      781 tcagcgcgtc ccggagcgcc catccatcgg cgcaagcctt ggcttgacct gcggcgacat
      841 gaggacggag cccgatggcg gcgtcaccga tgagacacac attgccgaag gccatctgtg
      901 ggctctccag gtcgaatacc gcctggatca gaggctcatc acaacccatg acgacttcga
      961 gcagagtcgg cgccaggacg tcagcggcaa tcgaccgcat ctcggtcaga tgcttctccc
     1021 ggaccatccc cggtggcatg gtccccgccc gatgcacttg gtgccggtcg agcatcagat
     1081 cctcgaacgg gccgccttcg gggtagttgc ggtaccagac aaagttgatg acccgatggg
     1141 gaggcgtggt tcggccgtga tgatcgggta tggcgtagac gaggagatgg ccgtggtcga
     1201 gcacctggta gatcatcgaa tcggccaaat cggcccgagc ccgatcggac agggccgcct
     1261 ccggagtggt cccccgccag gcgacatagc cggcgtaccg cggcgctgtc tcgggcatga
     1321 ggatcgaacg ggcagtggac aacagaccgt cggcacagac gaggaggtcg ccttccaccg
     1381 acgagccgtc ggcgagatgg agcgtcaccc cgttccgctg attgtcgaag gcgatcatct
     1441 ccttgccgag gtggtagcga tcaggtccga aagcctccaa gagggatcgg tagatagtgc
     1501 tccatccgct gaaccgatag tgatccggat cggcgctgag cacggttcct tcccgatcca
     1561 tgtaggtcca gaaggtgagc tcgaggctga cccgatcgtc ctctcctccc cgctctgtga
     1621 agtagcgctc ggtaatgggg aggacgacga ttccagcgcc gcggtcctcg agagcggcct
     1681 ggcttcgctc gaacacatcg acctcgcatc cgaggtcacg aagcagaact cccgcagtca
     1741 gcccgccgat cgacccaccg gcgacgagga cccgcggcca ccccatagcg gccctgtcag
     1801 cccagaagct cgacggtcac gaagcgaagc gggctctcgc cgacattctc gaggtcgtgg
     1861 atgagcgcac tctccggggt gtgctccagg tacttggtct cgccgaccac gtagtcgcgc
     1921 acgacgaagg tgccatcggc gaaccgctgc agaccccggc tgccttccac caccgtccaa
     1981 aagtagttcg tgacgtggga gtggaaggcg ccccgctcgc cgggctggag gagtatctcc
     2041 cacaccctga tccggtcgtt ctcgaaccag acggtggttc cgacgtcgaa gttgtccggg
     2101 gccgcggcca actcgtcctt gaaatccgca gggtcgtaca ccccctcgtc gacgatctct
     2161 ggtcttgcct ccactaattc acctgatagt ccggtttctc gccggtgaga accttgatga
     2221 cctgtgtggc tgcctttcgc tgcgactcgg caagggcctc ttctgagtag taagccatgt
     2281 gcggggtgac gatcagattc ggcacgcctt c
//
LOCUS       DASTXO010000069         4798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1366972, whole genome shotgun sequence.
ACCESSION   DASTXO010000069 DASTXO010000000
VERSION     DASTXO010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366972"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..1196
                     /locus_tag="VFU96_01305"
     CDS             228..1196
                     /locus_tag="VFU96_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013017746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5111920.1"
                     /translation="MAELQRVIVGQDRVLERVLVALIAGGHCLLEGVPGLGKTLTVAT
                     LARTLGGTFTRIQFTPDLLPSDIVGTRIYRPSTERFDVELGPVFANFVLADEINRAPA
                     KVQSALLEVMAERQISIGGTTYPLPLPFLVLATQNPIESEGVYPLPEAQRDRFLMRIP
                     VDYPTPDQEHEIVNRSRHQISASPVALLSTDDVAGLQAAAAAIYVDRAVQDYAIRLVL
                     ATRNPAEHRVAIDGLLAYGASPRASIGLINAGRALALLRGRDHLLPQDVYDIAYDVLN
                     HRLVLSFDAVADGVTVDDVLVEVLTTVVSPRLSSSQLAGNRTSLAV"
     gene            1304..2167
                     /locus_tag="VFU96_01310"
     CDS             1304..2167
                     /locus_tag="VFU96_01310"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEU5111921.1"
                     /translation="MRRLNGLLAGDHAGLFPGHGTERGESRPYVPGDDPRHIDWAVTA
                     RTHDTHVRDMVADHELDVWLVLDSSSSVGFGTARATKHEVGWSAAGAFALLASNSGNR
                     VGAARSTNHRDRSRPLLIPARGGSAHAGAVLTSLLEPPEDGQPGDLARAIEIVGRAAR
                     RRGMAVIISDFLGSPDWVRPMRAMSHRHEVIAVELVDPREIDLPDVGLIAVVDPETGR
                     RRLIDTSEDRVRERYAALAADHRAEVAGRLKSCGADHLVLRTDRDWVLDFVRFVSGRK
                     ARLMSAGRSGR"
     gene            2164..3147
                     /locus_tag="VFU96_01315"
     CDS             2164..3147
                     /locus_tag="VFU96_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU5111922.1"
                     /translation="MTDFGFLSPGRLAILILPAALAVGYLVLQARRRRYAVRFTTVEM
                     LDQVAPDRPGWRRHLPAVGLIVAAIVATMAFARPVVAGETRESSRLVVLAIDTSISME
                     ADDVTPSRVDAAREAAGAFLDSVPEGVAIGVVGFDGVARNLISPTTRIDAVRRVIDGS
                     INHSGLGEGTAIGEAVFLGIEAIEASGADTDAVAPSDEAIGTIVVLSDGETTMGRSND
                     EAATAARSAGIPVHTIAFGTDAGTIVDPFEGEVPVPVNEEALAKLARQTDGKALSAAS
                     VEQLSQVYEDLGRSVTVEKEQVEIGDWFAGAALGLLCLAGIGSLVWFGRLP"
     gene            complement(3176..4405)
                     /locus_tag="VFU96_01320"
     CDS             complement(3176..4405)
                     /locus_tag="VFU96_01320"
                     /inference="COORDINATES: protein motif:HMM:NF015963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HEU5111923.1"
                     /translation="MTTSSIDDGSWANWVGNQSFSPRQIVRVDSEADVQREVAKAAAD
                     RYGIRTVGTGHSFTPIVETDVLLDTSPLRGLIDVDPGRLTVTAGPKTTIGDFGDPLWE
                     HGLALANQGDIDTQAIAGAIATATHGSGLKQPNLSAVLEGARIVDGSGNVMEISRDVN
                     TDVLPALQTSIGLLGIMTEVTLKVVPAYNLHARVDIMKYDEVMDRFQADIEEYRHFGL
                     FWMPTEASAALYNLHDAGADDCVVKRYREVEVGTSPEGLPDNERIDRCYRIYPMVYDP
                     NFHEVEYFLPIEQTREIMDGMRRLMLDWLPLSVYPLEIRVVAADEAWLSPNYQRDNLV
                     VSISGEPGTDYWPYLRACDALFAEFGGRPHWGKLHFMTADRIAGLFPRYQDFLEMRRR
                     FDPNGTFLNSHLRPLFE"
     gene            4527..>4798
                     /locus_tag="VFU96_01325"
     CDS             4527..>4798
                     /locus_tag="VFU96_01325"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEU5111924.1"
                     /translation="MSEKEHVAKVSTVVDAPKEDVWDALTDPDSISRYMFGTKVNTDW
                     QEGSPITWEGEWEGKSYQDKGTIREVRPPQILEYSHFSPLTGLPDVP"
BASE COUNT          812 a         1483 c         1620 g          883 t
ORIGIN      
        1 ggccctcaag gaccaggagg tctcgaggat ctccccggcc tcgaggagat gctcgaagag
       61 atgttcggtg cggccgacat cccaccggaa ctacaacagc tgctcgaaca actgctcggg
      121 atcccggctc cggccgctcc cggagagggg gagacgtcat gaccacgagt cagttcacga
      181 ccagagtcga attgtcggcc gttcatgggg tgcaactccg tgaggtgatg gctgagctgc
      241 agcgggtcat cgtcggccag gaccgggtcc tcgagcgtgt cctcgtcgca ctcatcgctg
      301 gtggtcactg ccttctcgag ggcgtccccg gcctgggcaa gacgctcacg gtcgccacgc
      361 tggcgaggac actcggtggc accttcaccc gaattcagtt cactcccgat ctgctgccgt
      421 cagacatcgt cggcacccgc atctaccggc cttcgacgga gcggttcgac gtcgaactgg
      481 gcccggtatt cgccaacttc gtactggcag acgagatcaa ccgtgcaccg gccaaggtcc
      541 agtcggcgct cctcgaggtc atggccgagc gccagatctc gatcgggggc accacctatc
      601 cgctgcccct tccgttcttg gtgctggcca cccagaaccc gatcgagtcg gagggcgtat
      661 acccactgcc cgaggcgcag cgggaccgtt tcctcatgag gatcccagtg gactacccga
      721 caccggatca ggagcacgag atcgtcaacc gttcccggca ccagatctcg gcgtcaccgg
      781 tcgctcttct gtccaccgat gacgtggccg ggcttcaggc cgcggcggcc gccatctacg
      841 tcgacagggc ggtgcaggac tacgccatac ggctggtcct cgccacccgt aacccggcag
      901 aacaccgagt ggcgatcgat ggtctcttgg cctacggtgc cagcccccgt gccagcatcg
      961 ggctgatcaa tgccggacgt gccctcgccc tcctgcgagg tcgggatcat ctcctcccac
     1021 aggatgtcta cgacatcgcc tacgacgtgc tcaaccatcg tctggtgctg tcgttcgatg
     1081 ctgtggccga tggggtcacg gtggacgacg tcctggtcga ggttctcacg acggtggtgt
     1141 ctccacgcct gtcgtcctcg cagctcgcgg gcaaccggac gtccctggcg gtctgatgcc
     1201 gctcaaacgt tcgggtcctc ccaaggaggt cgagccgatc ccgcgggcat cgcgcctgac
     1261 atcaccgtcg caggtcccaa tcgaacggct gcggctcaca gtcatgcgac ggctcaatgg
     1321 gctccttgcc ggtgaccatg ccgggctctt cccgggtcac gggaccgaga ggggtgaatc
     1381 ccgcccttat gtgcccggcg acgatccacg tcacatcgac tgggcggtga cagcccggac
     1441 acatgacacc catgtccgcg acatggtcgc cgaccacgag ttggatgtgt ggctggtcct
     1501 cgactcgtcg tcgagtgtcg gcttcggcac cgcccgggca accaagcatg aagtgggctg
     1561 gtcggcggcg ggagcatttg ccctgctcgc ctcgaacagc ggcaaccgcg tcggagcggc
     1621 gcgttcgacc aaccatcggg atcgatcacg gcccctcctc attcccgctc gtggcggctc
     1681 ggcccacgcc ggtgcagtcc tgacatcact gctcgagcca ccggaggacg ggcagcccgg
     1741 cgatttggcc cgggccatcg agatcgtggg acgggccgcc cggcggcgag ggatggcagt
     1801 gatcatctcg gatttcctcg gttccccgga ctgggtccgt ccgatgcggg ccatgtccca
     1861 tcgccatgaa gtgatcgcgg tcgaactggt ggatccccgg gagatcgatc tgccagatgt
     1921 cggcttgatt gcagttgtcg atccggagac gggcagaagg cgattgatcg acacttccga
     1981 ggaccgggtt cgcgagcgtt atgccgcgct cgccgccgat caccgcgccg aggtggcggg
     2041 ccggctgaag agctgcggcg ccgaccattt ggtcctgcga acggatcggg attgggtcct
     2101 cgacttcgtt cggtttgttt cgggccgcaa ggcgagattg atgtcggcgg ggaggtcggg
     2161 acgatgacag atttcggatt tctctcaccg gggcggctgg caattctcat cctgcctgct
     2221 gcactcgccg tgggctatct cgtgctccag gcgaggcgtc gccgctacgc cgtgcgcttc
     2281 acgacggttg agatgctcga tcaggtggcc ccggaccgcc cgggatggag gcgtcacctg
     2341 ccggcggttg gtctgatcgt cgcggcaatc gttgcgacca tggcgttcgc ccgcccggtg
     2401 gttgccggag agacgcgtga gtcgtcgcgg ctcgtggtcc tcgccatcga cacgtcgatc
     2461 tcgatggagg ccgatgatgt gacgccatcc cgggtcgacg cggcacgtga ggcggccggt
     2521 gccttcctcg actcggtgcc cgaaggggtc gccatcggag tggtcggctt cgacggtgtg
     2581 gcaagaaacc tgatctcccc gacgacgcgg attgatgcgg tgcgtcgtgt catcgatgga
     2641 tcgatcaacc acagcgggct gggagaaggg actgcgatcg gcgaggcggt attcctcggt
     2701 atcgaggcga tcgaagcctc cggggcggat acagacgccg tggcgccttc cgacgaggcc
     2761 atcggaacga tcgtggtgct ctcggatggt gagacgacga tgggccgtag caacgacgag
     2821 gctgccacgg cggcacggag cgccgggatc ccggtccaca ccatcgcctt cggcacggat
     2881 gccgggacca tcgtggaccc cttcgaaggt gaggtcccgg tcccggtgaa cgaggaagct
     2941 ctggccaaac tggcccgtca gacagacgga aaggcgctga gtgcggcgtc cgtcgagcag
     3001 ctcagccagg tctacgagga tctgggtcgc tcggtgaccg tcgagaagga gcaggtcgag
     3061 atcggcgact ggtttgccgg cgcggcgcta ggtcttctct gtctcgccgg gatcggatcg
     3121 ctggtctggt tcggccggct cccctgagcg acgcggctcg tgctgcatcg aggggtcact
     3181 cgaacagcgg gcggaggtgc gagttgagga aggttccgtt gggatcgaat cgccggcgca
     3241 tctccaagaa gtcctgatag cgagggaaga gacccgcgat acggtccgcg gtcatgaagt
     3301 ggagcttgcc ccagtgtggc cggccgccga actcggcgaa cagggcgtcg caggctcgca
     3361 ggtatggcca gtagtcggtt cctggctccc cggagatcga gacgacgaga ttgtctcgct
     3421 ggtagttagg gctcagccac gcttcgtcgg cggcaacgac gcggatctcc agcgggtaaa
     3481 cgctgagcgg cagccagtcg agcatcagcc gacgcatccc gtccatgatc tcccgggttt
     3541 gctcgatggg caggaagtat tcgacctcat ggaaattggg gtcgtagacc atcgggtaga
     3601 tgcggtagca acgatcgatt cgctcgttgt cggggagccc ttcgggtgag gtgccgactt
     3661 cgacctcccg gtaccgcttg acgacgcagt cgtcggcgcc ggcatcatgg aggttgtaga
     3721 gagctgccga cgcttcggtc ggcatccaga acaggccgaa gtggcggtac tcctcgatat
     3781 ctgcctggaa ccggtccatc acttcgtcgt acttcatgat gtcgacgcgg gcgtgcaggt
     3841 tgtatgcggg aacgaccttc agcgtgactt cggtcatgat gccgagcaga ccgatcgagg
     3901 tctgaagggc cgggagcaca tccgtgttca catcacgtga gatctccatg acgttccccg
     3961 agccgtcgac gatcctggcc ccttcgagta ccgcggagag gttgggctgt ttcagccctg
     4021 aaccgtgagt tgcggtggcg atcgcgccgg cgattgcctg ggtgtcgata tccccctgat
     4081 tggcgagggc cagaccgtgc tcccagagcg ggtcgccgaa atcaccgatg gtggtcttcg
     4141 gtccggccgt caccgtcagg cgacccggat cgacatcgat gagtcctcgg aggggactgg
     4201 tatcgagcag gacgtcggtc tcgacgatgg gagtgaagga gtgcccggtg ccgaccgtgc
     4261 ggatgccgta tcgatcagcg gcagctttgg cgacctcgcg ttgcacgtcg gcctcggaat
     4321 cgaccctcac gatttgtctc ggcgagaagg actggttgcc gacccaattg gcccacgacc
     4381 cgtcgtcgat cgaactggtt gtcacggggc gggaatgtac ccagacactc ttggcccgtg
     4441 accggttcgg gttcgccttc gagcggacga gtgcatggat tacggcgaat tcgggtagga
     4501 ctcgtgtgtc cgacgagagg aagacgatgt cagagaagga acacgtggcg aaggtgtcga
     4561 ccgtcgtcga tgccccgaag gaagacgttt gggatgcctt gaccgacccc gactcgatca
     4621 gtcgatacat gtttgggacg aaggtgaaca ccgattggca ggaggggagc ccgatcacct
     4681 gggaagggga gtgggagggc aagtcgtatc aggacaaggg cacgatccgc gaggtgcgtc
     4741 cccctcagat cctcgagtac agccacttca gcccgctgac cgggttgccc gatgtacc
//
LOCUS       DASTXO010000070         9845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_77672, whole genome shotgun sequence.
ACCESSION   DASTXO010000070 DASTXO010000000
VERSION     DASTXO010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 9845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9845
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77672"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1338)
                     /locus_tag="VFU96_01330"
     CDS             complement(64..1338)
                     /locus_tag="VFU96_01330"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005150457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEU5111925.1"
                     /translation="MNIAIIGAGYVGLVTGGGLASLGHRVRLGEANAERVELLRNGGV
                     PIYEQGLPDLLSHATQRGLLSFHTSNKEAIGGAGLVFLALPTPEGEDGRADLKYVHAV
                     VDELANEVDEDTIFVVKSTVPPGTVASLRKRLADLGSQARIVSHPEFLREGKAVDDFL
                     EPDRIVVGAWEEADARAVADLYRSLDAPVVITDPTSAEMIKYASNAYLAARLTFVNTL
                     SNVCEAVGADILDVVRGLGMDHRVGPHFLQPGPGYGGSCFPKDTQALIAVAQDAGYDF
                     ELLRAVIEADEEQRRRVADKVRQAAGGGLRGRRVGMWGVAFKAGTDDVRESPAVRIAS
                     LLQADGAEVVAYDPEATTDLLTMAPDPIAAVTEADVLLIATEWPEFLTVDMAEVSKVM
                     RGHRVVDARNILDPKKVRSVGLDYWGLGRPGA"
     gene            complement(1371..2642)
                     /locus_tag="VFU96_01335"
     CDS             complement(1371..2642)
                     /locus_tag="VFU96_01335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU5111926.1"
                     /translation="MSSSSPRHLAPRRGLPKWLKITILGLSLATMLGPLAVFWALNTG
                     ENVLALAESDEEVVRELTAAIGDTLTFLVVGSDSRERLGDLAFFGPAGGERGDVIMLV
                     HLDQTTGVARILSIPRDLWVDIPGNGEGKINAAYSYGGPSLMVQTIRENLGIEVNHYV
                     EVDFVGFIEMVDELGGIEMTFPYPARDAKSGLSVDAGTQILDGEQALAFARSRRYQEY
                     QNDTWVSVDASDLGRTERQQEVVRAILTELKTPASIAEAGEIAGSMAEHMTIDAALAT
                     SSVASLAWDFRGLVTGSIDGQTLPVYADTVNGASVVMAAEPEASAMIDGFLSGVETIE
                     SAPLRIQVLNGNGIAGAASAMSERLAESGFEIAGVGNAEARDYATTTIVVPDGSTAGD
                     RIIGQIGFGVVQIGTVDNGYDAVVIVGADAS"
     gene            2732..3469
                     /locus_tag="VFU96_01340"
     CDS             2732..3469
                     /locus_tag="VFU96_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU5111927.1"
                     /translation="MAVLAHEGTHPIMYGGWPLPIGSSLRTQYLARPDVAGQFPVVLV
                     LPTLDGLSGFEKDICRMFARWGLAAIGVDSYRHKGDPHEAYEALSDRRALRDLDEIHE
                     FIVSDDVDWAVDGDLGILGLDVGGRFAIVGASTRQWVKSVAVAYTPLTGDEDREYQVA
                     SYLDNLPVPVLGLYGTDDELIASSTVDEAQRRNDHGQWLMYEGAGHSFLDIGADGYDA
                     DAAADAHPRLVEFFKTTLPAAIEVDLG"
     gene            complement(3466..4119)
                     /locus_tag="VFU96_01345"
     CDS             complement(3466..4119)
                     /locus_tag="VFU96_01345"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEU5111928.1"
                     /translation="MATPYLQMWAEEVPSTQDLARSRLGRLPVLVAAERQTAGRGRGG
                     AAWMSAPRAVAASLAFRPRVDDLRPFSLMAGVAAARGLEGVSLKWPNDLMLDGAKVGG
                     ILVERAEEVVVGMGVNLWWPGPPEGIGALHDDDPGGGLHARIAGLWAAELMRLIDGDG
                     WPIDEYRSYCSTLGRTISWDPEGQGNAVDVAEDGGLVVEVAGARQKLTSGAIRHLRH"
     gene            4164..5714
                     /locus_tag="VFU96_01350"
     CDS             4164..5714
                     /locus_tag="VFU96_01350"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006053919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEU5111929.1"
                     /translation="MGTEARIERLRELREEALHAGSQRAIARQHEQGKLTARERLDAL
                     LDKGSFQEIDQFVRHQSSGFGIEDKRPLGDAVVTGHGTIDGRPVFVFAEDFTVFGGSL
                     SKVVADKICKVLDMALDTGTPVIGLKDSGGARIQEGIASLDGYGRIFERNVRASGVIP
                     QISVIMGPCAGGAVYSPALTDFVFQVDQTSHMFITGPDVIKAVTGEEVTMEELGGAVA
                     HASKSGVTHFVYPSGIDALEAVRYLMSYLPRNNMELPPYFAPTDRPDRIDDSLDSTIP
                     DSANQPYDMVELVETILDGGEFLEVHEMWAKNIVVGFGRLDGHTVGLVANQPAILAGT
                     LDIEASEKAARFVRFCDAFNIPIITFVDVPGFLPGVEQEHHGIIRHGAKLLYAYCEAT
                     VPRITVITRKAYGGAYLVMNARGIRADLVFAWPSAEIAVMGAQGAVNIIHRREISATK
                     EPERVRSDLIADYEERFNNPYVAAELGLVDEVIEPRETRYKLIRALEVLRTKRETLPP
                     KKHGNGPQ"
     gene            5714..7441
                     /locus_tag="VFU96_01355"
     CDS             5714..7441
                     /locus_tag="VFU96_01355"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU5111930.1"
                     /translation="MKSLLVANRGEIAVRVIRTAREMGIRTIAVYSELDRDALHVDLA
                     DEAWNAGPAPAADSYLNQQKILQIAHDSGAEAIHPGYGFLSENARFAGAVTDAGIVWV
                     GPPASAIEAMGDKITSRRNAEKFGVPTVPGVTEPVTSVRQVATLAEQFGYPVAIKAAH
                     GGGGKGLRVVRGPDELDDAFEGARREADAYFGNPEVYVEKYLEQPRHVEAQILYDSQG
                     KGVFLGERDCSVQRRHQKLIEETPSPGLTVKQRRALGRAALAAGKSCGYVNAGTVEFL
                     VDRAGEFYFLEMNTRLQVEHTITEMVTGHDLVEWQLRIATGEELSIDAVEPRGHAIEF
                     RINAEDPYNQFLPTPGQVVEWQEPAGPGVRVDSWIRPGTNVSQYYDNLMAKLVVWAPD
                     RARAIQKGRRALEEFRVTGVASTIPAHLAVLNHPDFINGTHHTKWMEENVDLGGGDPD
                     ATPSLPEEEILSRRDITVEVGGRRFAVSYWAPEPLTGSIRRRPPKLQLTGPLSASDGM
                     VTAPMQGTIVKVHVAAGAAVSEGDPICVLEAMKMENEVRSPATGAVVDLRVQPGDTVT
                     PGQVIAIVK"
     gene            complement(7464..8822)
                     /locus_tag="VFU96_01360"
     CDS             complement(7464..8822)
                     /locus_tag="VFU96_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5111931.1"
                     /translation="MSDTRRHLVIIGGGPAGTQAATTAASKGGKVTLIEKEIVGGAAH
                     LWDCIPSKTMAASALRHSSVRNAAQLGLATEPTRVDTGVLAARIKAITGDINRNWLEL
                     LESQSVEVIYGEGRFVSDREVIVDTGSGERRVAFDRAMVSTGSSPRVPEWAPVDGEKV
                     LTTRDCYDMVDVPEHIVIIGSGVTGVEFTHIFESLGSKVSLVVSRRQILPHRDAEVAA
                     VLEEDFLERGVHLAIGARAEKIDLDGSGVSVFCDDGRVLRGSHALLAVGSIPLTEGIG
                     LDAAGVTAKDGYIEVDEYQRTSVPTIYAAGDVTGQMPLSSVASMQGRKIARHALDLPV
                     TPLDYSKVAEAIFTEPEIASVGLVDVEAAAQGRKVRTMKVPFAANARSVLQHFTRGFV
                     KLTSDPATGVVLGGTIVGHRASELIGVLTLAVQADVTVKELVETLMVHPSMTEAISDA
                     AE"
     gene            complement(8836..>9845)
                     /locus_tag="VFU96_01365"
     CDS             complement(8836..>9845)
                     /locus_tag="VFU96_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEU5111932.1"
                     /translation="TDVFAYTATELPQWNPISISGYHIREAGSTAAQELAFTIANGLA
                     YVQAAMDAGLDVDAFAPRLSFFWNGHNAFFEEIAKYRAGRRLWARLMRDDVGAKDPKS
                     WTMRFHTQTAGSTLTAQQPLNNVVRTTIQALAAVLGGTQSLHTNSYDEALGLPTDDSA
                     LLALRTQQVIAHESGVVDTVDPLGGSYFVEYLTDRLEEAARSLLARIEEMGGAVVAIE
                     EGWMQAQIEDAAYAEARRQADGSGVVVGVNRYSTGGGEPIPVLEVDPALEAEQRRRLS
                     GWRAGRDESAVESALGDLQEQAGGDGNLLAPMKVALHHGATVGEVSDRLRAVFGVYRP
                     S"
BASE COUNT         1779 a         3223 c         3133 g         1710 t
ORIGIN      
        1 gatctggtcc ggccacccga agaggacatg ttccggtgat ggcggacctg ggcccggggc
       61 cgtctatgcg cccggacggc ccagacccca atagtcgaga ccgaccgacc tgaccttttt
      121 cgggtccagg atgttgcgcg catcgaccac acggtgtccc cgcatcacct tggagacctc
      181 agccatgtcc accgtgagaa actccggcca ctcggtggcg atgagcaaca catctgcttc
      241 ggtcactgcc gcgatcggat ctggtgccat agtcagcaga tcggtcgtcg cctcggggtc
      301 gtatgccacc acctcggcgc catcggcctg gagcaaggac gcgatccgaa cggccggcga
      361 ctcgcggacg tcgtccgtcc ccgccttgaa ggcgacgccc cacatgccta cccggcgccc
      421 tcgcagacca ccgcccgcag cctgccgaac cttgtcggcg actcgccggc gctgctcctc
      481 gtcggcttcg atgacggccc gaagcagctc gaagtcgtag ccggcatcct gggccacggc
      541 gatcagggcc tgagtgtcct tcgggaagca ggagcccccg tatcccggcc cgggctggag
      601 gaagtgcggg ccgacacgat gatccattcc cagacctctg actacgtcga gtatgtcggc
      661 gccgaccgcc tcgcagacgt tggacagggt gttgacgaag gtgagcctcg cggccagata
      721 ggcgttcgag gcgtacttga tcatctcagc agatgtcgga tcggtgatga cgaccggcgc
      781 gtccaggctg cggtagagat cggcaacagc tcgtgcatct gcctcttccc atgcaccgac
      841 gacgattcga tccggctcga ggaaatcgtc taccgccttg ccctcccgca ggaactcggg
      901 atgggagacg atccgcgcct ggctccccag gtcggcgagc ctctttcgca gcgatgcaac
      961 cgtgcccgga gggacggttg acttcacgac gaagatcgtg tcctcatcta cctcgttggc
     1021 cagctcgtct acaacggcat ggacgtactt gaggtcagct cggccatctt ccccctcagg
     1081 ggtgggcaga gcgagaaaga ccaggccggc gccaccgatt gcctccttgt tggaggtgtg
     1141 aaaactgaga aggccgcgtt gggtcgcatg agacaggagg tcaggcaacc cctgctcata
     1201 tataggcacc cctccattgc gcaggagctc gactctttct gcattggcct cgccgagacg
     1261 cactcggtga ccaaggctgg cgagcccacc ccccgtgacg aggccgacgt agccggcacc
     1321 aatgattgcg atgttcatcg accccccatg gtattaggtg cgcgctgcga tcaggaagcg
     1381 tcagcaccga cgatcacaac ggcgtcatag ccgttatcga ccgtaccgat ctgaactaca
     1441 ccgaaaccga tctgcccgat gatccgatcc cctgcggtgc ttccgtcggg gacgacgatc
     1501 gtggtcgtgg catagtccct cgcctccgcg ttgccgaccc cggcgatctc aaagcccgac
     1561 tcggccagcc tttccgacat ggccgaggca gcgcctgcaa tgccattgcc gttcaacacc
     1621 tggattcgaa gcggtgcgga ttcgattgtc tcgacgccgg aaaggaaccc atcgatcatc
     1681 gccgacgcct ccggctccgc ggccatgacc acggaggcgc cattcaccgt atcggcgtac
     1741 acgggcaggg tctggccgtc gatgctcccg gtcaccagac cacggaagtc ccaggccagt
     1801 gaggcgacgg aggaggtggc gagggcggca tcgatagtca tgtgttccgc catggagccc
     1861 gcgatctctc ccgcctccgc aatcgaggcc ggggtcttca actcggtgag gatggcgcgg
     1921 accacttcct gttgtcgctc cgtccgtccc aggtcgctgg catcaacgct cacccacgtg
     1981 tcgttctgat actcctgata ccttcgggag cgggcgaagg cgagtgcttg ctcgccgtcg
     2041 agtatttgtg tcccggcgtc gacgctgagc ccagacttgg catcacgcgc cgggtagggg
     2101 aaggtcatct cgatcccacc caactcgtcg accatctcga tgaacccgac gaagtcgacc
     2161 tcgacgtagt gattgacttc gatcccgaga ttctcccgga tggtctgaac catcagcgaa
     2221 ggcccgccgt atgagtaggc cgcgttgatc ttgccctcac cattgcccgg gatgtccacc
     2281 cagagatccc ggggaatcga gagtatccgc gccacaccgg tcgtttggtc gagatggacg
     2341 agcatgatca catcgccccg ctccccaccc gccgggccga agaaggcgag gtcacccagg
     2401 cgttcgcgcg agtcgctccc gacaaccagg aacgtcaatg tgtcgccgat ggccgcagtg
     2461 agctctctga cgacctcctc gtcgctctcc gccagcgcga gcacattctc gcctgtgttc
     2521 aaggcccaga agaccgccag cgggccgagc atggtcgcca acgagaggcc gaggatcgtg
     2581 atcttcagcc atttgggcag cccgcgacgg ggcgcaaggt gtcgtgggga ggatgaggac
     2641 atcggcggat agagacgttc tcaggctagg caggggttcc tcgaagttct ggcaatcgcc
     2701 ggatggcccg gcgggcctaa tctgcccgcc catggcggta ctggcgcacg aaggaaccca
     2761 tccgatcatg tacgggggct ggcccttgcc catcgggtca agtctccgca cgcagtacct
     2821 ggcccggccg gacgtcgcgg gtcagttccc cgtggtgctc gtgctcccga ctctcgatgg
     2881 cttgtccgga ttcgagaagg acatctgccg catgttcgcg cgctggggac tcgcggccat
     2941 cggggtcgac tcatatcgcc acaagggtga cccccatgag gcatacgaag cgctatccga
     3001 ccgtcgcgcg ctgcgggacc tcgacgagat ccacgagttc atcgtgtccg atgacgtcga
     3061 ctgggcggtc gacggtgatc tagggatcct cggcctcgac gtcgggggac gcttcgcaat
     3121 cgtgggcgcg tcgacccggc agtgggtcaa gtcggtggca gtggcctata cgcccctcac
     3181 cggcgacgaa gaccgtgagt accaggtggc ctcctatctc gacaacctcc cggtgcctgt
     3241 cctcggactc tacgggaccg atgacgagct gatcgcctcc tccacggtcg atgaggcaca
     3301 acgtaggaac gaccatggcc agtggctcat gtacgaaggg gcagggcatt cattcctcga
     3361 tatcggggcc gatggctacg acgcagacgc tgccgctgac gcccacccac gcctcgtcga
     3421 gttcttcaag acgacactgc cggccgcgat cgaggtggat ctcggctagt ggcgcaggtg
     3481 cctgatcgcg cctgaggtca acttctggcg ggcgccggcg acctcgacta ctagaccccc
     3541 gtcctcggcg acgtcgaccg cgttcccttg gccctcggga tcccacgaga tcgttcggcc
     3601 cagcgtggag cagtacgacc tgtactcgtc gatcggccat ccgtcaccgt cgatgagacg
     3661 catcaattcc gccgcccaca ggccggcgat ccgagcgtgc aggccgccgc cgggatcgtc
     3721 atcgtggaga gctccgatcc cttcgggagg tccgggccac caaaggttga cccccattcc
     3781 gacgactacc tcctcggcgc gctcgacgag gatccctccc actttggcgc catccaacat
     3841 caagtcgttg ggccacttca ggctgacacc ttccaggcct cgggcggccg ccacgcctgc
     3901 catcagcgag aagggccgaa ggtcgtcaac acggggccgg aaggcgagcg aggccgcgac
     3961 ggcccgaggc gcggacatcc aagcagcgcc tcccctccct ctaccggccg tttggcgctc
     4021 ggcggcgacg agtaccggca gacgaccgag tctggagcgg gcgaggtcct gtgtggatgg
     4081 gacctcctcc gcccacattt gcaaataggg tgtagccatc tcgtctcctg acgagtcgga
     4141 ttgtgtgagg atacggagga gtcttgggga ccgaggcgcg aatcgagcgg ctcagagagc
     4201 tacgcgaaga ggccctgcat gccgggagtc agcgagccat cgcccgccag cacgagcagg
     4261 ggaaactgac cgcccgagag cggctcgacg cattgctcga caagggctcc ttccaggaaa
     4321 tcgatcagtt cgtccggcac cagtcgtcgg gcttcggcat cgaggacaag cgccctctcg
     4381 gcgacgccgt cgtcacggga cacggcacca tcgatggccg accggtcttt gtcttcgccg
     4441 aggatttcac cgtttttggc ggcagcctga gcaaggttgt cgccgacaag atctgcaagg
     4501 tgctcgacat ggccctcgac accgggaccc cggtgatcgg gctcaaggac tcgggtggag
     4561 cccgcatcca ggaagggatc gcctctctcg acgggtacgg gcgcatcttc gagcgcaatg
     4621 tgagagcgtc aggggtcatt ccccagatct cggtaatcat gggaccgtgc gccggagggg
     4681 cggtctactc ccccgccctc accgacttcg tgttccaggt cgaccagacc agccacatgt
     4741 tcatcaccgg gcctgacgtc ataaaggcag tgaccggcga ggaggtgacc atggaggagc
     4801 tgggcggggc cgtcgctcac gcgtcgaaat ccggcgtcac ccatttcgtc tatccgagcg
     4861 gcatcgacgc actcgaagcg gtgcggtacc tcatgtcgta cctgccgcgc aacaacatgg
     4921 agcttccccc ttacttcgcc cccaccgatc gccccgatcg catcgacgac tccctggatt
     4981 ccacgatccc cgattctgcg aaccagccat acgacatggt ggagctcgtt gagaccatcc
     5041 tcgatggggg tgagttcctc gaggtgcacg agatgtgggc gaagaacatc gtcgtgggat
     5101 tcgggcgcct ggacgggcac acagtgggct tggtggccaa ccagcctgca atcctggcag
     5161 gcacactcga catcgaggcg tcagagaagg cggctcgatt cgtccgtttc tgcgacgcct
     5221 tcaacatccc gataatcacc tttgtcgacg tccccggctt tctcccgggt gtcgagcagg
     5281 aacatcacgg gataatccgc cacggggcca agctcttgta cgcgtattgc gaggcgacag
     5341 tgccccgaat cacggtcatc acccggaagg catacggcgg cgcctacctg gtcatgaacg
     5401 cccgtgggat acgggcggac ctggtcttcg cctggccatc ggccgagatc gcggtgatgg
     5461 gcgcacaggg tgcggtgaac atcatccatc gccgggagat cagcgcgacc aaagagcctg
     5521 agagggtccg ctcggatctc attgccgact acgaggagag gttcaacaac ccgtacgtcg
     5581 cagccgagct cgggctcgtc gacgaggtga tcgagcctcg tgagactcgt tacaagctga
     5641 tccgagctct cgaggtactt cgcaccaagc gagagaccct ccccccgaag aagcacggaa
     5701 acggccctca gtgatgaaga gcctcctcgt cgccaatcgc ggcgagatcg cggtccgggt
     5761 gatccgcacg gcccgagaga tggggattcg caccatcgcc gtgtattcgg aactggatcg
     5821 cgacgccctt catgtcgacc tcgctgacga ggcatggaat gccggccccg cccctgcggc
     5881 cgacagctat ttgaaccaac agaagatcct ccagatcgcc catgattcgg gcgccgaggc
     5941 gatccacccc ggttacgggt tcctttccga gaatgcccgg ttcgcaggag cggtcacgga
     6001 cgcagggatc gtgtgggtcg gtcccccggc cagtgccatc gaggccatgg gagacaagat
     6061 cacctcgaga cgcaatgccg agaagtttgg cgtgccgact gtgcccggcg tcaccgagcc
     6121 ggtcacctcg gtccggcaag tcgcaaccct cgccgagcag ttcggctacc cggtggcgat
     6181 caaggccgct catggcggtg gcggcaaggg tttgcgggtg gttcgcgggc ccgacgagct
     6241 cgatgacgct ttcgaaggtg cccgacgtga ggcagatgcc tatttcggga accccgaggt
     6301 ctacgtcgag aagtatctgg aacagccccg acacgtcgaa gcccagatcc tctacgacag
     6361 tcagggcaag ggggtcttcc tcggggagcg ggactgctcg gtgcagcgac gtcatcagaa
     6421 actgatcgaa gagacaccgt cgcctggact caccgtgaaa caacgccgag cactgggccg
     6481 agccgcgttg gccgcgggca agtcctgcgg atatgtcaat gccgggacag tggagtttct
     6541 ggtcgaccgc gccggcgagt tctacttcct cgagatgaac actcgtctcc aggtcgagca
     6601 cacgatcacg gagatggtca ccggtcacga cctggtcgag tggcagctca ggattgcgac
     6661 cggagaggag ctgagcatcg atgccgtcga gccgagaggg cacgccatcg agttccgcat
     6721 caatgccgag gacccctaca accaatttct ccccacgcca gggcaggtgg tcgagtggca
     6781 ggagccggcc gggccggggg tcagggtcga ctcgtggatc agacccggga ccaatgtctc
     6841 ccagtactac gacaatctga tggccaaact cgtcgtctgg gcgccggatc gtgctcgggc
     6901 cattcagaag ggcagacggg cattggagga gttccgggtg accggggttg cgtcgacgat
     6961 cccggcccat ttggccgtcc tcaatcaccc cgacttcatc aacgggaccc atcacaccaa
     7021 gtggatggag gagaacgtgg acctcggagg gggcgatccc gacgccaccc cctctctccc
     7081 cgaggaggag atcctgtctc gacgagacat aaccgtcgaa gtcgggggtc gtcgctttgc
     7141 agtgagctat tgggcgccgg agccgttgac cggcagcatc cgcaggcggc ctccgaaact
     7201 ccagctgacc ggccccctct ctgcgtccga tggcatggtc accgcaccga tgcagggcac
     7261 gatcgtcaag gtccatgtcg ccgctggggc ggcggtgagc gagggcgacc cgatatgcgt
     7321 gctcgaggcc atgaagatgg agaacgaggt ccgctcaccg gctaccggcg cagtcgtcga
     7381 cctcagggtc cagccgggcg acaccgtgac cccgggtcag gtcatcgcca tcgtgaagta
     7441 agaccccgcc ggacagtcag tgcctattcg gcggcgtcgg agatggcctc cgtcatcgag
     7501 gggtggacca tcagcgtctc aacgagctct ttgacggtga cgtctgcctg gacggccagg
     7561 gtgaggacac cgatgagctc cgaggcgcgg tggccgacga tggtcccgcc caggaccacc
     7621 ccggtcgccg ggtcggaggt gagtttcacg aagcccctcg tgaaatgctg gaggacggaa
     7681 cgggcattgg ccgcgaaggg caccttcatc gtccgcacct tccgcccctg ggccgcggct
     7741 tcgacatcga cgagaccgac cgaggcgatc tcgggctcgg tgaatatcgc ctcggcgacc
     7801 ttggagtaat cgagaggtgt caccggtaga tccagggcgt ggcgagcgat cttgcggcct
     7861 tgcatcgaag ctaccgatga cagcggcatc tgaccggtca cgtccccggc ggcgtagatg
     7921 gttgggaccg acgtccgttg gtactcatcg acctcgatgt agccatcctt ggctgtgact
     7981 ccggcggcgt cgagaccgat gccctcggtg agagggatgg agccgaccgc gaggagcgca
     8041 tgcgaaccgc gcagcacccg accgtcatca cagaagaccg agacccctga cccgtccagg
     8101 tcgattttct cggctctggc cccgatggcg agatgcacgc ctcgctcgag gaaatcctcc
     8161 tcgagcactg ccgcgacctc ggcgtcgcgg tggggaagga tctgacgacg ggaaacgacg
     8221 agactcacct ttgatcccag gctctcgaag atgtgggtga attccacccc cgtgacaccg
     8281 gatccgatga tgacgatgtg ctcggggacg tcgaccatgt cgtaacagtc ccgtgtcgtg
     8341 agcacctttt ctccgtcgac cggtgcccac tcggggacgc ggggcgacga accggtggag
     8401 accattgccc gatcgaaagc gacgcgacgt tcgcccgaac cggtgtcgac gatgacctcc
     8461 cgatccgaga cgaagcggcc ctcgccgtag atcacctcca ctgactggga ctcgagcagt
     8521 tccaaccagt tgcgattgat gtctccggtt atggccttga tccgggccgc caggactccc
     8581 gtgtcgactc gggtgggctc ggtggcaagc ccgagctggg cggcgttgcg aaccgatgaa
     8641 tggcgcaagg cgctggcagc catggtcttc gacgggatgc agtcccagag atgggcggcg
     8701 ccgccgacga tctccttttc gatcagcgtg acttttcccc ccttcgatgc cgccgtggtg
     8761 gcagcctggg tcccggccgg tccaccgccg atgatgacca gatgtctcct ggtgtcgctc
     8821 atgtcgtttc catcctcatg agggacggta gacaccgaac acggcgcgca ggcgatcgga
     8881 cacttcgccg acggtggccc cgtgatgtag cgcgaccttc atcggggcga ggaggttgcc
     8941 gtcccctccg gcctgctcct ggaggtcgcc gagagccgac tccacggcgg actcgtcccg
     9001 acccgcccgc catccggaca atcttcggcg ctgctccgcc tcgagtgcgg ggtcgacctc
     9061 gagtacgggg atcggctctc cgccgccggt ggaatagcgg ttcactccga cgacgacccc
     9121 cgatccgtcg gcctgacgtc gcgcctctgc atatgccgcg tcctcgatct gggcctgcat
     9181 ccagccctct tcgatggcaa ccaccgcccc acccatctcc tcgatccgtg ccagcagaga
     9241 acgggcagct tcctcgagtc ggtctgtcag gtattcgacg aaataggacc cccccagggg
     9301 gtcgaccgtg tcgaccacgc cggactcatg agcaatcacc tgctgggtcc gcagtgccaa
     9361 caacgccgag tcatccgtgg gcaggcccag tgcctcgtcg tacgagttcg tgtgaagcga
     9421 ctgtgtgccg ccgaggaccg cggccagcgc ctgaatcgtg gtccgaacca cgttgttcag
     9481 cggctgctgg gcggtgaggg tggagcccgc ggtctgagtg tggaatcgca tcgtccagct
     9541 cttcgggtcc ttggcgccga cgtcgtcccg catcagcctc gcccataggc gccggccggc
     9601 gcggtacttg gcgatctcct cgaaaaacgc gttgtgcccg ttccagaaga acgaaaggcg
     9661 aggggcgaat gcgtccacat cgagacccgc gtccatcgcg gcctgcacgt aggcgagccc
     9721 gttggcaatg gtgaatgcca gctcctgggc ggcagtcgag cccgcctcgc ggatgtggta
     9781 gccgctgatc gagatcgggt tccattgcgg tagctcggtg gcggtatatg cgaacacgtc
     9841 ggtga
//
LOCUS       DASTXO010000071         2396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_409362, whole genome shotgun sequence.
ACCESSION   DASTXO010000071 DASTXO010000000
VERSION     DASTXO010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409362"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..897
                     /locus_tag="VFU96_01370"
     CDS             220..897
                     /locus_tag="VFU96_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111933.1"
                     /translation="MTPAQRLCASRASAAKRYTEIFIIGGNPLHLVGGIYNTIGLNDP
                     DETGDTTPQEVLDGVDIDAVVEEYDAMGAFKNGPRLWCLDWAEIMSGQVRDFNGLQAR
                     WVMWLDVPKEMRKHESVAYKQIHGKRDTQLGIDAGSPAFLLDDPEGNSWCMKSAGLIV
                     DPNQTYESLKNLGDRLKPAEGWNFRTVVLDRDLVLMADDGAVQITQDELGNTYDRVGG
                     PYSNYKP"
     gene            complement(1014..1286)
                     /locus_tag="VFU96_01375"
     CDS             complement(1014..1286)
                     /locus_tag="VFU96_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111934.1"
                     /translation="MEDREGMAAEAVAIYEELFETFKGRLSSDPKWSESRMHELMDAT
                     FDEVRGLNLPGVSTSDEVIVRASTALSQLMADAVLQTIAVYLARRS"
     gene            1649..2029
                     /locus_tag="VFU96_01380"
     CDS             1649..2029
                     /locus_tag="VFU96_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5111935.1"
                     /translation="MSRDLLTVAPEDTLGEAAEKMAGRGVGAALVMDFGRLIGILTER
                     DLLAAVASRVHTSEAQVREWMTENPVTVPEAMPIERAASVMVTRGFRHLPIVDGERTI
                     GIVSLRDVVRSVVRVFPEYEDETE"
BASE COUNT          448 a          763 c          784 g          401 t
ORIGIN      
        1 cccggcccgg gcggccccgg tcgtcccggc cccggcccag gcggccccgg tcgtcccggc
       61 ccgggcggcc ccggtcgtcc cggccccgga cgttgatcat tgacagggag acgtcgggaa
      121 tagccatcta aagcgggaag ggccggctag gggggcacca ggccgtgatc gacaaaagga
      181 ggagggtgga cgtgaaactt cacgcgaagc tgccagagga tgacgccggc gcaacgctta
      241 tgcgcttcga gggcgtccgc ggccaagcgg tacacggaga tcttcatcat cggaggcaac
      301 cccctccacc tggtcggcgg gatctacaac acgatcgggc tgaacgaccc cgacgaaacc
      361 ggagacacga ccccccagga ggtgctcgac ggagtcgaca tcgacgccgt ggtggaagag
      421 tacgacgcca tgggcgcgtt caagaacggc cccaggctct ggtgcctgga ctgggcggag
      481 atcatgagcg ggcaggtgcg cgacttcaac ggcctgcaag cccggtgggt gatgtggctg
      541 gacgtgccaa aggagatgcg caagcacgag agcgtcgcct acaagcagat ccacggtaag
      601 cgcgacacgc agctcggcat cgatgcgggt tcaccggcgt tcctcctcga cgacccagag
      661 ggcaactcgt ggtgcatgaa gtccgccggc ctaatcgtcg acccgaacca gacctacgag
      721 agcctgaaga acctcggcga ccggctgaag cccgcggagg gctggaactt tcgaaccgtg
      781 gtgctcgatc gggacttggt tctcatggcg gacgacggcg ctgtccagat cacccaagac
      841 gagcttggga acacgtacga ccgggttggt ggcccataca gcaactacaa gccgtgacca
      901 cccggcacct caccgcactt tcgacttcgg ccgcggatca gggttcggca ccctcacccc
      961 gtccacgtag atccacttcg gaacgacccg acgcgcatgg ctctgacgcc ttcctaggag
     1021 cgacgagcca agtagacggc gatggtctgc aggaccgcat cggccatcag ttgagagagc
     1081 gcggtcgatg cgcgcacgat gacctcgtcc gaggtgctca ccccggggag attcaaaccc
     1141 ctgacttcgt cgaacgtcgc gtccatcagc tcgtgcatcc gactctcgct ccactttgga
     1201 tcgcttgaga ggcggccctt gaacgtctcg aagagctcct cgtaaatggc aaccgcctcg
     1261 gcggccatcc cctcacggtc ttccatcgga tctcctttcc ccccgtttcg ggcctctgcc
     1321 cgggccaacc taccgccggt cgcctcccgg cgctgcagac gccaggcgac aatgagggcg
     1381 agagttcgac gaacgcgtgc ggatcctcga agcgtgtacc cgatcggggc cagcgaccag
     1441 gaggagtccc cggttgcgtt gcatgcgccc atctatccac ctcgcccaca cgtcctgatc
     1501 ttgccgcctt cctccgatct ccattctggc caggacattc ccttctttcg ttccttggcg
     1561 agatactggc acgcgtgggc ggtgccatcg aaaacgcgaa ttgtccgagt tatgctcgct
     1621 tcggatgcga gggttgcttc gcgatgtaat gtcgcgcgat ctcctgacgg tcgctcccga
     1681 ggacacgctc ggcgaagcgg cagagaagat ggccggtaga ggcgtagggg ccgctctcgt
     1741 catggacttc ggccgactca tcgggatcct gaccgagcgc gatctcctgg cggcggtcgc
     1801 gagcagggtc cacacgagtg aggcgcaagt cagggagtgg atgacagaaa atcccgtgac
     1861 ggttcccgag gcgatgccga tcgaaagagc cgcgagcgtc atggtgacgc gcggctttcg
     1921 ccacctgcca atcgtcgacg gtgagcggac catcgggatc gtcagcctcc gcgacgtcgt
     1981 ccggtccgtt gtccgcgttt tcccggagta cgaagacgag acggagtagc cttacgcacc
     2041 ggcagggctt gaagcgacct ctgccgagct cttttcaacg aagcagggcg acgtgacaat
     2101 cacccacgac taggacacat ttgctggtcg cgctcaagag ggcggcttgc catcgggtag
     2161 ccgccttcgc ctcatccggc gtcggcgcaa gtaaagacgc gtctagccga gaagcagtac
     2221 gagcacgagc gcgaggatga tgatcgcggc ggcggacgtg gacgagatcg gctggctgat
     2281 ccggtggcta actgggggct tgacgagtcg tctgcagggg cggatagtgg cggctctggc
     2341 gtcctttccg ggaggggtca catggggtac aggtctttgc cagtgctgat ggcggc
//
LOCUS       DASTXO010000072         2095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_335612, whole genome shotgun sequence.
ACCESSION   DASTXO010000072 DASTXO010000000
VERSION     DASTXO010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335612"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2055)
                     /locus_tag="VFU96_01385"
     CDS             complement(<1..2055)
                     /locus_tag="VFU96_01385"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5111936.1"
                     /translation="MVRSVQEAVDERSVTFLATDIATNGGKIILTAGVPTTTGRDEEE
                     MLLALRQVISGGPALPLSVGASWGRIFAGEVGTPYRRTYTVMGDTVNLAARLMARAPA
                     GEIYATNEVVAGSRTTFAVDHLEPFLVKGKKLPVTALSVGDPHGTKSQREAGGLPLIG
                     RDRELALLNSAWEKARAGNGQMVELAADQGMGKSRLLDEFLLASQPERVVRAECRLYQ
                     SATSYFPFRELLGEAWSLGGLGPEAAEKALAELVDTVAPELRPWLSLIGLPLGLEIAD
                     SVEASQLEDQFRPARTIAAVGALLEATITDPVMFVVEDTHWMDEPSRGLLAGLVSGLE
                     RDPWMFVLTRSLGEQGFVAPAMQSVTRIELHPLGLDEAKNLIFSATEDAPISQEQVEL
                     LARQADGNPLFLIELLQALRHTGSLEEIPQSVEAMIATRIDTLPQIDRNVLRRLSVLG
                     TGFRLETVSAVLGEEGESPQDHSHSVERLSGFVSIDESGWVEFHHSLIRDVAYDGLPF
                     RTRERLHERVGDATFAGCRGRPEEFAEILSFHYFHAKRWSRTWRFSRMAGERAREIYA
                     NHEAADFYERALSAARHLDWVTPADRAEVLTALAGVLYEAGLFDEAIASLREATRVID
                     HDLVARADLHRLLARAYQKLGKHSLGLRETAIGLNVLAPSDQVDARRARARLKALRAG
                     ILS"
BASE COUNT          370 a          688 c          669 g          368 t
ORIGIN      
        1 tgagaggatc ccggcgcgca gcgccttgag tcgggctcgg gccctcctcg cgtcgacctg
       61 atcagacgga gcgagcacgt tgaggccgat tgcagtctcg cggagcccca gggaatgctt
      121 gcccagtttt tggtaggcgc gggccaacaa tcggtgcaga tcggcacgag ccaccaggtc
      181 atggtcgatc acccgggtcg cctcacggag ggaggctatg gcctcatcga acagaccggc
      241 ctcgtagaga acgcccgcca gagctgtgag cacctctgcc cgatcggcgg gcgtgaccca
      301 gtcgaggtgt cgggccgcgc tcagtgcccg ctcgtagaag tcggccgcct cgtgattggc
      361 atagatctct cgggctcttt cgccagccat ccgggaaaag cgccacgtgc ggctccatcg
      421 tttggcatgg aagtagtgga acgagaggat ctcggcaaac tcctccgggc gacctcgaca
      481 gccggcgaat gtggcgtcgc cgacgcgctc atgaagtcgc tccctggtcc tgaacggcag
      541 cccgtcatag gcaacgtcgc ggatcagcga gtgatggaac tcgacccatc ccgattcgtc
      601 gatggacacg aacccggaga gcctctcgac ggagtgggag tggtcctgcg ggctctcacc
      661 ctcctcgccc agcaccgccg acacggtttc gaggcgaaat ccggtgccga ggacggaaag
      721 ccggcgcagg acgttccggt ctatttgagg gagtgtgtcg attcgagtgg caatcatggc
      781 ctccacgctc tgcgggatct cctcgagcga cccggtgtgg cggagggcct gcagcaactc
      841 gatcaaaaag aggggattcc catctgcctg gcgggccagc aactccacct gttcctggga
      901 tatgggtgcg tcctcggtgg cgctgaagat gagattcttg gcctcatcca atcccagggg
      961 atgcaactcg atcctggtca cagattgcat ggccggggcg acgaacccct gttcgccgag
     1021 tgatcgcgtc agcacgaaca tccaaggatc acgttccaga ccggaaacga gacctgcgag
     1081 gaggccccgc gaaggttcgt ccatccagtg cgtgtcttcg accacgaaca tcaccgggtc
     1141 ggtgatggtc gcctcgagca atgcgcctac cgccgcgatg gtccgcgccg gccgaaactg
     1201 gtcttcgagt tgtgacgcct ccacggagtc ggcgatttcc agcccgagcg gtaatccgat
     1261 cagggaaagc cacgggcgca attcgggcgc gacggtgtcg acgagctccg ccaaggcttt
     1321 ctcggcggct tccggtccca gacctccgag gctccaagcc tcgccgagca gctcgcggaa
     1381 tgggaagtac gacgtcgccg actgatagag gcggcactcc gcgcgaacga ccctttctgg
     1441 ctggcttgcg agcaggaact catcgagcag acggctcttt cccatcccct ggtccgcagc
     1501 gagctccacc atctgcccgt ttccggctcg cgccttctcc caggctgagt tgagcagcgc
     1561 cagctcccga tcacgtccga tgagggggag accgccggct tcccgctggc tcttggtccc
     1621 atgcgggtcc cccaccgaca aggcggtgac cggaagcttc ttgcccttga ccaagaacgg
     1681 ctcgaggtga tcgaccgcga aggtggttcg tgacccggcg accacctcgt ttgtcgcgta
     1741 gatctcacct gctggtgctc tcgccatcag gcgggcggca aggttcaccg tgtcccccat
     1801 caccgtatag gtcctgcgat agggagtgcc gacctcgccg gcgaatatcc ggccccacga
     1861 cgcgccgacc gagaggggaa gggctggccc gcccgagatc acctggcgca gggcgagcaa
     1921 catctcctct tcatcgcggc cggtcgtcgt ggggacaccg gcggtgagga tgatcttgcc
     1981 tccgttggtc gcgatatcgg tcgccaggaa tgtcaccgaa cgctcgtcga ctgcttcctg
     2041 gactgaccgg accaaggcgt ccaacctctg cgcggcgacc tcggctccat cgcgc
//
LOCUS       DASTXO010000073         3802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1183114, whole genome shotgun sequence.
ACCESSION   DASTXO010000073 DASTXO010000000
VERSION     DASTXO010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183114"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VFU96_01390"
     CDS             <1..432
                     /locus_tag="VFU96_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5111937.1"
                     /translation="PEGGEGSAYFLGLWEYHGVVQEPAWPLPDDPLYPEVVLRGLTTM
                     VPGFDAYRDRLPQSVVDGGYYTKTTENRPLVGPAGPSGFNLLAGLSGFGVMVAAGAAD
                     LLARHVTGAELPAYSDAFLLARYDNPEYLASIAGPLSSGQL"
     gene            complement(436..807)
                     /locus_tag="VFU96_01395"
     CDS             complement(436..807)
                     /locus_tag="VFU96_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009307303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5111938.1"
                     /translation="MFEKTKAFSGFSVDDIEEARQFYRGTLGLDVTEENGMLAIHIAD
                     GGDIMAYPKEDHTPATFTILNFPVDDIDQAVDDLGARGVSFERYPQFEQDEKGIVRGD
                     EGPPIAWFLDPAGNVLSVLEG"
     gene            complement(1063..2058)
                     /locus_tag="VFU96_01400"
     CDS             complement(1063..2058)
                     /locus_tag="VFU96_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009308994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5111939.1"
                     /translation="MSESTATEPRSDTPSLEDLEQYRRELNAYAYRMLGSSFEAEDAV
                     QDSLIRAWRASDRFEGRSSLRTWLYRITTNVCLDMLEGRERRARPMDLGPASTADTML
                     PAPLPEVTWIEPAPDSIILAPSPDPAEAAIARESVRLAFITALQHLPARQRAVLILRE
                     VLRWKADEVAELLETTVASVNSALQRARVTLSAIEPARSDLYRPLDEEQQALLSRYVD
                     AFERYDLDSLVALLHDDVVQSMPPYDLWLRGHAAIRGWFLGTGIGCRGSVVVPTEAAN
                     GLPAFGQYKPGPDGGLVPWSLQVLEISDGRIAGLNFFLDTNRLFPLFGLPLVYEG"
     gene            2222..3604
                     /locus_tag="VFU96_01405"
     CDS             2222..3604
                     /locus_tag="VFU96_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5111940.1"
                     /translation="MHSPSTTNELSIRELREALDGEVITPDDHSYDEARHVFYGIDRR
                     PAVIIRPTNADEVAYVVSVARDSGAELAIRSGGHSIAGYGSSDGGITLDLSEMKSVRV
                     DSEHRTAWAQTGLTAGELTTELGKYGLAVGFGDTGSVGIGGITLGGGVGFLSRKFGLT
                     IDSLLAAELVTADGKVLQVDTESHPDLFWAIRGGSGNFGVATSFQYRLHEVDQMVGGM
                     LILPATPETIAGFVELADSAPDELSTIANVMKAPPMPFLPEELHGQVILFTLLVYAGD
                     PETGEEVIAPFRELARPLSDMVEPGPYAAIYEDEEEGPPPGVFAVRNLFVDKIDRAAA
                     ETIIEHVTASTAAMAVAQIRVLGGAIAQVPEETTAYAHRREPIMLNIASMFEQPDEAA
                     THQVWVDRLATALGDKGTAYVNFIGDEGESRVRQAYPGQTWERLRQVKQRYDPTNLFR
                     VNQNIPPAGD"
BASE COUNT          685 a         1199 c         1235 g          683 t
ORIGIN      
        1 cccgaagggg gggagggctc cgcctatttc cttggtctct gggagtacca cggcgtggtt
       61 caggagcctg cctggcctct gccagacgac cctctctatc cagaagtggt gctgcgcggg
      121 ctgacgacga tggtccctgg gttcgacgcg taccgggacc gtctccccca atcggtcgtc
      181 gacggcgggt attacaccaa gaccacggag aaccgccccc tcgttgggcc tgccggcccg
      241 tcgggtttca atctgttggc cggactgtcg gggttcgggg tcatggtcgc cgccggggcg
      301 gccgacctgc tcgccagaca tgtgaccggc gccgagctgc cggcctactc cgatgcgttc
      361 ttgctggcgc gatacgacaa ccccgagtac ttggcttcga tcgcgggccc actcagctcg
      421 ggccagctct gacgctcagc cctcgaggac ggagaggacg tttccggcgg gatcgaggaa
      481 ccaggcaatc gggggaccct cgtcaccacg cacgatgccc ttctcgtcct gctcgaactg
      541 cggataccgc tcgaacgaca cgccccgcgc tccgaggtcg tccactgcct ggtctatatc
      601 gtcgaccggg aagttgagga tggtgaacgt cgccggggtg tggtcctctt tcggataggc
      661 catgatgtcc ccgccgtcgg cgatgtggat ggcaagcatt ccgttctcct cggtgacgtc
      721 gagacccaac gtccccctgt agaactggcg cgcctcttcg atgtcgtcga ccgaaaagcc
      781 gctgaaagcc ttggtttttt cgaacacggc cacctcctgt ggtccggatg agatgagttt
      841 tcccgtcgga gggagtctct aatcgcattg gacggagaaa tcgatcccgg acgacattcc
      901 ctggtctgtg acgtcggccg agtgacgacg cccgatctgg ccacgatcga ggccttgtgt
      961 cgcttgcgtc tggccgttgc cgaccgcgga tatgcggtcg agctgcgcgg tgcttcgcca
     1021 ggcctccgcg agttgctcgg cctgtgtggt ctcgacgggg ccctacccct cgtagacgag
     1081 gggcagcccg aacagaggga acaaccggtt ggtgtcgagg aagaagttga gcccagcgat
     1141 ccggccatct gagatctcca gcacctggag cgaccagggt acaagtccgc cgtcaggtcc
     1201 gggcttgtac tgaccgaacg cgggcaggcc gtttgccgct tccgtgggca ccacgaccga
     1261 gccgcgacaa cctatgcctg tccccaggaa ccagcccctg atcgcggcgt gaccccgcag
     1321 ccagaggtca tatggcggca tcgactgaac cacgtcgtcg tgaaggagag cgacgaggga
     1381 gtcgaggtcg tatcgctcga aggcgtcgac gtaccggctg agcagggctt gctgctcttc
     1441 gtccaggggc cggtacagat ctgatctggc cggctctatc gctgagagtg tgaccctcgc
     1501 acgctgaagt gcgctgttga cagaggcaac cgtggtctcc agcaactcgg cgacctcgtc
     1561 tgccttccag cgcaggactt cgcgcaagat caagacggcc cgctgtcgcg ccggaagatg
     1621 ctgcagggcg gtgatgaagg cgagccgaac ggactccctg gcaatcgccg cctccgctgg
     1681 atcggggctc ggggcgagga tgatgctgtc gggggccggc tcgatccatg tgacctccgg
     1741 taacggtgcg ggaagcatcg tgtctgccgt cgaggccggc ccgaggtcca tggggcgagc
     1801 ccggcgttcc cgaccctcga gcatgtcgag gcagacattg gttgtgatgc ggtagagcca
     1861 ggttcgcagg gaggagcggc cctcgaatcg gtcgctggcc cgccatgccc tgatcaagct
     1921 gtcttgaacc gcatcctcgg cctcgaacga agagcccaac atccggtagg cgtaggcgtt
     1981 gagctcacgc cggtattgct cgagatcctc cagcgagggt gtgtcgcttc gaggctcggt
     2041 tgcagtcgat tcgctcatgt ggtccccagt atgggacatc tacatctcca gtcggaccgg
     2101 gaataggacc actgcccgat gaccggacat ctctgccgag caatcctgat cgtgattgcg
     2161 gtctgaatcc acggggtaac ccccacagct gtccactctc gcgcaatcga gaggagtaat
     2221 catgcatagc ccttccacca ccaatgagct ttcgatccgt gagctccgcg aggcgctcga
     2281 cggagaggtg atcacacccg acgaccattc atatgacgag gcacggcacg ttttctacgg
     2341 tatcgatcgc aggcctgcgg tgatcatccg gcccaccaat gccgacgagg tggcgtatgt
     2401 cgtctccgtc gctcgggatt ccggagcgga gctggcgatc cggagcggcg gtcacagcat
     2461 cgccgggtat ggaagctcgg acggcggtat cacgctcgac ctctcagaga tgaagtcagt
     2521 ccgggtcgat tcagagcacc gcaccgcctg ggcccagacc gggttgacgg ccggcgagct
     2581 caccacggag cttggaaagt atggcctcgc cgtcgggttc ggcgataccg gctccgtggg
     2641 gataggcggg atcacgctcg ggggcggagt gggattcctc tcccggaagt ttggcctgac
     2701 catcgactcc ctgctcgccg ccgagctggt gacggccgac ggaaaggtgc tccaggtgga
     2761 taccgagagc caccccgatc tcttctgggc gatcagaggt ggcagtggca acttcggagt
     2821 ggcgacgagc ttccagtacc gcctccacga agtcgatcag atggttgggg gaatgctgat
     2881 cctcccggcc acaccggaga cgattgccgg gttcgtggag cttgccgact cggcccccga
     2941 cgagctgtcg actatcgcca acgtgatgaa agcccctccg atgccgttcc tccctgagga
     3001 gttgcacggc caggtgatcc tgttcaccct cctcgtgtac gcgggggacc cagagaccgg
     3061 cgaagaggtc atcgccccgt tccgggagct ggcaagaccg ctgtcagaca tggtggagcc
     3121 gggcccttat gcggccatct atgaggacga agaggagggc ccaccccctg gagttttcgc
     3181 cgttcgcaat ctgttcgtcg acaagatcga ccgagcggcg gcagagacca tcatcgagca
     3241 cgtcaccgcg tccacagcgg cgatggccgt agctcagatc agggttctgg gcggagccat
     3301 cgcgcaggtt ccggaagaga ccacggccta cgcccaccgc cgggaaccga tcatgctgaa
     3361 catcgcgtcg atgttcgagc agccggacga agccgccact catcaagtgt gggtggaccg
     3421 cctcgccacc gccctcggcg acaagggcac ggcctatgtc aatttcatcg gcgacgaggg
     3481 cgagagcagg gtcagacagg cctatccggg ccaaacctgg gagcgtctcc ggcaggtcaa
     3541 gcagcgatac gacccgacga atctcttccg ggtcaatcaa aacatccctc cggccggcga
     3601 ctgactgagg cggagccagc cggggatcat ccaggcactg cggatcaggt gatgagacga
     3661 catcaaacca attggcggcc cacctgatac gaccggcaca accagtcctg gacctcttca
     3721 tcgaggtcgt cggtggaagt cacacgaacg tggtgtaccc aatcggtgcg gccgtagagg
     3781 accaccttgc ggagccgtgg gc
//
LOCUS       DASTXO010000074        10931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_446030, whole genome shotgun sequence.
ACCESSION   DASTXO010000074 DASTXO010000000
VERSION     DASTXO010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 10931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10931
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446030"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VFU96_01410"
     CDS             <1..545
                     /locus_tag="VFU96_01410"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEU5111941.1"
                     /translation="IPILIFALMLPFFGQGETTQVLGLTLSVEGLGGAWTILAKATLG
                     VAASIVVSATTTIPDMLRGLDRIRVPRVLTAIAGFMVRYLDIVAGEFSRRRVAMAARG
                     YEPRWLWQNGPLAQSAGSLFIRSFERGERVHQAMVARGFVGRMPVYGDRPATTREWWI
                     GLSVPLAAVLLAVLSVTVLR"
     gene            542..1291
                     /locus_tag="VFU96_01415"
     CDS             542..1291
                     /locus_tag="VFU96_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016815288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5111942.1"
                     /translation="MSEPSLEVIDLSFTYPDGRQALRNVDLHVHDGERVALLGPNGAG
                     KTTLLLHLNGILRPGSGEVRVAGLVVSDDSALEVRRRVGLVFQDPDDQLFMPSVGQDV
                     AFGPANFGVDGEDLEARVAEALGAISMLEARDRAPHHLSYGERRRVAIGTVLAMRPEI
                     LVLDEPTSNLDPASRRELIDVLRRLPITQLVVTHDLPFALELCPRAIVMDEGQIVAGD
                     NTVEILTDELLMKTHRLELPYRFDPREQAPT"
     gene            1312..2823
                     /locus_tag="VFU96_01420"
     CDS             1312..2823
                     /locus_tag="VFU96_01420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEU5111943.1"
                     /translation="MTIAWPARLNIDDHLLRQRLKEGNGGRVALRLDDRDVTYAEVDR
                     SASGYASLLADNGVGPGDRVLLVMPDGLEYVAALFGVLRSGAVGLLLNPGLTPSDLSA
                     IAGLARATGAIVHRGHLRSYEAALTSAGIPLPPGVVVDDDLGLPDVDVPTYDTAALDP
                     ALWIFSGGTTGVPKAVVQTHRSFANTTELYGKGTLGLTAEDITIAVPKLYFGYAMGSN
                     LFFPFSVGASAVLFQEQSTPEGLFDRIERHRATVLVNVPSAINQMLAHSDAADRDLSS
                     LRVATSAGEALPDTLYHQWTGRFGVELLDGLGTAEMWHIFVSNRIGDVRPGTLGRAVP
                     GFDIKVCDDDGEEVPAGEVGRLWVKGDSLGLGYFENPEATAEAFRGEWFAGGDLVSID
                     GGGYVTHRGRVDDAIKVKGRWFRPQEVESILLDHPAVNECAVVAITDAAGLAKPVAFV
                     VATDVTEGELTDWALAHLEAYKHPRRIHFVDRLPQTHLGKVDRAALKSLAADG"
     gene            2900..4129
                     /gene="dinB"
                     /locus_tag="VFU96_01425"
     CDS             2900..4129
                     /gene="dinB"
                     /locus_tag="VFU96_01425"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU5111944.1"
                     /translation="MGEPTILHADLDAFFASVEQLLDPSLKGKPVLVGGGIVLAASYE
                     ARACGVRSPMPTRDAKRLCPEAIVVNGHFERYLDLSAQVMEVLEDASPVIEQISIDEA
                     FIDVRGTTHLLGSPSAIARQIRSRVATEVGLPISVGVASTKFLAKIASARAKPNGLVV
                     VAPGTELDFLHPLPVEAMWGVGRVTAARLHRFGIFTIGDLAALPRGSLSSSLGPWASG
                     SLHSLSWNRDPRTVRGGRRAGSVGSQQALGRGLTDPEELSAVVLGLADRIGRRARKKE
                     RSGSTLTLRVRFPGPRLVSRSHTLPSPTSSTAALAGLGGVLLQRAIEGEEEPVTLVGM
                     SLSNLTTDDYLQLELEVDSGDVLRAGSGLDLKRRAIDASVDGIRERFGRELLKLGSAP
                     DRGVTPEEFRKLAEHSD"
     gene            complement(4121..4687)
                     /locus_tag="VFU96_01430"
     CDS             complement(4121..4687)
                     /locus_tag="VFU96_01430"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU5111945.1"
                     /translation="MTWLAEKWLKWRGWEFIGEMPDLDKFVLIGAPHTSNWDFIVYLG
                     AIRHWGISPRFVGKHTLFRWPFGYFFRRFGGIPVDRDRAGGMVGQVAEEFERSSRMIL
                     VVAPEGTRKAAPYWKSGFARIAAAARVPIVPVYIDYPARRLVLGDPISYSGDEKELMD
                     RLRVFFEPGVGKDRQGKGPVRLKEERSV"
     gene            complement(4713..6326)
                     /locus_tag="VFU96_01435"
     CDS             complement(4713..6326)
                     /locus_tag="VFU96_01435"
                     /inference="COORDINATES: protein motif:HMM:NF039189.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HEU5111946.1"
                     /translation="MVVAGLVFLAIVGGVIALVVRAVRGRDKQDEDGGLDLIPYLLLA
                     LFVVAAGFSLAALARASLTPDRLIGQPTGELAGALAGLVVAAPIAYFLGRRQARRRKS
                     FPQTPGWPVYLAIIELVFLIAFLAAVSQIAGFLGGEGGRADWTDLVIYGGIVVFHWWA
                     ERRERPAGDIGELPRLVGSGVALVAMTIGLIGTLEWLFSIAFDALSGLEDIPEPAVPI
                     ALAVTGAGIWGWRWLPTWDEEPTVLRNFYLGFVSAFSLTMAIGAGVGMIATLLTFALG
                     EGGPARDHFDDVPLALAFFIAGAGLWYHHSHRLGLGRTGARRGYEYAMAAVGLGALVG
                     STTALVDAAFTPMLAGTNRGSTLIATACAVIASGWVWLSFWRKAQAAPRPEEARAVSR
                     RIYLIGMAIVLGLTAAGSLIAVLVVVFRTVLGEIEASAGSLRVPVTLTLTAGLATWHL
                     FDQIRADGSGRERTVSRPFAVTVVCSHPGTLGTRFPEEATVRILYRGDDAGVVDDEMA
                     ADIVAAVDGRSSLVWVDERGFRLAPARES"
     gene            complement(6332..6775)
                     /locus_tag="VFU96_01440"
     CDS             complement(6332..6775)
                     /locus_tag="VFU96_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111947.1"
                     /translation="MEEDRRLPGWVLPLVGVLAVVALVVVGLNRSPDQFDPASPEGTV
                     QSYIAALVAGDFETAATFWAEDDCLPASIEPTEGAPDISATLVSVDGDETEATVVIGI
                     TDNTSDPLNGVAEYQEWFTLVRGDTGWKIRQPSWPYYDQICEASA"
     gene            complement(6825..7166)
                     /locus_tag="VFU96_01445"
     CDS             complement(6825..7166)
                     /locus_tag="VFU96_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU5111948.1"
                     /translation="MPCRQGAGIGLNLHQMIENVPAQDWESWIESNGGTVLDIREPFE
                     WEQGTLPDSTLIPMGEIPERCGELDIEQAFLVVCRSGARSQQVAAFLTMNGFTRVANM
                     AGGMKALGLQD"
     gene            7206..7808
                     /gene="purN"
                     /locus_tag="VFU96_01450"
     CDS             7206..7808
                     /gene="purN"
                     /locus_tag="VFU96_01450"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEU5111949.1"
                     /translation="MEVIINIAVLASGSGTNLQALIDAPNLGGDIELVLSDRSDAIAL
                     DRARAAGIPTTVVSWGDYGSRTEFSTAVMEQMERADVEIVVLAGFMRVLGADLVGTFK
                     NQILNIHPSLLPAFPGARAVDQALEHGVKMTGVTVHFVDEEVDHGPIIAQRAVPVLPD
                     DDRETLHARIQVEEHDLYPKVVAALARGDIAVEGRKVRWF"
     gene            7799..9307
                     /gene="purH"
                     /locus_tag="VFU96_01455"
     CDS             7799..9307
                     /gene="purH"
                     /locus_tag="VFU96_01455"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018581092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU5111950.1"
                     /translation="MVLSRVVVKRALISVYDKTGLVSFAERLAGAGVEIVSSGGTAVA
                     LTVAGIEVNTVAEVTGAPEMLGGRVKTLHPSIHGAILARTDDEQDLADLQANGIEPFQ
                     LVVVSLYPFRETLATPGVTEPEIIEKIDIGGPTMIRAAAKNHRFVGVVTSAGQYDMVA
                     EAVERGGLDDELRRALATEAFFHTAAYDAAIVGWIGEDRVLPLRHVTDLRYGENPHQP
                     GSIYVEDGSGPWWAGAEQLQGKEMSFNNYVDAEAAWRLVSDKEGCVAIVKHTNACGAA
                     YGDEVAEAFERAWACDPLSAFGGVIGINGSLDATTAKSIAEKFVEVVVCTGVDDEARV
                     VLGPKENLRVLEARPPGQGDPDLRRVESGVLIQERDTTIGEQWEVVSERQLTDVERDE
                     LEFAWIVAMHTKSNAIVVVKDRAAVGVGAGDQSRIGAAERALSRAGERSVGAVAASDA
                     FFPFRDGLDVLAQAGVTAVVEPGGSRNDAELIAAANEHGIALVFTGRRHFKH"
     gene            9307..10155
                     /gene="folD"
                     /locus_tag="VFU96_01460"
     CDS             9307..10155
                     /gene="folD"
                     /locus_tag="VFU96_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009141609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEU5111951.1"
                     /translation="MSALVLDGKQSAAKVKTEVSAAVAKLDYLPGLATVLVGDDPASH
                     TYVRGKRSDAEEVGIQSFHHELPADIPQDDLGAVIAELNADDAVDGILVQLPLPEGLD
                     SERIVSSIDPAKDVDGLHPYNLGLLVLGRPGLRPCTPSGIMRMLEDHGVAVAGKTALV
                     IGRSFLVGRPLALMMAAKGVDATVTMAHSRTPRLAEVSRAADILVAAVGVPRMVTAEY
                     VKPGAAVIDVGISRVEDGLAGDVDFESVREVAGSITPVPGGVGPMTRAMLLVNTLAAA
                     RGRRES"
     gene            10188..10631
                     /locus_tag="VFU96_01465"
     CDS             10188..10631
                     /locus_tag="VFU96_01465"
                     /inference="COORDINATES: protein motif:HMM:NF025215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HEU5111952.1"
                     /translation="MRSSLRTLTGLVALALLLTACFGSGNVFELAVGDCFDDGDLVLG
                     ELEEIGDVPLVECSEPHDNEVYAVVDVDGEEFPGEQAVQDQADEACLSTFDAFVGLDY
                     ESSTLDFGWLVPTADSWDMGDRVIACFVYRMDLEKVTGTLEGASI"
     gene            10704..>10931
                     /locus_tag="VFU96_01470"
     CDS             10704..>10931
                     /locus_tag="VFU96_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEU5111953.1"
                     /translation="MATSITMGPDGLVVPNDPIIPFIEGDGIGPDIWAASVRVFDAAV
                     KGAYGDERAVAWKEVLAGEKAFTETGSWLPDE"
BASE COUNT         1880 a         3565 c         3609 g         1877 t
ORIGIN      
        1 agatcccgat cctgatcttc gccctcatgc tccctttctt cgggcaaggg gagaccaccc
       61 aggtcctcgg gctcactctt tcggtcgagg gtctgggggg ggcttggacg atcctggcca
      121 aggccactct cggggttgcg gcgtcgatcg tggtatctgc gacaacgact atccccgaca
      181 tgctccgcgg gctggaccgg atccgggtgc ctcgggttct caccgccatc gccggtttca
      241 tggtgcgcta cctggacatc gtcgccggcg aattctcccg gcgaagggtg gccatggccg
      301 cccggggcta cgagccccgc tggttgtggc agaacgggcc gctggcccag tccgccggat
      361 cgctcttcat ccgatcgttc gagcggggcg aacgggttca ccaggcgatg gtggcgaggg
      421 gattcgtagg aaggatgccg gtctatggcg atcgtccggc gaccacccgg gaatggtgga
      481 tcggtctctc ggtgcccctc gcggccgttc tcctggccgt cctctcggtg acggtgctgc
      541 gatgagcgag ccgtctctcg aggtcatcga tctgagcttc acctatcccg acggtcgtca
      601 agcgctccgc aatgtcgatc tgcacgtgca cgacggggag cgggtggccc tcctcgggcc
      661 caatggggca ggcaagacca cccttctcct ccacctcaac gggattttga ggccgggctc
      721 cggagaggtg cgtgtggccg gtctggttgt ctccgacgat tcggccctgg aagtcaggcg
      781 aagggtcggc ttggtgttcc aggaccccga cgaccagctc ttcatgccga gcgtggggca
      841 ggacgtcgcc tttggcccgg ccaacttcgg cgtcgatggc gaggacctcg aagcacgcgt
      901 cgccgaggcc cttggcgcca tctccatgct ggaggcgcgg gaccgggccc cgcatcatct
      961 cagctatggg gagcggcgac gagtggccat cggcaccgtt ctggcgatgc gtcccgagat
     1021 actggtcctc gacgagccga cctcgaacct cgacccagct tcgcgacggg agctgatcga
     1081 cgtgctccgc cgcctgccga tcactcagct ggtcgttacc cacgacctcc cattcgccct
     1141 ggagttgtgc cctcgcgcca tcgtcatgga cgaagggcag atcgtggcgg gggacaacac
     1201 ggtcgagatt ctcaccgacg agttgttgat gaagacacat cgtctggagc tgccatatcg
     1261 gttcgacccg agggagcagg cgcctacctg accgcgcggt aacttccgct cgtgaccatc
     1321 gcctggcccg cccggctcaa catcgacgat cacctgttac ggcagcgtct gaaggagggg
     1381 aatggcgggc gggtggcgct ccgtctcgac gatcgggacg tcacctacgc cgaagtcgac
     1441 cggtctgcat cggggtatgc gtcattgctt gcagacaacg gcgtagggcc cggtgaccgg
     1501 gtgctccttg tcatgcccga tgggctcgaa tacgttgctg ccctattcgg agtgctccga
     1561 agcggagcgg tcggcctcct cctcaaccca ggactcaccc cgagcgatct gtccgcgatc
     1621 gccggtctgg cgcgggcgac gggagccatc gtccatcgag gccatcttcg ctcatacgag
     1681 gcggcgctca cctcagccgg gatcccgttg ccgccgggcg tggtggtcga cgatgacctg
     1741 ggcctgcccg atgtcgatgt ccccacgtac gacaccgccg ccctcgatcc ggcgttgtgg
     1801 atcttctccg ggggcacgac cggtgtcccc aaggcggtgg tacagactca caggtctttc
     1861 gccaacacca ccgagttgta cgggaaaggc actctggggt tgaccgcgga ggacataacc
     1921 attgccgtac ccaagctcta tttcggctac gccatggggt cgaatctctt cttccccttc
     1981 tccgtcggtg cctcagcagt gctcttccag gagcaatcca cccccgaagg tctattcgac
     2041 cggatcgaga gacatcgagc gaccgttctg gtcaatgtgc cttcggccat caatcagatg
     2101 cttgctcact ccgacgctgc ggaccgggac ttgtcatcgc tccgggtggc tacttccgcc
     2161 ggcgaggcgc tacccgacac gctgtaccac cagtggacgg ggcggttcgg ggtcgagttg
     2221 ctcgatgggc tcgggaccgc ggagatgtgg cacatcttcg tctcgaaccg gatcggggac
     2281 gtgcgacccg gcaccctggg gagggctgtc cccggcttcg atatcaaggt gtgtgacgat
     2341 gacggggagg aggtgcccgc cggcgaggtc gggcgtctgt gggtgaaggg tgactcgctc
     2401 ggtctgggtt atttcgagaa ccccgaggcg acggccgagg ccttccgtgg tgagtggttc
     2461 gcgggtgggg acctcgtctc gatcgacggt ggcgggtatg tcactcaccg gggccgtgtc
     2521 gacgacgcca tcaaggtcaa aggaaggtgg ttccgtcccc aggaggtcga atcgatcctc
     2581 ctcgatcacc cagccgtcaa tgagtgcgcg gtggtggcga taaccgacgc ggccggcctg
     2641 gccaaaccgg tcgccttcgt ggttgcaacc gacgtcaccg agggtgagtt gaccgactgg
     2701 gccctcgccc atctcgaggc ctacaagcac ccccgtcgga tccatttcgt cgaccgtctc
     2761 ccccagacgc atctgggcaa ggtcgaccgg gccgcgctta agagtctcgc cgcggatggc
     2821 tgaaccgcgg caacgggcgg aaacggatgg ggctatcccc cgggattagc tgtcctagcc
     2881 accgggcata ctgagggaca tgggagagcc gaccattctc cacgcagatc tggacgcgtt
     2941 cttcgcttcg gtcgaacagc tcctcgaccc ttcgttgaaa gggaaaccgg tcctggtcgg
     3001 gggtgggatc gtgctggccg cgtcctatga ggcgcgggcc tgcggggtcc gatcgccgat
     3061 gccgacccgc gacgccaagc ggctctgccc cgaggccatc gtcgtcaacg ggcacttcga
     3121 gagatatctc gacctttcgg cccaggtcat ggaggtcctc gaggacgcat cccccgtcat
     3181 cgagcagatc tctatcgacg aagccttcat cgacgtgagg gggaccacgc atctcctcgg
     3241 gtcaccatcg gcgatagctc gtcagatccg atcccgtgtc gccaccgagg tcggcctgcc
     3301 catctcggtc ggcgtcgcct ccaccaagtt cctggccaag atcgcttccg cccgcgccaa
     3361 accgaacggt ctcgtggtgg tggcgccagg caccgagctc gattttctcc acccgctacc
     3421 ggtcgaggcg atgtggggag tggggcgggt cacggccgcc cgcctccatc gcttcgggat
     3481 cttcaccatc ggagacctgg cggcgttgcc ccgcggctcc ctctcctctt cgcttggccc
     3541 gtgggcctcg ggctcgctcc attcactgtc gtggaaccgg gatccgagga cagttcgcgg
     3601 ggggcgaagg gccggaagtg tcggtagcca acaggcgctt ggtcgcggtc tgaccgatcc
     3661 agaggagctg tcggcggtgg tgctcgggct cgccgatcgg atcgggaggc gagcccggaa
     3721 gaaggagcgg tcgggatcga cgctgactct ccgggtcagg ttccctggcc cacgcctggt
     3781 gtcccggagt cacacccttc cctcaccgac ttcgtcgact gccgcgctcg ccggtcttgg
     3841 cggcgtcctc ctccagcggg caatcgaggg ggaggaggag ccggtgactc tggtcggcat
     3901 gtcgctgtcg aatctgacca ccgacgacta cctccaactc gagctggagg tcgactcggg
     3961 agacgtcctc cgcgccggga gcggcctcga cctcaagcgt cgggccatcg acgcatccgt
     4021 cgacgggata agagagcgtt tcgggaggga gctgctcaag ctggggtcgg ctcccgatcg
     4081 gggtgtcacc cccgaggagt tcagaaagct ggccgagcac tcagactgac cgctcctcct
     4141 tgagccgaac cgggcctttt ccttgccggt ccttgccgac accgggctcg aagaagaccc
     4201 gcagtcgatc catcaattcc ttctcgtcgc cggagtagga gatcgggtcg ccaagcacca
     4261 gcctccgtgc cgggtaatcg atgtagaccg ggacgatcgg gacccgggct gcggctgcga
     4321 tacgagcgaa acccgacttc caatacggcg ccgccttgcg agtaccttcg ggcgcgacga
     4381 cgaggatcat ccgggacgac cgttcgaatt cctccgccac ctgcccgacc atgccaccgg
     4441 cccggtcacg atcaaccggg atgccgccga acctcctgaa gaagtaaccg aacggccaac
     4501 ggaacaaggt gtgcttgccg acgaaccgcg gggagatccc ccagtgcctg atcgcaccca
     4561 gatagacgat gaaatcccag ttgctggtgt gaggagcccc gatgaggacg aacttgtcca
     4621 agtcgggcat ctcgccgatg aactcccagc cccgccattt gagccatttc tcggcgagcc
     4681 atgtcatggc aatcgcgcgg gctcgggggc gatcaagact ccctcgccgg ggccaacctg
     4741 aagcctctct cgtccaccca gaccagagag gagcgcccat cgacggcggc gacgatatcc
     4801 gcggccatct cgtcgtcgac cacgccggcg tcgtctcccc gatagaggat cctgacggtc
     4861 gcctcctcgg ggaaccgggt gccgagagtg cccgggtggg agcagaccac ggtcacggcg
     4921 aacggtctcg acacggttcg ctcccgtccg gacccatcgg cgcggatctg gtcgaagaga
     4981 tgccatgtgg ccagcccggc ggtcagcgtc agagtgaccg ggacgcggag gcttccggcg
     5041 cttgcctcga tttctccgag cacggtgcgg aacaccacca ccaggactgc gatcaaggat
     5101 ccggccgcgg tgaggccaag gacgatggcc atccctatca ggtagatgcg acgggacacg
     5161 gcgcgggcct cctcagggcg cggcgccgcc tgtgcctttc gccagaagga caaccagacc
     5221 caaccggacg caatcacggc gcaagcagtc gctatcagtg tcgagcccct gttcgtcccg
     5281 gccagcattg gagtgaatgc ggcatccacc aaggcggtag tcgagccgac gagggcaccg
     5341 agacctaccg ccgccatcgc atactcatac ccacgccggg cgccggtgcg ccccagaccg
     5401 aggcggtggc tgtggtgata ccagagaccc gcgccagcga tgaagaaggc cagggcaaga
     5461 gggacgtcat cgaaatggtc cctcgccggc cccccctcgc ccaacgcgaa tgtgagcaga
     5521 gtggcgatca tgccgacccc ggctccgatg gccatggtca gggagaaggc gctcacgaag
     5581 ccgagataga aattgcgcag gacggtcggc tcttcgtccc aggtcgggag ccaccgccag
     5641 ccccatattc ccgctccggt cacggcgagt gcgatgggga ccgcaggctc cggaatgtcc
     5701 tccagccctg agagcgcgtc gaaggcgatg gagaagagcc actcgagcgt gccgatgaga
     5761 ccgatggtca tggcgaccaa tgcgaccccg gacccgacca ggcggggcaa ttcgccgatg
     5821 tcgcccgcag ggcgctcacg ccgctccgcc caccaatgaa atacgacgat ccctccgtag
     5881 atcaccagat cggtccagtc cgccctgccg ccctcgccgc ccaggaaacc ggcgatctgg
     5941 gagactgcgg ccaggaaggc gatcaggaat acgagctcga tgatcgccag gtagacgggc
     6001 catccggggg tctgggggaa tgactttcgg cgtcgcgcct gccgacgccc gaggaagtag
     6061 gcaatggggg cggccacgac caggccggcc aacgctccgg cgagctctcc ggtcggttgc
     6121 ccgatgaggc gatccggagt gaggctggct cgggcgagag ccgcgaggga gaacccggct
     6181 gccaccacga acagggcgag caggaggtag gggatcaggt cgagaccacc gtcctcgtcc
     6241 tgtttgtcac gacctcggac cgcccgcacc accagggcta tgacaccccc gacgatcgcc
     6301 aggaagacga gaccggccac caccacggtg ctcaagccga cgcctcgcag atctggtcgt
     6361 agtacggcca cgatggctgg cggatcttcc agccggtgtc gccccgcacc agggtgaacc
     6421 actcctgata ctcggcgacg ccgttcaggg ggtcagatgt gttgtccgtg atcccgatga
     6481 cgacggttgc ctccgtctcg tcgccgtcga cgctgaccag agtcgccgag atgtcgggcg
     6541 ctccttcggt gggctcgatc gaagcgggga gacagtcgtc ttccgcccag aaggtggccg
     6601 cggtctcgaa atcccctgcc accaaggcag cgatgtacga ctgaacagtt ccctcgggcg
     6661 atgcggggtc gaactgatcc gggctgcgat tcagaccaac aaccaccagg gcaaccactg
     6721 cgaggacccc gaccaggggc agcacccacc ccggcaacct gcggtcctct tccatacttg
     6781 gtgattctcg caggtcccct ttcacaaatc gggaccgcgg gccctcagtc ctgaagaccc
     6841 agcgccttca tcccgccggc catgttggcc actcgggtga agccgttcat cgtgagaaag
     6901 gccgccacct gctggctccg cgccccagag cggcagacca cgagaaacgc ctgctcgatg
     6961 tcgagctcgc cgcaacgctc aggtatctca cccatcggga tcagcgtgga gtcggggagg
     7021 gtgccctgct cccattcgaa cggttcgcgg atatcgagga ccgttccccc gttggattcg
     7081 atccagctct cccagtcctg tgccggcacg ttttcgatca tctgatggag attcaagccg
     7141 atccccgcgc cttgtcggca cggcatccgt cacctctaca ctcccgccgc ggtcgaccgg
     7201 gtcgagtgga ggtcatcatc aacatcgcag tcctcgcctc tggctccggc accaatctcc
     7261 aggcgttgat cgatgcgccg aacctgggag gggacatcga actggtcttg tccgatcgga
     7321 gcgacgcgat cgctctggac cgggcgcgag ccgcaggcat cccaaccacc gtcgtctcct
     7381 ggggtgacta cggctcccga accgagttct ccaccgccgt gatggagcag atggagcggg
     7441 cggatgtgga gatcgtcgtg ctcgcaggat tcatgcgagt cctcggcgcg gacctggtcg
     7501 gcacattcaa gaaccagatc ctcaacatcc atccttcgct cctgcccgcc tttcccggtg
     7561 cccgtgccgt tgaccaggcc ctggaacatg gcgtgaagat gaccggggtt accgttcatt
     7621 tcgtggacga agaggtcgac cacgggccga tcatcgccca gcgagccgtg ccggtgctgc
     7681 ctgatgacga ccgggaaacc ctccatgccc gcatccaggt ggaggagcac gacctatacc
     7741 ccaaggtggt ggccgcgttg gcccggggtg atatcgcggt cgagggccga aaggtgagat
     7801 ggttttgagc cgtgtcgtgg tgaaacgagc gctgatcagc gtctacgaca agaccgggct
     7861 ggtctcattc gccgagcgtt tggccggggc gggcgtggag atcgtctctt cgggcggcac
     7921 cgccgtggcg ttgaccgtcg ccggtatcga ggtcaacacc gtcgccgagg tcaccggggc
     7981 gcccgagatg ctcggaggaa gggtcaagac tctccaccct tcgatacatg gcgcgatcct
     8041 ggcccggacc gacgacgagc aggatctcgc cgatctccaa gccaacggga tcgaaccttt
     8101 ccagttggtc gtagtcagcc tctacccgtt ccgggagaca ctcgccacgc cgggcgtcac
     8161 cgagcccgag atcatcgaga agatcgacat cggcgggccg acgatgattc gggccgcggc
     8221 taagaatcat cgtttcgtgg gagtggtcac ctcggccggc cagtacgaca tggtggccga
     8281 ggccgtcgag cgtggtggcc tcgacgacga gctgaggcgg gccctcgcta ccgaggcctt
     8341 cttccacacg gctgcatacg acgccgcaat cgtgggctgg atcggcgagg atcgggtgct
     8401 tcctctgcgc cacgtcaccg acctgcgtta cggggagaac ccacatcagc caggctcgat
     8461 ctatgtcgag gacggctcgg gtccttggtg ggccggcgcc gagcaactcc agggaaaaga
     8521 gatgtcgttc aacaactacg tcgatgccga ggcggcatgg cggctggtct cggacaagga
     8581 aggctgcgtg gccatcgtca aacacaccaa tgcctgcggt gctgcctacg gagatgaggt
     8641 ggcggaggcc ttcgagcgag cctgggcgtg tgaccctctg tcggccttcg gcggtgtgat
     8701 cggcatcaac ggcagtctcg acgcgaccac cgcgaagtcc atcgcagaga aattcgtcga
     8761 ggtcgtggta tgcaccggag tcgatgacga ggcccgcgtt gtgctcggac ccaaggaaaa
     8821 cctgcgtgtc ctcgaagcac ggccccccgg gcagggtgat ccagacttgc gccgggtcga
     8881 atccggcgtc ctgatccagg agcgcgacac gacgatcgga gagcagtggg aagtcgtctc
     8941 cgaacgtcag ctcacggatg tcgagcggga cgagctcgag ttcgcctgga tcgttgcgat
     9001 gcacacaaag tcgaacgcga tcgtcgtcgt caaggatcgg gcggcggtcg gcgtcggggc
     9061 gggcgaccag tcgcggatcg gggcggcgga aagggcgttg tcccgggcag gggagcggtc
     9121 ggtcggggcc gtggccgcat cggacgcttt cttcccgttt cgggacggtc tcgacgtcct
     9181 ggcccaggcc ggcgtcaccg ccgtcgtgga gccgggcggt tcgcgaaacg acgccgagct
     9241 gatcgcagcg gcaaacgagc acgggatcgc cctggttttc accgggcgca ggcatttcaa
     9301 gcactgatga gcgccctggt actcgacggc aagcaatctg ccgcaaaggt gaagaccgaa
     9361 gtctcggcgg cagtcgccaa actcgattac ctgcctgggc tcgccacggt gttggtgggg
     9421 gacgatccag cctcacacac ctacgtccgt ggcaagcgca gcgacgccga agaggtcggc
     9481 atccaatcgt ttcatcacga gctaccggcc gacatccctc aggacgacct gggggcggtc
     9541 atcgccgagc tcaacgcgga tgacgcggtg gatgggatcc tggttcagct cccgctcccc
     9601 gaaggtctcg actcggagcg aatcgtgtcg tccatagacc ctgccaagga cgtcgacggg
     9661 ctgcacccgt acaacctcgg gcttctggtc ctgggccggc ccggtctcag gccatgcacc
     9721 ccgagcggga tcatgcggat gctcgaggat cacggggtag ctgtcgccgg gaaaacagcg
     9781 ctcgtcatcg ggcgttcgtt tctcgtgggg cgccccctgg ccctgatgat ggcggcgaag
     9841 ggagtcgacg ccacggtgac gatggctcat tccaggaccc ctcggctggc cgaggtatcg
     9901 cgggccgccg acatcctggt ggccgcggtc ggggtcccgc gcatggtgac cgccgagtac
     9961 gtgaaacccg gcgctgcggt gatcgacgtc gggatcagcc gggtcgagga cgggttggcc
    10021 ggggacgtcg acttcgaatc ggttcgggaa gtggcgggct cgatcacgcc ggttcccggc
    10081 ggtgtgggcc cgatgacgag ggcgatgctc ctggtcaaca ccttggcagc ggcccggggg
    10141 cgacgagagt cataaagaat cgcgtccctt tggtcgaaat tgaccgaatg cgcagctcac
    10201 tacgaacact gaccggtctg gttgccctgg cgctgctgct gaccgcctgc ttcgggtccg
    10261 gcaacgtgtt cgaactcgcc gtcggagact gcttcgacga cggtgatctg gttctcggcg
    10321 agctggaaga gatcggtgac gtccccctgg tcgagtgctc cgagccgcac gacaacgagg
    10381 tgtatgccgt cgtcgacgtc gatggggagg agttccccgg cgagcaagcc gtccaggacc
    10441 aggccgacga agcctgcctc tccaccttcg acgccttcgt cggcctcgac tacgagagct
    10501 ccactctcga cttcggatgg ctggtgccga ccgccgactc ctgggatatg ggcgaccggg
    10561 tgattgcctg tttcgtctac cgcatggacc tcgagaaggt gaccgggact ctcgaggggg
    10621 cgagcatctg aaaccggggc ctggaatccg ggcaacggcg cgcagctaac ctcgacgttt
    10681 cctacgaatt cccaggagtc tcaatggcaa cgtccatcac catgggcccg gacggtctcg
    10741 tcgtgcccaa tgatccgatc atccccttca tcgagggtga cgggataggc cccgacatct
    10801 gggcggcctc ggtccgagtg ttcgacgccg cggtgaaggg cgcctacggc gacgaacgcg
    10861 ccgtggcctg gaaggaagtc ctggccggcg agaaggcctt cacggaaacc ggctcctggc
    10921 tccccgatga g
//
LOCUS       DASTXO010000075         3814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_667569, whole genome shotgun sequence.
ACCESSION   DASTXO010000075 DASTXO010000000
VERSION     DASTXO010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667569"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..1273
                     /locus_tag="VFU96_01475"
     CDS             266..1273
                     /locus_tag="VFU96_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU5111954.1"
                     /translation="MARTLDDLAELSGVSRATVSRVINGGPVSEETRRRVLEVIEGTN
                     YRPNLAARSLASGRTGVVGVVMHVEPPVLFEDSYFVALLHGVSDVLADRASGMMLWLG
                     NRSKEETLDRILGTGLMDGVIVTADNLDDPLVDGLLASDLPTVLIGHRRSDLTASYVD
                     IDNIGAADTVTTHLIDIGRTRIGHITGRLGSVAGEDRLVGYRRAMKRAGLDADRFVIE
                     GDFTRPSAIEVAAKLLELDVDAIFCANDATASGALETIKAAGLDVPKDVALAGFDDLP
                     VASELSPPLTTMRQGVHAQGIESASALLSLLESPEGGPRRVLLPTELVIRQSTVGGLS
                     G"
     gene            1367..2830
                     /locus_tag="VFU96_01480"
     CDS             1367..2830
                     /locus_tag="VFU96_01480"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU5111955.1"
                     /translation="MKKTKSTALVVVLILILGACGDSGGTGGSSTTAASSEESTTTAA
                     AAATTAAPSGDKVEIRWFVGLGAGDQPEQKAAQEAVVAAFNESQDEIELVLEIVDYDV
                     AYTTLANQIATGDAPDIIGPVGRDGSNGFAGLYLDIEPLIASTGVETSQWATAAVDNQ
                     READGTLVGLPFGSFPSAIYFNRDLFAEAGLPEPPQEYGPDGVTVYGEGTEYEGTWDW
                     AKVQELAEILTVDGNGVDATDPAFDRSTATQWGFHWQFTTGLFQNGSFWGAGYPMADD
                     GTADIPAIWEDEWKWFHANVWERGISPDQTVLDAGDNLFQGGNIAMAATHLWYTCCIQ
                     SDTIAGDFWDYGVMPSNNGVVTANLHADTFRILDSTEHPDEAFTAMYYLLTEAALDLL
                     TAYGAAPADPALMDAYLAAQDERYPQGVNWQTILDGANYADNPSHETFLPGWSEYKIR
                     LKELETGLLSNPDLDLDAEIAQVESDLSAIFAENAEG"
     gene            2848..3807
                     /locus_tag="VFU96_01485"
     CDS             2848..3807
                     /locus_tag="VFU96_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU5111956.1"
                     /translation="MTVIVNEGAAEHPAAPSRPARSSLARRRGRAGIWFVMPWILGFF
                     LWYAIPMAASLWFSFTDYDLVSDEPMQFVGLDNWVRMFNDADVHQSVLVTLKFGAIAL
                     PVAILFPMAIAYLLVAKALKGREGFRALFYLPSIIPFVAAVLIFNGVFNGQIGWVNKI
                     LAIFGIDGPDWFHDEFWIYPSLVFIGLWAVGNAILIFIAAMKGVPSDLYAAARIDGAG
                     EWGSFRHITIPFITPIIFYNLVITLIGIFGYFLVPFVLNNGSGAPNGATQFYALYFFR
                     TGFQFFDMGYAATLAWALFIVAVLTTGALFWSAKYWVHYEFEG"
BASE COUNT          698 a         1200 c         1205 g          711 t
ORIGIN      
        1 tgccgaggtc atccagaggt tcgccgccac cacccccacg gtgcaggccc ctgtcaagac
       61 cgtgaccctc gacgtcactc ccgaccagta ctacgacgat ccggcaaccc acgcccgacg
      121 tctctatcag gactggtagc cctcccgttc tgggagcgtt tccaacctgc ccgagagaaa
      181 tgcctcagtt attgcttgac agcgcctacc taggctctgt acgtttgaga gcgctctcag
      241 catttgaggg aggaggcccg ggagaatggc gaggacgctc gatgatctag ccgagctgag
      301 tggtgtctcc cgagccaccg tctcccgggt gatcaatggg gggccggtct cggaagagac
      361 caggcgacga gtcctcgagg tgatcgaggg caccaactat cgaccaaacc tcgccgcacg
      421 gtcccttgcg tcggggcgca ccggtgtggt gggtgtggtc atgcatgtcg agcctcctgt
      481 cctcttcgaa gactcctatt tcgtggccct gctacacggg gtgagcgacg tgctcgccga
      541 tcgggcgagc gggatgatgc tgtggctggg caaccggagc aaagaagaga cgctggaccg
      601 aatcctgggc acaggtctca tggacggcgt catcgtgact gcggacaacc tcgatgaccc
      661 gctcgtcgat ggcctgctcg cttcggacct gcccaccgtc ctcatcggcc accggcggtc
      721 tgacctgact gccagctatg tcgatatcga caacataggt gccgcggaca ccgtcacgac
      781 ccacctgatc gatatcgggc ggacccggat cggccacata accggacgtc tgggcagcgt
      841 ggccggcgaa gaccggctgg tgggttaccg gcgggcgatg aagcgcgccg gcctcgacgc
      901 cgatcgtttc gtaatagagg gcgacttcac ccgcccatcc gcaatcgagg tcgctgcgaa
      961 gctgctcgaa ctggacgtcg atgccatctt ttgcgcgaat gacgccactg cgagcggggc
     1021 cctggagacg atcaaggccg cgggtctgga tgtgcccaag gacgtggcgc tggccgggtt
     1081 cgacgatctc ccggtcgcat ccgagctcag ccctccactg accacgatgc gtcagggggt
     1141 gcacgcccag ggcatcgagt cagcctctgc cctcctcagc ctcctggaga gcccggaagg
     1201 tgggccgcgg cgagtgcttc ttccaaccga gttggtcatc agacaatcga cggttggagg
     1261 tctatcggga tagcccgaat gaaaggtcct gtcagtcccc ggtgcaggac cgcagcggcc
     1321 acgcggccgt tgaaataccc ggggcagaag gaggagaagg agaacaatga agaagaccaa
     1381 gagcaccgcc ctcgtggtgg tcctcatctt gatcctcggc gcgtgtggtg actccggtgg
     1441 caccggtggt tccagcacca cggcggcgag tagcgaggag tcgactacga cggccgctgc
     1501 tgcggccacg accgcggcac catcgggtga caaagtggag atccgatggt tcgtcgggct
     1561 cggtgccggt gaccagccgg agcagaaggc agcccaagag gcagtggtcg ccgcattcaa
     1621 cgagtcgcag gatgagatcg agctcgtgct cgagatcgtc gactacgacg tcgcgtacac
     1681 gaccctggcc aaccagatcg ctacgggcga cgcccctgac atcatcggtc cggtaggccg
     1741 agacggctcc aatgggttcg ccgggctcta cctggacatc gagcctttga tcgcgtcgac
     1801 cggggtcgag acctcacaat gggcgaccgc cgccgtggac aaccagcggg aagccgacgg
     1861 gaccttggtc ggtctcccgt tcgggtcctt cccgtcggcc atctatttca accgggacct
     1921 cttcgcggag gccggtctgc ccgagccgcc acaggagtac gggccggatg gcgtgactgt
     1981 ctacggtgag ggcacggaat acgagggaac ctgggactgg gccaaggtcc aggagctcgc
     2041 cgagatcctc accgttgacg gcaacggggt ggacgccacg gatcccgcct tcgaccgctc
     2101 gactgcgacc cagtggggct tccactggca gttcaccacc ggtctcttcc agaacgggtc
     2161 gttctggggc gccggttacc cgatggcgga tgacggcaca gccgacatcc cggcgatctg
     2221 ggaagacgag tggaagtggt tccacgccaa cgtgtgggag cgaggcatct caccagacca
     2281 gaccgtattg gatgcgggcg acaacctgtt ccagggcggg aacatagcga tggcggcgac
     2341 tcacctgtgg tacacgtgct gcatccagag cgacaccatt gccggagact tctgggacta
     2401 cggggtcatg ccttccaaca acggggtggt cacggccaac ctgcatgcgg acaccttccg
     2461 catcctggac tcgaccgagc atcctgacga ggccttcacg gccatgtact acctcctcac
     2521 cgaggcggct ctggacctgc tgacggccta cggtgccgcg ccggcggacc cggcgctgat
     2581 ggacgcttac ctggcagccc aagatgagag gtacccgcaa ggggtcaact ggcagaccat
     2641 cctcgatggc gccaactatg cggacaaccc gagccacgag accttcctcc ccggatggtc
     2701 ggagtacaag atccggctca aggagctcga aaccgggctc ctgtccaatc cagatctcga
     2761 tctggatgct gagatcgccc aggtggaaag cgacctgagt gcgatcttcg cggagaacgc
     2821 cgaaggctga cggttagggt cgatcgcatg actgtgatcg tcaatgaggg agcggccgag
     2881 cacccggccg ctccctcccg gcccgcgaga tcgtccctgg caaggagacg gggacgggcc
     2941 gggatttggt tcgtcatgcc ctggatcctc gggtttttcc tctggtacgc gatcccgatg
     3001 gcggcctccc tctggttctc gttcaccgac tacgacctgg tgagtgacga gccgatgcaa
     3061 ttcgtcgggc tggacaactg ggtgaggatg ttcaacgacg ccgacgttca ccagtcggtc
     3121 ttggtaaccc tcaagttcgg ggcgatcgcg ctccccgtcg cgattctgtt cccgatggca
     3181 atcgcatatt tgttggtcgc caaggccctc aaggggcggg agggttttcg cgccctgttc
     3241 tacctgccct cgatcatccc cttcgtagcc gcggtcctca tcttcaacgg ggtcttcaac
     3301 gggcaaatag gctgggtgaa caagatcctc gccattttcg gcatcgacgg gcccgattgg
     3361 ttccacgatg agttctggat ctacccatcc ctggtgttca tcggtctctg ggcggtgggc
     3421 aatgcgatcc tcatcttcat cgccgcgatg aaaggggtgc cgtctgatct ttatgcggcg
     3481 gcgcgcatcg acggcgccgg ggagtggggt tccttccggc acatcaccat ccccttcatc
     3541 accccgataa tcttctacaa cctggtcatc acactgatcg ggatcttcgg atacttcctg
     3601 gtccccttcg ttctcaacaa cggcagtggc gcccccaacg gggctaccca gttctatgcc
     3661 ctctacttct tcaggaccgg gttccagttt ttcgacatgg gatacgcggc gacattggct
     3721 tgggcgctgt tcatcgtcgc ggtgttgaca accggagccc ttttctggtc ggccaagtac
     3781 tgggtccact acgagttcga gggatgagac gatg
//
LOCUS       DASTXO010000076         4139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_777922, whole genome shotgun sequence.
ACCESSION   DASTXO010000076 DASTXO010000000
VERSION     DASTXO010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777922"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1750)
                     /locus_tag="VFU96_01490"
     CDS             complement(<1..1750)
                     /locus_tag="VFU96_01490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5111957.1"
                     /translation="MPIRPDKARERSRLDFNTPELTRIFRNELEERSGRLVAGAVALR
                     DHELDDGRIQDLIRDAHTIKGSAGLLGYDEIKEVASRLEHLWKQIGEGYRPPGDVVVA
                     MEASAGRLLPSLDVDDGELPALADKLIPGEWAQTLQGTVWSPPAEVIPLRRPEPGSLG
                     GLLTSVSDSLLGGSTRVDTGELYRLINRIVEVSLDSEALADLSLLSIEGTDPSLFRKS
                     WRQQLERLASSIAEIQDQAVALANIPFREATATFPQFVRYLGRRMGKDVRFELVGEDV
                     QLDRQIVDLLREPLRHLLVNAVDHGIEPSAARVTAGKKATGTVSVGAEIIDERVEVSV
                     SDDGAGIDWDRVADVATERAIPFVFSDLSPILFQPGFTTTEEVTDFSGAGDGLAAVRD
                     IVEKVNGVVHVESPEGMGTRVTMTLPLSMVLQNVVVVATGDQFWGLPEASIEAAMPLS
                     RAEISATDSGREVRFQSREVPCVSLAKAMGVTGAGDESELLVINTRFGLVAITVSELI
                     DRRRVAVKNLGPILEGPGHVTGAALLGGGQVLVVLDPNFLGYLAKRRPGLVGHRPRIL
                     VVDDSAGVRQLLSATLN"
     gene            complement(1799..3340)
                     /gene="guaA"
                     /locus_tag="VFU96_01495"
     CDS             complement(1799..3340)
                     /gene="guaA"
                     /locus_tag="VFU96_01495"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEU5111958.1"
                     /translation="MTHDAEPTVLVLDFGAQYAQLIARRVREGHVRSEIVSRDISPDE
                     VTRHNPVGLILSGGPASVYAEGAYRMDPALLEMGIPVLGICYGHQLIADLAGGTVTNT
                     GSAEYGNTDLTITKDSVLLRDLPKEQQVWMSHQDQVTEAPEGFLTVATSDGARVAAME
                     DRDRALFGVQFHPEVAHTPRGDEILRRFLYDVCGASPTWTSHSIIETQVEAIRAQVGE
                     ARVICGLSGGVDSAVAAALVHEAIGQRLTCVFVDHGLLRAGEAEQVEETFRSHFEVDL
                     IHVKAEDRFLELLAGVTDPEQKRRIIGETFIRVFEEAVGSIGDARYLVQGTLYPDVIE
                     SGSASAATIKTHHNVGGLPADMQFELVEPLRDLFKDEVRAIGEELGLPEEMVWRQPFP
                     GPGLAVRVIGEVTRERLELLRAADLIVTEEVRRAGLYRDIWQAFAVLPAVRTVGVQGD
                     LRTYAYPLVVRAVTSEDAMTADWARLPYEVLERISSRVINEVPGINRVAYDVSSKPPA
                     TIEWE"
     gene            complement(3375..>4139)
                     /locus_tag="VFU96_01500"
     CDS             complement(3375..>4139)
                     /locus_tag="VFU96_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HEU5111959.1"
                     /translation="VAAASLTPQRVLGWAPHALDAGLDVLVIQGTVVSAEHVSSREEP
                     LDLKQYIASCEVPVIVGGCASYSTALHLMRTGAVGILVGVGPGAACTTRGVLGVGVPQ
                     ATAIADAAGARIRYLDETGRYVHVIADGGMRTGGDVAKAIACGADAVMIGSPLASASE
                     APGRGYHWGMATFHPTLPRGARVEVGNRGSLEEILVGPAHENDGKLNLFGALQVSMAT
                     TGFSDVKEFQKAEVMIAPSLQTEGKSLQRNQRVGMG"
BASE COUNT          746 a         1402 c         1287 g          704 t
ORIGIN      
        1 cattgagtgt cgccgagagg agttggcgca cgccagcaga gtcgtcgacg accaagatcc
       61 ggggccgatg tcccaccaga ccgggccggc gcttggcgag atatccgagg aagttcgggt
      121 cgagcaccac caagacctgg ccacccccga gcagggcagc gcctgtgaca tgaccgggtc
      181 cttcgagaat cggcccgagg ttcttcacgg caacgcgacg tcggtcgatc agctcactga
      241 cagtgatggc aaccagtcca aaacgggtgt tgatgacaag cagttcggac tcatctcccg
      301 cgccggtcac ccccattgcc ttggccagtg agacacaagg gacctcccgg ctctggaacc
      361 tgacctctcg accgctgtcg gtagcactga tctcggcccg cgagaggggc atggcggcct
      421 cgatcgacgc ctcgggcaga ccccagaact ggtctccggt ggccaccacg accacgttct
      481 gcaggaccat ggagagtgga agcgtcatgg tgactctggt gcccatgccc tccgggctct
      541 cgacatggac gacaccgttg accttttcga cgatgtcacg caccgccgcc aacccgtcac
      601 cggcaccgga gaagtcggtc acctcctcgg tggttgtgaa gccgggctgg aagaggatcg
      661 ggctcaagtc ggagaagaca aacgggatgg cgcgttcggt ggccacatcc gccacccgat
      721 cccagtcgat gccggcgccg tcgtcggaca ccgagacttc gacccgctcg tcgatgatct
      781 cggcaccgac gctcaccgtg cccgtggcct tcttcccggc tgtcacccgc gccgcggagg
      841 gctcgatccc gtgatcgacc gcgttgacca ggagatggcg gagtggctcc ctgaggaggt
      901 cgacgatctg ccggtcgagc tgcacgtctt cgccaaccag ctcgaatcgc acgtccttgc
      961 ccattcgccg tcccaggtag cggacgaact gggggaaggt cgccgtggcc tcgcggaacg
     1021 ggatgttggc gagcgcgacc gcctgatcct ggatctcggc gatggaagag gccagtcgct
     1081 ccagctgctg acgccaggac ttacggaaca gcgaggggtc ggtgccctcg atcgacaaga
     1141 gggagaggtc tgcgagggct tcggagtcga gtgagacttc gacgattcgg ttgatgagcc
     1201 ggtagagctc gccggtgtcg acacgggtcg agcctccgag cagcgagtcc gacaccgagg
     1261 tgaggagccc acccaggctc cccggctcgg gccggcgcaa ggggatcacc tccgccggtg
     1321 gcgaccacac cgtgccctgg agcgtctgcg cccactcccc ggggatcagc ttgtcggcga
     1381 gagcaggcag ctcgccgtcg tcgacgtcta gcgaaggcag gagccgcccg gcgctggcct
     1441 ccatggccac tacgacgtcg cccggtgggc gatagccctc gccgatctgc ttccacagat
     1501 gctcgagccg ggaggcgact tcctttatct cgtcgtagcc gaggagaccc gccgaccctt
     1561 tgatggtgtg tgcgtcccga atcaggtcct ggattcgccc gtcatcgagc tcgtgatcgc
     1621 gcagggcgac tgcgcccgcg accaagcggc cggaacgttc ttccagctca ttgcggaaga
     1681 tccgcgtcag ctcgggggtg ttgaagtcca acctggatcg ctccctggcc ttgtcaggtc
     1741 ttatcggcaa aggaggccat gcactgaacg tgcctacccc ggtctctggg accgcggcct
     1801 actcccactc gatcgttgcc ggaggcttgc tcgagacgtc gtaggcgacg cggtttatgc
     1861 ccggaacctc gtttatcact ctgctcgata tccgctccag cacctcatag ggcaaccggg
     1921 cccaatcggc ggtcattgcg tcttccgagg tcaccgcccg cacgaccagc ggataggcat
     1981 aagtgcgcag atcgccctgg actcccacgg tacgcaccgc ggggagcacc gcgaaagcct
     2041 gccagatgtc tcggtagaga ccggctcgcc ggacctcttc ggtcacgatc aggtctgcgg
     2101 cccgcagcaa ctccagacgc tcccgtgtga cctcgccgat caccctgacc gcgagcccag
     2161 gaccggggaa cggctgacgc cacaccatct cctccggcag ccccaactcc tctccgatcg
     2221 ccctcacctc gtctttgaag aggtccctca aaggctcgac gagctcgaat tgcatgtcgg
     2281 cgggaaggcc acccacgttg tggtgtgtct ttatggtcgc tgcgctggcc gacccggact
     2341 cgatcacgtc cggatagagg gtgccctgca ccaggtagcg ggcatcgccg atcgagccga
     2401 ctgcctcctc gaacacgcgg atgaacgtct cgccgatgat cctccgcttc tgctccggat
     2461 cggtgacacc ggccaacaac tcgaggaagc gatcctccgc cttgacatgg atcaaatcca
     2521 cctcgaaatg cgagcgaaag gtctcctcga cctgctcggc ctctccggct cggagcaacc
     2581 cgtggtcgac gaagacacag gtcaaccgct ggccgatcgc ctcgtggacg agggcagcgg
     2641 cgacagccga gtcgacacca cctgacagac cgcagatcac ccgcgcctcc cccacctggg
     2701 ctctgatcgc ctcgacctgc gtctcgatta tcgaatggga tgtccaagtc ggagaagcac
     2761 cgcagacgtc gtagaggaac cgtctcagga tctcatcccc tctgggcgtg tgagccacct
     2821 cggggtggaa ctggacaccg aacagagccc gatcacgatc ctccatggcg gccacccgag
     2881 cgccatcgga tgtggcaacc gtgagaaacc cttcgggggc ctcggtcacc tgatcctgat
     2941 gcgacatcca gacctgctgt tccttgggta gatcccgcag gaggaccgag tccttcgtga
     3001 tggtgaggtc ggtgttccca tattcggcac tcccggtgtt ggtcaccgtg ccgccggcca
     3061 gatcggcgat cagctgatgg ccataacaga tgcccaggac ggggatgccc atctcgagga
     3121 gtgccggatc catccggtag gcgccctcgg catagacaga ggccggaccg ccggacagga
     3181 tgagacccac cgggttgtgt cttgtgacct cgtcggggct gatgtcccgg gagacgattt
     3241 ccgatcggac atgtccctca cggacccgac gggcgatcaa ctgtgcgtac tgggccccaa
     3301 aatccagaac caacaccgtt ggttctgcgt catgggtcat ggcgcagaac gaaagatctg
     3361 agacgtggcg ggcttcatcc cattcccacc cgctgattgc gttgcagcga cttcccttcg
     3421 gtctgtagtg agggagcgat catcacctcg gccttctgga actccttcac atcagagaaa
     3481 ccggtagtgg ccatcgacac ctgaagcgcc ccgaacaaat tgagcttgcc gtcgttctcg
     3541 tgtgccgggc caaccaggat ctcttcgaga ctccctcggt tgccgacttc gacgcgcgcg
     3601 ccgcggggga gtgtcgggtg gaaggtggcc atgccccagt ggtagccccg gccgggggcc
     3661 tcgcttgccg aggcgagtgg agaaccgatc atcaccgcgt ctgccccgca ggcaatggcc
     3721 ttggcgacat cgccgccagt cctcatcccg ccgtcggcga tcacgtggac ataacggccg
     3781 gtctcgtcca gataccggat ccgtgcccct gcggcgtccg cgatcgcggt tgcctgaggg
     3841 acacccacac caaggactcc cctcgtcgtg catgccgccc ccgggccgac gccgacgagg
     3901 atgccgaccg ccccggtgcg catcagatgc agtgccgtcg agtagctggc acagccacca
     3961 acgatcaccg gcacctcaca gctcgcgatg tactgcttca gatcgagcgg ctcctcgcgg
     4021 ctggacacgt gctcggcaga gacgacagtc ccctggatca caagcacgtc gagcccggcg
     4081 tcaagggcgt gaggcgccca gcccagcacc cgttgcgggg tgagactcgc cgcggccac
//
LOCUS       DASTXO010000077         3890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_41121, whole genome shotgun sequence.
ACCESSION   DASTXO010000077 DASTXO010000000
VERSION     DASTXO010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41121"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            397..1953
                     /locus_tag="VFU96_01505"
     CDS             397..1953
                     /locus_tag="VFU96_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEU5111960.1"
                     /translation="MALFRKKSPQIVRKSSTEGTVEEHKTVRVKGKKVEVTRVEGRPA
                     PVAKPKKDRRHQPPGKGGGNHSRNGVSTSRTRTRRPVDDEVILPVETGRKQMLVRVHR
                     GQTQMVILEGPVLVEHYVAREDAGSVAGNIYLAKVRNVLPGMEAAFLDFGAAKNGVLY
                     ASDVASDGKGNGRPNRRIENVLKEGDDVLVQVTKDAMGAKGARLTGVPSLPGRYLVLV
                     PDSDSIGISRRLPDDDRTRLREVINKVRPEGYGVIVRTAALHASAEELAADISRLRKV
                     WDQIGEESQNGGAPRLVHQEPDLLIKVIREHFTADFRKLLIDDARAYETVVDYLRGTA
                     PDLVSKVQHYTDGVPLFERYHVDDQIKKALDRKVYLPSGGHLVIDRTEALTVIDVNTG
                     KFVGSSNLEETVLQNNLEAAEEIGRQLRLRDIGGIIVIDFIDMETIDNQQSVIRRLKE
                     ALAKDKTRTQVFDVSHLGLVEMTRKNVSAGLLEQFSSKCEECGGRGVIVHEHAIAAED
                     LVVEDFSLAE"
     gene            2045..2344
                     /gene="rplU"
                     /locus_tag="VFU96_01510"
                     /pseudo
     CDS             2045..2344
                     /gene="rplU"
                     /locus_tag="VFU96_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
     gene            2571..2822
                     /gene="rpmA"
                     /locus_tag="VFU96_01515"
     CDS             2571..2822
                     /gene="rpmA"
                     /locus_tag="VFU96_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015287257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU5111961.1"
                     /translation="MSKTKAGGSSKNGRDSAAKRLGAKVFDGEFVNAGAILVRQRGTR
                     IHPGENVGKGSDDTLFATSPGTVKYGTRANRRLVSVLPE"
     gene            2837..>3890
                     /gene="obgE"
                     /locus_tag="VFU96_01520"
     CDS             2837..>3890
                     /gene="obgE"
                     /locus_tag="VFU96_01520"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEU5111962.1"
                     /translation="MFVDRVRVNLRAGNGGAGVVSFVRAKGKPKGKPTGGNGGPGGSV
                     YLRADPSVATLLRYQRNPHYTAGHGTHGQGDVRHGKTGEPLILPVPLGTVVIDDEGTM
                     VADLVEEGQVIEAVKGGRGGRGNAAFVNPSNRAPSVAEQGEYGRSEWLTLELKLLADA
                     ALIGFPNAGKSTFISKVSAAKPKIADYPFTTLVPNLGVVSFDGREFVMADIPGLIEGA
                     ASGKGLGHEFLRHVERARVLVILLDPSPMQERAPEEQLQILRREITDYSPEIAARPSI
                     VLLNKSDLAEMSVLAADLGASPVSAVSGEGIRESLHAIADLVDEAERIAPQRKGFVLH
                     RPLGPTFTVRREDGGWR"
BASE COUNT          797 a         1195 c         1243 g          655 t
ORIGIN      
        1 tgcggtgggc ccggcatgta cacccaacgg atcgtgcatt cagaggagct cgtggtgctc
       61 ggggcgcagg gcgcctacga ggctcggtgt cgggcgtgct acgacccgac cgagcctgag
      121 cagccggagc tcgatctcgg ggctggatag gttcagcgtt tcctttctgg taatctttgc
      181 tggtccgcca gacgcgacat cttcccctct tcggagtgtt tcgttgatga gcacccttgc
      241 ataccggtcg gtccgcacgt cagtccttga cgagcgcctc catgcagggt gggcccacca
      301 gcgcgatctg gcgcatctat tcgggcccga aagcccatgc caggaaatgg gcattcgggt
      361 cgactcaagc atcgactctc agaggagtaa caacacatgg ctctatttcg caagaagagc
      421 ccacagatcg ttcggaaatc ttcgacggaa gggaccgtcg aggagcacaa gaccgtccgc
      481 gtcaagggaa aaaaggtaga ggtcaccagg gtcgagggca gaccagcccc cgtcgccaaa
      541 ccgaagaagg atcgacgtca ccagccgccg ggcaagggag gcggcaacca ctcccggaac
      601 ggcgtttcca cctcccgcac caggacccgt cggcccgtcg acgacgaggt gatcctcccg
      661 gtcgagaccg ggcgcaagca gatgctcgtc agggtgcacc gcgggcagac tcagatggtg
      721 atcctcgagg gcccggtcct cgtcgagcat tacgtcgcac gtgaagacgc cggatccgtc
      781 gcagggaaca tctacctggc gaaggttcga aacgtcctcc ccggcatgga ggcggcattc
      841 ctcgacttcg gagccgccaa gaacggtgtc ctatatgcat cggacgtcgc ctccgacggg
      901 aagggcaacg gtcgccccaa ccgcaggatc gagaacgtcc tcaaggaggg cgacgacgtc
      961 ctggtccagg tgaccaagga tgcgatgggt gccaaagggg cacggcttac cggcgttcca
     1021 tcattacccg ggcgctatct cgtgttggtc cccgactccg acagcatcgg gatcagccgc
     1081 cggctgccgg atgacgaccg gacacgtctg cgagaggtga tcaacaaagt tcgccctgag
     1141 ggatacggcg tgatcgtgag gactgccgcc ctccacgcca gcgctgagga gttggctgcc
     1201 gatatctccc gcttgcgcaa agtctgggac cagatcggtg aggagtcgca gaacggcggg
     1261 gccccccgtc tcgtacatca ggagcccgac ctcctcatca aggtgatccg tgagcatttc
     1321 acggcggatt tccgcaagtt gctcatcgat gacgccaggg catacgagac cgttgtcgac
     1381 tatctgcgag gcacggctcc agatctcgtc tccaaggtcc agcactacac cgacggcgtc
     1441 cctctcttcg agcgctacca cgtcgacgac cagatcaaga aagccctcga ccgaaaggtc
     1501 tacctgccat cggggggcca ccttgtcatc gatcgcaccg aggctctcac cgtcatcgat
     1561 gtcaacacgg ggaagttcgt cggctcgtcg aacctcgagg agaccgtcct gcagaacaac
     1621 ctcgaagcag cggaggagat cgggcgtcag cttcggctgc gcgatatcgg cgggatcatc
     1681 gtcatcgact tcatcgacat ggagacgatc gacaaccaac agtcagtgat ccggcgtcta
     1741 aaggaggccc tggccaagga caagaccagg acccaggtct tcgacgtgtc tcatctcggg
     1801 cttgtggaga tgacgcggaa gaacgtgtcc gccgggttgc tcgagcagtt ctcctccaag
     1861 tgtgaggaat gtggtggtcg tggggtgatc gttcacgagc acgccatcgc cgcggaggat
     1921 ctcgtggtgg aggacttctc gctggctgag tagggctcgc tgcgcgtcgt agaggctgtc
     1981 tttgcggtaa acgggcgacc cggtaccatt gagtcccgat tcccttcttc caagaggttt
     2041 acccatgtac gccatcattc gcgccggcgg caagcaggcc aaggtgcaat cgggcgatgt
     2101 aatcgagatc gagcgggtga aaggtgacgc cgacaagctg tcctttgcac cgcttctcgt
     2161 ggtcgacgac aagggcaacg cggtgtccga tcgtgacgtc ctggccaagg cgtcggtgac
     2221 ggcgaaggtc ctcggcgagt cccagggcca gaagatcgac atcttcaaat acaagaacaa
     2281 gaccggctat cggcgtcgtc agggtcaccg tcagaagtac acgcagatcg agataaccga
     2341 gatcaaactg ccgggcgcca agtcctcggg cgccaagact gcggcaccca agccggggac
     2401 cgcgaagacg accgccgcga agacgacaac tccgaaggcg actgctgcaa agacgaccgc
     2461 ggcaaagtca acggccgcca agtcgaccgc cgccaagtcg accgccgcca aggcaacggc
     2521 tgcgaagacg aacaagcaag caaagaccga ctcgtcagag gaggagtgag atgtcaaaga
     2581 caaaagcagg cggttcctcc aagaacggtc gtgactccgc cgccaagaga ctcggagcca
     2641 aggtgttcga cggcgagttc gtgaacgccg gggccatcct ggtgcgtcag cgcgggacca
     2701 ggatccatcc cggtgagaac gtgggcaagg ggtccgacga cacccttttc gccacctctc
     2761 ccgggaccgt caagtacggg acccgggcca accggcgtct ggtcagcgtc ctgcccgagt
     2821 gaagtaactt ccggtcgtgt tcgtcgaccg ggttcgtgtc aacctccgcg ccggcaacgg
     2881 cggtgcgggg gttgtctcgt ttgtgagggc gaaggggaag cccaaaggca agccgacggg
     2941 gggaaacggc ggcccgggcg ggtcggtgta cctgagagcc gatccctcgg tggcgacgct
     3001 tcttcgctat caacggaacc cccactacac cgcaggtcat ggcacccatg gccagggtga
     3061 cgtcaggcac ggcaagaccg gtgagcccct gatccttccc gttccgctgg gcaccgttgt
     3121 catcgacgac gagggcacca tggtcgccga tctggtcgaa gagggtcagg tgatcgaggc
     3181 tgtgaagggc gggcgcggtg gtcggggaaa cgccgctttc gtcaacccgt ccaatcgggc
     3241 tccctccgta gccgagcaag gggagtacgg gcggtcggag tggctcacac tcgagctgaa
     3301 gctccttgca gatgccgccc tcatcggctt tcccaacgca ggcaaatcca catttatctc
     3361 caaagtgtcg gcggccaaac ccaagatcgc cgactaccca ttcacgacgc tggtgccgaa
     3421 tctgggcgtt gtcagctttg acgggcggga attcgtcatg gcggatattc cgggtctcat
     3481 cgagggagca gcgtcgggca aaggtctcgg ccacgagttc cttcgtcacg tcgagcgggc
     3541 tcgagtcttg gtcatcctcc tcgatccgtc gccgatgcag gaacgagcgc cggaggagca
     3601 gctgcagatc ctgagacgcg agatcaccga ctattcgcca gagatcgcgg cacggccgag
     3661 catcgtccta ctcaacaagt cggacctcgc cgaaatgagc gtcctcgcag ctgacctagg
     3721 cgcatcgccg gtttcggcgg tcagcgggga aggcattcgt gagtctttgc acgccatcgc
     3781 ggacctggtc gacgaagcgg agcggatcgc accccagcga aagggtttcg tgcttcatcg
     3841 ccccctgggc ccaacgttca cggtgcgacg agaagatggg ggctggcgca
//
LOCUS       DASTXO010000078         3145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_225403, whole genome shotgun sequence.
ACCESSION   DASTXO010000078 DASTXO010000000
VERSION     DASTXO010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225403"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="VFU96_01525"
     CDS             complement(<1..940)
                     /locus_tag="VFU96_01525"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU5111963.1"
                     /translation="MHPFAKDPDPSPIGQSLKAVRRSRVARPAVRESWLRDGPGSNRE
                     GRGHEPFVEVGWDRALDLVAAELERVRSTFGNKSIYGGSYGWASAGRFHHAQSQIHRF
                     LNAIGGYTYSVDDYSIAAGHVITPHIFGISFDDFMDEQPHFVEISENTELVLAFGGMP
                     IKNSQVQYGGQGRHMLSERLETCRDRGVEFINLSPLGEDLIEAVEAEWIPVRPSTDTA
                     VMLGLAHSLLVSGKHDEDFLQRYCVGWDRLRAYLLGEPDGVPKTADWASGISGVDADR
                     LRALAMDMAGRRTLITVSWSVQRNDHGEQPYWMAGAL"
     gene            complement(1100..1648)
                     /locus_tag="VFU96_01530"
     CDS             complement(1100..1648)
                     /locus_tag="VFU96_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111964.1"
                     /translation="MSSFNARLRLPGHSRLPLGVEVDILNERMTLTAGERTVANWPLD
                     QLEIVVRADGFHIRVDGEEMVLNVTDSKAFAAALGVEAAPARPIGAAAGQTSTSLRPT
                     MLNQPSDAELDDLRRRISAFARDLAANAVPPEEVFARWLRLLKEINRRLGEGSLPTSL
                     FYQLNTHLLELMPEPAPAPATV"
     gene            1824..2786
                     /locus_tag="VFU96_01535"
     CDS             1824..2786
                     /locus_tag="VFU96_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5111965.1"
                     /translation="MPPTRSPEIESVTRRVLLAFETGDMAAMANLVSADPSLRVLGFA
                     VDEWWTGPEEFLKIRETQIDEIPALHIQIEKVEAFEDGAFGWAAISTTVVTSETKTPM
                     RHTAVLRLEAGAWKVILWQNSVPVSNSQIFGVELTTTLDDLVSSVLDEHGPLSSVGLE
                     GTMTLVFTDIVDSTVLAETLGDTAWAELINRHESTIRQVTASQGGTVVKLLGDGSMLA
                     FESARAAVRAAVDIQRATESGPFGVRIGIHTGEVIRTADDLLGLTVNKAARVAAAADS
                     GTIMISSTTRDLVGSLEGLRIGEPKIVALKGLSDTHQIVPIEWE"
     gene            2783..>3145
                     /locus_tag="VFU96_01540"
     CDS             2783..>3145
                     /locus_tag="VFU96_01540"
                     /inference="COORDINATES: protein motif:HMM:NF024694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEU5111966.1"
                     /translation="MTAGDFLEPDVDPLMAAIGSQFVIQQHHATRLHHDVRLEMMSGD
                     TPVLVSWAVPKGLPRRRGDRHLAIRTPDHEMEWGTFSGSIGKGEYGGGEVRIFDHGDY
                     EMVGRTDDRLTFQLKGDRL"
BASE COUNT          567 a          980 c         1021 g          577 t
ORIGIN      
        1 ccagggcgcc ggccatccaa tatggctgct ccccgtgatc gtttcgctgc accgaccaac
       61 tgaccgtgat cagtgtgcgc ctccccgcca tgtccatggc gagcgcgcga aggcgatccg
      121 cgtcgacccc gctgatgccg ctcgcccagt ccgccgtctt cggcacgcca tcgggctccc
      181 cgagcagata ggcgcggagc cggtcccagc cgacgcagta gcgctgcagg aaatcctcat
      241 cgtgcttccc tgagacgagg aggctgtggg cgagcccgag catcaccgct gtgtcggtgc
      301 tgggccggac ggggatccac tcggcctcca cggcttcgat cagatcttcg cccagcggcg
      361 agagattgat gaactcgacc ccgcgatcac gacatgtctc cagcctctcg ctgagcatgt
      421 gacggccctg accgccgtac tggacctgac tgttcttgat cggcatgccc ccaaacgcca
      481 acaccagctc ggtgttctcc gagatctcga cgaaatgggg ctgctcgtcc atgaagtcgt
      541 cgaaggagat cccgaagatg tgaggcgtga tcacatgccc ggccgcgatc gagtagtcgt
      601 cgaccgagta ggtgtatccg ccaatcgcgt tcaggaagcg gtggatctgg ctctgtgcgt
      661 ggtggaacct cccggcgctg gcccagccat agctaccccc atagatcgac ttgttgccga
      721 aggtggagcg gacccgctcg agctcggcgg cgaccaggtc gagggcgcgg tcccatccca
      781 cttcgacgaa tggttcgtgt ccccgacctt ctcgattcga gccggggccg tcacggagcc
      841 acgattccct gaccgccggc cgcgcgacgc ggcttcttcg cacggctttc agcgattgac
      901 cgatcggaga tgggtcgggg tccttggcga aggggtgcac cgcggtgatc tcgcggccat
      961 cggtctcgac ctcgtaggcg ccccagttgg tgagcgtgcg atgtctggtg gtagtcgttt
     1021 ctcgcctccc cgaagacccg cgacgttagc ggcccttggg gttcgacccc tttcccatgc
     1081 caggcagcgg ctgcttcggc tagacggttg ccggcgccgg cgccggctcg ggcatcagct
     1141 cgagcaagtg cgtgttcaac tgatagaaca gagaggtcgg cagtgagccc tcgccgagac
     1201 gcctgttgat ctccttgagc agtctcaacc accgggcgaa tacctcttcg ggcggcaccg
     1261 cgttcgcggc aaggtccctg gcgaaagcgg atatccgacg tcgaagatca tccagttcgg
     1321 cgtcactcgg ttgattcaac atggtcggac ggagcgacgt gctcgtctgc ccggcagccg
     1381 caccgatcgg gcgagccggg gcggcctcga ccccgagagc ggcggcgaag gctttcgagt
     1441 cggtgacatt gaggaccatc tcctcgccgt cgaccctgat gtgaaaacca tcagcgcgga
     1501 caacgatttc cagttggtcg agcggccagt tcgcgacggt tcgctcaccg gcagtcaagg
     1561 tcatccgctc gttgagtatg tccacctcca caccgagcgg aagacgggaa tgacccggca
     1621 ggcgtagcct ggcgttgaac gacgacaaat tctccctcca caggtctcgg aggggcggag
     1681 gccccgatcc agcttgcctc atgtaatcgt ctggagtggt taggtggata ctcgtctcac
     1741 agcgattgtt ggggattgtc gacgggggag gatgccaccg tcccggcagg cgcgagcaga
     1801 ccggtgaata cactcgtcga gtgatgcctc ccactcgatc gccggagatc gagtcagtca
     1861 cgcgcagagt gctgctcgca ttcgagaccg gggacatggc cgcgatggcc aacctggttt
     1921 ccgcagatcc cagcttgcgc gtcctcggat tcgctgtgga cgagtggtgg acggggcctg
     1981 aggagttcct caagattcga gagactcaga tcgacgagat tcccgccctt cacatccaga
     2041 tcgagaaggt cgaagccttc gaggacgggg ctttcggatg ggcggccatc tccacgacgg
     2101 tcgtcacgtc tgagacgaag acaccgatgc ggcacactgc cgtgctgaga ctcgaggccg
     2161 gggcatggaa ggtgatcctt tggcaaaact cggttcctgt ctccaacagt cagatcttcg
     2221 gggtcgagct caccacgaca ctggacgatc tcgtgagctc ggtcctggac gagcacggcc
     2281 ctctctcctc cgtcggtctc gaggggacga tgaccctggt gttcaccgac atcgtcgact
     2341 cgaccgttct cgccgagacc ctcggagaca cggcctgggc tgagctgatc aaccgacatg
     2401 agtccacgat ccggcaggtg acagcgtcac aaggaggaac cgtggtcaag ttgctcggtg
     2461 acggctcgat gctggcattc gagtcagccc gcgccgcggt acgtgcggcg gtggacattc
     2521 agcgggcaac ggagagcggc cctttcgggg tccggatagg cattcacacc ggggaagtga
     2581 tccggacggc cgacgatctg ctgggattga cggtcaacaa ggcggccaga gtcgccgcag
     2641 cggcggattc cgggaccatc atgatctcat cgaccacgag ggacctcgtg ggctcgctcg
     2701 aagggctcag aatcggtgaa cccaagatcg tcgctctgaa gggcctctcg gacacgcatc
     2761 aaatcgtccc catcgaatgg gaatgacggc gggggacttc ctcgagccag acgtcgatcc
     2821 gttgatggcg gcgatcggat cccagttcgt gatccagcag catcacgcca cccggctcca
     2881 tcacgacgtc cggttggaga tgatgagcgg ggacaccccg gtgctggtct cctgggcagt
     2941 gcccaagggt ctcccccgac ggcggggaga tcgtcatctc gctatccgta cgccggatca
     3001 cgagatggag tgggggactt tctccgggtc gatcggtaaa ggggagtatg ggggtgggga
     3061 ggtgcggatc ttcgaccacg gtgattacga gatggtgggg cgaaccgatg accgtctgac
     3121 cttccaactg aaaggcgacc gtctc
//
LOCUS       DASTXO010000079         6659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_667844, whole genome shotgun sequence.
ACCESSION   DASTXO010000079 DASTXO010000000
VERSION     DASTXO010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6659
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667844"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VFU96_01545"
     CDS             complement(<1..697)
                     /locus_tag="VFU96_01545"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0106411 - XMP 5'-nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEU5111967.1"
                     /translation="MSDLPRVIVTNDDGIDSPGLRLLAAGLASDFDVVVAAPASDMSG
                     SGTGIGGFDPVKGVDLTTVDMGGITAYTIAGPPGLAVMAAALGAFGRFPDLVVSGINA
                     GMNTGHSIIHSGTVGAALTARTFRSRGMAISLAQSDPWRWETAVEVGVSAAKWLLGRS
                     GSPYVLNVNVPGVPLAEVNGIHWADLDEFGYFRVANADVEDRKLQFVVGSSSAGTDPA
                     SDTALCAKNFVTMT"
     gene            complement(694..1641)
                     /locus_tag="VFU96_01550"
     CDS             complement(694..1641)
                     /locus_tag="VFU96_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEU5111968.1"
                     /translation="MAHEDHTIAIFAPHLTLTVTIEAGREDGPDDIHIHPGGQGFWVA
                     RMLRHLGEKPLLCGPVGGEPGGVLRNLINQWGIDLSPLEVQESSPAIVQDRRSGDRAS
                     IAVAPAPSLSRHELDDAYGRILDHALSSEVVVITGQSSEIVPVNTYRRLGHDLASADV
                     PVVGDLHGDELEAFLDGGQLDLLKISDEDLIEDGVLDGEDEDMALDQLHRLRESGAKR
                     VVISRGPRPAVALIEGVTYRITGPDLEPADYRGAGDSMTAGLTAGLRRGLEPEAMLRL
                     ASGAGAANVTRHGLGSASDDLIPRLAERVLIEVLTPLTR"
     gene            complement(1648..2037)
                     /locus_tag="VFU96_01555"
     CDS             complement(1648..2037)
                     /locus_tag="VFU96_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111969.1"
                     /translation="MHDLTGTVVAIIDDAQEGHAAVAALAQQQFTAELLHGEEGRAHL
                     NEDDEKGLSAAAKKLALAFGDEVRIIDRLDKALENGRSVVSVDVDDDDDDAARVAVIL
                     EEHGGHDMWRLGEWSFNRIGPEGSGEG"
     gene            complement(2089..3171)
                     /locus_tag="VFU96_01560"
     CDS             complement(2089..3171)
                     /locus_tag="VFU96_01560"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5111970.1"
                     /translation="MADPPSYDSRGLVNLVAEIESRLIGSSPSPRLDPEIAESIPEGA
                     TYVVVLFDGLGIAQLDHDTARSLRESLRATLHAPFPSTTSVALATVATALPPSQHGLT
                     SHLCWLEETGKVVNTLKWVDLSGHPVHLDYGSFLPGPNLWERLRRAGVEPITVQPGAF
                     AGSPLSRLVYRGARFERTWDDHDLIDATVQLATEPNRLIFTYFWPVDFAGHVHGLESR
                     EFTDALRLAAHLWEEVVSRSPPGVTVVGTADHGLLDYADEDKLLVRDPRFQSLRYAGD
                     PRGVHVWGEDSLFAVLAEVTGAEITDPLGLLGPDPGPAARARVGDALMLAPPGKVILP
                     PGFDKRLRSYHGGLAVEEVEIPLLVG"
     gene            3292..3657
                     /locus_tag="VFU96_01565"
     CDS             3292..3657
                     /locus_tag="VFU96_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003974348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEU5111971.1"
                     /translation="MSSYFESYLTVGAFAVLGAILVMVMLGVAAVLRPTNPTTTKRLT
                     YECGVDPVGSGWSQSYVRYYVYGLLFVIFDVEAVFIFPWAIVLEELGTFGLVEMFIFI
                     DILILGLVYAVRKGVLRWE"
     gene            3648..4145
                     /locus_tag="VFU96_01570"
     CDS             3648..4145
                     /locus_tag="VFU96_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEU5111972.1"
                     /translation="MGVVQKFGAPKPVSWLLNWSRKYSLWLFQFGLACCAIELMAASG
                     PRYDFMRFGIIPLPASPRQADLMVVAGTVTDKMAPAIRRLYDQMPDPKYVISMGSCAN
                     CGGPYWDSYSVTKGVDQVIPVDVYVPGCPPRPEALMDGIVLLQKKIQGEDIKEKWSRR
                     DLEPV"
     gene            4126..4764
                     /locus_tag="VFU96_01575"
     CDS             4126..4764
                     /locus_tag="VFU96_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEU5111973.1"
                     /translation="MTSSPSETEAAAETVEAPTVHPLQEYADRVAAAVEGTATITFDT
                     VKVEVPADRWVSALTTARDDLGLVFFSWLSAVDWASEVEVGDPFPGEVEDRFEVLATL
                     GDVSEGRRVTFSTTLSKDSPSIASLVDVFAGANWHEREAYEMFGIDFEGHPNLAHLYL
                     PDSFQGNPLRKNFPLLSREVKPWPGTVDVEAMPGSGDEEEPADSPSEENPEA"
     gene            4769..5956
                     /locus_tag="VFU96_01580"
     CDS             4769..5956
                     /locus_tag="VFU96_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D 1"
                     /protein_id="HEU5111974.1"
                     /translation="MLDQDLVRHLSESSVSVELQTEDMTLNIGPQHPSTDGVLRLVAR
                     VDGERAFDVQPVIVYMHRGYEKLSEVRTYPQITTLVNRIDWVAGYANEVPFIVAAEKL
                     LGIEVPERAQFIRLILTEMARISSHLIFMASYPLELGAATPLFFALRERERVLDLLEG
                     VTGGRFHPNFNRIGGVKPAYGSGSSTRKVIQDLPAGFLAETRDAMDKVLEAVDELEQL
                     VADNEIILARTKGIGILPPEIAAAYGVSGPNLRASGVKFDLRKVEDYLPYDRFDFEIP
                     VGQNGDCWDRWHVRLLEIRQSVRIVQQAIDGIPSGPLQAKVPKVIKVPQGETYVRAEN
                     PKGEMGYYLVSNGNQGPYRLKIRSASFSNISILPWILEGVLVPDIIAIMGSLDFVLGD
                     VDR"
     gene            5953..>6659
                     /locus_tag="VFU96_01585"
     CDS             5953..>6659
                     /locus_tag="VFU96_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HEU5111975.1"
                     /translation="MTQFISDLFSNFWVSLTGKLLLVALMIPLIGMVIGYAEMKLSAK
                     MQARVGPYFAGGRWGWAQLIADGVKFFQKEDLVPDEADRPVFKIAPVLVLVSTVAVLV
                     IIPFGPGLIFRDLEVGIFFALAISSLSTVGVLMAGWSSGNKYSLMGGLRAAGQLIAYE
                     LPLVLAVIGTVMLAGTMSMSGIVDAQISAGYPYVILQFIGFGIFMIAALAELSRIPFD
                     MPIAESELVMGYLTEYSG"
BASE COUNT         1121 a         2140 c         2175 g         1223 t
ORIGIN      
        1 gggtcatggt tacgaagttc ttggcgcaca aggcggtgtc acttgctggg tcggtcccgg
       61 ccgaggagga cccgacgacg aactgcagct tccgatcctc cacgtccgcg ttggcaacac
      121 ggaaataccc gaactcgtcc aggtccgccc aatggatgcc attgacctct gcgagcggga
      181 caccggggac gttcacgttg aggacatagg ggctcccgct ccggccgagg agccactttg
      241 cggcggagac accaacctcc acggcggttt cccaccgcca tggatcggac tgggccaggc
      301 ttatggccat gccgcggctg cgaaaggtcc gggcggtcag agccgcgccg acggttccgg
      361 agtggatgat cgagtgaccg gtgttcatgc cggcgttgat ccctgacacg acgaggtcgg
      421 ggaagcggcc gaatgcgccg agggccgcag ccatcacggc gagcccgggg ggcccggcga
      481 tggtgtatgc ggtgattcca cccatgtcaa cggtggtcag gtcgacgcct ttcaccggat
      541 cgaacccgcc gatccccgtg ccgctgccgg acatgtcgga agccggcgcg gcgacgacga
      601 catcgaagtc ggacgcgagg cccgcggcga gcagcctcag gcccggggaa tcgatcccgt
      661 cgtcgttggt gacgatcact ctggggagat cgctcatcgc gtgaggggag tgaggacttc
      721 gatcaacacg cgctcggcga gacggggaat gaggtcgtcg gaagcgcttc ccagcccgtg
      781 gcgagtgacg ttggctgccc ctgccccgga ggcgagacgg agcattgcct cgggctcgag
      841 gccgcgccgc aacccagcag tcaggcccgc ggtcatggaa tcgccggcgc ctcgatagtc
      901 cgcgggctcc aagtcaggac cggtaatccg ataggtcaca ccctcaatca gcgccacagc
      961 cggacgcggg cccctggaga tcacgactct cttggctccc gactctcgaa ggcggtggag
     1021 ctggtcgagc gccatgtcct cgtcctcccc gtcgagcact ccgtcctcga tcaggtcttc
     1081 gtcgctgatc ttgagcagat cgagttggcc cccatccaga aatgcctcga gctcatcgcc
     1141 gtgcagatca ccgacgaccg gcacgtcggc cgacgcgagg tcgtggccga gtcgccgata
     1201 ggtgttcacg gggacgatct ccgagctctg gccggtgatc acaacgacct cggagctgag
     1261 cgcatgatcg aggatccttc cataggcgtc gtcgagctcg tgacggctca ggcttggtgc
     1321 cggagccacg gcgatggatg cccggtcacc ggatcggcga tcctggacga tggcggggga
     1381 gctctcttgg acctccagcg ggctgagatc gatgccccac tggttgatga gattgcgcag
     1441 gacgccaccg ggctcacccc cgacggggcc gcacaggagc ggcttctccc cgaggtgtcg
     1501 aagcatgcgc gccacccaga agccttgacc cccgggatgg atgtggatgt cgtcggggcc
     1561 atcctcacgt cccgcctcga tggtcacggt gagggtgaga tgcggcgcga agatggcgat
     1621 ggtgtggtct tcgtgagcca tgtcggctca gccctctcca gagccttcag gcccgatccg
     1681 gttgaagctc cattcaccaa ggcgccacat gtcgtgcccg ccgtgctctt cgagtatgac
     1741 cgccacccgg gccgcgtcgt catcgtcatc gtcgacgtcg actgagacga cggatcgacc
     1801 gttctcgagc gccttgtcga gacggtcgat gatgcggacc tcgtcgccga acgcgagagc
     1861 gagtttcttg gccgctgccg agagcccctt ctcgtcgtct tcgttgagat gcgctctccc
     1921 ctcttcgccg tggagcagct cggctgtgaa ctgctgctgg gcgagcgcgg ccacggcagc
     1981 atgcccctcc tgggcatcgt cgatgatcgc cacaactgtc cctgtcaagt cgtgcatggg
     2041 cggattccac ctacccctcg acaagcaggg ccaaaccggg gtggtggctc agccgacgag
     2101 cagcgggatc tcgacttcct cgacggccag ccctccgtgg tagctccgga gacgcttgtc
     2161 gaacccaggg gggaggatga ccttgcccgg gggagcgagc atgagcgcat cccctacccg
     2221 ggcgcgggcc gccgggccgg ggtctggccc gaggagaccc agcgggtcgg tgatctcggc
     2281 gccggtcacc tcggccagca cggcgaacaa ggagtcttct ccccagacgt gaactcctct
     2341 gggatcgcct gcataccgca aagactggaa tcgcgggtct cgaacgagga gcttgtcctc
     2401 atcggcgtag tccaggaggc cgtggtcggc tgtacccacg acggtgacgc cgggaggcga
     2461 cctcgacacc acctcttccc agaggtgggc ggccaggcga agggcatcgg tgaattccct
     2521 cgactcgagc ccgtgaacat gtccggcgaa gtcgaccggc cagaaatagg tgaatatcag
     2581 acggttcggc tcggtggcca gttgaactgt tgcgtcgatg aggtcgtggt cgtcccaggt
     2641 ccgctcgaag cgggccccgc ggtagacgag tcgggacagg gggctgcccg cgaacgcccc
     2701 cggctgcacg gtgatcggct cgactccggc ccggcgcagg cgttcccaga ggtttggccc
     2761 aggtaggaag gagccatagt cgagatgcac cggatgcccg gagaggtcca cccatttcag
     2821 ggtgttgacg actttgcccg tctcctcgag ccagcagaga tgcgaggtca gcccgtgctg
     2881 cgaagggggg agggcggtgg cgaccgtggc cagggcaacg ctcgtggtgc tgggaaacgg
     2941 tgcgtggagg gtcgcgcgca gagattcgcg cagcgatcgc gccgtgtcgt ggtcgagttg
     3001 ggcgatgccc aggccatcga acagcaccac gacataggtg gccccctcgg gtatcgactc
     3061 ggctatctcg gggtcgagac ggggagaagg tgagcttccg atcagcctcg actcgatctc
     3121 ggcgacgagg ttcaccagac cgcgggagtc gtaggagggg gggtccgcca tcgatgcagg
     3181 ctagggcctg ccccaccatt tttgcctata ccagttgggg tttccaaact tcgacgtgaa
     3241 acgacccgtg cgtatactcg cccgcggtgg tgtccttcga ccctcaaagg catgagctct
     3301 tactttgaga gctatctgac ggtcggcgct ttcgccgtgc tgggcgccat cctcgtgatg
     3361 gtgatgctcg gcgtggccgc ggtcctccgc ccgacgaatc ccacgaccac caagcggctc
     3421 acctacgagt gcggcgtcga cccggttggg tccgggtgga gccagagcta cgtgcgctac
     3481 tacgtgtacg gcctgctctt cgtgattttc gacgtcgagg cagtcttcat ctttccctgg
     3541 gccatcgtgc tcgaggagtt gggcaccttc ggcctggtcg agatgttcat cttcatcgac
     3601 atcctcatcc tggggctggt ctacgccgtc cgcaagggag tgctccggtg ggagtagtcc
     3661 agaagttcgg ggccccgaag ccggtgagct ggcttctcaa ctggtcccgg aaatactccc
     3721 tctggctgtt ccagttcggt ctcgcctgtt gtgccatcga gttgatggcg gcgtccggcc
     3781 cgcgttatga cttcatgcga ttcgggatca tcccgttgcc cgcctccccg cgccaggccg
     3841 acctcatggt cgtcgcgggc acggtgaccg acaagatggc gccggccatc cgccgcctct
     3901 acgaccagat gcccgatccg aagtacgtca tctcgatggg ctcctgcgcc aattgtggcg
     3961 gcccctactg ggactcgtac tcggtcacca agggtgtcga tcaggtgatc ccggtcgatg
     4021 tctatgtgcc gggctgcccg ccacgtcccg aggcgctcat ggacgggatc gtcttgcttc
     4081 agaagaagat ccagggcgag gacatcaaag agaagtggag ccggcgtgac ctcgagcccg
     4141 tctgagaccg aggccgccgc cgagaccgtc gaggccccga cggtgcatcc gcttcaggaa
     4201 tacgccgacc gcgtcgcagc ggccgttgaa gggaccgcca cgatcacgtt cgacacggtg
     4261 aaggttgagg tgcccgcgga tcgatgggtc tctgccctga ccacggcacg ggacgacctc
     4321 gggctcgtgt tcttctcctg gctctctgct gtggactggg cctcggaagt cgaggtgggc
     4381 gaccccttcc ctggagaagt ggaagaccgc ttcgaggtgc tggccacgct cggagacgtg
     4441 agcgagggcc gcagggtcac gttttcgacc accctctcca aggactctcc gtcgatcgcc
     4501 agcctcgtag atgtcttcgc cggcgccaac tggcacgagc gcgaggcata cgagatgttc
     4561 gggatcgact tcgagggcca ccccaatctc gcccacctct atctccccga ctcgttccag
     4621 ggcaaccccc ttcggaagaa ctttccgttg ctcagccgag aagtgaagcc ctggccaggg
     4681 acagtcgacg tcgaggccat gcctgggagt ggcgacgagg aagaacccgc cgacagcccg
     4741 tcggaagaga acccggaggc ctgacgtggt gctggaccaa gacctggtgc gccatctctc
     4801 cgagtcctcg gtctcggtgg agctgcagac cgaggatatg actctcaaca tcgggcccca
     4861 gcatccctcg accgatgggg tgctgcgcct ggtggcccgg gtcgacggcg agcgcgcctt
     4921 cgacgtccag ccggtgatcg tgtacatgca ccgcggttac gagaaactgt ccgaggtcag
     4981 gacttatccg cagatcacca ctctggtcaa tcgcatcgac tgggttgccg gctacgccaa
     5041 cgaggtcccc ttcatcgtgg ccgccgagaa gctgctgggc atcgaggtgc cggagagggc
     5101 ccagttcatc aggctgatcc tcaccgagat ggcccgcatc tcgtcccatc tcatcttcat
     5161 ggcctcctat cccctcgagt tgggcgccgc caccccactc ttcttcgccc tgagggagag
     5221 ggagcgagtg ctcgatctgc tcgaaggggt caccgggggc cggttccatc ccaacttcaa
     5281 ccggatcggg ggggtgaagc ccgcctacgg gtcgggttct agcacgcgca aggtgatcca
     5341 ggacctgccg gccggtttcc tcgccgagac ccgcgacgcc atggacaagg tcctggaggc
     5401 ggtcgacgag ctggagcaac tggttgccga caacgagata atcctcgctc gcaccaaggg
     5461 gatcggcatc cttcctccgg agatagccgc cgcatacggg gtctcaggtc ccaatctccg
     5521 ggcctccggg gtgaagttcg acctgcgcaa ggtcgaggac tacctgccct acgaccgctt
     5581 cgatttcgag atcccggtcg ggcagaacgg tgattgctgg gaccgctggc atgtccgtct
     5641 cctcgagatc cgacagtcgg tccgcatcgt gcaacaggcc atcgacggga tcccatctgg
     5701 gccactgcag gccaaagtgc ccaaggtgat caaggtcccc cagggcgaga cctacgtccg
     5761 ggcggagaac ccgaagggag agatgggcta ctacctggtc tccaacggca accaggggcc
     5821 gtatcggctc aagatccgct ccgccagctt ctccaacatc tccatcctgc cctggatcct
     5881 ggagggggtc ctggtccccg acatcatcgc gatcatgggt tcactggatt ttgtcttggg
     5941 agatgtggac agatgaccca gttcatctcc gacctcttct ccaacttctg ggtgtccctc
     6001 accgggaagc tcttgctcgt tgctctcatg atcccgctga tcggcatggt catcggctac
     6061 gccgagatga agctgtcggc gaagatgcaa gccagggtcg gcccctactt cgccggtggc
     6121 cggtggggct gggcgcagct cattgccgac ggagtcaagt tcttccagaa ggaggacctc
     6181 gtccccgacg aggccgaccg ccccgtgttc aagatcgccc cggtcctcgt gctggtcagc
     6241 acggtcgccg tgctggtcat catccccttc gggcctgggc tgatcttccg cgacctcgag
     6301 gtgggaatct tcttcgccct cgccatctcc tctctgagca ccgtcggagt cctgatggcg
     6361 ggatggtcgt cgggcaacaa gtactccctg atgggcgggc tcagggcggc gggtcagctc
     6421 atcgcctatg agttgccact ggtgctggcc gtgatcggca cggtcatgct ggccgggacc
     6481 atgagcatga gcgggatcgt cgacgcccag atctctgccg gttatcccta cgtgatcctg
     6541 caattcattg gtttcgggat cttcatgatc gccgcgctcg ccgagttgag ccggatcccg
     6601 ttcgacatgc ccatcgccga atccgagttg gtgatgggat atctcaccga gtattccgg
//
LOCUS       DASTXO010000080         6326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_372917, whole genome shotgun sequence.
ACCESSION   DASTXO010000080 DASTXO010000000
VERSION     DASTXO010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6326
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372917"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..366
                     /locus_tag="VFU96_01590"
     CDS             124..366
                     /locus_tag="VFU96_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111976.1"
                     /translation="MIFAVAIAATMTAMVAGHPAIAEVLGGAVIVLVAVGLGSRITQR
                     AAVEVDSARVLTLDLVLILVVLLGLAGVAVVGLALP"
     gene            549..2834
                     /locus_tag="VFU96_01595"
     CDS             549..2834
                     /locus_tag="VFU96_01595"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU5111977.1"
                     /translation="MSVKQTLFGRPFATHEEEHQLLPKRLALPVFASDPLSSVAYATE
                     EAMLVLALAGTAAFSLLTPISLAIAALLLIVVISYRQTIKAYPDGGGAFIVANDNLGI
                     RTGAVAAAALLIDYVLTVSVSVAAGVAAITSAVPGVIPWRIEVAVGFVIILTVANLRG
                     VKEASTLFAAPTYLFVATVAVMLVAGFAQCLDGECPKAISSGTEVEAEVAVVGLFLIL
                     RAFASGSTALTGVEAVANGVQAFRQPKAHNAATTLGIMGAISITMFLGISTLALWFDV
                     RISEQTIDEYGTVISQIGRAAFNGGIGFYVLQVFTAAILILAANTAYQDFPRLSAILA
                     RHKLMPRQYLNRGDRLVFSNGIITLALIAVALLIVFDAEVSRLIQLYVVGVFTAFTLS
                     QIGMVRHWLKERDGGWRRSVLINTVGALTTGVVLVVVATVKFAHGAWIVVLAIPIFVV
                     VMLSIRRHYRGVAAQLRLIPVDAEPRPTRILVLIAHHDEGTDRALQYAEMVRADAITC
                     VHAEEPSSDDLVYTWDAAHPVHALEIISGEKDSISRRIVNRVRKERDLHPGAMITVIL
                     ADRVATRSILAPFAHRHSLGIKSRLLFERDVVVTDLNVLRRSRHRRLTPVPVSRVEQV
                     VLVSDMTRPIREALTYAQRLGPSVTALHIDVDSAQRDRLERQWAEAGYEFPLVVIPSD
                     FRSIVDPLVQYLRQQRRAASPGTLISAVIPEFVVPGRITQLLHNQTGLAIKGILAGES
                     GIAVTSVPFHLQPNGHGSRPQ"
     gene            2901..5444
                     /locus_tag="VFU96_01600"
     CDS             2901..5444
                     /locus_tag="VFU96_01600"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5111978.1"
                     /translation="MSRGKLKVFLGAAPGVGKTYAMLDEGRDLQSAGRDVVIGFVEAH
                     GRGDTIAMSADLEVVPPRWIVAESEASVEEMDVEALLARCPEVALVDELAHTNAFGSK
                     YPKRYQDVDVLLEAGIDVLTTVNIEHLESLNDVIFEITAVAQPDTIPDGVVRRADQIE
                     LVDLTQEGVRERLAAGKIFPAEQIDATLANYFRPGNLGALRELALSWTAERVDEAVTT
                     YRTVHGIDRPWETRERVVVALAGTDGGEAVVRRAARLAMRSRADLVGVHVRPTRGVIQ
                     GSDIALTSQRDLLQRLGGRYHEVVGDDVGSSLLAFAKEENATQIVLGASRRSRVDEFI
                     RSSPVARVIRDASDVDVHVISHEGMRRQRSSFRLPKKSPIGWSRRATAWAVAVLGLPL
                     LTWLLLRRGERLELQNVLLVYLLVAVVTGAIGGALPAVVAAIGGFFLANWFFTTPVET
                     FTIANPDQLLSLFAFLFVAATVGLLVGYSSRRSAEARTARAQAEALAATTTLAHPVFA
                     NDYEALLRSVRDALSLDAVSLVRRSADGWETLAWEGAVELKTPPDGSESIELAGDIVF
                     VLRDGRLTSDDRIVLRAFVAQMVQAIEREELLREARAAEGMAETDRLRTALLSAVSHD
                     LRTPLATIKASLTSLIETGVEWTPEQTLEFLNTALEETERLNRLVGHLLDASRLQANA
                     VHVLFRPVGLDEVVASALAGLRSKTDRVTVDISESLPEVNTDPDLMERVIANLIENAV
                     TWSPPDEMVRITAGEVQERIDLRIMDRGPGIPAASSDTIFQPFQRTGDSPDRDGVGLG
                     LAVAKGLLEAMGNDLVIEDTPGGGTTMVIALAAASALDGVAHEGATIGG"
     gene            5437..6150
                     /locus_tag="VFU96_01605"
     CDS             5437..6150
                     /locus_tag="VFU96_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014048809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5111979.1"
                     /translation="MASKQRILVVDDEAQILRALGINLKALGYQVDTAASGEEALRKA
                     ADHRPDAVILDLGLPGIDGIEVIRGIRGWSSVPIIVLSVRDAEREKIAALDAGADDYV
                     TKPFGMGELLARLRAALRREDGQEGELPVVTTDDFSIDLSTKRVTRATGDEIKLTPTE
                     WGIVEMLVRHQGKLVSQSQLLKEVWGPQYGDETNYLRVFMAQLRRKLEPQPSLPRYFI
                     TEPGMGYRFELAEEVGASD"
BASE COUNT         1134 a         1900 c         2045 g         1247 t
ORIGIN      
        1 caagactgcg ctgactgtgt agacgtgggc gccctttcct cgtcaagttc gtgctgggtt
       61 ccgcgtccct gcggtatcca ttggaacatg atgattggtc ggagatctgg ccttcggtcc
      121 cccgtgattt tcgcggtggc gatcgccgcc accatgaccg cgatggtggc gggacatccg
      181 gcaattgcgg aggtgctggg gggtgctgtc atcgttttgg tggccgtcgg actcggctcg
      241 cggatcacgc agcgcgctgc agtagaggtc gattcagcga gagtgctgac cttggacctg
      301 gtgctgatcc ttgtcgtctt gcttggcctt gcgggtgtgg ctgtggttgg cctggcgctt
      361 ccctgaatcg accggtcata tgtcgtcatc aggatcgggg ggacgggcga taagacggcg
      421 ttaggagacc tttcttacga tcgccgtagc cctatcaatg ggtgagtgcg cgtgtgtctg
      481 aacggcgcgg tttcttgcag gggtcgaaac cctcgtgctg gcccgacaac gaggggtgtc
      541 aatcggaatt gagcgtcaag cagacactgt ttgggagacc ctttgccacg catgaggagg
      601 agcatcaact gctgccgaag cgtctggcgc ttcccgtatt cgcatccgat cccttgtcgt
      661 ccgtggcgta tgccacagag gaggccatgc tggtactggc gctcgccggc accgccgcct
      721 tcagtctcct gaccccgatc tcgttggcaa tcgcagctct gcttctgatc gtcgtcatct
      781 cctatcgaca gacgatcaag gcctatcccg acggaggtgg cgcgttcatc gtcgcgaacg
      841 acaatctggg tatccggacc ggagcggtag cggcagcggc ccttctgatc gactacgtgt
      901 tgaccgtctc ggtgtcggtg gcagcggggg ttgccgccat tacgtcagcg gtccccggcg
      961 tcatcccttg gcggatcgaa gtcgccgtcg gattcgtgat catcctcacc gtggccaacc
     1021 tgcggggggt aaaggaggcg tcgaccctgt ttgcagctcc cacctatctg ttcgtcgcaa
     1081 cggtggcggt gatgctcgtg gcgggatttg cgcaatgtct cgacggagaa tgccccaagg
     1141 cgatctcgtc gggaaccgag gtggaagctg aggttgccgt ggtcggactc ttcctgatcc
     1201 tgcgggcatt cgcatcgggt tccaccgcgc taactggggt cgaggcagtc gccaacggcg
     1261 tgcaggcatt ccgtcagccg aaggcacaca acgccgcaac cactctcggg atcatgggtg
     1321 cgatatcgat cacgatgttc ctcgggatct ccactctcgc tctctggttt gacgtgcgga
     1381 tctctgagca gacaatcgac gaatacggga ccgtgatctc tcagatcggc cgcgccgcgt
     1441 tcaacggcgg gattgggttc tacgtgctcc aggtgttcac cgcggccatc ctgatcctgg
     1501 cggcgaacac cgcctaccag gacttccctc gcctttcggc gatactcgcc cgccacaagc
     1561 tgatgccacg tcaatacctg aaccgtggcg atcgtctcgt attttccaac gggatcatca
     1621 ctctggcgct gatcgccgtg gccttgctca tcgtcttcga cgccgaggtc agccggctga
     1681 tccagctcta cgtcgtcggt gtgttcaccg cattcacgct cagtcagatc gggatggtgc
     1741 gacattggct caaggagcgg gacggaggct ggagacggtc ggttctcatc aacacggtcg
     1801 gcgctctgac cacgggagtc gttctcgtcg tcgtcgccac tgtcaagttc gcccacgggg
     1861 cctggatcgt ggtcctagcg atcccgatct ttgtcgtcgt catgttgagt atccgtcggc
     1921 actatcgggg ggtggccgcc caactcaggc tgatcccggt ggacgcggag ccccgtccga
     1981 cgaggatttt ggtgctgatt gctcaccacg atgaaggcac cgatcgagcg cttcagtatg
     2041 ccgagatggt cagggcagat gccatcacgt gtgtgcacgc agaggagcca tcgtcggacg
     2101 acctcgtcta cacctgggat gcagcgcatc cggtgcatgc actcgagatc atctccggag
     2161 agaaggactc catctcccga cggatcgtga atcgggtacg aaaggagcgt gaccttcacc
     2221 ccggagcgat gatcacggtg atcctcgccg atcgggttgc gactcgctcg atcctcgcgc
     2281 ccttcgctca ccggcacagc cttggcatca agtctcggct gcttttcgag cgcgatgtcg
     2341 tggtgaccga cctcaatgtg ctgagacgat ctcgacatag gcgcctcacc ccggtgccgg
     2401 tgagcagagt cgagcaagtg gtcctcgtgt cggacatgac ccgtccgatc agggaggccc
     2461 tcacctatgc ccagcgtttg ggaccatcgg ttacagcgct gcacatcgac gtcgattcgg
     2521 cacagcgcga ccggctggag aggcagtggg ccgaggctgg ctatgaattt cccctcgtgg
     2581 tcattcccag tgattttcgg agcatcgtcg acccgctcgt ccaatacctc cggcaacaga
     2641 ggagggcagc gagcccagga accctgatct cggcggttat ccccgagttc gtcgtgcccg
     2701 gtcgcataac ccaactgctc cacaatcaga ccgggctggc catcaaaggc attctcgccg
     2761 gagagtcggg catcgcggtc accagtgtcc ccttccatct ccaaccaaac gggcacggct
     2821 cgcgacctca gtagaggcca gcaccaatgc ctcattcagt cgtctcattg ccttgaatcc
     2881 acgagcagta acttgacatg gtgagccgcg gcaagctcaa ggtgttcctg ggggccgcac
     2941 cgggggttgg gaagacctac gccatgctgg acgaaggtcg ggacctccaa tcggcgggca
     3001 gagacgtggt cattggcttt gtcgaggctc acggacgtgg ggatacgatc gccatgagcg
     3061 cggacctgga ggttgtcccc ccaaggtgga tcgttgccga atcggaggcg tcggtcgagg
     3121 agatggatgt cgaagctctc ctcgctcggt gcccggaggt ggcgctggta gatgaactgg
     3181 cccacaccaa cgcttttggg tcgaagtatc ccaagagata tcaggatgtc gatgtcctcc
     3241 tcgaggccgg tatcgacgtt ctgaccacgg tgaacatcga gcatctcgag tccctcaatg
     3301 acgtcatctt cgagatcacc gctgtcgccc agccagacac gattcccgac ggagtcgttc
     3361 ggcgggccga tcagatcgaa ctcgtcgacc tgactcaaga gggagtcagg gagaggctgg
     3421 ccgcgggcaa gatcttcccc gctgagcaga tcgacgccac gctggcgaac tatttccgac
     3481 ccggcaacct gggggccctt cgggagctgg cgttgtcatg gaccgcagag cgagtagacg
     3541 aagcggtgac aacgtatcgg accgtccatg gcatcgatcg tccctgggag acgcgagagc
     3601 gcgttgtcgt agcgctcgcc ggcaccgacg gtggcgaagc cgtcgtcaga cgtgcggccc
     3661 gtctcgccat gcgatcccgg gcagacttgg tgggtgtcca cgttcgccca acccgcggag
     3721 tgatccaggg atcggacata gccttgacaa gccagcggga cctcctccag cgattggggg
     3781 gccgatacca cgaagtcgtc ggcgacgacg tcgggtcgtc gctgctcgca ttcgccaagg
     3841 aggaaaacgc cacccagatc gtgcttggtg cctctcgccg ctcccgagtc gacgagttca
     3901 ttcggagctc gccggttgct cgagtgatcc gcgacgccag cgatgtggac gtccatgtca
     3961 tctcgcatga aggtatgcga aggcaaagat cctcgttccg cttgccgaag aagtctccta
     4021 tcggctggtc acggagggca accgcgtggg cagttgcggt ccttggccta ccgcttctga
     4081 cgtggctttt gctgcgccgc ggagagcgtc ttgagttgca gaatgtgctg ctcgtatacc
     4141 tcctggtggc ggtcgtgacc ggggcgatcg gaggggcgct tcccgcggtc gttgccgcca
     4201 ttggcggttt cttcctcgcc aactggttct tcaccacgcc ggtggagacc ttcaccatcg
     4261 ccaacccgga tcagttgttg tcgctgtttg cattcctgtt cgtggccgcg acggtgggtc
     4321 tcctcgtcgg ctactccagc cggcggagcg cggaggcaag aacggcgagg gcgcaggcag
     4381 aggcactggc cgccaccacc acgctggccc accctgtctt cgccaacgac tacgaggcac
     4441 tgctgaggag cgttcgggat gcgctctctc tcgatgccgt gagtctggtt cgccgttcgg
     4501 cggacggttg ggagacgctg gcttgggagg gcgccgttga attgaagacc ccgccagacg
     4561 gcagcgagtc gatcgagctg gccggcgata tcgtgtttgt gcttcgcgat ggccggttga
     4621 cgagcgatga tcggatcgtt ctgcgcgctt tcgtcgccca aatggtccag gccatcgagc
     4681 gcgaagagtt gttacgcgag gcacgggcgg ccgaggggat ggccgagacc gatcggctgc
     4741 ggaccgctct cctcagcgca gtctcacacg atctccggac tccactggcc acgataaagg
     4801 cctcgttgac cagcctcatc gagaccggag tggagtggac gccggaacag acactcgagt
     4861 ttctcaacac agccctcgag gaaacggagc gcctcaaccg cctcgtgggc cacctcctcg
     4921 acgcaagtcg gctccaagcc aacgcggtgc acgtcttgtt caggccggtc gggctcgacg
     4981 aggtggtggc ttcggcgttg gctgggctgc gttcgaagac ggatcgggtg accgttgaca
     5041 tctcagagtc gctgccagag gtcaacaccg atcccgactt gatggagcgc gtgatcgcca
     5101 acctgatcga gaatgctgtg acatggtcgc cacccgacga gatggtccgg attactgccg
     5161 gtgaggtcca agaacgaatc gacctccgga tcatggaccg gggaccaggc atccccgctg
     5221 cctcgagtga cacgatcttc caaccgtttc agcgcacggg agactctcct gaccgagatg
     5281 gtgttgggct gggtctggca gtcgcaaaag ggctcctgga ggcgatggga aacgacctgg
     5341 tcatcgagga cacgcccggt ggcggtacca cgatggtgat cgccctggcg gcggccagtg
     5401 cgttggacgg ggtcgcacat gaaggcgcca cgatcggtgg ctagcaaaca gcgaatactc
     5461 gtggtggatg acgaggccca gatcttgcgc gcactgggca tcaacctcaa ggctcttgga
     5521 tatcaggtgg acaccgccgc ctcaggcgag gaagccctcc gaaaggccgc agaccatcgc
     5581 cccgacgccg tcatcctcga tctgggcctt cccggtatcg atggcatcga agtcatccgt
     5641 gggatacgag gctggagcag cgttcccatc atcgtgttgt cggtgcgaga cgcggaacgt
     5701 gagaagatcg ccgccctgga cgccggggcc gatgactacg tcaccaagcc gttcggcatg
     5761 ggcgagcttt tagcgcgact gcgagcggcc ttgcgccgag aagatggtca ggaaggcgag
     5821 ctgccggtgg tgacgaccga tgacttctcg atcgacttgt cgaccaagag ggttacccgc
     5881 gcaacgggcg acgaaatcaa gctcaccccc accgaatggg gcatagtcga gatgctggtt
     5941 cgccatcagg gaaagctggt gtcccagtcc caactcctca aggaggtctg gggaccccaa
     6001 tacggggacg aaaccaacta tctgcgcgtc ttcatggctc aacttcgaag gaaactggag
     6061 ccacaaccaa gccttccccg ctacttcatc acggagcccg gcatgggtta caggttcgag
     6121 ctagcggagg aagttggagc ttcggactga gctcggggtg cggctccgaa ttgagagcgc
     6181 atggcaaaga ttcagacggc cgaacgcgcc gggggcggag gaaccggctt gacgtgggcg
     6241 aagaggctgc gtgcgttcca ggtggggaat gcgcgcacga gcgacgaagg gccgtggagt
     6301 tggatgcggt cgtcgcgagt ggcctc
//
LOCUS       DASTXO010000081         2097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_925741, whole genome shotgun sequence.
ACCESSION   DASTXO010000081 DASTXO010000000
VERSION     DASTXO010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925741"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..>2097)
                     /locus_tag="VFU96_01610"
     CDS             complement(166..>2097)
                     /locus_tag="VFU96_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5111980.1"
                     /translation="GSYRYVSRQQGLSINFERVDNHWKGEKPAFKELEFRLAREESTR
                     LALVLSGEAHIADLPRELQKDALKKGLKIFSSSFPVDWMTVYFGGQYYMPGDPAFKPD
                     TPWAKKKVRQALNVAVNRKELLNTVFAGKGTMTYVSGWSAQSEGYNPEWQSRFDQMYG
                     YNPAKARALLKEAGYGPGQLKFKILAFTEPGESEGPQVAEALGIYYKDVGVETEIEVL
                     DWAKVREMFRKKTIQCCIWPNIISWRPSEEWVRTSYYSKSATHHYENEFIDKTYLELT
                     NAVKPADRQRLARAIGDHLFEEFADIPMFWFANEVVANPKVVGDRPRQTVPLLGIYGL
                     GQGVVGLVDELVLVVVVLALAPVADPDPLLRRAPGDDVGPDAALDRLLAKHLSYLGPV
                     EHLDLGVDADVPVVDAQRLGHLRPLRLPGFGERQDLELELSGAVAGLLQERLGLRGVE
                     AVHLVEPALPLRVIALRLSRPAGHVRHGPLAGEHRVEELLPVDGHIQGLPHRLLGPGH
                     VGLERRVPGHEVHSAQIHGHPVHGERRREDLHALLERVLLELAGQVGDVGLAGEDQGQ
                     PRRLLAGEPELQLLERRLLTLPVVVHALEVDGQALLATDVAIGAGPGRPLIEPFDPFL
                     VPLSLADHPEIAGAGHRIR"
BASE COUNT          395 a          660 c          667 g          375 t
ORIGIN      
        1 aacggccacg gccagttcac cgccaaggac gtcgtgcatt cacactctct catgatgcgt
       61 caggaggcga ccgcgacgct ggtccagacc tggcgcggcg tcgaggaggt gaaggtcgtc
      121 aacgaccacc aggtcgtgtt ccgcatgaag cggccgtcga ccaccctacc gtatgcggtg
      181 tcccgcgccg gcgatctccg gatggtcagc aaggctcagt gggacaagga agggatcgaa
      241 gggttcgata agcggccggc cgggaccggc tcctatcgct acatcagtcg ccagcagggc
      301 ctgtccatca acttcgagcg cgtggacaac cactggaagg gtgagaagcc ggcgttcaag
      361 gagctggagt tccggctcgc ccgcgaggag tcgacgcggc tggccctgat cctctccggc
      421 gaggcccaca tcgccgacct gccccgcgag ctccagaagg acgcgctcaa gaagggcatg
      481 aagatcttct cgtcgtcgtt ccccgtggac tggatgaccg tgtatctggg cggaatgtac
      541 ttcatgcccg gggacccggc gttcaagccc gacgtggcct ggaccaagaa gccggtgcgg
      601 caggccctga atgtggccat caaccggaag gagctcctca acacggtgtt cgccggcaag
      661 gggaccatga cgtacgtgac cggctggtcg gctcagtcgg agggctataa cccggagtgg
      721 cagagccggt tcgaccagat gtacggcttc aaccccgcga aggccaaggc gctcctgaag
      781 gaggccggct acggccccgg acagctcaag ttcaagatcc tggcgttcac cgaacccggg
      841 gagtctgagg ggcctcaggt ggccgaggcg ctgggcatct actacaggga catcggcgtc
      901 gacaccgaga tcgaggtgct cgactgggcc aaggtacgag agatgtttcg caagaagacg
      961 atccagtgct gcatctggcc caacatcatc tcctggcgcc cgtcggagga gtggatccgg
     1021 atcagctact ggagcaagag ccagaaccac cactacgaga acgagttcat cgacaagacc
     1081 tacaacgccc tgacccagac catagatccc aaggagcgga accgtctgtc gcgggcgatc
     1141 gccgaccacc ttcgggttgg cgacgacttc gttcgcgaac cagaacatgg gaatgtcggc
     1201 gaactcctcg aagagatgat cgccgatcgc ccgggccagg cgctgccggt cagccggctt
     1261 cacggcgttg gtgagctcca ggtaggtctt gtcgatgaac tcgttctcgt agtggtgcgt
     1321 ggcggacttg ctgtagtagc tggtgcgaac ccattcttct gacggccgcc acgagatgat
     1381 gttgggccag atgcagcact ggatcgtctt cttgcggaac atctcccgaa ccttggccca
     1441 gtcgagcacc tcgatctcgg tctcgacgcc gacgtccttg tagtagatac ccagtgcctc
     1501 ggccacctgc gggccttccg attcccccgg ctcggtgaac gccaggatct tgaacttcag
     1561 ctggccggga ccgtagccgg cctccttcag gagtgcgcgg gccttcgcgg ggttgtagcc
     1621 atacatctgg tcgaagcggc tctgccactc cgggttgtag ccttcagact gagcagacca
     1681 gccgctcacg taggtcatgg tccccttgcc ggcgaacacc gtgttgagga gctccttccg
     1741 gttgacggcc acgttcagag cctgccgaac cttcttcttg gcccatggtg tgtccggctt
     1801 gaaggccggg tcgccgggca tgtagtactg cccgccgaag tacaccgtca tccagtccac
     1861 ggggaacgac gacgagaaga tcttcagtcc tttcttgagc gcgtccttct gcagctcacg
     1921 gggcaggtcg gcgatgtggg cctcgccgga gagcaccaaa gccagccgcg tcgactcctc
     1981 gcgggccagc cggaactcca gctccttgaa cgcgggcttc tcgcccttcc agtggttgtc
     2041 cacccgctcg aagttgatgg acaggccctg ctggcggctg acgtagcgat acgagcc
//
LOCUS       DASTXO010000082         7000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_78286, whole genome shotgun sequence.
ACCESSION   DASTXO010000082 DASTXO010000000
VERSION     DASTXO010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78286"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VFU96_01615"
     CDS             <1..655
                     /locus_tag="VFU96_01615"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU5111981.1"
                     /translation="DKLAERHAQGFSWFKIKLGMAARDAELATVIHAADLVGEDGVVC
                     GDANEAWGEEEARSFLDLLDPDRVRFIEQPVPRAERDALIRLAETSPVRLCADESAGS
                     LDAVLGFVGTPLGGVSLKLIKHGGMTGVMRGAVICSAAGLDVNLAGKVIESSVSAAAN
                     LHCAAAMDRVDFGCSPANQGVVQDVTESPITVSGGHFAVPTAPGLGVDVEVEMLRHL"
     gene            complement(652..1491)
                     /locus_tag="VFU96_01620"
     CDS             complement(652..1491)
                     /locus_tag="VFU96_01620"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111982.1"
                     /translation="MTVYESPAKLNLNLLVEAPRADGLHFLQSLVQTIDWLDQLEVEE
                     AEEDILVIEGADLDSEDNLVTRSLKEIRARGFVPPLAITLDKRIPIGAGLGGGSSNAA
                     AMLLAAVETGRLPRAVAGEVAPSIGADVPLFLIGGTMLMTGIGDQVESLRPSSGFAVA
                     VAVPPFELSTTEVYLRWDEMEGPEGEVIAPRLLPPGLRDGMPIRNDLIPAAMTIEPAL
                     ADFMADLRKLWGSPVLMTGSGSACFGFFPDDEEAVDAGRSVEHLCRAVVGAGLRSNGV
                     APR"
     gene            complement(1488..2291)
                     /gene="rsmA"
                     /locus_tag="VFU96_01625"
     CDS             complement(1488..2291)
                     /gene="rsmA"
                     /locus_tag="VFU96_01625"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEU5111983.1"
                     /translation="MTAQTRSEIADLLSRHGLSPSRRLGQHFLADANITRKIVDTAGL
                     GPGLQVLEIGAGTGTLTRALAATGAHVVAYEIDAGLLPVLEEATRGLSVELRHLDVTR
                     ADLAGALGGGRWVMVANLPYNVGTPLVLDGLRRVPQIERFVVMVQREVAERLVAVPGN
                     KQYGLPSVVAGIHAVGRIAFTVPAQVFYPPPRVESAVVVLDRVAAPKDAERAIEIAGA
                     GFNQRRKMLRGALAGVFDDPATALVRTGIDPTARAEDLSPADYLALARA"
     gene            complement(2288..3034)
                     /locus_tag="VFU96_01630"
     CDS             complement(2288..3034)
                     /locus_tag="VFU96_01630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU5111984.1"
                     /translation="MQWVDTHCHLQLDERDPTLLLERAAEVDWVVVPGVDLESSRQAA
                     RMAAAHPGRVLATVGLHPHDAASWVEQSAGLTEMAPGAAAIGETGLDFYRNLSSRADQ
                     IRVFRRHVALALELEKPIVVHCRDAFAEVIEVIEDTGVGPQTVMHCWTAGPKWTRRYL
                     DLGVMFSFAGPVAFETGDTVRLGAAQVPPDRALVETDTPYLAPPPHRGQPNEPAWVAL
                     VGAALARVWGLDTEEVASVTSDNAARVFAR"
     gene            3103..3339
                     /locus_tag="VFU96_01635"
     CDS             3103..3339
                     /locus_tag="VFU96_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEU5111985.1"
                     /translation="METGIARKIDDLGRIVIPAETRRLFNIREGDHLFIAVEGGSIVL
                     RKLTDTCTFCGSETKVTQFKEKGICATCRAQLRS"
     gene            complement(3324..4163)
                     /gene="rsmI"
                     /locus_tag="VFU96_01640"
     CDS             complement(3324..4163)
                     /gene="rsmI"
                     /locus_tag="VFU96_01640"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU5111986.1"
                     /translation="MPEGGRLIVCATPIGNLGDVSDRLREVLQTADLVYAEDTRRTAT
                     LLRHVGASPTVRSMFVGNEKRRSGELLDSLDQGLTVALVTDAGMPTVSDPGSEVVRLA
                     RQAGHLVTVVPGPSAVVASVALAGFGGDRFVFEGFLPRKGKDRSLRLGRIGDEDRPVV
                     LFVSPHRLVADLESIAGATGPDREIVITREMTKVHEEVWSGSVGQAIEEWSNREVKGE
                     LTVVLAPGPAEPQSIDSAVAEARGLVESGTSPSEAARRVATATGASRREIYQVLIGDQ
                     ERS"
     gene            complement(4156..4473)
                     /locus_tag="VFU96_01645"
     CDS             complement(4156..4473)
                     /locus_tag="VFU96_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111987.1"
                     /translation="MQRVALTASEWGTSGYLAQTFTERLLGVRRFPGGSAVVLSAASV
                     HSFGLRRTLDLIGVDDRMRVVATMPLPPNRVRVIPSARLIVELPAGGPLPEIGEEIEI
                     TYA"
     gene            complement(4515..5831)
                     /locus_tag="VFU96_01650"
     CDS             complement(4515..5831)
                     /locus_tag="VFU96_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111988.1"
                     /translation="MTRRLASILAFALILGACTSTTADTTTTAPETSSTEAGGNTTTS
                     GGEASTTTTLAEPTWEELPGIDALPAAVQEELLGLVRTTEEIRGLRFQEPPTIVIVTE
                     QELEDRVRQQIEDESEDFPADEALYKLLGLLDAEVNLQSLLTELYGEQVAGYYDGETG
                     ELVVPMREEGFSVVQRGTMIHELTHALTDDQLDFHTSYTAMLDEDRLDEASAYQALIE
                     GDASMAELLYLQTLSQEELGEFFSEALDVDTAALDSAPQFIRDSLIFPYDSGLAWVQA
                     HYLQDQWTTVNEAYAEMPDLPGSTEQIMTPSDYARDLPRTFEATPPSVPGYELERESV
                     WGEFGFRIMIDQVLGEDVGVDAADGWGGDYYAQWFDGQNAALLILFEGDTDRDTEELR
                     SALLDYALSAVAEEDYVWVEVLDGRLAFIAADQPEVGESILASVSG"
     gene            5892..>7000
                     /locus_tag="VFU96_01655"
     CDS             5892..>7000
                     /locus_tag="VFU96_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:6165..6167,aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU5111989.1"
                     /translation="MGTLAIDGGKEVIVKRSYARLTQPLVRDNGSLRPASMEEAVERV
                     ATGLAPHMGDAFGLFSCSKGTNEMNYMAQKFSRVVMASNNIDSCNRTUHAPSVAGLAT
                     VFGAGGGTSSYREMEDTDLIVMWGSNAREAHPIFFHHVLKAINNGTRMFAVDPRRTSS
                     AAWADVWLGIDVGSDIALSNTVAWEIINSDLYHEDFVAHATSSFDEYVESVEHWTLKR
                     GQEVTGVPADAIKQLAHEYATAPTAQLCWTLGITEHHNATDNVLALINLALLCGHVGR
                     YGSGVVPIRGQNNVQGGGDMGALPNRLPGFQLVADPDLRGKFEKTWGCTIPHRPGKHI
                     SLMHEAMDDGELRALYVIGENPLQSEADAQQMERRL"
BASE COUNT         1277 a         2312 c         2260 g         1151 t
ORIGIN      
        1 cgacaagctg gcggagcgtc atgcccaggg cttctcgtgg ttcaagatca agctgggcat
       61 ggcagcccgc gacgccgagt tggccaccgt gatccatgct gccgatctcg tgggtgagga
      121 tggagtcgtc tgcggcgacg ccaacgaggc gtggggtgag gaggaggcga ggtcattcct
      181 cgatctcttg gatcccgacc gggttcgatt catcgagcag ccggtgccga gggccgagcg
      241 cgatgcgctg atccgcctgg ccgaaacgtc accggtcagg ctgtgcgcgg acgagagcgc
      301 tggaagcctg gacgcggtgc tcgggttcgt aggcacccct ttgggcgggg tcagcctcaa
      361 gctgatcaaa cacggcggga tgacgggggt gatgagaggt gccgtcatct gctcggcggc
      421 agggctcgac gtaaatctgg ccgggaaggt catcgagtcg agcgtctctg cggctgcgaa
      481 cctgcattgc gccgcggcaa tggaccgggt cgacttcggg tgcagcccgg ccaaccaggg
      541 cgttgtccag gacgtcacgg aatcgcctat caccgtgagc ggcggccatt tcgctgttcc
      601 caccgccccc ggcctcggcg tcgacgtcga agtcgagatg ctgcgccatc tctagcgcgg
      661 agccacgccg ttggagcgaa gaccggcacc gaccacagca cgacagaggt gctcgaccga
      721 gcggcctgcg tcgactgcct cttcgtcatc ggggaagaat ccgaagcaag ccgagccgga
      781 acccgtcatc agcaccgggc taccccacag tttccggagg tcggccatga aatcagccaa
      841 tgccggctcg atagtcatcg cggccgggat caggtcattg cggatcggca tcccatcacg
      901 gagccccggg gggaggagac ggggcgcgat cacctccccc tccggtccct ccatctcgtc
      961 ccagcgcagg tagacctccg tggtcgacag ctcgaaggga ggcaccgcta ccgccaccgc
     1021 gaatcccgac gagggtctca gcgattccac ttgatccccg atcccggtca tcaacatcgt
     1081 ccccccgatg aggaacagtg gcacgtcggc accgatcgag ggcgccactt ccccggccac
     1141 ggcgcgcggg agccttcccg tttcaacagc cgccagcagc atggccgctg cgttcgagct
     1201 gcccccaccg agcccggccc cgatcggaat ccgcttgtcg agcgtgatgg cgagcggtgg
     1261 gacaaagcct cgagctcgga tttccttcaa cgacctggtc accagattgt cctcggagtc
     1321 gagatcggcg ccttcgatga cgaggatgtc ctcctcggcc tcttcgacct cgagttgatc
     1381 gagccagtcg atggtctgga ccaacgactg cagaaaatgg aggccgtccg cccgtggcgc
     1441 ttcgacgagg aggttgagat tgagcttggc cggcgactcg tagacggtca tgctcgtgcc
     1501 agggccaggt agtccgccgg ggagagatcc tcagcccgag cggtcgggtc gatcccggtt
     1561 cggaccagag ccgtcgccgg gtcgtcgaaa acgccggcga gggcgccacg aagcatcttc
     1621 cgtctctgat tgaaccctgc gccggcgatt tcgatggccc gctcggcatc tttcggcgcc
     1681 gccacccgat ccagcacgac gacggccgac tcgacccggg gcggcgggta gaacacttga
     1741 gcgggaaccg tgaaggcaat ccggccgacg gcgtggatgc ccgccactac tgaaggcagc
     1801 ccgtactgct tgttgccggg cacggcgacg agccgctcgg ccacctctct ctgcaccatc
     1861 acgacgaacc gctcgatctg gggaacgcgg cgcaagccat cgaggacgag gggggttccc
     1921 acgttatagg ggagattggc caccatcacc cagcggccac cgcccaacgc gccggccagg
     1981 tcggcgcggg tgacatcgag gtgtcgaagc tcgaccgaca gcccacgtgt ggcctcctcg
     2041 agcacgggca ggagcccggc gtcgatctca taagcaacga cgtgggcgcc ggtggccgcc
     2101 agtgctcggg tcagtgtccc cgtgccggct ccgatctcga gcacttgtag tcccggcccg
     2161 agtccggcgg tatccacgat cttccgggtg atgttggcgt cggcgaggaa atgctgcccc
     2221 agccgtcgag acggcgagag cccgtgacgc gagagcagat ctgcgatctc ggacctcgtc
     2281 tgggcggtca tcgagcgaag actcgagccg cattgtctga ggtgacgctg gccacctcct
     2341 cggtgtcgag cccccaaacc cgggccaatg ccgcgccaac cagagccacc cacgccggct
     2401 cgtttggctg gccgcgatgt ggaggtgggg ccaaataagg ggtatcggtc tcgaccaacg
     2461 cgcgatcggg tggcacctgg gctgccccca ggcgaaccgt gtcccccgtt tcgaatgcga
     2521 ccggccccgc aaaagagaac atcaccccga gatccaggta gcgacgggtc cacttggggc
     2581 cggccgtcca gcagtgcatg accgtctgcg gcccgacacc cgtgtcctcg atcacttcga
     2641 tgacctcggc gaaggcgtcc cggcaatgga ccacgatcgg cttctccaat tcgagggcga
     2701 gcgcgacatg acgacggaac acccggatct ggtccgcccg cgaagagaga ttgcgataga
     2761 agtcgagccc tgtctcaccg attgccgccg cgccgggggc catctcggtg agcccggcgc
     2821 tctgctcgac ccaactggcc gcgtcgtggg gatgcaggcc gaccgtggcc agcactcgac
     2881 ccgggtgagc tgcagccata cgcgccgcct gcctcgagct ctcgagatcg acgccaggaa
     2941 ccactaccca gtcgacttca gcagcgcgtt ccaggagcaa cgtcggatca cgttcatcga
     3001 gttgaaggtg acagtgggtg tcgacccatt gcatgcaggg acggtacccg gcaccggttg
     3061 gaacaccccg ggtaggctcg cctcggggaa gctgaggtgg ttatggaaac aggcatcgcc
     3121 cggaagatcg acgacctggg ccggatcgtg atcccggcgg agacaagaag gctgttcaac
     3181 atcagggaag gggaccacct cttcatcgcg gtggaaggtg gctcgatcgt cttgcgcaaa
     3241 ctcaccgaca cctgcacctt ctgcgggtcg gagaccaaag tcacccagtt caaggaaaag
     3301 gggatctgcg ccacctgtcg ggctcagctc cgttcctgat ccccgatcag gacctgatag
     3361 atctcacgac gagacgcacc cgtcgcggtg gccacccgcc gggcggcctc ggacggagag
     3421 gtgccactct cgacgagacc cctcgcctcc gccaccgccg agtcgattga ttgcggctct
     3481 gcggggccgg gagcgaggac cacggtgagc tcacccttca cctcccgatt agaccattct
     3541 tcgatggcct ggccgaccga tccggaccag acctcctcgt ggacctttgt catctctctc
     3601 gtgatgacga tctcgcggtc aggtccggtc gcccccgcga tcgactcgag gtccgccacc
     3661 agtcgatgag ggctgacgaa caggacaaca ggccgatcct cgtcaccgat ccggcccaga
     3721 cgcagcgatc ggtctttgcc cttgcgcgga aggaagcctt cgaagacgaa gcggtcgccc
     3781 ccgaagccgg ctagcgcgac cgaggcgacc acggccgagg gccccggcac gaccgtcacc
     3841 agatgacccg cttgccgggc aagtcgcacg acttcggaac cggggtcgga caccgtcggc
     3901 atgccggcat cggtcaccag cgccacggtg agaccctgat cgagggagtc gaggagttcg
     3961 ccgctgcgcc tcttctcatt gcccacgaac atcgagcgca cggttgggct ggccccgaca
     4021 tgacgaagca gcgttgctgt ccgcctggtg tcttcggcgt agaccaggtc ggcagtctgc
     4081 agaacctcac gcagacggtc cgagacatcg ccgagattgc cgatcggcgt ggcgcagacg
     4141 atcaaacggc cgccttcagg catacgtgat ctcgatctcc tccccgatct cggggagagg
     4201 ccctccggca ggaagctcga cgatcaaccg ggccgatggg atcacccgaa cccgatttgg
     4261 gggcaacggc attgtcgcaa ccaccctcat ccggtcatcg actccgatca ggtccagggt
     4321 gcggcggagc ccgaagctgt ggacactggc ggcggacagg accacggccg agccgcccgg
     4381 aaagcgtcgc acaccgagca ggcgttcagt gaacgtttgg gccagatacc cgctggtgcc
     4441 ccattcggag gcggtcagcg cgactctctg catgcagacg acgctaggtg atcgtccgga
     4501 ccggggtaag tccttcagcc gctgaccgag gccagaatcg actcgccgac ctcgggctgg
     4561 tcggcggcga tgaatgccag acgcccatcg aggacctcga cccagacgta gtcctcttcc
     4621 gccaccgcgg agagggcgta atcgagcagc gcggacctca gctcttcggt gtcgcgatca
     4681 gtgtccccct cgaacaggat gagcaaggct gcgttttggc cgtcgaacca ctgggcgtag
     4741 tagtcgcctc cccaaccatc ggcggcgtcg accccgacgt cctccccgag gacctgatcg
     4801 atcatgatcc gaaacccgaa ctcgccccac accgactccc gctcgagctc gtaaccgggg
     4861 accgacggag gggtggcctc gaaggtcctc ggcaggtctc tcgcgtagtc acttggcgtc
     4921 atgatctgct ccgtcgaccc gggcagatcg ggcatctcgg cataggcctc gttcaccgtc
     4981 gtccattggt cttggagata gtgggcctgg acccaggcca gtccggagtc gtacgggaag
     5041 atcagtgagt cccggatgaa ttgcggagcg gaatcgagcg ctgccgtgtc cacgtccagg
     5101 gcctctgaga agaactcgcc gagctcttcc tgggagaggg tttgcaggta gaggagctcg
     5161 gccatgctcg cgtcgccctc gatcagggcc tgataggccg aggcctcgtc gagtcggtcc
     5221 tcgtcgagca tcgcggtgta gctggtgtgg aaatcgagct gatcatcggt cagggcatgg
     5281 gtgagctcgt ggatcatggt cccccgctgg acgacgctga agccctcctc gcgcatgggg
     5341 accaccagct caccggtttc tccgtcgtag taaccggcaa cctgctcccc atatagctct
     5401 gtcaggagag actgaagatt gacctcggca tcgagcagcc cgaggagctt gtacaacgcc
     5461 tcatcggccg ggaaatcctc ggattcgtcc tctatctgct gacgaacccg atcctcgagc
     5521 tcctgctccg tgacgatgac gatggtcggc ggctcctgaa agcgaagccc gcggatctct
     5581 tcggtggtgc ggaccagtcc gagcagctct tcctggacgg ccgcgggaag agcgtcgatt
     5641 ccggggagct cctcccaggt cggctccgcc agggtggtcg tcgtcgaagc ctccccgcca
     5701 ctcgtggtgg tgttgccccc ggcctcggta gacgacgtct ccggcgccgt ggtggtcgtg
     5761 tcggcagtcg tcgatgtgca agctccgaga atcagggcga acgcgaggat cgaggcaagg
     5821 cgtcgggtca cgatcgtcag gttatcggcc ccaaatcgct agatcgtgag tggcatccaa
     5881 cgcacccacg gttgggtacc ctcgccatcg atggtggaaa ggaggtgatt gtgaagcgaa
     5941 gctacgcaag gctgacccag ccgctggtgc gggacaacgg atcgctgcgc ccggccagca
     6001 tggaggaggc cgtcgagcgc gtggccaccg ggctcgcccc ccatatgggc gatgccttcg
     6061 ggctgttcag ctgctcgaag gggaccaacg agatgaacta catggcacag aagttctccc
     6121 gtgtcgtcat ggccagcaac aacatcgaca gctgcaaccg cacttgacac gccccctctg
     6181 tcgccggtct ggcgactgtg ttcggggcgg gtggcggcac tagctcctat cgagagatgg
     6241 aagacacaga cctcatcgtc atgtggggat cgaacgcgcg cgaggcgcac ccgatcttct
     6301 tccaccacgt cctgaaggcc atcaacaatg gcaccaggat gttcgcggtc gacccgagac
     6361 gaacctcctc ggcagcctgg gccgacgtct ggctcggcat cgacgtcggg tcggacatcg
     6421 ccctttccaa cacggtcgcc tgggagatca tcaattcgga tctctaccac gaggatttcg
     6481 tggctcacgc cacctccagc ttcgacgagt acgtcgaatc ggtggagcac tggacattga
     6541 aacggggtca ggaagtcacc ggcgtcccgg ccgacgccat caagcaactg gcccacgaat
     6601 acgccaccgc ccccaccgcc cagctctgct ggaccctggg gatcaccgag catcacaacg
     6661 ccaccgacaa cgtcctcgcc ctgatcaacc tcgctttgct ctgcgggcat gtcggccgtt
     6721 acggctccgg ggtggtcccg atccgcggcc agaacaacgt gcagggaggt ggcgacatgg
     6781 gcgccctccc caaccgcctg ccggggttcc agttggtggc cgatcccgat ctgaggggga
     6841 agttcgagaa gacctggggc tgcacgatcc cgcataggcc cggcaagcac atcagcctca
     6901 tgcacgaggc gatggacgat ggcgagttgc gcgccctcta cgtcatcggt gagaacccgc
     6961 tgcaatccga ggccgacgcc caacagatgg agcggaggct
//
LOCUS       DASTXO010000083         2007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1699401, whole genome shotgun sequence.
ACCESSION   DASTXO010000083 DASTXO010000000
VERSION     DASTXO010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1699401"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="VFU96_01660"
     CDS             <1..1087
                     /locus_tag="VFU96_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU5111990.1"
                     /translation="LARILFAETDVLLLDEPTNHLDLDAKAWLIEWLTTYRGGMLVVS
                     HDLPLLDEAITSVLALEHGELEAYRGNYSHYLVERDRRRVQKERERKHQDEAIARLEE
                     NIRRFKGTTEKMAKIARSWETRVDRMKRDLVEVQKGGKQVRVAFPQPEKSGRVPLSAM
                     GLAKSFGDNLVFVDVELAVERGERMLLIGLNGAGKTTLLRILAGVETADLGDVSLGHN
                     ASVGYYAQEHEQIQAGRSVLDHMRQDGSLTDQTLRSLLGHFLLADKVDQDGSTLSGGE
                     KTKLALAMLVAARPNVLLLDEPTNNLDPQAKEALLDALTIYEGTVVLVSHDTDFVAQL
                     MPDRAILMPEGEMRFFDEELLDLVSLA"
     gene            1131..1628
                     /locus_tag="VFU96_01665"
     CDS             1131..1628
                     /locus_tag="VFU96_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111991.1"
                     /translation="MRRHLLILALVAVAVACGSPDGIGSGGDPAPTTTIDPDQPVDSD
                     GDEPIAEPGPVGSIPEPRPPIDGSVDGEVWVTGADVRIMESFPVQIMLDVTGDKPTPC
                     HEIFWTVEDDGEVIAIEMISQVATEQNCAQVIEPFTIAVPLGSWTSESREIRLNGEVV
                     GSFET"
     gene            1898..>2007
                     /gene="paaA"
                     /locus_tag="VFU96_01670"
     CDS             1898..>2007
                     /gene="paaA"
                     /locus_tag="VFU96_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977797.1"
                     /note="with PaaBCDE catalyzes the hydroxylation of
                     phenylacetyl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HEU5111992.1"
                     /translation="MTSEERFEEIVGAEDKIEPADWMPEDYRKTLIRQIA"
BASE COUNT          381 a          642 c          637 g          347 t
ORIGIN      
        1 actggccaga atcctctttg ccgagaccga tgtcctcctg ctcgacgagc cgaccaacca
       61 cctcgacctc gacgccaagg cctggctgat cgagtggctc accacctatc ggggtggaat
      121 gctggtcgtg tcccacgacc ttcccctcct cgacgaggcg atcacctcgg tcctcgccct
      181 ggagcacggc gagctagagg cctaccgggg caattacagc cactacctgg tcgaacgaga
      241 tcgccgccgg gttcagaaag agcgcgagcg caagcatcag gacgaggcca ttgcccgtct
      301 cgaggagaac atccggcgct tcaagggcac caccgagaaa atggcgaaga tcgcccgatc
      361 gtgggagacc cgcgtcgatc gcatgaagcg agacctggtg gaggtccaga agggcgggaa
      421 gcaggtgcga gttgcctttc ctcagccgga gaagtcaggt cgtgtgcccc tctccgccat
      481 gggcctggcc aagtcattcg gtgacaatct cgttttcgtc gatgtcgagt tggccgtgga
      541 gcgtggggag cggatgctgc tgatcgggct caacggcgcg ggcaagacga ccctgctccg
      601 catcctggcc ggagtcgaga ccgcggacct cggcgacgtg tccttggggc acaacgcaag
      661 cgtcgggtat tacgcccagg agcacgagca gatccaggcc gggcgatcgg tgctcgacca
      721 catgcgccag gacgggagtc tgaccgacca gacgctgcgg tcgctgctcg gtcatttcct
      781 cctcgccgac aaggtcgacc aggacggctc gacgctctcc ggaggggaga agaccaagct
      841 ggcattggcc atgttggttg cggcacgtcc caatgtgctc ctgctcgacg agccgaccaa
      901 caacctggac ccacaggcga aggaggcact gctcgatgcc ctcaccatct acgaggggac
      961 tgtggtgctg gtgagccatg acacggactt cgtggcccag ctcatgcccg atcgggcgat
     1021 cctgatgccg gaaggcgaga tgaggttctt cgatgaagaa ctgctcgacc tcgtctccct
     1081 ggcctgatgg ccggcgggaa ccatcggccg cgtcagggca tccaagagtc atgagaaggc
     1141 atctcttgat cctcgccctg gtcgccgttg ctgtcgcctg tggctcgccc gatgggatcg
     1201 gatccggggg agacccggca ccaacgacga cgatcgaccc cgatcagccg gttgactccg
     1261 acggagacga gccgatcgcc gagcccgggc ctgtcggatc catccccgag ccgcggccgc
     1321 cgatcgatgg gagcgtggat ggggaggtgt gggtgaccgg cgccgacgtg cgaatcatgg
     1381 agagcttccc ggttcagatc atgctcgacg tgaccggcga caagccgact ccatgccacg
     1441 agatcttctg gacggtcgaa gacgacggtg aggtgatcgc gatcgagatg atctcccagg
     1501 tggccacgga acagaactgc gcccaggtga tcgagccttt caccatcgcg gtgccactcg
     1561 gcagctggac ttcggagagc cgggagatcc ggctcaacgg tgaggtcgtc ggctccttcg
     1621 agacctgagc tggaaacggt gaagggaggg cctccctgtg gtgaggccct cccttcgggt
     1681 gatttcaggg accgttaccg ggcactttcg actgcaagcc aaatgccgaa ggggtgagcc
     1741 cccagcctcg atccccgctt ccttcgcgct ctctcccagc gcactgacca atttccctct
     1801 tttccgccgc cgactccaga cccgttcccg attcaccccc ggtgactagt tcatctgacc
     1861 tcgacaaacg aacgttcgtt tgtgttatcc tgaatccatg acctccgagg agcggttcga
     1921 ggaaatcgtc ggcgccgagg acaagatcga gcccgccgac tggatgccgg aggactaccg
     1981 gaagaccctg atccgccaga tcgccca
//
LOCUS       DASTXO010000084         3444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_78342, whole genome shotgun sequence.
ACCESSION   DASTXO010000084 DASTXO010000000
VERSION     DASTXO010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78342"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="VFU96_01675"
     CDS             <1..1032
                     /locus_tag="VFU96_01675"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5111993.1"
                     /translation="VRDTAYVPDTHVQMYEELKSEVVAFGHSTGSPHTMAINPSLQAD
                     GILAIPLTWYSGWSDPAINANLVPHGTPYCIEAMNMIEYLTLQDPEAATIAIATNGGD
                     YGLDSSAGAVMVAEALGLEVVYDGSGVIVATDPATLAEAATGIAGSGADLVWVTTDPT
                     SFGGIYGQALAAGFSGALWSGAGPTWNPALVAPDSPIAEPLANDFYVSAYYAPWAAES
                     PGNQMVRDLVEAAGAPPLDYYAEGVIEATIMHQALLAASEAGDMTQAGVLAAAKSLES
                     VTFDGLAPDETYVGEPNDRLQRKQFISRPDPAGLAGGTSTGVTLVESEYTSPAAEAFE
                     FTGACFAIE"
     gene            1101..1892
                     /locus_tag="VFU96_01680"
     CDS             1101..1892
                     /locus_tag="VFU96_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5111994.1"
                     /translation="MLAVENLEVVYNDVVLVLRGISLQVPDGKIVALLGTNGAGKTTT
                     LRAITGLLDMHEGDVTKGDITFDGIDITDEDPAARVRRGISQVMEGRRVFAEMTVDDN
                     LRAGSYTNRDAVRVKEARERVFALFPKLADRRNATAGYMSGGEQQMLAIGRALMQDPK
                     LLILDEPSLGLAPRLVQEVRDIIVEINSQGTSVLLIEQNAKMALSIADYGYIMETGKV
                     VMDGDPAKLLKDEDVKEFYLGLHTTGEQKSFRDVKHYKRRKRWLS"
     gene            1880..2722
                     /locus_tag="VFU96_01685"
     CDS             1880..2722
                     /locus_tag="VFU96_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5111995.1"
                     /translation="MALVSGKLLERRAERSGPKAYDHLLEVMDVHLAFKGVKAVDGVS
                     FHVDDGELFAIIGPNGAGKTSLFNVVNQVYSPQSGDVRWKGESLMGVKPNETAERGIA
                     RTFQNIALFPHMTVIENLLTGRHVRMRSSWLAGSIWLGKAKREEIENREIVEDIIDFL
                     EIEQWRKYPVGLLPYGIQKRVELGRALAMDPELLMLDEPVAGMNLEETEDMARFILDV
                     QDELGIAIILVEHDMGLVMDIADRILVVDFGKPIAEGTPSSVQHNPNVVKAYLGEEVA
                     PLDS"
     gene            complement(2732..3313)
                     /locus_tag="VFU96_01690"
     CDS             complement(2732..3313)
                     /locus_tag="VFU96_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEU5111996.1"
                     /translation="MTSAVIAQRVLPRSRITSVLLVVGAAALTALAAQWEIHLPFTPV
                     PVTGQTFAVLLTGAALGMTLGAAGQAVYVVAGALGLPVYAGGESGWSTATTQGSAGYL
                     IGFIFAAGVVGFMAERRQDRTFPTMFTAFIAGSFIIYAFGVAGLMILLDLTFTEAVLA
                     GVVPFILGDIIKASAAGLLLPGAWRLVGEYPRP"
BASE COUNT          658 a         1056 c         1140 g          590 t
ORIGIN      
        1 gttcgtgaca ccgcgtacgt ccccgacaca catgtgcaga tgtacgagga gctcaagtcc
       61 gaggtcgtcg cgttcggaca ttcgacaggg tcgccccaca ccatggcgat caacccgtcg
      121 ttgcaggccg atgggatcct cgccatccct ctgacctggt actcgggttg gtccgaccca
      181 gccatcaacg ccaacctcgt gcctcacggg acgccgtact gcattgaggc gatgaacatg
      241 atcgagtact tgactttgca ggatcccgag gcggcgacca tcgcgatcgc aacgaacggt
      301 ggtgactacg ggctggattc gagcgcaggc gccgtgatgg ttgctgaagc cctcggcctc
      361 gaggtggtct acgacggcag cggcgtgatc gtggccaccg atccggccac gcttgccgag
      421 gcggcaaccg ggattgccgg atcgggagca gatctggtct gggtcacgac cgatccgaca
      481 tcctttggcg ggatctacgg gcaggccctg gccgccggtt tctccggcgc tctgtggtcc
      541 ggcgccggac ccacctggaa ccctgccctg gtcgccccgg actcgccgat cgccgagcca
      601 ctggccaacg acttctatgt ctcggcgtac tacgcgccat gggctgcgga gtcgccgggc
      661 aaccagatgg ttcgcgatct cgtggaggcg gccggtgcgc cccccctcga ctactacgcc
      721 gagggcgtaa tcgaggcgac gatcatgcat caggccttgc tcgcagcttc tgaggcgggt
      781 gacatgaccc aggccggtgt cctggccgcg gcaaagagtc tggagtcggt gaccttcgac
      841 ggtttggcgc cggacgagac ctacgtcggc gagccgaacg accggttgca gcggaagcag
      901 ttcatctcgc ggcccgatcc tgccggtctg gccgggggaa cgtcgaccgg ggtcaccctg
      961 gtcgagtcgg agtacacgtc tccggctgcc gaggcattcg agttcacagg agcctgcttc
     1021 gccatcgagt agcgctgaca tatccgcatg gtgggcggtc ttcggaccgc ccaccacgtg
     1081 agcatgagga gaacagagac gtgttagccg tcgagaacct cgaggtggtc tacaacgacg
     1141 tcgtcttggt gctccgcggc atctcgctcc aggtcccgga cggcaagatc gtggctctgc
     1201 tcgggaccaa cggtgcgggc aagaccacca cgcttcgtgc gatcaccggg ctcctcgaca
     1261 tgcacgaggg tgacgtgacc aagggggaca tcaccttcga cgggatcgac atcacggacg
     1321 aggacccggc cgctcgggtt cggcggggga tcagccaggt gatggagggt cgacgcgtct
     1381 tcgccgagat gaccgtcgac gacaatctga gggccggttc ctataccaat cgggacgcag
     1441 tccgcgtgaa agaggcgcgt gagcgggtgt ttgccctctt ccccaagctg gccgaccggc
     1501 gcaacgccac cgcaggctat atgtcgggtg gtgagcagca gatgctggcg atcggtcgcg
     1561 ccctgatgca ggatccgaag ctgctcatcc tcgacgagcc ctccctcggc ctcgctcccc
     1621 gtctggtgca ggaagtccgg gacatcatcg tcgagatcaa ctcgcagggg acatcggtgt
     1681 tgctcatcga gcagaacgcc aagatggccc tttccatcgc cgactacggc tacatcatgg
     1741 agaccgggaa ggtggtcatg gatggcgacc cggccaagtt gctcaaggac gaggacgtca
     1801 aggagttcta cctcggcctg cacaccaccg gtgagcagaa gtccttccgc gatgtgaagc
     1861 attacaagcg ccgaaagcga tggctctcgt gagcggaaag ctcctcgaac gccgggcgga
     1921 gcgatccggg ccgaaggcat acgaccacct tctcgaggtg atggacgtgc acctggcgtt
     1981 caaaggggtc aaggcggtag atggggtttc tttccatgtc gatgacggcg agctcttcgc
     2041 catcatcggg cccaacggcg cgggcaagac atcgctgttc aacgtcgtca accaggtgta
     2101 cagcccgcag tcgggggatg tccggtggaa gggcgagagc ctgatggggg tcaagccaaa
     2161 cgagaccgcc gagcgcggca tcgctcgcac ctttcagaac atcgctctct ttccccacat
     2221 gaccgtgatc gagaatctgt tgaccgggcg tcacgttcgc atgcggagct cgtggctggc
     2281 aggaagcatc tggctgggga aggcgaaacg ggaggagatc gagaaccgcg agatcgtgga
     2341 ggacatcatc gatttcctcg agatcgagca gtggcggaag tacccggttg gcttgctccc
     2401 ttatgggata cagaagcgcg ttgagctggg ccgcgcgctg gccatggatc ccgagctcct
     2461 gatgctggac gagccagttg ccggtatgaa tctggaggag accgaggaca tggcgcggtt
     2521 catcctcgac gtccaggacg aactcggaat cgccatcatc ctggtcgagc acgacatggg
     2581 cctggtgatg gatatcgccg accgcattct cgtggtcgat ttcggcaagc cgatcgccga
     2641 aggcaccccc tcgtccgtcc aacacaatcc caatgtggtc aaggcctacc tcggcgagga
     2701 ggtggctccc ctggattcct gaggaacgcg gtcagggccg cgggtattca cccaccagcc
     2761 gccaggcgcc agggagaagg aggccggcgg ctgaggcttt gatgatgtca ccgaggatga
     2821 aggggacgac gccggcgagg acggcctccg taaaggtcaa gtcgaggagg atcatcagcc
     2881 cggccactcc gaatgcgtag atgatgaacg agccggcgat gaaagcggtg aacatggtgg
     2941 ggaaagttcg gtcctgacga cgctcggcca tgaagccgac cacaccggcg gcgaagatga
     3001 acccgatcag atagccggcg ctgccctgcg tggtcgcggt gctccagccg gattccccgc
     3061 ctgcgtacac cggtagtccc aacgcaccgg cgaccacgta gacggcttgg ccggcggccc
     3121 ccagggtcat gcccagggcg gcgccggtga ggagcacggc gaaggtctgt ccggtcaccg
     3181 ggacaggggt gaatggcagg tggatctccc actgcgcggc cagggcggtc aatgcagcgg
     3241 ctccgaccac gaggagaacc gatgtgatgc gtgagcgggg aaggacgcgt tgggcaatga
     3301 ctgccgatgt catatttatc tcctacaaac ccgacgtgtg tcaccttaaa gacctcggac
     3361 cggcgctcgc ggcccaccgt taccctttca tgatggaaga ccaaaaccaa cgccgatctt
     3421 cctggcccct ctacctgatc gggg
//
LOCUS       DASTXO010000085         2656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1109994, whole genome shotgun sequence.
ACCESSION   DASTXO010000085 DASTXO010000000
VERSION     DASTXO010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109994"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1369
                     /locus_tag="VFU96_01695"
     CDS             <1..1369
                     /locus_tag="VFU96_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019631280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEU5111997.1"
                     /translation="SSRQWYIRNGGREQELRSQFLKRGSELDWIPDFMQVRYEHWVNG
                     LTGDWLISRQRFFGVAIPVWYPVGDDGTIDHEHPILPDESDLPVDPTSAAAPGYEEAQ
                     RGQPGGFVGDPDIMDTWATSSLTPQVAGHWDDDPDLFSRVFPMDLRPQGPEIIRTWLF
                     STIVRSHYEHDALPWAYTTINGWVLDPDRKKMSKSKGNVITPHALIEEHGAEAVRYWS
                     CNGRPGTDTAVDHGVMRIGRRLATKILNASRFALGFAEDVDPSAISASLDRSMLATLA
                     QVVDGATEALDAYDYARALEIVERSFWTWTDDYLELVKGRAYEGGNEGASAHAALQLG
                     LGVYLRLFAPFLPFVTEEVWSWWREGSVHRASWPSIEELVVYSGDPAVLSETGAVLSA
                     VRKAKSDAKVSMRAEVDSVSVSAGRASIELVRQAEDDLKAAARAETVVYSEGEFAVES
                     VLSAN"
     gene            1371..2105
                     /locus_tag="VFU96_01700"
     CDS             1371..2105
                     /locus_tag="VFU96_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family zinc transporter"
                     /protein_id="HEU5111998.1"
                     /translation="MVEAFLWGLLAASSLLIGALIAVGRPPHRRMLGLIMAFGAGVLL
                     SAVSFELIAEATDVGGGSGAVVTGIFAGAAAFTIGDILIGRFQRRRQGSVMPDSEEGA
                     GLSILLGALLDGVPETAVLGLTLLQTGEVGVAMLVAVFISNLPEGVAATTTLIEGGWK
                     RSLVLEIWGAVVLACALAAAAGYVLLDGASTGTISFVFAFSGGAVLTMLATSLIPEAY
                     EHAGRAVGVVTVFGFILAYGIHLLEG"
     gene            complement(2110..2274)
                     /locus_tag="VFU96_01705"
     CDS             complement(2110..2274)
                     /locus_tag="VFU96_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5111999.1"
                     /translation="MPVHRGKDSKGPFYQWGDSGKKYHYQGGEKKSREAAKQKATKQG
                     QAARASGYKG"
     gene            complement(2330..>2656)
                     /locus_tag="VFU96_01710"
     CDS             complement(2330..>2656)
                     /locus_tag="VFU96_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5112000.1"
                     /translation="GNYLASLQKLLGLNPGITYPAHGTLIDRGDERARQILLHHDRRL
                     LDMAELVRSRDSTAWEVMLQSFRPNLTPLEARLAFLETVSHLEHLRMTGRLQTEQRDG
                     TLVFRR"
BASE COUNT          413 a          804 c          894 g          545 t
ORIGIN      
        1 ctccagccgg cagtggtaca tccgcaacgg tgggcgggaa caggagctgc gatcacaatt
       61 cctgaaacgc ggctcggagc tcgattggat accggatttc atgcaggttc gctacgagca
      121 ctgggtcaat gggttgaccg gcgactggct tatctcgcgg caacgcttct tcggtgtggc
      181 catccccgtc tggtatccgg tcggtgacga cgggacgatc gaccacgagc atccgatcct
      241 tcccgacgag agcgacctgc ctgtcgaccc gacctcggcg gctgcaccgg gatatgaaga
      301 ggctcagcgg ggccagcccg gtggcttcgt gggtgatccg gacatcatgg acacctgggc
      361 cacctcttcg ctgacaccgc aggtcgccgg gcactgggac gacgaccccg atctcttctc
      421 ccgggttttc ccgatggatc tgagacccca gggcccggag atcatccgca cctggctctt
      481 ctccacgatc gtccgctcgc actacgagca cgatgcgttg ccctgggcct acaccacgat
      541 caacggctgg gtattggacc cggaccgaaa gaagatgtcc aagtccaagg gaaatgtgat
      601 cacgcctcac gccctgatcg aagagcacgg tgccgaggcg gtccgctact ggtcttgcaa
      661 cggtcgtccc gggaccgaca ccgcggtcga ccatggtgtc atgaggatcg ggcgccgtct
      721 cgcgacgaag atcctgaacg cgtcccgctt cgctcttggg ttcgccgagg acgtagatcc
      781 atctgccatt tcggcctcac tcgaccggtc gatgctggcc accctggccc aagtggtgga
      841 cggggcgacc gaggcattag atgcatacga ctatgcccgt gccctggaga tcgtcgagcg
      901 gtcgttctgg acctggacgg acgactacct ggagttggtc aaaggcaggg catacgaggg
      961 cgggaacgag ggtgcatcgg ctcatgccgc gctccagttg gggctcggcg tgtatttgcg
     1021 gctctttgcc cctttcctcc cattcgtgac tgaggaggtc tggtcctggt ggagagaggg
     1081 gtcggttcac cgggcaagct ggccaagcat cgaggagctt gtcgtctact ccggggatcc
     1141 tgcggtcctg tccgagacgg gtgcggttct ctccgcggtg cgcaaggcga aatccgatgc
     1201 caaggtctcg atgcgggccg aggtggactc ggtctcggtc tcggccggcc gagcgtcgat
     1261 cgagctggtg agacaggccg aagatgatct gaaggcggcg gcccgggcgg agacggtggt
     1321 ctactccgag ggtgagttcg cggtcgaatc ggtgctgagc gccaactgac gtggtcgagg
     1381 cgtttctctg gggcctgtta gccgccagct cactgctgat cggagccctc atcgcagtcg
     1441 gccgtccgcc tcaccgtcgc atgctgggtt tgatcatggc gttcggtgcg ggtgtgctgc
     1501 tcagcgcggt ctcgttcgag ctgattgcgg aggcgaccga tgttggcggt ggcagtggcg
     1561 cggtggtgac ggggatcttc gccggggctg ccgccttcac gattggcgac atcctcatcg
     1621 gacgattcca gcgacgacgt cagggcagtg tgatgccgga ctccgaagag ggcgccgggc
     1681 tctcgatcct cctcggggcg ctgctcgacg gtgtgccgga gacagccgtt ctcggcctga
     1741 cgctgcttca aaccggcgag gtgggggtgg cgatgctggt tgcagtcttc atctccaacc
     1801 tgcccgaagg agtcgccgca accaccaccc tgatcgaggg tggttggaaa cgctcgctgg
     1861 tcttggagat ctggggagca gtggtgttgg cttgtgctct ggccgcagcg gcgggatatg
     1921 tcctgctcga cggggcctcg accgggacca tctcgttcgt cttcgctttc tcgggtggcg
     1981 cggtgctgac gatgctcgcc acttcgctga ttcccgaggc gtatgagcac gccggacgtg
     2041 ccgtgggcgt cgtgaccgtg ttcgggttca tcctcgccta cggcatccac ctgctcgagg
     2101 gatgaccgct cagcccttgt agccggaggc gcgtgccgcc tgtccctgct tcgtggcctt
     2161 ctgcttggct gcctccctgc tcttcttctc cccaccctga tagtgatact tcttgcccga
     2221 gtccccccat tgatagaagg ggcccttgct gtctttgcct cgatgtaccg gcatggattc
     2281 ctcccacttg gtcgcaaggg attacccgcc gggcaatcgg gagaaactct cagcggcgga
     2341 atacgagtgt gccgtcgcgc tgctcggttt ggagtctccc tgtcattctg aggtgctcca
     2401 gatgggagac ggtctccaag aaggccagcc tcgcctccaa cggggtcagg ttgggccgga
     2461 aggattggag catcacctcc cacgccgtgg aatcgcggga tcggacgagc tcggccatgt
     2521 cgagaagacg gcggtcgtga tggagcagga tctgtcgtgc cctctcgtct ccccggtcga
     2581 tgagggttcc atgggcggga taggtgatcc cggggttcaa tccgagcagc ttctggagtg
     2641 aggcgaggta gttccc
//
LOCUS       DASTXO010000086         2137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_152177, whole genome shotgun sequence.
ACCESSION   DASTXO010000086 DASTXO010000000
VERSION     DASTXO010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152177"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="VFU96_01715"
     CDS             complement(<1..976)
                     /locus_tag="VFU96_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU5112001.1"
                     /translation="MTQLDPSPPKLRPTRRSWRDLPASPPIGDPHPPITQEQPMVAPV
                     APTEVVEKAPKSGVRIFTFALTTVVVVLVGAAVWLWQREAPVTTTPTVAANEAEAGSP
                     GAVAAALGPAVVHIEILGGLEAGGGVGSGVIYDTSGLVLTAHHVVSFSDEVTVKTADE
                     RSFDGRVVGRIPERDLAIVALTDATDLVAATIAEPGTVEVGEPAIALGSPFGFQQSVT
                     AGIISGLDRELETPMGTLTGLIQTDAPINPGNSGGPLADAEAKVIGINTAIASMSGGN
                     DGVGFAVPVEDFASLIDEVEANGGVDAPTVPAPEDAGALGGLVPGLEDL"
     gene            complement(1078..>2137)
                     /locus_tag="VFU96_01720"
     CDS             complement(1078..>2137)
                     /locus_tag="VFU96_01720"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEU5112002.1"
                     /translation="SGRVDTVMGWMRWLEEKRLVEHYPEVAVHGALIFALLGQPGACD
                     RWAAAAEVASPHANIPDGSTMDGYLAYLRANLCRNGNDEMRVDSLRALEGLSPASPYR
                     ATMTFTAGLSYLLEGDAERADAEFVPAFDATMHARQLPFAAVILAERCIAAAERDDWS
                     ETEELTSRAVSIVQEGGFDDYWTSAMVYAWAAREALHRGRITKGRDFVARAARLRSLL
                     TYALPVVSVQALIELARSYVALGDRDGAKTVLRQATDILRLRPSLGRLPDQVEQVRRM
                     VERIPVGNMGSSSLTGAELRLLPYLSTHLTFGEIADRLYVTHSTVKTQTTSIYRKLGV
                     SSRSAAIERLTETGLLGS"
BASE COUNT          384 a          736 c          642 g          375 t
ORIGIN      
        1 gaagatcttc gagaccaggc accaatcccc ctagtgcacc ggcatcctcc ggagcgggga
       61 cggtcggggc gtcaacgcca ccgttcgcct cgacctcgtc gatcagcgac gcgaagtcct
      121 cgactggcac cgcgaacccg acaccgtcgt ttccgccaga catcgaagcg atcgccgtgt
      181 tgatccctat caccttggcc tcggcgtcgg ccagtggccc tccggagtta cccgggttga
      241 tcggggcatc ggtctgaatg agcccggtga gagtgcccat aggcgtctcc agctctcgat
      301 cgagacccga gatgattccg gccgtcaccg attgctggaa tccgaacggg ctgcccaagg
      361 cgatcgccgg ttcgccaacc tcgaccgtcc ctggctctgc gatcgtcgcc gccaccagat
      421 cggtcgcatc cgtgagagca acgatggcga gatcacgctc tgggatccga cccaccacac
      481 gcccgtcgaa cgaacgctcg tctgcggtct tgacagtcac ctcatcggaa aaggagacaa
      541 cgtgatgtgc ggtgagcacc agcccggatg tgtcgtagat cacccctgag cccacgcctc
      601 cgccggcctc caacccaccg aggatctcga tatgaaccac ggccgggcct aatgcggcgg
      661 caactgctcc ggggcttccg gcttctgcct cgttggcagc aacggtcggg gttgtcgtga
      721 caggcgcctc acgctgccag agccagaccg cagcaccgac caagaccaca acgacggtgg
      781 tcagggcgaa cgtgaagatg cgaacaccgc tcttcggcgc cttttcgact acctccgtcg
      841 gcgccaccgg agcaaccatt ggctgctcct gagtgatcgg cgggtgcggg tcgccgattg
      901 gcggcgacgc cggcagatca cgccatgatc gccgtgtggg gcggagtttg ggcgggctcg
      961 gatcgagttg cgtcaaggct tcctccgtgt ggtcaaccgc cgcaacatat gggacgccct
     1021 ttaaaaggcc cgtcaagccc ggaccgtttc agaccaatcg gtggtccggt cgcttgatta
     1081 cgatccgagc agacccgtct cggtcaacct ttcgatggca gcgctcctcg aggagactcc
     1141 cagtttccgg taaatcgacg tggtttgggt cttcaccgtg ctgtgcgtga catagagccg
     1201 gtcggcgatc tccccaaagg tgaggtgggt cgacaggtac gggagcaatc gaagctctgc
     1261 gccagtcagg gacgacgatc ccatgttgcc gaccgggatc ctctcgacca tccggcgcac
     1321 ctgttccact tggtcgggaa gccggcccag cgagggtcgc agccgcagga tgtcggtagc
     1381 ttggcgaagg acggtcttcg ccccatccct gtcccctagc gccacgtagg accgcgccag
     1441 ctcgatcaac gcctgcaccg acaccacggg tagcgcatac gtgaggagac tcctcagccg
     1501 cgccgcccgg gcaacgaagt ccctcccctt agtgatcctt ccacgatgaa gtgcctccct
     1561 cgccgcccac gcatacacca tggcgctggt ccagtaatcg tcgaagccgc cctcctgcac
     1621 gatcgagacc gccctggacg tgagctcttc tgtttcggac caatcgtcgc gctcggcggc
     1681 cgcgatacac cgctcggcca ggatcaccgc cgcaaaagga agctggcggg catgcatcgt
     1741 tgcatcgaag gcggggacga actcggcgtc tgcccgctct gcgtcgccct ccagcaggta
     1801 ggacagtccc gcggtgaagg tcatggtcgc ccggtatggg cttgccggac tcaacccttc
     1861 caacgccctc agggaatcga ctcgcatctc atcgttgccg ttgcggcaca agttggccct
     1921 cagatacgcc agatagccat ccatcgtgct gccgtcggga atgttggcgt gcggggaggc
     1981 gacttcggct gccgctgccc agcgatcaca ggcccccggt tggccgagca gggcaaatat
     2041 caatgcaccg tgcacggcca cttccgggta gtgctccacg agccgcttct cctcgagcca
     2101 ccgcatccaa cccatcaccg tgtcgacccg gccgctc
//
LOCUS       DASTXO010000087         4178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1404966, whole genome shotgun sequence.
ACCESSION   DASTXO010000087 DASTXO010000000
VERSION     DASTXO010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404966"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VFU96_01725"
     CDS             <1..364
                     /locus_tag="VFU96_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112003.1"
                     /translation="ARLFGAFGATTETAVISAYTVHRSATTTGMFNEDTLSRLNGVFK
                     DVEGFGVLTPREVERCRSRHMHQFVLGGTYRSIRSHDNDSARRIMALLESPEFETVRF
                     SWKWLAVRLGFSIYLKLF"
     gene            complement(356..1606)
                     /locus_tag="VFU96_01730"
     CDS             complement(356..1606)
                     /locus_tag="VFU96_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112004.1"
                     /translation="MSSTVDQEQRLRHKLPAGIVDAALSSLGTFAAGLAAVRLLNPAE
                     LGIYSVIFSAFLVASLLPAEITYTPIQVSMIRLDAQERLGGIVHSLGKGLPPALLGCV
                     LILVAGGLIWWRSDHTAVIELAITGVGLTVISPIQDHLRRGLHIAEKSWDAALMSAAQ
                     LLAVVGALVIMQLLEVAPAWAPLGSLVVANTVSLGFGLLLSRPAWRLRPAAPVVTVSG
                     RWLAASSVTAYGGTLVASVVMTLVAGPEIVGFAEAARVAAQPVLVVTTGVAAALASRH
                     LRAADTVDKAAAMMVNKSFFLVVAIAGISYLAIAGFAWSFNPVFALIPSAYEIAWLIP
                     VTILSNLVLSFTSPLRDQLIASNKEVPMAKIDFASLAIPTVVAATAGVTGAFARPLGI
                     IGQNAFRTIRYRSIWRTAFAGGSE"
     gene            1856..3772
                     /gene="asnB"
                     /locus_tag="VFU96_01735"
     CDS             1856..3772
                     /gene="asnB"
                     /locus_tag="VFU96_01735"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEU5112005.1"
                     /translation="MCGIAGTYNLRIDPGVAEAVSMAIAHRGPDAAGYWNSDDPGLPV
                     WLCHRRLSIIDLSSAANQPFEKDGLILIFNGEIYNYRQLRSELSARGAGFRTSSDSEV
                     LLEAWRNWGPASLDRLRGMFAFALYDVDNRRLVLARDPFGIKPLFYQVRNGGLAFASE
                     LKALRPMMGEVRVDETALVASVMYSWVPETHCMFEGTQKLAPGTWLEIGPDGQHRVHR
                     YWDPVSAATAAYEAGQRDRFDPPRLGQVMEDSVAAHLVADVPISTFLSGGLDSSLITV
                     MAKKQNAEIDSYTISFRDEDQRYEAMPDDFSYAKRVAAEHGIRLHEVELAPDVADLLP
                     KLVHHLDEPIGDAAAINAYLICQAARDAGVKVLLSGMGADELFGGYRKHHAALLAARY
                     RHIPRWLRQRLIEPTIQHLPVASKKRGYRLSRWAHRFVDFASLPEEAAFRRSYTHYDQ
                     AQLADLLAPELSVRVEGLVDEHRDVYSSYQGEDQVNRMCFTDTQMFLPSLNLAYTDRA
                     SMAASTEVRVPFVDKHVFEAAFAIPGSDKIVGRERKAILKEASLPWLPKEIAYRPKGL
                     FSAPLRAWIRRDLQEMVEEYVGLGHLVGSGMLDKSAVRIMIDEDRSGMTDRSKEIWQL
                     LTLELWHRQVLNLE"
     gene            complement(3813..>4178)
                     /locus_tag="VFU96_01740"
     CDS             complement(3813..>4178)
                     /locus_tag="VFU96_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112006.1"
                     /translation="KTPRELLDLRPGELVRVKTKEEIAQTLDRRNRNRGLTFDSEMLR
                     YCGGEYRVLRRVSRMINEGTGELFELPGECIVLDTVTCTAEYHRLCRRSHYPYWREIW
                     LTRVTQPVEITRSSETRAG"
BASE COUNT          818 a         1283 c         1304 g          773 t
ORIGIN      
        1 cgcgagactg ttcggggcct tcggggccac gaccgaaacc gcggtcatct cggcctacac
       61 ggttcatcgc tcggccacta ccaccgggat gttcaacgag gacacgctgt ctcgtctcaa
      121 cggcgtgttc aaagatgtcg agggctttgg ggtcctgaca ccacgagagg tcgaacggtg
      181 ccggagtcgg catatgcatc aattcgtcct tggtggcacc tacagatcca tcaggtccca
      241 cgacaacgat tcggccagac ggatcatggc cctgctcgag tcgcccgagt tcgagacggt
      301 ccggttctcg tggaaatggc tcgccgtccg cctcgggttc tcgatctatc tcaaactatt
      361 ctgagccccc tgcgaaggcc gttctccaga tcgagcggta gcggatcgtc cgaaacgcgt
      421 tctgcccgat gattcccaac ggtcgggcaa aggcgccagt gacgccggcc gtggccgcga
      481 ctacggtggg gatcgccagc gaggcgaaat cgatcttcgc catcggcact tccttgtttg
      541 acgcgatcaa ctgatcgcga agtggagagg tgaagctgag cacgaggttg gagagaatgg
      601 tgaccggaat cagccaggca atctcgtagg cgctcggaat cagcgcgaac acggggttga
      661 agctccaggc gaacccggcg atggcgagat agctgattcc ggctatcgcc accaccaaga
      721 agaaggattt gttgaccatc attgccgcgg ccttgtccac cgtgtccgcc gcacggagat
      781 gtcgcgaggc gagcgcagct gcgaccccgg tggtgacaac caggaccggt tgggcggcca
      841 cccgagccgc ctcggcgaac ccgacgatct cgggtccggc caccagggtc atcacgaccg
      901 atgccaccag cgtcccgccg taggcggtga cggaagaagc ggccagccat cgcccggaga
      961 cggtcaccac cggggcagct ggccggaggc gccatgccgg cctcgacaac agcaggccaa
     1021 aacccagcga gaccgtgttt gccaccacga ggcttcccag cggcgcccag gccggcgcta
     1081 cctccaacaa ctgcatgatg acgagggcac cgacgactgc cagtagctgc gccgcggaca
     1141 tcagcgccgc gtcccaggac ttctcggcga tatggagacc tctgcggaga tggtcttgga
     1201 tcggtgagat gactgtcagg ccgactccgg taatggccag ctcgatcacc gcggtgtgat
     1261 cggatcgcca ccagatgaga cctccggcca ccaggatcaa gacacaaccg agcaaagccg
     1321 gggggagccc cttcccaagt gagtgaacga tgccgcccaa tcgctcttga gcgtcgagtc
     1381 tgatcatcga gacctgaatc ggggtgtagg tgatctcggc gggcagcaga ctggcaacca
     1441 gaaaggcgga gaagatcacc gagtagattc caagctcggc agggttgagc aacctgacag
     1501 ccgccagccc cgcggcgaac gtacccaggg aagacaaagc ggcgtcgacg atcccggctg
     1561 ggagcttgtg tcgtaggcgt tgctcttggt cgacagtcga ggacattaga aggcccaccc
     1621 aaatcaggca cgcgtccttc cctcgcgcgt gcgatgaagc gaatcgtttg gcccgaaggc
     1681 cggagaaccc tccccgggat cctggtgaga gcgtaactgg gtcagccagc ccatgtgata
     1741 tcgaagcgcc tctgcgtaga atcccatctc tcgaggatgg tcgtgccacg agcggccgat
     1801 cggctgcagg ccggttgggg gagtcgctga tggcgaaggg gaggcggact gttcaatgtg
     1861 cggcattgcc ggtacctaca acttgcggat tgatcccggg gtggcggaag ccgtgtcgat
     1921 ggcgatcgcg catcgcggac cggatgctgc cgggtattgg aacagtgacg acccgggcct
     1981 accggtgtgg ctgtgtcatc gacgactgtc gatcatcgac ctctccagcg cggccaatca
     2041 gccgttcgag aaggatggcc tgatcctcat cttcaacggt gagatctaca actaccgcca
     2101 gttgcgaagc gaactgtctg cccgaggggc cggatttcgc acctcgtccg actcagaggt
     2161 cttgctcgag gcctggcgta attggggacc ggccagcctg gatcgccttc gtggcatgtt
     2221 tgccttcgcc ctctacgacg tcgacaaccg cagactcgtc cttgcccgcg accctttcgg
     2281 cataaagcca ctcttctatc aggttcgaaa cggcgggctg gcgttcgcct cggagctgaa
     2341 ggctttgcga ccgatgatgg gcgaagtccg ggtcgacgag actgcgctgg tggcatcggt
     2401 catgtattcg tgggtacccg aaactcattg catgttcgag ggaacccaga aactcgcacc
     2461 ggggacctgg ttggagatcg gtcccgacgg tcagcaccgt gttcatcgat actgggatcc
     2521 tgtctcagca gccaccgcgg cctatgaggc cgggcaacga gacagattcg atccaccccg
     2581 tctgggtcag gtaatggagg attcggtagc tgcccacctg gtggcggacg tgccgatctc
     2641 gaccttcctc agcggtggtc tcgactcgag cctcatcacc gtcatggcca agaagcagaa
     2701 cgccgagatc gacagctaca cgatttcgtt ccgcgacgag gaccaacgat atgaggccat
     2761 gcctgacgac ttctcctacg ccaagagggt tgctgcggag catgggattc gcctccatga
     2821 ggtcgaactc gcacccgatg tggccgatct gctccccaag ttggtgcatc atctggatga
     2881 gccgataggc gatgccgccg cgataaacgc ctatctcatc tgtcaggcgg ctcgcgacgc
     2941 cggagtcaag gtcctgctgt ccggcatggg cgccgacgag ctgttcggcg gctatcggaa
     3001 gcatcatgcg gccttgcttg ccgcccggta tcggcacata ccccgatggc tgcgtcagag
     3061 gttgatcgag ccaacgattc agcatctccc ggtcgcgagc aagaagcggg gatatcgcct
     3121 gagtcgctgg gcacaccgtt ttgtcgattt cgccagtctc ccggaggagg cagcttttcg
     3181 aaggagctac acgcactacg accaggccca gcttgcagat ttgctcgctc cagaattgtc
     3241 agtccgtgtg gagggactgg tcgacgaaca ccgcgacgtg tactcgtcct atcagggtga
     3301 ggatcaggtc aatcggatgt gtttcacgga cacccagatg tttctcccga gcctgaacct
     3361 ggcctacaca gaccgggcca gcatggctgc ctcaaccgag gtgcgagtgc cgttcgtcga
     3421 caagcatgtc ttcgaggcgg cctttgccat ccccggctcc gacaagatcg ttggtcggga
     3481 acggaaggcg atcctcaagg aggcctcatt gccatggctg cccaaggaga tcgcctaccg
     3541 gccgaagggc cttttcagcg ccccactacg tgcttggatc cggcgtgatc tccaggaaat
     3601 ggtcgaggaa tacgtcgggc taggtcatct ggtcggttcg ggaatgctcg acaagtccgc
     3661 ggttcgcatc atgatcgacg aagaccgcag cgggatgacc gatcgctcca aggagatctg
     3721 gcagctgctc accctcgagc tctggcatcg ccaggtcctg aacctcgagt aactgaggcg
     3781 gacagaactg acgaccggtc gcggtcgccg gcctaccccg ccctcgtttc cgaggacctt
     3841 gtgatttcga ccggctgtgt caccctcgtc agccagatct cccgccagta cggatagtgg
     3901 ctgcgtcgac atagacgatg gtattccgca gtgcaggtga cggtgtcgag gacgatgcat
     3961 tcccccggta gctcgaacag ctcaccagtc ccctcgttga tcattcggct gacccggcgg
     4021 agcacgcggt actcaccacc gcagtagcgc agcatctcgg agtcgaaggt caaaccgcgg
     4081 ttgcggtttc ggcggtccaa cgtctgggca atctcctcct tcgtcttcac gcgaacgagt
     4141 tcaccggggc ggaggtcgag cagttccctc ggagtctt
//
LOCUS       DASTXO010000088         2985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_484098, whole genome shotgun sequence.
ACCESSION   DASTXO010000088 DASTXO010000000
VERSION     DASTXO010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484098"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VFU96_01745"
     CDS             <1..214
                     /locus_tag="VFU96_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5112007.1"
                     /translation="VAENAGGAISALAAAGIDARQDGGALLVGEDDGSLVVRVLAEAG
                     IYPSEVTPARSTLESVFLGMTGEESP"
     gene            211..975
                     /locus_tag="VFU96_01750"
     CDS             211..975
                     /locus_tag="VFU96_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112008.1"
                     /translation="MSLLHAEYLKLSRRRLYYMMVIILAALVGLLAFFLLIFAQIAPE
                     LAEGVPLLRKPVAYIFGAQQVAGQTWFPLILAVVMLGSEFGSTVWATALTRDPRRVGQ
                     ISARFVVLALASLLAIAAGIAGWATVTAIAVPGEGGPGVDEWLGLVWKVGLIELAWTA
                     IGLGAVAQLRSVGPAIGAALALYFVDPILGLWGPWETVSLSAASSALFQIDIPGGFGA
                     FVPGTDLSLSHAVAVMLGWTALGLFLTWWGLQRRDA"
     gene            complement(957..1172)
                     /locus_tag="VFU96_01755"
     CDS             complement(957..1172)
                     /locus_tag="VFU96_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112009.1"
                     /translation="MGDLLGVTVAPVQPYAARKEYICPGCQTIIPPGTFHLVVVPDDE
                     PDLRRHWHHGCWHKEMRRRFGAQASRR"
     gene            1309..2088
                     /locus_tag="VFU96_01760"
     CDS             1309..2088
                     /locus_tag="VFU96_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEU5112010.1"
                     /translation="MSWLHTEDRGPVSWLTIDRPDRKNAIPFDGWSDLRATLDAFEAS
                     SQRVLVVTGAGGDFCAGADLDPSRLDRLQSVEDRHLRMKEVGAAAMALHRLTKPAIAA
                     VDGFAVGAGMNLALGCDVVVATDRARFSEIFVKRGLTVDFGGSWLLPRIVGMQRAKEL
                     ALSGRLVEADEALRIGLVTEVVPVEALEQRVVEIAEGFLAGAPVAQMFAKQTLNASFQ
                     IGLADALSWEGEAQSVALGTEDAVEGLQAFLEKRDPEWTGR"
     gene            2169..>2985
                     /locus_tag="VFU96_01765"
     CDS             2169..>2985
                     /locus_tag="VFU96_01765"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEU5112011.1"
                     /translation="MERVYSNDQVEQVLGNLFSGLSVFHASICEWLIDADMSQQFLAD
                     GAKSLPEWVSARFGLRHSTAAQLVRVARRLEDLPVLRSRFAAGELSMDQVDAISKLAT
                     RHTEEAVISDCLGMSNAALDRAARRAEPPSTEDELDSWRDRWLSIQYTLDGIRGHMDA
                     DLPGSEMSLVESAIREKADRIPPNPESGLLDPYPQRMADGLVELCSTTGDEYGPSPTQ
                     IVVHADLDALTSESETGGAELEGGPVIANETARRLSCDPIIECTVYDHARTLGI"
BASE COUNT          486 a          886 c          998 g          615 t
ORIGIN      
        1 tgtcgccgag aacgcgggcg gggccatctc cgcgctggct gcagccggaa tcgacgcgag
       61 acaagacggt ggagcccttc tggtgggaga ggacgacggc agcctcgtgg tcagagtgct
      121 ggccgaagcc ggcatttatc ccagtgaggt cactccggcc cgatcgactc tcgaatcagt
      181 gttcctcggg atgaccggcg aggagtcgcc atgagcctgc ttcatgccga gtacctgaag
      241 ctgagccgac gccggctcta ctacatgatg gtgatcatcc tggccgcgct cgtcggcttg
      301 ctcgccttct tcctcctgat cttcgcccag atcgcccctg agctggccga aggtgtgccg
      361 cttctcagga aacccgttgc ctacatcttt ggcgcccagc aagtggcagg tcagacgtgg
      421 ttcccgctga tcctggccgt cgtgatgctg ggcagtgagt tcgggagcac ggtatgggcc
      481 acggcgctga cccgagatcc acgccgggtc ggtcagatct cggcccgatt cgtggtcctc
      541 gccctggcct cgctcctcgc gatcgccgcc ggcatcgccg ggtgggcgac ggtcaccgct
      601 atcgcagtgc caggggaagg aggaccgggc gtcgacgaat ggctgggcct ggtatggaag
      661 gtcggtctca tcgagctggc gtggaccgcg attggtctcg gggcggtggc tcagctccgg
      721 tcggtcgggc ctgcgatcgg agcggcgctc gccctgtact tcgtcgaccc gatcctcggc
      781 ctgtggggac cttgggagac cgtgtccctg tctgcggcca gcagcgccct cttccagatc
      841 gacatccctg gcgggttcgg cgcgttcgta ccggggaccg atctgtcgct atctcacgcg
      901 gtcgcggtga tgctgggctg gacggctctc ggtctgttcc tgacctggtg gggccttcag
      961 cggcgcgacg cctgagcgcc gaaccgtcgt ctcatttcct tgtgccagca cccgtgatgc
     1021 cagtgcctcc gcagatcggg ctcgtcatcg ggcaccacca ccagatggaa ggtgccgggg
     1081 ggaattatgg tctgacaacc gggacagatg tactccttgc gcgccgcgta gggctgcacc
     1141 ggggcgaccg tcaccccgag aagatcgccc atgccaggac cgcgaaaatc gccacgaacg
     1201 cgactaccag cgcgacgatg tcggcagtgc ttcgctcatg atcacgaaac ggattgaacc
     1261 ccatgggctc aacggtaccc acgccctgtg ttgtctaccg tcatcgtcgt gagctggttg
     1321 cacaccgaag atcgcgggcc ggtgagctgg ctcacgatcg atcgaccgga tcgaaagaac
     1381 gccatcccat tcgatggctg gtcagatctg cgagcgaccc tcgatgcatt cgaggcgtcg
     1441 tcgcaacgcg ttctggttgt caccggagcc ggtggggact tctgcgccgg cgccgatctc
     1501 gacccctctc gcctggatcg gctccagtcc gtggaggacc gtcacctgag gatgaaagag
     1561 gtgggcgccg cggcgatggc actgcatcgc ctgaccaagc ctgctatcgc cgcggtcgac
     1621 ggctttgctg tcggggctgg gatgaacctg gctctcggct gtgatgtggt cgtggctacc
     1681 gaccgagccc ggttctccga gatcttcgtg aagagggggc tcacggtcga cttcggtgga
     1741 tcgtggctgc tgccccggat cgttggcatg caacgggcga aagaacttgc gctgtcgggc
     1801 cgcctcgtgg aggccgacga ggcacttcgg atcggattgg tcaccgaagt cgtgcctgtc
     1861 gaggctctcg agcaacgtgt ggtcgaaatc gccgaaggct tcctcgccgg agcacccgtg
     1921 gcccagatgt tcgccaagca gaccttgaac gccagtttcc agatcggcct cgccgatgcc
     1981 ctttcgtggg agggtgaggc tcagtccgtg gccctcggca ccgaagatgc cgtcgaggga
     2041 ttgcaggcct ttctcgagaa gcgggacccg gaatggaccg gcaggtaggc gaggaagatt
     2101 ctcggatttc cttctgattg gctggtttct gaccggtttc tgtcacacct gtttcgtacg
     2161 gttgtggcat ggagagggtg tattcgaatg atcaggtcga gcaggtcttg ggcaaccttt
     2221 tctcggggct gtccgtgttt cacgcttcga tctgtgaatg gttgatcgat gctgatatga
     2281 gtcagcagtt cttggcggat ggggcgaaga gtcttccgga atgggtgtcg gcccggtttg
     2341 ggttgcggca ttcgaccgcg gcccaacttg tccgggtggc tcgacggctg gaggatctgc
     2401 cggtccttcg ttcccggttc gcggccggcg aattgagtat ggatcaggtc gatgccatct
     2461 cgaagctggc gacgcgtcat accgaggagg cggtgatctc cgactgtctc ggaatgtcga
     2521 atgctgcttt ggatcgggcc gcccgccggg cggagcctcc gtcgactgag gatgagctcg
     2581 attcctggcg ggatcgttgg ttgtcgatcc aatacacgtt ggatgggatc cgaggtcaca
     2641 tggatgcgga tctgcctggt tcagaaatgt cattggtgga atcggcgatc agggagaagg
     2701 cggatcggat cccacccaac ccggaatcgg gactgttaga cccgtatccg cagcggatgg
     2761 ccgacggact ggtcgaactg tgctctacca ctggtgatga gtacggaccg tctccgactc
     2821 agatcgtcgt ccatgccgat ctagacgcgt tgacctctga atcagaaacc ggaggagcgg
     2881 aactggaggg tggtccggtc atcgccaatg agaccgctcg gagactctcc tgcgatccca
     2941 tcatcgaatg caccgtctat gaccatgctc ggactcttgg gatcg
//
LOCUS       DASTXO010000089         2295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1626078, whole genome shotgun sequence.
ACCESSION   DASTXO010000089 DASTXO010000000
VERSION     DASTXO010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626078"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="VFU96_01770"
     CDS             <1..877
                     /locus_tag="VFU96_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEU5112012.1"
                     /translation="TKFVDSLLNDLEEAGVKDQPLGVDFIDINLIKKFEEREISWTDG
                     MTPMMEARAIKSPDEIKAFSIVGAICDYVHYEITNFIKPGLTENEVAAFGFEKLYSLP
                     GMEDVEDVIVSSGPNAWPNWRNFSDRMIRPGELVIVDLAALTWNGFKSCVYRTYCVGG
                     KPTDEHQKTYDLAHKWLWDSIEATKPGVTTADVASKWPSAKEVWGYEEEDQAAANFWG
                     HGLGLAQYDPPVISRIWSLDHPVEIQPGMTFALETQHGKLHDHGVRLEEMLYVTDTGI
                     EMVSSYASHEIISVI"
     gene            884..>2295
                     /locus_tag="VFU96_01775"
     CDS             884..>2295
                     /locus_tag="VFU96_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEU5112013.1"
                     /translation="MIHDLDHEAARDPARVGAKAAWLAMARRSGLPVLPGLVIDTGDS
                     LEHMRLGSEKLGTRGSGGARLAMTAEPISFGADLVEHGARLGEVLVARSSTIIEASGE
                     WAGAFTSYLDLSPADLPRAVTGCWASAFSVAALERQEAAGIEPGSFPMAVLVQPALDP
                     VAGGTASIEEDGSIVVIGVKGSPAALLQGWSAGLEASRHEEGPWVGKDLVDLLGLETL
                     DEIARALDLARSSLGANRCEWGLDGQVWLMQLSRSESPAHSPQPRSRPADRRLLPIAR
                     VVAKAAGPIGEELILPWALGGLPATGAPSHVLRDDAMGNARAMRDSLTAQVWRLPAED
                     ALAAARSCMTALLGPDPLSALEVVGNLRTPDPEQASSLWALVQSLQLQDPAHRLGVGR
                     WEPFLASVILDSGTRHQGTGAAPGVGAGISTGRYPPDGTDVAYRPRAVIIASQPIPNL
                     APLLWDAAGLVTESGSPAAHL"
BASE COUNT          388 a          762 c          753 g          392 t
ORIGIN      
        1 caccaagttc gtcgattccc tcctcaatga cctggaggag gcgggagtca aggaccagcc
       61 gctgggggtc gacttcatcg acatcaacct gatcaagaaa ttcgaggagc gcgagatcag
      121 ctggacggac gggatgaccc cgatgatgga ggcgcgcgcc atcaagagcc ccgatgagat
      181 caaggcattc tcgatcgtgg gggcgatctg cgactacgtc cattacgaga tcaccaattt
      241 catcaagccg gggctgaccg agaacgaggt ggcggccttc ggcttcgaga agctgtactc
      301 cctgcccggg atggaggacg tcgaggatgt aatcgtctcc tcaggcccca acgcctggcc
      361 caactggagg aacttctccg accggatgat ccgccccggg gagctggtga tcgtcgacct
      421 ggctgccctc acctggaacg gcttcaagtc gtgcgtgtat cgcacctatt gcgtcggggg
      481 aaagccgacc gacgagcatc agaagaccta tgacctggcc cacaagtggc tatgggactc
      541 gatcgaggcg acgaagccgg gggtcaccac cgccgatgtc gcctcgaagt ggccgtccgc
      601 caaagaggtg tgggggtatg aggaggagga ccaggctgcc gcgaacttct ggggccatgg
      661 tctcgggctc gcccagtacg acccaccggt catctcccgg atctggtcgc tggaccaccc
      721 ggtcgagatc cagccaggga tgaccttcgc cctcgagacc caacacggaa aactccatga
      781 tcacggcgtg cgtctcgaag aaatgctcta cgtgacagat acaggaatcg agatggtctc
      841 cagctacgcc tcacacgaga tcatctcggt catctgaccg ggtttgatcc acgacctcga
      901 tcacgaggcc gctcgcgacc cggcccgggt cggggccaag gccgcctggc tcgccatggc
      961 ccgccgctcc gggctcccgg tcctgccggg gctggtgatc gacaccggtg actcgctcga
     1021 acacatgcgg ctggggagcg agaagctggg cacccgtggc tcgggggggg cacggctggc
     1081 gatgaccgcc gaaccgatct cgttcggtgc cgatctggtc gagcacggtg cccggctggg
     1141 agaagtcctg gtcgcccgtt cgtcgacgat catcgaagcc tccggtgaat gggccggtgc
     1201 cttcacctcc tatctagacc tgtcccctgc ggatttgccc agagcagtca ccgggtgctg
     1261 ggcatcggcg ttctcggtgg cggcgctgga gcgtcaggag gcggcaggaa tcgagcccgg
     1321 gtccttcccc atggccgtgc ttgtccagcc cgccctggat ccggtggcgg ggggcaccgc
     1381 ttcgatcgag gaggatggct ccatcgtcgt gatcggtgtg aagggctcgc ccgccgcgct
     1441 gctccaagga tggagtgccg ggctggaagc gagccgacat gaggaagggc cctgggtcgg
     1501 caaagacctg gtcgacctgc tcggtctgga gacgctcgac gagatcgccc gcgccctcga
     1561 tctcgcccgc tcgtcattgg gcgccaaccg atgtgaatgg ggactcgacg gccaggtgtg
     1621 gctgatgcag ctctcccgga gcgaatctcc ggcgcactct ccgcagccgc ggtcgcgtcc
     1681 cgccgatcgc aggctcctgc cgattgcccg cgtcgtggca aaggcggcgg ggccgatcgg
     1741 cgaagagctc attcttccct gggccctcgg tggcctcccc gccactggag caccatcaca
     1801 tgtcctccgc gacgatgcca tgggcaacgc ccgcgccatg cgggacagcc ttaccgccca
     1861 ggtgtggaga ctgcctgccg aggacgcact agccgcggct cggtcttgca tgacggcgct
     1921 tctcgggcct gaccctctgt ctgcgctcga ggtcgtcggc aatcttcgca cgcccgatcc
     1981 agaacaggct tcgtcgcttt gggctctggt gcaaagtctg caactacagg acccggccca
     2041 ccgacttgga gtcggcaggt gggagccctt cctggcctcg gtcatcctcg actcggggac
     2101 caggcaccag ggcaccgggg ccgctcctgg cgtcggggcc gggatctcca cggggagata
     2161 tccccccgat gggaccgatg tcgcctaccg cccccgagcc gtgatcatcg cctcccagcc
     2221 cattcccaat ctggccccac ttctctggga cgccgccggg ttggtgaccg agtcgggcag
     2281 cccggccgcc catct
//
LOCUS       DASTXO010000090         3982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_926148, whole genome shotgun sequence.
ACCESSION   DASTXO010000090 DASTXO010000000
VERSION     DASTXO010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926148"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="VFU96_01780"
     CDS             complement(<1..803)
                     /locus_tag="VFU96_01780"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEU5112014.1"
                     /translation="MGTRTPTRKGSSGRRRVSTAKKKKTAPLSAIRQKLVAVRGSVRN
                     TLGRQTDDIWGMILVVLAILVLLAFVDLAGPVGEGMAGGSRFLFGVWRFALPVALAGI
                     GVAMIVGKPRDGAKRLIAGGIVTFLGSLALFHLMTGAVALAPSLELVEERGGAVGSLI
                     AFPMRRVLGMWGAFVVLASAMGMGVLIMTQTSVRELAAGIADLYRSTRRMASSAMVGA
                     RTQRPAHVSDRVAKPASDNRGRHERPKPARPKAEKPKEPATPKPKPVPLP"
     gene            complement(928..2562)
                     /locus_tag="VFU96_01785"
     CDS             complement(928..2562)
                     /locus_tag="VFU96_01785"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU5112015.1"
                     /translation="MSVSVTFLGGLGEIGRNCAAVEVEGRIALIDCGLMFPEEDMLGV
                     DLVFPDWSWLTERSGDVECVVLTHGHEDHTGALAYFLREVNVPVYGTKFTIELARGRI
                     DELGVEADLRQVQVKQWFDHTPFRFQLIPVSHSVPDATAVAVETPEGVILHSGDFKLD
                     PTPIDGVPTDLPAFATLGRRGVRLLLADSTNAERPGFEPSESSVGQPITDVIRDAGGR
                     VIAACFASHIHRVQQIADAGIRNGRKVAFFGRSMHRNTALAAELKELDVPPNSVMDIK
                     DLAELPANQQLLITTGSQGEPFAALSLMSQGQHKFVELGEGDTVLISAKPIPGNETAV
                     SKVISNLIRRGALVVHGTNAHVHVSGHANREELLTFLNLVRPQAFVPVHGEYRHLHAH
                     AELARKMGVRHVDVLEDGDRVILAGKKTQVQRRAVPAGYVYLDGSAVGDVQSAVLRDR
                     SHLADEGVVVVTVGIDHDTGEVLYGPDLDSHGLMDDPGTVLAKAANAVTEAIGEHASG
                     RSDIGELQKTIRQAVGRVIRAETARRPVIVPVVMEL"
     gene            complement(2559..3452)
                     /gene="dapA"
                     /locus_tag="VFU96_01790"
     CDS             complement(2559..3452)
                     /gene="dapA"
                     /locus_tag="VFU96_01790"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEU5112016.1"
                     /translation="MSSFPSAPFGHVATAMITPFAASGEVDHEKAWRLARFLSDNGSD
                     ALVVTGTTGESPTLSSVEKVALYKTVVDAVAGKDTFVIAGTGTYNTAESVELTAKASD
                     LGCAGILAVAPYYNKPDQAGLVSHFSAIADVGLPVMLYNIPGRTGSRIEIDTLARLAE
                     HESIVAVKDAVLDIEFTGHTINRVPGLTVYSGQDSFTWPMMAVGAVGVVSVVSHLAGR
                     AVSAMVRAALSGDVVEARRLHHLLLPLCEACFLETNPTPVKGAMDRLWEPVGDVRLPL
                     VKASEETLVAVEKAVGALQGL"
     gene            3491..>3982
                     /locus_tag="VFU96_01795"
     CDS             3491..>3982
                     /locus_tag="VFU96_01795"
                     /inference="COORDINATES: protein motif:HMM:NF020022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5112017.1"
                     /translation="MELRLPAPCLVVLVGASSSGKSTWAGRAFKETEVVSSDRLRGIV
                     GSGEDDQQAGTAAFSILEQIVEERMRRRLTTVIDTLGFDREKRRRWVALAHEADLPAF
                     AVVFDTPPEQARVRNAEQVRPIPKTVLDKQLARFRVVRTEIDEDGFDRVLVEQPVALV
                     APSM"
BASE COUNT          695 a         1285 c         1332 g          670 t
ORIGIN      
        1 gggagaggga ccggcttcgg cttgggcgtg gcgggttcct tgggcttctc ggccttggga
       61 cgagcgggct tcggccgctc atgccgaccc cggttgtcgg acgccggttt ggcgacccgg
      121 tcggacacgt gggccggtcg ctgggtgcgg gcaccgacca tcgccgagga tgccattctt
      181 ctggtagatc gatacagatc ggcgatcccc gccgcgagct cgcggacact ggtctgggtc
      241 atgatgagga cgcccatccc catcgccgag gcgaggacga cgaaggctcc ccacatgcca
      301 aggactcgcc gcatcgggaa ggcgatcagt gagccgacgg cgcctccccg ctcctccacc
      361 agctccaggc tcggcgccag ggcaacggcc ccggtcatga ggtggaacaa ggccagggag
      421 ccgaggaagg tgacgatgcc accggcgatc aggcgcttgg caccgtctcg gggcttgccg
      481 acgatcatgg cgactccgat gccggccagg gccacgggga gggcgaatct ccacaccccg
      541 aacaggaagc ggctgccgcc ggccattccc tcaccaaccg ggccggccag atcgacgaag
      601 gcgagcaaga ccaggatggc gagcaccacg aggatcatgc cccagatgtc gtcggtctga
      661 cgcccgaggg tgttgcgcac cgaaccgcgg accgccacca gcttttggcg gatcgcgctc
      721 aggggcgcag tcttcttttt cttcgctgtg gacacccgtc tccgcccgga ggaccccttc
      781 cgggtgggtg tcctggttcc catgtgctca gaaacaatac atgggagttg gatttggtcg
      841 tggaatggtc agccggcccc ggtctcggcc agaacctcgg cgaaatgacc ccgcctgccg
      901 gcgcgaggtg gtgtggtgtc tcttcgccta gagctccatc acaaccggca cgatcacggg
      961 ccggcgggcc gtctcggcgc ggatgacccg cccgacggcc tgacggatcg tcttctgcag
     1021 ctcgccgatg tcggatcgac cggaagcgtg ctcgccgatc gcctcggtga cggcatttgc
     1081 cgccttggcg agcacggtgc ccgggtcatc catgaggccg tgactgtcca gatccggccc
     1141 atacagcacc tcgccggtgt cgtggtcgat tccgaccgtg accacgacca cgccctcgtc
     1201 ggcgagatgg gagcggtcac gcagcaccgc cgactggacg tctccgacgg cagagccatc
     1261 gagatagaca taaccggccg ggacggccct cctctggacc tgggtcttct tgcccgcgag
     1321 gatcacccgg tccccgtctt cgaggacatc gacgtgtcgc actcccatct tgcgcgccag
     1381 ctcggcgtgg gcgtggagat gccggtattc cccgtggacc ggcacgaagg cctgggggcg
     1441 aaccagattg agaaaggtga gaagctcctc ccggttggcg tgcccggaga cgtggacatg
     1501 ggcattggtc ccatgaacga cgagcgcgcc cctgcgaatc aggttggaga tcactttcga
     1561 gactgcggtc tcgttacccg gtatcggctt ggccgagatg agaacggtgt ctccctcacc
     1621 gagctcgacg aacttgtgct ggccctgcga catcagcgac agcgctgcga acggctcccc
     1681 ctgactgccg gttgtgatga ggagctgttg attcgccgga agctcggcca aatccttgat
     1741 gtccatcacc gagttcgggg gcacgtccag ctctttcagc tccgcggcga gagcggtgtt
     1801 tcggtgcatg gagcggccga agaaggccac cttgcgaccg ttgcggatcc cggcgtcggc
     1861 tatctgctgg acacggtgga tgtgtgaggc gaagcaagcg gcgatgacgc gtccgccggc
     1921 atcccggatg acatcggtga tgggctgacc gaccgagctc tccgatggtt cgaaccccgg
     1981 tctctcggca ttggtggagt cggcgagcag gagacggacc ccgcgccggc ccagggtggc
     2041 gaaggctggc aggtcggtcg gcacgccatc gatcggtgtc gggtcgagct tgaagtctcc
     2101 cgagtggagg atgacgccct cgggtgtctc caccgccacg gcggtggcat cgggaaccga
     2161 gtgactgacc gggatcaact ggaaacggaa cggggtgtga tcgaaccact gcttgacctg
     2221 gacctgacgg aggtcggcct ccacgccgag ctcatcgatc cggccccgtg ccagctcgat
     2281 ggtgaatttg gtcccataga cggggacgtt cacctcccgc agaaagtagg cgagggcccc
     2341 ggtgtgatcc tcgtggccat gggtgaggac gacacattcc acgtcgcccg atctctctgt
     2401 gagccacgac cagtcgggga acaccagatc gacaccgagc atgtcctctt cggggaacat
     2461 caggccacag tcgatcaggg cgatccttcc ctcgacctct actgcagcgc agttgcggcc
     2521 gatttccccg agaccgccga gaaatgtgac cgagacgctc acaagccctg aagggcgccg
     2581 accgccttct cgacggcgac cagggtctcc tccgacgcct tcaccagggg aagccgcaca
     2641 tcgccaaccg gctcccagag ccgatccatg gcgcccttca ccggtgtggg gttggtctcg
     2701 aggaaacatg cctcgcagag aggcagaagc aaatggtgca ggcggcgtgc ctcgacgaca
     2761 tcacccgaca gggccgcccg gaccatggcg gagacggccc tcccggcgag atgggacacg
     2821 accgagacca cgcccaccgc accgaccgcc atcatcggcc atgtgaaaga gtcctggccg
     2881 gagtagacgg tcaggccggg gacccgattg atcgtgtgac cggtgaactc gatgtcgagg
     2941 acggcatcct tgacggcgac gatgctctcg tgctcggcga gtcgggccaa cgtgtcgatc
     3001 tcgatcctcg atccggtgcg ccccgggatg ttgtagagca tcaccggcag gccgacatcg
     3061 gctatcgcgc tgaagtgtga caccagtccg gcctggtcgg gcttgttgta gtagggcgca
     3121 acagccagga tcccggcgca cccgagatcg gacgccttcg ctgtgagctc gacagattcc
     3181 gcggtgttgt aggtgccggt cccggcgatc acgaatgtgt ccttgccggc gaccgcgtcg
     3241 acgactgtct tgtagagagc gactttctcg acactgctca gcgtcggcga ctcaccggtg
     3301 gtcccggtca cgacgagagc atcgcttccg ttgtcggaga ggaatcgggc gagacgccag
     3361 gccttctcgt gatccacctc ccccgaggca gcaaagggag tgatcatggc cgtggccaca
     3421 tggccgaacg gcgcagacgg gaatgagctc accggaccca ggttaccgat cgatccacta
     3481 gaacacccgc atggagttgc gactgcccgc tccgtgcctg gtcgttctgg tcggcgcgtc
     3541 cagttcaggc aagagcacct gggcgggccg ggccttcaaa gagacggagg tcgtgtcgtc
     3601 ggatcggctc cggggaatcg tgggctcggg cgaggatgac cagcaggcgg gcaccgctgc
     3661 tttctcgatc ctggagcaga tcgtcgagga gcgaatgcga cgccgcctga ccacggttat
     3721 cgacaccctt ggcttcgaca gggaaaagcg ccggcgatgg gtcgccctgg cccacgaggc
     3781 cgatctcccc gcctttgccg tcgtgttcga cacacccccc gagcaggctc gggtgcggaa
     3841 tgcagaacag gttcggccca tcccgaagac cgtcctcgac aagcagctcg ccaggtttcg
     3901 ggtggttcgg acggagatcg acgaggacgg attcgaccgc gtgctagtcg aacagccggt
     3961 ggctctggtc gctccctcga tg
//
LOCUS       DASTXO010000091         4663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_225937, whole genome shotgun sequence.
ACCESSION   DASTXO010000091 DASTXO010000000
VERSION     DASTXO010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225937"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VFU96_01800"
     CDS             <1..568
                     /locus_tag="VFU96_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEU5112018.1"
                     /translation="AWDELRTLSPMHGGMSYKRLDESGGLQWPCPTEDHPGTLFLHGR
                     LWEEETGPKAPFSVVEWVPPVDTLSDEFPVRLTTGRRLDSYNTGVQSGRYPSPRRIHG
                     VLDLSPEDALAFGVDEGETVRVVSRRGSIEAPVRVDRSLRPGLAFMAVHFPDDTDVNQ
                     LTIDAWDPKSGTAEFKATAVRIETLVST"
     gene            568..2391
                     /locus_tag="VFU96_01805"
     CDS             568..2391
                     /locus_tag="VFU96_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018332443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEU5112019.1"
                     /translation="MDLRNLLDQPTATERAAVDDLLGPPESGWDGGRFTGLDGHVAYG
                     GHAARARRNLLLPALHAVQDAAGSVSKGALGYISERLTVPPAEAYGVATFYSLINTRP
                     RSPTLIHVCDDIVCARYGADELKKSLEQELGPAGSAGSGIGWMPSPCLGQCDRAPAAL
                     AHTAGSGYRNVAPTDAAGLIGLAFDELPPGLIHQDRDDLRLLRRIGESDPERLDSYRD
                     NGGYAGLEKAIAMGRDGLIAALDESGLRGRGGAAFPIGIKWRGVADAGGDAKFIIANG
                     DESEPGTFKDRILMEGDPFAVIEAMTIAGMATGATKGFAYVRGEYPVAERRLANAIDQ
                     ARSAGLLGPDVAGAGFAFDVEVRRGAGAYICGEETALFASIEGQRGEPRQKPPFPTEK
                     GVFGKPTSVNNIETLVAVLAVLEIGAAEFAAIGTERSTGPKLFCISGDVTRPGLYEVP
                     FGTTLRQLIALAGGVRDDAAVGAVLIGGAAGSFVDPGTLDMELTFEGAAAAQTGLGSG
                     AVIVFDDTTDFGPVLRRIAQFFRDESCGQCVPCRIGTVRQEESLARLAAGNPLGSVTE
                     ERQRLDDLALVMTDASICGLGQTAASAVQSAFRLGLPGAAR"
     gene            2388..3314
                     /locus_tag="VFU96_01810"
     CDS             2388..3314
                     /locus_tag="VFU96_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017571176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU5112020.1"
                     /translation="MTAISDSVSTLTARVDGAEIEIAVGSTVLDACQEAGADIPTLCY
                     GPTMTAANACRVCVVEIEGSRTLVPSCSRQLEDGMEITTGSDRARHSRRLVMELLGSA
                     SELDRAGARLDHWMTDAGARPDRFGMPVKESPREPPAPGHHHPADPDYAATVAEPAKI
                     EDELYVRDYGRCILCYRCVDACGVEHQNTFAIAVAGRGFDARISTEFDVALPESACVY
                     CGNCIAVCPTGALAGRIEWDMREAGAWDESRQEVTRTVCSYCGVGCNLDLHTQDGQIV
                     SVTSPHDHDVTLGNLCIKGRFGWMYVSSGRGD"
     gene            3304..4155
                     /gene="fdhD"
                     /locus_tag="VFU96_01815"
     CDS             3304..4155
                     /gene="fdhD"
                     /locus_tag="VFU96_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEU5112021.1"
                     /translation="MATEAVSSRRVIRVRGEAARVEDELVVEEPVELRLDGTALAVVM
                     RTPGNDAELALGFAITEGIVTGPNEVASVVDLGEGRWDVRLAEGVRVDPSRFQRNFYA
                     TSSCGVCGKASIDAIRVAGSVPPHGPVVTRETLLGLPGRLIAEQPAFRSTGGIHAAAA
                     FDVDGSLVAVREDVGRHNAVDKLVGHLASIRWPIDQLGLLVSGRVSFEIVQKAAVAGL
                     SLVCGVSAASSLALDLAEEFHMTVIGFLRDNGFTIYTGEERVQLLPEDHSGGTLGDAD
                     DLGSGPT"
     gene            complement(4152..>4663)
                     /locus_tag="VFU96_01820"
     CDS             complement(4152..>4663)
                     /locus_tag="VFU96_01820"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEU5112022.1"
                     /translation="ELELVRQSAVQGGAFAAVTTEHHARGGEGARALAEAVVEASSQP
                     SEFDFLYPLDRSIKEKIETIATEIYKAGSVEYTQQAETQIADFERSGFGYLPICMAKT
                     HLSISDDPSLKGAPHGYELTVREVRASVGAGFIYPLLGEMSTMPGLGATPGFMKVDID
                     ETGEVVGLH"
BASE COUNT          769 a         1537 c         1560 g          797 t
ORIGIN      
        1 ggcttgggac gaattgcgca ctctctcgcc gatgcacggc gggatgagct acaagcggct
       61 cgacgagtcg ggcgggctgc aatggccctg ccccaccgag gaccatcccg ggacgctctt
      121 cctccatggc cggctctggg aggaggaaac cgggccaaag gcgccattct ccgtcgtcga
      181 atgggtgcca ccggtagaca cgctgagcga tgagtttccc gtgcgcctca ccactggccg
      241 tcgtctcgac tcgtacaaca ccggtgtgca gagcggacgc tatccatcac cccggcgcat
      301 ccatggagtt ctcgatctct ccccggagga cgcgctcgca ttcggagtcg acgagggcga
      361 gacggtgcgg gtggtgtcac gacgaggcag catcgaagct ccggtcagag tcgaccgctc
      421 gcttcggccc ggcctggcct tcatggcggt ccatttcccc gacgacaccg acgtgaacca
      481 gctcacgatc gacgcctggg accccaagtc gggaaccgcc gagttcaagg caaccgcggt
      541 gcggatcgag acgctggtct ccacataatg gacctccgca acctgctcga tcagccaacc
      601 gcgaccgaac gcgccgccgt cgacgatctg ctcggaccac ccgagtcggg gtgggatggc
      661 gggcgcttca ccgggctcga cgggcatgtc gcctacgggg gccatgccgc ccgggcacga
      721 cgcaacctcc tgctgcccgc gctgcacgcg gtgcaggacg ccgcaggctc ggtgagcaag
      781 ggagcgctgg gctacatctc ggagcgactc accgttcccc ctgccgaggc gtacggcgtt
      841 gccaccttct actccctgat caacacccgg ccacgctcgc caaccctgat ccatgtctgc
      901 gacgacatcg tctgtgctcg gtacggggcg gatgagctga agaagtccct ggagcaagag
      961 ctggggccgg ccggctccgc aggctcggga atcggctgga tgccgagccc gtgtctcggc
     1021 cagtgtgacc gtgctcccgc ggcactggcc cacaccgcag gctccggcta tcgcaacgtt
     1081 gcccctaccg acgccgccgg tctcatcggc ctcgctttcg atgagctgcc tccggggttg
     1141 atccatcagg atcgggacga cctgcgtctt cttcgccgga taggtgagag cgaccccgag
     1201 aggctcgatt cctaccgaga caacggaggc tatgccgggt tggagaaagc catcgcgatg
     1261 ggtcgggacg gtctcatcgc cgcgctcgac gaatcaggtt tgagaggtcg ggggggagcg
     1321 gctttcccca tcgggatcaa atggcgcggg gtggccgacg ccggcgggga tgccaaattc
     1381 atcatcgcca acggcgacga atcggaaccg ggcaccttca aggaccggat cctgatggag
     1441 ggcgacccat tcgcagtcat cgaggcgatg acgatcgccg ggatggccac cggggccacg
     1501 aagggtttcg cctatgttcg gggtgaatat cccgtagccg agcgtcgtct ggccaacgca
     1561 atcgaccagg caaggagcgc cggtctcctc ggccccgatg tcgccggggc cggtttcgcc
     1621 ttcgacgtcg aagtcagacg gggggcgggg gcctacatct gcggcgaaga aacggcactg
     1681 ttcgcctcta tcgagggtca gcgtggcgag ccaaggcaga agccaccttt ccccacggaa
     1741 aagggggtat tcggcaagcc cacctcggtc aacaacatcg agacccttgt ggcggtgttg
     1801 gcggtgctcg agatcggagc ggcggagttc gcggccattg gcaccgagcg ctccaccggc
     1861 cccaagctct tctgcatctc gggcgacgtg acccggcccg ggctctatga ggtcccgttc
     1921 ggtaccaccc tgcgccaact gatcgcgctg gccggcggtg tgcgtgatga cgccgcggtg
     1981 ggagcggtcc tgataggggg tgccgcaggg agcttcgtcg accccgggac cctcgacatg
     2041 gagctgacct tcgagggggc agccgcagct caaaccgggc tcggctccgg ggcagtgatc
     2101 gtcttcgacg atacgaccga cttcggcccg gttctgcgcc gcatcgccca attcttcaga
     2161 gacgagtcgt gcgggcagtg cgtaccctgc cggatcggca cggttcgcca ggaggaatcc
     2221 ctcgcccggc tcgccgcggg caatcccttg gggagcgtca ctgaggagcg gcagcggctc
     2281 gacgacctgg ctttggtgat gaccgacgcg tcgatctgcg gcctgggcca gaccgccgca
     2341 tccgccgtgc agtcggcttt ccgtctcggt cttccgggag cggcccgatg accgcaatca
     2401 gcgactcggt ctcaacgctc acggcccgcg tcgatggggc ggagatcgaa atcgccgtcg
     2461 gctccaccgt tctcgacgcg tgtcaggagg cgggggcgga catcccgacg ctctgttacg
     2521 ggccgaccat gaccgcggcc aacgcctgtc gcgtatgtgt tgtggagatc gaaggctccc
     2581 gcactctcgt gccctcctgc tcccgccagc tcgaagacgg gatggaaatc acaactggct
     2641 ccgatcgcgc ccgacacagc cgtcgattgg tgatggagct gctgggctcc gcctcggagc
     2701 tggaccgggc cggtgcccgc ctcgatcact ggatgaccga tgccggggca cggccggacc
     2761 gcttcggcat gcccgtgaag gagtcgccac gggagccacc ggcgccgggg catcatcatc
     2821 cggccgatcc cgactacgcc gccacggtgg ccgagccggc caagatcgag gacgagctct
     2881 acgttcgcga ctatggccgc tgcatcctct gctaccgctg tgtcgacgca tgcggggtcg
     2941 agcaccagaa cacattcgcc atcgcggtgg caggacgcgg cttcgacgca cggatctcca
     3001 ccgagttcga cgtagccctg cccgagtcgg cgtgtgtcta ttgcggcaac tgcatcgctg
     3061 tttgtccgac gggggccctc gccgggagga tcgagtggga catgagagag gcaggggcct
     3121 gggacgagtc gcgccaggaa gtcacgcgga cggtctgctc ctactgcggc gtgggctgca
     3181 atctcgatct ccacacccag gacggccaga tcgtcagcgt cacctcgccg cacgaccacg
     3241 acgtcacact gggcaacctt tgcatcaagg gacggttcgg ttggatgtac gtcagcagcg
     3301 gccgtggcga ctgaggcggt cagcagccgg cgggtgatcc gggtgcgggg ggaggcggca
     3361 cgggtcgaag acgaactggt cgtcgaagag ccggtcgagc tccgactcga tgggaccgcg
     3421 ctggccgtgg tcatgcgaac gcccggcaac gatgccgagc tggcgttggg cttcgccatc
     3481 accgagggaa tcgtcaccgg ccccaacgaa gtggcgtcgg tggtcgacct cggcgagggg
     3541 cgttgggatg tccggctcgc cgagggcgtc agggtcgatc cgtctcgatt ccagcgcaac
     3601 ttctatgcca cctcctcgtg cggggtctgt ggcaaggcat cgatcgacgc catccgggtg
     3661 gcgggctcgg tgccgccgca cggaccggtg gtcacacggg agaccttgct cggcttgcca
     3721 gggcgtctca tcgcggagca accggcgttt cgctccaccg ggggtatcca tgccgccgcc
     3781 gcgttcgatg tggacgggag tctcgtggcg gtgcgagagg atgtgggccg acacaatgcg
     3841 gtggacaagc tggtcggtca tctcgcgtcg atccgctggc cgatcgatca gctcggcttg
     3901 ctcgtctcgg gcagggtctc gttcgagatc gtccagaagg cagcagtggc cggcctctcg
     3961 ctggtatgtg gcgtctccgc tgcatccagc ctcgccctcg atctcgccga ggaattccac
     4021 atgacggtga tcgggttcct ccgcgacaac gggttcacca tctataccgg ggaggagagg
     4081 gttcagctac tccccgagga ccacagcggc ggaacactag gtgacgccga cgatttgggc
     4141 tcgggcccta cctagtggag acccaccacc tcaccggtct catcgatgtc gaccttcatg
     4201 aagccgggcg ttgcccccag accgggcatg gtgctcatct ccccgagtag ggggtagatg
     4261 aacccggccc cgaccgatgc cctgacctcc cgcaccgtca gctcataacc gtgtggagcg
     4321 cccttcagtg aagggtcgtc gctgatcgaa agatgggtct tggccatgca gatcggcaga
     4381 tagccaaacc ccgaccgctc gaaatcggcg atctgggtct cggcttgctg tgtgtactcc
     4441 accgatcctg ccttgtagat ctccgtggcg atggtctcga tcttttcttt gatcgagcgg
     4501 tcgaggggat agagaaagtc gaactcgctc ggctggctac tcgcctcgac cactgcttcg
     4561 gccaacgcgc gcgcgccttc cccacctcgg gcatgatgct cggtggtgac cgcggcgaac
     4621 gcaccccctt gcaccgccga ctgcctgacc agttccagct cgt
//
LOCUS       DASTXO010000092         4193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1257943, whole genome shotgun sequence.
ACCESSION   DASTXO010000092 DASTXO010000000
VERSION     DASTXO010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257943"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="VFU96_01825"
     CDS             <1..1086
                     /locus_tag="VFU96_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEU5112023.1"
                     /translation="EVPAASLADDAPIYDRPMRRPERLDRLWANPPRNLEDDPAKILM
                     KLLDDPALGSTAWVHEQYDHMLFLNTVVGPGSDGSLIRVRGTRKGLAVSTDGDGRLCH
                     LDPRGGSSRLVYEAALNVAVMGARPLAVVDNLNFGNPEKPEVMWQFKESVEGISVACE
                     ALGIPVVGGNVSFYNETDGVDIHPTPVVGLLGLADPMPDIPPRLSRAEEGMEIWEIGP
                     VPTGDLSGSAAQRVMNRELTGKPTSPDPGAAQRVVALAADLAHRAPVLHDISDGGLAA
                     AVAEVCIASQIGATITEASNGRLFSEDPHRFIAVLEPGAVELPEDFSRKVGTVGGDAL
                     TLGGSDPVEIRALTETSQDAIPRRMTG"
     gene            complement(1087..1752)
                     /locus_tag="VFU96_01830"
     CDS             complement(1087..1752)
                     /locus_tag="VFU96_01830"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU5112024.1"
                     /translation="MNQDINLSRDQRVAAVGRLVESLRALQLAGDLMDQAFADFVGIN
                     RNDLRCIDIVDQRGRMTAGELAREAGLTTGAVTALVDRMEAAGLLRRSYDSKDRRKVW
                     VELTTEAQKLGDEVYGPLNQTGQMHLDSLSDEQLFTIIGFLDLSRRITMESVEAIRGR
                     TATKAVPLRYRLEQAKMLKNDAKTLLKTIKNEVKGLTAVVIDIHGSKWEQDERGRWVE
                     RSD"
     gene            1804..2787
                     /locus_tag="VFU96_01835"
     CDS             1804..2787
                     /locus_tag="VFU96_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5112025.1"
                     /translation="MIESRGLRRTYKTKKQEIEAVRGVDFTVDAGEIVGFLGPNGAGK
                     TTTLRMLTTLLEPTGGEATVAGCDLRADPVGVRRRIGYVAQGGSTDPEARAGEEIVDH
                     ARMYGIDAAVANSNGQALFDQLDLEGLWARQCKTLSGGQRRRLDIAMGLVHQPGLVFL
                     DEPSTGLDPQSRANLWEHVARLRSEMDTTVFITTHYMDEADVLCDRILVIDDGAIVAQ
                     GTPDELKRKVAGDSVILSFEQQRSTVVVAELVSRLDGATDPSIEGNEVRFQVPDGASA
                     LPVLLRAMVEHDLDATGVEVRRPTLDDVFLTMTGRSLREDAIAGTTDQAAA"
     gene            2802..3566
                     /locus_tag="VFU96_01840"
     CDS             2802..3566
                     /locus_tag="VFU96_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5112026.1"
                     /translation="MRFLTDTWLVFSRAMRLSLRNPVWVILGLMQPILYLILFGPLLE
                     PVASAPGFPPGSAWQVFVPGLLVQLGIFGAAFAGFGLIAEWRAGVVERMRVTPASRGS
                     LLLGRVLRDVAVILVQGALLSVVAVSFFGLEAPIEGLAIGVAIVGLLGGAFSALSYGA
                     ALKLKSEDSFAPVVNGLILPILLLSGILLPMTLAPGWLQTMADVNPLTHIVDGVRALY
                     RGDVGSTDAWLGAALTLGLLVVGVFYGARVFRKESA"
     gene            3613..>4193
                     /locus_tag="VFU96_01845"
     CDS             3613..>4193
                     /locus_tag="VFU96_01845"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004012685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEU5112027.1"
                     /translation="MARSIGILTAGGDSPGLNAAIRAVGKAAQRAYGMHVIGFYDGFL
                     GLMQNRFVRFDSDSLSGILTRGGTVLGTSRAKVDKMEMGDGRRMDMRSLMRDVVDQHD
                     LEDLVVLGGDGTMRNALKLEDVGINVLALPKTIDNDVTGTDVTFGFDTAMGIATEAID
                     RLHSTAHSHHRIILAEVMGHEVGWLAMGAGLAGG"
BASE COUNT          716 a         1377 c         1340 g          760 t
ORIGIN      
        1 gaggtccccg ccgcctcatt ggccgacgat gcaccgatct acgaccgtcc gatgcgacga
       61 ccggaacgcc tcgacaggct gtgggccaac ccgccccgga atctcgagga cgacccggcc
      121 aagatcctca tgaagctcct cgacgatccg gcgctcggct cgacggcctg ggtccacgag
      181 cagtacgacc acatgctctt cctcaacacg gtggtggggc ccggatcgga cggttccttg
      241 atccgggtca gaggcacacg gaagggcctg gccgtatcca ccgacggcga cggccggctc
      301 tgtcatctgg atccaagagg tgggagctcg agacttgttt atgaggccgc gctcaacgtg
      361 gccgtcatgg gtgcgcgtcc gctggccgtg gtcgacaacc tgaatttcgg gaatccggag
      421 aagcccgagg tcatgtggca gttcaaggag agcgtcgagg ggatctcggt ggcctgtgag
      481 gcactcggga tccccgtggt gggaggcaac gtctccttct acaacgagac ggatggcgtc
      541 gacatccacc ccactccggt ggtcggcctc ctcgggctcg ccgatccgat gccggatatc
      601 ccaccccggc tctcccgcgc agaggaaggg atggagatct gggagatcgg ccccgtaccg
      661 acaggcgacc tgtccggctc ggccgcgcag agggtcatga acagggaatt gaccgggaag
      721 ccgacctcac ctgaccccgg agccgcccaa cgggtggtgg cgctcgccgc cgacctggct
      781 catcgagctc cagtcctgca cgacatctcc gatggagggc tggcagcggc ggtggccgag
      841 gtatgcatcg cctcgcagat cggagccacg atcaccgagg cctcgaacgg gcggttgttc
      901 tccgaggatc cgcaccggtt catcgcggtg ctcgagccgg gcgcggtcga attgcccgaa
      961 gacttcagcc ggaaggtggg aaccgtcggc ggcgacgcgc tcacactcgg aggctctgat
     1021 ccagtcgaga ttcgggcgct caccgaaaca tcccaagacg ccattccccg tcgcatgacg
     1081 gggtgatcag tccgatcgct ccacccagcg acctcgctcg tcctgctccc atttcgatcc
     1141 gtggatgtct atgaccacgg cagtcagacc cttgacttcg ttcttgatgg tcttgagcaa
     1201 agtcttggcg tcgttcttca gcatcttcgc ctgctccagg cggtagcgca gcggaaccgc
     1261 cttggtcgcg gtgcgcccgc ggatcgcctc cacactctcc atcgtgatgc ggcggctcaa
     1321 atcgaggaag ccgatgatcg tgaacagctg ctcatcgctc agcgaatcga ggtgcatctg
     1381 cccggtttgg ttcaggggtc catacacctc atcacccaac ttttgcgcct cggtggtgag
     1441 ctcgacccat accttgcgcc ggtctttgga gtcataggac ctgcgcagga gacccgccgc
     1501 ctccatcctg tcgacgaggg cggtcaccgc accagtggtc agccccgcct cgcgcgccag
     1561 ctcgcctgcg gtcattcggc cacgctggtc gacgatgtcg atgcagcgaa gatcgtttcg
     1621 gttgatgccg acgaaatcgg cgaaagcctg gtccatgagg tcgccggcca actgcagggc
     1681 ccggagtgac tcgaccagcc ggcccaccgc cgccacccgt tgatctcttg acaagttgat
     1741 atcttgattc ataagatact caccacttga agtatagtgc aaggagtggg tgccatgcca
     1801 gagatgatcg aatcccgtgg gttgcgacgg acctacaaga ccaagaagca ggagatcgag
     1861 gcggtgcgcg gggtcgactt cacagtcgat gccggtgaga tcgtcggctt tctcgggccc
     1921 aatggcgccg ggaagaccac cactctgcgg atgctcacca cgcttctcga gccgaccggt
     1981 ggcgaggcca cggttgccgg gtgcgaccta agggccgatc ccgtcggggt ccgccgtcgc
     2041 atcgggtacg tggcgcaagg tggatcgacc gaccctgagg ccagagccgg tgaggagatc
     2101 gtcgatcacg cccgcatgta cggaatcgac gccgcggtcg ccaatagcaa cggacaggcg
     2161 ctcttcgacc agctcgacct cgagggtcta tgggcgcggc agtgcaagac actctccggc
     2221 ggacaacggc gccgactcga catcgcaatg ggcttggttc accaacccgg tctggtgttc
     2281 ctggatgagc cctccacggg gctcgacccg caatcgcggg caaacctgtg ggaacacgtc
     2341 gcgcgactgc ggtccgagat ggacaccact gtgttcatca cgacccatta catggacgag
     2401 gccgacgttc tctgtgaccg catcctcgtc atcgacgatg gcgccatcgt ggcccaaggc
     2461 actcccgacg agctcaagcg aaaggtggcc ggcgactccg tgatcctgag cttcgagcaa
     2521 cagagaagca ctgtcgtggt tgccgagctc gtctcacgtc tcgatggcgc caccgacccg
     2581 tcgatagaag gcaacgaggt gcgattccag gtgcccgacg gggccagcgc gctcccagtg
     2641 ctcctccggg cgatggtcga acacgacctc gacgccaccg gtgtcgaggt gcgtcggccc
     2701 accctcgatg acgtgttcct caccatgacc ggtcgctcac tccgcgaaga cgcgatcgcg
     2761 ggcaccaccg accaggccgc ggcctgaaag gaagaatgat catgcgattc ctcaccgata
     2821 cgtggctcgt gttcagccgc gccatgcgtc tctcgctgcg aaacccggtt tgggtgatcc
     2881 tcgggttgat gcagcccatc ctctatctga tcctgttcgg gccgctgctg gagcccgttg
     2941 cgtccgctcc cggtttcccg cccggctcgg cctggcaggt gttcgtgccc gggctgctcg
     3001 tccaactcgg cattttcgga gctgctttcg ccggcttcgg gttgatcgcc gaatggcggg
     3061 ccggggtcgt cgagcgaatg cgagtcaccc cggcgagccg agggtccctc ctcctcgggc
     3121 gggtgctgcg agatgtcgcc gtcatcctcg tccaaggggc tctcttgtcg gtcgtggcag
     3181 tctcgttctt cgggctcgag gcgccgatcg agggtctcgc catcggcgtc gccatcgtcg
     3241 ggcttctcgg cggtgccttc agcgccctgt cctacggggc ggcactgaag ctgaagagcg
     3301 aggattcctt cgccccggtg gtcaacggcc tgatcctccc catcctgctc ctgtcgggga
     3361 tcctcctccc catgaccctg gccccgggct ggctgcagac catggcggac gtgaatccat
     3421 tgacgcacat cgtcgacggg gtccgagcgt tgtatcgcgg cgacgtgggc tcgacggacg
     3481 cctggctcgg ggctgcactc acccttggcc tgctcgtcgt cggcgtgttc tacggagcgc
     3541 gggtcttccg caaggaatct gcctgaggat tccggtatcg gccgcgccct gctcggccga
     3601 taccatccca tcgtggcgag aagcatcggg atattgaccg ccggcggcga cagccccggt
     3661 ctgaacgccg ctatcagggc ggtaggtaaa gccgcccagc gcgcctatgg gatgcatgtc
     3721 atcggcttct acgacggctt cctggggctg atgcagaatc gtttcgtccg attcgactcc
     3781 gattcgctct ccggcatcct gaccagggga gggacagtcc tcgggaccag ccgagccaag
     3841 gtcgacaaga tggagatggg cgacggcagg cgcatggaca tgcggtcgct gatgcgtgac
     3901 gtcgtcgatc agcacgatct cgaagatctc gtggtcctcg gcggcgacgg cacgatgcgc
     3961 aatgccctca agctggagga cgtcggcatc aacgtgctcg cgcttcccaa gacgatcgac
     4021 aacgacgtca ccggcaccga cgtcacgttt ggattcgaca ccgcgatggg catcgccacc
     4081 gaggcgatag accggctgca ctccaccgca cactcccatc atcggatcat cctcgccgag
     4141 gtgatgggac atgaggtcgg atggctggcc atgggggccg ggctcgccgg cgg
//
LOCUS       DASTXO010000093         2946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1294880, whole genome shotgun sequence.
ACCESSION   DASTXO010000093 DASTXO010000000
VERSION     DASTXO010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294880"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..979)
                     /locus_tag="VFU96_01850"
     CDS             complement(194..979)
                     /locus_tag="VFU96_01850"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEU5112028.1"
                     /translation="MSLDLWEPPVAIAHRGSRLLWPENTMEAFSGAVELGFRHLETDL
                     RVTGDGVLVCLHDPTVDRTTNGMGPVSELTFDQLANLDAGYRHMGVDGRVFRGRGIRV
                     PTLEELVLTYPEVSFVVDLKTDNLVGPLIELIERVELYERLIVGSFSDRRLEEFREAT
                     AGRVATSTGSTAARSWLMASRIGRGVIGEAAALQLPKHSRGLRVVDRRLIESAHAQGL
                     QVHVWTVNNRSEMEELLDLGVDGIITDRPDVLKGVLEERGQWG"
     gene            complement(1043..1336)
                     /locus_tag="VFU96_01855"
     CDS             complement(1043..1336)
                     /locus_tag="VFU96_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU5112029.1"
                     /translation="MNKSEMADKLADRADISKGKAAEIIDHIFSTKSGEGIIAAELDA
                     GRKVNITGFGNFATKHRSARQGRNPATGETITIAAKRYAHFSASKGLKDRVAE"
     gene            1457..2233
                     /locus_tag="VFU96_01860"
     CDS             1457..2233
                     /locus_tag="VFU96_01860"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU5112030.1"
                     /translation="MSVDYVLEDDLAIVTLNRPDRYNAIDASLGNGLIQAARRAGEEA
                     RALVLTGSGKAFCSGADLNDLMADYESDDGPDLSGLLEDVFHPALYSLLECQVPVIGA
                     INGVAAGAGLGLALACDLRVMAEEAFLTSAFTAIGLVPDSGTTWWLSHHLGVSRALDV
                     ALTNRRIGAEEARDLGLCVDVVPADQLLDRARSLADQMADLVPDSVVTTRRLIREAAG
                     VSLDEALRAEQAEQGRLGKTSEHREGVMAFIEKRKPDFRA"
     gene            complement(2270..2680)
                     /locus_tag="VFU96_01865"
     CDS             complement(2270..2680)
                     /locus_tag="VFU96_01865"
                     /inference="COORDINATES: protein motif:HMM:NF023010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 family protein"
                     /protein_id="HEU5112031.1"
                     /translation="MPSLELFCTIKAEMGRKIIGNAPAGMRIDFPFEGTATGPHWDGE
                     RPVSGIDYVTIRSDGNMDLDIHATIGEKRETVAYSGSGVSIVNPDQSAEPRELLTFQT
                     GNEDLAWLNNEVGLAFGKGEAGSLMVEVYLARPE"
     gene            complement(2712..>2946)
                     /locus_tag="VFU96_01870"
     CDS             complement(2712..>2946)
                     /locus_tag="VFU96_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEU5112032.1"
                     /translation="GSTAIAAIRVLRARDAHAVWLAVPVAPADAIPVMEMVADRMIVV
                     EHPRRFMAVGAWYRDFTPTPDADVIELLRRSRN"
BASE COUNT          545 a          938 c          904 g          559 t
ORIGIN      
        1 gagcgaccac cgagtcggcg gtggtctcca cctccacgcg aaggtcgacg atcatggatt
       61 gatcggtcat cgagacgacg gatgccgaag ccgagccaaa gccggcccct acctcaaccg
      121 cgaaaaggac aacggcggcc agtctgatca tcggtgcgat ggtacctgtc acctcgggga
      181 ggcactcctc gcctcaaccc cactgccctc gctcctccag cacccctttg agcacatcgg
      241 gacggtcggt gatgatcccg tcgactccga gatcgagcag ttcttccatc tcagatcggt
      301 tgttgacggt ccagacgtgg acctggagtc cctgggcatg ggccgattcg atcaaacgcc
      361 ggtcgaccac tcggagcccc ctcgaatgct tggggagctg aagggccgct gcctctccaa
      421 tcactccccg cccgattctc gatgccatga gccatgacct ggctgcggtc gacccggttg
      481 aagttgcgac acgtccggcc gtcgcctccc ggaactcctc gagccgccgg tcggagaaag
      541 agcccacgat caggcgctca tagagctcga cacgttcgat caactcgatc agaggaccca
      601 ccaggttgtc ggtcttcaga tcgacgacga aggagacctc cggataggtg agcaccagct
      661 cctcgagggt gggaacccgg atcccccgtc cccggaagac gcgtccatcg acccccatgt
      721 gtcggtaacc ggcgtcgaga ttggccagct ggtcgaaggt gagttcggag acgggtccca
      781 ttccgttggt cgtgcggtcc accgtcggat cgtgaagaca gacgaggacg ccgtcaccgg
      841 tgaccctcag gtccgtttcc aggtggcgaa agccaagctc gacagcccct gagaaggcct
      901 ccatggtgtt ctccggccag aggaggcggc tgccccgatg ggcgatggcg accggtggct
      961 cccagaggtc gaggctcatg cccgggcaaa agtaatgggc cctgacagat gacagggccc
     1021 atcaaatccc ggattagggc gcttactcgg cgactcggtc cttcaggccc ttcgaggcgg
     1081 agaagtgggc atacctcttc gctgcgatcg tgatggtctc cccggtggcc gggttgcggc
     1141 cctggcgggc agagcgatgc ttggtggcga aattgccaaa cccggtgatg ttcactttgc
     1201 ggcccgcatc gagctccgcg gcgatgattc cctcgcctga cttcgtggag aagatgtgat
     1261 cgatgatctc cgcggccttg cctttggaga tgtccgccct gtcggcgagt ttgtccgcca
     1321 tctcggattt gttcacggcc cctccttgtg ggtcgacgtt cggcaccgtg tttctggtga
     1381 cgttcgagta accataggcg aaaaacccag cattgcttgg ttttccggcc cattagcctc
     1441 ttagccgagg aggaaaatga gcgtcgacta cgtcctcgaa gacgatctgg ccatcgtcac
     1501 tctcaatcgg ccggaccgat acaacgccat agatgcgtcc ctcggcaacg ggctgatcca
     1561 agcagcacgc cgagccggag aagaggcaag ggcccttgtt ctcaccgggt cgggcaaggc
     1621 gttttgctcc ggtgccgacc tcaacgatct gatggcggac tatgagagtg acgacgggcc
     1681 cgacctgtcg ggtttgctcg aggatgtgtt ccatcccgcc ctgtattcct tactcgagtg
     1741 ccaggtcccg gtcatcgggg cgatcaacgg cgtagcggcc ggggcgggcc ttggcctcgc
     1801 cctagcctgc gaccttcggg tgatggcgga ggaggccttt ctcacctcgg cattcacggc
     1861 catcgggctg gtgccagact cgggcacgac gtggtggctg tcacaccatc tcggagtatc
     1921 ccgcgccctt gacgtcgctc tgaccaatcg acggatcggc gcagaagaag cccgtgacct
     1981 ggggctatgc gtcgatgtgg tgcccgctga ccagctgctc gatcgggccc gatcattggc
     2041 agatcagatg gcggacctgg tccccgattc cgtggtcacc accaggcgtc tcatcagaga
     2101 ggctgcagga gtctccctcg acgaggcatt gcgagccgag caggccgagc agggtcgtct
     2161 cggaaagaca tcagagcatc gagaaggcgt gatggccttc atcgagaaga gaaagccaga
     2221 tttcagggct tgatttgctc catcatcgtc cgcgacgcgg cgagctctct cattcagggc
     2281 gggccaggta gacctcgacc atcagactcc cggcctcacc cttgccaaac gccagcccca
     2341 cctcgttgtt cagccaggca aggtcctcgt tgccggtttg gaaggtcaac agctcacggg
     2401 gctctgccga ttggtccggg ttgacgatgg agacgccgct gccggaatac gccacggtct
     2461 ctcgcttctc gccaatcgtg gcgtgaatgt cgagatccat gttcccgtcc gaccgaatcg
     2521 tcacgtaatc gatgccgctc accggtctct ctccgtccca atgcggacca gtggctgtgc
     2581 cctcgaatgg gaagtcgatc cgcatccccg ccggcgcgtt gccgatgatc ttcctaccca
     2641 tctcggcctt gatcgtgcag aacaactcca atgaaggcat gggctcgtgc tcctgggtcg
     2701 ggtagagaac gctagttccg ggagcgccgc agcagctcga tgacatcggc atccggagtc
     2761 ggggtgaagt cgcgatacca ggcgcccacc gccatgaatc ttctcggatg ctcgacaaca
     2821 atcatgcgat cggcgaccat ctccatgacg gggatcgcgt cagccggtgc cacgggcacc
     2881 gccaaccaaa ctgcgtgcgc gtcacgggca cgcaaaacgc gaattgcagc gattgcagtc
     2941 gagccg
//
LOCUS       DASTXO010000094         2869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1773850, whole genome shotgun sequence.
ACCESSION   DASTXO010000094 DASTXO010000000
VERSION     DASTXO010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1773850"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VFU96_01875"
     CDS             complement(<1..330)
                     /locus_tag="VFU96_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112033.1"
                     /translation="MALLLISAQSNAGPGEREQMLDGAAQQFARAGLERGQVLRIDVP
                     GRGAGEEGDGALRTELEPVIPLLQSGSLFGEPQGLQLVDAQNLQASESAVLEELIKTA
                     DLTTIEVV"
     gene            complement(379..1173)
                     /locus_tag="VFU96_01880"
     CDS             complement(379..1173)
                     /locus_tag="VFU96_01880"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU5112034.1"
                     /translation="MLDLGVTQLPLGEITYVMGVINVSPDSKNVHTVARSVDEALLMA
                     DRYRDWGATMLDIGGQSSHLDNPTIEVSDEIARVVPVIEALVAEGHLVAIDTWKPDVA
                     GAAVGAGAVIVNDTGGLTDPEMRQIVAQTGVATVAVYVEGDNPHDVGEIEIRDDKADR
                     TADVFRALLADLAGTGIVNVILDPGIALNYRGDYEAYTRMQLEVIADSEQLRALGKPV
                     LIPIPRKRDFHRVVAYISLALEHEADIIRVHDVAAACDLARLFGRA"
     gene            complement(1192..>2869)
                     /locus_tag="VFU96_01885"
     CDS             complement(1192..>2869)
                     /locus_tag="VFU96_01885"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEU5112035.1"
                     /translation="GAGEMAGNPHRGTVDVTTFRVVSGPGSPLLALGNAMRDRVIDRL
                     SPLEGGRALLAGFMVGDTSGVDEVDQSAMRRAGLSHFTAVSGSNVAIFLGLLYVVAGP
                     LGIGPKRRAVLGLFGLPVFAAATRFEPSVLRASAMAGLVLAGRLVGVVMETWQVISAA
                     VVALLLIDPGLVQSAGFQLSLAATTGVIVGTRWPTSGGRVARAVAVSVGAQVAVAPLL
                     MIHFGLIPLLSPLANLVAAPLVAASTVLGVLGVIGPAPLLDLGGALAQVVLGIARSAS
                     GLPQVGWLGLGIVLLAGLIHLLQPRLKGVLALLGAAAVTVLLLAPGSSLPDVGAVVLD
                     VGQGDAILLSGGPGQLALVDGGPDPVVLFENLTQYGANGLKLVVLTHAHADHALGLMA
                     LVGRIPIGEVWARIHPHETAVSTELLGALSTLGVQVVEPAPGDVYRLGALSLVVEGPI
                     REYASSNDQSIVLTVSGRDRSMLLAGDIETYAQADLGHLRADVLKVPHHGAGTSDPDW
                     FEGVGAEMAVISVGSNDFGHPVPWVINELEESGATVLRTDLEGDVIVPLG"
BASE COUNT          566 a          994 c          883 g          426 t
ORIGIN      
        1 cacgacctcg atcgtggtca ggtcagcagt cttgatcagc tcctcgagga ccgcactctc
       61 cgacgcctgc agattttgcg catcaaccag ctgcaaaccc tgtggctcgc cgaacagcga
      121 ccctgattgc agcagtggga tgacgggctc caactcggtg cgcaaggcgc cgtcaccctc
      181 ctctcccgca ccacggcccg ggacgtcgat cctgagcacc tgaccccgct ccaatccggc
      241 ccgcgcgaac tgctgcgccg ctccgtcgag catctgctca cgctcgccgg gccctgcatt
      301 cgattgggcc gagatgagaa gcaacgccat gacctgacgg taatcagacc tgccgacacg
      361 tttgggtctc gagatctatc aggcgcgacc aaacaggcgg gccagatcgc aggctgcggc
      421 gacatcgtga acgcggatga tgtcggcttc gtgctccaag gcgagcgaga tgtaggccac
      481 gacgcgatgg aaatcacgct tccgtgggat cggtatcaag accggcttcc ccagggcccg
      541 aagctgctcg gagtcggcga tgacctcgag ctgcattcgg gtatatgcct cgtagtcgcc
      601 gcgatagttg agggcgatcc ccgggtcgag gatgacgttg acgatgcccg tcccggccag
      661 atcagctagg agggctcgga agacgtcggc agtccgatcg gctttgtcgt ctctgatctc
      721 gatctcaccg acatcgtggg ggttgtcccc ctccacgtag accgccaccg tggccacgcc
      781 ggtctgggcg acgatctggc gcatctcagg gtcggtgaga ccgcccgtgt cgttgacgat
      841 caccgccccc gccccgacgg ctgcgccggc tacgtctggc ttccatgtat cgatggccac
      901 caggtgccct tccgcgacga gggcttcgat cacagggacc accctcgcga tctcgtctga
      961 cacctcgatg gtcggattgt cgagatgcga cgactgcccg ccgatgtcga gcatcgtggc
     1021 gccccaatcc cggtaacggt cggccatcag gagggcttca tcgaccgagc gcgcgacggt
     1081 gtgcacgttc ttcgagtcag gcgagacgtt gatgactccc atcacatacg tgatctctcc
     1141 caaggggagc tgagtgactc cgagatccaa cacgggccca gtctcccatc ctcacccaag
     1201 aggcacgatc acatccccct ccagatccgt gcgcaagaca gtcgcgccgc tctcttcgag
     1261 ctcgttgatc acccagggga cgggatggcc gaagtcgttg gatccgaccg agatcaccgc
     1321 catctcggca cccacccctt cgaaccagtc cgggtccgac gtgccggcgc cgtggtgcgg
     1381 caccttcaag acatcagccc gcaggtgtcc cagatcggct tgggcgtagg tctcgatatc
     1441 ccccgccagc agcatggacc gatcccggcc cgacaccgtc aacacgatcg actgatcgtt
     1501 ggatgatgca tactcacgga tcggtccctc cacgacgaga ctcaatgccc cgaggcgata
     1561 cacgtctccc ggcgctggct ccacgacctg tactcccagc gtcgagagtg ctccgaggag
     1621 ctcagtcgac acggccgtct cgtgggggtg gatgcgcgcc caaacctcgc cgatcgggat
     1681 tctcccaacc agggccatga gcccgagtgc gtgatcggcg tgggcatggg tcagcaccac
     1741 cagcttcaac ccgttcgccc cgtactgggt gagattctcg aaaaggacga ccggatccgg
     1801 gccgccgtcg acgagagcca gttgcccggg accacccgag agcagaatcg catcaccctg
     1861 accgacgtcg aggaccaccg ccccaacatc cggcagggac gatccagggg ccaaaaggag
     1921 aacggtgacg gcggcagcac cgaggagggc cagaaccccc ttgagccttg gctgcaacag
     1981 gtggatcaag cctgcaagga gcacgatccc gagccccaac cacccgacct ggggcagacc
     2041 cgaggcggat cgagcgatcc cgaggacgac ctgtgccaac gcccccccga ggtcgaggag
     2101 cggcgccggg ccaatgacgc ccaacacacc caggacggtc gaggcagcga ccaacggagc
     2161 ggccacgagg ttggccagcg gggagagaag ggggatcaac ccgaaatgga tcatgagcag
     2221 cggagcgact gcgacctgcg cgccgacact cacggccaca gcgcgagcca ccctcccgcc
     2281 cgaggtcggc catctggtgc cgacgatgac accggtcgtc gccgccaggg agagttgaaa
     2341 tcccgcactc tggacgagac ccgggtcgat cagcaggagc gccaccacgg cggccgatat
     2401 cacctgccag gtctccatca cgactccgac gaggcggcca gccaagacca ggccggccat
     2461 ggcggaggcg cgaaggacgg aaggctcgaa tcgggtcgcc gcggcaaata cgggcaggcc
     2521 gaacaagcca aggaccgccc tccgcttggg cccgatcccg agtgggccgg ccacgacata
     2581 gagcagaccc aggaagatgg cgacgttcga accgctcacg gctgtgaaat gtgagagacc
     2641 ggcacgcctc atggccgact gatcgacctc atcgacccca ctcgtgtcac ccaccatgaa
     2701 cccggcgagc agagcgcgac caccctcgag cgggcttagg cgatcgatca cccggtcacg
     2761 catggcattc cccagggcga gaagtggaga gccgggcccc gacaccaccc gaaaggtggt
     2821 gacatccacg gtgcctcgat gtgggttgcc cgccatctcc cccgcaccg
//
LOCUS       DASTXO010000095         3674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_521470, whole genome shotgun sequence.
ACCESSION   DASTXO010000095 DASTXO010000000
VERSION     DASTXO010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521470"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VFU96_01890"
     CDS             <1..307
                     /locus_tag="VFU96_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112036.1"
                     /translation="WRPLAVTAGIGAFYGLVLVSTSFYFKGWIGQKAWRAIHFASFGV
                     FVTSLLHGVWAGTDSGSPLMIGLYLGSAIVVFSLVALRIVAETSTRGGPAKEPVRAG"
     gene            335..1609
                     /locus_tag="VFU96_01895"
     CDS             335..1609
                     /locus_tag="VFU96_01895"
                     /inference="COORDINATES: protein motif:HMM:NF019626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavohemoglobin expression-modulating QEGLA
                     motif protein"
                     /protein_id="HEU5112037.1"
                     /translation="MTDTYRDTIKELSDRIVEAQRPIKVLNAINWDDSIREKFFAADF
                     KDLPTIDHAYYEARDTKLDPDATRNELRSIEADLQSKLGPVSPAGNLMKFMCEQFRLT
                     VDLLDATGTPAFSQIASLLYGTPHDVFHVGGPTIADLAQEMRKVLQGLDVQSETEKDL
                     RNIPGTEAVAILQARVDTSMGKGMVDVRPDDGIAADAAAGSDYIKVREDRMFSQRDLH
                     LLETHEAWVHIGTTQNGLSQPVCTFLGKAAPRTTVTQEGLAVLTEMLNLRSHPRRLAK
                     LMHRIEGIRAASEGANFVEVFEMFRTDGLTEDEAWAAAVRIFRGSLPDKGPFTKDLGY
                     GKGLVLTLLYVRLAINLGRSHRIPLLFCGKVDLLDMVTLHQLEEEGLVEEPKFIPPPF
                     DDIAALASTLSLGGFTAMLDWERLEADYSVLI"
     gene            complement(1591..2781)
                     /locus_tag="VFU96_01900"
     CDS             complement(1591..2781)
                     /locus_tag="VFU96_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112038.1"
                     /translation="MRKLETGHAIAISSIIVTPFAALFVLGVAMGGPSKGGVNYLPAA
                     IVWGMVAITWVVAALLLWSSRSASVADVVDMPDLILVSCLGILPRHRREWGDAMRSEL
                     SLVEGKAARWRFAVGCSRAALLARTDSTRVRVTGSVCAVVVAAIWFISGNLTPSMQVF
                     VVALAASIGALITLGAARGQGSSDRQPIHPISVLGVGAVVACVWLTGAFLVQFPESEE
                     QFTTGGAIVLATGLASILWLTLARPKGLMGNRRVMTIGMWAGIILGAGYWVMARTVEA
                     GTIGWPLLAPWLIMFVTGAVASGSDGVRAGVRATVWAAAIGTALMFAVALPEAVLRYR
                     LGGGLLLDGEWAPIGTNLNDAIWVLIAVPILGLPFGVFGATLASRANRRPGPGVGFAQ
                     IRTE"
     gene            complement(2778..3089)
                     /locus_tag="VFU96_01905"
     CDS             complement(2778..3089)
                     /locus_tag="VFU96_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020640696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5112039.1"
                     /translation="MTLTRKPSAQTSAVMLSLAAEPTEWRYGYELCQELGIKAGSMYP
                     ILIRLADRGMLETTWESEMPAGRPPRHLYRLTGTGLALARTLSTTSEGQIGLTPRVQT
                     I"
     gene            complement(3135..>3674)
                     /locus_tag="VFU96_01910"
     CDS             complement(3135..>3674)
                     /locus_tag="VFU96_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112040.1"
                     /translation="QDIDVIVVGSHLAYLHRAPFGGLVGELLRTAPADVLVTVNTAPV
                     LTPGPGPIGVWLPGEPTDLATLDLANSLARGLESTLRVIGVNGAVAGVEADEVIEMGV
                     DVTTAKVVNALEGVSMVVSALPFGHGDLDVEVIETMTTQRRIPVLVLRTASTFALDGR
                     ANRNAVPAGISHDPASQAG"
BASE COUNT          721 a         1189 c         1154 g          610 t
ORIGIN      
        1 ctggcgtcca ctggcggtca ctgccggcat tggcgcgttc tacggcctgg tgttggtcag
       61 caccagcttc tacttcaagg gctggatcgg ccagaaggcg tggcgtgcga tccacttcgc
      121 ctcattcggg gtattcgtca cctccctgct gcacggggtg tgggcgggaa cggacagcgg
      181 gagcccgctg atgatcgggc tgtatctcgg atcggcgata gttgtcttct cactcgttgc
      241 gctgcgcatc gtcgccgaga cctcgacacg gggcggtcca gcgaaggagc ctgtgcgcgc
      301 cggatgactg gtctccccta gattcgccgg tcacatgacc gacacctatc gcgacaccat
      361 caaagagctg agcgaccgga tcgtcgaggc ccaacgaccc atcaaagtcc tcaatgcgat
      421 caactgggac gactcgatca gggaaaagtt cttcgcggcg gacttcaagg acctaccgac
      481 gatcgaccac gcctactacg aagcccgcga caccaagcta gatccggacg cgacccgcaa
      541 cgagctgcgg agcatcgaag ccgaccttca gtccaagctc ggccccgtgt ccccggccgg
      601 caacctgatg aagttcatgt gtgagcagtt caggctgacg gtcgacctgc tcgatgccac
      661 cggaacgccg gctttctccc agatcgcgag tcttctctat gggaccccgc acgatgtctt
      721 tcatgtcggc gggccgacga tcgccgacct ggcacaggag atgagaaagg tcctccaggg
      781 gctggatgtt cagtcagaga cggagaagga cttgcgaaac atccccggga ccgaggccgt
      841 agcgatcctt caagccagag tcgacacctc gatggggaag ggaatggtcg atgtccggcc
      901 ggacgacggg atcgcggcgg acgccgccgc cgggagcgac tacatcaaag tgcgagaaga
      961 ccgcatgttc tcccagcgcg acctccacct cctcgagacc cacgaggcct gggtgcacat
     1021 cgggaccaca cagaatggcc tctcacagcc cgtctgcacc tttctgggca aagctgcccc
     1081 ccgtacgacg gtgacccagg aaggtctggc agtcctcacc gagatgctca acctgcgcag
     1141 tcatccccgt cggctcgcca agctcatgca tcgcatcgaa gggataaggg cggccagcga
     1201 gggggctaat ttcgtcgagg tgttcgagat gtttcgcacc gacggtctca ccgaggacga
     1261 ggcctgggcg gccgcggttc gcatctttcg cggcagcctg cccgacaagg ggcccttcac
     1321 caaagacctg ggctatggca aggggctggt cctgaccctc ctctacgtac ggctggcaat
     1381 aaacctgggt aggtcacatc gaatacccct gcttttctgc gggaaggtgg atctcctcga
     1441 catggtgacg cttcaccaat tggaggagga gggccttgtc gaagagccga aattcatccc
     1501 tccgcccttc gatgacatcg cggccctggc gtcaacattg agcctgggtg ggttcaccgc
     1561 catgctcgat tgggaacgtc tcgaggccga ctactcggtc ctaatctgag cgaaaccgac
     1621 gccgggccct gggcgcctgt tcgcccgact ggcgagcgtc gctccaaaga ccccgaaggg
     1681 aagaccgagg atcgggacgg cgatgagtac ccagatggca tcgttgagat tggtgccgat
     1741 cggggcccat tctccgtcca aaaggaggcc tcctccgagg cggtaacgca gcactgcctc
     1801 gggcagggcc accgcgaaca tgagggcagt tccgatggct gcggcccaaa ccgtcgccct
     1861 gaccccggcc cgaacgccgt cgcttccaga agccacggcc cctgtgacga acatgatcag
     1921 ccacggggca agtagcggcc agccgatagt cccggcttcg accgtgcgag ccatgaccca
     1981 atagccggcg ccgaggatga tgcccgccca catgccgatc gtcatgacgc gccggttccc
     2041 catcagccct ttcggccggg cgagtgtcag ccagaggatc gacgccagac cggtggcgag
     2101 gacgatggcc ccacccgtgg tgaactgctc ttcggactcg gggaactgca ccaagaaagc
     2161 cccggtgagc cagacgcatg ccaccactgc gccaacaccg agcacagaga tcggatggat
     2221 gggctgccgg tcactgctcc cctgacctcg ggcagctccc aacgtgatca gtgcccctat
     2281 cgaggcggcg agagccacaa cgaacacctg catcgacggg gtcaggtttc ctgagatgaa
     2341 ccagatcgcg gccacgacca cagcgcagac cgatccggtg acccggaccc gtgtcgagtc
     2401 cgtgcgcgca agaagagcag cccgggagca gcccaccgcg aatcgccatc gagcagcctt
     2461 cccctcgacc agagagagct cggaccgcat cgcatcgccc cattcgcggc ggtggcgggg
     2521 gaggatgccg agacagctga cgaggatcag gtcgggcatg tccacgacgt cggcgacaga
     2581 cgctgacctc gacgaccaga gcagcagggc ggcaacgacc caggtgatcg cgaccatccc
     2641 ccagacgatg gcggctggga ggtagttgac cccgcctttg gagggccctc ccattgcgac
     2701 acccagcacg aagagggctg cgaacggcgt gacgattatc gaagagatag cgatggcgtg
     2761 accagtctcg agctttctca tatcgtctgg acccgtggcg tcaaaccgat ctggccctct
     2821 gaggtcgtgc tgagagtccg tgccagggcc agcccggtcc cggtcaggcg gtacaggtgg
     2881 cgcggaggcc gtccggccgg catctcgctt tcccaggtgg tctcgagcat cccgcggtcg
     2941 gcgaggcgaa tcaggatggg gtacatcgac cctgctttga tcccgagctc ctggcagagc
     3001 tcgtatccat accgccactc ggtgggctcg gcggccagag aaagcatgac cgctgaggtt
     3061 tgggccgacg gttttcgcgt caaggtcacc atccaactct atatagatag agttaactgg
     3121 tcaagcgagt tccgtcagcc tgcctgactg gccggatcgt gggagatgcc cgccggcacc
     3181 gcgttccggt tcgccctccc gtccagggcg aatgtcgaag cggtgcggag aaccagcacg
     3241 gggatccttc gctgtgtggt catggtttcg atgacctcca catcgaggtc gccgtggcca
     3301 aagggcagcg cggatacaac catggagacc ccctccaggg cattcaccac cttggcggta
     3361 gtgacatcga cccccatctc gatcacctca tctgcctcga ccccggctac agccccgttg
     3421 acgccgatga ctcggagcgt tgattccaaa cctcgggcga gactgttggc gaggtcgagc
     3481 gtggcgaggt cggtgggctc tccaggcagc cacacaccaa tcgggccagg tccgggggtc
     3541 agcaccggag ctgtattgac cgtcaccagg acgtcagcgg gagcagtccg gagcagctct
     3601 ccgacgagcc ctccgaatgg agcacgatga aggtacgcca ggtgggatcc gacgacgatg
     3661 acatcgatgt cctg
//
LOCUS       DASTXO010000096         2285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1073749, whole genome shotgun sequence.
ACCESSION   DASTXO010000096 DASTXO010000000
VERSION     DASTXO010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073749"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VFU96_01915"
     CDS             <1..556
                     /locus_tag="VFU96_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEU5112041.1"
                     /translation="AMLEPVGVALHAIDLAGEVEGASVGVIGCGPIGLLLIAALGHLG
                     LDRIVVTDPLTHRVEAAVTLGATSAGEMVGGLDVVFDTAGSDLALHTALTAARPGGRV
                     VLIGIPDGDRTSYVASLARRKELTLVVCRRMLPGDLVRAAEMAGSGLRGLESLVSHRY
                     PLSQAGDAFDTLVEQVGLKVMIIP"
     gene            719..1747
                     /locus_tag="VFU96_01920"
     CDS             719..1747
                     /locus_tag="VFU96_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU5112042.1"
                     /translation="MTSRPVTMQDIAKAAGVSQSTVSRVLNDTPTSVPIAADTRERVQ
                     EVAKRLGYRPNPLARGLRGARTMLLGVIVREITDPFFSMAIEALSTEALARGYNVVLG
                     SAHSKADEAIALRAVLETRHCDAILLLGDMRDEPKFLEDLRASQTPVIALWHGTELEG
                     VPTVNTDNRAGMVAAVDHLVDKGHDRIAFIGGRPLGDIRERRTAFYEHLEERGIAVPE
                     AYVQNVINEPAGGDAALRTLVTLDTPPTAVVCSTDHLATGVLHAAADLGIVVPRDLSV
                     VGFDDIPMASFTVPSLTTVHMPVAEMTALAARLAMDEPEDDQSGAVTSFVVAPTLVVR
                     ESTGEAPR"
     gene            1747..>2285
                     /locus_tag="VFU96_01925"
     CDS             1747..>2285
                     /locus_tag="VFU96_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEU5112043.1"
                     /translation="MNGDHADLRRLAAKANNEIAEAGLVVHAFGNASQVDREAGVFAI
                     KPSGIPCEEVNADDMVVVGLDDGEVVWGENRPSSDTPTHRAIFIGIDTAAGVVHTHSR
                     FATAWAQARLPIPCLGTTHADHFRGPVPVTRPLSSVEIEGDYEANTGRVIVEMYDAGG
                     LHPEEAPGALVASHGPFVWG"
BASE COUNT          383 a          754 c          762 g          386 t
ORIGIN      
        1 ggcgatgctc gaaccggttg gcgtcgccct ccatgccatc gatctggcgg gcgaagtcga
       61 aggcgcctcc gtcggtgtga tcggctgtgg gccaatcggc ctactcctca tcgccgccct
      121 cggtcatctg gggcttgatc ggatcgtggt caccgacccc ctgacccatc gggtcgaggc
      181 ggcggtcacc ctgggcgcca catccgccgg ggagatggtc ggtgggctcg atgtcgtgtt
      241 cgataccgca gggagcgatc tcgccctcca cactgctctg acggcggccc gtccgggcgg
      301 ccgcgtcgtc ttgatcggca tccccgacgg agaccggacc tcctatgtcg cctcgctggc
      361 ccgtcgcaag gagctgactc tggtggtttg ccgacggatg ctgcccgggg acctggtccg
      421 ggcggccgag atggccggat cgggcctacg cggcctcgaa tctctggtca gtcatcggta
      481 tccgctgagc caggccggag acgcattcga cactcttgtc gaacaggtcg ggctcaaggt
      541 gatgatcatc ccctgatcag gggtacaacc aaatggataa cctatgcagg caaggctatg
      601 ctgacaatcg tatccagcgg tcctcctccg cgggggaagg tggcgcggtt aggtttggcc
      661 gcacgcacgc tcacgggatg ggagagtgcc ggatgcacgt gccgagaggg agtgtgggtt
      721 gacgtctcgc cctgtgacca tgcaggacat cgccaaggca gcgggggtgt cccaatcgac
      781 ggtgtcgcgg gttctcaacg acaccccgac gtcggttccc atcgccgccg acaccaggga
      841 acgagttcag gaggtggcga agcgcctcgg gtatcgcccc aatccattgg cgcgtgggct
      901 ccggggcgca cgcaccatgc tgctcggggt catcgttcgc gagataaccg acccgttctt
      961 ctcgatggcg atcgaggcac tgtcgaccga ggcgctggcg cgcggataca acgtcgtcct
     1021 gggcagcgcc cacagcaagg cggacgaggc gattgccctc agggcggtgc tggagacccg
     1081 tcactgcgac gccatcctcc tcctcggaga catgagggac gagcccaagt tcctggaaga
     1141 cctgcgagct tcacagacgc cggtcatcgc cctctggcat gggaccgaac tggagggcgt
     1201 gcccaccgtc aacacagaca accgtgccgg gatggtggca gcggtcgacc atctcgtcga
     1261 caaaggtcat gaccgcatcg cattcatcgg cggccggccc ctgggcgaca ttcgcgagcg
     1321 acgcaccgcc ttctatgagc atctcgagga gaggggcatc gcggtgcccg aggcatacgt
     1381 tcaaaacgtc atcaacgagc cggcgggtgg ggacgcagct ttgcgcactc tcgtcacctt
     1441 ggatacgccg cctaccgcgg tcgtgtgctc caccgaccat ctggccaccg gggtgctcca
     1501 tgccgcggcc gatctcggga tcgtcgtgcc ccgggatctg tcggtggtcg ggttcgacga
     1561 catcccgatg gccagcttca ccgtgccttc cctgaccacg gtccacatgc cggtagccga
     1621 gatgacggcc ctggccgctc ggctcgccat ggacgagccc gaggacgacc agtccggtgc
     1681 cgtgacgagc tttgtcgtgg ccccgacatt ggtcgttcgg gagtcgaccg gcgaggcacc
     1741 acgctgatga acggcgacca cgccgaccta cgacggttgg cagccaaggc caacaacgag
     1801 atcgccgagg ccgggctggt agtccatgcc ttcggcaacg ccagccaggt cgatcgggag
     1861 gccggcgtct tcgcgatcaa gcccagcggc atcccctgtg aggaggtcaa tgcggacgac
     1921 atggtcgtgg tcggactgga cgacggggag gtggtgtggg gcgagaaccg accctcctcg
     1981 gacacaccaa cacaccgggc catcttcatc ggcatagaca ctgccgcggg cgtggtccac
     2041 acccactcca ggttcgccac ggcatgggcg caagcccggc tgcccatacc ctgtctcggc
     2101 accacgcatg ctgaccactt ccggggcccg gtgccggtga cccgccccct gtcgagcgtt
     2161 gagatcgaag gtgactacga ggcgaacacc ggtcgggtca tcgtcgagat gtacgacgcc
     2221 ggcgggctcc atcccgagga agctccgggc gcattggtcg cctcacacgg tccctttgtc
     2281 tgggg
//
LOCUS       DASTXO010000097         2209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_300542, whole genome shotgun sequence.
ACCESSION   DASTXO010000097 DASTXO010000000
VERSION     DASTXO010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300542"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VFU96_01930"
     CDS             <1..389
                     /locus_tag="VFU96_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEU5112044.1"
                     /translation="VGTLCIGTLLLVIAGFLTVEMKSLLIGEAAVDGDIDAIRGAVEA
                     SPHVVRVIDLKTQHIGPEELLVAGKLEFTPALTAPELAAAIDATEASIRLAVPLAGRI
                     YLEPDVWRDDHVPSPDGPSGSEDEGH"
     gene            420..490
                     /locus_tag="VFU96_01935"
     tRNA            420..490
                     /locus_tag="VFU96_01935"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:452..454,aa:Gly,seq:ccc)
     gene            676..1257
                     /gene="dcd"
                     /locus_tag="VFU96_01940"
     CDS             676..1257
                     /gene="dcd"
                     /locus_tag="VFU96_01940"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012245612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEU5112045.1"
                     /translation="MIFSDRSIREAIESGLIQIDPYESSYVQPSSVDLRVGDGFRVFV
                     NHRYSLIDPRDPQADLTRLVEVGDDEPFMLHPGEFVLGSTLERVKLGNDVVARLEGKS
                     SLGRLGLLIHSTAGFIDPGFEGHITLELSNVATLPIAIYPGMKIGQVSFYQMTTEAEL
                     PYGSPELGSKYQGQSGPTASRSHRDFEGAGPAN"
     gene            complement(1437..2078)
                     /locus_tag="VFU96_01945"
     CDS             complement(1437..2078)
                     /locus_tag="VFU96_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112046.1"
                     /translation="MKVFRASIFFLALVLAACGGDAGGDTTVAETTTTAAEAEGGAGT
                     VDFEDMPQECIDALVGYLRAIEPAVEDVDFDATTGADLEALGTELDSLSEDYTTAIED
                     LDCPDPSGTDEEAFAAIIELAEQEAPGTVGYLEWVQSLAAGFGDTEASGDCETDIAAL
                     QAIIDENASMSDLTMGEVVQVGSLVASVSTACSPERAEEFFAEEDVAAFLESQ"
BASE COUNT          389 a          731 c          656 g          433 t
ORIGIN      
        1 cagttgggac cctgtgcatc ggcaccctgt tgttggtgat cgccggcttc ctcactgttg
       61 agatgaagtc tcttctgatc ggggaggcag ctgtggacgg cgacatcgac gcgattcgag
      121 gggcggtcga ggcgagtccg catgtggtga gggtcatcga tctcaagact cagcacatcg
      181 gccccgaaga gctgctggtc gccggcaagc tcgagttcac cccggcgctc actgcccctg
      241 agctcgccgc ggcgatcgac gccactgagg catccatccg cctcgccgtt cccctggcgg
      301 gtcggatcta tctggagccg gacgtctgga gggatgacca tgtccccagc cccgatggac
      361 cctcgggatc ggaagacgaa gggcactgag cccggccggc gttgtaatct gggccgaacg
      421 cgggtgtagt tcaatggtag aacacgagct tcccaagctc ggagcgaggg ttcgattccc
      481 ttcacccgct ctccggctct cccccgctcg tgtcagaaca cgaggctgtg tccaatggct
      541 cgagcgaggg tttgattccc ttcatccact caacacgagg ctgtgcccaa cggctcgagc
      601 gagggttcga ttcccttcac ccgctcccac cagacttgga accgccgacg ggtgctgcca
      661 tctaccatcc tctcggtgat cttctctgac cgctccatcc gggaggccat cgaatcgggt
      721 ttgatccaga tcgatcccta cgagtcgtcc tacgtccagc catcgtcggt cgatctgcgg
      781 gtcggcgacg ggttccgggt cttcgtcaac caccgttaca gcctcatcga ccctcgtgac
      841 ccccaggccg acctgactcg gttggtcgag gtgggcgacg atgagccttt catgctccat
      901 cccggcgagt tcgtgctggg atcgactctg gagagggtga agctgggcaa cgatgtggtg
      961 gcccggctcg aggggaagtc gtccctgggg cgtctcggcc tgctcatcca ttccacggcc
     1021 ggcttcatcg acccgggatt cgaagggcac atcacactcg agctgtccaa cgtcgcaacc
     1081 ctgcccatcg cgatctatcc ggggatgaag atcggccagg tcagcttcta ccagatgacc
     1141 accgaagcag aactccccta tggatcgccc gagctcggct ccaagtacca gggtcagagc
     1201 gggcccaccg ccagcaggtc tcaccgggat ttcgagggtg ccgggcccgc gaactagcgc
     1261 gactgcgtat agttcgaatt ggatccggtt ctgcccgtcc aagggggctc gggaagtaga
     1321 gaattggatt caggtcccac aaagccctaa ttgagtcccc cctctagaag cttccccggc
     1381 ttcacagggc aatgcgggaa cccctccaag cggcaacctt cgggttgccg ctcgtcctac
     1441 tggctttcga ggaaggctgc gacgtcctct tcggcgaaga actcctccgc ccgctccggg
     1501 ctacaagccg tcgagaccga tgccaccagg cttcctacct gaaccacttc tcccatggtg
     1561 agatcggaca tcgaggcgtt ctcgtcgatg atcgcctgca atgctgcgat gtcggtctca
     1621 cagtcaccgg aagcttctgt atctccgaaa ccggcggcga ggctctgcac ccattccagg
     1681 tagcccactg tgcccggcgc ctcctgctcg gccaactcga tgatcgcggc gaacgcctct
     1741 tcgtcggttc ctgaaggatc agggcagtcg aggtcttcga tggccgtcgt gtaatcctcg
     1801 gatagcgaat ccagctcggt gccaagggct tcgaggtccg cgcctgtggt cgcgtcgaag
     1861 tcgacgtcct cgacagccgg ctcgatcgcc cgcaggtatc cgaccagggc gtcgatgcac
     1921 tcctgaggca tgtcttcgaa gtcgaccgtt cctgccccgc cctccgcctc cgcggctgtg
     1981 gtcgtggtct cggccacggt cgtgtcccct ccagcgtcac cgccacaggc cgccagcacc
     2041 agggccagga agaaaatcga tgctctgaat accttcatgg cgccacctcg actcgagagt
     2101 ccgaatctcg gacgttgcca gcgtaaatgg acgggttcac aacgccactt gcttcccgat
     2161 ctcgatgacc tgaccgggag gaagcgtgaa gaactgaacc gggctcgac
//
LOCUS       DASTXO010000098         2499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_226699, whole genome shotgun sequence.
ACCESSION   DASTXO010000098 DASTXO010000000
VERSION     DASTXO010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226699"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /locus_tag="VFU96_01950"
     CDS             <1..1198
                     /locus_tag="VFU96_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5112047.1"
                     /translation="QTLFKVQLPMAMPFIMTGVNQTIMMALGIVVLATLLGAGGLGQE
                     VLEALNQRRTGRGLAAGLAIVAVAMVLDRVGRSLAFADRTRPASRRYVITALALVAGA
                     IVVGRVMGWVEFPAVWEVRVFDPVDTFVRWGRDNLSFITRPVNDFIVAGLLIPARDFL
                     ILTVAWPVLILVTAVVCWKLKGVGLAIFAGAALLMVGLIGMWELALQTLVQVLAAALI
                     SVAIAIPVGIWAGRSPRVEKVLTPFLDALQTIPSFVFIIPVILLFTVGQVPGIIAAVL
                     YAIVPGIRITALGIKEVPEESVEASETFGATRRQTMFGVRIPLAAPTILAAVNQVIMM
                     VLAMVIIAGLVGGGALGFETVRAVTRGLTGLGFEVGFAIVVMAIILDRLTQAWAARLQ
                     PPTPAH"
     gene            1223..>2499
                     /gene="betB"
                     /locus_tag="VFU96_01955"
     CDS             1223..>2499
                     /gene="betB"
                     /locus_tag="VFU96_01955"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HEU5112048.1"
                     /translation="MRLDTQQLYIGGHRIDADSGETFATINPATGSEICQVQHAGPSD
                     VDRAVASAREGFEVWRQLSGAERGRILNRAAGLLRENNEELARLEVLDTGKPIQEALA
                     VDVLSGADAIEYYAGLAATIHGETFDLGSAWAYTRREPLGVCVGIGAWNYPIQIACWK
                     SAPALACGNSMVFKPAELTPLTALRLAEIYSEAGLPDGVFNVVEGDHRTGEALVSHPD
                     VAKVSLTGEVGTGKIVMANAAATLKHVTLELGGKSPLIIFDDADLDNAVSAALMANFY
                     TQGEICSNGTRVYVHEEIHDEFLERLVTRTSKMKIGDPMDPDTHVGALISREHMEKVL
                     SYIDAGKTAGAKVVIGGERPSDSLLEGGFFVEPTVFDACTDDMSIVKEEIFGPVMAVL
                     TFRDEDDVIARANDTPYGLAAGVFTRDIQRAHRV"
BASE COUNT          409 a          779 c          827 g          484 t
ORIGIN      
        1 acagaccctc ttcaaggtgc agttgccgat ggctatgccc ttcatcatga ccggggtcaa
       61 ccagacgatc atgatggccc tgggcatcgt ggtcctggcc acgctcctcg gcgctggtgg
      121 attgggtcaa gaggtgctcg aggcgttgaa tcaacggcgc accggacggg gtctggctgc
      181 cggcctcgca atcgtggcgg tggcgatggt gctggacaga gtcggacgat cacttgcctt
      241 cgccgacaga acccgacctg cctcgcgacg ctacgtgatc acagcgctgg cgttggtcgc
      301 cggcgcgatc gtcgttggac gggtcatggg ttgggtcgag tttccggcgg tgtgggaggt
      361 cagagtcttc gacccagtcg acacctttgt gcgatggggg cgggacaatc tctccttcat
      421 cacccgcccg gtcaacgact tcatcgtggc cgggctgctc atcccggcgc gtgatttctt
      481 gatcctcact gtcgcctggc cggtcctgat actcgtcacc gccgtcgtct gctggaaact
      541 gaagggggtt ggtctggcga tcttcgccgg agcggccctc ctcatggtcg ggctcatcgg
      601 gatgtgggag cttgccttgc agactctggt ccaggtgctg gcggccgcac tcatatcggt
      661 ggcgatcgcc atcccggtag ggatctgggc cggccgcagc ccgagggtcg agaaggtgct
      721 gaccccattc ctggacgcgc tccaaacgat cccctctttc gtgttcataa tcccggtgat
      781 cctcttgttc acagtcgggc aggtgccggg catcatcgct gccgtcctct acgccatcgt
      841 tcccggtatc cggatcacgg ctctcggcat caaagaggtg ccggaggaga gtgtggaggc
      901 atcggagacg ttcggggcca cgcgacgcca gacgatgttc ggggtgcgaa ttccgcttgc
      961 cgccccgacg atcctggctg cggtcaacca ggtgatcatg atggtgctgg ccatggtgat
     1021 catcgccggc ctggtcgggg gtggggccct tggcttcgag acggttcgcg ccgtgacccg
     1081 cgggctgacc ggcctcggtt tcgaagtcgg tttcgccatt gtggtgatgg ccatcatcct
     1141 cgatcggctc acccaggctt gggcggcgcg gctccaaccg ccgactcctg cccactgaat
     1201 tggccctact accgtctcgc cgatgaggct cgacacccaa cagctctaca tcggcggcca
     1261 ccgtatcgac gccgattcgg gtgagacctt tgccacgatc aacccggcca ccgggtcgga
     1321 gatctgccag gttcagcatg ccgggccgtc cgatgtggac cgggccgtgg cctcggctcg
     1381 ggagggattc gaggtctggc gacagctctc tggggcggag cggggacgga tcctcaaccg
     1441 ggctgccggg ctgctacggg agaacaacga agagctggcc cgtctcgagg tgctggacac
     1501 gggcaagccg atccaggagg cccttgcggt cgacgtcctg tccggcgccg acgccatcga
     1561 gtattacgcg gggctggcgg ccaccatcca cggggagaca ttcgacctgg gcagcgcctg
     1621 ggcctacacc cgtcgtgagc cactcggggt gtgcgtcgga atcggggcgt ggaactaccc
     1681 gatccagatc gcctgttgga agtcagctcc ggccctggcc tgtggcaact ccatggtttt
     1741 caagccggcg gagctcacac cgctgacggc cttgaggctc gccgagatct attccgaggc
     1801 gggtcttcca gatggggtat tcaacgtcgt cgagggcgat caccgcaccg gggaggccct
     1861 cgtgtcccac cccgatgtgg ccaaggtgtc gctgacgggc gaggtgggca ccgggaagat
     1921 cgtcatggcc aatgccgcag ccactctcaa acacgtgacc ttggagctgg ggggcaagag
     1981 cccactgatc atcttcgacg acgccgatct cgacaatgcg gtgtcggctg ccctgatggc
     2041 gaacttctac acccagggcg agatctgttc caacgggacc agggtctacg tccacgagga
     2101 gatccacgac gaattcctcg agcgtctggt cacacgcact tccaagatga agatcggcga
     2161 cccgatggac cccgacactc acgtcggtgc tctgatctcc cgagagcaca tggagaaagt
     2221 cctttcgtat atcgatgcgg gcaagacggc tggagcgaag gtggtcatcg gtggggagcg
     2281 gccctctgac tcgttgctcg agggcggctt cttcgtcgag ccgaccgtgt tcgacgcatg
     2341 caccgatgac atgtccatcg tcaaagagga gatcttcggg ccggtgatgg cggtcctcac
     2401 cttcagggac gaggacgacg tgatcgcccg agccaacgac acgccgtatg gcctcgctgc
     2461 gggcgttttc acgcgcgata tccagcgcgc ccatcgggt
//
LOCUS       DASTXO010000099         3119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1737113, whole genome shotgun sequence.
ACCESSION   DASTXO010000099 DASTXO010000000
VERSION     DASTXO010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1737113"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VFU96_01960"
     CDS             <1..461
                     /locus_tag="VFU96_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018349230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEU5112049.1"
                     /translation="ATAGSGVELDIPIGTMIELPRAALTAGEIAEVADFFSFGTNDLT
                     QMTYGLSRDDAEGLFLRDYLEQGILSSDPFQTLDRAGVGRLVRVATKEGREANEDLVV
                     GLCGEHGGDPESIYFVHSLGLDYVSCSPPRVEGARLAAAQAALQADHTDD"
     gene            complement(463..1173)
                     /gene="meaB"
                     /locus_tag="VFU96_01965"
     CDS             complement(463..1173)
                     /gene="meaB"
                     /locus_tag="VFU96_01965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEU5112050.1"
                     /translation="MSDSDLAGRVRGGDRRSLARLITAIEAGDPVGESAVSSLVDSTA
                     PTRRVGITGPPGAGKSTLVNAMVTELRARGDTVAVLAVDPSSPITGGALLGDRIRMQD
                     HAGDPGVYVRSMASRGHLGGLSAAAAPAIAALARAGFDLVLIETVGVGQSELEVMDHV
                     DLVVLVLGPGWGDQIQADKAGIVEIADVFVVNKGDRPGVDIVQLALLDRLGGSDAPVL
                     ITTAISGEGVPGLVDVIR"
     gene            complement(1196..1534)
                     /locus_tag="VFU96_01970"
     CDS             complement(1196..1534)
                     /locus_tag="VFU96_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEU5112051.1"
                     /translation="MSETTPVTISSSVRPDEGPIDVDKLRETLEYIRPAVQADGGDLV
                     LLGTEDGRVDIQMVGACGGCPLSMMTLKAGIERILMDRVPGVVEVRASADAPGMDDVD
                     SPNAITIPAL"
     gene            complement(1592..2884)
                     /locus_tag="VFU96_01975"
     CDS             complement(1592..2884)
                     /locus_tag="VFU96_01975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEU5112052.1"
                     /translation="MIARRALAVAAMLIMMAPPALAVRPEGSVVEVSAPEGSYLELDG
                     RRYRGPLRVTAESDGTAVVEVTSLDAYLEGIREVPFSWHEEALAAQVVAARTYLAWTL
                     QGGRTETGSRIGYDICATAACQVYAGVDAVLGEDGDRWRQAVRRTSGEILVYQGEPAR
                     AFYSSTTGLRTRNIEDIWPGSEPAPYLVGVPSPGEDSPFVEWSWELPGYQMEQLLREA
                     EVADGDVRSITTRTTEDGQGPWMIDIVSDSGTVSMDTWALRSRLNEAAAILPGLLPAK
                     RPDGIDYPTTVLSPEYSIRGEVRVSHVGGDALAIAVPYYIVEGAGWGHLIGMSQYGAQ
                     AMAGAGSAYPEILAHYYGGLTPVAGDRFLPDQLAVGLVIGSDSVSIRSESPLTVLVDG
                     TPVETTQTGSWGFIWVDGALQVTQPVPYVPPSGQPDEF"
     gene            complement(2881..>3119)
                     /locus_tag="VFU96_01980"
     CDS             complement(2881..>3119)
                     /locus_tag="VFU96_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012785442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU5112053.1"
                     /translation="ARMGADITTEAQHAIIRGVERLSGCPVLAPDIRAGAALVLAGLR
                     AEGRTVVGEASHIDRGYEEFPERLRSLGARIERA"
BASE COUNT          535 a         1054 c         1016 g          514 t
ORIGIN      
        1 cagcgaccgc gggctcgggg gtcgaattgg atatcccgat cgggacgatg atcgagcttc
       61 ccagggccgc cctcaccgcg ggcgagatcg ccgaggtggc cgacttcttc tcatttggca
      121 ccaacgattt gacccagatg acctacggcc tgagccgtga cgacgccgag ggtctgttcc
      181 tgagggacta cctcgagcag ggcatcctca gctcggatcc tttccagaca ttggatcggg
      241 ccggtgtagg acgcctggtc cgggttgcga ccaaggaggg acgggaggca aacgaagacc
      301 tggtcgttgg gctgtgtggc gagcacggtg gcgacccaga gtccatctac ttcgtccact
      361 ctctgggtct cgactacgtc tcgtgcagcc ctccgcgcgt ggagggagct cggctcgccg
      421 cagcgcaggc cgcattgcag gccgatcaca ccgacgactg agtcagcgga tcacgtcgac
      481 caggccgggc acaccttccc cggagatcgc ggtcgtgatc aagaccggag catcggagcc
      541 accgagtctg tcgagaaggg cgagttgcac gatgtccacc ccgggccgat cgcccttgtt
      601 gaccacgaag acgtcggcaa tctcgacgat cccagccttg tcggcctgga tctggtctcc
      661 ccagccggga ccgaggacca ggacgacgag atcgacgtga tccatcacct ccaattccga
      721 ctggcctact cccaccgtct cgatcaggac caggtcgaag cctgctcgcg ccagggcggc
      781 gatggctggg gctgcggcag ccgacaggcc accgaggtgg ccccgggacg ccatggaacg
      841 gacgtatacc cccgggtcgc cggcgtggtc ctgcatgcgg atccggtcac cgaggagtgc
      901 cccgcctgtg atcgggctgg aaggatcgac cgcgagcacg gccaccgtgt ccccccgcgc
      961 cctcaactcg gtgaccatgg cgttgaccag agtcgatttc ccggcgcccg gcggcccggt
     1021 gatgccgacc ctcctggtgg gggcggtgga gtcgacgaga gagctgaccg cggactcacc
     1081 aaccggatcc ccggcttcga tcgcggtgat cagccgagcc aggcttcggc gatcgccacc
     1141 gcggacgcgc ccggccagat cggagtcgct catcgggcga caggttaaga ccgggtcaga
     1201 gagcggggat ggtgatggcg ttggggctgt cgacgtcgtc catgccggga gcatcggcgc
     1261 tggcgcggac ctcgacgact ccggggacgc gatccatcag gattcgctcg atacctgctt
     1321 tcaaggtcat catcgacagt gggcagccgc cgcaggcgcc aaccatctgg atgtcgaccc
     1381 gaccatcttc ggtgccgagc agcaccaggt cgcccccgtc ggcctgaaca gcaggcctga
     1441 tgtactcgag ggtctcacgc agcttgtcga cgtcgatcgg gccctcgtcg ggcctgacgc
     1501 tgctggaaat ggtcacgggt gttgtctctg acatcgcctt gaagaaccct tgcctggtgg
     1561 ggattgttcc gattgtacct ctgcgagcga ctcagaactc gtcgggctgg ccgcttgggg
     1621 gcacataggg caccggctgt gtgacctgga gggccccatc gacccagatg aacccccacg
     1681 acccggtttg ggtggtctcg actggtgtcc cgtcgaccag gactgtgagc ggggattcgg
     1741 accggatgct caccgagtcg gatccaatca caaggccgac agcgagctgg tcgggaagga
     1801 accggtcgcc ggcgactggg gtgagccccc cgtagtaatg ggcaagtatc tcgggatacg
     1861 ccgagccggc cccggccatg gcctgggcgc cgtactggga catcccgatg agatgccccc
     1921 agcccgcccc ttccacgatg taatagggca cggcaatggc cagcgcatct cccccgacat
     1981 ggctgacccg gacctcaccg cgaatcgagt actcgggact cagaaccgtg gtgggatagt
     2041 cgatcccgtc cggtctcttc gccgggagga ggcccggcag gatggcggcg gcctcgttca
     2101 gccgggatcg aagcgcccag gtatccatcg agacggtgcc tgagtcggaa acgatgtcga
     2161 tcatccatgg gccctgcccg tcctcggtgg tccgcgtcgt gatcgaccgg acgtcgccat
     2221 cggccacttc tgcctctctg agcaactgct ccatttgata gcccggcagc tcccatgacc
     2281 attcgacgaa agggctgtcc tcgccgggtg agggaacacc aaccaggtat ggcgccggct
     2341 cggagccggg ccagatgtcc tcgatgttcc tggtgcgaag acctgtggtc gacgagtaga
     2401 aggcgcgcgc cggctccccc tgatagacga ggatctctcc cgaagtccgg cgcactgcct
     2461 gacgccagcg atctccatcc tccccgagca cagcgtcgac gccggcatag acctggcagg
     2521 cggctgtggc gcagatgtca tacccgattc ggctgcccgt ctcggtgcgc ccaccctgaa
     2581 gggtccaggc gagataggtt cgggcggcca ccacctgggc ggccagcgct tcctcatgcc
     2641 agctgaaggg cacctcccga atgccctcca gataggcgtc gagcgacgtg acctcgacta
     2701 ccgccgtccc atctgactcg gcagtgaccc gcaatggccc ccgatagcgg cgcccgtcca
     2761 gctcgagata cgagccttcc ggtgccgata cctcgacgac cgagccctcg ggccggaccg
     2821 ccagcgcagg cggcgccatc atgatcagca ttgcagcgac ggcaagggcc cggcgagcga
     2881 tcacgctctc tctattctcg ctcccagcga tcggagcctc tccggaaact cctcatagcc
     2941 ccggtcgata tgactcgcct cacccaccac ggtcctgccc tcggcacgca gaccggcgag
     3001 cacgagcgcg gctccggcgc ggatgtcagg ggcgagtacc ggacagccgg agagccgttc
     3061 gaccccccgg atgatggcgt gctgggcctc ggtggtgata tctgccccca tccgtgcca
//
LOCUS       DASTXO010000100         3605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_521776, whole genome shotgun sequence.
ACCESSION   DASTXO010000100 DASTXO010000000
VERSION     DASTXO010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521776"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2267)
                     /locus_tag="VFU96_01985"
     CDS             complement(<1..2267)
                     /locus_tag="VFU96_01985"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEU5112054.1"
                     /translation="MVTLAAEGGYQEFTLGPAEWAWLWVAAATAVLALLVGWALSRGV
                     LKADQGTPAMKEIAKAIQEGAMAYLSRQFKTIALILIPLALIVFFTSTAVLHPDGSEA
                     LTFVQSGGFRTLAFIGGCFMSGLTGFIGMSLAVRGNVRTAAAARSGSLPAALKVAFRT
                     GGVAGMFTVGLGLLGATIIIMIFQNTSSAILIGFGFGGSLLALFLRVGGGIFTKAADV
                     GADLVGKIEQGIPEDDPRNPATIADNVGDNVGDCAGMAADLFESYEVTLVASIILGVA
                     AFNSIGANPALGLVFPVVIRAIGVLASIVGVFQVRATDRDKSAMAPINRGFLTAGLLT
                     VVGTLAVAVLYVGNENGNEGWKVFGAVVAGLVLAQVVSRLTEYFTSTESKPVQEIAEA
                     TQTGPATTVLSGISSGLESSVWAIVAIAGALGVAIVLGGGNIQFSLYLVALTGMGMLA
                     TTGVVVSEDTFGPVADNAAGIAEMSGEFHGEARKVMVGLDAVGNTTKAVTKGFAIGSA
                     VVAAVALFASFIETIGGELGLVAEGSDLFTLPETSINVADPTAFIGLLIGGSIAFMFS
                     GLAIRAVGRTAAVVVQEVRRQFADGAIMRGDRRPDYGPVIDICTAASLRELLTPALLA
                     VLTPVIVGFGINYFALGAFLAGSILTGQLMANFLSNSGGAWDNAKKYIEDGHYGGKGS
                     ESHKAAVIGDTVGDPFKDTAGPALNPLIKVMNLISLLILPAVILLQDSSARYWIAGIS
                     LVILLSAITWSKLQP"
     gene            2393..3025
                     /locus_tag="VFU96_01990"
     CDS             2393..3025
                     /locus_tag="VFU96_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017594930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEU5112055.1"
                     /translation="MLDGLVEWVTSVVESLGYGGVAFLVALENLFPPIPSEVVLPLAG
                     FVAANGEASLVGMIVAATIGSMIGAFLLYGISAAIGPVRLRALVMRYGRWFRLDESDL
                     DRTEEWFDKRANVAVLLCRCVPLMRSLISIPAGFRRMSIVPFTFYTLIGSLVWNTVLI
                     GAGYLLGERWGQVEEPLELFKNAVLVGIGVAVAWYVWRRVVRPRVRGASG"
     gene            complement(3027..>3605)
                     /locus_tag="VFU96_01995"
     CDS             complement(3027..>3605)
                     /locus_tag="VFU96_01995"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5112056.1"
                     /translation="TLLRAVAGLVPVESGSIAWDGEDITKLPTHLRGFGLMFQGYALF
                     PHLDVFGNVGFGLSMAGVPDQEVERRVRAALDLVGLDGFDHRSIADLSGGEKQRVALA
                     RTLAPEPRMIMLDEPLGALDRTLRERLMVDTRRILSDRGVTALVVTHDREEAEALSDR
                     IALMREGSIVQTGTLADILAHPVDEWVETFLG"
BASE COUNT          661 a         1129 c         1193 g          622 t
ORIGIN      
        1 ctcggctgga gcttggacca ggtaatcgcg gagaggagga tgacgaggct gatcccggcg
       61 atccaataac gggccgagga gtcctgtagc aggatgaccg cgggcaggat cagcagcgag
      121 atcaggttca tcaccttgat cagggggttg agcgccggtc ccgcggtgtc cttgaaagga
      181 tccccgacag tgtccccgat gaccgcagcc ttgtgtgact cggagccctt cccgccgtaa
      241 tggccgtcct cgatgtactt ctttgcgttg tcccatgccc caccggagtt ggagaggaag
      301 ttcgccatga gctgtccggt gaggatggag ccggcgagga aggcgccaag ggcgaagtag
      361 ttgatcccga agccgacgat gacgggcgtc agcaccgcga ggagggccgg tgtgagcagc
      421 tcacgcaggg atgccgcggt gcagatgtcg ataacaggtc cgtagtcggg gcgccgatcg
      481 ccccgcatga tggcgccgtc ggcaaattgc ctgcgcactt cctgcaccac caccgccgcc
      541 gtgcgcccca ctgccctgat ggccagaccg gagaacatga aggcgatcga cccaccaatg
      601 aggaggccga tgaacgccgt cgggtcggca acgttgatcg aggtctccgg tagcgtgaac
      661 aggtccgacc cctcagcgac gaggccgagc tcacctccga tcgtctcgat gaacgatgcg
      721 aacagcgcta ctgcagcgac caccgcggag ccgatggcaa accccttggt gaccgccttg
      781 gtggtgttgc ccaccgcgtc gagccccacc atcactttgc gcgcctcgcc atgaaactcc
      841 ccggacatct cggcgatccc ggcggcgttg tcggcaactg gtccaaaggt gtcctcggag
      901 accacgacac cggttgtggc cagcatcccc atcccggtca gggcgaccag gtagagcgag
      961 aactggatgt tgcccccgcc gaggacgatg gcgacgccta gggccccggc gatggcgacg
     1021 atggcccaca ccgatgactc gagacccgat gagatccccg acagcactgt ggtcgccggc
     1081 ccggtctggg tcgcctccgc gatctcttgc accggcttcg actcggtcga ggtgaagtac
     1141 tcggtgagac ggctgaccac ctgggcgagg acgagcccgg cgactaccgc cccgaacacc
     1201 ttccagccct cgttcccgtt ctcgttgccc acatagagga cggcgacggc gagggtgccg
     1261 acgacagtga ggaggcccgc ggtgaggaag ccccgattta tcggcgccat ggccgacttg
     1321 tcacgatcgg tagctcggac ctggaacacg ccgacgatcg aggccaggac gccgatagcg
     1381 cgtatcacga ccgggaatac cagcccgagg gccgggttgg ccccgatcga gttgaacgcc
     1441 gccacgccga ggatgatcga ggcgaccaga gtgacctcgt agctctcgaa gaggtcggca
     1501 gccatgccgg cgcagtcacc gacgttgtca ccgacgttgt cggcgatcgt ggcggggttg
     1561 cgcgggtcgt cctcggggat cccttgctcg atcttgccca cgaggtcggc gccaacgtcg
     1621 gcggccttgg tgaatatgcc accgcccacc cggaggaaca gggcaagtag cgagccaccg
     1681 aatccgaatc cgatcaagat cgccgaggag gtgttctgaa agatcatgat gatgatcgtg
     1741 gccccgagca gaccgaggcc aacggtgaac atgccggcca cgccaccggt ccggaatgcg
     1801 actttgaggg cagccggcag cgacccggat cgggccgccg cagccgtgcg cacgttgccc
     1861 cggaccgcga gcgacatgcc gatgaatccg gtcaacccgg acatgaagca cccgccgatg
     1921 aaagccagcg ttcggaagcc gcccgactgg acgaaggtga gtgcctcgga gccgtccgga
     1981 tggagaacgg cggtcgaggt gaagaagacg atgagggcaa gtgggatgag gatgagggcg
     2041 atcgtcttga actggcgact gagataggcc atcgcccctt cctggatcgc tttggcgatc
     2101 tccttcatcg ctggggtgcc ctgatcggcc ttgagcacgc cgcgggaaag cgcccatccg
     2161 acgaggagag cgagcacagc agttgccgcc gccacccaca gccaggccca ctcggccggg
     2221 ccgagagtga attcctggta accgccttcg gcagccaaag tcaccatgtt caagggaaag
     2281 atcctcctag gggattgcgg gccgagactg tagtggaggc caattcctgt cgacaatcag
     2341 cgcaacggtc gggtctaccg ttgtatcacc ggtcacgagg aaggaatcgc aggtgctcga
     2401 tggtctggtc gaatgggtga cctcggtggt cgaatccctc ggctacgggg gtgtcgcctt
     2461 cctggtggcc ctggagaacc tgttcccgcc gataccctca gaagtggtgc tcccgctggc
     2521 cggcttcgtg gctgcgaacg gcgaggcatc gctggtgggg atgatcgtcg ccgccacgat
     2581 cgggtcgatg atcggcgctt ttctcctcta cggcatctcg gctgccatcg gcccggtgcg
     2641 gctcagggca ctcgtcatgc gttacggccg ctggttccgg ctcgacgagt cggatctcga
     2701 tcggaccgag gaatggttcg acaagcgggc gaatgtcgct gtgcttctct gccgctgtgt
     2761 gcccttgatg cggtcgctca tctcgatccc ggccggtttc agaaggatgt cgatcgtccc
     2821 cttcaccttc tacacactga tcgggagtct cgtttggaac acggttctca tcggggcggg
     2881 gtatctgctc ggggagcgct ggggccaggt cgaggagccg ttggagttgt tcaagaacgc
     2941 ggtcctggtc ggcattggtg tggctgttgc ctggtatgtc tggcgtcgcg tggtcaggcc
     3001 ccgggttcgg ggcgcttcgg gttgattcag cccaggaagg tctccaccca ttcgtcgacc
     3061 gggtgagcga ggatgtcggc gagggtgccg gtctggacga tggacccctc ccgcatcaag
     3121 gcgatccgat ctgagagcgc ctctgcctcc tcccgatcat gggtcaccac cagggcggtg
     3181 actcctcgat cggagagaat tcggcgggtg tcgaccatca aacgttcccg gagagtcctg
     3241 tccaatgcgc ccagcggttc gtcgagcatg atcatcctcg gctccggtgc gagggttcga
     3301 gccagcgcca cccgctgttt ctctccaccc gacagatctg cgatcgatcg gtgatcgaag
     3361 ccgtccaagc cgaccaaatc gagcgcagcg cgcacacgtc tttccacctc ctggtcaggc
     3421 acgccggcca tgcttagacc gaagccgaca ttcccgaaga catcgaggtg cgggaagagg
     3481 gcatacccct ggaacatgag cccgaagccc cggagatggg ttggcaactt cgtgatgtcc
     3541 tctccgtccc aggcgatgga gcctgattcc accggcacca gaccggccac ggcgcgaagc
     3601 aaggt
//
LOCUS       DASTXO010000101         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_374300, whole genome shotgun sequence.
ACCESSION   DASTXO010000101 DASTXO010000000
VERSION     DASTXO010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374300"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            369..947
                     /locus_tag="VFU96_02000"
     CDS             369..947
                     /locus_tag="VFU96_02000"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEU5112057.1"
                     /translation="METGPTIHTERLVLRRWSIGDRAELAALNADPKVMEHFPRILSA
                     EESQQMLQRIELHFEEFGFGLWAVNTIWVPRLIGFCGLAVPTFRTHFTPAVEIGWRFA
                     REEWGNGYATEAGKAALDFGFEQAGLDEILSWTIPANQRSRGVMTRLGMQRAPELDFD
                     HPRFLDDERLRRHVVYRMTREDWSGSGEPDPG"
     gene            995..2026
                     /locus_tag="VFU96_02005"
     CDS             995..2026
                     /locus_tag="VFU96_02005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5112058.1"
                     /translation="MSSHIESVDTRTAPDETLAARHELHLLQDEEVLPGDPPIPLQQR
                     VLDWRHQLETESIYHWVMWKGPEIVATSGFHVDLVQNLKNAYGWVYVHPQHRASGYGR
                     TVAAPMFEAAEADGRTRFAIGINDGRSEEALAERAGMKSAYREKVSRLVFDEIDWTLM
                     ESWMARAAERSSDYELLFLPSPIEDAHVQAYCDIMFVMNSAPQEDYVEDEEEIMTPEI
                     WRDIESKIGVEAGREILTYVARHKPTGKFAGFTTVAHKRLQPDLVEQWDTGVASDHRN
                     RGIGRWVKAAMAFKLRELYPNIRRIDTENAGSNAPMLSINVEMGFRPILIQNVWQGDL
                     ATIRERLSV"
     gene            complement(2023..>2466)
                     /locus_tag="VFU96_02010"
     CDS             complement(2023..>2466)
                     /locus_tag="VFU96_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEU5112059.1"
                     /translation="DLERLMSVEAAYLIEGHIGEDPGLYAPEMSRRARGVPTWAVLKS
                     LGRSGLAEMIDRSVDMARRFADGIREAGFSVLNDVVLNQVLVDFGDPEETLRVVQKLQ
                     ADGIMFAGPTVWQGHTAMRISVSNYGTTETEIDESIAAVVKAANS"
BASE COUNT          489 a          737 c          786 g          454 t
ORIGIN      
        1 ggcatgcgcc gggagtctcg aactgatccg gaccgtcgac ggccagccgc ttgcggtcgg
       61 gaggcgaagg cgggtgatac ccaaccgtct ccgccgtttc attctccatc gggatggcgg
      121 atgctccggc gacggctgtc agagtcgata tcgactcgag gctcaccacg tgatcccgtg
      181 gtcggaaggc gggccgacca ccccggaaaa tctggtgacg ctttgctggt ttcaccacca
      241 agtcgtcgtc catggttttg gataccggat cgacccacgc ctcggaacgg gccgagtccg
      301 cttcatcaag cctggacccg atccaccctg aggtcgcgct cgaatcagcc ttgcctacgc
      361 tgaaagagat ggaaaccggg cccacgatcc acaccgaacg gctggttctc cgacggtgga
      421 gcatcggtga ccgagccgag ttggcggcgc tcaatgccga cccgaaggtg atggagcatt
      481 ttcccaggat cctgagcgct gaggaatcgc aacagatgtt gcagagaatc gagctccatt
      541 tcgaagaatt cggttttggg ttgtgggcgg tcaacacgat ctgggtgccg agactgatcg
      601 ggttctgcgg cctcgccgtt ccaacgttcc gcacacattt caccccggcg gttgagatcg
      661 ggtggcggtt tgctcgggag gagtggggaa acgggtatgc gaccgaggcg ggcaaggcag
      721 cactcgattt cggcttcgag caggccggcc tcgacgagat cctcagttgg acgatcccgg
      781 ccaatcagcg atctcgagga gtgatgacgc gcctgggtat gcagcgggcg cccgagctgg
      841 acttcgatca tccgcgtttc ctcgatgacg aacggctgcg gcgtcacgtc gtctatcgga
      901 tgacccgtga ggattggtcc gggagcggag aaccagatcc gggctgagta attcacccgc
      961 acccggattg ggtggttcct agcctcgacg agtcatgtct tcccacatcg aatccgtcga
     1021 cacgcggacg gcacctgatg agaccttggc agcccggcac gagctccacc tcctgcaaga
     1081 cgaggaggtg cttcccggtg atcctcccat cccgctgcag cagcgggtgc tggattggcg
     1141 tcaccaactg gaaaccgaga gcatctacca ctgggtcatg tggaagggcc cggagatcgt
     1201 ggccacatca ggcttccacg tcgatctggt acagaacctg aagaacgcct acgggtgggt
     1261 ctatgtgcat ccccagcatc gggcgtccgg gtacggccga acagtggcgg ctccgatgtt
     1321 cgaagcggct gaggccgatg gccgaacgcg gtttgcgatc ggcatcaatg acgggcgatc
     1381 cgaagaggcg ctcgccgaaa gggccggaat gaagtcggca tatcgggaga aggtgagccg
     1441 gctggttttc gacgagatcg attggacttt gatggagtcc tggatggcga gggcggccga
     1501 gcgctcttcc gactacgagt tgctgtttct gcccagcccg atcgaggacg cgcacgtaca
     1561 ggcgtattgc gacatcatgt tcgtaatgaa ctcggctcca caggaggact acgtcgagga
     1621 cgaagaggag atcatgaccc cggagatctg gcgtgacatc gagtcgaaga tcggtgtcga
     1681 ggccggaaga gagatcctca cctatgtcgc ccggcacaag ccgaccggaa aattcgccgg
     1741 gttcaccacg gtggctcaca agcggctgca gcccgatctc gtggagcagt gggacacggg
     1801 agtcgcctcg gatcaccgca acagggggat tgggcgatgg gtcaaggcag ccatggcatt
     1861 caagctgcgg gagctctatc cgaacatccg acggatcgac accgagaatg caggctccaa
     1921 cgcgccaatg ctcagcatca atgtcgagat ggggttcagg ccaatcctga tccagaatgt
     1981 ctggcaggga gatctggcca cgatccgaga gaggttgtcg gtctaggagt tcgcggcctt
     2041 caccacggcg gcgatgctct catcgatctc ggtctccgtg gtgccgtaat tcgagaccga
     2101 gatccgcatc gccgtgtgac cttgccacac ggtgggaccg gcgaacatga tcccgtcggc
     2161 ctgcaacttc tgcaccacgc gcagggtctc ctcggggtcc ccgaaatcca cgagcacttg
     2221 attcaggacc acatcgttga ggaccgagaa accggcttcc ctgatcccgt cggcgaagcg
     2281 tcgtgccatg tccacagacc gatcgatcat ttcggccaat cccgatcggc ccagcgactt
     2341 caggaccgcc caggtgggca cccctcgtgc gcgacgagac atctccggcg catacagccc
     2401 cgggtcttcg ccgatgtgcc cttcgatcag gtaggcggct tccacgctca tcaaacgctc
     2461 gagatc
//
LOCUS       DASTXO010000102         4128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417 k141_6149,
            whole genome shotgun sequence.
ACCESSION   DASTXO010000102 DASTXO010000000
VERSION     DASTXO010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6149"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VFU96_02015"
     CDS             complement(<1..666)
                     /locus_tag="VFU96_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU5112060.1"
                     /translation="MAEQPVSEQALIERYGPHLNRVPPGGWDADVEADRVVDTHCCFC
                     GQQCGIRLKVSGDQVIGFEPRYDFPFNEGKLCPKGVKRYLQGNHPDRLLQPLQRDPAS
                     PTGFGVVTWDQALDRTANEIRRIQDRYGADSFAVLSGVSISNEKSYLMGKFARLGIGT
                     ANLDYNGRLCMVSAGAAGKKALGIDRAANPWSDIPLAEVVFVAGANIAECAPITTSYI
                     WRAR"
     gene            complement(666..1715)
                     /locus_tag="VFU96_02020"
     CDS             complement(666..1715)
                     /locus_tag="VFU96_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5112061.1"
                     /translation="MDEPHVSIRLDRRGVGGGAIAVLALLVLVYVGSEGLRWFDAALA
                     PYLFGTLLAVFATVYRYTVWLRRPPTARLNRRGWEALRASGKLGSNLVSLAGMFVTNL
                     ALQRFIANRSRTRWVAHQLLFWGCVLAALVTFPLTFGWVHFESADPSGRTYLAYLGPV
                     ATITFDSRSLFGWLVFHLLNIAAVLVIAGVAIFLVRRLRDRGALATERGGDFAALAGL
                     FAVSVTGLMLTASSMWMEGRFYSFLNTLHALTVILGLLYIPFGKLFHILQRPANLGVA
                     FYKQAGAESGPQTCVECGDGFASTLQMQDLKEVLPQLGFDYSTRGGPNYQDVCPRCRR
                     RLVALAQTDRVGGFG"
     gene            complement(1763..2452)
                     /locus_tag="VFU96_02025"
     CDS             complement(1763..2452)
                     /locus_tag="VFU96_02025"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU5112062.1"
                     /translation="MSRSALASGLAGQLELRSGLISQGHRAIGWKAGFGAPLWRERFR
                     IDGPLVGFMTDATLVDDGAEIDISEFARPVAEPELAAYLGSDLPPGSGREETRAAISA
                     IGPAIELADIDPPPDDVHEILVGNIFHRGVVLGRSDPSRAGADLVGLEARVRLDRREQ
                     ARTNRLEDLTGGVIEVISHLSDLLADHGEMMRAGDVVICGSVVPPIDLFPGSTVEFEL
                     HPMEMISLRAL"
     gene            complement(2449..2844)
                     /locus_tag="VFU96_02030"
     CDS             complement(2449..2844)
                     /locus_tag="VFU96_02030"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5112063.1"
                     /translation="MTDRFRINRHDVPIEGGLREDEGWVDMRVQFLVDAAQAGSDDLL
                     LGWTVLPPGARHDRHRHPNADEFLIVLSGSGMIYTDDEDQRAGEGDVLFTRRGLWHGF
                     DNTGDQDVTLVWGWSGAGSLDAAGYELPE"
     gene            complement(2841..3722)
                     /locus_tag="VFU96_02035"
     CDS             complement(2841..3722)
                     /locus_tag="VFU96_02035"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5112064.1"
                     /translation="MRVGFIGLGDMGQGIVPRLLAAGHEVTGWNRTPGKASPLRDLGM
                     RWADTPRQVAETADVVLSIVTDAAAVESVAVGEDGVLSGLGSDGVYADMSTIDPDASR
                     RIAALFADAGRTMLDAPLSGSPVSLDQGKASVMVGGDRAAFEKVLPVLEAIGPTVTYI
                     GPSGMAVQMKVAINLCLIVEMVAFSEAVALAEKGGVDRVVAVDAMLKSVVASPVMGYR
                     GPFILEGNMPDKPLADVTLQQKDMLLALDLGRRQGSPVPLAAAANEMLNACHGLGLSH
                     RDFVTVFDVYRRLGGDG"
     gene            3813..>4128
                     /locus_tag="VFU96_02040"
     CDS             3813..>4128
                     /locus_tag="VFU96_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5112065.1"
                     /translation="MQFGVFSVGDVTTDPTTGRTPTEAERIKAMVAVALKTEEIGLDV
                     FATGEHHNPPFVPSSPTTMLGYLAARTERIILSTATTLITTNDPVKIAEDFAMLQHLA
                     DGR"
BASE COUNT          777 a         1352 c         1325 g          674 t
ORIGIN      
        1 gcgtgccctc cagatgtagc tggtggtgat cggggcgcac tcggcgatgt tggcccccgc
       61 gacgaacacc acctcggcca gagggatatc ggaccacggg ttggcggccc ggtcgatgcc
      121 gagcgctttc ttgccggcgg cgcctgccga gaccatgcag agtcggccgt tgtagtcgag
      181 gttggctgtg ccgatgccga gccgggcgaa cttgcccatc aggtaggact tctcattgga
      241 tatggacacc ccggagagca cggcgaagga gtccgccccg taacggtcct ggatgcggcg
      301 aatctcgttt gctgttcggt cgagggcctg gtcccaggta accaccccga acccggtcgg
      361 ggaggcggga tcccgttgta ggggttgcag cagcctgtcg gggtggtttc cctggagata
      421 gcgcttcaca cccttggggc agagcttgcc ctcgttgaac gggaagtcgt atctgggctc
      481 gaatccgatc acctggtcgc cactcacctt gagcctgatc ccacactgct gtccgcagaa
      541 gcagcagtgg gtgtccacga cccgatccgc ctcgacgtcg gcgtcccacc ccccgggtgg
      601 gacccggttc aggtgagggc cataacgctc gatcagagcc tgttcgctga ccggctgctc
      661 agccatcagc cgaagccgcc aacccggtca gtctgtgcca gggcgaccag tcggcgccga
      721 cagcgggggc aaacatcctg atagttaggc ccgcccctgg tcgagtagtc gaagccaagc
      781 tggggcagca cttctttgag gtcttgcatc tgcagtgtcg aggcgaaccc gtcaccgcat
      841 tcgacacagg tctgtggccc actttctgct cctgcctgct tgtagaaggc aactccgaga
      901 ttcgccgggc gctgcaagat gtgaaacagc ttcccgaacg ggatgtagag caaaccgagg
      961 atgacggtca gggcgtggag ggtgttgagg aatgaataga agcggccctc catccacatg
     1021 ctggaggccg tgagcatgag accggtcacc gagacggcga acagcccggc aagggcagcg
     1081 aagtcacccc cgcgttcggt ggccagggct ccccgatcgc ggaggcggcg gacgaggaat
     1141 atggccacac cggcgatgac caggaccgcg gcgatgttga gaaggtggaa cacgagccac
     1201 ccgaagaggc tccgggagtc gaaggtgatg gtggcgacgg gccctagata ggccagatag
     1261 gtgcggccgg aagggtcggc agactcgaaa tggacccacc cgaaggtgag cgggaatgtg
     1321 accagtgccg cgagcacaca tccccagaac aggagctggt gggcgaccca cctggtgcgt
     1381 gatctgttgg cgatgaagcg ctgcaaagcg agattggtga cgaacatccc cgccaacgac
     1441 acgaggttcg accccagctt gccgctggcg cggagtgcct cccagccgcg gcgattgagc
     1501 cgggccgttg gcggccggcg cagccacacg gtgtaccggt acaccgtggc gaacacagcc
     1561 agaagcgtcc cgaagagata cggagccagg gccgcgtcaa accatcggag cccctccgag
     1621 ccgacgtaga cgaggacgag cagagccagg acggcgatag cgccaccccc cacacctcgt
     1681 cgatccaggc ggatcgagac atgcggctcg tccatgtgat cgaccctagc cggaaggctg
     1741 ctggcccacc gaccataacc tgttacaagg cgcgcaagga gatcatctcc atggggtgca
     1801 gctcgaattc gaccgtcgat cccgggaaga gatctatcgg gggcaccact gagccgcaga
     1861 tgaccacatc gcccgcccgc atcatctcac cgtgatccgc aaggaggtct gagagatgag
     1921 agatcacctc gatcacaccg ccggtgaggt cttccagacg gttggtccgg gcttgctccc
     1981 ggcgatccaa cctcactcgc gcctccagtc cgacgagatc ggcgccggcg cgactcgggt
     2041 cggatcggcc gaggacgact ccccgatgga agatgttccc caccaggatc tcgtggacat
     2101 cgtcgggcgg tggatcgatg tcggccagct cgatggcagg cccgatggcc gatatcgcag
     2161 ctcgggtctc ctcgcggccg ctgcctggag gaaggtcaga ccccagatat gccgcgagct
     2221 ctggctcggc caccggccgg gcgaactccg agatgtcgat ctcggcgccg tcgtcgacga
     2281 gcgtggcatc ggtcatgaaa ccgaccagcg gaccatcgat cctgaatcgc tcccgccata
     2341 agggagcccc gaaccctgcc ttccaaccaa tggctcggtg accctgagag atgagcccag
     2401 accggagctc tagttggccc gccagaccac ttgccaatgc ggatcggctc attcggggag
     2461 ctcatatccg gctgcgtcga gcgagcccgc cccgctccag ccccagacca aggtcacatc
     2521 ctggtccccg gtgttgtcga agccgtgcca gagaccccgc cgggtaaaca ggacgtctcc
     2581 ttcgccggca cgctggtcct cgtcgtcggt gtagatcatt ccgctgccgg atagcacgat
     2641 caggaattcg tcggcattgg ggtgacgatg ccgatcgtgc cgggctcccg gcgggaggac
     2701 ggtccagccg agaagaaggt cgtcggagcc cgcctgggcc gcgtcgacca ggaactggac
     2761 tctcatgtcg acccacccct cgtcttcgcg cagccctcct tcgatcggga cgtcatggcg
     2821 gttgattcgg aatcggtcgg tcatccgtca ccccccaatc gtcggtagac atcgaagacg
     2881 gtgacgaagt cgcgatggct cagtccgaga ccatggcagg cgttgagcat ctcgttcgcc
     2941 gccgcggcga gtggcacggg actgccttga cgccgcccga ggtcgagggc gagcaacatg
     3001 tccttttgct gcagggtgac atctgccaat ggcttgtccg gcatgttgcc ctccaagatg
     3061 aaggggcccc gatagcccat cacgggtgag gcgacgacgc tcttgagcat cgcgtcgacc
     3121 gccacgacgc gatccacccc acctttctcg gccaacgcga cggcttcgct gaaagccacc
     3181 atctcgacga tgagacagag attgatcgcc accttcatct ggacggccat cccgctgggc
     3241 ccgatgtacg tgactgtggg cccgatcgcc tccagtaccg ggagcacctt ctcgaaggcg
     3301 gcgcggtcac cgccgaccat caccgaggcc ttgccctgat cgagtgaaac cgggctcccc
     3361 gataggggtg cgtcgagcat cgtccgtccc gcatcggcga aaagggcggc gattcgccgc
     3421 gatgcatcgg ggtcgatcgt gctcatgtcg gcatagacgc catcggaacc gagacccgac
     3481 aagacaccat cttccccgac ggccactgac tcgacggccg cggcatcggt cactatggag
     3541 aggactacgt cggccgtctc ggccacctgg cgaggggtgt cggcccatcg catgcccaga
     3601 tcgcggagag ggctggcctt ccctggggtt cggttccagc ccgtgacctc gtgcccggcg
     3661 gcgaggagtc ggggaacgat gccctggccc atgtcgccaa ggccgatgaa gccaacccgc
     3721 actcggtcct ccggagtcga tcgtcgagaa atcgagttta aggggcagcc tcccgaggaa
     3781 tacctgtccg ggatcactgg ttggcggagc gcatgcaatt cggagtcttc agcgtcggcg
     3841 atgtcaccac cgacccgaca acgggccgaa ccccgactga ggccgagcgg atcaaggcga
     3901 tggtcgccgt cgccctgaag accgaggaga tcggtctcga cgtgttcgcc accggcgagc
     3961 accacaatcc ccctttcgtc ccgtcctccc cgacgacgat gctcggctat ctggccgccc
     4021 gcaccgagcg gatcatcctc tccacggcta ccaccctcat caccaccaac gatcccgtga
     4081 agatcgctga ggacttcgcc atgctccagc atctggcgga tggacgcg
//
LOCUS       DASTXO010000103         2727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_42881, whole genome shotgun sequence.
ACCESSION   DASTXO010000103 DASTXO010000000
VERSION     DASTXO010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42881"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /gene="hisS"
                     /locus_tag="VFU96_02045"
     CDS             complement(<1..880)
                     /gene="hisS"
                     /locus_tag="VFU96_02045"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEU5112066.1"
                     /translation="MSFRPPKGTDDIGAPESNLWRDVLGEWERWADLFGYPLVLTPIF
                     EATELFERGVGSDTEVVTKQMYTFTDRGGRSLTLRPEGTAGVVRAFLDTGVRGPWKGS
                     YSGPMFRHERPQAGRRRQFWQVGVEYLDVEGAAADAEVVELGYRYLTAMQVPGLEVVL
                     NSLGDAVCRPGYVETLQVFLEGRRDLLCDDSIALIDVNPLRVLDCNVCAPVLTDAPSM
                     KDHLCEPCAEHYDQVRRSLEGLEVPFVEDARLVRGLDYYTRTAFEYLATDLEAAQNAV
                     GGGGRYDGLAESIGGRR"
     gene            complement(936..1151)
                     /locus_tag="VFU96_02050"
     CDS             complement(936..1151)
                     /locus_tag="VFU96_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112067.1"
                     /translation="MRPNAIDVALRRLAQTGRARIRVLTRDQHATETRLRNRAAWISK
                     PNARLRVARVTEGDGFLEVDLFLDGTD"
     gene            complement(1168..1806)
                     /locus_tag="VFU96_02055"
     CDS             complement(1168..1806)
                     /locus_tag="VFU96_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016811865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5112068.1"
                     /translation="MQIVQVPAWLTETNAWILAEQSGGRAIVVDAPPEPDAIGAILIQ
                     HDLTLAAVMLTHGHIDHTGGSGQLSRATGATVYVHPDDDFLTLHPVEQLRAMFGMTPP
                     GVYDVPERFEAIGDGDRLVLAGLDFEVRHTPGHTPGHCCFYLETEATLFSGDQLFAGS
                     IGRTDLPGGSLAALMASMDEKVMVLPDETRVLPGHGPETTIGRERIGNPFLQ"
     gene            complement(1806..>2727)
                     /locus_tag="VFU96_02060"
     CDS             complement(1806..>2727)
                     /locus_tag="VFU96_02060"
                     /inference="COORDINATES: protein motif:HMM:NF040246.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEU5112069.1"
                     /translation="HTEVGHRCVGARINGRLVPLSTRLQSGDIIEVLTSKSVEAGPSR
                     DWLAFVRTGRAKSKIRQWFTKERRDQALSEGKEALQALLKREAQGIPSAEKEALLESI
                     IAELGQKDVEALQVAIGEGTVSIDSVSTRLSRFLNPESEDEFFHPRRRRSLDTAEVVV
                     EGQDDMLIHLARCCSPVPGDPIVGYVTVGRGVSVHRADCTNVSSLAERRERTVEVSWP
                     PRSTSAFVVWIQVEALDRTRLLRDVTSVVSDTGGNITASSTHTGDDRVAILRYEVELS
                     DPSQLPKLLADLRGVDGVFSSFRLATDPAP"
BASE COUNT          476 a          892 c          880 g          479 t
ORIGIN      
        1 cgcgcctgcc cccgatcgat tcggccagcc cgtcatagcg tccaccgcct ccgacagcgt
       61 tctgggccgc ctcaaggtcg gtggcgagat attcgaacgc cgtgcgcgtg tagtaatcga
      121 gcccgcgaac cagccgcgca tcctcgacga acgggacttc gagaccctcg agtgacctcc
      181 tcacctggtc atagtgctcg gcgcacggct cgcagagatg atccttcatc gagggggcgt
      241 cggtcaggac cggcgcgcat acgttgcagt cgaggacccg caaaggattg acgtcgatca
      301 gcgcgatcga gtcgtcgcag agcagatcac gccgaccctc gaggaaaacc tggagcgtct
      361 cgacgtaccc gggccggcac accgcatcgc cgagcgaatt gagaacgact tccagcccgg
      421 ggacctgcat tgcggtcagg tatcggtagc cgagctcgac cacctcggca tccgcggccg
      481 caccttcgac gtcgagatac tccacaccga cctgccagaa ctgccggcgc cgccccgcct
      541 gggggcgctc atgccggaac atcggccccg agtacgagcc tttccacggg ccccgcacac
      601 cggtgtccag aaaagccctc acgactccgg ccgtcccctc gggacgaagc gtcagcgagc
      661 gacccccgcg atctgtgaag gtgtacatct gcttggtgac cacctcggtg tcgctcccga
      721 cccccctctc gaagagttcg gtggcctcga agatcggcgt caacaccagg gggtagccga
      781 acaggtcggc ccaacgctcc cactcgccca acacgtcacg ccagaggttg gattcgggcg
      841 cgccgatgtc atcggtgcct ttgggtggcc gaaagctcat gagcgctggc aggttaattg
      901 tggttcaaca cccaccgggc ctgggtggcg actggtcaat cggtcccgtc gagaaagagg
      961 tcgacttcga gaaagccatc gccttccgtg accctggcga cccgcagacg ggcattcggc
     1021 ttcgaaatcc acgcggcccg gttacggagt cgagtctcgg tggcgtgttg gtcgcgggtc
     1081 aggactcgga tacgagctct tccggtttga gccagtcgcc gcagggcgac gtcgatggcg
     1141 ttgggccgca tcggccgagc ttacgtgtca ttgcaggaac gggttcccga ttctttcccg
     1201 gccgatggtg gtctcgggac catgtcccgg caggaccctg gtctcgtccg gtaacaccat
     1261 caccttctcg tccatcgagg ccatgagagc ggccagcgac cctccgggca ggtcggttcg
     1321 accaatcgac ccggcgaaca gttgatcgcc tgagaacagg gtcgcctcgg tctcgaggta
     1381 gaaacagcaa tgccccgggg tatgtccggg tgtgtgccgc acctcgaagt cgagaccggc
     1441 aaggacgagt cgatctccgt cgccgattgc ctcgaagcgc tccggcacgt cgtagacacc
     1501 gggaggggtc atcccgaaca tcgcccgcaa ttgctcgacg ggatgcagtg tcaggaagtc
     1561 gtcatcggga tggacgtaga cggtcgcacc tgtagctcga gatagctgcc cgctgccccc
     1621 agtgtgatcg atgtggccgt gggtgagcat cacagctgcc agcgtcagat cgtgctggat
     1681 gaggatcgca ccgatcgcgt cgggttcggg aggggcgtcg accacgattg cccggccgcc
     1741 gctctgttcg gccaggatcc acgcgttggt ctctgtcagc caggccggca cctggacgat
     1801 ctgcatcagg gtgcaggatc ggtggcgagc cggaaggagg agaacacacc gtccactccg
     1861 cgcaggtcgg cgaggagctt gggcagttgg ctcggatcgg agagctctac ttcatagcgg
     1921 aggatggcta cccggtcgtc gccggtgtgt gtcgacgagg cggtgatgtt ccctccggtg
     1981 tcgctcacga cactggtcac atccctgagc agccgggtcc ggtccagggc ctcgacctgg
     2041 atccagacca cgaaggcgga cgtgcttcgt ggcggccagg acacctcgac ggtccgctcg
     2101 cgtcgctcgg cgagcgatga gacgttggtg caatccgctc gatgcaccga cacgccacgc
     2161 cccaccgtga cataaccgac gatggggtcg ccggggacgg gcgagcagca gcgggcaagg
     2221 tggatgagca tgtcgtcctg tccctctacc accacttcgg cggtgtccag ggagcgtctc
     2281 cgtcgtggat ggaagaactc atcctcgctc tcggggttga ggaacctgga gagccgggtc
     2341 gacaccgagt cgatcgagac cgtgccctcg ccgatcgcca cctggagcgc ttcgacgtcc
     2401 ttttgaccca gctcggcaat gatcgactcg aggagcgcct ccttctctgc ggaggggatc
     2461 ccctgcgcct cacgcttcag gagtgcctgc agcgcttctt tcccctcgga gagtgcctga
     2521 tcacgacgct ccttggtgaa ccattgcctg atcttggact tggctctgcc ggtgcggacg
     2581 aaggccagcc agtcacgact gggcccggcc tccaccgact tcgaggtcag cacttcgatt
     2641 atgtcccccg actggaggcg ggtcgagagt gggacgagcc ggccattgat cctggcaccg
     2701 acgcaccgat ggccgacttc agtgtgc
//
LOCUS       DASTXO010000104         3737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_264014, whole genome shotgun sequence.
ACCESSION   DASTXO010000104 DASTXO010000000
VERSION     DASTXO010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264014"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="VFU96_02065"
     CDS             complement(<1..796)
                     /locus_tag="VFU96_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU5112070.1"
                     /translation="MTQEAVLAERAAIDAEVEGKTIVDYLDRNAQRHGDQPAIHYKDG
                     DWKSLSWGEYRQAVHEAAAGFQDLGVGDGEFVAIMAGNRPEHVIADYAAIHSGATAVT
                     IYSTLTAPQIQYIADNCKATVAVLEDLEFMKRWEEIRSELPNLRYVVLMSGAENYDTA
                     DWVLSWDELLVRGKKRLADDPDAVSRTAGAITPDTLATLIYTSGTTGTPKGVMISHRN
                     VVWTAECLRRAANIDLGPRMVSYLPLAHIAERLATHYLATYLAGQVW"
     gene            complement(810..1952)
                     /locus_tag="VFU96_02070"
     CDS             complement(810..1952)
                     /locus_tag="VFU96_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017987134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEU5112071.1"
                     /translation="MTGPLQGMRVVEIASQGPAPFCGMILADLGADVVRVDRPDTVTS
                     GHTSSTRSDFMGRGKSSIGVDLKHEEGVRVVLRLVRGADALIEGLRPGVTERLGIGPA
                     PCLAQNPALVYGRMTGWGQDGPLSGRAGHDIDYIALSGVLAAIGPSERPLPPLNLVGD
                     FGGGGMLLAVGLLAAILHARETGRGQVIDAAMVDGSALLTTALHGHIAEGWWAARRED
                     NLLDGGAPFYAVYGTADDRHLAVGALEPQFFFALLEGLEIDPESLPAQGDRAGWPVIR
                     AALAAKFASESRDHWAEHFASMDACVAPVLDTTEAKEHSHNRARSVFIEVDGLTQPAP
                     APRFSDTPLPVPTGPAYPGKHTDQVLKAVGYSGTEVSMLRRVGAVA"
     gene            complement(1991..3463)
                     /locus_tag="VFU96_02075"
     CDS             complement(1991..3463)
                     /locus_tag="VFU96_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5112072.1"
                     /translation="MTAADFVFTYETIADPSLDIVSREGYHLITGYEVVDDKTVVFAF
                     DEIYAPWRTLFGSVLPAHVLEGEPFNTVWDDAITMGSGPFTFVEWVPEERITLARNEN
                     YWGDASGDVQTLNVLFLGGEEVQVDALADGEVDMIYPQPYVSLMDQIAGIGRVDSVTG
                     VGPVWEYFGFNQDDPRLRNLFVRQAIAQGINRDAIVEEVVRPITPEGQLLGNAVWPHN
                     SVHYEDHFNGPFPYDPAAAEALLTDNGCVRGEDGIYECDGERLSFSWATTSGSEARDV
                     AFELAQADLAAIGIEITPSFGPTSELLQTSNLQGGADVWQITNLAWVGSPDPSWGNSR
                     FYCQGNALNGFGELNFFRYCDEEVDALIRQTDVIVDPAERAATYNQADELWLGAVPMI
                     PMYQRPTLLAWDSIIQGPGDNPSEIGPFWNIADWSGKEEVNFGANLQPGSMNVMEPDG
                     NEIESIYVANAILQGAFTITPDFEYAPLLVESAEAIVNEG"
     gene            3438..>3737
                     /locus_tag="VFU96_02080"
     CDS             3438..>3737
                     /locus_tag="VFU96_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112073.1"
                     /translation="MKTKSAAVTGVPSAHAASGRMLYLTQNVSRHAPATVVVGAAVVV
                     VSGVVVFVIAGAAVVVGSASSVQATAAMTMKATTTDLIETALLTVSPRSRGHDRGS"
BASE COUNT          632 a         1227 c         1189 g          689 t
ORIGIN      
        1 accacacctg gccggccaga taggtggcca gatagtgggt ggcaagccgc tcggcgatgt
       61 gagccagtgg caggtaagac accattcgtg gcccgaggtc gatgttggcc gctcgacgca
      121 ggcattcggc ggtccacacg acgttgcgat ggctgatcat cacccccttc ggggtgccgg
      181 tggtgcctga ggtgtagatg agagtggcca gcgtgtcagg cgtgatggcg ccggcggtgc
      241 ggctgaccgc gtcggggtca tcggcgagac gcttcttgcc tcggaccaag agctcgtccc
      301 agctcaacac ccagtcggcc gtgtcgtagt tctcggcgcc ggacatcagg accacgtagc
      361 gcagattggg cagctcggac cggatctcct cccagcgctt catgaactcg agatcctcga
      421 gcactgcgac cgtcgccttg cagttgtcgg cgatgtattg aatctgggga gcggtcaggg
      481 tggagtagat ggtgactgcg gttgccccgg agtggatcgc cgcataatcg gcgatcacgt
      541 gctccgggcg gttcccggcc atgatggcga cgaattcgcc gtccccgacg cccaggtcct
      601 gaaacccggc cgccgcctcg tgcaccgcct ggcggtattc accccagctc agactcttcc
      661 agtcgccgtc cttgtagtgg atcgcgggct gatcaccgtg acgctgcgca tttcgatcga
      721 ggtagtcgac gatcgtcttg ccctctacct cggcgtcgat tgccgctcgc tccgccaaaa
      781 cggcttcctg ggtcattgct cctccgcgat caggccactg ctccgacccg gcggagcata
      841 cttacctcgg tcccgctata accgaccgct ttcagcacct gatctgtgtg cttgccgggg
      901 taggcaggcc ccgttggcac cggcaagggg gtgtccgaga acctgggggc cggcgccggc
      961 tgagtgagcc cgtcgacctc gatgaacacg ctccgcgccc ggttgtggga atgctctttc
     1021 gcctcggtgg tgtcgaggac aggggccacg caggcgtcca tcgacgcgaa atgctccgcc
     1081 caatggtcgc ggctctccga ggcgaacttc gccgccagcg cggccctgat caccggccag
     1141 cccgccctgt cgccctgtgc gggtagagac tccgggtcga tctcgagacc ttcgagaaga
     1201 gcgaagaaga actgcggctc gagcgcaccg acggcgagat ggcggtcgtc agccgtccca
     1261 tagactgcat agaagggagc gcctccgtcg aggagattgt cctcccgcct ggccgcccac
     1321 cacccctcgg cgatatggcc gtgcagggcc gtggtgagca gcgcggaccc atcgaccatg
     1381 gcggcgtcga tgacctggcc cctgccggtc tcacgtgcgt ggagaatggc ggcgagaagg
     1441 ccgactgcga ggagcatgcc ccctcctccg aaatcaccca ccaggttcaa aggagggagt
     1501 ggccgctcgc tcgggccgat cgcggccagg acgccggaca gagcgatgta gtcgatgtcg
     1561 tggccggcac gccctgacag cgggccatcc tggccccatc cggtcatgcg tccgtaaacc
     1621 agcgcggggt tttgggcgag acacggagca gggccgatgc cgaggcgctc ggtcaccccg
     1681 ggcctgagac cctcgatcag agcgtcggcc ccccgcacca gcctcagcac gactctcacc
     1741 ccctcttcgt gcttgaggtc gactccgatc gagctcttgc cgcgacccat gaagtcgctt
     1801 ctcgtcgagc tggtgtgccc gctggtgacg gtgtctgggc ggtccaccct gaccacgtcc
     1861 gcaccgaggt cggcaaggat cattccacag aacggcgccg gcccctggga ggcgatctcc
     1921 acaaccctca ttccctgcaa ggggcctgtc atctccggcg caacctagct gcgaccggtc
     1981 caatcaaagc tcagccttcg ttgactatcg cctccgcgct ctcgaccaga aggggggcgt
     2041 actcgaagtc cggggtgatg gtgaaggcac cctggaggat ggcgttggcg acatagatgc
     2101 tctctatctc gttgccgtcg ggctccatga cgttcatcga ccccggttgc aggttcgccc
     2161 cgaagttgac ttcctccttg ccgctccaat cggcgatgtt ccaaaagggc ccaatctcgg
     2221 acgggttgtc accgggcccc tggatgatcg aatcccaggc gaggagcgtc ggtctctgat
     2281 acatcgggat catggggacc gcgcccagcc acagctcgtc ggcctggttg taggtagcgg
     2341 cgcgctccgc ggggtcgacg atcacgtcgg tctgccggat gagtgcatcc acctcctcat
     2401 cgcagtatct gaagaaattc agctcaccaa agccattcaa ggcgttgccc tggcagtaga
     2461 agcgcgagtt gccccaggac gggtcgggtg agccgaccca agcgaggttc gtgatctgcc
     2521 agacgtctgc cccaccctga aggttgctcg tctgcagcaa ctcggatgtc ggcccgaagc
     2581 tcggtgttat ctcgatcccg atcgcggcga gatcggcctg ggccagctcg aaggccacat
     2641 cgcgtgcctc gctgccggat gtggtcgccc aggagaagga gagtcgttcg ccgtcgcatt
     2701 catatatccc gtcctcgccc ctcacgcagc cgttatccgt cagcagggct tctgccgcag
     2761 ccgggtcgta tgggaacggg ccgttgaaat ggtcctcgta gtgcacgctg ttgtggggcc
     2821 atactgcgtt cccgagaagc tggccttcgg gagtgatcgg tcggacgacc tcctccacga
     2881 tggcgtcccg gttgattccc tgggcaatcg cctgtcgcac gaaaaggttc cgcagccgcg
     2941 ggtcatcctg gttgaaaccg aagtactccc acacggggcc aacacccgtc accgaatcga
     3001 ctcggccgat cccggctatc tgatccatga gggacacata gggctggggg tagatcatgt
     3061 cgacctcacc gtctgccagg gcatccacct ggacctcctc gccgcccagg aacaggacat
     3121 tcagggtttg cacgtcaccc gaggcatcgc cccagtagtt ctcgtttcga gccagcgtga
     3181 tccgctcctc gggcacccat tcgacgaagg tgaacggccc cgagcccatc gtgatcgcat
     3241 catcccacac cgtgttgaac ggctcgcctt ccaggacatg agccgggagg accgagccga
     3301 acaaggtccg ccagggcgcg tatatctcat cgaaggcgaa caccaccgtc ttgtcatcca
     3361 cgacctcgta gccggtgatc aggtggtatc cctcgcgaga gacgatgtcg agcgatggat
     3421 cggcgatcgt ctcgtaggtg aagacgaaat cggccgcggt caccggtgtg ccatcggccc
     3481 acgccgcctc cggccggatg ttgtatctga cgcagaatgt gtcgcgacat gcgcccgcga
     3541 cggtcgtcgt gggggccgcg gtcgtcgttg tctctggcgt cgtcgtgttc gtgattgccg
     3601 gcgcggccgt agtcgttggc tcagcctcgt cggtgcaggc gaccgccgcc atgaccatga
     3661 aggcgacaac gactgatctg atcgaaacgg ccctgctcac ggtgtcaccc cggagtcgtg
     3721 gtcatgatcg aggatcg
//
LOCUS       DASTXO010000105        12464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_411578, whole genome shotgun sequence.
ACCESSION   DASTXO010000105 DASTXO010000000
VERSION     DASTXO010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 12464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12464
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411578"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VFU96_02085"
     CDS             complement(<1..245)
                     /locus_tag="VFU96_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl transferase family protein"
                     /protein_id="HEU5112074.1"
                     /translation="MTRVLAILGPTAAGKTETAIRLAEHFGGEVISVDSMQVYRGMDI
                     GTAKPTLLERRGIPHHLVDVAAPGEEFSVARFRTLGRG"
     gene            complement(242..1630)
                     /gene="miaB"
                     /locus_tag="VFU96_02090"
     CDS             complement(242..1630)
                     /gene="miaB"
                     /locus_tag="VFU96_02090"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEU5112075.1"
                     /translation="MTQSVLLPNPTLREPARRVPTRVGRRYFIRTFGCQMNEHDSERI
                     AGLFEMDGMTAAGGFESADVVYINTCTIRENADNRMYGNLGQLKAVKDLNPEMLLVVG
                     GCAAQKERDLVRERAPWVDVVMGTHNLDRVLDLMDHAENWGPITEVVDELQAMPSSLP
                     VKRELEHSAWVTIQIGCNNTCTFCIVPSVRGVEVSRRPGDIVREVERLAADGVVEVTL
                     LGQNVDTYGRDLALNGKRRPIFAELLRRVGEVDGIRRVRFTSPHPNDFREDVARAMAE
                     TYEVCEHLHFPLQSGSDRILRAMHRGYNRKKYTERLAMAREIIPGLAVSTDVIVGFPG
                     ESEEDFESTLELVAEARFDSAFTFIFSPRPGTAAAMLTEQFVPDELIRKRFTRLSELQ
                     DRISGDKNAELVGSTVEVLSEGPSRKNPQVATTRSRTGKVVHVAGAHRAGSFLMAEIG
                     MAHAHHLVGSPV"
     gene            complement(1641..3182)
                     /gene="rny"
                     /locus_tag="VFU96_02095"
     CDS             complement(1641..3182)
                     /gene="rny"
                     /locus_tag="VFU96_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEU5112076.1"
                     /translation="MDTIPVIGLTILGVVLGLGFGYLIARLAIARRRERDGVSAEDVV
                     TSARLDAQRILAKAEEEGRAKAETYREREDANLEHRRLEMSNLESRLGQREETLDQRA
                     SNLAQREKMIIDREEELNVSRGELERLKEEARFRLEQVAGIDVTAAKTEMLAQVEDEA
                     RREAMIVVRDLEIKAREEADRRARRILATAIQRLSSEVVTETTVSVVQLPSDDMKGRI
                     IGREGRNIRHLESVTGTSLIVDDTPEAVSVSSFDPVRREVARITLEKLVADGRIHPAS
                     IEEAYEKAQAEVEQSIRDAGEWALVEAGVSRMHSEIVTLMGRLKYRTSYGQNVLNHLV
                     ETAHIASMLSSELGIDPAPVKRAALLHDLGKAVTHEVEGSHALIGAEIARRFDEDPAV
                     VHGIEAHHNEVEPRTVLAVIVQAADAVSAARPGARRETLEGYVKRLERLETLAMEFPG
                     VDHVFALQAGREVRVVVDPGNVSDVEARELSRRIARKLEEDLQYPGQIQVTVIREFRA
                     TDYAR"
     gene            complement(3398..4456)
                     /gene="recA"
                     /locus_tag="VFU96_02100"
     CDS             complement(3398..4456)
                     /gene="recA"
                     /locus_tag="VFU96_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015787075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEU5112077.1"
                     /translation="MERDKALDMAVSQIERQFGKGAIMKMGEAANRKVDVISTGALSL
                     DLALGIGGVPRGRIVELYGPESSGKTSLALHIVAEAQRNGGIAAFIDAEHALDPIYAK
                     AIGVDVDELLISQPDTGEQALEIADMLIRSGALDVLVIDSVAALVPRAEIEGEMGDAH
                     VGLQARLMSQALRKLAGTLNKSRTTAVFINQLREKIGVMYGSPETTPGGRALKFYSSV
                     RLDIRRIETIKDGTESVGNRVKVKVAKNKMAPPFRLAEFDIMFGEGISREGSLLDVAV
                     EHGVVRKSGAWFTFDDDQLGQGRENAKRFLRENPEVAMQLQAKVYELVGLGESTSEET
                     TEEEVVAGGEDSNRPEEQ"
     gene            complement(4640..5191)
                     /gene="thpR"
                     /locus_tag="VFU96_02105"
     CDS             complement(4640..5191)
                     /gene="thpR"
                     /locus_tag="VFU96_02105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEU5112078.1"
                     /translation="MAGVAARIFVAVPLPDEQRTALADSLRPFDIPGRVVTPQNWHIT
                     LRFLGWTDDVAFDRMLAALDETDLGAPFEVTLGEMGAFPRPRNATVIWLAVSRGSNRL
                     EELAAAAEEAAQRAGFAREDRPFRAHLTLSRVRPSENVGPVVEAYPGADLGWRCESVV
                     VYRSHLGRGGARYEPLETFPLAR"
     gene            complement(5179..6414)
                     /locus_tag="VFU96_02110"
     CDS             complement(5179..6414)
                     /locus_tag="VFU96_02110"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEU5112079.1"
                     /translation="MIVEVIAVGTELLLGQIVNSNTSTMGAALAERGIDAHYQQTVGD
                     NLTRIADAIRVALDRSDAVVTTGGIGPTQDDITREALCQVTGRDMVFSEDYADRLREW
                     WSRRGREMPQSNLRQAYYPEGAEMLPNPRGTAPGLVLEHEGKLIFCVPGVPAEMEHLM
                     FEEVLPRLAAASGEEQAIASRIIRTWGRPESEVAEVLDDLYTSSVNPSLAFLASNSEI
                     KIRVTAKAETAVAARHLIEPMEMEVRSRLGDNVFGSDDETIERVLMQLLLERGYTIGT
                     AESMTGGMVSARLTELPGSSAVVKGGLVAYDSELKHRLLGLTDITEVVTIDAAVAMAK
                     GARALLDADVAIAVTGSAGPEPMEKPVGTVIVGVATPDDVRARELRFSGDRERLRTYG
                     TTAALHLARLALIGRWWQE"
     gene            complement(6433..6687)
                     /locus_tag="VFU96_02115"
     CDS             complement(6433..6687)
                     /locus_tag="VFU96_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112080.1"
                     /translation="MQPKAEAFVEEAIRAVGIHDVAEARIAVIQAYDLDHALGPLVDA
                     VHLACAEIEEDGLVSIATWNTLADAVGPGHLLVVVESSRE"
     gene            complement(6687..7742)
                     /locus_tag="VFU96_02120"
     CDS             complement(6687..7742)
                     /locus_tag="VFU96_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112081.1"
                     /translation="MNIVHVVGTGTIGEPLIGLLADNKEHFGIEEVTFHKRTPLLTEK
                     AKVADLEGRGAVLAVDEERFSSFQELGHAPKYEAREAIERATVVIDCTPVGNGMKEEF
                     YSHADGPRGFMAQGSEFGFGKMYARGINDSALVAGEDRFLQIVSCNTHNIAALVRSLA
                     IDADGTNHLEDGRFLCMRRANDISQDDGFVASPTVESHKDERFGTHHARDAHELFDTM
                     GYDLNLYSSAVKINTQYMHSIQFSLQLDEEITVDEAKERLMANTRVAVTHKKSANQVF
                     SFGRDHGYFGRIMSQTVVALSTLAVRNGNEVVGFSFTPQDGNPLLSTVAAMLWYLDPE
                     TLDDRIDILRRYLFREI"
     gene            complement(7818..9095)
                     /gene="aceA"
                     /locus_tag="VFU96_02125"
     CDS             complement(7818..9095)
                     /gene="aceA"
                     /locus_tag="VFU96_02125"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011230179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEU5112082.1"
                     /translation="MSHFANEASRLADEWRANPRWEGVTRDYAAADVVRLRGSIREQS
                     TLANRMAIALWERLTTEDYISALGALSGGQAVQMVKAGLKSIYLSGWQVAADGNLAGD
                     VYPDQSLYPSNSAPALVERINNALRRADQIHWAEGKTDLDWFVPIIADGEAGFGGALN
                     VFELTKRFIQAGAAGVHFEDQLASEKKCGHMGGKVLVPTAQHERTLQAARLAADVMDV
                     PTVIVARTDANGATLLTSDIDERDRRFVTGERTPEGFHVVTGGLESAIARGLSYSPYA
                     DLIWCETAQPDLDEARTFAEAIHAEYPGKMLAYNCSPSFNWSKYLDEATIARFQKELG
                     TMGYRYQFITLAGFHALNASMFELARGYAEQGMPAYVELQNLEFSMEGDGYTATRHQR
                     EVGAGYFDLVSRVIAGDGSATLAMEGSTEQAQF"
     gene            complement(9108..10667)
                     /gene="aceB"
                     /locus_tag="VFU96_02130"
     CDS             complement(9108..10667)
                     /gene="aceB"
                     /locus_tag="VFU96_02130"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEU5112083.1"
                     /translation="MNQFRDTEIERRVLTPEAVAFLTDLEQEFGWQRGQLLADRHTRQ
                     RRLDDGEKPDFLPETREIRESDWGIDVVPADLSDRRVEITGPVDAKMMINALNSGARV
                     FMADFEDATSPTWANLLEGQANLVDAYRRELRLDTGEKTYELADETATLLVRPRGWHL
                     GEKHFLVDGRPMSGSLFDFGLYLFHGGRAAIEAGTGPYLYLPKLESHREARLWNDVFV
                     WTQDRLGIPRGTIKATVLIETILAAFEMDEILYELREHAAGLNAGRWDYIFSVIKKFR
                     NDPSFVLPDRSSVTMTVPFMRAYTELMVKTCHERGAHAIGGMAAFIPNRRDASVTEKA
                     IAAVTADKAREAGSGCDGTWVAHPDLVPVAMKEFDAVLGDRPNQLDRRRDDVDVDAGD
                     LLDFDFPGSVTNAGLETNVSVGIRYLSSWLSGTGAAAIDNLMEDVATAEISRSQVWQW
                     VRHGVATEEGAPVTESRIRSMAGALVDRLEGDGNPYGDRLPVARKVFEDVALGEGFVE
                     FLTLPAYELIN"
     gene            10752..12200
                     /locus_tag="VFU96_02135"
     CDS             10752..12200
                     /locus_tag="VFU96_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5112084.1"
                     /translation="MTQSQQLDPLILGRRLRYHRRERGMTLDELGAVVHRPAPYLSLL
                     ENGKKQPRLNLVIELAAALDVDIAELLDPSPPNRRDALEIALLRSQESPFFDSLHLPS
                     VKPSAKLDNETLTHLVGLHDALRERSNLGSAGSEEVRRANGEVTSWLAGNDGYLEEVE
                     TEARNALASSGYSGEGPFSSRNLLDLTASAGFELHPVEDMPTFARSIIDLDHHRVYIA
                     QRDALRTRQARKAVLQTLAGFLLGHETDPDLDTFLRQRVETAYFAAAVLAPEDPVVSR
                     LGGAKDNHDIDVEDVKELFYISYEMAAWRTVNLATRHLDITTHLIVSDEMGTVVKAYS
                     NDGVPIPRDANGGMETQRLCRRWGARITFDSAERYGTHQQYTDTPEGTFFCTTHVEAG
                     RDPAHAVTVGVPFSEARWFRGRETTNRQTSTCPDPSCCPMAPPALQQKWSDKVIVSAR
                     SQNRILGLLAPDPYPELDMPEVLDLVEAHAES"
     gene            complement(12201..>12464)
                     /locus_tag="VFU96_02140"
     CDS             complement(12201..>12464)
                     /locus_tag="VFU96_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HEU5112085.1"
                     /translation="YVDTSEQLRDVMSRSQRMQVFIASGYYDLATPHYATEYTVSHLG
                     LDESLRKNIQMEYYEAGHMMYLQRESLVALAGHLRAFIEDTAR"
BASE COUNT         2168 a         4111 c         3830 g         2355 t
ORIGIN      
        1 ccccgaccca gcgtgcgaaa acgggccacg gagaactcct cgcccggggc ggcgacgtcg
       61 acgagatgat gcggtatccc ccgtcgctcc agcagcgtgg gctttgctgt cccgatatcc
      121 atgccccggt agacctgcat cgaatcgacc gagatgactt ctcctccgaa atgctccgcc
      181 aggcggatcg ccgtctcggt cttgcctgcc gcagttggcc cgagaatggc caggacccgg
      241 gtcagacagg gctccctacg agatgatgcg catgggccat cccgatctcg gccatgagga
      301 aggaccctgc gcgatgtgcg cccgccacat gcactacctt gccggtcctg ctgcgggtgg
      361 ttgccacctg cggattcttg cgagacgggc cctcgctgag cacctcgacc gtgctgccga
      421 cgagctcggc gttcttatcc cctgagatcc ggtcctgcaa ttcgctgaga cgagtgaatc
      481 tcttccggat cagctcatcc ggcacgaatt gttcggtgag catcgcggca gccgtgcctg
      541 gtcgcggtga gaaaatgaag gtgaaggcac tgtcgaatcg ggcctccgcc acgagctcga
      601 gggtggactc gaagtcctcc tcgctttccc ccgggaatcc gacgatgacg tcggtggaca
      661 cggcgagacc ggggatgatc tcccgggcca tcgccaaccg ctcggtgtac ttcttgcggt
      721 tgtagccacg atgcatcgcc cgcaggatgc ggtcggagcc ggactgcaat ggaaaatgga
      781 ggtgctcaca gacttcatag gtttcggcca tagcccgtgc aacgtcctcg cgaaagtcat
      841 tcggatgggg gctcgtgaat cgcactctgc ggatgccgtc gacttcgccc actcgccgca
      901 agagctcggc gaagatgggc cgccgcttcc cgttcaaagc gaggtcccgg ccataggtgt
      961 cgacgttctg gccgagcagt gtcacctcga ccacgccgtc ggcggcgaga cgctccacct
     1021 ccctcacgat gtcgccgggc cgacgggaca cttcgacgcc gcgcaccgag ggcacgatgc
     1081 agaaggtgca tgtgttgttg catccgatct ggatcgtcac ccaggcggag tgctccaact
     1141 ccctcttgac ggggagcgac gagggcatgg cctggagctc gtcgacgacc tcggttatgg
     1201 ggccccagtt ctcggcatgg tccatgaggt cgagcacgcg gtcgaggttg tgggtgccca
     1261 tgaccacatc gacccaggga gcccgctccc ggaccagatc gcgctccttc tgtgcagcgc
     1321 acccacccac cacaagcagc atttcggggt tgagatcctt caccgccttc agctgtccca
     1381 gattgccgta catccggttg tcggcgtttt ccctgatggt gcaggtgttg atgtagacca
     1441 catcggcgga ttcgaagccg ccggcggcgg tcatgccatc catctcgaac agcccggcga
     1501 tccgctccga gtcgtgctcg ttcatctgac agccaaaggt gcgtatgaaa tagcgccgac
     1561 caacccgggt cgggacacga cgggccggtt cgcgcagcgt cggattgggg agaaggacag
     1621 actgggtcat gtggtcgatt tcagcgggcg tagtccgtcg cccgaaactc ccggatcacg
     1681 gtcacttgta tctgacccgg gtattggagg tcttcctcga gtttacgggc gatccggcgc
     1741 gaaagctccc gggcctcgac atcggagacg ttgcccgggt cgactacgac cctgacttcg
     1801 cggccggcct ggagagcgaa gacgtggtcg acgccaggga actccatggc cagggtctcc
     1861 agacgttcga gacgcttcac gtagccttcg agtgtctccc tgcgcgcccc gggccgggcc
     1921 gcggacaccg cgtcggcggc ctggacgatc accgccagga ctgtcctcgg ctccacctcg
     1981 ttgtgatgtg cctcgatgcc gtgcaccaca gccgggtctt catcgaagcg acgggcgatc
     2041 tcggccccga tcagggcgtg ggacccctcg acctcgtggg tgaccgcctt gccgaggtca
     2101 tggagcagcg ccgcccgctt caccggcgcc ggatcgatcc ccaactccga ggagagcatg
     2161 gaggcgatgt gcgccgtctc caccagatgg ttgagcacgt tctggccata tgaggtccgg
     2221 tacttgagac ggcccatgag ggtgacgatc tccgagtgca tgcgggagac cccggcctcg
     2281 acgagagccc actcgccggc gtcacggatg ctctgctcca cttcggcttg tgccttctcg
     2341 taggcctcct cgatggacgc aggatggatc cgaccgtccg ctaccagctt ctccagggtg
     2401 atcctggcca cttcccgccg cacggggtcg aacgaggaga cggagaccgc ctccggcgtg
     2461 tcgtcgacga tcaggctggt cccggtcacc gactcgagat gtcgaatgtt gcgtccttca
     2521 cgaccgatga tccggccctt catgtcgtcg gatgggagct gtaccaccga gactgtggtc
     2581 tcggtgacca cctcggagga gagacgctgg attgccgtgg ccaggatccg ccttgcacgc
     2641 cgatcggctt cctcccgggc cttgatctcc agatctctga ccacgatcat cgcctcgcgg
     2701 cgggcctcgt cttcgacctg ggccagcatc tcggtcttgg cggcagtcac gtcgatgccg
     2761 gcaacctgct ccagtcggaa gcgtgcctcc tctttgagac gctccagctc ccctcgagat
     2821 acgttgagct cctcttcccg gtcgatgatc atcttttccc gttgggcgag gttggaggcg
     2881 cgctgatcga gcgtctcctc acgctgtccc agcctggatt cgagattcga catctcgagg
     2941 cggcgatgct cgagattggc atcctcccgc tcccgatagg tctccgcctt ggcccggccc
     3001 tcctcctccg ccttggccag aatgcgttgc gcgtcgagac gggcgctggt gacgacgtcc
     3061 tcggcggaga ccccgtcgcg ctctcttctt cgggcgatgg cgagacgggc gatcagatag
     3121 ccgaagccca gaccgagaac gaccccgagg atcgtcaacc cgatgacggg gatggtgtcc
     3181 atggtcggcc cctttcccgg tcggagaatg aacgtgccga gcccctaccc gatatcaggt
     3241 cggtggcccg gcacacgaag agaagctgac taacgaggcg ggcaggtcac ccgtcggttc
     3301 gttggctcct tctgttcacg tcttctttcc ggtttgcagt tcaaaggtgt gttcgtgtga
     3361 ggtctcgatg ctacacgcac atcgcgaaat cggacaccta ctgctcctcg ggcctgttgg
     3421 agtcctcccc gccggccacg acctcttcct cggtggtctc ctccgaggtc gactcaccga
     3481 gtccgaccaa ctcgtagacc ttggcctgga gttgcatcgc cacctcgggg ttctcccgca
     3541 gaaaccgctt cgcattctcc cggccctggc ccagctgatc gtcgtcgaag gtgaaccagg
     3601 ccccgctctt gcggaccaca ccatgctcga cggcgacatc gaggagactg ccttctcggg
     3661 agatcccctc gccgaacatg atgtcgaatt cggccagacg gaagggtggg gccatcttgt
     3721 tcttggccac cttgaccttg acccggttgc cgacgctctc ggtgccatcc ttgatggtct
     3781 cgatacgccg gatgtcgaga cgcaccgacg agtagaactt gagcgcccga ccaccggggg
     3841 tggtctcggg tgagccatac atcaccccta tcttctcccg aagctggttg atgaagacgg
     3901 cggtggtcct cgatttgttg agcgtcccgg ccagcttgcg cagagcctgg gacatgaggc
     3961 gcgcctggag cccgacatgg gcatcaccca tctcgccttc gatctcggcc cgtggcacca
     4021 gggcagccac cgagtcgatg acgaggacgt cgagtgctcc ggaacgaatc agcatgtcgg
     4081 cgatctcgag cgcctgctcc ccagtgtcgg gctgggagat gagcaactcg tcgacatcga
     4141 ctccaatcgc cttggcatag atcgggtcga gggcgtgctc ggcgtcgatg aaggcggcga
     4201 tcccgccatt gcgctgggcc tcggcgacga tatggagggc gagactggtc ttacccgagc
     4261 tctccggccc gtagagctcg acgatccgcc cacgggggac accgccgatg cccagggcga
     4321 gatcgaggga gagggcgccg gtggagatca cgtcaacctt gcggttggcc gcctcaccca
     4381 tcttcatgat cgcccctttg ccgaactgac gctcgatctg gctgaccgcc atatccagtg
     4441 ctttgtcgcg ttccatctgt gactccttct gcggctcctt ctgtggcccc ttctcgggct
     4501 ctttcttcga tcccatgtgc ccggtttcct ctgacggtcc gtatctcggc caaggtatct
     4561 gccaggtgag acagatctcg tctcattccc gtttctcgac gatctggata ttagaccgaa
     4621 caggtgttcg tcatcggatt taccgggcga gcgggaaggt ttcaagtggt tcgtacctgg
     4681 ccccgcctcg cccgagatgt gaccgataga ccaccaccga ctcgcaccgc caaccgaggt
     4741 ctgcaccggg gtaggcctcc accaccgggc ccacgttctc cgatggcctc acccggctga
     4801 gagtgagatg agcccgaaac ggccggtcct ctcgtgcgaa gccggctctc tgcgccgcct
     4861 cctcggctgc cgccgccaat tcctcgagtc ggttcgaccc acgggacacg gccaaccaga
     4921 tcacggtggc attcctcggc cggggaaacg cgcccatctc ccccagcgtc acctcgaatg
     4981 gagcgccgag gtcggtctcg tctagggcag cgagcatccg gtcgaaggcc acgtcgtcgg
     5041 tccacccgag aaagcgaagg gtgatgtgcc agttctgcgg tgtcaccacc ctgcccggga
     5101 tgtcgaacgg ccggagactg tcggccagtg ccgtgcgctg ctcatcgggg agaggcaccg
     5161 ccacgaagat gcgggcggct actcctgcca ccatctgccg atgagggcca gccgggcgag
     5221 atgcaaggcc gccgtcgtgc catacgttcg caaccgctcc cgatcgccac tgaatctcag
     5281 ctcccgcgcc cgcacgtcgt caggagtcgc gactccgaca atcacggtcc cgaccggctt
     5341 ctccatcggc tcgggacccg ccgatcccgt caccgcgatc gcgacatcgg catcgagcag
     5401 ggctctcgct ccttttgcca tggcaacggc ggcgtcgatg gtcacgactt ctgtgatgtc
     5461 ggtcagtccg agcaagcgat gtttcaactc ggagtcgtac gcaaccagcc cacccttgac
     5521 caccgccgac gagccaggca gctccgtcag tcgggcgctg accatgcccc cggtcatcga
     5581 ttctgcagtt ccgatcgtgt agccacgctc caagagaagc tgcatgagga ctcgctcgat
     5641 cgtctcgtcg tcggacccga acacgttgtc cccgagacgc gagcgcacct ccatctccat
     5701 cggctcgatg agatggcggg ccgcgaccgc ggtctcagcc ttggcggtga cccggatctt
     5761 gatctccgaa ttcgatgcga gaaaggccaa tgaagggttc accgacgacg tgtagagatc
     5821 atcgaggacc tcggccacct cggactcggg gcgaccccag gtgcggatga tgcggctggc
     5881 gatcgcctgc tcctcaccgg aggccgcggc cagcctcggc aagacctctt cgaacatcag
     5941 atgctccatc tcggcgggga cgccggggac acagaagatc agcttgccct cgtgctcgag
     6001 gaccaagccc ggcgccgtgc cccgcggatt ggggagcatc tccgcccctt ctgggtaata
     6061 cgcctgacgg agattgctct ggggcatctc ccggcctcgt cgcgaccacc attcccgtaa
     6121 ccgatcggcg tagtcctcgc tgaacaccat gtcccgcccg gtgacctgac acaacgcctc
     6181 cctggtgatg tcgtcctgcg tgggcccgat gcccccggtc gtcaccaccg catcagagcg
     6241 gtcgagcgcg accctgatcg catcggcgat acgggtcaga ttgtcaccga ccgtttgctg
     6301 gtagtgggcg tcgatgccac gctcggcgag ggcggccccc atggtcgacg tgttcgagtt
     6361 gacgatctgg ccgaggagga gctcggtccc caccgcgatg acctcgacga tcacgattgg
     6421 tcgatcaacc ggctactcgc gcgacgactc gaccacgacc aggagatggc cggggccgac
     6481 cgcgtcggcg agtgtgttcc aggtggcgat gctcaccaag ccatcctcct cgatctctgc
     6541 gcaagccaga tgaaccgcat ccaccagggg gccaagagcg tggtcgaggt cgtaggcctg
     6601 gatgacagcg atgcgggctt ctgcgacatc gtggatgcca accgccctga tcgcctcctc
     6661 gacgaaggcc tccgccttcg gttgcatcag atctcgcgga acaggtagcg gcggaggatg
     6721 tcgattcggt cgtccagcgt ctccgggtcg agataccaga gcatcgcggc aaccgtggag
     6781 agcagcggat tcccatcctg gggggtgaag gagaacccga cgacctcgtt gccgttgcgg
     6841 actgccagcg tgctcagcgc caccaccgtc tgactcatga tcctgccgaa gtagccgtgg
     6901 tcgcgaccga aggagaacac ctggttggcc gatttcttgt gggtgaccgc aactctggtg
     6961 ttggccatca gacgctcctt ggcctcgtcc accgtgatct cctcgtccag ttgcagcgag
     7021 aactggatcg agtgcatgta ctgagtgttg atcttgaccg ccgacgagta gagattgagg
     7081 tcgtatccca tggtgtcgaa caactcatgg gcgtctcgag catgatgcgt cccgaagcgc
     7141 tcgtccttgt gtgactccac cgtcgggctg gcgacgaagc cgtcgtcctg gcttatgtcg
     7201 ttggcccgtc gcatacagag gaaacggccg tcctcgaggt ggttggtccc gtcggcgtct
     7261 atggccagtg agcggacgag ggctgcgatg ttgtgcgtgt tgcaggagac gatctggagg
     7321 aatctgtcct cgccggcgac cagggcgctg tcattgatcc cccgggcgta catcttcccg
     7381 aacccgaact ccgagccctg agccatgaag cctctcggtc cgtcggcatg gctgtagaac
     7441 tcctctttca tgccgttgcc caccggcgtg cagtcgatga cgacagtcgc ccgttcgatc
     7501 gcctctcggg cctcgtactt gggagcatgg cccagttcct ggaatgacga gaaccgctct
     7561 tcgtcgacgg cgagcaccgc gcctcttccc tcgagatctg cgacctttgc cttctcggtg
     7621 agcagagggg tgcgcttgtg aaacgtcacc tcctcgatgc cgaaatgctc cttgttgtcg
     7681 gcgaggaggc cgatgagcgg ctcaccgatg gtgccggtgc ccaccacgtg gacgatgttc
     7741 atgacgggtt ccctccgaga acagcgctgc tcctggggct cacactactt gccccccctt
     7801 ttcctttccc aaactcctca gaattgcgcc tgctctgtcg agccctccat ggccagggtc
     7861 gctgagccgt cgccggcgat gacgcggctc accaggtcga agtacccagc gccgacttcg
     7921 cgctgatgtc tggtggcggt gtacccgtca ccctccatcg agaactccag attctgcagc
     7981 tccacgtagg ccggcatccc ttgctcggca tagccccggg ccagctcgaa catggaggcg
     8041 ttgagtgcat ggaatccggc gagggtgatg aactggtatc ggtagcccat cgtgcccagc
     8101 tccttctgga aacgggcgat cgtcgcctca tcgagatact tggaccagtt gaacgaaggc
     8161 gagcagttgt aggccaacat cttgcccgga tactcggcgt gaatcgcctc ggcgaaggtc
     8221 cgggcctcgt ccaggtcggg ttgggccgtc tcgcaccaga tcaagtcggc gtacggcgag
     8281 taggaaaggc cgcgggcaat cgcagattcg aggccaccgg tgaccacgtg aaatccctcc
     8341 ggcgtgcgct cacctgtgac aaagcggcgg tcccgctcgt cgatatcgga ggtgagcagg
     8401 gtcgcgccgt tggcgtcggt cctggccacg atcacagtgg gcacgtccat cacgtcggcg
     8461 gccaaacggg ccgcctgcag ggttcgctcg tgctgcgcgg tcggcaccag gaccttgccc
     8521 cccatgtgac cgcatttctt ctcggacgcc agctggtcct cgaagtggac cccggctgca
     8581 ccggcctgga tgaacctctt ggtgagctcg aagacgttga gggcaccccc gaagccggcc
     8641 tccccgtcgg cgatgatggg gacgaaccaa tcgagatcgg tcttcccttc ggcccaatgg
     8701 atctggtcgg cgcggcgcaa tgcgttgttg atcctctcga cgagcgcggg agccgaattg
     8761 gacggataca gcgattgatc gggatagacg tcgccggcga gattgccatc cgccgcgacc
     8821 tgccatccgg agaggtagat cgacttgaga cccgccttca ccatctgcac cgcctggcca
     8881 ccactgagcg cgcccaatgc gctgatgtag tcctcggtgg tgagacgctc ccatagagcg
     8941 atggccatgc gattggccaa tgtcgactgc tctctgatgc tgccccggag ccggacgacg
     9001 tccgctgcgg cgtaatcccg ggtcacgcct tcccaacgag ggttggccct ccactcgtcg
     9061 gccagccggc tggcctcatt ggcgaagtgc gacatgtatc tctcccctca gttgatcaac
     9121 tcgtacgcgg gcaatgtcag gaactcgacg aagccctcgc cgagggcaac gtcttcgaat
     9181 accttccggg cgaccggcag gcgatcgcca tatgggttgc cgtcaccctc gaggcgatcg
     9241 accagcgctc cagccatcga ccggatccgg gactcggtaa ccggggcgcc ctcctcggtc
     9301 gcgactccgt gtcgcaccca ctgccacacc tgcgatctcg agatctcggc cgtcgcgacg
     9361 tcctccatga gattgtcgat cgcggcggcc cccgttccgg acaaccagga ggagaggtag
     9421 cggatgccaa ccgagacatt cgtctccagc ccggcgttgg tcaccgagcc ggggaaatcg
     9481 aagtcgagca agtcgcccgc gtcgacatcg acgtcgtcgc gccggcgatc cagttgattg
     9541 gggcgatccc cgaggactgc atcgaactcc ttcatggcca cgggaacgag gtccgggtgt
     9601 gccacccagg tgccgtcgca ccccgaccca gcctcacgtg ccttgtcggc ggtcaccgca
     9661 gcgattgcct tttccgtgac ggaggcatcc cgccgattcg ggatgaacgc ggccattccc
     9721 ccaattgcgt gcgctcccct ctcgtggcag gtcttgacca tcagctcggt gtaggcgcgc
     9781 atgaagggaa ccgtcatcgt cacggatgag cgatccggca acacgaacga cgggtcgttc
     9841 cggaacttct tgatgaccga gaagatgtag tcccaccgtc cggcgttgag cccggccgca
     9901 tgctctcgaa gctcgtagag gatctcatcc atctcgaacg cggccaggat cgtctcgatc
     9961 aggacggttg ccttgatcgt gcctcgtggg atccccagcc ggtcctgtgt ccagacgaat
    10021 acatcattcc agagcctggc ctcgcgatgg gactccagct tgggcaggta gagatacggg
    10081 ccggtgccgg cttcgattgc tgcccgccct ccatgaaaca agtacaagcc gaagtcgaac
    10141 aatgaccccg acatcgggcg cccgtcgacc aggaagtgct tctcaccgag atgccaccca
    10201 cgaggacgga ccaacaaggt ggcggtctcg tcggcgagct cgtaggtctt ctccccggtg
    10261 tcgagtctca gctcccgccg gtaggcgtcg accagattcg cctggccctc gaggaggttg
    10321 gcccaggttg gagaagtcgc gtcctcgaaa tccgccatga acacgcgagc acccgagttg
    10381 agggcgttga tcatcatctt ggcgtccacc gggccggtga tctcgacacg gcggtcggag
    10441 agatcggcgg gaacgacatc gatcccccag tccgactccc tgatctcacg ggtctcgggg
    10501 aggaagtcgg gcttctcccc atcgtcgaga cgtcgttgcc tggtgtgtcg atcggcgagc
    10561 agctgtcctc gctgccagcc gaactcctgc tccagatcgg tgagaaacgc gaccgcctcc
    10621 ggggtgagca cccgtcgttc gatctcggta tctcgaaact gattcataag atggcagtgt
    10681 ctttaccgct agattacaat tccaaaccaa gtgagactaa agaatagccg atctttcggt
    10741 gggcgcgaga aatgacccaa tctcaacagc tagatcctct catcctcggt cgacggcttc
    10801 ggtatcaccg gagggaacgt gggatgactc tcgacgagct gggcgcggtc gtccatcgac
    10861 ccgcgcccta tctctccctc ctcgagaacg gcaagaaaca gccacgtctg aatctggtca
    10921 tcgagctcgc cgcggccctc gacgtcgaca ttgccgagct tctcgacccg agcccgccca
    10981 accgcagaga cgctttggag atcgcactgc tcagatcgca ggaatcccca tttttcgact
    11041 cgcttcacct cccctctgtc aagccgagcg ccaagctcga caacgagacg ctgactcatc
    11101 tggtcggtct gcacgacgcg ttgcgagagc gatccaacct gggatcggcg ggctccgagg
    11161 aggtgcggcg ggcgaacggg gaggtgacgt cgtggctcgc cgggaacgac ggctatctgg
    11221 aggaggtgga gaccgaggct cgaaatgcgc tcgcctcgag tgggtactca ggtgagggcc
    11281 cattttcgtc gcggaacctc ctcgacctga ccgccagcgc cggcttcgag ctgcaccctg
    11341 tcgaagacat gcccaccttc gccagatcga tcatcgacct cgaccaccat cgcgtctaca
    11401 tcgcccaacg agacgccttg cggacccgcc aggctcgcaa ggcggtcttg caaaccctcg
    11461 ccggcttcct gctcggtcac gagacggacc ccgatctgga tactttccta cgccagcggg
    11521 tcgagactgc ctacttcgcc gccgcggtgc tggcgccgga ggatcctgtc gtctccaggc
    11581 tgggtggggc gaaggacaac cacgacatcg atgtcgagga cgtcaaggag ctcttctaca
    11641 tctcatacga gatggccgcg tggcgaacag tgaacctggc cactcgccac ctcgacatca
    11701 cgacccatct gatcgtcagt gacgagatgg ggaccgtggt caaggcctac agcaatgacg
    11761 gggtgccgat cccacgggat gccaatggtg ggatggagac acagcgtctc tgccgacggt
    11821 ggggtgcccg catcactttc gactcggcgg agcgatatgg gacccaccag cagtacaccg
    11881 acacgccgga agggaccttc ttctgcacca cccatgtcga ggccggccga gacccggctc
    11941 atgccgtcac agtgggcgtc ccattctccg aagcccgatg gttccggggg cgggaaacaa
    12001 cgaaccggca aacctcgaca tgtcctgacc cgagttgttg cccgatggcg ccaccggccc
    12061 tccagcagaa atggtcggac aaggtcatcg tctcggctcg ctcccagaac cggatcctgg
    12121 gtctgctcgc tcccgaccca taccccgagc tggacatgcc cgaggtgctg gatctcgtgg
    12181 aggctcacgc agagagctga tcagcgagcg gtgtcctcga tgaaggctct caggtgaccg
    12241 gcgagggcga ccagcgactc acgttgcagg tacatcatgt gacccgcctc gtagtactcc
    12301 atctggatgt tcttgcgcaa tgactcgtcc aggccgagat ggctcacggt gtattcggtg
    12361 gcgtagtgcg gcgtggccag gtcgtagtac ccactggcga tgaacacctg cattcgctgg
    12421 gatcgggaca tgacgtcgcg cagctgctct gaggtgtcca cata
//
LOCUS       DASTXO010000106         2768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_890334, whole genome shotgun sequence.
ACCESSION   DASTXO010000106 DASTXO010000000
VERSION     DASTXO010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890334"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..961
                     /locus_tag="VFU96_02145"
     CDS             77..961
                     /locus_tag="VFU96_02145"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5112086.1"
                     /translation="MGSYVSTPRGPLHEVDFGGTGPNLILIHGLGGSTTNWNAVAPAL
                     TALGRVRAIDLPGFGLTPPRSDFRLETHRDSVVAYLDTIGGRFILSGNSTGGLLAELI
                     ASARPDLVDRLILVSPATPPVVPDPRLHWPTAARLAIQAMPGVGEAYGRRFVRSNTPE
                     QLVRKSLNMITHKPGRVPMSLIEESREMARIRKQLPWAEYATARTATSIARLYTRRPE
                     YLAMILAIEAPTLVVQGVTDHIVSPTAVEWLCSLRPDWDLVHLDDTGHTPQMDAPHRF
                     LDVVRNWLSRADQRVVGA"
     gene            1005..>2768
                     /gene="aceE"
                     /locus_tag="VFU96_02150"
     CDS             1005..>2768
                     /gene="aceE"
                     /locus_tag="VFU96_02150"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEU5112087.1"
                     /translation="MTGDLSRTLGDEYVHQMPDSDPVETQEWIDSLDAVVNEHGPDRA
                     KYLVAKLLERAHENNVGIAGAITTPYINTIPPEEEPEYPGDEHIERRIRRFIRWNAAV
                     MVIKANHRSKGIGGHLSTFASSATLYEVGFNHFFKGKGADGKGDHVYMQGHAAPGIYA
                     RAYLEGRFDEKHLDNFRFEIDGHGLSSYPHPRLMPDFWEFPTVSMGLGPINSIYHARF
                     NKYLHNRELDDTSKSRVWCFIGDGEVDEPETLGAISLATREGLDNLTWVVNCNLQRLD
                     GPVRGNGQIITELESVFRGAGWNVIKVIWGSGWDALLARDHEGALINVMNTTVDGEYQ
                     RYKAENGAYVRERFFGKDPRTKEMVADMSDDEIWALRRGGLDYKKVYAAYKVATELDN
                     GKPTVILTKTIKGWTLGPDVEGRNATHQIKELTNQQLMVLRERLSLQEEIPEESLAGE
                     SVPPYFRPEPGTPEHTYLHARRQELGGFLPSRVVPENTLVLPPPETFAEVYAGSGKVE
                     ASTTTAFTRLLRGLARSEGFGKHVVPIVPDEARTFGMDSLFRELKIYASKGQLYEPVD
                     ASMILSYSESQSGQILEEGITE"
BASE COUNT          524 a          928 c          864 g          452 t
ORIGIN      
        1 gtcccaactc gtaggtggag acgaggagca cacgcatcga gccagtgtac ggccaggccc
       61 cggtaccctt tcgtcgatgg gtagctacgt gagcacaccc cgtggaccat tgcacgaggt
      121 cgacttcggc ggtaccgggc caaacctgat cctgatccac ggcctgggag ggtcgaccac
      181 caactggaac gcggtggccc cggccttgac ggcactgggc cgggtgcggg ccatcgatct
      241 ccccgggttc ggcctcactc cgccgcggtc cgacttccgg ctcgagacgc atcgcgattc
      301 ggtggttgcg tatctcgaca cgattggtgg ccggttcatt ctcagcggga actcgactgg
      361 tgggctcctc gccgagttga tcgcgtcggc ccggccggac ctggtggatc ggctgatcct
      421 ggtgtcaccc gccactcctc cggtggtgcc cgacccgcgg ctccactggc ccaccgcggc
      481 tcgactggcc atccaggcga tgcccggcgt cggtgaggcc tatggccgcc ggttcgtgcg
      541 ctccaacacc ccggaacagc tggtgcggaa gagcctgaac atgatcaccc acaagccggg
      601 gcgcgttccg atgtcgctca tcgaggagtc acgcgagatg gcccggatcc ggaagcagct
      661 gccttgggcc gagtacgcca cggcgcggac ggcgacgtcg atcgcccgtc tctacacgcg
      721 gcgcccagag tatttggcca tgatcctcgc catcgaggcg ccgaccctgg tcgtccaagg
      781 ggtgacggac cacatcgtct cgcccacggc ggtcgaatgg ctctgctcat tgcggcccga
      841 ctgggacctg gtccacctcg acgacaccgg ccataccccg caaatggatg cgccgcaccg
      901 cttcctcgat gtcgtccgca attggctgag tcgagccgac caacgagtgg taggcgcctg
      961 acgccctggg gagagaaacc cctctgcaaa ctaggcttgg cccgatgaca ggcgatctga
     1021 gccgcaccct cggtgacgag tacgtccatc agatgccgga ttccgatccg gtggagaccc
     1081 aggagtggat cgattccctc gacgcggtgg tcaacgagca cgggcccgac cgcgccaagt
     1141 atctggttgc caagctgctc gagcgcgccc atgagaacaa cgtgggcatc gccggggcga
     1201 tcacgacgcc atacatcaac acgatccccc ccgaggagga gcccgaatac cccggtgacg
     1261 agcacatcga gagacggatc cggcgattca tcaggtggaa cgccgcggtg atggtgatca
     1321 aggccaatca tcgctccaag ggaatcggtg gccacctgtc cacgttcgcc tcctcggcca
     1381 ctctctacga ggtcggcttc aaccacttct tcaaggggaa gggcgccgat ggcaaaggcg
     1441 accacgtcta catgcaaggg catgcggctc ccgggatata tgcccgggcc tatctcgagg
     1501 ggaggttcga cgaaaagcat ctcgacaact tccgattcga gatcgatggc cacgggctct
     1561 cctcctatcc ccacccccgt ctcatgcccg atttctggga gttccccacg gtttcgatgg
     1621 gtctggggcc gatcaactcg atctatcacg cccggttcaa caagtacctc cacaaccgcg
     1681 aactggacga cacctccaag tcgcgggtct ggtgcttcat cggcgacggc gaggtcgacg
     1741 agcccgagac cctcggcgcc atctccctgg ccacccgcga gggcctcgac aacctgacct
     1801 gggtggtgaa ctgcaacctg cagcgcctgg acggcccggt gcgtggtaac ggccagatca
     1861 tcaccgagtt ggaatcggta ttccgcggcg ccggctggaa cgtgatcaag gtgatctggg
     1921 gctccggctg ggatgcccta ctcgcccgcg accacgaggg ggccctcatc aacgtgatga
     1981 acaccaccgt cgacggggag taccagcggt acaaggctga gaacggcgcc tatgtccgag
     2041 agcgcttctt cggcaaggac ccacgcacca aagagatggt cgccgacatg agcgacgacg
     2101 agatctgggc gttgcgacgc ggtggcctcg actacaagaa ggtgtacgcg gcctacaagg
     2161 tggcgaccga gctcgacaac ggcaagccga ccgtgatcct gaccaagacg atcaagggat
     2221 ggaccctggg ccctgacgtc gagggcagga acgccacgca tcagatcaaa gagctcacca
     2281 accaacagct gatggtgctg cgagagaggc tcagcctcca ggaggagatt ccggaagaat
     2341 ccctcgcggg tgagagcgtg cccccctatt tccggcccga gcccggaacg cccgagcaca
     2401 cgtacctcca cgccagacgt caggaactgg gcggattcct tcccagtcgt gtcgtacccg
     2461 agaacacgct cgtgctcccc ccgcccgaga ccttcgccga ggtgtacgcc ggttcgggca
     2521 aggtcgaagc ctcgacgacg accgccttca cccgtctgct gcgcggtctg gcccgttcgg
     2581 aggggtttgg caagcacgtg gtgccaatcg tccccgacga ggcgcggaca tttggcatgg
     2641 actccctctt ccgcgagctc aagatctacg cctccaaggg ccagctctac gagccggtcg
     2701 acgcgtcgat gatcctctcc tactcggaga gccagagcgg ccagatcctc gaggagggga
     2761 tcaccgag
//
LOCUS       DASTXO010000107         3920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1553556, whole genome shotgun sequence.
ACCESSION   DASTXO010000107 DASTXO010000000
VERSION     DASTXO010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553556"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2093)
                     /gene="pheT"
                     /locus_tag="VFU96_02155"
     CDS             complement(<1..2093)
                     /gene="pheT"
                     /locus_tag="VFU96_02155"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU5112088.1"
                     /translation="MRVPLRWLQEFIDLPTTDPMQLSDVLTMLGHEVESFDVLEPGWT
                     NVVVGRVEQIRPHPDADKVRVCQVETGSGPQQIICGAWNFSEGAHVAVAQPGAILPGE
                     FEIGRRTIRGVESNGMICSESELDLGEDHTGILVLDGEPVPGSPFVELVELPDVVFDV
                     AITNNRPDAMSLVGIARDLAAHYEIKHRVPERPLTTVPGSPAISVSIEDPSGCRRFTA
                     REIHGVEVGTSPLWVRQRLRKAGMRAISNIVDVTNYVMLELGHPLHAFDADTISGDRL
                     TVKRAETGETLETLDGEQRTLTSDDLIIYDTDGPTSMSGTMGGARSEVSESTVNVLME
                     AASWDPPTIMYMSRRHGLMSEASTRFERGVDPNLTDQANARAAAMVVELGGGTVLDGV
                     IDEVAVETRAVEIDLTLTDVERLLGTGFTTEQVARILTGLGMTVDGIDPMVVTVPSYR
                     PDVTRPADLVEEVARLHGYDRFGATLPTGPGGGLDPEQKRARLLTSALVGLGMHQAVT
                     LPFVNQADLAKLGWTETDRLLTVANPLREEEATMRPTMLPGLLNVARYNLSYGMAEVA
                     LFEMARVFLAEPWSEDPRLPAQRATLAWVIVGGFGPRLIGEEPPMADAVISISVLRHV
                     MAVLGHLEFGVEPAEGPGYHPGRSALVRLGGSVIGHVGELSPRATREFDMPGRVAIGE
                     VDLEPLLARVDAVIAT"
     gene            complement(2090..3139)
                     /gene="pheS"
                     /locus_tag="VFU96_02160"
     CDS             complement(2090..3139)
                     /gene="pheS"
                     /locus_tag="VFU96_02160"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEU5112089.1"
                     /translation="MDAISVLDGVLSEAPAAVDGATSLEELDRVESGLLGKSSPLGEV
                     RRSMGSLQPAERPLVGEKLNEVTGLVQDLLAARRIQLEIEDESLRLTEDRIDVTLASH
                     SFPQGSRHLLQQTIDEIVDIFNGLGYVMADGPEAELGWYNFDALNTPPTHPARLESDT
                     LYLDYGDGADEVLLRTQTSPVQVRWMETHDPPVYVVVPGKVYRQETTDATHLPVFHQI
                     EGLAVDESIQFSDLKGTLAHFSREFFGPTTSVRLRPDFFPFTEPSADLSVSCFACDGG
                     QPGCRVCGGAGWLEMLGCGMVDPNVLLAVGYDPSKVSGFAFGMGVERMAMVRHGINDI
                     RHFVDNDLRFLGQFR"
     gene            complement(3164..>3920)
                     /locus_tag="VFU96_02165"
     CDS             complement(3164..>3920)
                     /locus_tag="VFU96_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU5112090.1"
                     /translation="SHRNPRVVEAARLNRARVRRATGKTLLEGPGLLAAALDAGVTPE
                     VVFALPDDEMTGQLSERRDFELFVVESSVLRRVSGTETPRGPVAVITVPEPSRPTGGD
                     MIVAWGLGDPGNVGTLIRTAAAFGWDFGFTEGTADPWGPKVLRAGAGGHFRLAITPVQ
                     AVQSLEAAGLATVAAIVSGGANPADLGSGPFALLVGEEVPGLPPDVVEQASHTVTIPM
                     PGGMESLNAAMAAGIVVYELSKERTPGEECGER"
BASE COUNT          702 a         1316 c         1250 g          652 t
ORIGIN      
        1 gtggcgatca ccgcatcgac ccgagccagc aagggttcga gatcgacctc cccgatcgcg
       61 acccggcccg gcatgtcgaa ttcccgggtg gcgcgtgggc tcagttcacc gacatgaccg
      121 atgaccgacc cgccgagacg caccaaagcc gaccggcctg ggtgatagcc gggaccctca
      181 gccggctcga ccccgaactc gaggtgcccg agcactgcca tgacgtggcg aaggacggag
      241 atagagatca cggcgtcggc cattggaggc tcctcaccga tgaggcgtgg accgaatcca
      301 cccacgatca cccaggccag ggtggcccgc tgcgcgggaa gtcgggggtc ctccgaccac
      361 ggctcggcca ggaacacacg ggccatctcg aagagcgcaa cctcggccat cccgtaggag
      421 aggttgtaac gggcgacgtt gagcaatccc ggaagcatgg tcggccgcat cgtggcctcc
      481 tcttccctca gcggattggc gacggtgagc aatcggtcgg tttcggtcca tcccaatttg
      541 gcgagatcgg cctggttgac gaagggaagc gtcaccgctt gatgcatccc gagcccgacc
      601 agggccgacg tgagcagccg ggcccgtttc tgctcggggt ccaggccccc accgggaccg
      661 gtgggaaggg tggccccgaa acgatcgtat ccgtggagcc ttgccacttc ttcgaccagg
      721 tccgcaggcc gtgtcacgtc gggccgatag ctcggcaccg tcaccaccat cgggtcgatt
      781 ccgtcgacgg tcatccccag cccggtcaag attcgggcca cctgctcggt ggtgaacccg
      841 gtaccgagga ggcgctccac gtcggtcagc gtcagatcga tctcgacggc gcgggtttcg
      901 acggccacct catcgatcac gccgtcgagc acggtgccgc ccccgagctc gacgaccatc
      961 gccgccgccc tcgcgttggc ttgatcggtg agattggggt ccacaccccg ctcgaaacgg
     1021 gtggacgcct ctgacatgag cccgtgccga cgggacatgt acatgatcgt cggcgggtcc
     1081 caggaagcag cttccatcag gacattcacc gtcgattccg agacctccga gcgggctcca
     1141 cccatcgtcc ccgacatcga ggtcggccca tcggtgtcgt agatgatcag gtcatcggag
     1201 gtaagcgtgc gttgctcccc atcgagggtc tccagcgtct ctccggtctc ggctctcttg
     1261 acggtcagcc gatcccccga gatggtgtcg gcgtcaaacg catggagtgg atgaccgagc
     1321 tccagcatca catagttggt cacgtcgaca atgttggaga tggccctcat gccggccttt
     1381 cggagccgtt gtcgcaccca cagtggagag gtgcccacct ctactccgtg gatctccctg
     1441 gcggtgaatc ggcggcaacc tgacggatcc tcgatcgaga cagagatcgc cggcgacccg
     1501 ggaacagtgg tgaggggccg ctcgggaacc cgatgtttga tctcatagtg agcagcgagg
     1561 tcgcgggcga tgccgaccag cgacatcgca tcggggcggt tgttggtgat ggcgacgtcg
     1621 aagaccacgt cgggaagctc gaccagctcg acgaacgggg aacccggtac cggctcaccg
     1681 tccagcacga ggatcccggt gtggtcttca cccaggtcga gctcgctctc ggagcagatc
     1741 atcccgttcg actcgacacc cctgatcgtg cgtcggccga tctcgaattc gccgggaagg
     1801 atcgccccgg gctgtgccac cgcgacatga gcgccctccg agaagttcca tgcaccacag
     1861 atgatctgct gagggccgga accggtctct acctggcaca cccggacctt gtcggcatcg
     1921 ggatggggtc tgatctgctc gaccctgccc acgacgacgt tcgtccaccc cggctcgagc
     1981 acatcgaagc tctccacttc gtgcccgagc atggtgagga cgtccgacaa ctgcatcggg
     2041 tccgtggtcg ggaggtcgat gaattcctgc agccagcgga gcgggactct catcggaact
     2101 ggcccaggaa ccggaggtcg ttgtcgacga agtgacgaat gtcgttgatg ccgtggcgaa
     2161 ccatggccat ccgctccact cccatgccga aggcgaatcc ggacaccttc gacgggtcgt
     2221 atcccactgc gagcaggacg ttgggatcca ccattccaca gcccagcatc tcgagccagc
     2281 cagcgccgcc gcacacccgg cacccgggtt gaccgccgtc acaggcgaaa catgagaccg
     2341 agagatccgc actcggctcg gtgaagggga agaagtcggg tcggagcctc accgatgtcg
     2401 tcggcccgaa gaactccctc gagaaatggg ccagggtccc cttcaggtcg gagaattgga
     2461 tcgattcgtc gacagccaac ccctcgatct ggtgaaacac cgggagatgc gtcgcatccg
     2521 tcgtctcctg gcgatagacc tttcctggaa ccacgacata cacgggggga tcgtgggtct
     2581 ccatccaccg cacctggacc ggggacgtct gggtgcggag caggacctcg tcggcgccat
     2641 ccccgtagtc gagatagagg gtgtccgact cgagccgggc gggatgcgtc ggcggagtgt
     2701 tgagcgcgtc gaagttgtac cacccgagct cggcctccgg tccgtccgcc atcacatagc
     2761 caagcccgtt gaagatgtcc acgatctcat cgatcgtctg ctggagcagg tgcctcgatc
     2821 cctgggggaa ggagtgactc gccagtgtga cgtcgattcg atcctcggtg aggcggagcg
     2881 attcgtcttc gatctccagc tgaatcctgc gggcggccaa caggtcctga acgagcccgg
     2941 tgacctcgtt gagcttctcc ccgaccaggg ggcgctctgc cggctgcagg gatcccatgg
     3001 acctcctcac ctcgccaagt ggtgagctct tgcccagaag cccgctctcg acgcggtcga
     3061 gctcctcgag gctcgtcgcc ccgtctaccg cggcgggagc ctcggacagc acaccgtcga
     3121 gcaccgatat ggcatccatg tgggcggcag gttagaaggc tcgctaacgc tcgccgcact
     3181 cctcgcccgg tgtccgctcc ttggacagct catagacgac gattcccgcg gccatcgcgg
     3241 cattgaggct ctccatcccc cccggcatgg ggatggtgac cgtgtgagac gcctgctcga
     3301 cgacatcggg tgggagaccg gggacctcct ccccgaccaa gagcgcaaag ggccccgacc
     3361 ccaggtcggc gggattagcc cctccactca cgatcgcagc gacggtggcc aatccggctg
     3421 cttccagaga ttggactgcc tggaccggtg tgattgcgag gcgaaaatgc ccaccggcac
     3481 ccgcccgaag gaccttgggc ccccatgggt cggcggtgcc ctcggtgaac ccgaagtccc
     3541 agccgaaggc agcagcggtc cggatcaacg tccccacgtt ccccgggtca ccgagccccc
     3601 aggcaacgat catgtccccg ccggtcggtc gcgagggctc gggcaccgtg atgaccgcta
     3661 ccgggcctcg aggggtctcg gtgccactca cccggcgcaa taccgaggat tcgaccacga
     3721 acagctcgaa atctcgccgt tcggagagct gccccgtcat ttcgtcgtcg ggtagggcga
     3781 acacgacctc gggggtcaca ccggcatcca gtgcggcggc gagaaggccg gggccctcga
     3841 gaagtgtctt tccggtggcc cgacgcaccc gggcacgatt gagccgggcc gcttccacca
     3901 cccgagggtt gcgatggctg
//
LOCUS       DASTXO010000108         2324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_43075, whole genome shotgun sequence.
ACCESSION   DASTXO010000108 DASTXO010000000
VERSION     DASTXO010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43075"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1342
                     /locus_tag="VFU96_02170"
     CDS             131..1342
                     /locus_tag="VFU96_02170"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEU5112091.1"
                     /translation="MSDDIYIVDAVRTPTGRLGGQLAHVRPDDLAAHVVAAVLRRQPD
                     LDPMAIEDIYWGAANQAGEDNRNVARMAGLLAGLPIEIPGATVNRLCGSGMQAVISGA
                     HALAAGWGDVVVVGGSESMTRSPYVMAKPDRPFATGPPEVVDTVLGWRLVNPRMNELY
                     PPITLGMTAEVVAAKYDISRDDQDAFALRSHQLALAAVDAGRFDDEIEPIEAPIDSRG
                     KEKAMIEADEGPRADTSMEALAKLPPVFKEDGTVTAGNSSPMNDGAAALILATEAGLA
                     AHGLTPVARIVSSAAAGVHPDVMGIGPVPASEKAMHRAGVRPDNIDLIEINEAFASQA
                     VASIRLLEVDPDRVNVNGGAIAIGHPLGASGARILTTLTHELRRQGGRYGLATMCIGV
                     GQGISTVIERV"
     gene            1335..>2324
                     /locus_tag="VFU96_02175"
     CDS             1335..>2324
                     /locus_tag="VFU96_02175"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU5112092.1"
                     /translation="MSEARPGPLDGVFVVEFGNLIAAPYAAMLLADMGARVVKVEPPE
                     GDLARRFGPYQNGESTFFMTVNRGKESLAIDSKDWLSKRVLDKLVRKADVLVHNLRHG
                     AIDRMGLGEARARELNPRLVYASISAFGAEGPYAERSGVDLIFQGEAGMISITGEPKD
                     GPRKTATTVGDYVAGTNAAFAIAAALAERPRTGQRIDISLRDGLMAVQSTWNALAFAS
                     DTQPERTGTASPFLAPNQVFEAADAPFTLAIVSDRHFEMLAQSIGMPDLTAQFPNNEV
                     RMAKRAALGRKLTQVFKTEKADHWVSLLGEAGLPVGHVLTLTEALSDPQARHNE"
BASE COUNT          432 a          759 c          752 g          381 t
ORIGIN      
        1 gggtctctgc ggggctcgtt catctctatc gtcctcttga accgaactta ggactcgaag
       61 attccgtggg atatagggga tcccccccat ttgacccgga gcgattggcg agaatgtgaa
      121 ctgatcgaga atgagtgatg acatctacat cgtcgatgcg gtccgcaccc cgacgggaag
      181 gctcggtggg cagctcgcac atgtcagacc cgacgacctc gccgctcacg tcgtcgccgc
      241 agttctgaga cgtcaacccg atctcgaccc gatggcgatc gaagacatct actggggggc
      301 ggccaaccag gcgggtgaag acaaccgcaa cgtggcccgt atggcgggtc ttctcgccgg
      361 cctccccatc gagatcccgg gagcgaccgt caaccgcctg tgtggctccg ggatgcaggc
      421 ggtcatatcg ggggcgcacg ctcttgccgc cggctggggt gatgtcgtgg tggtcggcgg
      481 gtccgagtcg atgacacgct ccccctacgt gatggccaag cccgaccgcc cattcgccac
      541 cggccctccc gaggtggtcg acacggttct cggctggcgt ctggtcaacc cgagaatgaa
      601 cgagctctac ccaccgatca cgctcggcat gacggcggag gtcgtcgccg cgaagtacga
      661 catcagccgg gacgaccaag acgcgttcgc ccttcgcagc caccaattgg ctctggccgc
      721 tgtcgacgct ggtcgcttcg acgacgaaat cgagcccatc gaggcgccga tcgactccag
      781 ggggaaggag aaggcaatga tcgaagccga cgaagggcca cgagcggaca cctcgatgga
      841 ggccctggcc aagctccccc ctgtcttcaa ggaggacgga accgtcaccg caggcaactc
      901 gtcgccgatg aacgacggcg ctgcagcctt gatccttgcc accgaggccg gcctggctgc
      961 ccatggcctc acccctgtcg ccaggatcgt ctcgtccgcc gccgccgggg tgcaccccga
     1021 cgtgatggga atcggtcccg tgccggcgtc ggagaaagcg atgcaccgag ccggcgtccg
     1081 cccggacaac atcgatctca tcgagatcaa tgaagccttc gcctctcagg ccgtcgccag
     1141 catccgcctc ctcgaggtcg atccagaccg ggtaaacgtc aacggtggcg cgatcgcgat
     1201 cgggcacccg ctcggggcgt cgggagcgcg gatcctgacc acgctgaccc acgagttgcg
     1261 acgtcagggc ggccgctacg ggttggccac catgtgcatc ggggttggcc aggggatcag
     1321 cacggtcatc gagcgtgtct gaggcgcggc ccgggccact cgatggcgtc ttcgtcgtcg
     1381 agttcgggaa cctgatcgcc gcaccgtatg ccgccatgct gctcgccgac atgggggcca
     1441 gagtggtcaa ggtcgaacca cctgaaggcg acctggctcg aaggtttggg ccctatcaga
     1501 acggcgagtc gaccttcttc atgaccgtca atcggggaaa ggagtccctg gccatcgaca
     1561 gcaaggactg gctgtcgaag agggtgctgg acaagctggt ccgcaaggcc gacgttctcg
     1621 tgcacaacct gaggcatggc gcgatcgacc gcatgggttt gggtgaagcc cgggctcgag
     1681 agctcaatcc ccgcctcgtc tacgcatcga tctcagcctt cggggccgag ggcccctacg
     1741 ccgagcgttc gggggtggac ctcatcttcc agggtgaggc gggcatgatc tcgatcaccg
     1801 gagaaccgaa ggatggaccg cggaaaacgg ccacgacggt cggtgattac gtggccggga
     1861 caaacgccgc ttttgccatc gccgccgccc tggccgagcg cccgcgaaca gggcaacgca
     1921 tcgacatctc gttgcgtgac gggctgatgg cggtgcaatc gacatggaac gccctcgcct
     1981 tcgcctcgga cacgcagccc gagaggaccg gcaccgcaag tccctttctc gccccgaatc
     2041 aggtgttcga ggcggcagat gccccgttca ccctcgccat agtgtccgat cgccatttcg
     2101 aaatgctcgc ccagagcatc ggaatgccag atctcaccgc ccagttcccc aacaacgagg
     2161 tgaggatggc aaaaagggct gcgctgggga ggaagctgac gcaggtgttc aagaccgaga
     2221 aggcggacca ttgggtgagc cttctcgggg aagccgggct gccggtgggt catgtgctca
     2281 ccctcaccga ggcgctgtcc gaccctcaag cccggcacaa cgag
//
LOCUS       DASTXO010000109         2393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_522105, whole genome shotgun sequence.
ACCESSION   DASTXO010000109 DASTXO010000000
VERSION     DASTXO010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522105"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VFU96_02180"
     CDS             <1..507
                     /locus_tag="VFU96_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013732587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEU5112093.1"
                     /translation="TDDPVFAEARSGVEERVQRFISARGSRTVDWFHKELGKIMLEYC
                     GMSRSEPGLTKALSEIPALYEEFKTDVRILGVAGGLNQTLERAGRVEDFFELSQAMCL
                     DALRREESCGGHFREEHQSDEGEAMRRDDHFSHVSVWEWTGDPGQPALNIEPLTFETL
                     MPSTRSYK"
     gene            504..1262
                     /locus_tag="VFU96_02185"
     CDS             504..1262
                     /locus_tag="VFU96_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEU5112094.1"
                     /translation="MNLTLQIWRQEGPDAPGSFKIYEVAGISEEMSFLEMLDVLNERL
                     VNQGDEPVAFDHDCREGICGSCGVMIDGQAHGPQLGTATCQLHMRKFESGSTITVEPW
                     RAAGFPLVKDLVVDRSSFDRIIEAGGYITVDTGSAPDANLTPVPKAAADTAFDAAACI
                     GCGACVAACPNGAANLFTAAKISHLNLLPQGQAERWSRTEDMVEVMEQYFGSCTNHGE
                     CEKACPKSISIDFIAMMNADYLRAKIKNRRLIAQ"
     gene            1396..1974
                     /locus_tag="VFU96_02190"
     CDS             1396..1974
                     /locus_tag="VFU96_02190"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEU5112095.1"
                     /translation="MTTERRDERGATLVEAALVYALLFLALFAVVEFGLAFKDWLSVS
                     HAAREGARAGATYGDDPTADIQILRDVERTLAPAGIANGMNVRVFNAEVPGTGEDYSY
                     TPGSDCSDNTPVGLPPLQGCCDWSPCPEPFRDSYQVPGWDPSTRDVEAPDLDRIGVEV
                     QFMHQWLTNYFVPSSDFTTVTDFQIEPQVFES"
     gene            1971..>2393
                     /locus_tag="VFU96_02195"
     CDS             1971..>2393
                     /locus_tag="VFU96_02195"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HEU5112096.1"
                     /translation="MSGRPERGASLVEVGLVLPILILLAIGLSEIGFLVVDYLTVTNA
                     AREGARTGAAAANFPTADDLILDAVEEASCNLRFGDLEKVRIFKADGLGDPIAGSINE
                     YSPGGSLSCDSPGNALSCDNGCPWAASGRDREPPGLDVL"
BASE COUNT          430 a          795 c          751 g          417 t
ORIGIN      
        1 accgacgacc cggtgttcgc cgaggcgagg tccggtgtcg aggaacgggt gcagcgattc
       61 atctccgccc ggggcagtcg gaccgtcgac tggttccaca aggagctggg caagatcatg
      121 ctcgagtact gcggcatgtc ccggagcgag cccggtctca ccaaggccct ttccgagatt
      181 cccgccctgt acgaggagtt caagaccgac gtgcggatcc tgggagtggc cggagggctc
      241 aaccaaaccc tggagcgggc gggacgagtc gaggacttct tcgaactgtc ccaggcgatg
      301 tgtctcgatg ccctgcgtcg tgaggagtcg tgcggcgggc atttccggga ggagcaccag
      361 agcgacgagg gcgaggccat gcgccgcgac gatcacttct cccacgtctc ggtgtgggag
      421 tggaccggtg accccggcca gcctgcgctc aacatcgagc cactcacctt cgagacgctg
      481 atgccgtcga cacggagcta caagtgaatc tgacgctcca gatctggcgt caggagggac
      541 ccgacgcacc cgggtcgttc aagatctatg aagtcgccgg gatctccgag gagatgtcct
      601 ttctcgagat gctcgatgtc ctcaacgaac gtctggtcaa ccagggcgac gagccggtcg
      661 ccttcgatca tgattgccgg gaggggatct gcggctcctg cggtgtcatg atcgacggcc
      721 aggcccacgg cccccagctc ggcaccgcta cctgccagct gcacatgcgc aagttcgaga
      781 gcgggtcgac catcacggtc gaaccgtggc gggcggcggg gtttcccctg gtcaaggatc
      841 tggtcgtgga ccgcagctcg ttcgaccgca tcatcgaggc cggcggctat atcacggtcg
      901 acaccgggtc ggcccccgac gccaacctca ccccggttcc caaggccgct gccgacaccg
      961 ccttcgacgc cgcggcctgc atcggttgcg gggcatgcgt cgccgcctgc cccaatggtg
     1021 ccgccaatct cttcaccgca gccaagatca gccatctcaa cctcctgccc cagggtcagg
     1081 cggagcgttg gtcacgcacc gaggacatgg tcgaggtgat ggagcagtac ttcgggtcct
     1141 gcaccaacca cggagaatgc gagaaggcct gccccaagtc gatttcgatc gacttcatcg
     1201 ccatgatgaa tgccgactat ctcagggcga agatcaagaa ccgccggctc atcgcccaat
     1261 aggccgattc cggccccgcc agtatccccc ttgtcagcgg aaatcttcga cgtagcctga
     1321 cggaattcgg ggaaccactc tgggtaggtt ctgctcaccg aacgtggtcg ctgggaccaa
     1381 acgggatagg ggcaaatgac aaccgagaga cgtgacgaac ggggggccac cctcgtcgag
     1441 gcagcgttgg tctacgcact gctctttctc gcccttttcg ccgtcgtcga gttcggtttg
     1501 gccttcaagg actggctgtc ggtcagccat gccgcccgcg agggggcccg cgccggcgcc
     1561 acatatggcg acgatcccac ggccgacatt cagattcttc gagacgtcga gcgcaccttg
     1621 gcaccggccg gaatcgccaa cgggatgaac gtccgggttt tcaacgccga ggtgcccgga
     1681 acgggcgagg actactcgta cacgccggga agcgactgca gcgacaacac cccggttggc
     1741 ctgccaccgc tccagggatg ttgtgactgg agcccgtgcc cggagccgtt ccgtgactcg
     1801 taccaggtcc ccggttggga cccatcgaca cgggatgtgg aggcacccga tctcgatcgg
     1861 atcggagtcg aagtgcagtt catgcaccag tggctgacca actacttcgt cccttccagc
     1921 gacttcacca ccgtcaccga cttccagatc gagccacagg tcttcgagtc atgagcggtc
     1981 gacccgagcg aggggcctca ctggtagagg tcggcctggt cctcccgatc ctcatccttc
     2041 tcgccatcgg tctctccgag atcggcttcc tcgtcgtcga ctacctcacc gtgaccaatg
     2101 ccgccaggga aggagcccgc accggcgcgg ctgccgccaa cttccccacc gccgatgacc
     2161 tgatcctcga tgcggtggag gaggcctcgt gcaatctccg cttcggagat ctggagaagg
     2221 tgcgcatctt caaggccgac ggcctcgggg atccgatcgc gggctcgatc aacgagtact
     2281 cccccggtgg cagcctctcc tgcgactcgc ccgggaacgc cctgagttgt gacaacggct
     2341 gtccctgggc ggcgagcggc cgggatcgag agccacccgg gctcgatgtc ctc
//
LOCUS       DASTXO010000110         5410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_374691, whole genome shotgun sequence.
ACCESSION   DASTXO010000110 DASTXO010000000
VERSION     DASTXO010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374691"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="VFU96_02200"
     CDS             complement(<1..945)
                     /locus_tag="VFU96_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEU5112097.1"
                     /translation="MRWVRRILVGLLILAVVIVCFGFFLVRRSFPQVDGEIEAAGLEG
                     PVEIVRDGEGIPHIYAGNEHDLFFAQGYVHAQDRFWQMDFWRHIGAGRLSEMFGDSQV
                     DTDLFLRSLDFTGLAEQELAMLDTGQRAILEAYADGVNAFVGSRSPSQISLEYSILPL
                     QASGYEIEPWTPINTLTWAKVMSWDLSWNMLEEIDRAVLSEGMPVERVEQLYPDYPSE
                     HPVIVATDEAATTESPTTALPKDAMAALGSAGARARDVWAITGGGSAGIGSNNWVVGG
                     SHTESGLPILANDPHLAIQMPSIWYQNALHCTDPADACP"
     gene            1004..1444
                     /locus_tag="VFU96_02205"
     CDS             1004..1444
                     /locus_tag="VFU96_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112098.1"
                     /translation="MRIRMTILTVVLLLFVACGGGDAATTTTPSTVPSTTTTVAPTTT
                     PTTVATTSTLGETTTTGPEIDVTITAGEVSGPARVTVAVGDPVSIWVVSDVDAEIHVH
                     GYDLFLEATAGVPIEIALTADVPGIWEVELEETHVPLFALEVTP"
     gene            1441..2799
                     /locus_tag="VFU96_02210"
     CDS             1441..2799
                     /locus_tag="VFU96_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112099.1"
                     /translation="MNRRFGVVTFSTGAIVLLIPGVAMAHGIGGRLDLPVPLSYFIVG
                     AGVVLVITFIALAVLWPEPRLQEGPGHQAARLTVRRGWLLPSLGVIGLVLVIGQLLPP
                     LFGLETDPTRPTIAPVLVWVMFWLVVPFAGALIGDWYTDINPWRSLGQVLRVGRGERM
                     SVLDTLGIWPAAVVLVAFTWLELVNPDSGSPITLGMAALGYTLGLMAAMAYAGRETGL
                     AVFDAFTPYNRLISAISPLGRRDDGRLVWRGWLRALTVLPGWRGLPAFVVVAIGTVTY
                     DGASATESFRNVMGDFGQTMLGETLLLLGCVLAIGAAYSVASWAAARMVGGEWTTASV
                     AMRFAHTLVPIALAYALAHYLTLIIFEGQQLIAAVSDPFALGWDLFGTADRKIDFFIT
                     AAEPVWYAQVAVIVTGHVLGVILAHDRALGDFGKEAVRSQYAMLLLMVALTTLGLLIL
                     AG"
     gene            2796..2978
                     /locus_tag="VFU96_02215"
     CDS             2796..2978
                     /locus_tag="VFU96_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112100.1"
                     /translation="MNVIFAHYGGIDEIGVFVVPAVLAILALRWAERRAKRAAAEKDG
                     EPDMEAVSAEEGTDGD"
     gene            2968..3891
                     /locus_tag="VFU96_02220"
     CDS             2968..3891
                     /locus_tag="VFU96_02220"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEU5112101.1"
                     /translation="MATDGHEMAIPLITDPASAGVSGRSKAASYFALTKPRIIELLLI
                     TTVPSMVLAAGRWPGLWPVLATLIGGSLSAGGANALNNYVDRDIDQIMKRTRSRPLAR
                     HDVPPVNALVFGIALGVAGFAWLWVTTNLLAAAIATGALLFYVFVYTLRLKRTSTQNI
                     VIGGAAGAAPVLVGWAAVTGSLALPAWVLFLVVFYWTPPHFWALAIRYRDDYERAGVP
                     MLPVVAGVEATTRKMLLYTGLMVAISLLLVPVAGMRWIYLVAAIVLGAWFLWDTWRVY
                     RRPEDAMRLFTTSTVYLSALFGAVMLDVLVR"
     gene            3888..4724
                     /locus_tag="VFU96_02225"
     CDS             3888..4724
                     /locus_tag="VFU96_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112102.1"
                     /translation="MRRLVPGLLGLAILLAACGSDEVEPFAVVASSNGSIGIGEQRVL
                     FALVDPETDEFLASPDREASVTIRDENGSPLDTYPMEFIWTIPDVRGLYVAHVDIPEA
                     ATYQATIDAEGLATAGPAGFVAFDDPPLIEPGEDAPASETRTSADFPDLAVITSDPEP
                     DPAMYESSVASAVTSGAPSVIVFATPAFCASQACGPLLDQVKALRPDYPGVEFIHVEI
                     YEDLQVDSPDELTAVPATSEWGLPSEPWVFVVDGSGVVTSAFEGAASDAELTAAIDAV
                     SA"
     gene            4764..>5410
                     /gene="coxB"
                     /locus_tag="VFU96_02230"
     CDS             4764..>5410
                     /gene="coxB"
                     /locus_tag="VFU96_02230"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEU5112103.1"
                     /translation="MARSRHRLIRLGVLLGAALFIGSCSLFGSEDQPLTTFEPAGPFS
                     EQINNLFWPVFWIAVAVFILVQGAILVAVFLFRDRSGAKEAKQLHGSPKLEIAWTVIP
                     ALILAGIAVPTTAAVFELTECDPDAIEIEVIGHQWWFEYHYPEAEIDTANIMVIPAGQ
                     EVCAVMTSDDVLHNFWIPALNGKRYLVPGQVTLLRLQADEPGTDWGHCAEFCGLS"
BASE COUNT          817 a         1787 c         1736 g         1070 t
ORIGIN      
        1 cgggcaagcg tctgctggat cggtgcagtg gagcgcgttc tggtaccaga tcgaaggcat
       61 ctgtatggcc aggtgagggt cgttggccag gatgggaaga cccgattcgg tgtgcgagcc
      121 gcccaccacc cagttgttgg acccgattcc ggcggaacca ccgccggtga tcgcccacac
      181 atccctggcc cgggccccgg cagatccgag ggcggccatg gcgtctttgg gtagggcggt
      241 ggtcggtgat tcagtggtcg cggcctcgtc ggtggccacg atgacggggt gctcggaggg
      301 atagtcggga tacaactgct cgacacgctc gaccggcatc ccctcgctga ggaccgcacg
      361 atcgatctcc tccaacatgt tccagctcaa gtcccaggac atcactttgg cccaggtcag
      421 ggtgttgatg ggtgtccacg gctcgatctc gtatcccgag gcctggagcg ggaggatgga
      481 gtattccagg ctgatctgcg acggagatcg gctcccgaca aacgcattta cgccatcggc
      541 gtatgcctcg aggatggctc gctggccggt gtcgagcatc gccagctctt gctcggcgag
      601 accagtgaag tccagtgacc tgagaaacag atcggtgtcc acctgactgt ccccgaacat
      661 ctcggagagc ctgccggctc ctatgtgacg ccagaagtcc atctgccaga accgatcctg
      721 cgcatggacg tagccctggg cgaagaagag atcgtgctcg ttgccggcat agatgtgggg
      781 gattccctcg ccgtctctca cgatctccac tggtccctcg aggcccgccg cttcgatctc
      841 gccgtcgacc tggggaaatg agcggcgcac caggaagaat ccgaagcaga cgatgaccac
      901 ggcgaggatg aggagaccga ccaggattcg ccgcacccat cgcacgccgc gagcctatgc
      961 cgggaatcct cgattggttg gacagagatc tagcgttccg ccgatgcgca tcaggatgac
     1021 gatcctcact gtcgtgcttc tcctgttcgt ggcctgcggc ggaggtgacg ccgccacgac
     1081 cacgacaccc tcgacggtcc cttcgaccac gaccacggtg gcccccacga ccactcccac
     1141 gacagtggcc acgacgtcga ctctcggcga gaccaccacc accggcccag agatcgatgt
     1201 gaccatcact gcgggtgagg tctcaggccc cgctcgggtc acggttgccg ttggcgatcc
     1261 ggtatcgatt tgggtggtgt ccgatgtcga tgccgagatc catgtccacg gctacgacct
     1321 cttcctcgag gcaacagcgg gagtcccgat cgagatcgcc ctcaccgccg atgtgcccgg
     1381 gatctgggaa gttgagctgg aggagaccca tgtccccctg tttgcgctcg aggtgacgcc
     1441 ttgaaccgac gcttcggcgt cgtcaccttc tccacaggcg cgatcgtcct tctcatccca
     1501 ggggtggcga tggctcacgg gatcggaggg cgactcgacc tcccggtgcc gctcagctat
     1561 ttcatcgtcg gagcgggcgt ggtcctggtg atcaccttca tcgccctggc agttctctgg
     1621 cctgagcccc ggctccagga gggccccggt caccaggctg cgcggctcac ggtgagacgg
     1681 ggttggctct tgccctcact cggtgtgatc ggattggtcc tggtgatcgg gcagttgctt
     1741 ccccccctgt tcggtctgga gaccgatccg acacggccaa ccattgcccc ggtcctggtg
     1801 tgggttatgt tctggctggt agtccccttc gccggcgccc tcatcggcga ctggtacacc
     1861 gacatcaacc cgtggcgatc gctgggccag gtcctcagag ttggtcgcgg cgagcgaatg
     1921 agcgtgctcg acacgcttgg catatggcct gcggcggtgg tgctggtcgc gttcacctgg
     1981 ctggagttgg tcaaccccga ttcgggcagc cccatcactc tgggcatggc agcgctcggg
     2041 tacaccctgg ggctgatggc ggcgatggca tatgccggcc gggagaccgg cctggcagtg
     2101 ttcgatgcct tcacccctta caaccggctc atctccgcga tctcaccgct ggggagacgt
     2161 gacgacggcc gtttggtctg gcggggatgg ttgcgggccc tgacggtcct ccctggctgg
     2221 cgggggctac ccgccttcgt cgtcgtggcc atcgggaccg tcacctacga cggcgcttcg
     2281 gccaccgagt cattccgcaa tgtgatgggg gacttcgggc agaccatgtt gggggagacc
     2341 ttgctcttgc tcgggtgcgt cctggcgatc ggggccgcct actcggtcgc ctcgtgggcg
     2401 gccgcccgga tggtcggggg cgagtggacc acggcttcgg tggcgatgcg ctttgcgcac
     2461 accctggttc cgatcgcgct cgcctacgcc ctggcccact acctgacgct catcatcttc
     2521 gaaggccagc agctgatcgc cgcggtctcg gacccgttcg cactcggatg ggatctcttc
     2581 ggaaccgccg accgcaagat cgatttcttc atcaccgccg ccgagccagt ctggtacgcc
     2641 caggtggcgg tgatcgtcac cgggcatgtg ctcggtgtga tccttgccca cgatcgggcg
     2701 ctgggtgact tcggcaagga ggcggtccgc tcccagtatg cgatgcttct ccttatggtg
     2761 gccctgacca cacttggcct gctcatcctc gcggggtgaa cgtgatcttt gcgcactatg
     2821 ggggcatcga cgagatcggc gtgttcgttg tgcccgccgt gctcgccatc ctcgccctgc
     2881 gctgggcgga gcgcagggcg aagcgagcag cggccgagaa ggatggcgaa cctgacatgg
     2941 aagccgtgtc ggcagaagag ggcaccgatg gcgactgatg ggcacgagat ggccatcccg
     3001 ctgataaccg acccagcctc tgcgggcgtg tcgggtcgct ccaaggcagc ctcctatttc
     3061 gccctgacca agccccggat catcgagctg ctgctcatca ccacagtgcc gtcgatggtg
     3121 ctggccgcgg gccgctggcc ggggctctgg ccggtcctgg ccacattgat cggaggatcg
     3181 ctctcggccg gtggggccaa tgccctgaac aactacgtcg accgggacat cgaccagatc
     3241 atgaagcgga cccgttcccg gcctctggct cgacacgatg tgccacctgt caacgccctg
     3301 gtgttcggta tcgctctcgg tgtcgccggg ttcgcctggc tatgggtcac caccaatctg
     3361 ctcgcggcgg caattgccac tggcgccctg ctcttctatg tgttcgtcta caccttgcga
     3421 ctgaagcgga ccagcaccca gaacatcgtg atcggggggg ccgcgggtgc tgctcctgtg
     3481 ctggttggct gggccgcagt gaccgggtcg ctcgcgctac cggcgtgggt cctcttcctg
     3541 gtcgtgttct actggacgcc accacacttc tgggccctcg ccatcaggta ccgggacgac
     3601 tacgaaaggg caggggtccc gatgctcccg gttgtggcgg gggtcgaggc aaccacgcgc
     3661 aagatgttgc tctacaccgg gctcatggtg gcgatcagcc tgctcctggt accggtggcc
     3721 ggcatgcgct ggatctacct tgtcgccgca atcgtgctcg gcgcctggtt cctctgggac
     3781 acctggcgcg tctatcgccg cccagaggat gcgatgaggc tcttcacgac gtcgacggtc
     3841 tatctgtctg ctctctttgg tgcagtgatg ctcgacgtcc tggtgcgatg aggcgtttgg
     3901 ttccggggct cctcggactc gcgatcttgc tcgccgcttg cggctcggac gaggtcgagc
     3961 cgttcgccgt cgtcgcctcg agcaacggaa gcatcggcat cggtgagcaa cgcgtgctct
     4021 tcgccttggt cgacccggag acagacgagt tcctggcctc gccggaccgg gaggcctcgg
     4081 tgaccatccg tgacgagaac ggctcgccgc tcgacaccta cccgatggag ttcatctgga
     4141 ccatccccga tgtgcgcggc ctctatgtcg cccacgtcga tatcccggag gcagccacct
     4201 accaagccac gatcgacgcc gagggcctgg ccacggcggg gccagctggt ttcgttgcct
     4261 tcgatgaccc tcccctcatc gagccgggtg aggatgcgcc cgcctcggag actcgtactt
     4321 cggccgactt ccccgacctg gcggtcatca cctccgaccc cgagcccgac ccggcgatgt
     4381 atgaatcgag tgtcgcttcg gccgtgacct ccggggctcc gtccgtgatc gtgttcgcca
     4441 cccccgcctt ttgcgcttct caggcttgcg gccctctcct cgaccaggtc aaggcgctcc
     4501 gaccggacta ccccggggtc gaattcatcc acgtcgagat ctacgaggac ctccaggtcg
     4561 actcgcccga tgagctcact gcggtcccgg cgacgagcga gtggggactc ccgtccgagc
     4621 cttgggtctt cgtggtggat ggctctggtg ttgtcacctc ggcgttcgaa ggtgccgcct
     4681 ccgacgccga gctgaccgcc gcaatcgacg ctgtcagtgc ttagttggtc ggtggttagg
     4741 cgagtccgta tgatgccagg ccgatggccc ggtcccgtca ccgcctgatt cgccttggcg
     4801 tcctcctcgg cgccgccctc ttcatcgggt cctgctctct cttcggtagc gaggatcagc
     4861 cgctcaccac cttcgagccc gccggtccct tctccgagca gatcaacaac ctcttctggc
     4921 cggtcttctg gatagccgtg gcggtgttca tcctggtgca gggggcgatc ctcgtggctg
     4981 tgttcctgtt ccgggatcgc agcggcgcca aggaggccaa gcagctccat gggagcccga
     5041 agctggagat cgcctggact gtcatcccgg ccctcatcct ggccgggatc gcagtgccca
     5101 ccaccgccgc ggtgttcgag ctgaccgagt gcgatccaga cgcaatcgag atcgaggtga
     5161 tcggccatca gtggtggttc gaataccact accccgaggc ggagatcgac accgccaaca
     5221 tcatggtgat ccccgccggg caggaggttt gcgccgtgat gacctccgac gacgtccttc
     5281 acaacttctg gatccccgct ctgaacggaa agcgctatct ggtcccgggc caggtcaccc
     5341 tgctccggct tcaggccgac gagccgggca cggattgggg tcattgcgcc gagttttgcg
     5401 gtctctccca
//
LOCUS       DASTXO010000111         4573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1369524, whole genome shotgun sequence.
ACCESSION   DASTXO010000111 DASTXO010000000
VERSION     DASTXO010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369524"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VFU96_02235"
     CDS             <1..479
                     /locus_tag="VFU96_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112104.1"
                     /translation="YENEAADARAKEVTDALVELIPLWFGASPTPGEEGGTIWHDRHL
                     PGDLAGLVALVEKLQENDGPLVGLEMHSAAANTLDADAYDVGDLHTWQLLATIAGRPL
                     PEAMPPKMDLVEYYDRYVHPGIDELAERAGRENFDRDRARRRMLGDDAPPPQEYEQ"
     gene            491..622
                     /locus_tag="VFU96_02240"
     CDS             491..622
                     /locus_tag="VFU96_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112105.1"
                     /translation="MEEREVLVAFILDPDDTEEGLWEWLQVHCPYLFKSVSPKNEEG"
     gene            622..1173
                     /locus_tag="VFU96_02245"
     CDS             622..1173
                     /locus_tag="VFU96_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112106.1"
                     /translation="MLYAAVARNPMNDDPEGGHSDGYLPMVFVGPFKTEEEATTHGVA
                     VTIHPWTDEDESEGDHVVLWFGRYGTWGLFGCFKANEVAGFEYEDELTATHYLGESSV
                     SVELVDPEEFASNVQDFSSPDDDYERWTAHYVKCEAKEEGHWFVGYGWSTNESRADAY
                     DQARNYWDVSVDYLTIDRSKEIE"
     gene            1288..1599
                     /locus_tag="VFU96_02250"
     CDS             1288..1599
                     /locus_tag="VFU96_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112107.1"
                     /translation="MENRVDTARIKVQERIDRLSDDARAKLESTTDITFDEHFQFQET
                     QARFHAMEIINQEEALIVYGALGEVGSQENGGWAEGTDLATKIVVTQLMAELLALRLA
                     I"
     gene            1599..1766
                     /locus_tag="VFU96_02255"
     CDS             1599..1766
                     /locus_tag="VFU96_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112108.1"
                     /translation="MRPKRHRLNTDVRTWQDTRRQEAIDRIVRENPRFAPTDKPATVD
                     DHRLLKRFRRR"
     gene            1773..2048
                     /locus_tag="VFU96_02260"
     CDS             1773..2048
                     /locus_tag="VFU96_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112109.1"
                     /translation="MIVRLVDPQNREQRTLELPPGIIVTPECVSATGGDGNQDIATSQ
                     VIAAHSEGTLDIRVGWREVATGRIWPELFIHSEESIFSAAATQYEEV"
     gene            2053..2322
                     /locus_tag="VFU96_02265"
     CDS             2053..2322
                     /locus_tag="VFU96_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112110.1"
                     /translation="MADDRTEPVVIDVLGISPGQPQEEDAYNAITAAVTIAGEYAPLY
                     LVVDGKRHYVDRVIVTYEPDPAFGAEGMRNVIEIHGKEGGWTTRP"
     gene            2304..2756
                     /locus_tag="VFU96_02270"
     CDS             2304..2756
                     /locus_tag="VFU96_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112111.1"
                     /translation="MDDETMIGLGDFDSGDPNDEPGNPDEDEEMRPHKRWAVCPRCHG
                     NGSHVNPSIDGNGITESEMAELGEDFREDYMSGVFDVPCEECGGLRVVPGCHCGQPVV
                     AGHWYPGEIDEPEPYTLCHEHLEGEQLSQWRAMAELAAEQAAERRARC"
     gene            2750..3028
                     /locus_tag="VFU96_02275"
     CDS             2750..3028
                     /locus_tag="VFU96_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112112.1"
                     /translation="MLKQGDVVVVTVDGEPLGLVLEEFFPYEPANYVTDPCWLATTTG
                     GGAFTPPFVYSLNESGRVERAPIDKLHYIGKWELVGDHDLADPEYGGM"
     gene            3028..3345
                     /locus_tag="VFU96_02280"
     CDS             3028..3345
                     /locus_tag="VFU96_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112113.1"
                     /translation="MRVLANAVNVIFAEQRGPTSHDNFRVSATIYRDGLVNSKQMDEL
                     VEEEIVELRPVGPEDSEYFLTDYWRELLGPQIRTLHHDWFKVTPDPWKEGDQHETPTE
                     GGS"
     gene            3317..3673
                     /locus_tag="VFU96_02285"
     CDS             3317..3673
                     /locus_tag="VFU96_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112114.1"
                     /translation="MRRRQKEALRRMGIETENYYVNAWPPTWGIALMRDGEDFAVVES
                     HGDHVDEQNRHLRGKYVIRDRRATGPPHLPGGEWGDADLGAELGVYRRQGDALLAATQ
                     MRPDSRLTIRKREEGE"
     gene            3670..3870
                     /locus_tag="VFU96_02290"
     CDS             3670..3870
                     /locus_tag="VFU96_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112115.1"
                     /translation="MSEEAKILAQILAEMKGIRKEQKSIREGQEVQRLLILAMAEAMV
                     ANPSVFGQQNIAYNLQRIQEQT"
     gene            4133..>4573
                     /locus_tag="VFU96_02295"
     CDS             4133..>4573
                     /locus_tag="VFU96_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112116.1"
                     /translation="MATAEETATQVTEVPETETPEPAAEPTKPRAPRKPRKATGRTAK
                     PKSDKRKNKKSKYGGTVGAPRLGANGRRLTFYLIEEMGPEKANEILAAAIKRTLDKRP
                     HASRPKADRFIAWETIVAVAKIKDGEKLIRRLERQKGAKEQGLKD"
BASE COUNT         1023 a         1366 c         1461 g          723 t
ORIGIN      
        1 actacgaaaa cgaagcggcg gacgctcgcg ccaaggaggt gacggatgcc ttggtcgagt
       61 tgatcccgct gtggtttggc gcctcgccga caccgggcga ggagggcggc accatctggc
      121 acgaccgtca ccttccggga gatctggctg gtcttgtggc cttggtcgag aagctccagg
      181 aaaacgacgg gccgctcgtg gggttggaga tgcactccgc tgccgccaac accctcgacg
      241 ccgacgccta cgacgtgggc gatctccaca cctggcaact gctggcgacc atcgctggtc
      301 gtccgttgcc cgaggccatg ccgcccaaga tggacttggt ggagtactac gaccgctacg
      361 tgcatcccgg catcgatgaa ctagccgaac gcgccggacg cgaaaacttc gaccgcgacc
      421 gcgctcggcg gcggatgctg ggcgacgacg ccccgccccc gcaggaatac gaacagtagg
      481 aggtggattg atggaagaac gcgaagtcct ggtcgctttc atcctcgacc cggacgacac
      541 agaggagggg ttgtgggaat ggctgcaagt ccactgcccc tacctcttca agtcagtgtc
      601 cccgaagaac gaggagggct gatgctctac gcagccgtag cacgcaaccc gatgaacgac
      661 gaccccgaag gtgggcattc cgacgggtac ctgcccatgg tgttcgtcgg ccccttcaag
      721 acggaggagg aggcgacgac tcacggggtc gccgtcacca tccatccgtg gaccgacgag
      781 gacgagagcg agggggatca cgtcgttctg tggttcggca ggtacgggac ctggggcctc
      841 ttcggatgct tcaaggcgaa cgaagtcgcc gggttcgagt acgaagacga actcaccgcc
      901 acgcattact tgggcgaatc ttcggtgagc gtcgaactgg tcgatcccga ggagttcgca
      961 agcaacgtcc aagacttctc ctcccccgac gacgactacg aacggtggac tgcgcactac
     1021 gtcaaatgcg aggcgaagga ggaaggccac tggttcgtcg ggtacgggtg gtctacgaac
     1081 gaatcccgag ccgacgcata cgaccaagcc cgcaactact gggacgtatc cgttgactac
     1141 ctcacgatcg accgctccaa ggagatcgag taagccccgg ctgaatccga agaacactac
     1201 cgggttgacg gttggcactc accgtgatat cgttcgtggt gtcacggaat caccccgccc
     1261 agccggggaa tgagaggagg tgtaggcatg gaaaatcgtg ttgacacagc acgcatcaaa
     1321 gtgcaggaac gcatcgaccg tctgtcggac gacgcacggg cgaagctcga gtccaccacg
     1381 gacatcacct tcgatgaaca cttccagttc caggagacgc aggctcggtt ccacgccatg
     1441 gagatcatca accaggagga ggcgttgatc gtgtacggcg ctcttgggga ggtgggctcg
     1501 caggagaacg gcgggtgggc agagggcacc gatctggcca cgaagatcgt ggtcacccag
     1561 ttgatggccg agcttctcgc tcttcgactg gcgatctgat gcgccccaag cgccatcgcc
     1621 tcaacaccga cgtccgcacc tggcaggaca cacgccgcca ggaagcaatc gaccgcatcg
     1681 tgcgggagaa cccgcgcttt gcaccaacgg acaagccagc aacggtggac gaccaccgtt
     1741 tgctcaaacg attcagaagg aggtgaagca cggtgatcgt aaggttggtc gatccgcaaa
     1801 acagggagca gcgcacgctg gaactcccgc ccggcatcat cgtcacgccg gagtgcgttt
     1861 ctgcaactgg cggcgacggc aatcaggaca tcgctaccag tcaggtcatc gccgctcaca
     1921 gcgagggcac tctggatatc agggtggggt ggcgcgaggt agcaacgggt cgcatctggc
     1981 ccgaactctt catccactcg gaggagtcca tcttctcggc ggctgccacc caatacgagg
     2041 aggtgtagca ccatggccga cgacagaaca gaaccggtcg tgatcgacgt gctgggtatc
     2101 tcgcccggtc aaccgcagga ggaggatgcg tacaacgcca tcacggcagc cgtcacgatc
     2161 gcaggcgagt acgccccgct ctatctggtg gtggatggca agcgccacta cgtcgatcgg
     2221 gtgatcgtga cctacgaacc cgaccctgcc ttcggcgccg aagggatgcg gaacgtcatc
     2281 gagattcacg gaaaggaggg aggatggacg acgagaccat gatcgggctc ggcgacttcg
     2341 actccggcga cccgaacgac gagccgggca accccgacga agacgaggag atgcgtcccc
     2401 acaagagatg ggccgtgtgc cctcgctgcc acggcaacgg gtctcacgtc aacccctcca
     2461 tcgacggcaa cgggatcacc gagtccgaga tggcggaact cggcgaggat ttccgcgagg
     2521 actacatgag cggagtcttc gacgtgccct gcgaggaatg cggtggcctc cgggttgtcc
     2581 ccggctgcca ttgcgggcag cccgtggtgg ctgggcattg gtaccccggt gagatcgacg
     2641 agccggagcc gtacaccctc tgccacgaac atctcgaggg tgagcagctg agccaatggc
     2701 gtgcgatggc cgaactcgcc gccgagcaag ctgctgagag gagggcgagg tgctgaagca
     2761 aggagacgtc gttgtcgtca cggtggacgg cgaacctctc gggctggtgc ttgaggagtt
     2821 cttcccctac gagcctgcca actatgtgac tgacccgtgt tggcttgcga ccacaactgg
     2881 tgggggagcg ttcactcccc cattcgtcta ctcgctcaac gaatccgggc gggtggaacg
     2941 agcgcccatc gacaagctgc actacatcgg caaatgggaa ctggtcggcg accacgatct
     3001 ggccgacccc gagtacggag ggatgtgatg agggttctcg ccaacgcagt caacgtgatc
     3061 ttcgccgagc agcgaggccc gacgtcccat gacaacttcc gcgtgtcggc gaccatctac
     3121 cgggatgggc tggtcaactc caagcagatg gacgaactgg tcgaagagga gatcgttgaa
     3181 ttgaggcccg tcggccccga agacagcgag tactttctca cggactattg gcgcgaactg
     3241 ctcggaccac agatccgcac cctccaccat gactggttca aggtcacgcc tgatccatgg
     3301 aaggaggggg accagcatga gacgccgaca gaaggaggct cttaggagaa tgggcattga
     3361 aaccgaaaac tactacgtca atgcatggcc tccgacttgg ggcattgcgc tcatgcgtga
     3421 cggcgaagat ttcgctgtgg tcgaatcgca cggcgaccac gtagacgaac agaatcgcca
     3481 ccttcgcggc aagtacgtga tccgggaccg ccgtgcaaca ggcccgcccc atctacccgg
     3541 cggtgagtgg ggggacgctg atcttggggc agagctgggt gtttacagga ggcaaggtga
     3601 tgccctcctc gctgccaccc agatgagacc ggattcacga ctgactatca gaaaaagaga
     3661 ggagggggaa tgagcgaaga ggccaagatt ctcgcccaga ttctcgccga gatgaagggc
     3721 atccgcaagg agcagaaaag catccgcgaa gggcaggagg tccaacgctt gctcattctg
     3781 gccatggctg aagccatggt cgcaaacccc tcggtcttcg gacaacagaa catcgcttac
     3841 aacctccaac gaatacagga gcaaacttag agctcagctc gcagaacacg actactaaca
     3901 gggaaaggcg attaggtcgg ccagctcatg tgctgcggtt ccctccgaaa gcaaactcac
     3961 ctggatagcc tcgtgttctg cgaggtgggc ttcaagcccc cccaaaggta tggaccgaga
     4021 aaatcaggtc aaattcgagt tgacaacggc ctgtcggtca tgtaccgttt ggacatacgg
     4081 aaaccggcgg taagaattcc gccacaaaag cacgaaagga ggtgtatgca ctatggcaac
     4141 cgcagaagag accgcaacgc aggtcaccga ggtcccggag acggagaccc cggagccggc
     4201 cgctgagccc acgaagcctc gggctccgcg caagccgcgc aaggccacgg gtcgcaccgc
     4261 caagccgaag tcggacaagc gcaagaacaa gaagtccaag tacggcggca cggtgggcgc
     4321 accccgcctc ggcgccaacg ggcggcgtct cacgttctat ctgatcgagg agatgggtcc
     4381 cgagaaggcc aacgagatcc ttgctgccgc gatcaagcgg acgttggaca agcggcccca
     4441 cgcctcgcgg ccgaaggccg accgcttcat cgcctgggag acgatcgtgg cagtcgccaa
     4501 gatcaaggac ggcgagaagc tgatccgtcg gctcgagcgg cagaagggcg cgaaggagca
     4561 gggtctgaag gac
//
LOCUS       DASTXO010000112         5015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_190549, whole genome shotgun sequence.
ACCESSION   DASTXO010000112 DASTXO010000000
VERSION     DASTXO010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190549"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VFU96_02300"
     CDS             <1..381
                     /locus_tag="VFU96_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112117.1"
                     /translation="TMLEVDPIQPATARKWLRLDLETGLCLMNVMFSLLTTSPEYRRA
                     VHSLQLDESVLAILAELTGDQVLRSWKPAGYIPVVRTNRDVPRDLFAHAVIHEYIHAR
                     LREHARRCGDDDVCPALEPVLTSI"
     gene            421..1560
                     /locus_tag="VFU96_02305"
     CDS             421..1560
                     /locus_tag="VFU96_02305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5112118.1"
                     /translation="MALASEGSPHFLPVDRTLTRVVILMRMLGWAWMVILIMVAMFIA
                     EPEPDVPILVGALLLGTAGTALTLLAASRGFLGEIWYVVLDGAIALLLVSAGWFAGTD
                     FISGGYPASWLFIVAFATNFRATIVIGAVAAAVFALLHIVMDLDQTRLFGSVQFLVVA
                     VVVGWAFDALRERERLRIHAEEERAQAEADLAREKERSMRLEERSQIARRLHDSVLQT
                     LKLISDTSEDPAEVRYLARVQERDLRQTINEYQSHHEDSYRARLLDVRATVEDRYRVE
                     IEQVIRDDAEMNDALRAVVAATSEALNNAARHSGMRQIDLFSEIRDGVAQVNIRDRGV
                     GFDPETVARGGLIDSIVSRVETAGGTAQVRSEPGKGTEVEIRVPL"
     gene            1560..2216
                     /locus_tag="VFU96_02310"
     CDS             1560..2216
                     /locus_tag="VFU96_02310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5112119.1"
                     /translation="MNDPSKPRVFLVEDHPLTLFGLQRYLEDRYDVVGSATEAAAAIE
                     MILERRPELVLLDVHFPGGGGPAVIEAVKKTDDEIKFLAFTVSTSAEDVVRLFKAGVD
                     GYVVKTTDGLALEEQIELALSGGRPVSRYVAGHLLSIDEVVSTQSEIERLTPKEREVV
                     TLIARGFRYREAADELGISQKTLETHMRHVFDKLGVASRHEMTRMAFETGFLRPGDFD
                     "
     gene            2274..3617
                     /locus_tag="VFU96_02315"
     CDS             2274..3617
                     /locus_tag="VFU96_02315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEU5112120.1"
                     /translation="MSTYANALEMARETPKTRNRYVDLLRAFSILVVVVGHWLMAAPQ
                     VVGDGVAFNHLLSTTGWTHYLTWILQVMPLFFLVGGYANAASWRSAQLRAEPYGAWLR
                     ARLRRLVLPVLPLLAVWTVGAYALFQSGLDPDLIRMGSQAALVPLWFLATYLAVVALT
                     PVTLRAWDRFGFASLILTAALAGLMDLLSLGFGFVAAGYLNYLFVWGTVHGLGYAWAD
                     SRLGGVTHRLILSAVGLITTAALVVFGPYPVAMVGLDTEAVTNSQPPKVTLIALALFQ
                     TGLFLAADGPLRRWLARDRVWAPVVLVNGRIMSIYLWHMTAMVLVIGGLMLAGGFGLG
                     VDVDSTMWWLTRPLWLAALAVVTLPFISGFGRFERPREDRRPAPSVWRPLVAVVLFCA
                     GLGFVAATGVVDDQGLNGLVLSLPFIAAIAGGVGGARHWEQKLDSMTGRARSDRV"
     gene            complement(3603..4535)
                     /locus_tag="VFU96_02320"
     CDS             complement(3603..4535)
                     /locus_tag="VFU96_02320"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEU5112121.1"
                     /translation="MARIFISYRREDSSGHAGRLYDRLRARFGDDVFMDIDAIGPGVD
                     YARLIDETIDTVEAVIVVIGQQWLSSTDAEGSRRLEDPKDLVRQEISAALARDVLVIP
                     VLVQGAVLPEPEALPPELAGLARHNAFEVSDARWNYDADRLVKALEESLATTAPAAPP
                     PVIDSGVDEGTSVTSAAKPSSTPMILAITGALLVLLFGLFVTPTWHDEQVWFRIVVAL
                     AVVGITSAGLITKKWSWVMIAGSVGLGGFVIWVLMLIGGHELSELTSFGTDGVTNLFF
                     LVGSALVLAGGWLGARSPSGQPPPARATTASDSV"
     gene            complement(4646..4987)
                     /locus_tag="VFU96_02325"
     CDS             complement(4646..4987)
                     /locus_tag="VFU96_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005575223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEU5112122.1"
                     /translation="MARVEHDLSEEQWTTLKAAWGGCAYCGATDRPLQRDCVLAISRG
                     GRYTLDNIAPACGPCNASKNNYEVTGWMRRRRFDESAFLLRHLEINAALALQFPADLN
                     SELDFRPTPHH"
BASE COUNT          844 a         1597 c         1602 g          972 t
ORIGIN      
        1 accatgctcg aggtggaccc catccagccc gcgaccgccc gcaagtggct tcgtctcgac
       61 ctggaaaccg gtctctgcct gatgaacgtc atgttctccc tcctcaccac cagccccgag
      121 taccgcaggg cggtgcactc gctccagctc gacgagtcgg tgcttgccat cctcgccgag
      181 ctcaccggtg accaggtcct tcgcagctgg aagccagccg ggtacatacc cgtcgtccgc
      241 accaaccggg acgtgccccg cgatcttttc gcccatgcgg tgatccacga atacatccat
      301 gcccgactcc gtgagcacgc tcggcgctgc ggagacgacg atgtctgccc ggcactggaa
      361 cctgtgctga cctcgatctg agaacggtgg atcgagaggt gatcccgata gcctctcttt
      421 gtggccctag ccagcgaggg cagtcctcac ttcttaccgg tcgaccgcac cctgaccagg
      481 gtggtcatcc tgatgaggat gctcggatgg gcctggatgg tcatcctgat catggtggcg
      541 atgttcatcg ccgagccgga accggatgtc cccatcctgg ttggagcgtt gctgctcggt
      601 acggccggga ccgccttgac cctccttgct gcatcgcgcg gattcctcgg tgagatctgg
      661 tacgtcgtgc tcgacggtgc catcgccctg ctcctggtct cggcgggatg gttcgccggc
      721 accgacttca tctcaggcgg ctacccggct tcgtggctgt tcatcgtcgc cttcgcaacc
      781 aactttcgtg ccacgatcgt catcggcgct gtcgctgctg cggttttcgc cctactccac
      841 atcgtgatgg acctcgacca gacgaggctg ttcggaagtg tccagttcct ggtggtcgcg
      901 gtcgtcgtcg gctgggcctt cgacgcgctg agggagaggg agcggcttcg catccacgcc
      961 gaggaagagc gggcgcaggc cgaggcggac ctggcaaggg agaaggaacg ctcgatgcgg
     1021 ctcgaagaaa ggtctcagat cgcccgccgg cttcacgatt cggtcctgca gacgttgaag
     1081 ttgatcagcg acacctcgga ggatcccgcc gaggttcgct atctggcccg ggtccaggag
     1141 agggacctac gccagacgat caacgagtac cagtcgcacc atgaggacag ctatcgggca
     1201 cggctcctcg acgtgcgggc caccgtcgaa gaccgttacc gggttgagat cgagcaggtg
     1261 atccgggacg acgccgaaat gaatgatgcg cttcgcgccg tggtggcggc cacctcggag
     1321 gccttgaaca acgcagcccg gcactctggg atgcgtcaga tcgacctgtt ctccgagatc
     1381 cgcgacggcg tggctcaggt gaacattcgt gaccgtggtg tcggcttcga cccggagacg
     1441 gtggcacggg gaggactgat cgattcaatc gtctcccggg tggagaccgc agggggaacc
     1501 gcccaggtca gatcggaacc gggcaaaggc accgaagtcg agatccgggt gcccctgtga
     1561 tgaacgaccc atcgaagcca cgggtgttcc tggtcgagga ccacccgctg acacttttcg
     1621 ggctgcagcg ctacctcgag gatcgttacg acgtggtggg gtcggccacg gaggccgcgg
     1681 ctgcgatcga gatgatcctg gagcgtcgtc ccgagttggt cctgctcgat gtccacttcc
     1741 ccggcggggg cgggccggct gtgatcgagg cagtgaagaa gacagatgat gagatcaagt
     1801 tcctcgcatt caccgtctcc acctcggccg aggacgtggt ccggttgttc aaggccggtg
     1861 tcgacggtta tgtggtgaag accaccgacg gcctggcgct ggaggagcag atcgagctgg
     1921 ctctgagcgg agggcgtccg gtctcccgct acgtcgccgg ccacctgctc agcatcgacg
     1981 aggtggtttc cacccaaagc gagatcgaaa ggctcacccc taaagagcga gaggttgtga
     2041 cgttgatcgc gcgcggtttt cgctaccggg aggcggccga tgaattgggg atcagccaga
     2101 agacgctgga gacccacatg cgccatgtct tcgacaagct gggggtcgcc agccggcatg
     2161 agatgaccag gatggcgttc gaaaccggat tccttcgacc cggggacttc gactgatccg
     2221 gatcggggta tatcccgata ccccgaattg cgtccctgtc cgatcctgtg cccatgagca
     2281 catacgcaaa cgcgttggaa atggcacggg agaccccgaa gactcgtaat cgctacgtcg
     2341 acctgttgcg ggctttctcg atcctggtcg tggtggtggg acattggctg atggcggccc
     2401 cgcaggtggt cggcgatggc gtcgccttca accatctctt gtcgacgacc ggctggaccc
     2461 actatctgac ctggatcctc caggtgatgc ccctcttctt cctcgtcggt gggtacgcca
     2521 atgcggccag ctggcgatcg gcccagctcc gggcagagcc atacggggcc tggctgcggg
     2581 ccagattgcg acgtctcgtc ctccccgtgc ttcctctcct cgcggtgtgg accgtcggcg
     2641 cctacgccct gttccagtca ggtctcgacc cggacctgat caggatgggc tcccaggcgg
     2701 cgttggtccc gctctggttc ctcgccacct accttgcggt tgtcgcgctg acaccggtga
     2761 cgctgcgtgc ctgggatcga ttcggattcg cttccctgat cctgacggcg gcgctggccg
     2821 ggctgatgga cctgctctcg ctcgggttcg gcttcgtggc cgccggatat ctgaactacc
     2881 tgttcgtctg ggggacggtc cacggcctcg gatacgcatg ggccgattcg cggctcggtg
     2941 gcgtcaccca ccgcctgatt ctgtcggctg tcggcctcat cacgactgcc gcgctggtcg
     3001 tctttggccc ctacccggtg gcgatggtcg gtctcgacac cgaggctgtg accaacagcc
     3061 agccgccaaa ggtgaccttg atcgctctgg ccctgttcca gaccggtctg ttcctggcgg
     3121 cggatggccc gctccgtcgt tggttggccc gggaccgggt gtgggcaccc gtggtgttgg
     3181 tcaacggtcg gatcatgagc atctatctgt ggcacatgac ggcgatggtg ctcgtgatcg
     3241 gtggtctgat gctcgccggt ggcttcggtc tcggcgtcga cgtggactcc acgatgtggt
     3301 ggctgacccg gccattgtgg ctggcggcgc tggctgtagt caccttgccg ttcatctcgg
     3361 gcttcggccg attcgaacgt ccacgagaag atcgccggcc ggcgccaagc gtctggagac
     3421 cgctcgtggc cgtggtgctg ttctgcgccg gccttggttt cgtcgccgcc accggagtgg
     3481 tcgacgatca gggtctgaat ggcctcgtcc tctcactgcc cttcatcgcc gccatcgccg
     3541 gcggcgtcgg cggtgctcgc cactgggagc agaagctcga ctcgatgacc gggcgggccc
     3601 gatcagaccg agtctgaggc tgtcgttgcc cgtgctggtg gtggttgtcc cgaaggcgag
     3661 cgggcgccca accaaccgcc ggcgagaact agcgccgatc caaccaggaa gaaaaggttg
     3721 gtgacaccgt cagtcccaaa cgacgtcagc tccgaaagtt cgtggcctcc gatcagcatc
     3781 agcacccaga tgacgaaacc ccccaacccg acactgccgg cgatcatgac ccaactccac
     3841 ttcttggtta tgagaccggc tgaagtgatc cccaccacgg ccagcgctac gacgatgcga
     3901 aaccacacct gctcgtcatg ccaggttggt gtcacgaaaa gtccgaacaa cagcaccagc
     3961 agggcgcccg tgatagccag aatcatcggc gtcgaacttg gttttgcagc cgaagtcacc
     4021 gaagttccct cgtcgacacc cgaatcgatc accggcggcg gcgccgctgg tgccgttgtg
     4081 gcgagcgact cctcgagcgc cttgaccagg cgatccgcgt cgtaattcca acgcgcatcg
     4141 ctcacctcga aagcgttgtg acgagccagg ccggcgagct ccggtggtag ggcctcgggt
     4201 tcgggaagga cagcaccctg caccaggacc ggtatcacca ggacgtcacg agccagcgca
     4261 gcggagatct cctggcgaac caggtccttt gggtcttcga gtcgacggct gccttcggcg
     4321 tctgttgagg agagccattg ctgtcctatg acaacgatca ccgcctcgac ggtgtcgatg
     4381 gtctcatcga tcagccgggc gtaatcgact cctggtccga tggcatcgat gtccatgaac
     4441 acatcatcac cgaaccgggc tcgaagtcga tcgtagaggc gacccgcgtg acccgagctg
     4501 tcctccctcc gatagctgat gaagattcgg gccatggatg cccccctcgc gttgagtcgg
     4561 tccttatggc gagttcgaca cggttaggac cgatcagccg cgtcagccag agagtcccta
     4621 ggggctcgaa gctaccacgc gcccctcagt ggtgcggggt gggacggaag tcgagttcgg
     4681 aattcagatc ggcaggaaat tgcaatgcca gagccgcatt gatctcgaga tgtcggagca
     4741 aaaaggcgct ttcgtcgaac cgtctccgcc gcatccaacc ggtcacttca tagttgttct
     4801 tgctggcgtt gcacgggccg caggccggag cgatgttgtc gagggtgtaa cgaccgccgc
     4861 gggagatcgc cagcacacaa tcgcgctgga gcggcctatc ggtggcaccg caatacgcgc
     4921 aaccacccca ggccgccttt agggtcgtcc actgctcttc gctcaggtca tgctcgacgc
     4981 gagccatgcg gcgcttgcgt ctgcgggcgc tcctc
//
LOCUS       DASTXO010000113         3151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_190619, whole genome shotgun sequence.
ACCESSION   DASTXO010000113 DASTXO010000000
VERSION     DASTXO010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190619"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VFU96_02330"
     CDS             complement(<1..457)
                     /locus_tag="VFU96_02330"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU5112123.1"
                     /translation="MISTNDVKPGMALQLSDGLFTIVEYQHVKPGKGRAFVRMKLKNL
                     ETGQVLDRTFRADENVDQAIIERRDHQYLYRDDLGFHFMNLEDFGQLALSGDDVGAAA
                     GYLVEGMTASLSLYRGTPIAVELPAAVELEVTYAEPAVKGDTRTGATKPV"
     gene            complement(503..1528)
                     /locus_tag="VFU96_02335"
     CDS             complement(503..1528)
                     /locus_tag="VFU96_02335"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HEU5112124.1"
                     /translation="MDALIVAGKTELVVGRGLPDPILPARSDRERVAVVTQPGATHIA
                     LDVGRQLQDAGLQIEVIGLPDREEAKTLVVAGSVYEALARFGLGRHDTIVGVGGGSVS
                     DLAGFVAGTWLRGVEVVHVPTTLLAAVDAAIGGKTGVNLAGKNLVGLFWHPTRVIVDV
                     NTLEDLPTTLRREGLAEALKTGLVGDRDLFELLEENGEEASLEEVVTKAAAVKVRVVG
                     EDERDSGLRAILNFGHTIGHAVEYASPLSHGESVAIGMVAAGRISERRLRFQGLDRMA
                     AAISGLGLPTRVDGLERARVEDLLRHDKKRDSSGMRMVLLRAIADPVIEHVEAADLDL
                     GLDAVGL"
     gene            complement(1516..2019)
                     /locus_tag="VFU96_02340"
     CDS             complement(1516..2019)
                     /locus_tag="VFU96_02340"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU5112125.1"
                     /translation="MTLWLVGMMGTGKSSAGRLAAERLGVEFVDVDEAVASEVGMAIS
                     ELWERLGEGEFRRIETAMIEQVAGTEAVVSTGGGAVLAAANRRRMRDTGRVVWLRATP
                     AVLDGRLDDAGDRPLLGGDSDRTNTITRLLEERSRAYEDAADYEIDTSPMSVEEVAMR
                     IEQLWTL"
     gene            complement(2016..>3151)
                     /gene="aroC"
                     /locus_tag="VFU96_02345"
     CDS             complement(2016..>3151)
                     /gene="aroC"
                     /locus_tag="VFU96_02345"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011905738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU5112126.1"
                     /translation="ESHGPGLVTIIEGLPRGLEVAPEQITDELARRRMGYGRGKRMAI
                     ERDEIEILGGIRHGRTLGSPVAVLIRNTEWPRWSEEMSPEPGPSKKMVTRPRPGHADL
                     AGMLKYGTDDARDILERASARETAARTVAGVLSKIFLAVVGVRVFSHVVAIGAAASEG
                     PMPGPDDQDRIDDSPVRCLDTTAEAAMIAAIEDAKEQRDTLGGVFEVLGFGVPPGIGS
                     HVHYDRRLDALLATAIMSIPAIKGVEIGDGFEVAALPGSKSHDEILLDDGVLTRPTNR
                     AGGTEGGMSNGETIRVRGAMKPISTLMRPLRTVDMTTGEPADAVRERSDVCAVPAAGV
                     VGEQMTAFILAGEFQRKFGGDTVDEFVAAADRYRSGLSDRPGR"
BASE COUNT          503 a         1099 c          985 g          564 t
ORIGIN      
        1 tgaccggctt ggtggcgccg gtcctggtgt cgcctttgac cgcgggctcg gcgtaggtga
       61 cttcgagttc cacggctgcg ggcagctcga cggcgatggg tgttcctcga tagagcgaca
      121 ggctggcagt cattccctcg accagatagc cggcagcggc ccctacgtca tcaccgctga
      181 gtgcgagttg cccgaagtcc tcgaggttca tgaaatggaa accgagatcg tctcggtaga
      241 ggtactgatg gtcccgccgc tcgatgatcg cctgatcgac gttctcgtcg gccctgaacg
      301 tgcggtcgag cacctggccc gtctccaggt tcttgagctt catccggacg aaggcccggc
      361 ccttgccggg cttgacgtgc tgatactcga cgatggtgaa caggccgtcg ctcaactgga
      421 gcgccatccc cggcttgacg tcgttggtgc tgatcatgat gcccgcagtg tggtccgaac
      481 ccgagagtga agataaaccg ggtcagagac cgacggcgtc gaggccgagg tcgaggtcgg
      541 ctgcttccac gtgctcgatc acggggtcag cgatagcccg gaggagaacc atccgcattc
      601 ccgaagagtc gcgcttcttg tcgtgacgga gcagatcttc gacgcgggct ctctccaggc
      661 cgtccaccct ggtggggaga ccgagcccgc tgatggcggc ggccatccgg tccaagccct
      721 ggaaccgaag cctccgctcc gatatccgac cggccgcgac catgccgatc gccaccgact
      781 cgccatgcga cagtggtgag gcgtattcga cggcgtggcc gatggtgtgc ccgaaattga
      841 ggatcgcccg cagacctgag tcccgttcgt cttctcctac caccctgacc ttcaccgcgg
      901 ccgccttggt gaccacctct tccaacgagg cctcctcacc gttctcctcg agcagctcga
      961 aaaggtccct gtcaccgacg agacccgtct tgagggcctc cgccaggccc tcccgacgaa
     1021 gggtcgtcgg gaggtcttcg agggtgttga cgtcgacgat caccctggtt gggtgccaga
     1081 agaggccgac caggttcttg ccggccagat tgacgccggt cttgcctccg atggcggcgt
     1141 caaccgcagc gagaagcgtg gtcggaacat ggacgacctc gaccccacgg agccacgtgc
     1201 cggcgacgaa cccggcgaga tcggacaccg agcccccgcc cacccccacg atcgtgtcgt
     1261 ggcggcccag cccgaaccgg gcgagagcct cgtacacgga tccggcgacg accagggtct
     1321 tggcctcttc cctgtcgggg agcccgatca cctcgatctg gaggcccgca tcctgcaact
     1381 gtcgcccgac gtccagggcg atatgggtcg cacccggctg ggtgaccacg gcgacccttt
     1441 cacggtcaga tcgcgccggc aggatcgggt cgggcaggcc gcgccccacg accagctctg
     1501 tcttgcccgc gacgatcaga gcgtccaaag ctgctcgatc ctcatggcga cttcctccac
     1561 cgacatcgga gacgtgtcga tttcgtagtc tgccgcatcc tcgtaggccc gtgatcgctc
     1621 ctcgagcaac cgagtgatcg tgttggtccg gtcggagtct ccccccagga gcggacggtc
     1681 tccggcgtca tcgagcctgc catcgagcac ggcgggcgtg gcccggagcc acacgactct
     1741 gcctgtgtcc cgcattcgtc gtcgattggc tgcagccagg accgcaccac cgccggtgga
     1801 gacgacggct tcggtgccgg cgacctgctc gatcatggcg gtctcgattc ttcgaaactc
     1861 gccttccccg agccgttccc acagttccga gatggccatc ccgacttccg aggccaccgc
     1921 ctcgtcgacg tcgacgaatt ccactccgag ccgctcagcg gccagccgcc cagcgctgga
     1981 tttcccggtg cccatcatcc cgaccaacca cagggtcatc tcccgggtcg atcggagagg
     2041 ccggaccgat atcgatcggc ggcggccacg aactcgtcga ccgtgtcccc accaaacttt
     2101 cgctggaact cccccgccag gatgaaggca gtcatctgct caccgactac ccctgcggcg
     2161 ggcaccgcgc agacgtccga ccgctctcgc accgcatctg cgggctcgcc cgtggtcatg
     2221 tcgaccgtgc gcaggggccg catcagggtc gatatcggct tcattgcccc ccgaacccgg
     2281 atggtctcgc cattgctcat cccgccctct gtgcccccgg cgcggttggt cggacgggtg
     2341 aggacgccgt cgtcgaggag gatctcgtcg tgggacttgg acccgggtag tgcggcgacc
     2401 tcgaatccgt ctccgatctc gacacccttg atggccggga tcgacatgat ggccgtggcg
     2461 agcagcgcgt cgagacgacg gtcgtagtgg acgtgcgagc cgatcccggg gggaactccg
     2521 aagcccagga cctcgaatac gccgccgaga gtgtcgcgct gctctttggc atcttcgatc
     2581 gcggcgatca tcgccgcctc agccgtggtg tccagacagc gcaccgggct gtcatcgatc
     2641 cgatcctgat cgtcggggcc gggcatcggg ccttcgctgg cggccgcacc gattgccacc
     2701 acgtgactga acacgcggac acccaccaca gccaggaaga tcttggaaag gacgccggcc
     2761 acggtccgag ctgcggtctc ccgcgccgag gcccgttcca gaatgtccct ggcatcatcg
     2821 gtcccgtact tgagcatgcc ggcgagatcg gcgtgtcctg gccggggtcg ggtgaccatc
     2881 ttctttgatg ggcctggttc cgggctcatc tcctcggacc atcgcggcca ttccgtgtta
     2941 cggatcagga ccgccaccgg cgagccgagt gtgcgaccgt gtctgatccc gccgaggatc
     3001 tcgatctcat ctcgctcgat tgccatgcgc ttgccccgtc catagcccat ccgccgtcgt
     3061 gccagctcgt cggtgatctg ctcgggcgcg acctccagcc cgcgaggaag tccctcgatg
     3121 atggtcacca agcccgggcc gtgggactca c
//
LOCUS       DASTXO010000114         7646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1259113, whole genome shotgun sequence.
ACCESSION   DASTXO010000114 DASTXO010000000
VERSION     DASTXO010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7646
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259113"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VFU96_02350"
     CDS             complement(<1..513)
                     /locus_tag="VFU96_02350"
                     /inference="COORDINATES: protein motif:HMM:NF019884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEU5112127.1"
                     /translation="MDSAERLLKLLALLEGRIDWTAEELATRLAVTPRTVRRDITRLR
                     SLGYPVEAVAGPGGGYRLGAGGKLPPLLLDDEEALAVALGLRVATATAVGGLEVASLS
                     ALSKLEHVLPPRLRTQLEDISEATVSTVTTSAFSVDHGSLAVAASAARTKQRMRFGYV
                     DTHGQVTERHT"
     gene            598..783
                     /locus_tag="VFU96_02355"
     CDS             598..783
                     /locus_tag="VFU96_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112128.1"
                     /translation="MLASAGFEMIRHLDEELRHASLRRFWWKHVPQDPEPARITEAEV
                     IELVFGPHCEAEEPLGA"
     gene            824..1366
                     /locus_tag="VFU96_02360"
     CDS             824..1366
                     /locus_tag="VFU96_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5112129.1"
                     /translation="MTSLPLKPDRFGDMELVFGDRGVARNCFCMHWRRPDGGFGDKRD
                     NRERFADVAAEGRPPGLIGYVEDNPVGWVQVGPRREFPTLDRSRLLKRVDEIEPWSIN
                     CFVVRTGYRKRGVGAGLLTAAIDFAKHEGAEVIEAYPVDGPRSSAVDYFTGTLSMFDE
                     HGFVELIRRNDSRPIVRLTV"
     gene            complement(1403..2230)
                     /locus_tag="VFU96_02365"
     CDS             complement(1403..2230)
                     /locus_tag="VFU96_02365"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5112130.1"
                     /translation="MTLLYVLWSIIPVGIAVLFSFNSGRSRSTWQGFSTRWYVGDPAA
                     AESVLYDPSLRFALNQSLKLAILTMLVATPIGVALALGLARWRGRGVKASNLLMLIPL
                     VTPEIVMGVALFLVFAEVYDFVPFGTWTQFLGHVTFSISFVVIIVRGRLFAIGRDYEE
                     AAMDLGASQFEAMRRVLLPLLAPALLASLAIVFAISIDDFVISQFLIGGAESVTIPVQ
                     LYSGARLAPPPSLNALATILMVVTIGAIVVAGLLFRALNKRSGGEGSAIESVAGLEM"
     gene            complement(2302..3246)
                     /locus_tag="VFU96_02370"
     CDS             complement(2302..3246)
                     /locus_tag="VFU96_02370"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5112131.1"
                     /translation="MPTNLASGAEYATVGQRRWVWPGFALPGVLWLILLFLVPFYAVF
                     AIAMGQLDPILQRATPEWNPFYWDLTVMGDAFAELFGGQLGVIALRTLAYVFIASLIC
                     VLVGYPVAYFVSRKAGRWRWLWLLLILAPFWINYLMRMMSWVNLLSPDGQVNRWLTTI
                     AGWFSVAFTPPDWLAGNHVVVILGLVYGYIPFFILPLFAALDRIDEATLEASRDLGAS
                     NAQTFWRVTWPLSRNGVLGGLVIIMLPMFGDYYTPNLLSGSPRTRMYGNTIDQYINQT
                     TSGGAVGAALTIILMIFIAGLMVYYLRSINRASQEARE"
     gene            complement(3266..4558)
                     /locus_tag="VFU96_02375"
     CDS             complement(3266..4558)
                     /locus_tag="VFU96_02375"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU5112132.1"
                     /translation="MAYHPRENWSPQNRKRVYTRRDFLQRAALLGIALPALPSLLAAC
                     GDREGQEGAELAIGTPASPAQQPLFDDNPAIDSGLEPEAGPLLLYNWEDYMNPETIPL
                     AAEALGVEIQVTTFVNEEEALARLSSGEVQFDVWFPTSQRVPVAVAASLIQPLNHDYL
                     PNLDANVWPQLVDPYYDQGSRYTVPYVTYTTGIGWRTDLADSADIEGQANPWDVFWNE
                     KYSGVIGMLDSFTDVIPVALFHNGVSDPSQATEAELIAAGDALVELIDLVDIRYRIDG
                     SYAGLPEGRYAIHQAWSGDLVNSQYFWPGGVDEGTTRYLWPAKSEGSTTKGAISSDTM
                     TVLKGSERPVLAHAFLNYMLDEANALKNFEYVGYQPPQNGLTSEYLISEEWLLPHLAP
                     ALVAPEDFELDSAWVQGPLDAATEALWVEQWNRANSGG"
     gene            complement(4613..5224)
                     /locus_tag="VFU96_02380"
     CDS             complement(4613..5224)
                     /locus_tag="VFU96_02380"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU5112133.1"
                     /translation="MSPRPSLTATRRNEILAATSATIADRGLCETRIADIADHIGASP
                     ALILYYFPSKAALVTEALVYQDQMFHEAVKGELEVATTAAAKLAILIEASCPKPSRGN
                     GDLDGWVLWPAAWEMSRHDPTLAAARARLDESWRAQIAAIVEEGIAAGEFTDVDASDF
                     SIQLAALLDGLAIEVMLRDPTVDATRMRKLAFDFVEMALNSRV"
     gene            5314..7350
                     /locus_tag="VFU96_02385"
     CDS             5314..7350
                     /locus_tag="VFU96_02385"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEU5112134.1"
                     /translation="MSLDPMLKARSVAVVGASERPGSVGDQTIRQLISGGFDGDVHVV
                     NPRYETVHGLDAVASLDAIGKSVDLAVLAVANGQLEAEMEKALDVARSVAIFASCHGE
                     ASGGGSLRDRLRDLAESGGIPICGGNGMGFLNVEERLRVCGFYQPEDLRPGSVTFLSH
                     SGSLFSAMLHNRRAIRFNLVVSTGLEINTTMDDYMEWALGLESTRVVALFLETIRDPE
                     GFRHALRRAEEGDVPVVALKVGSTRKGREAVATHSEGIAGDDAVYEALFEAHGVHRVW
                     SMDEMADTIELFASNRRATAPGLAAVHDSGGERALLIDTAERVGVPLPHLGEAATTRL
                     AQVLDPGLEPVNPVDAWGTGRDAEDVFVECLEAVADDPAVGAVAFCVDLTAEEKPDYA
                     YSDAAFTAADRTGKPLLVLSNLTTTVDAAQAARLREGGIPVLEGTETGLRAIRHLLDR
                     HTRSIRPNSAPRLSSPSTPTPTIAGETAALHVLSTYGIPVPRFEAVDDEAQLAEVARS
                     IGYPLVLKTAEPIDHKTEVRGVLVGIANEESLRAGYQDLTARLGPRVIVAEMIREGVE
                     VGLGMVHDEQFGPVVIISAGGRLIETLADRVAVLPPIDKERAIRMLGRLRIHTVLDGV
                     RGGPPADLDSLAEVIARFSELAADGAGTIGAIDVNPVIAGPGGAVAVDALMKPR"
     gene            7347..>7646
                     /locus_tag="VFU96_02390"
     CDS             7347..>7646
                     /locus_tag="VFU96_02390"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEU5112135.1"
                     /translation="MSGSNLLIEDTGPIRRLTLNRPEKLNALDAALVEALSEALLEAA
                     DLTDIRVIVIAGAGRSFCAGYDLTEEPAAGSNLDNLTHSLDRLLEVFDHPRPIIAQ"
BASE COUNT         1411 a         2375 c         2517 g         1343 t
ORIGIN      
        1 ggtgtgtctc tccgtcacct ggccgtgtgt gtcgacgtac ccaaagcgca tcctctgctt
       61 cgtccgtgcc gccgaggctg ccacagcaag ggacccatgg tcgacggaga acgcggaggt
      121 ggtcacggtg gacaccgtcg cttcggagat gtcctccagc tgagtgcgga gtctcggggg
      181 caggacgtgc tcgagcttcg acagggcaga cagagaggcg acttccagac cgccgaccgc
      241 cgtcgccgtc gccactctca gccccaaagc aaccgccagc gcttcctcgt cgtcgaggag
      301 caagggagga agcttgcctc cggctccgag tcggtacccg ccaccggggc cggccacggc
      361 ctccaccggg tacccgaggg agcgcagacg ggtgatgtca cgtcgcaccg tcctcggggt
      421 cacagcgagg cgggtcgcca gctcttcggc ggtccagtcg attcgtccct cgagcagggc
      481 gaggagcttg agcagacgtt cggcgctgtc catgatcctc gattctctgg ggttatgcgg
      541 acagatcctg tccgcatcat gtcctacggt gacggtagtc gcaggaagga agatctcatg
      601 ctcgccagtg ccggtttcga gatgatccgc catctcgacg aggagttgcg tcatgcgtcg
      661 ttgcggaggt tctggtggaa gcacgtcccc caggaccccg agccggcgcg catcacagag
      721 gcggaggtga tcgagctggt cttcggccct cattgcgagg ctgaggagcc gcttggtgcc
      781 tagggagccc tggtcacatt cgggcgagac cgatctcggc ccgatgacca gtcttcctct
      841 caagccggac agattcggcg atatggagct ggtatttggc gatcgtggag tcgccaggaa
      901 ctgcttctgc atgcactggc gcagacccga cggcggcttc ggggacaagc gagacaaccg
      961 cgagcgtttc gccgacgtgg cagccgaagg acgaccaccc ggcttgattg ggtacgtcga
     1021 ggacaatccg gtcgggtggg tccaggtcgg accgcgtcgc gagttcccaa cgctcgaccg
     1081 atctcgtctg ctcaagcggg tcgacgaaat agagccatgg tcgatcaatt gcttcgtcgt
     1141 gcgaaccggc taccggaagc ggggcgtcgg agccgggctc ctgaccgccg cgatcgattt
     1201 cgccaagcac gagggagctg aagtgatcga ggcctatccg gtcgatggcc ctcgcagctc
     1261 tgcagtggat tacttcaccg ggacgctgag catgttcgac gagcacggct tcgtcgagtt
     1321 gatccgccgc aacgactcac gaccgatcgt tcgcctcacg gtgtgagtcg ccgcgtcttt
     1381 gcgtcctctc gccgaggcgc tctcacatct cgagcccggc caccgactct atggccgatc
     1441 cctctccacc gctccgcttg ttcaaggccc tgaacaagag acccgcgacc acgatcgcac
     1501 cgatggtcac caccatcagg atggtggcca gggcattgag ggaaggtggc ggagcgagcc
     1561 gcgcccccga gtagagctgg accgggatgg tgacggactc cgcgcccccg atcaggaatt
     1621 ggctgatgac aaaatcgtcg atcgagatgg cgaagacgat ggcgaggctg gcgaggagcg
     1681 ccggggcgag aagggggagc agcacccggc gcatcgcctc gaattgggaa gccccaagat
     1741 ccatggcggc ttcctcgtag tccctgccaa tggcaaacag acgcccccga acgatgatca
     1801 ccacgaaaga gatcgagaat gtgacgtgtc ccaggaactg ggtccaggtt ccgaagggaa
     1861 caaagtcgta tacctcggcg aagaccagaa agagggcgac ccccatcacg atctctgggg
     1921 tcaccagtgg gatcagcatc aacagattgg acgccttgac ccctcttcct ctccacctcg
     1981 ccagaccgag tgcaagggcg actcctatgg gagtggcgac aagcatggtc agaatcgcca
     2041 gcttgaggct ttggttgagc gcgaaccgca gggaagggtc gtacagcacc gactcggccg
     2101 ccgccggatc accgacgtac cagcgagtgg agaacccctg ccaggtgctt ctcgatctgc
     2161 cggaattgaa cgagaacaga acagcgatcc ctaccgggat gatggaccag aggacgtaga
     2221 gcagggtcac cagaggcagc atccgcgatt tgccccaggg gttggtaatc gcagaccgga
     2281 cccgcgacgg gctggccgac gtcactctcg cgcctcctgc gaggcgcgat tgatcgaccg
     2341 caggtaatac accatcagtc cggcgatgaa gatcatcagg atgatggtca gtgcggcccc
     2401 gaccgcgccc ccactggtgg tctgattgat gtattggtcg attgtgttcc cgtacatgcg
     2461 ggtgcggggc gatcccgaca agaggttggg cgtgtagtag tccccgaaca tggggagcat
     2521 gatgatgacc aggccgccca gtactccgtt tcgagacagt ggccaggtca cgcgccagaa
     2581 ggtctgcgcg ttgctggccc cgaggtcacg cgaggcttcc aaggtggctt catcgatccg
     2641 gtccagcgcg gcaaataggg ggaggatgaa gaacgggatg tacccgtaga ccaatcccag
     2701 gatgaccacc acatggttgc ctgccagcca atcgggtggg gtgaatgcga ctgagaacca
     2761 acctgcgatc gtggtcaacc agcgattgac ctggccgtcc gggctgagca ggttgaccca
     2821 ggacatcatc cgcatcaggt agttgatcca gaaaggcgcc aggatcaaca gcagccacag
     2881 ccaacgccaa cgccccgcct ttctcgacac gaagtaggcc accgggtatc cgacgaggac
     2941 acagatgagc gacgcgatga agacgtaggc cagggttcga agggcgatca cgccgagctg
     3001 accaccgaag agctcggcga acgcgtcgcc catgacggtc aggtcccagt agaacgggtt
     3061 ccactccggt gtggcccgct gcaggatcgg gtcgagttgg cccatcgcga tcgcaaacac
     3121 cgcgtagaac gggaccagga agagaagaat cagccaaagg acaccgggca gggcgaagcc
     3181 gggccacacc catcgtctct gcccgaccgt cgcgtactcg gccccggagg cgagattggt
     3241 gggcattgcc ctctaagccg ccgtgtcaac cacccgagtt ggcccgattc cattgctcga
     3301 cccacaacgc ctcggtggct gcgtcgagcg ggccctggac ccaggccgag tccaactcga
     3361 aatcttcagg tgcgaccagg gccggtgcca ggtggggaag aagccattcc tcgctgatca
     3421 ggtactcgga agtgagaccg ttctgcggag gctggtagcc gacgtattcg aagttcttga
     3481 gggcgttggc ctcgtcgagc atgtaattga ggaacgcatg cgccaacaca gggcgctctg
     3541 agcccttgag caccgtcatc gtgtcactcg atatcgcccc cttggtggtc gacccctcgc
     3601 tcttggccgg ccacaggtat cgcgtggtgc cctcatccac cccaccaggc cagaagtact
     3661 gggagttgac cagatcgccc gaccatgctt ggtggattgc gtatctcccc tcgggaaggc
     3721 cggcgtaact gccatcgatc cggtatcgga tgtcgacgag atcgatcaac tcgaccaggg
     3781 cgtcccctgc ggcgatcaat tcggcctcgg tggcttgcga cgggtcggac accccattgt
     3841 ggaaaagggc gactggtatc acatcggtga acgaatcgag catgccgatc acccccgagt
     3901 acttctcatt ccaaaagaca tcccagggat ttgcctggcc ctcgatatcg gcgctgtcgg
     3961 cgaggtcggt tcgccacccg atgcccgtgg tgtacgtcac gtaaggaacc gtgtaacggg
     4021 atccctggtc gtaataggga tcgaccagct ggggccagac gttggcatcg aggttgggca
     4081 ggtagtcgtg attgaggggt tggatgagag atgctgctac cgccaccggg actcgctgtg
     4141 aggtcgggaa ccaaacgtcg aactgaacct caccggacga gagacgggcg agcgcctctt
     4201 cctcgttgac gaaggtggtc acctggatct cgaccccgag cgcctccgca gcgagcggga
     4261 tggtctccgg gttcatgtag tcctcccagt tgtacaacag gaggggtccg gcctcgggct
     4321 ccaacccgga gtcgatggcg gggttgtcgt cgaaaagtgg ttgctgcgcc gggctggctg
     4381 gggtgccgat ggctagttcg gcgccttcct ggccctcgcg atcgccgcag gcggcgagca
     4441 ggctgggaag ggcgggcagc gcaatgccga ggagcgccgc cctctgcagg aagtcccttc
     4501 gggtgtagac ccttttccgg ttctgagggc tccagttctc cctggggtga taagccatgt
     4561 ttcctcctcg gcgctcgggt gacactaaac cgctgttcag gtatcgcgtc ggtcaaaccc
     4621 gggaattgag tgccatctcc acgaagtcga aggccagctt tcgcatccgc gtcgcgtcca
     4681 cagtggggtc gcggagcatg acctcgatgg ccagcccatc gagcagggcg gcgagctgga
     4741 tggagaaatc ggaggcatcg acgtccgtga actcgccggc ggctatgccc tcctcgacga
     4801 tcgccgcgat ctgggccctc catgactcgt cgagccgggc gcgggcggcg gcaagggtgg
     4861 ggtcgtgtcg ggacatctcc cacgcggcag gccacagcac ccacccatcg agatccccgt
     4921 tgccacgaga tggcttgggg caactggctt ctatgaggat ggcgagcttg gcggccgcag
     4981 tcgtcgcaac ctccaactca cccttgaccg cttcgtggaa catctggtcc tggtagacca
     5041 gcgcctcggt caccagcgcc gccttcgatg ggaagtagta gaggatgagg gcggggctgg
     5101 ccccgatgtg atcggcgatg tcggcgattc gggtctcgca caacccccgg tcggcgatag
     5161 tggcgctggt ggcagccagg atctcgttgc gtcgggtcgc ggtcaggctg ggtcgagggc
     5221 tcaccggcca atcttggcac agcatcccag ttcattgcgg ccgtaccgtc gcccccggta
     5281 cgctgaatcg ccgttcagga tccgctcacg atgatgtcac tcgaccccat gctcaaggcc
     5341 cgatccgtcg ctgtggtcgg cgccagtgag cgtcccggat cagtggggga tcaaaccatc
     5401 cgacagctga tctcgggcgg tttcgacggc gacgtgcatg tggtgaatcc tcgctacgag
     5461 accgtccacg ggctcgacgc cgtcgcctcg ctggacgcca tcggcaaatc ggtcgacctg
     5521 gcagtcctgg cggtagcgaa cggtcagctc gaagccgaga tggagaaggc gctggacgtc
     5581 gctcgatctg tggcgatatt cgccagctgt cacggcgagg catctggtgg agggtcgctt
     5641 cgtgatcgac tgcgtgatct cgcggaatcg ggagggatcc cgatctgcgg cggcaacggg
     5701 atgggtttcc tcaacgtcga ggagcggctc cgggtgtgcg ggttctacca accggaagac
     5761 ctccgccccg gctctgtgac ctttctcagc cattcgggct ccttgttctc ggccatgctc
     5821 cacaaccggc gtgccatcag gttcaacctc gtcgtgtcca ccggcctcga gatcaacacg
     5881 acgatggacg actacatgga atgggcgctg ggactggagt ccacccgtgt cgttgccctg
     5941 tttctcgaga cgatccgcga cccggaggga tttcggcacg ccctcaggcg agcagaggag
     6001 ggggatgtgc ccgtcgttgc cctcaaggtg ggctccaccc gcaagggccg ggaagctgtg
     6061 gctacgcact ccgagggaat cgcaggagat gacgccgtct acgaggcgct cttcgaggcg
     6121 cacggcgtcc atcgtgtctg gagcatggat gagatggccg acacgatcga gttgttcgcc
     6181 tcgaaccgca gggccacggc tcccggtctg gccgcggtcc atgactccgg cggcgagcgg
     6241 gcgctgctga tcgacacagc agaacgggtc ggagtcccgt tgccccacct gggagaagcg
     6301 gcgacgactc gtcttgcgca ggtcctcgac ccgggtctcg agccggtcaa tccggtcgac
     6361 gcctggggca ccgggcggga cgccgaagat gtcttcgtcg agtgcctaga ggcggtggcc
     6421 gacgacccgg cggtcggcgc agtcgccttt tgcgtggatc tcaccgccga ggagaagccc
     6481 gactacgcct attcggatgc tgcgttcacc gctgccgatc gcaccgggaa gccgttgttg
     6541 gtgctgagca acctcacgac gaccgtcgac gccgcccagg cggcgcgtct tcgtgagggt
     6601 ggcatcccgg tcctcgaagg gaccgagacc ggcctcaggg cgatccggca cctgctggat
     6661 cgccacacgc gatctatcag accgaattcc gcaccaaggc tgagcagccc atcgactccg
     6721 acaccgacga ttgcgggaga aaccgccgcg ctgcacgtcc tgtcgacgta cggcatcccg
     6781 gtgccgcggt tcgaagccgt cgatgatgag gctcagctgg ccgaggtcgc acgctcgatc
     6841 ggctacccgc tcgtcctcaa gacggcagag ccaatcgatc acaagaccga ggtccgtggt
     6901 gtgttggttg ggatcgccaa tgaggagagc ctccgtgccg gataccagga cctgacggca
     6961 aggctcgggc ctcgagtcat cgtggccgag atgatccgcg agggcgttga ggtcggtctg
     7021 ggaatggtcc acgatgagca attcggcccc gtggtgatca tctccgccgg cggccgactc
     7081 atcgagaccc tggcggatcg cgtggcggtg ctgccaccca tcgacaagga aagggcgatt
     7141 cgcatgttgg gccggctccg gattcatacc gtcctcgacg gcgtgcgcgg tggccctccg
     7201 gccgatttgg actcgctcgc cgaggtcatc gctcgattct cggagctggc ggcggatgga
     7261 gccgggacga tcggggctat cgacgtgaat ccggtgatcg ccggtccggg gggcgcggtc
     7321 gccgtagacg cgctgatgaa gccacgatga gcgggtcgaa tcttctcatc gaagacaccg
     7381 ggccgattcg ccgcctcacc ctgaatcggc cagaaaagct caacgcactc gacgcggcct
     7441 tggtcgaggc gctctccgaa gctttgctcg aggctgcgga tctcacggac attcgcgtga
     7501 tcgtcatcgc cggagccggg cgctcgtttt gcgccggtta cgacctcacc gaggaaccgg
     7561 cagcggggag caatctggac aacctcactc acagtcttga ccgcttgctt gaggtcttcg
     7621 accaccctcg accgatcatt gcccag
//
LOCUS       DASTXO010000115         2394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1406706, whole genome shotgun sequence.
ACCESSION   DASTXO010000115 DASTXO010000000
VERSION     DASTXO010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406706"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VFU96_02395"
     CDS             complement(<1..633)
                     /locus_tag="VFU96_02395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01726.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5112136.1"
                     /translation="MTEAEAPLREPGVSPGRARRLAFHRRQRRRSLLVGGLSTVVVFG
                     VVILVVVNSPGWPSVRESFFSWEDFKASFPDILSGFVRNVRIFLIAEVFILIVALVLA
                     VIRSSRSPLLTPLRLAATAYVDLIRGIPTIILLFLLGFGVPALRLQGVPNSQEFWAIT
                     TLVVSYSAYVAEVYRAGIDSVHESQTAAARSLGLSSAQSMRWVVLPQAVRR"
     gene            complement(643..1476)
                     /locus_tag="VFU96_02400"
     CDS             complement(643..1476)
                     /locus_tag="VFU96_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009327418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5112137.1"
                     /translation="MKKSIAFIASLALLVAACAAEEGGGGGDASCEKGDLTLVEEGVL
                     TIGTDEPSFPPWFDFEGGPDTGTGFESAVAYAVADELGFTEEEVNWVVVPFNNAFAPG
                     EKDFDFDINQVSISEERDEAVDFSDGYYDVNQALLAFSDSEIASATTIDELKAYRLGA
                     QVGTTSLDFITDVIQPESEPLVYDTNADAKAAFDAGQLDGLVLDLPTAFFVSAVEIEG
                     STVVAQFPSAGESEQFGMVFEEGNPLRDCVNQALANVDLEAIQQEWLSDVVEAPIIQT
                     S"
     gene            1613..>2394
                     /locus_tag="VFU96_02405"
     CDS             1613..>2394
                     /locus_tag="VFU96_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU5112138.1"
                     /translation="MKRRIIWLIAAMALIAAACGGGEGTATTEATDTTEATETTQATE
                     TTQVTETTAATEGDLLSAIEEEGVLTVSTDPAYPPQSSLNEETGEYEGFDIDVATAIA
                     DQIGVEVAWEAPAWETIIAGNWNDRWDLSVGSMTITPEREEVLHFSTPYYYTPAVVAV
                     GADDDSITDLTTDLDGQTIGVCGGCTYDFYLQGNLEIPGESFEFVIDDAEIVTTDTDT
                     TAIEQLVQGRVAAVMSSATTIQAAIDAGLPIKVVGDPLFYEPL"
BASE COUNT          513 a          748 c          734 g          399 t
ORIGIN      
        1 acgccgcacc gcctggggca ggaccaccca tctcatggac tgggcggagc tgagcccgag
       61 agagcgagcc gcggccgtct ggctctcgtg gacggagtcg atgcctgccc ggtacacctc
      121 cgccacatat gccgagtagg agaccacgag ggtggttatg gcccagaact cctggctgtt
      181 ggggacgcct tggagtcgca aagccgggac accgaatccg aggaggaaga gaaggatgat
      241 ggtggggatg ccccggatca gatcgacgta ggcggttgcg gcgagacgca atggggtgag
      301 aaggggcgat cggctactgc ggatcactgc caacacgaga gccacgatga ggatgaagac
      361 ctcggcgatc aggaaaatcc gaacgttgcg gacaaacccg ctgaggatgt ccgggaacga
      421 cgccttgaaa tcctcccagc tgaagaacga ctcgcggact gacggccatc ccggggagtt
      481 gaccactacc aggatcacga ccccgaacac gacgaccgtg ctcaatccac cgactagcaa
      541 cgagcgtcgg cgctgtcgcc tgtgaaaggc caggcggcgt gcgcggcccg gcgagacgcc
      601 cggttcgcgg agcggcgcct cggcctcggt cattgggacg tgctagctgg tctggatgat
      661 cggggcttcg accacatccg agagccattc ctgctgaatg gcttcgaggt cgacgttggc
      721 cagggcctga ttgacgcaat cccgcagcgg gttcccctcc tcgaagacca tcccgaattg
      781 ctcactctcc ccagcggaag ggaactgggc gaccaccgtc gaaccctcga tctcaaccgc
      841 cgacacgaag aacgcagtcg gcagatcgag cacgaggcca tcgagctgac cggcgtcgaa
      901 tgccgctttg gcatcggcgt tggtgtcgta taccagcggc tcgctctcgg gctggatcac
      961 gtctgtgatg aagtcgaggc tggtggtgcc gacctgagca cccagccggt aggccttcaa
     1021 ctcatcgatc gtggtggccg aggcgatttc ggaatcggag aaggcgagca gcgcctggtt
     1081 cacgtcgtaa tacccatcgg agaaatccac cgcctcgtct cgctcttcgc tgatcgagac
     1141 ctggttgatg tcgaaatcga agtctttttc ccccggggcg aatgcgttgt tgaagggcac
     1201 caccacccag ttcacctcct cctcggtgaa gccgagctca tcagccaccg cgtaagcgac
     1261 ggcactctcg aacccggtgc cggtgtcggg gccaccctcg aaatcgaacc atggtgggaa
     1321 agacggctca tcggtgccaa tcgtcagcac accttcttcc acaagggtca gatccccctt
     1381 ctcgcacgag gcatcgccgc cacctccacc ttcctccgct gcacacgcgg ccacgagcag
     1441 ggcgagggat gcgatgaagg cgatgctttt cttcatgttg gagtcctccg aaatctcgtg
     1501 gaatccggga gcgtagtgct cgccgtcatc tgccccgcca aagggcgaaa catgaactct
     1561 tcgatacaat cgctcagtcc tagagggaca acgctcagga ggaacaagct caatgaaacg
     1621 acgaatcatc tggctcatcg cggccatggc gctcatcgcc gcagcctgtg gcgggggcga
     1681 gggcaccgca accacagaag cgactgacac cacagaagcg accgagacca cacaagcaac
     1741 cgaaacaaca caagtcaccg aaacaacagc agccaccgaa ggggatctcc tgtccgccat
     1801 cgaagaggaa ggcgtcctga cggtttcgac cgatcccgct tatccacctc agtcgtctct
     1861 gaacgaagag accggcgaat acgagggctt cgacatcgac gtggccacgg cgattgccga
     1921 ccaaatcggg gtcgaggtgg cgtgggaggc cccggcctgg gagacgatca tcgccggcaa
     1981 ttggaacgat cgttgggacc tcagcgtcgg ttcgatgacc atcaccccgg agcgagaaga
     2041 ggtgctccac ttcagcacgc cctactacta cacacccgca gtggtcgcgg tgggggctga
     2101 cgacgactcc atcaccgatc tgaccaccga cctcgatggg cagaccatcg gagtttgtgg
     2161 cggctgcaca tacgacttct atttgcaggg gaacctcgag atcccagggg agagtttcga
     2221 gttcgtcatc gacgacgccg agatcgtgac caccgacacc gacaccacgg ctatcgagca
     2281 actggtgcag ggtcgggtgg ccgcggtgat gtcgtccgcg acaacgattc aggctgcgat
     2341 cgatgccggc cttccgatca aagtcgttgg cgacccgctc ttttacgagc ccct
//
LOCUS       DASTXO010000116         4062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_522614, whole genome shotgun sequence.
ACCESSION   DASTXO010000116 DASTXO010000000
VERSION     DASTXO010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522614"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>4062
                     /gene="sprA"
                     /locus_tag="VFU96_02410"
     CDS             <1..>4062
                     /gene="sprA"
                     /locus_tag="VFU96_02410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HEU5112139.1"
                     /translation="QDIDENSIELYVDDAVNTSQSNIVRGRAFVDPNNRCIDPVTCLD
                     GPDADSDPDTTRSVRGTFRKLTLANQDYEILSSFYGPSYKVIRLRSPVTDQKHCLAVT
                     YRYKESVNNQPVGDSVHVGGLYADDIDLSKGTRVPALTMKLLRAPLVETPPDTTTGLF
                     STRSPFYATRELELKNIYHLAGQNIDAKSFMLAIRQGNEQPPVTTVTIGSPPALESVP
                     FIEILGLDNFDETSGFPGYDKRGHDNFVDATGPTNFVRPAIDFVRGTLFFPDPRPFAP
                     RLAADTTGRRFDRFVDSLLTRRERLTGGISAANEANPDIYDKHTVLNNDAYYYLEPIF
                     VAQTAGNEINLGRSNIIEGSDVVRINGQPIQRDKDYRIDYDLGRVTLIRQLLPADQLD
                     IDYSYAPLFQQAGKTLLGSAFRLEGRDKSLGGAFLYESRGATDLRPRLGEEPSQVVIG
                     DLNTDWKFKPSWMTRLADVLPGVRTSQPSELNIQAEMGVSLPNPNTRNEVFIDDMEGS
                     RDAVSLSMGPERWRWSSVPLRTPGQTNTIDDPGLPNPQRNAEIHWYSPLNVVKERDLK
                     PSLTQAQGAQNTHQSLAISIPRRPRIVDQNGNPTGLPDTTHKLWVGLTYPLDPGGIDL
                     SRSQFIELWVNDFRDHHNNNPTPRVRGFNVKLHIDLGVVPEDQQRAPNIIPNGALDTE
                     DRAKDGQLTVTEGNNEDTGLDSLLSVDEVPQPIPDLVTVWAEDPSGDDFELPRTDRTD
                     WDPRKWVSTNGTEENKNVEPVPDTEDMNQNNRLDGLSDYFEYTIDLGDTLSPYLATDV
                     YESYDGYAGTTDPMPLDNGWRRYRIPIDDKARQRFGNPVLETARHVRVWLEDIVLTDP
                     AEFVNGEGKLDGRPFVVLGGIEIVGNRWLATDLDSTSKAAGTTLTLNTVNTTDNAEIY
                     VPPFDPGSTRSGSQELVRREQTMAIEFTNLADRDTLEVFKTVSLDEDYSRYGKLNWYA
                     AAYQIRDYVETSDTLDYFLRFASDERGDHYYEYRARLPRSSEPLKINWKEVSLALTEL
                     SNIKLNPDFPSGGPVLYYAPGRVPGEVFVIKGRPAFTRLRRISFGLINLSGKAFPSGQ
                     LWFNELRATDVAKDRGQAQRLSVNGRVANLVNYNFMYNKADAHFQAVGQNRGTGNTDN
                     TISLQTGVDLHRFIESSRILLPFNYTYNRFMSSPRFTAGDDVVRTGALAEASDSRVMT
                     RTMSLSYSRTWGDRSNPLLRYTLGGFTANINRSQNELRNPTTVSANTQTAAAVNYSIA
                     PRQILTLGVPLTRLKIYPFPERFYWNYSFSTNEGASYNRERDSLGTLVLRNVTNARVA
                     GIDFGMDSRPIDMFHHSFRARRNLTLPTELLERV"
BASE COUNT          808 a         1447 c         1183 g          624 t
ORIGIN      
        1 caggacatcg acgagaacag catcgagctg tacgtcgacg acgcagtaaa caccagccag
       61 tcgaacatcg tgcgcggtcg cgcgttcgtg gaccccaaca accggtgcat agaccctgtc
      121 acgtgcctgg acgggcccga cgccgacagc gatcccgaca ccacccgatc ggtgcgcggc
      181 acctttcgaa agctcacgct cgccaaccag gactacgaga tcctctccag cttctacggt
      241 ccctcgtaca aggtgatccg gcttcgttcg cccgtgaccg accagaagca ttgcctggcg
      301 gtcacctatc gctacaagga gtcggtcaac aaccagccgg tcggagattc agtccacgtg
      361 ggggggctgt acgcggacga catcgacctc tcgaagggga cccgggtgcc ggccctgacc
      421 atgaagctgc tgcgcgctcc gctcgtggag acacccccgg atacgacgac cggactcttc
      481 agcacccgct ctcccttcta cgcgacgcgc gagctggagc tgaagaacat ctatcacctg
      541 gcagggcaga acatcgatgc caagtcgttc atgctggcga tccgtcaggg caacgagcag
      601 cctccggtga cgaccgtgac catcggcagc cctcccgcgc tggagtcggt gccgttcatc
      661 gagatcctgg ggctcgacaa cttcgacgag accagcggct tccccggcta cgacaagcgc
      721 gggcacgaca acttcgtgga cgccaccgga cccaccaact ttgtgcgtcc ggccatcgac
      781 ttcgtgcgtg gcactctctt cttccccgac ccgcgcccgt tcgcgccgcg cctggcggcg
      841 gacaccaccg gccgccgctt cgaccgcttc gtcgactcgc tgctcacccg gcgcgagcgg
      901 ctgaccggcg gaatcagcgc cgccaacgag gccaaccccg acatctacga caagcacacc
      961 gtgctgaaca acgacgccta ctactacctg gagccaatct tcgtggcgca gaccgccggc
     1021 aacgagatca acctggggcg cagcaacatc atcgagggct ccgacgtagt gcggatcaac
     1081 ggccagccga tccagcgcga caaggactac cggatcgact acgacctggg gagggtgacc
     1141 ctgattcgcc agctcctccc cgcggatcag ctcgatatcg actattccta cgcacccctg
     1201 ttccagcagg cggggaagac gctgctcggc agcgcgttcc gcctcgaggg ccgcgacaag
     1261 agcctgggcg gcgcgttcct gtacgagagc cgcggcgcca ccgacctgag gccccgcctg
     1321 ggggaggagc cctcccaggt cgtgatcggc gacctcaaca ccgactggaa gttcaagccc
     1381 tcctggatga cgcgcctcgc cgacgtgctg cccggtgttc gcacctcgca accctcggag
     1441 ctcaacatcc aggcggagat gggagtctcg ctccccaatc cgaacacccg gaacgaagtt
     1501 ttcatcgacg acatggaagg ctcccgcgac gcggtatcgc tgtcgatggg ccccgagcgc
     1561 tggcgctggt cgagcgttcc gttgcggacc ccgggccaga ccaacaccat cgacgatccc
     1621 ggccttccca acccgcagcg caacgccgag atccactggt actcgcccct gaacgtggtc
     1681 aaggagcggg atctcaagcc ctcgctcacc caggcgcagg gggcgcagaa cacccatcaa
     1741 tccctggcga tctcgatccc acgccggcca cggatcgtcg accagaacgg caatcccacc
     1801 gggctgcccg acaccaccca caagctgtgg gtggggctca cctatccgct cgacccgggg
     1861 ggcatcgatc tgtcgcggtc ccagttcatc gagctgtggg tgaacgattt ccgtgatcac
     1921 cacaacaaca accccacccc gcgagtgcgc gggttcaacg tcaagcttca catcgacctg
     1981 ggggtggtgc ccgaggacca gcagcgggcc cccaacatca tccccaacgg ggcactcgac
     2041 accgaggatc gggcgaaaga cggccagctc accgtcacgg aggggaacaa cgaggacact
     2101 ggtctcgaca gtctgctcag cgtggacgag gtgccccagc cgattcccga cctggtcacg
     2161 gtatgggctg aagacccgtc cggggacgat ttcgagctgc ccaggacgga tcggacggac
     2221 tgggacccac gcaagtgggt gtccaccaat gggacggagg aaaacaagaa cgtcgagccg
     2281 gtgccggaca ccgaggacat gaaccagaac aaccgactcg acgggctctc ggactacttc
     2341 gagtacacca tcgacctcgg cgacacccta tccccctacc tggccaccga tgtgtacgag
     2401 tcgtacgacg gctacgccgg caccaccgat cccatgccgc tcgacaacgg ctggcgccgt
     2461 taccgcattc ccatcgacga caaggcgcgc cagaggttcg ggaatccggt cctggagacg
     2521 gcgcgccacg tgcgggtgtg gctggaggac atcgtcctga ccgaccctgc cgagttcgtg
     2581 aacggcgagg gaaaactcga cgggcgaccg ttcgtcgtgc tgggcgggat cgagatcgtg
     2641 ggcaaccggt ggctggccac cgatctcgac tcgacttcca aggcggccgg caccaccctg
     2701 acgctcaaca ccgtcaacac cacggacaac gcggaaatct acgtgcctcc gttcgatccc
     2761 ggcagcactc gcagcggcag ccaggagctc gtgcgccgcg agcagacgat ggcgatcgag
     2821 ttcaccaacc tggccgatcg cgacaccctc gaggtgttca agaccgtctc gctcgacgag
     2881 gactacagcc gctacggcaa gctcaactgg tacgccgccg cctaccagat tcgcgactac
     2941 gtcgagacct ccgacaccct cgactacttc ctccgcttcg cctcggacga gcgcggggac
     3001 cactactacg agtaccgcgc ccggctgccc aggtcctctg agccgctcaa gatcaattgg
     3061 aaagaggtct ccctggcgct caccgagctg tcgaacatca agctcaaccc ggactttccg
     3121 tccggggggc ccgtcctcta ctacgccccc ggccgcgttc cgggagaagt gttcgtcatc
     3181 aagggacggc ctgcgttcac ccgcctgcgg cgcatctcgt tcggtctgat caacctctcg
     3241 ggcaaagcgt tcccttccgg acagttgtgg ttcaacgagc tgcgcgccac cgatgtcgcc
     3301 aaggatcgcg gtcaggcgca gcgcttgagc gtgaacggcc gcgtggccaa cctggtcaac
     3361 tacaacttca tgtacaacaa ggccgacgcc cacttccagg cggtgggaca gaaccgcggc
     3421 acagggaaca ccgacaacac catcagtctc cagaccggcg tcgacctgca ccgcttcatc
     3481 gagagctcca ggatcctgct gccgttcaac tacacctaca atcgcttcat gagctcgcca
     3541 cggttcaccg ccggggacga cgtggtccgc accggggcgc tcgccgaggc cagcgacagc
     3601 cgggtcatga cccggaccat gtccctgagc tattcacgaa cgtggggaga ccgttccaac
     3661 ccgctcctgc gttacacgct cggcggcttc accgccaaca tcaaccggtc gcagaacgaa
     3721 ctgcgcaacc cgaccacggt gagcgcgaac acccagactg cggcggccgt gaactacagc
     3781 atcgctccgc gccagatcct gacgctgggc gtgccgctca cgcgactgaa gatctatcct
     3841 tttcccgagc gtttttactg gaactacagt ttcagcacca acgaaggggc gagctacaat
     3901 cgcgaacggg attcgctggg caccctggtc ctgcgcaacg tgaccaatgc ccgcgtcgcc
     3961 ggcatcgact tcggcatgga cagccgcccg atcgacatgt tccatcactc gttccgggct
     4021 cgccgcaatc tcacgctgcc cacggagctg ctcgaacggg tg
//
LOCUS       DASTXO010000117         5045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1296142, whole genome shotgun sequence.
ACCESSION   DASTXO010000117 DASTXO010000000
VERSION     DASTXO010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296142"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="VFU96_02415"
     CDS             complement(<1..517)
                     /locus_tag="VFU96_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112140.1"
                     /translation="MTAIVIIEGLVILLLAILVAGLLKSHAEILRQLAALGVSEEGAV
                     TVGTPQTRPKTTGFEKAPSNTLSGVGLDGTEQTLSLVHGRGNTLVAFLSSGCLSCQTF
                     WTEFQGDFELPMPDTRTVIVTKGPGSESAAKLVEMAPAKVPVIMSDETWQTFRVPMTP
                     YFLLVDGQGMVI"
     gene            complement(517..1833)
                     /locus_tag="VFU96_02420"
     CDS             complement(517..1833)
                     /locus_tag="VFU96_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEU5112141.1"
                     /translation="MSTRLVAAATRALDTRTSRRGFLRRAAMVGTALVAAPTAYILRP
                     GSAYGAVVPSQCSGGARCNDGWTDFCCNIHGVNTCPPGDVVAGWWRAEGNTFCDGRSR
                     YYMDCNSASCGGCSCGGSGTCSDSCVNCNCQCNHDNCNERKVCCTRFRYGQCNNQLPC
                     IGPITCRVVTCVPPWEWDSTCTTTSAVSQSTYSQNAAGLLPDTTPKGPYRARPAVVSG
                     TSFQLRNTLSSGGANQTFDFGLPGDYPVVGDFSNAGVRTVGMVRNTRHGVAGVGSLVW
                     FLRQVEGPGSANLVIEYGRPGDIPVVGDWNGNGVHTIGVVRGNRWLLRNSNRAGDPEI
                     DFVFGQAGDIPVVGDWNGDGRTGIGVVRGSRWILRNALSAGPPDRDFTFAGNGSPVTG
                     DWDGDGRTGVGWFNGGTWTIRNIGATTSTSFTFGGNGFPLTWGRNA"
     gene            complement(1830..2339)
                     /locus_tag="VFU96_02425"
     CDS             complement(1830..2339)
                     /locus_tag="VFU96_02425"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEU5112142.1"
                     /translation="MVAAAVAAIALGLLGLAGMAKVADPVPTGGALRAARLPSTNVVV
                     RILGLFEIAASTVGLIFGGPWVTGAVALYAGFTAFTWLAVRARMPIQSCGCFGREDTP
                     PSWLHFGFNLVSLIALGVVAVTGTAVVPPGGSTPTLIGYLVFASIGVYLSYLLLTRLP
                     QTMALARTE"
     gene            2423..3331
                     /gene="cysD"
                     /locus_tag="VFU96_02430"
     CDS             2423..3331
                     /gene="cysD"
                     /locus_tag="VFU96_02430"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020635754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEU5112143.1"
                     /translation="MSGSQSSHLRALESESIHIIREAVAESENPVLLFSGGKDSALVL
                     RLAVKAFWPARLPFPVMHVDTGHNFDEVIEFRDRVVAELEADLIVASVQEAIDQGRVV
                     EDTGPRASRNRLQTETLLNALDKHRFDVVFGGARRDEEKARAKERVFSFRDEFGQWDP
                     KSQRPELWSLYNGRHRNGEHMRVFPISNWTEMDVWQYIEGEEIELPSVYFSHRRRVFE
                     RDGMLMAESPYLGLLDGEEVFEAIVRFRTVGDMTCTGGFESTASSVGEIIEEVAVTRI
                     TERGASRADDRTADAAMEDRKREGYF"
     gene            3331..4590
                     /gene="cysN"
                     /locus_tag="VFU96_02435"
     CDS             3331..4590
                     /gene="cysN"
                     /locus_tag="VFU96_02435"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013731496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HEU5112144.1"
                     /translation="MTELVRIATTGSVDDGKSTLIGRLLYDSKQIFQDQLEAVEDASR
                     KRGSDYVDLALLTDGLRAEREQGITIDVAYRYFATPKRKFVLADTPGHIQYTRNMVTG
                     SSTANIALILVDARQGILEQTRRHSFLASLLGVPHLVLCVNKMDLVEWSQERFDAVVD
                     DFRQFATRLDIHDLRFVPVSALEGDNVVNQSVNMPWYHGPTVLELLEDIHIGSDQNMI
                     DVRFPIQYVIRPHRREYQDFRGYAGKIAGGVIKRGDEVMVLPSGFTTTIRSIETYDGP
                     LEEATPPMSVVVTLEDDIDVSRGDMLCRPHNQPHASQDLDAMVAWMSEGPPLKVGSKY
                     AIKHTTRWGKAMVRDFQYRLDVNTLHRDESSDSLSLNEIGRVRLRSTTPFFFDEYRRN
                     RETGSFVLVDESTNVTVGAGMITGEGP"
     gene            4587..>5045
                     /gene="cysC"
                     /locus_tag="VFU96_02440"
     CDS             4587..>5045
                     /gene="cysC"
                     /locus_tag="VFU96_02440"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020555338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEU5112145.1"
                     /translation="MSENVVWQRGSVTPADREKATGGKGVTVWMTGLSGSGKSTIAYS
                     AEEMFVQAGRAAYVLDGDNLRHGLNADLGFGAADRSENVRRVGEVARLLADAGLIVLV
                     PVISPFASDRKMVREAHEQASLAFIEVFVDAPLEVCEQRDPKGLYARARAG"
BASE COUNT          959 a         1594 c         1627 g          865 t
ORIGIN      
        1 cgatgaccat tccctggccg tcgacgagga ggaaatatgg cgtcatcggc acgcggaagg
       61 tctgccacgt ctcgtccgac atgatcacgg gaaccttggc tggtgccatc tcgacgagct
      121 tggctgccga ttcggaacca gggcccttgg tgacgatgac cgtccgcgtg tcgggcatcg
      181 gcagctcgaa gtcgccctgg aactcggtcc aaaaggtctg acagctcaga cacccggagg
      241 agagaaacgc cacgagcgtg tttccccggc catgaaccag gctcagagtc tgctcggtgc
      301 catcgaggcc cacgccgctg agcgtgttgg atggggcctt ctcgaaacct gtcgtcttcg
      361 gcctggtctg gggtgtcccc acggtgacgg ccccctcctc ggagacgccc agagcggcga
      421 gttggcgaag gatctcggca tggctcttga gcaggccggc gacgaggatg gcgaggagga
      481 ggatgaccag gccctcgatg atgacaatgg ccgtcatcag gcgttccgtc cccaagtgag
      541 cggaaagcca ttcccgccga acgtgaagct cgtcgatgtg gtcgccccga tgttgcggat
      601 cgtccaggtg ccgccgttga accagccgac tccggtgcga ccgtcaccgt cccagtctcc
      661 ggtcaccgga gatccgttcc cggcgaatgt gaagtcacgg tcgggcgggc cggccgagag
      721 tgcattgcgg aggatccatc ggctgccacg gaccactccg atgccggtgc ggccatcacc
      781 gttccagtct cccacgacgg ggatgtctcc tgcctggccg aacacgaagt cgatctcggg
      841 gtcacccgcc cgattcgagt tgcgaaggag ccacctgttg ccgcgaacga caccgatcgt
      901 atggacgccg ttgccgttcc agtcccccac aaccgggatg tcacctggtc tcccgtattc
      961 gatgacgaga ttggcgctgc ccgggccctc cacctggcgg aggaaccaca ccaggctgcc
     1021 caccccggct acgccatgac gggtgttgcg gaccatgccg acagtcctca ccccggcatt
     1081 ggagaaatcg cccaccaccg ggtagtcgcc gggcaagccg aaatcgaacg tctggttggc
     1141 tcctccgctg ctcaaggtgt tgcgaagctg gaaactggtg ccgctgacca ccgccggccg
     1201 agcgcgataa gggcctttgg gggtggtgtc gggaagcagc cctgcggcat tctggctgta
     1261 ggtcgactgg gaaacggcgc tcgtcgtggt gcaggtcgag tcccattccc atgggggaac
     1321 acaggtgacg acccggcagg tgatcggccc gatgcaaggg agctggttgt tgcactgacc
     1381 ataacggaac cgggtgcagc agaccttgcg ctcattgcag ttgtcgtggt tgcactggca
     1441 gttgcagttc acacaggagt cggagcacgt gccggagcca ccgcagctgc acccaccgca
     1501 ggaggcggag ttgcagtcca tgtagtagcg gctccgaccg tcacagaagg tgttcccttc
     1561 cgcccgccac catccggcga cgacgtctcc gggaggacag gtgttgacac catggatgtt
     1621 gcagcagaag tcggtccagc cgtcgttaca tctcgcgccc ccgctgcact ggctcggcac
     1681 cacagcgcca tatgcgcttc ccggccgcag gatgtaagcg gtcggcgcgg cgaccaaggc
     1741 ggtacccacc atggcagcac gccgcaagaa gccccgccgc gaggttcgcg tgtcgagcgc
     1801 ccgggtcgcc gctgcaacga gtcgggtgct cattcagtcc gggccagggc catcgtttgg
     1861 gggagacggg tcagaagcaa gtaggagaga tagacgccga tggaggcaaa gaccaggtag
     1921 ccgatgagcg taggggtcga gccacctggc ggaacgacag ccgtcccggt gaccgcaacg
     1981 acacctagag cgatcagact gacgagattg aatccgaagt ggagccagga cggcggggtg
     2041 tcctcccgtc caaagcagcc gcagctctgg atcggcatcc gagcacgcac ggccagccac
     2101 gtgaaggcgg tgaacccggc atacaaggcc acggcaccgg tcacccatgg tccaccgaag
     2161 atgagaccga ctgtcgatgc cgcgatctcg aaaagcccga gaatcctcac cacgacgttg
     2221 gtcgagggga ggcgagcagc ccgcagagcg ccaccggttg ggaccgggtc cgcaaccttc
     2281 gccatcccgg ccagtccgag gaggccgaga gctatggcgg cgacggctgc ggcgaccacg
     2341 gagcgagagt agtgccgatg tctggatcac cggaatcccg acgaaatatg tctaccgggc
     2401 cagggagctt taaccttttc ccgtgtccgg atcgcaatcg agccatctgc gcgccctcga
     2461 atcggagtcg attcacatca tccgtgaagc ggtcgccgaa tcggagaacc cggtcttgct
     2521 cttctccgga ggcaaggact cggcattggt gctgcgactc gccgtcaagg cgttctggcc
     2581 ggcgcggctt cccttcccgg tcatgcatgt cgacaccggg cacaacttcg atgaagtgat
     2641 cgagttcagg gaccgggtgg tcgcggagct cgaggccgac ctcatcgtcg cctccgtcca
     2701 ggaggccatc gaccagggca gggtggtcga ggacaccggg ccccgggcct cccggaatcg
     2761 tcttcagacc gagactctgc tcaatgccct cgacaagcac cggttcgacg tcgtgttcgg
     2821 aggggcgcgt cgggacgagg agaaagcccg ggctaaggag cgggtgttct cgtttcgaga
     2881 cgagttcggg cagtgggacc cgaagagcca gagacccgag ctctggtccc tctacaacgg
     2941 ccggcatcgc aatggcgagc acatgcgtgt cttcccgatc tcgaattgga ccgagatgga
     3001 cgtctggcag tacatcgagg gtgaggaaat cgagttgcct tcggtctact tctcgcatcg
     3061 acggcgggtc ttcgagcggg acgggatgct catggccgag tcgccctatc tcggccttct
     3121 cgacggtgaa gaggtgttcg aggccatcgt gcggttccgg accgtggggg acatgacttg
     3181 caccggtggc ttcgaatcca ccgcgtcctc agtcggggag atcatcgagg aggtagccgt
     3241 cacccgtatc accgagcgcg gggccagccg tgccgacgac cgaaccgccg atgccgccat
     3301 ggaggaccgc aagcgggagg gctatttctg atgaccgagc tggtgcggat cgcaaccacc
     3361 gggtcagtcg acgacggaaa gagcacgctc atcggccggc tcctctacga ctccaaacag
     3421 atctttcagg accagctcga ggcggtcgaa gacgccagcc gcaagcgcgg ctcggactat
     3481 gtcgacctcg ccctgctcac cgatggcctt cgcgccgaac gggaacaagg catcacaatc
     3541 gacgtcgctt accgctattt cgccacacca aagcgcaaat tcgtgctcgc cgacaccccg
     3601 ggtcacatcc aatacacccg caacatggtc accgggtcat ccacggccaa catcgctctg
     3661 atcctggtcg atgccaggca gggcattctc gagcagacca ggcgtcactc ttttctcgct
     3721 tcccttctcg gcgttcccca cctcgtgctg tgtgtcaaca agatggacct cgtggagtgg
     3781 tctcaggagc ggttcgacgc cgtggtcgac gacttccgtc aattcgccac ccggctcgac
     3841 atccacgacc tccgcttcgt tccggtgtcg gcgctcgagg gtgacaatgt cgtcaatcag
     3901 tctgtgaaca tgccctggta tcacggacca acggtcctcg agttgctcga agacatccac
     3961 atcggcagcg accagaacat gatcgacgtg agattcccta tccaatacgt gatccgcccc
     4021 catcggcgcg agtaccagga cttcaggggc tacgcaggca agatcgcagg tggggtgatc
     4081 aagcggggcg acgaggtgat ggtcctcccg agcggtttca ccaccacgat ccgttcgatc
     4141 gagacctacg acgggccctt ggaggaggca accccaccga tgtcagtggt cgtgactctg
     4201 gaggacgaca tcgacgtgtc acgcggcgac atgctttgcc ggccccacaa ccagccgcat
     4261 gcatcgcagg acctcgatgc gatggtggca tggatgtccg aaggcccacc gctgaaggtg
     4321 ggctccaagt acgccatcaa gcacaccacc cggtggggga aggccatggt tcgtgacttc
     4381 caatatcgac tcgacgtcaa cacgcttcat cgggacgaat cctccgattc gctcagcctc
     4441 aacgagatcg ggagggtgcg acttcgatcg acgacgccgt tcttcttcga cgagtaccga
     4501 cgcaatcgag aaaccggctc attcgtcctc gtcgacgagt cgaccaacgt cacggtcgga
     4561 gccgggatga tcaccggcga gggcccgtga gcgagaacgt ggtttggcag cgcgggtcgg
     4621 tgacgccagc ggaccgggag aaggcaaccg gcgggaaggg tgtcaccgtg tggatgaccg
     4681 gcctctccgg cagcggcaag tcgaccatcg cctactcggc cgaggagatg tttgtccaag
     4741 ctggccgagc cgcctacgtc ctcgatgggg acaacctgcg ccacggcctc aatgccgatc
     4801 ttgggttcgg ggcggccgac cgtagcgaga acgtgagacg agtcggtgag gtggctcggc
     4861 tcttggccga tgccggcttg atcgtgctgg tcccggtgat ctcaccgttc gcctcggacc
     4921 gcaagatggt gcgggaggct cacgagcagg cgagcctggc cttcatcgag gtgttcgtcg
     4981 atgctcctct agaggtatgc gagcagcgag atcccaaggg cctctacgct cgtgctcggg
     5041 ccggc
//
LOCUS       DASTXO010000118         3267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1811996, whole genome shotgun sequence.
ACCESSION   DASTXO010000118 DASTXO010000000
VERSION     DASTXO010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1811996"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VFU96_02445"
     CDS             <1..498
                     /locus_tag="VFU96_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEU5112146.1"
                     /translation="LIADEPTTALDVTIQARILNLIAELQQRLGTSVLYISHDLSLVR
                     EISDRVAVMYAGRIVEIGRTEDLFREPLHPYTRSLMSAIPSSTSTRGRLAAIEGNVPE
                     LIDPQPSCRFHSRCPHALEICRNFDPRFLRHDVDHGVACHGHDDPADLGFPTSEVPTV
                     GSMSR"
     gene            495..1667
                     /locus_tag="VFU96_02450"
     CDS             495..1667
                     /locus_tag="VFU96_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEU5112147.1"
                     /translation="MTDVRVGAITTEPYPRFSDGELQARRQAMLEVMDGAGVAHLVAY
                     GSERTGSAVQWLSEWPVTREAALVLSPGEPIRLLVQHYNHLPNARRIASDCDVEWGGP
                     STINRLGELLRARLTDGQRVGVVGPLRFQDHRDLAVSVGDLVSLDRQYQRLRLVKSAE
                     EIERLEVAAHLSDRAVEAIAAEARVGMDEHRLAAIIESAYLAEGGTNHIHYFAATPMA
                     DPSICVPSQFHSTRRLQAGDILFCEISAAYWGYAGQVLRSFAIGADPTPLYNDLHRAA
                     DGAFDAIAAVTRPGTHVRDLVDASSVIEEMGFTIYDDLVHGYGGGYLPPVLGSRSRPN
                     LPTPDMVLEEGMALVIQPNVITTDERAGVQTGHLVVVTGDGHRPLHDVPRGLRRIG"
     gene            complement(1686..2831)
                     /locus_tag="VFU96_02455"
     CDS             complement(1686..2831)
                     /locus_tag="VFU96_02455"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5112148.1"
                     /translation="MTTQIELGFAPNEDPFVVAHGGFYRRWLSMLDDIDELKDLVSRL
                     EDTTEDKWVPIWAAAARRHEDEGDRLEAAGDFDGARRAYLQAKTYYAIGRFPGEISEV
                     KKTISQDCARAYLKACAHLDPPMEVVDFEHDGLKVRAHFRSPVADTPVPAVLIMCGAD
                     VFKEDRGWAQDLCMENGMASLVMDGPDTGENPFPWAPESVSAWEAAVDYLASRTEVDG
                     GRIGAFGISRGGYSVMQLAGTAPGKLGAVVANAGHPFGYVMPDDELEEFVRVRNERAT
                     WRFGAPDDPPSFPPTTMEKERSDFKRWALSEQGILDNITMPVLMINGKLDHLAPIGNI
                     YFMLETGPATGKEARVYADAGHCAFKYQSHWAPATFQWLAEKLNRQQ"
     gene            complement(2828..>3267)
                     /locus_tag="VFU96_02460"
     CDS             complement(2828..>3267)
                     /locus_tag="VFU96_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112149.1"
                     /translation="GGHTIKILSLAPYDCPDDLGEWDRGKDILAAEHFQALTDCAPNL
                     TEEKVLAWKAKSPVDIERGNPHMWRGTIHGGDRGLAYGGPQRPVPGWASHRMPIPGLY
                     QTGATTHPGGSITGGPGRNAAIVILDDLGMSLDSMVAREGGRS"
BASE COUNT          555 a         1085 c         1046 g          581 t
ORIGIN      
        1 ctgatcgccg acgagccgac caccgctctc gatgtgacca tccaggcgcg gatcctgaat
       61 ctgatcgcgg agctgcaaca gcgccttggg acgtcggtgc tctatatcag ccacgacctc
      121 tcgctggtgc gcgagatcag cgaccgggtc gcggtgatgt atgccgggag aatcgtcgag
      181 atcggcagaa ccgaagatct gttccgagag cccctccacc cctacacccg aagtctgatg
      241 tcggcgatcc catcgtcgac gagcacgagg ggccggctcg ccgccatcga aggcaacgtc
      301 ccagagctga tcgatccgca accatcctgc cggttccaca gccggtgccc tcatgcgttg
      361 gagatttgcc gcaacttcga tccacgtttc ctccgccacg acgtcgacca tggagtcgcc
      421 tgtcacggtc acgacgatcc cgccgatctc gggtttccca ccagtgaagt gccgaccgtg
      481 ggatcgatga gccgatgacg gatgtgcgag tcggggcgat caccactgag ccctacccca
      541 ggttctcgga cggagagctc caagcgaggc gccaggccat gctcgaggtc atggatggag
      601 ccggggtggc acacctggtc gcctacggat cggagcggac aggctcagca gtgcaatggc
      661 tctcggaatg gccggtcacg cgtgaggcgg ccctggtcct cagccccggc gagccgatcc
      721 gactgttggt ccagcattac aaccacctgc ccaacgctcg gaggatcgcc tctgactgcg
      781 acgtggagtg gggtggccca tccaccatta accggctcgg cgagctactc cgtgcgcgtc
      841 tgaccgacgg gcagcgcgtc ggggtggttg gcccgcttcg gtttcaagac caccgggacc
      901 tcgccgtatc ggtcggcgac ctggtgagtc tcgaccgtca gtatcagagg ctccgactgg
      961 tcaaatcagc ggaggaaatc gaacgtctcg aggtggcggc gcacctttcc gaccgggcgg
     1021 tggaggccat cgcggccgaa gcccgggtgg gcatggacga gcaccggctc gccgcgatca
     1081 tcgagtcggc ttatctcgcc gagggtggga ccaaccacat tcactacttt gccgcgaccc
     1141 cgatggccga tccgagcatc tgcgtgcctt cgcagttcca ttcgacgcgt cggctgcagg
     1201 ccggcgacat cctgttctgt gagatcagcg ccgcgtactg gggttacgcc ggccaggtgc
     1261 tacggagctt cgcgatcggc gccgacccga caccgctgta caacgacctg caccgggcgg
     1321 cagacggtgc cttcgatgcc atcgccgccg tgacccggcc cggcactcat gttcgagacc
     1381 tggtggacgc gtcctcggtg atcgaggaga tgggcttcac catctacgac gatctggtgc
     1441 acggctacgg gggcggatac ctgccaccgg tgctgggcag caggagccgg cccaatctcc
     1501 ccacgcccga catggtgctg gaagagggaa tggcgctggt gatccaaccc aacgtgatca
     1561 ccactgacga gagggcaggc gtccagaccg ggcatctcgt cgtggtcacc ggcgacgggc
     1621 accggccgtt gcacgacgtg cctcggggac tgaggcgaat cggctgagaa ccaacccggc
     1681 agggcctact gctgccggtt caacttctcc gccagccact ggaacgttgc cggcgcccag
     1741 tgcgactggt acttgaaggc gcagtggccg gcgtctgcgt acacgcgtgc ctccttgccg
     1801 gtagcgggcc cggtctcgag catgaaatag atgttcccga tcggagcgag gtgatcgagc
     1861 ttcccgttga tcatcagcac cggcatggtg atgttgtcga ggatcccctg ctcagagagc
     1921 gcccagcgct tgaagtcgga ccgttccttc tccatcgtgg tgggtgggaa ggaaggcggg
     1981 tcgtcggggg cgccgaatcg ccaggtagcc cgctcgttgc ggaccctgac gaactcctcg
     2041 agctcgtcgt cgggcatgac atatccgaat gggtggccgg cgttggccac gaccgctccg
     2101 agcttgcctg gtgcggtgcc ggcgagctgc atcaccgagt acccgccccg gctgatcccg
     2161 aaagcgccga tccgaccgcc atcaacctcg gttcgggaag cgaggtagtc gacagcggcc
     2221 tcccaagccg ataccgactc cggggcccac gggaacgggt tctccccggt gtccggcccg
     2281 tccatgacca gcgacgccat gccgttctcc atgcagaggt cctgagccca cccccgatct
     2341 tccttgaaca cgtcggcgcc gcacatgatg aggacggccg gcaccggggt gtctgccacc
     2401 ggcgagcgga aatgagctcg caccttcaac ccatcatgct cgaaatcgac cacttccatc
     2461 ggggggtcga ggtgagcgca ggccttcaga taggcccggg cgcaatcctg gctgatggtc
     2521 ttcttcacct cgctgatctc gccgggaaac cgcccgatcg cgtagtaggt cttggcctgg
     2581 aggtaggccc gcctcgcccc gtcgaaatcg ccggcagcct cgagccgatc accttcgtcc
     2641 tcgtgccgac gcgccgcggc tgcccagatc ggcacccatt tgtcctccgt ggtgtcctcc
     2701 agccgggaca ccaggtcctt cagctcatcg atatcgtcga gcatcgagag ccaacgccga
     2761 tagaacccgc cgtgggcgac gacaaagggg tcttcgttcg gggcgaaccc gagttcgatc
     2821 tgagtggtca tgaccgtcct ccttctctgg ccaccatgct gtccaggctc atccccaggt
     2881 catcgaggat gacaattgcg gcattgcgcc cgggccctcc ggtgatcgag ccaccaggat
     2941 gggtggtggc tcccgtctga tagagaccgg ggatgggcat gcgatgcgag gcccatccgg
     3001 gaaccggtcg ctgggggccg ccataggcga ggccacggtc gccgccatgg atggttcccc
     3061 gccacatgtg gggattgcct ctttcgatat cgaccgggct cttcgccttc cacgccagga
     3121 ctttctcctc ggtcagattc ggggcgcagt ccgtcaacgc ctggaaatgc tctgcggcga
     3181 ggatgtcctt ccctctgtcc cactcgccga ggtcatcagg acagtcgtac ggggccaggc
     3241 tgaggatctt gatggtgtgc ccgcctt
//
LOCUS       DASTXO010000119         3065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1259377, whole genome shotgun sequence.
ACCESSION   DASTXO010000119 DASTXO010000000
VERSION     DASTXO010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259377"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VFU96_02465"
     CDS             complement(<1..655)
                     /locus_tag="VFU96_02465"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU5112150.1"
                     /translation="MLPEKVRNVALVGHGGSGKTTLAEALLFVGGATSRRGTVEQGTT
                     VLDYEPEEIDRGISLGLGAATFNWNDHRINLIDTPGSSDFSGDARAALRAVDLALFVV
                     SGVDGVEVQTEQLWKVAEDEGIPRAVVVTKLDRDRSSFERTLDQLRDSFGKAIAPIQV
                     PIGSEEGLRGVVRVASERAYEYTEGEKSGKEVDLPAEVEALVHAAHTALVETVVETDD
                     "
     gene            742..1320
                     /locus_tag="VFU96_02470"
     CDS             742..1320
                     /locus_tag="VFU96_02470"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEU5112151.1"
                     /translation="MIDTRVRPRVTRFIEPIGKALASIGVTPTAMTAIGLATVILGSI
                     VISTGALRSGAAIVLVGSILDGLDGSVARAAGMVTARGAFLDSVFDRIGEIAAFAGLG
                     VAIARETDDEAVLLLIVLAIGGAMLVPYIRSRAEVEGFNGKAGLMGRAERVLLFTIGL
                     LLGFVEPMLWIFVILVWITALMRFIDTYRSFE"
     gene            1317..2195
                     /locus_tag="VFU96_02475"
     CDS             1317..2195
                     /locus_tag="VFU96_02475"
                     /inference="COORDINATES: protein motif:HMM:NF005919.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HEU5112152.1"
                     /translation="MSELPGYLALRAGAGLLSLLPASAARSLGRVGGRVWHATASGRR
                     SMVERHMTRVLGDQAEAIRAARSVMQSYGRYYAEALWARGSRVEELQAQTTVEGLENI
                     IGAREEGRGMIYGLPHVGNWEVAAPVSGNEKVPVVAVAENLPNRRITEWFTNMRADFG
                     IEIVLATGGTEVMRKLEAALSANKAVALLSDRDLKQRGVEVVFFGEKTTLPPGPATLA
                     LRTGAPLLPVASYYDGDDGYRVVIRPAIPVPTEGPRSEKVRAMTQILADEMESLIRAA
                     PRQWHLVQPNWPSDRE"
     gene            2192..>3065
                     /locus_tag="VFU96_02480"
     CDS             2192..>3065
                     /locus_tag="VFU96_02480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5112153.1"
                     /translation="MSPLRVGLVSPYDLSVPGGVQAQVLGLAAYLRSWGDDVVVIGPG
                     LPEGIAGVDLGESLSIPGNGSMVPIAADPRSRRRIRSAAAGLDLLHVHEPLMPLASLL
                     ATHAGPPVVGTFHAAPGRAGRLAYRLTRPLLSRLLGNTAMVTAVSRAAASVLPDGLEV
                     RLIPNGLDVESMKVETTRVPGRVAFLGRDEPRKGLDVLLEAWETVRTARPEAELVVMG
                     SKRDDAGPTWMGRVDEGTKAMVLSSSSIYVAPQTGGESFGIVLLEAMAAGAAVVASDL
                     ESFRDWAGEAALLVP"
BASE COUNT          486 a         1017 c         1018 g          544 t
ORIGIN      
        1 cgtcgtcggt ctcgacaacg gtctcgacca gggccgtgtg tgctgcgtgg accagggcct
       61 cgacctcggc gggcaggtcg acttccttgc ccgacttctc tccctccgtg tactcgtaag
      121 cgcgctcgga ggccacgcgc acgactcccc ggaggccctc ctcgctgcca atcggcacct
      181 gtatcggggc gatcgccttg ccgaacgagt cccgcagctg atccagggtc cgttcgaaag
      241 aagaccggtc gcggtcgagc ttggtcacca ctaccgctcg cgggatgccc tcgtcttcgg
      301 cgactttcca gagctgctcg gtctggacct ccacgccatc gacaccggac acgacgaaga
      361 gggcgaggtc gaccgcgcgg agggcggcgc gggcgtcccc agagaaatcg ctggagccgg
      421 gggtatcgat cagattgatc cggtggtcgt tccaattgaa ggtcgccgcg cccaatccga
      481 gcgagatgcc ccggtcgatc tcctcgggct cgtagtcgag gaccgtcgtg ccctgttcca
      541 cagtccctcg tctgctcgtg gcgccaccga cgaatagaag ggcttcggcg agtgtcgtct
      601 tgccgctccc gccatgaccg acgagggcga cgttgcgaac tttctcgggt aacaacatgg
      661 acctccactt cgcgcgagac gccgatccta accctgccct aggaccggac cacaaggccg
      721 ggcctgctac tttgctccgt cgtgatcgac acaagggttc gaccccgggt cacccgcttc
      781 atcgagccca tcggcaaggc tctcgcctcg atcggggtca ctccgaccgc catgaccgcg
      841 atcgggctcg ccaccgtcat cctcggctcg atcgtgatct cgaccggcgc attgcgctcc
      901 ggggcagcga tcgtcctcgt cggctccatc ctcgatggcc tcgacggctc ggtggcccga
      961 gccgcgggca tggtcaccgc ccgtggcgcg tttctcgact ccgtgttcga ccgcattggt
     1021 gagatagccg ccttcgccgg cctcggcgtg gccattgccc gcgagaccga cgacgaggca
     1081 gtcctcctgt tgatcgtcct cgccatcggc ggcgcgatgc tcgtccccta catcaggagc
     1141 cgggccgaag tcgaaggttt caacggcaag gccgggctga tgggccgcgc cgagagagtc
     1201 ctcctgttca ccatcgggtt gcttctcggc ttcgtcgagc cgatgctctg gatcttcgtg
     1261 atcctggtgt ggataaccgc gttgatgcga ttcatcgaca cgtaccgttc attcgagtga
     1321 gcgaacttcc cggctatctc gccctccgtg ccggcgcggg gcttctcagc ctgctgcctg
     1381 cctcggccgc tcgatccctg ggacgcgtcg gaggacgggt ctggcacgcc accgcgagcg
     1441 ggcggcgctc gatggtcgaa cgtcacatga cgcgggtcct gggcgatcaa gccgaggcca
     1501 tacgagccgc tcgttcggtg atgcagtcat acggccgcta ttacgccgaa gccctgtggg
     1561 ccagggggag cagggtcgag gaacttcagg cacagaccac cgtggagggt ctggagaaca
     1621 tcatcggggc cagggaggag ggcagaggga tgatctacgg gctgccccat gtcggaaact
     1681 gggaggttgc ggcgccggtc tcgggcaatg agaaggtgcc ggtcgtcgcg gtggcggaga
     1741 atcttcccaa ccgccggatc acggagtggt ttacgaacat gagagccgac ttcggaatcg
     1801 agatcgtcct cgccacgggt ggcaccgaag tcatgaggaa gctcgaggcc gcgctctcgg
     1861 ccaacaaggc cgtggccctc ctctccgatc gtgatctgaa gcagcgaggt gtcgaagtcg
     1921 tcttcttcgg tgagaagacc acactgcccc cgggcccggc caccctcgcc ctccgtaccg
     1981 gggccccgtt gctcccggtc gcctcgtact acgacggaga cgacggctac cgcgtcgtga
     2041 tcagacccgc tatcccggtg ccgaccgagg ggccccgcag cgagaaggtg cgggccatga
     2101 cccaaatcct cgcagacgag atggaatcgt tgatccgggc agcgccgcgg caatggcatc
     2161 tggtgcaacc caactggccg tctgaccggg aatgagccca ttgcgggtgg gcttggtcag
     2221 cccgtacgac ctctcggtcc ccggcggtgt ccaggcacag gtcctgggcc tggccgccta
     2281 tctccgcagc tggggcgatg acgtggtcgt catcggccct gggctccccg aggggatagc
     2341 cggtgtggat ctcggagaga gcctttcgat acccgggaac ggctcgatgg tcccgatcgc
     2401 cgcagaccct cgatcccgaa ggcggatcag atcagcagcc gccggccttg acttgctcca
     2461 cgtccacgag ccgctgatgc cgttggcctc cttgctggcc acccatgccg gaccaccggt
     2521 tgtcggcact tttcacgccg cccccggcag ggctggccgt ctcgcctatc ggctgacccg
     2581 cccgctcctg agccggctcc tgggcaacac cgcgatggtg acggccgtca gtcgtgctgc
     2641 cgcctcggtg cttcccgacg ggctggaagt gcgactcatc ccgaacgggc tcgacgtcga
     2701 gtccatgaag gtggaaacca ctcgggtgcc cgggagagtg gcgttcctgg ggagagacga
     2761 accgagaaag ggcctcgacg ttctgctcga agcgtgggag acggtcagga cggctcgacc
     2821 ggaggcagag ctcgtcgtca tgggatcaaa gcgggatgac gccgggccaa cgtggatggg
     2881 gagggtcgac gaaggcacca aggccatggt gctgagctcg tcgtcgatat acgtcgcgcc
     2941 tcagaccgga ggtgagtctt tcgggatcgt cctgctcgag gcgatggcgg ccggagccgc
     3001 cgtggtggcc tctgatctcg aatcgttccg tgactgggca ggggaggctg cgctgctggt
     3061 ccccg
//
LOCUS       DASTXO010000120         3841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1296275, whole genome shotgun sequence.
ACCESSION   DASTXO010000120 DASTXO010000000
VERSION     DASTXO010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296275"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VFU96_02485"
     CDS             complement(<1..428)
                     /locus_tag="VFU96_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112154.1"
                     /translation="MTETGPPGRQLWEEPDESHDAYDDFPPPPPVAPTRARPDMWTVV
                     LGVVGGALLGTGVTLAILGFTGVFEEPPVPTNPPPPSLTLPPPTTAPAVIADTGNATE
                     VAMRAIPSIIAVETSGALGEGGGSGVVYSADGYVITNHHVV"
     gene            443..877
                     /locus_tag="VFU96_02490"
     CDS             443..877
                     /locus_tag="VFU96_02490"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEU5112155.1"
                     /translation="MDAAHQSIRVSVGPAPIDVAKVVEEVGRPDSGATALFLGTVRDH
                     SHARSDVTHLEYEVYPELVEPKIRGIVEEAAERWPILSAVVEHRSGTVTLGEVSVAVA
                     VSTAHREDAFAAARFIIDELKTRAPIWKKEHWPGGSEWSAGS"
     gene            951..2828
                     /locus_tag="VFU96_02495"
     CDS             951..2828
                     /locus_tag="VFU96_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU5112156.1"
                     /translation="MTTVLERAGSPAADVGFKTPPAYVRDWAQERPDQVAMREKDFGI
                     WQEITWSQYWQSVLDAAHGLLVLGVYEGDRVSIHSEDRPEWVILDMATVAVRAITVGL
                     YPTNPAAEVEYLLSDSGAVVHLVEDQEQADKVMSVIGSLPDLRKIIHLEPRGFRKWRD
                     DDRFVSWDEFLEMGRSHRETNPGMVEQIMAQATGDDVITLVYTSGTTGPPKGAMLTNS
                     NFAFNADVLISVENRTPDGKPIGPDDLILTYLPLCHVAERIFSTWSSVSRGPSLNFAE
                     SIETVQQNLREVQPTIFFAVPRIWERIHASIAIKGRDGTWFKRLWFGIGMKLARRIGD
                     LRVANDGEHTMLSRFLYAIGYPIVFRAVKERIGLRRARYAASGAAPIAPEVLRDFLGM
                     GVPLFELYGMTENSAVATCNFPGANKVGTVGVPYPGTELRIDEATGEIQTRHPGVFKG
                     YWNKPEKTAEAFTEDGWLRTGDVGEWVDGTHVKIVDRIKDIIITSGGKNISPSEIENS
                     LKVSPFIKEAMVVGDGRKYLSALIAIESDVVGDWATRRQIPYTTYRDLTTKPQVIELI
                     QGVVNDANDKTSHVESIQKFRLIPKELDHEDGELTATQKLKRASMSSMFGDLIEDMYR
                     S"
     gene            2825..3799
                     /locus_tag="VFU96_02500"
     CDS             2825..3799
                     /locus_tag="VFU96_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU5112157.1"
                     /translation="MTTGLVVLAQETAGGSAFAQMLVDGISFGAIYALLAMGFVIIFK
                     ATSVINFAHGALTGLGAFLVVAFATVLDFPGRYLTFLPEWAQWTVNVLTAFVVAALLG
                     VLIERVFLRPMVGEALFAVAIVTLGVDIVIRTITNDWIGTQPRPVGAPWGLNVIDLGW
                     VRIAWSEVIQIAVTIVLLIAIAFFLRSRTGVAMRATAFDQETARAQGVNVGRVFSVAW
                     AIAAFLAAVAGLFYSVFPRRATGVDQATAFIAFRAFPAVIIGGLDSIPGAVVGGFAVG
                     IAESAANTYVTADFLGNGFPGIVGYLLMMVVLLVRPYGLLGTPEIRRV"
BASE COUNT          663 a         1203 c         1232 g          743 t
ORIGIN      
        1 accacgtgat ggttggtgat gacgtagccg tcggccgagt acacgacgcc gcttccacca
       61 ccctcaccga gagcgcccga ggtctcgacc gcaatgatgc tcgggatggc tcgcatcgcc
      121 acctcggtcg cattcccggt gtcggcgatg acggcggggg cggtggtcgg cgggggcaag
      181 gtcaggctcg gagggggcgg gttggttggc accggcggct cttcgaacac gccggtgaag
      241 cccagaatgg cgagcgtcac cccggtcccg aggagagccc cgccaaccac accgaggacg
      301 acggtccaca tgtcgggccg ggcccgggtc ggtgccaccg ggggcggcgg cgggaagtcg
      361 tcgtaggcgt catggctctc gtcgggctct tcccacagct gccgcccggg tgggccggtc
      421 tctgtcatag gcttgggatt ctatggacgc cgcccatcaa tcgatccgtg tctcggtggg
      481 gcccgcgccg atcgatgtcg ccaaggtggt cgaggaggtg ggtcggcccg attccggcgc
      541 cacggcactc ttcttgggga ctgtgcgcga ccattctcat gcccgatctg atgtcaccca
      601 cctcgaatac gaggtctacc ccgagttggt ggagccgaag atccgcggga tcgtggagga
      661 ggcggcggag cgctggccga tattgagtgc cgttgtcgag catcgctcgg gcaccgtgac
      721 cctgggggag gtttcggtgg cggtggccgt ctccaccgct caccgtgagg atgcgttcgc
      781 tgcggcccgt ttcatcatcg acgagctcaa gaccagagca ccgatctgga aaaaggagca
      841 ttggccgggc ggttcggaat ggagcgccgg ctcctagagc caaatttgat cagtccggca
      901 aagttcgatc ccacccgata ttgtccggtc gtctttgagg aggacacggg atgacgaccg
      961 tcctggagcg agccggctct ccggcggctg acgtcggttt caagacaccg cccgcctatg
     1021 tgagggactg ggcccaggaa cgtcccgacc aggtcgcgat gcgggagaag gacttcggca
     1081 tctggcagga gatcacctgg tctcagtact ggcaatcggt cctcgacgcc gcccacggtc
     1141 ttctcgttct cggcgtgtat gagggtgacc gggtctcgat ccattccgag gaccgtcccg
     1201 agtgggtgat cctcgacatg gccacggtgg cagtccgagc cataaccgtc ggcctctacc
     1261 cgaccaaccc cgccgccgag gtcgagtatc tcctttctga ctccggtgcc gtggtccatc
     1321 tggtcgaaga ccaggagcag gcggacaaag tgatgtcggt catcggctcg ctgcccgatc
     1381 tgcgcaagat catccacctc gagccccgcg gattccgcaa gtggcgtgac gacgaccgct
     1441 tcgtctcgtg ggacgagttc ctcgagatgg gtcgcagcca ccgggagacc aacccgggga
     1501 tggtcgaaca gatcatggcc caggcgaccg gagacgacgt gatcaccctg gtctacacct
     1561 cggggaccac cgggccaccc aagggcgcga tgctcacgaa ctccaatttc gccttcaatg
     1621 ccgacgtctt gatctcggtc gagaatcgga ctcctgacgg aaagccgatc ggccccgacg
     1681 acctgattct cacctatctg ccgctgtgtc atgtggcgga acgcatcttc tccacgtggt
     1741 cgtcggtgtc ccgaggcccc tcgctcaact tcgccgagtc gatcgagacg gttcagcaaa
     1801 accttcgcga ggtgcaacca acgatcttct tcgccgtccc ccgaatttgg gagcggatcc
     1861 acgcttctat cgccatcaag ggtcgggacg gcacgtggtt caagcggctc tggtttggga
     1921 tagggatgaa actcgcccgc cgcatcggag acctcagagt ggccaatgac ggcgagcaca
     1981 ccatgttgag ccggttcctc tacgcgattg gatatccgat cgtgtttcgg gcggtaaagg
     2041 agcggatcgg gctgcgtcgg gcccgctatg ccgccagtgg cgcggcgccg attgcacccg
     2101 aggtgcttcg tgacttccta ggaatgggtg ttcccctctt cgagctgtat ggcatgaccg
     2161 agaattcggc ggttgccacc tgcaacttcc ccggagccaa caaggtaggg acggttggcg
     2221 tcccttatcc agggaccgag ctccgcatcg acgaggcgac gggggagatc cagaccaggc
     2281 atcctggggt tttcaagggc tactggaaca agccggagaa gacggccgaa gcgttcaccg
     2341 aggatggctg gctgaggacc ggcgacgtcg gggagtgggt cgatggcacc catgtcaaga
     2401 tcgtggaccg gatcaaagac atcatcatca cctcgggtgg caagaacatc tcgccttccg
     2461 agatcgagaa ctccctcaag gtctcaccgt tcatcaaaga ggcgatggtg gtcggtgacg
     2521 ggcggaaata cctctccgcg ttgatcgcca tcgaatccga tgtggtcggc gactgggcaa
     2581 cgaggcggca gatcccctac accacctacc gtgacctgac caccaaaccc caggtcatcg
     2641 agctgatcca gggagtcgtc aacgacgcga acgacaagac ctcacacgtc gaaagcatcc
     2701 agaagttccg cctcatcccc aaggagctcg accacgaaga tggagagctg acggcgactc
     2761 agaagttgaa gcgggcctcg atgagctcga tgttcggtga tctgatcgag gacatgtatc
     2821 ggtcgtgacc accggcctcg tcgttctggc acaagagacg gcgggtggca gcgctttcgc
     2881 tcagatgctc gtcgatggga tcagcttcgg ggcgatctac gccttgttgg cgatgggttt
     2941 cgtgatcatc ttcaaggcga catcggtgat caacttcgcc cacggcgccc tgaccgggct
     3001 cggtgccttt ctcgtcgtcg ccttcgccac cgtcctcgac ttcccgggcc gctatctgac
     3061 gttccttccc gagtgggcgc aatggaccgt caatgtcctg accgccttcg tcgtcgccgc
     3121 cctgctcggc gtgctcatcg agcgcgtttt cctcagaccg atggtgggcg aggcgctttt
     3181 cgccgtggcc atcgtgaccc tcggcgtcga catcgtgatt cgtaccatca ccaatgattg
     3241 gatcggcact caaccccgtc cggttggcgc tccttggggc ttgaacgtga tcgatctggg
     3301 atgggtgcgg atcgcctggt cggaagtcat ccagatcgcc gtgaccatcg tcttgctgat
     3361 cgccatcgcg ttcttcttgc ggtcccggac cggcgtggcg atgcgagcca ctgcgttcga
     3421 ccaggagacg gcacgcgccc agggcgtgaa cgtcggacgt gtcttctcag tggcgtgggc
     3481 gatcgccgcc tttctggccg ccgtcgccgg gctcttctac tccgtgttcc cacgccgggc
     3541 caccggagtc gaccaggcca cggccttcat tgcttttcgc gctttccctg ccgtgatcat
     3601 cgggggactc gactccatac ccggggccgt cgtgggtggc ttcgccgtgg gcatcgccga
     3661 gtcggcggcg aatacttatg tgactgccga cttcctgggc aacggctttc ccggaatcgt
     3721 cggctatctg ttgatgatgg tggtcctcct cgtgcgcccg tacgggctgc tcggcacacc
     3781 ggagatacga cgggtatgag aggccgtccc cttctctaca cgagctacga ggccgaccaa
     3841 g
//
LOCUS       DASTXO010000121         2886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1222570, whole genome shotgun sequence.
ACCESSION   DASTXO010000121 DASTXO010000000
VERSION     DASTXO010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222570"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1816)
                     /gene="leuS"
                     /locus_tag="VFU96_02505"
     CDS             complement(<1..1816)
                     /gene="leuS"
                     /locus_tag="VFU96_02505"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEU5112158.1"
                     /translation="MSYDHRAIEAKWQDRWESDGVFHTTEDSTRPKFYNVAMYPYPSG
                     ELHIGHVRNYTYIDLITRYKMMQGFNVLSPMGWDSFGLPAENLAIETGIHPRVSVEAQ
                     ITRMKEHFRKLGAAFDWSRELASHDPAYYRWDQWLFLELYERGLAYKATAPVNWCPKD
                     QTVLANEQVVDGACERCGTLVEKRNLAQWFFKITEYADRLLDDLDRLDDWPDRVRTMQ
                     RNWIGRSEGARFKIPIKEMDASFEVFTTRPDTIFGMTFCVFAPEHPLVEELISGTGQE
                     EEVREYIVRAARETEIERLAEGEKTGVFTGRFAINPVNGREVPIFIADYVLMGYGTGA
                     IMAVPGQDQRDWDFAVKYGLDIVRTVDAGPDWDGDAFLGEGPTINSEFLDGLDRDGAK
                     QRIIAWLEQRGIGEKAIQYRLRDWLISRQRYWGCPIPMVECPACGLVPVPRDRLPVTL
                     PEVDDYAPKGRSPLAAVEDWVATTCPQCGGAASRETDTMDTFVDSSWYYFRYTDPHNA
                     DAIFDRAKADYWMPLDQYIGGVEHAILHLLYARFVTKFLFDIGLSPVTEPFMRMFTQG
                     MLVKDGAKMSKSKGNVVDPDHMIERFGADTTRLFALFAA"
     gene            1950..>2886
                     /locus_tag="VFU96_02510"
     CDS             1950..>2886
                     /locus_tag="VFU96_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5112159.1"
                     /translation="MKRMRWLALVAVLALIAGACASEGGDETSTTGGEEATTTAAGDT
                     TTSVATTEAPSELLTLDSGGCDYGGKINKIEATSETEVVFTLCAADPAFLVKIAFVPF
                     SVQPSEHLEATGGSPLENPVGTGPFMLPEGGWARGSEIVFEANPDYWGEAPAFDTLVF
                     RWREDSTARITELTSGNADFITNLAASDYAGIEGDANLQLLVDNNPNVFYVGFNNQFP
                     PFDNPQVRQAIALGIDRQRIIDTFYPEGSEVATHFTPCILENGCEGASFPDFDPEAAR
                     ALLAEAGFPDGFDTTITLRDVFRVYLPEPTAVAEDI"
BASE COUNT          546 a          905 c          904 g          531 t
ORIGIN      
        1 gcgcggcgaa caaggcgaac aggcgcgtcg tatccgcgcc gaagcgctcg atcatgtggt
       61 ccggatcgac gacgttgccc ttggacttgg acatcttcgc cccgtccttc accagcatgc
      121 cctgggtgaa catcctcatg aatggctcgg tgaccgggct cagcccgatg tcgaagagga
      181 acttggtgac gaagcgggcg tagaggagat ggaggatcgc atgctcgacc ccaccgatgt
      241 actggtcgag gggcatccag tagtcggcct tcgcccgatc gaagatggcg tcggcgttgt
      301 gggggtcggt gtacctgaag tagtaccagg aggagtcgac gaaggtgtcc atcgtgtcgg
      361 tctcacgact cgcggcaccg ccacactgcg gacatgtggt ggcgacccag tcttcgacgg
      421 cggcaagcgg agaacggccc ttgggggcat agtcatccac ctcgggcagg gtgaccggca
      481 gtcgatcacg cgggacgggg accagaccac acgcggggca ttccaccatc gggatcgggc
      541 atccccaata ccgctgtcgc gagatcagcc agtcgcggag ccgatattga attgccttct
      601 cgccgatccc ccgttgttcc agccaggcga tgatccgttg tttggccccg tcccggtcga
      661 gaccgtcgag aaactccgaa ttgatcgtcg gcccttcccc gaggaatgcg tcaccgtccc
      721 aatccgggcc ggcatcgacc gtgcggacga tgtccaaccc gtacttgacg gcaaaatccc
      781 agtccctctg atcctgaccg gggaccgcca tgatcgcccc ggtgccatag cccatcagca
      841 catagtcggc gatgaagatg ggaacttctc ttccgttgac cgggttgatg gcgaaccggc
      901 cggtgaagac cccggtcttc tcgccctcgg cgagacgctc gatctcggtc tcccgggcag
      961 cgcggacgat gtactcccgg acctcttcct cctggccggt gcctgagatc aactcctcca
     1021 ccagggggtg ctcgggggcg aacacacaga acgtcatccc gaaaatggtg tcgggccggg
     1081 tggtgaacac ctcgaaggag gcatccattt ccttgatcgg aatcttgaac cgcgcccctt
     1141 cggatcggcc gatccagttc ctttgcatgg tccggacccg gtcgggccag tcatcgagcc
     1201 gatcgagatc gtcgagcagc cggtcggcgt attcggtgat cttgaagaac cattgagcaa
     1261 ggtttcgctt ctcgacgaga gtcccacagc gctcgcaggc gccatcgacg acctgctcgt
     1321 tggcgagcac ggtttgatcc ttcgggcacc agttgaccgg cgcagtggcc ttgtaggcga
     1381 gaccccgttc gtagagctcg aggaagagcc actggtccca cctgtaatag gcagggtcat
     1441 ggctcgccag ctcgcggctc cagtcgaaag cggcacccag cttcctgaag tgctctttca
     1501 tccgggtgat ctgagcctcg acgctgaccc tcggatggat gccggtctcg atcgccaggt
     1561 tctcagccgg gagcccgaag ctgtcccatc ccatcgggct cagaacgttg aagccctgca
     1621 tcatcttgta gcgggtgatc aggtcgatgt aggtgtagtt gcggacatgg ccaatgtgca
     1681 gctcacccga cggatagggg tacatggcga cgttgtagaa cttgggccgg gtcgagtcct
     1741 cggtggtgtg aaacaccccg tcggactccc aacggtcctg ccatttggct tcgatcgccc
     1801 tgtggtcgta cgacatcgca gccaagggta attagcggcc gagaggtaac ccgacttgca
     1861 cgcgggccgt gggaaaccgg tagcgtgggc gccgtcgcgg ggatggttcg acttcgccgc
     1921 gacctaaccc aacaagggag gaagaagata tgaaacgaat gcgctggttg gcgctcgttg
     1981 cagtactcgc cctcatcgcc ggcgcttgtg ccagcgaggg cggtgacgag acgtcaacca
     2041 cggggggtga agaagcaaca acgacggcag ctggggatac gacgacatcc gtcgccacta
     2101 ccgaggcgcc gtcggaactg ctcaccctcg attctggggg ctgtgactac gggggcaaga
     2161 tcaacaagat cgaagccacc tcggagaccg aggtcgtctt caccctctgc gcggcagacc
     2221 cggcattctt ggtgaagatt gcattcgtcc cgttctccgt ccagccatcc gagcacctgg
     2281 aggcgacagg cggctctccg ttggagaacc cggtcggcac gggcccgttc atgctccctg
     2341 aggggggttg ggcacgtggc tccgagatcg tcttcgaggc gaaccccgac tactggggcg
     2401 aggctccggc gttcgacacg ctggtcttcc gctggcgtga agacagcacc gcacggatca
     2461 ccgagttgac atccggcaac gcggacttca tcaccaacct ggcggcgagc gactacgccg
     2521 gcatcgaggg tgacgccaat ctgcagttgc tggtcgacaa caacccgaac gtgttctacg
     2581 tggggttcaa caaccaattc ccacctttcg acaacccgca ggtgcggcag gcgatcgctc
     2641 taggcatcga ccggcagagg atcatcgaca ccttctaccc ggagggttcg gaagtggcca
     2701 cccacttcac gccatgcatc ctcgagaacg gctgcgaggg cgcatcgttc cccgacttcg
     2761 accccgaggc agcacgggcg ctactcgcag aagccggatt ccccgatggg ttcgacacca
     2821 ccatcacgct ccgtgatgtg ttccgtgtct atctcccgga gccgacagcg gtggccgagg
     2881 acattc
//
LOCUS       DASTXO010000122         3510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1296287, whole genome shotgun sequence.
ACCESSION   DASTXO010000122 DASTXO010000000
VERSION     DASTXO010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296287"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VFU96_02515"
     CDS             complement(<1..219)
                     /locus_tag="VFU96_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112160.1"
                     /translation="MHALLLAVFLAVLPPGGSFVDDDGNIHEGGIEAIAAEAITRGCN
                     PPANDRFCPDRTVTRGQMAVFLARALDLA"
     gene            complement(265..726)
                     /locus_tag="VFU96_02520"
     CDS             complement(265..726)
                     /locus_tag="VFU96_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5112161.1"
                     /translation="MADTTTPPNGPVVVGIDGSEPGFIALRHAAREAEWRDVALHVVH
                     VLDVSPAVLHLEGDRTIHTRELAESDRAEIWKQAEPILRDIEAEVIRVERDGDPGKAL
                     VAYSSEAHGSVLVVGPRGRGRVGRWLLGSTADQAVKSAMCDVLVVKPGAEV"
     gene            794..1687
                     /locus_tag="VFU96_02525"
     CDS             794..1687
                     /locus_tag="VFU96_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5112162.1"
                     /translation="MAAIEVEGLTKSYGDVVAVDGVDIKVESGEVFALLGPNGAGKTT
                     TVEILEGHRSADGGHITVLGFDPASGGRAYRERIGIVLQEGAVERELSVREALSIYGS
                     MYPRRLEADHLIDLVGLTEKAGARIKTLSGGQQRRLELALGLVGDPDLLFLDEPTTGF
                     DPSARRQAWGILENLTALGKTILLTTHYMDEAQHLADRIAVIDRGKIVAEGTPETLGG
                     RADGKSVIRFALPRGVGTDEIPVSSIRKDGDLIEVSADSATRALHELTTWAIGRGLEL
                     ERLQVTRPSLEDVYLELTGEH"
     gene            1677..2522
                     /locus_tag="VFU96_02530"
     CDS             1677..2522
                     /locus_tag="VFU96_02530"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5112163.1"
                     /translation="MSTDPVHRPSVASLVWQQFRYQNKVFWRTPIAAFFTIVFPLMLL
                     VLFTAIFGNEEIEGLGITTAQYFTPGLAVFAAVSATYTNLAIGTAIARDNGILKRIRG
                     TPIPPWAYIAGRVASSVYLAFLAMALMMAAGVVFYGVQIIARTLPAALLTFVVGVSCF
                     AALGMLVAAISPNGDATPAITNATLLPVAFISNIFFPIENPPRWMEIAGNFFPLKAFA
                     EAFRDCFDPTLTGSQFHWPQIGYLLLWGVVAALLAVRFFKWEPSAGSTSRRSRRRDES
                     KPVPA"
     gene            2497..>3510
                     /gene="mgrA"
                     /locus_tag="VFU96_02535"
     CDS             2497..>3510
                     /gene="mgrA"
                     /locus_tag="VFU96_02535"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HEU5112164.1"
                     /translation="MSRSLCPHDQEVIPLHDADPDRYSRMPYARSGRSGLYLPRISLG
                     LWHNFGGESLTEEGRRMILSSFDQGITHFDLANNYGPPPGSAERNFGALLASDLAPFR
                     DELVISTKAGYDMWAGPYGEWGSRKYMLASLDQSLGRMGLDYVDIFYSHRFDPDTPLE
                     ETLGSLDTAVRSGKALYAGISSYGPEKTREAHAILTRLGTPFIIHQPSYSMLNRWVED
                     GLLDVLGELGIGCIAFSPLAQGLLTDRYLDGIPEDSRVETGGALDRSMLSADNMARVR
                     ALNDIARERGQSLAQMAVAWVLRDERVTSALIGASRWSQIEDLLRAVDNTRFSDEELA
                     AIDR"
BASE COUNT          601 a         1175 c         1108 g          626 t
ORIGIN      
        1 ggcgaggtcg agggctcggg ccaggaacac ggccatctgg ccgcgggtga ccgtccgatc
       61 cggacagaag cggtcattgg ccggtggatt gcacccacgg gtgatggctt cggcggcgat
      121 ggcctcgata ccgccctcgt ggatattccc atcgtcatcg acaaacgagc ctccaggagg
      181 aagcaccgcc aggaacaccg ccaacaacag cgcatgcacc gctagtgaat cgactcgatc
      241 gtcatgcaac tcaaggcgta ccgcctagac ctcggccccg ggcttcacca ccaggacgtc
      301 acacatcgca ctcttgaccg cctggtcggc ggtcgagccg agcagccatc ggcccactct
      361 cccccgccct ctggggccga ctaccagcac ggatccatgg gcctcggatg agtaggcgac
      421 gagagccttg cccgggtcgc cgtcccgctc gacacgaatc acctcggcct cgatgtctcg
      481 gaggatgggc tcggcctgct tccagatctc ggccctatca ctctcggcca gctcgcgggt
      541 gtggatcgtg cggtcacctt cgaggtggag gactgccggg ctgacgtcga gcacatgcac
      601 cacatgcagc gcgacatcac gccattcggc ttcacgtgcg gcgtggcgca atgcaatgaa
      661 accgggctcg gaaccgtcga ttcccaccac caccggtccg ttgggaggtg tcgtcgtgtc
      721 cgccatcggc ggaatcctgc cagatgggaa cctgacagcc gaaccagatc gggcaacacc
      781 tagcatcggg cagatggcag cgatcgaggt cgagggcctg acgaagtcct atggcgatgt
      841 cgtggccgtc gacggcgtgg acatcaaggt cgagtcgggc gaggtgtttg ccctgctcgg
      901 gcccaacggc gccgggaaga ccaccaccgt cgagatcctc gaggggcacc gctccgccga
      961 tggcggccac atcactgtgc tcggcttcga cccggcgtcg ggtggccgtg cataccgcga
     1021 gcggatcggg atcgtcctcc aggagggcgc agtcgagcga gagctctcgg tgcgtgaggc
     1081 gctgtcgatc tacggaagca tgtaccccag gcgtctcgag gccgaccacc tgatcgatct
     1141 agtcgggctc accgagaaag ccggcgccag gatcaagaca ttgtcgggag gccagcaacg
     1201 gagactcgag ctcgcattgg gcttggtcgg tgatcccgac cttctcttcc tcgatgagcc
     1261 gaccacgggc ttcgacccgt cggcgcgacg gcaggcgtgg gggatcctcg agaacctgac
     1321 tgcccttggc aagacgatcc tgctcaccac ccattacatg gacgaggcac agcatctcgc
     1381 cgacaggatc gccgtcatcg accggggcaa gatcgtggcc gagggcactc cggagacact
     1441 cggaggacga gcagatggga agagcgtcat ccgattcgct ctgcctcgtg gagttggcac
     1501 cgacgaaatc ccagtcagct cgatcaggaa ggacggcgac ctgatcgagg tcagtgccga
     1561 ctcggcgacg cgtgccctcc atgagctcac cacctgggcc atcgggagag gcttggagct
     1621 cgagcggctc caggtgaccc ggccgtccct cgaggacgtc tatctggagt tgaccggtga
     1681 gcactgaccc cgtgcaccgg ccctctgtgg cctccctggt ctggcagcag ttccgttacc
     1741 agaacaaggt gttctggcgc accccgatcg cggccttctt caccatcgtg ttccccctca
     1801 tgttgctggt gctcttcacg gccatcttcg gcaatgagga gatcgaaggc ttggggatca
     1861 ccacggcgca gtacttcacc cccggtctcg ccgttttcgc cgctgtttct gccacctaca
     1921 ccaacctggc catcggcacg gccatcgccc gcgacaacgg gatactcaaa cggatcaggg
     1981 gaacgccgat ccctccttgg gcgtacatcg ccggccgtgt cgcgtcctcc gtctacctgg
     2041 cgttcctcgc catggccctg atgatggcgg ccggcgtcgt cttctacggc gtccagatca
     2101 tcgcccggac ccttccggca gccctactca cgttcgtcgt cggcgtcagt tgcttcgccg
     2161 cactcggcat gctggtggcg gcgatctcgc cgaatggcga tgccacgccc gccatcacga
     2221 acgccaccct tctccctgtg gcattcatct cgaacatctt cttccccatc gagaacccac
     2281 ctcggtggat ggagatcgcc ggcaacttct tcccgctgaa ggcctttgcc gaggcgttcc
     2341 gggactgctt cgatcccacg ctgaccgggt ctcagttcca ctggccccag atcggctatc
     2401 tcctcctgtg gggggtggtg gccgccctgc tcgcggtgcg gttcttcaag tgggagccgt
     2461 cagcggggtc gacctcgcgt cgtagtcggc ggcgggatga gtcgaagcct gtgcccgcat
     2521 gaccaggagg tgattccgtt gcacgacgca gacccggacc gctacagccg gatgccctat
     2581 gcccgatcgg ggcggagcgg cctctacctg ccgcgtatct ccttgggcct ctggcacaac
     2641 ttcgggggag aatcgctgac cgaggagggg cggcggatga tcctgagctc gttcgaccag
     2701 gggatcaccc atttcgacct ggccaacaac tacgggccgc cacccgggtc ggcggagagg
     2761 aacttcgggg cactcctcgc gtcggatctg gcgccgtttc gagacgagct ggtcatctcg
     2821 accaaggcgg gatacgacat gtgggccggg ccctatggcg aatggggctc tcgcaagtac
     2881 atgttggcca gcttggacca gagcctggga cgcatgggtc tcgactacgt cgacatcttc
     2941 tactcccatc gattcgaccc ggacaccccg ctcgaggaga cgttgggctc gctcgacacc
     3001 gcggttcggt cgggcaaggc cctgtatgca ggtatctcgt catatggccc ggagaagacc
     3061 cgggaggccc atgcgatcct cacccggctc ggaacaccgt tcatcatcca tcagccctcc
     3121 tattcgatgc tcaatcggtg ggtcgaagat gggctgctcg acgtcctcgg cgagctcggc
     3181 atcggctgca tcgcattctc cccactggcc caggggctgc tcaccgaccg gtacctcgat
     3241 gggatccccg aagactcccg tgtggagacg ggaggcgccc tcgatcgctc gatgctctcg
     3301 gcggacaaca tggcccgcgt gcgggcgctg aatgacatcg cgcgagagcg cgggcagtcc
     3361 ctggctcaga tggcagttgc ctgggtcttg cgcgacgaac gcgtcacgtc cgccctgatc
     3421 ggcgccagcc ggtggtcaca gatcgaagat ctgctccggg cggtggacaa cacgcgtttc
     3481 agcgacgagg agctggccgc catcgatcgg
//
LOCUS       DASTXO010000123         3778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1480655, whole genome shotgun sequence.
ACCESSION   DASTXO010000123 DASTXO010000000
VERSION     DASTXO010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480655"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..99)
                     /locus_tag="VFU96_02540"
     CDS             complement(<1..99)
                     /locus_tag="VFU96_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase"
                     /protein_id="HEU5112165.1"
                     /translation="MSGFLFPRTPSGRSSLIPPPPWHYSGEMLTVEY"
     gene            187..1566
                     /locus_tag="VFU96_02545"
     CDS             187..1566
                     /locus_tag="VFU96_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5112166.1"
                     /translation="MSGTLEPGHQERAELAESVLGVLQDWFDRADTMPASGVELDDEL
                     VARLMTPPNDEGRPLQAILAEVDEAGRSGVFHPSGGHLSYIPNAGLYTAALAEFLAAG
                     LNRYTGVSGAAPGMSAIEHGMVRWMASLFDMGTSASGLIVSGGSMANLTGVVAARTAR
                     LGDEFANGVIYVTRHTHHSLEKAARIAGFRKDQIRVVGIDVDLRMDPEALEAAIRDDR
                     AAGLDPFLVVGSAGTTDTGTIDPLDSLARIVADQALWLHVDAAYGGFFQLTGRGKAAL
                     SGIQQADSIAIDPHKGLSVPFGIGALLVRDEAHLIDANQGRGAYLREEDHTSGIRDIA
                     SLGPELTRPFRAMQMWLPLHLHGVAPFREALDTSLDLAEYAHRRLLDIDGLEVPWKPD
                     LSIVAFRFEDDSLGRLALETINRERVVHLSPTTIADRYVLRFAILNRRTTRDHLDHAI
                     DMIEKTLVG"
     gene            complement(1631..2050)
                     /locus_tag="VFU96_02550"
     CDS             complement(1631..2050)
                     /locus_tag="VFU96_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018854251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5112167.1"
                     /translation="MQTIDEVMTRNPLTVDETAPVSEAARMMRDSNIGDVLVTRSDGS
                     VCGIVTDRDLALAVVAEGNDPASMVVRDVCNHTIESVASSDPVGKAVKVMSERAIRRL
                     PVIDDGSLVGIVSLGDLAIERDPQSALANISEAPPNN"
     gene            complement(2069..2449)
                     /locus_tag="VFU96_02555"
     CDS             complement(2069..2449)
                     /locus_tag="VFU96_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112168.1"
                     /translation="MVNRIRLFLALEAAIFIVAALIHFEVIFDGYLDREAGTAESIIA
                     VVLLGGLLASMLRPAVTRRAGLIAQVFALAGTFVGVTLLFAVGPRTTLDVFIHLLMVV
                     VLVAGLVVTVRTPRSVSERVTSGI"
     gene            complement(2501..2917)
                     /locus_tag="VFU96_02560"
     CDS             complement(2501..2917)
                     /locus_tag="VFU96_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112169.1"
                     /translation="MNELDLNPLTEPIRPAGETSPALILLLWMVASAILAQAVLAGLF
                     ISATAPVLMAHAIIGSLLSWFAIAPAAVAIARRKDLDPRVVTGSILLPVGLWVQSTLG
                     HMPFAVGTAIHVPFGVGLFAASVILAIASARSGHVG"
     gene            3031..3738
                     /locus_tag="VFU96_02565"
     CDS             3031..3738
                     /locus_tag="VFU96_02565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5112170.1"
                     /translation="MIATPPRIARALFAPLAPGYERWARILSMGQDGRWRARMVQAIA
                     PRPGDTALDIAAGTGSITRLLQQKGASVISLDQSAPMLAPAVARGATGVLASAESLPF
                     PDARFDIVTFGYLLRYVGDVGECMTEISNALKPGGRVGMVEFGRPSGIWRPLWWLYTR
                     LVLPGAGLLAGEGWPEVGRFLGPNIEEFADRYPPEQLSAIWEEAGLSNVRFDRLSLGG
                     GLVMWADKPERSRVKGS"
BASE COUNT          680 a         1231 c         1242 g          625 t
ORIGIN      
        1 atactcgacc gtgagcatct cgcccgagta gtgccagggc ggcggcggga tcagcgagga
       61 ccgaccgctg ggagtgcggg gaaacaggaa acctgacatc agatcgtctg caagctgtag
      121 atcgcgtgat cgtatatggc caatccgggg tgggcgaagg gatcgcaccg ggctaccttg
      181 accccgatgt cgggaaccct cgagccgggg caccaggaac gcgccgagtt ggcggagagc
      241 gtcctcggtg tgctccagga ttggttcgac cgggccgaca cgatgcccgc gtccggagtc
      301 gagctggatg acgagctggt ggcgcgtctc atgaccccac ctaacgacga ggggcggcct
      361 ctgcaggcga tattggccga ggtcgatgaa gccggtcgct ccggggtgtt ccacccatcc
      421 ggcggccatc tctcctacat cccaaatgcc ggcctctata cggcggcact ggccgagttc
      481 ctcgccgccg gcctgaatcg ctacaccggg gtctcgggtg cggccccggg catgagcgcc
      541 atcgagcacg ggatggtgcg atggatggcg tcactgttcg acatgggcac atctgcctcg
      601 ggtctcatcg tgtcgggcgg ctcgatggcc aacctgaccg gtgtggtggc cgcccggact
      661 gcccggctcg gagacgagtt cgccaacggc gtcatctacg tcaccaggca cacccaccac
      721 tcgttggaga aggcggcccg gatcgctgga ttcagaaagg accagattcg ggtggtcggc
      781 atcgacgtcg acctgagaat ggacccggag gcgctcgagg cggcaatccg ggacgaccgc
      841 gccgctggac tcgacccgtt cctggtcgtc ggctcggctg ggaccaccga cacgggcacc
      901 attgaccccc tcgactccct ggcccgaatc gtcgccgatc aggcgctctg gctccatgtc
      961 gacgccgcct acgggggctt cttccaactg accgggcgcg gcaaggcggc cctctccggg
     1021 atacagcaag cggactcgat cgcgatcgac ccgcacaagg gcctgtccgt gcccttcggt
     1081 atcggcgccc tgctggtgcg tgacgaagct catctcatcg acgccaacca aggacgaggc
     1141 gcctacctgc gcgaggagga tcacacttcc gggatcaggg acatcgcctc cctcggcccg
     1201 gaattgaccc gcccgttccg cgccatgcag atgtggctcc cgcttcatct ccacggagtg
     1261 gccccgttcc gcgaggctct cgacaccagc ctggacctcg ccgagtacgc ccacagaaga
     1321 ctgctcgata tcgacggtct ggaagtccca tggaagccgg acctctccat cgtggcattc
     1381 cgcttcgaag acgactcgct cggtcgcctg gccctggaga cgatcaatcg ggagcgagtc
     1441 gttcacctct ccccgacgac gatcgcagac cgatacgtgc tccggttcgc catcctcaat
     1501 cggcgcacca ccagggacca cctggatcac gccatcgaca tgatcgagaa gacgctcgtc
     1561 ggctgacacc tggtcgaaac gtcagcagac ccgggcgatc gcccgggtct gcccaatccg
     1621 aataggttcg tcagttgttg ggtggagcct cgctgatgtt ggccaacgcc gactgggggt
     1681 cccgctcgat ggcgaggtcg cccagagaga cgatgcccac gagtgagccg tcgtcgatca
     1741 ccgggagccg ccggatggct cgctcgctca tcaccttgac ggccttgccc accggatcgc
     1801 tcgatgcgac ggattcgatc gtgtggttgc acacatcgcg aaccaccatc gaagccgggt
     1861 cattgccttc ggcaacgacg gccagagcga gatcacgatc ggtcacgatt ccgcagacgc
     1921 tcccgtcgga gcgggtcacg agcacatcac cgatgttgga gtcacgcatc atgcgcgccg
     1981 cctcactgac cggggcggtc tcgtcgaccg taagcggatt tctcgtcatc acttcatcaa
     2041 tggtctgcat ttgcacctcc tgttggtgtc aaatacccga tgtgacacgc tccgaaacgg
     2101 agcggggtgt ccgtacggtg acgaccaggc cggcgacgag cacgaccacc atgagcagat
     2161 ggatgaagac gtcgagcgtg gtccgcgggc ccacagcgaa caggagcgtc accccgacga
     2221 aggtccctgc cagggcgaaa acctgggcaa tcaatccggc gcgccgcgtg acggccggtc
     2281 gcagcatgct ggccaggagg cctccgagca ggaccaccgc aatgatgctc tcggcggtgc
     2341 ccgcctcccg atccaagtac ccgtcgaaga tgacttcgaa atggatcagg gccgccacga
     2401 tgaatatggc cgcctccagt gcgaggaaaa gccggattcg attgaccatc tctcccccat
     2461 tccacgaaag cgggtgttct caaaccaggc cccgcgaggt tcagccgacg tggccggacc
     2521 tggccgaggc aatggcgagg atcacggagg cggcgaatag gccgacaccg aaggggacgt
     2581 gaatcgcggt gccgacggca aacggcatgt gcccgagcgt cgattgcacc cacagcccaa
     2641 cgggcaggag aatcgacccg gtgacaaccc gaggatcgag atccttgcgc cgggcgatgg
     2701 cgacggctgc cggagctatg gcaaaccatg agagaaggga tccgatgatg gcgtgagcca
     2761 tcagaaccgg cgccgttgcc gagatgaaaa gaccggccag cacggcctgg gccaggatcg
     2821 ccgaggccac catccagagg agcaagatca gggcggggga tgtttctcca gcagggcgga
     2881 tgggctccgt cagagggttc aggtcgagtt cattcatggg atcctgaaag tacgggaaac
     2941 ctgtcgaccg gtcgtcggcc gggggttgtc gatcaggtac cgccggggag gtatgaccgg
     3001 caccgccctt ggatcggtac cgtcccgtcc gtgattgcca ctccgccccg catcgcgcgg
     3061 gcgctgttcg cccccctcgc ccccggctat gagcgatggg cccggatcct gagcatgggg
     3121 caggacggtc gctggcgtgc ccgaatggtg caggcgatcg caccccgtcc cggggacacg
     3181 gcgctcgaca tcgctgcagg cacaggctcg atcacccgac tccttcagca gaagggcgca
     3241 tccgtgatat ccctcgacca gagcgcgccg atgctggcgc ccgcggttgc caggggcgcg
     3301 accggcgtgc tggcgtcggc agagtcgctc ccgttccccg atgcccggtt cgacatcgtc
     3361 actttcggct atctactccg ctacgtcgga gacgtgggcg agtgcatgac cgagatcagc
     3421 aatgccctga aacccggggg ccgggtcggg atggtggagt tcggccggcc ttcgggcatc
     3481 tggcgaccct tgtggtggct ctacacccgt ctggtgttgc ctggcgccgg gctcctggcc
     3541 ggtgagggct ggcccgaggt agggcgattc ctcggcccca atatcgagga attcgccgat
     3601 cgctatcccc ctgagcagtt gtctgcgatc tgggaggaag cgggactctc caatgtccgg
     3661 ttcgatcggc tgtccttggg cgggggcctc gtgatgtggg cggacaaacc ggagcgatcc
     3721 cgggtgaagg ggtcgtgagc gatcggccag ctttctacgc gccgaccggc tcgcttac
//
LOCUS       DASTXO010000124         7293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1517377, whole genome shotgun sequence.
ACCESSION   DASTXO010000124 DASTXO010000000
VERSION     DASTXO010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7293
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517377"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VFU96_02570"
     CDS             complement(<1..678)
                     /locus_tag="VFU96_02570"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU5112171.1"
                     /translation="MTDARIANRAILRLAIPALGTLAIDPLLTLIDTAFVARVGVDEL
                     AALGVDTAILGFVFFGFNFLAYATTPLVAQALGRGNREEARRWVGDAFVLAALLGVVA
                     AIVVEVLAPWFVGLMGAGEDVAGPAIAYLRIRGIATPAVLLVTAGHGAFRGYQDTRTP
                     FRVAVGINVVNLVVDPLLIFVAGLGLEGAAIGTVIAQWVGALWFLHLIRSRRMAERPA
                     GLRKALPT"
     gene            complement(675..1370)
                     /gene="nth"
                     /locus_tag="VFU96_02575"
     CDS             complement(675..1370)
                     /gene="nth"
                     /locus_tag="VFU96_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010872392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU5112172.1"
                     /translation="MSLNKADGSIPSRADKGKARAILNRLRRRYPDIGTALDYESPFQ
                     LLVVTVLSAQTTDENVNKVAPVLFEAYPTVTDLAEADPEEVEKIVYSTGFFRQKTKSI
                     IKLAQGIEEMFDGEVPMDLDELVKLPGVGRKTASVLLAEAWDVPAIAVDTHVRRVARR
                     LGLTDQADPVKIERDLMAQYPKGDWSGLSMRIIQFGRDVCDARRPLCGQCELFGLCEF
                     PDRFVFAHKPPPG"
     gene            1448..2287
                     /locus_tag="VFU96_02580"
     CDS             1448..2287
                     /locus_tag="VFU96_02580"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU5112173.1"
                     /translation="MFRSKRSRAILALLAASFVFGATFVAWRFLLGALLLSIFALPRG
                     RTIWWHGTLAGLALFCGYAFQTAGLTETSASNSALITGLYVVITPFLAALFARRPPTW
                     WAVGAAAISFAGLILITGADGLSFERGDLLTLACAFSFSMHIVILSRLARHHPVVPFT
                     AVQLAVTSALAFPAALIIEGAVLPPTSVWGALLITGIGASAGCFVLQIWAQTVVGATT
                     AAVVLAAEPAFAVATAWVVLDERLTAAGWAGAALILIAIFIVVTKQTDQASIEAEAVT
                     PAH"
     gene            complement(2281..2559)
                     /locus_tag="VFU96_02585"
     CDS             complement(2281..2559)
                     /locus_tag="VFU96_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112174.1"
                     /translation="MAVFLVRLGVFVLSAAAIGIALIPILVLIDLLDGGTGWGLCPQG
                     IEACNNPYTTAAEFGILLTVGLFLSVLGIRLLMKLARRLRSDDYQVSQ"
     gene            2615..4237
                     /locus_tag="VFU96_02590"
     CDS             2615..4237
                     /locus_tag="VFU96_02590"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEU5112175.1"
                     /translation="MAPYHQEEFTTDEQAVLRRFFTNLDLPVFGLINLPEVVKGALFA
                     RYSRSAKSLRRLFLDEFVADPESGIGTMAQGLDEDPLVSTRKAEQLYQRVFFDYGDDS
                     VAQLGGAHLACEQASNLLTKVLEWGRLAAYLEQSTRYIRYDARLGDRYRYHVPTEISE
                     SRHAARFLTHMERVFDTYSSMVAQMMTHFEEEHPRGEDDSTMVWNATIRAKACDTVRG
                     LLPVSTLSNVGIYGTGQAYEMSLVRMRANPLREVQEYGEMMLGELRKIIPSFLTRVDL
                     DDRGVRWARYLEDVRSDLEQRANDLPFPSDLGPSVTLVDWDPEAEVKVTAAALYAVSE
                     LPDAELLRIARSMTQSERAGVIGSLVGDRANRRHKPGRAMERTTYRFDVTCDIGAFRD
                     LQRHRLMTLEWQRYTTRLGYDLPGKVEELGLTDEWDRIMAQSLTFYEELRKDLGPDVA
                     QYVVPFAFNVRFVMEMNPRQAFHLIELRSQAAGHPAYRRVAHEMHRSIRDVAGHHLIA
                     DAMSFVDYSDVDLERLESERQSERRRERARTT"
     gene            4270..4488
                     /locus_tag="VFU96_02595"
     CDS             4270..4488
                     /locus_tag="VFU96_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112176.1"
                     /translation="MAQFDVDAMLLRYQERAAAVKERPLPPVAGDERRLFVERAEADY
                     TDYALIGSASWSVEDGELVLRIPLGGPG"
     gene            4501..5346
                     /locus_tag="VFU96_02600"
     CDS             4501..5346
                     /locus_tag="VFU96_02600"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HEU5112177.1"
                     /translation="MLEKRLVLVSGKGGVGKSAVSAGLALLAQRRGLRVLAMEMGSPG
                     GLSAHFGTGALEFEPTELRPGLHAMQMVRSDALLEYLSLQLNIKGLTRFGPLARAFDA
                     LATTAPGIREIITIGKAIWEVREDRWDIVIADAPPTGQIGSFLRAPLTIAELVPSGRI
                     RIQAEIMQEILRDPSVTGMFLVTLPEELPATETSETISWLAKEDVVAPPIVMANRVLD
                     ELTDPSTPPGPVGEAARLHRALFFEQQVWLDRLPPDHILPYLHGLFTPGEVAARIADE
                     LELIT"
     gene            5343..6377
                     /locus_tag="VFU96_02605"
     CDS             5343..6377
                     /locus_tag="VFU96_02605"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HEU5112178.1"
                     /translation="MTDTLLITGAGGVGKTTVAAAIGVRAARKGLRTLVVTVDPARRL
                     ATALGVDELGNEPQPHAEEPNLWAAMLDATASWRAVAMRHADPEVATRLADNDFFRAA
                     SEHFPASQSYAAADQAATFVQAMAWDLVIVDTPPSGGGIDFFAAPAQMADLVGGRLLR
                     WLTGGPLPGRRTFYNLAAKPVLRIADSVLGSDLLERVAEFLLDLRTTYDGVAQRGREI
                     ERVLTSASVFVVTTSDPAPVREAIKFYRDLPGLASSPAAVVFNRILPEDWADATAPPG
                     TSLALEQNLARWSAETVRQRDTREEFSSRYGAHIATIPWSSSPPTDLDGLARLLDDSK
                     ELPWDRVGIS"
     gene            6382..7191
                     /locus_tag="VFU96_02610"
     CDS             6382..7191
                     /locus_tag="VFU96_02610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEU5112179.1"
                     /translation="MANSAAFFDLDRTLISGASAFPLGVEAWRQGLASNPDIGRWALS
                     ALSFRLMGDKGDATDDVKGDFLSRIAGSSVQALEMVGAAVLPKLVARVRPESRKLINL
                     HHEAGRETWIVSAAPHEIVEPLARSLGMTGAIGTRGKVVAGHYTGELDGPFVYGAGKV
                     DAIEKIAADLGYDLERSYAYSDSVSDLPMMEAVGHPVAVNPDSELDAVAHERGWPVVI
                     FARKTKRAVAVSALASLGVSAAVAAYALGRKHGRSSALAQMAQREIWRQRT"
BASE COUNT         1278 a         2401 c         2311 g         1303 t
ORIGIN      
        1 cgtggggagg gcctttctga gacccgcggg cctttcggcc atgcgtcggg agcggatcag
       61 gtgcaggaac cacagcgccc cgacccactg ggcgatcacc gttccgatgg ccgccccctc
      121 gaggcccaag ccggcgacga agatcaggag aggatcgacc accagattga ccacgttgat
      181 cccgaccgcg acgcgaaagg gcgtccgcgt gtcctggtag ccgcggaaag cgccatgacc
      241 ggcagtgacc aggagcaccg ctggtgtggc tataccgcgg atccggagat aggcgatggc
      301 gggcccggca acgtcttccc ccgctcccat caagccgaca aaccacggcg cgaggacctc
      361 gaccacgatc gcggcgacga cgccgagcag ggcggcgagg acgaaggcgt ctccgaccca
      421 ccggcgtgct tcttcccggt tcccacgtcc aagtgcttgc gccaccaagg gcgtcgtggc
      481 gtaggcgagg aagttgaatc cgaagaagac gaatccgagg atcgccgtgt ccacccccaa
      541 ggccgccagc tcgtcgactc caacccgcgc gacgaaggcg gtgtcgatca acgtcaggag
      601 tgggtcgatt gccagcgtcc ccagagccgg gatggcgagc cggaggatgg cccgattggc
      661 gatacgggcg tcggtcaccc cggaggtggt ttgtgggcga agacgaaccg atcgggaaac
      721 tcacagagac cgaagagctc gcactgcccg cacaacggcc gtcttgcgtc acagacgtca
      781 cgcccgaact ggatgatccg catcgaaagg cccgaccagt cgcccttggg gtactgagcc
      841 atcaggtctc gttcgatctt caccggatcg gcctgatcgg tcaggcccag acggcgtgcg
      901 accctccgca cgtgagtgtc gaccgcgatc gcaggcacgt cccacgcctc ggcgagcaac
      961 acgctggctg tcttgcgacc aacgccaggg agcttgacca gctcgtcgag gtccatgggc
     1021 acctcaccgt cgaacatctc ctcgatcccc tgggccagct tgatgatcga cttggtcttt
     1081 tgccggaaga agccggtcga gtagacgatc ttctcgacct cctcaggatc ggcctcggcg
     1141 aggtcggtga ccgtgggata ggcctcgaag agaaccggag ccactttgtt gacgttctca
     1201 tccgtcgttt gcgccgacag gacggtgacg acgaggagct gaaacggaga ctcgtagtcg
     1261 agggcagtgc cgatgtccgg atagcgacgg cggagacgat tgaggatggc gcgtgccttc
     1321 cccttatcgg cccgagacgg gatcgaccca tcggctttat tgagcgacat gggccggaat
     1381 cgtattgagc agcgcacccg ggagacgcac gagcgccacc cggcccggtc ctaccctgcc
     1441 tccgctcatg ttcaggtcca aacgaagccg agcgatcctt gctttgctgg cggcatcctt
     1501 cgtcttcggc gcgaccttcg tggcctggcg cttcctgctg ggtgcgcttc tcctctccat
     1561 cttcgccctt ccgcgtgggc gaactatctg gtggcacggc actctggccg ggctcgccct
     1621 gttctgcggc tacgccttcc agaccgcggg cctgaccgag accagcgcca gcaattcggc
     1681 cctgatcacc ggcttgtacg tcgtgatcac gccgttcctg gccgccctgt ttgcacgccg
     1741 gccgccgacc tggtgggctg tgggtgccgc ggcaatatcc ttcgcagggc tcatccttat
     1801 caccggagcc gatgggctga gcttcgagcg cggggatctc ctcaccctgg cctgcgcgtt
     1861 ctcgttctcg atgcacatcg tgatcctctc ccgcctcgcc cgtcaccacc cggtggtccc
     1921 gttcacggcg gtccaactcg ccgtcacatc ggctttggca ttccctgctg cgctcatcat
     1981 cgaaggggcc gttctgcccc cgacctcggt gtggggagcg ctgttgataa cggggatcgg
     2041 cgccagcgct ggatgcttcg tgctccagat ctgggctcag accgtggttg gtgcaaccac
     2101 agcagccgtg gtgctcgccg ccgagcctgc gttcgcggtc gccacggcgt gggtggtcct
     2161 ggatgagagg ctcacggcgg ccggctgggc cggcgcggcc ctcatcctga tcgcgatctt
     2221 catcgtggtg accaagcaga ccgaccaggc gtcaatcgag gccgaagcgg tgactcccgc
     2281 tcattgactc acctgatagt cgtcggaccg gagacgtctt gcgagcttca tcaagagcct
     2341 tatccccagc accgacagga acaaccccac agtgaggagg atcccaaact cggcagctgt
     2401 ggtgtagggg ttattgcagg cttcgatccc ttgaggacag agcccccatc cggtcccccc
     2461 gtcgagcaga tcgatgagga cgaggatcgg gatcaatgca ataccgatgg cagccgccga
     2521 gaggacgaac acaccgagac gcaccaagaa gactgccact gccctgaaac ggtaaccggg
     2581 cgtcttcagt ttccaggcat acaatcgccg acccgtggcg ccgtatcatc aggaggagtt
     2641 caccaccgac gaacaggccg tcctgcgccg gttcttcacc aatctcgacc tccccgtctt
     2701 cgggctgatc aacctccccg aggtggtcaa gggggcgcta tttgcgcgct actcgaggtc
     2761 ggccaagtcg ctgcgacggc tctttctcga cgagttcgtg gccgatccgg aatccggcat
     2821 cggcacgatg gcccaagggc tcgatgagga tccgttggtg tccacgcgta aggccgagca
     2881 gctatatcag cgggtcttct tcgactacgg agacgactcc gtcgcccaac tgggtggcgc
     2941 tcatctcgcc tgtgaacagg cctcgaatct cctgaccaag gtattggagt ggggcagact
     3001 cgccgcctac ctcgagcagt cgacccgcta catccgatac gacgcccggc tgggggatcg
     3061 ctaccggtat cacgtcccga ccgagatctc cgagtcccga cacgccgctc gcttcctcac
     3121 ccatatggaa cgggtcttcg acacgtattc gagcatggtg gcccagatga tgacccactt
     3181 cgaggaggag cacccccggg gtgaggacga ctcgacgatg gtctggaatg cgacgattcg
     3241 agccaaggcg tgtgacacgg tgcggggctt gctcccggtg tcgacgctct ccaacgtcgg
     3301 catctacggg accggccagg cctacgagat gtccctggtc aggatgcgag ccaacccgct
     3361 gcgcgaagtg caggagtacg gcgagatgat gctgggcgag ctccgaaaga tcatcccatc
     3421 gttcctcacc agggtggatc tggatgaccg cggggtccgc tgggcccgat acctggagga
     3481 cgtgcggtcc gatctcgagc aaagggcaaa cgacctgccg ttcccctccg acctggggcc
     3541 atcggtgaca ctcgtcgact gggatcccga agcagaagtc aaggtcacgg ccgctgccct
     3601 ctatgcggtc tcggagctac cggatgctga gttgctccgc atcgccaggt cgatgaccca
     3661 atccgagcga gccggggtca tcggctcgct cgtcggtgac cgggccaatc gacggcacaa
     3721 acccgggcgg gccatggagc ggaccacata ccgattcgac gtgacgtgtg acatcggcgc
     3781 ttttcgcgac ctccagcgtc atcggctcat gacattggaa tggcagcggt acacgacccg
     3841 tctcggctat gacctgccgg gcaaggtcga agaattgggg ctcaccgacg aatgggatcg
     3901 gatcatggct cagtcgctca ccttctacga ggagttgaga aaggacctcg gcccggacgt
     3961 ggctcagtac gtcgtgccgt tcgccttcaa cgtgcgcttc gtcatggaga tgaacccgag
     4021 gcaggctttc catctcatcg aattacgcag ccaggcggcc ggacatcccg cgtatcggcg
     4081 ggtcgcccac gagatgcacc ggtccatccg ggacgtggcc ggacaccatc tgatcgccga
     4141 tgcgatgagc ttcgtcgact acagcgacgt cgacctggag cgattggaga gtgagcgtca
     4201 atccgagcgg cgccgggagc gggcacgcac gacttagaac gtgggacgta gagcgtataa
     4261 cgtcttctca tggcccaatt cgatgtcgat gccatgctct tgcgctatca ggagcgggct
     4321 gccgcagtta aggagcggcc cctccccccg gtggcaggcg acgagcgcag actcttcgtg
     4381 gagcgggcgg aggccgacta caccgactac gccctcatcg ggagcgccag ttggagcgtg
     4441 gaggatggcg agctcgttct ccgcataccg ctcggcgggc cgggctagcc agctgggccg
     4501 atgctggaga aacgtctcgt tctcgtctcg ggcaaaggag gggtgggcaa atcggcggtg
     4561 agcgccggcc tcgccctgct cgcccaacgc cgtggcctgc gggtgctggc catggagatg
     4621 ggatccccag gtggcctctc ggctcatttc gggactggcg ccctcgaatt cgagccgaca
     4681 gagctgaggc ccgggctcca tgccatgcaa atggtccgct ccgatgcgct cctcgaatat
     4741 ctctcccttc agctcaacat caagggtctg acgagattcg ggcccctggc acgggcattc
     4801 gacgccctgg cgaccacagc ccctggcatt cgcgagatca tcacgatcgg caaggcgatc
     4861 tgggaggtac gggaagatcg atgggacatt gtcatcgccg atgccccgcc cacgggtcag
     4921 atcgggtcct ttctccgggc tccgttgacc atcgccgagc tcgtgccctc cgggcggatc
     4981 cggatccagg cggagatcat gcaggagatc ctccgtgatc cttcggtcac gggaatgttc
     5041 ctcgtcaccc tccccgagga gctgcccgcc acggagacga gcgagaccat cagctggctg
     5101 gcgaaggaag acgtggtggc accgcccatc gtcatggcga accgagtcct cgacgagttg
     5161 accgacccga gtacgccacc gggtccggtc ggagaggcgg cacggttgca ccgcgccctc
     5221 ttcttcgagc agcaggtatg gctcgatcgg ctcccgcctg accacatact cccctacttg
     5281 catgggctct tcacacccgg tgaagtcgca gcccgaattg ccgacgagct cgagttgatc
     5341 acatgacaga tactttgctg atcacaggtg ccggaggcgt cggcaaaacc actgtcgcgg
     5401 ctgcgatcgg ggtcagggct gcccgcaagg ggttgcggac gctcgtggtc accgtcgacc
     5461 cagctcgcag gctggccact gctctgggcg tcgacgaact cggcaatgag ccccaacctc
     5521 atgccgaaga gccgaatctg tgggcggcga tgctggacgc caccgcctcg tggcgcgcag
     5581 tggcgatgag acacgccgat cccgaggtag ccacccgtct cgctgacaac gacttcttca
     5641 gggcggcgtc ggagcatttc ccggcctctc agtcctatgc ggcggccgat caggccgcga
     5701 ccttcgtcca ggcgatggcc tgggacctcg tcatcgtcga tacgcctcca tctggtggcg
     5761 ggatcgactt ctttgcagcc cctgcccaga tggccgatct cgttggaggc cgtctcctcc
     5821 gatggctgac cggtggcccc ctccccggac gtcgcacttt ctacaacctg gcggcgaagc
     5881 cggttcttcg catcgccgac tcggtgctcg gatctgatct gctcgaacgc gtcgctgagt
     5941 tcctcctcga tctgcggacc acctatgacg gtgtggcgca acgtggccgg gagatcgagc
     6001 gtgtcctgac ctcggcgtcg gtgttcgtcg tcacgacctc ggaccccgct ccagtgcgcg
     6061 aagccatcaa gttctatcgc gacctcccgg gactggcgtc gagccctgcc gcagtcgtct
     6121 tcaaccgcat ccttcccgag gattgggcgg acgccaccgc tcccccaggc acatcactcg
     6181 ccctggagca gaacctggcc cggtggagcg ccgagacagt gcgccaacga gacacacgcg
     6241 aggagttctc ctcacggtac ggagctcata tcgccaccat cccctggtcg tccagccctc
     6301 cgaccgatct cgatgggttg gcccgcctcc tcgacgactc caaggagttg ccttgggacc
     6361 gggtgggcat atcctgatca catggctaac tcggccgcat tcttcgatct cgaccggaca
     6421 ctgatctccg gcgcttcggc gttcccactg ggtgtcgaag cttggcgtca aggcctcgcg
     6481 tccaacccgg acatcggccg ctgggcgctg tcggcgttga gcttcagatt gatgggtgac
     6541 aagggtgacg ccaccgacga cgtcaagggt gacttcttga gccgcatcgc cgggtcgtcg
     6601 gttcaggcgc tcgagatggt tggcgccgcc gtcctcccga agctcgttgc ccgggtccga
     6661 ccggagtcgc gaaagttgat caatctccat cacgaggcag gaagggagac ctggatcgtc
     6721 tcggccgcac cccacgagat cgtcgagccc ctggctcggt cgctcgggat gaccggtgcc
     6781 atcggcacga ggggaaaggt cgtggcgggc cattacaccg gcgagctgga cgggccgttc
     6841 gtatatggcg cgggaaaggt cgacgccatc gagaagatcg cggccgacct cggctacgac
     6901 ctcgagcgca gttatgccta ctccgactcc gtctccgatc ttccgatgat ggaggccgtt
     6961 gggcatcccg tcgcggtcaa ccccgattcc gagctcgacg cggtggccca cgaacgaggc
     7021 tggcctgtcg tgatcttcgc ccgcaagacg aagagagcgg tggctgtcag tgccctggcc
     7081 agtctgggtg tgtcggccgc agtcgccgcc tacgccctgg gacggaagca cgggcgatcc
     7141 tctgccctgg ctcagatggc gcagcgggag atctggcgcc agcggaccta gagaatcgca
     7201 ctcggcccga ggagctcttt caactcggca aagagcgccg tccgtggctc gacacgatgg
     7261 gcatcgctca gtttgagcac cttgactcct tgc
//
LOCUS       DASTXO010000125         2005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_265176, whole genome shotgun sequence.
ACCESSION   DASTXO010000125 DASTXO010000000
VERSION     DASTXO010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265176"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="VFU96_02615"
     CDS             complement(<1..844)
                     /locus_tag="VFU96_02615"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5112180.1"
                     /translation="MRDWAAKHRWESILIVVLAATIVVNVSLSPFYLGFGNFVNLFWL
                     SIEKIIVVVVMTFVIINGEIDLSVASVMGFSAAVMATLYEGGELPFALAILIGIAVGA
                     IAGLLQGLVITRFGLPSLVVTLAGLIGFRGAARVLLEDRSVGDFPEWFESIGQQTLVG
                     PFPFALVLFFVGLALAWVILERSAFGRKVYVIGNSADVARFSGIEVDSVKVRLFVSSG
                     AVAGLAGILFAARVGAVQGTLGETYELDIITMVLLGGVSIFGGSGSMLGVVLAMFTVL
                     NIRNG"
     gene            complement(837..1940)
                     /locus_tag="VFU96_02620"
     CDS             complement(837..1940)
                     /locus_tag="VFU96_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010150714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5112181.1"
                     /translation="MTESTTVAPIRSTRFLRPERVKEATLLAIIAVSIVVFAFLIEGY
                     LSGRFFNRVTTSVVVVTLLAAAQALVIFSRNIDLSVGSIVGVAAYLTGDFLAATGQGP
                     IPAMLVAAVVGAVLGSVNGLLVAYGGVPAIIVTLGTLALFRTLLSIYSEGANVLAGDL
                     PSWVLEFNNVTLFTIAGFEVRLVFVIAVVVVVGLQFALARLRWGRRIYAIGSNPSAAA
                     QAGLPTASLVFWSFVGSGALAGLAGFMFMARAGTISATAGAGLELESVAAAVVGGVSI
                     LGGSGTMVGAFLGAVFIDTLKLSLVRVPEVSEFWRDAFLGILILAAVILDTLLRNRFN
                     RRWSAEARKAMEATGPTAVGMARMAGGGGTADA"
BASE COUNT          430 a          668 c          640 g          267 t
ORIGIN      
        1 ggccgttgcg gatgttgaga acagtgaaca tggccaacac cactcccagc atcgagccgg
       61 agccgccgaa gatgctcacc cctccgagca ggaccatggt gatgatgtcg agctcgtagg
      121 tctcgccgag agtgccctga acagcgccga cccgggccgc gaacaggatc ccggcgagcc
      181 cggccaccgc tcccgacgag acgaagagcc gcaccttcac cgagtcgacc tcgatgccgg
      241 agaaccgggc gacatcggcg ctgttgccaa tcacatacac cttgcgcccg aacgcgctgc
      301 gctcgaggat gacccaggcc agggcaaggc cgacgaagaa caggacgaga gcgaacggga
      361 aaggcccgac gagagtctgc tggccgatgc tctcgaacca ctccgggaag tcccccaccg
      421 accggtcttc gagaaggaca cgggccgccc ctcgaaagcc gatcagcccc gccagggtca
      481 ccaccagcga gggcagcccg aatcgcgtga tgacgaggcc ctgtagaagc ccggcgatcg
      541 cgccgaccgc gatccctatc aggatggcga gagcgaacgg gagctctccg ccctcgtaca
      601 gagttgccat cactgccgcc gagaagccca tgaccgacgc cacggacagg tcgatctcgc
      661 cgttgattat gacgaacgtc atcacgacga cgacgatgat cttctctatc gacagccaga
      721 agagattcac gaagttcccg aaaccgagat agaacgggga cagggagacg ttgaccacga
      781 tcgtcgccgc caatacgacg atcaggatcg actcccaacg atgcttggcc gcccagtcac
      841 gcatcggcag tccccccgcc tcccgccatg cgggccatgc ccaccgccgt cggccccgtg
      901 gcctccatcg ccttcctcgc ctccgccgac cagcgacggt tgaagcggtt gcgcagcaag
      961 gtatccagga tgaccgcagc caggatcaag atcccgagga aggcgtcacg ccagaactca
     1021 ctcacctccg ggacgcggac caggctgagc ttgagggtgt ctatgaagac ggcgccgagg
     1081 aaggctccca ccatggtgcc ggatccaccg aggatgctga ccccgccgac gacagccgcg
     1141 gcgacggact cgagctcgag gccggcaccg gccgtggcgc tgatcgtgcc ggcccgggcc
     1201 atgaacatga atccggcgag tccggccagg gcaccggacc cgacgaacga ccagaagacg
     1261 agagacgccg tcggcaagcc ggcttgggcg gctgccgaag ggttggaacc gatggcgtag
     1321 atccgtctcc cccagcgcag tctcgccagg gcgaattgca gacccactac gacgaccacg
     1381 gcgatcacga acaccagccg cacttcgaat ccggcgatcg tgaacagggt gacgttgttg
     1441 aactcgagca cccaggaagg cagatcgccg gcgaggacgt tggccccctc cgagtagatg
     1501 ctgagcaggg tgcggaacaa ggccaatgtg cccagggtga cgatgatcgc cggcacgccg
     1561 ccgtaggcaa cgaggagacc gttgaccgat cccaggaccg ccccaaccac cgcggccacg
     1621 agcattgccg ggatcgggcc ttggccggtg gcggcgagga agtcgccggt gagataggcg
     1681 gcgacaccca cgatggagcc gaccgacaga tcgatgttgc ggctgaatat gaccagtgcc
     1741 tgggccgcgg cgagaagggt gacaaccacg acgctcgtgg tgacccggtt gaagaatcgc
     1801 ccactgagat agccctcgat gaggaacgcg aacaccacga tcgagaccgc gatgatcgcg
     1861 aggagagtcg cctctttcac ccgctccgga cgaaggaacc tggtgctcct gatgggtgcg
     1921 acggtggtgg actcggtcat ctggcgccca ccgcggcggt catgattcgc tcttgagttg
     1981 cctctcgacg cgggtagatg ccgat
//
LOCUS       DASTXO010000126         3036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1702180, whole genome shotgun sequence.
ACCESSION   DASTXO010000126 DASTXO010000000
VERSION     DASTXO010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1702180"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VFU96_02625"
     CDS             <1..659
                     /locus_tag="VFU96_02625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU5112182.1"
                     /translation="CIRLVWDPEQEPLGLDAVGVQGCPVRAPSRHRAQYVDAMWVADR
                     DFPQLDVTQSRVRLRLMGMITATGVMDNEQVYRSYAVELTRYATGLVGPFDAADVVTD
                     ACLKAFSARKWPEVSNKRAYLYRAVLSVANDHHRSTLTRRLREMKTASPESTPPQDID
                     FDVLGAVDRLSVQQRAAVLLTYWADLPVETVADRMGISTGAVKRHLARARKHLEEWLS
                     "
     gene            656..1786
                     /locus_tag="VFU96_02630"
     CDS             656..1786
                     /locus_tag="VFU96_02630"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HEU5112183.1"
                     /translation="MSRSDDRKMKELADRLVAMSPEPPPYPEEVSVTQREDSKPRPVL
                     VFAGAALVVLLLAAIPILLARGGGDVDPVASSTTSTVPTTVTTQPQASTTVTTEPPAT
                     TTTVPATTTTTVPAVEAVEAVLYAVQTPENSFTGNPALVGFRGTVVPGEEPMHPSYPG
                     LYALTSEDLFLPDGFSNMVPADVEILDVTSDEHTVTVDMNEAFLAGSGSGLLGDFTML
                     NQLIYTASAFGEIESVLFTVNGAPVTAFGSEGLDLSEPQGRDAYLDQLNSVNIDSPIQ
                     GQGDEPLRVAGFANVFEATVSLEVVDASGNVVQEDFTTATCGTGCWGAYEFEVDYPFV
                     GDETIRVFWHSAEDGSPSDVVSIPVLWDDPDGWDFSSHPGTP"
     gene            1875..2693
                     /locus_tag="VFU96_02635"
     CDS             1875..2693
                     /locus_tag="VFU96_02635"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5112184.1"
                     /translation="MPLDQYREANRANWDSRVPIHYGSDEYGIARFISDPTHLSGVVT
                     YDKEKLGDVSGKTLLHLQCHIGTDTISWARLGATVTGTDLSEKAIEAATRLSEDSGTP
                     ARFLVSELYDTPDVLNEKFDIVYTGVGAICWIPEIRGWAEVVSSFLEPGGVFYMREGH
                     PMMWAMDWLETEELRVAVAYFETAEPEGEEEHETYAGVGVVESPLNYGWNHGMGETLD
                     ALIRAGLRIERVEEYDECEWQGLPMMIQGEDGMWRLPDRRERLPLMWSVLATKL"
     gene            complement(2830..>3036)
                     /locus_tag="VFU96_02640"
     CDS             complement(2830..>3036)
                     /locus_tag="VFU96_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112185.1"
                     /translation="RGDLSTGPFEECRDGLPEWFDDNLDTHCLAVINGSVYAGHGGTV
                     WRSDDHGAQWTEVVSGLPRITCLA"
BASE COUNT          569 a          925 c          979 g          563 t
ORIGIN      
        1 ggtgcatccg cctcgtctgg gaccccgagc aggagcctct cggtctcgat gcggtaggtg
       61 ttcagggctg ccctgtccgg gccccttccc gtcatcgtgc tcagtatgtc gatgcaatgt
      121 gggtggccga tcgcgatttt ccgcaactgg atgtcaccca atcccgggtt cggctccgtc
      181 tcatgggcat gatcaccgcc acaggtgtga tggacaacga gcaggtatat cggtcatacg
      241 ccgtggagct caccaggtac gcgactggcc tggttgggcc gttcgacgct gcggatgtgg
      301 tgaccgatgc ctgtctcaag gccttttccg ccaggaaatg gcccgaggtg agcaacaaga
      361 gggcatacct ctatcgagcg gtgctcagtg tggccaatga ccaccacagg agcaccttga
      421 cgcggcggct gcgggagatg aagacggcat cgccggaatc gacgccgcct caggacatcg
      481 atttcgatgt cctgggtgct gtcgatcgtc tctcggtgca gcagagggcg gcagtgctcc
      541 tcacctattg ggctgatctc ccggtggaaa cagtcgccga tcgaatgggc atatcgaccg
      601 gagccgtgaa gcgtcatctg gcccgggcgc gaaagcacct cgaggagtgg ctgtcatgag
      661 cagatcagat gatcgaaaga tgaaggaact tgccgaccgg ctggtggcga tgtcgccaga
      721 gccaccgccc tatcccgagg aggtctctgt gacccaacgt gaagactcga aaccgcgccc
      781 ggtcctggtg ttcgcaggcg ccgcactcgt cgtgctgctc ctggccgcca tcccgatctt
      841 gctcgccaga ggaggcggtg acgtcgaccc cgttgcgtcg tcgacgacct cgacggtgcc
      901 gacaacggtc accactcagc cacaggcatc gacgaccgtc accacggagc caccggcgac
      961 gacaacgacg gtgccggcga ccacgaccac cacggttccc gcggtcgaag cggtggaagc
     1021 ggtgctctac gcggtgcaga cacccgagaa ctcgttcacg gggaatcccg ccctggtggg
     1081 cttccgcggg actgtcgtac cgggtgaaga gccgatgcat ccgtcctacc cgggcttgta
     1141 tgcattgaca tcggaggatc tcttcctacc cgatggattc agcaacatgg ttccggccga
     1201 cgtcgagatc ctggatgtga cttcagacga gcacaccgtc accgtggaca tgaacgaggc
     1261 cttcctcgcc ggatccggat cgggtctgtt gggggacttc accatgctca accagttgat
     1321 ctacacggcg tctgcgttcg gtgagatcga aagcgtcctg ttcacggtca acggcgcacc
     1381 agtcactgcc tttggcagcg agggcctcga tctgagcgag ccacagggcc gggatgcata
     1441 cctggaccag ctcaattcgg tcaatatcga ctcaccgatc caaggtcaag gcgacgagcc
     1501 attgagggtt gccggattcg cgaacgtgtt cgaagcgaca gtgagcctgg aggtggtcga
     1561 cgccagtggc aacgtggtcc aggaggactt caccaccgcg acatgcggaa cgggttgctg
     1621 gggcgcatac gagttcgagg tcgactaccc attcgtcggg gacgagacga tccgggtctt
     1681 ctggcattcg gcggaggatg ggtcacccag cgatgtggtc tcgatcccag tcctttggga
     1741 cgacccggac gggtgggact tctcctcgca cccgggtacc ccgtagggcc gttcctgatg
     1801 cagggcgtca cggattcgtg acgccctgca cttattccaa cctggtcccg acgacacgca
     1861 gccatactca gcccatgcca ctggatcaat accgagaggc gaatcgggcg aactgggact
     1921 cccgagtccc catccactac gggtcggacg agtacgggat cgccaggttc atctctgatc
     1981 ccacccacct gagcggagtg gtcacctatg acaaagagaa gctgggcgat gtgtcgggca
     2041 agacgctcct tcatctccag tgccacatcg ggaccgacac catctcctgg gcccggctcg
     2101 gggcaaccgt caccgggacc gacctgtcgg agaaggcgat cgaggccgca acccgtctca
     2161 gcgaggattc cggcacaccg gcccgcttcc tcgtctctga gctctacgac acgcctgacg
     2221 ttctcaacga gaagttcgac atcgtctaca ccggggttgg ggccatctgt tggatcccgg
     2281 agatacgggg ctgggccgag gtggtgtcgt cgtttctgga gccgggcggc gtcttctaca
     2341 tgcgtgaggg tcatcccatg atgtgggcga tggattggtt ggagaccgag gaactacggg
     2401 tcgccgtggc ctacttcgag accgccgagc ccgagggcga ggaagagcac gagacgtatg
     2461 ccggtgtcgg agtggtcgag agcccgctga actacggatg gaaccacggg atgggggaga
     2521 cactcgatgc cctgatccgg gctgggctgc gcatcgagcg ggtggaggaa tacgacgaat
     2581 gtgagtggca ggggctgccc atgatgatcc agggcgagga cggcatgtgg cgtttgccgg
     2641 accggaggga gcgcctgccg ctgatgtggt cggtccttgc gacgaagctc tgacaaatgg
     2701 gtcgtccgac ccccttcagc cgagcgacct acgtgccgaa ttattacggg taagtcatta
     2761 gtcacaagct aaggactttc ttcctcctcc agtgtgagag gggccgcttc ggtggcccct
     2821 ctttcgcgtt caggccaggc aggtgatccg aggaagcccg gaaacgacct cggtccattg
     2881 cgcgccgtgg tcgtccgacc tccaaaccgt gccgccatgg ccggcgtaca ccgacccgtt
     2941 gatgacggcg agacagtggg tgtcgaggtt gtcgtcgaac cactcgggca agccgtcccg
     3001 acactcctcg aacggtcccg tcgatagatc tccccg
//
LOCUS       DASTXO010000127         5318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1296672, whole genome shotgun sequence.
ACCESSION   DASTXO010000127 DASTXO010000000
VERSION     DASTXO010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5318
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296672"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1409)
                     /locus_tag="VFU96_02645"
     CDS             complement(<1..1409)
                     /locus_tag="VFU96_02645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5112186.1"
                     /translation="MILEVLRWVGYGTIAYFIFMQLYMIGLGLRSAAVMRRVHHLDRF
                     GRVSEMLSSQTSPPVSIVIPAYNEEAGIVDSVRSMSIVGYPRFEIVVTNDGSTDGTLA
                     ALIEAFHLERVRIPYRPDIATAPVRGIYRGRGTVDITVIDKENAGRADALNAGINAAR
                     YPYALCTDADVILDANCLIGAMRRVVEDRQRTIVVGGNIRPLNGSKVQLGHLVEAGVP
                     KRLVARMQVLEYLRTFLASRPAWSQMGALPLVSGAFGIWKRSAVIAVGGFTTGHMGED
                     MDLTMRLHRYHIDNEIPYRIVYEPSAVIWTEVPDTFRVLRRQRIRWHRGLMTAVRDFM
                     PMTFNPRYGKVGMVTWAAMFLFEYLAPIIEFLGWITIPLAFLLGALNTISLVILVTLA
                     FGVGVINSLVALLLDESYGYFNSLKDTLRLIVMILIENFGMRQVTVAWRIRAIVGGKR
                     TRVWGNMERKGVANLGVGRP"
     gene            complement(1406..2551)
                     /locus_tag="VFU96_02650"
     CDS             complement(1406..2551)
                     /locus_tag="VFU96_02650"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEU5112187.1"
                     /translation="MNETALGVFAVAVVFTLATVMLVGLVILKAVNRRRSLRRRNRRA
                     RYVALISRHLATNTLTEPIDGSVADDDAFLDAVIDIRNVVSGREIDTLAGIIDRVGLM
                     RRQEARLRSRFPLGRRLRAAVSLAEIGDESTAPVLLDHLSDREPEIRIQCARGLGRMQ
                     HEPGIEAILERFGLEAPWVRARFADTLVGFGQKATWPLVAHVRANLRHDHNEGVVEAI
                     RVLGIIGDREVGPSLAGILRVATDPEVRIAAIESLGLVGGPLAIRPLKYTFRSPDWRL
                     RAKSATSLGHIGDPSINRVLASGLQDSNWWVRRNSASALAGLPGGEDVLLQAIESGDP
                     FARDAAAEALADCGALAAARQRHDAGQATDSDRLLLDHVGVGVQVVS"
     gene            complement(2603..2971)
                     /locus_tag="VFU96_02655"
     CDS             complement(2603..2971)
                     /locus_tag="VFU96_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5112188.1"
                     /translation="MGEIKRVLVADDSDTVTTVLATALELEGYEVDTVDNGPAAYEMG
                     KAGDYDLVILDHLMPGLLGLEIMERWRAEGHTIATIVLSGVDDDRIAVDTLEVGAVDF
                     VRKPFRIPELMARIRSRLRD"
     gene            3127..3852
                     /gene="prmC"
                     /locus_tag="VFU96_02660"
     CDS             3127..3852
                     /gene="prmC"
                     /locus_tag="VFU96_02660"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEU5112189.1"
                     /translation="MTTIPEHERVRLEALARPDLPLDKLIARRLGGEPLQYIEGTAAF
                     GPLDLLVDERVLIPRPETEGMFELATRMVRSPEIIVDLCTGSGALALALKSQFPSAAV
                     FATDISPDAVEVATDNRYRTGLQVYLAEGDLFDPLPTAILGTVDLIVANPPYVAEVDF
                     DKLPADVQQEPRVALVSGPTGLEVIQRIGASAASWLRPGGVVICEIGEKQGVSATSSF
                     MDLPAVVRQDLTGRDRYIVAVKP"
     gene            3849..4409
                     /locus_tag="VFU96_02665"
     CDS             3849..4409
                     /locus_tag="VFU96_02665"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEU5112190.1"
                     /translation="MSLDRAIAAIRRREVVGLPTDTVYGIGADPLSEVAVARLFELKG
                     RPDHKPIGLLVASLEQAAVIGEIEGVAAELAAEHWPGALTLVVAPKVILADWVGDSQR
                     LTVGLRVPDHEIARELLEAVGPLAVTSANLSGDSESMSDEDARAVFGDLVPVYVEGRA
                     PGGEASTVVDVTGADVVVLRQGPVHV"
     gene            4456..>5318
                     /gene="glyA"
                     /locus_tag="VFU96_02670"
     CDS             4456..>5318
                     /gene="glyA"
                     /locus_tag="VFU96_02670"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU5112191.1"
                     /translation="MSMDPLEQVDPAIAGLIRKDVERQNTHIHLIASENFASPAVMAA
                     SGSVFTNKYAEGYPGRRYYEGCGVVDEMEQLAIDRAKELFGAEHANVQAHSGAQANMG
                     VYFSLLQPGDRVLGMRLDQGGHLTHGSPVNFSGLYYDFVAYGVDRETELVDMEQVRAL
                     AVEHKPKIVLAGYSAYSRTLDYEAFRVIADEIGAIFVVDAAHFIGLVAGRSHPSPVPH
                     ADIVTGTTHKALRGPRGGLILSTERFAKDVDRAIFPNAQGGPIESQIAAKAVSFHEAM
                     QPGFRSYTDQIV"
BASE COUNT          948 a         1703 c         1734 g          933 t
ORIGIN      
        1 gggcgtccca ctccgaggtt ggcgactccc ttgcgttcca tgttccccca gacgcgggtc
       61 cgcttgcccc cgacgatggc gcggatgcgc caggcgaccg tcacctgccg cataccgaaa
      121 ttctcgatca agatcatgac gatcagccgc aaggtgtcct tcagggaatt gaagtagccg
      181 tacgactcgt cgaggagcag ggctaccaga gaattgatca ctccgacgcc gaaggcgagg
      241 gtgaccagga tcacgagtga gatcgtgttg agggcgccga ggaggaacgc cagcgggatg
      301 gtgatccacc ccaggaactc gatgatcggg gccagatact cgaacaggaa catcgcagcc
      361 caggtgacca tccccacctt gccgtatctc ggattgaagg tcatcggcat gaagtcccgc
      421 accgcggtca tcaacccgcg atgccatcga atgcgctggc gtcggagaac ccggaacgtg
      481 tcgggaacct cggtccagat caccgccgat ggctcgtaga cgatccggta ggggatctcg
      541 ttgtcgatgt gatagcggtg aagacgcatg gtcaggtcca tgtcctcgcc catgtgaccg
      601 gtggtgaagc ctcccaccgc gatcacggcc gacctcttcc aaatgccgaa cgcgcccgag
      661 acgagtggca gagcgcccat ttgcgaccag gcgggtcggc tggcgaggaa ggtgcggaga
      721 tattccagga cttgcatgcg ggcaaccagc ctcttcggca cgccggcctc gaccagatga
      781 ccgagttgga ccttgctccc attcagtggc cggatgttcc cgcctacaac gatcgttctc
      841 tggcgatcct ccaccacccg tcgcatcgca ccgatcaggc agttggcatc gaggatgaca
      901 tcggcgtccg tgcacagcgc gtatggatat cgggccgcat ttatcccggc gttgagggca
      961 tctgccctcc ccgcgttctc cttgtcgatg accgtgatgt cgaccgtgcc gcgaccccgg
     1021 tagatgcccc tcaccggagc ggtcgcgatg tcggggcgat aggggatgcg gacgcgctcc
     1081 agatgaaagg cctcgatcag cgccgcgagc gtcccgtcgg tggaaccgtc gttcgtgacc
     1141 acgatctcga accgggggta gccgacgatc gacatcgacc ggaccgagtc cacgattcct
     1201 gcttcctcgt tgtacgcagg gatgacgatc gagactggag gcgaggtctg actggagagc
     1261 atctccgata cgcgcccgaa acgatccaga tgatgcaccc ggcgcatgac cgcagccgag
     1321 cggagcccga gcccgatcat gtagagctgc atgaagatga agtaggcgat cgtgccgtaa
     1381 cccacccagc gcaggacctc gaggatcacg acaccacctg gactcccacc ccgacgtgat
     1441 cgaggagcag tcggtcactg tcggtcgcct gtccggcgtc gtggcgttgg cgggccgcgg
     1501 ccaatgctcc gcaatcggcc agcgcctccg ccgcggcgtc ccgagcgaag gggtcgcccg
     1561 actcgatggc ttgcaacaac acgtcctcgc cccccggtag ccccgcaaga gcggaggcgc
     1621 tgtttcgccg tacccaccag ttggaatcct ggagccccga tgccagcacc cggttgatcg
     1681 aagggtctcc gatgtggccc agggacgtcg ccgacttggc ccggagcctc caatctgggg
     1741 aacgaaaggt gtacttcaac ggcctgatgg ccagcggccc gccgaccaga cccagcgact
     1801 cgatggcggc gatgcggacc tcggggtcgg tggcgaccct gaggatcccg gcgaggctcg
     1861 gcccgacctc cctgtctccg attatcccga gcacacgtat cgcttccacg acaccttcgt
     1921 tgtggtcgtg tcggaggttg gccctgacgt gagctaccag tggccaggtg gccttctggc
     1981 cgaacccgac gagtgtgtcg gcgaatcggg ccctgaccca gggggcctcc agtccgaacc
     2041 gctcgaggat cgcctcgatc ccgggctcgt gttgcatccg gccgaggcct cgggcgcact
     2101 ggattctgat ctcgggctcc cggtcactca gatgatcgag cagcacgggt gcggtcgact
     2161 cgtctccgat ctcggcgagc gacacggcgg cccgaagacg ccgccccaag gggaaccggc
     2221 tgcgcaggcg cgcctcttga cgtcgcatca acccgacccg gtcgatgatc ccggcgaggg
     2281 tgtcgatctc acggcctgag acgacgttgc gaatatcgat cacggcgtcg aggaacgcgt
     2341 cgtcgtcagc caccgagccg tcgatcggct cggtgagggt attcgtcgcc agatgacggc
     2401 tgatcagcgc cacatatcgg gcacggcgat tacgacggcg cagggagcgt cgtctgttca
     2461 ccgccttcaa aatgaccagt cccaccagca tgacggtggc cagggtgaag acgacggcca
     2521 cggcgaacac gccgagagcg gtttcgttca taggatcccg tgttattgat cggccccgta
     2581 gagggccggt tgagcattga ggtcagtccc gcaaccggga acggatgcgg gccatgagct
     2641 cagggatgcg gaatggcttt ctgacgaagt cgaccgcgcc cacctcgagg gtgtcgactg
     2701 cgattcgatc gtcgtcgact ccggacagca cgatggtcgc aatcgtgtgc ccttcggccc
     2761 tccaccgctc catgatctcc agcccgagca ggcctggcat gaggtggtcc aggatgacca
     2821 ggtcgtagtc tccggccttg cccatctcgt aggcggcggg accattgtcg accgtgtcga
     2881 cctcataccc ctctagttcg agggccgtcg ccaggaccgt ggtcacggtg tcggagtcgt
     2941 cggcaacgag gacgcgcttg atctcaccca tcagtggcct tcggtgtctt cctggagacg
     3001 gaattgcagg atgatgatgc cagaagggag cgctcctccc cggttgcgcg gcatcgaagt
     3061 gcccggcgac ggactcgatc cgaactatct tcacacggca gtcgtgatga gagattcctg
     3121 gggcgaatga cgacgattcc agaacacgag agggtgaggc tcgaagcgct ggcgcgtcct
     3181 gatttgccac tcgacaagct catcgcgcgt cgtctgggcg gcgagccctt gcagtacatc
     3241 gaagggacag cggcgtttgg gcctctcgac ctcctggtcg atgagcgagt cctcatcccc
     3301 agacccgaga ccgaaggcat gttcgagctg gcgacccgga tggtgcgcag cccggagatc
     3361 atcgtcgact tgtgcaccgg atccggtgcc ctagcgctgg cgttgaagag ccagttcccc
     3421 tcggcagcgg ttttcgccac cgacatctcg ccggatgcgg tcgaggtcgc gacggacaat
     3481 cgctaccgga ccgggctcca ggtgtacctg gcggagggcg acctcttcga cccactcccg
     3541 actgcgatcc tggggactgt cgatctcatc gttgcaaatc ccccctacgt cgctgaggtg
     3601 gacttcgaca agttgcccgc agacgtgcag caggaaccga gggtggcgct ggtctcaggc
     3661 ccaaccgggt tggaggtcat ccaaaggatc ggggcctcgg cggcctcatg gcttcgcccg
     3721 gggggagtgg tcatctgtga gatcggggag aagcaagggg tttcggcgac cagctcgttc
     3781 atggatctgc cggcggtcgt gcgccaagac ctcaccggac gcgatcgcta catcgtcgcc
     3841 gtcaagcctt gagcctcgat cgggcgatcg cagccatccg aaggcgggaa gtggtcggcc
     3901 tccccactga cacggtttat gggatcggag ccgacccgct gagcgaggta gcggtggcca
     3961 ggctgttcga gctcaagggc agacccgacc acaagccgat cgggctgctg gtcgcgtcgt
     4021 tggagcaggc ggcggtgatc ggtgagatcg agggcgtcgc tgcagaattg gccgccgagc
     4081 actggcccgg tgcactcacc ctggtcgttg cgccaaaggt gatccttgcg gattgggtcg
     4141 gcgactcgca gcggctgacg gttgggctgc gagtgcctga ccatgagatc gcccgcgagc
     4201 tgctcgaggc ggtcgggccg ctggctgtca cctcggccaa cctctccggc gacagcgagt
     4261 cgatgagcga cgaagatgcg cgcgcggtgt tcggggatct ggtgccggta tacgtggagg
     4321 ggcgtgcccc cggtggagaa gcctccaccg tggtggacgt aaccggggcc gatgttgtgg
     4381 tgctccggca gggcccggtg cacgtctgac tgccgcgact cgagctgatc cctgcccgtt
     4441 accctccgtc gcatgatctc gatggaccca ctggagcagg ttgatcccgc cattgccggc
     4501 ctcatccgca aagacgtcga acgacagaac acccacatac acctcatcgc atcggagaac
     4561 ttcgcctccc ccgccgtgat ggctgcctcg ggatcggtgt tcaccaacaa gtacgccgag
     4621 ggctacccgg gccgtcgcta ctacgaaggc tgtggggtgg tcgacgagat ggagcagttg
     4681 gcgatcgatc gggccaagga gctcttcggg gcggagcacg ccaatgtcca ggcccattcg
     4741 ggggcccagg ccaacatggg cgtctacttc tccctgctgc agcccggcga tcgagtgctg
     4801 gggatgcgtc tcgaccaggg tgggcatctg actcatggct caccagtcaa tttctccggc
     4861 ctctattacg acttcgtcgc atacggggtc gatcgcgaga ccgagctggt ggacatggaa
     4921 caggtccggg ccctggcagt cgagcacaag cccaagatcg tcctcgccgg ctactcggcc
     4981 tactcccgca ccctcgatta cgaggcgttc cgggtcatcg ccgacgagat cggtgcgatt
     5041 ttcgtggtcg acgcggcaca tttcatcggg ctggtggcgg ggcggtcgca cccgtcaccg
     5101 gtccctcacg ccgacatcgt gaccgggacc acgcacaagg ctctacgtgg tccccgaggg
     5161 gggttgatcc tgagcaccga gcgcttcgcc aaggacgtcg accgggcgat attccccaac
     5221 gcccagggcg ggcccatcga gtcccagatc gcggcgaagg cggtctcatt ccatgaggcg
     5281 atgcagcccg gcttccgttc ctacacagat cagatcgt
//
LOCUS       DASTXO010000128         3948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_854935, whole genome shotgun sequence.
ACCESSION   DASTXO010000128 DASTXO010000000
VERSION     DASTXO010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854935"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VFU96_02675"
     CDS             complement(<1..561)
                     /locus_tag="VFU96_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5112192.1"
                     /translation="MDHDHLDADLGAIGALTDPTRRRLYAFVARSIEPVGRDEAADAL
                     AIPRQTAAYHLDRLAEEGLVDVEFLRRSGRSGPGAGRPAKLYKRTQRDHEVSLPPRRY
                     GLAARILLEAVSLGAVKRKDLVAAAHRIGVGLAVQGLDQALRETGYDPVTEEGEIRFR
                     NCPFHALKEDDQKTVCNLNLGLVEGML"
     gene            646..1050
                     /locus_tag="VFU96_02680"
     CDS             646..1050
                     /locus_tag="VFU96_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014350975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112193.1"
                     /translation="MTTATSTETGASFVVHESRRAYQILHLGYTVAPILFGLDKFFNL
                     MTDWVDFLPAFVTDAVSGSVVMAIVGVIEIVAGIGVWFRPKIFAPIVAAWLALIIVTL
                     IVAGGFWDIALRDFGLLLGALALWRLALAHSN"
     gene            complement(1058..2626)
                     /locus_tag="VFU96_02685"
     CDS             complement(1058..2626)
                     /locus_tag="VFU96_02685"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU5112194.1"
                     /translation="MNERAGQVIMIVAAGFVLGLAVFFVTRGDDPTTTSVTGLSALPT
                     TTAAAITGSDPIQTTTTLPPTTTTTAPPQDREPGTVPGFTVGRPWGTTVGMTMFRGNP
                     TRTFYGQGPVPDSPTVAWRYPDEQMCSPSSEGGETRIWCGMGWTGQPAVWERPDGVTE
                     LIFGAYDSAVHFVDASTGQDLRPEFQTGDIIKGSVTLDPDGFPLLYFGSRDNQLRILA
                     LDREEPTVLWSMDANEVNGIWNDDWDSNPAIVDDIMYEGGENGWFFAIQLNRGYDSEG
                     KVVVDPVKLVQQPGYNEALLANSGRNVSIEASVLVFDERVYFSNSGGRIVGLDVSDVR
                     NGNAPIVFDYYAGGDIDATMVADAEGMIYVSIEHEPSQMSATEIERNREVGQLVKLNP
                     YSEGDPRVWGLDLTAGSSDAGSWSTPAVRNGVVYVNTHQGSLIAVDANDGTLLWEDEV
                     GFHSWSSPLIVDDTLVTATCLGDVRAYSLADPRTPAMNWSLSLGEACLEATPVVWNST
                     IYLGSRDGHMRALR"
     gene            complement(2639..3748)
                     /locus_tag="VFU96_02690"
     CDS             complement(2639..3748)
                     /locus_tag="VFU96_02690"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEU5112195.1"
                     /translation="MPRSLQLLIVLIGGALTGVAAFTIWGLTRDDTPPSSTVAQATEA
                     SSTATTSVTATSPTSTPPPTTTTTPTTTTTVPEKGYLVIQGTGDVAVDPEYIPTLVVE
                     GWDHAWSGLEGLFMEDDLTVVNLECVPSDLGSPLDKAFVFRCPTEALPSMKANGVDVA
                     NLGNNHSGDFGKEALVDGRDQLTAVGISPVGAGRNVAEAGQPAFFDINGWKVAVVGFG
                     GVAPDDTWYATADTPGMRSGDDTPSMVEAVAAAEAEADLVVVTIHWGMELDTTPRQDD
                     IERAQAMIEAGADVIFGHHSHRMQPLETVGNAAVFWSLGNFVWPHNSVPSATTAVARA
                     VVAPDGTIDSCLIPAYIETHGHPVLTGEAECAPPG"
     gene            complement(3793..>3948)
                     /gene="glnQ"
                     /locus_tag="VFU96_02695"
     CDS             complement(3793..>3948)
                     /gene="glnQ"
                     /locus_tag="VFU96_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432104.1"
                     /note="similar to ATP-binding component of ABC
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HEU5112196.1"
                     /translation="VVTHEMGFARAVANRVVFMADGEIVEVGSPEHFFTNPQEKRTKL
                     FLSQILE"
BASE COUNT          702 a         1248 c         1266 g          732 t
ORIGIN      
        1 gagcatcccc tccaccaggc ccagattgag gttgcacacc gttttctgat catcctcctt
       61 gagggcatgg aatgggcagt tgcggaatcg gatctctcct tcttcggtca ccgggtcata
      121 accggtctcg cggagagcct gatccagacc ctgcacggcc aacccgaccc cgatgcggtg
      181 ggcggccgcc accagatcct ttcgcttgac cgcgcccaat gagaccgcct cgaggagtat
      241 gcgagcggcg agcccgtacc gacgcggagg gagtgaaacc tcgtgatccc gctgggtcct
      301 cttgtagagc ttggccgggc gcccggcgcc gggccccgac cgaccgctgc gccgcaggaa
      361 ctcgacgtca acgagaccct cttcagcgag tcggtcgagg tgataggcgg cggtctggcg
      421 agggatggcg agtgcgtcgg ctgcctcgtc ccgtcctacc ggctcgatgg accgggcgac
      481 gaaggcatag agacgccggc gagtgggatc ggtcagagcc ccgatggcgc cgaggtcggc
      541 atcgagatgg tcgtgatcca tgccgtcact ctaaaacacg atttgtttga cactagattt
      601 tcgagctgta aaaatgacta tgttcattct tagaaggagg agatcgtgac aaccgctacc
      661 agcaccgaga ccggcgcttc attcgtggtc catgagtcca ggcgcgccta ccagatcctt
      721 catctcggct acaccgtcgc cccgatcctc ttcgggctcg acaagttctt caatttgatg
      781 acggactggg tcgatttcct gcctgcattc gtcaccgacg cagtcagcgg atctgttgtc
      841 atggcgatcg tcggcgtgat cgagatcgtc gcggggattg gagtctggtt ccggcccaag
      901 atcttcgctc ccatcgttgc ggcatggctc gcactgatca tcgtcaccct gatcgtggcc
      961 ggtgggttct gggacatcgc cctgcgagac ttcggcctgc ttctcggcgc cctcgccctg
     1021 tggcggctcg ccctcgccca cagcaactag cggccaatca cctgagcgcg cgcatgtggc
     1081 cgtcccgcga gccgagatag atggtgctgt tccagacgac cggcgtcgcc tcgagacagg
     1141 cctcacccag actcaatgac cagttcatgg ccggggtgcg ggggtcggcc agggagtagg
     1201 cccgcacatc gccgagacag gtggccgtga cgagagtgtc atcgacgatg agcggcgagg
     1261 accacgagtg gaacccgacc tcgtcctccc agagcagtgt cccgtcgttg gcatcgaccg
     1321 cgatcagtga cccttgatgg gtgttgacat acaccactcc gttgcgaact gccggcgtcg
     1381 accaggatcc ggcgtccgaa gacccggcag tcaggtcgag tccccagact ctggggtcac
     1441 cctcggaata tgggttgagc tttaccagct ggccgacttc gcgattccgt tcgatctccg
     1501 tggcactcat ctgtgagggc tcgtgctcga tcgagacata gatcattccc tcggcatcgg
     1561 cgaccatcgt ggcgtcgatg tcaccgccgg cgtagtagtc gaacacgatt ggagcattcc
     1621 cattacggac atcggacaca tccaatccca cgatccggcc gccggagttg gagaagtaga
     1681 ccctctcgtc gaagaccagg accgaggcct cgatggagac gttgcgcccc gagttggcga
     1741 gtagcgcctc gttgtaaccg ggctgctgga ccagcttgac cgggtcgacc accactttgc
     1801 cctcggagtc gtagccccga ttgagctgga tggcgaaaaa ccagccgttc tccccaccct
     1861 catacatgat gtcgtcgacg atcgctgggt tcgagtccca gtcgtcgttc cagatcccgt
     1921 tcacctcgtt tgcgtccatc gaccagagca ccgtcggctc ctcgcggtcg agggcgagga
     1981 tcctcagttg gttgtccctc gaaccgaagt agagcagcgg gaatccgtcg gggtcgaggg
     2041 tgacggaccc tttgatgatg tccccggtct ggaactcggg acgcaagtcc tgtccggtgg
     2101 aggcatcgac gaaatggacc gccgagtcgt aggcgccgaa gatcagctcg gtcacgccat
     2161 cgggacgctc ccacaccgca ggctggccgg tccagcccat accgcaccag atccgggtct
     2221 caccaccttc tgaggatggg ctgcacatct gctcgtctgg atagcgccag gcgacggtcg
     2281 ggctgtcggg cacagggccc tgcccataga aggtgcgggt ggggtttccc cggaacatcg
     2341 tcattcccac tgtcgtcccc cacgggcggc cgacagtgaa cccgggcacg gtccctggct
     2401 cccgatcctg aggaggtgcc gtggtggtgg tcgttggagg cagggtggtg gtcgtctgga
     2461 ttgggtcgga gccggtgatg gccgcagccg tggtggtcgg gagtgcagat agacccgtca
     2521 ccgaggtggt cgtcgggtcg tcgccgcggg tcacgaagaa cacggcgaga ccgagcacga
     2581 atccggcggc gacgatcatg atcacctggc cggctctctc gttcacgaag ccgtcctcct
     2641 aacccggcgg agcgcattcg gcctcaccgg tgaggacagg atggccatgt gtctcgatgt
     2701 atgcagggat caaacacgaa tcgatggtcc cgtcaggggc caccacggcc ctggccacgg
     2761 cggtggtggc gctggggacg gagttgtgcg gccacacgaa attgccgagg gaccaaaaga
     2821 cggcggcgtt gccgaccgtc tccagcggct gcatgcgatg ggagtgatga ccgaagatca
     2881 cgtcggcccc tgcctcgatc atcgcctgag cccgctcgat gtcgtcctga cggggagtcg
     2941 tatcgagctc catcccccaa tggatggtga ccaccacgag atctgcctct gcctcggcgg
     3001 cggccaccgc ctcgaccatg gagggcgtgt cgtcaccgct ccgcatcccc ggcgtgtcgg
     3061 cggttgcgta ccaggtgtcg tcgggggcga caccaccgaa cccgaccact gccaccttcc
     3121 acccgttgat gtcgaagaag gccggctgac ccgcctctgc cacgtttcgg cccgcaccca
     3181 ccgggctgat cccgacggct gtcaactgat cgcgaccgtc gaccagagcc tccttgccga
     3241 aatcacccga gtggttgttg cccaggttgg ctacatcgac gccgttcgcc ttcatcgagg
     3301 gaagcgcctc ggtcgggcag cggaacacga aagccttatc tagaggagaa cccaggtcgg
     3361 aggggacgca ctccagattg acgacggtca ggtcatcctc catgaagagc ccttccagac
     3421 ctgaccaggc gtgatcccag ccctcgacga caagagtggg gatgtactcg ggatccacgg
     3481 ccacgtcccc ggtgccctgg atcaccagat agcccttctc gggaacggtg gtcgtcgtgg
     3541 tcggtgtggt cgtggtagtg gggggcggag tcgaagtagg cgacgtcgcg gtgacactcg
     3601 tcgtcgcggt cgatgatgct tccgtcgcct gggcgaccgt cgacgaggga ggtgtgtcat
     3661 ccctggtcag accccaaatg gtgaaggcgg cgacgccggt aagggcgcca ccgatcaaga
     3721 cgatgaggag ctggagagac ctgggcactg cgtcaaggct aagagcctcg tggccgctat
     3781 cggcgctacc tgctactcga gaatctgact caggaagagc ttggtgcgct tctcctgtgg
     3841 gttggtgaag aagtgctcgg gggaaccgac ctcgacgatc tccccgtctg ccatgaacac
     3901 cacccgattg gccacggctc gggcgaagcc catctcgtgg gtgacgac
//
LOCUS       DASTXO010000129         2503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1259920, whole genome shotgun sequence.
ACCESSION   DASTXO010000129 DASTXO010000000
VERSION     DASTXO010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259920"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VFU96_02700"
     CDS             <1..463
                     /locus_tag="VFU96_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5112197.1"
                     /translation="TATTLAEVLGTNTGATSYHLRKLASVGLVEETGEGKGRERWWRP
                     THEMHSWFTSDIDEDPDGIAAAEWLHGESLRRFQVFAEEWFASEWDWPVEWRDAAGSS
                     DYLLELSARQLRDLGKELTTVIDRYRKLEPGPDPARVIFCTFAFPQPDGRI"
     gene            460..1716
                     /locus_tag="VFU96_02705"
     CDS             460..1716
                     /locus_tag="VFU96_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018785052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5112198.1"
                     /translation="MTEALTAEAVRRRYLTLVAMRWFPTGLLIPVSILLPAERGLSLS
                     QIGLAAAGQGLVVLFLELPTGGLADSLGRKRVLITSSFVGMASVAIYLAADSFGAFFA
                     AFALQGVFRALDSGPLEAWYVDATHASDPDAEIQRGLSGAGIALGVAIAAGALLSGLL
                     VTLDPIRGLEPLATPVVVSLGLQVLSAAGVAILMTEIRADKGWQAATRAASETPAAIR
                     GGLRLLRDNRVLLAIVSVELFWGFGMITFESFIPLRLAEIMGNAERAAAITGPAASAA
                     WLISAAGAATVPWLGRSLGLAPAAALLRILQGLAVVGIGFAAEAPGVIAAYLAAYLIH
                     GASNPIHMTLLHRQVDGTFRATVSSLNSMAGLSAAAFGLIALTALAEATSLSTAAYVG
                     AFVLAAAAPLYIPAWRQSRKKEPARV"
     gene            complement(1725..>2503)
                     /locus_tag="VFU96_02710"
     CDS             complement(1725..>2503)
                     /locus_tag="VFU96_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015242132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEU5112199.1"
                     /translation="DFVVLGIQRGGENLDREPTVLAVGDTMLLQGTWDALDRRVESDR
                     DVLVVDSPSLIRRQAIALGPRAWWAILILFGMVVLLATNAVPAVEAALLAAGAMVLSR
                     VVTIYQAYRSISWTTVVLVAGMIPLSTAVTQSGAADYIAQLLIDVVGDLGPYALLLGL
                     FVLTAIFGQLISNMATALIVIPIAVTAAIDMGISVRPVLMSLTVAAAGAFLTPVATPV
                     NLMVQGPGGYEFGDYWKLGLPMLLLFMVAAVLLVPVFWPF"
BASE COUNT          416 a          842 c          831 g          414 t
ORIGIN      
        1 aacggccacg accctcgccg aggtcttggg caccaacact ggagcaacga gctatcacct
       61 gcggaagctg gcttcggtcg ggctggtcga ggaaaccggt gaggggaagg gtcgggagcg
      121 ttggtggcgg cccacgcacg agatgcattc ctggttcacc agcgacatcg atgaggaccc
      181 cgatgggatc gccgccgcgg aatggctgca tggtgagtcg ctgcgccgat tccaggtctt
      241 tgccgaggaa tggttcgcat cggagtggga ctggccagtc gagtggcggg atgctgcggg
      301 ttcctccgac tacctcctgg agctctctgc ccgccaactc cgcgatctcg gcaaggaatt
      361 gaccacggtg atcgatcgct accgcaagct cgaaccaggg cccgacccgg cccgggtcat
      421 cttctgcaca ttcgccttcc cccagccgga cgggcggata tgaccgaggc cctcaccgcc
      481 gaggcggtcc gtcggcgcta cctgaccttg gtcgcgatgc gatggtttcc cacagggctt
      541 ctgataccag tctccatcct gctgccggcc gaacggggcc tttcattgag ccagatcgga
      601 ctggcggccg ccgggcaggg tctggtcgtc ctctttctcg agcttccaac cggagggctg
      661 gccgactcgc tgggccggaa acgggtgctg atcacgtcct ccttcgtcgg gatggcgtcg
      721 gtcgccatct acctggcagc cgattccttc ggggccttct ttgcggcgtt tgccctgcaa
      781 ggggtgttcc gggctctcga cagcggccca ctggaagcct ggtatgtcga tgcaacccac
      841 gcctccgatc ccgacgcaga gatccaacga gggctcagtg gcgccgggat cgccctcggc
      901 gtggcaatcg ccgccggtgc cctgctcagc gggctgctgg tgaccctgga cccgatcagg
      961 ggtctcgagc cgctggcgac tccggttgtc gtctccctgg gcttgcaggt gctcagcgcc
     1021 gccggtgtgg ccatcctgat gaccgagatc cgcgccgaca agggatggca ggctgcgacg
     1081 cgtgccgcca gcgagacccc ggccgcgatc agaggaggcc tccggctgct ccgagacaac
     1141 cgcgtcctcc tcgccatcgt ctcggtcgag ttgttctggg ggttcgggat gatcacattc
     1201 gagagcttca tcccgttgcg tctcgccgag atcatgggta acgctgaacg ggcggccgcg
     1261 atcaccgggc ccgccgcatc cgcagcttgg ctgatctcgg cagctggagc ggcaacggtg
     1321 ccctggctgg gccgtagtct cggcctggca cccgccgcgg cgttgctgcg gattctccag
     1381 ggactagccg tggtcggcat cggcttcgcc gccgaggcgc ccggagtgat tgcggcctat
     1441 ctggccgcct acctgatcca tggcgcctcg aacccgatcc acatgaccct gctccatcgg
     1501 caggtcgacg ggacgtttcg ggccacggtg tcctccctca actcgatggc gggcttgtcc
     1561 gctgccgcgt tcggactgat cgccctcacc gccctcgccg aggcgacttc gctgagcacc
     1621 gcggcctatg tcggcgcgtt cgtcctcgcc gccgccgccc cgctctacat ccccgcgtgg
     1681 cggcagtcac gtaagaagga gccggccagg gtctgaccga cccatcagaa cggccagaac
     1741 accggaacca gcaggaccgc cgccaccatg aaaagcagca gcatggggag cccgagcttc
     1801 cagtagtcac cgaattcgta cccgcctggg ccctggacca tgaggttgac cggcgtggca
     1861 accggggtga gaaaggcacc ggcagccgcg acagtcagcg acatcagcac cggccgcacc
     1921 gagatcccca tgtcgatggc cgcggtgacc gcgatcggga tcacgatcaa ggccgtggcc
     1981 atgttggaga tgagctggcc gaagatggcg gtgagcacga acaagcccag gagaagtgca
     2041 tagggcccga ggtcgccgac tacgtcgatg agcagctggg cgatgtagtc ggcagcaccg
     2101 ctctgggtga ctgcagtcga gagcgggatc atgccggcta ccagcacgac ggtggtccag
     2161 gagatcgacc gataggcctg atagatggtg accacccgcg acaggaccat cgctcccgcc
     2221 gccagcaggg ccgcctcgac cgcgggcacc gcgttggtcg ccaggaggac gaccatgcca
     2281 aacaggatca ggatggccca ccaggcccgc gggccgaggg cgattgcctg acgacggatc
     2341 agcgacgggg agtcgaccac gaggacatcc cggtccgact cgacccttcg gtccagggca
     2401 tcccaggtcc cctgaagaag catggtgtca ccgacggcga gaaccgttgg ctcccgatcc
     2461 aggttctcgc cgccgcgctg gatcccgagg acgacgaagt ctc
//
LOCUS       DASTXO010000130         2477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_449542, whole genome shotgun sequence.
ACCESSION   DASTXO010000130 DASTXO010000000
VERSION     DASTXO010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449542"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VFU96_02715"
     CDS             complement(<1..264)
                     /locus_tag="VFU96_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017487828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEU5112200.1"
                     /translation="MQVPRQGQGPCRCSTRSRIGVLGMAQDQQTQFDERVIQINRVAK
                     VVKGGRRFSFTALVVVGDGAGKVGIGYGKAKEVPAAIQKGMETA"
     gene            complement(198..551)
                     /gene="rplR"
                     /locus_tag="VFU96_02720"
     CDS             complement(198..551)
                     /gene="rplR"
                     /locus_tag="VFU96_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU5112201.1"
                     /translation="MRGSREDARSRRHTRVRKRVHGTGERPRLAVYRSNKYIYAQVID
                     DVEGRTLAAASSQESDLRSDNLNLAAAAKVGEAIASRAKDAGVKTVVFDRGGYKFHGK
                     VKALADAAREAGLEF"
     gene            complement(551..1087)
                     /gene="rplF"
                     /locus_tag="VFU96_02725"
     CDS             complement(551..1087)
                     /gene="rplF"
                     /locus_tag="VFU96_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU5112202.1"
                     /translation="MSRIGKMPVDMPSGVDIKVDGTQVTVKGSKGELSRQFTDRVSFS
                     VEEGVITVGREDDTRESRALHGLSRALLANMVHGVSEGFTKVLEIQGVGYRASLKGSD
                     LELLVGFSHAVDVKAPKGITFEVPEPTRIVVSGIDKEQVGQVAADIRKVRPPEPYKGK
                     GIRYAGEYVRRKAGKAGK"
     gene            complement(1089..1484)
                     /gene="rpsH"
                     /locus_tag="VFU96_02730"
     CDS             complement(1089..1484)
                     /gene="rpsH"
                     /locus_tag="VFU96_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU5112203.1"
                     /translation="MVTDPIADMLTRIRNAIAVGHERVVMPSSKMKVGIARILLDEGF
                     IDRYDVKADGALSELELVLRYGNRRQPAIEGIKRVSKPGHRVYRGASELPRVQGGLGV
                     AVVSTSQGLLPDREARRRRLGGEVVCEVW"
     gene            complement(1484..1669)
                     /locus_tag="VFU96_02735"
     CDS             complement(1484..1669)
                     /locus_tag="VFU96_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008633399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEU5112204.1"
                     /translation="MAKKSLVVKASRTPKYKVRGYSRCQRCGRARAYLRDFGMCRICV
                     RELASRGELPGVRKASW"
     gene            complement(1683..2246)
                     /gene="rplE"
                     /locus_tag="VFU96_02740"
     CDS             complement(1683..2246)
                     /gene="rplE"
                     /locus_tag="VFU96_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009141466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU5112205.1"
                     /translation="MSEQPRLKVLYAEQLAPRLKDELGLANVMEIPRLEKIVVNMGVG
                     EAVQDKKSIDSAMDELTLITGQKPKLNRSRKSIANFKVREGMPVGASVTMRGTRMWEF
                     FDRLLGIAIPRIRDFRGLNPRSFDGRGNYSFGVTEQLIFPEVDFDQVTSTRGMDITIC
                     TTATNDEQAKALLDAFGFPFRKATTGV"
     gene            complement(2243..>2477)
                     /gene="rplX"
                     /locus_tag="VFU96_02745"
     CDS             complement(2243..>2477)
                     /gene="rplX"
                     /locus_tag="VFU96_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU5112206.1"
                     /translation="RVISDKGKVLVEGVNTAKRHRRPTGSTMQGGIIDKDMPIDASNV
                     MIVCEKCGPTRIGHRIDDKGKKHRTCVKCGGDL"
BASE COUNT          412 a          839 c          731 g          495 t
ORIGIN      
        1 tgccgtctcc atgcccttct ggatcgctgc cggcacttcc ttggccttgc cgtagccgat
       61 cccaaccttg cccgccccgt cgcccaccac cacgagggca gtgaagctga accgccgacc
      121 acccttgacc actttggcca cgcggttgat ctgaatcaca cgctcgtcga actgggtctg
      181 ttgatcttgg gccatgccta aaactccaat ccggcttcgc gtgctgcatc ggcaagggcc
      241 ttgaccttgc cgtggaactt gtatccgccc cggtcgaaga cgaccgtctt cacccccgca
      301 tccttggccc ggctcgcgat cgcctcgccc accttggccg cggcggccag gttgaggttg
      361 tccgagcgca ggtccgactc ctgtgaggaa gcggcagcca aagtgcgtcc ctcgacatcg
      421 tcgatgacct gggcgtagat gtatttattg ctccggtaga cggcgagacg agggcgctcc
      481 cccgtgccgt ggacgcgctt gcgcacccgg gtgtgacgcc ggctccgggc gtcttctcgt
      541 gagcctcgca tcacttacct gccttcccgg ccttgcgccg aacgtattcg ccggcgtacc
      601 tgatcccctt gcccttgtag ggctcgggcg gacgcacctt gcggatgtcc gccgccacct
      661 ggccgacctg ctctttgtcg atccccgaca ccacgattcg ggtcggctcg ggcacttcga
      721 acgtgattcc cttgggcgct ttcacatcga ccgcgtggct gaacccgacc agcaattcga
      781 gatccgagcc cttgaggctg gcgcggtagc caacgccctg gatctcgaga accttggtga
      841 acccttccga gacaccgtgg accatgttgg cgagaagggc gcggctgaga ccatgcaagg
      901 ccctcgactc ccgggtgtca tcttcccgac ccaccgtgat cacaccttct tccaccgaga
      961 agctgacccg gtcggtgaat tgacgcgaca actcgccctt ggatcccttg acggtgacct
     1021 gggtcccgtc aactttgatg tcgacaccgg acggcatgtc caccggcatc ttcccgatac
     1081 ggctcatctc accagacctc gcacacgacc tcgccgccaa gacgacggcg ccgggcctcc
     1141 cgatcaggga gcagaccctg gctggtggag accactgcga ctccaaggcc tccttggaca
     1201 cggggcagct ccgacgcccc gcgatacacg cggtgacccg gcttggacac ccgcttgatg
     1261 ccctcgatgg cgggctgacg gcggttgccg tagcgcagca ccagctcgag ctcgctcagg
     1321 gcaccgtcgg ccttgacgtc gtaacggtcg atgaagccct cgtccaagag gatgcgggcg
     1381 atgccgacct tcatcttgga ggaaggcatc acgactctct cgtgcccgac cgcaatcgca
     1441 ttgcggattc gggtgagcat gtcggcaatc ggatcggtga ccattaccag gaagcctttc
     1501 tcacgccggg gagctcgcca cgtgacgcca gctcacgcac gcagatgcgg cacatgccga
     1561 aatcacgcag gtaggcgcgg gcgcggccac aacgctggca gcggctgtac ccgcggacct
     1621 tgtacttcgg cgtgcggctg gccttgacga ccagcgattt cttagccatt cagaatcctc
     1681 cttcagaccc cggtggttgc cttgcggaat gggaatccga aggcatcgag caacgccttg
     1741 gcttgctcgt cgttggtcgc tgtcgtgcaa atcgttatgt ccatgcccct ggtcgacgtc
     1801 acctggtcga agtcgacctc tgggaatatc agctgctcgg tcaccccaaa gctgtagttg
     1861 ccccgaccgt cgaaagacct cgggttgagc ccgcggaagt cacggatgcg agggatggcg
     1921 atcccgagca gacgatcgaa gaactcccac atgcgtgtcc cgcgcatcgt caccgaggcg
     1981 ccaaccggca tgccctcgcg caccttgaag ttggcgatgg acttccggga acggttcagc
     2041 ttcggcttct gcccggtgat gagtgtcagc tcgtccatgg cggagtcgat cgacttcttg
     2101 tcctggaccg cttcgcctac acccatgttg acgacgatct tctcgagacg cgggatctcc
     2161 atgacgttgg ccagccccag ctcgtccttg agacgggggg cgagctgctc ggcgtagagc
     2221 accttcaggc gaggttgctc gctcataggt cacctccaca cttgacacat gtccggtgct
     2281 tcttgccctt gtcgtcgatg cgatgcccga tgcgggtcgg gccgcacttc tcgcagacga
     2341 tcatcacgtt cgaggcgtcg atgggcatgt ccttgtcgat gatcccgccc tgcatggtcg
     2401 agcccgtggg gcgacgatga cgttttgcgg tgttgactcc ttcgaccagg accttgccct
     2461 tgtccgagat gacacgg
//
LOCUS       DASTXO010000131         8665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1002202, whole genome shotgun sequence.
ACCESSION   DASTXO010000131 DASTXO010000000
VERSION     DASTXO010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8665
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002202"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..2398)
                     /locus_tag="VFU96_02750"
     CDS             complement(128..2398)
                     /locus_tag="VFU96_02750"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEU5112207.1"
                     /translation="MDDIPYFEPLSHLRLEDRRLVTGSGRYVADLAPAGTVHLAFVRS
                     TEAHARITSIDTSVDSPGVVGAFTAADLALADIPGDSTVVPAPDFPRPHLASQKVRYV
                     GEPIAVVAADTAAQAVDAADLIWVDYELLPAVVDPRASLEDEVTLHESAGTNVVSRSE
                     LSAGVPKDVYEVVAEVEVINQRLAPNPIEPLAILVIPEDDGRLTVYVSHQRPHGVRAR
                     LCQLLPHDPERMRVVVPDVGGAFGMKGMTYPEHTVTAALALRLGRPVMWAERRREHLS
                     GGTHGRGSVHRVRLMGTSEGRIERAEIDVVADVGAYPHNGATIPMFSRLVALGLYDIA
                     SVSLTTTSVVTNTAPTGSYRGAGRPEAAYAIERAVDAFARAAGVDPVEVRLTNFIPSD
                     SLPYRTATGALYDSGDYAAALQRAVELVDLDKLREDQRGRLTEGRDPIGVGFGAFVER
                     AGGAVDAGEYGRVEIDREGKATLRTGSASSGQGHETAWAQVAAAALGLTPDDVTYLAG
                     DTDEVARGTGTFASRSAQLGAAAIWRTAHAVRGRAIELTAELLEASPADVVLADGAFS
                     IAGVPGTEMGWPEVAAAAHDRGIDLSEEEWFVPGAQTFPYGVHVAVVEVALETGQVRL
                     ERLVTVDDCGNVINPMIVEGQLHGSLMQGVGQALFEGVQYSEQGQLLTSTLMDYALPR
                     AGDAPPITSERLVHPAPSNPLGVKGTGEAGCIGAPPAIVNAVLDALAPYGVTDLQMPL
                     RPATVWAALSASNLLP"
     gene            2468..3460
                     /locus_tag="VFU96_02755"
     CDS             2468..3460
                     /locus_tag="VFU96_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HEU5112208.1"
                     /translation="MDSRRPKVAILGTRFGDFDVERGVLGDVDLVSGPGQTSQDVIEI
                     AGGADVILAGAAPRFDAATLEKLDCRAIVRLGVGVDGIDLDRARSLGMWVINVPDYGM
                     DAVALHAVTLVLASLRRLTTADRKLREGSWGVTDLRPLRLPSSLTVGVMGFGRIGRRV
                     AVLLAALGFGTVLISDPAVEASDVTTMLGETPARLVGVDDLLAGSDVVTLHSPPPDTG
                     YLLGATELGRMKEGSVLVNTARGALVDTGALVAALDGNRPGYAALDVFEQEPPDVSIF
                     EPVADRLILTPHMSWYTEESELALRRQAAAEARRILNSEEPVHAVVTPEAAEIR"
     gene            3463..4224
                     /locus_tag="VFU96_02760"
     CDS             3463..4224
                     /locus_tag="VFU96_02760"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEU5112209.1"
                     /translation="MAGRSINDLASPDVADMIGRTGLVVMPFGSVEQHGPHLPCGTDT
                     MAADLVAAELAERLDAILVPAGPYGVTPIHAGHPGTISLRRDVFEAYLANLCQEMVAM
                     GARTFVFVNWHEGNIASLDAVATELQDREDVLVVTAHACYTAQRVYSPAGGELTHGGG
                     IETLAVLAHDPDLFHADRVYEPHRPPGADELDTMRRSREVYGYITDVTEIADDGWYGN
                     PEWADQARAGQFAAVVSDEIAVAVESILEMRRRGT"
     gene            4221..4610
                     /locus_tag="VFU96_02765"
     CDS             4221..4610
                     /locus_tag="VFU96_02765"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5112210.1"
                     /translation="MTVVGPGELEFRDFPGRTTADPFRAAGQGASTVRQVIIEHVPSR
                     SPHRHPHSEEIVYVVAGRGRLWVDGVVHSVGPGSWYRIPIGTPHATMADPNERMSLVC
                     FFPHDDFASNIEELDVVLEVDGEANDE"
     gene            4603..5661
                     /locus_tag="VFU96_02770"
     CDS             4603..5661
                     /locus_tag="VFU96_02770"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5112211.1"
                     /translation="MSERVRLASIGLGRWARVLARGAQRGEVIELVSCFSRSPESRLE
                     FQTEFGIPRAAPSLDELLSDPEIEGVLVTTPNDTHKNVILESLDAGKAVYTDKPIAHT
                     MEDAGQIARAAAASGRPFAVGHSARRLGGHRAMKQWVETGKIGKVSLAEANFSNERGL
                     ELTTDTWRWFADKSPGGAMIQLGVHHADNLQYLLGPVAAVSSHARRLYTKAEVPDAIM
                     SILEFESGPLGYLGTGWASPGVYQLRLQGTEANLFYDLDFNNWDESHLADSKSTLVSQ
                     AYRESTRPNVDIPHTDMFREQLDEFALAIRGEAGIEVGALEAIRALAVVHAALESSNR
                     NGEAVAVAPMVERAGALE"
     gene            5658..6761
                     /locus_tag="VFU96_02775"
     CDS             5658..6761
                     /locus_tag="VFU96_02775"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5112212.1"
                     /translation="MRVDPATTRTIHHPDAVIQEVLDIWAARFLHGQIALGDLTATVE
                     RISTWSDWGPEWMKTARVHEELGEQAWEEGRRISAVAAFLNAAACYHLSYFLSVDDEK
                     AHAHGLAKMVECHDRVLPYMQPQVEKVRIPFPDADLVGLLSVPSVGGPSPVVIFLPGL
                     DSTKEQRHGGRGALLRRGMAVLSLDGPGQGETGAKLPIRHDYEVAVSAAIDYLERRYE
                     VDATRVGLIGASLGGYYACRAAAFEPRVTAAVANCGPYSWIECWDELPEVTRGAFRKY
                     SWSASDEEAREKAKALDLTGVAEEITQPLVVVHGMLDPLIGVEHASRIADEAPNAILI
                     TVDDATHGVSNLGYKFAPWVDDWMAGQLGGAIT"
     gene            complement(6769..8043)
                     /locus_tag="VFU96_02780"
     CDS             complement(6769..8043)
                     /locus_tag="VFU96_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEU5112213.1"
                     /translation="MTPGDVDFKDAVGKMHTEVFGRVANTPWYSDAVYERFSDAEYQR
                     RFTATQEKMKRLGLDVLIASGGPNHWSWGGGMRWLSNHWEWHGMSAYAVVPPEGEPVL
                     ICGPGGAHREAVRRATPLKDVRHSRWGHSGEVIVDLIEEWGLADATIGISCVDPVYGH
                     HLPVDEWERLRDGLPNATLERVGDFFHELVHVKSPEEIECVRKAGQLMDQAFHAMIAI
                     ARPGVTEYQLAAAATHAVMDGGGQTNFNIIGSTRMDDPGMVFGNPWPSSRRLEKGDII
                     INELACGYNGYSVQLGTPFCIGEPPSWIREFFDEVVVPGFELMAEQLVGGKSWDDVRK
                     AGDFFRDKGYDSRSLYLHCIDFVSHPPHVMWDHVVADETELELAPGTVAMLEPTIITP
                     DGLFGIFFGRTYVLTDGGHEKVTKFPHELIVI"
     gene            complement(8055..>8665)
                     /locus_tag="VFU96_02785"
     CDS             complement(8055..>8665)
                     /locus_tag="VFU96_02785"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU5112214.1"
                     /translation="SDDIVRDWYRRLTAESSVPFFLFNTSYSGYSLSPQLIEELSDLE
                     TVCGIKNPKPMDHLLQVQDLVGDRIVVCDADEGDWLGLHLEHGFRSLMSTPALAMYQT
                     PAHRPIVDYTRLADAGDTEGAWALQGTLVRERSAFEKWMRDQWLAQGAGAIPIAELKA
                     WLGLMGLEQGPVRPPLIPLGDERMEELRSDLDGLGLLGSVAS"
BASE COUNT         1505 a         2803 c         2835 g         1522 t
ORIGIN      
        1 gcgtacaggc cgtcccaagt ctcgacgtac ttgagctcga cggggatgtc ggagccggac
       61 acttcctcga cgatcgtcac gttctctgga tccacagcca tgactgccct ttcctcctgc
      121 acaatcgtta gggcaggaga ttagatgccg acagcgcggc ccagaccgtt gccggtcgca
      181 gcggcatttg aagatcggtg acgccatacg gcgccagggc gtccagcacg gcattgacga
      241 tcgcgggggg cgctccgata cagccggcct cacccgtgcc tttgacgccc aacggattag
      301 aaggcgccgg atgcaccaac cgctccgatg tgatcggcgg ggcgtcaccg gcccggggca
      361 acgcgtagtc catcaaagtg gaggtgagca gctggccctg ctccgagtat tggacaccct
      421 cgaacagggc ctgtccgaca ccctgcatga gtgagccgtg aagttggcct tccacgatca
      481 tggggttgat gacattcccg cagtcgtcaa ccgttacgag gcgctcgagc cgaacctgac
      541 cggtttccag ggcgacctcg acgaccgcga cgtgcacgcc gtaggggaat gtctgcgcgc
      601 cgggcacgaa ccattcttct tcgctcaaat cgatgccccg gtcgtgggcg gcagccgcca
      661 cctcgggcca acccatctcc gtgccaggta cgcctgcgat cgagaacgct ccgtcggcga
      721 gcaccacgtc ggcaggcgat gcctcgagga gctcggcggt cagttcgatg gctcgaccgc
      781 gcacggcatg cgccgtgcgc cagattgccg cagccccgag ctgggctgac ctgctggcga
      841 acgtcccggt gcctcgggcc acctcgtcgg tatcgccggc gaggtaggtc acgtcgtctg
      901 gggtcagccc caacgccgcg gctgccacct gtgcccaggc ggtctcgtgg ccctgacccg
      961 acgaggccga gcccgtccgc agagtggcct ttccctctcg gtcgatctca acccggccgt
     1021 actcgccggc atcgaccgcc cctccggcgc gctcgacgaa ggcgccgaac ccgacaccga
     1081 tcgggtcgcg tccttcggtc aggcgacctc gctgatcttc cctcagcttg tcgagatcga
     1141 cgagttcgac ggcgcgctgg agagccgctg catagtcacc cgagtcgtac agggcgcctg
     1201 tggcagtgcg gtaggggagg gaatcggagg ggatgaagtt ggtcagacgg acctcgaccg
     1261 ggtcgacgcc ggcggcacga gcgaaggcgt cgacagccct ctcgatggca tatgccgcct
     1321 cgggccgtcc ggctcctcgg tatgagcccg tcggagcggt gttggtgaca accgaagtcg
     1381 ttgtgagcga cacacttgcg atgtcgtaga ggccgagggc gaccagccgg gagaacatag
     1441 gaatcgtcgc gccgttgtgg gggtaggccc caacgtcggc gaccacgtcg atctccgctc
     1501 gttcgattcg gccctccgac gtccccatga gcctgactcg atgcacagag ccgcgtccat
     1561 gggttccgcc gctcagatgc tcgcgacgtc tctccgccca catcaccggc cgccccagcc
     1621 ggagggccag tgccgccgtc accgtgtgct ccgggtaggt catgcccttc atcccgaaag
     1681 caccgccgac gtctgggacg acgacccgca tcctctccgg gtcatgtggg agcagttggc
     1741 agaggcgggc cctgaccccg tggggtcgct gatggctcac gtagaccgtg aggcgaccgt
     1801 cgtcttcggg aatgacgagg atggccaacg gttcgatcgg attcggtgcc agtcgttgat
     1861 tgatcacctc gacctcggcc acgacctcgt agacgtcttt gggcacgccg gcggagagct
     1921 cggaccgact aaccacgttg gtccccgcgc tttcgtgcag ggtcacttca tcctcgaggc
     1981 tcgcccttgg atcgactact gcggggagaa gctcgtagtc gacccagatc aggtcggcgg
     2041 catcgactgc ctgggccgcc gtatcggccg cgaccacggc gatcggctcg ccgacatacc
     2101 gcaccttctg tgatgcgagg tgcggtcggg ggaaatcggg cgcaggcacg accgtgctgt
     2161 cacccgggat gtcggcgagg gcgaggtcgg cggccgtgaa cgcaccgacc acgccgggcg
     2221 agtcgaccga ggtgtcgatg ctcgtgatgc gagcgtgcgc ctcggtgctg cgaacgaagg
     2281 cgagatggac cgtccccgca ggtgccaggt cggcgacgta gcgcccggag ccggtgacca
     2341 gccgacggtc ctccagacga agatgagaca gcggctcgaa ataggggatg tcgtccacgc
     2401 cgcccctttt atagccgtcc gacccctcga caatcgattg cgcagtcgac cctatacttc
     2461 ccggccggtg gattcacggc ggcccaaagt ggcgattctc ggaacgcggt tcggtgattt
     2521 cgacgtcgaa cgtggagtgc tcggtgacgt cgacctggtt tctggtccgg ggcaaaccag
     2581 ccaggacgtg atcgaaatcg ccgggggggc ggacgtcatc ctggccggag cggcaccgcg
     2641 gttcgatgcc gcgacattgg aaaagctcga ctgccgtgcc atcgtccgtc tcggtgtcgg
     2701 tgtggacggc attgacctgg atcgtgcgcg atcgctgggc atgtgggtga tcaacgtgcc
     2761 cgattacggg atggacgctg tcgcgctgca tgctgtgacg ctggttctcg cgtcgctccg
     2821 ccggctcacc actgctgatc ggaagctgag ggaagggtca tggggggtga ccgacctgcg
     2881 gccgctgcgt cttccctcga gccttaccgt cggtgtcatg gggttcggaa ggatcggcag
     2941 acgggtggcc gtgcttctag ccgccctcgg tttcgggacg gtcctgatct ccgaccccgc
     3001 ggtggaggca tccgatgtca ccacgatgct gggggagact ccagctcgtc tcgtcggtgt
     3061 ggacgacctg ctcgccggat ccgatgtggt cacactccat tcccccccgc ccgacacggg
     3121 atacttgctc ggcgcgaccg agctgggaag gatgaaggag ggttcggtcc tcgtgaacac
     3181 cgctcgtggg gccctcgtcg acaccggcgc gctggtcgcc gcgctcgatg gcaaccggcc
     3241 cggctatgcc gcgctcgacg tgttcgaaca ggaaccaccc gatgtgtcga tcttcgagcc
     3301 agtcgccgac cgattgatcc tgaccccgca catgtcctgg tacacagagg agagcgagct
     3361 cgcccttcgg cgccaggccg ctgccgaggc gcgtcgcatt ctcaactcgg aggagccggt
     3421 ccacgccgtg gtcacgcccg aagcggccga gattcgatga ccatggcggg cagatccatc
     3481 aacgacctgg cctcgccgga tgtcgccgac atgatcggtc gcaccgggct ggtggtcatg
     3541 ccgttcggga gtgtcgagca gcacggcccc catctcccct gcgggaccga caccatggcg
     3601 gcagatctcg ttgctgccga gctggccgag cgtctcgatg cgatcctggt gccggccggg
     3661 ccgtacggtg tcaccccgat ccacgccggt catcccggca cgatctccct acggagagac
     3721 gtgttcgagg cctacctggc caacctgtgc caagagatgg ttgccatggg ggcgaggacc
     3781 tttgtattcg tcaactggca cgagggcaat atcgcctcgc tcgatgcggt agccacagag
     3841 ctccaggacc gtgaggacgt cctcgtcgtc accgctcacg cctgttacac ggctcagcgc
     3901 gtctactccc cggcaggcgg ggaactcacc catggcggcg ggatcgagac cctcgcagtg
     3961 ctcgctcacg acccagatct gttccacgcc gatcgggtgt acgagccgca caggccgccg
     4021 ggggcggacg agttggacac gatgcgtcgc tcccgcgagg tgtacggcta cataacagac
     4081 gtgactgaga tcgccgatga tggttggtac ggcaacccgg agtgggcgga ccaagcacgg
     4141 gccggacagt tcgccgccgt ggtgtccgac gagatcgccg tcgcggtcga atccatcctg
     4201 gagatgcgga ggcgcggaac gtgactgtcg ttggccccgg cgagctcgag tttcgggact
     4261 tcccggggag aaccaccgcg gacccgttca gagcggcggg ccaaggggct tccacggttc
     4321 gccaggtgat catcgagcac gtgccgtcgc ggtcgccaca tcgccacccc catagcgagg
     4381 aaatcgtgta tgtggtggct ggtcgggggc gcctctgggt ggacggggtg gtgcattcgg
     4441 taggacccgg gtcttggtat cggattccga tcgggacccc ccacgcgacc atggccgacc
     4501 ccaatgagcg aatgtccctc gtgtgcttct tcccacatga tgacttcgca agcaacatcg
     4561 aggagctcga tgtcgtcctc gaggtggatg gagaggcgaa cgatgagtga gcgcgtcagg
     4621 ctcgcctcta taggcctggg ccgatgggcc agggtgctcg ccagaggggc gcaacgaggc
     4681 gaggtgatcg aactggtgag ctgtttctca cgctcacccg agagtcgcct cgagttccag
     4741 acggagttcg gtatcccccg cgccgcaccg tcgctcgacg agctgctctc cgacccggag
     4801 atcgaagggg tcctggtcac gacgccgaac gacactcaca agaacgtcat cctcgagagc
     4861 ctcgacgccg gcaaggccgt gtacaccgac aaacccatag cccacacgat ggaagatgca
     4921 ggccagatag cgagagccgc cgccgcctcg gggcggccat ttgcggttgg tcacagcgcc
     4981 cgacggctcg gagggcaccg ggccatgaag cagtgggtcg agaccggcaa gatcggcaag
     5041 gtctcgctcg ccgaggcgaa cttctcgaac gagcgcggtt tggagttgac caccgacacc
     5101 tggcgctggt tcgccgacaa gagccctgga ggggcgatga tccagctcgg tgtccatcac
     5161 gccgacaatc tccagtatct cttgggcccc gtcgccgcag tgtcgagcca tgcccggagg
     5221 ctgtacacga aggctgaggt gccggacgcc atcatgtcga tcctcgaatt cgagtcgggt
     5281 cctctgggat acctgggcac gggttgggcc tcgccgggcg tataccagct tcggctccaa
     5341 ggcaccgagg cgaatctctt ctacgacctc gatttcaaca actgggatga gtcacacctg
     5401 gcggactcga agagcaccct cgtcagtcag gcctaccggg agtcgacccg ccccaatgtc
     5461 gatatacctc acacggacat gttccgggag cagctcgacg agtttgcgtt ggccatccgg
     5521 ggggaagcgg ggatcgaggt gggcgcgctc gaggcgatcc gggccctcgc cgtggttcat
     5581 gccgctctcg agtcctcgaa ccgcaacggt gaggccgtgg ctgtagcacc gatggtggag
     5641 agagcaggag cactcgagtg agggttgatc ctgccacgac ccgaaccatc catcacccag
     5701 acgcggtcat ccaggaagtc ctcgacatct gggcggctcg ctttcttcac gggcagatcg
     5761 cccttggtga cctgacggcg actgtcgaga gaatcagtac ctggtccgat tggggaccgg
     5821 aatggatgaa gaccgcccgg gtgcacgagg agctgggcga gcaggcttgg gaggaagggc
     5881 gccgcatctc ggcggtggcg gcgtttctca acgcggcagc ttgttaccac ttgtcctatt
     5941 tcctctcggt cgacgacgag aaggcgcacg ctcatggtct cgccaagatg gtggagtgtc
     6001 atgatcgggt tctcccgtac atgcagcccc aggtcgagaa ggtcagaatc ccgtttccag
     6061 acgccgatct cgttgggctt ctctcggtcc cgtcggttgg cgggccatct ccggtcgtga
     6121 tcttcctgcc cggtctcgac tcgaccaagg agcaacgtca tggcggtcgg ggagccctcc
     6181 tccgacgtgg catggcggtg ctcagcctcg atgggccagg gcagggggag accggcgcca
     6241 agctgcccat acgccacgac tacgaggtcg cagtgtctgc cgccatcgat tacctggaac
     6301 gtcgttacga ggtcgacgcg acgagagtcg gcctgatcgg ggccagtctc ggcggctact
     6361 acgcatgcag ggccgcagcc ttcgagcctc gggtcaccgc ggcggttgcc aattgcgggc
     6421 catacagctg gatcgaatgc tgggatgagc tccctgaggt aaccagggga gcgttccgca
     6481 agtactcatg gtctgcctcg gacgaggagg ccagagagaa ggcgaaggct ctggatctca
     6541 ccggcgtggc tgaggagatc acccagcccc tggtcgtggt gcacgggatg ctcgatccgc
     6601 tgataggtgt agaacatgcc agtcgaatcg ccgacgaggc tcccaacgcc atactgatca
     6661 ccgtcgacga cgccactcat ggcgtcagca accttggata caagttcgca ccgtgggtgg
     6721 atgactggat ggcggggcag ttgggcggcg ccatcacctg acccgggatc agatcacgat
     6781 cagctcgtgg gggaacttgg tcactttctc atgcccgccg tcggtgagca cataggtgcg
     6841 cccgaagaaa atcccgaaca gaccgtccgg agtgatgatc gtcggctcca gcatcgccac
     6901 ggtcccgggg gcgagctcga gctcggtctc atcggcaacg acgtgatccc acatcacgtg
     6961 cggggggtgg ctgacgaagt cgatgcaatg gaggtacaag gacctcgagt cgtaaccctt
     7021 gtcgcggaag aagtctcccg ccttcctgac gtcgtcccag ctcttgcctc cgaccaactg
     7081 ctcggccatg agctcgaatc cgggcaccac gacctcgtcg aagaactcgc ggatccacga
     7141 cggcggctcc ccgatgcaga acggggttcc cagctgcacc gagtaaccgt tgtagccgca
     7201 ggcgagctcg ttgatgatga tgtcgccttt ctcgagccgt cgactggatg gccatgggtt
     7261 gccgaacacc atgccggggt cgtccatccg cgtggacccg atgatgttga agttggtctg
     7321 tcccccgccg tccatcacag cgtgggtcgc ggccgcagcc agctggtact cggtgacccc
     7381 gggccgggca atggcgatca tggcgtggaa ggcttggtcc atcagctgcc ccgccttacg
     7441 gacacactcg atctcctcgg ggctcttgac atgcaccagc tcgtggaaga agtcaccgac
     7501 cctctccagg gtggcgttgg gtagcccgtc ccggaggcgc tcccactcgt cgaccggaag
     7561 gtgatggccg tagacggggt cgacgcacga gatcccgatg gtcgcgtcag ccagccccca
     7621 ttcttcgatc aaatcgacga tcacctctcc cgagtgcccc catcgagaat gcctcacgtc
     7681 tttcaggggc gtggctcgtc ggactgcctc gcgatgggcg ccgcccggcc cgcagatgag
     7741 caccggctcg ccctcgggag gtacgactgc ataagcggac atgccgtgcc actcccagtg
     7801 attgctgagc caccgcatcc cgcctcccca gctccagtga ttcggcccgc ccgaggcaat
     7861 caggacatct aggccgagac gcttcatctt ctcctgggtc gcggtgaaac ggcgctggta
     7921 ttccgcatcg gagaatcgct cgtatacggc atccgagtac cagggggtgt tcgccacccg
     7981 gccgaagacc tcggtgtgca tcttccccac cgcatctttg aagtcgacat cacccggtgt
     8041 catcgtgtcc tccttcaaga agcaactgag ccgagcaggc cgagcccgtc gaggtcggac
     8101 ctcagctcct ccattcgctc gtcgccgagc ggtatcaacg ggggccggac cgggccctgc
     8161 tccaaaccca tcagacccag ccaggccttc agctccgcga tcgggatcgc acccgcaccc
     8221 tgggccagcc attggtcgcg catccatttc tcgaatgcac tccgctcccg gaccaacgtc
     8281 ccctgcaggg cccaggcccc ctcggtgtct ccggcgtcag cgagacgtgt gtagtcgacg
     8341 atgggacggt gcgcgggtgt ctgatacatg gcgagcgccg gggtggacat gagcgaccgg
     8401 aaaccatgtt cgaggtgcag gccgagccaa tcgccctcat cggcgtcgca taccacgatc
     8461 cggtcgccga ccaggtcctg aacctggagc agatggtcca ttggcttggg gttcttgatc
     8521 ccgcacacgg tctcgagatc actgagctct tcgatcagtt gggggctcaa cgaatacccc
     8581 gagtatgaag tgttgaagag gaagaacggc acagaggact ccgcggtgag acgccgatac
     8641 cagtcccgga cgatgtcatc gctgg
//
LOCUS       DASTXO010000132         3433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_744660, whole genome shotgun sequence.
ACCESSION   DASTXO010000132 DASTXO010000000
VERSION     DASTXO010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744660"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2288)
                     /locus_tag="VFU96_02790"
     CDS             complement(<1..2288)
                     /locus_tag="VFU96_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEU5112215.1"
                     /translation="MDATREGSGFEPTNHDPRLRVFVSSTLGELKSEREAAKSAIKTL
                     RLDPAGIDLGGRDGQTEPSIGDSDIFVGIYWQSFGWESDLEREYDEGSGIPGLVYVKE
                     PAPDRDPGLSALLDRIRTKARSAVRTFESPGELAEMMVEDLAGLISERFTAIGPHPGA
                     LPGGTLTFMFGDLAGSTTLVEQLGDQYADVLAGYHQVVADATLSHDGTVLDLEGERLF
                     SVFQDAFQAVSATVEIQKSLDERVWPASAAVRARLGLHTGTARIGSGGYVGLDVHRAS
                     RVASSAHGGQIVVSAPVRELVEGQTESVGWEIKELGSFALKGLSRAEKLFQVVAPGLR
                     DGFPPPRARSITRVRLPTPQNVLVGREKELAELTEMMGRPNVRLATIVGPGGIGKTRL
                     ALSVAEDCSAHFPDGVFFVNLAAITDPSQVLLAIGDAAGIPIEGDAIDALSSEFQDQQ
                     VLLVIDNFEHVAAGGSSVADLLGRSPGLKVLATSRVPLRIMSEYEYTLEPLGLPPTGS
                     DRPDSISASDAVRLFIDRAIAVIPGFSINEENASPVASIVRKVDGLPLAIELAAARLR
                     MLSPGALSERLTRSLDALGSGAADVPSRQRTLDAAIDWSYQLLDRDEQALFCRLCVFQ
                     GGFTVESAQEVAVEEGDAVDQLMVLVENSLVLPAQGGEGRLRMLAPMREFGLRHLHER
                     GEYEWMKDRHADYYTRVADGALEDLRGTKQASVVERFAADWNNIDVAAEWLMHRGDDA
                     SLVKMSFGIWVFLWIGNHLKDGA"
     gene            complement(2332..2973)
                     /locus_tag="VFU96_02795"
     CDS             complement(2332..2973)
                     /locus_tag="VFU96_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112216.1"
                     /translation="MKYLIPVTGERLPRRIVQLIIGLVLFGVGIGLMLQSDLGVPPWD
                     VLHQGLARRFGLTVGLWSIIVSVLVLLLWLPLRQPYGLGTLLNAIIIGVIIDLTGAFI
                     PAAGGTGVAWSMLVGGVLLIGIASGLYIGANLGPGPRDGLMTGIARRGPSIRLTRAVI
                     EISVLTLGALLGGTFGIGTIVFALAIGPLVQYFLPRWDVSPHPTTDAWEHLDR"
     gene            complement(2975..>3433)
                     /locus_tag="VFU96_02800"
     CDS             complement(2975..>3433)
                     /locus_tag="VFU96_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5112217.1"
                     /translation="EMLDETFQILHGLMTEEVFDFRGKHRAITSARFEPKPVQTPRPP
                     FVVGGSGPRRTIPLAARWADQWNYPDFTSEPDLFVPALRRFEEACEEIGRDRSEIEVS
                     VQFRYPGDPDQTADLIAAYRELGAEHVLVSFMPPTSIDLPSRLAETLGGR"
BASE COUNT          618 a         1127 c         1147 g          541 t
ORIGIN      
        1 gcgccgtcct tcaggtggtt gccgatccag agaaagaccc aaatgccgaa ggacatcttg
       61 accagagagg cgtcgtcccc cctatgcatg agccattcgg cagcgacgtc gatgttgttc
      121 caatccgcgg cgaatctctc cacgaccgac gcctgcttcg tcccgcgaag atcctcgaga
      181 gcgccatcgg cgacccgtgt gtagtagtcg gcgtgccggt ccttcatcca ttcgtactcg
      241 ccccgctcat ggagatgacg caggccgaac tcccgcatcg gtgccagcat cctgagccgg
      301 ccctcaccgc cttgggcggg gaggacgagg ctgttctcga ccaacaccat caactggtcg
      361 acggcatctc cctcctcgac agccacctcc tgcgcggatt cgacggtgaa gcctccctgg
      421 aacacgcaca accggcagaa gagagcctgc tcgtcgcgat cgaggagttg gtaactccag
      481 tcgatcgcgg cgtcgagggt ccgctgtcgg ctcgggacgt cggctgcccc ggacccgagc
      541 gcatcgagcg acctggtgag gcgctccgag agcgcccccg ggctgagcat gcggagacgg
      601 gctgcggcca actcgatggc caagggcaag ccatcgacct ttcgcacgat cgacgccacc
      661 ggcgaagcgt tctcctcgtt gatcgagaac ccggggatca ccgcgatggc gcggtcgatg
      721 aacagacgaa cggcatcgga agccgagatc gagtcgggcc ggtcggaccc ggtcggaggc
      781 aggccgagcg gctcgagtgt gtattcgtat tcgctcatta tccggagcgg gacccggctg
      841 gtggcgagga ccttgagccc ggggctccga cccaggaggt cggcgaccga cgagccgccc
      901 gctgccacat gctcgaagtt gtcgatgacg agcaggactt gctgatcctg gaactcgctg
      961 gagagcgcat cgatggcgtc accctcgatg gggatgccgg ctgcgtcccc gatggcgagc
     1021 agcacttggc tcgggtcggt gatggcggcc aggttgacga agaagacccc atcggggaaa
     1081 tgcgccgagc aatcctcggc gaccgacagt gcgaggcggg tcttcccgat cccacccggg
     1141 ccgacgatcg tggccagtcg gacattgggg cgtcccatca tctcggtcag ctcggccagc
     1201 tccttctccc gccctaccag gacgttctgg ggcgtgggga gccgaacccg tgtgatcgac
     1261 cgagcccggg gtggcgggaa cccgtctctc aggcctgggg ccaccacctg gaacagcttc
     1321 tcggcgcggg agaggccttt gagggcgaag gagcccagct ccttgatctc ccatcccacg
     1381 ctctcggtct gcccctcgac cagctcgcgc accggggcag agacgacgat ctggccaccg
     1441 tgagcagagg aggcaacccg ggaagcccga tggacgtcga gcccgacata tcccccggac
     1501 ccgatccggg cggtaccggt gtgcagcccg aggcgggccc gcacggctgc cgacgccggc
     1561 cacactctct cgtcgagcga cttctggatc tcgaccgtgg cgctgacggc ttggaaggca
     1621 tcctggaaga cgctgaagag acgctcccct tcgagatcca acaccgttcc gtcatgactc
     1681 aaagtcgcat cggcgacgac ctggtggtag ccggcgagga cgtcggcgta ctgatctccc
     1741 agctgctcaa cgagggtggt cgacccggcg agatcgccga acatgaaggt gagcgtcccc
     1801 cccggcaatg caccggggtg cggcccgatc gcggtgaacc gctccgaaat cagcccggcg
     1861 aggtcctcca ccatcatctc ggcgagctct ccaggggact cgaaggtccg gacggccgat
     1921 cgcgcctttg ttcgaatgcg atccagcaag gcgctcaggc cggggtctcg atcgggggcg
     1981 ggctccttga cgtagacgag accggggatg cccgagccct cgtcgtattc ccgctcgagg
     2041 tcggactccc agccgaagga ctgccagtag atgcctacga aaatgtccga gtcgccaatc
     2101 gatggctcgg tctggccgtc cctgccgccg aggtcgatgc cggcaggatc gagacgcaac
     2161 gtcttgatcg cagattttgc ggcctctcgc tctgacttga gctcaccgag cgtggacgac
     2221 acaaagaccc tcaatctcgg gtcgtgattg gtcggctcga agccgctccc ctccctcgtc
     2281 gcatccacga ggcacaacgg tagtgagttg gccggcgtta cctgccatgg ttcagcggtc
     2341 gagatgctcc caggcgtcgg tagtcgggtg gggggagaca tcccagcgcg gcaagaagta
     2401 ctggaccagc gggccgatgg ccagggcgaa gacgatcgtg ccgatgccga aggttccgcc
     2461 gaggagggcg ccgagggtga ggaccgatat ctcgatgacg gcgcgggtga gtctgatcga
     2521 cggtccccga cgcgcgatgc cggtcatcag accgtcgcgc gggcctggcc ccaggttggc
     2581 accgatgtag aggcccgatg cgatcccgat gagaaggacc ccgcctacca gcatggacca
     2641 cgcgactccg gtcccccccg ccgccgggat gaacgccccg gtgaggtcga tgatcacgcc
     2701 gatgatgatt gcgttgagca gggtgccgag gccgtagggc tgacgcaacg gcagccagag
     2761 gaggagcacc agcaccgaga cgatgatcga ccagaggccg accgtgagcc cgaagcgtcg
     2821 ggccagaccc tgatggagga catcccacgg cggcaccccg agatccgact gcagcatcag
     2881 cccgatgccg acaccgaaca ggaccagtcc gatgatgagt tggacgatgc gacgcgggag
     2941 gcgctcgccg gtgaccggga tgaggtactt cacatcatcg acctcccagg gtctccgcga
     3001 gccgggacgg gagatcgatg cttgtcggtg gcatgaagct gaccagcacg tgctctgccc
     3061 ccagctcgcg ataggccgcg atcaggtcgg ccgtctggtc cgggtcgccg gggtaacgga
     3121 actggaccga gacctcgatc tcggagcggt ctctcccgat ctcctcgcag gcctcctcga
     3181 atcggcgcaa ggcgggcacg aacaggtccg gctccgaggt gaagtccggg taattccatt
     3241 ggtcggccca gcgagcggcc agcgggatgg tccggcgcgg gccggatccc ccgacaacga
     3301 acggcggtct cggcgtctgg accggtttcg gctcgaagcg tgccgaggtg atggctcgat
     3361 gcttcccacg gaagtcgaac acctcctcgg tcatgagccc gtggaggatc tggaacgtct
     3421 cgtcgagcat ctc
//
LOCUS       DASTXO010000133         3702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_413325, whole genome shotgun sequence.
ACCESSION   DASTXO010000133 DASTXO010000000
VERSION     DASTXO010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413325"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..2032
                     /gene="typA"
                     /locus_tag="VFU96_02805"
     CDS             206..2032
                     /gene="typA"
                     /locus_tag="VFU96_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU5112218.1"
                     /translation="MPTQSVRNVALIAHVDHGKTTLVDSMLSAAGVFAAHQERVERVM
                     DSTDQERERGITILAKAASIQWRDTKINLVDTPGHADFGGEVERALAMVDGILLLVDA
                     ADGPMPQTRYVLSKALERHLPAVVVINKVDRPDARPKEVADEVYELFFDLDAKDHHID
                     FPIVSTVARSGRAVVGIGVPSEGSDLSALLDAIVDTIPAPAGDPEAPIQALVTNLDAS
                     DYLGRLAIGRVFEGTLRQGSTIALCHSNEEEPPIRRKLTQLMGFSGLGRAETEEVRAG
                     DLFVVAGFPEVEIGDTFADSANPEPLPRLIVDEPVLKMTFGVNTSPLSGQDGKFLTSR
                     QIQDRLRREILGNVSIRLGATSSPEVVEVAGRGELQLAVLIESMRREGFELQVSRPEV
                     VIKQFEGKPHEPVERAVVDVPDEYVGAVTQAAAPRKGTIAELSPGDPGRSIITIVAPA
                     RGLLGFRSLLMTTTRGTALVHQHHDGWMPWVGELPHRIGGAMISDRAGTSTAFALDNL
                     QKRGDLFIGAGEVVYEGMIIGEASRPDEMVVNAARPKQLTNIRTHASDEAINLRPPRE
                     LTLETAIEWISDDELVEVTPTAIRVRKRYLSEPDRRRYGKKS"
     gene            complement(2039..2227)
                     /locus_tag="VFU96_02810"
     CDS             complement(2039..2227)
                     /locus_tag="VFU96_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112219.1"
                     /translation="MFLAQCDILGQEALIWASDLHGIINSDAGIVVRYRCVCGETAEM
                     LTGSGSDVRVSVHLGAAA"
     gene            complement(2208..2384)
                     /locus_tag="VFU96_02815"
     CDS             complement(2208..2384)
                     /locus_tag="VFU96_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112220.1"
                     /translation="MIGHPAIAYLVAQRILDERREEANAYRKAKAVKKTRTIKLGSYR
                     LTLSKEVRGVPRPV"
     gene            2462..3010
                     /locus_tag="VFU96_02820"
     CDS             2462..3010
                     /locus_tag="VFU96_02820"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HEU5112221.1"
                     /translation="MNFSHYSDQTVELAVDLVNTDELTGDEIATLADLETFLTQYESL
                     LPPDQQPATEKDLKKLHRLRDDLRAIFTAPDTETAVAQLNRILEKNNATPRVSMHDGE
                     PHLHYEPVNAPMASRVAAIAAMGLAGVIVENGISRFGSCSASNCRDVFVDATRNRSRV
                     HCCHSCSTREAVAAYRKRQNAG"
     gene            3056..3367
                     /locus_tag="VFU96_02825"
     CDS             3056..3367
                     /locus_tag="VFU96_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112222.1"
                     /translation="MATGSPAVTRHPIWWWVHDIVVGFLSGVGVGAVAGLFLNRLVEN
                     NVVVLAGAVLGAAAGIWVLLQNHRRAERFMSGVVVVSWLLLILSAGFLGLFIWAILNF
                     E"
     gene            complement(3354..>3702)
                     /locus_tag="VFU96_02830"
     CDS             complement(3354..>3702)
                     /locus_tag="VFU96_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112223.1"
                     /translation="YDQSWQYVSRGLELFSEHHDVSGVVLLLAACAGLALILGDRDRA
                     HRLAGAFHGLRISSGTEIVLNELNRIEALDYESMEALTGEAAIPYREGRAMSLEAAVA
                     YALTGPADVTTRS"
BASE COUNT          693 a         1173 c         1154 g          682 t
ORIGIN      
        1 cttcgctccg agccttccag gtcacctgca tggggccggc tgcgccgctc tcgagggtcg
       61 cggtcccgga cccttcggtg agatcacctt cccaatgggc gcgaccagtg ctgtcaaggg
      121 ccatttgctg tcctttcggt ctggacgtcc gagcctaacg ctccaccggg tcccgatcac
      181 cggcgtgccg ataccatcgg tgacgatgcc cacccaatcg gttcgtaatg tcgcgctcat
      241 tgcccatgtc gaccatggca agacgactct ggtcgactcc atgctgtccg cggccggcgt
      301 tttcgccgcc caccaagaga gggtggagcg agtgatggac tcgaccgacc aggaacgcga
      361 gcgcggcata accatcctgg ccaaggcggc gtcgatccaa tggcgcgaca ccaagatcaa
      421 cctggtcgac acgccggggc acgccgactt cggtggggag gtcgagcggg cgctggccat
      481 ggtcgacggg atcctcctgc tggtggacgc tgccgatggg ccgatgccgc agaccagata
      541 tgtgctgagc aaggcgttgg agcgacatct tcctgcggtg gtggtgatca acaaggtcga
      601 ccggcccgat gctcgaccga aagaggtggc cgacgaggtc tacgagctgt tcttcgacct
      661 ggacgccaag gaccatcaca tcgatttccc catcgtctcc accgttgccc gcagcggccg
      721 cgcggtggtg ggaatcggag tgccttccga aggatcggac ctctccgccc tgctcgatgc
      781 catcgtggac accatccccg ccccggcggg cgaccccgaa gcgccgatcc aggcgttggt
      841 caccaatctc gacgcatccg actacctggg ccgtcttgcc atcgggaggg ttttcgaagg
      901 gacgctgcgg caggggagca ccatcgccct ctgccacagc aacgaggaag agccaccgat
      961 caggcgcaag ctgactcagc tgatgggatt ctccggtctg ggccgggccg aaaccgaaga
     1021 ggtgagagcc ggagatctct tcgtcgtcgc cgggttcccc gaagtggaaa tcggggacac
     1081 gttcgccgac tcggccaacc ccgagccgct tccccgactg atagtcgacg agcccgtgct
     1141 caagatgacg ttcggtgtga acacctcgcc tttgtccggc caggatggga agttcctcac
     1201 ctcgaggcag atccaggacc ggctccgacg ggagatcctg ggaaacgtct ccatccggct
     1261 cggagccacc tcttcccccg aggtggtcga ggttgcaggg cgtggcgagc tgcaactcgc
     1321 tgttctcatc gagtcgatgc gccgagaggg cttcgagttg caggtgagtc ggcccgaagt
     1381 cgtgatcaaa cagttcgagg ggaagcctca cgagccagtg gagcgagcag tggtcgacgt
     1441 cccagacgag tatgtcgggg cggtcaccca ggcggcagca cccaggaagg gaacgatcgc
     1501 tgagctctct cccggcgacc cgggtcgctc gatcatcacc atcgtcgccc cagctcgggg
     1561 cctcctcggt ttccgctcac tgctcatgac caccacacgg gggacggcgc tcgtccatca
     1621 gcaccacgat gggtggatgc cttgggtggg cgagctgccc catcgcatcg gtggagccat
     1681 gatctcggat cgcgccggaa ccagcaccgc ctttgctctg gacaacctcc agaagcgagg
     1741 cgatctcttc atcggggccg gtgaggtggt ctacgaaggg atgatcatcg gcgaggcatc
     1801 acgccccgat gagatggtcg tcaatgcggc ccgaccgaag cagctcacaa acatcaggac
     1861 tcatgcatct gacgaagcga tcaatctcag acctccccgg gagttgacgc tggaaacggc
     1921 catcgagtgg atctccgacg atgagctggt ggaagtgaca ccgaccgcga tccgtgtccg
     1981 caagcgctat ctcagcgagc ccgatcgtcg gagatacggt aagaagagct gagatccctc
     2041 aggccgccgc gcccaggtgg acgctgacac gcacgtcgga tcccgaaccg gtcagcatct
     2101 cggcagtctc accgcacacg caccgataac ggaccacgat gccggcgtcg ctgttgatga
     2161 tcccgtgcag gtcggaggcc cagatcaagg cctcttggcc gaggatgtca cactgggcga
     2221 ggaacaccac ggacctcctt gctcaaagtg agccgatatg agcccagctt gatggtgcga
     2281 gtcttcttga cggctttcgc cttgcggtat gcgttcgctt cctcgcgtcg ttcgtcgagg
     2341 attcgctgcg ccaccagata tgcgatggcg ggatgcccaa tcatgttggg aacctcctct
     2401 tgacgtaata accgttcatc cgtacacttg ttaccatatc gaccgggatg taataagtca
     2461 aatgaatttc tctcattact cagaccagac agtggaactg gcggtggatc tggtgaacac
     2521 cgacgagctg accggtgacg agatcgccac gctggccgat ttggagacat tcctgaccca
     2581 gtacgagtcg ctcctccctc ccgaccagca gcctgccacc gagaaggact tgaaaaagct
     2641 tcacaggttg cgtgacgatt tgcgtgcgat tttcactgcc ccggacaccg agacggcagt
     2701 cgcccagctc aatcggatcc tggagaagaa caacgccacc ccacgggtct ccatgcacga
     2761 cggcgagcca catctgcatt acgagccggt gaacgctccc atggcatccc gggttgcggc
     2821 catcgcagcg atggggctgg ccggtgtgat cgtcgagaac gggatcagcc ggttcggaag
     2881 ctgcagcgcc agcaattgcc gcgatgtgtt cgtggacgcc acccgcaacc ggtcacgggt
     2941 gcattgctgc cacagctgct cgacgaggga agccgtggcc gcctatcgga agcgccaaaa
     3001 cgccggctag agggatcgcg ccccccaaaa tccggggttg ccttagattg caaccatggc
     3061 gaccggctct cccgctgtca cccggcaccc gatctggtgg tgggtccacg acattgtggt
     3121 gggtttccta tcaggcgtgg gagtgggtgc cgtcgccggt ctcttcttga atcgattggt
     3181 cgagaacaat gtcgtggtgc tggccggtgc cgtgctcggc gccgccgccg gcatctgggt
     3241 cctcctccag aatcatcgga gagctgagag gttcatgagc ggcgtagtgg tggttagttg
     3301 gctcctactg atcctctcag ccggctttct cggcctgttc atctgggcga tcctcaactt
     3361 cgagtagtca catcggccgg gcccgtgagg gcgtaggcaa cggctgcctc caggctcatg
     3421 gcccgaccct cacggtacgg gatggccgcc tcgccagtca gcgcttccat ggattcgtag
     3481 tcgagtgctt cgatccgatt cagctcgttg agcacgatct cggttcctga agagatccgc
     3541 aggccgtgaa acgcgccggc cagcctgtga gctcggtctc gatctccgag gatgagcgcg
     3601 agcccggcac acgcagcgag gagcagcacc accccgctga cgtcgtggtg ttcggagaac
     3661 aactcgagcc ctcgcgatac gtattgccag gactggtcgt ac
//
LOCUS       DASTXO010000134         4040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1297191, whole genome shotgun sequence.
ACCESSION   DASTXO010000134 DASTXO010000000
VERSION     DASTXO010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297191"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="VFU96_02835"
     CDS             complement(<1..934)
                     /locus_tag="VFU96_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112224.1"
                     /translation="MTAPRLFVRGNPWPVVLTLAGISLVILIVMNPWWIVSSTTPTGG
                     DMGAHVLGPAFLRDSLLPEGRIQGWSNDWFAGFPAFYFYFPLPSLTIVFLDLFLPYGV
                     AFKIVTVLGLLAMPPAAYYLARSMRLGKHISVIAAGAGVVFAFFESYSIYGGNIASTL
                     AGEFAYSWSFALSLIYLGLLIRAVRDDRRYLKWAALALAATALSHVLTTIVVIFASLF
                     VLPWRKGFWRTLAIWLWGFAIAAFWALPLIARIGFSSDMSWTPLSRWEEIFPVELWLL
                     LPLAIPGAVWISRRTSRAAPLLAATILPVLYFPLP"
     gene            complement(931..1107)
                     /locus_tag="VFU96_02840"
     CDS             complement(931..1107)
                     /locus_tag="VFU96_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112225.1"
                     /translation="MNCPQCSQPDVIEIEHVLPDGTQVLFFACHNCEERWWNRDGEDI
                     DITQVLEIVRQHRA"
     gene            complement(1154..1432)
                     /locus_tag="VFU96_02845"
     CDS             complement(1154..1432)
                     /locus_tag="VFU96_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112226.1"
                     /translation="MALDRDLLDAVKDLDEHDLRRLLILTRAQLQGSGALGTDDHPEV
                     HLRQQAVRCGKTGCTRCPHGPYWYAYWTENGRRRSRYVGRLLDEEALL"
     gene            complement(1432..1653)
                     /locus_tag="VFU96_02850"
     CDS             complement(1432..1653)
                     /locus_tag="VFU96_02850"
                     /inference="COORDINATES: protein motif:HMM:NF023430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 family protein"
                     /protein_id="HEU5112227.1"
                     /translation="MILSCARCGSPAAAVMTFSYPDSSVWMDDLVNGVDRHGYAMCAD
                     HADRMIPPLGWTLTDRRTVTRLFAPLSVA"
     gene            complement(1686..2042)
                     /locus_tag="VFU96_02855"
     CDS             complement(1686..2042)
                     /locus_tag="VFU96_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112228.1"
                     /translation="MNEIWLRLAIVVGVVLASLIIVVVMSRRPKVSALDRGVLDRGVY
                     LFTSSSCADCAGARARLEELLGRSSFVEISWEDRPALFTRLGIDAVPSTVVVGDDGSA
                     SLHPGMPGHDLGGVNP"
     gene            complement(2035..3327)
                     /locus_tag="VFU96_02860"
     CDS             complement(2035..3327)
                     /locus_tag="VFU96_02860"
                     /inference="COORDINATES: protein motif:HMM:NF040160.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5719 family protein"
                     /protein_id="HEU5112229.1"
                     /translation="MTKALLAILVAALTVGVLLIPSPEPPQAGEVAAVDPPAVAVCPV
                     EEGSGRTTTIGVASGVNGEGRFTAFAAGVSAGATTFSTGASGSAAIPIAEIAPLGTGA
                     GLAELPGQDVAAASVFVGAQTVAVEACLRTPVRQTLLAGGSTVSGERFQVQLMNPYAG
                     EAVVDLIVQSESGLEAAPQLRGILVPPRSSAIVDLAELLPGRQSLSIAVEVASGNVLT
                     VGRFDAGGDGALWHSVVPASDWYVPVPAGGLNGELVISTGVAAEMTFQVDVYGAQGVV
                     EGFQEGVVPSRGATSIQLAEMGLEGASALRVITTQPAAVFLRDISDAGVAITSGATVT
                     APTWLLPGGGLAPGGRGSAVILNAGLEEDSVVITALREQSIAQELSIPAGTVLQVPSI
                     EGGANSYSVRGSGDLVALWVTNTDTGKAESIGIPIVDE"
     gene            complement(3324..>4040)
                     /locus_tag="VFU96_02865"
     CDS             complement(3324..>4040)
                     /locus_tag="VFU96_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112230.1"
                     /translation="LSAGELVNGRLGLPAGDANERFAFASTLADPGSPGRVLLISADR
                     SLIPGEARPGPGIWYRTLDGQGTTLDEVWLPQPQAGDVQLADAIDRIASGADLRPGAL
                     LAPFAIDWVVVDGIETPLDPVLDSQVDLTPTPLVTGAKVFENPNSAPLAGVEDGVVWH
                     REGTAFAGETASGPVALRVNYSHGWSPQPEPVDWFTSVSAVDGKATFSASGYLSYAPY
                     ATAGLLLLALALVGWGRARR"
BASE COUNT          824 a         1308 c         1299 g          609 t
ORIGIN      
        1 gtgggagagg gaaatagagg acaggcagga tggtcgcggc cagcaagggc gctgctctcg
       61 atgtccggcg gctgatccac accgcccccg ggatcgccag cgggagcagc agccacaact
      121 cgacggggaa gatctcttcc catcgggaca atggcgtcca cgacatgtcc gagctgaacc
      181 cgatgcgcgc gatcagaggc aacgcccaga aggccgcgat ggcaaagccc cacagccaaa
      241 tggccagtgt gcgccagaaa cccttccgcc atggcaggac gaagagcgag gcgaaaatga
      301 cgacgatggt cgtcaggaca tgagagaggg cggtggcggc gagggcaaga gcggcccact
      361 tcagatatct ccgatcgtcg cgtaccgccc tgatgaggag cccgaggtag atcagtgaca
      421 gtgcaaacga ccatgaatag gcgaattcgc ccgcgagtgt cgaggcaatg ttccctccgt
      481 agatggaata gctctcgaag aatgcgaaca ccacccccgc ccccgctgcg atcacggaga
      541 tgtgctttcc gagtcgcatc gagcgagcca ggtaataggc ggcgggtggc atggcgagca
      601 ggcccaggac agtgacgatc ttgaacgcca ccccgtaggg gaggaacaag tccaggaaga
      661 cgatggtcaa cgagggcagc gggaagtaga agtaaaacgc gggaaagccg gcaaaccagt
      721 cgtttgacca tccttggatc ctgccttcag gaagaaggct gtctctgaga aacgcgggac
      781 cgaggacatg ggcgcccatg tctccgccgg tgggcgtcgt ggacgagacg atccaccatg
      841 gattcatcac aatgaggatc accagcgaga tgccggcgag ggtgaggacg accggccagg
      901 gattcccccg cacgaacaac cggggggcgg tcacgcgcga tgctgtcgga cgatctccag
      961 aacctgtgtg atgtcgatgt cctcgccatc acggttccac catctctcct cgcagttgtg
     1021 acaggcgaag aacaggacct gcgtcccgtc ggggaggacg tgctcgatct cgatcacatc
     1081 aggttgtgag cactgtggac agttcatctc gtctcgacac tggggagagg cacatggtac
     1141 gaccgtcgtg tccctacaac aatgcctcct cgtcgaggag tcgccccacg taacggcttc
     1201 gtctccgccc gttctcggtc caatacgcgt accaatacgg gccatgagga catcgagtgc
     1261 acccggtctt gccgcaccgc actgcctgct gccggagatg gacttcgggg tggtcgtcgg
     1321 tgcccaaggc acccgagcct tgtagctgcg ctctggtcag aatcagcagc cgtcgtagat
     1381 cgtgctcgtc gagatccttg accgcatcga gaagatcgcg gtcgagggcc atcaggccac
     1441 agagagcggg gcgaacaacc gggtgacggt tcgtcggtcg gtcagcgtcc acccgagagg
     1501 tgggatcatg cggtcggcat gatcggcaca catggcatac ccatgtcgat cgacgccgtt
     1561 gacgaggtca tccatccaga cagagctgtc cgggtaggag aaggtcatga cggcggcagc
     1621 cggcgaccca catcgggcac aactgagaat catgccggaa acggtagggg ttgggccaag
     1681 cctgttcaag gattgacgcc acccaggtca tgaccgggca tccccgggtg gagtgatgcc
     1741 gagccatcgt cgccgaccac cacggtggag ggaacggcat cgatgccgag tcgggtgaac
     1801 aacgcggggc ggtcctccca tgagatctcg acgaaagagc tcctgcccag caactcctcc
     1861 aaacgcgccc gagcccccgc acagtcggcg cacgatgagg aggtgaacag gtagacgccg
     1921 cggtcgagca cgccgcggtc cagggcagag accttcggcc ggcgactcat cacaacgacg
     1981 atgatgaggg aggcgaggac taccccgacg acgattgcca gtcggagcca gatctcattc
     2041 atcgacgatc gggatgccga tgctctccgc tttgcccgtg tcggtgttcg tcacccagag
     2101 cgcgaccaga tcgccggatc ccctgacgct gtaggagttg gcgccgccct cgatcgacgg
     2161 cacctgaagc actgttccgg cagggatgct caattcctgg gcaatagatt gttcgcgtag
     2221 ggcagtgatg acgacggagt cctcctcgag gccggcattg aggatcaccg cgcttccccg
     2281 cccaccaggc gccaaaccgc cgccagggag aagccacgtc ggtgcggtaa ccgtcgcgcc
     2341 actggtgatg gccacacccg catcggagat gtctcgcagg aagaccgccg ccggctgggt
     2401 tgtgatcacc ctcagagccg acgccccctc gagccccatt tcggcgagtt ggatgctcgt
     2461 tgctcccctg ctcggcacta cgccctcttg gaatccctcg accacgccct gcgcgccgta
     2521 gacatcgacc tggaaggtca tctccgccgc aacacctgtc gagatgacca gctcgccatt
     2581 gagccccccc gcaggcaccg gcacgtacca atcggacgcc ggcaccaccg agtgccagag
     2641 ggccccatcc ccaccggcgt cgaatcgacc caccgtcaag acattgccgc tcgccacctc
     2701 gacggcgata gaaagcgact gtctaccggg caatagctcg gcgaggtcca cgatcgcgga
     2761 ggatcggggc ggaaccagga tcccccgcaa ttgcggtgca gcctcgagac ccgactccga
     2821 ctgaacgatc agatcgacca ctgcctctcc ggcatatggg ttcatcagct gaacctggaa
     2881 ccgttcgcca ctcaccgttg atccacctgc gagcagtgtc tgccgaacgg gtgtgcggag
     2941 acatgcttcg acggcgacgg tttgggcacc cacgaatacg gacgccgccg cgacgtcctg
     3001 cccgggcaat tcggcgagac cggctccggt gcccaacgga gcgatctcag cgatcgggat
     3061 cgcggccgag ccagaggccc cggtggagaa cgtcgtcgcg cccgcggaca cgccggcggc
     3121 aaacgcggtg aacctgccct cgccgttgac accggacgcg acaccgatgg tcgtggtgcg
     3181 accgctcccc tcctcgaccg ggcacaccgc gacagcgggt gggtcgacag cagcgacctc
     3241 gccggcctgg ggcggctcgg ggctggggat gagcagcacg ccgaccgtga gtgcggccac
     3301 caagatggca aggagtgcct tggtcatcgc cgcgccctcc cccatccgac gagcgcaagc
     3361 gcgaggagga gaagaccggc ggtggcgtag ggcgcatagg acaggtatcc gctcgccgag
     3421 aatgtggcct tcccgtccac cgccgacacg ctggtgaacc agtccaccgg ctccggctgc
     3481 ggagaccaac catgcgaata gttgaccctc agcgcaacgg ggcccgaggc ggtctcgccc
     3541 gcgaatgcgg tcccttcgcg atgccagaca acgccgtctt caacacctgc cagcggggcg
     3601 gagtttggat tctcgaatac cttggctcct gtgaccagcg gcgttggagt caggtccacc
     3661 tgggagtcca ggacaggatc cagaggcgtc tcgatgccgt ccacgacaac ccagtcgatg
     3721 gcgaacggcg ccagcagtgc cccgggacgc aggtcagcgc cgctggcgat ccgatcgatt
     3781 gcatcggcga gttggacgtc accggcctgg ggttggggca accacacctc atcgagggtc
     3841 gtgccctgac cgtcgagcgt ccggtaccag atgccgggac caggccgcgc ctcccccggg
     3901 atgagacttc gatcggcgga gatcaagagc actcgcccag ggctccctgg atcggccagg
     3961 gtcgaggcaa acgcaaatcg ctcgttggca tctccagcag gcagccccaa acgaccgttg
     4021 accagctccc cggcagagag
//
LOCUS       DASTXO010000135         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_560391, whole genome shotgun sequence.
ACCESSION   DASTXO010000135 DASTXO010000000
VERSION     DASTXO010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560391"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VFU96_02870"
     CDS             complement(<1..784)
                     /locus_tag="VFU96_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5112231.1"
                     /translation="MSSVDAQVSVLDDLIARERLSFLDRAPKATEMGDRAGRSLAGGV
                     TSSWQISDPQPIWIERGEGSRVWDVDGNEYVDLHGGYGAMLAGHAHPAVVAAVSERVR
                     KGTHFAQPTADAIVVAEELTRRYGMTMWRFGNSGTEATMDAVHLMRAITGRDLIIKFE
                     GGYHGHHDSVMVSVWNEDGLGPETRPASMPAGTGIPSAIVDLTLVATFNDLDVVSQLF
                     DENEGRVAGVILEPILQNCGVLLPQPGFLQGLRDLTRKHDALL"
     gene            862..2052
                     /locus_tag="VFU96_02875"
     CDS             862..2052
                     /locus_tag="VFU96_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEU5112232.1"
                     /translation="MKPAPVDPDQISRVLDPFGSSLTFPADVYLSEDILAWERENLWQ
                     STWNCVGRLDELVTPGQLRAIDVGGEGVLLSRDSDGDLRAFSNVCRHRGHELAPIGDA
                     FDARQIRCPYHSWSYRFDGSLRTAPRFTQSDEFDTSEYPLIDLGVAVYGGWVWIDLSG
                     RAPAIEDHFGNLAEITRPYETDRLITAATHSYVVDANWKIIIENYNECYHCSSIHPEL
                     CEVTPPDSGYDHIPTGMWCGGTMDLKSHAVTMSLDGSSRGINFRNLTGKLPRQVWYLT
                     VIPNLLLSIHPDYVMTHRLTPVGTDQTHIECSWLFPPEAFELEGFDPAYAVDFWDITN
                     REDWAACEAVMRGVKNRGFRPGPLSGWEGTVDQVLNIMSHAYLGRGLVIPTVPTRELR
                     ETVT"
BASE COUNT          378 a          696 c          619 g          393 t
ORIGIN      
        1 tcaggagcgc atcgtgcttc cgggtcaggt cacgaaggcc ttgcaggaac ccgggctgcg
       61 ggagcaggac cccgcaattc tggaggatcg gctcgaggat caccccggcg acccggccct
      121 cgttctcgtc gaatagctgg ctcacgacgt cgaggtcgtt gaaggtcgcc acgagcgtca
      181 ggtcgacgat ggcggaagga atcccagtgc ccgcaggcat gctggcgggc cgcgtctcgg
      241 gccccagacc gtcctcgttc caaaccgaga ccatcaccga atcgtgatga ccgtggtaac
      301 cgccctcgaa cttgattatg agatcccggc cggtgatggc gcgcatgagg tgcacggcat
      361 ccatcgtcgc ttcggtgccc gagttgccga accgccacat cgtcatcccg tacctgcggg
      421 tgagctcctc cgcgacgacg atcgcgtcgg cagtcggctg ggcgaagtgg gtccccttgc
      481 gcacccgctc tgataccgct gcgaccaccg cggggtgggc gtggccggcg agcatggcgc
      541 cgtatcctcc gtgaagatcg acgtactcgt tgccgtcgac atcccacacc ctcgatccct
      601 caccgcgctc gatccatatc ggctggggat cggagatctg ccagctggag gtgactcccc
      661 ccgccagcga ccgcccggca cggtcgccca tctcggtcgc tttgggcgcc cgatccagaa
      721 aactgagacg ctcccttgcg atcaagtcat cgagaaccga gacttgcgcg tccacggagg
      781 acatgagcca ccttccttcc ggtatcctga ctcgccgttc aggatacact gagcgaacca
      841 cggcggcagg agagaatcgg gatgaagccg gcaccggtcg acccggacca gatcagcaga
      901 gtcctcgatc catttggatc gagtctgacc ttccccgccg acgtctacct ctccgaggac
      961 atcctggcct gggaacgcga gaacctctgg cagtcgacct ggaactgcgt ggggcgtctc
     1021 gacgagctgg tcactcccgg tcagcttcgt gccatcgacg tcggtggcga gggagtcctg
     1081 ctcagtcgag attcagacgg cgacctgcgc gccttttcca acgtgtgccg tcatcggggt
     1141 catgaactcg ccccgatcgg cgatgccttc gacgctcgcc agatccgctg cccgtatcac
     1201 agctggtcgt atcggttcga tgggagtctg cgcaccgctc cccgattcac ccagagtgac
     1261 gagttcgata cttccgaata cccgttgatc gatctcggcg tggccgtgta cgggggatgg
     1321 gtgtggatcg atctctcggg tagggcgccc gccatcgagg accatttcgg gaacctcgcc
     1381 gagatcacgc gaccttacga gaccgatcgg ctgatcaccg cggcaaccca ctcctacgtc
     1441 gtcgacgcca actggaagat catcatcgag aactacaacg agtgctacca ctgctcgtcg
     1501 atccatccgg agctatgtga ggtgacccct ccggacagcg gttatgacca catccccacc
     1561 gggatgtggt gtggcgggac gatggatctc aaatctcatg cagtcaccat gtccctcgac
     1621 ggatcgtcac gagggatcaa cttcaggaac ctgactggaa agctcccccg tcaggtgtgg
     1681 tatctcaccg tcatcccaaa ccttctcctc tcgatccatc ccgattacgt gatgacccat
     1741 cgcctcacgc cggtcggcac cgaccagacc cacatcgaat gctcttggct gttcccaccc
     1801 gaggcgttcg agctggaggg tttcgacccc gcgtacgccg tcgacttctg ggatatcacc
     1861 aatcgagaag actgggccgc ctgcgaggcg gtgatgcggg gcgtcaagaa ccggggtttc
     1921 cggccgggcc cattgtcggg ctgggagggc accgtcgacc aagtcctcaa catcatgtcc
     1981 cacgcctacc tcggtcgagg cctcgtcatc ccgacggtgc ccacccggga actgcgggaa
     2041 actgtcacct gattcctgtc gttcgggccg actccttttg atatgt
//
LOCUS       DASTXO010000136         2702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1039310, whole genome shotgun sequence.
ACCESSION   DASTXO010000136 DASTXO010000000
VERSION     DASTXO010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039310"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2702
                     /gene="dnaE"
                     /locus_tag="VFU96_02880"
     CDS             <1..>2702
                     /gene="dnaE"
                     /locus_tag="VFU96_02880"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU5112233.1"
                     /translation="LKTSEEMRRVFPLDTFPRACDNTLSIAERVDVDLDFGRILLPSF
                     PVPSGETEVSYLRRLVDQGARERYGHDARPEVWDRVEHELKIIEEMGFPAYFLIVWDL
                     IRHARESGIRVGPGRGSAAGSIVSYCLRITDLDPLAYGLIFERFLNPGRKEMPDIDMD
                     FDERYRAEMIRYAAQKYGPDRVAQIVTFSTIKGKQALRDAARVLGHPYGVGDRVAKAM
                     PPSILGREATLEQVMTPPESNAESTVKDWYANAQGLRDLYEADPAVRETVDAARGLEG
                     LRRQDSIHAAAVVIAPEPLINVVPIQQKGEGAEVVTQYEMYGVESLGLLKMDFLGLRN
                     LSIIERCLELVEAGTGQRLDIDAVPLDDEKTFQLLQSGNTVGVFQLEGNAMRSLIRSL
                     KPDSFNDVIALVALYRPGPMGANMHNLYADRKNGRAPIEELHPALTDKLADTYQIMVY
                     QEQVMSVAQEMAGYSMVDADNLRRAMGKKIKSVMKAEEEKFVAGCVAQGHEEKVGREI
                     FGLIEHFSGYGFNKSHSSGYGLVAYQTAYLKAHHPAEYLAALLTATKKDKDRTAVYLN
                     ECRQMGIEVLVPDVNESDTDFTVKDSRIRFGLSAVRNVGEGVVEKIVGARADGPFSSF
                     HDFVDRVDVSALNKRTVESLIKAGAFDAMGYPRKGLTVVYEQILDATLERRRNEDMGQ
                     FSLFAGDPGEVSHGRIEVPDLVWPQKIRLAFEKEMLGLYVSDHPLLSVGQSLAAATTT
                     TVSQLNDLTDKASVTVGGLVAGITRRWTRNGDPMIFFQLEDLEGSVEVVAFPRTVNEH
                     GHAIVEDAVVIVSGHLDHRGDEVKVMAREIKELEIRDDSMVRLKTAASSLTPEMVARL
                     KVILSNHPGPAPVYLHMISEQGVKVLKLSDAHRVEPRTALFAEL"
BASE COUNT          524 a          834 c          871 g          473 t
ORIGIN      
        1 tctgaagacc tccgaggaga tgcggcgtgt cttcccgcta gacacttttc cccgagcctg
       61 tgacaacacc ctctcgattg ccgagcgtgt cgacgtcgat ctcgactttg gcagaatcct
      121 cctccccagc ttcccggtcc cgtcgggcga gaccgaggtc agctatctgc ggaggttggt
      181 cgaccaggga gcacgtgagc gatatggcca cgatgccagg ccggaagtat gggaccgggt
      241 cgagcacgag ttgaagatca tcgaggagat gggctttccc gcctacttcc tcatcgtgtg
      301 ggacctgatc cgacatgctc gggagagcgg catcagggtg ggccctggtc gcgggtcggc
      361 agcgggctcg atcgtctcct attgcctgcg gatcaccgac ctcgaccctc tcgcctacgg
      421 attgatcttc gagcgcttcc tcaacccggg ccgcaaggag atgccggata tcgacatgga
      481 cttcgacgag cgataccgag ccgagatgat caggtatgcc gcccagaaat acgggcccga
      541 tcgggtcgcc cagatcgtca ctttctccac gatcaaaggg aagcaggcat tgcgtgacgc
      601 tgcccgggtc ctcggccacc cctacggggt cggtgaccgt gtggcgaagg cgatgccacc
      661 ctccatcctc ggaagggagg cgaccctcga gcaagtgatg acccctccgg aatccaatgc
      721 cgaatcgacg gtgaaggact ggtatgccaa cgcccagggc ctgcgggacc tgtacgaggc
      781 cgaccccgcg gtgagggaga ccgtggacgc cgcccggggg ttggaagggc tgcgtcggca
      841 ggactcgatc cacgcagctg ccgtcgtgat cgcccccgag ccactgatca acgtggtccc
      901 catccagcag aagggcgagg gcgccgaggt ggtgacccag tacgagatgt acggggtcga
      961 gtcactcgga ctgctcaaga tggacttcct cggcctgcgc aacctatcga ttatcgagcg
     1021 ctgcctggag ctggtggagg ccggcacagg gcagcgactc gacatcgacg cggtcccgct
     1081 cgacgacgag aagacgttcc aactcctgca gtcgggcaac acggtcggtg tcttccagct
     1141 cgaggggaac gcgatgcggt cgctcatccg ctcgctgaag ccggattcgt tcaacgacgt
     1201 catcgccctg gttgccctct atcgccccgg cccaatgggc gccaacatgc acaacctgta
     1261 cgcggatcgc aagaacggtc gggccccgat cgaggagctg catcccgccc tcaccgacaa
     1321 actggctgac acctatcaga tcatggtgta ccaggagcag gtgatgtccg tcgcccagga
     1381 gatggcgggg tattcgatgg ttgacgccga caacttgcgg cgagccatgg gcaagaagat
     1441 caaatcggtg atgaaggccg aggaggagaa gttcgtcgcg ggctgcgtcg cccagggcca
     1501 cgaggagaag gttgggcggg agatcttcgg gctgatcgag cacttctcag gctatgggtt
     1561 caacaagagc cacagctcgg gttacgggct cgtcgcctat cagaccgcat acctcaaggc
     1621 ccatcatccc gccgagtacc tggcggcgct gctgaccgcc accaagaagg acaaggaccg
     1681 aaccgccgtc tatctcaacg agtgccgaca gatgggcatc gaggtccttg tgcccgacgt
     1741 gaacgagtcg gacacggact tcaccgtcaa ggacagcagg atccgcttcg ggctgtcggc
     1801 ggtccggaac gtcggtgagg gggtggtgga gaagatcgtc ggggcgagag ccgacgggcc
     1861 attcagctcg ttccacgact tcgtcgaccg tgtcgacgtg tccgccctca acaagcgaac
     1921 ggtcgagtcc ctgatcaagg ccggggcctt cgatgccatg ggctacccga gaaagggcct
     1981 gactgtcgtc tacgaacaga tcctcgatgc caccttggag cgtcggcgca atgaggacat
     2041 gggccagttc tcgctgttcg cgggtgaccc aggggaggtc tcccacgggc ggatcgaggt
     2101 gcccgacctg gtctggccgc agaagatcag gctcgctttt gagaaggaga tgctcggtct
     2161 ctacgtctcc gatcacccgc tgctctcagt gggccagtcg ctggcggcgg ccaccaccac
     2221 caccgtcagc cagctcaacg acttgaccga caaggcgagt gtgaccgttg gtggtctcgt
     2281 ggcaggtatc acccgccgct ggacccggaa cggggatcca atgatcttct tccaactcga
     2341 ggatctggag ggatcggtcg aggttgtcgc cttcccccgg acggtcaacg agcatgggca
     2401 cgccatagtc gaagatgccg tggtgatcgt gagcggccat ctcgatcacc gcggcgacga
     2461 ggtgaaggtc atggcgcggg agatcaagga gctcgagatt cgtgacgact cgatggttcg
     2521 cctcaagacg gccgcctcga gcctgactcc ggagatggtg gcccggctca aagtcattct
     2581 ctccaatcac cccgggcccg ccccggtcta tctccacatg atcagcgagc aaggagtcaa
     2641 ggtgctcaaa ctgagcgatg cccatcgtgt cgagccacgg acggcgctct ttgccgagtt
     2701 ga
//
LOCUS       DASTXO010000137         2777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1702863, whole genome shotgun sequence.
ACCESSION   DASTXO010000137 DASTXO010000000
VERSION     DASTXO010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1702863"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="VFU96_02885"
     CDS             <1..1020
                     /locus_tag="VFU96_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112234.1"
                     /translation="IAAAGVTIGCNPPANTRFCPDDPIRRDQMATLLTRATHLTPNRP
                     PARCPTLPADDIWNTRVDGLPVHPRSAQYVSSIGSTAHLHPDFGSGVWPPGSNSPIGI
                     PYVEVQAGTTAVPVTYTAYGAESDPGPFPIPAGAPIEGGPDSTGDRHVIVVDRYRCEL
                     FELFDAHPGPGSSWRAASGARYQLDSSRLRPDGWTSADAAGLPIFPGLVRYDEVLSGH
                     IDHAIRFTAPLTRSAHVWPARHDASDSSNLALPPMGQRFRLSSEFDISGFAPQVRVIL
                     RAFKEYGIILADNGSSWYISGSPDPRWNNDVLRELKEIPGSAFEAVDVSSLIIDPNSG
                     QARQP"
     gene            complement(1043..1417)
                     /locus_tag="VFU96_02890"
     CDS             complement(1043..1417)
                     /locus_tag="VFU96_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112235.1"
                     /translation="MTNEKDTTSETIASRIGEALTGIFGEASPGTLFSEPEARGDDLI
                     LTAVAWERAGGFGFGAGQGQDEGESGSGEGGGGGGFSQGRPVAVIRITPNGVEVTPVI
                     DFTKIGVTLLLASVGVWRALSR"
     gene            complement(1407..1664)
                     /locus_tag="VFU96_02895"
     CDS             complement(1407..1664)
                     /locus_tag="VFU96_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112236.1"
                     /translation="MLRIRHTRREHVVDAADGVTLMVATSGFHWGTPFGGGGFTYHRP
                     VLVRHEGTAVVSPIRDHVMAVRLAGVAALLFALVWRWIDDK"
     gene            complement(1688..>2777)
                     /locus_tag="VFU96_02900"
     CDS             complement(1688..>2777)
                     /locus_tag="VFU96_02900"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEU5112237.1"
                     /translation="IPPIVVKIRLASLQETGSVVGTYSAIGTSGAIFGTFVTGFVLIA
                     AFPTRPIVIVVGVVLVLLGVALWVGRSAWTVVGSVVALLLISGLTLVFDGPCQYETTY
                     HCAEVLADTDRPSGRLLVLDRGHNSYVDLDDPTHLEFRYIRVMADLIDVEMPPGPLDV
                     VSIGGGGFTLPGYLAATRPGTDHRVLEIDASLVDIGRRDLGFEDEAEVIIDDARRSLT
                     RVPPGSVDVVVGDAFTGLTVPWHLTTLEFVEMIGDTLAPGGLYTVNVIDRPEARFARA
                     EAATLRQVFEHVAVFAPAGYFTGARGGNYVLVGSDEPIDMDRVQSALLSRGGVEAGLT
                     ADELTQFIDDARPLTDDFAPVDQMLSGL"
BASE COUNT          538 a          970 c          804 g          465 t
ORIGIN      
        1 atcgctgccg ccggagtgac catcggctgc aacccgccgg ccaacacccg gttctgcccc
       61 gacgacccga tccgaagaga ccagatggcc accctcctca cccgagccac ccacctcacc
      121 cccaaccgac cacccgcaag atgccccact ctcccagcgg acgacatctg gaacacccgt
      181 gtcgacggcc taccggtgca cccaagatcg gctcagtatg tgtcatcgat cgggtccacc
      241 gcccatctcc accccgattt cggctcgggt gtatggccgc ccgggtcgaa cagccccatt
      301 gggatcccct acgtcgaagt acaggccggt actacggcag tccccgtcac atataccgcg
      361 tatggcgccg agtccgaccc cggcccgttc ccgatccctg ccggcgctcc gatcgaaggc
      421 ggtccggatt cgacgggtga tcgtcatgtc atcgtggtcg accgatatcg atgcgagctc
      481 ttcgagctgt tcgatgccca tcccgggccc gggtcttcgt ggagagcagc atccggggcc
      541 cgatatcagc tcgactcatc tcgacttcgc cctgacggtt ggacgtcagc agatgccgcc
      601 ggactgccga tcttccccgg tctggttcga tacgacgaag tcctctctgg tcatatcgat
      661 cacgcgatca gattcactgc gcccctgacc agatcggcgc acgtgtggcc cgcccgacac
      721 gatgcctccg actcgtccaa cctcgccctt ccgccgatgg gccagagatt ccgactgtcg
      781 tcggagttcg acatctccgg gttcgcccca caggtgcgag tcattctgcg tgccttcaag
      841 gagtacggga tcatcctcgc cgacaacggc tcgtcgtggt acatatccgg cagccccgat
      901 cccagatgga acaacgacgt tctgcgagag ctgaaggaga tcccgggatc ggcattcgaa
      961 gccgtcgatg tctcatcctt gatcatcgat cccaattccg ggcaggcccg tcagccctag
     1021 ccaaccgggg gtcgcgatga cctcagcgac tgagagctcg ccagaccccg accgaggcca
     1081 gaagcagcgt gacgccgatc ttggtgaagt cgatcacagg tgtcacctcc accccattcg
     1141 gagtgatcct gatcaccgcc accggacgcc cttgcgagaa cccgccgcct cctccgccct
     1201 ctccgctccc gctctcgcct tcatcctgac cctggcccgc tccaaacccg aacccaccgg
     1261 cccgctccca agcgaccgcg gtcaggatca ggtcgtctcc gcgcgcttcc ggctcggaga
     1321 acaaggtgcc cggcgatgcc tcaccgaaaa tgccggtcaa cgcctcgccg atgcgtgaag
     1381 cgatcgtctc cgaagtcgtg tctttctcat ttgtcatcga tccacctcca gaccagggca
     1441 aacaggagag ccgcaacccc agccagtctg acggccatca catgatcccg aattgggctc
     1501 accaccgcgg tgccctcatg tctgaccaga acgggacggt gatacgtgaa tcctccaccg
     1561 ccgaacggtg tcccccagtg gaagccgctc gtggcgacca tcagggtgac accatcggcg
     1621 gcatcgacca cgtgctcgcg gcgagtgtgt ctgatccgca acatcggaca accctacaaa
     1681 ccggtcatca gagcccgctc agcatctggt cgacgggagc gaagtcgtcg gtcaaagggc
     1741 gggcgtcgtc gatgaattgg gtcagctcgt cagcagtcag gccggcttcg acaccacctc
     1801 tggagaggag cgccgattgg acacgatcca tgtcgatcgg ctcatccgat ccgaccagca
     1861 catagttgcc cccacgtgcg ccggtgaagt agcccgctgg agcgaacacc gccacgtgtt
     1921 cgaagacctg acgcagggtc gcggcctcgg cccgggcgaa cctagcctcg ggccggtcga
     1981 tcacattgac cgtgtagagc ccgcccggcg ccagggtgtc accgatcatc tcgacgaact
     2041 ccagggtggt caggtgccag ggcaccgtga gcccggtgaa ggcgtcaccg acgacgacat
     2101 cgaccgagcc gggcggcacc cgggtcaagg atctccgggc gtcgtcgatg atcacctcgg
     2161 cttcgtcttc gaacccgaga tcgcggcggc cgatatcgac aagcgaagca tcgatctcga
     2221 gcacccggtg atcggtgccc gggcgtgttg cggcgaggta tccgggcagc gtgaatcctc
     2281 ctccaccgat ggagacgaca tcgagagggc ccggcggcat ctccacatcg atcaggtcgg
     2341 ccatcacccg tatgtagcga aactcgaggt gggtgggatc gtcgagatcg acatagctgt
     2401 tgtgaccgcg gtccaggacc agcaaccttc ccgacgggcg gtcggtgtcg gctagcactt
     2461 cggcacagtg ataagtcgtc tcgtattgac acgggccgtc gaacacgagg gtcaacccgc
     2521 tgatgagaag cagtgccacg acagatccga caaccgtcca agcgcttcgg cccacccata
     2581 gggcaacacc caacagcacg agcaccactc cgacgactat cacgatgggc cgcgttggga
     2641 aggcggcgat caggacgaac ccggtgacga aagtgccgaa tatggccccg gaggtgccga
     2701 tggcggagta cgtgccgacc acggaaccgg tctcctgtaa cgaggcgagc ctgatcttga
     2761 ccacgatcgg tgggatc
//
LOCUS       DASTXO010000138         5813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1002460, whole genome shotgun sequence.
ACCESSION   DASTXO010000138 DASTXO010000000
VERSION     DASTXO010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5813
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002460"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VFU96_02905"
     CDS             complement(<1..213)
                     /locus_tag="VFU96_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112238.1"
                     /translation="MSELSAAAATALGIPEAIVQRSAAARAEETGMSVDDVLAAWAGG
                     GDIPPPPAAPAETEAAEEPAADEAAET"
     gene            complement(206..1657)
                     /locus_tag="VFU96_02910"
     CDS             complement(206..1657)
                     /locus_tag="VFU96_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEU5112239.1"
                     /translation="MAATQTADIRALLGAGDEDRVAVAHFLVGALFLVLAGVFQLGAL
                     FLLRFAGLSPISYGRVESMANITVMLGFLVLTLAGGIYYVLPRLTGARLWRKELAYLG
                     LLGVSGLVVVDLLAVVFGLGEGRQPFGLPWWLHLPMLAVLTIPFAVTMMTIRRREEKR
                     SFVTLWFVIGGTAWLPLLYLVHTAGTLPFVSSLGAEYSDLFFSAGFVTLWVFTVGSGL
                     FYYTLVKELDIPLASRQLASVGFWSLGFAAAWWGSAQLVFGPGPGWIDGVSAALGLAF
                     PIGALANAANASLTLQGHWEELRDKPGVVSGVVGLYLGVAVAILASFASFPSVGSVSA
                     LTAYWEAIEYAALYGVGVLLAAGVVFEALPRVAGREPATLDRPRSFNRWTVIGVGGVT
                     VSMIGAGLVSGYSWVAGSNSAAYVNAGEGWAAGAGSAEVLMLVAFGFGIVAFIGQLAY
                     AATVIGTVTAGRAVPQEVLVYGPDDSDEAEADE"
     gene            1824..2429
                     /locus_tag="VFU96_02915"
     CDS             1824..2429
                     /locus_tag="VFU96_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112240.1"
                     /translation="MYRLAELSDSRRKTIGWGLLALGFVGLVVAVLWIHYTSLPPEQD
                     KGIFNWIPSNHLLKGVGYLVVFGASQMLVVGALFVWVLNQTMTWARALFASFVTWIEL
                     VIIFGMVPSEWLNFAQTDLDWSSQRVAVTIPAILVLGNEVQLSWAAIKDSISMGYHLV
                     MLAAAAVFALQLQMMTKGPPASAEKPPPRSPYGRPLVRGEG"
     gene            2429..2878
                     /locus_tag="VFU96_02920"
     CDS             2429..2878
                     /locus_tag="VFU96_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEU5112241.1"
                     /translation="MPKGVVDETPPYRDDYQLAVVEGDYFTDKLRPKQFLHIDQSECI
                     LCEGCVDICPWKCIHYLSLDAITEGVNVDHPGEDPQNLGFFVVDEDECTRCALCVDRC
                     PTNVITLGKFEGSLAEQAAELGLDRRPWDFDTGQRDFKNGYAYGMRW"
     gene            2887..3663
                     /gene="extP"
                     /locus_tag="VFU96_02925"
     CDS             2887..3663
                     /gene="extP"
                     /locus_tag="VFU96_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenite/tellurite reduction operon b-type
                     cytochrome ExtP"
                     /protein_id="HEU5112242.1"
                     /translation="MANGNGKTGITDKIKEVNDKLWSSQVAESILRPGSPFKKGYSDS
                     PRNRSYVVMNNVLYHLHPVKVKRHGVKLSYTLCLGGLSFFLFILLTITGIFLMFYYTP
                     SAETAYADIEALSTNVAFGSLVRNMHRWGAHLMVLSVFLHMSRVFYHGAYKPPREFNW
                     VVGVLLLFLTLLLSFSGYLLPWDQLALWAVTVGTNMAGFVPVVGNEVKFALLAGVEVS
                     GATLLRFYVLHVLAFPFILVIFLAVHFWRVRKDGGISGPL"
     gene            3674..4816
                     /locus_tag="VFU96_02930"
     CDS             3674..4816
                     /locus_tag="VFU96_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112243.1"
                     /translation="MTEIPESLLRRSAEAKAKALGLPVEQVLAEMKGEAPPTPPPSAE
                     PAPAAEAGETVAEEMEEAAERSEEIEEAVESVDDEPAPAEEVAAEPAAETEPEVVAET
                     EADVPPAAPTETPPIENSEEALTPPASAQPSDDAGAETEVEPAAASVTTAVEERPAPE
                     GDGGGDGSGDGAGDGAGNGAGNGAGNGKVAAVVSARPTVPPKGTPEGVRTQRLLTVVK
                     ARAIQTVKAEPTDKVNTWPHLMLMEFGALLAITGLVVIMSVVIHSPLLDPANFNATPN
                     PSKAPWYFLGLQELLSYFDPQIAGVTIPTITGIIGFMMIPYVDKNPSTRPSDRKFAIA
                     LYSVFLAGAATLTIFGVLFRGLGFNFSYPWKDGVYFDDLKDWVQFE"
     gene            4813..5691
                     /locus_tag="VFU96_02935"
     CDS             4813..5691
                     /locus_tag="VFU96_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEU5112244.1"
                     /translation="MTLTNAALFAVAVVAILGAAGAFAVAYRRSQTTEKPDPLAGVSP
                     ETRAADRSATAVLIPPRVEEVEEAPEAKAPEAEPEPVEAEDDAVVDILETQRIVEVSP
                     AEGGVSRRQFFNRAIAATFGVFLAGQGLWYLAFFWPKLTGGFGADIDAGPVSDLRTQV
                     FTPDGAVLPLFIPEARAYLVPAPAELSDQFEGQGVETDGLMALYMRCVHLGCRVPWCA
                     PSQGFECPCHGSRYNAIGQYFGGPAPRNLDRFVVENQGGNLIIRTGTIIQTPRAPVLL
                     VPYPQGPSCISAVAQA"
BASE COUNT         1046 a         1912 c         1866 g          989 t
ORIGIN      
        1 cgtctcggcg gcttcgtccg ccgctggttc ctccgcggct tcggtctccg cgggcgcggc
       61 gggcggtgga gggatgtcgc cacctccggc ccaagcggcc agcacgtcgt cgacactcat
      121 cccggtctct tccgcccggg cggcggcgga gcgctgcacg atggcctcgg ggatgcccag
      181 ggcagtggcg gcagcagcgc tcagctcact catctgcttc cgcttcgtcg ctgtcgtccg
      241 gcccatagac caggacctcc tgcgggacgg ccctgcccgc ggtcaccgtt ccgatgacgg
      301 tggctgcata tgccagttgg ccgatgaagg ccacgatgcc gaagccaaag gcgacgagca
      361 tgaggacttc ggcggatcct gccccggcgg cccagccctc accggcgttc acataggccg
      421 cgctattcga ccccgcaacc cacgaatagc cactgaccag gccggctccg atcatgctca
      481 ccgtgacccc gcccacccca atgacggtcc agcggttgaa cgaccgcggc cggtccagcg
      541 tcgccggctc gcggccggcg acccggggca atgcctcgaa gacgacgccg gcagcaagga
      601 ggaccccgac gccgtacagc gccgcgtatt cgatcgcctc ccagtaggcg gtcagcgcag
      661 agaccgatcc gacggaaggg aaggaggcga agctggccaa gatggcaacg gccacaccca
      721 ggtagaggcc gacgacgccg gagaccactc ctggcttgtc cctcaattcc tcccaatgac
      781 cctggagggt gagggaggcg ttggccgcat tggccagggc cccgatgggg aaggccagac
      841 caagggcagc cgagaccccg tcgatccagc ccggccccgg gccaaagacg agttgggctg
      901 aaccccacca ggcggcggcg aaaccgagtg accagaaacc gaccgaggcg agctggcgtg
      961 aggcgagagg gatgtccagc tctttgacca gcgtgtaata gaagagccca ctgccaacgg
     1021 tgaacaccca gagggtgacg aaaccggcgg agaagaagag atcggaatat tccgcgccga
     1081 gggagctcac gaatggcagg gtgcccgccg tgtggacgag atagagcaag ggcagccagg
     1141 cggttccacc gatgacgaac cacagtgtga cgaagctccg cttctcctct cgacgccgga
     1201 tggtcatcat cgtcacggcg aaggggatgg tcagcaccgc cagcattggg aggtggagcc
     1261 accacggcaa gccgaaaggc tgccgacctt cgcccaaccc gaacacgacg gccaggaggt
     1321 cgaccactac gaggcccgac acgccgagga gcccgagata ggccagctcc ttgcgccaga
     1381 gccgggcacc ggtgagacgg gggaggacgt aatagatccc gcccgccagc gtcagcacca
     1441 ggaagccgag catgacggtg atgttggcca tcgactcgac ccggccgtag ctgatggggg
     1501 acagcccggc gaatctgagc aagaagaggg caccgagttg gaacacgccc gccaacacca
     1561 ggaaaagcgc acccaccagg aaatgagcga cggcgacgcg gtcctcatct ccggccccga
     1621 ggagggccct gatgtctgca gtttgggttg cggccaaggg ttccctcgtc gcggaacggg
     1681 acggcgggag cataactact tcctcgattc cttccgcagc catgaccgct ggcgcggaag
     1741 gccctcacca gggttctctc acgagacccg ggttgtggta gaaacgacgt gaacaggtat
     1801 acgaccgcgc ccgaagacga gagatgtatc gcctcgcaga actgagcgac agcagacgca
     1861 agaccatcgg atggggattg ctcgctctcg gcttcgtcgg tctggtggtg gcggtgctct
     1921 ggatccacta caccagcctc ccgcccgagc aggacaaagg catcttcaat tggataccga
     1981 gcaaccacct gctcaagggt gtcggttatc tggtcgtgtt cggggcctcc cagatgctgg
     2041 tggttggtgc cttgttcgtc tgggtgctca accagacgat gacctgggcc cgtgccctgt
     2101 tcgcttcctt tgtcacctgg atcgagctcg tcatcatctt cgggatggtc ccctcggagt
     2161 ggctcaactt tgctcaaacc gatctcgact ggtcctcgca acgggtggcg gtcaccatcc
     2221 ccgcgatcct ggtgctgggc aacgaggtcc agctgagctg ggccgcaatc aaggactcga
     2281 tctcgatggg ctatcacctg gtgatgctgg cggcggcggc ggtgttcgcg cttcagctgc
     2341 agatgatgac gaaaggccca cccgcctcgg ccgagaagcc cccgccccgc tcgccttacg
     2401 ggcgcccgtt ggtcagaggt gagggctgat gcccaagggt gtcgtcgacg agacgcctcc
     2461 gtatcgggac gattatcagt tggctgtcgt cgagggcgat tacttcaccg acaagctgcg
     2521 gccgaagcag tttctccaca tcgaccagtc ggaatgcatc ctctgcgagg gatgcgtcga
     2581 catctgccct tggaaatgca tccactacct gtcgctggat gccatcaccg aaggggtcaa
     2641 cgtcgaccat ccgggagagg acccgcagaa cctcggcttc ttcgtggtgg acgaggatga
     2701 atgcacccga tgcgcccttt gcgtcgaccg gtgcccgacg aacgtcatca cgttgggcaa
     2761 gttcgagggc tcacttgccg agcaggccgc cgagctcggc ctcgaccgtc gcccatggga
     2821 tttcgacacc gggcagcgtg acttcaagaa tggatacgcc tacgggatgc gctggtagga
     2881 ggcggattgg ccaacggcaa cggtaagacc ggcatcacgg acaagatcaa agaggtcaac
     2941 gacaagctct ggagctccca ggtcgccgag tcgatcctcc gtcccggctc gcctttcaag
     3001 aagggatact cggacagccc gaggaaccgg tcgtacgtgg tgatgaacaa cgtgctgtac
     3061 cacctccacc cggtgaaggt gaagcgacac ggcgtgaagc tgtcctacac gttgtgtctg
     3121 ggtggtctca gcttcttctt attcatcctc ctcaccatca ccggcatctt cctgatgttc
     3181 tattacacgc cttccgcgga gaccgcttac gccgatatcg aggccctgtc caccaatgtc
     3241 gcctttggct ccttggtgcg gaacatgcat cggtggggcg cccatctcat ggtgctctcc
     3301 gtcttcctcc acatgagccg tgtcttctat cacggtgcct acaagccgcc tcgggagttc
     3361 aactgggtgg tgggcgtgct gctcctgttc ctgaccctct tgctctcctt ctccggctat
     3421 ctgctgccct gggaccagtt ggcactgtgg gcggtcacgg tgggcaccaa catggcggga
     3481 ttcgtcccgg tagtcggcaa tgaggtgaag ttcgccctgt tggctggtgt cgaggtgtcc
     3541 ggtgccaccc ttctccgctt ctacgttctg cacgtcctcg ctttcccgtt catcctcgtc
     3601 atcttccttg cggtgcactt ctggagggtg cgcaaggacg gcggcatctc cgggcccctc
     3661 taaaggagtc gtcatgacag aaattccaga gagcctcctc aggagatcgg ccgaggccaa
     3721 ggccaaggca ttggggctgc ccgtcgagca ggtgctggcc gagatgaagg gggaagcccc
     3781 gcccacgcca ccgccctccg cggagcccgc gcccgcagcc gaggccgggg agacagttgc
     3841 cgaggagatg gaggaggctg ccgagcgctc cgaggagatc gaagaggcgg tggagtcagt
     3901 cgacgacgag cctgcgcccg cagaggaagt ggctgccgaa ccggcggccg agaccgagcc
     3961 agaggtcgtg gccgaaaccg aagccgacgt gccccccgct gcgcccaccg agacgccgcc
     4021 catcgagaac agtgaagagg cactgactcc tccggcctcg gcgcagccgt cggacgacgc
     4081 gggtgctgag acagaagtcg agcctgccgc cgcctcggtg actaccgcgg tcgaggaacg
     4141 gcccgccccg gaaggggacg gtggtggcga tggcagtggt gatggcgccg gcgacggcgc
     4201 ggggaacggc gccggcaacg gcgcggggaa cggcaaagtg gccgcagtgg tgagcgcccg
     4261 tccgaccgtg ccgccaaaag gaaccccgga gggggtccgg acccagcgtc tgctcactgt
     4321 ggtcaaggcc agggcgatcc agacggtcaa ggccgagccg acggacaagg tcaacacctg
     4381 gccccacctg atgctcatgg agttcggggc gttgctggcg atcaccggtc tggtggtgat
     4441 catgtcggtg gtcatccact ccccgctcct ggatcccgcc aacttcaacg ccactccaaa
     4501 tccctcgaaa gcgccgtggt acttcctcgg gctgcaggag cttctctcct acttcgatcc
     4561 ccagatcgcc ggggtgacca tccccaccat caccgggatc atcgggttca tgatgattcc
     4621 gtatgtcgac aagaaccctt ccacccgtcc cagtgaccgc aagttcgcca tcgccctcta
     4681 ctcggtgttc ctagccgggg cggcgacgct caccatcttc ggcgtcctct tcaggggcct
     4741 cgggttcaac ttctcctatc cctggaagga cggcgtctat ttcgatgact tgaaggactg
     4801 ggtgcaattc gaatgaccct gaccaacgcc gccctctttg cggtcgccgt cgtggcgatt
     4861 ctcggggcgg ccggggcttt tgcggttgcg tatcgccggt cccaaaccac cgaaaaaccc
     4921 gatcctctgg ccggcgtctc gccggagacc cgcgccgccg accgttcggc aaccgcggtc
     4981 ctgatcccac cccgggtcga ggaggtggag gaggctcccg aggccaaggc tcccgaggcc
     5041 gagcccgagc cggtcgaagc agaagacgat gcagtcgtcg atatcctcga gacccagcgc
     5101 atcgtcgagg tgtcgccggc ggaaggaggc gtcagcaggc gtcagttctt caaccgggcg
     5161 attgccgcca cctttggggt gttcctcgcc gggcaaggtc tctggtacct cgccttcttc
     5221 tggcccaagt tgaccggcgg cttcggcgcc gacatcgacg ccggcccggt gagcgacctc
     5281 cgcacccagg tcttcactcc cgatggcgcg gtgctcccgc tcttcatccc cgaggcccgg
     5341 gcttacctgg tccctgctcc cgccgagctg tccgaccagt tcgagggcca gggcgtggag
     5401 accgacggtc tgatggcgct ctacatgcga tgtgtccatc tcggctgccg ggtgccgtgg
     5461 tgcgcacctt cacagggatt cgagtgccct tgtcacggtt cgaggtacaa cgcgatcggc
     5521 cagtacttcg gtggcccggc accgcgcaac ctggaccggt tcgtggtgga gaaccaaggg
     5581 ggcaacctca tcattcgcac cggcacgatc atccaaacgc cccgggcgcc cgtgttgctc
     5641 gtcccgtatc cgcagggacc gagctgcatc agcgccgtgg ctcaggcatg accgccaata
     5701 tcatcactgt catcgccctg gtcgcaggga ttgcctggct gggcatgctg ttcgtgtccg
     5761 cgatccgcaa ccgcggcagc gaggaggtgg ctccgaatct ccggcccggg atc
//
LOCUS       DASTXO010000139         2385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1518494, whole genome shotgun sequence.
ACCESSION   DASTXO010000139 DASTXO010000000
VERSION     DASTXO010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518494"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VFU96_02940"
     CDS             <1..563
                     /locus_tag="VFU96_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016423138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5112245.1"
                     /translation="KEAIFADLDSVVAADSILASNTSSIPITRIAAATSRSDRVVGLH
                     FFNPVPVMGLVELVTTVMTSTETESATTAYVESLGKTVIKAQDRAGFIVNMLLVPYLI
                     GAIRMYEAGVASREDIDAGMRLGANHPMGPLELADFVGLDTMKHIADVLFDEYKDPFY
                     ASPPLLTRMVEAGLLGRKTGRGFYEY"
     gene            complement(571..>2385)
                     /locus_tag="VFU96_02945"
     CDS             complement(571..>2385)
                     /locus_tag="VFU96_02945"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU5112246.1"
                     /translation="LIRRRFGVALGVAVGGLVVPFVTGLALGFALPDSLLVDSADRTV
                     FALFIATALSISAMPVLAKILMDLDLMRREFGQTVLAAGMIDDLTGWTMLGLVTALAS
                     AEALTAGSVVLTVAMVAIFILATMTVGRVLVERGLGLIQDRFRGRDAILTLVVVLAFA
                     WGAFSQALRLEPVIGAFAIGILFGRLPRLPTDVVYKLESMALAVFAPIFFAVAGLKVD
                     IRSIFEPGLLFVAALVILIATFGKVAGAYVGARYFSGQGHWSALAYGAGLNARGAVEI
                     IIATIGLSLGILTTEMFSIIVLMAVVTSIAAPAGLRYCLARVEPGDEEELRLAREEVL
                     KGSFLAGLRRVLVPVRPRIGQVGTQTIQAVLVARLGEASDISTTLLAVAGDQDRVLAG
                     AYLNSLRAVFRDRGTTTRIVSSHDPVEAILHEAEADYDLLVIGTPTMSGTGEGVFGPV
                     IDDLIKLSRTPTLVVRGENVPEEWAPTRILVPASSSTSSRNAADLAFAIAGSDAVVTG
                     VHVVEPSRLSGMRPNVALDVTAEFEILATRLGQHVQTEVREADDIETGILDALVELDA
                     DLLVLGTSVRAGTTHLYLGPRVEYLTRLAPCPVVILNS"
BASE COUNT          466 a          760 c          771 g          388 t
ORIGIN      
        1 tcaaggaggc gatcttcgcc gatctggatt cagtggtggc ggccgactcg atcctcgcct
       61 ccaacacgtc atcgatcccg atcacgagga tcgctgcggc gacatccagg tccgacaggg
      121 tcgtcgggct tcatttcttc aatccggtcc cggtgatggg gctcgtcgaa ttggtgacga
      181 ccgtgatgac ctcgactgag accgaatccg cgacgacggc ttatgttgag tcgctcggga
      241 agaccgtgat caaggcgcag gatcgggcgg gattcatcgt gaacatgctt ctcgtcccct
      301 acctgatcgg tgcgatccgg atgtacgagg ccggcgtcgc cagccgtgag gacatcgacg
      361 ccgggatgcg actcggtgcc aaccatccca tgggcccgct cgagctcgcc gacttcgtcg
      421 gcctcgacac gatgaaacac atcgcagacg tcttgttcga tgagtacaag gaccccttct
      481 atgcctctcc gcccctgttg accagaatgg tggaagctgg tctgctcggg cggaagacgg
      541 gccggggatt ctacgagtac tgacagaccg ctagctgttc aggatcacga ccggacaggg
      601 cgccaagcgg gtcagatact ccactcgggg gccgagatag aggtgggtgg tgccggcacg
      661 cacgctggtg ccaaggacca gcaggtcggc atcgagttcg accagggcgt cgaggatgcc
      721 tgtctcgatg tcgtcggcct ctctgacctc ggtttggacg tgttgtccca ggcgtgtggc
      781 gaggatctcg aattcagcgg tcacgtcgag cgcgacgttt ggtcgcatcc cggacaggcg
      841 agatggctcc acgacgtgca ccccggtcac cacggcatcc gatccggcga tggcaaaggc
      901 gaggtcagcc gcatttcggg agctggtgct ggagctggcc ggcaccagga tccgggtggg
      961 ggcccattcc tcggggacgt tctcccctct caccacgagg gtgggagtac gggacagctt
     1021 gatcaagtcg tcgatgaccg gcccgaacac accttcgccc gtgccactca tcgttggggt
     1081 tccgatcacg agcaggtcat agtcggcctc ggcctcgtgc aggattgcct ccaccggatc
     1141 gtggctggac acgatgcgcg tggtcgtgcc tcggtccctg aagacggcgc ggagcgaatt
     1201 gaggtaggcg cctgccagga cgcggtcttg atccccggcg acggcgagaa gtgtggtcga
     1261 gatgtcgctg gcctcaccga ggcgggccac caacaccgcc tggatagttt gcgtgcccac
     1321 ctgaccgatc ctcggtctga ccggcaccag tacccgtctc aaaccggcga ggaagctgcc
     1381 cttgaggact tcctcgcggg ccaatctcaa ctcctcctcg tcgccaggct cgacccgggc
     1441 caggcagtac cgaagccctg cgggcgcggc gatagaggtc accacggcca tcaacacgat
     1501 gatggagaac atctcggtgg tcaggatccc gagagacaga ccgattgtgg cgatgatgat
     1561 ctccaccgcc ccacgggcat tgagaccggc gccgtaagcc agggctgacc agtgtccttg
     1621 gcctgagaaa tacctggctc cgacataggc gccggcgacc ttgccgaacg tggcgatgag
     1681 gatcacgagt gcggcgacaa acagcagacc gggctcgaag atcgagcgaa tgtccacctt
     1741 caagccggcc accgcgaaga agatcggagc gaagacggcc agggccatgc tctccaactt
     1801 gtagacgacg tcggtgggaa gccggggcag ccggccgaac aggatcccga tggcaaaggc
     1861 cccgatgacc ggctcgagtc tgagcgcctg gctgaaagcg ccccaggcaa aggcaagcac
     1921 cacgacgaga gtcaggatgg cgtcgcggcc ccggaatcgg tcctggatca agcccagccc
     1981 ccgctcgacg aggactctcc cgacggtcat cgtggccagg atgaagatgg ccaccatcgc
     2041 gacggtcagc accaccgaac ctgcggtcag ggcctcagca ctggccaatg ccgtgaccag
     2101 gcccagcatc gtccagccgg tgaggtcatc gatcattccg gcagcaagga ccgtctggcc
     2161 aaactcccgc cgcatgaggt cgaggtccat caggatcttc gccagtaccg gcatcgccga
     2221 gatcgacagc gccgtcgcga tgaatagcgc gaacacggtt cgatccgccg agtcgaccag
     2281 caggctgtcg ggcagggcga agcccagcgc gagcccggtg acaaacggca ctacgagacc
     2341 gcccaccgcc actccgagag cgactccaaa gcggcgccgg atcag
//
LOCUS       DASTXO010000140         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1739333, whole genome shotgun sequence.
ACCESSION   DASTXO010000140 DASTXO010000000
VERSION     DASTXO010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1739333"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFU96_02950"
     CDS             complement(<1..234)
                     /locus_tag="VFU96_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020130908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5112247.1"
                     /translation="MPKVLVVDDELKIARLVRDYLTEAGFDVALASTGPAAVASVRSD
                     RPDLVVLDLGLPGMDGYDVTRAIRGQSAIPIIML"
     gene            complement(263..661)
                     /locus_tag="VFU96_02955"
     CDS             complement(263..661)
                     /locus_tag="VFU96_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112248.1"
                     /translation="MRRNVIGTVLITLLVVGALGAIGFGLWQMGYQRGLVETGAEVVV
                     NTGRAMQGWWGWGFGGFGFFGFFFKILFFFLLIGLIARLFFGPRRWGPYWGRDWHEGQ
                     SSPMEQHMTEWHDRAHGSTPNPKPDPERDA"
     gene            810..2048
                     /locus_tag="VFU96_02960"
     CDS             810..2048
                     /locus_tag="VFU96_02960"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEU5112249.1"
                     /translation="MTAPSRFRLWQPPARFEDRPSERRISFLELFFDLVFVVVVAQIS
                     HRLSEHPTWAGVGWFVALFFAVWSSWTNGPLYYDLHGTNDVSVRVFTFAQMLAVALMG
                     VFIGSVPGDGEAGFALAYAANVLVLVFIWFRTGVHDSTHRAASHPYSGFYLLSALTFA
                     GSVWVPPPGRYWLWALGLALQIIGQIVAFRRWTPPESQGGEAVIAVTGSLVERLDLFV
                     IIVLGEVIVGSVTGMAGVEVLDLDAAAAGMLGVLVAIGLWWIYFDLVSLREPISRYSQ
                     LWLNLHFPLVVAITAGGAGVLNTVERPSGDLPDEVRWLLVGAMAVAIVSVALLTRTLQ
                     ARAQGSEIYLRAELTMYASAVLALAVGLTDWGANPTLMAMVILLLAPVFVALHVLKQR
                     LAAARAATLKPVPTPRSQTS"
     gene            complement(2011..>2299)
                     /locus_tag="VFU96_02965"
     CDS             complement(2011..>2299)
                     /locus_tag="VFU96_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112250.1"
                     /translation="DAAFGTDCDAWYPLTPLDPLMTTIPEGKWVLGTHITPGTYQTPG
                     GEGCYWERLSGVSGTFEDIIANGTPAGQAVVEIDPSDIAFNSFGCGEWAPA"
BASE COUNT          370 a          744 c          746 g          439 t
ORIGIN      
        1 gagcatgatg atcggtattg ccgactggcc ccggatcgcc cgggtcacgt cataaccgtc
       61 catcccgggg aggcccaggt cgaggaccac caggtcggga cgatccgagc ggaccgaggc
      121 caccgccgcc ggccccgtcg aggcgagggc gacgtcgaag cccgcctcgg tcaggtagtc
      181 cctgacgagg cgggcaatct tcagctcgtc gtcgacgacg aggaccttgg gcacgccggt
      241 cattgtcgcc gccggacgtg agctaggcgt cccgttcggg gtcgggcttc ggattcggcg
      301 tgctgccgtg agcccggtcg tgccattcgg tcatgtgctg ctccatcggg gagctctggc
      361 cttcgtgcca gtcgcgaccc cagtacggcc cccaccggcg cggcccgaag aacagccggg
      421 cgatcaggcc gatcagcaga aagaagaaca ggatcttgaa gaagaacccg aagaacccga
      481 atccgccgaa gccccagccc caccagccct gcatcgcacg cccggtgttg accaccactt
      541 ccgctccggt ctccacgagc ccccgctgat agcccatctg ccaaagaccg aagccaatcg
      601 cgccgagcgc ccccacgacg agcagagtga tgagcaccgt gccgatcaca ttccttctca
      661 tgtctcagaa ctcctttcca agagactcga cgtcaggatc ggtcctgaat gtggagacga
      721 tgtgaagaag aagtggaggt tcagccgacg gactcgtcca gggttcggcg caaacagtca
      781 tgcaatgagg atccttaaca ttcctcacca tgaccgcgcc gtcgaggttc cgtctctggc
      841 agcccccggc ccgattcgaa gatcggccca gcgaacgtcg gatcagcttc ctcgagctct
      901 tcttcgacct ggtcttcgtg gtggtcgtgg cccagatatc gcatcggctg tccgagcacc
      961 cgacgtgggc cggcgtgggt tggttcgtgg ccttgttctt cgcggtgtgg tcgtcatgga
     1021 ccaacgggcc cctctattac gacctgcacg ggaccaacga tgtcagtgtc cgagttttca
     1081 ccttcgccca gatgctggct gtggcgttga tgggtgtctt catcggcagc gtcccgggcg
     1141 atggcgaggc cggctttgcc ctggcctatg cggcgaacgt cctcgtcctc gtcttcatct
     1201 ggtttcggac cggggtccac gattccactc accgggcggc ctcgcatccg tattcaggct
     1261 tctatttgct atcggccttg acattcgccg ggagcgtgtg ggtaccgcca ccggggcgtt
     1321 actggctgtg ggcgttgggt ctggcgctcc agatcatcgg ccagatcgtg gcgttccgtc
     1381 gctggacccc gccggagagc cagggaggag aggcggtcat cgccgtcact ggctcattgg
     1441 tggagcgtct cgacctgttc gtgatcatcg tcctcggaga ggtgatcgtt ggctccgtca
     1501 ccggcatggc cggggttgag gtgctcgatc tggacgcggc cgcggccggg atgctggggg
     1561 tgctggtcgc catcgggttg tggtggatct acttcgatct ggtctcgctg cgcgagccga
     1621 tctcccgata cagccagttg tggctgaatc tgcatttccc tctagtcgtc gccataaccg
     1681 ccgggggagc cggtgtgctc aacaccgtgg aaagaccgtc cggggatctg cccgatgagg
     1741 tgcgatggct gctggtgggc gccatggcgg tcgccatcgt gtcggtggcg ctcctcaccc
     1801 gaaccctgca ggcccgagcc cagggctcgg agatatatct ccgggccgag ctcaccatgt
     1861 acgccagcgc tgtcctcgcg ctggcggtgg ggctgacgga ttggggggcc aacccgacgc
     1921 tgatggcgat ggtgattctc ctgctcgccc ccgtgttcgt ggcccttcac gtgttgaaac
     1981 agcgcctggc cgcggcacga gcggcaacac tcaagccggt gcccactccc cgcagccaaa
     2041 cgagttgaac gcgatgtcgg agggatcgat ctccaccacc gcctgaccgg caggggtgcc
     2101 attggcaatg atgtcctcga atgttcccga gactccggag agacgctccc aatagcagcc
     2161 ctcgccgccg ggcgtctggt aagtacccgg cgtgatgtgc gtccccagga cccactttcc
     2221 ctccgggatc gtggtcatca gcgggtcgag cggggtgagc gggtaccagg cgtcgcaatc
     2281 ggtcccgaag gcggcatcg
//
LOCUS       DASTXO010000141         3309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_892660, whole genome shotgun sequence.
ACCESSION   DASTXO010000141 DASTXO010000000
VERSION     DASTXO010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892660"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VFU96_02970"
     CDS             <1..387
                     /locus_tag="VFU96_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112251.1"
                     /translation="LHGRVDLLNFELRRRSGQEERTLIEALPEILASGMTFGKEPNLR
                     HIDTMPPLPASTGRRLIDRIMDDGILTQLPELTDDELTEAIDRVQEVEAELSAQRRQL
                     HVVIDALQDEIIARYRSQQGEAQPAG"
     gene            391..1344
                     /locus_tag="VFU96_02975"
     CDS             391..1344
                     /locus_tag="VFU96_02975"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEU5112252.1"
                     /translation="MLAASTRSGLVETMHDGAVAVVTPNGDLVAHSGDIDQPFYLRSA
                     AKPFQALASQEHGAALGPLELALACASHDGHPVHVGLVESMLARAGLSEPDLQCPPSW
                     PLSTAAANRLRIEMGGDGEPRAIWHDCSGKHAGWLRACHAQGWPLDRYLAPEHPIQVA
                     ITDTINDLGGFPASPVGVDGCGAPVHRTTTRAMATLYARLGSLPRLREVFDTMHRYPA
                     LVSGTGNGDASIATALNAAAKRGAAGCIGVAIDRRLGLAVKSWDGIQEVADAAAIATL
                     SGLGELSRVAADRLAALARPTVLGGGRPVGELEPRVELTWS"
     gene            1341..1760
                     /locus_tag="VFU96_02980"
     CDS             1341..1760
                     /locus_tag="VFU96_02980"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEU5112253.1"
                     /translation="MREVLELRIERWLSDPDSSVEYAEEMEGRWAVRMRQEARDATTV
                     WFTIGQRSIEYEAYVMPAAEGGRELFEQALHRNMRGWRAHYALDQEGGLLLRGRLAEP
                     NVTDLELDLALGEIYDAIEVGFRPLLRSLIAGRENSG"
     gene            2071..2928
                     /locus_tag="VFU96_02985"
     CDS             2071..2928
                     /locus_tag="VFU96_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5112254.1"
                     /translation="MAGLQKKNLDKPDERVQFDGLEADIVQVGEASISRNVFQPGAHC
                     ALGGRRLSGNHRAKESCQAHHSGVVVQGRLRIEMDDGSAMDLGPNEVFDIPPGHDGWV
                     VSEEPWVGINWSGVRTWLPQVESGDRVLATLLFTDIVSSTETAVQLGDRAWRDLLGRH
                     NQEVRDQLDRFRGREVATTGDGFLAVFDGAARAIHAAQAIRDRARGLGLEIRSGIHTG
                     EVEVVGDDVRGVAVHEAARIAAAASPGEILVSSTTHDLAAGGEFTFEETPGRELKGLS
                     GARILHTVV"
     gene            complement(2945..>3309)
                     /locus_tag="VFU96_02990"
     CDS             complement(2945..>3309)
                     /locus_tag="VFU96_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112255.1"
                     /translation="SGHAANILGDYNYVGVGTDTSSDGRLYVTVVFMKKGETAPPTTE
                     VPPTTEAPPPDSPDTTAPSPDEGGEPAAPASTTTTPVTTTTTAPTTTTTMVVPPDHAV
                     IPGQSCIEASRFAQLCHD"
BASE COUNT          557 a         1027 c         1144 g          581 t
ORIGIN      
        1 cttcatggtc gggtcgatct gctcaatttc gagttgcgcc gccgcagcgg gcaggaggaa
       61 aggaccctga tcgaggcctt gcctgagatc ctcgcctcgg ggatgacctt tggcaaagag
      121 ccaaatctgc ggcatatcga cacgatgccg ccgctgccgg cttcgaccgg ccgccgcctg
      181 atcgaccgga tcatggacga cggcatcctc acccagctcc ccgagctgac cgacgatgag
      241 ctcaccgagg cgatcgatcg tgtgcaagag gtggaggccg agctctcggc ccagcggcgc
      301 cagctgcatg tcgtcatcga cgccctgcag gacgagatca tcgcccgcta caggagccag
      361 cagggcgaag cacagccagc aggctgaagg ttgctcgcgg ccagcacacg ctccggcctg
      421 gtcgagacca tgcacgacgg ggcggtggct gtcgtcacgc ccaacggcga cctggtagcc
      481 cactccggcg acatcgacca gcccttctac ctccgctcgg cggccaagcc gtttcaagca
      541 cttgcctcgc aggaacacgg cgccgcattg ggcccgctcg agctggccct ggcctgcgct
      601 tcgcatgacg ggcatccggt gcatgtcggc ctcgtggagt cgatgctggc gagggccggg
      661 ctcagcgagc ccgatctcca gtgcccaccc agctggccgc tgtcgactgc cgcggcgaac
      721 cgtctcagga tcgagatggg tggagacggc gagccacgag ccatctggca cgactgctcg
      781 ggcaagcatg cgggatggct ccgagcctgc catgcccagg gctggccgct agatcgctac
      841 ctggccccag agcatccaat ccaggttgcc atcaccgaca cgatcaacga tctgggtggc
      901 ttcccggcct cgccggtggg tgtcgacggt tgcggggcac cggttcatcg caccacgacc
      961 cgggccatgg ccaccctcta tgcccgtctc ggctctcttc cacggctcag ggaggtattc
     1021 gacaccatgc accgttatcc ggccctggtc tcgggcaccg gtaacggcga tgcgtcgata
     1081 gccaccgccc tgaacgcagc cgccaagcgc ggtgctgcgg gatgcatcgg tgtggccatc
     1141 gaccgacgtc tcggtctggc ggtgaagtcc tgggacggga tccaggaagt ggccgatgcc
     1201 gctgccatcg caacgctgtc ggggctgggt gagttgtccc gagtcgcagc cgatcgcctc
     1261 gccgcactcg ctcgaccgac cgtgctcggt ggagggcggc ccgtcggaga gctcgagccc
     1321 cgggtggagc tgacttggtc atgagagaag tgctcgagct ccggatcgag cggtggctct
     1381 ccgaccccga ctcgagcgtg gaatacgccg aggagatgga gggccgctgg gccgtgcgga
     1441 tgcggcagga ggcacgcgat gccaccaccg tctggttcac gatcgggcaa cgaagcatcg
     1501 agtacgaggc ctatgtcatg cccgcggccg agggcggccg tgagctcttc gagcaggcgt
     1561 tgcatcggaa catgagaggt tggagggcgc actacgccct cgatcaagag ggcgggctgt
     1621 tgctacgcgg ccggctcgcc gagccgaacg tcaccgacct cgagctcgac ctggcgctgg
     1681 gcgagatcta tgacgcgatc gaggtcggct ttcggccatt gcttcggtcc ctgatcgcag
     1741 gtcgcgaaaa ttccggttag aaacttgtga acaaacccat gtggacaaag cctgtggaaa
     1801 gccgggattc ctgttggtaa ccctggtcaa gaccgagcgg gcatcttcgt tggcgatgga
     1861 gagcgttact cggcgatgga tgacaaccga gtcgaagtcg ttgcttccgg tcggcagaac
     1921 acgtacattc gccgcagcga acgcccgaga ggcaaaccag agaaccgtcc gttcggggaa
     1981 gtgcggagga ggctcaggtg ggtcactcgg gcgttctctg ttgccggccc gccggatgtc
     2041 accacggctc gattcggtac gatcaggcac ttggccggcc tccagaagaa gaacctcgac
     2101 aaaccggacg agcgggtcca gttcgatggg ctcgaggccg acatcgtgca ggttggcgag
     2161 gcctcgatct cccggaatgt gttccaaccc ggtgctcact gtgccctcgg tgggcgccgc
     2221 ctgagcggca accatcgagc gaaggagagt tgtcaggccc atcattcggg tgtcgtcgtg
     2281 cagggccggt tgcgaatcga gatggatgac ggctcggcca tggacctggg gcccaacgag
     2341 gtgttcgaca tacctccggg ccatgatggc tgggtagtga gcgaagagcc ctgggtcgga
     2401 atcaactggt ccggcgtccg cacctggctt cctcaagtcg agtctggcga tcgcgtcctg
     2461 gccacgcttc tgttcaccga tatcgtgtcc tccaccgaga cggcggttca gctgggggac
     2521 cgagcctggc gtgatctcct gggccggcac aatcaggagg tcagagatca gctcgatcgg
     2581 tttcgaggac gtgaggtggc cacgaccgga gacgggtttc tggccgtctt cgacggggcg
     2641 gcaagggcca tccatgccgc ccaggccata cgtgataggg cccgtggact cggtctggag
     2701 atccgatccg ggatccacac cggtgaggtc gaggtggttg gtgacgatgt cagaggagtc
     2761 gcggtacatg aagcggcccg gatcgcagcg gcggcgtcac cgggcgagat ccttgtctct
     2821 tctacgaccc acgacctggc tgccggtggc gagttcacct tcgaggagac accgggtcga
     2881 gagctcaagg gcttgtccgg agcgagaatc ttgcacaccg tcgtgtagca agcctctgat
     2941 cggatcagtc gtgacagagc tgggcgaacc ggctggcctc gatgcacgac tgacccggga
     3001 tcaccgcatg gtctggtggc acgaccatcg tcgttgtcgt cgttggcgcc gtggtcgtgg
     3061 tggtcacggg ggtggtggtg gtcgatgccg gcgccgccgg ctcgccgccc tcgtccggtg
     3121 atggcgccgt ggtgtctggt gagtcgggtg ggggggcctc ggtggtcgga ggcacctcgg
     3181 tcgtcggcgg ggccgtctcg cccttcttca tgaacacgac ggtgacatag agcctcccat
     3241 cgctgctggt gtcggtgccg acaccgacgt agttgtagtc gccgaggatg ttggcggcgt
     3301 ggcctgagg
//
LOCUS       DASTXO010000142         2018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_856242, whole genome shotgun sequence.
ACCESSION   DASTXO010000142 DASTXO010000000
VERSION     DASTXO010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856242"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VFU96_02995"
     CDS             <1..729
                     /locus_tag="VFU96_02995"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU5112256.1"
                     /translation="FHEVGEGRRPIINISGGTEIGAVIVGVNILQGLKPTSVGGPSFG
                     IDADIYDAEGNPVRGEVGELVIRGSWPGMTRGFWGEPERYLETYWSRFPDVWVHGDWA
                     SIDDDGFWFLHGRSDDTLNIAGKRVGPAEIESAVVALPEVVMAAAIGIPDEVKGESIA
                     LYVVPSPDVEPDDQLTGKVLTAVTETMGNAFRPKSVLWVADLPRTRSAKIMRRVIKAV
                     ALGDEPGDLSGLENPESLEGLRIL"
     gene            complement(763..1758)
                     /locus_tag="VFU96_03000"
     CDS             complement(763..1758)
                     /locus_tag="VFU96_03000"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007072917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEU5112257.1"
                     /translation="MPEPILSVQDLVKHYPIRRGVFRRQVGSIKAVDGVSFDLMPGET
                     LGLVGESGCGKSTVARTLLRLVQPTSGSAYYKGTDIFSLDKGDLRELRRHIQIIFQDP
                     YASLNPRMTVGDIVAEPWAIHSGLLPKNQVRDRVRQLLEFVGLNPNHINRYPHQFSGG
                     QRQRIGVARALALNPSIIICDEPVSALDVSVQAQVVNLLEDIQDEFNLSYIFIAHDLS
                     IVRHISDRVAVMYLGKIVEEGVESDLYGNPSHPYTQALLSAVPVPDPDFHSDKRILLQ
                     GDVPSAANPPSGCHFRTRCWKAQDICAEVEPALEQHFDTLAACHFAAPREVFATD"
     gene            complement(1751..>2018)
                     /locus_tag="VFU96_03005"
     CDS             complement(1751..>2018)
                     /locus_tag="VFU96_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020556612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEU5112258.1"
                     /translation="YRRPYHPYTEGLMRSLVRPDHKGGRLSPIKGSPPDLLHIPSGCP
                     FHPRCDYVQDICRTDDPPLRVIEVDRTSACHFAEDFMAGELADA"
BASE COUNT          371 a          613 c          687 g          347 t
ORIGIN      
        1 ttccacgagg tcggcgaggg gcggaggccg atcatcaaca tctcgggcgg gaccgagatc
       61 ggcgccgtca tcgtcggtgt caacatcctt caggggctca agccgacctc cgtgggcggc
      121 ccttccttcg ggatcgacgc cgatatctac gacgctgagg gcaacccggt gcggggcgaa
      181 gtgggcgagt tggtgatcag gggatcctgg ccgggcatga cccgcggttt ttggggcgag
      241 ccggagcgct acctcgagac ctactggtcc cggttccccg acgtctgggt gcacggtgat
      301 tgggcgtcca tcgacgacga cggcttctgg ttcctccacg gccgttcgga tgacacactc
      361 aacatcgcgg ggaaacgggt cggcccggcc gagatcgagt ccgcggtggt ggccctgccc
      421 gaggtggtga tggcagcggc gatcgggatt cccgacgagg tcaagggaga gtcgattgcc
      481 ctctacgtcg tgccctcccc tgatgtcgag ccggacgacc agctgacagg gaaagtcctc
      541 acggccgtga cagagaccat gggcaacgcc ttccgcccga agagcgtcct ttgggtggcc
      601 gacctgcccc gcaccaggtc ggcgaagatc atgcggcggg tcatcaaggc cgtcgctctg
      661 ggagacgagc cgggcgatct atccggtctg gagaacccgg agagcctcga agggctcaga
      721 atcctctgag gcggggcggg gcagccccgg cccaccggtc attcagtccg tcgcgaagac
      781 ctcgcggggt gcggcgaagt ggcaagcggc gagggtgtcg aaatgctgct ccagcgccgg
      841 ctcgacctcg gcgcagatgt cctgggcttt ccagcaacgg gtgcggaaat ggcatccgga
      901 tggcggattc gccgccgacg gcacatctcc ttgaaggagg attcgcttgt cggagtggaa
      961 atccggatcc ggcaccggca cggccgagag cagggcctga gtgtatgggt gggacgggtt
     1021 gccatacagg tccgactcca ccccctcctc gacgatcttt cccagataca tcacggccac
     1081 ccggtccgag atgtgacgga ctatggagag atcgtgggca atgaagatat aggacaggtt
     1141 gaactcgtcc tggatgtcct cgagcaggtt gaccacctgg gcctgcactg agacgtcgag
     1201 ggccgacacc ggctcgtcac aaatgatgat gctcggattg agggcgaggg cccgcgccac
     1261 cccgatccgc tgcctctgcc cacccgagaa ctggtgtggg taacgattga tgtgatttgg
     1321 gttcagtccg acgaactcga gtaactggcg aacccggtct ctcacctgat tcttgggtag
     1381 gaggccgctg tgaatcgccc atggctcggc gacgatgtcc cccactgtca tcctcgggtt
     1441 gagcgaggcg tacgggtcct ggaagatgat ctggatgtga cgacgcagct cgcggaggtc
     1501 gcccttgtcc aacgagaaga tgtcggtgcc cttgtagtag gccgatcccg acgttggctg
     1561 caccagacgg agcagggtgc gggccacggt cgacttcccg catccggact caccgaccag
     1621 ccccagcgtc tcacccggca tgaggtcgaa actcaccccg tcgacggcct tgatcgaacc
     1681 gacctgacgg cggaacaccc ctctgcggat cgggtagtgc ttgaccaggt cctgaacgga
     1741 gagaatcggc tcaggcatcg gccaactccc cggccatgaa atcctcggca aagtgacacg
     1801 ctgaggtccg atccacctcg atcacccgca gcggcgggtc atccgtccgg cagatgtcct
     1861 ggacatagtc gcaacgagga tgaaaggggc accccgaagg gatgtggagg aggtctggtg
     1921 gcgatccctt gatcggggag agccggccac ccttgtgatc ggggcggacc agggagcgca
     1981 tcagaccctc ggtgtagggg tgatagggcc gcctatac
//
LOCUS       DASTXO010000143         9830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_450639, whole genome shotgun sequence.
ACCESSION   DASTXO010000143 DASTXO010000000
VERSION     DASTXO010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 9830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9830
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450639"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /gene="aceE"
                     /locus_tag="VFU96_03010"
     CDS             <1..307
                     /gene="aceE"
                     /locus_tag="VFU96_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020521423.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEU5112259.1"
                     /translation="VTRLLSDVGGPIVAVTDYMRLVPDQVSRWVPGPLTTLGTDGFGR
                     SDARKALRRFFEVDAGHIVAATLSTLAGQGEMPETAVRDAFDRYGIDPEAPDPAISR"
     gene            307..1485
                     /gene="moeB"
                     /locus_tag="VFU96_03015"
     CDS             307..1485
                     /gene="moeB"
                     /locus_tag="VFU96_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEU5112260.1"
                     /translation="MISYRDLVGRARAQIEEMTPEDLEPRLAEVLVIDVRETGEFEQG
                     SIPGARLLPRGLLERDITGVAPDRSQPVVVYCASGGRSALAAVSLQEMGYETVYSLAG
                     GFDRWKSEGKAWGAGEGLTAEQRDRYSRHVILEEVGEAGQARLLDSSILLIGAGGLGS
                     PAALYLAAAGVGTIGIVDYDVVDRTNLQRQVLHNLDRVGMPKTESAREAITALNPDVK
                     VVTYNERLDAENALDIMKGYDVVLDGGDNFPTRYLVNDASLHLRVPVVHGSIFRFEGQ
                     VSVFDPYAGPCYRCLFPEPPPPELAPSCAEAGVLGVLPGIIGSIEAMEAIKLLLDLGE
                     PLTGRLLIYDALEEDFNSLRIDRDPDCPACADPDRPPRIVEYDQTCRPGGNVERAPAS
                     "
     gene            1469..1849
                     /locus_tag="VFU96_03020"
     CDS             1469..1849
                     /locus_tag="VFU96_03020"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEU5112261.1"
                     /translation="MPQLPDQIRWAILAHAENCVPNECCGLLAIDREGRFRFAYPLSN
                     ADPSPVSYTIDPDEHFQAMRHAESMGLEIAGVFHSHPGGTAMPSMIDVQSALEPDWMY
                     LIAAPGEIRGFYIRNGVIEEIALD"
     gene            complement(1852..2268)
                     /locus_tag="VFU96_03025"
     CDS             complement(1852..2268)
                     /locus_tag="VFU96_03025"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HEU5112262.1"
                     /translation="MIFDFFGELNWLAIVVATIAWFAFSAIWYSVPPLSKAWQKASKV
                     DMTTEGPPLASLFGPTLVGYFVTTVVIALIARGIGATEVVDGVALGAALGVGFGAVSA
                     LVSQIYEQKGSSYWLINGLNAIIAYIIVALIVTVWV"
     gene            complement(2340..3233)
                     /locus_tag="VFU96_03030"
     CDS             complement(2340..3233)
                     /locus_tag="VFU96_03030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5112263.1"
                     /translation="MTLPQGLVTRPLAPGDESVVTAVIAAGQLEDVGEVVIEEADVVS
                     DWQRPSYDVGASTIGIFDDDRLIAYAELSGGDRGDAAVDPTYRGRGIGTFLANWMQGQ
                     ARARGLQVIGMPVPRGSSGDRLLERLGYRIRWTSWALALPEGQVIEAPPLPPGYTIRT
                     AELADHRAVWTVIEDAFLEWSVRDRQSFEDFSAQVLHRPGFEPWNLRVVTDGIGDVVG
                     AVLILLADEVGFVERIAVRKDQRGMGLGQALLADAFARAREHGATRSELSTDSRTGAL
                     GLYERVGMEVTSVWVNRAIDL"
     gene            complement(3300..3551)
                     /locus_tag="VFU96_03035"
     CDS             complement(3300..3551)
                     /locus_tag="VFU96_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112264.1"
                     /translation="MTIPEEPTQPTPEQPPTWPGWWVILGAVIVLVAILAVVYLGDGE
                     TDDATVTTETTEESGTTSPDVATTEVAETEAPTETTAAS"
     gene            complement(3652..4506)
                     /locus_tag="VFU96_03040"
     CDS             complement(3652..4506)
                     /locus_tag="VFU96_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112265.1"
                     /translation="MNYPLLYLEHLSQRDFELLAQTAGTGGAALKHRMVERPEVIDEL
                     LASRRLFDSIFDSSRELAEPGASSFLAFAALVNRSASDLQDASYVSEWAAPGKRLPVF
                     DVEPLRHFLDDGARRYFLIEFLNSFTSVASGSFVVQTRDGVRRRRFSELDPVALAEMV
                     ELLPPAERPGGYRRLGDVALFLAGVLPDHTARHPYTPAQREWLAQSAVLGPSEALAEG
                     QALHFIEVAGAAWYRRSVDESTGTVSTGPTFLRTVADRFTDARRILNYLADRYLFRHE
                     LGLVGDPG"
     gene            complement(4623..5666)
                     /locus_tag="VFU96_03045"
     CDS             complement(4623..5666)
                     /locus_tag="VFU96_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEU5112266.1"
                     /translation="MGSSESTEGGLSRRDLLKKAGLGGAVFLGGSVLGHTVQHQAQAA
                     ESDYTNPGLVGHSTMHEQGAPDLYGDIHSPPAALGPDALDAHTFPAKPDGLSPGTVRD
                     IEVVVEERLLEVGAGAHFEAWTYNGSVPAPILRATEGDLLRIHVENHTGYPHNIHFHG
                     AHRPEHDGWEPIPPGGETTYEIEAGPAGLHPYHCHAAPLADHIARGLYGMLIVDPPGG
                     RPPATEVALVLSGFSDDRIGRNGIVAWNGVAGFYHHHPIKVPVGEPVRLYLVNMLEHE
                     PVGSFHLHAETFDVYPAGMGERPSSREDTLVLGMGQRAILEFTLPSIGRYMFHPHQHW
                     LAERGAMGWFAAV"
     gene            complement(5653..7302)
                     /locus_tag="VFU96_03050"
     CDS             complement(5653..7302)
                     /locus_tag="VFU96_03050"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEU5112267.1"
                     /translation="MRKGWLLLIAGSALTLAACAGPPEGEPSPAALGDPATESPSEWS
                     GETRLVPQDYPTIQAAVDAATPGDLILIDRGTYREQVEVTTPGLTLRGVDRNEVVIDG
                     EFQRPNGVSVLFADGVVVENMTAINNTSNGFFWNGVRGYRGSYLTAVNNGVYGIYAFD
                     SSDGLFEHSYASGSPDAGFYIGQCDPCQATIADSISEWNGLGYSGTNASSDLYIVNST
                     FRHNVAGIAPNSLDSELLPPFHDVTVVGNLVHDNGNIDAPALAFEWAAQGNGILLAGG
                     GDSYVAKNRVLNHPQSGIAIFPMLDDNFYMSYDHQVVENVVEGSGLADLTLGGPATSG
                     NCFEDNEFSTTLPAALEIKQPCEGLRFPALFEIGSTTAQLGRLFESGLDLQPQVDIAS
                     LPHPGPQPNMPEGPEAPVTPAVDVFAGSRPDIDSIEVPAMPSDLEVTQQKGINVMGVS
                     FASAAGVFFGLYAYVLPLVLYTSWVVLALLDLSRREDLGRGKVVTWMAAILIIPFLGV
                     VAYFIFGRSRIPAWQRWVFMAGGPAVYLLFLGLGMVIGGIV"
     gene            complement(7299..7676)
                     /locus_tag="VFU96_03055"
     CDS             complement(7299..7676)
                     /locus_tag="VFU96_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEU5112268.1"
                     /translation="MKRMVYGLLCVLLVAACGGGDDGESVSDDDVVVQMFDNRYEFTE
                     INIPVGGSVTFVGAGRNPHNAVAADGSWSTEDVAGSLEQLEGEEAMLTFDEAGEYVFF
                     CTFHGNAGGDGMAGTLVVGPPAE"
     gene            complement(7673..8236)
                     /locus_tag="VFU96_03060"
     CDS             complement(7673..8236)
                     /locus_tag="VFU96_03060"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU5112269.1"
                     /translation="MKIEHEFTVGRPPDRVWEFFQDIPSVAQCLPGADLLGQSDDGSY
                     DGKLSVKLGPMAASFEGKATVSPDPESRSAVIDGKGVDRSGGSRGQVKVSYQIAPEGS
                     GSKVGIDADVTLSGPVAQFGRTGLINEMSKRLIGEFVQCLEGKLAAETASEASTIEAG
                     EVKGISLFFASLWSWFTGLFGRGEKQA"
     gene            complement(8292..8750)
                     /locus_tag="VFU96_03065"
     CDS             complement(8292..8750)
                     /locus_tag="VFU96_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEU5112270.1"
                     /translation="MITVSDGVAAGVREDLSGDAIAQILTDAGYEVGRTVVPDEEKAI
                     VSAIHAASSVARLVVTTGGTGLGPRDVTPEATALVLERDAPGLTHLMMSRGLESTPMA
                     ALSRAKAGSIGSSLVVNLPGSTKGSVEGLEAIIDLVPHALDLLAGDTEHR"
     gene            complement(8768..9613)
                     /locus_tag="VFU96_03070"
     CDS             complement(8768..9613)
                     /locus_tag="VFU96_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112271.1"
                     /translation="MTSEVTVSGPLATETIRRVGPPLVVASALGSTLGIVVAQFIDDL
                     GDGILALILGGNAVIYNNRVEVSGVTSDLVWAGGFLLCLILGFLTLFSYPTARGHGVA
                     RLTLLWMVLHLLRQAFFQAVMVPFAADSQLRLAYDALDAPAGLEYVLSAAGAIGLLLM
                     ALSAAAAFLAFAPHHKLISTGSKRVTFAFWIALVPAIASVFASIPFFVPDAGSGVVPA
                     LPLTAVIFLATLAAAPGTTTVYGPEEERETPWPWGLGVTLIVILLFEQLVLRGGVSVN
                     PALWG"
BASE COUNT         1829 a         3216 c         3159 g         1626 t
ORIGIN      
        1 ggtgactcgt ctcctctccg acgtgggcgg gcccatcgtc gccgtgaccg actacatgag
       61 actggtgcct gaccaagtgt cacgatgggt cccggggccg ctcacgactc tcggcaccga
      121 tggattcggc cgctccgatg cccgcaaggc cctgcgccgg ttcttcgaag ttgacgccgg
      181 tcacatcgtg gccgccacgc tctccacctt ggccggccag ggggagatgc ccgaaaccgc
      241 agtgagagat gccttcgatc gctacgggat agaccccgag gccccggacc ccgccatctc
      301 acgctgatga tctcctatcg cgaccttgtc ggccgggctc gggctcagat cgaagagatg
      361 acgcccgaag acctcgagcc gaggttggcc gaggtgctcg tgatcgacgt tcgcgagacc
      421 ggcgagttcg agcaagggtc catcccggga gcccgtctcc tcccccgtgg gttgctggag
      481 cgggacatca caggagtggc ccccgacagg tcacagccgg tcgtggtcta ctgcgccagt
      541 ggcgggcgct cggccctggc cgcggtgagc ttgcaggaaa tgggttacga gacggtgtat
      601 tccctcgccg gtggattcga ccgatggaag tcggagggaa aggcctgggg agccggcgaa
      661 ggcctgaccg ccgagcaacg tgatcggtac tcgagacacg tcatcctcga ggaagtgggt
      721 gaggccggcc aggctcgcct gctcgactcg agcatcctgc tcatcggggc cggagggctc
      781 gggtcgccgg ccgccctgta cctcgcggcc gccggagtgg ggacgatcgg gatagtcgac
      841 tacgacgtgg tggaccggac caacctgcaa cgtcaggtgc tgcacaacct cgaccgcgtg
      901 ggaatgccaa agacggagtc ggcccgggag gcgataacgg cactgaatcc ggacgtcaag
      961 gtggtcacct acaacgagcg gctcgacgcc gagaacgccc tcgacatcat gaagggttac
     1021 gacgtcgtcc tcgatggtgg cgacaacttc cctacccggt acctggtcaa cgacgcctcg
     1081 ctgcacctcc gggtcccggt ggttcatggc tcgatattcc gtttcgaagg ccaggtttcg
     1141 gtgttcgatc cctatgccgg gccctgttat cgatgtctct tccccgagcc accaccaccc
     1201 gagctggccc cgtcctgcgc cgaggcaggc gtcctcggag tgctgccggg aatcatcggg
     1261 tcgatcgagg ccatggaggc catcaagctc ctgctcgacc tcggtgagcc tttgacaggg
     1321 agactcctca tctacgacgc cctggaggag gacttcaact cgctcagaat cgaccgtgac
     1381 cccgactgcc cggcctgtgc cgatcccgat cgtcctccga gaatcgtcga gtacgaccag
     1441 acctgtcggc cgggaggcaa tgtggagcgt gccccagctt cctgaccaga tccgttgggc
     1501 catcctcgcc catgccgaga actgcgtccc gaacgagtgc tgtgggcttc ttgccatcga
     1561 cagggagggg cgtttccgct tcgcctaccc gctgagcaac gccgacccgt caccggtctc
     1621 gtacaccatc gatcccgacg agcattttca ggcgatgcgg cacgccgagt cgatggggtt
     1681 ggagatcgcg ggcgtgttcc actcccatcc aggcggcacg gcgatgccgt cgatgatcga
     1741 tgtgcagagt gccctggagc ccgactggat gtatctgatc gctgccccgg gagagatcag
     1801 aggcttctac attcgcaacg gcgtgatcga agagatcgct ctcgactgag gtcacaccca
     1861 gacggtgacg atcagcgcca cgatgatgta ggcgatgatg gcgttcaggc cattgatcaa
     1921 ccagtatgaa gagcccttct gctcgtagat ctggctcacc agggcactga ccgccccaaa
     1981 gcccacgccc agtgcggcac cgagggcgac cccatcgacg acttccgtcg cgccgattcc
     2041 gcgtgcgatc agcgcgatga caacggtcgt gacgaagtac ccgaccagcg tcgggccgaa
     2101 cagcgatgcc agcggggggc cttctgtggt catgtctacc ttggaggcct tctgccatgc
     2161 cttgctgagt ggtggcaccg aataccagat cgcgctgaac gcgaaccatg cgatagtcgc
     2221 caccacgatt gcaagccagt tcaactcgcc aaagaaatcg aagatcatcg cgtcctcctg
     2281 attggggccg atcggcgatc aaagtaccgg tcgggtcacc gaccgccaag catcggtggt
     2341 cagaggtcga tggcccggtt cacccacacc gacgtgacct ccatgccaac tctctcgtat
     2401 aggccgagcg cgccggtccg ggaatccgtc gagagctccg atcgggtggc cccatgttcc
     2461 cgggcacggg cgaacgcgtc ggccaggagg gcttgcccga gccccatgcc cctctggtcc
     2521 ttgcgaacgg cgatcctctc cacgaagcca acctcgtctg cgagcaggat cagcacggca
     2581 cccaccacgt caccaatccc atcagtcaca acgcggagat tccacggctc gaacccgggt
     2641 ctgtgcaaga cctgagccga gaaatcctcg aagctctgcc ggtcccgcac cgaccattcc
     2701 agaaaggcgt cttcgatgac cgtccagacc gcccgatgat cagccagctc cgcggtcctg
     2761 atcgtgtagc cgggagggag cggcggcgct tcgatgacct ggccttcggg gagggcgaga
     2821 gcccagctcg tccaacggat ccggtagccg aggcgctcga ggagccggtc accggacgag
     2881 cccctgggaa caggcatccc gatcacttgc agaccgcgag ctcgagcctg cccctgcatc
     2941 cagttggcca ggaaggtgcc gattccccgc cctcggtagg tcggatcgac cgcggcgtcg
     3001 cctcgatcgc cccccgagag ctcggcgtag gcgatgagcc ggtcgtcatc gaagatcccg
     3061 atggtgctgg cgcccacgtc gtagctcgga cgttgccagt cgctcacgac atcggcctcc
     3121 tcgatcacga cctcaccgac gtcctccagc tgccctgccg caatcacggc cgtcaccaca
     3181 ctctcgtcac cgggtgccaa cggacgggtg accaggcctt gtgggagagt catgcgcgta
     3241 ttgaagccga agggctggtt ggcaacggtc agttgttcgg caggccggcc atcggatgtc
     3301 taggaggccg ccgtggtctc ggtcggggcc tcggtttccg cgacctcggt tgtcgcgaca
     3361 tcaggacttg tcgtcccgct ctcctcggtg gtttccgtcg tcaccgtggc gtcatccgtc
     3421 tccccgtcgc ccagatagac gactgcgagg atcgcgacca acacgatcac cgcgcccagg
     3481 atcacccacc atccgggcca ggtcggtggc tgctccgggg tcggctgggt tggctcttcg
     3541 ggaatggtca tcgccggtac ctccaatgcg agggcgacac gaagctacga ggcgacagcc
     3601 taggacccaa cggttcaccg gcccgagcaa tgacgtttgg cgaaggccgg cctaacccgg
     3661 gtcgccgacc aggcccagct cgtgacggaa cagatatcgg tcggccaggt agttgaggat
     3721 gcgacgggca tcggtgaaac ggtcggccac cgtgcgtagg aaagtcgggc cggtggagac
     3781 cgtgccggtg gactcatcga cagaccgccg ataccaggcg gcgccggcca cctcgatgaa
     3841 atgcagtgcc tgaccctcgg cgagcgcctc ggagggaccg agcaccgcag attgggcgag
     3901 ccactcccgc tgcgcggggg tataggggtg ccgcgccgtg tggtcgggca agacgccggc
     3961 caggaacagg gcgacatcgc ccagcctccg ataacccccg ggcctctccg cgggtggcaa
     4021 aagctcgacc atctcggcga gggccacagg atcgagctcg ctgaatcgcc gccgtcgtac
     4081 gccatcgcgg gtctggacga cgaagctgcc gctcgccacc gaggtgaacg agttgaggaa
     4141 ctcgatgagg aaataccggc gtgcgccgtc atcgagaaaa tgtcgcagtg gctcgacatc
     4201 gaagaccggg agccgtttcc cgggcgctgc ccattccgag acgtagctcg cgtcctggag
     4261 atcgcttgca gacctgttga caagagcggc aaaggcgagg aaggaagacg cccctggttc
     4321 ggccagttcg cgagagctgt cgaagatcga gtcgaagagt cgccgggatg ccaggagctc
     4381 gtcgatcacc tcggggcgtt ccaccattcg gtgcttcagc gccgcgccgc cggtcccggc
     4441 ggtctgtgcc agcagctcga agtcccgttg gctgagatgc tccagataga gcagtggata
     4501 gttcatatcg cacccggccc atcttccggt ccgggcctga tggcccgaac caacaggttc
     4561 atgtcacctt atgaatcggc accgtcaacg ggtgggatca cgggtcgatc gacccaatcc
     4621 gcctagaccg cggcaaacca gcccatggcc cctcgttcgg cgagccaatg ctggtgcggg
     4681 tggaacatgt agcgcccaat gctgggcagc gtgaactcga ggatcgcccg ctgacccatg
     4741 ccaaggacga gggtgtcctc gcgggacgat ggtcgttctc ccattcctgc cggatacacg
     4801 tcgaacgtct ccgcatgaag gtggaacgag ccgaccggct cgtgctccag catgttgacc
     4861 agatagagac ggacaggctc accgaccggg accttgatcg gatggtggtg gtagaagccg
     4921 gccacgccat tccaggcaac gatgccattg cggccgatcc ggtcgtcgga gaaccccgag
     4981 agaacgagag cgacctcggt ggccgggggc cggcctccgg gcgggtccac gatcaacatg
     5041 ccatagagac ctcgcgctat gtgatcggcg agtggggcgg cgtgacagtg gtagggatgg
     5101 agcccggccg ggccggcctc gatctcgtac gtcgtctcgc cgccgggggg aatcggctcc
     5161 catccgtcgt gctcgggccg atgcgcccca tggaaatgga tgttgtgcgg gtagcccgtg
     5221 tggttctcga catggattcg cagcagatcg ccttcggtgg cccgcaggat cggtgccggg
     5281 accgacccgt tgtaagtcca ggcctcgaaa tgtgcaccgg cgcccacctc gaggagacgc
     5341 tcctcgacga cgacctcgat gtcccggacg gtccccggag acaggccgtc aggcttcgcg
     5401 ggaaaggtgt gcgcatcgag tgcatcgggc cccaaggccg caggcgggga gtggatgtcg
     5461 ccatagagat cgggcgcgcc ttgctcgtgc atggtggaat ggccgaccag cccggggttg
     5521 gtgtagtcgc tctcggcggc ctgagcctga tgctggacgg tgtggccgag gaccgaaccc
     5581 ccgaggaaga ctgcgccacc gagacccgcc ttcttcagga ggtcacgtcg ggacaggccc
     5641 ccttccgtcg actcagacga tcccaccgat gaccattccg agacccaaga acagcagata
     5701 caccgccggt cccccggcca tgaacaccca ccgctgccac gcagggatcc tgctcctgcc
     5761 gaagatgaaa taggccacca caccgaggaa ggggatgatc aggatcgccg ccatccatgt
     5821 aacgaccttg ccgcggccca ggtcttcccg ccgcgacaag tccaataggg ccaggaccac
     5881 ccatgaggtg tagaggacca gggggaggac gtaggcatag agcccgaaga agacccctgc
     5941 ggccgaggca aagctcacac ccatgacgtt gattcctttc tgctgggtga cttccaagtc
     6001 cgagggcatc gccgggacct cgatgctgtc gatgtctggc cgcgatccgg cgaacacgtc
     6061 gactgccggt gtgacggggg cctcgggccc ttccggcatg ttcggctgcg gccccgggtg
     6121 aggcagggag gcgatgtcga cctgaggctg gagatcgagc ccactctcga agagacggcc
     6181 gagctgtgcc gtcgtcgacc cgatctcgaa caacgcagga aaccgaaggc cctcgcacgg
     6241 ctgcttgatc tccagggcgg cgggcagagt cgtggagaac tcgttgtcct cgaagcagtt
     6301 gccggatgtc gccggccctc ccaaggtcag gtcggcgaga cccgatccct cgacgacgtt
     6361 ctccacgacc tgatggtcgt aggacatgta gaagttgtcg tcgagcatgg ggaagatggc
     6421 gatcccgctc tgtgggtgat tgagaactcg gttcttggcc acatacgagt cgccaccccc
     6481 ggcgagaagg atgccgttgc cttgcgccgc ccactcgaaa gcgagagcgg gagcgtcgat
     6541 gttcccgttg tcgtgcacga ggttgcccac gacggtcaca tcgtgaaagg gcgggagcaa
     6601 ttcgctatcc aggctgttgg gagcgatccc ggcgacgttg tggcggaatg tcgagttgac
     6661 gatgtagagg tcggacgagg cgttggttcc cgagtacccc agcccattcc actccgagat
     6721 cgagtcggcg atggtcgcct ggcaggggtc gcactgcccg atgtagaagc cggcgtccgg
     6781 cgacccggag gcgtacgagt gctcgaagag gccgtcgctc gagtcgaatg cgtagatccc
     6841 atagaccccg ttgttgaccg cagtgagata cgacccgcga tatccgcgga ccccgttcca
     6901 gaagaacccg tttgacgtgt tgttgatcgc agtcatgttc tcgaccacca ccccatcggc
     6961 aaagaggacg ctgaccccat tggggcgctg aaactcgccg tcgatcacta cctcgttgcg
     7021 atccacgccg cgcagagtca ggcccggggt ggtcacctcg acctgctcgc gataggtccc
     7081 gcgatcgatg aggatcaggt ccccgggcgt ggcggcgtcc accgccgcct ggatcgtcgg
     7141 gtagtcctgg gggacgagtc tcgtctctcc cgaccattcg gacggcgatt cggtcgccgg
     7201 gtctcccagg gcggccggcg acggctcccc ctctggtggg ccggcacatg ccgcgagtgt
     7261 cagggcgctt cccgctatca gcaggagcca gcccttcctc attcggcggg agggccaacg
     7321 acgagggtgc cggccattcc gtcccctccc gcgttgccgt ggaacgtgca gaagaagacg
     7381 tactcacccg cctcgtcgaa ggtgagcatt gcctcctcac cttcgagttg ctcgagactg
     7441 cccgccacat cctcggtcga ccaggagccg tcggccgcga cggcgttgtg cgggtttcga
     7501 cccgccccga cgaacgtcac cgacccaccg acgggaatgt tgatctcggt gaactcgtag
     7561 cggttgtcga acatctgaac gacgacgtca tcgtctgaca ccgactcgcc atcatcgcca
     7621 cctccgcagg ccgcgaccag cagcacgcag agcaagccgt aaaccatccg cttcatgcct
     7681 gtttctcccc tcgcccgaac aaacctgtga accaactcca gagtgaggcg aagaagagcg
     7741 atatcccttt cacctcaccc gcctcgatcg tcgacgcttc cgacgctgtc tcggcagcca
     7801 gcttgccttc gagacactgg acgaactcgc cgatgaggcg cttcgacatc tcgttgatga
     7861 gcccggtgcg gccgaattgg gcgactggac cagagagggt gacatcggcg tcgatcccga
     7921 ccttcgaccc gctgccctcg ggggcgattt gataagagac tttgacctgg cctctcgatc
     7981 caccgctccg atcgaccccc ttcccatcga tcacggcgct cctcgactcc ggatccgggc
     8041 tcaccgtcgc cttcccctcg aacgaggccg ccatgggccc gagcttcacc gagagcttcc
     8101 cgtcgtagga gccatcatcg gactggccga gcaagtcggc accggggagg cactgcgcca
     8161 cggacgggat gtcctggaag aattcccaca cgcggtcggg cgggcgaccc acggtgaatt
     8221 catgttcgat cttcatggtc ctcctgcggc gtgaagcggg gaagacgcta gtgaacggcg
     8281 gcgccgtatc cctaacgatg ctcggtgtcg ccggcgagga ggtcgagagc gtgcggcacg
     8341 aggtcgatga tggcctccag gccttcaacc gaccccttgg tggacccggg cagattgacc
     8401 acgagcgaag atccgatcga gccggccttg gccctcgata gtgccgccat cggcgtcgat
     8461 tcgaggcccc tcgacatcat caaatgggtc agtccgggcg catctcgctc caagaccagc
     8521 gccgtcgcct cgggggtgac atcgcgcggt cccaggccag tgccacccgt cgtgaccacc
     8581 aggcgtgcca ccgacgaagc cgcgtggatc gccgagacaa tggccttctc ctcgtcaggg
     8641 accactgtgc gtcccacctc gtagccggcg tcggtcaaga tctgagcgat tgcgtcgcca
     8701 gacagatcct cccggacccc ggcggcgaca ccgtcactca ccgtgatcac gtgagcgatt
     8761 ttccgagtca accccacaga gccgggttga ccgaaactcc gccgcggagc accaattgct
     8821 cgaagaggag gatcacgatc agggtcactc ccagacccca tggccatgga gtctcccgct
     8881 cttcctcagg cccatagacg gtggtggtcc cgggcgccgc cgcgagcgtc gccaggaaga
     8941 tgaccgcggt caggggcagg gccggcacta cgccgctccc cgcatcgggg acgaagaacg
     9001 ggatcgaagc gaacaccgag gcgatagcgg gcacgagggc gatccagaag gcgaacgtga
     9061 cgcgcttgct cccggtggag atcagcttgt ggtggggagc gaaagccagg aatgcggccg
     9121 ccgcggatag tgccatcaac aggaggccga tcgcgcctgc ggccgacaac acgtattcga
     9181 gcccggccgg cgcatcgagg gcgtcgtagg cgagtctgag ctggctatcg gcagcgaaag
     9241 ggaccattac ggcctggaag aaggcctgac ggaggaggtg gaggaccatc cacagcagag
     9301 tgagacgggc gaccccatgg ccacgcgccg tgggatacga gaacagggtc aggaagccca
     9361 ggatcaggca gaggagaaaa ccgcccgccc agaccaggtc tgaggtgacg ccgctgactt
     9421 cgacccggtt gttgtatatg acggcgttcc cgccgaggat caaggcgagg atgccgtctc
     9481 cgaggtcatc gatgaattga gcgaccacga tccccagcgt cgaccccaga gccgaggcca
     9541 ccacgagggg agggccgact ctcctgatcg tctcggtcgc cagggggccc gatacggtta
     9601 cttcactagt cacaaacggt catcctaaga cgagggtttt cttcaccacg acataggtga
     9661 tcggcacgtg aatgcggctt ctaaatggag acaatcagag tccccgcagc ccagatcacg
     9721 agcagtccga tcgagacgag aaggacaggc gcggtccgtc gccaattcat ggctgaccac
     9781 agcgcggtca ccgccaaacc tgccgccatg accccgctcc accacacggg
//
LOCUS       DASTXO010000144         2531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1777263, whole genome shotgun sequence.
ACCESSION   DASTXO010000144 DASTXO010000000
VERSION     DASTXO010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1777263"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFU96_03075"
     CDS             complement(<1..306)
                     /locus_tag="VFU96_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5112272.1"
                     /translation="MQRNSVQVQLLLAIAFLSVGFGVLYPDQFATFRNFENITRQGAT
                     LVVVAVGQTFALIVGGFDVSVGSTMGLSSVVGALVMIEYGTTAGVLAGLGAGLAVGLV
                     "
     gene            complement(494..967)
                     /locus_tag="VFU96_03080"
     CDS             complement(494..967)
                     /locus_tag="VFU96_03080"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HEU5112273.1"
                     /translation="MSTRRREIVDEIDRGIIGILQKNGRTPNREIARVLDISEATVRN
                     RVSRLLTEDLINIVAAPTPKAVGLTMSAIIGVSVEMRRLDQVVDTIVDYGEVRYLGIS
                     TGQHDIIIEAFFEDQEHMLQFVTKKLGHLEGVLNLETSVILKVAKFSYEWEIPQL"
     gene            1084..>2531
                     /locus_tag="VFU96_03085"
     CDS             1084..>2531
                     /locus_tag="VFU96_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112274.1"
                     /translation="MAETEVPTTGAGVFSRPASGLVRVAGTMDVFIFNVGLVSIGIAI
                     AFNQLYGPSLYPGAAPWLSTLLAAFGMLFVAATFYSWSVTFPRSGGVYVSLSRSVGPG
                     LGFVLSLIETIILMYYAALAASLIVTVGLSSFLATVGFIGENATLAEWAATTASPAGI
                     FWIGTGVLVLAGLLLTSGTRRYFTVQKVLFVIAVIGTIVIALVLLFGDTDTFFANFER
                     LTGLTEGEVVTAAEGFGWTSAPIGFAASFAFLVWPLLPLLGAVQSVGIGGEIKSVRRT
                     QLFGILGALIITALVIALFDILATKVFTYDFQGAIGFNSITGLFDPASDAWAFSTEST
                     IGASPWFTVLVGILADNIWLAVIVMATFFAWIWFWIPAEIAYTTRTMIAWSFDRIAPK
                     RLGEVSSRFGTPTLAIWISTAGAIVFMWFIAYQAIALLTLVEALAVVWGTAMISAVVF
                     PRTRRQLFEVSPASEQRLGGIPIMSITGALAA"
BASE COUNT          420 a          817 c          760 g          534 t
ORIGIN      
        1 gaccaacccg actgccagcc cggcgccgag accggcaagc accccagcgg tggtgccgta
       61 ctcgatcatg accagggccc cgaccacgct ggaaagtccc atggtcgagc ccaccgagac
      121 atcgaaccct cccacaatca gcgcgaacgt ctgtcccaca gccacaacca cgagggtggc
      181 tccttgccgg gtgatgttct cgaagttcct gaaggtcgcg aactgatcgg ggtacaggac
      241 gccaaagcca accgagagaa atgcgatcgc tagaagcagc tggacctgga cagaattccg
      301 ttgcaagaaa cctgaggacg gtctcctcgt tggccgcgat gaagtttcgc caagtgtccc
      361 gggcgcgtcc aagggctcgc cgccttctct cagttctgtt gtcccagcca ccgacgcagc
      421 cgcatcatgg agttcggcga ctgtatcaat cgattgcagc cctcgcccaa atgttgccgc
      481 tgatgcctat ccactacagc tggggtattt cccattcgta ggagaacttg gcgaccttga
      541 ggatgaccga ggtctccaga ttgaggacgc cttcgaggtg gcccagcttc ttggtgacga
      601 actgaagcat gtgctcctgg tcctcgaaga aggcctcgat gatgatgtcg tgctggccgg
      661 tcgaaatgcc gaggtaccgc acctccccgt agtcgacgat ggtgtcgacc acctggtcga
      721 gccgtcgcat ctccacagac actccgatga tggccgacat ggtgagcccg accgctttcg
      781 gggtgggggc ggcgacgatg ttgatcaggt cttcggtgag cagccgggag acccggtttc
      841 gcacagtcgc ctcactgatg tcgaggaccc gggcgatctc ccggttcggg gtccgtccat
      901 tcttctggag gatcccgatg atgccccgat cgatttcatc gacgatctcg cgtcgccttg
      961 tgctcattgc gcaggaatga tgccaggaag cgctcccttg cgcagagaaa tcctccagtc
     1021 tcgccagcat gcgcacctct agccgatcta ggctgcgcgt cacagcagag atggaggttg
     1081 gcgatggctg agactgaggt cccgaccaca ggagccggcg tcttttcaag gccggcatcc
     1141 ggtttggtcc gggtggcggg gacgatggac gtcttcatct tcaacgtggg gctggtgtcg
     1201 atcgggatcg cgattgcctt caatcagctc tacggcccga gtctctatcc cggtgccgcc
     1261 ccttggctct ccacgctgct cgctgccttc gggatgttgt tcgtggcagc cacgttctat
     1321 tcgtggtcgg tcacgttccc cagatccggt ggcgtctacg tctcgctctc ccgatcggtc
     1381 gggccgggtc tgggcttcgt cctcagcctg atcgagacga tcattctgat gtactacgcc
     1441 gcattggccg catccctgat cgtcacggtc ggcctgtcgt cgtttctggc aacggtcgga
     1501 ttcatcgggg agaacgcgac cttggccgag tgggctgcca ctactgcctc gcccgcagga
     1561 atcttctgga tcggcaccgg cgtgctggtc ctggccgggc tgctcctgac ctccggtacc
     1621 cgccgctact tcaccgtgca gaaggtcctt ttcgtcatcg ccgtgatcgg cacgatcgtc
     1681 atcgccctgg tccttctctt cggtgacacc gacaccttct tcgccaactt cgagaggctc
     1741 accgggctca ccgaaggcga ggtggtcacg gcggcggaag ggttcgggtg gacatcggca
     1801 ccgatcggat tcgcggcatc attcgccttc ctggtctggc cgctgctgcc gttgctgggt
     1861 gcggtccaat ccgtgggcat cggcggtgag atcaaatcgg tgcgccgaac acagttgttc
     1921 ggaatcctcg gtgccttgat catcaccgcc ctcgtgatcg ccctcttcga catcctggcc
     1981 accaaggtgt tcacctatga cttccagggg gccatcggct tcaactcgat caccggcctc
     2041 ttcgatccgg cctccgacgc ctgggccttc tcgaccgagt cgaccatcgg cgccagccca
     2101 tggttcacgg tgctggtggg catcctggcc gacaacatct ggctggcagt gatcgtgatg
     2161 gcgaccttct tcgcctggat ctggttctgg atcccggccg aaatcgccta caccacacgg
     2221 accatgatcg cctggtcgtt cgaccggatt gccccgaagc gactcgggga ggtgagcagc
     2281 cgattcggaa caccaaccct ggccatctgg atctccactg ccggggcgat cgtgttcatg
     2341 tggttcatcg cctaccaggc gatcgcactc ctcacactgg tcgaggcgct cgccgtggtc
     2401 tggggaaccg ccatgatctc ggcggtggtc ttcccacgga cccgtcgtca gctgttcgag
     2461 gtgtctccgg cctccgagca gaggctcggc ggcatcccga tcatgtcgat caccggggca
     2521 ttggccgcga t
//
LOCUS       DASTXO010000145         5510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_709103, whole genome shotgun sequence.
ACCESSION   DASTXO010000145 DASTXO010000000
VERSION     DASTXO010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5510
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709103"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VFU96_03090"
     CDS             <1..379
                     /locus_tag="VFU96_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112275.1"
                     /translation="EPDMHLVTMVIVCVAAIGIALGAGTPSASAEGCASTCSVGAVGT
                     GGESSDGNAEGFHVQQPSTGFPGSNFSNSGNQIAGNISVTGASQGSASGALTPQGAVV
                     GHFTGVSAIFFGMDGDCNGVCTP"
     gene            750..2555
                     /locus_tag="VFU96_03095"
     CDS             750..2555
                     /locus_tag="VFU96_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5112276.1"
                     /translation="MRSKTARTIGATAVLVGVVIAAAIQMRPEEEAPAEIAAPVGTTT
                     LRVPVSSLAGADAPFEYRVGLLAGVSTANYWEYVGEQPTAWNAYVLGPTKPALYAVDP
                     ASNALEPEVAASAPVLPTWDREGWRVRLDLGSDLSWSDGRPVTAADVVYTFETVRRLG
                     LEGGWADAYPEEVEGIIAESPTRLRIEFSRRPGLGLWPHGVGLAPIMPSHVWAPQTDG
                     IGTAAALYDLDPDVDVSGGPLQIVEVTDSRIATVANPGYPEDGVPNVVYSIFADEATA
                     IAAMKQGTIDTVLDPNGLSDEGAATLAGTPGVAIEQSPANSVRYLGFNLTRDPMSNPE
                     FRQSLALLLDRGWATEKVVPNASAAYTMLSSANASWFDDGEAAAISHPYARTLQTRVS
                     AAISGLESVGYSWETSPTVVGGTLTPGTGLTIDGQVPAPLTILTPGDEYDPARPDYTT
                     RIEATLETLGFDVRPVVTDFDTVVDLAFTEDHSGRRQYDMYVLGWTLGNPALPDYHRW
                     LFAGDGPANSTGYANPDFEASLARFDRATAAKDAKDALWGMEQVLARDLPYLVLYHPN
                     LIEGYRADRVRFEEHDVLGGIQGRLGGLTDLSPVS"
     gene            2580..3176
                     /locus_tag="VFU96_03100"
     CDS             2580..3176
                     /locus_tag="VFU96_03100"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEU5112277.1"
                     /translation="MTGVEASNRILTIPNLVSFVRLLGIPVFWWVLLVEDNVALAAWL
                     IFIIGWTDWIDGYLARRLGQVSELGKILDPVADRLMIASAVIGGLIAGVLPTWFGWGL
                     IIREILVGAVALNLAVRGGGTVEVRFLGKTATFALYGAIPAFYLAAAGAAEWLLWPVA
                     WILGLIGLVLYWYVAFLYVGDSRAKLAALKSSPDPEEA"
     gene            3176..3553
                     /gene="gcvH"
                     /locus_tag="VFU96_03105"
     CDS             3176..3553
                     /gene="gcvH"
                     /locus_tag="VFU96_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020524343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEU5112278.1"
                     /translation="MEIPDDLLYTEEHEWIRQVGDSLVRIGITDYAQDQLGDVVFVEL
                     PVIGKVVAKDDVLVEVESTKSVGEVYAPFPGTITDVNESISESPELVNQSPYEDGWLV
                     LLSVDGEMPTGLLDAARYRDLTE"
     gene            3600..4043
                     /locus_tag="VFU96_03110"
     CDS             3600..4043
                     /locus_tag="VFU96_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEU5112279.1"
                     /translation="MTDEREPGDEQPEPRLEVDGDPTVVYVPIQDRADHPMTREEASS
                     LDGVSGFALIVERGPKTGMTYLLPGGNTTVGRHPESDIFLNDVTVSRQHCRFLVDDDG
                     LVVEDSGSTNGTYVNDARVDQATLKAGDEVLVGRFHFVVAHGDER"
     gene            4033..4710
                     /locus_tag="VFU96_03115"
     CDS             4033..4710
                     /locus_tag="VFU96_03115"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU5112280.1"
                     /translation="MSDSQSLSIGEVINLLRDDFPDVSVSKIRFLESQGLIDPGRSDS
                     GYRQFDQEDLARLRFILQQQRDHFLPLKVIKSKLTLWERGEDLASKTAVPAPSLESKG
                     EPLSRDDLLRRSGLSPAELDDLLQVGVVRSIGDPEVFAPETGIVATEAKRLMDQGLEA
                     RHLRTVRLSVDREADLLRQLAAPLLRANNPEARARAMDLLELSGGSVQAMHRAMLEVE
                     LKQQITS"
     gene            4707..>5510
                     /locus_tag="VFU96_03120"
     CDS             4707..>5510
                     /locus_tag="VFU96_03120"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEU5112281.1"
                     /translation="MKRSIRAVGALTLVLFVLAASASAAPFTYPAPVLETFPAPEAPD
                     VTAASWILYDESTDTVVAEFNADEERAMASVTKIMTGLLTVENSSPDDMVTISERAAN
                     TGEKEIELVAGETVSMDALFKALMIHSANDAATAIAEHISGSVEGFVTLMNQRAAELG
                     LTHTSFANPHGLDAPDHYTSARDLLHLARVAMENPQFEEVVKAKKLVFPPAPDGTPRT
                     GSSTNLMLWSYQGSLGVKTGFTAQALLTYVAAAEREGRRLYAVLLGSDGE"
BASE COUNT          987 a         1781 c         1759 g          983 t
ORIGIN      
        1 agaacctgac atgcacctgg taaccatggt catcgtatgc gtcgctgcaa ttggcatcgc
       61 gctgggagcc ggcacaccgt cggcttcggc ggagggatgc gcctccacct gttccgtcgg
      121 cgctgtcgga acaggcggcg agagctcgga cggcaacgcc gaaggtttcc acgtccagca
      181 gccgagcacc ggcttccctg gctcgaactt ttccaactcc ggcaaccaga tcgccggcaa
      241 tatctcggtc acgggcgctt cacaaggcag tgcatccggt gcgctcacgc cgcagggtgc
      301 cgtggtcgga cacttcaccg gcgtctctgc catcttcttc ggcatggacg gcgactgcaa
      361 cggagtatgt accccctaag ccccgatcac acgagaggtc gagtgaagtg aagcctgtcg
      421 ccgcttgagg gcggattgct gctcggggcg atcctcggtg ctcacatcgt gcggagtaat
      481 tcccgtcgtc gtcggtgaac gagcccccag gatcggaagc atccgcaagc gccacacggg
      541 cccacagccc tgacccagca cgaccagagc cagagtcagg atcgaggcaa cccgcgtcga
      601 ctcaaggcgt ctcaacatcg agtctcccgg ttcgagtctc agaatggtcg ccaccaggcc
      661 atatagaccg gcgaacctat cgcgggggag catcctcctg ctggaacgat ggtcaaggac
      721 cacctttcac ctgccgattc gataattgca tgcgcagcaa gacggcaagg acgattggtg
      781 cgacggcggt gttggtcggc gtcgtaatcg ccgcggcaat tcaaatgcga cccgaggaag
      841 aggcgccggc cgagatcgcc gcccctgtgg gcaccaccac cctccgggta cccgtgtcga
      901 gcctggcggg agcggacgcg ccattcgagt accgggtcgg tctcctcgca ggggtgagca
      961 cagccaacta ctgggagtat gtcggggagc agcccaccgc ttggaacgcc tacgtgctcg
     1021 gcccgaccaa gccggcgctg tatgcagtgg acccggcatc caatgccctc gaaccagagg
     1081 tggccgcgtc ggccccggtg ctccccacat gggaccgaga gggctggcga gtgcggctcg
     1141 atctcggcag cgacctctcc tggtccgatg gtcgcccggt gacggccgcc gacgtggtct
     1201 atacgttcga gacagtgcgg agactcgggc tcgagggggg ttgggccgat gcctatcccg
     1261 aagaggtcga ggggatcatc gccgaatctc cgacccgatt gaggatcgag ttctcgcgtc
     1321 gcccagggct cggtctgtgg ccacacgggg tcggtcttgc gcccatcatg ccgtctcatg
     1381 tgtgggcacc acagaccgac gggattggca ccgcggctgc gctctatgac ctcgatccgg
     1441 acgtcgacgt ctccggtggc ccgcttcaga tcgtggaggt caccgacagc cggatcgcca
     1501 ccgtggccaa ccccggctac ccggaggacg gcgtcccgaa tgtcgtctat tccatcttcg
     1561 ctgacgaagc caccgcgatc gcggccatga agcagggcac gatcgacacc gtgctcgacc
     1621 cgaacggtct ctcggacgag ggagccgcca ccctggccgg gacacccggt gtcgccatcg
     1681 agcagagccc ggccaactcg gtgcgatatc tcggcttcaa cttgacccgg gacccgatgt
     1741 ccaatcccga gtttcgccag agcctcgccc tattgctcga ccgagggtgg gccaccgaga
     1801 aggtcgtgcc aaatgcatct gctgcctaca ccatgctgtc ctccgccaat gcctcatggt
     1861 tcgacgatgg cgaggcggca gcgatctccc acccctacgc caggaccctg cagacccggg
     1921 tctcagccgc catctctggc ctggaaagtg tcggctactc gtgggagacc tcgccgaccg
     1981 tggtcggcgg caccctgacg cctgggacag gcttgaccat cgatggccag gtcccagcgc
     2041 ccctgaccat cctgaccccg ggggacgagt acgacccggc gcggcccgat tacacgacgc
     2101 gaatcgaggc gacgctcgag actctcggat tcgacgtgag acccgttgtc acggatttcg
     2161 acacggtcgt cgatctcgcc ttcaccgagg accattcagg ccggcgccag tacgacatgt
     2221 atgtcctggg gtggacgctg ggcaatccgg cgctccctga ctaccaccgc tggctgttcg
     2281 ccggggacgg ccccgccaac tcgaccggat atgcgaaccc ggatttcgaa gccagcctgg
     2341 cccgattcga tcgggcgacg gcggccaagg acgccaagga cgcgttgtgg ggcatggaac
     2401 aagtgctggc acgtgatctg ccctatctcg tgctgtatca cccgaacctc atcgagggct
     2461 accgggccga tcgggtgcga ttcgaggagc acgatgtgct cggcgggatc cagggtcgtc
     2521 tcggcgggct caccgacctg agccctgtgt cctgaccggc ggcagttagc ctccgcgccg
     2581 tgaccggcgt ggaagcaagc aaccgcatcc tcacgattcc caacctcgtc tcgtttgttc
     2641 gcctgctggg catccctgtc ttctggtggg tgcttctcgt cgaggacaac gtggcactcg
     2701 cggcatggct catcttcatc atcggctgga ccgattggat cgacggctac ctggcccggc
     2761 gcctggggca ggtgagtgag ctcggcaaga tcctcgatcc ggtcgctgat cgtctgatga
     2821 tcgcctcggc tgtgatcggc ggcctcatcg ccggggtgct gccgacctgg ttcggctggg
     2881 ggttgataat ccgggagatc ctcgtggggg cagtagccct caatctcgct gttcgtggtg
     2941 gaggcacggt ggaggtgcgc ttcctgggta agacggcgac gtttgccctc tatggggcga
     3001 taccggcctt ctatctcgcc gcagccgggg cggccgagtg gctgttgtgg ccggtggcat
     3061 ggatccttgg cctcatcggc ctggtgctct actggtacgt cgccttcctc tatgtcgggg
     3121 acagtcgagc gaagctcgca gcgttaaagt cgtcgcctga ccccgaggag gcttgatgga
     3181 gattcctgac gatctgctct acaccgagga acacgagtgg atccggcagg tcggcgattc
     3241 tttggtgcgg atcgggatca ccgactacgc ccaggaccaa ctaggcgatg tggtcttcgt
     3301 cgagctgcct gtcatcggga aagtcgtcgc gaaggacgac gtcctggtcg aggtggagag
     3361 cacaaagtcg gtgggggagg tctacgcgcc gtttcccggc acgatcaccg atgtgaacga
     3421 gtcgatctcg gagagcccag agctggtcaa ccagagcccg tacgaggacg gctggttggt
     3481 gttgttgagt gtggacggcg agatgcccac cggtcttctc gacgcggccc gataccggga
     3541 cctcaccgag tagacccggt caccgggctc acagccgtca actccctatc atctccgtcg
     3601 tgaccgatga acgcgagccc ggggacgagc agcccgaacc tcgactagag gttgacggtg
     3661 atcccaccgt ggtctacgtc ccgatacagg accgggcgga ccatccgatg accagggaag
     3721 aggcctccag cctcgatggt gtgtctggtt tcgccctgat cgtcgagcga ggccccaaga
     3781 ccggaatgac ctacctgctc ccgggaggga acaccaccgt gggccggcat cccgagagtg
     3841 acatattcct caacgacgtg accgtgtcac gtcagcactg ccggttcctc gtcgacgacg
     3901 atggactcgt cgtggaggac agcgggtcga ccaacggcac ctatgtgaat gacgctcggg
     3961 tcgaccaggc caccctgaag gcgggtgatg aggtcctggt ggggagattt catttcgtcg
     4021 tcgcccatgg cgatgagcga tagccaatcg ctgagcatcg gagaggtgat caacctgctt
     4081 cgtgacgact tcccggacgt ctcggtctcg aagatccgtt tcctcgaatc ccaagggttg
     4141 atcgatcccg gtcggtcaga cagtggatac cgccagttcg atcaggagga cctggcgagg
     4201 ctccggttca tcctgcaaca gcagcgagat cacttcctcc ctctcaaagt catcaagtcc
     4261 aagctcaccc tgtgggagcg tggcgaagac ctcgcgtcga agaccgccgt gccggcgccg
     4321 agcctggagt cgaagggcga gccactcagt cgggacgatt tgctccggcg atcgggcctc
     4381 tccccggccg agctggacga cctgctccag gtgggagtgg tgcgatcgat cggagacccc
     4441 gaggtattcg ccccggaaac aggtatcgtc gccaccgagg cgaagcggct aatggaccag
     4501 ggcctcgagg cccgtcacct ccgcaccgtg cgtctctcgg tcgacaggga ggcggacctg
     4561 cttcgtcagc tcgccgcccc gctgcttcgg gccaacaacc ccgaggcgag ggcgcgggcc
     4621 atggacctcc tcgagctgag tgggggatcg gtccaggcga tgcatcgcgc catgctcgag
     4681 gtcgagctaa agcaacaaat cacttcatga aaagatccat tcgggcagtt ggggcgctaa
     4741 ccctcgtctt gttcgtcttg gccgcgtccg cttcggcagc cccgttcact tatcccgcac
     4801 cggtcctgga gacatttccg gcgcctgaag cgcctgacgt gaccgcggcg tcgtggatcc
     4861 tctatgacga atcaactgac acggtcgtcg ccgagttcaa cgccgacgag gagcgggcga
     4921 tggcctcagt gacgaagatc atgacggggc tgctcacggt cgagaactcc tccccggacg
     4981 acatggtgac gatctcagag cgcgccgcca acaccggtga gaaggaaatc gagttggtag
     5041 ccggggaaac cgtctcgatg gacgcgttgt tcaaggcgct gatgatccat tcggccaacg
     5101 acgcggccac cgccatcgcc gagcacattt cgggctcggt cgagggcttc gtcaccctca
     5161 tgaaccaacg agccgcagag ctgggtctca cccacacatc gttcgcaaac cctcacggac
     5221 tcgatgcccc cgaccactac acgagtgccc gcgacttgct ccatctcgcg agggtggcga
     5281 tggagaaccc ccagttcgaa gaggtggtga aggccaagaa gttggtcttc cctccggccc
     5341 ccgatggcac acctcggacg ggaagctcca cgaatctcat gctctggtcg taccagggct
     5401 cactcggtgt caagaccggg ttcaccgccc aagccctact cacctatgtg gcggcggcag
     5461 agcgggaggg ccgccgcctc tatgcggtcc tgctcggctc cgacggggag
//
LOCUS       DASTXO010000146         2814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1740095, whole genome shotgun sequence.
ACCESSION   DASTXO010000146 DASTXO010000000
VERSION     DASTXO010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1740095"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..155
                     /locus_tag="VFU96_03125"
     CDS             <1..155
                     /locus_tag="VFU96_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018873031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEU5112282.1"
                     /translation="RAEIRANLARALGIIPDLVSVKATTTDGLGFIGRGEGLAAVAVV
                     TVTPLS"
     gene            165..1556
                     /gene="cysS"
                     /locus_tag="VFU96_03130"
     CDS             165..1556
                     /gene="cysS"
                     /locus_tag="VFU96_03130"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041717996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU5112283.1"
                     /translation="MLRLYSTLHRAVVPFEARDEGEVAMYLCGPTVQSEPHVGHGRSA
                     VAFDVIRRYLMWSGHRVTYVRNVTDIEDKIIAAAAETGESVEVLAERMAARFASGYRA
                     LGVLPPDLEPKATEHIPEIIDLIRVLIDRGLAYASEGDVYFSVRADESYGKLSGRNPD
                     ELRSGVRIEVGEAKHDPLDFALWKAAKPGEPWWDSPWGPGRPGWHIECSAMATRYLGN
                     DFDIHGGGTDLIFPHHENEIAQSEGASGLTFARYWLHNGMVNLGGEKMAKSTGILVDL
                     ASITEKHGGRALRLLLLRAHYRSPIEYSDELLTESEDALDRLQRFRDRASSGDPDPEA
                     IDRFREVMEDDFGTPLAVSLLFDLVRDGNRLLDEGGDAAAIAGAVESIVGVLGLDDAS
                     VPAEESLDLGDLPGRFGLEGNGHDVVDRLVELRAAAREERRFEDADAIRDGLEKAGVT
                     LEDGPDGTRWLRK"
     gene            1531..2289
                     /locus_tag="VFU96_03135"
     CDS             1531..2289
                     /locus_tag="VFU96_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU5112284.1"
                     /translation="MAPAGYGNRVEGIHSVAAAASRGRVRKLWVEEKRLGRAEIAEIV
                     EAVGRERTALLRDVRHMAETEAPQGVVAECDPIMPAPVDDLAGPGAALVVLDHIEDPH
                     NVGAVARSALSAGMTGMVVSSRRAAPLSAAAFKAAAGALETLSVSIVGSIPEALSRLK
                     DRGVWIVGLEASSARSLFGLDLLTESVAVVVGGEGAGLSVLSAKRCDVLASIPMASNF
                     ESLNASVSAALACFEIMRVRAGADPSLQSDLPPG"
     gene            2280..2355
                     /locus_tag="VFU96_03140"
     tRNA            2280..2355
                     /locus_tag="VFU96_03140"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2313..2315,aa:Thr,seq:tgt)
     gene            2570..>2814
                     /locus_tag="VFU96_03145"
     CDS             2570..>2814
                     /locus_tag="VFU96_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112285.1"
                     /translation="MEQSLDRPMEAWSDDEVLEQYRSMTLNMSNSEGMSRLDLSAITE
                     EMLRRGLSFPDVSAATRVTDTVDWSGEGGGEDPGSGAL"
BASE COUNT          480 a          838 c          959 g          537 t
ORIGIN      
        1 accgcgccga gatccgggcc aatctcgccc gcgcactcgg catcattccc gatctggtgt
       61 cagtgaaggc gaccaccacc gacgggttgg gcttcatcgg gaggggtgag ggcttggccg
      121 ccgtcgctgt ggtcacggtc acccctctgt cgtaggattc gcccatgctc cgtctgtaca
      181 gcacgcttca ccgcgccgtg gtcccattcg aggcacggga cgaaggcgag gtcgcgatgt
      241 acctgtgcgg gccgaccgtg caaagcgagc ctcatgtcgg ccatgggcga tcggccgtgg
      301 ccttcgatgt cattcgccgc tatctgatgt ggagcggcca ccgggttacc tacgtccgca
      361 acgtgaccga catcgaggac aagatcatcg cggccgcagc cgagaccggg gagagcgtcg
      421 aagtcttagc ggagcggatg gcggcgcgat tcgcctccgg ttaccgggcg ctaggtgttc
      481 ttccccccga cctggagcca aaggcgaccg agcacatacc cgagatcatc gacctgatcc
      541 gggtgttgat cgaccgcggc ctcgcctacg catcggaggg agacgtctac ttctccgtca
      601 gggccgatga gagctatggg aagctgtcag gccggaaccc tgacgagctt cgttccggcg
      661 tccggatcga ggtgggggag gcgaagcacg atccgctcga cttcgccctc tggaaggcgg
      721 cgaaacccgg cgagccatgg tgggactcgc cctggggccc gggccgtccg ggttggcata
      781 tcgagtgctc ggccatggca accaggtacc tgggaaatga cttcgacatt cacggcggcg
      841 gcactgacct gatcttcccg catcacgaga acgagatcgc ccagagcgag ggggcttccg
      901 gtctgacctt cgccaggtat tggcttcaca acggaatggt caacctcgga ggggagaaga
      961 tggcgaagtc gaccgggatc ctggtcgacc tggcgtccat caccgagaaa cacggtggcc
     1021 gtgctctccg gcttctcctc ctgcgagccc attatcggtc tcctatcgag tactccgacg
     1081 agctcctcac cgagtcggag gatgcactgg accggctgca acgtttccgc gatagggcct
     1141 cgtcaggcga tccggatccg gaagccatcg accgttttcg ggaagtcatg gaggatgatt
     1201 tcggcacgcc gttggccgtc tctctgcttt tcgatctcgt tcgtgacggc aatcgcctcc
     1261 tcgacgaagg tggggatgcg gccgcaattg ccggcgcggt ggagtcgatc gtcggcgtgc
     1321 tcggcctcga tgacgccagc gtcccggccg aggagagcct cgatctgggc gatctccctg
     1381 gccggttcgg tctcgagggc aacggccacg acgtggtgga ccgactcgtc gagcttcggg
     1441 cggcggcacg ggaagaacgc aggttcgaag atgctgacgc catccgggat ggcctggaga
     1501 aggcgggggt gaccttggag gatggcccgg atggcacccg ctggctacgg aaatagggtc
     1561 gagggcatcc attcggtcgc tgcggccgct tcccggggcc gggttcgcaa gctctgggtg
     1621 gaggagaaga ggctcggtcg ggccgagatc gccgaaattg tcgaggccgt gggccgcgag
     1681 aggaccgcgt tgctccgtga cgtgaggcac atggcggaaa ccgaggcgcc tcagggggtt
     1741 gtcgccgagt gtgatcccat catgccggcc ccggtcgacg acttggcagg ccccggagcc
     1801 gctctggtcg tcctcgacca catcgaggat ccccacaatg tgggcgccgt cgcccggagt
     1861 gcactttccg cgggaatgac aggcatggtg gtgtcatcgc ggcgggccgc gccgttgtcg
     1921 gcagcggcgt tcaaggcggc tgcaggcgcc ctggagaccc tttcggtgtc catcgtcggt
     1981 tccatacctg aagcgctgag ccgactcaag gatcgggggg tatggatcgt tgggctcgag
     2041 gcgagctcgg ctcggtcttt gttcggcctg gatctgctca ccgagtcggt agccgtcgtc
     2101 gtcgggggag agggggccgg gctcagcgtc ctcagcgcca agcggtgtga cgtgctggcc
     2161 tcgattccga tggcatcgaa tttcgagagc ctcaacgctt ccgtctcggc ggcgctggcc
     2221 tgtttcgaga tcatgcgagt gcgggctgga gccgacccct ccctacaatc cgatctcccg
     2281 ccgggttagc tcagtcggta gagcagctgt cttgtaaaca gcaggtcatc ggttcgattc
     2341 cggtacccgg ctccaagaag tagtcgagtt ttctcagtta ctacctagta aaaccccgag
     2401 gactgtggga aatgaagggc gcctttcccg agttgcccct catttccccg catcttggaa
     2461 cacggatgtg acacgagaac ggctcagatg gcggtggatg gtgaccttca atcgggttcg
     2521 ggtgcgcgtt tggcgggtaa acggacgcat tgccgatgct ggaggaagcg tggaacagag
     2581 cctcgaccgt ccgatggaag cttggtccga cgacgaagta ctggagcaat atcgctccat
     2641 gactctcaac atgtctaact ctgaaggcat gtccaggctc gacctctctg cgatcactga
     2701 ggaaatgctt cgccggggtt tgtcgtttcc cgacgtttcg gctgcgactc gagtaacgga
     2761 taccgtcgac tggagtggtg aaggtggtgg agaggatccc gggtcgggcg ctct
//
LOCUS       DASTXO010000147         2340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_856521, whole genome shotgun sequence.
ACCESSION   DASTXO010000147 DASTXO010000000
VERSION     DASTXO010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856521"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..1400
                     /locus_tag="VFU96_03150"
     CDS             261..1400
                     /locus_tag="VFU96_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018344987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5112286.1"
                     /translation="MTRPVPLVTGATTSGVAVRVSAVFLVLILALGAGACPAVGQTSS
                     RGTSGSYQFHDVHFHLTNYIQEGIDARAFLGIMGRRAGRSTLFGIPLQQQWSHRISGD
                     NAPTYYLDTDARLYYYSFTDAYIAMQYKALSKAEQARFDPMITGFNPTDMYAADHIRR
                     VLLTFPGVFTGIGEFTIHKEFVSPKIAGDVASLTDPALDRVLEFAAEVGLVVLIHNDV
                     DRPFPRRGDPPVFLQQLRELLARHPDNSIIWAHTGLGRIIQPVENHLAMLESILEDPR
                     CSRVCFDISWDEVAKYVVSSPEATRRTAELLSSYPDRFLFGSDTVAPKSPEAYYSVYD
                     MYQPLWDLLSKEVREKVLKGNYERIFDEGRRKTRAWETAQLARRK"
     gene            1462..>2340
                     /gene="glsA"
                     /locus_tag="VFU96_03155"
     CDS             1462..>2340
                     /gene="glsA"
                     /locus_tag="VFU96_03155"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008928813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEU5112287.1"
                     /translation="MAVLALAVFTAGAQAQSKTKAAAANPYQGALDAAYAKFKDLQEG
                     KNPDYIPALAKVDPNIFGIALVTTDGKVYTTGDITSEVSIQSISKVFTMAKVIEEQGA
                     DAIEKNMGVDATGQVFNSIVAVEQYRGSEMNAMVNPGAITATSMVSGASREEIWNKIL
                     STHSDFAGRPLTVNQEVFKSEAETNQRNQAISMLMYAYGHIKSEPLRACDIYTEQCAI
                     SVNAKDLATMAATLANGGKNPVTGKVCMKAENVPKVLAVMATAGLYDDSGKWLYTTGL
                     PAKSGVGGGILAVSPGK"
BASE COUNT          455 a          758 c          738 g          389 t
ORIGIN      
        1 cgcacaacac ccttgatgtg gaaaacaaag ctccgacatt tcggaggtgg tccgatccgt
       61 cggagcggcg ggccccgcat gagtggccta ttctcagaga gactgtggat ctaatccgat
      121 tattggtaac gcattgcgac ctcatcccgt ggccgaagcg cgcggcacat tacatgcagt
      181 ttggcgcgtt gaagaccgag tcggccggat ggagatgtcc aagccggcct taagccgttt
      241 gtccttaagg gggaatcccg atgacccggc cggtgccact cgtgactggc gcaacgacgt
      301 ccggcgtcgc agttcgcgtc tcggcggtgt tcctggtcct catcctggct ctgggtgccg
      361 gtgcttgccc ggcggtcggg cagacgtcaa gccgggggac ttccggctcc taccagttcc
      421 acgatgttca cttccacctg accaactaca tccaggaggg gatcgacgct cgcgccttcc
      481 tcggcatcat gggaaggcgt gcagggcgct cgaccctgtt cgggatcccg ctccagcagc
      541 agtggtccca ccgcatctcg ggcgacaacg cgccgaccta ttacctcgat accgacgccc
      601 ggctctacta ctactcgttc accgacgcct acatcgcgat gcagtacaag gcactcagca
      661 aggccgagca ggcgcgcttc gaccccatga tcacggggtt caatcccacg gatatgtacg
      721 cggccgacca catccgccgc gtgttgctca cgttcccggg ggtgttcacc gggatcgggg
      781 agttcacgat ccacaaggag ttcgtgtcgc ccaagatcgc cggggacgtc gcgagcctca
      841 ccgatccggc cctcgaccgg gtgctcgagt tcgcggccga ggtgggcctg gtggtgttga
      901 tccacaacga cgtggatcgc ccgttcccgc ggcgcggcga cccgccagtg ttcctccagc
      961 agctccggga gctgctcgcg cgccaccccg acaactcgat catctgggca cacaccggac
     1021 tcgggcgcat catccagccc gtcgagaacc acctggccat gctcgagagc attctcgagg
     1081 acccccggtg ctccagggtc tgcttcgaca tctcgtggga cgaggtcgcc aagtacgtgg
     1141 tttcgagccc ggaggcgacg cgacgcaccg ccgagctgct cagcagttac ccggatcgct
     1201 tcctgttcgg atcggacacc gtggccccca agtcgccgga ggcgtactac agcgtctacg
     1261 acatgtatca gcccctctgg gacctgctgt cgaaggaggt gcgggaaaag gtcctcaaag
     1321 ggaactacga gcggatcttc gatgaagggc gtcgcaagac ccgagcatgg gaaaccgcgc
     1381 agctcgccag gcgcaagtga tgtcgactca ccccggaggt ggaaagtgaa ggcacttaga
     1441 agcactcgtt ggactctgct cgtggccgtg ctcgccctgg cggtctttac cgcgggggcg
     1501 caggcgcagt cgaagacaaa ggccgcggcc gcgaatccct accagggtgc gttggacgcg
     1561 gcctacgcca aattcaagga cctgcaggaa ggaaagaacc ccgactacat tccggcgctg
     1621 gccaaggtcg atccgaacat cttcgggatc gcgctggtga cgaccgacgg caaggtctac
     1681 accacggggg acatcacctc ggaggtctcg atccagtcca tctccaaggt cttcaccatg
     1741 gccaaggtca tcgaggagca gggcgccgac gcgatcgaga agaacatggg cgtggacgcg
     1801 accggccagg tcttcaactc catcgtcgcg gtggagcagt accggggctc cgagatgaac
     1861 gcgatggtga accccggggc catcaccgcc accagcatgg tgtccggagc gagccgtgag
     1921 gagatctgga acaagatcct cagcacccac agcgacttcg cgggtcggcc actgaccgtc
     1981 aaccaggagg tcttcaagtc cgaggccgag accaaccagc ggaaccaggc gatctccatg
     2041 ctcatgtacg cctatggcca catcaagtct gagcctcttc gcgcctgcga catctacacc
     2101 gagcagtgcg cgatcagcgt gaacgcgaag gatctggcga ccatggcggc caccctggcg
     2161 aacggcggca agaacccggt caccggcaag gtgtgcatga aggccgagaa cgtccccaag
     2221 gtgctggcgg tgatggccac ggccggcctc tacgacgact cgggcaagtg gctgtacacg
     2281 accggcttgc cggccaagag cggcgtgggc ggaggaatcc tggcggtgtc cccgggcaag
//
LOCUS       DASTXO010000148         4874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1335531, whole genome shotgun sequence.
ACCESSION   DASTXO010000148 DASTXO010000000
VERSION     DASTXO010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335531"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VFU96_03160"
     CDS             <1..322
                     /locus_tag="VFU96_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEU5112288.1"
                     /translation="LDTHYPFRVNRYTLRRGSGGQGRFPGGEGIEREIVFLADATLSL
                     MGERRRHRPWGLAGGEPGATGEDWLISSSGSRERLPAKCTVPVHAGDRLLVLTPGGGG
                     WGQT"
     gene            348..1772
                     /locus_tag="VFU96_03165"
     CDS             348..1772
                     /locus_tag="VFU96_03165"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEU5112289.1"
                     /translation="MSLVGDLRGVLSDDRVLEEPVELYLYSRDASLMQGQATCVVFPH
                     TTEEVAEVVVVAEKHGVPIVARGAGTGLAAGAIPVDGGIVLSTTAMNEIELDVENRTA
                     WVGAGVINLDLSKEAEPFGLCFAPDPSSQSACTIGGNVANNSGGAHCLAEGATTSHVL
                     GLEVVLAGGETLVLGGQAPDMPGLDLKGLLVGSEGTLGVVTRALVRLLPIEADVRTLL
                     LDFEDLEGATRTVSEVIASGLVPAALEIMDQKMTIAVENWLRAGLPCDCAAILLAEVV
                     GEPEAVEAEAALISRIGLANGARDVQVARDEAERAILWKGRKSAFGAVAQVSPNYYLH
                     DTVVPRSRLVETMGEIQAIADRYHLDVYNVFHAGDGNLHPLMAFDASDPVMMDKVHRA
                     GEEMVAACLSRGGALSGEHGIGLEKRDMMRADFTELDLDAQARLKEAFDPAGIFNPAK
                     VLPEGSRCFDFGGIRREIPDGAWV"
     gene            1769..2830
                     /locus_tag="VFU96_03170"
     CDS             1769..2830
                     /locus_tag="VFU96_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEU5112290.1"
                     /translation="MTGAAGGGPERVAPSSVEELARILKRATEEQRLVNVVGGGTRQG
                     YGSPPPAGLVVEMNRLAGIEEWDPDDLTVTVGAGTPVADLEATLAERAQTAVLPEVPG
                     ASTVGGVISAGVSSLRRARLLGTRERMLEVRLVTGDGRVVRGGGRVVKNVSGYDLPRL
                     VVGAFGSLGVITSVCLKLWPRPPASATVVIDDLEISKRVKRPLAVLEKNGVVEVFLWG
                     TPEEVIASTGRLGGEVTEGHRWPADPVGRFRWSVRVPPSLTSTAIDRLPDGWDYLAIH
                     GAGDIRAASENVDGADGLRMWVEGSGGALVATEAPVGGLDGLDPWGSPPPGMEIQRRL
                     IAQFDPGRVLNRGRLPGGL"
     gene            2830..4083
                     /locus_tag="VFU96_03175"
     CDS             2830..4083
                     /locus_tag="VFU96_03175"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEU5112291.1"
                     /translation="MGWVTPDHPTTDELNACVQCGLCLPACPTFRLTGRETASPRGRI
                     QAMKAVHNGILELDEPFQGVIDFCLGCRACEPVCPGMVPYGRALEGTRAEIAAQRPTF
                     GHRLRSFLLGTVIGSRRSLRFGSGALRVAQATKATSLAPTRYRRLSEGVRPLGARPKP
                     TLGHEGGSGVSGAIALLAGCIQDEWFRPVNHAAIELLEMAGFRVEVPRGQTCCGALAA
                     HDGKADAARALAERNMPVLSGYDQVVVTSAGCSAHVREYSHWAEGGDEVGNRAIDVTV
                     AVARAIEAGLLPVLPGGSGTIAIQDPCHLRHAQRVTLEPRRILEAGGYEVVEIDPAGM
                     CCGAAGLYTVLQPEASQELGNRKATQIRSTGVFRVASANPGCEMQLRASLGRDHEIAH
                     PIEWYLRAIAAAGPTGRVAAVKAER"
     gene            4080..4670
                     /locus_tag="VFU96_03180"
     CDS             4080..4670
                     /locus_tag="VFU96_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112292.1"
                     /translation="MTEDIARVDSRAAAKRYAILAYGSFGTMLDVGLMVIGSLLVGLA
                     VSVVLAGFDIVNVVQDLSTGAMLASSLVLAVIGLFCLGVAAEGPLGRGRRLVGFKLWE
                     IGLGRIVAVFVVGLLFLLVHNLVDGAVEGLPSPVTTGVAGIRAVGVAGMTVMPLLGVP
                     IALAARWYPDGPDWLRSTEIPIMFVVWTVATMAIVA"
BASE COUNT          742 a         1523 c         1730 g          879 t
ORIGIN      
        1 gctggacacc cactatccgt ttcgggtgaa tcgctacacg ctccgccggg gaagtggtgg
       61 gcagggccgg ttcccgggtg gcgagggaat cgagcgggag atcgtgttcc tggcggatgc
      121 gacgctctcc ctcatgggtg agcggaggcg tcatcgccct tggggtctgg cggggggaga
      181 gcccggggca actggcgagg actggctcat ctcatccagt ggaagtcgtg aacgtcttcc
      241 tgccaaatgc acggttcccg tgcacgctgg tgatcgcctc ctggttctca ccccgggagg
      301 gggtgggtgg gggcaaacct gaatcccgcc acaactaccc tcatcacgtg agtcttgtcg
      361 gggatcttcg cggtgtcttg agtgatgacc gggtgctgga agagccggtc gagctctatc
      421 tctactcgag ggacgcttcc ttgatgcagg gtcaggcgac ttgtgtcgtg ttcccacata
      481 ccaccgagga agtagccgag gtggtcgtgg tggcggagaa gcacggagtc ccgatcgttg
      541 cccgtggtgc gggtacgggc cttgccgccg gggccatacc ggtcgacggg gggatcgtgc
      601 tgtccaccac tgcgatgaac gagatcgagc tggatgtgga gaaccgaact gcgtgggtcg
      661 gcgcgggcgt gatcaacctc gacctgagca aggaagccga gccgtttggc ctctgtttcg
      721 ctcccgatcc ctcctcacag tcagcatgca ccatcggtgg caatgtcgcc aacaactcgg
      781 gcggagccca ttgtctggcc gaaggcgcca ctaccagtca tgtcctcggc ctcgaggtgg
      841 tcctcgcggg tggggagacg ctcgttctcg gtggtcaggc gcccgacatg ccgggtctgg
      901 acctgaaggg gttgctggtg ggctccgagg ggacgctcgg tgtcgtgacg agggccctgg
      961 tccgcctcct accgatcgaa gccgacgtga gaactctcct tctcgacttc gaggacctcg
     1021 agggggcgac gcggaccgta tccgaggtca tcgcgtcggg gctggttccc gcggccttgg
     1081 agatcatgga tcagaagatg accatcgccg tggagaactg gctgagggcc ggccttcctt
     1141 gcgattgtgc cgcgatcctc ctcgccgaag tggtggggga gcccgaggcg gtggaggcgg
     1201 aagcggcact gatctcacgc atcggccttg ccaacggagc acgtgatgtc caggtcgcca
     1261 gggacgaagc cgagcgggcc atcctctgga agggccgcaa atccgccttc ggggccgtgg
     1321 cccaggtttc ccccaactac tacctacacg acaccgtggt gcctcggtct cgtctcgtcg
     1381 agacgatggg agagatccag gcgatcgccg accgctacca cctggacgtc tacaacgtct
     1441 tccacgctgg ggatggcaat ctccacccgc tcatggcctt cgacgcttcc gatccggtga
     1501 tgatggacaa ggtccatcgg gccggggagg agatggttgc cgcatgtctc agtcggggtg
     1561 gcgccctgtc gggtgagcat gggatcgggc tggagaaaag agacatgatg cgggccgatt
     1621 tcaccgagct cgacctcgac gcccaggccc ggctgaaaga agctttcgac cctgccggca
     1681 tcttcaaccc ggcgaaagtg ctccccgagg gctctagatg cttcgacttc ggcgggatcc
     1741 ggcgggagat accggacggg gcctgggtat gaccggggcc gccgggggcg gtcccgagcg
     1801 tgtcgctccc tccagcgtcg aggaactggc ccggatcctc aagcgggcca ccgaggagca
     1861 acgacttgtg aatgtcgtcg ggggcggaac cagacagggc tacgggtcgc cgccaccggc
     1921 tggtctggtg gtcgagatga acaggcttgc cggcatcgag gaatgggacc ccgacgacct
     1981 gaccgtgaca gttggagccg ggactccggt ggccgacctg gaggcgacgc ttgccgaacg
     2041 ggcacagacc gcggtcctgc cggaggttcc cggcgcgtcc acggtcggtg gggtgatctc
     2101 ggccggtgtc tcctcgctgc gccgggcccg tctcctcggg accagggagc ggatgctcga
     2161 ggttcgtctg gtgaccggtg acggccgggt ggttcggggt ggagggcggg tggtcaagaa
     2221 cgtctccgga tacgacttgc cccgcttggt cgtgggtgcc tttggctccc tcggtgtcat
     2281 cacatctgtt tgcctgaagt tgtggccgag gcccccggcc tcggcgaccg tggtcatcga
     2341 tgacctcgag atttccaagc gcgtgaagcg gccgctcgcc gtcctcgaga agaacggcgt
     2401 ggtcgaggtc tttctgtggg gcacgccgga ggaggtcatt gcctccaccg gcagactcgg
     2461 gggcgaggtc accgaaggcc accgatggcc ggccgatccc gtgggacgat tccgatggtc
     2521 ggtgagagtc ccaccctccc tcacctcgac tgccatcgat cgacttcctg acgggtggga
     2581 ctacctggcc atccatggcg ccggcgacat tcgagccgct tcggagaatg tggatggggc
     2641 ggacggattg cggatgtggg tagagggctc tggcggcgcc ctggttgcga cggaggcgcc
     2701 tgtcggcggc ctcgatgggt tggatccctg gggttcgccg ccaccgggga tggagatcca
     2761 gcgtcgtctc atcgcgcaat tcgacccggg ccgcgtcctc aaccggggtc gtcttcctgg
     2821 agggctgtga tgggatgggt gaccccggac cacccgacga cagatgagct caacgcctgc
     2881 gttcagtgcg ggctgtgtct ccccgcctgt ccgactttcc gcctgaccgg gagggagacg
     2941 gcatctccgc gcgggcggat ccaggcgatg aaggcggttc acaatggcat cctcgagctg
     3001 gacgagcctt tccagggtgt gatcgacttc tgcctcgggt gtcgagcctg cgagccggtt
     3061 tgccccggaa tggtcccgta cggaagggcg ctcgagggaa cccgagccga gatcgccgcc
     3121 caacggccga ccttcggcca ccggcttcga tctttcttgc tcggcacggt cattggctcg
     3181 aggcggtcgc tccgcttcgg gtcgggtgct ctgcgagtcg cccaggcgac caaagccact
     3241 tccttggcgc cgacgcgcta tcggcggctt tccgaaggcg tgcgcccgct cggggcgcgg
     3301 cccaagccga ctctcggcca cgaaggtggc agcggcgtct cgggtgccat cgccttgctg
     3361 gccgggtgca tccaggatga atggttccgg ccggtcaatc acgccgccat cgaattgctg
     3421 gagatggccg ggtttcgcgt cgaggtgccg cgcggccaga cgtgctgcgg cgcactcgcg
     3481 gcacacgacg gcaaggcgga cgccgcccgc gccctggccg agcgcaacat gccggttctc
     3541 tccggctatg accaggtggt ggtcacctcg gccgggtgtt ccgcccatgt ccgtgaatac
     3601 agccactggg ccgaaggcgg ggacgaagtg ggcaaccggg cgatcgacgt cacggtggca
     3661 gtggcccgcg ccatcgaggc cggcctcctt cccgtccttc cgggagggtc ggggaccatc
     3721 gcgatccagg acccgtgtca cctgcggcat gcccagcgag tgacgctcga gccaaggcgc
     3781 atactcgagg cgggcggata cgaggtcgtc gagatcgatc cggcggggat gtgctgcggc
     3841 gcggccggcc tgtacacggt gctccagccc gaggcatcac aggaacttgg aaaccgtaaa
     3901 gcaacgcaga tacgttcaac cggtgtgttc agggtcgctt ccgccaaccc cggctgtgag
     3961 atgcagctca gggcgtcgct gggccgcgat cacgagatcg cgcacccgat cgagtggtat
     4021 ctgcgcgcca tcgcggcagc cgggccaacc ggtagggtcg ccgcggtgaa ggcagagaga
     4081 tgaccgagga catcgcccgc gtcgacagcc gcgccgcggc caagcgatat gcgatcctgg
     4141 cctacgggtc gttcgggacg atgctcgacg tggggctgat ggtgatcggg agcctgctcg
     4201 tcggcctcgc ggtctcggtc gtgctggccg gtttcgacat cgtcaacgtg gtccaggacc
     4261 tttccaccgg ggccatgctg gcctcgtcgc tcgtccttgc ggtgatcggc ctgttctgcc
     4321 tgggtgtagc ggcggagggc ccattgggaa ggggccggcg ccttgtcggc ttcaagctct
     4381 gggagatcgg cttgggccgg atcgtcgccg ttttcgtcgt gggcctcctg ttcctgctcg
     4441 tccacaacct ggtcgatggc gcggtcgagg gactgccgtc gccggtgacc accggggttg
     4501 ccgggataag ggcagttggc gtcgccggga tgaccgtcat gccactcctc ggcgtcccga
     4561 tcgccctcgc cgcacgatgg tatcccgatg gccccgactg gctccgctcc accgagatcc
     4621 ccatcatgtt cgtggtctgg accgtggcca cgatggccat cgtggcctga gccttcctcc
     4681 cccagggggt cggggaggtt gcccggcagg gccggggcag gtcagagagc cttcgtgaag
     4741 atctgggccg ggtggacctc gaagccttct cgctgcaagg cctcgacgct ccagacagtg
     4801 atcgggatca tcgcctcgac ccgacttccg aagcgatctc gcaacaggtc tcgcagcccc
     4861 tgaaagagca ttgg
//
LOCUS       DASTXO010000149         2901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_451024, whole genome shotgun sequence.
ACCESSION   DASTXO010000149 DASTXO010000000
VERSION     DASTXO010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451024"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..469)
                     /locus_tag="VFU96_03185"
     CDS             complement(80..469)
                     /locus_tag="VFU96_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112293.1"
                     /translation="MTDQGDKTTEAGESKQQRWFAEVQEALDRTGDALRTAWEATRDS
                     RMSALESAKQAAQELGEVIDQGIAAAKERWAAADQAEETGGTAETETPFAEEATAAET
                     VPNEAEPIPMSGDVTVTPVESDTDEKA"
     gene            561..1040
                     /locus_tag="VFU96_03190"
     CDS             561..1040
                     /locus_tag="VFU96_03190"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU5112294.1"
                     /translation="MDGYTARPVSMTAHIPAPPDQVFSLVSDTRNDPLWCQNVETVDL
                     VEGSNIEVGTRFRFHQHLDRPGAERVQFDVDVEVIEMGDRSITWVARDRFQTRAISLA
                     VEPEGTGTRITQVTRATFLRPPGMAKWLYPLLARRIFSGQFRDLAAYFEQNRANPAE"
     gene            complement(1024..1680)
                     /locus_tag="VFU96_03195"
     CDS             complement(1024..1680)
                     /locus_tag="VFU96_03195"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEU5112295.1"
                     /translation="MSSRIRASLAVLVAATLCASCIRPGVEGSGDVITEPREVSGFNE
                     IVLSGSGRVVVEVTGTESLTIEAEDNIMPFLETTVRNGRLRLDTNRSISPTVEIVYTI
                     TAATLDGLVISGSGIVEADAIDGTDFRVDISGSGDVDAEGTLSGLLAISISGSGEFDG
                     ESLTAPEGTVDVSGSGNAVVNVTADLEVSVSGSGDVEYLGAPHVDKDVSGSGAVTQRD
                     "
     gene            1736..1930
                     /locus_tag="VFU96_03200"
     CDS             1736..1930
                     /locus_tag="VFU96_03200"
                     /inference="COORDINATES: protein motif:HMM:NF020908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HEU5112296.1"
                     /translation="MEDRRPSDPYESLPERLIREAIEAGEFDDLPGAGKPIPGAGVPD
                     DDMWWVREWMKRNRAEPDEG"
     gene            1930..>2901
                     /locus_tag="VFU96_03205"
     CDS             1930..>2901
                     /locus_tag="VFU96_03205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005248615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5112297.1"
                     /translation="MELGITTFAETYPDPKTGEMIGHGERLRNVLEEIVLAEQVGLDV
                     YGVGEHHRPDFASSSPAVVLAAAAGMTDRIRLTPAVVILSSDDPVRVFQDYATLDLVS
                     NGRAELLVGRGSFIESFPLFGYSLSDYDELFSEKLDLLLAIRHHERVTWSGRFRPPLQ
                     DQAIYPRPQQDPFPIWVGVGGSPDSIVRAATRGLPVALAIIGGNPDRFAFFADLHRRT
                     LVESGHDPAFAPLAVHAHGYVADSGEQASDEYYNAYAVAMTQLGRERGWGPMTRPQFE
                     MMLAPEGSLVLGDVETVAAKIIRMREVLGIDRFMLHISVGTLPHELVL"
BASE COUNT          469 a          998 c          883 g          551 t
ORIGIN      
        1 cccgaccgcg tgctcgaagc ccggcgtcgg gtggctcctt cccctagttg acgtccggac
       61 gcgtttcgac cgttgtggat caggcctttt cgtcggtgtc gctctcgacc ggcgtgaccg
      121 tcacgtcccc tgacatcgga atgggctcgg cctcgttcgg cacggtctcg gcggcggtcg
      181 cctcttcggc gaatggggtc tcggtctccg ccgtgcctcc ggtctcctcg gcctgatctg
      241 ccgccgccca gcgctccttg gcggcggcga tgccctggtc gatcacctca cccagctcct
      301 gcgcggcctg cttggccgac tcgagggcgc tcatgcggct gtcgcgggtc gcttcccagg
      361 ccgtgcggag ggcatctccg gtgcgatcga gcgcttcttg cacctcggcg aaccaacgct
      421 gttgcttgct ctcgccggcc tcggtcgtct tgtcgccttg gtccgtcatc acgtcctcct
      481 gggtcgtcgt ctccacattc tggtcccaat gcccgattga cgcgcaaacg tcgctcgggc
      541 gatacggata tcgtcctccc gtggacggct acaccgcccg gccggtctct atgacggcgc
      601 acatcccggc ccctcccgat caggtcttca gcctcgtctc ggacacgcgt aacgacccac
      661 tgtggtgcca aaacgtggaa acagtcgacc tggtcgaagg gagcaacatc gaggtgggga
      721 cccggtttcg cttccaccag catcttgatc gccccggggc ggagcgggtc cagttcgacg
      781 tcgacgtcga agtgatcgag atgggggacc gctcgatcac atgggtggcc cgcgaccgat
      841 tccagaccag agccatcagc ctcgcggtcg aaccggaggg tacgggcacc cgtatcaccc
      901 aggtcaccag agcaacgttt ctccgcccac cggggatggc caaatggctc tatccgttgc
      961 tcgctcgacg gatcttctca ggtcagttcc gtgacctcgc cgcctacttc gaacagaatc
     1021 gggctaatcc cgctgagtga ccgctcctga cccgctcacg tccttgtcaa cgtgcggtgc
     1081 tccgagatac tcgacatcac ccgacccgga gaccgacacc tcgaggtcgg cggtgacatt
     1141 gaccaccgca ttgcccgacc ctgagacgtc caccgtgcct tcaggcgcgg tcagcgactc
     1201 tccatcgaac tccccggatc cgctgatcga aatcgcgagc aggccgctga gggttccttc
     1261 ggcgtcgacg tcgcctgatc cactgatgtc gacccggaaa tccgtgccgt cgatggcgtc
     1321 ggcttcgacg atcccgcttc cgctgatgac gagcccgtcg agggtcgccg cggtgatcgt
     1381 gtagacgatc tcgaccgtcg gtgagatgct ccgattcgta tcgagccgaa gacgtccgtt
     1441 tctgactgtg gtctcgagga acggcatgat gttgtcttcg gcttcgatgg tcaacgattc
     1501 ggtcccggtg acctcgacga ccaccctccc gctgccgcta aggacgatct cgttgaaccc
     1561 gctcacctct ctcggctctg tgatgacgtc tccagacccc tcgacaccgg gtctgatgca
     1621 ggacgcgcag agcgtcgccg ccacgagcac ggccagtgag gcgcggatac ggctcgacac
     1681 gggccccacc ctaagccgac gaccccgatg agccgagccg ataacgtccc cggtcatgga
     1741 agaccgccgg ccgagcgatc catacgagtc acttccagag aggctcatcc gggaggcgat
     1801 cgaagccggc gagttcgacg acctccccgg tgccgggaag ccgataccag gtgcgggcgt
     1861 gcctgacgac gacatgtggt gggtgcgcga atggatgaag cgaaaccgag ccgaaccgga
     1921 tgaaggctga tggaactggg gataaccacc ttcgccgaga cctatccgga tccgaagaca
     1981 ggtgagatga tcggccacgg cgagcgtctc cgcaacgtcc tcgaggagat cgttctcgcc
     2041 gagcaggttg ggctcgatgt ctacggcgtt ggggaacatc accggcccga cttcgcctcg
     2101 tcttcacccg cggtcgtctt ggccgctgcc gccggcatga ccgaccggat caggttgacc
     2161 cccgccgtcg tcatcctctc ctctgacgat ccggtgcgtg tgttccagga ctacgccact
     2221 ctcgatctcg tctcgaatgg ccgggcagag ctgttggtcg gtcggggatc cttcatcgaa
     2281 tcctttccac tcttcggtta ctcgctctcc gattacgacg agctgttctc cgagaagctc
     2341 gacctgctgt tggcgatccg ccatcatgag cgggtcacgt ggtcggggag gttccgcccc
     2401 ccgctacagg accaggccat ctatcccagg ccacaacagg acccgttccc gatctgggtg
     2461 ggagtggggg gcagcccgga ttccatcgtc cgcgccgcca cacgagggct cccggtcgcc
     2521 ctggcgatca tcggaggcaa cccggaccgt ttcgccttct tcgccgacct ccatcgcagg
     2581 accctggtcg aatcgggaca cgatcccgcc ttcgcccccc tcgccgtcca tgcccacggc
     2641 tacgtcgcag atagcggcga gcaggcgtcc gatgagtact acaacgccta cgccgtggcc
     2701 atgacccaat tgggcagaga gcgtgggtgg gggcctatga cgcgcccgca gttcgagatg
     2761 atgcttgccc cggagggctc actcgtcctc ggagacgtgg agacggtcgc cgcgaagatc
     2821 atccgaatga gggaggtgct cgggatcgac cgtttcatgc tgcacatcag cgtcggcacc
     2881 ctgccccacg aactggtgct c
//
LOCUS       DASTXO010000150         8038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1556471, whole genome shotgun sequence.
ACCESSION   DASTXO010000150 DASTXO010000000
VERSION     DASTXO010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8038
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1556471"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VFU96_03210"
     CDS             <1..474
                     /locus_tag="VFU96_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112298.1"
                     /translation="LFLYEMGNEPAANALFAIWVLILMASIALAVLRYRLYDFDRVIS
                     RTASYAVLVLILGTVYVVGAVWLPTRIVGEQSPLFVAGSTLAVAALFTPIRRSVMAWV
                     DRRFYRSRYQMELVIHEFSSHLQDQVDPDQMASACVGLIADTLSPTTAGVWVKGG"
     gene            792..1805
                     /locus_tag="VFU96_03215"
     CDS             792..1805
                     /locus_tag="VFU96_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006379893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5112299.1"
                     /translation="MTNMIEASGLVKRYKEVTALAGLDLVVPEGVVLGLLGPNGAGKT
                     TAVSILSTLLEPDEGQAMVAGADVRKDPKAVRRLIGLSGQYAAVDEHLTGFENLDMIG
                     RLYHLGKARSRDRARELLEIFDLTEAGDRPVKTYSGGMRRRLDLAGALVAKPPVLFLD
                     EPSSGLDPRSRVDLWETIRDLVKEGATLLLTTQYLEEADQLADDIVVIDHGREIAHGS
                     ADELKTQVGGERIEVTVADLVHVTPAQEVLASFAVGEIRIDHHSRGLTAPIAGGAPVL
                     TQALRELDARGIVVQDVGLRRPTLDDVFLTLTGHVAEEELESNGGRSKKHKEEEELEE
                     VSR"
     gene            1802..2617
                     /locus_tag="VFU96_03220"
     CDS             1802..2617
                     /locus_tag="VFU96_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5112300.1"
                     /translation="MSQLMTALGDGAVVAKRNVIKIKRVPEILVFVLLSPIMFVLLFA
                     FVFGGAIDVATGAPPEVYREFLIAGILAQTVIFGATFTGAGLADDMQKGIIDRFRSLP
                     MARSAVLVGRTSSDVVYNALSVAIMALTGLLVGWGIRTSFLEAALGFGLLLLFAYAFS
                     WVMAYVGLLVPSVEVINNASFMVIFPITFVSNAFVPLETLPAPLQTFAEWNPVSSLTQ
                     AVRELFGNIPPGAPEPTAWPLQNPILYTLIWVVIIMAIFVPLSIRQYKRAARR"
     gene            2725..3180
                     /locus_tag="VFU96_03225"
     CDS             2725..3180
                     /locus_tag="VFU96_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HEU5112301.1"
                     /translation="MAEDVGFTEKFEKFSQRVTHAVGRPWAFLIALLVIVVWAVTGPL
                     FGFGDTWQLVINTGTTIVTFLMVFLIQQTQNKDSKAMELKLNELVAATKGASNRLIDV
                     EALTEDDLDVLHGYYRQLVDLSQRDHDLTESHSLEEAERLHGEKSGGKP"
     gene            3205..4335
                     /locus_tag="VFU96_03230"
     CDS             3205..4335
                     /locus_tag="VFU96_03230"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEU5112302.1"
                     /translation="MSTSQSTAEVILAAITEAVAPGTSLNDVLTKLAETAKQISGASY
                     AAIGIPEPEGDAFSAFIHVGMSDELVAEMGPLPRTHGMLDAMLHDPEPLRLPDVKQDR
                     RFRGWWPRAHPSMSSFLGVPIVSEGEVIGAFYLTDKIGAAEFTDGDQAAIALLASHAA
                     AAIEAAEMFEDSRTLAMSEERERLARELHDALNQSLFSLTLTAKAASRHLATDPDRAA
                     EELSEIASLSRQAMAELRAVVEGLRTPDVERDGLLPAIRNLADLLSRVHHIDIEVVSD
                     GDHALEGRAEHEVFRIIQEALTNAVRHSRAAKVTVSVSNGERLAVTIEDDGQGFEPDA
                     RMSRGRRLGLTSMRDRASVIGGRLTISSSPGEGATVRLEVPT"
     gene            4332..4976
                     /locus_tag="VFU96_03235"
     CDS             4332..4976
                     /locus_tag="VFU96_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5112303.1"
                     /translation="MSKSVKVMIVDDHPVVRKGLRAFLETQPGMEVVGEAADGQEAMR
                     QLATGEPDLVLLDLHLPDASGSELIPQMRKWNPAVKVLVLTGFADRREVVPAIRAGAD
                     GYLLKDTDPDQLADAIRELASGRTVYAQEAAAALSAYVSGGDPHEALSDLTAREREVL
                     AGLGQGWSNARLAEELFVSEKTVKTHVSAVLRKLGLDDRTLAALFAVKSGLVDG"
     gene            5068..5775
                     /locus_tag="VFU96_03240"
     CDS             5068..5775
                     /locus_tag="VFU96_03240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5112304.1"
                     /translation="MSTAIVTGASRGFGRALAVELARAGWSVVIDARDEPDLEAAASI
                     IEDAGSGDVIAIAGDVTDPEHATRLVEAAIAAGDFSLLVNNASLLGPSPQPALADYPV
                     DVLEGVIATNLLAPLRLIQLSLPHLRSVAGTVVNITSDAAVEGYEGWGGYGASKAGLE
                     QLSNVLASEETGVAVYWLDPGDMNTRMHQEAFPGDDISDRPLPEARVPALVRLIEGRY
                     PSGRYQAEDLLAKETSP"
     gene            5772..6827
                     /locus_tag="VFU96_03245"
     CDS             5772..6827
                     /locus_tag="VFU96_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019587227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEU5112305.1"
                     /translation="MTAPAPLRPADALDGFVLPLELEATRPPETRGVRRDQVRLMVSD
                     RNGIRHRVFRDLPSELRAGDLVVVNNSATLPASVVIDGDLVVHFSTELPGGLHVVELR
                     RLSGAGSLPHEDPRAGRLHLPGGGTIELLTPYPVGGGSHRLWVATASVGRPLVEYLET
                     WGRPIRYRHTDGPYPLDAYQTIFARVSGSAEMPSAGRPFTDRLVTALMTAGIAVAPLT
                     LHTGVSSLEAGEDPYPEWFEIPESTASLVNHTRANGGRVIAVGTTVVRALETTVDAKG
                     LSHPGRTWTDLVIGSDHALGVVDGLITGWHEPQSTHLDLLEAVAGRRRLAESYRNALE
                     QGYMWHEFGDSHLIVPG"
     gene            complement(6840..7079)
                     /locus_tag="VFU96_03250"
     CDS             complement(6840..7079)
                     /locus_tag="VFU96_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5112306.1"
                     /translation="MTDTVHNRIAMLRAERGVSRRQLSEALGVHYQTIGYLERGEYSP
                     SLFLALRIAEYFEVPIEVVFSTEPFARIGSNSATG"
     gene            complement(7076..7930)
                     /locus_tag="VFU96_03255"
     CDS             complement(7076..7930)
                     /locus_tag="VFU96_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014738682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5112307.1"
                     /translation="MSPTTNAIKSGFARGWTEFKLSLQSPQDQGFYLFMAALVLAYLW
                     FNRNTEVEGTNLSYPTVVLPSMLGALLVFSLIIGPAYALAMEREDGTLLRAKAIPHGV
                     VGHVTGHVILNILSIVPSFLIILIPGALLFDGLMQNGAEGWATVAWVTLLGLWATLPI
                     GIIIGSLAPSVQKVGTWGMFPVLVLVGISGIFFPIQDLWGWVQTVSQMFPMYWVGLGM
                     RSAFLPEAAAVLEIGGSWRTLQTVLVLSAWAVAGSLIAPVVVRRMARRQSGSQVEAAK
                     EQAVQWVR"
BASE COUNT         1425 a         2651 c         2585 g         1377 t
ORIGIN      
        1 ctcttcctgt atgagatggg caacgagccg gcggcaaatg ccctttttgc catctgggtg
       61 ttgatcctga tggcatcgat cgccctcgcc gttcttcgct accggcttta cgacttcgat
      121 cgcgtgatca gccggaccgc gtcgtatgcg gtgctcgtgc tgatcctcgg cacggtctac
      181 gtggtcgggg cagtctggct gccgacaaga atcgtcggcg agcaatcccc gctattcgta
      241 gctggatcga ccctggccgt ggcagctctc ttcactccca tccggaggag cgtgatggcg
      301 tgggtggacc gccgtttcta ccgctcccgg tatcagatgg agcttgtgat ccacgagttc
      361 tcctcccatc tccaagatca agtggaccca gaccagatgg cgtcggcctg cgtaggcctc
      421 atcgcggaca cgctcagccc gacgacagcc ggggtgtggg tcaagggcgg gtagcgccca
      481 gaccgcatcg acttacccgc tccccgcttc tcccgtccat ctgctcgaac cgagatcgtc
      541 atcaaaggtt cacccgcccc cggaaatacc cgggcgcccc aaccggttgg cacctaacgt
      601 gctcaggcgg tctaggacat tgcggaccga aactgtccgc attaccgcat agagtcggga
      661 agctacccca acgagacgtg tcttccaatc gtcctcaggg ccgatcggga ttcctactcc
      721 ggaccgatga gtttcgacgg cgacgccggt ctcattgaca gaagacctcg acacaggaag
      781 aggagaaacc catgaccaac atgatcgagg catcgggcct ggtcaagcgg tacaaggaag
      841 tcaccgcact ggccggtctc gacctcgtcg tccccgaggg cgtcgtgctt ggccttctcg
      901 ggccgaacgg cgccgggaag acgaccgcag tgtcgatcct gtccaccctg ctcgagccag
      961 acgagggcca ggcgatggtc gccggagccg acgtcaggaa agaccccaag gcagtccgcc
     1021 ggctcatcgg gctctccggc caatacgccg cggtcgacga gcacctcacc gggttcgaga
     1081 acctggacat gatcggacgt ctatatcacc tgggaaaggc gaggtcgcgc gaccgcgcca
     1141 gggagctgct ggagatattc gacctcaccg aagccggtga ccggccggtg aagacttatt
     1201 cgggtggcat gcgccgccga ctcgacctgg ccggcgcctt ggtcgccaaa ccaccggtgc
     1261 tgttcctcga tgagccgtca tcgggtctcg acccgagaag ccgggtagat ctgtgggaaa
     1321 cgatccggga cctggtcaaa gagggggcga cccttcttct gaccacgcag tacctggagg
     1381 aagccgacca gctcgccgac gacatcgtgg tcatcgatca cgggcgtgag atcgcccatg
     1441 gctccgctga cgagctcaag acccaggtgg gtggcgagcg gatcgaggtc acggttgccg
     1501 atctcgttca cgtgacaccg gctcaggaag tcctggcatc gttcgccgtt ggcgagatcc
     1561 ggatcgatca tcacagtcgc gggctcacgg ccccgattgc cggcggggca cctgtcctca
     1621 cccaggcact acgcgagctc gatgcgcgcg ggatcgtggt gcaggacgtg ggtctccgtc
     1681 ggccgacact cgacgacgtg ttcctcaccc tgaccggtca cgtcgccgag gaggagttgg
     1741 agtccaacgg cggaagatcg aagaagcaca aggaagaaga agagctggag gaggtcagcc
     1801 gatgagccag ctgatgactg cccttggcga cggcgccgtg gttgccaagc gaaatgtgat
     1861 caagatcaag cgggtccctg agatcctggt gttcgtcctg ctctccccga tcatgttcgt
     1921 gctgctgttc gccttcgtgt tcggtggcgc tatcgacgtg gcgaccgggg ccccacccga
     1981 ggtctatcgc gagttcctga ttgccgggat cctggcgcaa accgtgatat tcggggcgac
     2041 cttcaccggc gccgggctgg ccgacgacat gcagaagggg atcatcgacc ggttccggtc
     2101 gctccccatg gcgagatctg cagtcctcgt cggtcggaca tcgagcgatg tcgtctacaa
     2161 cgcactgtcc gtggcgatca tggccctcac cggcctcctg gtcggatggg ggatccggac
     2221 gtcgttcctc gaggcggcgc tggggttcgg gctcctgctc ctgtttgcct acgccttttc
     2281 ctgggtgatg gcatatgtcg ggctgctggt cccgagcgtg gaagtgatca acaacgcgtc
     2341 gttcatggtg atcttcccga tcaccttcgt gtccaacgcc ttcgtcccgc tcgagaccct
     2401 gcccgctccc ctgcagacct tcgccgaatg gaatccggtg tcgtcgttga cccaggcggt
     2461 gcgtgagctg ttcggcaaca tcccacccgg ggctcccgag ccgacggcct ggccattgca
     2521 gaacccgatc ctctacacct tgatctgggt cgtgatcatc atggccatct tcgtcccgct
     2581 ctcgatccgg cagtacaagc gggctgcccg ccgctgacgg cgaatcgcca ggtccctcca
     2641 cgggtcaaca cccggtcccc gaccgggtgt tgactctggg ttgaatgacc gccatcgaag
     2701 gcggccgtcg agcaggaggc accaatggcg gaagatgttg ggttcaccga gaaattcgag
     2761 aagttctcac aacgagtgac ccacgcggtg gggagaccct gggcgtttct gatcgcgctg
     2821 ctggtcatcg tcgtctgggc ggtaaccggg ccgctgttcg gattcgggga cacctggcaa
     2881 ctggtgatca ataccgggac caccatcgtc accttcctca tggtgttcct gatccagcag
     2941 acccagaaca aggacagcaa ggcgatggag ctcaagctca acgagctggt ggcggcgacc
     3001 aagggcgcca gcaaccggct gatcgacgtc gaggcgctca ccgaggacga tctcgatgtc
     3061 ctccatggct attaccgaca actcgtggac ctgtcacagc gcgatcacga cctcactgag
     3121 tcgcactccc tggaggaggc ggaacggctg cacggcgaga agagcggcgg aaagccgtag
     3181 aagggccgcc ggtagccttc gaaggtgagc acgtcacaga gcaccgccga agtgatcctc
     3241 gccgcgatca cggaggcggt cgctccgggc acgtctctga acgacgttct caccaagctg
     3301 gcggagaccg ccaaacagat ctccggcgcc tcctacgccg ccatcgggat ccccgagccg
     3361 gaaggcgacg cattctccgc cttcatccac gtcggtatga gcgacgagct ggtcgccgag
     3421 atgggcccgc tgccacggac ccatgggatg ctcgatgcga tgctccacga tccggagccg
     3481 ctgcgcctcc ccgacgtcaa acaggaccgt cgttttcggg gctggtggcc ccgggcccat
     3541 ccttcgatga gctcattcct cggcgtcccg atcgtgagcg aaggcgaagt catcggcgcg
     3601 ttctacctga ccgacaagat cggggcggcg gaattcaccg acggcgacca ggccgccatc
     3661 gctcttctcg cctcgcacgc cgccgccgcc atcgaggccg ccgagatgtt cgaggactcc
     3721 cggaccctgg ccatgtccga ggagcgggaa cgtcttgccc gcgagctcca cgacgccctg
     3781 aatcaatcgc tgttcagcct cacactcacc gccaaggccg cctcgcggca cctggccacc
     3841 gacccggacc gcgccgccga ggagctgagc gagatcgcat ccctatcgag acaggcgatg
     3901 gccgagctgc gagcggtggt cgaagggctg aggactccgg atgtagaacg cgatggcctt
     3961 ctccctgcga tacggaacct cgccgacctg ctcagccggg tgcatcacat cgacatcgag
     4021 gtggtctcgg acggtgacca cgccctggag gggcgcgccg agcatgaggt attccgcatc
     4081 atccaggagg ccctcaccaa cgccgtccgg cattcccggg ccgccaaggt cacggtgtcg
     4141 gtgtccaacg gagagcggtt ggcggtgacg atcgaggacg atggccaggg gttcgagccc
     4201 gacgcacgga tgagccgtgg ccgacgtctc ggcctcacct ccatgcggga tcgtgcctcg
     4261 gtcatcgggg gtcgtctgac catctcctcg tcgcccgggg agggagcaac cgttcgtctc
     4321 gaggtcccga cttgagcaaa tcggtgaagg tgatgatcgt cgatgatcac ccggtcgtgc
     4381 gcaaggggct ccgtgccttt ctcgagacgc aaccggggat ggaagtggtg ggcgaagccg
     4441 ccgacggcca ggaggcgatg cgccagctgg caaccggtga gcccgacctg gtcctgctcg
     4501 atctgcacct tcccgatgcc tccgggtcgg agttgatccc tcagatgaga aaatggaacc
     4561 cggcggtgaa ggtcctcgtg ctcaccggct tcgccgacag gcgcgaagtg gttccggcga
     4621 tcagggccgg ggcggacggc tatctgctca aggacaccga ccccgaccag ctcgccgacg
     4681 cgatcaggga actggccagt ggccggacgg tttacgccca ggaagcggcc gccgcgctct
     4741 ctgcctacgt ttcgggcggc gatccccacg aggctctgtc cgacctcact gcccgggaaa
     4801 gggaggtctt ggcgggtctc gggcagggtt ggtccaacgc ccggctcgcc gaggagctct
     4861 tcgtgtctga gaagacggtc aagactcacg tctcggccgt gctacggaag ctcggtctcg
     4921 acgaccggac tctagccgca ctattcgcag tcaagagtgg gttggtggac ggttgattcg
     4981 ccttccggcg cctccgtccc gagacctagg gcgaatcggg ccggggacgg atgaccgcga
     5041 gtccgtccgg ccgtaccgtc tacagggatg agcaccgcaa tcgtcaccgg agcgtcccgt
     5101 gggttcgggc gcgcccttgc cgtcgagctg gcacgtgccg gttggagtgt ggtgatcgac
     5161 gcccgggacg agcccgacct cgaggcggcc gcctcgatca tcgaagacgc cggctccggt
     5221 gacgtcatcg ccatcgccgg tgacgtgacc gacccggagc acgcgacccg cctcgtggaa
     5281 gccgcgatcg cagcgggcga cttctcactg ctggtcaaca atgccagcct gctcggaccg
     5341 agcccgcagc ctgccctggc ggattacccg gtcgatgtcc tggaaggggt catcgccacc
     5401 aatctcctgg ccccgcttcg cctcatccag ctctcccttc cccacctccg ctctgtcgca
     5461 gggacggtgg tgaacatcac ctccgatgct gctgtcgagg gctacgaggg gtggggcggg
     5521 tatggggcat cgaaagccgg cctcgaacag ctctccaacg tcctggcttc cgaggagacc
     5581 ggcgtggccg tctactggct cgaccccggc gacatgaaca cgaggatgca ccaggaggcg
     5641 ttcccgggcg atgacatctc ggaccgaccc ctgcccgaag ccagggttcc ggcgctggtt
     5701 cgcctcatcg aaggccgata tccgagcggc cgttaccagg ccgaggacct cctggccaag
     5761 gaaaccagcc catgaccgcc ccggcgccat tgcggccggc tgatgccctc gatgggttcg
     5821 tccttccgct ggagctggag gcgacaaggc cgcctgagac ccgcggtgtc agacgagacc
     5881 aggtgcgcct catggtgtca gacagaaacg gcatacggca ccgagtcttt cgcgacctac
     5941 cctccgagct gagggccggg gacctcgtcg tggtcaacaa ctcggccacc ctgccggcat
     6001 cggttgtcat cgacggcgac ctcgtggttc atttctcgac cgagctccct ggaggcctcc
     6061 atgtggtcga gctgcgacgt ctgtcggggg caggctcgct tcctcacgaa gacccccgag
     6121 ccgggaggct gcacctcccg gggggcggaa cgatcgagct gctgacgccc tatccggtgg
     6181 gtggcgggag ccaccggctc tgggtcgcca ccgcctccgt cgggcgtccc ctggtcgagt
     6241 acctggagac ctggggccgc cccatccggt accgccatac cgacgggcct tatccgctcg
     6301 acgcctatca gacgatcttc gcccgggtct cgggctccgc cgagatgcca agcgcaggga
     6361 ggccattcac cgaccgcctg gtcaccgccc tcatgacggc aggtatcgcg gtggcgccgc
     6421 tgacgcttca cacgggtgtg tcttcactcg aagcgggtga ggacccatac ccggagtggt
     6481 tcgagatccc cgaatcgacc gcgtcgctcg tcaatcacac ccgagccaac ggagggcggg
     6541 tgatcgccgt cggcaccaca gtggtccgag ctctggagac gacggtggac gcgaaaggct
     6601 tgtcgcaccc agggcgaacc tggacggatc tcgtcatcgg ctccgatcac gccctcggcg
     6661 tcgtggacgg tctcatcacc ggatggcacg agccgcagtc gacccatctc gatttgctcg
     6721 aggcggtcgc tgggcggcgg cggctggctg agagctatcg caatgccctc gagcagggat
     6781 acatgtggca cgagttcggt gacagccacc tgatcgtgcc gggctgagaa gcaggacctt
     6841 cagccggtgg cgctattgct gccgatcctg gcgaacggct cggtggagaa gaccacttcg
     6901 atcggcactt cgaaatactc ggcgatcctc agcgccagga agaggcttgg gctgtactca
     6961 cctcgttcca ggtagccaat cgtctgataa tgaaccccca gcgcctcgct gagctggcga
     7021 cgggacactc cccgctcggc ccggagcatg gcgatccgat tgtgaaccgt gtcggtcatc
     7081 tgacccactg cacggcctgc tccttggctg cctccacctg ggagcccgac tggcgacgcg
     7141 ccatcctgcg caccaccacc ggggcgatga ggctgccggc cactgcccag gccgagagga
     7201 ccagcacggt ctggagagtt cgccacgagc cgccgatctc caggacagcc gcggcttcgg
     7261 gcaggaaagc cgaacgcatc cccagcccga cccagtacat ggggaacatc tgggacaccg
     7321 tctgcaccca cccccacaga tcctggatcg ggaagaagat ccctgagatg ccgaccagga
     7381 ccaggaccgg gaacatgccc cacgtgccga ccttctgcac gctgggggcg agggatccaa
     7441 tgatgatgcc gatgggaaga gtggcccaga gcccgaggag cgtcacccat gccacggtcg
     7501 cccaaccctc ggccccgttc tgcatgagcc cgtcgaacag cagcgcaccg gggatgagga
     7561 tgatcaggaa ggacgggacg atgctcagga tgttgaggat cacatgcccc gtgacgtggc
     7621 cgacgacgcc gtgcgggatg gccttggccc gaagcaaggt cccgtcctcg cgctccatgg
     7681 ccagcgcgta ggcggggccg attatgaggc tgaagaccaa caaggcaccc agcatgctgg
     7741 ggagcaccac cgtgggatag gacaggttcg tcccctccac ctcggtgttg cggttgaacc
     7801 agaggtaggc caggaccaat gcggccatga agagatagaa accctggtcc tgagggcttt
     7861 gcaggctcag cttgaactcg gtccatccac gggcgaaacc ggatttgatc gcattcgtcg
     7921 tcgggctcat cggtccacct cctcgaagac ggtgaccccc ggggtgacct ggccggactc
     7981 gtattgctgg accatggcga tataggtgtc ctccagagtg gcccggcgaa cctcgagg
//
LOCUS       DASTXO010000151         2543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1666948, whole genome shotgun sequence.
ACCESSION   DASTXO010000151 DASTXO010000000
VERSION     DASTXO010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1666948"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1338)
                     /locus_tag="VFU96_03260"
     CDS             complement(<1..1338)
                     /locus_tag="VFU96_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU5112308.1"
                     /translation="MTVVWQPTAERAAASNTAAFMRAHQIASLEELRRRSASDPEWFW
                     EKVVEFLGLPFDRPWRAIRDTSRGQAWATWFVGGGFNLSRACVDRWADEDPDRIALRS
                     EKETGETRELSFGGLKDVVSRLAGVLIALGVSRGDAVAVFLPMGEEAVVSLLAVSRIG
                     AIFIPVFSGYAAEAVATRLDDPRPKVMICADGFQRRGRPIEMKEVADQAIERAGGVEK
                     VVVVDYAGRGDTPMVEGRDVWWHELVTNAEPVAPSSTGSEDPVMIAYTSGTTGRPKGA
                     VHVQAGLTVKLAAEGAFHAEIGREDVVMWATDMGWIMGPWMVVAGLANGAALATYDGA
                     PDHPGPDRLWAVAANLGVTFLGISPTLIRALQPHGAEHARRHDLSQLRTFGSTGEPWN
                     PEPWRWLFHEVGEGRRPIINISGGTEIGAVIVGVNILQGLKPTSVGGPSFGIDA"
     gene            1460..>2543
                     /locus_tag="VFU96_03265"
     CDS             1460..>2543
                     /locus_tag="VFU96_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU5112309.1"
                     /translation="MNESHERLDNRVFRRRTLVVAGVLLLTALAVYLLFRLRNLLFMI
                     FVALFIAVAIEPAVHFLEKRGWRRGLATGLVFLVAFLLGVAFLALLVPLFINQVNDLI
                     DTLPAYIETVSTRLNEWFGLEISTESLQEEAAGLPNIIIGAGGTILGGVVSVTTGIFS
                     FLFFVTTVALFAFYMVADMPKLQRTVLSTMNPDRQRDALHIWEVALEKMGAYIYSRLI
                     LALLSGTLTAIFLTILGVPFAVSLGIWVGVLSQFIPVVGTYLAAILPAVVGLSSGGLG
                     TMLWVILYFVAYQQVENYLIAPRITQRTMEIHPAVSIGAIIIGSALFGPLGVILALPM
                     TGIIQALISETRSHHAVILDHGHDAIL"
BASE COUNT          401 a          885 c          774 g          483 t
ORIGIN      
        1 ggcgtcgatc ccgaaggaag ggccgcccac ggaggtcggc ttgagcccct gaaggatgtt
       61 gacaccgacg atgacggcgc cgatctcggt cccgcccgag atgttgatga tcggcctccg
      121 cccctcgccg acctcgtgga acagccagcg ccagggctct gggttccaag gctccccggt
      181 cgagccgaat gtgcggagtt gggagagatc atgcctgcgg gcgtgctcgg cgccgtgggg
      241 ctggagggcc cggatgagag tgggtgagat gccgaggaag gtgactccca ggttggccgc
      301 caccgcccag agccggtcgg ggccgggatg atctggggcg ccgtcgtagg ttgccagcgc
      361 cgccccgttg gccaggccgg cgaccaccat ccacggcccc atgatccatc ccatgtcggt
      421 ggcccacatc accacgtctt cccggccgat ctcggcatgg aaggcccctt cggcggcgag
      481 cttcaccgtg agaccggcct ggacgtggac ggcccccttt ggccgcccgg tggtgcccga
      541 agtgtatgcg atcatcacag ggtcctcgct gccggtggag ctcggcgcga cgggctcggc
      601 gttggtcacc agttcgtgcc accagacatc gcggccctcg accatggggg tgtcgcctcg
      661 cccggcgtag tcgacgacga cgaccttctc cacgccaccg gcgcgctcga tcgcttggtc
      721 ggccacctcc ttcatctcga tcggccggcc ccgccgctga aagccgtcgg cacagatcat
      781 caccttcggc ctcgggtcgt cgagccgtgt cgctaccgcc tcggccgcgt accccgagaa
      841 gaccggaatg aagatcgccc cgatacggct cacggcgaga agcgacacca cggcctcttc
      901 tcccatgggc aggaacacgg caacggcatc gcctcgggaa acgccgagtg cgatcaagac
      961 gccggcgaga cggctcacca cgtccttcaa cccgccaaac gacagctcac gggtctcacc
     1021 ggtctccttc tcacttcgca acgcgatgcg gtcagggtct tcgtctgccc acctgtcgac
     1081 acacgcccgg ctcaagttga agcccccgcc cacgaaccag gtggcccatg cttgccctct
     1141 cgacgtgtcc cggatggccc tccacgggcg gtcgaagggg agaccgagga actcgaccac
     1201 cttttcccag aaccactccg ggtccgatgc cgatctccga cgaagctcct ccaggctcgc
     1261 gatctgatga gcccgcatga atgcggcggt gttggaggcc gccgctctct ccgcagtggg
     1321 ctgccagacc actgtcatgt ctgggagacg acccccagac ccccggttgt cgaatggctc
     1381 catcgccctc ctcgtgggtg ttgcccgagg ctatatcgct gccgatacag tggcccggcc
     1441 ggtcctaacc tccccgtcga tgaacgagag ccacgagcgc ctcgacaatc gcgtgttccg
     1501 acggcggacc ctggtcgtag ccggagttct gctcctcacc gccctcgcgg tctatctgct
     1561 gttccgcctt cgaaacctgt tgttcatgat cttcgtcgcg ctcttcatcg ccgttgccat
     1621 cgagcctgcg gtccatttcc tggagaagcg tggctggagg agagggcttg caaccggatt
     1681 ggtctttctc gtggcgtttc tcctcggggt tgccttcctc gccctgctcg tgccgctgtt
     1741 catcaaccag gtcaacgacc tgatcgacac cttgcccgcg tacatcgaga cggtgtcgac
     1801 ccgattgaac gaatggttcg gcctcgagat ctcgaccgag tcgctccagg aggaagcggc
     1861 cgggctcccc aacatcatca tcggcgccgg cgggaccatc ctcggtggcg tcgtgagtgt
     1921 cacgaccggc atcttcagct ttctcttctt cgtcaccaca gtggccctgt ttgccttcta
     1981 catggtggcg gacatgccca agctgcagag gacggtcctc tccacgatga atcccgaccg
     2041 gcagcgggac gcgcttcaca tctgggaggt cgcgttggag aagatggggg cctacatcta
     2101 ttcgaggctg atcctcgcgc tgctgagcgg cactctgacc gcaatattcc tcacgatcct
     2161 cggtgtgccc ttcgcagtct cgctcgggat atgggtgggc gtgctcagcc aattcatccc
     2221 ggtggtcggc acctacctcg ctgccatcct tcccgcagtc gttggcctct cgtccggagg
     2281 tttgggcacg atgctgtggg tgatcctgta tttcgtggcc tatcagcagg tcgagaacta
     2341 cctgatcgcc ccccgcatca cccagcggac catggagatc caccccgccg tctcgatcgg
     2401 cgcgatcatc atcggctcgg ccctgttcgg gccactcggc gtgatcctcg cgctgccgat
     2461 gaccgggatc atccaggcgc tgatctcgga gacgaggagc catcatgccg tgatcctcga
     2521 tcatggccat gacgcgatcc tcg
//
LOCUS       DASTXO010000152         2001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_561811, whole genome shotgun sequence.
ACCESSION   DASTXO010000152 DASTXO010000000
VERSION     DASTXO010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561811"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VFU96_03270"
     CDS             <1..631
                     /locus_tag="VFU96_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEU5112310.1"
                     /translation="PFTEAVDHFEAQGSKDAVVMASGALKTIAVSLFKIANDIRFLGS
                     GPTSGIGEIRIPELQPGSSIMPGKVNPVMSEMMMQVAAQVVGNDTTVTWSGANGNFEL
                     NVMMPVMAHNLLESIDLLATASKTFAARCVDGIEVDAERVRELLERNAVVVTALNPHI
                     GYDNGARIVKEAAATGRSIRDLVLESGLMTPDELDAALDLKKMTEGGMA"
     gene            633..1853
                     /gene="xseA"
                     /locus_tag="VFU96_03275"
     CDS             633..1853
                     /gene="xseA"
                     /locus_tag="VFU96_03275"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU5112311.1"
                     /translation="MAEATLTVAGLLSTLDEAVATAVPGPVWVRGEVSGFQRTSRGAA
                     FFRLVDPDQPDNAVEVASSGRMMMTVTHALESAGVGGLRSGIEVRVRATVGVRHNRGH
                     IQLSLLEVDPSFTAGRLALDRDEVLRRLAGDGSLAANSRLDLPILPLRIGLVTSRGSA
                     AHADFLDQIRRPGYRFSVRTVQAAMQGERAVSNLVRALRRLAAEDIDVAAVVRGGGSK
                     LDLAAFDSEELGRAIAGMPVPVVTGIGHETDRTVADEAAAVSLKTPTAAAEWIVGRVS
                     DYAGRLDTARHTIRDAARSAWDRGAVRLDHSASQLAGTRGVLRRQMDQVDNLERAVAE
                     GSRTGLQRHREHLGVLAEMFAAIGVEPTLRRGFALVTRPDGSVVRSAGDLGAGDRVLV
                     KLADGSVPVVVESN"
BASE COUNT          350 a          593 c          693 g          365 t
ORIGIN      
        1 gccattcacc gaagccgtcg accatttcga ggctcaaggg tcgaaggacg ccgtggtgat
       61 ggcctcgggc gccctcaaga cgatcgccgt gtctctgttc aagatcgcca acgacatccg
      121 cttcctgggc tcagggccca cctcgggcat cggcgagatc aggatccccg aactgcaacc
      181 cgggtcatcg atcatgccag ggaaagtgaa cccggtcatg tcggagatga tgatgcaggt
      241 tgcggcgcag gtggtgggaa acgatacaac cgtcacctgg agcggggcga acgggaactt
      301 cgagctgaac gtgatgatgc cggtcatggc tcacaatctg ctcgagtcga tcgacctcct
      361 ggcaaccgcc tccaagacat ttgcggctcg atgtgtcgat gggatcgaag tcgatgccga
      421 gcgagtccgc gagctcctcg agcgcaatgc cgtggtggtg acagccctca atccacacat
      481 cggctacgac aatggggccc ggatcgtgaa ggaggccgcg gccacgggtc gttcgattcg
      541 agacctggtc ctcgagtccg ggctgatgac gcctgatgag ctcgatgccg ccctcgatct
      601 caagaagatg accgagggcg ggatggcctg aagtggccga agccacgctc accgtcgccg
      661 ggttgttgag caccctcgac gaagctgtag cgacagctgt tcccggtccg gtctgggtcc
      721 gtggcgaggt gtccgggttt caacggacca gccgcggtgc cgccttcttc cgtctggtcg
      781 atcccgacca gcccgacaac gcagtcgaag tggcttcgtc cgggcgaatg atgatgacgg
      841 tcacccatgc gttggaaagc gctggtgtcg gcgggttgag gagcggcatc gaggttcggg
      901 ttcgggcgac tgtcggtgtt cgccacaacc gggggcacat ccagctgagt ctcctcgagg
      961 tcgacccctc cttcactgcc ggtcgtctcg ccctcgatcg cgacgaggtg cttcgccgcc
     1021 tcgccggtga cggctcgctg gccgcaaaca gccggctcga tctaccgatc cttccgcttc
     1081 ggataggtct ggtgacaagt cgtggatcgg ctgcacacgc tgatttcctc gatcagatcc
     1141 ggcggcccgg ctatcgattc tcggtcagga ccgtgcaggc tgcgatgcaa ggagaaaggg
     1201 ccgtctcgaa cctggttcga gctctgcgtc ggctggcggc ggaagacatc gacgtggctg
     1261 ccgtggtcag gggaggaggg tcgaagctcg acctcgccgc gttcgattcc gaggagctcg
     1321 ggcgcgcgat agccgggatg ccggtgccgg tcgtcaccgg gatcggccac gagaccgacc
     1381 gaaccgtggc ggatgaggct gcggcggtct cgctgaaaac gccgacggcg gcggcggagt
     1441 ggatcgttgg tcgagtttcc gactacgcgg gcagattgga cactgcgaga cacacgattc
     1501 gggatgccgc ccgctcggca tgggaccggg gtgcggttcg cctcgaccat tcggcatccc
     1561 agctcgccgg aaccaggggt gtgttgcggc gtcagatgga ccaggtcgac aatctggagc
     1621 gggcagtggc agaagggtcc cggaccggtc tgcagcgaca ccgggagcac ctgggggtgt
     1681 tggctgagat gttcgccgcc atcggtgtcg agccgacttt gagacgagga tttgccctcg
     1741 tcaccaggcc cgatggatcg gtggtgcgca gcgccggtga tttgggcgcc ggtgatcgtg
     1801 tcctggtgaa actggccgac ggctcggtgc cggtggtggt ggaatcaaat tgaccgattc
     1861 gcaacagacc tatcgggagg cgctggagga gctcagggtg atacacgccc ggctacggga
     1921 agatgaggtg gacatcgaca ccctgcttgc cgacgtgcaa cgggcatcgg atttactcgc
     1981 cttctgccgc gagcgtctca a
//
LOCUS       DASTXO010000153         2417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1630360, whole genome shotgun sequence.
ACCESSION   DASTXO010000153 DASTXO010000000
VERSION     DASTXO010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630360"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..1440
                     /locus_tag="VFU96_03280"
     CDS             199..1440
                     /locus_tag="VFU96_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU5112312.1"
                     /translation="MNRTQITAGRDAHHRPKGVSADELRRHNLAVVVESLHLHGPVSR
                     SELTATTGLNRSTIADLIGELTNLGLVEEGPAVTPSGPGRPSPIVSTRPQGAIVLSIE
                     LSVDSVAVATVGLGGHVYEQVRVPRPRGESAAETVGVVAGLSGELLAGLPADSTLTGI
                     GVGVAGVTRRSDGFVQLAPNLGWRNVALGEMLASALGLSDPILVANEADLGALAEFRR
                     GVGVGVPHLIYISGEAGIGAGIIHDGKPMLGAAGYAGEAGHTLINPDGQACRCGAIGC
                     WETEAGEAALARLAGIPSSVTGLGLVGSIVARADAGDQKTLDALAEIGRWLGLGIGNL
                     INVFNPQLVVLGGFYHPLFPFLEKAVFEGARLGALDAPGDIADIAAGGLGPDAPLIGA
                     AELAISAVIADPAGHTRARSG"
     gene            complement(1447..2415)
                     /locus_tag="VFU96_03285"
     CDS             complement(1447..2415)
                     /locus_tag="VFU96_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5112313.1"
                     /translation="MGCADIAVKKVIPAMNGSELSHVTAIASRSSDRARATADTLGIP
                     RSYGSYEELLADPEIEAVYIPLPNHLHPEWAIASAEAGKHVLCEKPLATSSAVAKQMI
                     DAADRSGVKLMEAFMYRLHPLWVEVRRLLAEGAVGELRAIHSFFSYHNIDPEDIRNRV
                     DAGGGSLHDIGCYPVNVARMLFDSEPSDVIAAIRRDPDFGTDVLTSVVLDFDGRHAAF
                     TCSTQLEDDQRVHVYGTAGRLVIEIPFNIPPDRPTRVLLVSGGDPPVDPNVVVHQVPA
                     TDQYRVQADAFSRAIRDDTPVPTPPQDAIANIEVMERIFADAASRA"
BASE COUNT          401 a          775 c          815 g          426 t
ORIGIN      
        1 attaggcgtc ttttcatctg attttccttc ctcctccggt tgccacccca aaggggctgg
       61 caattaaccg tacgagtcga gcgcgaatgg gccatgacga ccccacgccg accgatgcga
      121 gcataaccgt catatgtttg accccgcaac aaattgcggc atagggttct ggctcacaat
      181 cgacctcccg ggagacactt gaaccgcacc cagataacgg ccgggaggga tgcccatcac
      241 cggcccaagg gcgtatccgc cgacgagctg cgccgtcaca atctggcggt tgttgtggag
      301 agcctccatc tccacggccc ggtctctcgc tctgagctga ctgccaccac cgggctcaac
      361 cgatccacca tcgccgacct catcggcgag ttgaccaacc tcggcctcgt cgaggagggc
      421 ccggcggtca cgccatcggg cccgggtcgt ccctccccaa tcgtgagcac ccggccgcag
      481 ggcgccatcg ttctctccat cgagttgtcg gtcgactcgg tcgcagtggc cacggttggc
      541 ctcggcggtc acgtctacga gcaagtgcgg gtgccacggc cgcgcggaga atcggcggcc
      601 gagacggtcg gagtcgtcgc cggcctgtct ggggaactgc tggcggggct ccccgccgat
      661 tcgacgctga ccggaatcgg cgtcggtgtc gccggggtca cccggagatc agacgggttc
      721 gtacaactcg cccccaacct cggctggcgg aacgtggctc tcggcgagat gctcgcttcc
      781 gcgcttgggc tgagcgaccc gatcctcgtg gccaacgaag ccgacctcgg cgcgctggcg
      841 gagttccgac gcggagtcgg tgtcggagtg ccccatctca tctacatctc tggtgaggcc
      901 gggatcgggg cgggaataat ccacgacggg aagccaatgt tgggagctgc cggctacgcg
      961 ggcgaggccg ggcacaccct gatcaacccc gacggccaag cctgtcggtg cggggccatc
     1021 ggctgctggg agaccgaggc cggcgaggct gcgctggccc gtcttgccgg gatcccgtcg
     1081 agtgtcaccg ggctgggact cgtgggctcc atcgtggccc gggcggatgc cggcgaccaa
     1141 aagactctcg atgccctcgc cgagataggg cgatggctcg gattggggat cggcaacctg
     1201 atcaacgtct tcaacccgca gttggtggtg ctcggcgggt tctatcaccc gctcttcccg
     1261 ttcctggaga aggccgtctt cgaaggggcc cggcttggcg cgctcgatgc cccgggtgac
     1321 atcgccgaca tcgcagctgg cgggctcggg cccgatgccc cgctgatcgg tgcggccgag
     1381 ctggccatct cggcggtcat cgccgacccg gccggacaca ctcgcgcccg gtccgggtga
     1441 cgcggctcag gcgcggctgg cggcgtcggc gaagattcgc tccatgacct cgatgttggc
     1501 gatggcgtcc tgtggtggtg ttggcaccgg cgtgtcgtca cgtatggcgc gggagaaggc
     1561 gtcggcctgc acccggtatt ggtcggtggc tgggacctga tgaaccacca cattggggtc
     1621 gaccggtggg tctccaccgg agaccaacag gacgcgggtg ggacgatccg gtgggatgtt
     1681 gaacgggatc tcgatgacga gacgaccggc agtcccatag acgtggacac gctgatcgtc
     1741 ctccaattga gtcgaacagg tgaacgccgc gtgccgccca tcaaagtcga gcaccacaga
     1801 ggtcaggacg tcggtgccga aatcgggatc ccgtcggatg gcggcgatca cgtcggatgg
     1861 ctcggagtcg aagagcatcc gggcgacgtt gaccgggtag caaccgatgt cgtggaggga
     1921 tcccccgccg gcgtcgactc ggttccgaat gtcttcgggg tcgatgttgt gataggagaa
     1981 gaacgaatgg atcgcgcgca actcgccaac ggctccttcg gcgagcagcc tccggacctc
     2041 gacccagagc ggatgcaggc gatacatgaa cgcctccata agcttcaccc cggatcggtc
     2101 agccgcgtcg atcatctgct tggctaccgc gctgctcgtt gcgagcggtt tctcacagag
     2161 cacgtgcttg ccggcctcag cggatgcaat ggcccattcc ggatggagat gattgggcaa
     2221 cggtatgtag acggcctcta tctcagggtc cgcgagcagt tcctcgtagc tcccgtagga
     2281 ccggggaatg ccgagggtgt ctgccgttgc ccgggcccga tccgaggatc gcgaggcgat
     2341 ggcggtgaca tggctgagct ccgacccgtt catggccggg atcaccttct tgaccgcgat
     2401 atcggcacag ccgatca
//
LOCUS       DASTXO010000154         2050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_120337, whole genome shotgun sequence.
ACCESSION   DASTXO010000154 DASTXO010000000
VERSION     DASTXO010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120337"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VFU96_03290"
     CDS             <1..350
                     /locus_tag="VFU96_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEU5112314.1"
                     /translation="EEGGSLTILGTALVETGSKMDEVIFEEFKGTGNMELRLDRKLAD
                     KRIYPAIDIEASGTRKEELLFDEKELAQVWKLRRVLLALEPGAALELLIDRLKTTKSN
                     AEFLAEVAKSSGS"
     gene            364..630
                     /gene="rpmE"
                     /locus_tag="VFU96_03295"
     CDS             364..630
                     /gene="rpmE"
                     /locus_tag="VFU96_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEU5112315.1"
                     /translation="MKTDIHPTYAETHVTCSCGNTFTTRSTKPGDMHIELCNECHPFF
                     TGKQKLVDSGGRVERFQKRYGKTAFTKAGDPVEADAEAETAEAE"
     gene            674..1672
                     /locus_tag="VFU96_03300"
     CDS             674..1672
                     /locus_tag="VFU96_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEU5112316.1"
                     /translation="MTSERPNYEGPTGPIDPAKTVGGQAVIEGVMMRAPGAWSVAVRK
                     PDDTIVARRHVLGRLADRNRAARIPFVRGIVVLWESLSLGFRALSWSAEMAAGEEEEP
                     LSKTQIGWTMALAFALFAGIFIVLPALAAGALSGESGILFNVTEGVIRLALFIGYIWA
                     IGRSAEIGRVFEYHGAEHMSIHAFEAGEPLSVESVRRYPPEHPRCGTSFLLIVVLGSI
                     ILFSFFGRPGWAFLVTSRLLGIPIIAGLAYELLRWSGTRSSGWLARALAAPGIWLQKL
                     TTRVPDESQIEVAISSLVVALDNEMVDDVIARGGLPGTALEVRRAAFGVAPPAAGA"
     gene            1672..>2050
                     /locus_tag="VFU96_03305"
     CDS             1672..>2050
                     /locus_tag="VFU96_03305"
                     /inference="COORDINATES: protein motif:HMM:NF015427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HEU5112317.1"
                     /translation="MDDTLERRLSEIESAFEETEAALSDPETLANPGRLAELGKRHAD
                     LREVVSDIRQIRRLREDLEEARAMAEDPDMADLARELVDEIDGIEARVRLSLIPTDPA
                     DAKDVILEIRSAAGGDEAAIWAGD"
BASE COUNT          369 a          654 c          676 g          351 t
ORIGIN      
        1 tcgaggaggg gggctcgctc accatcttgg ggaccgcgct cgtcgagacc gggtcaaaga
       61 tggacgaggt catcttcgag gagttcaagg gaaccggcaa catggagctc cgtctggacc
      121 gcaagctcgc cgacaagcgg atctacccgg ccatcgacat cgaggcgtcg ggaacaagga
      181 aggaagagct gctgttcgac gagaaggagc tcgcccaggt gtggaaactc cgcagagtgc
      241 ttctggctct ggagccggga gccgctctgg agctcctcat cgaccgattg aagaccacga
      301 agtccaacgc cgagtttctc gccgaagtgg cgaagagcag cggcagctag ataggagcca
      361 gcggtgaaga ccgacattca tccgacatac gccgagaccc acgtcacgtg ctcgtgtggc
      421 aacaccttca ccacccggag cacgaagccg ggcgacatgc acatcgagtt gtgcaatgag
      481 tgccacccgt tcttcaccgg caagcagaaa ctggtcgaca gtggtggtcg cgtggagcga
      541 ttccagaagc gatatggcaa gaccgccttc accaaagccg gcgatccagt cgaagccgac
      601 gcagaggcgg aaaccgccga ggcggagtga gtcgacggct tccgacgaga cgccgggagc
      661 cacagtcccg catttgacca gcgagcgccc caactacgaa gggccgaccg gccccattga
      721 cccggccaag acagtcgggg gccaggcagt gatcgaaggc gtgatgatgc gcgcccccgg
      781 tgcctggtcc gtggctgtcc gcaagcccga cgacaccatt gtggcccgtc gtcatgtcct
      841 cggccgtctg gccgaccgca atcgcgccgc ccgaatcccg ttcgtgcgcg gcatcgtcgt
      901 gctctgggag tcgctgagcc ttggatttcg cgccttgtcg tggtcggccg aaatggctgc
      961 cggcgaggag gaagagccgc tgagcaagac ccagatcggt tggacaatgg cactggcctt
     1021 cgccctgttc gccggcatct tcatcgtcct cccggctctc gccgcgggtg cgttgtcggg
     1081 cgaatcgggg atcttgttca acgtcaccga gggagttatc cgcctcgccc tgttcatcgg
     1141 ctacatctgg gcgatcggcc gctctgccga aatcgggcga gtattcgagt accacggggc
     1201 cgagcacatg tcgatccacg ccttcgaggc gggggagccg ctcagtgtcg aatcggtgcg
     1261 ccgctatccc ccggagcatc cccggtgcgg cacgagcttc ttgctcatcg tcgtgctcgg
     1321 ctcgatcatc ctcttttcct tctttggtag gccgggatgg gccttcctcg tcacgtcgcg
     1381 cttgctcggc atcccgatca tcgccggcct cgcctacgag ttgttgcggt ggtccggcac
     1441 ccggagctcg ggctggctgg ctcgggccct cgccgctccc gggatctggc tgcaaaaact
     1501 gacgacgagg gtgccagacg aatcgcagat cgaggttgcc atctcgagtc tcgtggtcgc
     1561 cctcgacaac gagatggtcg acgacgtcat cgcccgcggc gggctgcctg ggacggcgct
     1621 cgaggtacga cgcgccgctt tcggtgtggc gcctccggcc gccggcgcct gatggacgac
     1681 acgctcgagc ggagactcag cgaaatcgag tctgccttcg aagagaccga ggcggcactg
     1741 tccgaccccg agacgctggc caatcctggg aggcttgccg agttggggaa gcgccatgcc
     1801 gaccttcgcg aggtggtctc cgacatccgc cagatccgtc gactgcgcga ggatctcgag
     1861 gaggcccgtg ccatggctga ggaccccgac atggccgatc tggctcgaga gctcgtcgac
     1921 gagatcgacg ggatcgaagc gcgggtcagg ctttccctga ttccaactga cccggccgat
     1981 gccaaggacg tgatactcga gatccgatct gccgccggcg gcgatgaggc agccatctgg
     2041 gcgggagatc
//
LOCUS       DASTXO010000155         2383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_857164, whole genome shotgun sequence.
ACCESSION   DASTXO010000155 DASTXO010000000
VERSION     DASTXO010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857164"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1561)
                     /locus_tag="VFU96_03310"
     CDS             complement(<1..1561)
                     /locus_tag="VFU96_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012836058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEU5112318.1"
                     /translation="MDLNRLTEKSQQALSEAQSMAVRLGHTEVDGEHLLLALVTQSEG
                     LVPRLISQAGGEPARLAAEMEAELAKRPSVSGPGVSPGEIRVTQRLARLLDSAEREAT
                     RLKDEYVSVEHLVIAFLGEADTAAKRALAEQGITRDRFLEALTAIRGNQRVTSATPES
                     AYEALEKYGRDLVGDARSGKLDPVIGRDTEIRRVIQILSRKTKNNPVLIGDPGVGKTA
                     IVEGLAQRIMRGDVPEGLRDKTVFALDMGALIAGAKYRGEFEERLKAVLGEVKAAEGR
                     ILLFVDELHTVVGAGAAEGAMDAGTMLKPMLARGELHMIGATTLAEYRKHIEKDAALE
                     RRFQTVLVDEPTVEDAISILRGLRERFEVFHGVKIQDAALVAASVLSHRYITDRFLPD
                     KAIDLVDEACAMLRTEIDSMPAELDELTRRVTRLEIEEAALSKEKDASSKKRLEELRR
                     ELADLKTDAAAMRSRWESERQILREVQELRAQMERVHRETEEAERNYDLDRAAQLRHG
                     ELPELERKVKEA"
     gene            1648..2190
                     /locus_tag="VFU96_03315"
     CDS             1648..2190
                     /locus_tag="VFU96_03315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEU5112319.1"
                     /translation="MQESRRHVPSTEIGALDVEPFESFYRREYSRMVAIARALLRTGA
                     SAEDLAQESFMAAHRKWDQVSGYDDPGAWVRRVLINRATSLHRKAGAELRALTRLGGH
                     GGEDTLPELTPRATEVWDEVRKLPRRQAQATVLHYVDQLSIAEIGEVMGVSPGAVKAH
                     LHKARAHLSRRLETWGEETI"
     gene            2187..>2383
                     /locus_tag="VFU96_03320"
     CDS             2187..>2383
                     /locus_tag="VFU96_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112320.1"
                     /translation="MKHLDEFEQRMEQAAQEVRQSAKHAVPPALDSDASRAIPRGWLI
                     FAVAFGVVILTLGVIPMISSP"
BASE COUNT          373 a          790 c          774 g          446 t
ORIGIN      
        1 cggcttcctt caccttgcgc tccagctcgg ggagctcgcc atgccgcagc tgggcggcgc
       61 ggtccaggtc gtaattcctc tccgcctcct cggtctcccg gtgcacgcgt tccatctggg
      121 ctcgaagctc ctgcacttcc cgcaagatct gccgttcgga ctcccaccgc gacctcatcg
      181 cggcagcgtc ggtcttcaag tcggcgagct ccctcctcag ctcctcgagg cgcttcttgc
      241 tggaggcgtc cttctccttg ctcaaggcgg cctcttcgat ctcgagccgg gtgacgcgcc
      301 tggtcagctc gtcgagctcg gccggcatgg agtcgatctc cgtgcgcaac atggcacagg
      361 cctcgtcgac gagatcgatc gccttgtcgg ggaggaatcg gtcggtgatg tagcggtggc
      421 tgagcacgga ggctgcgacc agggctgcgt cctggatctt gaccccatgg aagacctcga
      481 agcgctcacg caggccgcgg agaatcgaaa ttgcgtcctc cacggtcggc tcatcgacca
      541 gcaccgtctg gaatcgccgc tccagggcgg cgtccttctc gatgtgtttc cggtactcgg
      601 ccaatgtcgt ggcaccgatc atgtgcagct cgccgcgggc gagcatcggc ttgagcatgg
      661 tcccggcgtc catcgccccc tctgctgcac cagcaccgac gacggtgtgc agctcgtcga
      721 cgaagaggag gatccggcct tcagccgcct tcacctcgcc gagaactgcc ttgagacgct
      781 cctcgaactc accgcggtac ttggccccgg cgatcaatgc ccccatgtcg agggcgaaca
      841 ctgtcttgtc ccgaagccct tcggggacat cgccgcgcat gatgcgctgg gccagcccct
      901 cgacgatggc cgtcttgccc accccggggt cgccgatcag cacagggttg ttcttggtct
      961 ttctggacag gatctggatc acccgacgga tttcggtgtc gcgcccgatc accgggtcga
     1021 gcttcccgct cctggcgtcg ccgaccaggt cacggccgta cttctcgagt gcctcgtagg
     1081 ccgattcggg cgtggccgag gtcaccctct ggttgcctcg gattgcggtc agcgcctcca
     1141 gaaatcggtc tcgcgtgatg ccctgttcgg cgagggcgcg cttggccgcg gtgtctgcct
     1201 ccccgaggaa ggcaatgacg aggtgctcca ccgacacgta ttcgtccttg agacgtgtcg
     1261 cctccctctc tgccgagtcg aggagcctgg ccagacgttg ggtcacccgg atctcaccgg
     1321 gggagacccc ggggccgctg acactggggc gcttggcgag ctccgcctcc atttcggcgg
     1381 cgagtcgggc gggctcgccg ccggcctggg agatgagccg gggtaccaaa ccctcggatt
     1441 gggtcacgag ggcaaggagg agatgctccc cgtcgacctc ggtgtgtccg agacgaaccg
     1501 ccatcgactg cgcttctgag agcgcctgct gcgatttctc ggtgagtcgg ttcagatcca
     1561 tgctcgatcc gaatcctatt tcataccctt gccgcgactc cgcgtaaacc agttagggcg
     1621 ctggtgcgtg ttaggtgggt gagcacgatg caggaatcga ggagacacgt gccttcgaca
     1681 gagattggag cgctcgacgt cgagcccttc gagagcttct accggcgtga gtactcccgg
     1741 atggtggcga tcgcccgcgc ccttctccga accggtgctt cggcagaaga cctcgcccag
     1801 gaatcgttca tggcggccca ccgaaagtgg gaccaggttt cgggctacga cgaccccggc
     1861 gcctgggtgc gccgggtgct gatcaaccgg gccacatctc tccaccgcaa ggccggagcg
     1921 gaactccggg ccctgacccg gttgggtggc catggcggcg aagacactct ccccgagctg
     1981 acgccccggg ccaccgaggt gtgggatgag gtgaggaagc tcccgaggcg acaagctcag
     2041 gccaccgtcc tccactacgt ggaccaactg tcgatcgccg agatcgggga ggtcatgggg
     2101 gtgagcccgg gggcggtgaa ggcacatctg cacaaggcgc gggcacatct cagccggcgt
     2161 ctggagactt ggggcgagga gacgatatga agcatctcga cgaattcgag caacggatgg
     2221 aacaggctgc acaggaagtg cgccagtccg ccaagcatgc cgtgccgccc gccctcgact
     2281 cggatgcaag ccgagccatt ccccgggggt ggctgatctt cgcggttgcc ttcggtgtcg
     2341 tgatcctgac cctgggtgtg atcccgatga tctcgagccc gga
//
LOCUS       DASTXO010000156         3600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_930782, whole genome shotgun sequence.
ACCESSION   DASTXO010000156 DASTXO010000000
VERSION     DASTXO010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930782"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1901
                     /locus_tag="VFU96_03325"
     CDS             <1..1901
                     /locus_tag="VFU96_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEU5112321.1"
                     /translation="VPGDGAAWTTPGADLRLSAGLPGIYDALPHERDRKDLGLFLGLL
                     DSPAGGLVLFGRPSKFTDLAPDQRAAALLAMERNRVEIVRKGVRALKTLAAFLWVTTD
                     DPSRPPPAWAAMRYPGPDGPPSVAIKPIQTEAVHRDTTIDCDVVVVGSGAGGGIAAAL
                     LAAAGLDVTLLERGGYNDESDFTHLESDAYRSMYLDGGLGSTSDGGVLMLAGSTLGGG
                     TVVNYTTSLATPDQVREEWDRVAGFPEVFSGDDYLSSLASVQERMDVNRDNGLPSVRD
                     QVLEKGIRNLGWHLDEMPRNASGCTEEACGYCTMGCRVGAKRSTLATYLQDAAEGSVR
                     FIVDADARLVVTERGRATGVVARVRDHDLTVRASTVVLAAGALNTPALLLRSGLGGPA
                     AGRYLRLHPVTAAWGRFEERIDPWTGTLQTRYSDEFANLDGHGYGFRFETAPVHPLFP
                     AAYVGWEDGPSFKRDVLGLGHLGVAGILLRDRDHGRVVVRRDGSPLWKYRISSFDQAH
                     VREGVRRAAELLAAAGAVEVFSSTIRPVRWVPGQGTIDGLMAGVDAVGYGPNQTSYLS
                     FHQMGSARMGSDPSTSVVDAENQAHDTAGLYVMDGSCFPTASGVNPMISIAAIAHRGA
                     TRLAERIA"
     gene            complement(1904..2197)
                     /locus_tag="VFU96_03330"
     CDS             complement(1904..2197)
                     /locus_tag="VFU96_03330"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU5112322.1"
                     /translation="MEAGVGDVTVAGALITGRALRADGSPVSEARVFFAAAPVPLPDV
                     ATLTDDDGRYSLNAPAPGRYELVCQADDFDPATVAIDVPAHSGSIELDFELGG"
     gene            complement(2173..3135)
                     /locus_tag="VFU96_03335"
     CDS             complement(2173..3135)
                     /locus_tag="VFU96_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HEU5112323.1"
                     /translation="MPEGIEVQGKDTPVSSFDVGEESVSDEISTGEDDQVEKTPRSGF
                     EKIEGYRPPTPPTHSEFSPESVGDVLSVPDETTQLPDIGEASFGPPDARPETVHGADD
                     RFQITNTAAYPWRAHASLRITAADNSLWIGTGWFVGPRLLITAGHVVYIKNSGIADRD
                     GWVQKIVVMPGRNGGTLPFGSATTSSFWTVAGWATDGDGEYDYGAIVLSESLGDRTGW
                     FGFGNWPSLDGVHANISGYPGDKPSGTQWYAARTVDSASTRKVFYDIDTAGGQSGSAV
                     YRFANGSRYGIAIHAYGGATVNSGTRINQPVFDNIKTWKQASET"
     gene            complement(3159..3458)
                     /locus_tag="VFU96_03340"
     CDS             complement(3159..3458)
                     /locus_tag="VFU96_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112324.1"
                     /translation="MASRSHVERAIEQYQEELARYPNVTGIGVVPVADDSDGPTDLAV
                     AVYVTKKVPRDQLKAGERIPRSLEITSRDGSRNVPTRIIATGEFALEDQFDVEPA"
BASE COUNT          565 a         1188 c         1156 g          691 t
ORIGIN      
        1 ccgttcctgg cgacggtgct gcctggacca cccccggcgc ggatctccgg ctatcggcgg
       61 gactccccgg catctacgac gcacttcctc acgagcgcga tcggaaggat ctcgggctct
      121 tcctcggtct cctcgactcc cccgccggtg gtctggtgct cttcggccgt ccgtcgaagt
      181 tcaccgacct ggcccccgac cagcgggctg ccgccctgct cgccatggaa cgcaaccggg
      241 tggagatcgt ccgcaagggg gtccgagcct tgaagaccct ggctgctttc ctatgggtca
      301 ccaccgacga tccgtcccgt ccgcctccgg cctgggccgc catgcgatac ccgggcccgg
      361 atggccctcc ctcggtggcg atcaagccga tacaaacaga ggcagtgcat cgggacacga
      421 ccatcgactg tgatgtcgtg gtggtcggat cgggagccgg tggagggatc gcggcggcat
      481 tgctcgctgc cgccggactc gacgtgacgc tgctcgagcg tggcggatac aacgatgagt
      541 ccgacttcac ccatctggag tcggatgcct atcgctccat gtatctcgac ggcggtctcg
      601 ggtccacctc agacggtggc gtcctcatgc tcgccgggtc cactttgggt ggcggcaccg
      661 tggtcaacta cacgacgtcg ctcgccacgc ccgatcaggt gcgcgaggaa tgggaccgcg
      721 tggctggatt tcctgaggtt ttcagcggcg atgactacct gagctccctc gcctccgtgc
      781 aggagaggat ggacgtcaac cgggacaatg ggctgccctc tgtacgtgat caagttctgg
      841 agaaaggcat ccgcaacctg ggctggcacc tcgatgagat gccccggaat gcgtccggtt
      901 gcaccgaaga ggcctgtggc tactgcacca tgggctgcag ggtcggggcc aagcgatcga
      961 ccctcgccac ctatctccag gatgcagccg aggggagtgt ccgattcatc gtcgatgcag
     1021 atgcccggct cgtcgtcacc gaaaggggtc gtgccacggg tgtggtcgct cgggtcaggg
     1081 accacgatct caccgtgcga gccagcacgg tggtgctcgc cgccggcgcg ctcaacactc
     1141 cggcattgct cctccgaagc ggtctgggcg gcccggcggc cggacgatat ctgaggctcc
     1201 atccagtgac cgccgcttgg ggacgattcg aagagcgcat tgacccatgg accgggacgc
     1261 tgcagaccag gtacagcgac gagttcgcca acttggatgg tcacggctac gggttccggt
     1321 tcgagactgc tccggtccac ccgctgttcc cggccgcata cgttggctgg gaggatggtc
     1381 catcgttcaa gcgcgatgtc ctcgggttgg gccatcttgg ggtggccggg atcctgttgc
     1441 gggatcgtga ccacggccgg gttgtcgttc ggcgtgatgg cagcccgctc tggaagtacc
     1501 ggatctccag cttcgaccag gcacacgttc gagaaggagt gcgtcgggct gccgaattgc
     1561 tcgccgcggc gggagccgtc gaggtgttct cgtcgaccat ccggcccgtg cggtgggttc
     1621 caggccaagg caccatcgac ggtctgatgg ccggtgtcga cgcagtcggg tacgggccaa
     1681 atcagacctc atatctttcg ttccatcaga tgggaagcgc ccgcatgggg tcggacccgt
     1741 cgacctctgt ggtcgacgcc gagaaccagg cccatgacac agcagggctc tatgtgatgg
     1801 atggttcctg cttcccgacg gcgtcggggg tgaacccgat gatctccatc gccgcaatcg
     1861 ctcatcgcgg cgcgacccgg ttggcggagc ggatcgcctg agctcagccg ccgagctcga
     1921 agtcgagctc gatgctccca gagtgtgccg gcacgtcgat ggctaccgtc gccgggtcga
     1981 agtcatctgc ctgacagacc aattcgtacc tgccgggcgc cggtgcgttc aaggagtatc
     2041 ggccgtcgtc atcggtgagt gtggcgacat cgggcagcgg cactggcgca gccgcaaaga
     2101 acacccgcgc ctcgctgacc gggctgccat cggcgcgcaa ggcccgtccg gtgatcaggg
     2161 cccctgcgac cgtcacgtct ccgacgcctg cttccacgtc ttgatgttgt cgaagacagg
     2221 ctggttgatc cgggtccccg aattgaccgt cgcgccaccg taggcatgga tggctatgcc
     2281 gtatctggat ccgttggcga atcggtagac ggcggatccg ctctggcccc cggcggtgtc
     2341 gatgtcgtag aacaccttcc gggtgctcgc cgagtcgact gtcctggccg cataccactg
     2401 agtgccggac ggcttgtcac cgggataccc ggaaatgttg gcgtgcacgc cgtcgagact
     2461 cggccagttt ccgaacccga accagccggt cctgtcgccg agcgactcgc tcaggacgat
     2521 ggcgccgtag tcgtactcgc cgtctccatc cgtggcccac ccggcgactg tccagaagct
     2581 ggaagtcgtc gccgacccga agggcaaggt ccccccgttt cgacctggca tcacgacgat
     2641 cttctgcacc cacccgtcgc ggtctgcgat gccgctgttc ttgatataga ccacgtggcc
     2701 cgcggtgatc agcagccggg gcccgacgaa ccatcctgtc ccgatccaca acgagttgtc
     2761 cgcggcggtg atgcgaagag acgcatgggc ccgccatggg tatgcggccg tgttggtgat
     2821 ctggaatcga tcgtccgcgc cgtgcactgt ctccggcctg gcatctggcg gcccaaacga
     2881 tgcctcgccg atgtcgggca actgtgtggt ctcgtccggg acggacaaca cgtctcctac
     2941 cgactctggc gagaactcgc tgtgcgtcgg gggtgtcggg ggccggtagc cctctatctt
     3001 ctcgaacccg ctcctcgggg tcttctcgac ttgatcgtct tcaccagtcg agatctcgtc
     3061 tgagaccgac tcctcgccga cgtcgaagct cgacaccgga gtgtccttgc cttgcacctc
     3121 aattccctcg ggcatgacag ccctccttcc tcgatggcct atgccggttc gacgtcgaat
     3181 tgatcctcga gcgcgaactc ccctgtggcg atgattcggg tgggcacatt ccgcgacccg
     3241 tcccggctcg tgatctccag cgagcgcggt attcgctccc cggccttcaa ctggtcgcgc
     3301 gggaccttct tggtgacata caccgcgacc gcgaggtctg ttggaccgtc ggaatcatcg
     3361 gccaccggga cgactccgat cccggtcaca tttgggtacc gcgccagctc ctcctgatat
     3421 tgctcgatgg cgcgctcgac gtgactccgg ctggccatca caccacctcg atgtccagca
     3481 actcgaggac gtgggtgatg cgattgaaga cgctgcccga cgttgcgccg gcgaaatgga
     3541 ggccgacgat cttgtcgttg ctgttgagga tcaccgagcc gctgtcgcct gccgcgctgt
//
LOCUS       DASTXO010000157         2124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1188852, whole genome shotgun sequence.
ACCESSION   DASTXO010000157 DASTXO010000000
VERSION     DASTXO010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188852"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VFU96_03345"
     CDS             complement(<1..561)
                     /locus_tag="VFU96_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112325.1"
                     /translation="MADTASQSSSGVRTAEIIAAISMATDLAVGFPLEHGLQSCVIAT
                     RLCDRLDVDRDTASQAYFLSLLFYVGCNAPAEVGWDVFGDDDSLTTYGTPFRFGTRAE
                     MAGGMMRAVAPPTPAPHLRVWRLPRHFPELAIGFAGVVDATCEVARMLIDELGLGPPV
                     SNLVAFESTRWDGKGLPGGVAGEEIPL"
     gene            complement(635..>2124)
                     /locus_tag="VFU96_03350"
     CDS             complement(635..>2124)
                     /locus_tag="VFU96_03350"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5112326.1"
                     /translation="GGSPALGLVAFLQLAPAALFAPVGATLGDRYRREVMLTVAYAVF
                     AASSASVAVALYADWSVGWIYLLAAVNATVLTLVRPFHESLLPALARSTEQLTAGYVA
                     GGTIENVGWIAGPLLAGLIAAAWGIGAVFVITTVLLLLGTILVWSVRTSTAADQGTDQ
                     SAIQMIVDGFDAFREPRPRVILLMLTSVVFLAGVLEVATIVLAFEVFGSEDAGAAWLN
                     SAIGIGAATGSVLAVVLIGKRHLAPSMRRGLLLTAVSVSLIPVFGSQAVALVALAVMG
                     AGWTFVDVSGRIMLQRVIPLGGLSRAFGVLEGTSMAAEALGSVTGAVLIGWLGVGWAL
                     VVASLLIIMVLLVYRGALQEADVGVIVPIEHLHLLRSAPMFAALGPAELERLARTIAP
                     ESVVQGAAVCVQGEPGEHFYFVERGTARVLRDGTDVATLGIGDFFGEIALLRDIPRTA
                     TVEAVSDLDLLVLERGPFLDAIKSSACQIEAARAVTQTRMDELGL"
BASE COUNT          430 a          708 c          695 g          291 t
ORIGIN      
        1 caaagggatc tcttctccgg cgactccacc cggcaagccc ttgccatccc aacgggtcga
       61 ctcgaaagcc acgaggttgg agacgggggg gcccaggccc aactcgtcga tcagcatccg
      121 ggccacctcg caggtggcat cgaccacgcc cgcgaacccg atcgccagct ctgggaagtg
      181 gcgagggaga cgccagacac gaagatgagg tgccggggtt ggcggtgcca ccgctcgcat
      241 cattcccccg gccatttccg cccgggtgcc aaagcggaag ggagtgccat aggtcgtgag
      301 tgagtcgtcg tcgccaaata cgtcccagcc cacctcggcc ggggcattgc agccaacgta
      361 gaagagaagg ctcaggaagt acgcctgcga cgccgtgtcc cggtcgacgt cgagacgatc
      421 gcacaggcgg gtcgcgatca cacagctttg tagaccgtgc tccaagggga atcccacggc
      481 taggtcggtt gccatcgaga tcgcggcgat gatctccgcg gttcgaaccc cgctcgaact
      541 ctggctggcg gtatccgcca tcccgcgacc ctacgtggga gctgggatca caggggtctc
      601 tgctctgact ggcctgacct catgaccgtt cgactcacaa ccccaattcg tccatgcggg
      661 tctgggtgac cgcccgcgca gcttcgatct gacaggccga cgacttgatg gcgtcgagga
      721 acggccccct ctcgaggacg agcaggtcca ggtcgctcac tgcttcgacg gtggcggtcc
      781 ggggaatgtc cctcaacaag gcgatctccc cgaagaagtc gccgatcccc aaggttgcga
      841 catccgttcc gtcacgcaac acccgagccg tcccccgctc gacgaagtag aagtgttccc
      901 cgggctcgcc ctgcacacag acagcagccc cctgaacgac cgactccggc gcgatggtcc
      961 gtgccagacg ctccagctcg gccgggccga gggcggcgaa catgggggca gacctgagga
     1021 gatggaggtg ctcgatgggg acgatcaccc cgacatcggc ctcctgcagg gcgccgcggt
     1081 agaccaggag aaccatgatt atgagcaaac tcgcaacgac gagggcccac ccgacgccca
     1141 gccagccgat gaggacagcc cctgtcaccg agccgagtgc ctcggcggcc atcgaggtcc
     1201 cctccaggac tccaaacgcc cgggagagac caccgagcgg gatgaccctc tgcagcatga
     1261 tccgtcccga cacatcgaca aaggtccagc cggcgcccat gaccgcgagc gcgacaaggg
     1321 caactgcctg tgatccaaac acggggatca atgacacgga cacggcggtg agcagcagtc
     1381 cccgccgcat cgatggagcg aggtgtctct tcccgatcag gacgacggcc aggaccgacc
     1441 ctgtcgcggc gcctatgccg atggcggaat tgagccaggc ggccccggca tcctccgaac
     1501 caaacacctc gaaggcgagg acgatggtgg cgacctcgag gacaccggcg aggaagacga
     1561 cgctggtcag catgaggagg atcaccctgg gtcggggctc gcggaaggcg tcgaatccgt
     1621 ccacgatcat ctgaattgca ctctgatcgg tgccctggtc ggcagccgtg ctggtgcgga
     1681 cactccagac caggatcgtg ccgaggagga gaaggaccgt cgtgatgacg aacacggccc
     1741 cgattcccca ggcggccgca atgagtccgg cgaggagcgg cccagcgatc cagcccacgt
     1801 tctcgatggt cccaccagcg acataccctg ccgtgagttg ctcggtgctg cgagcgagag
     1861 cggggaggag ggactcgtga aacggtcgca ccagggtcaa taccgtggca ttgaccgcgg
     1921 cgaggagata tatccatccc acggaccagt cggcgtagag cgccaccgcc accgaggccg
     1981 acgaggcggc aaatacggca taggccacgg tgagcatgac ctcgcggcgg tagcggtcgc
     2041 caagcgtggc tcccaccggg gcgaacaggg cggccggggc gagttggagg aacgcgacca
     2101 gacccagggc cggggagcca ccca
//
LOCUS       DASTXO010000158         4898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1004225, whole genome shotgun sequence.
ACCESSION   DASTXO010000158 DASTXO010000000
VERSION     DASTXO010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004225"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..156)
                     /gene="rplE"
                     /locus_tag="VFU96_03355"
     CDS             complement(<1..156)
                     /gene="rplE"
                     /locus_tag="VFU96_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU5112327.1"
                     /translation="MSEQPRLKVLYAEQLAPRLKVELGLANVMEIPRLEKIVVNMGVG
                     EAVQDKKS"
     gene            complement(153..458)
                     /gene="rplX"
                     /locus_tag="VFU96_03360"
     CDS             complement(153..458)
                     /gene="rplX"
                     /locus_tag="VFU96_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU5112328.1"
                     /translation="MKLKHGDKVMVIAGKDAGKESRITRVIPDKGKVLVEGVNTAKRH
                     RRPTGSTMQGGIIDKDMPIDVSNVMIVCEKCGPTRIGHRIDDKGKKHRTCVKCGGDL"
     gene            complement(458..826)
                     /gene="rplN"
                     /locus_tag="VFU96_03365"
     CDS             complement(458..826)
                     /gene="rplN"
                     /locus_tag="VFU96_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003940951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEU5112329.1"
                     /translation="MIQQESRLKVADNTGAREVLCIRVLGGSSRRYAGVGDVIVAAVK
                     DANPGGSVKKGEVVKAVVVRTKKESRRPDGTYIRFDDNACVIIDNNSQPRGTRIFGPV
                     ARELRDRKFMRIVSLAPEVL"
     gene            complement(823..1089)
                     /gene="rpsQ"
                     /locus_tag="VFU96_03370"
     CDS             complement(823..1089)
                     /gene="rpsQ"
                     /locus_tag="VFU96_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014465728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU5112330.1"
                     /translation="MTDSNTRGRRKVRVGVVVSDARDKTVTVEVPNQMSHPRYDKVVR
                     RSTKFHAHDEANDAKLGDTVRIMETRPLSKQKRWRVVEVVERAR"
     gene            complement(1089..1316)
                     /gene="rpmC"
                     /locus_tag="VFU96_03375"
     CDS             complement(1089..1316)
                     /gene="rpmC"
                     /locus_tag="VFU96_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU5112331.1"
                     /translation="MTPSEFREMTDAELIEALAEAREEKFNLRFQLATNQLDNHARIK
                     AVKQDIARILTVLHERELTSVDATAGTEERA"
     gene            complement(1313..1726)
                     /gene="rplP"
                     /locus_tag="VFU96_03380"
     CDS             complement(1313..1726)
                     /gene="rplP"
                     /locus_tag="VFU96_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU5112332.1"
                     /translation="MLMPKRTKHRKVHRGRRSGVAKGGTHVAFGEYGLQALESGWITA
                     RQIESARVAITRAVKRGGKVWITIFPDKPVTQKPAETRMGSGKGNPEFWVSVVKPGRV
                     MFELAGVPEDLAREAMRKAGHKLPIKTRFVTREDS"
     gene            complement(2168..2788)
                     /gene="rpsC"
                     /locus_tag="VFU96_03385"
                     /pseudo
     CDS             complement(2168..2788)
                     /gene="rpsC"
                     /locus_tag="VFU96_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463475.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            complement(2788..3135)
                     /gene="rplV"
                     /locus_tag="VFU96_03390"
     CDS             complement(2788..3135)
                     /gene="rplV"
                     /locus_tag="VFU96_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEU5112333.1"
                     /translation="MRVRAQAKYVRHSPYKVRRVLDLVRGLPVHEAEDVLRLTSRGAT
                     EPISKVLKSAVANAEHNHALDADELIVAEAFADEGPTLKRYRPRARGRATRIRKRTSH
                     ITIVVSDTNEEVN"
     gene            complement(3132..3407)
                     /gene="rpsS"
                     /locus_tag="VFU96_03395"
     CDS             complement(3132..3407)
                     /gene="rpsS"
                     /locus_tag="VFU96_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEU5112334.1"
                     /translation="MSRSLKKGPFVDDHLANKVDELNRSGSKRVIRTWSRRSTIIPEM
                     IGHTIAVHDGRKHVPVYITESMVGHKLGEFAPTRTFRGHADKAARKR"
     gene            complement(3411..4244)
                     /gene="rplB"
                     /locus_tag="VFU96_03400"
     CDS             complement(3411..4244)
                     /gene="rplB"
                     /locus_tag="VFU96_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU5112335.1"
                     /translation="MAVKKRKPTSAGRRFQTVSDFAEITTDRPEKTLISKKGKTGGRN
                     TQGRVTSRHRGGGHKQRYRIIDFRRDKDGVPARVATIEYDPNRNARIALLNYADGEKR
                     YILAPVGVKVDDRLESGSGAEIRPGNALPLRNIPAGTNVHAIEMRPGGGAKLARSAGT
                     SVQLMSKENDLALLRLPSGEMRQVPLDCRATVGQVGNTEAELIKLGKAGRNRWKGVRP
                     QTRGVAMNPVDHPLGGGEGKSSGGRHPVSPWGQPEGRTRSKKKASNQYIVRRRAKKGR
                     R"
     gene            complement(4246..4533)
                     /gene="rplW"
                     /locus_tag="VFU96_03405"
     CDS             complement(4246..4533)
                     /gene="rplW"
                     /locus_tag="VFU96_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU5112336.1"
                     /translation="MKDPREVILAPVVSEKSYDLIERNNTYTFVVDTRTNKGEIKDAV
                     ESIFDVRVVRVNTLNRKGKMKRQGWTRGRRKDTKRALVTLADGNSIDLFGV"
     gene            complement(4533..>4898)
                     /locus_tag="VFU96_03410"
     CDS             complement(4533..>4898)
                     /locus_tag="VFU96_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL4 family ribosomal protein"
                     /protein_id="HEU5112337.1"
                     /translation="KKMKRLALRSALSARASEEAVMVIEDIDWSTPKTKQAVTLLEAI
                     GAGGKSLVVLGKLDGTAGRSFRNLPDVHLCEPGHLTPYDVLWADQVIFTSHTVGSVGR
                     SSSYEVSDSDFVKDEGSEA"
BASE COUNT          739 a         1618 c         1544 g          997 t
ORIGIN      
        1 cgacttcttg tcctggaccg cttcgcctac acccatgttg acgacgatct tctcgagacg
       61 cgggatctcc atgacgttgg ccagccccag ctcgaccttg agacgggggg cgagctgctc
      121 ggcgtagagc accttcaggc gaggttgctc gctcataggt cacctccaca cttgacacat
      181 gtccggtgtt tcttgccctt gtcgtcgatg cgatggccga tgcgggtcgg gccgcacttc
      241 tcacagacga tcatcacgtt cgagacgtcg atgggcatgt ccttgtcgat gatcccgccc
      301 tgcattgtcg agccggtggg gcgacgatgc cgcttggcgg tgttgacccc ctcgaccagg
      361 accttgccct tgtcagggat gactcgggtg atgcgcgact ccttgccggc gtccttgccg
      421 gcgatcacca tcaccttgtc cccgtgcttc aacttcatca caagacctcc ggagcgagcg
      481 agacgatccg catgaacttg cgatcacgaa gctccctggc caccggcccg aagatgcggg
      541 tcccccgagg ctgagagttg ttgtcgatga tcacgcacgc gttgtcgtcg aagcggatat
      601 aggtgccgtc ggggcggcgt gactccttct tggtgcggac gacgacggcc ttgaccacct
      661 cgcccttctt caccgacccg cccgggttgg cgtccttgac cgcagccacg atcacatcgc
      721 cgacgccggc ataccggcgg gacgagcccc cgagcacgcg gatgcagagc acctcgcggg
      781 cgccggtgtt atccgcgact ttgagccggg actcctgctg gatcatctgg ccctctccac
      841 gacttccacc acgcgccacc gcttctgctt cgacagaggg cgggtctcca tgatccggac
      901 cgtgtcgccg agcttggcgt cgttggcctc gtcatgggcg tggaacttgg tgctgcgacg
      961 gaccaccttg tcgtaacgcg ggtggctcat ctgattgggc acctcgacgg tgacggtctt
     1021 gtcacgggcg tcggacacga ccacgccgac acggaccttg cgtcgccctc tcgtattgct
     1081 atctgtcatc aggcacgctc ctcggtcccg gcggtggcgt cgacagaggt gagctcacgc
     1141 tcatgcagca cggtgagaat ccgggcgatg tcctgcttga cggccttgat gcgggcatgg
     1201 ttgtccagct gattggtggc gagctggaag cgaaggttga atttctcctc cctggcctcg
     1261 gccagcgcct cgatcagctc tgcgtctgtc atttcacgga attcggaagg cgtcatgagt
     1321 cctcccgggt cacgaatcga gtcttgatcg gcagcttgtg cccggctttc cgcatggcct
     1381 cgcgggcgag gtcctcgggg acgccggcaa gctcgaacat cacccgtcca ggcttcacca
     1441 ccgacaccca gaactctggg ttccccttgc ccgagcccat gcgggtctcg gcgggtttct
     1501 gggtaaccgg cttgtcgggg aagatcgtga tccacacctt cccgccacgc ttcacggctc
     1561 gggtgatggc gacacgggcg gactcgatct ggcgggctgt gatccagccc gactcgagcg
     1621 cctgcaagcc gtactctccg aacgccacgt gggtgccgcc cttggcgacg ccggagcgac
     1681 gtccgcgatg aaccttgcgg tgctttgtcc tcttgggcat caacacggcc tactcctcct
     1741 cctccgagct gacccgagtg ggctcgaccg cgtcggtatc cactgtcggc tcggcgggag
     1801 tctcgacgac gggctcgccg gcttgtgcag ccgaggtatc tccggtcggc tccgcggacg
     1861 tagcgccacc aggggcggtg acatcgacgt cttcgacggc cgactcgtac tcgaccttgg
     1921 cctcggccgc agtcggcctg cgacccccac cggcctcgat gagacgccgg ccgccctcct
     1981 tgttgccgcc gcctgcctcg atgagacgct tcccgccacc cgcttcgatg aggcgcggct
     2041 tgcgctcacc ggctccggca gcctcttcgg cccgtgactt gatgctcttc tgggtgcgtc
     2101 gcgccggccc accggtagcc aggtcggcct cggcggcgat acgatcgccg gagacctggc
     2161 cggtgctgac cacctcacct cggtagaccc agaccttcac tcctatcgcc ccgaccgtgg
     2221 tcttggccgt ggcagtcccg tagtcgatgt cggctcggag ggtgtgcagg ggcactcggc
     2281 cctcgcggta ccactcgcga cggcccatgt cggcaccgcc gaggcgcccc ttgcactcga
     2341 cccggacgcc ctcggccccg gccttcaggg cagacgcgat ggcacgcttc atggcgcggc
     2401 ggaaagcgat gcggttctcg atctggtcgg caaccccgcg ggccagcagg gcggcatcta
     2461 gatcaggatc cttgacctcg ttgatgttga gcttcaccgg ccgtttggcc agggcctcga
     2521 gatcgccgcg caagcgctcg gcttcggcgc ccttgcgacc gatcacgacg ccggggcgag
     2581 cggtgtggat gtccacgatg agacggtcac gggtgcgctc gatatcgact ctcgatacgg
     2641 cgccgcggac cagctccctc ttcagataat cgcggatccg ccagtcctcg ttgacgaagt
     2701 cgacgtattc cttgtcagag aaccacttcg acttccagtc agtggaaaca ccgaggcgga
     2761 atgagtaggg gtggaccttc tgacccatca gttcacctcc tcattcgtgt ccgacaccac
     2821 gatcgtgatg tggctggtgc gctttcggat tcgggtggcc cgtccccggg cccggggccg
     2881 atagcgcttg agggtcggtc cctcgtcggc aaaggcctcg gcgacgatca gttcgtcggc
     2941 atccagagcg tggttgtgct ctgcattggc cacggccgac ttcaacacct tgctgattgg
     3001 ctctgttgcg ccgcgcgagg tcagccgaag cacatcttcg gcttcatgca cgggcaggcc
     3061 gcgcaccagg tcgagcacgc gtcgaacctt gtacggcgag tgccgcacgt atttggcttg
     3121 tgctctgact ctcatcgttt cctcgctgcc ttgtcggcgt gtccccggaa ggtgcgggtt
     3181 ggggcgaact cacccagctt gtgacccacc atcgattcgg tgatgtacac cggcacgtgc
     3241 ttgcgaccgt cgtggacagc gatcgtgtgc ccgatcatct ccgggatgat cgtggagcgc
     3301 cgggaccagg tcctgatcac ccgcttgctt cccgagcggt tcaactcatc caccttgttg
     3361 gccaggtggt cgtcgacgaa cgggcccttc ttaagacttc tgctcatttg ttatctgcgg
     3421 cccttcttgg cgcgacgacg gacgatgtac tgattggacg ccttcttctt gctccgggtc
     3481 cggccctccg gctggcccca tgggctcacc gggtgacggc cgcctgaaga cttgccctct
     3541 ccaccaccca gcgggtgatc gaccgggttc atggcgacac ctctggtctg gggacgaact
     3601 cccttccagc gattgcggcc ggccttgccc agcttgatca actcggcctc ggtgtttccc
     3661 acctgaccga cggttgcccg gcaatcgaga ggcacctggc gcatctcacc ggagggaaga
     3721 cgaagcagcg cgaggtcgtt ttccttcgac atcagctgca ccgaggttcc ggcggagcgg
     3781 gcgagcttgg ctcccccgcc ggggcgcatc tcgatggcat gaacgttggt gccggcagga
     3841 atgttccgca aaggcaaggc attcccgggg cggatctcgg caccggagcc cgattcgagg
     3901 cggtcatcga ccttcacccc gaccggggcc aggatgtacc tcttctcgcc gtcggcgtaa
     3961 ttcagcaagg cgatgcgggc attgcggttg gggtcgtatt cgatggtggc gacgcgcgcg
     4021 ggcacgccgt ccttgtcacg acggaagtcg atgatccggt atcgctgttt gtggccaccg
     4081 ccacgatggc gagaggtgac tcggccctgg gtgttacgac cgccggtctt gcccttcttg
     4141 gagatcaacg tcttctcggg cctgtccgtg gtgatctcgg cgaagtccga gacggtctgg
     4201 aacctgcgac cagctgaggt cggcttgcgc ttcttgactg ccatctcaga ctccgaacag
     4261 atcgatcgaa ttgccgtcgg caagagtgac gagggcgcgc ttggtgtcct tgcggcgacc
     4321 acgcgtccat ccctggcgct tcatcttgcc cttgcgattg agtgtgttga ccctgaccac
     4381 acggacgtcg aagatcgact ccacggcgtc cttgatctca cccttgttgg ttcgggtgtc
     4441 gaccacgaag gtgtacgtgt tgttgcgctc gatgaggtca taggacttct ccgagacgac
     4501 cggggcgagg atcacctcgc gtgggtcctt catcaggcct cactcccctc gtctttgacg
     4561 aagtcgctgt cgctgacctc gtacgagctg gaccggccca ccgaccccac cgtgtgcgag
     4621 gtgaagatga cctggtccgc ccagaggacg tcgtagggcg tgagatggcc cggctcgcag
     4681 agatggacat cgggaaggtt ccggaaggaa cggccggccg tcccgtcgag cttgccgagc
     4741 accacgagtg acttgccacc ggcgccgatg gcctcgagca aggtcaccgc ctgcttggtc
     4801 ttgggggtgg accagtcgat gtcctcgata accatgaccg cttcttcgga ggcgcgggcg
     4861 gagagggcgc tgcggagggc aagacgcttc atcttctt
//
LOCUS       DASTXO010000159         6088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_489110, whole genome shotgun sequence.
ACCESSION   DASTXO010000159 DASTXO010000000
VERSION     DASTXO010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6088
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489110"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1415)
                     /locus_tag="VFU96_03415"
     CDS             complement(<1..1415)
                     /locus_tag="VFU96_03415"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112338.1"
                     /translation="MVAPLEPLLSGWRSAPLPDLHGTWSVAPGGRAFAIAGLADQLES
                     ALLVVVPGEGDAEELVDDISLFIDNVLLAPAWETLPFEHVSPNIGTMARRAEARHALE
                     RGGRTVVVTSVRGAIQRLSPSPHVPLILSPGDEVTMDALSRALADLGYSRTDRVEARG
                     EFAVRGGIVDVFPAQADSPVRLDFWGDQLEETRAFSVASQRSEDAVPRLIAFPAREVR
                     PDLLIRDRAEQLVRSEPWSASTWDRIGQGQIFPGIESWLPWLAEPVSMIDAMPGDAAL
                     VLVEPSRALDRARELVKEESELAAALAPTWGDQAPIAGEHPALFLDLEESLTGRPHLR
                     MPASPMSPDDRVLEISALDAIPGSPESVAAGVGRLLARRIDTVIAMDGEAAADRVAAA
                     LAEQGLALDRLSELETPRSAVIPLGIHKGFVVPSIGVAVLGEQEIAGRRRAHRRAGRA
                     RTEVGQQYQDLTEGDFVVHHQ"
     gene            complement(1440..2411)
                     /locus_tag="VFU96_03420"
     CDS             complement(1440..2411)
                     /locus_tag="VFU96_03420"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU5112339.1"
                     /translation="MRVGVTGGSGVVGRALVRHLIDAGHQVVALARTPDSAADLRVRG
                     ATPVDGDVLDPPGLRALVEGKDWVFHVAGINEMCSPDPDLMDLVNIEGTRNVMEACRS
                     AGVGRMIHTSSAAAIGEAHGTIGSESTPHRGSYLSRYERSKHLAERVLFDQAGDLEVV
                     AVNPSSVQGPGRATGTGKLILDLVNGRLPFLVETTVSMVDIDDCARGHVLAAERGRFG
                     QRYILNGSSSSISEVVDLFNAITGRDLAPRFVPGWMASGGAAAIEIGGRIGGRRPPVC
                     REMVRVLRAGHTYDGSRATRDLGLEYTPLESTIARTVDWFRAQGLLV"
     gene            complement(2408..2965)
                     /gene="pgsA"
                     /locus_tag="VFU96_03425"
     CDS             complement(2408..2965)
                     /gene="pgsA"
                     /locus_tag="VFU96_03425"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEU5112340.1"
                     /translation="MSTPDILALSRILLVPFIMAFTRSDRIDNALVIAATLFAVAAFT
                     DFLDGYLARKTSGGTMLGAFLDTTADKILITGTLLALVSIDRVSIWPASIIIFREFLV
                     MALRGVVATQGGLIRPSTWGKIKFGTQFLAIFLAYFRLADRWGPWYLDQWVMLIAVIA
                     TVGSAWGYISAFWHTVRSEPTPTRT"
     gene            complement(2981..3364)
                     /locus_tag="VFU96_03430"
     CDS             complement(2981..3364)
                     /locus_tag="VFU96_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112341.1"
                     /translation="MAEHLGSTKGYLDDEALDDYLTCFRDPETIRGMCEDYRAGATID
                     YEIDLADMEAGARIHCPVLVLWAAKGELEEWYDPLEVWREWAEDVRGGALPCGHYLAE
                     EAPEETVEALMAFLSEPAPPIRSDR"
     gene            complement(3301..3882)
                     /locus_tag="VFU96_03435"
     CDS             complement(3301..3882)
                     /locus_tag="VFU96_03435"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5112342.1"
                     /translation="MFEGFDLEMLDLDEIVVRVRIGGAGPPLLLLHGAKQTHVMWHLV
                     APALAERFTVIAPDLRGYGRTSKPPSTPDHFNYSKRVMARDQVDLMSALGHSKFSVVG
                     HDRGGRVAYRLALDHPGAVRSLGVLDIIPTGEAFERGGREFGLGFYHWFFLAQPTPFP
                     EKLIGADPEWYWRWPSIWALRRAISTMRPWTTI"
     gene            complement(3885..5363)
                     /gene="glpK"
                     /locus_tag="VFU96_03440"
     CDS             complement(3885..5363)
                     /gene="glpK"
                     /locus_tag="VFU96_03440"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEU5112343.1"
                     /translation="MGALDQGTTSTRFMIFDHDGSVIAADQREHRQIFPQPGWVEHDP
                     VEIWSNTTLVIEGALSHAGLKPSDLAAVGLTNQRETTVLWDRTTGEPVHNAIVWQDTR
                     TAELCVELAGDAGPDRFRAKTGLPLATYFSGPKARYLIDRLDLGQRAERGELAFGTID
                     SWIAWKLSGGTDGGIHVTDVTNASRTLLMDLATTTWDADLSLKIGVPTSILPEIVPSI
                     GTEIECIGPLAGVRLAAILGDQQAALFGQACFEPGEAKNTYGTGNFMLLNTGEIPVAS
                     DAGLLTTVAYRVAGQRPVYALEGSVAVTGSLVQWLRDNLRLFDDASEVEDLARSVEDN
                     GDVYFVPAFSGLFAPHWRPDARGVIAGLTRFANRGHIARATLESTAFQTRDVLDAMVA
                     DSAVDLKELRVDGGMTSNSLLMQFQADILGVDVILPRVAETTALGAAYGAGLAVGYWS
                     GLDEVRSLWSEDARWSPAMSVEERDRLHRRWQQAVERSLGWV"
     gene            complement(5384..>6088)
                     /locus_tag="VFU96_03445"
     CDS             complement(5384..>6088)
                     /locus_tag="VFU96_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014442004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEU5112344.1"
                     /translation="VLASLGFEAIATTSSGHAASLGRMDQEVTLEELLAHVDALVGAV
                     DVPISVDAERCFADDSNRVSANVTQIAGTGAAGVSIEDYDPGSGIDPVDIAIERVAAA
                     AEVTRSHGMVLTARAENHLYGIEDLDDTIARLRAYRQAGADVVYAPWLADIGQISRVV
                     SETASPVNVLARVDGPDVGELAAVGVRRVSTGGALAFAAYGALAKAARELLSEGTSSY
                     IRRGLSMDDRRAAFQR"
BASE COUNT         1100 a         2053 c         1964 g          971 t
ORIGIN      
        1 tgctgatgat ggacgacgaa gtcgccttcc gtcaggtcct ggtactgctg gcctacctcg
       61 gtccgggccc gacccgccct gcgatgggca cggcggcgcc cggcgatctc ctgctcgccc
      121 agtacggcca ctccgatgga tggcacgacg aagcccttgt gtatcccgag cgggatcacc
      181 gccgacctcg gtgtctccag ctctgacaga cggtcgagcg ccaggccctg ttcggccaag
      241 gccgccgcca cgcggtcggc cgcggcttcg ccgtccatcg cgatcacggt gtcgatccgc
      301 cgggccagga gtctccccac cccggcagcg acagactccg ggcttcccgg gatggcatcg
      361 agagcgctga tctcgaggac ccggtcgtcg gggctcatgg ggctcgcagg cattcgcagg
      421 tggggccgac cggtgagact ctcctccaga tcgaggaaca gcgcggggtg ctcacccgcg
      481 atcggcgcct gatcgcccca cgtcggggcc aacgcggccg ctagctcgga ctcctccttg
      541 accagctctc gggcccggtc gagtgcacgg ctcggctcga ccagcaccaa agcggcatcg
      601 ccgggcatgg cgtcgatcat cgatacgggc tctgcgagcc agggaagcca ggactcgatc
      661 ccggggaaga tctggccctg cccgatgcgg tcccacgtgg aggcactcca tggctcgctc
      721 cgaaccagtt gctcggcccg atcgcgaatc agcagatccg gtcgcacctc gcgggcaggg
      781 aacgcaatca gacggggcac ggcgtcctcg gagcgttggc tcgccaccga gaaggccctg
      841 gtctcctcga gctgatcgcc ccagaagtcg agccgcacgg gactgtccgc ttgagccggg
      901 aacacgtcga cgatcccccc tctgaccgcg aactcgccac gcgcctcgac ccggtcggtg
      961 cgggaatagc cgaggtcggc gagggcacgg gatagggcgt ccatggtgac ctcgtctcct
     1021 ggcgacagga tcagaggcac gtgaggcgat gggctcagcc gttggatcgc cccgcggaca
     1081 ctggtgacga ccacagttcg gccgccccgc tccagggcgt gtctcgcctc agcgcgacgc
     1141 gccattgtgc cgatgttcgg tgagacgtgc tcgaacggca gcgtctccca tgccggggcg
     1201 agcaggacgt tgtcgatgaa cagggagatg tcgtcgacca actcctcagc atctccctcc
     1261 ccgggcacga ccacgaggag tgctgactcg agttggtcgg cgagacctgc aatcgcgaag
     1321 gcgcgccctc ccggggccac ggaccaggtg ccgtggagat cgggaagtgg ggcggaccgc
     1381 cagcctgaaa ggagtggctc gaggggggca accatcgggc agccgatggt acctgtggtt
     1441 cagaccagca ggccctgtgc gcgaaaccag tccacggtgc gagcgatcgt gctttccagc
     1501 ggcgtgtatt cgagacccag gtcgcgggtt gcgcggcttc cgtcgtaggt gtggcccgcc
     1561 cgcagcaccc tgaccatctc acgacagact gggggacgcc ggccgccgat gcgtcctccg
     1621 atctcgatcg cggccgcccc tccagaagcc atccagccgg gcacgaatct cggagccagg
     1681 tcacgacccg tgatcgcgtt gaacaggtcg accacctccg atatggaaga gctggagcca
     1741 ttgaggatgt agcgctgccc gaaccttcca cgttcggcag cgagcacgtg accgcgtgca
     1801 caatcgtcga tgtcgaccat cgacacggtc gtctccacca ggaacgggag cctcccattc
     1861 acgaggtcga ggatcagctt ccccgtcccc gtcgcccggc cgggtccctg gaccgacgat
     1921 gggttgaccg ccaccacttc gaggtcgccg gcctgatcga acaagacacg ctcggcgagg
     1981 tgtttggacc gctcgtatct ggacagatag ctcccccgat gtggcgtgct ctcggagccg
     2041 atggtcccgt gcgcctcacc tattgcagca gccgaagagg tgtggatcat cctcccgacc
     2101 cccgcggagc ggcaagcctc catgacgttg cgagtcccct cgatgttcac cagatccatc
     2161 agatccgggt ccggcgagca catctcgttg atcccggcga catggaacac ccagtccttc
     2221 ccctcgacca gcgcccgaag accgggaggg tcgagcacgt cgccgtcgac cggtgtcgct
     2281 ccccggaccc gcaggtcggc agcagaatcc ggagttcgag ccagggcgac gacctgatgc
     2341 ccggcgtcga tcagatgacg gacgagggcg cgacctacga caccgctgcc gccggttacg
     2401 ccgaccctca cgtccgggtc ggggtcggct ccgagcgaac cgtgtgccag aaggcggaga
     2461 tgtaacccca ggcggaccca accgtggcga tgaccgcgat gagcatcacc cactggtcga
     2521 ggtaccacgg cccccagcga tcggcgagcc tgaagtaggc gaggaagatg gcgaggaact
     2581 gggtgccgaa tttgatcttg ccccatgtcg acgggcgtat gagaccacct tgagtcgcca
     2641 ccacgccgcg cagcgccatg accaggaatt cgcgaaagat gatgatcgag gccggccaga
     2701 tggagacccg atcgatcgag accagggcga gcagggtgcc cgtgatgagg atcttgtcgg
     2761 cggtggtgtc gaggaaggcg ccgagcatgg tgcccccgct ggtcttgcgg gccaggtagc
     2821 cgtcgaggaa atcggtgaac gcagcaaccg cgaagagagt ggctgcgatg acgagagcgt
     2881 tgtcgatccg gtcggaccgg gtgaacgcca tgatgaatgg caccagaagg atccgggaga
     2941 gcgccaggat gtcaggcgtt gagatcgggg tcctcctcga ctaccgatcc gatcttatag
     3001 gtggcgccgg ttcagagagg aaggccatga gcgcctcgac cgtttcctcc ggagcctcct
     3061 cggcgagata gtgcccgcat ggaagagccc cgccccgaac atcctcggcc cactcgcgcc
     3121 atacctcgag cgggtcgtac cactcctcga gctcaccctt cgccgcccaa aggacgagca
     3181 ccgggcaatg gatcctcgct ccagcctcca tgtcggccag gtcgatctca tagtcgatgg
     3241 tggccccggc ccggtagtcc tcgcacatgc cacgaatcgt ctccggatcc cgaaaacaag
     3301 tcagatagtc gtccagggcc tcatcgtcga gatagccctt cgtagagccc aaatgctcgg
     3361 ccacctccaa taccactcag gatctgctcc gatcagcttc tccgggaacg gagtcggctg
     3421 ggcgagaaag aaccagtgat agaagccaag cccgaattca cgcccgcctc gctcgaatgc
     3481 ctccccggtg gggatgatgt cgagtacccc caagctgcgg acagcacccg ggtggtccag
     3541 agccaaccgg taggcgacac ggccgccccg atcatggcca acgaccgaga acttcgagtg
     3601 gcccagcgcc gacatgaggt cgacctggtc acgcgccatc acgcgcttcg agtagttgaa
     3661 atggtccggg gtggagggtg gcttggaggt cctcccgtag ccacggaggt cgggggcgat
     3721 caccgtgaat cgttcggcga gggccggggc aaccaggtgc cacatgacat gggtctgctt
     3781 cgctccatgg agaaggagca gcggcgggcc ggctccccca atccgcaccc gcacgacgat
     3841 ctcatccagg tcgagcatct cgagatcgaa tccctcgaac atgctcagac ccagcccaat
     3901 gatctctcca ccgcctgctg ccagcgacga tggagccgat cacgttcctc gacgctcatc
     3961 gccggggacc aacgggcatc ctcggaccac aggctccgca cctcgtcgag tcctgaccaa
     4021 tacccgacag cgagaccggc cccataggcg gcgccgaggg ctgtggtctc cgcgacccgg
     4081 ggcaggatca cgtccactcc gaggatgtcc gcctgaaatt gcatgagcag gctgttggac
     4141 gtcatcccac cgtcgacccg gagctccttt aggtcgacgg ccgagtcggc caccatcgcg
     4201 tcgaggacat cacgggtttg aaacgcggtg gattcgagcg tggcgcgggc gatgtgtccc
     4261 cgattggcga accgggtgag cccggcgatc accccccggg cgtccggcct ccagtgcggc
     4321 gcgaagagac cggagaacgc cgggacaaaa tagacgtcgc cattgtcctc gaccgagcgg
     4381 gcgaggtcct cgacctcgga ggcgtcgtcg aacaggcgca gattgtcccg aagccactgg
     4441 accaacgaac cggtgacggc aacggagcct tcgagggcgt agaccgggcg ctgcccggcg
     4501 acccggtagg caaccgttgt caagagtccg gcatctgagg cgaccgggat ctcccccgtg
     4561 ttgagcagca tgaaattccc cgtgccatat gtgttctttg cctcgcccgg ctcgaaacac
     4621 gcctgaccga acagggccgc ctgttggtca cccaggatcg ccgccagacg gactccagcc
     4681 agcggcccga tgcactcgat ctcggttccg atcgagggga cgatctcggg gagaatggaa
     4741 gtcggcacac cgattttgag gctgagatcg gcgtcccagg tcgtcgtggc caggtccatg
     4801 aggagcgtgc gcgaggcatt ggtgacgtcg gtgacatgaa ttcctccgtc ggtgccgccg
     4861 ctgagtttcc aggcgatcca ggagtcgatc gtcccgaagg ccagttcccc ccgttcagcg
     4921 cgctggccga gatcgagacg atcgatcaga tagcgggcct tgggcccgga gaagtaggta
     4981 gccaggggaa gcccggtctt cgccctgaat cggtcaggcc ccgcatcgcc cgccagctcg
     5041 acgcataatt cggcggtacg tgtgtcctgc cacacgatgg cgttgtgcac gggctcaccg
     5101 gtcgtgcggt cccacaacac cgtcgtctcc ctttgattcg tgagtccaac cgccgcgagg
     5161 tcggatggct tcagtccggc gtgggagagg gcgccctcga tgaccaatgt cgtgttcgac
     5221 cagatctcga ccgggtcgtg ctccacccac cccggctgag ggaagatctg gcgatgctct
     5281 cgctgatcgg cggcgatgac cgatccgtcg tggtcgaaga tcatgaaacg ggtcgatgtc
     5341 gtcccctggt cgagggcgcc gatgagatcg ggcacgacga tccctaccgt tggaacgcgg
     5401 cccggcgatc atccatcgaa agccctcgcc ggatgtagct ggacgtgccc tccgacaaca
     5461 actccctggc cgctttggcc aacgccccat aggcggcgaa ggccaacgca ccgccggtcg
     5521 agacccgcct gacaccgacc gcggccagtt ctccaacgtc tgggccatcg acccgggcca
     5581 ggacattcac cggcgacgcc gtctcggata cgacgcgtga gatttggccg atatcggcca
     5641 gccatggcgc gtagacgacg tctgctccgg cctggcgata agccctcagc cgggcaatcg
     5701 tgtcgtccag atcctcgatc ccgtagagat ggttctcggc cctcgcggtc agcaccatcc
     5761 cgtgcgatcg ggtcacctcg gccgcggccg ccacccgttc gattgcgatg tcgaccgggt
     5821 cgatcccgga gccagggtca tagtcctcga tcgacacccc ggcggcaccg gtgccggcga
     5881 tctgtgtcac gttcgcggac acacggttgg agtcgtccgc aaagcatcgc tcggcgtcga
     5941 cgctgatagg cacgtcgacc gcccccacca atgcgtccac gtgagccagc agttcctcga
     6001 gcgtgacctc ctgatccatc cgccccagcg aagcggcgtg ccctgagcta gttgtggcga
     6061 tcgcctcgaa cccgagcgat gcgagaac
//
LOCUS       DASTXO010000160         2206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_83594, whole genome shotgun sequence.
ACCESSION   DASTXO010000160 DASTXO010000000
VERSION     DASTXO010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83594"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1144
                     /locus_tag="VFU96_03450"
     CDS             <1..1144
                     /locus_tag="VFU96_03450"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEU5112345.1"
                     /translation="VVPAAATNQAAPQQGASITSQSFGVIDADGHPEDGKEVFLYTLT
                     NRKGMRVNILTYGGILQSIEVPGRRGRLTNVTLGFNNLEQYVNQNPYFGNITGRYANR
                     IALGQFTLNGQDYQLATNNDPNHLHGGDFGFDKRIWAAEEIDDGRNVGLRLTYTSPDG
                     EESYPGTLHVTVTYLLTPKNEIRMDYRAELDEAESFSTIVNLTNHAYFNLAGEGNGDI
                     YDHKLYINADEYTPVDETLIPTGAIDPVAGTPMDFNRPTAIGDRINDGTFEQLVIGRG
                     YDHNWVLDRVTDDRLELAARATDPESGRTLKVLTTEPGIQFYAGNFLDGTLIGTSGKM
                     YRQSYGFALETQHFPDSPNHPSFPSTVLVPGEVYETTTIYAFGNNR"
     gene            1267..>2206
                     /locus_tag="VFU96_03455"
     CDS             1267..>2206
                     /locus_tag="VFU96_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112346.1"
                     /translation="MNAYITGVGSYLPGEPVGNDEIESYLGRSCGSDRIRRRMLEANG
                     IEKRHYAIDREGRTQMLNEELAEAAVRAALQTRGMTPGDIGLLATGTTQGDLPVPGFA
                     SMVHGRLGGGPMEVLSAGGVCCSGIAALRAAEASVLLGRHRVAVAVGSEMVSRTLRAS
                     RFPDQGSFDAEFLRFMLSDGAGAVVIEDRPRSDGLSLRIDWSHLVSHAHRFPVCMYAG
                     SATPETVTAGATWQDQPTPADAHSAGMLNLRQDTAILGNIVALGVEEYIGLVRNGRIA
                     PREVDHLLVHFSSEYFRSDIVRLMGEAGLMIPEDRWF"
BASE COUNT          446 a          706 c          669 g          385 t
ORIGIN      
        1 ggttgtgccc gccgcggcca ccaatcaggc cgccccccag caaggagcaa gtatcaccag
       61 tcagtcattc ggtgtcatcg acgccgacgg tcatccggag gatggcaagg aagtcttcct
      121 atacaccctc accaaccgca aggggatgag ggtgaacatc ctcacctatg gggggatcct
      181 gcagtcgatc gaggtcccgg ggcggcgggg gcgtctcacc aacgtcaccc ttggtttcaa
      241 caacctcgag cagtatgtca accagaaccc ctacttcggg aacatcaccg ggcgttatgc
      301 caaccggatc gccctggggc agttcactct gaatggccag gactatcagc tggccaccaa
      361 caacgacccc aaccatctgc acggaggcga cttcggattc gacaaacgga tctgggcggc
      421 agaggagatc gacgacggga ggaatgtcgg gctcaggctc acctacacca gcccggatgg
      481 tgaagagagc tatccaggga cgctgcatgt caccgtgacc tatctgctga cgcccaagaa
      541 cgagatccgg atggattacc gggcggaact ggatgaagcc gagagcttct cgaccatcgt
      601 caacctcacc aaccatgctt acttcaacct ggcgggcgaa ggcaatggtg acatctacga
      661 ccacaagctc tatatcaacg ccgatgagta cacaccggtt gacgagacct tgatccccac
      721 cggggcgatc gacccggtcg ctggaacgcc gatggacttc aaccgaccca ccgctatcgg
      781 ggaccggatc aatgacggca ccttcgaaca gctggtgatc ggtcgtggtt atgaccacaa
      841 ctgggtgctc gaccgcgtta ccgacgatcg gctggagttg gccgcccgag ccacggatcc
      901 cgagagtggt cgcaccctga aggtgttgac caccgagccg gggatccagt tctacgccgg
      961 gaacttcctc gatgggaccc tgatcgggac cagcgggaag atgtaccggc aaagctacgg
     1021 gtttgccctc gaaacccaac acttcccgga ctcaccgaac catccgagtt tcccctcgac
     1081 agtgctcgtc cccggcgagg tgtacgagac aaccactatc tacgccttcg gcaacaaccg
     1141 ctgacctaca ggcccaccgc cgacgacgtc tttcttggag gtgatagccg gctccatcgg
     1201 ggtgaacccc gataccggcg cgctgtcccg ggaccgatcc tgacccatcg aaggacaagg
     1261 agggtcatga acgcatacat caccggtgtc ggctcctatc tgcccggcga gccagttggc
     1321 aacgacgaga tcgagtcgta tctgggcagg agctgcggat cggatcggat tcgacgccgg
     1381 atgctcgaag ccaacgggat cgagaagcgc cactacgcca tcgatcgcga gggccggacc
     1441 cagatgctca acgaggagct agccgaggcg gcggtccgag ccgccctgca gacgagaggc
     1501 atgactcctg gagacatcgg gttgctggcc accgggacca ctcaaggaga cctgcctgtc
     1561 ccaggtttcg cctcgatggt gcatgggcgc ttgggaggcg ggccgatgga agtgctctcc
     1621 gccggaggtg tctgttgctc gggtatcgcc gcactgcgcg cagccgaagc ctcagtgctc
     1681 cttggtcgac atcgcgtggc ggtggccgtc ggttcggaga tggtcagtcg cacactccgg
     1741 gccagtcgtt ttccggatca aggctctttc gacgccgagt tcctccgttt catgctgtca
     1801 gacggagccg gtgccgtggt catcgaggac cgaccccgca gcgatgggtt gtcgcttcgc
     1861 atcgactggt cccacctcgt ttcccatgcc caccggttcc cggtctgcat gtacgccggc
     1921 tcggcaaccc ctgagacagt gactgccggg gcgacctggc aggatcagcc aactccagca
     1981 gacgcgcact ccgcgggaat gctcaacctg agacaggaca ccgcgatctt gggcaacatc
     2041 gttgccctgg gcgtcgagga gtacatcggt ctggtgcgta acggccggat cgccccccgc
     2101 gaggtcgatc acctcctcgt gcacttcagc tccgagtact tccgctccga catcgtcagg
     2161 ctgatgggcg aggcggggct gatgatcccc gaggatcgat ggttca
//
LOCUS       DASTXO010000161         5761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1447108, whole genome shotgun sequence.
ACCESSION   DASTXO010000161 DASTXO010000000
VERSION     DASTXO010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5761
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447108"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2385)
                     /locus_tag="VFU96_03460"
     CDS             complement(<1..2385)
                     /locus_tag="VFU96_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5112347.1"
                     /translation="MDPLGAFTPATRTWFEASFNEPTQAQALGWPAIADGHHTLIHAP
                     TGSGKTLAAFLWTLDRLLTEPQPDKASRCRVLYISPLKALAHDVERNLRAPLAGIRHA
                     SSRIGGPTLPTVDTFIRTGDTPPDARQRMRRNPPDILITTPESLYLMLTSAAREVLRS
                     VRWVIVDEVHAVAGSKRGAHLSLSLERLEEVTDVSPQRIGLSATQRPLEVIGRYLGGG
                     TIGDDGIWEPRPVTLIDVPHDRDLDIQIVVPVEDMNEPGEPDPLDPDQQSTRSIWPAV
                     YPRLLEQIQAHRSTIVFANSRRLAERLCSEINNLAGEEVAKAHHGSVAREQRLLTEEA
                     LKRGDLKAVVATSSLELGIDMGAVDLVLQVEAPTSVASGLQRVGRAGHQVGGTSKARI
                     YPKYRGDLLVSAVVANRMLERRVETTRIPANPLDVLAQQLVARVVAGASSADALFEMT
                     IRAAPYSGLSRAVFDETLDMLAGRYPSDLFAELRPRINWDRATGEVSARPGALQLAVA
                     NAGTIPDRGLFRVTLPDGSRVGELDEEMVYESREGDVFLLGSTAWRISQIGHDRVEVI
                     PAPADGAARMPFWHGDSMGRSMETGKAVGAFVREMGRFDPDQAHDQLTSRFHLDDLAA
                     RNLVGYLAEEREATGVLPTDQTLVVERFRDEIGDWRLVLLSPLGARIHAPWAMALRHR
                     FRERYGADVDAIWSDDGIAFRFPDADNPPGVEDLLLDPEDVEALLLEHLGDTALFAAR
                     FREAAGRSLLLPRRRPGERTPLWLQRRRSADLLGVAKQFGSFPIMLEVYREILQD"
     gene            complement(2447..3022)
                     /locus_tag="VFU96_03465"
     CDS             complement(2447..3022)
                     /locus_tag="VFU96_03465"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEU5112348.1"
                     /translation="MRELPRDILIEVMEDVIEDLHGHVHRVSDLAVNLGIGIGLGEID
                     LDRLALAGVLHDVGKIHLDPGILGKPGPLDATERELMNRHPELGFAMTRNRLDPKVAE
                     AILYHHERYDGRGYPFGLAAEEIPILSRIILVADAFDAMTSTRAYQRALPVEFAVTEI
                     RKHAGSQFDPHVVDLFLELAAINRLPLELSA"
     regulatory      3183..3265
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            3325..4611
                     /locus_tag="VFU96_03470"
     CDS             3325..4611
                     /locus_tag="VFU96_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HEU5112349.1"
                     /translation="MRDRDFGFDTRQIHAGQRPDPETGARAMPIYATTSFVFEDAQQA
                     ADLFSLQNFGNIYTRIGNPTNAAFEERMASLEGGLGALATSSGQAAQLISIFTLCNAG
                     DEIVASRSLYGGTFTQFDVTLRRMGINVTFVDIHDHGAVKEAITPKTKALYGETIGNP
                     AADILDISAIADIAHDSGIPLIVDNTFATPYLCRPIEHGADIVVHSATKFIGGHGVVI
                     GGVIVESGLFPFDNGNFPGITEPSPAYHDLMFWENFREYAYLMKARVESLRDIGAAMS
                     PFNAFIMLLGLETLSLRMSRHTQNARRVAEFLFEHPDVAWVRYPIFPNNRWTESAQKY
                     VPDGPGAVFTFGVKGGFDQAANFIAGVELASHLANVGDAKTLVIHPASTTHQQVPVEE
                     RRAAGVGDEMIRISVGLEDIDDILWDLDQALEGARE"
     gene            4620..5090
                     /locus_tag="VFU96_03475"
     CDS             4620..5090
                     /locus_tag="VFU96_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008358767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU5112350.1"
                     /translation="MHEASALERLQIIQSARSVALVGVSANILRSSNFVATYLVRTDL
                     DMYPVNPMYDEILGLKSYPSLADLPVVPDIVDVFRRHDELPNVVDEAIAVGAKVVWFQ
                     LGLRHDEAAAKAAAAGLQVVQDRCLKIEHARFSGKLHLAGFNTGVISSRRLRPL"
     gene            complement(5092..>5761)
                     /locus_tag="VFU96_03480"
     CDS             complement(5092..>5761)
                     /locus_tag="VFU96_03480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HEU5112351.1"
                     /translation="DAGFPALLVENFGDVPFHAEASDPETVAAMSLAARAVSDATGVP
                     FGVNVLRNDALSALGIAVVTGAAFIRVNVLTGVMYTDQGPIVGRADELARRRAALAPE
                     IEIWADVMVKHATPPPGLDIRQAAEDTVIRGLADAVIVSGAGTGLEPDLHEAAMARSV
                     IPKGTRLVIGSGAAVENLGLLVEVADTVIVGSSLKADGTANSPLDMARVAPFIEAARH
                     HGLV"
BASE COUNT         1029 a         1820 c         1928 g          984 t
ORIGIN      
        1 gtcttggagg atctcccggt atacctcgag catgatcggg aaggagccga actgtttggc
       61 cacaccgaga agatcagccg atcgtcggcg ctggagccag agcggtgtcc gctcgccggg
      121 tcggcgtcgg ggcaggagaa gggagcgccc ggccgcctcc cgaaagcggg cggcgaagag
      181 agcggtgtca ccgagatgct cgagcaacag agcctccacg tcctcgggat cgagaaggag
      241 gtcctcgacg ccaggcgggt tgtccgcatc agggaagcgg aaggcgatgc cgtcatccga
      301 ccagatggcg tcgacatcgg ctccatagcg ttcacggaag cgatgacgaa gcgccatcgc
      361 ccacggagcg tggattcgag cgccgagcgg cgagagcagg accagcctcc agtcgccgat
      421 ctcgtcccgg aacctctcga cgacgagggt ctgatcggtg ggcaggactc ctgttgcctc
      481 ccgttcctcg gcgagatatc cgacgagatt ccgggccgcc aggtcgtcga ggtgaaagcg
      541 tgaggtcagc tgatcgtgcg cctggtcggg atcgaatcgt cccatctccc gaacgaaggc
      601 acccaccgcc ttgccggtct ccatggaacg ccccatcgaa tcgccgtgcc aaaacggcat
      661 cctcgctgcg ccatcggcag gtgccgggat cacctcgacc cggtcgtggc cgatctgtga
      721 gatccgccag gcagtgctgc cgagcaggaa tacgtccccc tcacgcgatt cgtagaccat
      781 ctcctcgtcg agctcaccca cccggctccc gtccgggaga gtgacccgaa acagaccgcg
      841 gtccgggatg gtgccggcat tggcgacggc gagctggagg gcacctggcc gagcgctcac
      901 ctcgcctgtg gctcgatccc agttgattcg gggacgcaac tctgcgaaga ggtccgaggg
      961 atagcgcccg gcaagcatgt ccagcgtctc gtcgaacacg gcgcggctca gccctgaata
     1021 gggcgctgcc ctgatcgtca tctcaaagag ggcgtcggcg ctggacgccc cggctaccac
     1081 ccgggccacg agctgctgtg cgaggacatc gagcggattg gccgggatcc gggtggtctc
     1141 cacccttcgc tccagcattc gattggccac gacggccgat accaggagat ctccccggta
     1201 tttggggtag atgcgcgctt tggaggttcc ccccacctga tggcccgccc ggccgaccct
     1261 ctgcagaccg gaggcgaccg aggtcggggc ctccacctgg aggaccaggt cgaccgcccc
     1321 catatcgatg ccgagctcga gcgaggatgt cgcgaccacg gccttgagat cgccacgctt
     1381 cagcgcttcc tccgtgagga gcctctgctc gcgtgccacc gatccatggt gggccttggc
     1441 cacttcctcg ccggccagat tgttgatctc cgagcacagg cgctcggcca gccggcgcga
     1501 atttgcgaac acgatggtgc tgcgatgggc ttggatctgc tccagtagcc gggggtagac
     1561 cgcgggccag atggagcggg tcgactgctg gtccggatcg agagggtcgg gctctcctgg
     1621 ctcgttcatg tcctcgacgg gcaccacgat ctggatgtcc aggtcgcggt catgtgggac
     1681 atcgatcagg gtgacaggcc tcggctccca gataccgtcg tctccgatgg tcccgccgcc
     1741 aagatatctc ccgatgactt cgagagggcg ttgtgtcgcg gagaggccga ttcgctgcgg
     1801 cgagacgtcg gtgacctcct ccagtcgctc cagggagagg gacaggtgtg ccccccgttt
     1861 gctgcccgct actgcgtgca cctcgtcgac gatcacccag cgcacgctgc gcaagacctc
     1921 gcgagccgcc gaggtcagca tcaaatagag cgattccggg gtcgttatca gaatgtccgg
     1981 cgggttgcgg cgcatgcgct gtctcgcatc gggtggagtg tcaccggtgc ggatgaaggt
     2041 gtcgacggtg ggcagcgtcg ggccgccgat gcgactcgag gcgtggcgaa tgccggcgag
     2101 cggcgcccgg agattgcgct ccacgtcatg agcgagggcc ttgagaggtg agatgtagag
     2161 gacgcggcac cggcttgcct tgtcgggctg gggctcggtg agcagccgat cgagggtcca
     2221 cagaaacgcg gcgagcgtct tgcccgaccc ggtgggggcg tgaatcagag tgtggtggcc
     2281 atcggcgatg gcaggccatc cgagggcttg agcttgggtc ggctcgttga acgacgcctc
     2341 gaaccaggtg cgggttgctg gggtgaaggc gccgagcggg tccacacccg gaaggtatca
     2401 gaggtctagg acaacaacgg tcctggttcg ctgccccagg ccggggtcaa gcagagagct
     2461 cgagcggcag ccggttgatg gcggccagct cgaggaagag atcgaccacg tgtgggtcga
     2521 actggcttcc ggcgtgcttg cggatctcgg tgaccgcgaa ttcgacgggc agagcacgct
     2581 gataggcccg cgtcgaggtc atcgcatcga aggcgtcggc gaccaggatg atccggctca
     2641 ggatcgggat ctcctcggcg gcgaggccga acgggtaacc gcggccgtca tagcgctcgt
     2701 ggtggtagag gatcgcctct gcaacctttg ggtcgagacg gttgcgggtc atggcaaaac
     2761 cgagctcggg gtgacggttc atcagctcgc gctcggtcgc gtcaagaggc ccgggcttgc
     2821 cgaggatgcc cggatcgagg tggatcttgc ccacgtcgtg cagcacaccg gccagggcga
     2881 ggcggtcgag atcgatctct cccagcccga tgccgatacc gaggttcaca gccagatctg
     2941 agacccggtg cacgtgaccg tgcaggtcct cgatcacgtc ctccatgacc tcgatcaata
     3001 tgtctcttgg aagttcccgc attaagaaat cacccgacgg ggaagtctgc ttggagggcg
     3061 ctccgtgggg tatgggccga atggactata tgccgtcgac catgtggact agcccacagt
     3121 cgtctgctcg gaaattggag ccgaacgcca ggtttggcta actttccagc aagcgaaaga
     3181 cgcgctcatc aagagcggtg gagggacagg ccctacgaaa ccgcggcaac cgactcgaac
     3241 ggtgccaatg cctgaacgat gagtggccga ggacggctat ccgtcacggt gctcggaaga
     3301 gcgcgtagag acaggagaac cgtcatgaga gatcgagact tcgggttcga cacccgtcag
     3361 atacacgccg ggcagcggcc cgaccccgag accggtgccc gagcgatgcc catctatgcg
     3421 acgacctcat tcgtgttcga ggatgcccaa caagctgccg acctcttctc cttgcagaat
     3481 ttcgggaaca tctacacccg gatcggaaac ccgaccaatg ccgctttcga ggagcggatg
     3541 gcgtcgctgg aagggggcct gggtgccctg gccacgtcgt cgggtcaggc ggcacagctg
     3601 atctcgatct tcacgttgtg caacgcaggc gacgagatcg tggcctcccg atcgctgtat
     3661 gggggcacct tcacccagtt cgacgtgacg ctgcgccgga tggggatcaa cgtgaccttt
     3721 gtcgacatcc acgaccatgg agcggtcaaa gaggcgataa ctcccaagac caaggccctg
     3781 tatggggaga ccatcggcaa cccggcggcc gacatcctcg acatcagcgc catcgccgac
     3841 atcgcccacg acagcggcat cccgctcatc gtggacaaca ccttcgccac gccctatctg
     3901 tgccggccca tcgagcacgg ggccgacatc gtcgtccact ccgccaccaa attcatcggg
     3961 ggacatgggg tggtgatcgg cggggtgatc gtcgagtccg ggctgttccc tttcgacaac
     4021 gggaatttcc ccggcatcac cgagccctcg cctgcgtatc acgacctcat gttctgggag
     4081 aacttccggg agtacgccta tctgatgaag gctcgagtgg agtcgttgcg cgacattggc
     4141 gccgccatgt ccccgttcaa cgctttcatc atgctgctcg ggctggagac gctttcgctc
     4201 cgcatgtcgc gtcacaccca aaatgcgcgg agagtcgccg agttcctctt cgagcatccg
     4261 gacgtggcct gggtccgata cccgatattc cccaacaacc ggtggaccga atcggcgcag
     4321 aaatacgttc ccgacggccc gggagctgtg ttcaccttcg gggtgaaagg cggcttcgat
     4381 caggcggcca acttcatcgc cggggtcgag ctggcctccc atttggcgaa tgtcggggat
     4441 gccaagacgc tcgtgatcca tcctgccagc accacccacc agcaggtgcc tgtggaggag
     4501 cggcgggcag ccggtgtcgg tgacgagatg atccggatct cggtgggcct ggaggacatc
     4561 gacgacatcc tgtgggacct cgatcaggcc ctggaggggg cgcgcgaatg acgacggtca
     4621 tgcatgaagc gagcgcgctc gagcgtctgc agatcatcca gtcggcgagg agcgtggcgc
     4681 tggtgggcgt gtccgcgaac atcctgcgtt cgtccaactt cgtggccacc tatctggtcc
     4741 gcaccgacct cgacatgtac ccggtgaacc cgatgtacga cgagatcctg gggttgaaga
     4801 gctacccgtc tctggcggac ctgcctgttg tccccgacat cgtcgacgtg ttccgtcgcc
     4861 acgacgagct gcccaacgtg gtcgacgaag cgatcgccgt cggcgccaag gtggtctggt
     4921 tccagctggg gctgcgtcac gacgaagctg ccgccaaggc cgccgcagcc gggctgcagg
     4981 tggtacagga ccgctgtctc aagatcgagc acgcccgctt ctccgggaag ctgcatctgg
     5041 ccggcttcaa caccggtgtg atctcgtcac ggcgcctgag gccgctctag gtcagaccag
     5101 gccgtggtgg cgggccgcct cgatgaacgg ggcgacccgg gccatgtcga gcggggagtt
     5161 cgcggtaccg tcggctttga gcgacgagcc cacgatgact gtgtcggcga cttccacgag
     5221 taggccgagg ttctcgacag cggcgcccga cccgatgacc agcctcgtgc cctttgggat
     5281 cacggaccga gccatcgccg cctcgtgcag gtctggctcc agcccggtgc ctgcgccgga
     5341 gacgatcaca gcatcggcga ggccacggat caccgtgtcc tcagccgcct gcctgatgtc
     5401 gaggccaggc ggtggcgtgg cgtgcttgac catcacatcg gcccagatct cgatctccgg
     5461 ggccagagca gcccggcgac gggccaattc gtcggcgcgc cccacgatcg gcccttgatc
     5521 ggtgtacatg accccggtca gcacgttcac gcggatgaac gctgccccgg tgacgacggc
     5581 gatgccgaga gcggaaaggg cgtcgtttcg caagacattg acgccgaagg gaaccccggt
     5641 ggcatcactc acagctcgcg cggccagact catcgccgcg accgtttccg ggtcagaggc
     5701 ctctgcgtga aaggggacgt ctccgaagtt ctcgacgagg agggccggga aaccggcgtc
     5761 t
//
LOCUS       DASTXO010000162         3579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1225929, whole genome shotgun sequence.
ACCESSION   DASTXO010000162 DASTXO010000000
VERSION     DASTXO010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225929"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VFU96_03485"
     CDS             <1..895
                     /locus_tag="VFU96_03485"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5112352.1"
                     /translation="VVHTERRMSTSSSASVGFVGLGRMGSRIAGNLAKTGHEVTVWNR
                     TVSVAEDWAGENGGVVAGSLAELAQSEFIITMLADGEALEAVYGELAGRLGPEIVGID
                     MGTSGPESFEKVRGLVEARGATMVDAPVSGATAAAEAASLLVMVGAPDDVYERVEPVL
                     QSVGNPVQVGPTGSAAVLKLAVNSVLYGLNQAVAEAVALAEKSGVAPDTTIDILAKGA
                     AGAPMITYRREQYLHPDQAPITFTIDLARKDLMLALEHARRTGAPTGQLERTMELMED
                     LIERGFGSQDMGYVVEANRQA"
     gene            complement(915..2402)
                     /locus_tag="VFU96_03490"
     CDS             complement(915..2402)
                     /locus_tag="VFU96_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012595436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEU5112353.1"
                     /translation="MTLSLVERSGAWPERIAISDQDGDHTYSDLLYGSERGAACLLDA
                     NADLDEARIAFMVEPSFEYVRIQWAIWRAGGVAVPLCLTHPAPELEHVLDTTSPVIAI
                     ASPAYADLLQLLAEARGIEFLLVDEVDAEPVPMPRVDADRRAMILFTSGTTGRPKGVV
                     TTHANIEAQITSLVEAWEWEPDDRILLTLPLHHIHGIVNVIGCALWSGARCDILPSFD
                     AGAVIDRLATGDLTLYMAVPTIYHRLIAHLDQAADMDNAQFREGIGGLRLMVSGSAAL
                     PIPVLERWRELSGHTLLERYGMTEIGMGLSNPYRGERIPGSVGMPLPGVEVMLVDDEG
                     SEVSEGVPGEIRVRGPTVFLEYWDRPEDTDAAFTSDGWFRTGDTAVIEDGRYRILGRS
                     SVDILKTGGEKVSALEIEDVLLGHEAISEVAVVGLDDPEWGQRVVAVLVASRPVDGEE
                     LRRWAKGQLAPHKVPKEFHFTDSLPRNPMGKTQKPQVVQMLGEKG"
     gene            complement(2402..2782)
                     /locus_tag="VFU96_03495"
     CDS             complement(2402..2782)
                     /locus_tag="VFU96_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020133652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5112354.1"
                     /translation="MPRLDAIGVIVSDMARAIDFYRLVGLEFEGDDIHAEAAGPGGLR
                     VMLDGEANVMSFSDWQPPTGGSPRTALAFLCDSAADVDRLYEDLVEAGGHGHLPPFDA
                     PWRQRYASVHDHDGNAVDLFAPLE"
     gene            complement(2803..>3579)
                     /locus_tag="VFU96_03500"
     CDS             complement(2803..>3579)
                     /locus_tag="VFU96_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112355.1"
                     /translation="QVIDQGAPVYFDWAPDGTELLARVSGRFEYLAIDGSGRETVPVT
                     GEFRLGAHVGENVVLGTGRDVGEALALANRRGEIERELLRYAAPMAFVADETGSRLAV
                     MSRGSPESQELSRLEETDLPIIDAERLVVVETSDGDIEEVSPARNIAWFWSPDGQQLL
                     YSTVGAVDGIERLQWHIWDGEESTSYRPFSPTGLFGRDYLAYFDQFALSMSLWAPDGS
                     AFAYAGGSSLEDAGIWVQKVEGGDPLRVSSGEVALWSPAA"
BASE COUNT          618 a         1194 c         1185 g          582 t
ORIGIN      
        1 ggtcgtgcat actgagcgac gcatgtccac ctcatcttcc gccagcgtcg ggttcgtggg
       61 tctgggtcgg atgggctcga ggatcgcggg caacctggcc aagaccgggc acgaggtcac
      121 tgtgtggaac cgcaccgtct cggtggccga ggactgggcg ggggagaatg gtggcgtcgt
      181 cgccggcagc cttgccgagc tggcgcaatc cgagttcatc atcacgatgc tggccgacgg
      241 cgaagccctc gaagccgtgt atggggagct cgcgggcaga ctcgggccgg agatagttgg
      301 catcgatatg ggcacatctg gcccggagag cttcgagaag gtccgggggt tggtcgaggc
      361 ccggggggcg accatggtgg acgctcccgt ctcgggagcg accgcggccg cggaggcggc
      421 gtcgctgctg gtgatggtgg gcgctcccga cgatgtctat gagcgggtcg agccggtcct
      481 ccaatcggtg ggcaatcccg tccaggtcgg gcccaccggc tccgccgcgg ttctcaagct
      541 ggcggtcaac tccgtgctct acgggctcaa ccaggcggta gccgaggcgg tggcgctggc
      601 tgagaagtcg ggtgtcgccc ccgacaccac gatcgacatc ctggccaagg gcgccgccgg
      661 ggccccgatg atcacctatc gccgggagca gtatctccat cccgaccagg cgccgatcac
      721 gttcacgatc gatctcgccc gcaaggacct gatgctcgcg ctggaacacg cccggcgcac
      781 cggcgcgccc accggtcagc tggagcgaac gatggagctg atggaggacc tgatcgaacg
      841 cggcttcgga tcccaggaca tgggttatgt ggtggaggcc aatcgtcagg cttgaggcgc
      901 ggcgagcggc ctcttcagcc cttctctccg agcatctgca cgacctgagg tttctgcgtc
      961 ttccccatcg ggttccgggg cagcgaatcg gtgaaatgga actccttggg caccttgtgc
     1021 ggggccagct gccctttcgc ccaccgtctc agctcttcac cgtccacggg cctcgaggcg
     1081 accaggaccg ccaccactcg ctgaccccat tccgggtcgt cgagcccgac caccgccact
     1141 tcgctgatgg cttcatgccc aagaagcacg tcctcgatct cgagagccga caccttctcg
     1201 cccccggtct tgaggatgtc gaccgaagac cggcccagga tccggtagcg gccgtcctcg
     1261 atcactgcgg tgtcgccggt gcggaaccaa ccgtcggagg tgaacgcggc gtcggtgtcc
     1321 tcgggccgat cccaatactc caggaagacc gtcggccctc tgacccgaat ctcaccgggc
     1381 acgccctccg acacctccga cccttcgtca tcgacaagca tgacctcgac tcccggcaac
     1441 ggcatgccaa ccgagcccgg gatgcgctcc ccgcgatagg ggttggaaag gcccatcccg
     1501 atctcggtca tcccgtatcg ctcgagcaag gtgtgaccgg acagctcgcg ccatcgctcc
     1561 agcaccggga tggggagggc ggccgagccc gagaccatga gacggaggcc accgattcct
     1621 tcccggaatt gggcgttgtc catatcagcc gcctggtcga gatgggcgat cagccggtga
     1681 tagatggtgg gcacggccat gtaaagggtc agatcgccag tggcgaggcg atcgatgacc
     1741 gctccggcat cgaacgaggg gaggatgtcg caacgggctc cactccacag ggcgcagccg
     1801 atcacattga cgatcccgtg gatgtggtgc aggggaaggg tgagcaggat gcggtcgtcc
     1861 ggttcccatt cccacgcctc gaccagcgag gtgatctggg cctcgatgtt ggcgtgggtg
     1921 gtgaccacgc ccttcggccg gccggtggtc ccgctggtga agaggatcat ggcccgacga
     1981 tcggcatcga cccggggcat tggcaccggc tctgcgtcca cttcgtcgac gaggagaaac
     2041 tcgatccctc ttgcctccgc cagcagttga agtagatcgg catatgcggg cgaggcgatg
     2101 gcgatcaccg gactcgtggt gtcgagcacg tgctccagtt ccggtgcggg atgggtgagg
     2161 cagagtggga cagccacacc gcccgcccgc cagatcgccc actggatccg gacatactcg
     2221 aacgacggct cgaccatgaa cgcgatccgg gcctcgtcca gatctgcatt cgcatcgagg
     2281 agacaggccg cgccccgctc cgacccgtag agcaggtccg agtaggtgtg gtcgccgtcc
     2341 tggtcagaga tcgcgatccg ctccggccag gccccggatc gctcgaccag ggagagggtc
     2401 atcactccag aggggcgaac agatcgacgg cgttgccgtc gtgatcgtga accgaggcgt
     2461 agcgctggcg ccacggagcg tcgaatggag gcagatggcc gtggccgccc gcctccacca
     2521 aatcctcgta cagtcgatcg acgtcggccg cgctgtcgca gaggaaggcg agcgccgtgc
     2581 gcgggctacc tccggtgggc ggctgccagt cggagaacga catgacgttg gcctcgccgt
     2641 cgagcatgac ccgcagcccg ccgggtcctg ccgcctcggc atggatgtcg tcgccctcga
     2701 attcgagacc caccaaccga tagaaatcga tggcccgggc catgtcggag acgatcacgc
     2761 cgatggcgtc gagtcgtggc atgttcgcag tctatggcgg gatcaggcgg cgggtgacca
     2821 gagcgcgacc tctcccgaag agacgcgaag cgggtcgccc ccttcgacct tctgcaccca
     2881 gatcccggca tcctcgaggc tcgagcctcc ggcgtaggcg aaggcagagc cgtccggggc
     2941 ccagagcgac atgctgaggg cgaactggtc gaaataggcg agatagtccc ggccaaagag
     3001 cccggtgggg ctgaacggcc gatatgacgt cgactcctca ccgtcccaga tgtgccattg
     3061 caagcgctcg atcccatcga cggccccgac ggtgctgtag agcagctgct gaccgtccgg
     3121 gctccagaac caagcgatgt tgcgtgccgg gctcacctct tcaatgtctc catctgaggt
     3181 ctcgaccacg accaacctct ccgcgtcgat gatgggcaga tcggtctctt cgagccggga
     3241 gagctcctga ctctccgggg accccctgga catcactgcc agtcgactgc cggtctcatc
     3301 ggcgacaaac gccatcggag cggcgtaacg caacaactcg cgctcgatct cgccccgtcg
     3361 gttggccagg gccagtgcct cacctacgtc ccggccggtt ccgagcacca cgttctcccc
     3421 gacgtgggca ccgagcctga attcccccgt gaccgggacc gtctcacgtc ccgagccgtc
     3481 gatggccaga tactcgaacc gccccgagac ccgggcgagc agctcggtgc cgtccggcgc
     3541 ccaatcgaag tagaccgggg cgccctgatc gatcacctg
//
LOCUS       DASTXO010000163         4650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_231030, whole genome shotgun sequence.
ACCESSION   DASTXO010000163 DASTXO010000000
VERSION     DASTXO010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231030"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VFU96_03505"
     CDS             complement(<1..873)
                     /locus_tag="VFU96_03505"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEU5112356.1"
                     /translation="MAAPVLTTRTLNRSLLARQLLLERSALPITRAVERIGGIQAQYA
                     PSAYIGLWSRLQGFRRPRLTRALENAELIQATLMRVTIHVVSAADYAILTEAVRRSRR
                     ELWSRVARSRDLDQDGWERVTDIVTKALADGPRQRTDLIQVLIDEGFAKDIWEGVGVW
                     VDMVRVPPSGTWERRRADLYGLASDYVTMSPIEEHAALEHLIGRYLRAFGPATVGDIS
                     SWSGVSITALRQVIEGMRVREFRSEVGEVLLDLPRQPIPEPGKPAPVRFLPTWDATLL
                     AHARRTQILPEEHRS"
     gene            1091..1642
                     /locus_tag="VFU96_03510"
     CDS             1091..1642
                     /locus_tag="VFU96_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019073337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5112357.1"
                     /translation="MSSEGERRDRFETVVADVYDPLQRYIRRRAAADEAGDVLSDVLL
                     TMWRRLDDVPDGAALPWSYGVARRALSNHRRAASRHLRLVERLESEPPPAADDDPAGQ
                     LEDPELAAALAALPIADQEILRLWAWEQLEPREIAMALGSTANAVSLRLSRARKKLAD
                     ALARQDPSTAGHLGDETAEENRR"
     gene            1639..2247
                     /locus_tag="VFU96_03515"
     CDS             1639..2247
                     /locus_tag="VFU96_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112358.1"
                     /translation="MTDQLRDRLARLDPMHPGVETEPVTTESSQRLLEAIVSTPVIEQ
                     PETGPEQKPNRWLIGVAAAAALVLAVGGAIALTGDGEDPVASGPPLELSGGTADPSMT
                     MCIMYSVEELAKAPVAFEGTVTSVEGETVTLDVDHWFKGGDAEQVVINAPAGMRALLA
                     GIPFEVGTQYLVTAYDGTVNYCAFSGVSTPEFRADFEEAFGA"
     gene            complement(2490..2675)
                     /locus_tag="VFU96_03520"
     CDS             complement(2490..2675)
                     /locus_tag="VFU96_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112359.1"
                     /translation="MEEQERELITALYPALRKIAAVAGSVDIEPDDLVQEALMRVLRK
                     GSISDLDIRWLTFGRRS"
     gene            complement(2788..3711)
                     /locus_tag="VFU96_03525"
     CDS             complement(2788..3711)
                     /locus_tag="VFU96_03525"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEU5112360.1"
                     /translation="MDRYAPLPTVEQLGALGEGLGERIRFHQRIVGGLGGTVDVLVVG
                     AGAGERVVLKRYWLAEPGEISPAESEFRALALAIEHGISAPVPIWIDRIGLFPERAIV
                     ISHVEGAVLLDPEDPIDWAVQLADALVAIHRIQPEPTDQDLFPVLAATDGHQTEAETL
                     AALSGHPLGMDLWSRRTEALTSLEPERPVYVHHDFWPGNTLWVEEKLAAVVDWEGGAI
                     ADPALDVAYCALDMRLLGLKQAADSFVQVYRDRSGRALPNLPYWELLALCRPMPDIEM
                     WVPGWQAMGYEISTDEARLRHTALIAAALES"
     gene            3911..>4650
                     /locus_tag="VFU96_03530"
     CDS             3911..>4650
                     /locus_tag="VFU96_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEU5112361.1"
                     /translation="MNKTAEIYDTTLRDGTQAEGLSPTVGEKLRVVRLLDELGVHFIE
                     GGWPGANPKDDEFFARATKELDLERAQLVAFGSTRRPRGVVERDPQLQALLDADTDFI
                     CIVGKSWDRHVTDALNTSLEEGLDMVGESIDFLRQRGKRVFFDAEHFFDGFRNDPAYA
                     LSVVETAAGAGAERVVLCDTNGGALPSDIAPAVEAVSGLVETRLGVHFHNDSDCAVAN
                     SLTALSLGVHQVQGCINGYGERTGNADL"
BASE COUNT          838 a         1495 c         1491 g          826 t
ORIGIN      
        1 gctgcgatgc tcctcgggaa ggatctgggt gcgccgggcg tgggcgagga gagtggcgtc
       61 ccaggtgggg aggaatcgaa cgggggccgg tttcccgggc tcgggaatcg gttgccgggg
      121 gaggtcgagc aacacctccc cgacttcgga gcgaaactcg cggacacgca tcccttcgat
      181 tacctggcgc agggccgtga tcgagacccc gctccagctc gagatgtctc cgacggtggc
      241 cggcccgaac gcgcgcagat agcggccgat cagatgttcg agggccgcgt gctcctcgat
      301 cgggctcatc gtcacgtagt cgctcgccaa tccatacagg tcggcgcgac gccgttccca
      361 ggtgcctgaa gggggaaccc ggaccatatc gacccagacg ccaactcctt cccagatgtc
      421 cttggcgaac ccctcgtcga tgagcacctg gatcaggtct gtcctctggc gtggcccgtc
      481 ggccagggct ttggtgacga tgtctgtgac tctttcccag ccgtcctgat ccaagtcacg
      541 gcttcgtgcc acccggctcc acaattcccg ccgagaccgg cgaaccgcct cggtgaggat
      601 cgcgtagtcc gccgccgaga cgacatgaat tgtcactcgc atcagcgtcg cctggatcag
      661 ctcggcgttc tccagggccc gggtcagtcg tggtcgccga aacccctgga gacgagacca
      721 gagccctatg tacgccgagg gcgcgtactg ggcttgtatg ccgccaatcc tctccacggc
      781 tcgcgtgatc gggagcgccg acctttcgag aagcaattgg cgggcgagca gggagcggtt
      841 gagagttcgc gtggtgagga cgggcgcagc catcgacggt cagggtaagt cccgaaagca
      901 ggcgaaccag gtgcggggtg gtggcaccgc acctggctct cagggaggat cccgaccggg
      961 gggagccaat cggatccggc atttggcttg ctgctgaacg atgatttcgt caggacgacc
     1021 aagaccctgg atgggtccgc ggacccatga acggtgacag aatccggcct gtgccggaca
     1081 ctggctgttg gtgagctccg aaggtgagcg gcgggaccgc ttcgaaacgg tcgttgccga
     1141 cgtgtatgac ccgctgcagc gatatatacg tcgccgcgcc gcagctgatg aggctggcga
     1201 cgtgctctcc gacgttctgc tcaccatgtg gcgtcgtctc gacgatgtgc ccgacggtgc
     1261 ggcccttccc tggtcttacg gtgtggcccg acgagctctg tccaatcatc gacgcgccgc
     1321 ctcgcggcat cttcggctgg tggagcgtct cgagtccgag ccgccgcccg ccgccgacga
     1381 cgatccggcc gggcaactcg aagaccccga gctcgccgcg gcgctggcgg ccttgccgat
     1441 tgctgatcag gagatccttc ggctctgggc gtgggagcag ttggagccca gagagatcgc
     1501 gatggcactg ggcagcaccg cgaatgcggt gagtctccgt ctcagtcgag cccgcaagaa
     1561 actggccgat gcattggccc gacaggatcc ttccactgcc ggacacttag gggatgagac
     1621 cgccgaggag aatcgacgat gacagaccag ctacgtgacc ggcttgcccg tctcgatccg
     1681 atgcacccag gcgtcgaaac agaacccgtg acgaccgagt cgtcccaacg ccttttggag
     1741 gccatcgtga gcacaccagt aatcgagcaa cccgagaccg ggccggagca gaagcccaac
     1801 cgatggctga tcggagtcgc cgccgcagcc gctctcgtcc tcgccgttgg gggggcgatc
     1861 gccctcaccg gtgacgggga ggacccggtt gcatcgggcc ccccgctcga gctcagcggt
     1921 ggcaccgctg acccgtcgat gacgatgtgc atcatgtaca gcgtcgagga gctggccaaa
     1981 gctccggtcg ccttcgaggg caccgtgacc tcggtcgagg gcgagacggt caccctcgac
     2041 gtcgaccact ggttcaaggg tggcgatgcg gagcaggtgg tcatcaatgc tccagcgggg
     2101 atgcgggcgc tgctcgccgg catcccgttc gaggtcggta cccaatatct ggtcacggct
     2161 tacgacggga ccgtcaacta ctgcgcattc agcggtgtga gcacccccga gttccgcgca
     2221 gacttcgagg aggccttcgg agcctgaaca cctgctcgac ggtgaagggg agaggccgat
     2281 gagggcctct cccctttccg cgcccgggcg cctcggcata ggcataccgt ccatctcgac
     2341 ggggtagagg attgccctgt gtcttggagg aagatcgagg atcgcctcga tatcggcctg
     2401 atagctgggc aaccagctct cctcgccaga aagccgagct acggccttcc gcagtcgacc
     2461 gaggctgcgc cgctggttcg aggccaggtt tacgatcgtc ttccgaaggt aagccagcgg
     2521 atgtcgaggt cggagatgct ccctttcctc aacactctca tcagcgcctc ctgaaccaga
     2581 tcgtccggct cgatgtcaac ggagcccgcc accgcagcga tctttcgcag agccgggtag
     2641 agagccgtta tcaactccct ctcctgctct tccaaagaag atcgccttgg caatcgaagt
     2701 ttctccctgt cgtcacgcat taggacaccg cggcatgccg atgtgtgaca caagattcct
     2761 agcggtcatg tcacgcccat tggagattca cgattcgaga gcggctgcga tcagcgcagt
     2821 gtgtcggagt cgagcctcgt cggtgctgat ctcataaccc atcgcctgcc atcccggcac
     2881 ccacatttcg atgtcgggca tcgggcgaca tagtgcgagc agctcccaat agggcagatt
     2941 cggtagggct cgccccgagc gatcccggta gacctggacg aaactatccg ccgcctgttt
     3001 cagacccagc agccgcatgt cgagcgcgca gtaggcgaca tcgagggcag ggtcggcgat
     3061 ggcgccccct tcccaatcga ccacggcggc gagcttctcc tcgacccaca atgtgttgcc
     3121 gggccagaaa tcgtggtgta cgtaaaccgg gcgttccggc tccagcgagg tcagcgcctc
     3181 ggtgcgtctc gaccagagat ccatgcccaa tgggtgtccg ctcaaggcag cgagagtctc
     3241 cgcctcggtt tggtggccgt cggtagcggc cagtaccggg aagaggtcct gatcggtggg
     3301 ctcgggttgg atccgatgga tggccaccag tgcatccgcc aattgcacgg cccagtcgat
     3361 gggatcttcc ggatcgagaa gcacggcccc ttcgacatgc gagatgacga tcgcccgctc
     3421 cgggaacaac ccgatccggt cgatccagat cggaacaggt gcgctgatcc cgtgctcaat
     3481 cgccagggcg agggcacgga attcgctttc ggccgggctg atctccccgg gctcggccag
     3541 ccaatagcgt ttcagcacaa cgcgttcgcc ggcaccagcc ccaacgacga gcacatccac
     3601 tgtcccaccg agaccgccga cgatccgctg gtgaaaccga atcctttctc cgagcccttc
     3661 tcccagcgcg ccaagctgtt cgacggtcgg caacggcgca tatcggtcca tgatcctcag
     3721 ggtattgcga ggcaattcga cccgtcttga ctaagccggt ctccgttccc atactgagca
     3781 accgtatgag aggaatgatc gagccgcgca cgcccatcat cattctctag gggcccgcgc
     3841 cttcgaggcg ccgcccctgc accggacctc cgccaactgc gggggtcctt tctattgcca
     3901 gggacaaatc atgaacaaga cagccgagat ttacgacacg acccttcggg atggcactca
     3961 ggccgagggc ctctcgccga cggtaggaga gaagctgcgg gtggtgcggc tgctcgacga
     4021 gttgggagtc cacttcatcg aagggggatg gcccggggcg aaccccaagg acgacgagtt
     4081 cttcgcccgt gcgacgaagg agctcgacct ggagcgggca cagctggtcg cattcgggtc
     4141 tacgcgccga ccacgcggtg tggtggagcg cgatccccag ctccaagcgc tgctcgatgc
     4201 agacacggat ttcatctgca ttgtcggcaa gtcatgggat cgccacgtga ccgatgccct
     4261 caacacttcg ctggaggagg gcctcgacat ggttggcgag tcgatcgact tcctgcgaca
     4321 gaggggcaag cgggtcttct tcgatgccga gcacttcttc gacggctttc gtaacgaccc
     4381 ggcttacgcc ctatcggtgg tggagacggc ggctggcgcc ggtgcggagc gcgtcgtgct
     4441 atgcgacacc aacggcggcg cccttccctc cgacatcgcc cccgccgtcg aggccgtttc
     4501 cgggctggtg gagacccgat tgggagttca tttccacaac gactccgact gcgcggtggc
     4561 caactcgctg acggcactct ccttgggggt ccatcaagtg cagggctgca tcaacggata
     4621 cggagagcga accggcaatg ccgatctgtg
//
LOCUS       DASTXO010000164         2127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_10411, whole genome shotgun sequence.
ACCESSION   DASTXO010000164 DASTXO010000000
VERSION     DASTXO010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10411"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VFU96_03535"
     CDS             <1..622
                     /locus_tag="VFU96_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112362.1"
                     /translation="RVAAALKLHAPVIKPNAEYATSQTASGYPHRLEGLARAGATWND
                     ARNRQNSGECRMKTRFGRFVLFSLVCLGSSSFGARADDPGHAAVPNVVGTWTGEFKLM
                     HWTGPAEQALELQVLEQDGPLVKGEKRWRIKPGGTAGNVAGQDRREATEPLVGVIGFD
                     GAMYLAEQGDSGLYTGRLTGPDTLEVVYIEAGDLATAYRAVLKRTK"
     gene            complement(656..1336)
                     /locus_tag="VFU96_03540"
     CDS             complement(656..1336)
                     /locus_tag="VFU96_03540"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEU5112363.1"
                     /translation="MPGFVSGEPHHFPLRGTLRPETTALVVIDMQHDFLSEGGYMHRL
                     GADLSGLREVIEPIRAVLTRARAWGCRIVHTREGYAADLSDLQPWKLGGSPGDAIAIG
                     DKGPRGRALIRGEPGFDIIPELAPAPGEPVFDKPSYGAFATTTLEDHLRANGVTHLVL
                     TGVTTDCCVTSTLREALDLGFECLVLTDCVGSATRSHHEAALTLMRKPSGVFGTTTNS
                     QVFLEALP"
     gene            1493..>2127
                     /locus_tag="VFU96_03545"
     CDS             1493..>2127
                     /locus_tag="VFU96_03545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5112364.1"
                     /translation="MSRPDYPFNTSDHERQRLMRQANILADTTERLLRKAQIGPGMRV
                     LDIGSGAGDVAFLARRLVGESGEIIGTDRDGEQVSFANQRAASLGYGNVRFVQSDFAS
                     LTLDKPVDAISGRLVLLFAKDPTAAVAGVCRNLRGGGVVAFLESNFLFDAPVLVEPRN
                     SLAGKAVWWIAEGLKHAGVQPRLGLRLFSIMKDAGLEPSPQIESTLNVGQGP"
BASE COUNT          416 a          632 c          692 g          387 t
ORIGIN      
        1 ccgggtcgcg gcagcactca agcttcacgc gcccgtcatc aaaccgaacg cggaatatgc
       61 gacctctcaa accgcgtcgg gctatcctca ccggctcgaa ggactggctc gcgccggcgc
      121 gacgtggaat gatgctcgca atcgccaaaa ctctggagag tgccgtatga agacccggtt
      181 tgggaggttc gtgctctttt cgttggtttg tctcgggagt tcgtctttcg gggcccgagc
      241 cgacgacccc ggacatgctg ctgtcccgaa cgtcgtgggc acatggactg gcgagttcaa
      301 gctcatgcat tggacaggcc cagccgaaca ggccctggag ctgcaagtcc tcgagcagga
      361 tggtccgctg gtcaaaggcg aaaagcgatg gcggatcaag ccagggggta cagcggggaa
      421 tgtcgcggga caggatcgca gggaggcgac cgaaccactg gttggcgtga tcggctttga
      481 cggcgcaatg tacttggccg agcagggcga cagcggtctc tataccgggc gcctgaccgg
      541 tcccgatacc ctcgaggtcg tctatatcga ggctggagac ctggcgaccg cttatcgggc
      601 ggtgctgaaa cgaacgaagt agcggcacga cgccgtcgcc aaatatcttg ggtggctaag
      661 gaagggcctc gagaaatact tgcgaattgg tggtcgtccc gaacaccccg ctcggcttgc
      721 gcatcaaggt gagcgccgcc tcgtgatggg agcgcgtggc actgccgacg caatcagtga
      781 ggaccagaca ctcgaaaccg agatccaggg cctcgcgcag ggtcgaggtc acgcagcaat
      841 cggttgtcac ccccgtcagc acgaggtggg tgacgccatt ggcccttaga tggtcttcga
      901 gcgtggtcgt cgcgaaggcg ccatagctcg gcttgtcgaa gacgggctcg cccggtgccg
      961 gggcgagttc cggaatgatg tcgaatcccg gctcgccacg gatgagcgcc ctgccgcgcg
     1021 gccccttgtc gccaatggca atggcatcac ccggcgaacc gccaagcttc caaggctgga
     1081 gatcggagag gtcggcggca tagccctcgc gggtgtgcac gatccggcag ccccaggcgc
     1141 gggccctggt caagaccgcg cggatcggct cgataacctc tctcaagccg gagagatcgg
     1201 caccgagccg atgcatgtaa cccccttcgc tcaggaagtc gtgctgcatg tcgatcacca
     1261 cgagggcggt ggtctcgggt cgaagagtgc cgcgcagggg aaagtggtgc ggttctcctg
     1321 aaacgaatcc gggcatcgtc cttcggcctc ttcgtgtcac cagggcggtt cccgaggata
     1381 gtcgcagcac gaatcgtcgt tcaaccccca aggccgcggg ttctgcaatt gacccgaatg
     1441 tcgaaggaat gtgatcaatc atgctccgat caaagacacg agggaagagc ccatgtcacg
     1501 tcctgactac ccgttcaaca cttctgatca tgaaaggcag cgcctgatgc ggcaggccaa
     1561 tatcctggcg gacacaaccg agcgactcct gcgcaaggct caaatcggtc cgggcatgcg
     1621 ggtcctggat ataggcagcg gtgcgggaga cgttgccttt ctcgctcgca ggttggtcgg
     1681 tgaatccgga gagatcatcg gcactgacag ggacggtgaa caagtctcct ttgccaatca
     1741 aagagcagcg tccctcggct acggaaacgt tcgtttcgtc caatccgatt tcgcttccct
     1801 gacattggac aaaccggtcg acgcgatcag tggcaggctc gtcctcctct ttgccaagga
     1861 cccgacggcg gctgttgctg gtgtctgtcg caatcttcgc ggcggcggcg tcgtcgcatt
     1921 cctcgaaagc aactttctat tcgatgcacc ggtgctggtc gaaccgcgca acagtttggc
     1981 cgggaaggcc gtttggtgga tagccgaggg gctgaaacac gcaggcgtgc agccgcgcct
     2041 gggactgcgg ctgttcagca tcatgaaaga cgccgggctt gagccgagcc cgcagatcga
     2101 gtcgacgttg aacgtcggcc aggggcc
//
LOCUS       DASTXO010000165         4360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1483779, whole genome shotgun sequence.
ACCESSION   DASTXO010000165 DASTXO010000000
VERSION     DASTXO010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483779"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /locus_tag="VFU96_03550"
     CDS             complement(<1..1042)
                     /locus_tag="VFU96_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5112365.1"
                     /translation="MRRLRASSAPFLSIVAPPGYGKTTLLSQWAEASDRPVAWVSVDE
                     RDNDPSVLLAHVAAAIDRIEPLDPGFLRVVTSPRFGGALRAVHKLAATFSELSQPVSL
                     VLDQCEALSNQECFDLLAELSMSLGEDSRVAMASRTVPPLPIALMRSRGQLEEFGFDE
                     LAMDVNEAEHLYDKVGLRLTSDEVGDLVRRTEGWPVGLYLAALAFNAEGQGQAAVVSF
                     NGDDQFIADYLKSELLGRMSPQEVTFLTRTSVLEQMSGPLCDEVVGRDDSARLLEEME
                     RRNLLIVPLDRHREWYRYHHLLRHMLRRELERTEPDSIETLNLRAASWFERHGQPEVA
                     LKHAQAAGDADSV"
     gene            complement(1408..2424)
                     /locus_tag="VFU96_03555"
     CDS             complement(1408..2424)
                     /locus_tag="VFU96_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEU5112366.1"
                     /translation="MTTVVAAALLLAVIAYAVFGGADFGAGFWDLFAGGDKRGAEPRR
                     VIERAIGPVWEGNHVWLIFIFVVLWTGFSEAYASIWLTLFVPLTIAAFGIVLRGAGFA
                     FRKTVTTTRYQRIFGGAFAIPSLIVPYCMGAVVGAIASGRVPAGGIAGDPVGSWVNPT
                     SILGGILAVTVAAYLASVYLVVDAARVGQEGMVDYFRRRAVAMAVIAGLVAGAGIFVL
                     RDDAVYVFDGLTTRALPIVIASVLLGVVSLVMLVRRTHARLTRVSAALAVATIVLAWG
                     VAQWDFILPETLTLEQASAPEGTLGALIVAAVLVVVLVGPAFIWLYRLDQQDMLPEES
                     IENA"
     gene            complement(2421..3845)
                     /locus_tag="VFU96_03560"
     CDS             complement(2421..3845)
                     /locus_tag="VFU96_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020104784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEU5112367.1"
                     /translation="MEPVVAVDVVTWARAQMAFTLAFHIILVPLGVSWAAMTLIANYR
                     GLKHGEKDALRLAQRWSKYMAVTFAVGAVTGTVLSFEFGLLWPAFMDQWGEAFGIPFA
                     FEGIFFFTEAVFIAIYIFGWRRLKPWTHFWTGVPVVIAGIFGSISVVAANAWMQTPQG
                     FTLDASGDITAVDPIAVILNPSMPLQAAHMLFAAYMVGPFLVASVYAVALLRGRRDRY
                     HRLGFIIAFTVAAVATPIQMGVGDSLARWVYNNQPMKFAAIELVPETASDVPETILGR
                     LTDAWTVEGGIPIPGLASWLSDPSTGSATVIQGLDSFPQEDLPTVRQINIVHLSWDLM
                     VGIGTLLFLLTLWYWGSWIFKKDMPKSRLFFWIASAGGVLSIVAMEAGWIVTEVGRQP
                     WIVYELMRVEDAATLNEGVVVTFFTVLALYLAVGATTILVLRAMKRRFERREQGENGF
                     ADHDVPYGPSAPAAVASSSEKAEE"
     gene            3937..>4360
                     /locus_tag="VFU96_03565"
     CDS             3937..>4360
                     /locus_tag="VFU96_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HEU5112368.1"
                     /translation="MSPGFEECLFQGEPGIKLIAGETEATFLPAVGMTGVSLRHRGNE
                     YLDMPGGVEGLRERSTAGLPLLAPWANRLSSWEYTVGDTHVDLSGSKVPTRDNHDLPI
                     HGLLLASTDWQIDRQRTSRGKARLDASIHVDMPEFPFHH"
BASE COUNT          928 a         1363 c         1352 g          717 t
ORIGIN      
        1 ccactgaatc tgcgtcgcct gccgcctggg cgtgcttgag cgccacctcg ggctgaccgt
       61 gtcgttcgaa ccaagaggcg gctcgaaggt tgagggtttc gatcgagtcg ggctcggtgc
      121 gctctagctc cctccgcagc atgtggcgga gcagatggtg ataccggtac cactcgcggt
      181 gccggtcgag ggggacgatc aggagattgc ggcgctccat ctcctcgagt aggcgcgcgg
      241 agtcatcacg accaacgact tcatcgcaca acggaccgga catttgctcg agcaccgagg
      301 ttcgggtcag gaaggtcacc tcttgcggcg acattcggcc gagaagctcg gacttcaagt
      361 agtcagcaat gaactggtca tctccgttga agctgacaac ggccgcctgt ccctggcctt
      421 cggcgttgaa agccagagcg gcgaggtaga gaccgacagg ccatccctcg gtgcgtcgaa
      481 ccagatcccc cacctcatcg gatgtgagcc tgagaccgac cttgtcgtag agatgttcgg
      541 cttcgtttac atccatggcc agttcgtcga aaccgaattc ctccagctga ccgcgcgagc
      601 gcatcagggc gatcgggagc gggggaacgg tgcgggaggc cattgcgacc cgcgagtcct
      661 cgccgagact catcgacagc tcggcgagca agtcgaaaca ttcttggttg gaaagagcct
      721 cacattggtc gagcaccaag ctcactggct gagacaattc agagaaggtc gccgccaatt
      781 tgtgcactgc ccgaagagcc cctccgaagc gaggagaggt gactacccga aggaatccag
      841 gatccaatgg ctcgatgcga tcgatggcgg cggccacatg ggcgaggaga accgaagggt
      901 cgttgtcccg ctcgtccacc gacacccacg ccaccggccg gtcgctcgcc tcagcccact
      961 gtgacaggag agtcgtcttg ccataccccg ggggggccac tatcgacagg aaaggcgccg
     1021 acgaggcccg gagacgtctc acgaggaccc ttctctcgac gatctcggcc cgtgggatgg
     1081 gtggatggag tttcgaatcc gggatctcga aaggtggcca gacaagggtt cccacacctg
     1141 agccaaccct ggtctgagcg gctcgggctt ggcgttcatg cccgtcaggc accgcctgcc
     1201 gcgaccggtg cgacgcactc acagctgggc gactttcgaa gagaaggcac cgaagaagcc
     1261 gttggattct cgcgcctcga tcagggcggc gtgatcagac ctacccatgc ccacgtgacg
     1321 tcggcgttcg cacgccatcc gggcaagcta gttgtttcgg agagaacagg aatcagctag
     1381 ggcggggcca aggcaatttg ggttggctca ggcattttca atgctttcct cggggagcat
     1441 gtcctgctgg tcaaggcgat acagccagat gaatgccggg cctaccagga ccacgaccag
     1501 caccgcggcg acgatcagcg cgcccagcgt cccctctgga gcggatgcct gttcgagggt
     1561 caatgtctcg gggaggatga agtcccactg ggccactccc catgcgagaa cgatggttgc
     1621 gaccgccaga gcagcgctga ctcgggtgag tcgggcgtgg gtccgcctga ccagcatcac
     1681 gagcgagacg actcccagga gaaccgaggc gatcactatc gggagcgctc gtgtggtgag
     1741 accgtcgaag acgtagaccg catcatctcg gagcacgaag atccctgctc cagccaccag
     1801 accggcgatc actgccatgg caacggctct gcggcgaaag tagtcgacca tcccttcttg
     1861 gccgactcga gctgcgtcaa cgactagata gacgctcgcc agataggcag ccacggtgac
     1921 agccagaatc cccccgagaa tggatgtggg attgacccac gagcccacag gatcacctgc
     1981 aatcccaccg gccgggaccc gacccgaggc gatcgcgccg accacggccc ccatgcaata
     2041 gggcacgatg agcgacggaa tcgcgaaggc accgccgaag atgcgctgat aacgggttgt
     2101 ggtcaccgtt ttgcgaaaag cgaagccggc gcctcggagc acgatcccga acgcagcgat
     2161 ggtgagcggg acgaacaggg tgagccagat cgaggcataa gcctcggaga aaccggtcca
     2221 caggacgacg aagatgaaga tgagccatac gtggttgcct tcccagaccg ggccgatggc
     2281 tcgctcgatg actcgcctgg gctcggcgcc cctcttgtca cccccggcga agagatccca
     2341 gaaaccggcg ccgaagtcgg ccccaccgaa gaccgcatag gcaattactg cgaggagcag
     2401 agccgcggcc acgactgtgg tcattcctca gccttctctg acgacgaggc aaccgcggcg
     2461 ggagcggatg gcccgtaagg cacatcgtga tccgcgaagc cgttctcgcc ctgctctctc
     2521 cgctcgaagc ggcgcttcat cgccctgagg acgaggatgg tggtcgcgcc cactgccagg
     2581 taaagggcaa gcacggtgaa gaaggtcacc accactcctt cattgagcgt ggcggcgtct
     2641 tcgacccgca tcagctcata gacgatccat ggctggcgcc caacttccgt cacgatccat
     2701 ccggcttcca tcgcgactat cgacaggacc ccgcccgctg aggcgatcca gaagaacagc
     2761 ctgctcttgg gcatgtcctt cttgaaaatc caggaacccc aataccaaag ggtcagcagg
     2821 aacaggagcg ttccgatgcc caccatgagg tcccacgaca ggtggacgat gttgatctgc
     2881 cggaccgtcg gtaggtcctc ttgggggaat gagtcgaggc cctggatgac ggtggcggac
     2941 cccgtcgacg ggtcggatag ccatgatgca agaccgggaa tggggatgcc tccttccaca
     3001 gtccaagcgt cggtcaaccg ccccaggatc gtctcgggga cgtcgctcgc agtttccggg
     3061 accaactcga tagcggcgaa cttcatcggc tggttgttgt acacccagcg ggccagtgaa
     3121 tcgcccaccc ccatctgaat cggcgtagcg acggcggcaa cagtgaacgc gatgatgaaa
     3181 ccaagacggt gatagcggtc gcgccttccc cggagcaggg cgactgcata gaccgacgcg
     3241 accagaaaag ggccaaccat gtaggcggcg aacagcatgt gggctgcctg caacggcatc
     3301 gaagggttca agatcaccgc gatcggatcg actgcggtga tgtcccccga cgcgtcgagg
     3361 gtgaatccct gaggtgtctg catccacgca ttggcagcga cgaccgagat cgatccgaaa
     3421 atccccgcga tgacaaccgg aacacccgtc cagaagtgag tccaaggctt caaccgacgc
     3481 caaccgaaga tgtagatggc aatgaagacc gcctcggtga agaagaaaat cccctcgaag
     3541 gcgaagggaa tgccgaaagc ctccccccac tgatccatga acgcgggcca gagcaacccg
     3601 aactcgaagc tgagcaccgt gcctgtgacc gctccaacgg caaaggtgac cgccatgtac
     3661 ttcgaccacc tctgagcgag acgcaacgcg tccttctcgc catgtttcag gccccgatag
     3721 ttggcgatca gagtcatcgc cgcccaggac acacccagtg gcaccaggat gatgtgaaat
     3781 gcaagggtga acgccatctg ggcacgtgcc caggtgacca cgtcgaccgc aacgaccggt
     3841 tccacggctt acgagctttg cggattgcga ctgcgcgcgc atcatccggt ctggacgaaa
     3901 gccgcatccg gcgatcgcgg tagggtgaca cgagtcatga gccccggctt cgaggagtgt
     3961 cttttccaag gtgagcccgg gatcaagttg atcgctggag agacggaagc cactttccta
     4021 cccgcagttg gaatgaccgg cgtgtcgctt cgccatcggg gaaacgaata cttggacatg
     4081 ccgggcggtg tcgaagggct ccgggaacgg tcaactgccg gtttgccctt gttggcgccg
     4141 tgggccaatc gcctgtcgtc ctgggagtac acggtcggtg acacgcacgt cgacctgagt
     4201 ggcagcaagg tgccgacacg tgacaaccac gatctcccga tccacggcct cctcctcgcg
     4261 tccaccgatt ggcagatcga ccgtcagcgg accagtcgcg gaaaggccag gctcgatgcc
     4321 tcgatccacg tcgacatgcc ggagttccct ttccaccatc
//
LOCUS       DASTXO010000166         3131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_783924, whole genome shotgun sequence.
ACCESSION   DASTXO010000166 DASTXO010000000
VERSION     DASTXO010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783924"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1550
                     /locus_tag="VFU96_03570"
     CDS             <1..1550
                     /locus_tag="VFU96_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005254338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU5112369.1"
                     /translation="LAELLPDDAERIVGGEPETVPISELVVGDLVLVRPGARVPADGA
                     IVSGEAELDESMVTGESNPVGKRAGDRVVAGTVATDSAIRVEVTAVGDDTALAGIQRL
                     VAQAQQSRSRAQVLADRAAALLFYVAVGAAAITAIVWSALGQPEDAVTRTVSVLVIAC
                     PHALGLAIPLVISISTGLAASNGILVEDRLALERMRTVDVVLFDKTGTLTRGRHVVSD
                     VAAIDGGIDRVLALAAAVEADSEHPLAHAIVAEAAARGTVPATTEFRAMSGRGVEAKV
                     DGETVAVGGPALLRERGLELPPDLDTAVSSWEERGAAALFVIRGEKTIGVISLEDEVR
                     PESRPAVDELHQLGVRVAMITGDARQVAESVAAELGIDEVMAEVLPEDKHSRVAELQS
                     RGLTVAMVGDGVNDAPALAQADVGVAIGAGTDVAIESAGIVLAADDPRAVVAITRLSS
                     AGYRKMVQNLLWATGYNVVAIPLAAGAMAWAGVVLGPAASAILMSVSTIVVAINAQFL
                     RGMDLGG"
     gene            1614..>3131
                     /locus_tag="VFU96_03575"
     CDS             1614..>3131
                     /locus_tag="VFU96_03575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEU5112370.1"
                     /translation="MQTNANISSLVDGWTDQGRGDDTILITADDVAWSAGRLLQGVSA
                     VAGRLREIGVGREDRVLLVLDDTPAFPAAFLGAMRIGAVPVPVNFLARPDDFGYFLDD
                     SYASVAIIDSAFLDVVGPQVAARPWVRLVVANGTGPEGTDSFDAWIEETGEEIDPVRT
                     HPDDMAFWLYSSGSTGRPKGVVHRHADVAATCTHYAEPVLGIGPGDVVFSSTKMFHAY
                     GLGNNLTFPMWSGATSVYSIGRPTPDRLLERVRTHRPSLYFSVPALYNAILAHAEFDD
                     TDWSSVRLGISAAEPLPPEIWRRFHDRTGVEILDGVGSTEMLHIYCSNRAGAVRPGTS
                     GTPVDGYEMEVRDPEGSLCPVGEAGEMWVRGPSALTMYWHQHQRSQDKIRGAWFLSGD
                     RFRQDEDGYFWYEGRVDDMMKVGGLWVSPIEIENRLVEHPTVREAAVVAIDIDHLSRI
                     KAVVILAEGVTPGEDLDAELQAWCKSGLQRYQYPHVIEYVDDFPRTATGKIQRFRL"
BASE COUNT          494 a          974 c         1114 g          549 t
ORIGIN      
        1 cgctggcgga gttgctgccc gacgatgcgg agcggatcgt cggaggggaa ccggagaccg
       61 tgccgatctc ggagctcgtg gtgggcgacc tggttctcgt ccgtcccggg gcccgggtcc
      121 cggccgacgg ggcgatcgtc agcggtgagg cggagctgga cgagtcgatg gtgaccgggg
      181 agtcgaaccc ggttggcaag agggcgggtg accgagtggt cgccgggact gtcgccaccg
      241 attcggctat cagagtcgag gtcactgccg taggggacga caccgctttg gccgggatcc
      301 agagactcgt tgcccaggca caacagtcga ggtcccgggc ccaggtgctg gcggaccggg
      361 cggcggcgtt gctgttctat gtggcggtcg gagctgctgc gatcacggcg atcgtctggt
      421 cggccctggg gcaacccgag gacgcagtga cgcgcactgt gtctgtgctg gtgatcgcgt
      481 gccctcacgc cctcgggctc gccatacccc tggtcatctc gatctcgacc gggttggcgg
      541 cgagcaacgg gatcctcgtc gaggatcgcc ttgccctgga acggatgcga accgtcgacg
      601 tcgtgctttt cgacaagacg ggcactctga ctcggggccg tcacgtggtc agtgatgtgg
      661 ccgccatcga cggtggcatc gaccgagtat tggccctggc ggctgcggtg gaggccgact
      721 ccgagcatcc gttggcccac gccatcgttg ccgaagccgc cgcgcgtggc acggttcctg
      781 cgaccaccga gtttcgggcc atgagtgggc gcggcgtgga ggcgaaagtg gatggtgaga
      841 cagtggccgt gggcggaccg gcgctgctcc gggagagggg gctcgagctg ccccccgatc
      901 tcgatacggc ggtatccagc tgggaagaga ggggggccgc ggcgctcttt gtcatcagag
      961 gagagaaaac gatcggggtg atctccctcg aggacgaggt acgacccgag tcccgcccgg
     1021 ctgtcgacga gctccaccag ctgggagttc gggtggcgat gatcaccggc gacgcacgcc
     1081 aggtcgccga gtcggtggcg gcagagctgg gcatcgacga ggtcatggcc gaggttctgc
     1141 ccgaggacaa gcactccagg gtcgccgagc ttcagagccg gggtttgacg gttgcaatgg
     1201 tcggcgacgg tgtcaacgac gccccggccc tggcccaggc ggacgtcggc gtcgctatcg
     1261 gagcgggcac cgatgtcgcc atcgagtcgg ccgggatcgt gctcgccgcc gacgacccgc
     1321 gtgcagtcgt cgccatcacg cgtctctctt cggccggtta ccgcaagatg gtgcagaacc
     1381 tcctctgggc caccggttac aacgtggtgg cgatacccct ggccgccggc gccatggctt
     1441 gggccggagt ggtcctgggt cccgcggctt ccgcgatcct gatgagcgtc tccaccatcg
     1501 tcgtggcgat caacgcccag ttcctcaggg gtatggacct aggcggttga cgatcgcgtg
     1561 ccggcttcgg tcatcgaccg atagtctcgg tcggtcggga ccggcagggg cagatgcaga
     1621 ccaatgccaa catcagcagc ctggtcgacg gttggaccga tcaggggcgg ggcgacgaca
     1681 cgatcctgat cactgccgac gacgtggcct ggtcggccgg acgcctccta cagggggtgt
     1741 cggcggttgc cggccgtctc cgtgagatcg gggtgggacg ggaggatcgg gtcttgctcg
     1801 tcctagacga caccccggcc ttccccgctg ctttcctcgg ggcgatgcgg atcggggcgg
     1861 tgccggtccc ggtcaacttc ctcgcccggc cggacgactt tggctacttc ctcgacgact
     1921 cctatgcgtc ggttgcgatc atcgacagcg cgttcctcga cgtggtcgga cctcaggtgg
     1981 cggcacgacc ctgggttcgc ctggtcgtgg ccaatggcac cgggccggaa ggcaccgatt
     2041 cattcgacgc ctggatcgaa gagaccgggg aggagatcga tccggttcgc acccaccccg
     2101 acgacatggc cttttggctg tacagctcgg ggtcgacggg gagacccaaa ggtgtggttc
     2161 accgccatgc cgatgtggca gccacttgca cccactacgc cgaaccggtg ctcggcatcg
     2221 ggccgggcga cgtggtgttc tccagcacca agatgttcca cgcctacggg ctcggcaaca
     2281 atctcacgtt cccgatgtgg tcgggggcga cctccgtgta ctcgatcggg cgtcccacac
     2341 cagatcgcct cctggagcgg gtccgaacgc atcgaccgtc gttgtacttc tcggtgcccg
     2401 ccctgtacaa cgccatcctt gcccacgccg agttcgacga caccgattgg tcgtctgtcc
     2461 gtctcgggat ctcggcagcc gagccgcttc ctcctgagat ctggcgccgc ttccacgatc
     2521 ggactggtgt cgagatcctc gacggtgtgg ggtcgaccga gatgctgcac atctattgct
     2581 ccaatcgggc aggtgccgtc agaccaggaa cctcgggcac gccggtggac ggatatgaaa
     2641 tggaggtccg cgatccggag ggatccctct gcccggtcgg ggaggcgggc gagatgtggg
     2701 tccgtgggcc gagcgctctc accatgtact ggcaccagca ccagcggagc caggacaaga
     2761 tccgtggcgc ctggttcctc tcgggtgaca ggtttcggca ggacgaggat ggctacttct
     2821 ggtacgaggg ccgcgtcgac gacatgatga aggtcggcgg tctgtgggtg agcccgatcg
     2881 agatcgaaaa ccgcctcgtg gagcacccga cggtgcggga ggcggcggtg gtggcgatcg
     2941 acatcgacca cctgtcacgc atcaaagccg tggtgatact cgccgaggga gtgacgcctg
     3001 gggaagacct cgacgccgag ttgcaggcat ggtgcaagtc cgggctgcag cggtaccagt
     3061 acccccatgt catcgaatac gtcgatgatt tcccccgaac tgcgaccggg aagatccagc
     3121 gcttcagact g
//
LOCUS       DASTXO010000167         4753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1041697, whole genome shotgun sequence.
ACCESSION   DASTXO010000167 DASTXO010000000
VERSION     DASTXO010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041697"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1623
                     /locus_tag="VFU96_03580"
     CDS             <1..1623
                     /locus_tag="VFU96_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HEU5112371.1"
                     /translation="AVGIAQAVANRGDDAVVAVYGGEGSTSEGDWHEAMNWAGIQRLP
                     LIFVIENNHYAISVPQEEQVAGSLAARAESYGFPGVEVDGNRTLRVYKVMQEAVRRAR
                     AGEGPTLIEAETYRYYAHTSDDNDTLYRSREEVETWRKRDPVARVRQYLIENRLLTEA
                     DEERIDNEVIEALARAVETAEAGPNPDEATSRVYARVVEPGPPVTKPEEPVGGESVNL
                     ITAINMALHEVLEAHEDAVVFGEDVARRKGGVFKATQDLTDRFGPERCFNTPIAESSI
                     IGAAIGMAAAGFKVIPEIQFADYIHPAFDQLVSEAARISFRSDGEWNVPMVVRTPYGA
                     GIHGALYHSQSIEAFYAHVPGLKVVVPSTPADVKGLLFTAVEDPDPVMFLEPKKLYRL
                     AKGPYPEGEHRIPLARAAIRHQGEDLTIIAYGAMAHFAVEAASLLEELDISPEVIDLR
                     SLKPLDWPTIEASVKKTSRALIVYEDNEFAGYGAELAAQIADKTFEWLDAPVRRYALP
                     DVPIMPYAGPLENELYPTPEGIVQKAQELAKY"
     gene            1664..3427
                     /gene="argS"
                     /locus_tag="VFU96_03585"
     CDS             1664..3427
                     /gene="argS"
                     /locus_tag="VFU96_03585"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011644150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU5112372.1"
                     /translation="MSLLSQLSTVAGEAFIELGFSPELGETVPSQRPELAQFQVNGAM
                     AAGKSTNQPPRDIAQKVAERLARHPDIADVGVAGPGFINITLTDAAIARIASEDAADP
                     YLGFPVTDQPENVVVDYAGPNVAKAMHVGHLRATIIGDSLARLFSFAGHKVIRDPHFG
                     DWGFQMGLLIAAIGDEQPELPYFDPGAGTYPSESPVTLEDLQRIYPEASVRARSDDDF
                     ADDARRATVRLQQGDPGYLALWRHMKSVSEESQRQDFAALGVNFDLWFGESDVHDRLG
                     PLVTSLRESGVAEESKGAIVIRVGEDEDNRDIPPLILESSSGGFLYSTTDLATIQMRM
                     EDLRADLVLYVVDRRQADHFLQIFRAAIKSGIVPEGTRLEHIWFGTMNGPDGKPFKTR
                     EGGVVRLSDVIEMLTDAARRRLDEAHLAEDYPVEERSNIASMVGVAALKFGDLINNRT
                     SDYVLDLDRFSSFEGKTGPYLQYAAVRIKSILRRAETEGVEPGPMQAPSVDAERELML
                     ELLRLPEVVGRAIALRSPNHVAEFAFEVAGRFNRFYDACHILSEPDIARRGSWLSLAS
                     WALAALERLLDLLGIEVPDRM"
     gene            3430..4272
                     /locus_tag="VFU96_03590"
     CDS             3430..4272
                     /locus_tag="VFU96_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5112373.1"
                     /translation="MAFVIRPARAEDVASIRPWTQDTFSWGDYVPEMIGGWLDEPDSL
                     VIVCAHEDDIPVAVSRVQLLSPTEAWLSATRVHPDERRSGMGMAMNDHAVEWAKSRGA
                     LVARLVIEESNQAARGQVLRSGYREGGRWAHATAPESFGRRLPPDQRLRAAATVDADA
                     AWTFWSQSDLAQAARDLISVGWRWRKASRQDLETAVAAHTFYQSPGGWVIAEADAEGI
                     SARWPATTAPDAPLLFQGLRDLLRDRGGGRVEAMIPITVWSVEALQREGFEVHPAQIF
                     TKAL"
     gene            complement(4323..>4753)
                     /locus_tag="VFU96_03595"
     CDS             complement(4323..>4753)
                     /locus_tag="VFU96_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112374.1"
                     /translation="VNVVQDLSTGAMLASSLVLAVIGLFCLGVAAEGPLGRGRRLVGF
                     KLWEIGLGRIVAVFVVGLLFLLVHNLVDGAVEGLPSPVTTGVAGIRAVGVAGMTVMPL
                     LGVPIALAARWYPDGPDWLRSTEIPIMFVVWAVATMAIVS"
BASE COUNT          851 a         1558 c         1553 g          791 t
ORIGIN      
        1 gccgtgggca tcgcccaggc ggtggccaac cggggcgacg acgcggtcgt tgccgtgtat
       61 gggggtgagg gctcgacgtc cgagggggac tggcacgagg ccatgaactg ggccgggatc
      121 caacggttgc cactgatctt cgtgatcgag aacaaccact acgccatctc cgtcccccag
      181 gaggaacaag tcgccgggag cctcgccgcc cgggctgaga gctatgggtt cccgggtgtc
      241 gaggtcgacg gcaaccggac acttcgtgtg tacaaggtga tgcaggaggc ggtacggcgg
      301 gcgcgggcgg gcgagggacc gacgctgatc gaagcggaga cgtatcgcta ctacgcccac
      361 acctccgatg acaacgacac gctctaccgg agccgggagg aggtcgagac ctggcgcaag
      421 cgtgacccgg tggcgcgtgt ccgccagtac ctgatcgaga acaggctgct caccgaggca
      481 gacgaggagc gaatcgacaa cgaggtgatc gaggccttgg ctcgggcggt ggagaccgcc
      541 gaggccggcc ccaatccaga tgaagccact agccgcgtgt atgcccgggt cgtcgagccc
      601 ggccctccgg tcaccaagcc ggaggagcct gtcggggggg agtcagtgaa cctcatcacg
      661 gctatcaaca tggcgcttca tgaggtcttg gaggcacacg aggacgctgt ggtgttcggc
      721 gaggacgtgg cccgacgcaa gggtggggtc ttcaaggcga cgcaggacct caccgaccgc
      781 ttcgggcctg agcgctgctt caacaccccg atcgccgagt catcgatcat cggcgccgcg
      841 atcggcatgg ccgcggccgg attcaaggtc atccccgaga tccagttcgc cgactacatc
      901 cacccggcct tcgaccagct ggtctccgag gcggcgcgaa tctcgttccg ctccgacgga
      961 gaatggaacg tgcccatggt ggtgcgcact ccgtacggcg cggggatcca cggggccctc
     1021 tatcactcac aatcgatcga ggcgttctac gcccatgtgc cagggctgaa ggtggttgtg
     1081 ccttccaccc cggcggacgt caaagggctg ctcttcaccg cggtcgaaga ccccgatccg
     1141 gtcatgttcc tcgagccgaa gaagctctat cggctggcga aaggccccta tccggaaggc
     1201 gagcatcgga tcccactggc ccgggcggca atccgccacc agggtgagga cctgaccatc
     1261 atcgcctacg gggcgatggc ccatttcgcg gtggaggctg cgtccttgct cgaggagctc
     1321 gacataagcc ccgaggtgat cgatctgcgc agcctcaaac ccctcgactg gcccacgatc
     1381 gaggcgtcgg tgaagaagac ctcacgagct ctcatcgtct acgaagacaa cgagttcgcc
     1441 ggttacggcg ccgagcttgc ggcgcagatc gctgacaaga ccttcgaatg gctcgatgca
     1501 ccggtgcgtc gctacgccct tccagatgtg ccgatcatgc cctacgccgg accgctggag
     1561 aatgagctct accccacccc cgagggcatc gtccagaagg cccaggagct ggcgaagtac
     1621 tgacgttctt cgtttttcct tttaccttct acgttgtgcg tgaatgtcgc ttctgagtca
     1681 gctctcgact gttgccggtg aggccttcat cgagctcggt ttctcccccg agctgggtga
     1741 gacggtccct tcccagagac ccgagctggc tcagttccag gtaaacggcg ccatggcggc
     1801 aggaaagagc acgaaccagc cgccgcggga tatcgcccaa aaggtggccg agcgtcttgc
     1861 cagacaccct gacatcgcgg atgtcggagt ggccggcccg ggtttcatca acatcacact
     1921 gaccgacgcc gccatcgccc gcatcgcctc ggaggacgcc gcggacccct atctcggttt
     1981 tccggtgacc gaccagcccg aaaacgtcgt cgtcgactac gccgggccca acgttgccaa
     2041 ggcgatgcat gtgggccatc tccgggcaac gatcatcgga gactcgctcg ccagactctt
     2101 ttcgtttgcc gggcacaagg tcatacgcga ccctcatttc ggcgactggg gctttcagat
     2161 gggcctgctc atcgccgcca tcggcgacga gcagccggag ttgccttatt tcgaccccgg
     2221 ggccggcacc tatccatcgg agtcaccggt cactctcgaa gaccttcagc ggatctaccc
     2281 cgaggcctcc gtacgggccc gtagcgacga cgacttcgcc gacgacgccc gtcgagccac
     2341 cgtgcggctc caacagggtg atccgggata tctcgccctc tggcgacaca tgaaatcggt
     2401 gtccgaggaa tcccagcgcc aggacttcgc cgccctggga gtcaacttcg acttgtggtt
     2461 cggggaatcc gacgtgcacg acaggctcgg gccgctagtg acttcgttgc gcgagtccgg
     2521 ggtagccgag gagtcgaagg gtgccatcgt gatccgggtc ggggaggacg aggacaaccg
     2581 ggacattccg cccctgatcc tcgagtcttc aagtggcggg tttctctaca gcacgactga
     2641 cctggccacg attcagatga ggatggagga cctgcgcgcc gacctcgtcc tctacgtcgt
     2701 cgataggcgg caggcggatc atttcctcca gatcttccgg gcagcgatca agtccggaat
     2761 cgtccccgag ggcaccagat tggagcacat ctggtttggg accatgaatg ggccggacgg
     2821 caagccgttc aagacacgtg agggtggagt cgtccgcctc tccgacgtga tcgagatgct
     2881 caccgacgcc gcccggcggc ggctcgacga ggcgcatctc gccgaggact atcccgtcga
     2941 ggagagatcg aacatcgcct ccatggtcgg tgtagccgct ctcaagttcg gcgatctcat
     3001 caacaaccgc acgtcggatt atgtcctgga tctcgaccga ttcagctcct tcgaggggaa
     3061 gaccgggccc tatctccaat acgcggccgt gcggatcaag tccatcctgc gccgggcaga
     3121 aacggagggg gtggagccgg gcccgatgca ggccccgagc gtcgatgccg agcgcgagct
     3181 catgctcgag ctccttcgtc tccccgaagt ggtcggtcgt gccattgccc tgcggtcacc
     3241 gaatcatgtc gccgagttcg cattcgaggt ggccggtcgt ttcaaccgtt tctacgacgc
     3301 ctgccacatc ctctccgagc cagacatcgc acggcgcggt tcctggctct ccctggcctc
     3361 ctgggccctg gccgcgctcg agcgcctgct ggacctcctc ggcatcgaag tccccgatcg
     3421 gatgtagtca tggcgttcgt catccgcccc gcccgggccg aggacgtcgc gtcgatcagg
     3481 ccgtggactc aggacacctt cagttgggga gactatgtcc ccgagatgat cggcggctgg
     3541 ctggacgagc ccgattcgct ggtcatcgtc tgcgcccatg aggacgacat cccggttgcg
     3601 gtctcacggg tgcagctcct ctccccgacc gaggcctggc tgagcgccac ccgcgtccat
     3661 cccgacgagc ggcgatcggg tatggggatg gccatgaacg accacgcggt cgaatgggcc
     3721 aagtcgaggg gggctctcgt cgcccgactc gtcatcgagg aatcgaacca ggccgcccgc
     3781 ggccaggtgt tgcggtcggg ctaccgggag ggaggtcgct gggcccacgc cacagcgccg
     3841 gagtcgttcg gacggcgtct gccgccggac cagcgtttga gagcagctgc caccgtggat
     3901 gcggacgccg cctggacctt ctggtcacag agcgatctcg cccaagccgc ccgggacctc
     3961 atctctgtgg gctggcgctg gaggaaggcc tcccgccagg atctggaaac ggcggtcgcg
     4021 gcccatacct tctaccagag cccaggggga tgggtcatcg ccgaggccga tgctgaaggg
     4081 atctcggcac gctggccggc aacgacggcg ccggacgctc cgctgctctt tcaggggctg
     4141 cgagacctgt tgcgagatcg cggtggaggt cgggtcgagg cgatgatccc gatcactgtc
     4201 tggagcgtcg aggccttgca gcgagagggc ttcgaggtgc acccggccca gatcttcacg
     4261 aaggctctct gacctgcccc ggccctgcgg ggcaacctcc gcgactcccc tgggcggaag
     4321 gctcaggaca cgatggccat cgtggccacg gcccagacga cgaacatgat ggggatctcg
     4381 gtggagcgga gccagtcggg gccatcggga taccagcgtg cggcgagggc gatcgggacg
     4441 ccgaggagtg gcatgacggt catcccggcg acgccaactg cccgtatccc ggcaaccccg
     4501 gtggtcaccg gcgacggcag accctcgacc gcgccatcga ccaagttgtg gacgagcagg
     4561 aacaggaggc ccactacgaa gacggcgacg atccggccca agccgatctc ccagagcttg
     4621 aagccgacaa ggcgccggcc ccttcccaat gggccttcgg ccgctacacc caggcagaac
     4681 aggccgatca ccgcaaggac gagcgacgag gccagcatgg ccccggtgga aaggtcctgg
     4741 accacgttga cga
//
LOCUS       DASTXO010000168         4470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_857846, whole genome shotgun sequence.
ACCESSION   DASTXO010000168 DASTXO010000000
VERSION     DASTXO010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857846"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..590)
                     /locus_tag="VFU96_03600"
     CDS             complement(144..590)
                     /locus_tag="VFU96_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112375.1"
                     /translation="MSASLLAFVTLLVGVFLVIVALVVWQEARRRPSYEPLEYVIEDA
                     VKHVETGLESEGKDRLSRGDIRRILEWEVFYLQGLAQDDRKNPVETIAGGHEASIEYI
                     SEQIATKHGVSYSHDDVEDVLRLEADYLYRIGAVGERVDPDGGEDE"
     gene            complement(613..2409)
                     /locus_tag="VFU96_03605"
     CDS             complement(613..2409)
                     /locus_tag="VFU96_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011290657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEU5112376.1"
                     /translation="MASHYRSNLRDLEFNLFEVNRIQDYLGTGPWPDIDEASARDILV
                     EIERLAREDFAASYVDQDRIPLVLEDGEVHLPDSVKAGVRAYKEGGWDRFAVPADMGG
                     MPVPPSLAWATQEMQLAANTTVSWYSGGALFATVLFEEGNEQQKDLARLWLDKQWSGS
                     MILTEPDAGSDVGSASTRAVDMGDGTWHIEGVKRFITGGEHDASENIIHLVLARPVGA
                     VPGTKGLSLFVVPKFLINEDGSLGPRNGVVATNIEKKMGLKGSTTCEMTFGADDPAIG
                     YLVGDKHDGIRQMFQVIESARMMIGTKSMGTLSTGYLNALEYAKERRQGADLTLATDK
                     TAPRVPIIDHPDVRRMLMNLKAYSEGLRALVLYTAWVQDQARLHPDDDDWERRSDLLL
                     PLVKGYSSEKAYEQLADALQIFGGSGFTQDYPLEQYIRDTKIDTIYEGTTGIQSLDFF
                     FRKIARDQGGSIAALVAEITEFVKGGADGDPIATERERLGTMLDDLQGHLGSMVEHLM
                     ASLAGRSEEIYKVGLHTNALLASTAELVIAWQLLRHAEIAYDKIDADPFYRGKVASAR
                     WFLDMAAPEIAARRSAAETEDGALMSLPVEAF"
     gene            2556..3116
                     /locus_tag="VFU96_03610"
     CDS             2556..3116
                     /locus_tag="VFU96_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU5112377.1"
                     /translation="MRYKAGLETRERILEATRSLVAENGLDGTTIKAICAQAGVLPGS
                     FYNLFASKEQAIVTVVREAIDAVDPDPAHQGTDSLDDLVAAYVRFLEESPALSRVYIR
                     IGVSGGGSNPELSGRMIRHHQARVARFAAAMKRQSPELSETLAERRAETLVLALNGIA
                     LHRIVDPDFDVSWHARALLEDAAAVS"
     gene            3146..4060
                     /locus_tag="VFU96_03615"
     CDS             3146..4060
                     /locus_tag="VFU96_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5112378.1"
                     /translation="MDLAVQTRGDWELVLTAARWAEDRGLVAFALPDHYLERGDETDC
                     PAYDHLVHLAALARETSKIELVSLLSPVTFRHPAVYYKMGVTLDEVANGRFTMGIGTG
                     WLDEEFTLFGLPYPDRSTRFEMLDECLAYLTAAISPGGEGFDGKYYRLSRFESQPHPQ
                     NLKLLVGGGGRRTTPTLAGRYADEFNIYACRPEEYVARRELAEKSAAEKGRDPGSILF
                     STASPAVAARKESDYRRLLALLADRTTSTPARIEQVYEERGYPHGSGSKPAEMLKALE
                     EAGCQRFYPQMFLRNPGDFDIILDAYRP"
     gene            4063..>4470
                     /locus_tag="VFU96_03620"
     CDS             4063..>4470
                     /locus_tag="VFU96_03620"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEU5112379.1"
                     /translation="MPSVAILGTGLIGASVGLALREQGWGSVGWDPSPVALELSSERH
                     ATDSAGSVADAVDGADLIVLAGPLHANLETLSHLETGALVTDVTSVKLPLVGAGRNGV
                     RFVGGHPMAGREHAGPEAASAALFRGAPWVVCDD"
BASE COUNT          775 a         1468 c         1394 g          833 t
ORIGIN      
        1 gggggcggtc gggtacccgc ccatcgtcga gggctcggcg ccccccggct cacgcaccac
       61 gttgaccgac ccgcagaccg ggcacgcaaa acgccccggc cccgggacct cgaactgcgt
      121 ccggcaggca ccgcaacgca ccatcattcg tcctcacctc catcggggtc gaccctctcg
      181 cctacggcgc cgatgcgata caggtagtcg gcttcgaggc ggaggacatc ttcaacgtcg
      241 tcgtgagagt aggacacgcc gtgcttggtg gcgatctgct cggagatgta ctcgatcgaa
      301 gcctcgtggc cacctgcgat cgtctccacc gggttctttc gatcgtcctg agccaggccc
      361 tggagataga acacctccca ttccaggatt ctccggatat ccccccggct cagccggtct
      421 ttgccctcgg attcgaggcc ggtctcgaca tgcttcacgg catcctcgat gacgtactcc
      481 aggggctcat agctcggtcg ccgtctcgcc tcctgccaca cgacaagggc gacgatgacg
      541 agaaacacgc ccacgagcag tgtgacgaag gccaacagcg atgccgacac catgcagaag
      601 gtacccggcg ggctagaacg cctccaccgg caggctcatc aatgctccgt cctcggtctc
      661 ggccgcggat cggcgtgctg cgatctctgg tgcagccata tcgaggaacc atcgggccga
      721 tgcgactttg ccccgataga acggatcggc gtcgatcttg tcgtacgcga tctcggcgtg
      781 gcgcagcaac tgccaggcga tgaccagctc ggccgtggag gcgagcagtg cgttggtgtg
      841 gagacccacc ttgtagatct cctctgaccg gccggccagc gacgccatga gatgctccac
      901 catcgacccg aggtgaccct ggaggtcgtc gagcatcgtt ccgagacgct cccgctcggt
      961 cgcaatcggg tcgccgtcgg ccccgccctt cacgaattcg gtgatttcgg cgacgagagc
     1021 agcgatcgac ccaccctgat cccgtgcgat cttgcggaag aagaaatcga gcgactgaat
     1081 gccggtcgtg ccctcgtaga tggtgtcgat cttggtatcc cggatgtatt gctccagcgg
     1141 gtaatcctgg gtgaacccgg agccaccgaa tatctggagg gcgtcggcca gctgctcgta
     1201 cgccttttcc gaggagtagc ccttgaccag cggaagcaat agatccgacc ggcgctccca
     1261 gtcatcgtca tccgggtgga gccgggcctg atcctggacc cacgccgtgt acaagacgag
     1321 ggctcgcagc ccctcggagt aggccttcag attcatcagc attcgacgga cgtcggggtg
     1381 atcgatgatg gggacccgtg gcgccgtctt gtcggtggcg agcgtcaggt ccgcgccctg
     1441 acgacgctcc ttggcgtact ccaacgcatt gagatacccg gtgctcaggg tgcccatcga
     1501 tttcgtgccg atcatcatgc gagcactctc gatcacctgg aacatctgcc ggatgccgtc
     1561 atgcttgtcg ccgacgaggt agccgatcgc cggatcgtcg gccccgaatg tcatctcaca
     1621 ggtcgtcgag cccttcagcc ccatcttttt ctcgatgttg gtggcgacca ccccgttgcg
     1681 ggggccaagc gatccgtcct cattgatcag gaacttcggc accacgaaga gagacagccc
     1741 ttttgtcccg ggaacagctc ccacggggcg ggcaagcacg aggtggatga tgttctctga
     1801 ggcatcgtgt tccccgccgg tgatgaatct cttcactcct tcgatgtgcc acgtgccgtc
     1861 gcccatgtcg acggccctgg tcgacgccga ccccacgtcg gacccggcgt ccggctcggt
     1921 caggatcatc gagcccgacc attgcttgtc gagccagagc ctggcgagat ccttctgttg
     1981 ctcgttcccc tcctcgaaga gcacggtggc gaagagggcg ccgccggaat accacgacac
     2041 agttgtgttg gccgccagct gcatctcttg ggtcgcccag gcgaggctgg gcgggacagg
     2101 catgcccccc atgtcggccg gaacggcgaa tcggtcccag cccccctcct tgtaggcgcg
     2161 cactccggct ttgaccgagt cgggaagatg cacctcgccg tcttcgagca caagcgggat
     2221 ccgatcctga tcgacgtagg acgcagcgaa atcctcccgg gccagccgct cgatctcgac
     2281 gaggatgtca cgcgcggatg cctcgtcgat gtcgggccac ggcccggtgc ccagatagtc
     2341 ctggatccgg ttgacctcga agaggttgaa ctcgagatcc cgcagattcg acctgtagtg
     2401 gctcgccatt tccccttcct gatctttgag cgttgactct aatttgaatc aacgctcaaa
     2461 taaagggtca tgttccggga cccccaaccc actcggtcac ggttacgttg cggccaagca
     2521 gcgggaatgg cagccgcacg gtctaccgtt tccccatgcg gtacaaggca gggttggaga
     2581 cgcgggagag gatcctcgaa gccacccgat cactcgttgc ggagaacggg ctggatggga
     2641 ccacgatcaa ggccatctgt gcgcaggccg gagtcctacc aggctccttc tacaaccttt
     2701 tcgcctcgaa ggaacaggcc atcgtgaccg tggttcgcga ggcgatcgac gccgtcgacc
     2761 ctgatccggc acatcagggc accgactctc tcgacgattt ggtcgcggca tacgtgcgtt
     2821 ttctcgagga atccccggct ctgtcccggg tctacatacg catcggggtg tccgggggcg
     2881 ggtccaaccc ggagctcagt ggccggatga ttcgtcacca tcaggctcgg gtggctcgct
     2941 ttgccgccgc gatgaagcgc caaagccccg agctctcgga gaccctcgcc gaaagacgcg
     3001 cggagacgct ggtgctggcc ctgaacggca tcgccctcca tcgcatcgtc gaccccgact
     3061 tcgacgtctc gtggcatgcc agggcccttc tcgaggacgc ggcggcagtt tcctgacctc
     3121 cgagtggcgt cgataggctc agaacatgga tctcgccgta cagactcgtg gcgactggga
     3181 gttggtcctg actgccgccc gctgggcgga ggatcgtggg ttggttgcct tcgcgctccc
     3241 tgatcactac ctggagcgag gagatgagac cgactgtccc gcctacgacc atctggtgca
     3301 cctcgccgcc ctcgcccggg agacgagcaa aatcgaactg gtctcactcc tctccccggt
     3361 cacctttcgc catccggcgg tgtactacaa gatgggtgtg accctcgatg aggtcgccaa
     3421 cggccggttc acgatgggaa tcgggacggg gtggctggac gaggagttca cgttgttcgg
     3481 tctcccctac ccggaccgat ccacccgatt cgagatgctg gacgagtgct tggcctatct
     3541 caccgcggcg atatcgcccg gcggcgaagg gttcgacggc aagtactacc ggctatcacg
     3601 tttcgagtcc cagcctcatc cccaaaacct gaaactcctg gttggcgggg gtgggaggcg
     3661 tacgacgccg acactggccg gtcggtacgc cgacgagttc aatatctacg cctgcaggcc
     3721 cgaggaatac gtggcgagac gagagcttgc cgagaagagc gcggccgaaa aagggcgcga
     3781 tcccggctcg atccttttct cgacagccag tcccgcggtg gcagctcgca aggagtccga
     3841 ctatcgccga ttgctggcgt tgctcgctga tcggaccacg tcgacaccgg cgcgcatcga
     3901 gcaggtctac gaggagcggg ggtatcccca cggctcgggc tcgaagccgg cagagatgtt
     3961 gaaagcgttg gaagaggccg gctgccagcg cttctacccg cagatgttcc tccgcaatcc
     4021 cggggatttc gacatcatcc tcgacgccta ccggccctga ccatgcccag cgtcgccatc
     4081 ctcgggaccg ggctcatcgg tgcatcggtt gggctcgccc tcagagagca gggatgggga
     4141 tcggtgggtt gggacccctc gccagtggcc ctggaactgt cttccgagcg gcacgccacg
     4201 gacagcgcag gctccgtggc ggatgccgtg gacggggcag acctgatcgt cttggcaggc
     4261 cctctgcatg ccaatctcga gaccctctcc cacctcgaaa ccggggcctt ggtcaccgat
     4321 gtgaccagtg tcaagttgcc cttggtcggc gccggccgca acggggtccg ttttgtgggc
     4381 ggtcatccca tggcgggacg cgagcacgcc gggcccgagg cggcgtccgc ggcgctgttc
     4441 cgtggcgctc catgggtggt ctgtgacgat
//
LOCUS       DASTXO010000169         3138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1705249, whole genome shotgun sequence.
ACCESSION   DASTXO010000169 DASTXO010000000
VERSION     DASTXO010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1705249"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="VFU96_03625"
     CDS             <1..815
                     /locus_tag="VFU96_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU5112380.1"
                     /translation="NTVPWFEEPGFDPSTIDSLDGVLEALRAAQAARPDGIPLTVQAL
                     PTTGVTLFKTFLSAAGAPFEGAVPDLQSPGGIYTLEWFLTAAAEGLIPPDAISWDEDQ
                     ARGVFLAQDAAMILDGFTVAGDLGEEPGFDYETVWSVIPVPASQTGAQKDGNQISNAR
                     TWFLTSGTEHPYEAGLAMRYVNETLVDAAANGSVPMRNSRHLADPRLDEIWPFFTDPL
                     REAYAGSDSVPAGLNGGDVEAVLEQMFGEIVVGTDKTAQELADEYQPQLDEL"
     gene            894..1835
                     /locus_tag="VFU96_03630"
     CDS             894..1835
                     /locus_tag="VFU96_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU5112381.1"
                     /translation="MTAIAERARRQREPRPSDPNNSVMLGQGRSTPYLFSLPTIIVVA
                     AVLAFPVLYGVWESLFRPEILGAPEEWVGLENYADMFQDEMFWSSLVRTFIFVGGCLI
                     VGTTLGLLFAFALFQVIGRARFLRSISLAPYLISNVAAALMFRILFNSQFGLVNGLLG
                     QIGIDGPSWLSQPVTAMLVVIFAQVWTDLPLTILLLLGGLMTIDKSYLDAALVDGATG
                     WTRARYVTMPLLTPQIVISTVWLSYSTLTGLGVVLALTGGGPLNATTTLAMDMYLTAF
                     RSLEFNRALAIATFILVLNALLTLSYVRVSRRYEVAL"
     gene            1835..2731
                     /locus_tag="VFU96_03635"
     CDS             1835..2731
                     /locus_tag="VFU96_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU5112382.1"
                     /translation="MVGTKTREEAGPTVRGSGTPGLSRRGRWVRRLVLSLPLIALVLW
                     TVLPFLVTISVSFKTKGETFANYNLIPENPTIQAYQQVLADPNFRSAFWNSVIVGFGT
                     ALLTILLAVPAAYAFARFRFRGRHLLLLLVLLPRLVPSLGTMVPLYRLAAATGQLDKL
                     TTLIFVYTGTLLPLAVWLLVGFIQQIPRDIEEAASVDGATLWQRLRGVVLPLAAPATI
                     TIAALAFREAWNEFTLALVLTSTPGRRTLPFELFMLQTGQGIANFPAEAAFALLTVLP
                     FLIMYNRIERYMVAGLVSGSAK"
     gene            2743..>3138
                     /locus_tag="VFU96_03640"
     CDS             2743..>3138
                     /locus_tag="VFU96_03640"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5112383.1"
                     /translation="MSSHRSIGLGVVGSGRMGTHRARLAAAHPGVDYLRVMDISAEAA
                     ERLAATTEADAFGDDLAALLDDPRVDAVIVSLPEDTHVDAVVAACASGRPVMVEKPLA
                     LEIAGGSKMVAEAAAAGVDLRVAYSARFLQ"
BASE COUNT          477 a         1098 c          965 g          598 t
ORIGIN      
        1 acaacaccgt cccctggttc gaggagcccg ggttcgaccc gtcgacaatc gactctctcg
       61 atggcgtcct cgaagcattg cgggcggccc aggcagcccg accggatggg atcccactca
      121 ccgtgcaggc cttgccgacc accggtgtca ccttgttcaa gaccttcctc agcgccgcgg
      181 gcgctccgtt cgagggtgcc gtccccgact tgcaatcgcc gggtggcatc tacaccctgg
      241 aatggttcct gaccgcggcc gccgagggtc tgataccccc ggacgccatc agctgggatg
      301 aggaccaggc ccgcggggtc ttcctggccc aggacgcggc gatgatcctc gacggattca
      361 cagtcgccgg cgacctcggt gaggagcccg ggttcgatta cgagaccgtt tggagcgtca
      421 ttcccgtccc ggcttcacag accggcgccc agaaggacgg gaaccagatc tccaatgccc
      481 ggacctggtt cctcaccagc ggcaccgagc atccatacga ggctggcctc gccatgaggt
      541 acgtcaacga gaccctggtg gacgccgcgg ccaatggttc ggtgccgatg cggaactcga
      601 ggcacctcgc cgatccccgg ctggatgaga tctggccgtt cttcaccgat ccgctccgtg
      661 aggcctacgc cggaagcgac tcggtcccgg cggggctcaa cggtggcgat gtcgaagccg
      721 tgctggagca gatgttcggt gagatcgttg ttggcacgga caagaccgct caagagctgg
      781 cagacgaata ccagccacag ctcgacgagc tgtaagcccg atgacctgcg tgcccgtccc
      841 ggtgagccgg ggcgggcgcg tcggccgccg gccagaggac tgagagagga caaatgacgg
      901 ccatcgccga gcgggcccgc cgacaacggg agccacgtcc ttccgacccc aacaacagcg
      961 tcatgctcgg ccagggccgg tcgactccgt acctgttctc gttgccgacg atcatcgtcg
     1021 tggcggcggt gctcgccttc ccggtcctgt acggagtctg ggagagcctg ttccgacccg
     1081 agatcctcgg cgctcccgag gagtgggtcg gcctggagaa ctacgccgac atgttccagg
     1141 acgagatgtt ctggagctcc ctggtgcgga ccttcatctt cgtcggtggc tgtctgatcg
     1201 tcggaaccac tctcgggctg ctcttcgcct tcgccctgtt ccaggtgatc gggcgggcgc
     1261 gattcctgag atcgatctcg ctcgccccgt acctgatctc caacgtggcg gctgccttga
     1321 tgttccgcat cctgttcaac tcccaattcg gcctggtgaa cggattgctc ggccagatcg
     1381 gtatcgacgg gccgtcgtgg ctgtcccagc cagtgacggc gatgctggtg gtcatcttcg
     1441 cccaggtatg gaccgatctc cccctgacga tcctcctcct cctcggcggg ttgatgacga
     1501 tcgacaagtc gtacctcgat gcggcgctcg tcgacggcgc cactggctgg acgcgggcga
     1561 gatacgtcac catgccgctg ctcacccccc agatcgtgat cagcaccgtc tggctgagct
     1621 actcgaccct caccggcttg ggcgtggtcc tggcgttgac cggaggtggg ccgctcaatg
     1681 ccaccacgac actcgccatg gacatgtacc tgaccgcgtt cagatccctc gagttcaacc
     1741 gggccctggc catcgccacc ttcatcctgg tgctcaacgc cctgctcacc ctttcctacg
     1801 ttcgggtctc ccgtcgatac gaggtggcgc tctgatggtg gggacgaaga ctcgcgagga
     1861 ggctgggccc accgtcaggg gctcgggcac ccccggcctg tcgcgtcggg gccggtgggt
     1921 acgccggttg gtgctctctc tccccttgat tgcgttggtg ttgtggacgg tcctcccgtt
     1981 cctcgtcacc atctcggtct ccttcaagac caaaggggag acgttcgcca actacaacct
     2041 gatcccggag aacccgacga tccaggccta tcagcaggtc ctggccgatc ccaacttccg
     2101 atccgccttc tggaacagcg tcatcgtcgg gttcggcacc gccctgctca ccatcctcct
     2161 cgccgtcccg gcggcgtacg ccttcgcccg cttccggttc cggggtcggc acctcctcct
     2221 gctcctggtg cttctccccc ggctcgtccc cagcctgggc acgatggtcc ccctctaccg
     2281 tctggccgcg gcaaccggac agctggacaa actcaccacc ctgatcttcg tgtacaccgg
     2341 gacgctcctc ccactggcgg tatggctcct ggtcggtttc atccagcaga tcccgcgcga
     2401 catcgaggag gcagccagtg tcgacggggc cacgctctgg cagcgtctcc gcggcgtggt
     2461 ccttcccctg gccgccccgg ccaccattac catcgccgcc ctcgccttcc gggaggcttg
     2521 gaacgagttc actctcgccc tggtcctgac ctctacccca ggtcgtcgga cccttccctt
     2581 cgagttgttc atgctccaaa cggggcaggg catcgccaac ttccccgcgg aggccgcgtt
     2641 cgccctcctc accgtgctgc ccttcctgat catgtacaac cggatcgagc ggtacatggt
     2701 ggccggtctc gtctccgggt ctgcaaaata ggagagtcaa tgatgagcag tcatcgcagc
     2761 atcggtctcg gtgtggtcgg ttcggggcgg atgggcaccc accgcgcccg gttggccgca
     2821 gcccatcccg gtgtggacta cctccgggtg atggacatct cggccgaagc tgcggagcga
     2881 ctggcagcca ccacagaagc ggatgcgttt ggcgacgatc tcgccgccct gctcgatgat
     2941 ccccgtgtcg acgcggtgat cgtgtcgctt cccgaagaca cccacgtcga tgccgtggtc
     3001 gcagcctgcg cctcggggag gccggtcatg gtggagaagc cgttggcact cgagatcgcg
     3061 ggtggatcga agatggtggc ggaagccgcc gcagccggcg tcgacctccg tgtcgcctat
     3121 tccgcccgct tcctgcag
//
LOCUS       DASTXO010000170         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1668111, whole genome shotgun sequence.
ACCESSION   DASTXO010000170 DASTXO010000000
VERSION     DASTXO010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1668111"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VFU96_03645"
     CDS             complement(<1..855)
                     /locus_tag="VFU96_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014697817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU5112384.1"
                     /translation="MTSEPQNAPASALDKESVEAPETTEPETQISVDVDEPLEALEEV
                     EVSGREALVAPDLSDLPDDIADDFERAIEATVLEFKEGDIVEGTVVSVDADGAMIDIG
                     YKSEGLIPTRELSIRNNVDPNDAVKVGERVEAVVLNKEDDEGRLILSKKRAMYERAWG
                     RIQEVAKEGGTVDGTVIEVVKGGLIVDIGLRGFLPASLVDMRRVRDLDPFIGESITAK
                     VIELDRARNNVVLSRRAYLEEAQAEERNAFLDGLGEGEVRDGVVSSVVNFGAFVDLGG
                     MDGLVHVSE"
     gene            complement(967..>2302)
                     /locus_tag="VFU96_03650"
     CDS             complement(967..>2302)
                     /locus_tag="VFU96_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEU5112385.1"
                     /translation="AALGTLPFDSGPDVETEGRRIEGVRRLVEVMRAELSDREEGELF
                     EEFELPLIPVLARMEHAGIAVDRAYLEDMGADLREKLTTLDARIQELAGEQFNVNSTD
                     QLRYVLFEKLALPVIKKTSTGKPSTDASVLKKLDHPVVEALLEYREYEKLRGTYVDGY
                     LPLVDGDGRIRTRFNQMAATTGRLSSDRPNLQNIPIRSESGRTIRRAFIAGEGAEFLV
                     ADYSQIELRVLAHMSGDPFLVEAFRSGSDIHTATAARVWGLSESEVSANQRRTAKMIN
                     FGLLYGMEAFGLSDRLGISREEAQQHMDAYFSQFVQVREFMSAVVTQARNQGYTTTLF
                     GRRRYLPELKSDNFRIRQMGERMALNAPVQGTAADIIKKAMIELDASLRSEGLASTLI
                     LQIHDELILESPLEELEVAEKLVVGTMEGVVSLDVPLEVDVATGPDLASVKG"
BASE COUNT          402 a          743 c          700 g          457 t
ORIGIN      
        1 ctcggacacg tggacgaggc cgtccatccc cccgaggtcc acgaacgccc cgaaattgac
       61 gactgatgag accacaccgt cccgtacctc accctcgccg aggccgtcga ggaacgcgtt
      121 gcgctcttcg gcctgggcct cttccagata tgcccggcgg gagaggacga cattgttcct
      181 ggcacggtcg agctcgatca ccttggcagt gatgctctcg ccgatgaagg ggtcgagatc
      241 acgcacacgc cgcatgtcga ccagtgaggc cgggaggaac ccgcggaggc cgatgtcgac
      301 gatgagacca cccttgacga cctcgatgac agtcccgtcc acagtgccgc cttccttggc
      361 gacttcctgg atccttcccc aggcccgctc gtacatggct cgcttcttcg agaggatgag
      421 ccggccctcg tcgtcctctt tgttgaggac cacggcttcg actcgctcgc ccaccttgac
      481 cgcatcgttg gggtcgacgt tgttccgtat ggagagctcg cgtgtcggga tgagcccttc
      541 ggatttgtaa ccgatgtcga tcatcgcccc gtctgcgtcc acgctgacga cggttccctc
      601 gacgatgtca ccctccttga actcgagaac ggttgcctcg atggcgcgct cgaaatcatc
      661 ggcgatgtca tcgggaaggt cgctcaggtc cggggcgacg agggcctcac ggcccgatac
      721 ttcgacttct tcgagagcct ctaggggctc atccacatcg acggagatct gcgtctccgg
      781 ctccgtggtc tcgggcgcct ccacgctctc cttgtcgaga gcgctcgcgg gcgcgttttg
      841 tggctcgctg gtcatgtgtg agtggttacc tccgaaggga actctgtaaa cgctgggtcc
      901 acaggcccga gtgagtgtac gcaggtttgt ccacaaccgc caatggattg gctcctggcg
      961 gggccgtcag cccttcacgc tggccaagtc tgggccggta gccacatcga cctcgagtgg
     1021 gacgtcgagg gagacgactc cctccatggt cccgacaacg agcttctcgg caacctcgag
     1081 ctcctcgaga gggctctcca ggatcagctc atcgtgtatc tggaggatca gcgtggaagc
     1141 cagaccctcc gatctcaggc tggcgtcgag ctcgatcatc gccttcttga ttatgtcggc
     1201 ggcggtcccc tgtacggggg cgttcagggc catccgctca cccatctgac ggatgcgaaa
     1261 attgtcggac ttcagctcgg gcagatagcg ccgtcgtccg aagagggtcg tggtgtaacc
     1321 ctggttccgc gcctgagtga cgacggccga catgaactcc ctgacctgga cgaactgcga
     1381 gaagtaggcg tccatgtgct gctgggcctc ttcgcgtgag atgccgagac ggtcggacag
     1441 gccgaacgcc tccatgccgt aaagcagtcc gaagttgatc atcttggcgg tgcgacgctg
     1501 atttgcggac acctcagact cggacaagcc ccaaacgcgg gccgcggttg cggtgtggat
     1561 gtcagagccg gaacggaacg cctcgacgag gaacgggtcc ccactcatat gagccagcac
     1621 tcgaagctcg atctgtgagt aatcggcgac gaggaactcg gccccctccc cggcgatgaa
     1681 tgcgcggcgg atggtgcggc ccgattctga ccgtatcggg atgttctgca ggttggggcg
     1741 atccgaagac aacctccccg tcgtcgccgc catctgattg aaccgcgtcc tgatccggcc
     1801 atcgccatcg accaaaggga ggtacccgtc gacgtacgtc ccccgaagct tctcgtactc
     1861 ccggtattcg agcaaggcct cgacgaccgg gtggtccagc ttcttcaaca ccgaggcgtc
     1921 ggtggacggc ttgcctgttg atgtcttctt gatcaccggc agtgccaact tctcgaacag
     1981 gacgtagcgg agctggtcgg tggaattgac attgaactgc tccccggcca gttcctggat
     2041 gcgggcatcc aaggtggtga gcttctctct caggtcggca cccatgtcct cgagataggc
     2101 acgatcgacc gctatgccgg cgtgctccat tcgggcgagg acggggatca aggggagctc
     2161 gaactcttcg aagagctccc cttcctcacg atccgatagc tcggctcgca tgacctcgac
     2221 cagtcgccgc accccttcga tccgacggcc ctccgtttcg acgtcgggcc cgctgtcgaa
     2281 cggcagcgtc cccaatgctg ca
//
LOCUS       DASTXO010000171         3472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_526488, whole genome shotgun sequence.
ACCESSION   DASTXO010000171 DASTXO010000000
VERSION     DASTXO010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526488"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /gene="metK"
                     /locus_tag="VFU96_03655"
     CDS             complement(<1..904)
                     /gene="metK"
                     /locus_tag="VFU96_03655"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU5112386.1"
                     /translation="MRSWYFTSESVTEGHPDKVADAISDSVLDAALANDKMARVACEV
                     FVTTRLAVVGGEISKGAEPDVAAIVDDTVRRIGYDGFDPGFAAGQLEIQDRVQQQSPD
                     ISDGVDRELGAGDQGLMFGFAVDETTELMPLPIQLAHRMAERLTEVRKTGKLEYLRPD
                     GKTQVMVRYEGDRPVAVEKVLISAHHARDKDQGEMRQEVFDAVVAAVIPEDMLDPHLG
                     FIFNPSGRFEVGGPMADTGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYGAR
                     WAAKNIVASGIARKVEIQLSYAIGQ"
     gene            complement(917..2095)
                     /gene="coaBC"
                     /locus_tag="VFU96_03660"
     CDS             complement(917..2095)
                     /gene="coaBC"
                     /locus_tag="VFU96_03660"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEU5112387.1"
                     /translation="MLQGRRVLLCVTGGVAAYKSAYLARRLIEAGATVRVQLTESALE
                     FIGPQTFAAITGEHPFTSLFAADLVSPHTELARWADLVIVAPATGATLGRVANGISED
                     LVSATIIATTAPVLLAPAMHTEMWEHPATGRNLETLRAFGYHVIGPEVGPLAGGDIGT
                     GRVAEPEEIVAAAGDILGGEGNGRKILVTAGGTREPVDPVRYLGNRSTGKMGHAIADA
                     AVRRGYDVTLVTTADLPTHPAVKLIRVETAEEMLTAVAGVEPDIAIMAAAVADFRPRS
                     ASKSKIPRADGLPSIELAETPDILASVMRRDPRPFTVGFAAETGGLERAVEKARKKDV
                     DILVYNDVTEPGSGFGTDTNRVVLIDRSGKTEDLPLLAKTEVAERLLDRVASDLGEQG
                     "
     gene            complement(2095..2316)
                     /gene="rpoZ"
                     /locus_tag="VFU96_03665"
     CDS             complement(2095..2316)
                     /gene="rpoZ"
                     /locus_tag="VFU96_03665"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEU5112388.1"
                     /translation="MKIEQVIDQIGNRFATVVVSARRARQINGYFHQLGVGYGEFVPP
                     QVHSLSRKPLTIAMEEIAEGKVSVDTVEE"
     gene            complement(2359..2913)
                     /gene="gmk"
                     /locus_tag="VFU96_03670"
     CDS             complement(2359..2913)
                     /gene="gmk"
                     /locus_tag="VFU96_03670"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015180146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU5112389.1"
                     /translation="MVAARLLVVSGPSGSGKSSIVRELIERLGIEFSVSATTRLPRPG
                     ERHGVHYNFISRRDFERMIENDELLEWAVYNNRYYGTPAALVEETLADQRDILLEIEI
                     QGARQVRERKPEAIMYFIAPPSLDELERRLRRRGDTSDEDIEDRLEIAQSEMAEAPEL
                     FDHIIVNDNLEQAISELEGLITAR"
     gene            complement(2915..3229)
                     /gene="mihF"
                     /locus_tag="VFU96_03675"
     CDS             complement(2915..3229)
                     /gene="mihF"
                     /locus_tag="VFU96_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HEU5112390.1"
                     /translation="MAQPPQLTDEQRTAALAKAAEARRVRAEAKELLKTGSLRLSELI
                     ERADSDDILAGLKVERVIASMPGTGKIKAKRLMESLGIAENRRIRGLGDKQKEALLAE
                     FS"
     gene            complement(3259..>3472)
                     /locus_tag="VFU96_03680"
     CDS             complement(3259..>3472)
                     /locus_tag="VFU96_03680"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase / HUMPS
                     family protein"
                     /protein_id="HEU5112391.1"
                     /translation="IVATPGIRPAGSEPADQRRVATPGEAIRAGADLLVVGRPITAAT
                     DPIEAAEAVSKEITAAVSAAAAGNAG"
BASE COUNT          560 a         1134 c         1115 g          663 t
ORIGIN      
        1 cctgcccgat ggcgtaggag agctggatct cgacctttcg cgcgatgccg gaagcgacga
       61 tgttcttggc agcccaccgg gcgccgtaag cggccgagcg gtcgactttg gttgcatctt
      121 tgccggaaaa ggcgccgcca ccatggcggg cgtacccgcc ataggtatcg acgatgatct
      181 tgcggccggt gaggccggtg tctgccatcg ggcccccgac ctcgaatcgc ccagacgggt
      241 tgaagatgaa cccgagatgg gggtccagca tgtcttcggg tatcacagcc gccaccacgg
      301 cgtcgaatac ctcctggcgc atctctccct ggtccttgtc ccgggcgtgg tgggcggaga
      361 tgaggacctt ttccaccgca accgggcggt caccctcata ccggaccatc acctgtgtct
      421 tgccatcggg gcgtagatac tcgagcttgc cggtcttgcg cacctcggtc agacgctcgg
      481 ccatgcggtg ggcgagttgt atgggcaggg gcatcaactc ggtcgtctca tcgacggcga
      541 agccaaacat gaggccctgg tcaccggctc cgagctcgcg atcgacccca tcggagatgt
      601 cggggctctg ctgttggacc cggtcctgga tctcgagttg accggcggca aaacccgggt
      661 cgaatccgtc gtagccgatg cgtcgaacgg tgtcgtcgac gatggcggcg acatcgggct
      721 cggctccctt gctgatctca cctccgacga ctgcgagacg ggtggtgacg aagacctcgc
      781 atgcaactcg ggccatcttg tcgttggcca gcgctgcgtc gagcaccgaa tccgagatcg
      841 catcggcaac tttgtcggga tggccttcgg tgaccgactc cgaagtgaaa taccagcttc
      901 tcatgcggca gcgaggttaa ccctgttctc cgaggtcgga ggccacgcga tcgaggagtc
      961 gttccgccac ttcggtcttg gcgagcagcg ggagatcctc ggttttgccg gaacggtcga
     1021 tcaggaccac ccggttggtg tcagtcccga atcccgaccc gggttccgtc acgtcgttgt
     1081 agaccaggat gtcgacgtcc ttcttccgcg ctttctcgac ggcccgctcc aggccacctg
     1141 tctctgcggc gaacccgacg gtgaagggcc tcgggtcgcg tcgcatcacc gaggcgagga
     1201 tgtccggggt ctcagcgagc tcgatcgaag gaagtccgtc tgctcggggg atcttggatt
     1261 tcgaggccga tcttggccga aagtcggcga ccgccgccgc catgatggcg atatcgggct
     1321 cgacgccggc gacggccgtg agcatctcct cggccgtctc cacccggatc agcttgaccg
     1381 ccgggtgggt cggaagatct gccgtcgtca ccaatgtcac gtcataccca cgtcgcaccg
     1441 ccgcatctgc gatggcgtgg cccatcttcc cggtcgagcg gttccccagg tagcgcaccg
     1501 ggtcgaccgg ctcgcgggtg cccccggccg tcaccaggat ctttcgcccg ttcccctccc
     1561 ctccgaggat gtccccggca gcggccacga tctcctcagg ctcggcgacc cggccggtcc
     1621 cgatgtctcc accggccaag ggcccgactt cggggccgat cacgtgatac ccaaaggcac
     1681 gcagggtctc gaggttgcgc ccggtggcgg gatgctccca catctcggtg tgcatcgccg
     1741 gtgccagcag gactggggca gtggtggcga tgatggtggc gctcaccagg tcttccgaga
     1801 tgccattcgc gacccggccg agtgtggcac cggtggccgg ggcgactatc accagatcgg
     1861 cccaacgggc caactcggtg tggggtgaga ccaaatctgc tgcgaacagc gaggtgaaag
     1921 ggtgttctcc ggtgatcgcg gcgaatgtct gcgggccgat gaactcgagt gcagactccg
     1981 tcagctgcac ccgcaccgtg gctccggcct cgatgagacg ccgggccagg taagcggatt
     2041 tgtaggcggc gacgccgccg gtgacacaga gaaggacgcg gcggccctgc agcatcactc
     2101 ttctacggtg tcgaccgaga ccttgccttc cgcgatctcc tccatggcga tcgtcaacgg
     2161 cttgcgggac aacgagtgga cttgaggcgg gacgaactca ccatatccga ctccgagctg
     2221 gtggaaatag ccgttgattt ggcgggccct gcgggccgag acgacgaccg tcgcgaagcg
     2281 gttgccgatt tggtcgatga cctgttcgat cttcatggga tcctttacgg gaacggggga
     2341 cgggtacaga gtacccgatc agcgagccgt aatcagcccc tcgagctccg atatggcctg
     2401 ctcgaggttg tcgttgacga tgatgtggtc gaacagctca ggcgcctcgg ccatctccga
     2461 ttgggcgatc tcgaggcggt cctcgatgtc ctcatccgac gtgtcgccac gacggcgcag
     2521 tcgcctctcc aactcgtcga ggctcggcgg agcgatgaaa tacatgatcg cttcaggctt
     2581 tcgctctcgc acctggcgag ccccctggat ctcgatctcg agcaggatgt cgcgttgatc
     2641 ggccaaagtc tcctcgacta gggcagctgg cgtcccgtag tagcggttgt tgtagacggc
     2701 ccactcgaga agctcgtcgt tctcgatcat gcgttcgaag tcccgccggg agatgaagtt
     2761 gtagtgcacg ccgtgcctct cgccagggcg gggcagcctc gtggtcgcgg acacagagaa
     2821 ctcgatgccg aggcgttcga tcagctctcg aacgatgctc gacttacccg agcccgacgg
     2881 cccggagacg acgagaagcc gggcggcgac cacctcagga gaactcggct aggagagcct
     2941 ctttctgctt gtctcccaga cccctgatcc gtcggttctc ggcgataccc agcgattcca
     3001 tcaagcgctt cgccttgatc ttccctgtgc caggcatcga tgcgatcacg cgctcgacct
     3061 tcagcccggc gaggatgtca tcgctgtcgg cccgctcgat caactcggag agacggagtg
     3121 aacctgtctt caagagctcc ttggcctcgg cgcgcaccct tctcgcctcc gctgccttgg
     3181 ccagcgcggc tgttctctgc tcatcggtca attggggtgg ttgggccatg tcctcatctc
     3241 gctcgccgaa cgaagaccct agccagcgtt gccggcggcg gcggccgaaa ccgcggccgt
     3301 gatctccttc gagaccgcct ctgccgcctc aatcggatcg gtcgcagccg tgatcgggcg
     3361 gcctacgacg agcaggtccg cccctgctct gatcgcctct cccggggtgg ccacccgtcg
     3421 ctggtcggcc ggttccgatc cggccggtct gatgcccgga gtggccacga tc
//
LOCUS       DASTXO010000172         3929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1336932, whole genome shotgun sequence.
ACCESSION   DASTXO010000172 DASTXO010000000
VERSION     DASTXO010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336932"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..1371
                     /locus_tag="VFU96_03685"
     CDS             184..1371
                     /locus_tag="VFU96_03685"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5112392.1"
                     /translation="MKRYLRYLALISALALVLAACGDGEGGDTTTTAGEATTTAGETA
                     TTAGEETTTSAAGESTTTGGETAFTPVKACFVTDDAGVDDRSFNASGWQGVQDAAASG
                     AAQPDPILLESSSEADYQPHIETCLAEGAEHIVGSGFKLGPTIETFAAANPDVQFTLI
                     DAASNGPNVAGTEFQTDEAAFAAGYLAAGMSQTGIVATYGGVNIPTVSIFMDGYARGV
                     AHYNEVNGAEVQVLGWDPADPEAGVMTGVFDPADPIVRATCESQLDEGADIFLPVGGA
                     INLPCGTAIQDRGVDGALIGVDADAFDAMPPEFQDLWMVTILKGLAGAVTGSIEAHAS
                     GQPLGGSSYVGNLANDGVGLSDYHSWTDRVPPELDAEVQQILADIEAGTITWEKYVVG
                     AGG"
     gene            1542..2990
                     /locus_tag="VFU96_03690"
     CDS             1542..2990
                     /locus_tag="VFU96_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5112393.1"
                     /translation="MSVEAGEIVALLGENGAGKTTLMNILYGLYSPDEGEVVVDGTKL
                     QLSNPSASIAAGIGMVHQHFMLVPVFTVAENVILGLEPLKSLDWIDKRAAHDLVKEVS
                     DRYNLAVDPDAMVEDLPVGVQQRVEIIKVLVRDARYIIFDEPTAVLTPQEVEEFFEIV
                     RGLKRDGKGVVFISHKLSEALKIADRIVVLRDGKSVGEADPATATREHLAELMVGRPV
                     LLTVEKETANPGPVVLSVEQLAVLDDRAHLTVDGVSFEVRAREIVGIAGVQGNGQTEM
                     VESITGLRPSLAGTILLDGIDITDHSPREIHRSGVAHVPEDRQESGLVLDFTIAENMV
                     LDAYYDPPYSRGIRMDWDAATEASQRLVDEYDVRTTRVDVPVSNLSGGNQQKVIVARE
                     FDRDVRLVIASQPTRGVDVGSIEYIHAQIVEQRDMGAAVLIVSSELDEVMALSDRVLV
                     MFKGRIVAEFDPASATKGEIGLAMLGSGEAVE"
     gene            2983..>3929
                     /locus_tag="VFU96_03695"
     CDS             2983..>3929
                     /locus_tag="VFU96_03695"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5112394.1"
                     /translation="MSDLTTEPVGDAGPSSPNGEPPSGPPSTMDRITGVGEGAGRALL
                     VPALALLLALIVGAVIIMVTDVDAWRRMGEDPGGALGDMVTGVWAAYRELFQGAFGSV
                     RAISETLFTASPLILAGLAVAVGFRTGLFNIGARGQMFIGGLFALWVGLHVELPALLH
                     IPLALIAAIIGGGLWGGLAGFLKARTGAHEVITTIMLNFIAAFFVLYVLKTSLFQEQG
                     SNLPQSAPILESARLPTLFGAQYRINISFLIALAAVFFVEWLLFRSTIGFEFRAAGFS
                     PSASAYAGMNVAFLYTTVMFISGALAGLAGATMTLGLPP"
BASE COUNT          724 a         1215 c         1258 g          732 t
ORIGIN      
        1 ggtcgcatgg gcgatcttcg gggccaacgt cgtgttcaag gcagtggtga ccgtgatttc
       61 gatcccctgg atctattggg ttcggcccga gccactggcg gcgacctaca cccaaaatgg
      121 tcgttaatgt gactcgtcgg gcagggggac cctgccattt catatcaatg gaggacaatc
      181 agcgtgaaac gctatttgcg gtatctcgcc ctcatctccg cccttgcact ggtgctcgca
      241 gcctgtgggg acggcgaagg cggagacacc accaccacgg ccggtgaggc cacgacgacc
      301 gccggagaga cggccaccac ggcgggcgag gagaccacga cgtctgccgc cggagagtcg
      361 acgacaacgg gcggggagac ggccttcact cccgtcaagg cctgtttcgt caccgatgac
      421 gcaggtgtcg acgaccggag cttcaacgca tccggatggc agggggttca ggacgccgcg
      481 gcaagtggcg cggcccaacc cgaccccatc ctgttggagt cgtcgagtga agccgactat
      541 cagccccata tcgagacctg tctcgccgaa ggagccgagc acatcgttgg ttccggcttc
      601 aagctcggtc ccacgatcga gacctttgcc gcggccaacc ccgacgtcca gttcactctg
      661 atcgacgccg cctcgaacgg gcccaatgtc gccggaaccg agttccagac cgatgaagcc
      721 gcgttcgcag ccggctatct ggccgccgga atgtcccaga caggcatcgt cgccacctat
      781 ggaggcgtca acatcccaac tgtgtccatc ttcatggatg ggtatgcacg tggtgtcgcc
      841 cattacaacg aagtgaatgg cgccgaagtg caggttctgg gatgggatcc agcggatccc
      901 gaggcggggg tcatgaccgg tgtcttcgac ccagccgacc cgatcgtgcg cgctacctgt
      961 gagagtcagc tcgacgaggg cgccgacatc ttcctcccgg tgggtggggc gatcaacctt
     1021 ccatgcggaa ccgcgattca agatcgcggg gtcgacggtg ctctcatcgg ggtcgacgcc
     1081 gacgccttcg atgccatgcc gcccgagttc caggacctct ggatggtcac catcctcaag
     1141 ggcttggcgg gcgcggtcac cgggtccatc gaagcccatg catccggcca gcctctgggc
     1201 ggatccagct atgtcggcaa ccttgccaac gatggagtcg ggctctccga ctaccacagt
     1261 tggacggatc gggtccctcc ggagctcgac gccgaggtcc agcagatact cgcggacatc
     1321 gaggccggga cgatcacctg ggagaagtac gtcgtcggcg ccggcggctg atccgaacga
     1381 cgaacagcta gaaaacgaga agcccccgcg tcctgcgggg gcttctcgta tctatagtgc
     1441 cgcaccggag gcggtcgact ggaaggagca cgctggtggc cctctagctg cgggggatta
     1501 cgaagagatt tcccactgtc gtcgccaacg acgacgtcag cgtgagcgtc gaagccggcg
     1561 agatagttgc ccttctcggg gaaaacggtg cgggcaagac caccctgatg aacatcctct
     1621 acggcctgta ctcgcccgac gaaggcgagg tcgtggtcga cggcaccaag ctccaactca
     1681 gcaatcccag cgcctcgatc gccgccggga tcggcatggt tcatcagcat ttcatgctgg
     1741 ttcccgtctt caccgtcgcc gagaatgtga tcctggggct cgagccgctc aagtcactcg
     1801 actggataga caaacgcgct gcccatgatc tggtcaaaga ggtctccgac cgatacaacc
     1861 tcgccgtcga tcccgatgcc atggtcgaag acctcccggt cggggtgcag caacgggttg
     1921 agataatcaa agtgttggtg cgcgatgcgc gatatatcat cttcgacgag cccactgcgg
     1981 tcctcacccc tcaagaagtc gaagagttct tcgagatcgt gcgcgggctc aagcgcgatg
     2041 gcaaaggggt tgtcttcatc agccacaagc tgagcgaggc tctcaagatc gccgaccgga
     2101 tcgtcgtcct cagagatggc aagtcggtgg gcgaagcaga cccggccaca gctacgaggg
     2161 agcacctggc agagttgatg gtgggacgcc cggtactcct cacagtcgag aaggaaacgg
     2221 caaaccctgg cccagtcgtt ctgtcggtcg agcagctggc ggtgctcgat gaccgtgccc
     2281 acctcaccgt cgacggcgtg tcgttcgagg tgagagctcg ggagatagtc gggattgcgg
     2341 gcgtccaagg caacggtcag acggaaatgg tggagagcat caccgggttg cgaccctcgc
     2401 tggcaggcac gattctgctc gacggcatcg acatcaccga tcacagccct cgtgagatcc
     2461 atcgaagtgg ggtggcgcat gtcccagagg atcgtcagga gagtggcctt gtcctcgact
     2521 tcaccattgc cgagaacatg gtgctcgacg cctattacga cccaccgtac tcgcggggga
     2581 tccgaatgga ctgggacgcg gcgaccgaag cctcccagcg gctggtcgac gagtatgacg
     2641 tccggacgac ccgggtggac gtgccggtct cgaacctctc cggaggcaat cagcagaagg
     2701 tgatcgttgc tcgggaattc gatcgcgacg tgcgactggt catcgcgtcg caaccaacca
     2761 ggggagtcga cgtcgggtcg atcgagtaca tccatgccca gatcgtggaa cagcgcgaca
     2821 tgggggctgc cgtgttgatc gtctcttccg agctcgatga ggtcatggcg ctgtcagacc
     2881 gggtgttggt gatgttcaaa ggccgaatcg ttgccgagtt cgacccggca agcgcgacca
     2941 aaggggagat aggtctggcc atgctcggct ccggtgaagc tgttgagtga cctgaccacc
     3001 gaaccggtcg gcgacgcggg gccgagttcg ccgaacggag agccgcccag cgggccgccg
     3061 agcacgatgg accggatcac cggcgtcggc gagggcgcgg gacgggcgct tctcgtcccg
     3121 gccctggctc ttttgctcgc gttgatcgtc ggcgcggtga tcatcatggt gaccgatgtc
     3181 gatgcctggc gcaggatggg agaggatccc ggcggagcgc tcggcgacat ggtcaccggt
     3241 gtttgggccg catatcggga gctgttccag ggggcgttcg gctcggtacg agcaatctcc
     3301 gagacactgt tcaccgctag tccgctgatc ctggccgggt tggccgtagc agtcgggttc
     3361 cgcaccggcc tgttcaacat cggagcacgc gggcagatgt tcatcggtgg gcttttcgcc
     3421 ctgtgggtcg gcctccacgt cgagctgccg gcattactcc acatcccact cgctctgatc
     3481 gccgccatca tcggtggtgg cctgtggggc ggcttggcgg gattcctcaa ggcaaggacc
     3541 ggcgcccacg aagtgatcac cacgatcatg ctcaacttca tcgccgcttt cttcgtgttg
     3601 tatgtgctga aaacgtctct gttccaggag caaggatcca acctgcccca gtcggctccc
     3661 atcctggagt cggcccggct gcccaccttg ttcggggcgc aatatcgaat caacatctcg
     3721 ttcctcatcg ccctggctgc ggtcttcttc gtggaatggc ttttgttccg gtcgacgatc
     3781 ggcttcgaat tccgcgccgc cggtttctcg cccagcgcca gcgcctatgc cggaatgaac
     3841 gttgcgttcc tctacacgac ggtgatgttc atctctggag ccctcgccgg gctcgcggga
     3901 gcgaccatga ctttggggct gcctccgta
//
LOCUS       DASTXO010000173         2290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_820797, whole genome shotgun sequence.
ACCESSION   DASTXO010000173 DASTXO010000000
VERSION     DASTXO010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820797"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..1932
                     /locus_tag="VFU96_03700"
     CDS             229..1932
                     /locus_tag="VFU96_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112395.1"
                     /translation="MNGDQDPSREGLPDPTLVARKGMVEWYHPTQLLLTALQVMVWDF
                     VGQGRSMEESPRSWKIHRFGRREVWFDFAADVGDGWNPAFAIASLIALPSLEAAGRPL
                     RHGDFLLLGGDQVYPVPTQSEYRDRFVEPYTQALRDSPGSRALLAIPGNHDWYDGLVS
                     FSRQFTQGRQIGNWHTIQEQSYFALKLPHRWWLWALDVRTGSHMDFGQRSYFYAAARK
                     LKPGDRVILVAAEPDWVTRDPRDREESHYLTVERDFIAPRKAAVHLWLAGDRHHYRRH
                     ELRDLDGTTDRNFQRITSGGGGAFLFPTHRPVRSNVVVRRCVARDGKPEVEEHEFVQK
                     TRFPSGVTSFRLSWLNLLFAVKNWKLGLFPMGVLYWLLTWVTLPPDWPAPSSWRIFLG
                     SPGMLLWLLTILFFFGVFADREKAWFRWIGGLAHGLAHVITAIGVTGIVNASFLAGAA
                     SLTDTLAAHVLNFLAGVVFGPTVFGLYLLLSMNLFGFHSLEAFSSLRIQDYKHFLRLH
                     IDRHGRLEIFPLAIHKAPRVGVGRGRYMLIEEPIVIDPHRREEKKAERVKPAVNLDSR
                     A"
     gene            complement(1929..>2290)
                     /locus_tag="VFU96_03705"
     CDS             complement(1929..>2290)
                     /locus_tag="VFU96_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112396.1"
                     /translation="FGDPRMGKPAYASLRALQHLALILRHQVLVDEKGAPYVTAKGLA
                     NLEMLKLRLCLMSGQLNAIFPKRGIETTQAYLRYHKLPRLHEPIKLPSYAHMDCFIGA
                     HASVDVFEEILGKLRQP"
BASE COUNT          380 a          753 c          719 g          438 t
ORIGIN      
        1 gcctcgccgc cgtagtaaag gacgaggtat gaaagtatcg ccgcgacgag accgatccag
       61 aggagccgcg gccacgggat ctttccttct ctgtcgacga ccaagtacgg gtaccaccac
      121 ttgtccgttc ggtccgccat cgcgaggatc cctcccgtcg gccgacgcgt tcaggtcccg
      181 cactacccgc cggaatacta gggcggcccg ctggaccgag tcaaatcagt gaacggcgac
      241 caggatcctt ctcgtgaggg gctcccggat cccactctgg tcgcccgcaa aggaatggtc
      301 gagtggtatc acccgacgca actgctcctc acggcactcc aggtcatggt gtgggatttc
      361 gtcgggcagg gccgctcgat ggaggagtcg ccccggtctt ggaagatcca tcgattcggg
      421 cgtcgtgagg tctggttcga tttcgccgcc gacgtcggcg acggctggaa tccggcattc
      481 gcgatcgcct cgctgatcgc gctgccgagt ctcgaggccg ctgggcgccc gctgcgccat
      541 ggcgattttc tcctgctcgg cggcgaccag gtctatccgg ttccgacgca gagtgagtat
      601 cgtgaccgct tcgtcgagcc gtacacccag gcgctgcgtg actcgcccgg cagccgtgca
      661 ctcctcgcga tccccggaaa ccacgactgg tacgacgggc tcgtgagctt ctcccgccag
      721 ttcacccagg gccggcagat cggtaattgg cacacgatcc aggagcagag ctatttcgcg
      781 ctcaagctcc cgcaccggtg gtggctctgg gcgctcgatg ttcgcaccgg gagtcacatg
      841 gacttcggcc aaagatcata cttctacgcg gccgccagga agctcaagcc aggcgaccgg
      901 gtcatcctgg tcgccgccga gccagactgg gtgacgcgcg accccaggga ccgggaggag
      961 agccactacc tcaccgtcga gcgcgatttc atcgcacccc gcaaggctgc ggtgcatctc
     1021 tggcttgcgg gcgaccgcca tcactaccgc cgccacgagt tacgcgacct ggacggcacg
     1081 acggaccgaa acttccagcg gatcacgagt ggcggtggcg gcgcgttcct cttcccgacc
     1141 catcgtccgg tccgaagcaa cgtcgtcgtc aggcgctgcg tcgcccgcga cgggaaaccc
     1201 gaggtcgagg agcacgagtt cgtgcagaag accagattcc cgagcggcgt gacgtccttc
     1261 cgtctgagct ggctcaacct cctgttcgcc gtcaagaact ggaagctcgg cctcttcccc
     1321 atgggagtgc tctactggct cctcacctgg gtcacgctcc cgcccgactg gccggccccg
     1381 agcagctggc gcatattcct cggctctccg ggaatgctgc tgtggctgct cacgatcctg
     1441 ttcttcttcg gcgtcttcgc cgaccgcgag aaagcgtggt tccgatggat cggcggtctg
     1501 gcccatggcc tcgcccatgt gatcacggcg atcggtgtca ccggcatcgt caatgcgagc
     1561 ttcttggccg gagcggcctc actcaccgac accctggcgg cgcacgtcct gaattttctc
     1621 gccggggtgg ttttcgggcc gacggtcttc ggcctgtacc tgctgctctc gatgaacctg
     1681 ttcggcttcc attcgctgga agccttctcg tcgctgagga ttcaggacta caagcacttc
     1741 ctgcgcttgc acatcgaccg tcacggccgc ctcgagatct ttccgctcgc gatccacaag
     1801 gcgcctcgcg tcggtgtggg ccgcgggcgc tatatgttga tcgaggagcc gatcgtgatc
     1861 gacccgcacc gccgcgagga gaagaaggcc gagagagtga agcccgccgt caatctggac
     1921 tcgcgcgcct agggctgacg aagcttcccg agaatctcct cgaagacatc gaccgaggcg
     1981 tgcgcgccga tgaagcagtc catgtgggcg tagctcggca gtttgatcgg ctcgtgcaga
     2041 cgcgggagct tgtgataccg gaggtaggcc tgcgtggtct cgattcctct cttggggaag
     2101 atggcgttca gctgccccga catcaggcag agccggagtt tgagcatctc gaggttcgcg
     2161 agacccttcg cggtcacata cggggcgcct ttctcgtcga ccagcacttg atgtcgcagg
     2221 attagcgcca ggtgttggag tgctcgaagg ctggcgtagg caggcttgcc cattcgtggg
     2281 tcaccgaaca
//
LOCUS       DASTXO010000174         2582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1557781, whole genome shotgun sequence.
ACCESSION   DASTXO010000174 DASTXO010000000
VERSION     DASTXO010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557781"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VFU96_03710"
     CDS             complement(<1..252)
                     /locus_tag="VFU96_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5112397.1"
                     /translation="MATRTEANTVKVAAVIQGVALVTFPAASTIFTSPDEYDVSNTQY
                     GAMFIPQAITAISASLAGAALSRRLGTKKLYVFGLLANLV"
     gene            complement(269..1822)
                     /locus_tag="VFU96_03715"
     CDS             complement(269..1822)
                     /locus_tag="VFU96_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEU5112398.1"
                     /translation="MADRYDFIIIGTGAGGGTLVNTLAASGKRILLLERGHFLQREMT
                     NWDPGEVFIEGRYISPDTWFDTDGKPFQPQVHYFVGGATKLYGAALYRFRPQDFGDLQ
                     HVDGVSPAWPVGYDDFEPYYTKAEWLYQVHGNHGEDPSEGAWSKQYPWPAVSHEPRIQ
                     QLHDDLVAGGYQPFHAPCGILLDEAERDMSTCIRCTWCDGYPCLVHAKSDAEVMAVRP
                     ALFNPNVTLLTGAEVTRLETDDSGRRVTRVVVSRDGSQEEYEADVVVLSAGAVNSAKI
                     LLASANDRHPNGLANGSDQVGRNYMFHNSKAVVAIGKERNDTLFQKTLGLNDFYLGSD
                     DYQYPMGNIQMVGKSNAEAMKGEEPMLTKLAPHWSLDEVAHHVVDFWLTTEDLPHPDS
                     RVTLDDDGNVVLNYHRTNDDEADHLYEELKRLLNHVGMADHHVLGKNFYMHMSIPIAG
                     VAHQAGTVRFGHDPATSVLDEFCKAHELDNLYVVDTSFFPSISAVNPALTAMANAIRV
                     GEHLVERVG"
     gene            1951..>2582
                     /locus_tag="VFU96_03720"
     CDS             1951..>2582
                     /locus_tag="VFU96_03720"
                     /inference="COORDINATES: protein motif:HMM:NF039283.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HEU5112399.1"
                     /translation="MSRFPPIAEYAFLSDCEADALVAADGSVEWLCLPRPDSPSVFGA
                     LLDRSAGSFRVGPSHTFVPDQRRYLPGTNIVETTWHTPSGWLTVYDLLVIAPTKDRPR
                     DESRPRAPADESATGTLLRIATCFAGRVEVQIDCFPLFDYGRQEGQWAYTGEGYHGAR
                     VTGGGLTIDVTSDARLDFTGPRAFGQTTLAEGESTFVALSWAGEAPADRA"
BASE COUNT          434 a          804 c          824 g          520 t
ORIGIN      
        1 caccaggttg gccaagaggc cgaagacgta cagcttcttc gtgccaagac gacgtgagag
       61 ggcggcaccg gccaacgatg ccgagatggc cgtgatcgcc tgcgggatga acatggcgcc
      121 gtattgggtg ttcgacacgt cgtactcgtc gggagaggtg aagatggtgc tggcggcggg
      181 gaacgtcacc agagcgactc cctgtatcac cgcggccacc ttgacggtgt tggcctcggt
      241 gcgtgttgcc atggctgatc cgacccccct aaccgactcg ttcgacgagg tgctccccga
      301 cgcggatcgc attggccatc gcggtcaacg ccgggttcac cgccgagata ctcgggaaga
      361 agctcgtgtc gaccacatag aggttgtcga gctcgtgtgc cttgcagaac tcgtcgagca
      421 ccgatgtggc cgggtcgtgc ccgaagcgaa cggttccggc ctggtgggcc acgccggcga
      481 tgggtatcga catgtgcatg tagaagttct tcccgagcac gtggtggtcg gccataccga
      541 cgtggttgag gaggcgtttc agctcctcgt agagatggtc ggcctcatcg tcgttggtgc
      601 ggtggtagtt caagacgacg ttgccgtcat cgtcaagggt caccctgcta tcggggtggg
      661 gaaggtcctc tgtcgtcagc cagaagtcga ctacatgatg ggccacctcg tcgagcgacc
      721 aatggggcgc cagcttcgtc agcatgggct cctccccctt catcgcctcg gcgttcgatt
      781 tgccgaccat ctggatgttt cccatcgggt actggtagtc gtcgctgccg aggtagaagt
      841 cattgaggcc gagggtcttc tgaaacaggg tgtcgttgcg ctccttgccg atcgccacga
      901 ctgccttgct gttgtggaac atgtagttgc ggcccacctg atcggagccg ttggccagac
      961 cattgggatg ccggtcgttg gccgaggcga ggaggatctt cgccgagttg acggctccgg
     1021 cggacagcac cactacatcg gcctcgtact cctcctggct cccgtcgcgg gagacgacca
     1081 cccgggtgac cctgcgcccc gagtcgtccg tctccagccg agtcacctcc gctccggtca
     1141 gcaaagtgac gttgggattg aacaaggccg gccgcaccgc catgacttcg gcgtccgact
     1201 tggcgtgcac caagcacgga tagccgtcgc accacgtgca cctgatacaa gtgctcatgt
     1261 cccgctccgc ctcgtcgagg aggatgccgc acggggcgtg gaacggctga tagccccctg
     1321 ctacgaggtc atcgtgcagc tgttggatcc ggggctcgtg cgagacggcg ggccagggat
     1381 actgcttgct ccatgctccc tcgcttgggt cttcaccgtg attgccatgt acctggtaga
     1441 gccactccgc cttggtgtag tacggctcga agtcgtcgta gcccaccggc caggccggcg
     1501 agacaccatc gacatgctga aggtcgccga agtcctgggg ccggaatcgg tagagggccg
     1561 cgccgtagag cttggtcgcg cccccgacga agtaatggac ttggggttga aacggcttgc
     1621 cgtcggtgtc gaaccaggtg tcgggggaaa tgtatcgccc ctcgatgaac acctcccccg
     1681 ggtcccagtt ggtcatctcc ctctggagga aatgtccccg ctcgaggagc agaatgcgct
     1741 tgcccgaggc ggcaagcgtg ttcaccagcg tgccgccacc cgctccggtc ccgatgatga
     1801 tgaaatcgta tcgatcagcc atctggtgct tctcccgatg atttccgccg gtcaatccga
     1861 tgttgtttct gaacggggca ggattcatcc cccagaacgg gtgatgcaac tggtcgattt
     1921 ctcctgcaga tttggacctg gaggtacgcg atgagcagat tccccccgat cgccgaatac
     1981 gcattcctgt ccgattgcga ggccgacgcc ctggtggcag cggatggctc cgttgagtgg
     2041 ttgtgtcttc cgcgaccaga ttcgccgagt gtcttcggtg ccctcctcga ccggtccgcc
     2101 ggcagttttc gcgtgggacc aagccacacc tttgtccctg atcaacgtcg ttacctgccg
     2161 gggaccaaca tcgtggagac gacctggcat accccatcag gctggttgac ggtctatgac
     2221 ctcctggtca ttgctcccac caaggatcgg ccccgtgacg agagccgtcc acgagccccc
     2281 gccgacgagt cggccacggg gactctcctc aggatcgcaa cttgcttcgc cggtcgtgtc
     2341 gaagtgcaga tcgactgctt ccctctcttc gactacggac gtcaagaggg ccagtgggcg
     2401 tacaccggcg aggggtatca cggcgcccgg gtaacgggtg gcgggctcac gatcgacgtg
     2461 acctccgatg ctcgcctcga tttcaccggt ccccgagcat tcggacagac cacgcttgcc
     2521 gagggggagt cgactttcgt cgccttgtcc tgggccggcg aggctccggc cgatcgggcc
     2581 ga
//
LOCUS       DASTXO010000175         2214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_600249, whole genome shotgun sequence.
ACCESSION   DASTXO010000175 DASTXO010000000
VERSION     DASTXO010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600249"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..>2214)
                     /gene="mfd"
                     /locus_tag="VFU96_03725"
     CDS             complement(141..>2214)
                     /gene="mfd"
                     /locus_tag="VFU96_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU5112400.1"
                     /translation="AVLGEQEVAGRRRAHRRAGRARTEVGQQYKDLTEGDFVVHHQHG
                     IGRFEGLVSRTMAGIERDYLVIAYAGEDRLYVPVDQLAAVRRYTGGEIPRVSRMGGKD
                     WTDQKAKVRKAVAAVAQQVVELHRVRAAATGHPYEPDTPWQREMEGSFPYEETHDQMQ
                     AIVDVKRDMEAGQPMDRLVFGDVGYGKTEVAIRAAFKAVQAGKQVAVLAPTTLLAQQH
                     HQTFAERFQSYPVRVEALSRFLTPKQQKTVVAGIGTGEVDVVIGTHRLLSEDIHFKDL
                     GLLVVDEEQRFGVSAKDAIKRLKVGVDVLTLTATPIPRTLEMALTGIRDVSHIRTPPE
                     DRHPILTYVGPYDEQSVSAAIRREILREGQVFYVHNRVQSIERAVARLQEMVPDARYV
                     VAHGQMSEGQLEQVMIDFWGGEYDVMVATTIIESGLDLPKVNTLIVERADLLGLAQLY
                     QLRGRVGRSDQRAYAYLFHPADQSLTEDAHRRLEAIGEATDLGSGFQLALRDLEIRGA
                     GSILGEVQSGHIAAVGFDLYAELVAEAVSELEGREPVTTAQREVRIDLPVDAHLPDAY
                     VADQELRLEAYRRLATTTTNDQVDDVAAEWVDRYGPLPTEAEALISLARLRVEALRVG
                     LEELVQLRHEIRMGPVDLKSSQEVRLRRLQPRSVLNAQEGVIFIPAPPDLVPGLVKFL
                     REMWAEEA"
BASE COUNT          390 a          726 c          701 g          397 t
ORIGIN      
        1 caccgctgcc tccgctgccg cacgcggcga gggctagtgc agcaatgatg aggagaattg
       61 catgcttctt caagacggaa tccttcgaga gaggggagat cagaagggta ctcggtgcca
      121 cgtggtagga ggacccacgc ctatgcctcc tcagcccaca tttcacggag gaacttgacg
      181 agaccaggga cgagatcggg gggcgcgggg atgaagatca caccctcctg agcgttcaag
      241 accgagcgcg gctggagtcg cctcagccgc acctcctgcg agctcttcag gtcgacaggg
      301 cccatcctga tctcatgacg caactggacc aactcctcca gaccgacgcg taatgcctcc
      361 acccggagtc gggcgagaga gatgagcgcc tctgcctccg tcggcagggg cccatatcgg
      421 tcgacccact cggccgccac gtcatcgacc tggtcgttgg tggtggtggt cgccaatcgt
      481 cggtaggcct cgagcctcag ctcctggtcg gcaacatagg catcgggcag atgggcgtcg
      541 acaggaagat cgatccggac ctcccgctgc gccgtggtca ccggctcccg cccctccagc
      601 tccgacaccg cctcggccac cagctccgcg tacagatcga agcccaccgc ggcaatgtgt
      661 ccactctgga cttctcccag gatcgagccc gcgcccctga tctcgagatc gcggagggcg
      721 agctggaagc cagaacccag atccgttgcc tccccgatgg cctccagccg ccgatgggcg
      781 tcctcggtca gagactggtc agctgggtga aagagatagg cgtaggcacg ctgatccgac
      841 cgtcccaccc ggccacgcag ttgatagagc tgggcaagac cgaggagatc ggcccgttcg
      901 acgatgaggg tgttcacctt gggcaggtcg agaccggact cgatgatggt ggtggcgacc
      961 atgacgtcgt attcgccgcc ccagaagtcg atcatcacct gctccagctg gccctcggac
     1021 atctgcccgt gggctaccac gtacctggcg tcgggaacca tctcttgaag ccgagccacc
     1081 gcgcgctcga tcgactggac cctgttgtgg acgtagaaga cctgcccttc gcggagaatc
     1141 tctcgtcgaa tcgcagccga gaccgactgc tcgtcgtagg gcccgacgta cgtcaagatc
     1201 ggatgacggt cttccggagg cgtcctgatg tgggagacgt cacgtatgcc ggtcagcgcc
     1261 atctccagcg tcctggggat cggggtggcg gtgagggtga ggacatccac accgactttg
     1321 agcctcttga tggcgtcctt cgcagaaaca ccgaaacgct gctcctcgtc gacgacgaga
     1381 agcccgaggt ccttgaaatg gatgtcctcg gacagcaggc ggtgggtacc gatcacgaca
     1441 tcgacttcgc ccgtgccgat accggcgacg acggtcttct gctgtttcgg tgtgagaaag
     1501 cgggacaggg cctcgaccct caccgggtag ctctgaaatc tctcggcgaa tgtctggtgg
     1561 tgctgctggg ccaggagtgt ggtgggagcg agcaccgcga cctgcttccc ggcctggacc
     1621 gctttgaatg cggctctgat cgcaacctcg gtcttgccat agccgacatc accgaagacg
     1681 aggcgatcca tcggttgccc ggcctccatg tcccgcttga cgtcgacgat ggcttgcatc
     1741 tggtcgtgcg tctcctcgta ggggaatgat ccctccatct cccgctgcca gggggtgtca
     1801 ggctcataag ggtggccggt ggcggcggct cgcacgcggt ggagctcgac gacctgctga
     1861 gcgacggcgg ccacggcctt gcgaactttg gccttctggt cggtccagtc cttccccccc
     1921 attcgggaga cgcgggggat ctcgcccccg gtgtaacggc ggaccgcggc gagctgatcc
     1981 acgggcacat agagccggtc ctctccggcg taggcgatca ccagatagtc acgctcgatt
     2041 ccagccatcg ttcgcgagac caagccttcg aaccggccga tgccatgctg atgatggacg
     2101 acgaagtcgc cttccgtcag gtccttgtac tgctggccta cctcggttcg ggcccgaccc
     2161 gcccgacgat gggcccggcg gcgcccggcg acctcctgct ctcccagtac ggcc
//
LOCUS       DASTXO010000176         6229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_379235, whole genome shotgun sequence.
ACCESSION   DASTXO010000176 DASTXO010000000
VERSION     DASTXO010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6229
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379235"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VFU96_03730"
     CDS             complement(<1..743)
                     /locus_tag="VFU96_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEU5112401.1"
                     /translation="MTTPRTLFQKVWDEHIVIDSDPTLLYVDLHLVHEVTSPQAFAGL
                     NQSGRTVRRPDLTVATVDHGIPTTGRSLGIRDPLSKKQIDALVQNCKEHDITMFGLDD
                     PRQGIVHVVGPEQGLTQPGLLVVCGDSHTSTHGAFGALAFGIGTTEVEHVLATQTIWQ
                     AKPQSMAVEVEGDLPWGVAAKDLILGIIAKIGVSGGTRHVIEYRGDAIRALSMEGRMT
                     ICNMSIEGGARAGLVAPDDTTFAYVEGRP"
     gene            complement(740..1819)
                     /locus_tag="VFU96_03735"
     CDS             complement(740..1819)
                     /locus_tag="VFU96_03735"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008871628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEU5112402.1"
                     /translation="MKSFSIAKVPGDGTGPEVVAEAVKVLDVAGKVHGFEIEYDELDL
                     GGERYLRTNETLTGADLQALGNADAILFGAVGHPKVPPGVLEQDILLRMRRELDQFIN
                     LRPVKLHPGVNGPLRDVKPEDVDFVVVRENSEGLYAGQGEFTAKGSPFEVAVQQSVNS
                     RFAVERCIRYAFDLAETRPRRHLTLCGKTNVLTYAWDLWSRVFDEVGAEYPHVQTSYA
                     HVDATCLWMVEDPGRFDVIVTDNMFGDIITDLAAAIQGGLGIAAGANINPNGVSMFEP
                     IGGTAPGFEGTGLINPLAAIGAAGMMLHDFGETAAATAIENAVGEVAGSLPSLRAGEM
                     GISTSEVGDRVASLVAEPEKAGTPA"
     gene            complement(1816..3012)
                     /locus_tag="VFU96_03740"
     CDS             complement(1816..3012)
                     /locus_tag="VFU96_03740"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEU5112403.1"
                     /translation="MEDRVRIFDSTLRDGEQAPGIALDPDQKVAIAAQLARLGVDIIE
                     AGFPISSPGDFKAVTRIAQEVQGPTIAALARAQAEDVERAWEAIQHASDPRIHVFLAT
                     SPIHMQHKLRMNEEEVITTVKANVGRARDHTENVEYSPEDATRSDPDFVIAVCQAAVE
                     AGATTVNIPDTVGYATPNDYGVLIRRVVDEVKAGREDVVVSTHCHNDLGLAVANSLAA
                     VEAGARQVEGAINGIGERAGNTSIEEVVMALLVRADAYGVGVGVTTEEIYETSRLVSE
                     QTGYPIQFNKAVVGRNAFAHESGIHQHGVLRERTTYEIMDPMTVGMGSSVIVIGKHSG
                     RAAFRHSLDQLGIEVNDAVFETSFAKMKEVADRTGEVTPEQIRAIVEEVVSGIEVLDG
                     VTASFE"
     gene            complement(3139..3648)
                     /gene="ilvN"
                     /locus_tag="VFU96_03745"
     CDS             complement(3139..3648)
                     /gene="ilvN"
                     /locus_tag="VFU96_03745"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018350786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEU5112404.1"
                     /translation="MQHTLTVVVEDKPGVLARISAMFSNRGFNIHSLAVGPTHQDGRS
                     RITLVVDAPELEQLKKQLNKLVNVIKVQEMEPAEALEREVMIVRISAQPATRGEVRDT
                     ASLFDARVIDISSSAMTFEVSGTPQHLRTFIEHLGPYGVIDLVKSGRIAMKRESELSP
                     APVAAGVGK"
     gene            complement(3648..5369)
                     /gene="ilvB"
                     /locus_tag="VFU96_03750"
     CDS             complement(3648..5369)
                     /gene="ilvB"
                     /locus_tag="VFU96_03750"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEU5112405.1"
                     /translation="MTQPSNFTGSHSLLRSLEHEGVEIIFGVPGGAILPAYDPLLDSP
                     IRHVLARHEQGAGHMAEGYAQATGLVGVVMATSGPGATNLVTPLQNALMDSTPIVAIT
                     GQVPTSAIGSDAFQEAYTTGLAMHCTKHSYMATNADDIPNLIHEAFHLASTGRPGPVL
                     VDIPKDVLNQVTTWREPHLHNLPGYKPTVSGHPMQIRAALDMIEQAERPVLYVGGGVI
                     KAGAASELLAFAEAVNAPVVTTLMARGAFPDDHPLALGMPGMHGLYTATTAIQKSDVL
                     ISLGARFDDRVTGAPDAFAPNAKIIHVDVDPAEIGKVRAADVPIVGDARSVLTDLLSG
                     LSARRDPADAPVREEWFAQLGQWQEKYPLAYEQDPDGPIKVQHFIERLYSITGGDAVI
                     AAGVGQHQMWASQFWGFREPRSWVNSGGLGTMGFAVPAAVGAKAARPDDLVIALDGDG
                     CFQMTCQELITATTEEIPVKIVVFNNGGYGMVKQWQNLFYGGRLSAVDLGFTYPDYPK
                     LAEAMGCVGLKVEHPSDIDNAIEKALSVNDRPVVLEVVVDGEEMCFPMVPAGGSNDRV
                     VMRREEL"
     gene            5581..>6229
                     /locus_tag="VFU96_03755"
     CDS             5581..>6229
                     /locus_tag="VFU96_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU5112406.1"
                     /translation="MSRYGTVELGGTWTSCAVGSSPDHLSRSVKFSTSDPTETLRMVA
                     EFMGSETVDAVGVAAFGPLDLERGRVGVTPKEGWSGIDLLGPLREALDAPIGLDTDVI
                     GAALGEGRWGAGRGFGTFVYVTVGTGIGGGALVDGQPLHGLGHPEMGHISVRRHPDDD
                     YRGVCMLHVDCLEGLASGPAIEQRLGRRGQDLMGTDLATAVELESFYLSQMMRDLV"
BASE COUNT         1059 a         1921 c         2046 g         1203 t
ORIGIN      
        1 aagggacgac cctcgacgta ggcgaaggtc gtgtcgtcgg gggcgacgag ccccgcgcgg
       61 gcgccaccct cgatcgacat gttgcagatg gtcatccggc cctccatcga cagggcacgg
      121 atcgcgtcgc cgcggtactc gatgacatgc cgggtcccgc cggatacgcc gatcttggcg
      181 atgatcccca ggatcaagtc cttggcggcg actccccagg gcaggtctcc ttcgacttcg
      241 accgccatcg actgcggctt ggcttgccag atggtctgag tggcgagcac gtgctcgacc
      301 tccgtagtgc cgatgccgaa ggcgagggcg ccgaacgcac catgggtcga ggtgtggctg
      361 tcaccacaga cgacgagcag gccgggctgc gtcaggccct gctcgggccc tacgacgtga
      421 acgatgccct ggcgcggatc gtcgagaccg aacatcgtga tgtcgtgttc cttgcagttc
      481 tgaacgagcg cgtcgatctg cttcttggac aggggatcgc ggatcccgag ggaccgcccg
      541 gtcgtcggga tgccgtggtc caccgtggcc acggtcaggt cgggtcgtct caccgtccgg
      601 cccgactgat tcagcccggc gaaggcctgg ggtgaggtga cctcatggac gaggtgaagg
      661 tcgacataca gcaaagtggg gtcggaatcg atcacgatgt gctcgtccca caccttctgg
      721 aagagcgtgc gcggagtggt caagcggggg tcccggcttt ctccggctcg gcgacgagtg
      781 atgcgacccg gtccccgacc tcggaggtgc ttatccccat ctctccggcg cggagggaag
      841 ggagcgatcc agcgacctca cccacggcgt tctcgattgc ggtggccgcc gccgtctcac
      901 caaagtcatg aagcatcatc ccggcggctc caatggcggc tagtgggttg atgagaccgg
      961 ttccctcgaa ccctggagcg gtgccgccga tcggctcgaa catggagact ccgttgggat
     1021 tgatattcgc cccggcggcg atgccgaggc cgccttggat ggcggctgcc aggtcggtga
     1081 tgatgtcgcc gaacatgttg tcggtgacga tgacatcgaa gcggcctgga tcctcgacca
     1141 tccacaggca cgtcgcatcg acatgagcgt atgaggtctg tacgtgcgga tactccgcgc
     1201 ccacctcatc gaacaccctc gaccagagat cccatgcgta cgtgaggaca ttggtcttgc
     1261 cgcagagagt gaggtgtcgt ctcggccggg tctcggccag atcgaacgca tagcggatgc
     1321 agcgttcgac cgcgaagcgg ctgttgaccg attgctggac tgcgacctcg aatggtgaac
     1381 ctttggccgt gaactcaccc tgacccgcgt acagcccctc actgttctcc ctgaccacga
     1441 cgaagtcgac gtcctcgggc ttcacatccc ggagtgggcc attgaccccg ggatggagct
     1501 tcaccggacg cagattgatg aactggtcca actcacggcg catcctcagc aggatgtcct
     1561 gctccagcac gccagggggc accttgggat gtccgacggc gccgaagaga atcgcatcgg
     1621 cgttgccgag ggcctggagg tctgccccgg tcagcgtttc attggttcgc agatatcgtt
     1681 caccgccgag gtcgagctcg tcgtattcga tctcgaatcc gtgcaccttc ccggcgacat
     1741 cgaggacctt gaccgcttcg gccaccacct ccggcccggt cccgtccccg gggaccttcg
     1801 ctatggagaa gctcttcatt cgaacgaggc cgtgaccccg tctagtacct cgatgccgga
     1861 cacgacctcc tcgacgatgg cccggatctg ctccggggtc acctcgccgg tgcgatcggc
     1921 cacctccttc atcttggcga acgacgtctc gaagacggcg tcgttcactt cgatgccgag
     1981 ctggtccagt gagtgcctga atgcagcgcg tccggagtgc ttcccgatca cgatcaccga
     2041 gctgcccatc ccaactgtca tcgggtccat tatctcgtat gtcgtgcgct ctctcagcac
     2101 accatgctgg tggatgcccg actcgtgggc gaaggcgttg cgcccaacca cggccttgtt
     2161 gaactggatc ggatagcccg tctgctcgga taccaacctc gaagtctcgt agatctcctc
     2221 ggtggtgact ccaacgccga cgccgtaggc gtcggcccgt accagcagcg ccatgacgac
     2281 ctcttcaatc gaggtgttcc cggcacgttc tccgatcccg ttgatggcgc cctcgacttg
     2341 gcgggcgccc gcctccacgg cagcgaggga attggccacc gccaggccga gatcgttgtg
     2401 acagtgtgtc gacacgacga cgtcttcgcg accggctttc acctcgtcga cgacccggcg
     2461 gatcaggacc ccgtagtcgt tgggggtggc gtacccgacc gtgtcgggga tgttgaccgt
     2521 ggtcgctcct gcctcgacgg ctgcctggca aacggcaatg acaaagtcag gatcggaacg
     2581 ggtggcgtcc tctggtgagt attcgacgtt ctcggtgtga tcgcgggcac ggccgacatt
     2641 cgccttgacg gtcgtgatca cctcttcctc gttcatcctg agcttgtgct gcatatggat
     2701 gggtgaggtt gccaggaaca cgtggatgcg cgggtcggac gcgtgttgga tcgcctccca
     2761 agcgcgttcg acgtcctcgg cctgggcacg ggccagggcg gcgatcgtcg ggccctgcac
     2821 ctcctgagcg atccgagtca cggccttgaa atctcctggc gaggagatgg ggaacccggc
     2881 ctcgatgatg tcgaccccca agcgggcgag ctgggcggcg atcgccactt tctggtcggg
     2941 gtcgagagcg atgccaggcg cctgttcacc gtctctgagt gtgctgtcga aaatccttac
     3001 tcggtcttcc atttgtctct cctgaatctc ggggtctcgt cggggcgccg gtctgcccgg
     3061 gtgggcgttc cggctagata agagcgaggc cgaagaggct caggagagcc aggagacaga
     3121 tgagcagtgt ggagcgggtc atttccccac cccagccgca accggggcgg gactcagctc
     3181 ggattcccgc ttcatggcga tccgacccga cttgaccaga tcgatcaccc catagggccc
     3241 gaggtgctcg atgaaggttc tcaggtgctg tggtgtgccc gacacttcga acgtcatggc
     3301 cgaggagctg atatcgatga ccctggcgtc gaagagagag gccgtgtccc ggacctcgcc
     3361 acgagttgcc ggctgggcgc tgatccggac gatcatcact tcccgctcca acgcctccgc
     3421 cggctccatc tcctggacct tgatcacatt gaccagcttg ttgagctgct tcttcagctg
     3481 ctcgagctcg ggggcatcga cgacgagcgt gatgcgggat cgcccgtcct gatgagtcgg
     3541 gccgaccgcc agggagtgga tgttgaagcc ccggttggag aacatggccg agatcctggc
     3601 caatactcca ggcttgtcct cgacgaccac ggtgagggtg tgctgcatca gagctcctcg
     3661 cgtcgcatga cgacacggtc gttggaacca ccggcaggga ccatcgggaa gcacatctcc
     3721 tcgccgtcga ccacgacctc gagcacgacg gggcgatcat tgactgacag cgccttttcg
     3781 atcgcgttgt cgatgtcgga cggatgctcg accttcagcc cgacacaacc catcgcctct
     3841 gcgagtttcg ggtagtcggg ataggtgaac ccgaggtcga ccgcggaaag tcgcccaccg
     3901 tagaagaggt tctgccactg cttgaccatc ccgtagccgc cgttgttgaa aacgacgatc
     3961 ttgacgggaa tctcttctgt cgtggcggtg atcaactcct ggcaggtcat ctggaaacag
     4021 ccgtcaccgt cgagggcgat gaccaggtcg tcgggtcgtg ccgccttggc cccgaccgcg
     4081 gccggaacgg cgaatcccat cgtccccagg ccaccggagt tgacccacga tctcggctcc
     4141 cggaagcccc agaactggga ggcccacatc tggtgctgac ccaccccggc ggcgatcacc
     4201 gcatcgcctc cggtgatgga atagagccgc tcgatgaaat gctggacctt gatcggccca
     4261 tcagggtcct gctcgtaggc gagtgggtac ttctcctgcc actgtccgag ctgagcgaac
     4321 cactcctctc gaacgggggc gtccgccggg tcgcgccggg cgctcaggcc gctgagcagg
     4381 tcggtgagca ccgaccgggc atctccgacg atgggcacat cggcggcccg caccttgccg
     4441 atctccgctg gatccacatc gacgtggatg atcttggcgt ttggagcaaa cgcgtccggc
     4501 gccccggtca cccggtcgtc gaatcgggct ccgaggctga tcagcacgtc tgatttctgg
     4561 atcgccgtcg tagcggtgta gagcccgtgc atgcccggca tcccgagggc gagcggatga
     4621 tcgtcgggga atgcaccccg ggccatgagc gtcgtgacga ctggtgcgtt gacagcttcg
     4681 gcgaaggcga gcagctcgga agcggcgccg gctttgatca ccccgccgcc gacatacaga
     4741 accggtcgct cagcctgctc gatcatgtcg agggcggcgc ggatctgcat cggatgcccc
     4801 gagacggttg gcttgtaacc ggggaggttg tgcagatgtg gttcccgcca ggttgtgacc
     4861 tggttgagga catccttggg aatgtcgacg aggacgggac ccggccgtcc ggtggatgcg
     4921 agatggaatg cctcgtggat caaattcggg atgtcgtcgg cattcgtcgc catgtatgag
     4981 tgcttggtgc aatgcatggc gagaccggtg gtgtaggcct cctggaatgc gtcacttccg
     5041 atggccgatg tcgggacctg cccggtgatg gcgacgatcg gtgtcgaatc catcagcgcg
     5101 ttctgcaaag gagtgaccag gttggtcgct ccgggcccgg aggtggccat caccacgcct
     5161 accagtccag tggcctgggc atagccctcg gccatgtgcc cggcgccctg ctcgtgccgg
     5221 gccagcacat gccttattgg actgtcaagg agggggtcgt aggcagggag aatggcacct
     5281 ccgggcacgc caaaaatgat ctcgacccct tcgtgctcga ggctacgaag gagcgaatgt
     5341 gatccggtga agttgcttgg ttgtgtcatg gtgtctggta acaaaaaacc ctccggggcg
     5401 gagggtcgag cgcacgcagg tctgtttgcg cgcgctacac gataagaagg agtacgtcgg
     5461 cgggggcgaa caactgtctc acgtgtggtt gccaagtaaa caacttgcct ttacgcatgt
     5521 caagaacatc gggtgttcgc gccgatccct gaatggatgg ttccgactta cagtcgcacc
     5581 atgtccaggt acgggacggt ggagttgggt gggacctgga cgtcgtgtgc ggttggatcc
     5641 tccccggatc acctgtcgag atcggtcaag ttctccacct ccgatccgac ggagacgctt
     5701 cggatggtgg ctgagttcat ggggagtgag acggtcgatg cggtaggtgt cgcagccttc
     5761 ggaccactgg acctcgagcg aggccgcgtt ggtgtgacac ccaaggaggg ctggtcgggg
     5821 atcgatctct tgggcccgtt gagggaagcg ctcgacgcgc cgatcggatt ggacacagac
     5881 gtgattggcg ccgccctcgg cgagggccga tggggcgccg gccgtgggtt tggcacgttt
     5941 gtgtatgtca ccgtgggcac cgggatcggt gggggtgctc ttgtcgacgg tcagccactt
     6001 cacggcctcg ggcatcccga gatgggtcat atctcggtcc gacggcaccc cgacgacgac
     6061 tatcgcggcg tctgcatgct ccacgtagat tgcctcgaag ggctcgccag cggcccggcc
     6121 atcgagcagc gcttggggag gcgggggcag gatctcatgg ggacggatct ggcgacggct
     6181 gtcgagctcg agtctttcta cctgagtcag atgatgcggg atctggtct
//
LOCUS       DASTXO010000177         2874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_637430, whole genome shotgun sequence.
ACCESSION   DASTXO010000177 DASTXO010000000
VERSION     DASTXO010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637430"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VFU96_03760"
     CDS             <1..551
                     /locus_tag="VFU96_03760"
                     /inference="COORDINATES: protein motif:HMM:NF015780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEU5112407.1"
                     /translation="PNWLSAEPGARQSIGAGADLVTFSGDKLVGGPQAGIIVGSTEVV
                     DRLRQNPLARALRIDAMTEAALAATLEAYAGGGADQIPIWHMVTLTEPDLAARVDDLA
                     SRVGGAARPGESVVGAGSVPGVGIPTPQIVLEGEDHLHGRLLAAPQPVLTRRVDKDLI
                     LDLRAVAEEDDDVIADMVAGCR"
     gene            542..2296
                     /gene="selB"
                     /locus_tag="VFU96_03765"
     CDS             542..2296
                     /gene="selB"
                     /locus_tag="VFU96_03765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEU5112408.1"
                     /translation="MPLIGTAGHVDHGKSTLVEALTGLDPDRWPEEKERGLTIDLGFA
                     WTTMEPVGEVSFVDVPGHERFLKNMLAGIEAIDVALFVVAADEGWMPQSEEHLAVLDL
                     LGIDSGVVALTKIDAVGPERADDAGREVARQLKGTTLHDAPILPVSARTGDGMERLRE
                     QLVARLSGVRHGGNRPRLWVDRAFSVRGAGTVVTGTLTEGPLSVGDDLMLLPVGKRTR
                     IRGMQSHEKEIDVAGPGRRLALNLTGLDRSETLRGTMLGLPGQWELSARFSARLRAAR
                     YVDDLTRRGAFQVHLGSGAHPATIKRLEGGYALIEVPTALPIRTGDRFILRESGRRLV
                     VAGGVVIDPAPGPPARAIRTAQVIDPLGEKDDVATALLALRGMDDVGRLAAHSGGGTP
                     RDSVLVGRTALTSEQFDDLRSQAETMVSAHHDQHPLRPGIPLATLATNLGISGELAER
                     LVSDSEEIERSGPDVSARHRHLSLDPDSRLRWDRARALLGEGLAVPHSEELGLGRELT
                     HLLIRDGLVVRVSDDLVYLPEQIGEIKHLLSEMTEPFTVADFRDRSGLSRKYAVPILE
                     WADREGLTIRRGDVRHLR"
     gene            complement(2319..2558)
                     /locus_tag="VFU96_03770"
     CDS             complement(2319..2558)
                     /locus_tag="VFU96_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020642884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEU5112409.1"
                     /translation="MDDWQLDALCRGSHAFLFFPPSTFERKDERERREMKAKAICGVC
                     PVRVPCTTHALQIREPFGIWGGLTEVERRSMPLVG"
     gene            complement(2648..>2874)
                     /locus_tag="VFU96_03775"
     CDS             complement(2648..>2874)
                     /locus_tag="VFU96_03775"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEU5112410.1"
                     /translation="FSTRTIGRDGLIGERGVAIDAFDPDGIVEVKGARWRATAHREAG
                     LVAGSEVLVAGVDGMFLEVEPAIGPAKTDH"
BASE COUNT          488 a          914 c          992 g          480 t
ORIGIN      
        1 ggcccaattg gctgtccgcc gagcccggtg cccgtcagtc gatcggggcg ggagcagatc
       61 tggtgacttt cagtggtgac aagctggtcg gtgggccgca ggcagggatc atcgtcggct
      121 caaccgaggt ggtcgaccgg ctccggcaga acccactggc gcgggcgctg aggatcgacg
      181 ccatgacgga agccgcgctg gcggcgacac tcgaggcgta cgccggcggg ggtgcggatc
      241 agatcccgat ctggcacatg gtcacgctca ccgagcccga tctcgcggcg agggtcgatg
      301 acctcgcttc ccgggtgggg ggcgcagccc gacccgggga atccgtcgtc ggggcgggaa
      361 gcgtgcctgg tgtgggcatc ccgacacccc agatcgtcct cgagggcgag gaccatctcc
      421 atggccgtct cctcgccgct ccgcaaccgg ttctgacccg gcgggtggac aaagacctca
      481 tcctcgacct gagggcggtg gccgaggaag acgacgacgt gatcgcggac atggtggcgg
      541 gttgccgctg atcggaactg cggggcatgt cgatcacggc aagtcgaccc tggtcgaggc
      601 gctcaccggg ctcgaccccg atcgatggcc ggaggagaag gaacgcgggc tgaccatcga
      661 cctggggttt gcctggacca ccatggagcc agtaggcgag gtttcgtttg tcgacgtgcc
      721 cggtcacgag cggtttctca agaacatgct ggccgggatc gaggcgatcg atgtcgccct
      781 attcgtcgtc gccgccgacg agggctggat gcctcaatcg gaggagcatc tggcggtgct
      841 cgacctcctc ggtatcgact cgggtgtcgt cgccctgacc aagatcgacg cagtgggtcc
      901 cgagcgagcc gacgatgccg ggcgtgaggt cgcgcgacag ctgaagggca ccacgctgca
      961 tgacgcgccg atcctcccgg tctcggccag gacaggcgac ggcatggagc gcctccgcga
     1021 gcagctggtc gcacggctgt ccggggtacg gcatgggggc aatcggccgc gtctctgggt
     1081 ggaccgtgcc ttttcggtgc gtggggcggg cacagtggtc acaggcacgt tgacggaggg
     1141 cccactgagt gtcggggacg atctcatgtt gctcccggtc ggcaagcgga cccgaattcg
     1201 ggggatgcag agccacgaaa aggagatcga cgtggctggc ccgggtcgac gactcgctct
     1261 gaatctgacc ggcttggatc gatcggagac cttgcggggg acgatgctgg gcctgcccgg
     1321 gcaatgggag ctgtcggcac ggttcagtgc gagactcagg gcggcacgat acgtcgatga
     1381 cctgacccgc agaggtgcat tccaggtcca tctcggcagc ggcgcccatc ccgcaacgat
     1441 caagcggctg gaggggggat atgccctcat cgaagtcccg acagcccttc ccatcagaac
     1501 cggggatcgt ttcatcctgc gcgaatccgg tcgccgtctg gtcgtggccg gaggcgtggt
     1561 catcgacccc gctccgggtc ccccggcccg agcgatcagg accgcacagg tcatcgaccc
     1621 tctcggcgag aaggacgatg tcgccacggc tctcctggcg ttgcgcggca tggacgacgt
     1681 cgggcgtctc gccgctcaca gcggcggcgg gactccccgg gactcggtcc tggtcggcag
     1741 gaccgcactg accagcgagc agttcgacga cctgcgttcg caggcggaga cgatggtgtc
     1801 tgcccatcat gaccagcacc cgctgcggcc ggggattccg ctggcgacgc tcgccacgaa
     1861 tctgggaatc tccggtgagc tggccgagcg actggtgagc gacagcgagg agatcgagcg
     1921 aagcgggcct gacgtgtcgg cccgacatcg tcatctgagc ctggacccgg attcccggtt
     1981 gagatgggac cgggccaggg cactgctggg agagggcctg gcggtgcctc actccgagga
     2041 gctcggcctg ggacgggaac tgacccatct cctgatcagg gacggcctgg tggtccgtgt
     2101 ctccgacgac ctcgtctatc tgcccgagca gataggtgag atcaaacacc tgttgagcga
     2161 gatgaccgag ccattcaccg tggcggattt tcgtgaccgc tctggtctgt cacgaaaata
     2221 cgcagttccc atactggaat gggcggacag agaagggctc accatccggc ggggcgatgt
     2281 gcgccacctt cggtgagccc tgactccggt gccggcggtc aaccgaccag cggcatgctc
     2341 cttctctcga cttcggtgag acctccccag ataccgaatg gttcccggat ctggagggcg
     2401 tgtgtggtgc agggaacccg cacgggacac accccgcaga tcgctttggc cttcatctct
     2461 ctgcgctccc gctcgtcttt gcgctcgaac gtcgaaggcg gaaagaaaag aaaggcatga
     2521 ctccctctgc agagggcgtc gagctgccaa tcgtccatgc ccgacaaacg gactcctccc
     2581 tgtctagcga caaccggcgc gaacctactc agggcgatgc ccggcggcaa ggaagaaaat
     2641 ggcgatttca gtgatctgtt ttcgcggggc cgatggcagg ctcgacctcg aggaacatcc
     2701 cgtcgacgcc tgcgacgagg acctccgagc ccgctaccag cccggcctca cggtgagcgg
     2761 tcgcccgcca ccgtgcgcct ttgacttcga caatgccgtc ggggtcgaac gcgtcgatcg
     2821 cgactcctct ttccccgatc agaccgtcgc gtccgatcgt cctcgtcgag aacc
//
LOCUS       DASTXO010000178         2002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_932086, whole genome shotgun sequence.
ACCESSION   DASTXO010000178 DASTXO010000000
VERSION     DASTXO010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932086"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VFU96_03780"
     CDS             <1..835
                     /locus_tag="VFU96_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015801234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU5112411.1"
                     /translation="LYTLFVLYPITQSIRYSGYDWNGLEPLTDWVGLQNFRDAFADPL
                     FIEALTHNGIIIGLSLLLQIPFAVALAVLLSRNLKGRGVFRTMYFAPFILSEVVTGVV
                     WRQIYRPDGLFDQMLGSAGAGDLTREWLADPKIALYSLFFVISWKYFGFHMVLILAGL
                     QLIPTELEEASAIDGASWWQSFRLVTLPLLGPTIRVSMFLSIIGALQLFDLVWVTTKG
                     GPVNSTATMATYLVDWGFRRSQFGYASAISVIVFGLSLIVAILYQRFVLRRDLEGALT
                     T"
     gene            832..1692
                     /locus_tag="VFU96_03785"
     CDS             832..1692
                     /locus_tag="VFU96_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020132799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU5112412.1"
                     /translation="MTAVTAPTSNSTRKYDFGRGLLYVIAVVVLLVIVVPLSFSIIGG
                     FRSNAQLAADPVALPNPWIFTNYQTALTDPDFYQWVRNSVLIALITTALVLPAASLAA
                     FVIARYRFKGREVIFALFTLGLLFPVAVAILPLFITLRQTGLLNSPLGIALPQAAFGL
                     PLSIVILRPFFRSIPRDLEDAAAIDGCGPLRFYWSVMLPLSRPVLSTVAVLTVVGSWN
                     AFLLPLLVLIQPEQWSLPLGINNISAQYVTDTAMILAYTTLAMIPALVFYFIAQRQIV
                     SGLTAGGVKG"
     gene            1689..>2002
                     /locus_tag="VFU96_03790"
     CDS             1689..>2002
                     /locus_tag="VFU96_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase"
                     /protein_id="HEU5112413.1"
                     /translation="MTAETVDGQLPAYRDPGLPVGERVESLLEMMTLEEKVAQLGSAW
                     VFQLTEDGRLSDDKARDVLGDGLGHVTRVSGASDLGAEDAARLANQIQRFLLDETRLG
                     IPA"
BASE COUNT          320 a          695 c          555 g          432 t
ORIGIN      
        1 cctctacacc ctcttcgtcc tctacccgat cacccagagc attcgctata gcgggtacga
       61 ctggaacggt ttggagccac tcaccgattg ggtgggcctg cagaacttca gagatgcttt
      121 cgccgaccct ctgttcatcg aggcgctgac ccacaacggg atcatcatcg ggctctctct
      181 cctgctccag atcccgttcg cagtcgccct cgccgtactt ctcagtcgca acctgaaagg
      241 gcgcggggtg ttccggacga tgtatttcgc tcctttcatc ctctccgagg tggtaaccgg
      301 tgtggtgtgg cgccagatct atcgtcccga cggcctcttc gaccaaatgc tcggctcggc
      361 cggtgccggc gacttgacca gggagtggct cgcagacccc aagatcgccc tctacagcct
      421 gttcttcgtg atctcctgga agtacttcgg cttccacatg gtgctgatcc tggccggtct
      481 ccagctcatc ccgaccgagc tcgaggaggc gtcggccatc gacggtgcat cgtggtggca
      541 gagcttccga cttgtcactc tgcccttgct cgggccaacc atcagggtct cgatgttcct
      601 ctccatcatc ggcgccctac agctcttcga tctggtctgg gtcaccacca aggggggccc
      661 ggtgaactcc accgccacca tggccaccta cctcgtggac tggggcttcc gcagatctca
      721 attcggatat gccagcgcca tctcggtgat cgtcttcggg ctctccttga tcgtggccat
      781 cctctaccaa cgattcgtct tgcggcgcga cctggaaggg gccctcacca catgacggcg
      841 gtcacagctc caaccagcaa ttcgacccgc aagtacgact tcggtcgcgg gctgctctac
      901 gtgatcgcgg tcgtggtcct tctggtgatc gtggtgcccc tgagcttctc gatcatcggc
      961 ggattccgga gcaacgccca gctcgccgcc gacccggttg cactccccaa tccctggatc
     1021 ttcacgaact accagaccgc tctcaccgac ccggacttct atcagtgggt gaggaacagc
     1081 gtgctgatcg cgctgatcac cactgccctg gttcttcccg cggcatccct ggcggcgttc
     1141 gtcatcgccc gctaccggtt caagggccgc gaggtcatct tcgctctgtt cacgctgggg
     1201 ctgttgtttc ccgtcgccgt cgccatcctg cccctgttca tcactctccg tcagaccggc
     1261 ctgctcaaca gccccttagg aatcgccctc ccccaggcgg cattcgggct cccgctatcg
     1321 atcgtgatcc ttcgtccctt cttccgcagc atcccccgtg acctcgaaga cgcagccgcc
     1381 atcgacggct gtgggcccct gcgcttctac tggtccgtga tgcttcccct gtcccgtccg
     1441 gtgttgagca cagtcgccgt gctcacggtg gttgggagct ggaacgcctt tctcctcccg
     1501 ttgctggtgt tgatccagcc ggagcagtgg agcctgccac tcggcatcaa caacatctct
     1561 gctcaatacg tgacagatac cgcgatgatc ctggcctaca ccacgctggc aatgatccct
     1621 gccctcgtct tctacttcat agcccagcgt caaatagtga gcggtctcac cgcaggagga
     1681 gtcaagggat gaccgcggag accgtcgacg gtcagctgcc ggcctaccga gacccgggtc
     1741 ttccggtcgg cgagcgagtc gagagtctgc tcgagatgat gactctcgag gagaaggtcg
     1801 cacaattggg gagcgcctgg gtcttccagc tgacagaaga cggacgcctc tccgacgaca
     1861 aggcccgtga cgtgctcggt gacggtctcg gccatgtgac ccgggtctct ggcgccagcg
     1921 acctcggggc ggaggatgcg gctcgcctgg caaaccagat ccagcgtttc ctcctcgacg
     1981 agacccgact cggcatcccg gc
//
LOCUS       DASTXO010000179         2286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1373905, whole genome shotgun sequence.
ACCESSION   DASTXO010000179 DASTXO010000000
VERSION     DASTXO010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373905"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VFU96_03795"
     CDS             <1..184
                     /locus_tag="VFU96_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate--acyl-ACP acyltransferase"
                     /protein_id="HEU5112414.1"
                     /translation="EVRRRLDYETYGGAQLLGIKGVVVIAHGMSSRVAIANALTMAAE
                     AAKHDLPGRLASRLAS"
     gene            184..858
                     /gene="rnc"
                     /locus_tag="VFU96_03800"
     CDS             184..858
                     /gene="rnc"
                     /locus_tag="VFU96_03800"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU5112415.1"
                     /translation="MSLEQQFGYTFSDRELLRLSLTHRSVSSEDGSRRDNERLEFLGD
                     AVLQLAITSYLYDTYPDLPEGNLAKIRAGLVSRPVLADVARSIGLGDHIELTVAEERT
                     GGREKDSITADALEAVIGAVYLDSGLASATDLVLRLWGSRIGERAERPGLKDYKTRLQ
                     EVLARDGKRPVYLTDGSGPDHERTFVASVQVEGIGLGSGSGRSKKEAEQGAAEQALER
                     LIADSK"
     gene            complement(839..1645)
                     /locus_tag="VFU96_03805"
     CDS             complement(839..1645)
                     /locus_tag="VFU96_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112416.1"
                     /translation="MEITPRPFGEIIGEGMTMLGRVWRRILAPAFWAFVLLGAATIAV
                     FALTGAGDVLQLILNDPGALDRMTDAEIGEAGLLLLQAGSVVLLFQLLASGFLSLTVH
                     RIVGSELAGEPLGARSASAQALARLFTLVVAGVIAVVLVTVGLAALILPGLWLAGAFS
                     MVSAVIALEGLGPVQALRRSFALVRGRWWPTVGFLLLVGLFGSVAAQLVQLVALPIVG
                     AGGVGIGAGLGFVVLVVAQGFVVAAIAVMVTLWYLDLRARKEPVVISSLR"
     gene            1687..>2286
                     /locus_tag="VFU96_03810"
     CDS             1687..>2286
                     /locus_tag="VFU96_03810"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEU5112417.1"
                     /translation="MPELPEVEMTRRHLDDAIRGRRLTRVRTTHPRTARHNAGGPAEI
                     DERLVGRTVLGVGRKGKFLTAPLDDGQTLVAHLGMSGRFTIGDPSEAEPPHTHFRARL
                     DDGREVRFVDPRTFGFVAVVTSDEMEGSGLSRVGPDAWDTTPSVEDLAAALARRTAPV
                     KALLLDQVIVSGLGNIYADEALHMAGIHPLRPGGSLSEGE"
BASE COUNT          450 a          747 c          755 g          334 t
ORIGIN      
        1 tgaggtgaga cggcggcttg actatgagac ctacgggggc gctcagctgc tcgggataaa
       61 aggcgttgtc gtcattgccc atggcatgtc gagtcgggtc gccatcgcca atgccttgac
      121 catggcggcc gaagcggcca agcatgacct ccccggccgg ctggcgagtc gtctcgcctc
      181 gtgatgagcc tggaacagca gttcggttac accttctccg accgtgagct gctcaggttg
      241 tcgctcacac atcgctcggt cagcagtgaa gatggctcga ggcgagacaa cgagcgactc
      301 gagttcctcg gggatgctgt gctccagttg gccatcacga gctatctgta cgacacctat
      361 cccgacctcc ccgagggcaa cctggccaag atcagggcgg gcctggtgag ccggccggtg
      421 ttggccgatg tcgcccgcag catcggtctc ggcgaccaca tcgagttgac cgtcgccgaa
      481 gagcggacag ggggacggga aaaggattcg atcaccgccg atgctctcga agccgtgatc
      541 ggggcggtct acctcgactc cggcctcgcg agcgcgaccg acctggtgct acgcctctgg
      601 ggcagccgca tcggcgagag agcggagcga cccgggctca aggactacaa gacccggctt
      661 caggaagtgc tggccagaga tggcaagcgt cccgtgtacc tcaccgacgg gtccggcccc
      721 gatcatgagc ggaccttcgt cgccagcgtc caggtggaag ggatcggcct gggctcagga
      781 tcgggtcggt ccaagaagga ggccgagcaa ggcgctgccg agcaggccct cgaaaggctc
      841 atcgcagact cgaaatgacc accggctcct tgcgtgccct gagatcgagg taccagagag
      901 tgaccatcac cgcgatcgcg gccaccacga atccctgagc caccacgagc acgacgaatc
      961 ccaatccagc tccgataccc acgccgccgg cgccgactat cggcagggcg accaactgca
     1021 ccaattgtgc ggcgaccgag ccgaataggc cgacgagcaa gagaaacccg accgtcggcc
     1081 accacctgcc gcgtaccagt gcgaagctcc tgcgcaacgc ctgcaccggc ccgagtccct
     1141 ccagggcaat gaccgcggac accatgctga aggccccagc gagccacaac ccggggagga
     1201 tcagagcggc caggcccacc gtgaccagga ccacagcaat caccccggcg acgacgagag
     1261 tgaacagcct cgccagtgcc tgagcggacg cggatcgcgc gccgagaggc tcgccggcga
     1321 gctctgaccc aacgatgcgg tgcaccgtca ggctcagaaa gcccgaagca aggagttgga
     1381 agaggaggac tactgagccc gcctgaagca acaggagacc cgcctcaccg atctcggcgt
     1441 cggtcatccg atcgagggca ccgggatcat tcaatatgag ctggaggaca tcaccggcgc
     1501 cggtgagggc gaaaacagcg atggtggccg ctccgaggag aacgaaggcc cagaacgcgg
     1561 gagccaggat ccggcgccag acccgaccca gcatcgtcat gccctcacca atgatttcgc
     1621 caaagggcct gggggttatc tccatgggcc ggggtacggt aaccagccct cgatcaggag
     1681 tgatccttgc ccgagttgcc ggaagtggag atgacgcgtc gccatctgga cgatgcgatc
     1741 agagggcgtc ggctgacccg cgtccgcacc acgcaccctc ggacggcccg tcacaacgca
     1801 ggtgggccgg ccgagatcga tgagcggctc gtggggcgga cggtgctcgg ggtgggacgg
     1861 aaggggaagt tcctcacggc ccccctcgat gacggtcaga ctctcgtggc ccacctcggg
     1921 atgtcggggc gattcacgat cggcgacccg tcggaggcgg agcccccaca cacccatttt
     1981 cgggcgcgat tggacgacgg acgtgaggtc cgattcgtcg atccgcggac cttcgggttc
     2041 gtcgccgtcg ttaccagtga tgagatggag ggctcgggtc tgtcccgtgt cgggcccgac
     2101 gcatgggaca ccacgccgtc ggtcgaagac ctggcagcgg ctctcgctcg caggaccgcc
     2161 ccggtcaagg cgcttctact cgaccaggtc atcgtctccg gtctgggcaa catctacgcc
     2221 gacgaggctc tgcacatggc aggtattcat cccctgcggc ccggcgggag cctgtcggaa
     2281 ggagag
//
LOCUS       DASTXO010000180         2187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1116251, whole genome shotgun sequence.
ACCESSION   DASTXO010000180 DASTXO010000000
VERSION     DASTXO010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1116251"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VFU96_03815"
     CDS             complement(<1..405)
                     /locus_tag="VFU96_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEU5112418.1"
                     /translation="MPKSLVIVESPTKARTISGFLGPEVAVESSMGHIRDLPRSAAEI
                     PASAKGKDWARLGVDVDNDFEPLYVVPKERKERVKKLKEMLAGVDQLYLATDEDREGE
                     SIAWHLAEALKPKVPVHRMVFHEITQHAIEDAF"
     gene            485..1099
                     /gene="tmk"
                     /locus_tag="VFU96_03820"
     CDS             485..1099
                     /gene="tmk"
                     /locus_tag="VFU96_03820"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU5112419.1"
                     /translation="MTGETPRNHRYIALEGGDGSGKTTLSAALAARLRSSGDEVVEVR
                     EPGGTPLGETVRELVLDSEQVDHWAEVFLFAAQRAQLARDVVAPALQRGAWVISDRTY
                     YSSIAYQGRARGLGEEQVREINEIGLEGVEPSRVFVIDIDAGAALRRQGRPDRIGSES
                     VDFQKEVSAAYRDLALAEPSRVLLLDGSGSVEELVDRIMMELGG"
     gene            1096..2133
                     /locus_tag="VFU96_03825"
     CDS             1096..2133
                     /locus_tag="VFU96_03825"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HEU5112420.1"
                     /translation="MSDPFDGVIGHERVVDLLRRELGGPAQAYLFTGPDSVGKGTLAE
                     RFAAALLCAEGGEHEVECRSCRLVRGGVHPDVTVVEPEGATSLGVDQARAVVTRASLR
                     PVESDRSIFLFPEAGTMTEQAANALLKTLEEPTASVVFLLVAESEDDFPATVASRCRT
                     IHVGRVPLATMVQALEARGLDQTLAEGVAVVSGGRPGLALALMDRPEVSRFRDLWLAI
                     PGQVTPHPGDGQRLATEVLSEIAPLVDQSISEEMTKDREQRARRRAEQSLLVNGLEIL
                     ASWYTDSASLQMGGPVRNSDLPLGSFTDVSPRKAVASAEKILDAVVDIQANLRRELVL
                     ANLFAGLGSET"
BASE COUNT          359 a          641 c          762 g          425 t
ORIGIN      
        1 aaaggcgtcc tcgatcgcat gttgggtgat ctcgtggaac accatgcgat gaaccggcac
       61 cttgggtttg agcgcttcgg cgaggtgcca ggcgatggac tcgccttctc ggtcctcgtc
      121 tgttgccaga tagagctggt caacgccggc gagcatctcc ttgagcttct tgactcgttc
      181 cttccgctcc ttggggacca cataaagcgg ctcgaaatcg ttgtccacgt cgacaccgag
      241 gcgggcccaa tccttgcctt tggcgctggc cgggatctcg gcggccgacc gcggaaggtc
      301 ccggatatgg cccatcgacg actcgacagc tacctccggt ccgaggaatc cagagatagt
      361 gcgggccttc gtcggggact cgacgattac gaggctctta ggcatgggtg gggagagttg
      421 acttcatctg accgcctgat gtcaatacga ctcgtgtctc tggagaccat taccgtccat
      481 cacagtgaca ggggaaacgc ctcgtaacca ccgatatatt gcgctggagg ggggcgatgg
      541 cagcggcaag acgactttgt cggccgcctt ggccgccagg cttcgctcct cgggcgatga
      601 ggttgtcgag gtgcgtgagc ccggtgggac cccgctcggt gagacagttc gtgagctggt
      661 gctcgactca gaacaggtcg atcattgggc cgaggtgttc ctcttcgcag cgcaacgagc
      721 tcagctcgcg cgggacgtgg ttgctccggc tctgcagcga ggtgcctggg tgatcagcga
      781 tcgcacttac tactcatcca ttgcctatca gggacgggcg cgtgggctcg gcgaggagca
      841 ggtgcgtgag atcaacgaga tcgggctcga gggtgtggag ccaagccgtg ttttcgtgat
      901 cgatatcgac gcgggcgcgg cattgcggcg tcaaggacga ccggatcgga tcgggagcga
      961 gagtgtcgac ttccagaagg aagtgagcgc cgcataccgc gacctcgcat tggccgagcc
     1021 cagccgagtc cttctgctgg acggatccgg gtcggtcgag gagctggtgg atcggataat
     1081 gatggagttg ggcggatgag cgatcccttc gatggagtga tcggccacga gcgggtggtc
     1141 gatctcttgc gtcgtgagtt gggagggccg gcccaggcgt acctgttcac cggccccgac
     1201 tcggtgggca aggggacgct cgcggagcgg ttcgccgcgg ccctgctgtg tgcggaaggt
     1261 ggcgagcacg aggtggagtg ccggtcatgc cggttggtgc gcgggggcgt ccatcccgat
     1321 gtgactgtgg tcgagccgga gggcgcgacc agtcttggtg tcgaccaggc ccgggccgtg
     1381 gtcaccaggg cgtcgctgcg cccggttgag tcagaccgat cgatattcct cttccccgag
     1441 gcggggacga tgaccgagca ggcagccaac gcgcttctca agacactgga ggagccgact
     1501 gcatcggtgg tgttcctgct ggtggcggag tcggaggacg attttcccgc cacggtggcc
     1561 tcccgatgcc gcaccatcca tgtcggcagg gttcccctgg cgacgatggt gcaggccctc
     1621 gaggcccgag ggctggatca gaccctggcc gaaggtgtgg cggtcgtctc gggcgggcgg
     1681 cccggtctgg ctctggcgtt gatggatcgg cccgaagtct cccggttccg cgatctgtgg
     1741 ttggcgatac ccggacaggt gacgcctcat ccgggagacg gacagcggct ggccaccgaa
     1801 gtgctgtctg agatcgctcc tttggtggac cagtcgatct ccgaggaaat gaccaaggat
     1861 cgcgagcaac gggctcgacg acgcgccgag cagtctttgc tggtcaacgg gctcgagatc
     1921 ctcgcctcgt ggtacaccga ctcggcctcc ttgcagatgg gcgggcccgt ccgcaacagc
     1981 gacctgccct tggggtcatt caccgacgtg agccctcgca aggcggtggc gtcggctgag
     2041 aagatcctcg atgccgtcgt cgacatccag gccaatctca gacgcgagct ggtcctggcc
     2101 aatctctttg ccggtctcgg ctccgagacc tgacttttct ccgattcctc agctgacaca
     2161 ccgcggaatc gtttgacggc gaagagg
//
LOCUS       DASTXO010000181         3109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1337477, whole genome shotgun sequence.
ACCESSION   DASTXO010000181 DASTXO010000000
VERSION     DASTXO010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337477"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VFU96_03830"
     CDS             <1..250
                     /locus_tag="VFU96_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU5112421.1"
                     /translation="QMMRDLVYILAPERIILGGGASHLPGLLDQVNQKLIDELSGYPG
                     LPEHESRFVVPPGLGEMSGLAGGIVLAEGAAGRAMSSG"
     gene            complement(234..1253)
                     /locus_tag="VFU96_03835"
     CDS             complement(234..1253)
                     /locus_tag="VFU96_03835"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU5112422.1"
                     /translation="MGLLDEIREQPEVIARTTSVNAGPAQQVAGLVSGCTHAVIAARG
                     TSDNAGRYAQYVWGTRNGLSVGLTTPSLFSVYDRPPRLDGALVVGISQSGESPDIVSV
                     LDEGRKQGRPTVAITNEPDSPLADVADVVIDLHAGEERAVAATKTYTAQLAVVALISE
                     AMAGKSATLDLLSPLVEQALTQEALAAEAAAAHAGMEVCVVLGRGFNQATAFEWALKL
                     QELTQVVAQPFSTADFLHGPIAVLEPGYPVLAIAARGPAIDDVVDVLDRCRQAGASLV
                     VIGNDRRLDGMAPKVLQLDADLDEWLSPIPAVVLGQLFAYHLTVAKGLDPEQPRGLHK
                     VTRTT"
     gene            complement(1253..2158)
                     /gene="nagA"
                     /locus_tag="VFU96_03840"
     CDS             complement(1253..2158)
                     /gene="nagA"
                     /locus_tag="VFU96_03840"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEU5112423.1"
                     /translation="MTGHRSTTGFLDIQINGGWGHDFTADPSSIWEVGRRLPATGVTA
                     FLPTIVSAPYEQVDAAIEVMGSGPPPGYDGADPVGLHVEGPWISPEWKGAHNPDHLRL
                     PDMGIARHWAESGVVRMVTLAPELDGAFEAAGILSKSGVVVSAGHSGADFETAAGALS
                     GAWTAVTHLFNQMSPFHHRSPGLVGAAFLSDRPCGVIVDGIHADPSAVRLAWDHLGPD
                     RFVLITDAMQATGLGEGIYILGDREVFVGPEGPRIGRAILAGSTLTMDQAMANLLDWT
                     AATEDEAHASASIVPSRLLGLETGG"
     gene            complement(2155..>3109)
                     /locus_tag="VFU96_03845"
     CDS             complement(2155..>3109)
                     /locus_tag="VFU96_03845"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEU5112424.1"
                     /translation="AGAELLMVAHQVVPALTGSREVSICASETGINGFVRSELGFDRV
                     VISDALDMGALDQGPAQVVEIIAMMRAGTDLLLCMPDPDLQERARLALDRGHARGLIP
                     DDTLKAAMARVERARSSVRPTQIRPERVGSAGHRQLAMRLAEASITLVRNDDSLIPLR
                     PEAGQRLLCLEPEPVNVTPADTTALYPPQLASALSARHPGVTATVYPHNPTPSDIAAV
                     VAQAKDHDVIVLGTVTATSGQIDLAQTLLGLGKPMVTLALRTPFDLGTYPGSSTHACT
                     YGAHRPSLDALTAALFGHIPFRGHLPVAIPGLHERGHGIET"
BASE COUNT          524 a          981 c         1076 g          528 t
ORIGIN      
        1 ccagatgatg cgggatctgg tctacatcct ggcgccggag cgcatcatcc ttggcggtgg
       61 agcgtcgcac cttccaggct tgctcgacca ggtcaaccag aagttgatcg acgagctctc
      121 cggctacccg ggccttcccg agcacgaatc gagattcgtg gtgccaccgg gtcttgggga
      181 gatgtccggg ttggccgggg gcatcgtcct ggccgaggga gctgccggtc gagctatgtc
      241 gtccgggtga ccttgtgcag accgcgcggt tgctcggggt cgaggccttt ggcgacggtc
      301 aggtggtagg cgaacaactg tcccaggacc accgctggga tgggggacaa ccactcatcg
      361 aggtcggcat cgagctggag gactttgggt gccatcccgt ccagccttcg gtcattgccg
      421 atgaccacca gcgatgcccc ggcttggcgg cacctgtcca ggacgtccac gacatcgtct
      481 attgccggtc cgcgagccgc gatcgccagc accggatacc cgggctccag gacggcgatc
      541 gggccgtgga ggaaatcggc ggtggagaag ggctgggcca cgacctgggt caactcctgg
      601 agtttgagcg cccactcgaa cgcggtggcc tggttgaagc cgcgacccag cacgacacag
      661 acctccatcc cagcatgcgc cgcggctgct tcggccgcga gcgcctcttg ggtgagtgcc
      721 tgctcgacca gaggagaaag caggtcgagg gtcgccgatt tgccggccat ggcctctgag
      781 atcaacgcca ccaccgccag ttgggcggtg taggtcttgg tggcggcaac ggccctctcc
      841 tcgccggcat gcagatcgat caccacgtcg gctacatcgg ccaagggcga gtcgggctca
      901 ttggtgatgg cgaccgtggg acggccctgt ttccttcctt cgtcgagcac cgagacgatg
      961 tccggcgatt cgccgctctg cgatattccg accaccaagg ccccatcgag ccggggaggg
     1021 cggtcataga cggagaacag ggacggtgtg gtgagcccca ccgagaggcc gttgcgggtg
     1081 ccccacacgt attgggcata gcggccggcg ttgtccgagg tgccacgggc cgcaatcacg
     1141 gcgtgggtgc atcccgatac gagccctgct acctgttgag ccggccctgc gttgacactc
     1201 gttgtccggg caatgacctc aggctgttcg cggatctcgt cgagaagtcc catcagccac
     1261 cggtctccaa cccgagcagc cgtgatggga caatcgaagc gctggcgtgg gcttcgtcct
     1321 ccgtggctgc ggtccagtcg aggagattgg ccattgcctg gtccatggtc agggtgcttc
     1381 cggcgaggat ggcccggccg atccgcgggc cttcgggccc aacgaaaacc tcccggtcac
     1441 cgaggatgta gatgccctca ccgagacctg tcgcctgcat cgcatctgtg atcaggacga
     1501 atcggtctgg cccgagatgg tcccaggcca gccggacggc cgacggatcg gcatgaatcc
     1561 cgtccacgat cacgccacac gggcgatcgg acagaaatgc ggcaccgacg agaccggggg
     1621 agcggtggtg aaaggggctc atctggttga agaggtgggt cacggccgtc cacgccccgg
     1681 acagagcgcc cgccgccgtc tcgaagtcgg ccccgctgtg ccccgccgag accaccacgc
     1741 cggacttcga gaggatcccg gcagcttcga aggcgccgtc cagctcggga gccaaggtca
     1801 ccatgcgaac cacacccgat tcggcccaat gccgggcgat ccccatgtcg gggagtcgga
     1861 ggtgatcggg gttgtgagca cccttccatt ccggggagat ccaagggccc tcgacgtgga
     1921 gaccgaccgg gtcggcgccg tcataacccg ggggcggccc ggaccccatg acctcgatcg
     1981 cagcgtcgac ttgctcgtat ggcgcggaga cgattgtggg taggaaggcg gtgacaccgg
     2041 tggcggggag acgccgtccc acttcccaaa tgctcgacgg gtcggccgtg aagtcgtgcc
     2101 cccagccacc gttgatctgg atgtcgagaa acccggttgt gcttcggtgc ccggtcatgt
     2161 ctcgatcccg tgtccccgct cgtggaggcc ggggatggcc acgggcaagt gcccgcggaa
     2221 cgggatatgg ccgaacaggg cagcggtcag ggcgtcgagc gacggccggt gggcgccgta
     2281 ggtgcacgca tgtgttgaag acccgggata tgtgcccagg tcgaacggtg ttcgcagcgc
     2341 gagcgtgacc atcggctttc ccaacccgag caaggtctga gcgaggtcga tctgccctga
     2401 ggtggcagtg accgtcccga gcacgatcac gtcatggtcc ttcgcctgtg ccacgacggc
     2461 tgcgatgtcg gacggtgtgg gattgtgggg gtagacggtt gcggtcacgc cggggtgccg
     2521 ggcgctgagg gcagatgcga gttgtggcgg gtaaagggcg gtggtgtctg ccggcgtgac
     2581 gttcactggc tccggttcca ggcagagcaa gcgttgcccg gcttccgggc gaagcgggat
     2641 gagtgaatcg tcgttgcgga ccagggtgat cgacgcctcg gcaagacgca tcgcgagctg
     2701 gcgatgaccc gctgatccca ccctttctgg tcggatctgg gtcgggcgta ccgaactccg
     2761 cgctcgctcc acccgggcca tcgcagcctt cagagtgtcg tccgggatca acccgcgggc
     2821 gtggccccga tccaatgcca atcgggcgcg ctcctggagg tcagggtcgg gcatgcacaa
     2881 gaggaggtcg gtgcccgccc gcatcatggc gatgatctcc accacctgag ctggtccttg
     2941 atcgagtgca cccatgtcga gagcatccga gatcaccacc cgatcgaacc ccagctcgga
     3001 tcggacgaat ccgttgatcc cggtctcgga ggcacatatc gaaacctcgc ggctgccggt
     3061 gagggcaggg acgacttggt gggccaccat caacaattcc gctccagcg
//
LOCUS       DASTXO010000182         2101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1116335, whole genome shotgun sequence.
ACCESSION   DASTXO010000182 DASTXO010000000
VERSION     DASTXO010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1116335"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VFU96_03850"
     CDS             <1..519
                     /locus_tag="VFU96_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5112425.1"
                     /translation="AEIEGRRQALEYARFLIDRVPGYENASLAVLSSQIGVRETRRVY
                     GDYRVTREDVLAARQFDDQIGLCGAPIEDHIPGKTTRWEYLPEGKAVGIPFSSLVARD
                     GVNVLTPGRCFSATHDAQASIRSMAQCMAMGQAAGTAAAMAATKNGLVREVDGDRLRA
                     ALRADRAILEMP"
     gene            519..1421
                     /locus_tag="VFU96_03855"
     CDS             519..1421
                     /locus_tag="VFU96_03855"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112426.1"
                     /translation="MAFARTVLGDIDADELGVTLSHEHLIIDSPMVAREWPHIHLASV
                     SDAIAETIELAASGVRTMVDAMPIGCGGDAARLADVALATGCNLVASTGMHTVKYYSD
                     GDWHLEASAEDLAKTFISAIEGPAPAAGLIKVATSGPAPTPLEHRLFEAAAMTIEATG
                     VPLLTHCEAGQGGAAQIELIAASGVPLTRVAMSHTDKVPDTAYHRDLLESGVLLCLDQ
                     GLRQPEETARILVDLIEGGFTAQLLVGTDGARRSLWSTLDGAPGLAWINTGFRAMLAA
                     AGIDASSLDMIFRANPGRWLGLSV"
     gene            complement(1418..>2101)
                     /locus_tag="VFU96_03860"
     CDS             complement(1418..>2101)
                     /locus_tag="VFU96_03860"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEU5112427.1"
                     /translation="VGLSPNFEVFAVEEWPGIVAQQLNRRMSEHPGSKLCVATGNTTR
                     PVYRETTITGDPEIFLLDEFGGLPSNDPARCSAMLRSALPGYGFHVPDVDSDDPAAAA
                     TEYRALIDEGGLDLALVGLGGNGHLGMNEPGSTRDSVTRVVELARSTSSGALAYGATI
                     QPSWGITIGIRELLEARELWLLVTGRHKRDILTSVVTGAIGPDLPATFVREHPNAFVL
                     ADAAAAAAF"
BASE COUNT          354 a          689 c          711 g          347 t
ORIGIN      
        1 gccgagatcg aaggccgccg ccaggcactc gagtacgcgc ggttcctcat cgaccgcgtc
       61 ccgggttatg agaacgcctc gctggccgtg ttgagcagcc agatcggcgt gcgggagacg
      121 aggcgggtgt atggcgacta ccgggtcacc cgggaagatg tgctggctgc gcggcaattc
      181 gacgaccaga tcgggctctg tggggcccct atcgaggacc acatcccggg gaagacgacc
      241 cgctgggagt atctgcccga aggcaaggcg gtgggcatcc cgttctccag cctggtggcc
      301 cgcgacggcg tgaacgtgct gacgccaggc aggtgcttct cggccactca cgacgcccag
      361 gcctcgatca ggtcgatggc gcagtgcatg gccatgggcc aggcggccgg aacggcggct
      421 gccatggccg ccacgaagaa cggcctggtc agagaagtgg acggggacag gctccgcgct
      481 gcgctccgtg ccgaccgagc cattctcgag atgccctgat ggcctttgct cggacggtgc
      541 tcggcgacat cgacgccgat gagttgggcg tgactctctc ccacgagcac ctgatcatcg
      601 acagcccgat ggtcgcccgg gaatggccgc acatccatct cgcctcggtc tccgatgcga
      661 tcgccgagac gatcgagctc gccgcgtccg gggtgcggac catggtcgac gccatgccga
      721 ttggatgtgg tggcgatgcg gcccgtctcg ccgacgtggc cctggccacc ggctgcaacc
      781 tggtcgcctc caccgggatg cacaccgtca agtactactc ggacggcgat tggcacctcg
      841 aagcctcagc ggaggacctg gcgaagacgt tcatttcggc gatcgaaggc ccggcccctg
      901 cggcggggct gatcaaggtg gcgaccagcg ggcccgctcc cactcccctg gaacaccggc
      961 tgttcgaagc ggcggcgatg acgatcgagg caaccggtgt gccgttgctg actcattgcg
     1021 aggcaggcca aggcggtgcg gcccagatcg agctcatcgc cgcgtccggg gtccccctca
     1081 ccagggtggc gatgtcgcac acagacaagg tgcccgacac ggcatatcac cgagacctgc
     1141 tcgagagtgg agtccttctc tgtctcgatc agggtcttcg ccaacctgag gagacggcgc
     1201 gaatcctggt ggacctgatc gaaggaggct tcacagctca gctcctcgtc gggaccgacg
     1261 gcgcccgccg ctcgctgtgg tcgaccctgg acggcgcgcc cggtttggca tggatcaaca
     1321 ctgggttccg agccatgctc gccgcagcag ggatcgacgc gagctcactc gacatgatct
     1381 ttcgtgccaa tcccggcaga tggctgggac tatccgtcta gaaggcggcg gcggctgcgg
     1441 cgtcggccaa cacgaaagcg tttggatgct ctcgcacaaa ggtcgccggc aggtcagggc
     1501 cgatggcgcc ggtcacgaca ctggtcagga tgtctctctt gtgccgcccg gtcaccagca
     1561 gccaaagctc gcgcgcctcg agcagctcgc ggatcccgat ggtgattccc caactgggtt
     1621 ggatggtggc cccgtaggcg agggcgccgg aggaggtgga tcgcgccagc tcgaccaccc
     1681 gggtcaccga gtcgcgagta gaccccggtt cgttcatccc gagatgaccg ttccccccca
     1741 gcccaacgag ggccaggtcg aggcccccct cgtcgatgag tgcccggtat tccgtcgcgg
     1801 cggcggccgg gtcgtcggag tcgacgtccg gcacgtgaaa cccgtagccg ggtagagcac
     1861 tgcgcagcat tgcagagcaa cgagcggggt cgttgcttgg tagcccaccg aactcgtcga
     1921 gcaggaagat ctcaggatcc ccggtgatcg tggtctcccg gtacacaggt ctggtcgtgt
     1981 ttccggtggc gacgcacagc ttcgatccgg gatgctccga catcctccgg ttgagctgtt
     2041 gcgccacgat gccaggccat tcctcaacgg cgaagacttc gaagttgggg ctcaacccga
     2101 c
//
LOCUS       DASTXO010000183         2216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_563765, whole genome shotgun sequence.
ACCESSION   DASTXO010000183 DASTXO010000000
VERSION     DASTXO010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563765"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VFU96_03865"
     CDS             <1..655
                     /locus_tag="VFU96_03865"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5112428.1"
                     /translation="LHDEEGNVAIPGLVSEEVGGIDVPEALARELAGCVDGLEQIGTG
                     TIPSRIWTRPAVSILAVDAPPVSEAINQLVPVARAKVSMRTAPGQDTDEALEALKTYL
                     EANAPWGTKLEFFHEERGDAVSLDMDTFAVEAWSAAFIEAFGNEPVRMGAGGSIPFIS
                     TFSEMYPGVPILVIGTSDPTSSYHAPNESQDLADLERAVLSEAIAFRLIAAGISVTP"
     gene            complement(639..1103)
                     /locus_tag="VFU96_03870"
     CDS             complement(639..1103)
                     /locus_tag="VFU96_03870"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018776474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEU5112429.1"
                     /translation="MFDIVFFQPEIPPNTGNAMRLAAVTGCHLHLIAPLGFEMDDARL
                     RRAGMDYREMASVTVHDNMESWHAARRPDRVFAYTVDADRLFTEVDYRPGDCLMFGPE
                     SVGLPDEVKELQWVTERLRLPMRPGVRSLNLSNSAAIVVYEAWRQNGFRGSR"
     gene            complement(1105..1773)
                     /gene="nucS"
                     /locus_tag="VFU96_03875"
     CDS             complement(1105..1773)
                     /gene="nucS"
                     /locus_tag="VFU96_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014913078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HEU5112430.1"
                     /translation="MRLVIARCTVDYSGRVTAHLPEAVRLIMIKADGTLAIHSDGNAS
                     KPLNWMVAPNVVDISEDRWEVTNGKGEKLVIAISEVLEDVTHSLGDDPGLQKDGVEAH
                     LQELLAVRPWVLEEGLGLIRREYPTDIGPVDLLCVDQEGRVVAVEVKRRGEIDGVEQL
                     SRYLERLGQDPRLGPVRGMFVAQLITRQAHVLAEARGIAAIEVDYDGLRDIEDTELRL
                     FDLR"
     gene            complement(1770..>2216)
                     /locus_tag="VFU96_03880"
     CDS             complement(1770..>2216)
                     /locus_tag="VFU96_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112431.1"
                     /translation="AGVGLVGVIARITWGSASERWLGHGRTLRLLAAQSTVAALLLAL
                     ASADVLSSWVLIPAALLIGAGAVAWNAVGMLAVMDFSLPELVGRGTGLAMLGFLTGIG
                     LGAPLMGLSVDRLGDYTPGWVVLATLFLVSAVVAGRIHRTGTLAHV"
BASE COUNT          411 a          742 c          700 g          363 t
ORIGIN      
        1 gctccacgac gaagaaggga atgtggcgat ccccggcttg gtcagcgagg aggttggagg
       61 gatcgacgtt ccggaagcgc tggctcgtga gctcgcgggc tgcgtggacg gcctggagca
      121 gatcggcacc gggaccatac cctcccggat ctggacccgg cccgcggtct cgatcctcgc
      181 cgtcgatgcg ccacccgttt ccgaggccat caaccaactg gttccggtgg cacgggcaaa
      241 ggtgagcatg cgaaccgcgc cgggtcagga caccgacgaa gcgctcgaag cgctgaagac
      301 gtatctcgag gcgaacgcgc cttggggcac caagctggag ttcttccatg aggaacgggg
      361 tgacgccgtc agcctcgaca tggacacgtt cgccgtcgag gcctggtcgg cggctttcat
      421 tgaggccttc ggtaacgagc cggtccgcat gggggcgggg ggttcgatcc cgttcatctc
      481 caccttctcc gagatgtatc ccggcgtccc gatcctcgtc atcgggacca gtgacccaac
      541 cagctcctat cacgccccca acgagagcca ggacctggcc gacctggagc gagcagttct
      601 ctcggaggcg atcgcgttcc gtctcatcgc ggccggaatc agcgtgaccc cctgaatccg
      661 ttctgcctcc acgcctcgta gaccacgatt gccgccgagt tggagaggtt gagcgagcgc
      721 actccgggtc tcatcggcaa ccggaggcgt tccgtcaccc actgcagctc cttgacctca
      781 tctggtagtc ccaccgactc cggaccgaac atcaggcagt cccccggtcg gtaatccact
      841 tcggtgaata gtcgatcggc atccacggtg taggcgaaga ctcgatccgg ccggcgcgct
      901 gcgtgccagc tctccatgtt gtcatgaacg gtgacactgg ccatctcccg gtaatccatg
      961 ccggcccgac gcagcctggc gtcgtccatc tcgaagccga gcggcgcgat gaggtggagg
     1021 tggcatccgg tcacagcggc gagacgcatt gcgttgccag tgttgggagg tatctcgggc
     1081 tggaagaaaa cgatgtcgaa cacttcagcg caggtcgaag aggcgaagct cggtgtcctc
     1141 gatatcgcgc aggccgtcgt aatcgacttc gatcgccgcg atccctctcg cttcggccaa
     1201 cacgtgcgcc tgtcgggtga tcagctgggc gacgaacatc ccgcgcacgg gccccaggcg
     1261 cggatcctgg cccaatcgtt ccaggtagcg gcttagttgt tccacgccat cgatctcgcc
     1321 ccttcgcttc acctcgacag ccaccacgcg cccttcctga tcgacgcaga ggaggtcgac
     1381 cggcccgatg tcggtcgggt attcgcggcg gatcagaccg agaccctcct ccaggaccca
     1441 cgggcggacc gccagcagct cctggagatg ggcctcgacg ccgtccttct gcaatcccgg
     1501 gtcatcaccc agggagtgag tcacatcctc gagcacttcg gagatggcga tgacaagctt
     1561 ctcgcccttc ccgttggtga cctcccaccg gtcctcagaa atgtcgacga cattcggtgc
     1621 caccatccag ttgagcggct tggaggcgtt gccgtcggag tggatggcca gggtgccgtc
     1681 ggccttgatc atgatgagac gcactgcctc cggcaggtgg gcggtgacac ggccggagta
     1741 gtcgacggtg cagcgggcga tgaccagtct caaacgtggg cgagggtacc ggtgcgatgg
     1801 atcctgccgg cgaccacggc gctcacgaga aagagggtcg ccagtaccac ccagcctggg
     1861 gtgtaatccc cgagtcggtc gacggacaga cccatcaacg gggcgccgag accgatcccg
     1921 gtcaggaaac cgagcatcgc cagacctgtc ccacgaccaa ccaactcggg caacgagaag
     1981 tccatcaccg ccagcatccc caccgcgttc caggcaacag cacccgcccc gatgagcagc
     2041 gccgccggaa tcaataccca agagctcagc acgtccgcgg aggccagagc gaggaggaga
     2101 gcagcgacag ttgactgcgc cgccaggagg cgcagcgtcc gcccgtgccc gagccatcgc
     2161 tcggaagccg atccccatgt gatccgggcg atgactccga cgaggccgac accggc
//
LOCUS       DASTXO010000184         4178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1484669, whole genome shotgun sequence.
ACCESSION   DASTXO010000184 DASTXO010000000
VERSION     DASTXO010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484669"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VFU96_03885"
     CDS             <1..316
                     /locus_tag="VFU96_03885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5112432.1"
                     /translation="IDVIWPELHRHLPMFDSLKVETSWAGLYDVNTFDGNAILGEWPG
                     IRGLFVATGFSGHGFQQSPAVGRYLAELILDRDHSLDLSRLGARRVLDNEPLYEHAGR
                     LI"
     gene            581..988
                     /locus_tag="VFU96_03890"
     CDS             581..988
                     /locus_tag="VFU96_03890"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5112433.1"
                     /translation="MTRLLAVAHQTAEADEFLAAVRSAAENDHAEVVLLVPATPVKHL
                     ASWTQGESRAIAREKADAARRRLEESGVNVVEARVGDPDPYQAIRDSLNVEDFDRVIV
                     STFPAGISRWLGADLINRLARSISVPVTHVVAH"
     gene            1018..1941
                     /locus_tag="VFU96_03895"
     CDS             1018..1941
                     /locus_tag="VFU96_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020629043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEU5112434.1"
                     /translation="MRALVNEGKGGITVEVVDLRQPEPGPGEALVETRAVAVNRGELR
                     LLRARPGGWHPGQDVAGVIVGQAADGSGPPVGTRVVAWPEQAGWAELVAVPTSHLARL
                     ADSVTFAEAATLPIAGMTALRALRLGGDLAGKRVLVTGAAGGVGRFAVELASGAGATV
                     TGVAADESRAAGLGELGANEVVFGLEDIEGPFEFILDAAGGPSLEAAVRLVAPRGDVV
                     VYGNSSDTPAQISFSDFRGHAGARITAFFVYESGEPPTFGEDLRGLADMVADGRLHPQ
                     IGVQVPWSEANTAFEALANRHVNGKAVLLVE"
     gene            1983..2471
                     /locus_tag="VFU96_03900"
     CDS             1983..2471
                     /locus_tag="VFU96_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEU5112435.1"
                     /translation="MSDLPVDVVRSKRRTRTAQAYIRDGRLRVMVPFGLDHDEEALLV
                     DGLVARVSRKVASTEVDLVQRARDLARDYRLSEPTSIEWSSRQSTRWGSCTPAEGSIR
                     ISVRLASMPGWVLDSVLIHELAHLDEPTHGPRFKALVDRYELAERAKGYLMAMGEGRT
                     SP"
     gene            complement(2509..2802)
                     /locus_tag="VFU96_03905"
     CDS             complement(2509..2802)
                     /locus_tag="VFU96_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010033089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEU5112436.1"
                     /translation="MAITGAKGSLDKTFTAPLQKSPAQGGWTYLVMPGSVEFFGTRGL
                     VRVRGTIDGVDFQTSFMAIGDGTHKLPVKAALRDAIGKSTGEEVTVHLTERLK"
     gene            complement(2793..3182)
                     /locus_tag="VFU96_03910"
     CDS             complement(2793..3182)
                     /locus_tag="VFU96_03910"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HEU5112437.1"
                     /translation="MELNYLAIVVASVAAFILSAVWYGVFGGSLAELHPAYADSGPPS
                     AKDAIVELARNLVVAVVLAGLADQIGIEGWAQAAVLGFALWIGIVVVLLTGSVYHEKV
                     PVKLASIHAGDWLLKLVVVAIIVGVWQ"
     gene            3217..>4178
                     /locus_tag="VFU96_03915"
     CDS             3217..>4178
                     /locus_tag="VFU96_03915"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEU5112438.1"
                     /translation="MRRAGTHRRSAPEGFDGVALIARPGWDLTLLQYPTMIYRMSWLS
                     ASVLATLAVLPPGGALVDNEALVHTSAIEAIAVDSTQPPSGTLLPVGAAWNPIIAAEP
                     AGTTYIVEEGVHYEQTANLKHGDSLIGEPGAVMDGRNVTEWAVQPNNANNLTVRGLEI
                     RNYSPDDQYAPITARTHEGAAAGIGWTVENCDLHHNSYAGLALSDGSIARNNKIHDNG
                     VIGLEVSWVGSSGALVEGNEVYGNNTTGADEYYEAGGSKFGVTENLIVRGNFVHDNNG
                     PGLWTDIDNVNTLYENNVVENNSGAGIFHEISYSATIRNNQVRG"
BASE COUNT          771 a         1279 c         1386 g          742 t
ORIGIN      
        1 catcgacgtc atctggcccg agctgcaccg tcacctgccg atgttcgaca gcctgaaggt
       61 ggagacctcc tgggctggcc tgtacgacgt caacactttc gacgggaacg ccatcctcgg
      121 agaatggccc gggatcagag gactattcgt ggccaccgga ttctcgggcc acgggttcca
      181 gcagagcccg gcggttggcc gttacctggc cgagctgatc ctcgaccggg accacagtct
      241 cgacctgtcc cggctcgggg ctcggcgcgt gctcgacaac gagccgctct acgagcatgc
      301 cggcaggctg atctgaaggc acccgtgaca gatcggtcga gaaggtgtat gttcgaatca
      361 gttcggtcgg acacgtaggc cggaaacggt cggatcacga gaccggcaga gcgccccact
      421 acgtggggac gctcccggga agccctgtgg gcggaaacgt ctgcggggct tctctcattc
      481 acttggtctc agcactctgg cggggtcaac cggcatgaca ggagaccttg acagttggca
      541 caggtagcgc gacggtgacg tggtctgacc gaggtatgtg gtgacacgtc tgctcgcagt
      601 ggcgcatcag actgccgaag ccgatgagtt tctggctgcc gtccgatccg cggcagaaaa
      661 tgatcatgca gaagtggtcc tactggtccc tgccaccccg gtgaagcatc tcgccagctg
      721 gacccaaggc gagtcacgcg caattgcccg ggagaaggcg gatgcagccc ggcgccgact
      781 cgaggagtcc ggggtgaacg tcgtcgaggc tcgggtgggc gatccggatc cctatcaggc
      841 gatccgcgat tctttgaacg tggaggattt cgaccgggtg atcgtctcca ccttccccgc
      901 cgggatctcg cgatggctcg gggccgatct catcaatcgc ttggcccgct cgatatccgt
      961 gcccgtgact cacgttgttg cccattaact ttcggttccc gacacggcgg taggaacttg
     1021 agagcattgg tcaacgaagg caagggcggc atcaccgtag aggtggtcga tctacggcag
     1081 ccggagcccg gccccggcga ggcgctggtc gagacgagag ccgtggcagt caatcgtggc
     1141 gagctccgtc tcctccgagc ccggcccggt gggtggcacc ccggacagga cgtggccggg
     1201 gtaatcgtcg gacaggccgc cgacgggtca ggacccccgg tcgggacgag ggtcgttgcc
     1261 tggccagagc aggcgggctg ggcggagttg gtggcggtcc cgaccagcca tctggcacga
     1321 ctcgccgaca gtgtcacatt cgcggaggcg gcaaccctcc cgatcgcggg catgaccgcg
     1381 ttgcgcgccc tccgactcgg tggtgacctg gcgggaaaac gggtcctggt gaccggggcc
     1441 gctggtggag tggggcggtt tgcagtggag ctggcttccg gggccggggc gacggtgacc
     1501 ggggttgcgg ccgatgagtc gcgcgctgca gggttggggg aattgggggc aaacgaagtc
     1561 gtgttcgggt tggaggacat cgaggggccg ttcgaattca tcctcgatgc tgccggaggc
     1621 ccttctctgg aagcggcggt gcgactcgtg gcgcctcgcg gagacgtcgt cgtctacggg
     1681 aactcgtccg acactccggc acagatctcg ttctccgatt tccgaggtca cgccggcgcc
     1741 cggatcactg ccttcttcgt ctacgagagt ggcgagccac caaccttcgg agaagacctg
     1801 cgggggctgg ccgacatggt cgccgatggc cggctgcacc cgcagatcgg ggttcaggtg
     1861 ccctggtccg aggccaacac tgccttcgaa gctctggcca accggcacgt caacggaaaa
     1921 gcagtgctgc tggtggaatg acgatcgtcg gatctgtcac cccggcgact tacgatccga
     1981 gaatgagcga tctgccagtc gacgtggtcc ggagcaagcg gcgaacgcgg actgctcagg
     2041 cctacatcag agacggtcgg ctgcgggtga tggtgccatt cgggctggat cacgacgagg
     2101 aggcgcttct ggtcgatggc ctggtggccc gggtgagcag aaaggtggcg tccaccgagg
     2161 tcgacttggt gcagcgcgcc agggacctcg cccgtgacta ccggctgtcg gagccgacga
     2221 gcatcgagtg gtcttctcgc cagagcacca gatggggctc atgcactccg gctgagggaa
     2281 gcatccgcat ctcggtccgc ctggcgtcga tgccgggatg ggtcctcgat tcggtgctca
     2341 tccatgagct ggcgcatctc gacgagccca cccacgggcc acgtttcaag gcgttggtcg
     2401 accgctatga gctggcggag cgagcgaagg ggtatttgat ggcgatgggt gagggccgga
     2461 ccagtcccta gacgtcggcg ggccgtccgg acggggtccc tgcccatgtc atttcagccg
     2521 ctcggtgagg tggaccgtta cctcctcgcc tgtgctctta ccgatggcat cacgaagggc
     2581 ggccttcact ggcaatttgt gcgtgccgtc cccgatcgcc atgaaggagg tctggaaatc
     2641 gaccccgtcg atggtgcccc gcactctgac caggccccgg gtcccgaaga actcgaccga
     2701 gcctggcatc accaggtagg tccaaccgcc ctgggccggg cttttctgca gcggcgccgt
     2761 gaacgttttg tcgagtgagc ccttcgcgcc tgttattgcc atacccccac gattatcgcg
     2821 acgaccacca gtttcaagag ccagtcgccg gcgtggatcg aggccagctt caccggcacc
     2881 ttctcgtgat agacggaacc ggtgaggagc acgacgacga tcccgatcca caatgcaaat
     2941 ccgagcacgg cggcctgtgc ccatccctcg attccgatct ggtccgccaa tcccgccagg
     3001 accacggcca ccacaaggtt ccgtgccagc tcgacgattg catccttcgc agagggtggt
     3061 cccgaatcgg cataggcggg atgcaactcg gcaagggagc ctccgaacac cccataccag
     3121 accgcgctga ggatgaacgc cgccaccgac gcaacgacga ttgccaaata gttgagttcc
     3181 atgacatccc tccgtgtcgg gcctgagaag tagaccgtga gacgcgctgg aactcatcgg
     3241 cgatctgctc cagagggctt cgatggagtc gcgctcatcg cccgtccggg ttgggatctc
     3301 acgttgcttc agtacccgac gatgatctac agaatgagct ggctctcggc gtctgtgttg
     3361 gccactttgg cagttctgcc cccgggtggt gcgcttgtcg acaatgaggc gctcgttcac
     3421 accagcgcga tagaggcgat cgccgtggat tccacgcagc cgccatcggg gacactgctc
     3481 ccggtcggcg ccgcttggaa cccgatcatt gcggccgaac ctgccgggac gacctacatc
     3541 gtcgaggagg gcgtgcacta tgagcagacc gcgaatctga aacatggcga ctcactgatc
     3601 ggcgagccag gagcggtcat ggacggcaga aacgtcaccg agtgggcggt ccaaccgaac
     3661 aacgccaaca acctcaccgt cagaggtttg gagattcgca actacagccc cgacgatcag
     3721 tatgcgccca tcacagctcg aacccacgag ggggcggcgg ccggtatcgg ctggacggtc
     3781 gagaactgcg atttgcatca caacagctac gccgggttgg ccctatccga cgggtcgatc
     3841 gcccgtaaca acaagatcca tgacaacggt gtcatcggtc tcgaggtgtc ctgggtgggg
     3901 agttcggggg cgctcgttga aggcaacgag gtctacggga acaacaccac gggagccgac
     3961 gagtattacg aggcgggagg gtccaagttc ggtgtgacgg agaacttgat cgttcgtggg
     4021 aacttcgttc acgacaacaa cggcccgggc ctgtggaccg acatcgacaa cgtcaacact
     4081 ctctatgaga acaacgttgt cgagaacaat tcgggtgcag gcatcttcca tgagatctca
     4141 tactcggcga cgatccgcaa caaccaggtt cggggcaa
//
LOCUS       DASTXO010000185         6258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1558375, whole genome shotgun sequence.
ACCESSION   DASTXO010000185 DASTXO010000000
VERSION     DASTXO010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1558375"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VFU96_03920"
     CDS             <1..813
                     /locus_tag="VFU96_03920"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEU5112439.1"
                     /translation="HAEGPVWSESWGGLRWVDMLDGDVMFLGADGSIERRHVDDIAAI
                     IRPRRSGGAVQGIERGFALEAPDGTITRLDDLWTGGDVRMNEGGCDPDGRFYCGSMAY
                     DKRPGAGSLYRLDADGTVTVVLGGVTISNGLDWSPDDSLAYYVDTPTHEVSVFDYDSV
                     EGLSGRRTFVSIPAEQGGPDGLVVDEEGGVWVALFGGSAVHRYTADGVLDAVVEVGAR
                     QVTACTFGGADLDRLYITTSRENLEPDDDPLAGSLFTIVPGVAGLPVRAYAG"
     gene            complement(810..2567)
                     /locus_tag="VFU96_03925"
     CDS             complement(810..2567)
                     /locus_tag="VFU96_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5112440.1"
                     /translation="MSTLRLVWEVIRYRPGRWFFCFFMWTLVHGLPLLFGILIGLVFD
                     RLAAGSPVDETAWAPAIAFAVIAIGRNLIIWLGDRQWINHWIEQSLQLRRNLLRWLME
                     APGSRVIPLAPGQAVSTFRDDVDDILEYMENWVDMGGLVVFGVGAVAVMASIDAGLTA
                     ILLIPLILTMVLTQALSPQIRKRRRLMRAATDQVTGFIGETFAGVQAIKLAAAEQPVL
                     ERLHRLNETRHKTALSDTVLTEVLRGINRNMSTITVAIVLLVAADSISDGSFSIGDLA
                     LFLTYLPRLTDYMAFVGDIIAQHRRTGVAYERIRALAVDAPDAELLDRDRVPLEGEIP
                     DIELPDIGIDPLQRLEVRGLSHWFPDGGEGLEGVDFEVDRGSFTVVTGKIGSGKSTLV
                     RALLGLVPADGDVSWNGKPVEDRASFFVPPRSAYTAQVPRLFSESLADNIALGQRVAR
                     ERLREAVQLAVLDPDLERLEMGEQTMVGARGVKLSGGQVQRSAAARMLATEAELLVFD
                     DLSSALDIHTETELWRRLLSQRDVTCLVVSHRRAALSRADQILLMDGGRVVDRGALPD
                     LLARSALMRSLWAEEGEAA"
     gene            complement(2564..4306)
                     /locus_tag="VFU96_03930"
     CDS             complement(2564..4306)
                     /locus_tag="VFU96_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017871240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5112441.1"
                     /translation="MTATAPSYRATLSTYLRPHRGRVIVLMLLLLGSIGLQLVIPLIL
                     RDFIDSAMAGAAVAGLTTAGIAYLVAGILNQLLDAGASYLGTDIGWSATNQLREELTS
                     HLFSLDMGFHNDTTPGEMIERVDGDVTAISEFISRFLVRLIAAGVLLTGVVIVCWFES
                     PAMGLTITLYVVAVLAFLIRLRKVAVSAAEEERETSARLYGFIEERLAGVDDIRSLGA
                     GRFTMTRFIPVMRDFYTRTTNAWRKRIMVWVSANTAFWAGDALALIIGVWLTVRGSIT
                     VGTAFLILQYVQLVRRPVEQMTQELQELQKAAGGIIRIDQLRHLGSSIPDGGEDLPAG
                     PLAIEFDDVSFAYEDRGVLDGVSFNLAAGRSLGLLGRTGGGKTTLTRLIARLYDPDQG
                     VVRVGERDIRSVRSESLRRSIGVVTQDVQLFESTVRDNLTFFAERPDEEVLSVLERVG
                     LGPWIGQLGLDTRLGAGGQGLSAGEQQLLAFARVFLQDPGVVILDEPSSRLDPVTEML
                     LATATERLYAGRTVVIIAHRLETVRTADEIMVVDAGRIVEHGSRQALAADPTTRYATL
                     LATGRGDILGDTRP"
     gene            complement(4361..5887)
                     /locus_tag="VFU96_03935"
     CDS             complement(4361..5887)
                     /locus_tag="VFU96_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEU5112442.1"
                     /translation="MLQASLTLDPEFRVGAVQRRTFGSFVEHLGRCVYTGIFEHGHAT
                     ADEDGLRRDVLDLVRELGVTVVRYPGGNFVSGYSWEDGIGPVDQRPARRDQAWHSLET
                     NEFGIAEFARWAGKADVEMMCALNLGTRGAESAVGLLEYCNQPGGTRWSDLRISHGHP
                     EPFGVRLWCLGNELDGPWQIGHKTAEEYGRLAAETARAMRRIDPDLELVVCGSSHSGM
                     PTFGSWEATVLEHTYDEIDYISLHAYYEEHDGDTASFLASALDMEAFIESVVATADQV
                     RARTRSRKRINLSFDEWNVWYISRFQGEGPPTDWPVAPPLLEDTYSIKDAVVVGTLLI
                     TLLRHSDRVTAACQAQLVNAIAPIRTEPGGPAWRQTTFFPFAHASQFARGEVLDPLIS
                     TLSYESSRYGDTPIIDGVATHDRDAGRVVVFAVNRHREEQVRLTVRMRAFPGYRLVQH
                     VLLADDDPDAANTRDQPDRVTPRIGPLPSTDGASFEVVLPPLSWNVLRMETVEVSAAP
                     "
     gene            complement(5942..6133)
                     /locus_tag="VFU96_03940"
     CDS             complement(5942..6133)
                     /locus_tag="VFU96_03940"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEU5112443.1"
                     /translation="MAPDFAAALEAEPDAGTFFDSLAQFYRNAYLRWIDSTKRSPELR
                     AARIAETVELLKAGVKQRS"
BASE COUNT         1105 a         1985 c         2113 g         1055 t
ORIGIN      
        1 cacgcggaag gaccggtttg gtctgagtca tggggcgggc tgcgctgggt cgacatgctc
       61 gatggtgacg tcatgtttct cggcgccgac gggtcgatcg aacgtcggca cgtcgacgac
      121 atcgccgcga tcatccggcc ccgccgatcc ggtggcgcag ttcagggcat cgagcgtggc
      181 tttgccctcg aagctccgga cgggacgatc acccgcctcg atgatctgtg gaccgggggc
      241 gatgtgagaa tgaacgaggg tggatgtgat cccgacggcc gcttctattg cggctcgatg
      301 gcatatgaca aacggcccgg ggcggggtct ctgtaccggc tggatgcgga tggcacggtc
      361 acagtcgtcc tgggcggcgt gacgatctcc aacggtctcg actggagccc tgacgactcg
      421 ctggcttact acgtagacac gccgacgcac gaggtttcgg tcttcgacta cgactccgtc
      481 gaaggcctga gtggccgacg aacatttgtc agcattcccg cagagcaggg tggtcccgac
      541 ggtctcgtgg tcgacgagga gggcggcgtc tgggtggccc tgttcggggg aagcgccgtg
      601 caccggtaca ccgccgacgg cgtgctcgac gcagtggtcg aggtgggcgc gagacaggtc
      661 accgcctgca ctttcggtgg agccgatctc gaccgcctgt acatcaccac ttcgagggag
      721 aacctcgagc ccgatgacga cccgttggcc ggttcgctgt tcacgatcgt gcccggcgtc
      781 gccgggctac cggtgcgggc ctacgcgggc taagccgctt ctccctcttc cgcccaaagc
      841 gagcgcatca acgccgatcg tgccaggaga tcgggcagcg caccgcggtc gacgacccgg
      901 cccccgtcca tgaggaggat ctggtcggcc cgcgaaagtg cggcccgccg atgggagacg
      961 acgagacagg tcacgtcccg ctggctgaga agacgacgcc aaagctcggt ctcggtgtgg
     1021 atgtcgagcg ccgaggagag gtcgtcgaag acgagcaatt cggcttcggt ggccagcatt
     1081 cgcgccgctg ccgatcgttg cacttgcccg cccgacaatt tgacccccct ggccccgacc
     1141 atggtctgtt cacccatctc gagccgttca agatcgggat cgagcacggc gagctgcacc
     1201 gcctcacgca gacgctcacg agcgactctc tgcccgagtg cgatgttgtc agcgagggac
     1261 tcggagaaga gccgggggac ctgggccgtg taggcggaac gcggcggtac gaagaacgac
     1321 gcccggtcct cgactggctt cccgttccac gagacatcgc cgtcggcggg caccaacccg
     1381 agcagggcac ggaccagcgt gctcttgccg gacccgatct tcccggttac cacggtgaac
     1441 gatccccgat cgacctcgaa gtcgacccct tcgagaccct ctccgccgtc cgggaaccaa
     1501 tggctgagcc cacgcacctc cagccgttgc agtgggtcga tgccgatatc ggggagctcg
     1561 atgtcgggga tctcgccttc gagaggcacg cggtcccggt cgagcagctc ggcgtcgggg
     1621 gcatcgacgg ccagggcccg gattcgctcg taggcaaccc cggtccggcg gtgctgggcg
     1681 atgatgtcgc cgacgaatgc catgtagtcg gtcagcctgg gcagataggt gaggaacaat
     1741 gccaggtccc cgatgctgaa gctcccatcg ctgatcgagt cggcggcgac gaggagcacg
     1801 atggccaccg tgatggtcga catgttgcga ttgatgccgc gcaggacctc ggtgagcact
     1861 gtgtcggaaa gcgccgtctt gtgacgggtc tcattgagcc gatgtaggcg ctcgagcacc
     1921 ggctgctcgg ccgcggccag cttgatcgcc tggacaccgg cgaaggtctc tccgatgaac
     1981 ccggtgacct gatccgtcgc cgccctcatc agccgccggc gcttcctgat ctgcgggctg
     2041 agcgcctggg tgagcaccat ggtcagaatc aaggggatga gaaggatggc tgtcagcccg
     2101 gcatcgatcg atgccatcac cgccactgcc ccgacgccga agacgacgag accacccatg
     2161 tccacccagt tctccatgta ctcgaggatg tcgtcgacgt cgtcacggaa ggtgctcacc
     2221 gcttgacctg gggcgagcgg gatgacacgg gagccgggag cctccatgag ccagcgaagc
     2281 agattgcgac gcagctgaag tgattgctcg atccagtggt tgatccattg tctatcgccc
     2341 agccagatga tgaggttgcg gccaatggcg ataaccgcga aggcaatggc gggagcccag
     2401 gccgtctcgt ccaccgggct gccggcggcc aaccggtcga acaccaggcc gatcaggatc
     2461 ccgaacagca gcggcagccc gtggacgagg gtccacatga agaagcagaa gaaccatctg
     2521 cccggccggt atcgaatcac ctcccagacg agacgcaaag tgctcatggc ctcgtgtccc
     2581 ccaggatgtc gcctcgcccg gtggcgagca gggtggcgta cctggtggtc gggtcggcgg
     2641 ccagggcctg tcgggacccg tgctcgacga tccggccggc atcaacgacc atgatctcat
     2701 ccgccgtgcg caccgtctcc agacgatgag cgatgatcac gacggtgcgg cctgcgtaga
     2761 gcctctcggt ggcggtggcg agcagcatct cggtcaccgg atcgaggcgt gacgagggct
     2821 cgtcgaggat cacgactccc ggatcctgca gaaagacacg agcgaaggcg aggagttgct
     2881 gctctccggc ggagagcccc tggccacctg cgccgagcct ggtgtccagg ccgagctgcc
     2941 cgatccaggg gccgagacca acacgctcca gaacggacag gacctcttcg tcgggccgct
     3001 ccgcgaagaa ggtgaggttg tccctgactg tcgactcgaa gagctgtacg tcctgggtga
     3061 cgacgccaat cgaccgacgg agagactccg aacggacgct tcggatgtct cgttctccga
     3121 cccgcacgac gccctggtcg gggtcgtaga gccgggcgat gagacgggtg agggtggtct
     3181 tgcccccgcc ggtccgtccg agcaggccga gtgatctgcc ggcggcgagg ttgaagctga
     3241 ctccatcgag cacgccgcgg tcctcgtagg caaacgaaac gtcgtcgaac tcgatcgcca
     3301 ggggcccggc gggaaggtcc tcgccaccat ccgggatcga ggacccgaga tggcgtagct
     3361 ggtcgatgcg aatgatcccg cctgccgctt tctgcagctc ctggagctcc tgggtcatct
     3421 gctcgacggg tcgccgcacc aattggacgt actggaggat caggaaggca gtccccaccg
     3481 ttatcgaccc gcgcacggtg agccacacac cgatgatcag ggccagggcg tccccggccc
     3541 agaaagcggt gttggccgac acccagacca tgatccgctt ccgccaagcg ttggtggttc
     3601 gggtatagaa gtcccgcatc acggggatga atcgggtcat ggtgaaccgg ccggccccga
     3661 gtgagcggat gtcgtcgact ccggccagac gctcctcgat gaacccgtag agacgggccg
     3721 acgtctctcg ttcctcctcg gcggcggaga ctgccacctt gcggagtcgg atgaggaagg
     3781 cgaggaccgc cacgacgtag agcgtgatgg tcagccccat cgcgggtgat tcgaaccaac
     3841 agacgatgac gacaccggtg agcaggactc ctgcggcgat cagcctgacc aggaagcgcg
     3901 agatgaactc cgagatggcg gtcacgtcgc cgtcgacacg ctcgatcatc tccccgggcg
     3961 tggtgtcgtt gtggaacccc atgtcgagtg agaagagatg agaggtcagc tcctcgcgca
     4021 gttggttggt ggcgctccaa ccgatgtccg tgcccagata ggaggcgccg gcatccagca
     4081 actggttgag gatcccggcg acgaggtagg cgattcccgc cgtggtgaga ccggcgacgg
     4141 ccgccccggc catggccgag tcgatgaagt cccgcaggat gagggggatc accaactgga
     4201 ggccgatcga cccgaggagc aggagcatca gcacgatgac ccggccccgg tgtggccgga
     4261 gataggtcga gagcgtggcg cgataggacg gagccgtggc agtcatgggc ggtgacgcac
     4321 ggtatcgccg tgtgtctcct ccccgggatg aatttgggcc ctagggggcc gcggagacct
     4381 caacggtctc catgcggaga acgttccacg acagcggggg caacaccacc tcgaacgacg
     4441 cgccgtccgt gctcggcaag gggccgatcc tcggtgtgac gcggtcaggt tggtctctgg
     4501 tgttggcggc atcaggatcg tcgtctgcca acaggacgtg ctgaaccagt cgatacccgg
     4561 gaaaggcgcg catccgtacg gtgagccgga cttgctcttc tcgatggcgg ttcactgcga
     4621 acacgaccac ccggcccgcg tcgcggtcat gggttgccac tccatcgatg atcggcgtgt
     4681 caccgtacct cgagctctcg taactcaggg tggagatcag gggatcgagg acttcgcctc
     4741 tggcgaactg agaggcgtgg gcgaagggga agaaggtcgt ttgccgccag gccgggccac
     4801 caggctcggt gcgtatcggg gcgatggcgt tgaccagttg tgcctgacat gcggccgtca
     4861 cccgatcgct gtggcgaagc agcgtgatga gcagcgtgcc gaccaccact gcgtccttga
     4921 tcgagtaggt gtcctcaagc aggggagggg cgaccggcca gtcggtgggt ggtccctcac
     4981 cttggaagcg actgatgtac cagacattcc attcgtcgaa tgacaggttg atcctcttcc
     5041 gggaacgggt cctggccctg acctggtcgg ccgtcgcgac gaccgattcg atgaaggcct
     5101 ccatgtccag cgcagaagcc aagaagctgg ccgtgtctcc gtcgtgctcc tcgtagtagg
     5161 cgtgcaacga gatgtagtcg atctcgtcgt aggtgtgttc gagcaccgtt gcttcccaag
     5221 agccaaaggt cggcatcccc gagtgtgagc tgccacagac gaccagttcg aggtctgggt
     5281 cgatccttcg catcgcccgc gctgtttccg cggcaagccg cccatactcc tcggcggttt
     5341 tgtgcccgat ctgccacggg ccgtccagct cgttgcccag gcaccagagc ctcacgccga
     5401 acggctccgg gtggccgtgg ctgatacgca ggtccgacca tcgcgtccca ccgggttggt
     5461 tgcaatattc gagtagacct acagcactct ccgctccccg cgtgccgagg ttcagcgcgc
     5521 acatcatctc gacatcggcc ttccctgccc acctggcgaa ctcggcgatt ccgaattcgt
     5581 tcgtctccag cgagtgccaa gcctgatcgc gccgagccgg gcgctggtcg accgggccga
     5641 tcccgtcctc ccagctatag ccggagacga agttgcctcc cgggtagcgc accacggtca
     5701 cgcccaactc cctgaccagg tcgagcacgt cccggcgcag cccatcctcg tcggccgtcg
     5761 cgtggccgtg ttcgaagatc cccgtgtaga cgcaccgacc caggtgctcg acgaaggagc
     5821 cgaatgtccg gcgctggacg gcgccaaccc tgaattcggg atcgagagtc agcgaagcct
     5881 gcagcatcgg ggccctagcg gggcagggat gggtgcggtt gcatttgagt tgcgattttg
     5941 ctcaggaacg ttgtttgact ccggctttca gcagctccac ggtctcggcg atgcgagccg
     6001 ccctcaactc ggggcttcgc ttggtcgagt cgatccatcg caggtatgcg ttccgataga
     6061 actgggcgag cgagtcgaag aaggtgccgg catcgggctc ggcctcgagg gcggcggcga
     6121 agtcgggggc caggtcgccg cgttggggcc atgctcagtc cgtctcgtca ggctcgcgca
     6181 ccgggaggcc acggcaacag cagcgcgggt gcagtgcgca gggcttcccg atgaccccgc
     6241 gatcgacgag gcacccgc
//
LOCUS       DASTXO010000186         2038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1632256, whole genome shotgun sequence.
ACCESSION   DASTXO010000186 DASTXO010000000
VERSION     DASTXO010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632256"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /gene="metX"
                     /locus_tag="VFU96_03945"
     CDS             <1..1168
                     /gene="metX"
                     /locus_tag="VFU96_03945"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012628274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEU5112444.1"
                     /translation="KPKQLRHRGRVCETVAPNPRQSQLQYPLSSETKFYNRPGVFHLE
                     NGETLEDVQVGYRTWGEPRSEATLICHALTGSADADEWWEGLFGPNRTFDPDHDFVIA
                     SNVLGGCYGTTGPVSLRPGTDRWYGRDFPRITIRDMVDLQAGLLDHLGVETLGLVIGG
                     SMGGMQALEWAAMYPEKVGGGVAIGVGAHHSAWSVAFSEAQRAAIWADPKFLEGAYEP
                     GQGPENGLAVARMIAMISYRGKTNFESRFGRKTLEDEFEIQSYLRYQGQKLVGRFDAN
                     TYLRLVDAMDSHDLARGRGDMDQVLAGLKTPFLTVGISSDILYPATEVEELAAGLPNA
                     RYQMLHAPQGHDSFLIDMANLDGIVSRFKHDLARGITPADDFATGAGRGSSWAF"
     gene            1192..>2038
                     /locus_tag="VFU96_03950"
     CDS             1192..>2038
                     /locus_tag="VFU96_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU5112445.1"
                     /translation="MSVLPSRLLPETSKIDSDGRLSVGGCDVSDLASEFGTPLFVYDE
                     AHLRARCREAVAAFPDGVAYATKAFLCGAMARLAHDEGLMLDVATGGEIHVSLASSVP
                     AHRLVFHGNNKSPNELRKALEAGVGRIVIDSLVEMDRIEALVAAGAPRPTVLIRVNPG
                     IEAHTHEYLRTGGADSKFGITTEGGEAERAVARARASSAMDLIGIHAHVGSQVFDVAS
                     FERAIGVIAGFAAPLDIEELSIGGGLGVAYVEGETAPTITEWGKAVNDVAANSGVQAR
                     IMAEPG"
BASE COUNT          363 a          656 c          667 g          352 t
ORIGIN      
        1 aaagcccaaa cagttgagac atcgaggccg ggtatgtgag actgtggctc ccaatccacg
       61 gcagtcccaa ttgcagtatc cactctcgag cgaaacgaag ttctacaacc gtcccggtgt
      121 cttccatctg gagaatggcg agacgctcga ggacgtccag gtgggttatc gaacatgggg
      181 tgagccccgc tccgaggcca cgctgatctg tcacgccctg accgggagcg ccgacgccga
      241 cgaatggtgg gagggcctgt tcggcccgaa ccgcaccttt gacccggacc acgacttcgt
      301 gatcgccagc aatgttctcg gagggtgtta cgggaccacc ggacctgtct cgctgcggcc
      361 cggcacggac cgctggtacg ggcgtgactt ccccaggatc acgattcggg acatggtcga
      421 tctccaggcc ggcctgctcg atcacctggg agtggagacc ctcgggctgg tgatcggcgg
      481 ctcgatggga ggcatgcagg cgttggaatg ggccgcgatg tacccggaga aggtcggcgg
      541 tggtgtggcc atcggcgtcg gtgcccacca ttcggcgtgg agtgtcgcct tctccgaggc
      601 gcagcgggcc gcgatatggg cggacccgaa attcctcgaa ggcgcctacg agccggggca
      661 gggacccgag aacggtctcg ccgtggcccg catgatcgcc atgatctcgt atcggggaaa
      721 gaccaacttc gagtcccgat tcggccggaa gacgctggaa gacgagttcg agatccagtc
      781 ctacctccgg tatcagggcc agaaactcgt gggccgcttc gatgccaaca cctatctccg
      841 cctggtcgac gcgatggaca gccatgacct ggcacggggc cgcggcgaca tggaccaggt
      901 cctggccggg ttgaagacac ccttcctcac cgtcggtatc tcgagtgaca tcctctaccc
      961 ggctaccgag gtcgaagagc tcgccgcggg cctccccaac gcccggtatc agatgctgca
     1021 cgccccacag ggacacgatt ccttcctcat cgacatggcc aatctcgacg gcatagtgag
     1081 ccgcttcaag cacgatctgg cccgtgggat caccccggcc gacgacttcg cgaccggagc
     1141 cgggcgggga tcctcctggg ccttctagtt gacgggtaga cggccgcagg gatgagcgtc
     1201 ctcccgtctc gcctgctccc tgagacctca aagatcgact ccgacggacg gctgagcgtc
     1261 ggcggctgcg acgtatcgga cctggcgtcc gagttcggaa caccgctctt cgtctatgac
     1321 gaggcacatc tccgcgcccg ctgccgcgag gcggtggctg ccttccccga cggtgtcgcc
     1381 tatgcgacga aggccttcct gtgcggggcg atggcccgtc tcgcccacga cgagggcctc
     1441 atgctcgatg tcgcaaccgg tggtgagatc catgtctcgc tcgcgtcaag tgtccccgcc
     1501 catcggctcg ttttccacgg gaacaacaag tcgcccaatg agctgaggaa ggcactcgag
     1561 gccggagtcg ggcgcatcgt catcgattca ctggtcgaga tggatcggat cgaggctctc
     1621 gtcgccgcgg gggcgccgcg cccgaccgtg ttgatcagag tgaatcccgg catcgaggcc
     1681 cacactcacg aatatctccg aactggtggc gccgactcca agttcgggat caccaccgaa
     1741 ggtggggagg cagagcgcgc cgtggcgcgc gcccgtgcct catcggccat ggacctgatc
     1801 ggcatccatg cccacgtcgg cagtcaagtg ttcgacgtcg cctccttcga gagggcaatc
     1861 ggagtcatcg ccggtttcgc cgcacccctc gacatcgagg agctctcgat cggcgggggc
     1921 ctgggtgtgg cctatgtgga aggtgagacg gcgccgacga tcacggaatg ggggaaagca
     1981 gtcaacgatg tcgctgccaa ctccggcgtg caggcgcgaa tcatggcgga gccgggtc
//
LOCUS       DASTXO010000187         4360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1005596, whole genome shotgun sequence.
ACCESSION   DASTXO010000187 DASTXO010000000
VERSION     DASTXO010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005596"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VFU96_03955"
     CDS             <1..405
                     /locus_tag="VFU96_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5112446.1"
                     /translation="PWAVLVALIALAAPPMFTNTVAGIQAVEPATVESARGMGLRERE
                     VLTGVELPLSMPLMMEGLRIAFVQVIATATLGALVAWGGLGRYIVDGFARQDMGRLIV
                     GAVLVGMLAIAAELGLSALQRATTPRGTKPAR"
     gene            494..1420
                     /locus_tag="VFU96_03960"
     CDS             494..1420
                     /locus_tag="VFU96_03960"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5112447.1"
                     /translation="MRRRFRARAVGAILGLALVATACGSGDGAEDDTAPEGDPIRIAS
                     FNFNESVIVAEIYAQGLEANGYTVERQLNLGNREVVKPALEGGDIDLVPEYTGSVGGF
                     LEVEQSADEDTMWESARTAFEELGVTLLAYSPAQDKNAYVVTGETAEEFGLTTVSDLQ
                     PVAGELVFGGPPECPERPLCLPGLQEVYGLEFAEFVPLDAGGPITVAALEGGEIDVGL
                     LFTTSGVIAANEWVSLEDDMGLNPAENVVPAIRTEVVDAYGDDLVALLDSYSEALTTE
                     DLTAMNARADLEQEDPALIAEEWLNENGFIEG"
     gene            1482..1670
                     /locus_tag="VFU96_03965"
     CDS             1482..1670
                     /locus_tag="VFU96_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003915606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112448.1"
                     /translation="MLIDCEQCAMQGTSACDDCVVTFLLSDGPVDLDESEVNALANLA
                     GEGLVPRLRLVPTERRAG"
     gene            1720..2031
                     /locus_tag="VFU96_03970"
     CDS             1720..2031
                     /locus_tag="VFU96_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112449.1"
                     /translation="MVKFYSIALALGVIGLVVVILGGAFAENVDREERDPGRRIGRGG
                     RAAIGALVGFGMAGLSAEFSTLDLAWQTALLIAVAGAAAGALWAWYASRHESGSDAGP
                     V"
     gene            2000..2788
                     /locus_tag="VFU96_03975"
     CDS             2000..2788
                     /locus_tag="VFU96_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU5112450.1"
                     /translation="MSRDPMLVLSDIHFAIEPLRRVVASGETLLILGDLVNLTDYRTG
                     EGAVADVLGMEFARASASARGAGDYQRMRGLWLETVGDRIDEIRAAIGEAIEAQYARV
                     SEALEGGHGFVIHGNVDRPGPLEQALPPGYEYVHGETRELEGLRFGFVGGGAATPLQA
                     EGEVTDDEMRRLLEALGEVDVLCTHVAPALDPLQTDVVTGRAERGSAPVLDYIRANRP
                     RLHLFGDVHQPQASRWRVGPTRCRNVGYFRATERAIAFHPADLG"
     gene            2821..3261
                     /locus_tag="VFU96_03980"
     CDS             2821..3261
                     /locus_tag="VFU96_03980"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU5112451.1"
                     /translation="MTEGTFQTLEIDAPAEALYDVAADIASYPEWATGVKEVEVLESN
                     SEGLVERARFVLEGFVKEIEYVLRYTHDRPNVLSWVAEEDSKDVRMLEGSYTFNDVDG
                     ATEVVYALTVEPNFTIPGFLRRQAEKQIVTTALRGLRKRVTGAV"
     gene            3264..>4360
                     /locus_tag="VFU96_03985"
     CDS             3264..>4360
                     /locus_tag="VFU96_03985"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HEU5112452.1"
                     /translation="MRIVLVTGKGGVGKTTVAASTALRAADSGHRTLITSTDPAHSLA
                     DALGVALGSEASQVVPNLDGQQIDTQRQLDRYWGSIRRQLMDVLDWGGAGGIEAEEFL
                     VFPGMDELFALLEVNRHARSGSYDVIVVDCAPTAETLRLLSLPEVLSWYFEKVLPTER
                     RLMKAARPILSRLTDLPLPQDEVFTAAQSVFESIEGVKELMSDPGVTSARLVVNPEKM
                     VIDEARRTYSYLGLFGYGVDGVIVNRVLPEQVADPYFERWRAIQKGHLDAVEDAFAEV
                     PRLHLRLFDDEMVGIDRLRFMAGELYGQRDPILDFTATQPFRIIEVDGGVAMEMDIPF
                     VDKTDLDVYRHGHELYIQVGPYRRSFILPDAL"
BASE COUNT          764 a         1322 c         1476 g          798 t
ORIGIN      
        1 ccctgggcgg tcctggtggc gctgatcgct ctcgccgccc ctccgatgtt caccaacacc
       61 gtggccggga tccaggcggt ggaacccgca accgtcgagt ccgcacgcgg catgggcttg
      121 agggagcgag aggtgctgac cggtgtcgag ctcccacttt cgatgccgtt gatgatggag
      181 ggactgcgaa tcgcgttcgt gcaagtcatc gccacagcca ccctgggcgc cctcgtcgcc
      241 tgggggggcc tggggaggta catcgtcgac gggtttgcca gacaggacat gggacgcttg
      301 atcgtggggg cagtcctcgt cggcatgctc gccatcgcag ccgagctggg tctttccgcc
      361 ttgcagaggg cgacaacccc gcgaggtacc aaacccgccc gctgagggga atctggcgta
      421 tcggtctacg gtttgccggg tgggccctgg tggaaagaca gggcctgttt ccaacttaga
      481 gaaggagata cccatgcgga gacgattccg cgctcgcgcc gttggggcga tcctcggtct
      541 ggcactcgtt gccacggcct gtgggtcggg tgacggcgcc gaagatgaca cagccccgga
      601 gggcgacccg atacggatcg cgtcgttcaa cttcaacgaa tcggtgatcg tggcagagat
      661 ctacgcccaa ggcctggagg cgaacgggta cacggtggag cgccagctca acctcggcaa
      721 ccgtgaggtc gtcaaaccgg ccttggaggg aggcgacatc gatctcgtcc ccgagtacac
      781 gggatctgtg ggcggattcc tcgaggtcga gcagtcggcc gacgaggaca ccatgtggga
      841 gtcggcacgg actgcgttcg aggagctggg agtgaccctg ctcgcctaca gcccggcgca
      901 agacaagaac gcatatgtgg tgacggggga gactgccgag gagttcggtt tgacgacggt
      961 gtccgatctc cagccggtgg ccggtgagct ggtcttcggc ggtccgccgg agtgccccga
     1021 acgtccgctg tgcctgccgg gacttcagga ggtctacggg ctcgagttcg ccgagttcgt
     1081 gcctctcgac gccggtggcc cgatcacggt tgccgccctg gagggtgggg agatcgacgt
     1141 gggcctcctc ttcaccacat cgggcgtcat tgccgccaac gaatgggtct cgctggagga
     1201 cgacatggga ctgaacccgg ccgaaaacgt cgtgccggct atacgcactg aagtggtcga
     1261 cgcctacggc gacgacctcg tggcgctgct cgactcgtac tcggaggcgc tcacgaccga
     1321 ggatctcact gcgatgaacg ccagggccga cctcgaacag gaggacccag ccctgatcgc
     1381 cgaggagtgg ctgaacgaga acgggttcat cgaaggctga cctccactgc gggagaaatc
     1441 tgtcccaggc atctcgtacc ttcgtcaaca ggaggtacga gatgctgatc gattgcgagc
     1501 aatgtgcgat gcagggcacc agcgcctgcg acgactgcgt ggtcactttt ctcctgtcgg
     1561 acggcccggt agacctcgac gagtccgagg tcaacgcact ggccaatctg gctggggagg
     1621 gattggtgcc gcggctgcgt ctggtcccga ccgagaggcg ggcaggctga gagacgcggg
     1681 caggctgaga gacatagggc aattactctc gcccccgaca tggtcaagtt ctattcgatc
     1741 gctctcgccc tgggtgtgat cgggctcgtc gtcgtcatcc tgggcggtgc cttcgccgag
     1801 aacgtagacc gggaggagag agatccgggc cgtcggatcg gtagaggggg cagggcagcg
     1861 atcggtgctc tggtcggttt cggcatggcc gggctctcgg ccgagttctc cacactcgac
     1921 ttggcttggc agaccgctct gctcatcgcg gtcgcagggg cggccgccgg ggctctatgg
     1981 gcctggtacg cctcacgtca tgagtcggga tccgatgctg gtcctgtctg acattcactt
     2041 cgcaatcgag cccctgcgac gcgtggtggc cagcggcgag acactgctca tactcggtga
     2101 cctggtcaat ctcaccgatt accggaccgg tgaaggggcc gtggcggatg tgttgggcat
     2161 ggagttcgct cgggcgtcgg cgtcggcacg tggggcgggt gactatcagc ggatgcgagg
     2221 cctctggctg gagacggtgg gcgaccgcat cgatgagatc cgggctgcca tcggcgaggc
     2281 catcgaagcg caatacgcca gggtgtccga ggcactcgag ggagggcatg gatttgtgat
     2341 ccatggcaac gtcgatcgcc ccgggccact ggagcaggct cttcctcctg gatatgaata
     2401 cgtgcatggt gagacgcggg aactggaagg cttgcgtttt gggtttgtcg ggggtggtgc
     2461 ggccacgcct ctgcaggccg aaggtgaggt caccgacgac gaaatgcgtc ggctcctgga
     2521 ggcgctcgga gaagtggacg tcctgtgcac ccatgtcgcg cccgccctcg acccgttgca
     2581 aaccgatgtg gtgaccgggc gcgccgagcg cggctcggcc cccgtgctcg attacatccg
     2641 cgccaaccgc ccacggctgc acctgttcgg tgatgttcac caaccccagg cgagcagatg
     2701 gcgggtcggc cccacccgat gccgcaatgt gggctatttt cgggccacgg agcgggcaat
     2761 tgccttccat ccggccgatc tcggatagat cgcaggctgg ctaacctccg atcgtccacc
     2821 atgaccgaag gaacgttcca gaccctcgag atcgacgcac cagccgaagc cttgtacgac
     2881 gtcgcagcgg acattgcctc gtacccggaa tgggcaaccg gggtcaagga ggtcgaggtc
     2941 ctggagagca attcggaggg actcgtcgaa cgggcgcgct tcgtcctcga gggcttcgtc
     3001 aaggagatcg agtacgtcct tcgatacacc cacgatcgac ccaacgtgtt gagttgggtg
     3061 gccgaggagg attccaagga cgtgcgaatg ctcgaagggt cctatacgtt caacgatgtc
     3121 gacggggcca ccgaggtggt gtacgcactc accgtggagc cgaacttcac gatccccggc
     3181 tttctccgcc ggcaagccga gaagcagatc gtgaccacgg cgcttcgggg gctgcgtaag
     3241 agagtcactg gcgcggtctg agcatgagga tcgtcctcgt caccggcaag ggaggagtgg
     3301 gcaagacgac cgtcgccgcc tccaccgccc tgcgggcggc cgactcgggc catcggacgc
     3361 tcatcacctc caccgacccg gcacattcat tggcggacgc gctgggggtg gccttgggat
     3421 ccgaggcatc ccaggtcgtg cccaacctcg acggacagca gatcgacacc caacgtcagc
     3481 tcgatcgcta ctggggctcc atccgccgcc agctgatgga tgtcctcgat tggggtggcg
     3541 ccggcgggat cgaggccgag gagtttctcg tatttcccgg aatggacgag ctgttcgctc
     3601 ttctcgaggt gaatcgtcat gcccgatccg ggagctacga cgtgatcgtg gtcgattgcg
     3661 ccccgactgc ggagactctg cgcctgctct cacttcctga ggtcctgagt tggtatttcg
     3721 agaaagtcct ccccaccgag cggcggctca tgaaggcggc caggccgatc ctcagccgac
     3781 tcactgacct accccttcct caagacgagg ttttcacagc ggcgcaatcc gttttcgaat
     3841 cgatcgaagg tgtcaaggag ttgatgtcgg atccgggtgt cacgtcggca cggctggtgg
     3901 tgaaccccga gaagatggtc atcgacgagg cgcggcgcac ctacagctac ctgggcttgt
     3961 tcgggtacgg ggtggacggc gtgatcgtca accgggtctt gcccgagcag gtggccgatc
     4021 cgtacttcga gcgctggcgc gccatccaga aaggtcatct cgacgcagtg gaggacgctt
     4081 tcgcggaagt gcctcgtcta catctccggt tgttcgacga tgagatggtc ggcatcgatc
     4141 gattgcgctt catggcgggc gagctctacg ggcaacgaga cccgatcctc gacttcaccg
     4201 caacccagcc gttccgcatc atcgaggtcg atggaggtgt ggcgatggag atggatatcc
     4261 cattcgtcga caagacagat ctcgacgtct accggcacgg tcacgagctc tacatccagg
     4321 tcggccccta ccgccggtcc ttcatcctgc ccgacgccct
//
LOCUS       DASTXO010000188         2291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_490444, whole genome shotgun sequence.
ACCESSION   DASTXO010000188 DASTXO010000000
VERSION     DASTXO010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490444"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VFU96_03990"
     CDS             <1..408
                     /locus_tag="VFU96_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112453.1"
                     /translation="LSRRILELVHRGERVRAEIGGHTFSGRVVYAGADFASVDRGEDL
                     VEVVIDAAVWTVERSASGGIEQSGDMLTFRARLAEISATAEQMRMIVVDGRAIVGSVE
                     VVATDHVEVAQDGNTIVVPIREIAAVIRSTSRF"
     gene            complement(392..610)
                     /locus_tag="VFU96_03995"
     CDS             complement(392..610)
                     /locus_tag="VFU96_03995"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5112454.1"
                     /translation="MAEDYPPILDAAQVAELLGMNVQMVRRYAREGRLPAYKLPGGRT
                     FKFFRDEIYEFVRAHPVTAESDDVRSEM"
     gene            713..1090
                     /locus_tag="VFU96_04000"
     CDS             713..1090
                     /locus_tag="VFU96_04000"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5112455.1"
                     /translation="MTKRDLQSWLDRYILAWRANTTTAIEELFTDDATYRFHPYDEGD
                     ETVVGRDRIVAAWLEEPDDPGAWEATYEAWAVDGVLGVGVGTSRYLATADKPERTYYN
                     CFLMKFADDGRCSEFTEYYVRRP"
     gene            1161..1538
                     /locus_tag="VFU96_04005"
     CDS             1161..1538
                     /locus_tag="VFU96_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112456.1"
                     /translation="MLEFYLGYSMGQSSAAKAASLARSAAVADGTHHTNRIEDLNERI
                     DRLVMILRATLALLEEQGITPEKLMAKIEELDLQDGTDDDRVRENIVECPSCQSKVAP
                     GLHNCQLCGTQVRTDIGHPLGHV"
     gene            1577..>2291
                     /locus_tag="VFU96_04010"
     CDS             1577..>2291
                     /locus_tag="VFU96_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007469822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEU5112457.1"
                     /translation="MGSFDEVHGPDGVRPHYQALMNRLGEIGDAELSDRLGLLDSFFR
                     TLGITFAVTGHEGGLERTWPMDLVPRIIPSDEWDHIEAGLVQRVTVLNRFLDDLYVGG
                     QQAIHDGIIPRWLVESADGYVREAHGIPAPRGSRCVVAGIDIVRDDAGTYRVLEDNLR
                     VPSGISYVLENRVAMTRILPVGFASHIIRPVDHYGASLLRALRAIAPPNADDPTVVVL
                     TPGVANSAYFEHAFLARQMG"
BASE COUNT          410 a          752 c          718 g          411 t
ORIGIN      
        1 ctctccagac gaatcctgga gttggttcac cgtggcgaac gcgttcgcgc agagataggt
       61 ggtcacacct tttcgggacg agtcgtctac gccggggcgg acttcgccag cgtcgaccgg
      121 ggggaggatc tggtcgaagt cgtgatcgat gcggcggttt ggacggtgga gagaagcgcg
      181 tctggaggca tcgagcagag cggggacatg ctcacgttcc gtgcccggct ggcagagatc
      241 tcggcgacgg ccgaacagat gcgcatgatc gtcgtcgatg gaagggccat cgtcggctcg
      301 gtcgaagtgg tcgctaccga ccacgtggag gtggcacagg acgggaacac gatcgtggtc
      361 ccaatccgcg agatcgccgc cgttatccgc tctacatctc gcttctgaca tcgtcgctct
      421 ccgcagtgac cgggtgggcc cggacgaact cgtagatctc gtcccggaag aatttgaagg
      481 tccggccacc ggggagcttg taggcgggca accgaccctc cctggcgtag cgccgaacca
      541 tctgcacgtt cattcccagc aactccgcca cctgcgccgc gtcgaggatc ggggggtagt
      601 cctcagccat ctgtcgtcct cttcgttact cgaggtgagc gtacggcatc cggcgttggg
      661 ttggaatggg gttcggctcg gcctcttcaa tagagccgcg tacgatcgac caatgaccaa
      721 gcgcgatctc cagagctggc tcgaccgcta catcctggcg tggcgggcga acacgacgac
      781 agccatcgag gagcttttca ccgatgacgc cacctatcgc ttccaccctt atgacgaagg
      841 tgatgagacg gtggtcggcc gggatcggat tgtcgccgcc tggctcgagg aaccggacga
      901 cccgggcgcc tgggaggcca cctatgaggc gtgggcggtg gacggcgttc tgggcgtggg
      961 tgttggaacc agtcgatatc tggcgacggc cgacaagccc gagcgcacgt attacaactg
     1021 cttcctgatg aagttcgctg acgacggacg ctgctcggag ttcaccgagt actacgtccg
     1081 gcggccctga gggtcggcag ccagactctc tttcgatcca gccgcttcac caaacgccaa
     1141 tttccgccga tatctggacc atgctcgagt tctatctcgg ttacagcatg ggccagtctt
     1201 cggccgccaa ggccgcctcg ctggcgcgga gcgccgcagt cgccgatggg actcatcaca
     1261 ccaaccggat cgaggatctg aacgagcgga tcgatcgtct ggtgatgatc ttgcgggcca
     1321 cgctcgccct gctcgaagag caggggatca cccccgagaa actgatggcg aagatcgaag
     1381 agctcgacct ccaggacggc accgatgacg accgggtgcg agagaacatc gtcgagtgcc
     1441 catcatgtca gtcgaaggtg gcgcccggtc tccacaattg ccagctgtgc gggacccagg
     1501 tgaggaccga catcggtcat ccgctcggcc acgtctgaga aggggcacct cccgccctca
     1561 caaatagact ccgccgatgg gcagcttcga cgaggtccac ggccccgacg gggtccgtcc
     1621 ccactaccag gcgctgatga accgcctcgg cgagatcggt gacgccgagc tcagcgaccg
     1681 gctcggtctg ctcgactcct tcttccgcac cctcggcatc accttcgccg tcaccggtca
     1741 cgaaggcggt ctcgagcgca cctggccgat ggatctggtg ccgaggatca tcccttccga
     1801 cgagtgggac cacatcgaag ccgggctcgt ccagcgggtg accgtcctca atcgcttcct
     1861 cgacgatctc tacgtcggtg gccagcaggc gatccacgac ggcatcatcc cccgctggct
     1921 ggtcgagtct gccgacggat acgttcgcga agcccacggc atccccgctc cccgtggctc
     1981 ccgctgcgtc gtcgccggga tcgacatcgt ccgcgacgac gccggcacct atcgagtgct
     2041 cgaagacaac ctgcgagttc cgagcggcat ctcctacgtc ctggagaaca gggtggcgat
     2101 gacccggatc ctcccggtcg ggttcgccag tcacatcatc agaccagtgg accattacgg
     2161 agcctcgctt ctccgtgccc tccgtgccat cgccccaccg aatgcagacg atcccaccgt
     2221 cgtcgtgctc accccgggcg tggccaactc ggcctacttc gagcacgcct ttctagcccg
     2281 gcagatgggc a
//
LOCUS       DASTXO010000189         4468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_601013, whole genome shotgun sequence.
ACCESSION   DASTXO010000189 DASTXO010000000
VERSION     DASTXO010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601013"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VFU96_04015"
     CDS             <1..434
                     /locus_tag="VFU96_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of
                     2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at
                     position 37 in tRNAs that read codons beginning with
                     uridine from N6-(dimethylallyl)adenosine (i(6)A); Derived
                     by automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEU5112458.1"
                     /translation="PGLAVSTDVIVGFPGETEEDFESTLELVTEARFDSAFTFIFSPR
                     PGTAAATLTEQFVPDELIRERFNRLSELQDRISGEKNADLLGSTVEVLSEGPSRKNPQ
                     VATTRSRTGKVVHVAGAHRAGSFFLAEIRKAHAHHLVGSPV"
     gene            443..1327
                     /gene="miaA"
                     /locus_tag="VFU96_04020"
     CDS             443..1327
                     /gene="miaA"
                     /locus_tag="VFU96_04020"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEU5112459.1"
                     /translation="MAILGPTAAGKTETAIRLAEHFGGEVISVDSMQVYRGMDIGTAK
                     PTLLERRGIPHHLVDVADPGEEFSVARFRTLGRSVLAEATAPVLIITGGSGLHFRALV
                     DPLSFAPTDPGLRSELEATEDGLLVEELLTVDPSARSIVDLANQRRVVRALEIYRLTG
                     ETPSSRAGTALAEDVRRYVPEIEFSAGGIDPGGALEERVDLRLGRMRTSGLVDEVHSL
                     SAALGRTARNAVGYKEILEALKGEVSFDDAFARAASNTKKLARKQRTWFQRDPRIRWI
                     PWIDDPDQRVRRILETFD"
     gene            1324..2073
                     /gene="dapF"
                     /locus_tag="VFU96_04025"
     CDS             1324..2073
                     /gene="dapF"
                     /locus_tag="VFU96_04025"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEU5112460.1"
                     /translation="MSRFEFTKMQGLGNDFILLEGPAKPAAAEVVRLCDRRFGIGADG
                     VLVVSRGAPIVMDYWNADGSAAEMCGNGLRCVARYAYDKGWVDSAEFEVDTPIGIRQV
                     RVGEDLVDAEIGPTLIEGQETIDGEVYRLVDTGNPHAVRVVEDPGAIDLARIGPIVAR
                     EAGFDTGCNVEVISVTGDRVKMRVWERGVGETLACGTGMVAAAAVALDGADGTITVEV
                     LGGAGEVEITDKNAWLTGPAEYVFTGVFDQA"
     gene            complement(2064..2684)
                     /gene="lexA"
                     /locus_tag="VFU96_04030"
     CDS             complement(2064..2684)
                     /gene="lexA"
                     /locus_tag="VFU96_04030"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEU5112461.1"
                     /translation="MLTERQQQVLEYIRQTVAERGYPPSVREIGEAVGLSSPSTVHSH
                     LSALVSAGAIKRDPTKPRAIMIVAEEAVTTQAQDDRVRDIPLLGRIAAGTPILAAEHV
                     EEVLPLPVDLVGNDPVFLLEVKGDSMIGAGILDGDLVAVRSQKDARDGDIVAALIDGD
                     EATVKRLRRHEGNVILEPANPAYEPMVYSDGVELVGKVVSVLRRYA"
     gene            2871..3152
                     /locus_tag="VFU96_04035"
     CDS             2871..3152
                     /locus_tag="VFU96_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU5112462.1"
                     /translation="MQKANLQLSVRLSLLLAALCVVFLMIGGLARADAPESAPIRYVV
                     ESGDTLWGIATAHAGPDQDVRRLVADIARLSEVDPGSIFPGQVLLIPVD"
     gene            3186..3635
                     /gene="nrdR"
                     /locus_tag="VFU96_04040"
     CDS             3186..3635
                     /gene="nrdR"
                     /locus_tag="VFU96_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004116911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEU5112463.1"
                     /translation="MLCPVCGAPSTRVVDSRPAEGGQSIRRRRECESCDQRFTTYERI
                     EPQLMVRKRNGRLEPFSAAKLAAGIAAALADRPVSGSSIDRLVEEVEASFHAAGSQVS
                     SEEIGREVLERLRALDEVGYLRFASVYKDFQGAADFEKEVAALESAE"
     gene            complement(3624..>4468)
                     /locus_tag="VFU96_04045"
     CDS             complement(3624..>4468)
                     /locus_tag="VFU96_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112464.1"
                     /translation="VTDLWETTELDAPSLLDWTPQNFGTKHLGRRRFRWPMVASLGAM
                     LVVGAGFAYWLYREPTSSATAAMGQVQTEAEALSTAIDEVAPLVNGLHLERLPEANQD
                     ASVFFQMGESARAMFAASAGLPADDSANRSTAAEAAGLAIDASRQLMDATAYRTALEP
                     ALTLPLLETNPDLTDITTATDAFTEWRTGFESVRAALPSGIADQATAALATISSGLET
                     TQTAYLDAIRTDNRTAAVEVIGTLRADLLGVRQAMLTDIAAVSESVDALIDQAEADLT
                     RLLG"
BASE COUNT          789 a         1345 c         1529 g          805 t
ORIGIN      
        1 tccccggtct cgccgtgtcc acagacgtca tcgtcggatt ccccggggag accgaggagg
       61 atttcgagtc caccctcgag ctcgtgacgg aggccagatt cgacagtgcc ttcacgttca
      121 tcttctcacc gcgaccaggc acggctgccg ccacgctcac cgagcaattc gtgcccgatg
      181 agttgatccg cgagagattc aatcgtctca gcgaattgca ggatcggatc tcaggagaaa
      241 agaacgccga cctcctcggc agcacggtcg aggtgctcag cgagggcccg tctcgcaaga
      301 atccgcaagt ggcaaccacc cgcagcagga ccggcaaggt agtgcatgtg gcgggcgcac
      361 atcgcgcagg gtcgttcttc ctggccgaga tcaggaaggc ccatgcgcat catctcgtag
      421 ggagccctgt ctgacccggg tcgtggccat tctcgggcca acggcggcag gcaagaccga
      481 gacggcgatc cgcctggcgg agcatttcgg aggagaagtc atctcggtcg attcgatgca
      541 ggtctaccgg ggcatggaca tcgggacggc aaagccgaca ctgctggagc gacgggggat
      601 acctcatcat ctcgtcgacg tcgccgaccc cggcgaggag ttctcggttg cccgctttcg
      661 cacactgggg cggagcgtgt tggccgaggc gacggcaccg gtcctgatca tcacgggtgg
      721 gtcgggtttg cattttcggg cgctggtgga cccgctcagc ttcgctccga ctgatcccgg
      781 gctccgctcg gagctggagg cgacagagga cggcctgctc gtcgaggagc tgttgaccgt
      841 tgatccgtct gcccgatcga tcgtggatct cgccaaccag cgccgggtgg tgcgcgcact
      901 cgagatctac cggctcaccg gcgagacccc cagcagcagg gcgggaactg cgctggccga
      961 agacgtacgg cggtatgtgc cagagatcga gttctccgca ggtgggatcg atcctggtgg
     1021 cgccctcgag gaacgggtgg atctccgctt ggggaggatg aggacctcgg gtttggtcga
     1081 cgaagtccac tcgttgtcgg cggcgctggg caggacggcg agaaatgcgg tcgggtacaa
     1141 ggagatcctg gaggcactaa agggtgaggt gagcttcgac gatgcgttcg caagggctgc
     1201 gtcgaatacc aagaagctcg ccaggaagca gcgcacctgg tttcagcgtg atccgcggat
     1261 acgctggata ccgtggatcg acgatcctga ccagcgggtg cggcggattt tggagacttt
     1321 cgattgagcc ggttcgagtt caccaaaatg cagggtctgg gcaacgattt catcctgctc
     1381 gaagggcctg ccaaacctgc agccgctgag gtggtccggc tctgcgaccg ccggttcggg
     1441 atcggggccg acggcgtgct cgtggtatcg cggggagctc caatcgtcat ggactattgg
     1501 aatgccgatg ggagcgcggc cgagatgtgc gggaacggct tgcgctgtgt tgcccgctat
     1561 gcctacgaca agggttgggt cgacagcgcc gagttcgagg tggacacacc gatcgggatc
     1621 cgacaggtgc gggtgggtga ggacctggtc gatgccgaga tcgggcccac tctcatcgaa
     1681 ggtcaggaga ccatcgacgg cgaggtctac cggttggtgg acaccgggaa tccccacgcg
     1741 gtgagggtgg tggaggatcc cggggccatc gatctggctc ggatcgggcc catcgtggcc
     1801 cgggaggcgg gattcgacac cggttgcaat gtcgaagtga tctcggtcac gggcgatcga
     1861 gtgaagatga gggtttggga gcgtggtgtg ggtgagacgc tcgcctgcgg taccgggatg
     1921 gtcgccgctg cagccgtcgc acttgatggc gcggacggga cgatcaccgt cgaggtcctc
     1981 ggcggggcag gtgaggtgga gatcacagac aagaacgcct ggctgaccgg tccggccgag
     2041 tacgtcttca ccggggtatt cgatcaggca tagcggcgga ggacactcac cactttcccc
     2101 accagctcga caccatcgct gtagaccatg ggctcgtagg cgggattcgc cggctcgagg
     2161 atgacgttgc cctcgtgacg ccgaagtctc ttgaccgtgg cttcgtcgcc atcgatcaac
     2221 gcggcgacga tatcgccatc gcgggcgtct ttctggctac ggaccgcgac caggtcgccg
     2281 tcgaggatgc cggctccgat catcgaatca cccttgacct cgagaaggaa gaccgggtcg
     2341 tttccaacta ggtcgacagg cagcggcagg acctcttcga catgctcggc ggccaggatt
     2401 ggcgtcccgg cagcgatgcg accgagaaga ggaatgtcgc gaacgcgatc gtcctgggcc
     2461 tgggtcgtca cggcctcttc agcgacgatc atgatcgccc tcggcttggt gggatctcgt
     2521 ttgattgccc cagcgctcac caatgccgac agatgcgaat gcaccgtcga tggcgacgaa
     2581 aggcccacgg cttcgccgat ctctctcacc gaggggggat agccacgctc ggccaccgtc
     2641 tgacgtatgt attcgaggac ctgctgctga cgttccgtga gcaccgcgac tccttccggg
     2701 aggtctccac cgactccgac tcgaccaatc taatcgaaca agagttcgcc gccaaacatc
     2761 tgttcgattc gtccttgaca tcgaacgtct gttcgataga ttgacgaacg catgttcggt
     2821 tacgaactgc atccagcaac ccgcaagggc ccgatcagga ggaagaccga atgcagaagg
     2881 caaacctgca gctgtccgtc cggctgtcat tgcttctcgc agccctctgt gtggtcttcc
     2941 tcatgatcgg cggtttggcc cgggccgatg cccccgagtc ggcgccgatc agatacgtcg
     3001 tcgaatcggg tgacacgctg tggggcatcg caaccgccca tgcggggccc gatcaggatg
     3061 tgcggcgtct ggtagccgat atcgcccggt tgagtgaggt cgatccaggc tcgatcttcc
     3121 cgggtcaggt gctgttgatc ccggtcgact gagccctacc catcattggc gtttaccctg
     3181 cgcacatgct ttgcccggtc tgtggcgctc ccagcacccg tgtcgtcgac agccgtcccg
     3241 ccgagggtgg ccagtcgatc cgacgacgac gcgagtgcga gagctgcgac cagcgattca
     3301 ccacctacga gcggatcgag ccccagctga tggtgcggaa gagaaacggt cggctggagc
     3361 cgttctccgc cgccaagctg gcggccggga tagcggcggc cctcgccgac cgaccggtgt
     3421 ccggatcgtc gatcgaccga ctcgtcgagg aagtcgaggc gtctttccac gccgcgggat
     3481 cgcaggtgag ctccgaggag atcggccggg aggttctcga gcgccttcgc gccctcgacg
     3541 aagtgggata tctgcgcttc gcctcggtct acaaggactt tcagggggca gcggacttcg
     3601 agaaggaggt cgccgccctg gaatcagccg agtaggcggg tcaggtcggc ctcggcctga
     3661 tcgatgaggg cgtcgaccga ctcggaaacg gcggcgatat cggtgagcat cgcctgtctc
     3721 acacccagga ggtccgcccg gagtgtgccg atcacctcca ctgccgcggt cctgttgtcc
     3781 gtgcggatgg cgtccaggta tgcagtctgc gtggtctcga gccccgagct gatcgtcgcc
     3841 aacgccgccg tcgcctgatc ggcgattccg gaagggagcg cggcccggac cgattcgaaa
     3901 ccggtgcgcc attcggtgaa ggcgtcggtc gccgtcgtga tgtcggtcag gtccgggttt
     3961 gtctccagca gcggcagcgt cagcgccggc tccaaagcgg tgcggtacgc ggtggcgtcc
     4021 atgagttgac gggaagcgtc gatggcgaga cccgctgctt cggcggccgt ggatcggttg
     4081 gcggagtcat cggccggcaa accggcggag gcggcgaaca tcgcccgggc gctctctccc
     4141 atctggaaga acacggatgc gtcctgattg gcctcaggaa ggcgctcgag atggagaccg
     4201 ttgacgagtg gagcgacctc atcgatcgcc gtggaaagag cctcggcctc ggtctgtacc
     4261 tgacccatgg cggcggtggc ggagctggtc ggctctcgat agagccagta ggcaaaccca
     4321 gcgccgacga cgagcatggc tccgagtgag gcgaccatcg gccagcggaa acgccgccgg
     4381 cccaggtgct tcgtgccgaa attctgcggt gtccagtcga gcagcgaagg agcgtcgagc
     4441 tcggtggtct cccataggtc ggtcacca
//
LOCUS       DASTXO010000190         4751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1448603, whole genome shotgun sequence.
ACCESSION   DASTXO010000190 DASTXO010000000
VERSION     DASTXO010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448603"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VFU96_04050"
     CDS             complement(<1..326)
                     /locus_tag="VFU96_04050"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEU5112465.1"
                     /translation="MSRDPSASTLTMIGRALRRRCPRCGAKAFTSYFTLHEHCPGCGL
                     RFEREEGYWVGALVINTAVVFGSFLVLFVGGMLISWPDVPWGALAVVTLGTMALLPIL
                     FYPWSKT"
     gene            351..995
                     /gene="pdxH"
                     /locus_tag="VFU96_04055"
     CDS             351..995
                     /gene="pdxH"
                     /locus_tag="VFU96_04055"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEU5112466.1"
                     /translation="MIEDQVAQLRREYEAMGLSEGDMAADPITEFRSWFDGILDAGLE
                     EPNAFVLATADADARPSARAVLMKGIDDEGLVFYTNLASRKSEELRVNPRAAATFVWI
                     PLHRQVRFEGRMTMVTGEDADLYFDARPRGAQIAAHASPQSQIVASREELDRAFEELD
                     VKFKGVKVPRPRAWGGWRLSPESVEFWQGQPNRFHDRIRYVRDGAGWRKERLAP"
     gene            992..1732
                     /locus_tag="VFU96_04060"
     CDS             992..1732
                     /locus_tag="VFU96_04060"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5112467.1"
                     /translation="MKVNVIGSSGTFPVAGRPASGYVVEQGGTRVWLDAGPGTFVNLP
                     VDSYMIDAMVISHQHPDHSCDIFTAFHAWTYCPDPRRNVPLYAPQEVWDRVSGYLDGG
                     QGTRLTETFDFTPVWTGDRVEIGDFSVSFVEMDHSVPTVGSRWEADNRTLFYTGDTGP
                     KGEWRESARDVDFMLCEAAYQERGEGDYPHHLTSTEAGQIAREVGARSLALTHIPPYL
                     DVSRSIDEAEKAFDRPVMLAMPGVSFDV"
     gene            1725..2429
                     /gene="rph"
                     /locus_tag="VFU96_04065"
     CDS             1725..2429
                     /gene="rph"
                     /locus_tag="VFU96_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEU5112468.1"
                     /translation="MSERPVDQLRDVKLEIPFTSSTPGSVLISMGKTKVLCTASVQSD
                     VPRWMRESGKGWITAEYAMLPGSSPERVRRDHVSGGRAKEISRLIGRSLRAVTDLKAM
                     GEAMVMVDCDVIEADGGTRTAAITGAWIALHEALADAVERGEMTSVPVIDEVAAVSVG
                     IVEGTPMLDLDYEKDVEAEVDMNVVMSGRGLLVEVQGTAERAPFTREELDRLLDLAAA
                     GIAELHDLQRRAVGAE"
     gene            2426..3007
                     /gene="rdgB"
                     /locus_tag="VFU96_04070"
     CDS             2426..3007
                     /gene="rdgB"
                     /locus_tag="VFU96_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018383950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEU5112469.1"
                     /translation="MIPRLVVASKNPDKVSEIEQVLDELGLAGEIVRGLDWPDVEESG
                     ETLEENALLKAHAVMEAVGLPALADDTGLEVDSLGGLPGVRTARFAGAGATYQDNVDL
                     LLQQMEGVEDRRAQFRTAVALVMPDGSEIVVDGLLDGVIADYPRGDGGFGYDPVFEVG
                     NRTLSEMGVAQKNTLSHRARAIRALAEALGIHR"
     gene            3004..4263
                     /locus_tag="VFU96_04075"
     CDS             3004..4263
                     /locus_tag="VFU96_04075"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5112470.1"
                     /translation="MTHEPLRVVIVGAGFAGLNAAKALRQEAVHITIVDRRNFHLFQP
                     LLYQVAAAALNPSDIAYPIRSIFRRQENVRSVLLAEVIGVDLDGKTVTLDDGSLLPYD
                     YLILATGATHSYFGHEDWERGAPGLKTLEDALVMRQRILAAFEQAERNPEDASRLLTF
                     VVVGGGPTGVELAGALIEIAVHSLGDEFDSIEPARARVILVEGAPHVLPVYPTDLSES
                     AEHQLKSLGVEVLTGTMVSEIDDAGVSLSDGRRIDSALVLWAAGVQASELGQMLGVET
                     DRSGRVPVEGDLSVPGHREVFVTGDLATVPGVPGVAPAAMQMGTHAGRMIEGDLRTRS
                     RISFHYRKKGALATIGRARAVADFGRVRFGGFLAWVAWLAIHIFYLIGFRNRVLVLIS
                     WAWSYLTFRRGARIITGVPPLPEKGEQ"
     gene            complement(4298..4648)
                     /locus_tag="VFU96_04080"
     CDS             complement(4298..4648)
                     /locus_tag="VFU96_04080"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5112471.1"
                     /translation="MKLEFLYAPTSDLNASIALYRDGLGFSEVWREGETTVALSLPGT
                     DVQLMLDANDPDAPFGPLFVVDSVEAFHAARPEPLTVIEEISEIPGGLLATYQDPGGA
                     TIYVMDQSTDSAIQ"
BASE COUNT          856 a         1384 c         1629 g          882 t
ORIGIN      
        1 gtcttggacc atgggtagaa gagaatcggg aggagggcca tggtccccaa tgtcaccacg
       61 gccaacgcac cccacggcac atctggccag gagatgagca tcccgccgac gaagagcact
      121 aggaacgacc cgaatacgac ggcggtgttg atgaccaggg ctcccaccca atatccctcc
      181 tcgcgctcga atcggagacc gcaacccggg cagtgctcgt gcagggtgaa gtacgaggtg
      241 aacgccttgg ctccgcatcg cgggcagcgc cgtcgcaacg cccgcccgat catggtgaga
      301 gtcgatgcgc tcgggtctcg gctcactggc ccgaggctat cgtcagccgg gtgatcgagg
      361 atcaggtggc tcagcttcgc cgcgagtacg aggcgatggg cctgagcgag ggagacatgg
      421 cagccgatcc gatcacagag ttccgatcgt ggttcgacgg catcctcgac gcggggctcg
      481 aggagccaaa cgctttcgtc ttggcaactg ccgatgccga cgcccggccg tcagcccgag
      541 ctgtcctcat gaagggaatc gacgacgagg ggctggtgtt ctacacgaat ctggccagtc
      601 ggaagagtga agagttgcgg gtgaacccgc gcgcggcagc cacgttcgtg tggattccgc
      661 tgcatcgtca ggtccgcttc gaggggagga tgacgatggt caccggtgag gatgccgact
      721 tgtatttcga tgcgagaccg agaggtgcgc aaatcgccgc tcatgcctcc ccgcagagcc
      781 agatcgtggc gtcccgtgag gagctggacc gggcattcga ggagctcgac gtcaagttca
      841 aaggtgtgaa ggtgccccgg ccccgggcat ggggcgggtg gaggctgtca ccggagagcg
      901 tggagttctg gcagggtcag ccgaaccgtt tccacgaccg gattcgatat gtccgagatg
      961 gcgccgggtg gcggaaagag aggctggcac cgtgaaggtc aacgtcatcg ggtcctccgg
     1021 gaccttccct gtcgccggcc gccccgcttc ggggtatgta gtggaacagg gcgggaccag
     1081 ggtctggctg gacgccggcc cgggcacatt tgtcaatctt cccgtcgatt cgtacatgat
     1141 cgacgccatg gtcatctctc atcagcaccc cgaccacagc tgtgacatct tcacggcatt
     1201 ccatgcctgg acctattgcc cggatccgag gaggaatgtt ccgctctacg cgccccagga
     1261 ggtgtgggat cgcgtgtcgg gctatctcga cggtgggcag ggcacgaggc ttaccgagac
     1321 tttcgatttc acgccggtct ggaccgggga tcgtgtcgag atcggtgact tctccgtctc
     1381 tttcgtcgag atggaccact cggtgccgac ggtcggatcg cgatgggagg cagacaaccg
     1441 cactctcttc tacacaggcg ataccggtcc caagggcgaa tggcgggaat cggcacgcga
     1501 tgtcgatttc atgctgtgcg aagcggccta ccaggagcgt ggtgaaggcg attacccgca
     1561 ccatctgact tcgaccgagg ctggtcagat tgcccgagag gtcggggcgc gcagtctcgc
     1621 cctcactcac atccccccat atctcgacgt gagccggtcg atcgacgagg ctgagaaggc
     1681 gttcgatcga ccggtgatgc tggctatgcc tggagtcagc ttcgatgtct gagcgaccgg
     1741 tcgatcagct cagggatgtc aagctcgaga ttccctttac gtcgtcgacc ccgggctcgg
     1801 tgctgatctc gatgggcaag accaaagtcc tttgcaccgc atcggttcag tccgacgtgc
     1861 ctcgttggat gcgggagtca ggcaaggggt ggatcaccgc cgagtacgcg atgctgcctg
     1921 gctcctcacc agagcgagtg cgccgtgatc atgtgtcagg cggccgcgcc aaggaaatct
     1981 cccggctcat cggtcggtcg ttgcgcgcag tgaccgatct caaggcgatg ggggaggcga
     2041 tggtgatggt cgattgcgac gtcatcgagg ccgacggtgg aacgaggacc gctgccatca
     2101 ctggggcatg gatcgccctt cacgaggcgt tggccgacgc ggtcgagaga ggtgaaatga
     2161 cttcagtgcc ggtcatcgac gaggtggcgg cggtatcggt gggaattgtc gaaggtaccc
     2221 cgatgctgga cctcgactac gagaaggacg tcgaagccga ggtcgacatg aatgtggtca
     2281 tgagcggccg gggtctgctg gtcgaggttc aggggactgc agagcgtgct ccgttcacta
     2341 gggaggagct cgaccggctc ctcgatctgg cggccgcggg catcgcagag cttcacgacc
     2401 tacaacgtcg ggcggtgggc gcggagtgat acctcgtctg gtggttgcct cgaagaaccc
     2461 cgacaaggtc tcggagatcg agcaggtcct cgacgagttg ggtctcgccg gagagatcgt
     2521 tcggggtctc gactggcccg acgtcgaaga gtccggagaa actctggaag agaacgccct
     2581 gctcaaggcg catgccgtga tggaagcagt ggggctgccc gctctggctg acgacaccgg
     2641 gctcgaggtc gattcccttg gcgggctgcc cggggtgagg accgctcgat tcgccggggc
     2701 aggcgcgacc taccaagaca atgtcgatct tctgctgcag cagatggagg gagtcgagga
     2761 tcgacgggcg cagttccgga ccgcagtggc gctggtcatg cccgatggat ccgagatcgt
     2821 cgtcgatgga ttgctcgacg gggtgatcgc agattacccc cgcggcgacg gcggatttgg
     2881 ctacgacccc gtgttcgagg tcgggaatcg gactctgtcc gagatgggag tggcccagaa
     2941 gaacacactg tctcatcggg cccgggccat cagggcgttg gcggaggcgc tcgggatcca
     3001 ccggtgacac acgagcctct ccgggtcgtc atcgtcggag ccgggtttgc cggtctgaat
     3061 gcagccaagg ctctacgtca ggaagcggtc cacatcacga tcgtcgatcg gcggaacttc
     3121 cacctctttc agcccctcct ctatcaggtg gctgccgctg ccttgaaccc gagtgacatc
     3181 gcctacccga tccgctcgat cttccgacga caggagaacg tgaggagcgt ccttctcgcc
     3241 gaagtgatcg gggtcgatct cgacggcaaa acggtgaccc tcgatgatgg ctcgctcctt
     3301 ccctatgact acctgatcct cgccaccggg gccacgcatt cgtacttcgg ccacgaagac
     3361 tgggagcggg gggcacccgg gttgaagact ctcgaggacg cgctggtgat gcgtcaacgg
     3421 atcctggcgg ccttcgagca ggcagagcga aaccctgagg atgcgtctcg gctgctcacc
     3481 ttcgtggtcg tcggcggggg cccaacgggc gtcgaattgg caggcgcgtt gatcgagatc
     3541 gccgtgcatt cgttgggtga cgagttcgac tcgatcgagc cggcgcgagc gcgagtgatc
     3601 ctggtggaag gagctcccca cgtgctgccg gtctatccaa cggacctgtc ggaaagcgcc
     3661 gaacatcagt tgaagagcct cggcgtcgag gtcttgaccg ggaccatggt gagcgagatc
     3721 gacgacgctg gggtctccct cagtgacggg cgtcggatcg actcggccct ggtgctctgg
     3781 gcggctggcg ttcaggcatc ggagttaggg cagatgctcg gagtggagac cgaccgaagc
     3841 ggtcgcgttc ctgtcgaggg agatttgtcg gtccccggtc atcgcgaggt tttcgtcacc
     3901 ggtgacctcg caacagtccc gggtgtgccc ggggtcgccc cggcggcgat gcaaatggga
     3961 acacacgccg gccgcatgat cgagggagac ctccggacgc gctcgagaat ctccttccac
     4021 tatcggaaaa agggggcact ggccaccatc ggccgggcga gggcagtggc tgacttcggc
     4081 cgggtcagat tcggcggatt cctggcctgg gtcgcatggc tggccattca catcttctat
     4141 ctgattgggt tccgcaaccg ggttttggtt ttgatctcct gggcctggag ctatctcacc
     4201 ttccgacgtg gggccagaat catcaccgga gtcccgcccc tgccggagaa gggcgagcaa
     4261 taggccagat gcgccgtgtt ctctccaaat cgcggggtta ctgaatcgcg gagtctgtcg
     4321 actggtccat gacgtagatg gtggccccgc ccgggtcctg gtaggtggcc aggaggccac
     4381 ccgggatctc tgagatctcc tcgatcacgg tcaggggctc cgggcgggcg gcgtggaaag
     4441 cttcgacact gtcgacgacg aagagcgggc cgaacggggc gtcggggtcg ttggcgtcga
     4501 gcatgagctg cacgtccgtg cccggcaacg acagggcgac agtcgtctcg ccctcgcgcc
     4561 agacctcgct gaagccgagg ccgtcgcgat agagggcaat cgaggcgttc aggtctgagg
     4621 ttggggcgta taggaactcg agcttcatga cgtctccttt cgacgacata actggattat
     4681 tgctgagcat aacggcatta tgtcagggtg cgcaactgga ttccggtgtc gacgtctccc
     4741 aagggaaggc t
//
LOCUS       DASTXO010000191         2247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_527624, whole genome shotgun sequence.
ACCESSION   DASTXO010000191 DASTXO010000000
VERSION     DASTXO010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527624"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /gene="folP"
                     /locus_tag="VFU96_04085"
     CDS             <1..573
                     /gene="folP"
                     /locus_tag="VFU96_04085"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU5112472.1"
                     /translation="DTSKPEVAHAAIGAGAEIVNDVTAAATEGMRDVVGESAAGVVLM
                     HMKGTPRTMQRDPTYEDVVSEVVTFLRSKADEMIEAGVDRASIAIDPGIGFGKTVIHN
                     LELIGRLEEIAKLGFPVVLGTSRKTFLGRVAGIEDPSRRDGVTAVTTALGFERGARVF
                     RVHDVVSSRAALGLAAAIVDPQQWDEWSQD"
     gene            549..1043
                     /locus_tag="VFU96_04090"
     CDS             549..1043
                     /locus_tag="VFU96_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112473.1"
                     /translation="MGRMVAGLKPRGFTWVITDRLAVSERVGGSGFQHRRVRREEEIT
                     WLVEEAGINTVVSMLPGNQNLQAYREAGIATFSVPIGPDLTTHDVDEFFATLSKATAK
                     RRARVLVHREAIDDTVGGLLGGFLVFSGLVNDPILALAVIQQILGRALGPEGRSLIPA
                     SAAS"
     gene            1040..1399
                     /gene="folB"
                     /locus_tag="VFU96_04095"
     CDS             1040..1399
                     /gene="folB"
                     /locus_tag="VFU96_04095"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEU5112474.1"
                     /translation="MTDRIEIKGIEVLARHGVLEHEKQEPQIFRIDLTLYLDLSPAGA
                     SDDLTDTVDYGKLAQVTHDLVHDESHDLIETVAHQIATTVLAEQSVDRVTVTVHKPEA
                     PIPLTFEDVAVTIDRSR"
     gene            1396..1899
                     /gene="folK"
                     /locus_tag="VFU96_04100"
     CDS             1396..1899
                     /gene="folK"
                     /locus_tag="VFU96_04100"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEU5112475.1"
                     /translation="MTRFAIALGSNQGARLDHLRRAVAEVDRLGSIEEVSAVYETEPV
                     GGPEQAPYLNAIVLLETTSLPRDLLAALQRIEAAHGRAREVRWGPRTLDLDIVAIDGD
                     SIREPDLEIPHPRAAERRFVLEPLCDVWPDAIVDDGLTARAARAAVTDQEVTMLAASW
                     LEDTPDQ"
     gene            2022..>2247
                     /locus_tag="VFU96_04105"
     CDS             2022..>2247
                     /locus_tag="VFU96_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112476.1"
                     /translation="MAKRSREHRQDIPGYNDGRPTREERKQQHRAVRHHAHQVLHMTD
                     DPDAVVVPEVRGTGDNHENHLADPQDSRRRR"
BASE COUNT          429 a          683 c          749 g          386 t
ORIGIN      
        1 gacacctcga agccggaggt ggcacacgcc gcgatcggag cgggggccga gatcgtcaac
       61 gatgtgaccg ctgccgccac ggaaggcatg agggacgtgg tgggtgagtc tgcggccgga
      121 gtcgtcctca tgcacatgaa ggggactcct cggacgatgc aacgcgaccc gacgtatgaa
      181 gacgtcgtga gcgaggtcgt cacctttctt cgctcgaaag ccgacgagat gatcgaggca
      241 ggtgtcgatc gggcatcgat cgcgatcgat ccggggatcg gcttcggcaa gaccgtcatc
      301 cacaacctgg agctgatcgg cagactcgaa gagatcgcca agttggggtt cccggtggtg
      361 cttggcacct cgcgcaagac ctttctgggg cgggttgccg gcatcgagga cccgagtcgg
      421 cgcgacgggg tcaccgcagt cacgaccgcc ctcggttttg agcgtggggc aagggttttt
      481 cgggttcacg acgtggtttc atcaagagcg gcgctgggcc ttgccgccgc tatcgtggat
      541 ccccagcaat gggacgaatg gtcgcaggac taaagccccg gggattcacc tgggtcatca
      601 ccgatcgtct cgctgtttcg gagcgggtcg gaggctccgg gttccagcat cgccgggtgc
      661 gacgcgagga ggagatcacc tggctcgtcg aggaagccgg gatcaacacg gtggtttcga
      721 tgcttcccgg aaaccaaaac ctgcaggcgt atcgtgaggc ggggatcgcg acattctcgg
      781 tgcctattgg gcctgacctc accactcacg acgtggacga gttctttgcc actctgagca
      841 aggcgactgc gaagcggcgg gcgcgggtcc tcgtccatcg cgaggcgatc gacgacaccg
      901 ttggcgggct gctcgggggg ttcctggtct tttcgggact ggtcaacgac cccatcctcg
      961 ctctggcggt gattcagcag attctgggac gtgcgctggg gcccgagggc cggtcgctga
     1021 tcccggcatc cgccgcctcg tgaccgatcg gatcgagatc aaaggcatcg aagtgctcgc
     1081 ccgtcatggg gtgctcgagc atgagaagca ggagccgcag atctttcgga tcgatctgac
     1141 cctgtatctc gacctttccc cagccggggc atccgatgac ctgaccgaca cggtggatta
     1201 cgggaaactg gcccaggtca cacacgacct ggtgcatgac gagagtcacg atctgatcga
     1261 gacggtcgcc catcagatcg cgaccacagt gctcgccgag cagtctgtcg accgggtcac
     1321 cgtgacggtg cacaagccgg aggctccgat ccctctgacc ttcgaggacg tggctgtcac
     1381 catcgatcga tcccgatgac ccggtttgcc atcgccctcg gctccaacca gggagcccgg
     1441 ctggatcatc tgcgtcgggc cgtcgcggaa gtcgaccggc tgggctctat cgaggaagtc
     1501 tccgctgtct acgaaaccga gccggttggg ggcccggaac aagctccgta cctgaacgcg
     1561 atcgtgctgc tcgagaccac gtcgctgcct cgagacctcc tggccgcact gcagcggatc
     1621 gaggcggcac acggaagggc acgggaggtg cgctgggggc cccgcaccct cgatctggac
     1681 atcgtggcga tagatgggga ctcgattcgg gagcctgatc tcgagatccc ccatccgagg
     1741 gcggccgagc gtcgctttgt cctcgagcca ctttgtgacg tctggccgga cgccattgtc
     1801 gacgacgggt tgacggctcg agcggccagg gcggctgtca ctgatcagga agtgacgatg
     1861 ctggccgcaa gctggctcga ggacacaccg gatcaatgat cggtcctccc aacctgacat
     1921 ggatggccga acccgtgcta gcttccacat atgggaagcg gccgcggccc tcctcttggt
     1981 cggacataga gcaatggtcc aggacaggag gaacgatcta gatggcaaaa cggtcgcggg
     2041 aacatcgcca ggacatcccc ggatacaacg acggccgccc tacgcgcgaa gagcgcaagc
     2101 aacagcatcg ggcggtgaga caccacgccc atcaggtgct gcacatgaca gacgaccccg
     2161 atgcagtggt cgtgccggaa gtgaggggca ccggggacaa tcacgagaat catctcgccg
     2221 acccacaaga ttcgagacgc cggagat
//
LOCUS       DASTXO010000192         2099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1227445, whole genome shotgun sequence.
ACCESSION   DASTXO010000192 DASTXO010000000
VERSION     DASTXO010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227445"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1866)
                     /locus_tag="VFU96_04110"
     CDS             complement(<1..1866)
                     /locus_tag="VFU96_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5112477.1"
                     /translation="MSTDPQRGGESSDLLDQFWAQLPFRPDDFQVEAADAVTDGQSVV
                     VTAPTGAGKTLVAEAAIHLALARGERAFYTTPIKALSNQKFSDFVIGYGPERVGLLTG
                     DNVINGDAEIVVMTTEVLRNMIYADGTRLDRVGLVILDEVHYLQDRTRGAVWEEVIIH
                     APQHLQLICLSATVSNNQEFAAWVGERRGPTRLISTDHRPVPLESMYMVRDRMGAQTL
                     HLLPTFIEREGRRRPNPRIEHMLGLERGRRRRFKSPNRIEAVERLAEEGMLPAIFFIF
                     SRAGCDAAAHRLVDAGVRLTDATQRASIREIAETRTAHLGDQDLTVLGYDRWLAGLEA
                     GVAPHHAGLVPAFKETVEELFEAGLLKVVFATETLALGINMPARTVVLETLSKFNGET
                     HELMRPGDYTQLTGRAGRRGIDVEGYGVVLHSPFVRFDSVTEVASIGSHPLRSSFRPT
                     YNMTANLVANYTQDQAEVLLEASFAAFQREGDKHAAETTIEALEHSLEKEEAAAHCDR
                     GDVAEYLAIVESAQPEGHNDRISSILGAGQVVDVPGGARDGRYVILKRLARKDGGARY
                     LVLSTSGRVSTIGYRDVVDGSAIVADLEIPLPVRPKDRRYVQDLLRTLRRVPPRES"
     gene            complement(1863..>2099)
                     /locus_tag="VFU96_04115"
     CDS             complement(1863..>2099)
                     /locus_tag="VFU96_04115"
                     /inference="COORDINATES: protein motif:HMM:NF013097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEU5112478.1"
                     /translation="LAFGLAFLFPVVLFAAAAAGIISSKQLARGRRWAILIIVIGAAL
                     ITPSGDAFSLLVLSVPLYLMYEATYWLVRLVLRK"
BASE COUNT          366 a          673 c          689 g          371 t
ORIGIN      
        1 cgactcacga ggtggcaccc ggcgcaaggt ccggaggaga tcctgcacat acctccgatc
       61 cttggggcgt accggaagcg ggatctccag gtcggcaacg atcgcggacc cgtccacgac
      121 gtcgcgatag ccgatcgttg acacccggcc cgaggtggag agaaccaggt accgggcacc
      181 gccgtccttg cgggccagcc gcttcaggat cacgtaacgg ccatcgcgag cgccaccggg
      241 aacgtcgacg acctggccgg cgccgagtat cgacgagatc cgatcgttgt ggccctcggg
      301 ctgggccgac tcgacgatcg ccaggtactc cgcgacatct cccctgtcgc agtgagccgc
      361 ggcctcctcc ttttcgaggg agtgttccaa cgcttcgatg gtcgtctcgg cggcatgttt
      421 gtcgccctcg cgctggaacg cggcgaagga agcctccaga agcacttctg cttggtcctg
      481 ggtgtagttg gcaacgagat ttgccgtcat gttgtaggtg ggacggaagc tcgagcgaag
      541 ggggtgcgac ccgatcgagg cgacctcggt gaccgagtcg aatctgacga acggcgaatg
      601 caggacgaca ccgtatccct cgacgtcgat ccctcgacgc cccgccctac ccgtgagctg
      661 ggtgtagtcc cccggtcgca tcaactcgtg ggtttcgccg ttgaacttgg acagtgtctc
      721 gagcacaacg gttcgtgccg gcatgttgat gcccaaggcc agcgtctcgg tggcgaagac
      781 gactttcagc aaccccgcct cgaacagctc ttccacggtc tccttgaagg cggggaccaa
      841 cccggcgtgg tggggggcca cacccgcctc gagccctgcg agccagcggt cgtatccgag
      901 gacggtgagg tcctgatcgc caaggtgggc cgtccgtgtc tcggctatct cccggataga
      961 cgctcgttgc gtggcgtcgg tcagtcggac gccggcatcg accagacgat gggcagcggc
     1021 gtcgcatccg gccctgctga agatgaagaa gatggcgggg agcatcccct cctcggcgag
     1081 gcgctccacg gcctcgatcc ggttcgggct cttgaaccgt cgccgccgcc cgcgctccag
     1141 acccagcatg tgctcgatcc ggggattggg acgtcttctc ccttctcgct cgatgaaggt
     1201 ggggaggagg tggagggttt gtgcccccat acggtccctg accatgtaca tcgactcgag
     1261 tggcaccggc cggtggtcgg tagagatgag ccgggtaggc ccgcgtcgct cccccaccca
     1321 tgctgcgaat tcctggttgt tggagacggt ggccgagaga cagatcaatt gcaggtgctg
     1381 cggggcatgg atgatcacct cttcccacac cgcgccacgg gtgcggtcct ggagatagtg
     1441 gacttcgtcg aggatcacca gacccaccct gtcgaggcgg gtcccgtcgg catagatcat
     1501 gttgcgaagg acctcggtgg tcatcaccac gatttcggcg tccccgttga tcacgttgtc
     1561 cccggtcagg aggcccactc gctccggccc gtacccgatg acgaaatctg agaacttctg
     1621 attggacagg gccttgatcg gcgtcgtgta gaacgcacgc tcaccgcgtg cgagcgcgag
     1681 atggatggcc gcctccgcca cgagagtctt ccccgcgccg gttggtgcgg tgacgacaac
     1741 cgattgaccg tccgttaccg cgtcggccgc ctccacctgg aagtcgtccg gtcggaacgg
     1801 gagctgcgcc cagaactggt cgagcagatc gctgctctct cccccacgct ggggatcggt
     1861 gctcatttcc tgagcaccag acggaccaac cagtaggtgg cctcgtacat cagatagagg
     1921 ggaaccgaca ggaccaggag gctgaacgcg tctccgctcg gcgtgatgag cgccgcgccg
     1981 atcacgatga tcaggattgc ccatcgccgc ccccgggcaa gctgcttgga gctgatgatc
     2041 ccggctgcgg ctgcggcgaa caggacgacg ggaaagagaa aggccagtcc gaacgcgag
//
LOCUS       DASTXO010000193         4543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_158984, whole genome shotgun sequence.
ACCESSION   DASTXO010000193 DASTXO010000000
VERSION     DASTXO010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158984"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VFU96_04120"
     CDS             <1..330
                     /locus_tag="VFU96_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase"
                     /protein_id="HEU5112479.1"
                     /translation="EDLDLGPAYTVLTSHQDQIEDLPPGAEILGWNEHCPVSMLGVGE
                     NMIGIQGHPEFDPAYSKALMESRRGGLIPEAIVDDGLASLGGHTDGQLLVDWILRRFS
                     SDANRSS"
     gene            complement(302..3730)
                     /locus_tag="VFU96_04125"
     CDS             complement(302..3730)
                     /locus_tag="VFU96_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5112480.1"
                     /translation="MICSVCNTENPANNRFCGNCGSALAITCSTCGASNEPGTNFCGQ
                     CGTSLAGSQIQLTPTVTAERRLVTVLFADLTGFTDFSEGRDAEEVRDFQTSYFDQSRR
                     VIQLFGGIIEKFIGDAVMAVWGAETATEDDAERAIRAGFELIDIVAKLSADVGAPDLG
                     LRVGIHTGEAAVGPNDDRMGFVTGDLVNTASRLQTIAEPGTVLVGESTQQATHRSIAF
                     EAVGPQSLKGKTLPVAAWQATRVLSERAGRGKAESLEPPFVGRANELRLLKDLLDAVG
                     TESRARMVSLVGEAGIGKSRLVWEFLKYIDGLVDNIYWHEGRSPAYGEGVTFWAISEM
                     IKGRAGIAEGDDDATVARRLDETLDTYVPDPGDRAWMRPRLAAVLGEGEAPGDRSELD
                     AAVRAFFEAVSDLGTTVLVFEDLHWADGALIDFVEDLTDWWRDRPILVVTMARPDLLE
                     LKPSWGAGRQGVVSITLGPMSSEDMLTLVHGAVPGLPETSAEAIVERAAGIPLYAVEL
                     LRGMLAQGQLAGEAGDYRVVGDLTQLVIPESLQAVIGARLDRLGGEEKALIQDAAVLG
                     QTFNVTGLAVLTGQDPAEIEMHLHSLSRRELIEPVRDPRAVERGQYRFLQGLIRDVAL
                     GRLGREARRTRHLAVAQYLESQDDPELAGIVAGHYLHALEASPPGEAQDAIRDRALLS
                     MAQAAGRAADLKSHQQVMLISESALEIADSDEARTPFWEMVVEASGRMADLDRAERHA
                     VQAMDHYQKTGDQVSLNRVTGTFALDLAENNQPERAVELLRPLVEDDFGTEPELVRAA
                     VVYARSLMLSMEPGVLEAADRALTATEQLGWVPETIDALITKGTSLGQLGRLSEARII
                     LEGAISLAEEHDLGRSIARGQNNLAYVLVGLDDEASLALAEEAHRTAQRLGDRSLLLF
                     QVGQSAFAYAAIGEFEKAEEVLANPLTREQPPATRVYTASAELVMAAWRGDLEEVDRL
                     EAEVEGLVDQVDDPQVEVTFKTIALEATIARGRMEEAFLLAGELLEESTWNQAADTIG
                     SPLFTAALLGDASRFATLIPLYQRYLPRFREYHATCQVLAPAIDGPVDTREIDAVIAR
                     SAGWGSVADVVRFSMIAAPFAIPEKRSEYLSNARSIATERGWHGILSLIDSHLS"
     gene            3808..4242
                     /locus_tag="VFU96_04130"
     CDS             3808..4242
                     /locus_tag="VFU96_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEU5112481.1"
                     /translation="MATKQESLELLGGVPFLSDLSKRDLNKVLAIAKPTVHADGTEVM
                     TEGRGGVGFHLITEGKAKVQRAGRTVATLGPGEFFGEMALVDDGPRSASIFADSELTT
                     LVISKWEFRPLVKSHPELAWKLIEHLVVRLREEQTGRDALVC"
     gene            complement(4239..4427)
                     /locus_tag="VFU96_04135"
     CDS             complement(4239..4427)
                     /locus_tag="VFU96_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112482.1"
                     /translation="MSYRPGEVVLSSASPAAGFSADIKKGGPPEVDVEFESESAKYRV
                     RARWEGGDLSVETESDVD"
BASE COUNT          803 a         1492 c         1429 g          819 t
ORIGIN      
        1 gaagatctcg acctgggccc cgcgtacacg gtgctcacct cgcatcagga tcagatcgag
       61 gatctacccc cgggagcgga gatcctcggc tggaacgagc attgcccggt ctcgatgctg
      121 ggtgtgggcg agaacatgat cgggatccag gggcatcccg aattcgaccc ggcctattca
      181 aaggcgctca tggagtcccg ccgcggcggt ctgattcccg aggcaatcgt tgacgatggc
      241 ttggcctccc tcggcggaca caccgacgga caactcctcg tcgattggat cctccgacgt
      301 ttcagctcag atgcgaatcg atcaagctga ggatcccatg ccaaccccgt tcggttgcga
      361 tggatcgggc gttcgagagg tattcgcttc tcttctcggg gatcgcgaac ggggcggcga
      421 tcatcgagaa cctgaccacg tcggcgactg atccccatcc cgcacttcgg gcgatgacgg
      481 cgtcgatctc gcgggtgtct accggcccgt cgatcgcagg agcaagtacc tgacacgtcg
      541 cgtgatattc gcggaagcga gggagatagc gctggtacaa cggaatcagt gtggcgaacc
      601 gtgacgcgtc gcccagtaac gccgcagtga acaggggact cccgatggtg tcggctgcct
      661 ggttccaggt cgactcctcg aggagctcgc cggcgagcaa gaacgcctct tccattctgc
      721 ccctggcgat cgtggcctcc agagcgatgg tcttgaaagt gacctcgacc tgtgggtcgt
      781 cgacctggtc gaccagtccc tcgacctccg cctcgagccg atcgacttct tccaggtctc
      841 cgcgccaagc cgccatcacc agctcagcgg atgcggtgta gacacgagtt gccggcggtt
      901 gttcccgggt gaggggattg gcgaggacct cctccgcctt ctcgaactcc ccgattgccg
      961 cgtaggcgaa cgccgactga ccaacctgga acagcaacaa ggaccgatca ccgagacgct
     1021 gcgcggttcg atgagcctct tcggccaggg caagactcgc ttcatcgtcg agaccgacga
     1081 ggacataggc aaggttgttc tgaccccggg caatggagcg gccgaggtcg tgctcctcgg
     1141 ccagcgagat ggcgccctcg agaatgatcc gggcctcgga caaccgtccc agctgcccca
     1201 gggaggttcc cttggtgatc agcgcatcga tggtctcggg cacccacccg agttgctcgg
     1261 tcgccgtcag cgcccggtcc gcagcttcga ggactcccgg ttccatgctg agcatcagac
     1321 tgcgggcata cacgactgcc gcccggacga gctccggttc tgtgccgaag tcatcttcga
     1381 cgagaggcct cagcagttcg accgctcgct cgggctggtt gttctctgcc agatccagcg
     1441 cgaaggttcc ggtcacccga ttcagcgaaa cctgatcgcc cgtcttttgg tagtgatcca
     1501 tcgcttgcac cgcatgtcgc tcggctctgt cgagatcggc catgcgccct gacgcttcga
     1561 caaccatctc ccagaacggg gtgcgcgcct cgtcgctgtc ggcgatctcc aaggcgctct
     1621 ccgagatcaa catgacctgc tgatgagact tcaagtcggc ggcgcgccct gcggcctgcg
     1681 ccatggaaag tagggctcga tcacggatcg catcctgggc ctcgcccggc ggtgacgcct
     1741 ccaaagcgtg caggtagtgc ccggcgacga tcccggccag ctccgggtca tcctgacttt
     1801 ccaggtactg cgccacggcg aggtgtcggg tgcgccgggc ctcccggccg agccggccca
     1861 acgcgacgtc ccggatcagg ccctggagga accgatactg gcctcgttcc accgcccggg
     1921 ggtcgcgtac cggctcgatc agctctcgac ggctcagtga atgcagatgc atttcgatct
     1981 cggcggggtc ctgtccggtg agaacggcca gaccggtgac gttgaatgtc tggccgagca
     2041 ccgcggcatc ctggatgagt gccttctcct caccaccgag gcggtcgagg cgtgccccga
     2101 tgacggcctg gagggattcg gggatgacca gctgcgtcag gtctccgacg acccgataat
     2161 ccccggcttc acccgccagc tgcccttgag cgagcatgcc tctcagcagc tcgaccgcat
     2221 agagggggat gccggcggcg cgctccacga tggcctccgc agaagtctcg gggagtccgg
     2281 gaacggcgcc atgcaccaag gtgagcatgt cttctgacga catcgggccg agggtgatcg
     2341 aaacgacgcc ctggcgcccg gcgccccagg acggcttgag ctcgaggagg tcggggcggg
     2401 ccatcgtcac gacgaggatc ggccgatcac gccaccaatc ggtcaggtct tccacgaagt
     2461 cgatcagcgc cccatccgcc cagtgcaggt cttcgaagac aagcaccgtg gtcccaagat
     2521 ccgacacagc ctcgaagaac gcccggaccg cggcatcgag ctcagaccgg tctcccggtg
     2581 cttcaccctc gcccagcact gctgccagtc tcggtctcat ccacgcacgg tcacccggat
     2641 cgggcacgta ggtgtcgagg gtctcatcga gtcggcgtgc gaccgtcgcg tcgtcgtcac
     2701 cctccgcgat tccagcccgt cccttgatca tctcggagat ggcccagaac gtgacgccct
     2761 cgccataggc aggggaccgg ccttcgtgcc aatagatgtt gtcgaccagg ccatcgatgt
     2821 atttcaggaa ctcccagacg agacgcgact tcccgatgcc ggcctccccg accagggaga
     2881 ccatccgtgc tctcgattcg gttccaaccg cgtcgaggag gtccttgagc aggcgcaact
     2941 cattggcgcg tccgacaaag ggaggctcga gggactcggc tttgccacgt cctgcccgct
     3001 cggagaggac tctggtggcc tgccatgccg cgacggggag ggtcttacct ttgaggctct
     3061 ggggtcctac cgcttcgaag gcgatcgagc ggtgcgttgc ctgctgggtg ctctcgccga
     3121 ccagcacggt tccgggctcg gcgatcgttt gaagacggga tgcggtgttc acgagatcgc
     3181 cggtcacgaa gcccatgcga tcgtcgtttg gcccgacagc agcctcaccg gtatggatcc
     3241 ccaccctgag accgagatcg ggtgcgccga cgtcggcgga gagcttggcc acaatgtcga
     3301 tgagctcgaa cccggcacga atggcgcgtt cggcatcgtc ttcggtcgcg gtctccgcac
     3361 cccacaccgc catgacggcg tcgccgatga acttctcaat gattccgccg aacagctgga
     3421 tcacacgccg gctctggtcg aaatagctcg tctggaagtc cctcacctcc tcggcgtcgc
     3481 gaccctccga gaagtcggtg aaaccggtca gatcggcgaa caggacagtg accagacggc
     3541 gttcggctgt gaccgttggg gtgagttgaa tctgcgaccc ggcgagggag gtcccgcact
     3601 gaccgcagaa attcgtcccc ggctcgttgg acgccccaca agtcgagcat gtgatggcga
     3661 gggcactgcc acagttgccg cagaatctgt tgttggccgg gttttcggtg ttacagacgc
     3721 tgcagatcac aagggcgcac catacatgcg tcggcgcgcc tgaccgttgc tggatatcat
     3781 cgtgttgcca cccatcgagg agacgtcttg gccaccaagc aggagagttt ggagttgctg
     3841 ggtggggtgc ccttcctcag cgacctcagc aagagagacc tgaacaaggt gctagccatc
     3901 gccaaaccga ccgttcacgc cgacggcacc gaagtaatga ccgagggtcg cggaggagtc
     3961 ggtttccatt tgatcaccga gggtaaggcg aaagtccagc gagcgggcag gacggtggca
     4021 acgctcgggc ccggtgagtt tttcggcgag atggcattgg tggacgacgg cccgcgctcg
     4081 gcatccatat tcgctgactc cgaactgacc actctggtga tctccaaatg ggagtttcgt
     4141 cccctcgtga agtcgcatcc cgaattggcc tggaagctga tcgagcacct ggtcgttcgg
     4201 ctccgggagg agcagaccgg ccgcgacgct ctcgtctgtt agtcgacgtc cgattcggtc
     4261 tcgaccgaga ggtctccacc ctcccaccgc gcccggacgc ggtatttcgc cgattcgctc
     4321 tcgaactcga catccacctc gggcgggccc cccttcttga tgtccgccga gaacccggcg
     4381 gcgggagatg ccgagctgag caccacctca ccgggccgat agctcacgac aaccactccg
     4441 cctccggtct ggatagtccg cgtcgaccaa gccacatcgg cgctcggtga cgtcgaggtc
     4501 gacgacgacc cagcgggggt cgacgacggc accgtgctgg tcg
//
LOCUS       DASTXO010000194         3157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_601193, whole genome shotgun sequence.
ACCESSION   DASTXO010000194 DASTXO010000000
VERSION     DASTXO010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601193"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1459)
                     /locus_tag="VFU96_04140"
     CDS             complement(<1..1459)
                     /locus_tag="VFU96_04140"
                     /inference="COORDINATES: protein motif:HMM:NF025181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin-like doman-containing protein"
                     /protein_id="HEU5112483.1"
                     /translation="MRSFLVIGLGGSGGKTIRYLKQSLGEWLNEIGWQGGMPQGWQFL
                     HIDTPSTQEAPILPGRPELLPPIEYMSLHRDGVPFGTVVDRLTRKQTNFDGWWVDPDF
                     MNVPIAHGAGQYRAIGRVLGLDQQDRIHTQLQGKITALRTADAIGELQRLRASADEEG
                     SGTYEERGEPVLMIVSSLAGGTGAGLFLDVADMLRAEGEQWLDNSFGILYAADVFQSL
                     AAGARAGVHPNSAAALAELLSGVYAGGRVAVAGEEREISRSGPAFSFLVGHANTSGVV
                     FGDQTDVYRVMGRCLSAVMTDPQVQDDFVVYTAANWQNSAGTYGDSGGPMHMLMEPPY
                     VGTLQGLGYAEVDLGVDRFKAYSERRIVRDAVDTLVNGHRLITEGLERYEGLTPDVIA
                     QELAADRLTGFLNNVGINERGQEHQVVEAIALPLRERQVITGALAEEVYRAAVENVGA
                     KGRIDEWLTAVVEEAELLAPGENERYDTKLRERARE"
     gene            complement(1456..>3157)
                     /locus_tag="VFU96_04145"
     CDS             complement(1456..>3157)
                     /locus_tag="VFU96_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112484.1"
                     /translation="RRVLRGMGGDPSLGCGPGNGSFQSVDDVSRLPFIMACASQAGGY
                     QLPGLASDGAGNLVVNEGVVDAGPVPYQLATEVRLITRGSAGNPPGLTASSLNDVTET
                     ADGDSGTNVVQARPVGAPFNVDMSGVTEVCGFVIAQAAVPAVQGVAPTLYQNEPGEFL
                     VVADGPHGRLEGEVLGRIEVAADSGQVGAPDGTGWVVTVPTLPAAERFDLTVEMTSGP
                     GLVQTATPAFTLNEQINAPAIVEQPAPISGEGVGPFLIDLQVDPKDGGELCLLNPITQ
                     MEDVQGAAITASANVAGGECVQVEPGGVQTMTMEIVLDRSGFAHDVLEFETFSTPSTS
                     PDRAEGGLLAIDLEVTPKANPVLVALIVAGLMLLMLALLWAILYGVNRLIGRIPDPRR
                     NRIRYANFVAELTPTEYGELDIAIVEAPVDSAFRIPKRTSARLDAGSLRMERVVSIVP
                     WAAPYARVGLGTDWVGAHQGPGLPGRTVLAEKAYRGRARDALGPLVTIGLGNSQLDRL
                     AEGTPQKAPGVLLFDIRAARGADASRFAAEMINDSLGLIAAEISTRRLVAEMERTGDS
                     "
BASE COUNT          510 a         1064 c         1070 g          513 t
ORIGIN      
        1 actcccgagc ccgctctcgg agcttggtgt cgtagcgctc gttctcgccg ggagcgagca
       61 gttcggcctc ctcgacgacg gcagtgagcc actcgtcgat ccgtcccttg gcaccgacgt
      121 tctccacggc ggcccggtag acctcctcgg ccagggcgcc ggtgatcacc tgacgctccc
      181 gcaatggcag ggctatcgct tcgacgactt gatgctcctg gccccgctcg ttgatcccga
      241 cgttgttgag gaatccggtg agccggtcgg cggccagctc ctgggcgatg acgtctgggg
      301 tgaggccctc gtagcgctcc agcccttcgg tgatgagccg gtggccgttg accagagtgt
      361 ccacggcatc gcggacgatc cggcgctcgg agtaggcctt gaaccgatcg accccgaggt
      421 ctacctcggc ataccccaag ccctgaagag tgccgacata cggcggctcc atcagcatgt
      481 gcatcgggcc gcctgagtcg ccataggtac cggcgctgtt ctgccagttc gcagcggtgt
      541 acaccacgaa gtcgtcctgc acctgagggt cggtcatgac cgcggagaga cagcgcccca
      601 tgacccggta gacgtcggtc tggtcgccga acacgacacc gctggtgttg gcgtggccga
      661 ccaggaagga gaaggcgggc ccgctgcggg agatctctcg ctcctcgccg gcgacggcga
      721 cccgtcctcc ggcatagacc ccgctgagca gctcggccag ggccgctgcc gagttcgggt
      781 ggacccccgc cctggcgcca gccgccaggc tctggaagac gtctgcggcg tagaggatgc
      841 cgaatgagtt gtcgagccat tgctcgcctt cggcgcgaag catgtcggcg acgtcgagga
      901 acagcccggc accggtgcca ccggcgagag aggagacgat catcaggacc ggctcccccc
      961 gctcctcgta ggtgcccgag ccctcttcgt cagccgaggc ccgaagccgc tgcagctcgc
     1021 cgatggcgtc ggcggtgcgg agcgcggtga tcttgccctg gagctgagtg tggatcctgt
     1081 cctgctgatc gagcccgagc acgcgtccga tggcccggta ctgcccggcg ccgtgggcga
     1141 tgggaacgtt catgaaatcg gggtccaccc accagccgtc gaagttggtc tgcttcctcg
     1201 tcaaccggtc gaccactgtt ccgaaaggca caccgtcgcg gtgcagcgac atgtactcga
     1261 tcggaggcag cagctcgggg cggccgggca ggatcggggc ctcctgtgtc gacggggtat
     1321 cgatgtggag gaattgccag ccctggggca tgcccccctg ccagccgatc tcgttcagcc
     1381 actcgccgag agactgcttc agatagcgga tggtcttgcc gccggagcca ccgagcccta
     1441 tcaccagaaa cgatctcatg agtccccagt cctttccatt tcagcgacca ggcgcctggt
     1501 cgagatctcg gctgcgatga gtcccagcga gtcattgatc atttcggcgg cgaacctgct
     1561 cgcatcggct ccccgggccg cccggatatc gaagaggaga acgccgggag ccttctgcgg
     1621 ggttccctcg gccaaccggt cgagctggga gttgccgagg ccgatcgtaa ccaaggggcc
     1681 gagtgcatca cgcgcccgcc cccgataggc cttctcggcg agcacggtcc tccccggcaa
     1741 cccggggcct tgatgtgccc ccacccagtc ggtgccgagg ccgacccggg catagggggc
     1801 ggcccacggg acgatcgaga ccacccgctc catgcgcagg ctgccggcgt cgaggcgagc
     1861 agaggtgcgt ttgggaatcc ggaaggcgct gtcgactggc gcctccacga tcgcgatgtc
     1921 gagctccccg tattcggtgg gcgtgagctc ggcgacgaaa ttggcgtaac ggatccggtt
     1981 ccgccgtggg tcggggatgc ggccgataag ccggttgacg ccgtagagga tcgcccagag
     2041 cagtgccagc atcagcaaca tcagcccggc gacgatgagg gcgaccagga ccgggttcgc
     2101 cttcggggtg acctcgagat cgatggcgag aaggccgcct tctgcccggt cggggctggt
     2161 gctcggcgtg gagaaggtct cgaactcgag aacgtcgtga gcaaacccgg agcgatcgag
     2221 gacgatctcc atcgtcatgg tctgtacccc gcccggctcg acctggacgc attcgccccc
     2281 ggcgacattg gcgctggcgg tgattgcggc tccctggacg tcttccatct gagtgatcgg
     2341 attgagcaga cacagctcgc caccgtcttt cggatcgacc tggaggtcga tcaggaacgg
     2401 accgacaccc tcgccgctga tcggggccgg ctgttccacg attgcaggcg cgttgatctg
     2461 ctcgttgagc gtgaacgccg gcgtcgcggt ctgtaccagg cccgggcccg aggtcatctc
     2521 cacagtcagg tcgaagcgct ctgcggccgg gagcgtgggc acggtgacca cccaaccggt
     2581 tccgtcgggg gcgccaacct gcccggagtc ggcagcaacc tcgatcctgc ccagcacctc
     2641 accttcgagc cggccatgcg gcccgtcggc gacgacgagg aactcccccg gctcgttctg
     2701 atagagcgtg ggcgccaccc cctgcacggc ggggaccgcg gcctgggcga tgacgaagcc
     2761 gcacacctcg gtcactcccg acatgtcgac gttgaacggc gccccgaccg gacgggcctg
     2821 gaccacgttg gtgccggagt cgccgtcagc tgtctcggtg acatcattca gggacgaggc
     2881 ggtgaggccg ggcgggttgc cggcacttcc tctggtgatg agccggactt cggtggccaa
     2941 ctgatacggc actggcccgg cgtcgaccac gccctcgttg acgacgagat tgccggcgcc
     3001 gtcggacgcc agaccgggca actgataccc accggcctgg gaggcgcacg ccatgatgaa
     3061 ggggagccgg ctgacatcgt cgaccgactg gaacgaaccg ttgccggggc cgcagccgag
     3121 agacggatcg ccccccatgc cgcggagcac gcggcga
//
LOCUS       DASTXO010000195         4438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_453505, whole genome shotgun sequence.
ACCESSION   DASTXO010000195 DASTXO010000000
VERSION     DASTXO010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453505"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..123
                     /locus_tag="VFU96_04150"
     CDS             <1..123
                     /locus_tag="VFU96_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU5112485.1"
                     /translation="GIEGNLCRCTGYHNIVRAVEYAGAKMRGEEPAAPDWEEAH"
     gene            123..2498
                     /locus_tag="VFU96_04155"
     CDS             123..2498
                     /locus_tag="VFU96_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEU5112486.1"
                     /translation="MATTSVMGGSVRRREDPALIQGYGEYVDDLTRTGLLHVAFVRSP
                     VARGKITTLDTEAAKKMPGVVAVYTHDDVAHLGPLVAQVPIGKLRPLLAAGEVNHVGE
                     AVAMIVAEDRYAAQDAADAIEVDYDVLPAVIDLKKAASDEVKIHDELDSNVLHTWTYH
                     GYWEALGLEDQTPQVDAAMERDDAVVVSLEMINQRLIPVAIEPRSVMAEYNKGYGHFT
                     VFSSTQVPHALGGALATTFGMKTTAVRVIAPEVGGGFGSKLNVYNDEILVAFAAKELG
                     RPVKWTETRREASGSTIHGRGWVGTATLVGTKDGEFLGYKLDALADMGAYTQNFTVAI
                     PLLGLWIGSGQYKMPISWTVACVVTNTMTTDAYRGAGRPEAIYYLERIIDAYAHEIGM
                     DPLEVRRKNFWQPDEFPATMGCGFALDSGNYEAAVYKLIAAADYEGMRRRQEEARAEG
                     RLVGVGVGSYVEVCGFGPAILADIGFSWANYGLPASFNGSGLVRVNPDATVTVVIGTG
                     PTGQGHETTWAQIVGDGLGIPVDDISVLHGDTREHPMGIGTFGSRSIAVDGAATFEAT
                     NKVKEKAAKIAAHLLEASAEDIVFEDGSAHVAGSPEKSVDWAAIAKAAYQAHTSPDDV
                     ESGLEAHTAFSPGNATWPFGTHIAMVEVDPETGNVELLEYHGVDDCGHVINPMIVDGQ
                     IHGGIAQGIGQAMFEEAIYDESGNMLTGSLLDYLLPTAADLPSFDLSRTVTPTPVNPM
                     GVKGVGEAGTIGSAHTIVNAVVDALTPMGVKHIDMPVRPKRVWQAMQEARV"
     gene            2500..3357
                     /locus_tag="VFU96_04160"
     CDS             2500..3357
                     /locus_tag="VFU96_04160"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEU5112487.1"
                     /translation="MYPRQFEYVAPSSLDEALGALGEGSKVMAGGMSLIPLMKTRLFS
                     PAVVVDIGRLEGLDAVSDAGDYISVPALVRHATTASDPLIATHAAALATAAAHTGDVQ
                     VRNRGTTCGSLAHSDVAADQPAAVIALGGKMIARSASGTREIQASEFCVDALTSALEP
                     DEILVEIQLPKMGPGEASAYDKLGRRGGHSDYAVAGAAAWVRKSNGTVEDARIGLTGV
                     GTKPSLALGVANAIVGTDGSDEAVKAAAEHALEEVTVLEDLYGSEEYKSHLAKVFVAR
                     ALKQALAAV"
     gene            3468..3728
                     /locus_tag="VFU96_04165"
     CDS             3468..3728
                     /locus_tag="VFU96_04165"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEU5112488.1"
                     /translation="MNEPCEHFVDLVPVEPTSDVCEACIELGDNWVHLRACLSCGRVG
                     CCDASKNRHARKHWEREDHPVIRSIEPGETWKYCFPDAVVSR"
     gene            3725..>4438
                     /locus_tag="VFU96_04170"
     CDS             3725..>4438
                     /locus_tag="VFU96_04170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5112489.1"
                     /translation="MTDIKPVIIAVDDEPEVVQAVQRDLRARYASDYRIVWASGGQEA
                     IDTLRSLALQDTPVALILVDQRMPGVTGIDVLRETLELHPAAKRALLTAYADTEAAIQ
                     AINDVGLDHYIMKPWDPPEDKLYPILDDLLEDWVAAYRPRFDGVRVIGQQWSRASHEL
                     KAFLARNQIPYRSMDIESDPQAWILTEEAGATMTDLPIVLLGEGPPLFNPTPAQIADR
                     VGLKTHATSEVYDVVIIGGG"
BASE COUNT          832 a         1420 c         1449 g          737 t
ORIGIN      
        1 gggatcgagg ggaatctctg tcggtgcacc ggctatcaca acatcgtccg tgcggtggag
       61 tacgccgggg ccaagatgcg gggtgaggag ccggcggctc cagattggga ggaggctcac
      121 tgatggctac taccagtgtc atgggagggt cggttcgccg gcgcgaggac ccggcactca
      181 tccagggtta cggggagtat gtggacgacc tgactcgcac gggtttgctc catgttgcct
      241 tcgtgagatc gcccgtcgcc cgagggaaga tcaccacgct cgacaccgag gcggccaaga
      301 agatgcccgg ggttgtcgcc gtgtacaccc acgacgacgt cgcccatctc ggcccactcg
      361 ttgcccaagt tccgatcgga aagctccgcc ccctgctcgc cgctggtgag gtcaaccatg
      421 tgggtgaggc ggtggcgatg atcgtcgccg aagaccgcta tgcggcacag gacgccgccg
      481 atgccatcga ggttgactac gacgtccttc cggctgtcat cgacctgaag aaggccgcgt
      541 ccgacgaggt gaagatccac gacgagctcg attccaacgt cctccatacc tggacctatc
      601 acggttactg ggaagcactt ggcctcgagg accagacgcc ccaggtcgac gccgcgatgg
      661 aacgggatga cgcagtcgtg gtcagcctgg agatgatcaa tcagcgtctc atccccgttg
      721 cgatcgagcc gcgctccgtg atggccgagt acaacaaggg atacggccac ttcaccgtgt
      781 tcagctcgac tcaggttcct catgctctgg gaggagctct ggcgaccacg ttcgggatga
      841 agaccaccgc agttcgggtc atcgcgcccg aagtcggtgg tggatttggc tccaagctca
      901 acgtctacaa cgacgagatc ttggtcgcct tcgccgccaa ggagctcggg cgcccggtga
      961 agtggaccga gactcgccgc gaggcgtccg ggtcgaccat ccacggtcgg ggctgggtcg
     1021 ggaccgccac tctggtcggg accaaggacg gtgaattcct cgggtacaag ctcgacgccc
     1081 tcgccgacat gggcgcctac acccagaact tcaccgtggc catcccgctc ttgggtctgt
     1141 ggatcggctc gggccagtac aagatgccca tctcctggac ggtggcgtgt gtcgtcacca
     1201 acacgatgac cacggacgcc tatcgcggcg ccggccggcc agaggcgatc tactacctgg
     1261 agcgcatcat cgacgcatat gcccacgaga tcggcatgga cccgctcgag gtgcggagga
     1321 agaacttctg gcagccggat gagttcccgg cgacgatggg ctgtgggttc gccctcgact
     1381 ccggcaacta cgaggcagct gtctacaaat tgatcgccgc tgccgactac gaaggaatgc
     1441 gcaggcgaca ggaagaggcc cgcgccgagg gccgactcgt cggcgtcgga gtgggctcgt
     1501 acgtcgaggt gtgcgggttc gggccggcga tcctcgccga catcggtttc tcctgggcca
     1561 actacgggct gccagcgtcg ttcaatgggt caggactggt ccgcgtcaat ccggatgcga
     1621 cagtcaccgt tgtcatcggg accggcccca ccgggcaggg ccacgagacg acctgggcgc
     1681 aaatcgttgg cgacggcctc gggattcctg tggacgacat aagcgtgctc cacggcgaca
     1741 cccgcgagca tccaatgggc atcggcacct ttggaagtcg ctcgattgcc gtcgacggag
     1801 ccgcgacgtt cgaagcgacc aacaaggtca aggagaaagc agccaagatc gctgcccatc
     1861 tcctcgaggc gtctgccgag gacatcgtct tcgaggatgg gagtgcccat gtggccggtt
     1921 caccggagaa gagcgtggat tgggcggcga tagccaaggc ggcgtatcag gcccacacca
     1981 gccccgacga cgtcgaaagt ggcctggaag cgcacaccgc cttcagcccg ggcaatgcca
     2041 cctggccctt cggcacacac atcgccatgg tcgaggtaga tccggagacg ggcaacgtag
     2101 aactgctgga ataccacggg gtcgacgact gcggtcacgt gatcaacccc atgatcgtcg
     2161 acgggcagat tcatggaggg attgcccagg gcatcgggca ggcaatgttc gaagaagcga
     2221 tctatgacga gagtggcaac atgctcaccg gatcgttgct cgactatttg ttgccgacgg
     2281 cggccgatct cccctccttc gacctcagtc gcaccgtgac gcccactccg gtcaacccga
     2341 tgggggtgaa gggcgtcggc gaggccggca ccatcggctc ggcccacacc atcgtcaacg
     2401 cggtggtgga cgctctcacc ccaatgggcg tgaaacacat cgacatgccg gtcagaccaa
     2461 agcgggtgtg gcaggcgatg caggaagcga gggtttgaca tgtacccgcg acagttcgaa
     2521 tacgtggcgc cctcctcact cgacgaggcg ctgggagcct tgggcgaggg ctcgaaggtg
     2581 atggcaggcg ggatgagcct catcccgttg atgaagacac ggctcttctc ccccgcggtc
     2641 gtcgtcgaca tcgggcgcct cgagggtctc gatgcggtga gcgacgccgg cgactacata
     2701 tccgttccgg ccttggtccg tcatgccacc acggcgtcgg acccgctcat tgccacccat
     2761 gcggccgccc tggccactgc agctgcgcac accggcgacg tccaggtgcg caatcgtggc
     2821 acgacctgcg gatcgctcgc ccactctgac gtggctgccg atcaaccggc agcggtgatc
     2881 gcgctgggtg ggaagatgat cgcccgatct gcatcgggga cgagggagat ccaggcatcg
     2941 gagttctgtg tcgatgccct gacctcggcc ctcgaacccg acgagatcct ggtcgagatc
     3001 cagctcccca agatgggccc gggcgaggca tccgcctacg acaagctggg cagacgcggt
     3061 gggcatagcg attacgcggt cgccggcgcg gccgcctggg tgaggaagag caacggaacg
     3121 gtggaggacg cccgcatcgg cctgaccggg gtgggcacca agccctccct cgccctgggt
     3181 gtggccaatg ccatcgtcgg caccgatggg tccgacgagg cagtgaaggc ggcagccgag
     3241 cacgccctcg aggaagtcac ggtgctcgag gatctctatg ggtcggagga atacaagagc
     3301 catctggcca aggtgttcgt cgcccgcgcc ctgaagcagg ccctcgccgc agtctgaccc
     3361 ggccgtggtc ctggtctgta atgcgacccg gtagggtcgc gtcgccgcgt cagaatgggt
     3421 aagacacggc cctgcgctaa tttcgggctg ggaggtagac aattgaaatg aacgagccat
     3481 gcgagcactt cgtcgacctg gttccggtgg aaccgacctc cgacgtatgc gaagcgtgta
     3541 tcgagctcgg ggataattgg gtccacctgc gtgcatgcct ctcctgcggc agggtcggat
     3601 gctgtgacgc ctccaagaac cgtcatgcac gaaagcactg ggaacgcgag gaccacccgg
     3661 tgatccgttc tatcgagccc ggtgagacct ggaagtactg cttccccgac gccgtggtgt
     3721 ctcgatgaca gatatcaagc cggtcatcat cgccgtcgac gacgaacccg aggtcgttca
     3781 ggcggtgcaa cgggacctgc gggcccggta cgcatcggac taccggatcg tgtgggccag
     3841 cggggggcag gaggcgatcg acaccctccg ctcgctggcc ctgcaagaca ccccggtggc
     3901 cctgatcctc gtcgaccagc gcatgccagg ggtcaccggt atcgatgtgc tgcgggagac
     3961 cctggagctc caccccgcag ccaagcgagc cctgctcacg gcgtacgccg acaccgaggc
     4021 cgccatccag gccatcaacg acgtgggcct cgaccactac atcatgaagc cttgggaccc
     4081 gcccgaggac aagctgtacc cgatcctcga cgacctgctc gaagactggg tcgccgccta
     4141 ccggccccga ttcgatggcg tgcgcgtcat cgggcagcaa tggtcacgtg cctcccacga
     4201 gctgaaggcc ttcctcgctc gcaaccagat cccctaccga tcgatggaca ttgagtccga
     4261 ccctcaagct tggatcctca ccgaggaagc gggggcgacc atgaccgatc tcccgatcgt
     4321 cctcctcggc gaagggccgc cgctgttcaa tcccactccc gcccagatcg cggacagggt
     4381 cggcctgaag acgcacgcca cctcggaggt ctacgacgtc gtcatcatcg gtggtggg
//
LOCUS       DASTXO010000196         8332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1485415, whole genome shotgun sequence.
ACCESSION   DASTXO010000196 DASTXO010000000
VERSION     DASTXO010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485415"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VFU96_04175"
     CDS             <1..639
                     /locus_tag="VFU96_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5112490.1"
                     /translation="DAFGSVDIVINNAGILRDKSFANMTLDEVDAVLDVHLRGAFHVS
                     HPAFQVMKEQGSGRFVHTTSASGLFGNFGQANYGAAKAGLVGLSNVLAIEGAKYNIKS
                     NAVAPTARTRMTEDILGPFVEMLDPRQVMPMVVYLCSEQNELTHEILTVGGGRYGRVF
                     IGTNTGWFAGQGVVPTVEDLSEHLEEIRDVSEPVIPGDINEELMILARLAGG"
     gene            639..1499
                     /locus_tag="VFU96_04180"
     CDS             639..1499
                     /locus_tag="VFU96_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEU5112491.1"
                     /translation="MSIDLDSAVGAELAGGTADWDEDDIILYHLGIGAGVPPTEPAEL
                     RYAYEGDLHVLPTYATIPQFPVMMSMGYAPGFDINPAMILHGEHEIEVHEPIPTSGSV
                     TQSGRVTEILDKGKGALALVEIVSVLEKTGRPLFTNRASIYIRGEGGFGGDSGAPSED
                     LTPERDPDHVVESITLPQQALLYRMASGDKNPLHADPGFAAFAGFERPILHGLCTFGI
                     ACKATVDHALSGQTDAVAAFRGRFSGVVYPGETLITSVWDEGDRLIVSAVAKERGALV
                     LTNGVMRRRT"
     gene            complement(1511..2308)
                     /locus_tag="VFU96_04185"
     CDS             complement(1511..2308)
                     /locus_tag="VFU96_04185"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEU5112492.1"
                     /translation="MRRIALSALLCLASVACGGADAPQSASTVTSTSTFVTTTTAAPS
                     PTTTVATTTTEVEVLDVASLPEGSSLVLGARETVEVYDGPHASEAIRTLEPTTILGTT
                     TVLVVVEGPSEGWARVLLPGRPNGAEGWVRTDEMVAFVVEGRVVIDLSDRTLSYLVGG
                     TEVIATPVGIGSDSHPTPTGKFFVTDSVAMADPASPWGPHALGLSARSDTVTEYNGGD
                     GIIGIHGTNNPWSIGRAMSLGCLRVANDVITRLHEVVAVGTPVDIRA"
     gene            2382..3425
                     /locus_tag="VFU96_04190"
     CDS             2382..3425
                     /locus_tag="VFU96_04190"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEU5112493.1"
                     /translation="MPRVFVSDFIPGDILEPLGALAEVEMWSQPARDLEVTPQVSLRV
                     PRQTLFESLRNCEGWLSMLSDTIDVALLDAAPRLRVISQMSVGVDNIDVGACRAHGIS
                     VGHTPDVLTETTADTAFALIGAAVRRIPEGQEIVKSGAWGPWDPWQFLSGDLHGATLG
                     IVGMGRIGQAIARRAGGFDMEVLYTTRTAKELPGATRLDLAGLLERSDIVVIAVPLAD
                     ETRGMIGRAELSSMKETAILVNIARGPIVDTDALVEALAKGVIGGAALDVTDPEPMPP
                     DHPLLQFPNCLVVPHVGSATVPARRGMASLAVANLVAYLRREPMPAWLSGSAPQPLAD
                     YSAPGSPSESSVS"
     gene            3429..4955
                     /locus_tag="VFU96_04195"
     CDS             3429..4955
                     /locus_tag="VFU96_04195"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF013461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEU5112494.1"
                     /translation="MFELPEARSIEVNPSQERLRELTLERMPRVTVTEFGNLNYQAEI
                     TARLKNSTFFVADEEIHQNRIPRAEADEWAARQDAHIADRDMLLIEGRVGPDPSSSTP
                     SRLMIEAGQANIAGMQAQLFFPPAPSGDPQFTVIYTPSLPAPGKPDDRLIIVDLEKWV
                     TRVLGSDYFGESKMGSLRMWGKLAHDWGGLALHAGLKTFAAGSTRSGDEESMLIIGLS
                     GTGKTTTTFRQQPGSLPVQDDFVALMPGGAIRTTENGCFAKTWGLDPDDEPTIYGGTT
                     RPDAWLENVSVSSDGKVDFFDTNYTANGRSTFPLANIRHRDPSDVPPARYLLMLNRSD
                     HVIPGVARLRRDQIPAFFMLGETKGTSAGGAAEAGKSLRVPGTNPFFFHDDALQGNRL
                     LELLDTVPDLEAFVLNTGRIGGEGEGSKDIRIQDSSAIVQGIVEDTIEWVTDPDFGYE
                     VATSIPGVEDGELLRPARLYDRLGRKSEYEEMVARLGKERSEYLAGYTQLDPVVAAGL
                     "
     gene            complement(4967..5590)
                     /locus_tag="VFU96_04200"
     CDS             complement(4967..5590)
                     /locus_tag="VFU96_04200"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEU5112495.1"
                     /translation="MDIILIFAGGDLPDAGIADELPSPDLVVAADSGYDTAVHLGFRV
                     DVLVGDLDSIAAAPIPGHVIVERHPEDKDQTDLDLALELAMREDPSRVVVIGGTGGRF
                     DHETSTVGLLCSTRWSLVDELDWVSSRGRAHVVHRRRVLHGDVGATVSLIPVGGTVAG
                     LTTSGLRWDLDGDTFEAGTTRGVSNVLRAPVADVRIDSGCLLVVFPS"
     gene            complement(5704..6903)
                     /locus_tag="VFU96_04205"
     CDS             complement(5704..6903)
                     /locus_tag="VFU96_04205"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU5112496.1"
                     /translation="MPTAIVLLPSTTYRAADFVRAAEGLGIDLIVASEHPPPFDMGDR
                     YLQVDCSDPDLAAAAIVRLGDDSPIDGVVAADDAGVVAAAIAGRKLGVLANEPEAAAA
                     TRDKSRQRALLGAAEVPQPRFATISPLEHGEKVAAGVGYPVVIKPFDRAAGQGVIRVD
                     RPEELAPALSRVRAIVGEGAPLLIEEFMMGEEVALEGLLRDGELTTLALFDKPDAVEG
                     PYFPETILITPSLLDESRQAECRRVAAAALAAIGIGHGPVHVEMKVDGDIVRVIEVAA
                     RSIGGLCSRSLSFGLLGTTLETLILRNAIGMEKPELRREALASGVLMIPIPRPGTFTG
                     ISGLDHVRELPHVSAIDITTTAGSHVQPPPEGDRYLGFVFARGPDRDEVEASLRRAQE
                     IITVRIE"
     gene            6954..7568
                     /locus_tag="VFU96_04210"
     CDS             6954..7568
                     /locus_tag="VFU96_04210"
                     /inference="COORDINATES: protein motif:HMM:NF013286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEU5112497.1"
                     /translation="MSEDTVLSIAPDALDMILQLRDQEPGDGEFALSIEVTGFRGPQF
                     TYELAFVALDDKKEGWVVERHADLALIFPEDDKEKLDGAALELTDQGLSMNNPNSPAP
                     PPMSAPAGDLSGPLVDQVQQVIKDQVNPAIAAHGGGAELVSVDGTIAYLRLFGGCQGC
                     GLAQVTLKQGIERILLESIPTLTQVVDVTDHASGEDPYYQSQKK"
     gene            7596..>8332
                     /locus_tag="VFU96_04215"
     CDS             7596..>8332
                     /locus_tag="VFU96_04215"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEU5112498.1"
                     /translation="MSGPVQPADASVHQGPLARIDVREGRSTTVGGVPISRVLPTKGR
                     RTVGPWCFVDLILPPDFDDPHPLEVGPHPHTGLATVTWLLEGEALHTDSLGSEQSIKP
                     GQLNLMSAGNGIAHAELGTTDGIHGIQMWLAQPEATRHGSSRFEHIPDLPVADFPAAD
                     VKVMIGEIDGNRSPAVVDSGTVGLDVTLHGRRIEIPADAGFEYAVIPIDHTLKVDDAI
                     VEPGWLALVPIGTPTLPIEVAGDGARFL"
BASE COUNT         1509 a         2664 c         2733 g         1426 t
ORIGIN      
        1 gacgcgttcg gatcggtcga catcgtgatc aacaacgccg ggatcctcag ggacaagtca
       61 ttcgcaaata tgaccctcga cgaggtggac gcagtcctcg acgtgcacct caggggcgcc
      121 ttccacgtct cacacccggc attccaggtg atgaaggagc aggggtccgg tcgattcgtc
      181 cacaccacgt cggcctcggg gctgttcggg aacttcggtc aggccaatta cggcgcggcg
      241 aaggccggcc tggttggtct gtccaacgtc ctcgccatcg agggggcgaa gtacaacatc
      301 aagtcgaatg cggtggcgcc cacagcccgc acccggatga ccgaggacat cctcggtccc
      361 ttcgtggaga tgctcgaccc gaggcaggtg atgccgatgg tggtctatct ctgctcggag
      421 cagaacgagc tcactcatga gatcctcacg gtcggtgggg gcagatacgg ccgggtcttc
      481 atcggaacca acaccggatg gttcgccggc cagggagttg tccccacggt cgaggatctt
      541 tccgagcacc tcgaggagat ccgcgacgtc agcgagccgg tcatcccggg cgacatcaac
      601 gaagagctca tgatcctcgc tcgcttggcc ggcggctgat gtcgatcgat ctagacagtg
      661 cggtcggcgc agagctggcc ggcgggaccg ccgactggga cgaggacgac atcatcctct
      721 accacctcgg tattggagcc ggagtgccgc caaccgaacc tgcagagctg cgctacgcct
      781 atgagggcga cctgcacgtg ctgcccacct acgccaccat cccccagttt cccgtgatga
      841 tgagcatggg ttatgcgccc ggattcgaca tcaatccggc gatgatcctc cacggtgagc
      901 acgagataga ggtacacgag ccgatcccca cgagcgggtc ggtgactcag agcggccgtg
      961 tcacagagat cctggacaaa ggaaagggtg cgctggcgct ggtcgagatc gtctcggttc
     1021 tggagaagac cgggcgtccg ctcttcacca atcgtgcttc gatatacatc aggggtgagg
     1081 gcggattcgg tggagacagc ggggcgccgt ctgaggatct cacgccggag cgagacccgg
     1141 atcatgtcgt cgagtcgatc accttgcccc agcaggctct gctctatcgg atggcatcgg
     1201 gggacaagaa cccgctgcac gccgacccgg gcttcgccgc ctttgccgga ttcgagcgcc
     1261 cgatcctgca cggtttgtgc acttttggga tcgcgtgcaa ggcgacggtg gaccatgccc
     1321 tttccggaca gaccgatgca gtcgccgcgt tccggggccg tttctcaggt gtcgtctacc
     1381 cgggggagac gctgatcacg tcggtatggg acgagggcga tcgactgatc gtcagcgccg
     1441 tcgccaagga gcggggggca ttggtcctga ccaacggcgt tatgaggcgt aggacctgag
     1501 tggtgagacg ctaagcccgg atatccaccg gcgtcccgac ggcgaccacc tcgtgaaggc
     1561 gggtgattac atcgttggcg acgcggaggc atccgaggga catggcacga cctatcgacc
     1621 atgggttgtt ggttccatgg atgccgatga tcccgtcgcc tccgttgtat tccgtgacgg
     1681 tgtcggatcg ggccgagaga ccgagcgcat gagggcccca cgggctggcc gggtcggcca
     1741 tggcgacgct gtccgtgacg aagaatttcc cggtcggagt ggggtggctg tcgcttccga
     1801 tgccaaccgg tgtggcgatg acctcggtgc ccccaaccag atagctcaag gtccgatcag
     1861 agaggtctat gaccaccctg ccttccacca cgaatgcgac catctcatcg gtgcggaccc
     1921 agccctccgc cccattgggc cgtccgggga gcaggacccg agcccaaccc tcactcggtc
     1981 cctccacgac cacgagcacc gttgtagtgc ccaggatggt ggtcggttcg agggtgcgga
     2041 tcgcctccga cgcgtgtggg ccgtcgtaga cctcgacggt ctctcgcgcg ccgagaacga
     2101 gggaggaccc ttcgggaagg gatgcaacgt cgagaacttc cacctccgtc gtcgtcgttg
     2161 cgaccgttgt ggttgggctc ggtgccgcgg tggtcgtggt cacgaaggtg ctggtggagg
     2221 tgacagtcga ggccgactgg ggtgcgtcag ccccaccaca ggccacggac gccaggcaaa
     2281 gcagagcgga caaggcaatg cgtcgcatct cgaggaatgg tatcggcaca ctcgaagcga
     2341 actgtgggtc tgaatgcgtg ggccattagc ctcctgaccc gatgccgagg gtgttcgtca
     2401 gcgacttcat cccgggggac atcctcgagc ccctgggggc gctcgccgag gtagagatgt
     2461 ggagccagcc cgcccgggat ctggaagtca ccccccaagt ctcactgcgg gtgcctcgcc
     2521 aaactctttt cgaaagcctg cgcaattgtg aagggtggtt gtccatgctc tcggacacca
     2581 tcgacgtcgc gctgctcgac gcggctccga gactgagagt catatcccag atgtccgtgg
     2641 gagtcgacaa catcgacgtc ggcgcctgcc gggctcatgg catctcggtc ggccataccc
     2701 ccgacgtgtt gacggagacg acggcggaca ccgccttcgc cctcatcggc gccgccgtcc
     2761 ggcgtatccc cgaaggacag gaaatcgtca agtcgggagc gtggggccct tgggatccgt
     2821 ggcaattcct cagcggggac ctgcacggcg ccacactcgg gatcgtaggg atgggcagga
     2881 tcggccaggc gatagctcgt cgtgccggtg gcttcgacat ggaggtgctc tacaccacac
     2941 ggaccgccaa ggagctcccg ggggcaaccc gtctcgatct ggccggtctg ttggagaggt
     3001 cggacatcgt ggtcatcgct gtccccctcg ccgatgagac ccgggggatg atcggtcggg
     3061 ccgagctgag ctcgatgaag gagacggcga tcctggtgaa catcgcccgc gggcctatcg
     3121 tcgacaccga cgccttggtg gaagcgctgg cgaaaggcgt gatcggaggc gctgccctgg
     3181 atgtcaccga cccggagccc atgccgccgg atcatccctt gctccaattc cccaattgcc
     3241 tggttgtgcc tcatgtgggg agcgcgacgg tcccggcgcg aagagggatg gcgagcctgg
     3301 cggtggcgaa tctggtcgcc tatcttcgcc gtgagcccat gccggcgtgg ctctcgggca
     3361 gcgccccaca acctctggct gactactctg ctccgggttc cccctctgaa tcgagtgtta
     3421 gctgaccgat gttcgagttg cccgaggcca ggtcgatcga ggtaaacccg agtcaggagc
     3481 gacttcgtga gttgaccctg gaacggatgc cacgggtaac ggtgaccgag ttcgggaacc
     3541 tcaactacca ggccgagatc accgccagac tgaagaactc gaccttcttc gtcgccgacg
     3601 aggagatcca ccaaaacagg atcccccgag ccgaggcaga cgagtgggcg gcccgccagg
     3661 acgcacacat cgccgataga gacatgttgc tgatcgaagg gagggtcggg cccgatccgt
     3721 catccagcac accgagtcgg ctgatgatcg aggccggcca ggcaaatatt gcggggatgc
     3781 aggcgcagct cttcttcccg cctgcgccga gcggggatcc ccagttcacc gtcatctata
     3841 ccccttccct gccggctccg ggaaagcccg acgaccgtct gatcatcgtc gacctcgaga
     3901 aatgggtgac gcgggtcctt ggctcggact acttcgggga atcgaagatg gggagtctcc
     3961 gaatgtgggg caagctggcc cacgattggg gtggtctcgc gttacacgcc ggactgaaga
     4021 ccttcgccgc cggctcgact cgctccggcg acgaggagtc gatgctgatc atcgggctct
     4081 cggggaccgg caagacgacg actaccttcc gacagcaacc aggatcgctg ccggtgcagg
     4141 acgacttcgt cgccctcatg ccggggggtg cgatccgaac caccgagaac gggtgcttcg
     4201 ccaagacctg ggggctcgac cccgatgacg agccgacgat ctatggcggg accacacgac
     4261 cggacgcatg gctggagaac gtctccgtca gctccgatgg caaggtcgat ttcttcgata
     4321 ccaattacac cgcaaacggg cgaagcacct tcccgctcgc caatatcagg caccgcgacc
     4381 cgagtgatgt ccctccggct cgctatctcc tcatgctcaa ccgctccgac cacgtcatcc
     4441 ccggtgtcgc caggcttcgt cgtgaccaga tcccggcgtt tttcatgctg ggggagacga
     4501 aggggacgtc ggccgggggt gcagcggagg cgggcaagag cctcagggtg cctggcacca
     4561 accccttttt cttccacgac gacgcgcttc agggcaatcg tctgctcgag ctcctggaca
     4621 cggtccccga cctggaggcg ttcgttctca acacgggtcg gatcggggga gaaggggagg
     4681 gctccaagga catcaggatc caggactcgt cggcgatcgt ccaggggatc gtcgaagaca
     4741 ccatcgaatg ggtgaccgat cccgacttcg ggtacgaagt cgccacgagc atccccggtg
     4801 tggaggacgg cgaattgctc cgaccggccc ggctctacga tcggctgggc cggaagtccg
     4861 agtacgagga aatggtggca cgcttgggca aggagcggtc cgagtacctc gccggctaca
     4921 cacagctcga cccggtggtg gcggccggtc tctgatccga tcatgtctac gaggggaaca
     4981 ccaccaggag acaccccgag tcgatcctta catcggcaac cggggcacgc agcacattgc
     5041 tcacccctct ggtggtcccc gcttcgaaag tgtccccgtc gagatcccat cgcagcccgc
     5101 tggtggtcag ccccgccacc gtcccaccga ccggaatgag gctcaccgtc gcccccacgt
     5161 cgccgtgcag gacacgtcgc cggtgcacca cgtgggcccg ccctcgggac gagacccaat
     5221 cgagctcgtc gacgagcgac caacgtgtcg agcagagcag cccaaccgtc gacgtctcat
     5281 ggtcgaagcg cccccccgtc ccgccgatga ccacgacgcg ggaggggtcc tcgcgcatcg
     5341 ccagttccag tgccaggtcg aggtcggtct gatccttgtc ctccgggtgg cgctcgacga
     5401 tcacatgccc tgggatcggc gccgccgcta tcgagtccag gtctccgacc agcacatcca
     5461 cccggaaccc gaggtggact gctgtgtcgt aaccactgtc ggcggccacg accaggtcgg
     5521 gggaggggag ctcatcggcg atcccggcat cggggaggtc gccaccggcg aatatcagga
     5581 tgatatccat aatgacaaaa tcgtcttacg cgcgttccgg cgggtaagca gtgaccgtca
     5641 agtagaggtt gagactgcga tccacggcaa acgttggtga aatcggcacc ccgatgacgg
     5701 cgatcattcg atcctcaccg tgatgatttc ctgggcgcgg cgcagcgaag cctcgacctc
     5761 gtctcgatcc ggccctcggg cgaacacgaa gccgaggtag cggtcgcctt caggaggtgg
     5821 ctggacgtga ctacccgcag tggtcgtgat gtcgatcgcc gagacgtgag ggagctcccg
     5881 gacgtgatcc aggcccgaga tgccggtgaa ggtgcctggt cgtgggatcg ggatcatgag
     5941 cactccactg gccaaagcct cgcgacgcag ctcgggcttc tccatgccga tcgcgttgcg
     6001 caggatgagt gtctccagcg tggtgccgag caagccgaag ctcaggctgc gagagcacag
     6061 gccaccgatc gatcgggcgg ccacctcgat gacccggacg atgtctccat cgaccttcat
     6121 ctcgacgtga accgggccat ggccgatccc gatggccgcc agagcggcgg ctgcgacccg
     6181 gcgacattcg gcctggcggc tctcatcgag caagctcggc gtgatgagga tcgtctcggg
     6241 gaagtagggg ccctcgaccg cgtcgggctt gtcgaagagg gcgagggtgg tcagctcccc
     6301 atcgcgcagc aggccctcta gcgctacttc ctcgcccatc atgaactctt cgatcagcag
     6361 gggagcgccc tcgccgacga tcgcacgcac acgcgacaac gccggcgcca gttcctcggg
     6421 gcgatcgacc cttatcaccc cttgtccggc agcccgatcg aagggcttga tcacgacggg
     6481 gtagccaaca ccggcggcca ccttctcgcc gtgctcaagt ggtgagatcg tggcgaatcg
     6541 tggctggggc acctcggccg caccgagcag cgcacgttgt cgcgacttgt ccctcgttgc
     6601 ggccgcggcc tctggctcgt tggccagcac accgagcttt cgccctgcta tcgcagcggc
     6661 caccactcct gcgtcgtcgg cggcgacgac gccgtcgatc ggggagtcgt cgccgaggcg
     6721 gacgatggcc gcggcggcaa gatcagggtc cgagcaatcg acctggagat agcggtcgcc
     6781 catgtcgaaa ggcggtgggt gctccgaggc gacgatgagg tcgatgccga gcccttcggc
     6841 ggcccgcacg aagtcggcgg cacggtaggt ggtgctgggg aggaggacga ttgcagtggg
     6901 catagggctg tgggcgcggg attgagtcgg gggacccgtt accatcatta cagatgtcag
     6961 aagacaccgt cctcagcatc gcgcccgacg ccctcgacat gatcctccag ttgagagatc
     7021 aggagccggg cgacggggaa ttcgccttgt ctatcgaggt gaccgggttt cgcgggcccc
     7081 aattcaccta cgagctggct ttcgtcgctc tcgacgacaa gaaggagggc tgggtcgtcg
     7141 aacgacatgc tgacctggcc ctgatcttcc ccgaagacga caaagagaag ctcgacggtg
     7201 ccgccctcga gctcaccgat caggggcttt cgatgaacaa cccgaacagc ccggccccgc
     7261 caccgatgtc ggctccggcg ggagatctgt ccggccctct cgtcgaccag gtgcaacagg
     7321 tgatcaagga ccaggtcaat ccggcaatag cggcgcatgg aggcggcgcc gagctggtct
     7381 cggtcgacgg gaccatcgcc tatctccgac tcttcggagg gtgtcagggt tgcggtctgg
     7441 cccaggtcac gttgaagcag gggatcgagc gaatcctgct cgagtccata cccaccctga
     7501 cccaggtcgt cgatgtcacc gatcatgcgt ccggtgaaga cccctactac cagagtcaga
     7561 agaagtagct cggcgccccg atccggtgag cgccgttgag cgggccggtc caacccgccg
     7621 atgcttccgt ccaccagggc cccctcgctc gcatcgacgt ccgggagggc cgttcgacca
     7681 cggttggcgg cgtgcccatc agcagggtcc tgcccaccaa gggacgaaga acggttggcc
     7741 cctggtgctt cgtcgacctg atcctgcctc ccgacttcga cgatccccat ccactcgagg
     7801 tgggccccca tccccacacc gggctggcca ccgtcacctg gctgctcgag ggcgaggcgt
     7861 tgcacaccga ttctttgggg agcgagcagt cgatcaagcc cgggcagctg aatctgatgt
     7921 cggcgggcaa tgggatcgcg cacgccgagc tggggaccac cgacgggatc cacggcatcc
     7981 agatgtggtt ggcccagccc gaggcgacga gacacggaag ctccaggttc gagcacatcc
     8041 ctgatctccc ggtggcggat ttccctgccg cggacgtcaa ggtgatgatc ggagagatcg
     8101 atggaaaccg gtcgccggcc gtggtcgact cgggcacggt gggcctggac gtgacgctac
     8161 atgggcgccg gatcgagatc cccgccgatg ccggcttcga gtatgccgtt atccccatcg
     8221 atcacacttt gaaagtggac gatgccatcg tggagccggg atggctggcc ctggtgccta
     8281 tcggcacgcc aaccctcccg atcgaggtgg ccggagatgg tgcgcgattc ct
//
LOCUS       DASTXO010000197         2360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1153798, whole genome shotgun sequence.
ACCESSION   DASTXO010000197 DASTXO010000000
VERSION     DASTXO010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153798"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="VFU96_04220"
     CDS             complement(<1..681)
                     /locus_tag="VFU96_04220"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU5112499.1"
                     /translation="MRAVRVRAPGVIEVGDVPEPDAGGSVLVRVGQVGICGTDVKILG
                     GKIPVDYPRIMGHEMVGEVVAAPPASPYPAGTRVLVDPGVACGWCHLCRAGRFNICVN
                     GGLLGRDVDGVFTEYAVVPFNRLIPVPAGISARASGVLQVLGTCVHAVKTIASFPGQT
                     AAVIGLGVAGQLITQLLRLRGMTVVGITRSEWKRDLAASTGTHFTAAPDDAPAVLAEL
                     SRGRGPDLV"
     gene            750..1436
                     /locus_tag="VFU96_04225"
     CDS             750..1436
                     /locus_tag="VFU96_04225"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU5112500.1"
                     /translation="MDPTPDLRISPPEEGFAATRQTAHEFVRSVLRRAILNGELSSGS
                     RLVQAELAAMLDVSTTPVREALRDLATEGLVQFDPHRGAIVSELSAEDVHDIYEIRRV
                     LEPLAMRQAVPNVSDALLGRLRRLHQGMLDEPHSVDWVDRNRVFHMAVYETAASPRLA
                     AIIRNLQDASVMYIGASLKYKPGLREEANHGHAEILDALEKRDVEAAVKALDEHLRTS
                     IDAFESGDEG"
     gene            complement(1444..>2360)
                     /locus_tag="VFU96_04230"
     CDS             complement(1444..>2360)
                     /locus_tag="VFU96_04230"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU5112501.1"
                     /translation="PAIYALLALVVLILGLNWPVMATGVKSIAPIWMGALRIGTATAL
                     MVGVAMARRTLVVPPRRDLPIVASMALFRLTAVTVLVFTALELVPAGRSSVVVWTTSL
                     WTVPIAAIFLGERMTGARWLGLSLGIAGVLALFEPWGFDWTEPGVAVGHAFLILAAIT
                     NASTSVHIRAHRWSIAPLDSMPWQLMAATLILVVLGFAVEGRPVIDWTPQLVWIILYQ
                     GVLASGIAFWVQIVILRNLSVVSTNLTLMAVPVVGVVSSAIILGEDVTTSLLLGLILV
                     VAGVAVNLLSDREKRAEPAVVLDPQEVP"
BASE COUNT          462 a          769 c          773 g          356 t
ORIGIN      
        1 gacgagatcg gggcctcggc cccgactcag ctcagccagg accgcgggcg catcatcggg
       61 cgcggcggtg aagtgggtcc cggtgctcgc agccagatct cgcttccact ccgaacgggt
      121 gatccccacc acggtcattc ctcgcagccg gaggagctgc gtgatgagtt gtcctgcgac
      181 accaaggccg atcactgccg cggtttgccc aggaaaggag gcgatcgtct ttaccgcatg
      241 gacacaggtg cccaacacct ggaggacacc tgaagccctt gcggagatac cggccggcac
      301 cggtatcaaa cggttgaaag ggacaaccgc gtactcggtg aaaacgccat cgacatcgcg
      361 gccaagcagg cccccgttga cacagatgtt gaaacggccg gcccggcaca ggtggcacca
      421 gccgcacgca acgccggggt cgaccaagac cctggtccct gcgggatatg gcgacgccgg
      481 cggggccgcg actacctcgc ccaccatctc gtggcccatg atccggggat agtcgaccgg
      541 gatcttgccg cccaggatct tcacgtcggt gccgcagatg ccgacctggc ctacccggac
      601 gaggactgag cccccggcat ccggctcggg cacgtcgccg acttcgatca ctccaggggc
      661 ccggacacgt accgctctca catcactcct cgcaaggtgc gtggatggta gaagccgttc
      721 cggttcgccg actttgtagg ctgactccca tggatccgac acccgacctt cgaatttccc
      781 cgcccgagga agggttcgcc gccacccggc agactgcgca cgaattcgtg cggagcgtcc
      841 tgcgcagggc catcctcaat ggcgagctga gcagcggcag tcgattggtt caggcggagt
      901 tggccgcaat gctcgacgtg agcaccacgc cggtgcgtga ggcactgcgc gatctggcca
      961 cggagggact ggtccagttc gatccccatc ggggggcaat cgtgtcggag ctcagcgcag
     1021 aggatgtgca cgacatctac gagattcgcc gggtgctgga gccgctggcc atgagacagg
     1081 cggtgcccaa cgtgagcgat gcccttctcg gccgtctgcg tcggctccac caggggatgc
     1141 tcgacgagcc gcactcggtg gattgggtgg atcgaaatcg ggtcttccac atggcggtct
     1201 acgagacggc tgcctctccg cgattggccg ccatcatccg caatctccag gacgcttcgg
     1261 tcatgtatat cggggcttcc ctgaagtaca aacccggtct gcgcgaggag gccaaccacg
     1321 gtcacgccga gatcctcgat gccttggaga agcgcgatgt ggaagcggca gtgaaggcgt
     1381 tggacgagca tctccgcacg tcgatcgacg ccttcgagtc cggcgacgaa ggctgaggcc
     1441 ggatcacggc acctcctggg ggtcgaggac taccgccggc tctgctctct tctctcgatc
     1501 agacagcagg ttcacggcaa caccggcgac caccaggatc agcccgagga gcagtgaagt
     1561 cgtgacatcc tcgccgagga tgatggccga ggacacgacg cccaccaccg gcaccgccat
     1621 cagggtgaga ttggtggaaa cgacggagag atttcgcaga atcacgatct gcacccaaaa
     1681 ggcgatcccg ctggccagca cgccctgata caggatgatc cagacgagtt gcggcgtcca
     1741 gtcgatcacg ggacggcctt cgaccgcgaa gccgaggacg acgaggatca gcgtcgccgc
     1801 catgagctgc cacggcatcg agtcgagggg agcgatgctc cagcgatggg cgcggatatg
     1861 gaccgacgtc gaggcgttgg tgatcgcagc gaggatgaga aaggcgtgac ctacggccac
     1921 gccgggctcc gtccagtcga aaccccacgg ctcgaacagg gcgaggactc cggcgatgcc
     1981 gagtgacagg ccgagccatc gcgctcccgt catcctctcg ccgaggaaga tcgccgcgat
     2041 cggcacggtc cacaggctcg tcgtccatac caccaccgaa gagcggccgg cagggacgag
     2101 ctcgagtgcc gtgaagacaa ggacggtgac cgcggtgagg cggaacaacg ccatcgaggc
     2161 gacgatgggg aggtcgcggc gcggtggcac caccaacgtt ctcctcgcca tggccactcc
     2221 gaccatcagc gccgttgccg tgccgatgcg aagagccccc atccagatgg gagcgatcga
     2281 cttcactccg gtcgccatca ccggccagtt gaggccgaga atgaggacga ccagcgccag
     2341 gagggcgtag atcgccggtc
//
LOCUS       DASTXO010000198         2046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_822256, whole genome shotgun sequence.
ACCESSION   DASTXO010000198 DASTXO010000000
VERSION     DASTXO010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822256"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..1045
                     /locus_tag="VFU96_04235"
     CDS             89..1045
                     /locus_tag="VFU96_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU5112502.1"
                     /translation="MTATTITRARRLLRRPDTGGTGGRDNNALIGQSRATPYIFSLPA
                     ILLVAAILGFPVIYGTWQSFFRAPSLGAPVEFVGFENYVDMFSGPEFWHALNRTAVFV
                     FGSLILGTVLGLLFSFALFRVVGKLRFLRAVTVAPYIISNVAAAVMFRLLFNSQFGAV
                     NQVIEFFGLDGLGWLSDPGLAMFVVIVTQVWTDLPLTILVLLGGLMTIDKSYLDAALV
                     DGATGWTRARSLTIPLLTPQLVISTVWLSYSTLTGLGVVLAMTGGGPLLATQTLAMEM
                     YTVAFRALEFNQALAIATFILILNAVLTLAYVGVARRYEYEQ"
     gene            1042..1977
                     /locus_tag="VFU96_04240"
     CDS             1042..1977
                     /locus_tag="VFU96_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU5112503.1"
                     /translation="MTGAQRPGGVPLSTETAAAAGAVTGPSRNLGRPAGGGFRRAMRQ
                     ILLAIPLIALVAWTVAPFLVTTSVSFKEKGDVFSEPGLIPRDPTLDAYREVLSSESFT
                     SSFVNSVVVGVGTTLLTLAIGVPAAYAFARFRFRGRHLLLLFTLLPRLVPSLGIMVPI
                     YRLAVATGQLDKRTTLIIVYTGMLLPLAVWLMVGFFQQIPRDIEEAANVDGASLWTRL
                     RYIVVPLAAPAMITIGVLAFREAWNEFTLVLVLTSTPGKRTLPFELYLMQGIEGLANF
                     PAEAAFTLLTVLPFVLVYTRVERYVVAGLVSGSGK"
BASE COUNT          322 a          699 c          604 g          421 t
ORIGIN      
        1 gtcatcgtgc catgaccgac acacccacgt tcacgcccgt gaacgtgggt gtgccattct
       61 cggggtgctg actcgaagaa acggctgaat gaccgcgacc acgatcacaa gagcgaggcg
      121 tttgctccgg cgacccgaca cgggtggcac cggagggcgt gacaacaacg ccctgatcgg
      181 acagagcaga gccaccccct acatcttcag cctccccgcc atcctcctgg tggcagcgat
      241 cctcggattc cccgtcatct atggaacctg gcagagcttc ttcagggcgc cgagcctcgg
      301 ggcaccggtc gagttcgtcg gtttcgagaa ctacgtcgac atgttctccg ggcccgagtt
      361 ctggcacgcg ctcaatcgga ctgctgtttt cgtcttcggt tccctcatcc tcggaacagt
      421 cctcggcctc ctcttctcct tcgccctgtt ccgggtggtc gggaagctcc gatttctccg
      481 ggcggtgacc gtcgctccct acatcatctc caatgtcgcc gccgccgtga tgttccggct
      541 cttgttcaac agtcagttcg gggcggtgaa ccaggtcatc gagttcttcg gcctcgatgg
      601 gctgggatgg ctgagcgacc cggggctggc gatgttcgta gtcatagtga cccaggtctg
      661 gaccgatctc ccgctgacca tcctcgtgct gctcggcggt ctcatgacca tcgacaagtc
      721 ctacctcgac gctgcattgg tggacggggc aacggggtgg acgagggccc gctccctcac
      781 gatcccgctg ctcacaccgc aactcgtgat cagcaccgtc tggctcagct actcgaccct
      841 gaccggtctc ggcgtcgtgt tggccatgac aggtggtggc ccgctcctgg ccacacagac
      901 ccttgcaatg gagatgtaca cggtggcctt ccgcgccctc gagttcaacc aggcgctggc
      961 cattgccacc ttcatcctca tactgaacgc tgtcctcacc ctcgcctacg taggcgtggc
     1021 ccgccgctac gagtacgagc aatgacgggc gcacagagac ccggaggtgt gccgctgtcg
     1081 acagagacgg ccgcggccgc gggggcggtg accggcccat cacgaaatct ggggcgccca
     1141 gccggaggcg ggttccggcg tgccatgcgt cagatcctcc tcgccatccc actcatcgct
     1201 ctcgtggcct ggaccgtggc tccgttcttg gtcaccacgt ccgtttcctt caaggagaag
     1261 ggcgacgtct tctccgagcc cggactgatc ccgagagatc ccacactgga cgcctaccgc
     1321 gaggtgcttt ccagcgagag cttcacctca tccttcgtca acagcgtcgt cgtgggcgtc
     1381 ggcacgactc tgctcacact ggcgatcggg gtgcccgccg cctatgcctt cgcccgcttc
     1441 cggttcagag gccgccacct cctccttctc ttcaccctcc tcccccgcct ggtgcccagc
     1501 ctcgggatca tggttccgat ctaccgactg gcagtcgcga ccggtcagct cgacaagcga
     1561 acgacgctga tcatcgtcta caccgggatg ctcctgccgc tggcggtgtg gctgatggtc
     1621 ggcttcttcc aacagatccc gcgcgacatc gaggaggcgg ccaacgtgga cggagcctcc
     1681 ctgtggacgc ggcttcgcta catagtggtt cctttggccg cgccggcgat gatcaccatt
     1741 ggtgtgctgg ccttccgtga ggcctggaac gagttcaccc tggtgttggt gctcaccagc
     1801 acaccgggga aacgcaccct ccccttcgag ctctatctga tgcaggggat cgaaggcttg
     1861 gcgaacttcc ccgccgaggc ggccttcacg ctgctgacgg tgctcccctt cgtcctcgtg
     1921 tacacgcgtg tggagagata tgtcgtggcc ggactggtgt ccggatctgg caagtagtga
     1981 ccaccgtcct ggtatctgag tacctgcccg atgcatacgt ggagatgctc cgagctcgtc
     2041 atgagg
//
LOCUS       DASTXO010000199         2132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_196095, whole genome shotgun sequence.
ACCESSION   DASTXO010000199 DASTXO010000000
VERSION     DASTXO010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196095"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..>2132)
                     /locus_tag="VFU96_04245"
     CDS             complement(97..>2132)
                     /locus_tag="VFU96_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020501339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5112504.1"
                     /translation="ATGLGALQTMANTEILDIETDRGRVQAVVTDKGRVEAEYVVIAC
                     GVWSPRIARMAGASIPLTPAVHQMIDVGPIPILQESGKELAFPIVRDMDTFMYERQTS
                     GSMEVGSYAHRPIFHSPDEIPSIAEARLSPTELPFTDDDFDAQLEDALELMPDILSGA
                     EIKYAINGLLSLTPDAMPLIGETVEVRNLWSAAAIWIKEGPGTGRAVAEWMTNGYPEL
                     DPHASDVARFYPYARTEHHVRARCAEHFNKTYGIVHPREQWASERDKRLSPVHSRTSE
                     LGAVYFEAGGWERPHWYESNASLVDRYGIEDRPHEWDRRWWSPIQNAEHLAMRETVGL
                     VDLAPFVIFDFTGPGVVDYIQRMTVNNADVAVGRSVYTPLLTGSGGFKADLTVLRLGE
                     EHFRVITGAFDGPRDKHWFGIHLPDDGSVHLSDSTSAYTTIGVWGPEARNLVSSITDH
                     DLSNEAFPYGTTQAVDFGSVNARLFRISYVGDLGWEVYVPSEAGGPMWDQLWEAGQDL
                     GVVAVGAGVYGTTGRLEKGYRLMGSELESEYNPVEAGLARPRVKEHDFIGKEAYLTAR
                     DEEPAAILCTLTVEDHVSPRDGIKRYPTGREPITTPEGGRIIDSKARPSYVTTAGAGP
                     SVGKFLLMSYLPPEHAVVGNDLAVMYMNELYPVKVAVAGSTPLFDPDDARMKG"
BASE COUNT          361 a          692 c          700 g          379 t
ORIGIN      
        1 tgaaggggag atgtcgggcg tcgatcgcct ggtgatcatc ggtgaggacg attcgtgcac
       61 caggggcggg gacccgcttg atgcagacga ggattctcac cccttcatcc gcgcatcatc
      121 gggatcgaac aggggggtcg acccggcgac cgcgaccttc acgggataca gctcgttcat
      181 gtacatcacc gccagatcgt ttccaaccac cgcgtgctcc gggggcagat aggacatcag
      241 gaggaacttg cccaccgagg ggcccgcgcc ggcggtcgtg acgtacgagg gccgcgcctt
      301 cgaatcgatg atccgaccac cctcgggggt ggtgatcggc tcccgcccgg tgggatagcg
      361 tttgatcccg tcacggggag acacgtggtc ctccacggtg agcgtgcaca ggatcgccgc
      421 gggctcctcg tcacgggcgg tcagataggc ctctttcccg atgaagtcat gctctttcac
      481 ccgtggcctg gccaaccccg cctctaccgg gttgtactcg ctctccagct ccgaccccat
      541 cagtcgatag cctttctcca gacgccccgt ggtcccgtac accccggcgc caacggctac
      601 gacaccgagg tcctgacccg cttcccacag ctggtcccac atcggccctc ccgcttcgga
      661 gggcacataa acctcccagc ccaaatcgcc tacgtaggag atgcggaaga gccgggcgtt
      721 gaccgagcca aagtcgactg cctgggtcgt gccgtagggg aacgcctcat tggagaggtc
      781 gtggtcggtg atggatgaca ccaggtttcg cgcctcgggt ccccacaccc cgatcgtggt
      841 gtaggccgag gtggaatcac tcaggtggac cgacccatcg tccgggagat ggattccgaa
      901 ccaatgcttg tctctcggcc cgtcgaacgc gcccgtgatc accctgaaat gctcctcgcc
      961 gagtcggagc acggtcagat ccgccttgaa cccaccggat ccggtgagca agggtgtgta
     1021 gaccgagcga cccacggcga catcggcgtt gttgaccgtc atccgctgga tgtagtcgac
     1081 gaccccgggc ccggtgaaat cgaagatgac gaagggggcg aggtcgacca gcccgaccgt
     1141 ctcgcgcatg gcgagatgct cggcgttctg gatcggcgac caccagcgcc ggtcccattc
     1201 gtggggtcgg tcctcgatcc cgtatcgatc gacgagcgaa gcgttcgact cgtaccagtg
     1261 ggggcgctcc caacctccgg cctcgaaata gaccgcgccc agctcgctgg tgcgggagtg
     1321 cactggggag agcctcttgt ccctctcgga agcccactgc tcccggggat ggacgatccc
     1381 gtacgtcttg ttgaaatgct cggcgcagcg ggcgcggacg tgatgctccg tccgggcgta
     1441 ggggtagaag cgggcgacat cggaggcgtg agggtcgagc tcgggatagc cgttggtcat
     1501 ccactccgcc accgcccgcc ccgtcccggg cccttccttg atccagatgg ctgccgccga
     1561 ccagagattc cgcacctcga cggtctcgcc gattaggggc atcgcgtcgg gggtgaggga
     1621 gagcaggcca ttgatggcgt acttgatctc tgctccggag aggatgtcgg gcatgagctc
     1681 gagggcatct tccagttggg catcgaagtc gtcgtccgtg aaggggagct cggtcgggga
     1741 gagccgggcc tcggcgatcg aagggatctc gtcgggggag tggaatatcg ggcggtgggc
     1801 gtaggagccg acctccatcg accccgaagt ctgccgctcg tacatgaagg tatccatgtc
     1861 ccggacgatg gggaaggcga gctcctttcc ggactcctga aggatcggaa tcggcccgac
     1921 gtcgatcatc tgatgcaccg ccggggtcag ggggatcgac gccccggcca tccgggcgat
     1981 cctggggctc cacaccccgc aggcgatgac gacgtactcg gcctcgaccc ggcctttgtc
     2041 ggtgaccacc gcctgcaccc gccctcgatc ggtctcgatg tcgaggatct cggtgttggc
     2101 catggtttgc aacgccccca gtcccgtcgc ct
//
LOCUS       DASTXO010000200         2592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_528107, whole genome shotgun sequence.
ACCESSION   DASTXO010000200 DASTXO010000000
VERSION     DASTXO010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528107"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /gene="arc"
                     /locus_tag="VFU96_04250"
     CDS             <1..1157
                     /gene="arc"
                     /locus_tag="VFU96_04250"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HEU5112505.1"
                     /translation="RIDVKTGLILEKMERPEVEEIVLEEVPDITYEDVGGLADQIEQI
                     RDTVELPYMHKDLFREYELDPPKGILLYGPPGCGKTLIAKAVANSLAKKVSEQTGSPD
                     VRSYFLNIKGPELLNKWVGETERQIRLIFQRAKEKSDEGVPVIVFFDEMDSLFRTRGT
                     GISSDVESTIVPQLLSELDGVESLKNVVVIGASNREDLIDPAILRPGRLDVKIRINRP
                     DTSAAREIFRIYISNETPLDPVALEQEGGDKANVISAYIDATVDAMYAESDENRFLEV
                     TYASGDREVLYFKDFASGAMIENIVRRAKKDAIKRQIAGGEPGVRLSDLTDAIKQEFR
                     EQEDLPNTTNPDDWARVSGKKGERIVHVRTLVDGDDESRLIEKVSAGQYL"
     gene            1157..>2592
                     /gene="dop"
                     /locus_tag="VFU96_04255"
     CDS             1157..>2592
                     /gene="dop"
                     /locus_tag="VFU96_04255"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HEU5112506.1"
                     /translation="MAIHKVLGTETEYGIVVRGDIGFNPAVASSLVVNSYPGDRVKVQ
                     WSYEEESPGRDARGFGHDTFGVVDPEGAVVNSVLANGARLYVDHAHPEYSAPECYDPL
                     EAALYDKVGEVVMRSAARAATEVAGKKVSLYKNNSDGKGNSYGAHENYLLSRETPFGD
                     VIRFATTFLVTRQIFTGAGKVGSENDRPGVGFQLTQRADFFEEEVGLETTLKRPIINT
                     RDEPHGDPSKYRRLHVIIGDANLSEPQNFLKLGATALMLSALEDGALPDPLDLQDPVE
                     SCWRVSHDIDLGKPLELDGGGTATALELQGRYLEWLTKYVEKELDDPVWEQVIHEWER
                     TLDLLATDPGRLADTLDWVAKQRLFEGYIDRDGLTWEDPKLRALDLQYHDVDPEKGLY
                     HRLVGRGAMRRLFDDDEIEAAAVNPPSRTRAYFRGRCVSRFSGSLVAANWDSLVFDTG
                     ESHLKRVPMMEPLRGGADLIGAMIDEAE"
BASE COUNT          534 a          797 c          823 g          438 t
ORIGIN      
        1 tccgcatcga tgtcaagacg ggtctcatcc tggagaagat ggagcgaccc gaggttgaag
       61 agatcgtcct ggaagaggtg ccggacatca cctatgagga cgtcggaggt ttggccgacc
      121 agatcgagca gatccgtgac accgtggagc tgccgtacat gcacaaggat ctgttccggg
      181 agtacgagct cgaccccccc aagggcatcc tgctctatgg ccctcccggt tgcgggaaga
      241 cgctcatcgc caaggccgtg gccaacagcc tcgccaagaa agtgtcagag caaaccggca
      301 gccccgacgt tcgctcctat ttcttgaaca tcaagggccc cgagctgctc aacaagtggg
      361 ttggtgagac ggagcgtcag atccggctga tattccagag agccaaggag aagtcggatg
      421 aaggggtgcc tgtcatcgtc ttcttcgatg aaatggactc tctgttccgc accaggggca
      481 ccggcatcag ctcagacgtc gagtcgacga tcgtgcccca gttgctctct gagctcgacg
      541 gagtggagtc cctgaagaac gtcgtggtca tcggcgcctc caaccgggag gacttgatcg
      601 atcccgccat cctcagaccg ggccggctcg atgtgaagat caggatcaat cggcccgata
      661 cctcggcagc acgggagatc ttccggatat atatcagcaa cgagacaccc ctcgatcccg
      721 tcgccctgga acaagaagga ggggacaagg cgaacgtgat ctcggcctat atcgacgcca
      781 cggtcgacgc catgtatgcc gagtcggacg agaaccgatt cctcgaggtc acctacgcct
      841 cgggcgaccg cgaggtcctg tacttcaagg atttcgcctc cggtgcgatg atcgagaaca
      901 tcgtgaggcg cgccaagaag gacgcgatca agagacaaat cgccggcggg gagcccggtg
      961 tccggctgtc ggatctcacc gacgccatca aacaggaatt ccgggagcag gaagacctgc
     1021 ccaacacgac caaccccgat gactgggcca gggtgtccgg caagaagggg gagcggatcg
     1081 tccatgtccg gacgctggtc gacggcgatg acgagagtcg attgatcgag aaggtctcag
     1141 cgggtcaata tctctgatgg cgatccacaa ggtgctcggc accgaaaccg agtacggcat
     1201 cgtcgtcagg ggggacatcg ggttcaatcc ggcagtcgcc tcctcgctcg tggtcaacag
     1261 ctatcccggc gatcgggtca aggtccaatg gtcgtacgag gaagagtcgc caggccgtga
     1321 tgcaaggggt ttcggccacg acaccttcgg cgtggtcgac ccggagggag cagtggtcaa
     1381 ctcggtgctc gccaacgggg cccggctcta cgtggaccac gctcatcccg agtactcggc
     1441 gcctgagtgc tacgacccgt tggaggcggc cctctacgac aaggttggag aggtcgtcat
     1501 gcgctccgcg gcgagagcgg cgaccgaggt cgccggaaag aaggtctccc tctacaagaa
     1561 caactcggat gggaagggga actcctacgg cgctcacgag aactatctgc tctcccgaga
     1621 gaccccgttc ggagatgtca tccgcttcgc caccacgttc ctggtgaccc ggcagatctt
     1681 caccggcgcc gggaaggtcg ggtccgagaa cgaccgcccc ggggtcggct tccagttgac
     1741 ccagcgcgcc gacttcttcg aagaagaggt ggggctggag accacgctga agcgcccgat
     1801 catcaacacg cgtgacgagc ctcacggtga cccgtcgaag taccggcggc tccacgtcat
     1861 catcggcgac gccaacctct ccgagcccca gaacttcctc aagctcgggg caacggcgct
     1921 catgctctcc gcattggagg acggggcgct tccggacccc ctcgacctcc aggaccctgt
     1981 ggaatcgtgc tggcgggtca gccatgacat cgatctgggc aagccgttgg agctcgatgg
     2041 cggggggact gcgacagcac tggagctgca gggccgctat ctggaatggc tgaccaagta
     2101 tgtggagaag gagttggacg acccggtctg ggagcaggtc atccacgagt gggagcgaac
     2161 gctcgatctg ctggccacag atcccggccg gctggcggac acccttgact gggttgccaa
     2221 gcaacggctg ttcgagggat acatcgacag ggacggcctc acctgggagg accccaagct
     2281 gcgtgccctc gacctccagt accacgacgt cgaccccgaa aagggcttgt atcaccgcct
     2341 cgtcggacgc ggagcgatgc gtcgtctgtt cgacgacgac gagatcgaag cggccgccgt
     2401 caacccaccg agccgtaccc gggcctactt ccggggccgc tgcgtttccc ggttctccgg
     2461 ctcgctggtg gccgccaact gggattccct cgtcttcgac accggcgaat cgcatctcaa
     2521 gcgggttcct atgatggaac cacttcgagg tggcgccgat ttgatcgggg ccatgatcga
     2581 cgaagccgaa ga
//
LOCUS       DASTXO010000201         2906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_638561, whole genome shotgun sequence.
ACCESSION   DASTXO010000201 DASTXO010000000
VERSION     DASTXO010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638561"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VFU96_04260"
     CDS             <1..659
                     /locus_tag="VFU96_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112507.1"
                     /translation="RIYASFNRQIGTYTNVGVSSSFSWIMNNNDNGNDSQIWNISLTG
                     SHGVPTGFSVSGRIGYSLFDSERGDPSHLFSFSVNGSYRFARGVISAGVFQDFRQTAD
                     EGEDFGIVATRAVFASASYSFTPFITGTVHARYSRNEPVQGGGSTTPPTTYLTAGANI
                     SWLITNWLSASLAYLYIDRNQDRSTNIGSNDIDTSRIPAQFLQNSTENRATLTLSASF
                     "
     gene            896..2266
                     /locus_tag="VFU96_04265"
     CDS             896..2266
                     /locus_tag="VFU96_04265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family XrtA/PEP-CTERM system
                     glycosyltransferase"
                     /protein_id="HEU5112508.1"
                     /translation="MLRIFRHYVPQLPLILFAGDLAVLALTFHIPRLTGTWIAEGPVI
                     PRFVLVALVVGFVLNLGGLYDVRLAMGRRELLARLLTCQAVAGLLVAAIGFAIPNLRL
                     GRAAFLQIGAAATLGLIAWRATWIGPFSHRRIRIRVLVLGTGAIGKVIAGLVETGSRP
                     FAIVGFLDDNPNAPETVPAPYALLGKIQDLPALVEETRPDLIVVAQMNRRGNFPAKAL
                     IDCRMRGIQVEDWPTFYEKETGKILVTDLRPSWLIFSEGFVKTPRTEIVKRLVDVGLS
                     LLGLVLALPLMGLVALAIKLESKGPALFRQPRLGQNGRVFILNKFRSMREDAEKETGP
                     VWAQQEDPRVTRVGAFLRKTRLDELPQLFNVLVGDMSFIGPRPERPEFVYELQKQIPF
                     YMERLSVKPGITGWAQVKYRYGASVEDALEKLQYDLYYIKNLSLFLDLLILINTIQVV
                     LFARGR"
     gene            2312..>2906
                     /locus_tag="VFU96_04270"
     CDS             2312..>2906
                     /locus_tag="VFU96_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112509.1"
                     /translation="MNNVQILKQIALSVFSRRKKWVLLVSLLGVALFMPVAYVMSKEP
                     PRHRTVATILMETRPDRAPLFQEFSPFRPLDVQLAILRSRSLSEAVVESLPRNSVDDL
                     VQNPYSVDYNLEFQNWIRRLRGEEVVVESPQRRALNELRASRVRFRPLGESGIVEITA
                     EASQPRVAMDIANTYIEVLVSRTRSFNVEDSKTTREFL"
BASE COUNT          488 a         1030 c          857 g          531 t
ORIGIN      
        1 agcgcatcta cgcgtcgttc aatcggcaga tcggcaccta cacaaacgtg ggagtgtcgt
       61 cgtcgttctc ctggatcatg aacaacaacg acaacggcaa cgactcgcag atctggaaca
      121 tctcgctcac cggctcccac ggtgtcccga ccggattctc cgtctccggc cgcatcggct
      181 acagcctctt cgattcggag cgcggtgacc cctcgcatct cttctcgttc agcgtgaacg
      241 gtagctatcg cttcgcccgc ggcgtcatct cggcaggcgt cttccaggac tttcgccaga
      301 ccgcggacga gggtgaggac ttcggcatcg tcgcgacccg cgcggtcttc gcctccgcca
      361 gctactcgtt cacgcccttc atcaccggaa cggtccacgc gagatactcc cggaacgagc
      421 cggttcaagg gggaggctcg accacgcccc cgaccaccta tctcacggcg ggggccaata
      481 tcagctggct gatcacgaac tggctctcgg cgtctctcgc ctacctctac atcgatcgga
      541 accaggaccg ctcgaccaac atcgggtcca atgacatcga cacaagccgg atccccgccc
      601 agttcctcca gaactccacc gaaaaccgcg caactctgac gctgagcgcc agcttctagc
      661 attcccccac gcaattcatg cgtggctgcg ctcgttcctc aaacccttga ttacaggaca
      721 gattttcact ccctgtcgtt ttgccatgca gaatcagcag agcgagaaaa tcggtaccac
      781 ttgctggtag gtcggtaccg ccttcgtgag ccttagtggc ccaataaatc aaatacttag
      841 ctcacggcat ggcgggggct cttggcaccc cgattgctcc ctcttgcccc cggtcatgct
      901 caggatcttc cggcactacg tcccccagct tccactgatc ctcttcgcgg gcgacctcgc
      961 cgtactcgcc cttaccttcc acattccacg cctcaccggc acctggatcg cggaggggcc
     1021 ggtcatcccc cgcttcgtgc tggttgcgct ggtggtggga tttgtcctca acctgggcgg
     1081 gctctacgac gtccgcctgg ccatgggacg ccgcgagctg ctggcccgcc tgctcacctg
     1141 tcaggccgtc gccggcctgc tggtcgcggc gatcgggttc gcgatcccca acctccggct
     1201 gggccgcgca gccttcctcc agatcggcgc ggccgccacc ctcgggctca tcgcctggcg
     1261 ggcgacctgg atcgggccgt tctcgcaccg gcgcatccgt atccgggtgc tcgtcctggg
     1321 gaccggcgcc atcggcaagg tgatcgccgg cctcgtcgag accgggtccc gcccgttcgc
     1381 gatcgtgggg ttcctcgacg acaacccgaa cgcccccgag acggtccccg ctccctacgc
     1441 gcttctcggc aagatccagg accttcccgc cctggtggaa gagacgcgtc ccgacctcat
     1501 cgtggtggcc cagatgaacc gccgggggaa cttcccggcc aaggctctca tcgactgccg
     1561 gatgcggggt atacaggtcg aggactggcc gaccttctac gagaaggaga cgggcaagat
     1621 cctcgtgacg gacctccggc cgagctggct gatcttctcc gagggcttcg tcaagacccc
     1681 gcgcaccgag atcgtgaagc gcctggtgga cgtagggctc tcgctcctcg ggctcgtgct
     1741 cgcgctgccc ctcatggggc tggtggctct ggcgatcaag ctggagtcga aggggcccgc
     1801 cctgttccgg cagccccgcc tcgggcagaa cggccgggtg ttcatcctga acaagttccg
     1861 gtccatgcgg gaggacgccg agaaggagac ggggccggtg tgggcccagc aagaggaccc
     1921 tcgcgtcacg cgggtgggcg ccttcctccg caagacccgg ctcgacgagc tgccgcagct
     1981 cttcaacgtg ctggtgggcg acatgagctt catcggcccg aggcccgagc ggcccgagtt
     2041 cgtatacgag ctgcagaagc agattccctt ctacatggag cgcctctccg tgaagcccgg
     2101 tatcacggga tgggcgcagg tcaaataccg ctacggcgcg tcggtggagg acgcgctgga
     2161 gaagctccag tacgacctct actacatcaa gaatctgtca ctgttcctgg acctgctcat
     2221 cctgatcaac accatccagg tagtgctgtt tgcgcggggg cgctgaggcc atgacggccc
     2281 gcccgaaaat cgcacgagga gatcgacgcc gatgaacaat gtgcagatcc tcaagcagat
     2341 cgctctttcc gtcttctccc ggcggaagaa atgggtgctg ctcgtctccc tcctgggcgt
     2401 ggcgctcttc atgcccgtcg cctacgtgat gtccaaggaa ccgccccgtc accggacggt
     2461 ggcgaccatc ctcatggaga cgcggccgga ccgggccccg ctcttccagg agttctcgcc
     2521 gttccggccc ctggacgttc agctcgccat cctccgcagc cgcagcctgt ccgaggcggt
     2581 ggtggagtcg ctgccccgga actccgtgga cgacctcgtg cagaacccct acagcgtcga
     2641 ctacaacctg gaattccaga actggatccg acggctccgc ggcgaagaag tggtcgtcga
     2701 gagcccgcag cggcgcgccc tgaacgagct ccgcgcttcc cgcgtgcgct tccggccgct
     2761 cggggagtcc ggcatcgtcg agatcaccgc ggaggcctcg cagccccgcg tggccatgga
     2821 catcgccaac acctacatcg aggtcctcgt ctcccgcacg cggtccttca acgtggaaga
     2881 ctccaagacc acccgcgagt tcctgg
//
LOCUS       DASTXO010000202         2871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_969838, whole genome shotgun sequence.
ACCESSION   DASTXO010000202 DASTXO010000000
VERSION     DASTXO010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969838"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VFU96_04275"
     CDS             complement(<1..297)
                     /locus_tag="VFU96_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5112510.1"
                     /translation="MPHGQILSNGGGYEVTDPVRWGVIGCADIAVKKVIPAMHGSELS
                     HVTAIASRSSDRARATADTLGIPRSYGSYEELLADPEIEAVYIPLPNHLHPEWAI"
     gene            327..1979
                     /locus_tag="VFU96_04280"
     CDS             327..1979
                     /locus_tag="VFU96_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008194522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEU5112511.1"
                     /translation="MTGRAWAWLVAGVAAGALVAGALAMGAVAVFGSDLPDAAAPPPR
                     FVDESSEAGIDHVYDGEFEYFVGGGVAVLDCDEDGLSDMYLAGGANPSGLYHNESAVG
                     GALRFRQLLDEPSSNLTGVTGAYPLDIDSDEHADLAVLRFGEDVLLRGRGDCTFERAN
                     ETWSFDARDDWTVGFSATWEGDGAMPTLAFGNYLAEDGQLTGTCADHYLFRPDGERYA
                     EPVTLSPGWCTLSVLFSDWDRSGRRDLRMTNDQHYYRDGQEQLWRVEPYEAPHLYTEE
                     EGWQPMQIWGMGIAGQDVTGDGLPEFFLTSQGDNKLQTLADGASQPNYVDIAYDRGLT
                     AHRPYVGDTAAPSTAWHPEFQDVNNDTLVDLFVSKGNVEAQPGYAVRDPNNLLIANPD
                     GTYTEMAEEAGIVDFARSRGAALADFNLDGMLDLIEVNRREPIKLWRNVGWGDAASPV
                     AMGNWVALRLEQPAPNVDGIGSWIEIKVGNRVVQREITVGGGHAGGQLGWAHFGAGDA
                     DSVEVRVQWPDGETGRWLNVSTNQMTIIERGADQAVAWDPSR"
     gene            1984..>2871
                     /locus_tag="VFU96_04285"
     CDS             1984..>2871
                     /locus_tag="VFU96_04285"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEU5112512.1"
                     /translation="MSGAHLTEVELPDFGMPTSMPVIPDSIYQDRMERLRKRAAERGL
                     DRIVVYADREHSANLSYLTGFDPRFEEALLVVGDDNEPAILVGNECWGMAGAAPLRMR
                     RHLYQDFSLPSQPRDRSLPLGDILGQEGIAAGSRVGVVGWKVFEDSGIIDLPAFIVDE
                     LRQLTGSAGAVENAADMLVGAADGLRVINEVEQLAYYEWAACHTSSGVRRVLEGIRPG
                     LTEQEAVRLLEWNGTPLSCHLMLTAGPRATLGLLSPGDRPIERGDRFTVAFGIWGALN
                     CRAGFVAEGPGDLPEEIDDY"
BASE COUNT          515 a          901 c          963 g          492 t
ORIGIN      
        1 aatggcccat tccggatgga gatgattggg caacggtatg tagacggcct ctatctcagg
       61 gtcagcaagc agctcctcgt agctcccgta ggaccgggga atgccgaggg tgtctgccgt
      121 tgcccgggcc cggtccgagg atcgcgaggc gatggcggtg acatggctga gctccgaccc
      181 gtgcatggcc gggatcacct tcttgaccgc gatatcggca cagccgatca cgccccatct
      241 gacggggtcc gtcacctcat agcctcctcc attcgacaga atctgcccat gcggcattgt
      301 gtcgaaacca cccatcaggg gcaaacatga ccggccgggc ctgggcctgg ctcgtcgccg
      361 gtgtcgccgc gggggcactc gtggccgggg cactcgccat gggagccgtc gccgttttcg
      421 gttccgacct tcccgatgcg gctgcgccgc ctccccgctt cgtcgacgag tcgtcggaag
      481 ccggcatcga ccatgtctac gacggcgaat tcgagtattt cgtcgggggc ggggtcgcag
      541 tgctcgactg tgacgaggat gggttgtccg acatgtactt ggcgggagga gccaatccca
      601 gcgggctcta tcacaacgaa agcgcggtcg gtggcgccct tcgattcagg cagttgttgg
      661 acgagccttc cagcaatctc accggtgtga ccggggccta ccccctcgac atcgacagcg
      721 acgaacacgc cgacctggca gtgctccgat tcggcgaaga cgtgttgctc cgcggccgag
      781 gggattgcac cttcgagcgc gccaacgaga cctggtcctt cgatgcacga gatgattgga
      841 ccgtaggctt cagcgccaca tgggagggcg atggcgccat gccgacgctc gccttcggca
      901 actacctggc cgaggacggc cagctgaccg gcacctgtgc cgaccactat ctatttcgcc
      961 cagatggcga acgatatgcg gagccggtca ccctctcccc aggctggtgc accctctcgg
     1021 tcctgttcag cgactgggac cgatcgggtc gtcgcgatct gcgcatgacc aacgaccagc
     1081 actactaccg ggacggacag gaacagctct ggcgcgtcga gccatacgaa gcgccacatc
     1141 tgtacaccga ggaggaaggc tggcaaccaa tgcagatctg gggcatgggt atcgccggcc
     1201 aggacgtgac cggcgacggg ctccccgagt tctttctcac cagtcagggg gacaacaagt
     1261 tgcagacttt ggcggacggg gcttcgcagc caaactacgt cgacatcgcc tacgaccgcg
     1321 gcctgaccgc ccatcgaccg tatgtgggcg acacggcggc gccttcgacc gcctggcatc
     1381 ccgaattcca ggacgtcaac aacgacaccc tcgtcgacct gttcgtgtcc aagggaaatg
     1441 tcgaggcgca acctggttat gcggtcaggg accccaacaa cctcctcatc gccaacccgg
     1501 atggcaccta caccgaaatg gcagaggagg cagggatcgt cgatttcgct cgatcacggg
     1561 gcgccgccct cgccgacttc aatctcgatg gcatgctcga tctcatcgag gtcaaccggc
     1621 gcgagcccat caagctctgg cgaaatgtcg gatggggtga tgccgcctca ccggtggcaa
     1681 tggggaattg ggttgccctt cggctggagc agccggcacc gaacgtcgac ggcatcgggt
     1741 catggatcga aataaaggtc gggaaccgag tcgtccaacg ggagatcacg gttggcggag
     1801 gccacgccgg cgggcagctg ggttgggcac acttcggcgc cggcgatgcg gatagcgttg
     1861 aggttcgggt tcagtggccg gatggagaga cggggcggtg gctcaacgtg agcacgaacc
     1921 agatgacgat catcgagcga ggtgccgacc aggccgtcgc gtgggacccg agccgatgaa
     1981 gcgatgagcg gggcacacct caccgaggtg gagttgccgg atttcggtat gcccacctcg
     2041 atgccggtca tccccgattc catttaccag gaccgcatgg aacgtctgcg caagcgggca
     2101 gcagagcgtg ggctcgaccg gatcgtggtg tatgccgacc gcgagcacag cgccaatctg
     2161 tcttatctca ccgggttcga ccccaggttc gaggaggccc tcttggtggt gggcgacgac
     2221 aatgagccgg cgatcctggt gggcaacgag tgctggggca tggcgggggc cgctcccctc
     2281 cggatgcgac ggcacctcta ccaggacttc agcctgccca gccagcctcg tgaccgatcg
     2341 ctccccctgg gcgacatcct cggacaggag ggcatcgcag ccgggagcag ggtgggggtg
     2401 gtgggatgga aggtgttcga agacagcggg atcatcgacc ttcccgcgtt catcgtcgac
     2461 gagctgcggc agctcaccgg atcggctgga gcagtcgaga atgctgcgga catgctggtc
     2521 ggtgcggccg acgggctccg tgtcatcaac gaggtcgaac agctcgccta ctacgaatgg
     2581 gcggcctgcc acacctcgag cggcgtgcgt cgcgtgctcg aggggattcg gcctggcctg
     2641 accgagcaag aggcggtccg gcttctcgag tggaacggaa ccccgctctc ctgtcacctc
     2701 atgctgacgg ccggcccgcg ggcgacgctc gggctgctca gccctggtga ccggcccatc
     2761 gagcgcggtg accgtttcac cgtggcgttt gggatctggg gagccctcaa ctgtcgggcc
     2821 gggttcgtgg cggaaggccc gggtgacctg ccggaggaga tcgacgacta t
//
LOCUS       DASTXO010000203         2124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1522602, whole genome shotgun sequence.
ACCESSION   DASTXO010000203 DASTXO010000000
VERSION     DASTXO010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522602"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1315)
                     /gene="hydA"
                     /locus_tag="VFU96_04290"
     CDS             complement(<1..1315)
                     /gene="hydA"
                     /locus_tag="VFU96_04290"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEU5112513.1"
                     /translation="MARTLIKGGTVVTATETMTADVLVDGEKVVAIAAPGSHDWESGA
                     ETTIDASGQYVLPGGIDVHTHMELPFGGTFASDNFETGTRAAAFGGTTTIIDFAVQTK
                     GGSAMEGYESWREKADGKVAIDYGLHMITGDINEQTLKEMDVLVDEGVTSFKLFMAYP
                     GVFYSDDGEILLAMQKAAENGSTICMHAENGIAIDVYVEQALKRGQTDPLYHGLVRKA
                     LLEGEAAHRAIVLAELTGAPLYIVHTSAKEAVQEVMNARDRGLNVFAETCPQYLFFDL
                     DNLGNGFEGAKYVCSPPMRDRAAGHQDALWQGLRTNDLQVVSTDHCPFCFNDHPELGR
                     QKRLGEEDFSKIPNGMPGVEPRMEVLFEGGVNQGRIGVNRFVEITATTPAKMFGMYPR
                     KGTVAPGGDADIVVFDPNEKHTISVDNQHMRVDYSAYEGMEVTGKV"
     gene            complement(1331..>2124)
                     /locus_tag="VFU96_04295"
     CDS             complement(1331..>2124)
                     /locus_tag="VFU96_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012378401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEU5112514.1"
                     /translation="KDSMIKKNADYAREAAQQGAQVMCFQELFYGPYFCQVQENEHFE
                     YAEPIPDGPTTQLMQDLAKETGMVLVVPIFEKEQDGFYYNTAAVIDADGSYLGKYRKT
                     HIPHVKGFWEKFYFRPGNTGYPVFDTAVGKVGVYICYDRHFPEGWRALGLNGARIVFN
                     PSATSRGLSMYLWNLEQPASAVANEYFVGAINRVGQEPHGDNDFYGSSYFVDPRGQVV
                     GGAASDTEEELVIRDLDLDMIEEVRQQWAFYRDRRPDAYGDLVAP"
BASE COUNT          357 a          686 c          653 g          428 t
ORIGIN      
        1 tgaccttccc ggtcacctcc atcccctcgt aggcggagta gtcgacccgc atgtgctggt
       61 tgtccaccga gatggtgtgc ttctcgtttg gatcgaagac aacgatgtcg gcgtccccgc
      121 ccggggccac cgtccccttc ctggggtaca tgccgaacat cttggccggg gtggtggcgg
      181 tgatctcgac gaaccggttg accccgatcc ggccctggtt tacgccaccc tcgaagagca
      241 cctccatacg gggctcgacg ccgggcatgc cgttggggat cttggagaag tcctcctcgc
      301 cgagtcgctt ctgccgaccc aattccggat gatcgttgaa gcagaacggg caatgatcgg
      361 tcgagaccac ttggaggtcg ttggtccgca gcccttgcca gagggcgtcc tggtggcccg
      421 ccgcccgatc tcgcatcggg ggtgagcaga cgtacttcgc tccctcgaat ccgttgccca
      481 gattgtctag gtcgaagaag aggtactggg gacaggtctc ggcgaacacg ttgaggccgc
      541 ggtcccgtgc gttcatcacc tcttgcacgg cctccttggc cgaggtgtgg acgatgtaga
      601 gcggagcgcc ggtgagctcg gcgaggacga tggcgcgatg agccgcctct ccctcgagga
      661 gggccttgcg gacgagcccg tggtagagcg ggtcggtctg gccccgcttc agcgcctgtt
      721 cgacgtagac gtcgatagct attccattct cggcgtgcat acagatggtc gacccgttct
      781 ccgccgcctt ctgcatggcg agcaggatct ccccgtcgtc cgagtagaaa acccccgggt
      841 aggccatgaa cagcttgaac gaggtcacac cctcgtccac cagaacgtcc atctccttga
      901 gcgtctgctc gttgatgtcg ccggtgatca tgtggaggcc atagtcgatg gcgaccttgc
      961 catcggcctt ctcccgccag gactcgtaac cctccatcgc cgagccgccc ttggtctgga
     1021 cggcgaagtc gatgatcgtg gtagtcccgc caaaggctgc cgcccgggtc ccggtctcga
     1081 agttgtccga agcgaaagtg ccgccgaagg gaagctccat atgggtgtgg acgtcgatcc
     1141 ctcccgggag cacatattgg ccggaggcat cgatggtggt ttcggcgccg gactcccagt
     1201 cgtgtgagcc cggtgcggcg atggccacca ctttctcccc gtcgaccagc acatcggcag
     1261 tcatcgtctc ggtcgcggtg acgaccgtcc cacccttgat cagcgtccta gccatgacca
     1321 acctccttct tcagggggct accaggtcac cgtatgcgtc agggcgccgg tcccgataga
     1381 aggcccactg ctggcgaact tcctcgatca tgtccagatc caggtcccgg atcacgagct
     1441 cctcttcggt atcagaagcc gcacctccga cgacctgacc ccgcgggtcg acgaaatagg
     1501 acgagccgta gaagtcgttg tctccgtggg gctcctgacc gacgcggttg atggcgccta
     1561 cgaagtactc attggccacc gccgaggccg gctgctcgag gttccacagg tacatcgaga
     1621 gtccccgact cgtggcggag gggttgaaga cgatcctcgc cccgttgagc cccagcgccc
     1681 gccatccctc cggaaagtgg cggtcgtagc agatgtaaac accgaccttc ccgactgcgg
     1741 tgtcgaacac cgggtatccg gtgttgccgg gccggaagta gaacttctcc cagaagccct
     1801 tgacgtgggg gatgtgcgtc ttgcggtact tacccagata ggagccatcg gcgtcgatca
     1861 ccgccgccgt gttgtagtag aagccgtcct gctccttctc gaagatcggg acgacgagca
     1921 ccattcccgt ctccttggca aggtcctgca tcaactgggt ggttgggccg tccgggatcg
     1981 gctcggcgta ctcgaagtgc tcgttctcct gtacctggca gaagtagggg ccgtagaaca
     2041 actcctgaaa gcacatcacc tgcgctccct gctgcgccgc ctcgcgggcg tagtccgcgt
     2101 tcttcttgat catcgagtcc ttgt
//
LOCUS       DASTXO010000204         3111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_676058, whole genome shotgun sequence.
ACCESSION   DASTXO010000204 DASTXO010000000
VERSION     DASTXO010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676058"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VFU96_04300"
     CDS             complement(<1..253)
                     /locus_tag="VFU96_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112515.1"
                     /translation="MTRAGLIDQLSAFSAALRNRGLTVTADQTTDMARALSMIDLSRR
                     TQAEAALRSLTVTDPDQVPIFHREFEIFFGRLLPPIWVDD"
     gene            complement(253..1128)
                     /locus_tag="VFU96_04305"
     CDS             complement(253..1128)
                     /locus_tag="VFU96_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU5112516.1"
                     /translation="MIPDTIGELEKRLDDQDYFADESLVVAIHLAVQLGRPLFLEGEA
                     GVGKTEVAKVLAALGQTELIRLQCYEGLDVGQALYEWDYARQMMAIRLLEARGEGATA
                     TPDDIMSRDYLIARPLLQAIESSKEAHRPVLLIDELDRADEEFEAFLLELLSDFQITI
                     PEIGTIKALSRPIVVLTSNRTREIHDALKRRCVYHWIDYPSPAKEAEIILRKVPELEG
                     RLAATLAEAMRRLRDMELFKPPGVAETLDWAESLRLLQAGELTPATIDSTIGVVLKYK
                     DDIDMVRSAGVDSLV"
     gene            1164..2087
                     /locus_tag="VFU96_04310"
     CDS             1164..2087
                     /locus_tag="VFU96_04310"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEU5112517.1"
                     /translation="MYGVALTVLACLRSGTRADVAWLLKAEGLPVADWSDTVVFTPGG
                     GRIGSMAGGALDGQLADQAGRSDTGRLVDIEISEIDALIAGLPSTGSAKCLLVPAVAF
                     PEELWDLAAAREPVCLVCVLEGDEVTAIDLYTTSTVVDAGESVETLFNAGQSGSTVTE
                     DLVVSVFRAVPQLVVVGDTDVAGALVELASRIGWNARVAADAASATGMIATLSGQDKV
                     VVAAHDLELAGTALMAALESDVGYIGALGARRMQENRADWLAYRGVTDLTRIHGPAGL
                     DIGADTPAEIAVSILAEAIAENAGDHAVSDG"
     gene            2163..2414
                     /locus_tag="VFU96_04315"
     CDS             2163..2414
                     /locus_tag="VFU96_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112518.1"
                     /translation="MTAPHETTADALKSIWISSHDHDPLDEECSPTKVCRNMKDVTFD
                     GYAEPGTDLGAAWRAKLAREGKLSTSGASVRDLILGQGE"
     gene            2497..>3111
                     /locus_tag="VFU96_04320"
     CDS             2497..>3111
                     /locus_tag="VFU96_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU5112519.1"
                     /translation="MIARLGEGLYLADRGLATAVYVALTLGRPLLLEGEVGVGKTEVA
                     KVLATVLQRQLIRLQCYEGIDTNQALYEWDYARQMLQIRALSERDLADEDHVDKLFGP
                     KFLLERPLLTAVREGDRAVLLIDEIDRADDEFEAFLLELLSDYQITIPEIGTISAAAP
                     PLVILTSNRTRELHDALKRRCLYHWVGYPSAEREVEIILVRAPGA"
BASE COUNT          521 a          962 c         1083 g          545 t
ORIGIN      
        1 tgtcgtcgac ccagatcggg ggtagcagcc ggccgaagaa gatttcgaac tcccgatgga
       61 agatcgggac ctgatccggg tcggtgacag tcagtgagcg cagcgccgct tccgcttgtg
      121 tccggcggct gagatcgatc atggagaggg cgcgggccat gtcggtggtc tggtcggcgg
      181 tcacggtgag gccccggttg cgcagggcgg ctgagaaggc gctcagctgg tcgatcaggc
      241 cggcccgggt catcacacca ggctgtcgac cccggcggag cgaaccatgt cgatgtcgtc
      301 cttgtatttg aggacgacac cgatggtgct gtcgattgtc gccggtgtca gctcgccggc
      361 ctgcagcaac cgcagcgact cggcccaatc gagcgtctcg gcgacacccg ggggcttgaa
      421 gagctccatg tccctcagcc tgcgcatcgc ctcggccagc gttgccgcga gacggccctc
      481 cagctccggc accttgcgga ggatgatctc ggcctccttg gccggggacg ggtagtcgat
      541 ccagtggtag acacagcgac gcttcagggc gtcgtggatc tcccgggttc ggttcgaagt
      601 gaggaccacg atcggcctgc tcagtgcctt gatggtcccg atctcgggga tcgtgatctg
      661 gaaatcagag agcagctcga gcaggaatgc ctcgaactcc tcgtccgccc ggtcgagctc
      721 gtcgatcagg aggacgggcc ggtgcgcctc cttgctggat tcgatcgcct ggaggagtgg
      781 ccgggcgatc aggtaatccc gactcatgat gtcgtcggga gtggcggtgg caccctcacc
      841 tctcgcctcc aggaggcgaa tcgccatcat ctggcgggcg tagtcccact catagagagc
      901 ctgccccacg tcgagcccct cgtagcactg gagtcggatg agctcggtct gtcccagggc
      961 tgccagcacc ttggccacct cggtcttgcc gactcctgct tccccctcga ggaagagggg
     1021 tctccccagc tggacggcga ggtggatggc gacgacgagg ctctcatcgg cgaaatagtc
     1081 ctggtcgtcg agacgcttct cgagctcgcc gatggtgtcc gggatcacag gaggaagcct
     1141 aaacggcgcc gtacaatcga tttgtgtacg gggtggcgct caccgttctc gcttgtttgc
     1201 gctcggggac ccgcgccgac gtggcgtggc tcctcaaggc ggaaggattg ccggtcgcag
     1261 actggtccga cacggtcgtg ttcacgcccg gcggcggccg gatcgggtcg atggcaggcg
     1321 gcgccctcga tgggcagctg gcggaccaag ccggtcgatc ggatacgggg cggttggtcg
     1381 acatcgagat cagcgagatc gacgcgttga tcgccggctt gccctcgacg ggctcggcga
     1441 aatgccttct cgtccccgcc gttgcatttc ccgaagagct gtgggatctg gcagccgcgc
     1501 gggagccggt ctgtctggtg tgcgtgctcg aaggcgacga ggtgacggcc atcgacctgt
     1561 atacgacgag cactgtggtc gatgccgggg aatccgtgga gaccctgttc aacgcgggcc
     1621 agagcggctc gacggtgacc gaagatctcg tggtctcggt gttcagggcg gtgccgcagc
     1681 ttgtcgtcgt aggcgacacc gacgtggccg gagcgttggt cgagctggcg tcccgcatcg
     1741 ggtggaatgc ccgggtcgcc gccgatgcag cctcggccac agggatgatc gcgacgctgt
     1801 ccggtcagga caaggtcgtg gtggcggcac atgatctcga gttggccggg accgcactca
     1861 tggctgcgct ggagagcgat gtcggctaca tcggggcgct gggagcgcgc cggatgcagg
     1921 agaacagggc cgactggctc gcctatcggg gagtgaccga tctgacgcgg atccatggcc
     1981 cggccggcct cgatatcggc gccgacacac cggccgagat cgcggtttcg atcctggccg
     2041 aggcgattgc ggaaaatgcc ggagaccatg cggtttcgga tgggtagccg tggagtaccc
     2101 gggggccctc cactacactc gatggctgac gatcacaggc gagaacttcc cgggagaact
     2161 ggatgaccgc cccgcacgag accacggctg acgctctgaa gtcgatctgg atcagcagtc
     2221 acgaccatga cccgctcgac gaggaatgca gcccgaccaa ggtctgccgc aacatgaaag
     2281 acgtgacctt cgacggttac gccgagcccg gcacggacct gggagccgcc tggcgggcaa
     2341 agctggcccg ggaggggaag ctgtcgacct ctggggcgag cgtccgggac ctgatcctcg
     2401 gtcagggcga atagccctgg gcgcatcaac gaccacccac gcgcacgggg tcacttcgtg
     2461 agcaactacc cggcaccacc cggcagcgtc gacgaggtga tcgcacgtct cggcgagggt
     2521 ctctacctgg cagatcgagg gttggcgacg gccgtgtacg tggccttgac actgggccgg
     2581 ccgcttcttc tcgagggcga ggttggtgtg ggcaagaccg aggtggccaa ggtcctcgcg
     2641 acggtcctgc agaggcaact gatccgcctg cagtgttatg aggggataga caccaaccag
     2701 gccctctacg agtgggacta cgcccgccag atgctccaga tcagggcgct gtccgagcgt
     2761 gacctcgccg acgaagacca cgtcgacaag ttgttcggcc ccaagttctt gctcgagcgg
     2821 cccctgctga cggcggttcg ggaaggtgac cgcgcggtgc tgctcatcga cgagatcgat
     2881 cgggccgacg atgagttcga ggcgtttttg ctcgagttgt tgtccgatta tcagatcacg
     2941 atcccggaga tcggcacgat ctccgcagcc gccccgccgc tcgtcatcct cacctcgaac
     3001 cgcacgcgtg agctccacga cgccctgaag aggcgatgcc tgtaccactg ggtcgggtat
     3061 ccgagcgccg agcgcgaggt cgagatcatc ctggttcgag ccccgggtgc c
//
LOCUS       DASTXO010000205         3810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_933538, whole genome shotgun sequence.
ACCESSION   DASTXO010000205 DASTXO010000000
VERSION     DASTXO010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933538"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VFU96_04325"
     CDS             <1..772
                     /locus_tag="VFU96_04325"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEU5112520.1"
                     /translation="LAVGAYVPWARGGVGAVATQAHAEITYGNRGLQMLESGLTPDVV
                     IEGLVTHDEMRSHRQVGIVNADGASATYTGEDCFEHAVGVSGENFAAQGNILSNPDVP
                     EAMAAAFLGSSGRLVDRLFAALAAGEAAGGEKRGMESAALLVVRPSGGYGGNHDRWLD
                     LRIDHSDSPIEDLGGLIDLHTLYFGRTDPGDLLAFDDVLQTEVRQLLDAVGWWDASES
                     LGDNLQNWLGWHNLEERWVSESLIDPVVLGELRKTATA"
     gene            783..1748
                     /locus_tag="VFU96_04330"
     CDS             783..1748
                     /locus_tag="VFU96_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018159742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HEU5112521.1"
                     /translation="MIVDAACYQLGQRIPGGFDYDEAGRLITEPDTFAWVGLHEPSPE
                     EFEQAVKTFHLHELAVDDALRAHQRPKLETFDDTLFVVTKPARYADPVEIIEFGEILI
                     FVDPDFAVAVRHGEASRLIEARHKLEADPGRLACGSGSVLHAILNQVVRDYAVVLDEL
                     GADIRGIEDDVFSSKGDSERIYRLKQEVLSFHEAVEPLEEPLDLLATEDFKAIDPSLK
                     PYFRDVLDHLLRVLDRINIYRDTLTSVLAANLTQVSLRQNEDMRKISAYVAIAAIPTL
                     LAGIWGMNFEHMPEISAVWGYPAALLVMGGVCLFLYTRFKKSGWL"
     gene            2026..2586
                     /locus_tag="VFU96_04335"
     CDS             2026..2586
                     /locus_tag="VFU96_04335"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEU5112522.1"
                     /translation="MTTTDTPVNNGVNVQALLDARNALVDAPEAAQFTWKASTEWKGG
                     THSHSVVDEFFGLGDDQTHRTTFTFDADHPEVFASEDNGATPVEFVLVGLASCLTAGV
                     AAVAQNRGIQLNSVKATIEGDMDILGILGADPEVRNGFNHIKVKYDIDADASQEDIEA
                     LVAQSQKRSAVFDVITNPTHVTVEVN"
     gene            2616..>3810
                     /locus_tag="VFU96_04340"
     CDS             2616..>3810
                     /locus_tag="VFU96_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015794823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEU5112523.1"
                     /translation="MIGAGHAGLAVSHFLSQRSIDHIVIERGEVANTWRTERWDSLRL
                     LTPNWQSRLPGLEYDGDDPDGYMTMPEVIEFIEAFAKTVTPPLRTHTTVSSLRPNHDG
                     YEVVTDDGIWSAPTVVLATGGFNLANVPALSDAVPQSVETVTPLQYRRPDQLPEGGVL
                     VVGASATGIQIAEEVQVSGRPVTLAVGEHVRLPRVYRGKDIMWWLDRTGVLNERWDEV
                     DDLVRARNIPSPQLVGTPERRTLDLNALTDQSVRLVGRVAGIVDGTVQLSGSLRNVCN
                     LADLKMDRLLGTIDEWVLVNGLEGEVEPSHRFAPTRVEESPPLSLDLTDGRISTVIWA
                     TGFRPDYSWLHARVLDRKGRLRHDAGVVTGAPGMYRIGLNMLRRRKSSFIHGAEDDAR
                     DLTDHL"
BASE COUNT          721 a         1179 c         1221 g          689 t
ORIGIN      
        1 cctcgccgtc ggtgcatatg tgccctgggc gcgaggtggg gtgggcgcgg tcgccaccca
       61 ggctcacgcc gagatcacct acggaaaccg cggcctgcaa atgctcgaat ccgggctcac
      121 cccggacgtc gtgatagagg gcctcgtcac ccacgatgag atgcgaagcc atcgtcaggt
      181 cgggatcgtg aatgcggacg gggccagcgc cacatacacg ggggaggact gcttcgagca
      241 cgcggtaggc gtctcggggg agaatttcgc cgcccagggc aacatcctct ccaacccgga
      301 cgtccccgag gcgatggccg cagcattcct cggatcgtcg ggacggctcg tcgatcgact
      361 cttcgcggcc ttggcggccg gggaggcggc cgggggggag aagcgcggca tggaatcggc
      421 ggcgctactc gtggtcaggc ccagtggcgg ctacgggggg aaccacgacc ggtggctgga
      481 cctgcgcatc gatcactccg atagcccgat cgaggatctg ggcggtctca tcgatctcca
      541 cactctgtat ttcggtagaa ccgacccggg agacctcctg gcattcgacg acgtcttgca
      601 gaccgaggtt cgacagctcc ttgacgccgt cggatggtgg gatgcgagtg agtcgctggg
      661 ggacaacctc caaaactggc tgggctggca caacctcgag gagcgatggg tgagcgagag
      721 cttgatcgac cctgtcgtcc tgggcgagct tcgcaagact gcgaccgcgt agcctgcacg
      781 gcatgatcgt cgatgctgcc tgctatcagc ttggacagag gattcccggc ggattcgact
      841 acgacgaggc ggggaggctc atcaccgagc ccgacacgtt tgcctgggtg ggtctgcatg
      901 agccgagccc ggaggagttc gagcaggccg tcaagacttt tcaccttcat gagctggcgg
      961 tcgacgacgc gttgcgcgcc caccagagac caaagctgga gaccttcgac gacacccttt
     1021 tcgtcgtgac caagccggcg cggtatgccg acccggtcga gatcatcgaa ttcggcgaga
     1081 tcctcatctt cgtcgacccg gacttcgcgg tcgccgtccg tcatggcgag gccagccgac
     1141 tgatcgaggc ccggcacaag ctcgaagcgg atccaggacg gctcgcctgc ggctccggtt
     1201 ccgtccttca cgccattctc aatcaggtgg tgagggacta cgccgtggtc ctcgacgaac
     1261 tgggggccga cattcgcggc atcgaggacg atgtcttctc ctcgaagggt gactctgagc
     1321 gcatctaccg gctcaagcag gaggtgctca gttttcacga ggcggtagag cccctagagg
     1381 agccgttgga cctgctggcc acggaggact tcaaggccat cgacccaagc ctgaagccct
     1441 atttccgtga tgtgctcgac cacctccttc gggtgctgga ccggatcaac atctaccggg
     1501 acacattgac ctcggttctg gccgccaacc tgacccaggt cagccttcgt cagaacgaag
     1561 acatgcgcaa gatctctgct tacgtcgcaa tcgcagccat acccactttg ttggccggga
     1621 tctgggggat gaacttcgag cacatgcccg agatcagtgc cgtgtgggga tatccggccg
     1681 cgcttctggt gatgggcggc gtgtgcctgt ttctctatac ccggttcaag aagagcggct
     1741 ggctctgacc gacgcatggg ggcgattccc catgctcatc ctttgacgca tcgacggctc
     1801 cctgctcaaa ttgtcggtga gctcgtcagc agctgaaagc ggagccatga caggaacgca
     1861 caacatcgaa aatcgcccgg ccgggcccat gtgcatctgt cttcgctctc gggcggccgt
     1921 gacgagcgac atcattcgct cgtagggatg tcaggctccc gagagtcatc gagaagatct
     1981 cgagacgtcc tgctattcga gaagcgacca gatcaggagg aagaaatgac cacgaccgac
     2041 acaccggtca acaacggagt gaacgtacaa gcgttgcttg atgcacgaaa tgccctcgtc
     2101 gatgccccgg aagctgccca attcacctgg aaggcatcca ccgaatggaa gggtgggacc
     2161 catagtcact ccgtggtgga tgagttcttc ggtctcggcg acgatcagac ccatcggacg
     2221 acattcacct tcgatgccga tcaccccgaa gtgttcgcat ctgaggacaa cggggccaca
     2281 ccggtcgaat tcgtcctcgt ggggctggcc agctgcctga ccgccggcgt cgccgcagtg
     2341 gcgcagaacc ggggcatcca gctcaattcg gtgaaggcga cgatcgaggg cgacatggac
     2401 atccttggaa tcctcggtgc tgatcccgag gtgcgcaacg ggttcaacca catcaaggtg
     2461 aagtacgaca tcgacgccga tgcgtcgcag gaagacatcg aggcattggt cgcacagtcc
     2521 cagaagcggt cggcggtgtt cgacgtgatc accaacccga cccacgtgac tgtcgaagtc
     2581 aactgagcaa aaagaccata cgcactacga cggtggtgat cggcgctggt cacgccgggc
     2641 tcgccgtcag ccatttcctc agccagcgct cgatcgatca catcgtcatc gagcgcggcg
     2701 aggtggccaa cacctggcgc accgagcgtt gggactcgct gcggctgctc acgccgaact
     2761 ggcagagccg gctacccggc ctcgagtacg acggtgacga cccggatggc tacatgacga
     2821 tgcccgaggt gatcgaattc atcgaggcct tcgccaagac ggtcacgccg ccgctgcgga
     2881 cccacaccac cgtcagctcg cttcgaccca atcacgacgg atacgaagtc gtgacggatg
     2941 acgggatttg gtccgctccg actgtggtcc tcgcaaccgg tggtttcaac ctggccaacg
     3001 tccctgccct ctccgacgcg gtgccccagt cggtggagac cgtcacaccg ctgcaatacc
     3061 gccggcccga tcagcttccc gaaggcggtg ttctggtggt cggggcttcg gcgacgggta
     3121 ttcagatcgc cgaggaagtg caggtctcgg gtcggccggt gacattggcc gtcggcgagc
     3181 acgtccgact gcctcgggtg tatcgaggca aggacatcat gtggtggctg gataggaccg
     3241 gagtgctcaa cgagcggtgg gatgaggtcg acgatttggt ccgtgcccgc aacatcccat
     3301 cgccgcagct ggtgggaacg cccgagcgca ggaccctgga cctcaacgcc ttgaccgacc
     3361 agagtgtgcg actagtcggc cgggtcgccg gcatcgtcga tgggaccgtc cagctctccg
     3421 gttctcttcg caacgtctgc aatctcgccg acctcaagat ggatcgtctg ctcggcacta
     3481 tcgacgagtg ggtgctggtg aacgggttgg agggcgaggt cgagccatct catcggttcg
     3541 ccccgacacg agtcgaggag tcgccaccgc tcagcctgga cctaacggac ggccggatct
     3601 cgaccgtcat ctgggcgact ggttttcgtc ccgattactc gtggctccat gcgcgcgtcc
     3661 tcgaccgtaa gggacggctg aggcacgacg caggggtggt cacgggggct cccggcatgt
     3721 atcggatcgg gctgaacatg ttgcgtcgtc ggaagtcgag cttcatccac ggcgctgagg
     3781 acgatgctcg ggatttgacc gaccaccttg
//
LOCUS       DASTXO010000206         2964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_49508, whole genome shotgun sequence.
ACCESSION   DASTXO010000206 DASTXO010000000
VERSION     DASTXO010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49508"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VFU96_04345"
     CDS             <1..241
                     /locus_tag="VFU96_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112524.1"
                     /translation="HDHLEGSPMNLAIGFPCPKCGHEEANLLAFERNRNAEVVGLRLL
                     CMKCESVFRDNGLVDEAVPADVSAHSGKGARSWVA"
     gene            complement(270..1220)
                     /locus_tag="VFU96_04350"
     CDS             complement(270..1220)
                     /locus_tag="VFU96_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5112525.1"
                     /translation="MGKAIRAKDARPERGSSPADTVGLYLDEVSAHLLLTAEDEVHLS
                     RSMELGKKAKATLESGDYDKADRVKLMRLVAEGDEAKMTFIRSNLRLVISIAKRYTGR
                     GLDLLDLIQEGNLGLIRAVEKFDWRKGFKFSTYATWWIRQAITRGLGNNGKTIRLPVH
                     MVDVVRTVQDTRQSLHDKYHRAPTLEEISEASGLDTERVLAALEAPAETVSLDRPVGE
                     DGDASLQDFVEDATAVDPEARAAAAWRRDRLIKALQILDPEEQQVLWLRFGLDGDEPW
                     TLSDVGKVINSTRERVRQIEARGLAKLRHPSCDIDLQALL"
     gene            1356..2786
                     /locus_tag="VFU96_04355"
     CDS             1356..2786
                     /locus_tag="VFU96_04355"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEU5112526.1"
                     /translation="MALTIVWALWAGWSLLGRATPYTLRVLDDAGAPIAAASVDINES
                     QVGTSADDGTIEVEWNRSSTVLEVSAAGHVPKTVTISERPDQVFEVVLKARILRGRVL
                     DPAGRPVEAAVVVAGPASGTTDVEGKISRRGAEPGSVEVSRPAWIPASFEWDGGAGES
                     VVELAPFVARAVHIAGEAAAEDIDHFFEMANTTELNALMLDLKDESGLVWYNSVDPTA
                     DEVGAIRGVYDLNAVAARADEEDLYLIGRLVVFNDPTAALRKPDMSVWDSATNEPYQS
                     NGQYFLDPTDPNARAYGLALAQEACSMGVDEVQFDYIRFPDKRSESTTFDQGVSLETR
                     TATIIGFLEEAVALLHPMGCAVGADVFGFTTAAADDGGIGQNWEQVTQVVDVASPMVY
                     PSHYTSGWYDFETPNDHPGPMVTYAIQDGMERLARNVVVRPWLQDFGYTPEQVRAQIE
                     VAEEYGLGWMLWNAESNVTVDALRAE"
BASE COUNT          528 a          893 c          976 g          567 t
ORIGIN      
        1 ccacgaccat ctggagggat caccaatgaa tcttgccatc ggattcccct gccccaaatg
       61 tgggcacgag gaagccaatc tcctcgcatt cgagaggaat cgaaacgccg aagtggtcgg
      121 ccttcgactc ctctgcatga agtgcgaatc ggtgttccgt gacaacggtc tcgtcgatga
      181 agcggtgccg gccgacgtgt cggcacacag cgggaaagga gcccgatcgt gggttgcatg
      241 agcaacgacc cgctggtcgt cggcgaccgt tagagcaagg cctgtaggtc gatgtcgcag
      301 ctgggatggc gcagtttggc cagccctctg gcttcgatct gacggacccg ctcccgggtg
      361 gagttgatga ccttgcccac atcggacagg gtccagggct cgtctccatc cagcccgaag
      421 cgcagccaca ggacctgttg ttcctcaggg tcgaggatct gaagggcctt gatgagccgg
      481 tcccggcgcc atgccgccgc ggcccgggcc tccgggtcga cggcggtcgc atcctcgacg
      541 aaatcctgca gtgaggcatc gccgtcctca cccaccggcc gatcgaggga gactgtttcc
      601 gccggtgcct cgagggcggc gagaaccctt tccgtgtcca agccggacgc ctcggagatc
      661 tcctcgagcg tcggggcgcg gtggtacttg tcgtggaggc tctgacgcgt gtcctgcacg
      721 gtgcggacca cgtccaccat atgaaccggc aggcggatgg tcttgccgtt gttgcccaat
      781 ccccgtgtga tcgcctgtcg gatccaccag gtggcgtagg tggagaattt gaagcctttc
      841 cgccaatcga acttctccac agcccggatc aatcccaggt ttccctcttg gatcaggtcg
      901 aggaggtcga ggcccctgcc ggtgtatcgc ttggcgatcg agatcaccag acgcaggttg
      961 gagcggatga aggtcatctt ggcctcgtcg ccctcggcga cgagacgcat cagcttgacg
     1021 cggtcggctt tgtcgtaatc gcctgactcc agggtcgcct ttgccttttt ccccaactcc
     1081 atggagcggg agagatggac ttcgtcctcg gcggtgagga ggagatgggc ggagacctcg
     1141 tcgagataca gaccgacggt gtcggcggga ctcgagcccc gctcgggtct ggcgtctttg
     1201 gcccttatgg ccttccccaa atctggccct cctgtcaaac ccctgtggaa aactaacgtt
     1261 gctggctgaa tctggcaaac ggttttccgt ttaacggctg gtgaacaagt aatctcggtc
     1321 gctcagtggc gcgtcgttcc cagtttcgct tgctggtggc gctcaccatc gtttgggcat
     1381 tgtgggcagg ctggtcgctg cttggacgtg cgactccata caccctccgg gtgctcgacg
     1441 acgccggagc gccgatcgcg gcggcgtcgg tcgacataaa cgagagccag gttggaacca
     1501 gcgccgacga cgggaccatc gaggtcgaat ggaaccggtc gtccacagtc ctcgaggtga
     1561 gtgccgccgg gcatgtcccc aagacggtga cgatttcgga gcgtcctgac caggttttcg
     1621 aggtcgttct caaggcgcgg attcttcgcg gccgtgtgct cgacccggca ggcaggcccg
     1681 tcgaggcggc cgtggtcgtg gcaggcccgg cttcggggac gacggacgtc gaggggaaga
     1741 taagccgtcg tggcgcggag ccggggagcg ttgaagtctc ccgcccggca tggatccccg
     1801 cctcattcga atgggatggg ggagccgggg aatccgtggt cgagctggcg ccattcgttg
     1861 cccgcgccgt ccacattgcc ggagaggccg ccgcggagga catcgatcac ttcttcgaga
     1921 tggcgaacac caccgagctc aatgccctca tgctcgacct caaagatgag agcgggctgg
     1981 tctggtacaa cagtgtggat ccgacggcgg acgaagtcgg ggcgatacgg ggcgtttacg
     2041 acctcaacgc cgtcgccgcc agggccgacg aggaggacct gtatctcatc ggtcggctcg
     2101 tggttttcaa cgacccgacg gctgcgctgc gaaaacccga catgtcggta tgggactcgg
     2161 cgactaacga gccctatcag tcgaacgggc agtatttcct cgaccccacc gacccgaacg
     2221 cgcgtgccta cggcctcgcc ctggctcagg aggcgtgctc gatgggggtg gacgaagtgc
     2281 agttcgatta catccggttt cccgacaaac ggtcagagag cacgacgttc gaccagggtg
     2341 tgagcctcga gacgaggaca gcgaccatca ttggatttct cgaggaggca gtcgccctgt
     2401 tgcatccgat gggttgcgcc gtcggcgcgg acgtattcgg cttcaccacc gcggccgccg
     2461 atgatggggg gatcggccaa aactgggaac aggtcacgca ggtggtcgat gtcgcctcgc
     2521 cgatggtgta tccgtcccac tacacgtcgg gttggtatga cttcgaaaca cccaacgacc
     2581 atccgggccc gatggtcacc tacgccatcc aggacgggat ggagcgcctg gcgaggaacg
     2641 tggtggtccg cccgtggctg caggatttcg gttacacgcc ggagcaggtt cgagcgcaga
     2701 tcgaggtcgc cgaggagtac ggcctgggtt ggatgttgtg gaacgccgaa tccaatgtca
     2761 cggtcgacgc tctccgggcg gagtaattcg cactatggcc ataggtgaaa taccattctg
     2821 tggtcccttc cggagcattg tttgaccgcg ttcgaccgag agaatctggc ccagatcacc
     2881 tcctcagccc cggtgtggag acactcccag atggaggctg cgtaccaggt cttcgctgga
     2941 ctagatgagc ccacaggggc ggag
//
LOCUS       DASTXO010000207         2635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1080738, whole genome shotgun sequence.
ACCESSION   DASTXO010000207 DASTXO010000000
VERSION     DASTXO010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080738"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="VFU96_04360"
     CDS             complement(<1..810)
                     /locus_tag="VFU96_04360"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU5112527.1"
                     /translation="MARLFASFMVFALMVAACGGDEGTEPDGTSAEGGESPEGATGAD
                     CALDEVDGDLNFYNWSEYIDPELVTAFEEQYQVDVIEDFYESNEALLAQMQAGAVYDL
                     IVPSDYMVGIMIENGLLAPLNRTAVPNVENLEARFTEVPYDPGNEYSAAYQYGTTGLG
                     VNKSVVGEDFPRSWALLFDPELTAEFPGGVSVLNDPREAMGAALKYLGYSLNDTDLEH
                     LQAAADTISAAKDSIATFDSDQYDEALVSGEVAVSHGYSGNMIVGIGEADNP"
     gene            complement(895..1776)
                     /locus_tag="VFU96_04365"
     CDS             complement(895..1776)
                     /locus_tag="VFU96_04365"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011612354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5112528.1"
                     /translation="MNEGTAPRGIVDRLLGLNAWVVYIFFYAPILLLIVFSFNDNNNV
                     GIWTQPSLRWYGEMFQDERVMGALRNSLVVAFFATIISTVLGTMLAIALERYRFRGRG
                     TLDGIAYLPIIIPDVTMAVMLLVFFFQAFGILGDLFGIQLRTGLLTISLAHIAFCISF
                     VAVVVRARLSQLDSSLEEAAADLYANRWQAFRKVTLPLIAPGVAGGALLALTLSLDDV
                     VVTQFVSGAGSTTLPVYVFGLVRRGVTPLINAVSTVMLVASMILIGISLGFQRGAEAR
                     GQKLADPEGLLEEITAD"
     gene            complement(1773..>2635)
                     /locus_tag="VFU96_04370"
     CDS             complement(1773..>2635)
                     /locus_tag="VFU96_04370"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5112529.1"
                     /translation="TPELEQAAERAEARRGFLISLPSFVYLILLFVVPLILVFVYSFA
                     TRDATGQTVLEGWNLDSYRRLFSTDVLEIFLRSMVLAVLTTLICLVICYPFAFYIATR
                     GTRVRNILLVLVMIPFWSNFLVRTYAWRVLLGSEGPLSQLSELLGGEPFRLLFTNVAV
                     LIGLVYGFMPFMVLPLYAALERMDWRLVEAARDLYADGWTAFRKVTLPLSMPGVVAGS
                     ILVFIPSLGAYVTPALLGGARTVLMGDYIVSQFLAARNWPFGSALSFAVMFVMLLATI
                     FYFRAGARTL"
BASE COUNT          561 a          817 c          841 g          416 t
ORIGIN      
        1 cgggttgtcc gcctcgccga tcccgacgat catgttgcct gagtagccgt gggagacggc
       61 gacctcgccg ctgaccagtg cttcgtcgta ctgatcggag tcgaacgtcg cgatcgaatc
      121 cttcgccgca gagatcgtgt ccgccgccgc ctgcagatgc tcgagatcgg tgtcgttgag
      181 cgagtagccg aggtacttca gggcagcacc cattgcctcc ctcgggtcgt tcagcaccga
      241 gaccccgccg gggaactccg ccgtgagctc gggatcgaac agcaacgccc agctgcgcgg
      301 gaagtcctcc ccgaccaccg acttgttgac gccgaggcct gtcgtcccgt actgataggc
      361 ggccgagtat tcgttacccg ggtcgtaggg aacctcggtg aaccgtgctt ccagattctc
      421 cacgttgggc acggcagttc tgttgagggg agcgagaagc ccgttttcga tcatgatgcc
      481 gaccatgtag tccgagggaa cgatgaggtc atagaccgcc ccggcctgca tctgggccag
      541 cagcgcctcg ttggactcgt agaaatcctc gatgacgtcg acctgatatt gctcctcgaa
      601 cgcggtcacc agttccgggt cgatgtactc ggaccagttg tagaagttga ggtcgccatc
      661 gacctcgtcg agcgcgcagt cggcgcccgt cgcgccctca ggcgactcgc caccctcggc
      721 actcgtgccg tcgggctcgg tgccctcatc accaccgcaa gccgcaacca tcagagcaaa
      781 caccatgaat gaggcgaaga gcctcgccat ccgtcgcatc gatcctcccg ggattccgcc
      841 ttgtgcccga caccttattc tcgtcccgtt caatgcgaga ggagccggtt cgactcaatc
      901 ggcagtgatc tcctcgagga gcccttccgg atctgccagc ttttggcccc gcgcctcggc
      961 gccgcgctgg aaccccaacg agatgccgat caggatcatc gaggccacga gcatcacggt
     1021 gctgaccgca ttgatcaacg gcgtcactcc ccttctcacc aggccgaaca cgtacacggg
     1081 gagcgtggtc gagccggccc cggacacgaa ctgggtcacc accacgtcgt cgagggacag
     1141 ggtcagggcg agcagggcac ccccagcgac gcctggggcg atcaagggca gagtcacctt
     1201 gcgaaaggct tgccagcggt tggcgtacag atcggcagcc gcctcttcga gcgagctgtc
     1261 gagctgggag agtcgagcgc ggacgaccac ggcgacgaaa gagatgcaaa aggcgatgtg
     1321 ggccagggaa atggtcaaca gcccggtccg gagctggatg ccgaagaggt cgccgagaat
     1381 cccgaaagcc tggaagaaga agacgaggag catcaccgcc atggtcacat cggggatgat
     1441 gatgggcagg taggcgatcc cgtcgagtgt tccccgtccc cggaaccggt atctctccag
     1501 agcgatggcc agcatggtgc ccaggacggt ggagatgatg gtggcgaaga aggcgacgac
     1561 cagggagttg cgcagagctc ccatcactcg ctcgtcctga aacatctctc cgtaccagcg
     1621 aagcgagggc tgggtccaga tcccgacgtt gttgttgtcg ttgaaggaaa acacgatcaa
     1681 caggaggatc ggtgcgtaga agaagatgta gaccacccag gcgttgaggc cgagcagccg
     1741 gtcgacgatc cctctgggag cggttccctc gttcacaacg tcctcgcccc ggcgcggaaa
     1801 tagaagatcg tcgccagcag catcacgaac atcaccgcga atgagagtgc ggagccaaat
     1861 ggccagttcc gagcggcgag gaattggctg acgatgtaat cccccatcag caccgtcctc
     1921 gccccgccga gcagcgcggg agtcacatag gcgccgagcg aggggatgaa gaccaggatc
     1981 gacccggcga ccactcccgg catcgacaac gggagcgtca ccttgcgaaa ggcggtccag
     2041 ccgtcggcgt agaggtcgcg cgctgcctcc accagtcgcc agtccatccg ctccagcgcc
     2101 gcatacagag gaaggaccat gaacggcatg aagccgtaga ccaggccgat gaggacggcg
     2161 acattggtga agagcaatcg gaacggctcg ccacccagca actcgctcag ctgcgacagg
     2221 ggcccctcag agccgagcag gactcgccat gcgtaggtcc tgaccaggaa attcgaccag
     2281 aaggggatca tcacgaggac gagcaggatg ttccggaccc gagtgcccct cgtcgcgatg
     2341 tagaacgcga acggataaca gatgaccagg cagatcagcg ttgtgagtac ggccaggacc
     2401 atcgacctga ggaagatctc caacacatcg gtggagaaga ggcggcggta ggagtcgagg
     2461 ttccagccct cgagcaccgt ctgtccggtg gcgtccctcg tggcgaagga atagacgaag
     2521 accaggatga ggggaacgac gaagagaagg atgaggtaga cgaacgaggg gagggagatg
     2581 agaaagcccc tcctcgcctc ggcccgctcc gccgcctgct ccagttccgg agtga
//
LOCUS       DASTXO010000208         2750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_822960, whole genome shotgun sequence.
ACCESSION   DASTXO010000208 DASTXO010000000
VERSION     DASTXO010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822960"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VFU96_04375"
     CDS             complement(<1..350)
                     /locus_tag="VFU96_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5112530.1"
                     /translation="MPMKITYATMSTDNEEMNRAYEDALESVKPRLGQVHGVAVDGDH
                     RTDRELYQEVSPIDSSIVVGRYAQAVAKDVDDAVSTAKAFAPEWEAWGWEGRRDLMLA
                     AADHMDSQLFELAAL"
     gene            467..1129
                     /locus_tag="VFU96_04380"
     CDS             467..1129
                     /locus_tag="VFU96_04380"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU5112531.1"
                     /translation="MPDSPWVLYWPLLLVGAYLLGSIPFAQVLARVRGVDLRYVGSGN
                     VGAGNLTRVAGWRWGVTAGVLDGLKGLFPVLIAQGMGFGKGAAGMVGLMAVIGHNWSV
                     FLKGRSGRGLATSVGLLLALDPVLLVWTTGWSVAGWKIGGGVAGFLGWGLLPIVSTAM
                     GRPATESLVLLALSIVIMARRMQGNPDSPRGMRPAMRRAIFDTDPGGGDFPQSADDLL
                     TP"
     gene            1126..2076
                     /locus_tag="VFU96_04385"
     CDS             1126..2076
                     /locus_tag="VFU96_04385"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEU5112532.1"
                     /translation="MSPKRVAWIAIIAAIVYAGFAGFILNNFELPEMSPYAWGLVALA
                     AVAQITASWFYGLLYKESVEEAGGELRAFSAFKAALVGAGVARLIPAGGAITPVAMGW
                     TVRDEEPGTTGAAVRAVLLNYAGLLMMTGVGLLLARPQDSARIASVSLTVLAPFVLLA
                     GVLLMFGSGKLGSLSRFLPKAVRKRLDTTVIDHLPGPESQTYIWCRLILEGTALGLVL
                     IAFGIDVNVFQVMAAFGAASIAGGLPGTPGGLGVTEAGLVFILSAYGFPAATTVAPVL
                     IYRIVSYWLPAALSFFAGGKTFLKSPEAKAATEVDYEGQR"
     gene            2091..2537
                     /gene="bcp"
                     /locus_tag="VFU96_04390"
     CDS             2091..2537
                     /gene="bcp"
                     /locus_tag="VFU96_04390"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEU5112533.1"
                     /translation="MPETVNFSLPNQDGDTVSSKDFKGKRLVMFFYPAAMTPGCTTES
                     CDFRDSYQEFGDAGYEIVGVSPDTPAKNARFKEKEGLNFDLLSDQDKELATSLGAWGE
                     KKMYGKTMEGLIRSTFVIGPDGKIEKAYRNVKATGHVARVKADLLG"
     gene            complement(2515..>2750)
                     /locus_tag="VFU96_04395"
     CDS             complement(2515..>2750)
                     /locus_tag="VFU96_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEU5112534.1"
                     /translation="LQQKDLRIVLDSADELGIPLPGTSLVFQLYRALQAHDLGSEGNH
                     ALVKALEELAGIELGGSRDRTSDDAGVSPGGLP"
BASE COUNT          434 a          871 c          911 g          534 t
ORIGIN      
        1 agcgcagcca gctcgaagag ctggctgtcc atgtggtccg ccgccgccag catcaggtcc
       61 cgtcggccct cccaacccca ggcctcccac tccggtgcga aagccttggc ggtggacacc
      121 gcatcgtcca cgtccttggc caccgcttgg gcatagcgcc cgacgacgat gctggagtcg
      181 atcggtgaga cctcttggta gagctctctg tcggtgcgat ggtcaccatc gaccgcaacg
      241 ccgtggacct gcccgaggcg cggcttgaca ctctccagag catcctcata ggcccgattc
      301 atctcctcgt tgtcggtgga catcgtggcg taggtgatct tcatcggcat ggctgtcctc
      361 ggtgcaggct catatcgacg gacgttaacc aacaaccgcc cccggaatcc tgggaacggg
      421 cccggctacc gaagcgagcg gaggctggct agcgtttctg gcgcagatgc cagattcacc
      481 gtgggttctc tactggcctc tcctgctggt gggggcctat ctcttgggtt cgataccgtt
      541 cgcccaggtg ctcgcccgcg tgcgtggtgt cgatttgcgc tatgtcggca gcggcaacgt
      601 cggcgccggc aatctgactc gtgtcgctgg ttggcgctgg ggtgtcaccg ctggagtgct
      661 cgacggcctc aagggtttgt tcccggtctt gatcgctcaa ggcatgggat tcggcaaggg
      721 cgccgccggg atggttggcc tcatggctgt catcggtcac aactggtcgg tgttcttgaa
      781 gggccgatcg ggacgtggct tggccacttc agtgggtctg ttgctcgccc tcgacccggt
      841 cctgctggtg tggaccaccg gttggtcggt ggccggctgg aagatcggcg gcggtgttgc
      901 aggcttcctg ggttggggcc tactgcccat agtctccacc gccatgggtc gtccggcgac
      961 cgagtcgctg gtgctgctgg cgctctccat cgtcatcatg gcccgccgca tgcagggcaa
     1021 tcccgacagc ccccgcggga tgcgtccggc gatgcggcgt gcgatcttcg acaccgaccc
     1081 gggtggtggg gacttcccac agagcgcgga cgacctgctg actccatgag cccgaagcgg
     1141 gtggcttgga tcgcgatcat cgccgcgatc gtctacgccg gcttcgccgg gttcatcctc
     1201 aacaacttcg agctgcctga gatgagcccg tacgcttggg gtctcgtcgc ccttgccgcc
     1261 gtcgcccaga tcacggcgag ctggttctac gggttgctct acaaggaatc ggtggaggag
     1321 gccggcggtg agctcagggc cttctctgca ttcaaggcgg ccttggtcgg tgccggtgtg
     1381 gcccggctca ttcctgccgg tggcgccatc accccggttg cgatgggctg gaccgtccgc
     1441 gacgaagagc ctgggaccac gggagcagcg gttcgagcgg tccttctcaa ctacgccggc
     1501 ctgctgatga tgactggagt cggcctgctc ctggcgcggc ctcaggattc agcccggatc
     1561 gcctcggtca gcttgacagt cctcgcccca ttcgtgctcc tggccggggt gttgctcatg
     1621 ttcggctcgg gcaagctggg ctcgttgtcg aggtttctcc cgaaggccgt gcgcaaacgc
     1681 ctcgacacca cggtcatcga ccatctgccc ggccccgagt cgcagaccta catctggtgt
     1741 cgcctcatcc tcgaagggac ggcgctgggc ctcgtgctga ttgccttcgg gatcgacgtc
     1801 aatgtgtttc aggtcatggc cgccttcggc gccgcgtcga tcgccggagg cctcccgggc
     1861 acccccggcg ggctcggcgt caccgaggcc gggctggtgt tcatcctcag cgcctacggg
     1921 ttcccggcgg cgacgacggt ggcaccggtg ctgatttatc ggatcgtctc ctactggctg
     1981 ccggccgccc tcagcttctt tgccgggggc aagacgttcc tcaaatcccc ggaggcgaag
     2041 gcagccaccg aggtggatta cgagggccag aggtgaagta gcctcgggca atgcccgaga
     2101 cagtcaactt cagtcttccc aaccaggacg gggacacggt cagctccaaa gatttcaaag
     2161 ggaagcgcct ggtcatgttc ttctacccgg ccgcgatgac accgggctgc accacggagt
     2221 cgtgtgactt ccgggacagc tatcaggagt tcggggatgc gggttacgag atagtcggcg
     2281 tgagcccgga cacgccggca aagaacgcgc ggttcaagga gaaggaaggg ctcaatttcg
     2341 acctgctctc cgatcaggac aaagagctcg ccacctcgct cggagcatgg ggtgagaaga
     2401 agatgtatgg aaagacgatg gagggcctga tcaggtcgac cttcgtcatc ggacccgacg
     2461 gcaagatcga aaaggcatac cggaacgtga aggccaccgg ccacgtcgcc cgggtcaagg
     2521 cagacctcct gggctgaccc ctgcgtcgtc cgatgtccgg tcacgcgagc cgcccaactc
     2581 gatgccggcc aactcctcca gggccttgac cagggcgtgg ttgccttcgg agccgagatc
     2641 atgggcctgg agggcccggt atagctgaaa gacgagcgac gtcccgggca ggggaatccc
     2701 aagctcgtcg gcagagtcga gcacgatgcg gaggtccttt tgctgaaggt
//
LOCUS       DASTXO010000209         4631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1265120, whole genome shotgun sequence.
ACCESSION   DASTXO010000209 DASTXO010000000
VERSION     DASTXO010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265120"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VFU96_04400"
     CDS             complement(<1..190)
                     /locus_tag="VFU96_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HEU5112535.1"
                     /translation="MSLPDTIRERRVIAVARGVTPENAPALARALLDGGIGVLEVTVE
                     EEQGIPALSSIVGAEMLVG"
     gene            complement(201..974)
                     /locus_tag="VFU96_04405"
     CDS             complement(201..974)
                     /locus_tag="VFU96_04405"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5112536.1"
                     /translation="MRLADHVVVVTGATGIAAAGARRFVSEGASVFIVSIDEDQCRHL
                     AAELEGAGPIGWSAADLTDEAATEAAFAACLERFGAVTGLYAVVGASGRGKGDGPLDQ
                     IPLEGWRHTMDINTVPPFLAAREAVKSMKRRDAGGSVVLIGSISAMHPSQYFVTHAYA
                     ASKGAAISLTRSLASHYASDRIRVNLIAPGLVDTPMSIRAASDPPTVAYAREKQPLAG
                     GFLDPSDIAAAAVFLLSDESAQITGQVLEVDGGWGVTEA"
     gene            1095..1634
                     /locus_tag="VFU96_04410"
     CDS             1095..1634
                     /locus_tag="VFU96_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112537.1"
                     /translation="MTAIDRDKLVAAEETSRPRKPFVLLTAAILSLAVGLGSLAGGIF
                     GAWYTWDQAVAQDVTTPDDAVIPETPVRGPFTMWAQADIITHHQLDNTEGLYYSQMPR
                     EVPMVDENGAAILDENGEQVMGPNEARASWIPATTLTTTLGLGIVSYALSAFAVVIWL
                     TLMFTGYVFLYIRKRAVLL"
     gene            complement(1698..2837)
                     /locus_tag="VFU96_04415"
     CDS             complement(1698..2837)
                     /locus_tag="VFU96_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112538.1"
                     /translation="MIATFVVIMLEISGAVALVALGGENPASQSTAYATVLFVLTTGA
                     VGTLIGLRRPGNTIGWLLLVAAFGFATGSLLVAYVDFAVAEAGSLPVGPLAVWLGDLM
                     FQVSLGVSATFLLLLFPTGHLPSPRWRVVGWLAAIGMALGFAAVLFGPGAFEGLPIEN
                     PLALDATSPMLRVFENGFYLILVPTLASVASLIVRYRGAVDEEKQQLKWVALGVIVLA
                     VGMAGSVVWSGLSDDTVNLVIALSLTAVPISIGMAILRYRLYDIDRIISRTVSYGVVT
                     VLLAGAYVGLVFVLGDLVPSQGNSLVVAASTLAVAALFNPLRRWVQDFVDRRFNRSRY
                     DLARTIEGFAERLRTEVDLEQLGRDLQFVAADTMNPTSMSLWLRD"
     gene            complement(3024..3668)
                     /locus_tag="VFU96_04420"
     CDS             complement(3024..3668)
                     /locus_tag="VFU96_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112539.1"
                     /translation="MDVFHVGEVAEGARKGNVEGRKPTGPLQGFGQLWQKTYEVRIPG
                     PTPEEVIATWKAKFGEFWPSYNKFYAPAGGIAPGEVAVIGGGKGPAKITTGVRVIYAD
                     ERSWSYMTPEGHPWAAIITFSAHEGEDGATVARIHLLVRANDPLYEVSFKLYTSRMED
                     QIWTHTLTQVAAHFEVVSPVVETSVLLVDKKRQWDQFSNIYKNSALRTMLRRDR"
     gene            3834..>4631
                     /locus_tag="VFU96_04425"
     CDS             3834..>4631
                     /locus_tag="VFU96_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5112540.1"
                     /translation="MVEYDVVVVGSGPNGLSAAAELSRRGRRVLVVEQADEIGGGTRT
                     EELTLPGFAHDICSAIHPLGVASPFFRDLGLEVDWVHPTIPLAHPLGGGRAGAILRDV
                     EATADRFGPDARHYKRILGPLVEAADAVMEDFLGPMTLVPDNPGSFVKLASRGALPAS
                     VIASGFSSDEARGVFAGLAAHAIAPFSSPLTGGVALLFGVAAHAYGWPMARGGSQRIA
                     EALAKIIVDGGGIIETGRMVGSLDDLPTSDAVVLDVMPRAAVRIGGNR"
BASE COUNT          872 a         1520 c         1436 g          803 t
ORIGIN      
        1 caccgacgag catctctgcg ccgacgatgc tcgacaaagc cgggatcccc tgttcctcct
       61 cgaccgtgac ctcgagaact ccgatccctc cgtccaacaa cgcacgggcg agagccggtg
      121 cgttttccgg cgtcacacca cgtgccactg cgatcactcg cctctcccgg atcgtatcgg
      181 gcagtgacac tcgtctcacc tcatgcctcg gtgacacccc atccaccgtc gacctccagc
      241 acctgcccgg tgatctgcgc cgactcgtcg gagagcaaga aaacggcggc tgcggcgatg
      301 tccgacggat ccaagaagcc ccctgccaac ggctgcttct cgcgggcgta ggcgacggtg
      361 ggcgggtccg aagccgcacg gatggacatt ggcgtgtcaa ccagccccgg agcgatcagg
      421 ttgaccctga ttcgatcgga tgcgtagtgc gacgccagtg acctcgtcag ggaaatggcc
      481 gcgcccttgg acgccgcgta ggcgtgggtc acgaagtatt gcgaggggtg catggcggag
      541 atgcttccga tcaggaccac cgatcctccg gcatcgcgac gcttcatcga cttgaccgcc
      601 tctcgagcgg cgaggaacgg agggacggtg ttgatgtcca tggtgtgacg ccacccctcg
      661 agtggaatct gatccaacgg cccgtcacct ttgccccgtc cgcttgcgcc cacgacggca
      721 tagagaccgg tcaccgcgcc gaagcgctcc aggcaggcgg cgaaagcggc ctcggtggcc
      781 gcttcgtcgg tgagatcggc cgccgaccag ccgatcggtc cggcgccctc cagctcggcc
      841 gcgaggtgac gacattgatc ctcgtctatc gagacgatga acaccgatgc gccttcagag
      901 acaaatcggc gggcgccggc ggcagcgatc ccggtggccc cggtcaccac gacgacgtgg
      961 tcggcgagtc gcatgcctgc gtttgtagca ggacttttgg tcccggtctt cgggaccaaa
     1021 gcccctcgtg caatctttca caagggccga ggatgaccct ataagcagtt ggtcctacaa
     1081 gaaggaggcg ccaaatgact gctatcgata gggacaagct ggtagctgcg gaagagacca
     1141 gccgtccacg caagcctttc gtcctgctca ccgccgccat cctgtcgctg gccgtcggcc
     1201 tcggctcgct ggctggtggc atcttcggtg cctggtacac ctgggaccag gcagtggccc
     1261 aggatgtgac cacacccgat gacgcggtga tcccggagac accggtgaga ggcccattca
     1321 cgatgtgggc acaagccgac atcatcacgc accaccagct cgacaacacc gagggtctct
     1381 actactccca gatgccccgg gaagtcccga tggtggatga gaacggcgcg gccatcctcg
     1441 acgagaacgg cgagcaggtc atggggccca acgaggccag ggcatcctgg atccccgcca
     1501 ccaccctgac cacgactctg gggctgggaa tcgtctccta cgcactgtcc gccttcgcgg
     1561 tagtgatctg gttgaccttg atgttcaccg gctacgtgtt cctctacatc cgcaagaggg
     1621 cagtcctcct gtaggaacca acgctcagcg gatagggatc aggcccccgg caacgggggc
     1681 ctgatccgcg tgccaactca gtcgcgcaac caaagcgaca tcgacgtcgg gttcatggtg
     1741 tcggcggcga caaactgcaa gtctcgtccg agctgttcca gatccacctc tgtgcgcaga
     1801 cgctccgcga agccctcgat ggtgcgagcg aggtcgtatc gagaacggtt gaagcggcgg
     1861 tcgacgaagt cctggaccca acggcgcagc gggttgaaga gagctgcgac cgccaatgtc
     1921 gaggcggcca cgacgagact gttcccttgt gaaggaacca ggtcaccgag cacgaagacc
     1981 agcccgacgt aggctcctgc caagagcacg gtcacgacac cgtatgacac cgtgcgcgag
     2041 atgatgcggt cgatgtcata gagccggtat cggaggatcg ccatcccaat ggagataggt
     2101 acggccgtta gtgacaacgc gatcacgaga ttcacggtgt cgtcgctcag accgctccaa
     2161 accacagaac cagccatacc gaccgccagc acgatcacgc ccagggcgac ccacttcaac
     2221 tgttgtttct cttcgtccac cgccccgcga tatctgacga tcagcgaggc caccgaggcc
     2281 agggtcggaa ccaggatcaa gtagaacccg ttctcgaaca cccgcagcat cgggctcgtg
     2341 gcgtcgaggg cgagcgggtt ctcgatagga aggccctcga aggcgcccgg gccgaacaag
     2401 acggccgcga aaccaagtgc catgccgatc gccgccaacc agcccacgac cctccagcgc
     2461 ggcgagggga ggtgaccggt cgggaagagc agcaacaaga atgtcgcaga caccccaagg
     2521 ctcacctgaa acatgagatc gccgagccag accgcgagcg gcccgactgg caacgagccc
     2581 gcttcggcta ctgcaaaatc gacgtaggcg accagcaatg aaccggtggc gaagccaaaa
     2641 gcggcgacca agagaagcca cccgatcgta ttgccgggtc ggcgcagccc gatcagggtt
     2701 cccacggcgc cggtggtcag cacgaacagc accgtggcgt aggcggtgga ctgggatgcc
     2761 gggttttctc ccccgagagc gaccaaggcc accgcaccgc ttatctcgag catgatcacg
     2821 acgaaggtcg cgatcacgag ccacgaactt gatcgtctca tggtcgcgcc ggctcgattt
     2881 cgacagcggt gtgctcgacc cccctcaggt ctggcatggc caagacgacc aggatcatgg
     2941 gtgggacgat attgccgatc acaactccac gccgcaggcc gtcgaacggt tcggttcagc
     3001 cctggacgtt cacacttttg cttctaccga tcacggcgga gcatggtccg cagggcggag
     3061 ttcttgtaga tgttggagaa ctggtcccac tgtcgcttct tgtccacgag cagcacactg
     3121 gtctcgacca ccgggctcac cacctcgaag tgggctgcga cctgggtcaa ggtgtgagtc
     3181 cagatctgat cctccatccg ggatgtgtag agcttgaagc tcacctcgta caacgggtcg
     3241 ttggcccgga ccaggagatg gatcctggca acggtcgccc cgtcctcgcc ctcgtgagcg
     3301 ctgaacgtga tgatggccgc ccatgggtga ccttcgggag tcatgtagga ccatgagcgt
     3361 tcgtcggcgt agatcaccct gactccggtg gtgatcttcg ccgggccttt gcccccgccg
     3421 atcacggcca cctcgccagg ggcaatgcca ccggcgggcg catagaactt gttgtaggac
     3481 ggccagaact cgccgaactt cgccttccag gtggcgatca cctcttccgg ggtcggcccg
     3541 gggatacgca cctcataggt cttctgccaa agctggccga agccctgcag cggcccggtg
     3601 ggtttgcgac cctccacgtt gcccttgcgc gcaccctcgg cgacttcccc aacatggaat
     3661 acgtccaccg gctgtgccca attctcagtg tccctcggct cggtcaactc ggtcctttct
     3721 ccgattccag caatagacca caagggcgtt ccgccatcgt tacgggggta ttcgtcccgg
     3781 ttccagtctc cggtgaccat gtcgtaacgc ccctcgggca gaatcagggg gacatggtcg
     3841 aatatgacgt agtcgtggtc ggctccggcc ccaacgggct ctcggccgcg gcggagctgt
     3901 cacgacgagg gcgacgggtg ctggttgtgg agcaggccga cgagatcggt ggtgggaccc
     3961 ggaccgaaga gctcaccctt cccggcttcg cccatgacat ctgttctgcg attcatcccc
     4021 tcggtgtcgc ctcgccgttc tttcgtgacc tgggcctcga agtggattgg gtccatccca
     4081 cgatccccct cgcccatccg ctcggcggtg gccgcgccgg tgcaattctg agagatgtcg
     4141 aggccacagc cgatcgattt ggccccgacg cacgtcacta caagagaatc cttggcccgc
     4201 tggtcgaggc tgcggatgcc gtaatggagg acttcctggg gcccatgacg ttggtccccg
     4261 acaatccggg gagcttcgtc aagctggcca gccggggcgc gttgccggca tcggtcattg
     4321 cctcggggtt ctcctccgac gaggcacgag gcgttttcgc cggtctcgcc gcccatgcga
     4381 tagccccgtt cagctcaccg ctgaccggtg gggtggcgct gctattcgga gttgccgcac
     4441 acgcttatgg ctggccgatg gcccgtggtg gatcacaaag gatcgccgag gctctggcca
     4501 agatcatcgt cgacgggggt ggcatcatcg agaccggccg catggtcggc tcgctggacg
     4561 acctcccgac cagcgacgcg gtcgtgctgg atgtgatgcc acgagcagcg gttcgaatcg
     4621 gcggcaaccg t
//
LOCUS       DASTXO010000210         2900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1670435, whole genome shotgun sequence.
ACCESSION   DASTXO010000210 DASTXO010000000
VERSION     DASTXO010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1670435"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VFU96_04430"
     CDS             <1..232
                     /locus_tag="VFU96_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112541.1"
                     /translation="ATHSWMCERSSGSRAPCIVRLSVVGIAEACIPDPDDRLHDLLAT
                     VIDDCAGRLGPAQVVDTAQASAAMFRAPAVFR"
     gene            complement(243..1223)
                     /locus_tag="VFU96_04435"
     CDS             complement(243..1223)
                     /locus_tag="VFU96_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112542.1"
                     /translation="MSRTDAATTRVPGETSTTSTSTTQPARTELRGETPVEIGTPIEP
                     AELTGRVVFDDFEDLFTSSISGSHLQQITAQPGSEFDAAWSPDGQRVVYRDSRRGINN
                     DDEIYVASADGSDARNLSANPANDWGPDWSSDGEWIVFNSDRDGSPLSGYLVRPDGSD
                     LRRIDTEVWVEYPSFSPDGTRIVFEGHSSGDYDIYVGDLATGETRQLTDSPGGDGWPV
                     WSPDGEWIAFTTERDDCLRAPPDQDCWTSGEPGEHRSVWLMRPDGSEQHRVTPESGQF
                     VAWSPNAQYLLISGRTLYVLRPDGTGRWEFWPEGLDLPPGGIPDWTAAER"
     gene            complement(1393..1776)
                     /locus_tag="VFU96_04440"
     CDS             complement(1393..1776)
                     /locus_tag="VFU96_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013416643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5112543.1"
                     /translation="MSGRVVHFEIPYDDGQRARDFYAKAFGWEVTEMPEMNYTMVSSG
                     PSGDQGPTEAGFINGGMMQRQEPFMAPNLVIDVESIDAALAVISEAGGSPVSEREAVG
                     EMGFAAYFKDTEGNLVGLWESVPQG"
     gene            1888..2388
                     /locus_tag="VFU96_04445"
     CDS             1888..2388
                     /locus_tag="VFU96_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112544.1"
                     /translation="MDGDSRPSPEVGSELGEVERRVVDRMQHPDEAQAREWLDQNALT
                     YGALIGVAVLMVQPFLTATSLDLAATISVVAFSVAIPLLAALILVSRQETFHGRRTRS
                     TLVTISQAVAQGTAIIGLVAAFWHIHWIAGVGLLVSGFLAIAIHSAGFWRLELDVASS
                     PDEPQS"
     gene            complement(2441..>2900)
                     /locus_tag="VFU96_04450"
     CDS             complement(2441..>2900)
                     /locus_tag="VFU96_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112545.1"
                     /translation="ALPGRGRAPMTDRDQTQKMIDALVERARALSDDDRIRLAAARAG
                     IDESFHAGAWRAASEMLPVRAEMYASAWQQIGPAFVPDQLLEIVEMGDRADPEELLEW
                     QEVARLVRLGIDDELLALLTSDLIPPPHLRQLHRSWAAMVDQSHPPEDGP"
BASE COUNT          482 a          963 c          902 g          553 t
ORIGIN      
        1 agcgacacac tcctggatgt gcgaacgctc aagcggcagt cgggccccct gcatcgtccg
       61 tttgtcagtc gtcggcatcg ccgaggcttg catccccgat cccgacgacc gtcttcatga
      121 cctgctcgcg accgtgatcg acgactgcgc cggccggttg ggcccggccc aggtcgtcga
      181 cacggcccag gcgtcggcgg cgatgttccg cgccccggcg gtgtttcgct agttcgacac
      241 catcagcgtt ctgccgccgt ccagtcggga atgccacccg gcggcaggtc caggccttcc
      301 ggccagaact cccagcgtcc cgtgccgtcg ggccgcaaca cgtacaacgt ccggcctgaa
      361 atcaagaggt attgggcgtt cggcgaccat gcgacgaatt ggccggactc cggcgtgacc
      421 cgatgttgct ccgagccatc gggtcgcatg agccacacgc tgcggtgctc accgggctct
      481 ccactggtcc aacaatcctg atcgggagga gctcgaagac agtcatcgcg ctctgtggtg
      541 aatgcgatcc actcgccatc gggcgaccag accggccagc cgtcgccgcc cggtgaatcg
      601 gtcaactgtc gtgtctctcc tgtcgccagg tcaccgacat agatgtcgta gtcgccgctc
      661 gagtggcctt cgaagacgat cctggtcccg tccggggaga aggatgggta ctcgacccag
      721 acttcggtat cgatccgacg taggtcgctc ccatcgggcc gcaccaggta gccactcagc
      781 ggtgacccgt cgcggtcgga attgaacacg atccattccc catcggatga ccagtcaggc
      841 ccccaatcgt tggccgggtt cgcagacaag ttccgagcat cggatccatc agccgaagcg
      901 acatagatct cgtcgtcgtt gttgatgccg cgtcgcgagt cgcggtacac cacccgttgc
      961 ccgtctggtg accatgccgc atcgaattcg gagcccggct gggccgtgat ctgctgcaag
     1021 tgggagccgc tgatcgagga cgtgaacaag tcctcgaaat catcgaacac cacgcgaccg
     1081 gtcagctccg ccggctcgat cggcgtcccg atctcgacag gtgtctcacc gcgaagttcg
     1141 gttcgggccg gctgggtcgt cgatgtgctc gtcgtcgatg tctctccagg cactcgggtc
     1201 gtcgcggcgt cggtgcgaga catctcggag gtccggcagg cggaaaccac cagtagcccg
     1261 ataagggtca acgcccacca cggagtcaac cggctgctgg gtgggtgtcc acggccgatc
     1321 acgtcagcta tacaacccaa acaggcccgt ggttccagct cagtttttct agacgggtgc
     1381 cgtgagccgg cttcagccct gaggcacgct ctcccagagt ccgaccagat tgccctcggt
     1441 gtctttgaaa taggcagcga agcccatttc tccgaccgct tcgcgttcac tgacggggga
     1501 tccgccggct tcgctgatca ccgccagggc agcgtcgatg ctttccacat cgatcacgag
     1561 gttgggggcc atgaaaggct cctgacgctg catcatgcca ccattgatga acccggcctc
     1621 tgtcggcccc tggtctccac tcggcccgga tgaaaccatg gtgtagttca tctcgggcat
     1681 ctcggtgacc tcccagccga atgcctttgc atagaagtca cgggctcgct ggccgtcgtc
     1741 gtagggaatc tcgaagtgca cgactcgacc ggacattggg agtcctcctt cgtctcgatg
     1801 acatcgccga ccatagctcg ggcgaacatc tctcggccgg gcctgggccg cggtttgccc
     1861 tccagagcac ctgggtagag ccaccggatg gatggcgatt cccgcccctc acccgaggtc
     1921 ggaagtgagc tcggtgaagt cgagcggcgg gtcgttgatc ggatgcagca tcctgacgag
     1981 gcccaagcga gggaatggct ggatcagaat gccctgacct atggcgcgct gatcggcgtc
     2041 gcggtgctga tggtgcagcc gttcttgacg gccacatctc tcgaccttgc cgccacgatc
     2101 agcgtcgttg ccttttccgt cgccatccct ctccttgccg cgctgattct ggtcagtagg
     2161 caggagacct ttcatggtcg gcgtacccgg tccactcttg tcacgatcag ccaggcggtg
     2221 gcgcagggga cggcaatcat cgggctggtc gctgccttct ggcacatcca ttggatcgct
     2281 ggagtcggac tgctcgtcag cggcttcctc gccatagcga tccactcggc gggcttctgg
     2341 cggctggaac tggatgtggc ctcctcaccc gacgagcccc aaagctgatt cttgcctgca
     2401 tgcgggcgtg gttctgctcg gcgacaaacc gggtgccggg ttacgggccg tcctccggtg
     2461 gatgggactg atcaaccatc gcggcccacg atctgtggag ctgacggagg tgggggggtg
     2521 gtatcaagtc ggaggtgagg agggccaaga gctcgtcgtc gatcccgaga cgcacgagac
     2581 gggcgacctc ttgccactcc aggagctctt ccgggtcggc tcggtccccc atctcgacta
     2641 tctccagcaa ttggtccggc acgaacgccg ggccgatctg ctgccaggcc gaggcataca
     2701 tctctgcccg gaccgggagc atctcggacg ccgcccgcca cgcgcccgca tggaaggact
     2761 catcgatccc agcccgagcc gcggcgagcc ggattcgatc gtcgtccgac agcgcccggg
     2821 cccgttccac cagggcgtcg atcatcttct gagtctggtc tcgatcggtc attggtgccc
     2881 tcccgcgccc aggcagggca
//
LOCUS       DASTXO010000211         2659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1633754, whole genome shotgun sequence.
ACCESSION   DASTXO010000211 DASTXO010000000
VERSION     DASTXO010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633754"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1627
                     /locus_tag="VFU96_04455"
     CDS             <1..1627
                     /locus_tag="VFU96_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5112546.1"
                     /translation="EALQAIDREARAVRGVRLVVLVTGTGGLARVNQGNGYVRIAPHA
                     ERTVTLTRLWEGARRGDPLAAFRDNYSQRQVMTELRQRLRKFTDMRISIRNIPGFNIG
                     GGNFDIDFVLRGPDLETLARYGDQLRRRGIELGGMNDLNTTLRLDRPELRVQIDRERA
                     ADLLVDTEQIATTLRLMVGGDQEVSRFRDPVINEDYDVQLRLREEDRRDPRTISRLFV
                     SRPGATAGTGVGAAPALGLAPGGGLVRLDSLVKIVPTRTAPRIDRSDRQREVRLRASV
                     APGYGLADRIDALRQAAAQRDLQPGYTTAVSGRASEFEKTFKEFLWVFLLAVIFMYMI
                     LASQFESLIHPLTILLSLPLAVPFALFSLWYTGNTLNLYSALGILVLFGIVKKNSILQ
                     IDQMNALREAGHDRAYAIIEGNRNRLRPILMTTLALVAGMLPLWLGTGPGAEERRAIA
                     VVVIGGQSLCLLLTLLVTPVAYSLFEDFARAMGWQGLSRRSRRMMDRLRPENGWWRPR
                     TANGSALVPRVPVAPFEDDDREASAPKPEAGRR"
     gene            1655..>2659
                     /locus_tag="VFU96_04460"
     CDS             1655..>2659
                     /locus_tag="VFU96_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEU5112547.1"
                     /translation="MYPHQNERLTVALDRAGLEALVATSAANVAYVAGFVGVADPGAT
                     SSGSAIFTRQGTALVLPAIAAPSAVIEGLEVNHLVCFGDFHAAFQHPRDASLSRLQSV
                     LDQRAASLPEALGLAFGLLGVRSGTVGVDMGGLAPLEWERVNEWLVPLKIVSGADHLA
                     AARRVKAPFEIECLQRALGIAEEALNEVIQTIARGTTEREAASAYRAEVVKRGADPCR
                     VLVLMGERTALPLARPTDRALRPGELVRFDVGCVYRGYHAGVARTGVLGAPTSEHDAS
                     SGALLAGMEAAIAGVEPGRSAGHVPTATVQAVRTHGLGRYRNDSVGHGIGLEPCESPS
                     L"
BASE COUNT          402 a          886 c          887 g          484 t
ORIGIN      
        1 cgaggccctg caggccatcg atcgagaggc gcgcgcggtg cgtggagtgc ggctggtcgt
       61 cctggtgacc gggacgggag gactggctcg cgtcaatcag ggcaatggct atgtccgtat
      121 cgcaccacat gccgagcgca cggtgaccct gactcgtctg tgggagggag cgcgtcgagg
      181 cgatccgctc gccgcgttcc gagacaacta ttctcagcgc caggtcatga cggagctgcg
      241 ccagcgcttg cgcaagttca ccgatatgcg gatctcaatc cgcaacatcc cgggtttcaa
      301 catcggaggc ggtaacttcg acattgactt tgtcttgcga gggcccgacc tggagacgct
      361 ggctcgctac ggtgatcagc tgcggcgcag agggatcgag cttggcggca tgaacgacct
      421 caacaccacc ctccgtctcg atcggcctga actccgggtg cagatcgatc gggagcgggc
      481 ggccgacctg ctcgtcgaca ccgagcagat cgcgaccacc ctgcgtctca tggtcggtgg
      541 agaccaggaa gtctcgcgct tccgcgatcc cgtcatcaac gaggactatg acgtccagct
      601 tcgcctgcgc gaggaggacc ggcgcgaccc gagaaccatc tcacgcctgt tcgtgtcgcg
      661 cccgggagcc accgctggca ccggggtggg tgcagcgccc gccctcgggc tagcaccggg
      721 gggcggattg gtccggctcg acagcctcgt aaagatcgtg cccacccgga cggctccccg
      781 catcgatcgg tccgatcgtc agcgcgaggt gaggttgcgg gcctcggtgg cgcccgggta
      841 tggcctcgcc gatcggatcg atgccctccg ccaggcggcc gcccagaggg atctccagcc
      901 cggctatacc acggccgtgt cagggcgggc gagcgagttc gagaagacct ttaaggagtt
      961 cctgtgggtg tttcttctcg cggtgatttt catgtacatg atcctggcct cgcagttcga
     1021 gagcctcatc cacccgctga cgatcctgct ctcactccct ctcgccgtgc cgttcgcgct
     1081 cttctccttg tggtacacgg gcaataccct gaatctgtac tcggcgctcg ggatcctggt
     1141 tctgttcggc atcgtgaaga agaactcgat cttgcagatc gaccagatga acgccctgcg
     1201 cgaagccggt cacgatcggg cctacgcgat catcgagggt aaccgcaacc gactccgccc
     1261 gatcctcatg accactctgg ctctggtggc gggtatgctg ccgctgtggc tcggtactgg
     1321 tcccggcgcc gaggagcgtc gcgccattgc tgtcgttgtc atcggcggtc agtcgctgtg
     1381 cctgttgctc actctgctgg tcacgccggt ggcctattcg ctcttcgagg actttgcgcg
     1441 agccatgggc tggcagggtc tctcacggcg aagtcggcgc atgatggacc ggctccggcc
     1501 agaaaacggg tggtggcggc cccgcaccgc caatggctcg gccctcgttc cccgggtccc
     1561 ggtcgccccc ttcgaggacg acgaccgcga agcctcggcg ccgaagcccg aagccggacg
     1621 ccgctaaagc ttccaagcca tcgagggcag atacatgtac ccacatcaga acgaacgtct
     1681 gaccgtggcc ctggaccggg ctggcctgga ggccctggtg gccacgtcgg ccgccaacgt
     1741 cgcctatgtg gccgggttcg tcggtgtcgc cgaccccgga gccacttcga gcggatcggc
     1801 catcttcacc aggcaaggga cggccctggt gctcccggcc atcgcggcgc cctcggccgt
     1861 catcgagggg ctcgaggtga accacctcgt gtgcttcggt gattttcacg cggcattcca
     1921 gcatcctcgg gatgcctccc tgtctcgctt gcagtcggtc ctggaccagc gggcggcgag
     1981 tctccccgag gcgctcggac tagccttcgg tctgttgggt gtacgcagtg ggacggtcgg
     2041 cgtggacatg ggcgggcttg cccccctcga gtgggaacgc gtgaacgaat ggctcgttcc
     2101 cctcaagatc gtaagcggtg ccgaccacct cgcggccgcg cgccgtgtga aggcaccgtt
     2161 cgagatcgaa tgcctgcaac gcgctctggg cattgccgag gaggcgctca acgaggtgat
     2221 tcagaccatc gctcgcggga ccaccgagcg cgaggcggcc agcgcgtatc gggcggaagt
     2281 cgtgaagcgt ggcgccgatc cgtgccgcgt tctggtcctg atgggcgagc gtacggccct
     2341 tcccctggcc cggccgactg accgggcatt gcgtccgggc gagctcgtgc gtttcgatgt
     2401 cggatgcgtc tatcgcgggt accatgcggg tgtcgcccgg acgggagtgc tcggcgcgcc
     2461 cactagcgag cacgacgctt cctctggagc cctcctggcc ggtatggagg ccgccatcgc
     2521 cggcgtggaa cccgggcgct cggcgggcca cgttccgacg gcaacggtcc aggccgtgcg
     2581 tactcacggt ctggggcgtt accgaaacga ttctgtcggc cacgggatcg gcctcgagcc
     2641 ctgcgagtcc ccgtccctc
//
LOCUS       DASTXO010000212         2745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_934287, whole genome shotgun sequence.
ACCESSION   DASTXO010000212 DASTXO010000000
VERSION     DASTXO010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934287"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..743
                     /locus_tag="VFU96_04465"
     CDS             99..743
                     /locus_tag="VFU96_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HEU5112548.1"
                     /translation="MTAVDLPKAILFDAGGTLLLQDTEEMGHLLGVPIDAAGAHRAHY
                     QAMAEYSDRRLAGQDVDWDWWMERYFTILGVVDPHLAGEKTDHGYGLWNHALDGVAQA
                     VQRLRDSGVRVAVVSNSDGSVRGSLEKAGLLDLFEFVVDSHEVGVAKPDPGIFHAALE
                     HMGLDPSDAWYIGDSVFHDVNGAQAAGLSRALLVDPYGLGPANVKSIGSVAELA"
     gene            complement(714..1232)
                     /locus_tag="VFU96_04470"
     CDS             complement(714..1232)
                     /locus_tag="VFU96_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020475160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5112549.1"
                     /translation="MDVNPVPDAYGSVTVSLTVSPCDEAIEFYKSAFGAEEATPPMIG
                     PDGKVSHAEIRIGDTIVMLSDEQPDSPTKSPRSAGTTTAVLFLYTEDVDSLWERAIAL
                     GAREVYPLSLQFYGDKGGRVEDPFGHQWGLAQHVEDVSPDEMERRIAEFYEASREERQ
                     RLLSRELGDRPD"
     gene            complement(1306..1857)
                     /locus_tag="VFU96_04475"
     CDS             complement(1306..1857)
                     /locus_tag="VFU96_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112550.1"
                     /translation="MTGAADFRGRLSGWLLIGSGAVYFLFYGLQFLGVDLDGPQELAI
                     FVHLILILLTLAGLIGARRALSLESWLGALWTMSTVLAVLGLTVGKPFWSAAMFGLGI
                     VSMALWRAHVVASLLVIGGAIWLYLFAAGVRVGGENGRPMLGIEPDLALVAVVLMALG
                     LVGLGRKVRGATSHLTAARQASR"
     gene            2017..2409
                     /locus_tag="VFU96_04480"
                     /pseudo
     CDS             2017..2409
                     /locus_tag="VFU96_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004546866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
     gene            2461..>2745
                     /locus_tag="VFU96_04485"
     CDS             2461..>2745
                     /locus_tag="VFU96_04485"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5112551.1"
                     /translation="MSTATAPNGTVTILFTDIEGSTQLTEQLGDEDWLTVLRAHNKVV
                     RDQVKDHAGFEVKSQGDGFMLAFPSAKEALRCAIGIQRALATREPNGVVLR"
BASE COUNT          533 a          876 c          854 g          482 t
ORIGIN      
        1 gcgatctcga gatcgctcgc tatcggtggc agcaggttcg acatgccgta gttctagcgg
       61 gttcgggtct agcgggcctg atctgctgat atgccgtcgt gacagctgtg gatttgccca
      121 aagcgatcct tttcgatgcc ggtggcactc tcttgctcca ggacaccgaa gagatgggac
      181 accttctggg tgtgccgatc gacgcggcag gggcccaccg cgctcactac caggcgatgg
      241 ccgaatattc agatcgccga ctcgcaggtc aggacgtcga ctgggattgg tggatggagc
      301 gctacttcac gattctgggc gtcgtagatc cgcatctggc cggggagaag actgatcacg
      361 gctacgggct gtggaaccac gccctggatg gcgtggccca ggccgtccaa cggctccgtg
      421 actccggtgt cagggtcgcc gtggtgtcga attcggacgg ctcggtgcgt gggtccctgg
      481 aaaaggcggg tctcctcgac ctgttcgaat tcgtcgtcga ctcacatgaa gtcggggtgg
      541 ccaaacccga cccggggatc ttccatgctg ccctcgaaca catgggcctg gatccgtcgg
      601 acgcctggta catcggcgac tcggtgttcc acgatgtaaa cggagcccag gcggcagggc
      661 tgtcacgagc gttgttggtc gatccctacg gcctgggccc ggcgaatgtc aagtcaatcg
      721 ggtcggtcgc cgagctcgcg tgagagaagg cgctggcgtt cctccctcga agcctcgtag
      781 aactcggcga tcctccgctc catctcgtcg gggctgacgt cttccacgtg ctgcgccaac
      841 ccccattggt gcccgaaggg gtcttcgaca cgcccaccct tgtcaccata gaactgcaga
      901 gacaatgggt agacctcgcg ggctcccagg gcgattgccc gctcccagag agaatcgaca
      961 tcctctgtgt agagaaagag gaccgccgtg gtggtcccgg ccgaccgcgg cgatttggtg
     1021 ggtgagtcag gctgctcgtc gctcagcatc acgatcgtgt cgccgatccg gatctcggcg
     1081 tgggagacct tcccgtcggg tccgatcatc ggcggcgtcg cctcttcggc cccgaacgcc
     1141 gacttgtaga actcgatcgc ttcgtcgcaa gggctcacag tcagagaaac cgtcacagag
     1201 ccataggcat cgggcacagg gttcacgtcc acggtcgacc ctcctctgga tgtcctcacc
     1261 ctacgccggg agtcccccac ggcctcggcc cgctcgacgt cggttctatc ggctcgcttg
     1321 ccgagccgcg gtgaggtgac tcgtggcgcc acgaaccttc cgcccgagtc cgacgagacc
     1381 aagggccatc aggacgaccg cgaccagggc gagatctggc tcgatgccca gcatgggtcg
     1441 accgttctcg ccaccaacgc gcacccccgc tgcgaacagg tagagccaga tcgcaccacc
     1501 gatgacaagc agcgaagcga cgacatgagc tcgccacaag gccatggaca cgatgccgag
     1561 gccgaacatc gctgccgacc agaacggctt gccgacggtg aggccgagca cggcgagaac
     1621 cgtcgacatc gtccatagcg ctccaagcca gctctcgaga ctgagtgcac ggcgggctcc
     1681 gatcagaccg gcaagtgtca acaggatcag gatgagatgc acgaaaatcg cgagttcctg
     1741 cggaccgtcg aggtctactc ccaggaactg gagtccatag aacaagaaat agacagcacc
     1801 gctgccgatc agcagccagc cgctgagcct cccccgaaag tcggcagcgc ccgtcatcgg
     1861 tgaaagctca ttccacgaaa acgttggtga cgcactgttc cggtacctct acgacattga
     1921 tgtccagcac agaaggtgtg cttaccgcga gatcggcttg gtctttctca ttagcgcact
     1981 cgaccggtcg gaggattccc ctggtaggaa tacgggatgc gctacatcat cctcgtcaag
     2041 gcctcagcag ccaccgaagg cggggagatg ccgagtcagg agttgatggg cgaggtggcc
     2101 agatacaccg aggaactggc cagggccggg gccctctacg acgccacagg cctaaagccc
     2161 acatcggccg ggtggcgcat caggttccgc ggggaggatc gtgagatggt tcccggaccg
     2221 ttcccggact cccccgacat catcgccggc tacaccatca tccgagtcga cagccgagag
     2281 gaggccatcg agtggtcgat gcgttatcca aatcccgccg gcgtgctcgc cgccgccgag
     2341 atcgaggtgc gcgaattctt cgagctcgaa gacttcgata agggcccggc gatcgatcac
     2401 ttccgtgagc tgggcctgat cgacaccatc gccactgcga tcatccggtc ccgtccggac
     2461 ctgtcgacgg cgaccgcccc caacggaaca gtcaccattc tcttcaccga catcgagggc
     2521 tcgacccagc tcacagaaca gttgggagac gaggactggc tcacggtgct tcgtgcccac
     2581 aacaaggtgg tccgcgacca ggtcaaagat cacgccggat tcgaggtgaa gtcccagggc
     2641 gacggattca tgctcgcttt tccctcggca aaggaggcgc tgcgttgcgc catcgggatc
     2701 cagcgggccc tggccaccag agaaccgaac ggcgtggttc tgaga
//
LOCUS       DASTXO010000213         3703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_455021, whole genome shotgun sequence.
ACCESSION   DASTXO010000213 DASTXO010000000
VERSION     DASTXO010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455021"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VFU96_04490"
     CDS             <1..339
                     /locus_tag="VFU96_04490"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU5112552.1"
                     /translation="LLMSLGSLVTMGRYGPPNLLVLLLENRSYEMTGGQPLAQKVDFV
                     ALARGAGVGKVERIDDLSVFETELPRLLTEPGPHFVVLPVTNSEPLPPVAHTDHAERV
                     VSLRRGLGLE"
     gene            complement(429..1496)
                     /locus_tag="VFU96_04495"
     CDS             complement(429..1496)
                     /locus_tag="VFU96_04495"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5112553.1"
                     /translation="MHGVSEWWPELGPKWRRSSAPKPPYPTIDIHTHVEVPAAAEIAA
                     PFFRPEYDPRMQFQPEESTRYNRELRATQTAQFIDIGTRLADMDLQGVDIQALAIAPP
                     QYFHWLDDDNGPRVNAMQNDAIFEMIREAPDRFVGIANLPMDHPQAALAEMRRVNADL
                     GFNGFEVNADVRGGDLDDRRFDPIWAAAEELEMLVILHPHGWTEPRRMDDYYLINVVC
                     MPLASTVAVSRMILGGVWERFPDLRMMVVHGGGYLPFYFARTDHAYKVRPEARRHISR
                     PPSEYLHKLYFDTTVFSTDAVQYLVGEFGADRVLMGTDYPFDMGPTDPLGFLAGARLT
                     EDQHALVVGGNAARLLRIPTR"
     gene            1540..2220
                     /locus_tag="VFU96_04500"
     CDS             1540..2220
                     /locus_tag="VFU96_04500"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU5112554.1"
                     /translation="MSDAEKTETIAAESYAAVLRPTARPRTATSYVSDSLRRSILNGD
                     LVGDTKLSLVEIASTFGVSTTPVREALRELSFEGLVTIDNYRGGVVTAVTKPEVEEIV
                     RIRQVLEPIAARESVAEMTPVILGEAQTILDEMAASDSWDVWVQGNRAFHRKIYEASP
                     SRRLVSLIKTLQDTQVVFVSATLRKSPMLKETATRDHHEMIDAIEAGDADRLIEITLR
                     HLTIPIRD"
     gene            2257..3174
                     /locus_tag="VFU96_04505"
     CDS             2257..3174
                     /locus_tag="VFU96_04505"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5112555.1"
                     /translation="MTSNPIAFIGLGLMGSRMSRHLLAAGHELRGYDPDQMRLDEFES
                     AGGVATKSPADAVLGCWAAVLSLPTSDVSRKVCLGENGIAAAGVSPLLVYDTTTGRPG
                     DALEIAAGLDQVEVDYSDTTVSGNSEVAEKGELVVMLGGSANAYVKGRPIFEAIGRSH
                     HHVGPVGSASRMKLIVNHALTVHRMALAEALVVAELAGMDLDTALEVLKDSLAYSKAM
                     DVWGDRMVVGDHEFPYARLRQSHKDARLIVEHGRELGATMDLARVAQAALAEGEETGL
                     ADMDNSSIMEVVRRRAGIGREQSDGRPVA"
     gene            3207..>3703
                     /locus_tag="VFU96_04510"
     CDS             3207..>3703
                     /locus_tag="VFU96_04510"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5112556.1"
                     /translation="MSGDGRVIDIHCHRECGSAAALVQPEAERLGRKPLHFGNEMTKE
                     VNRRQLEDVRPKMEDVEVRLADMDAMGVDVQVISLSPYQLFHWAGGELGIRAFQTIND
                     DLTDLVAGHPDRFLGLAAIPLQEPSAAIAELRRCSAELGFRGIEMATHIAGEELSSPR
                     FEDFWA"
BASE COUNT          670 a         1111 c         1241 g          681 t
ORIGIN      
        1 ctgctcatga gcctcggctc gctggtcacc atgggccggt acggcccgcc gaatctgctg
       61 gtgctgctct tggagaatcg cagctacgag atgactgggg ggcagccact ggcacagaag
      121 gtggactttg tggcgctcgc ccgaggcgcc ggggtgggca aggtggagcg aatcgacgac
      181 ctgagcgtgt tcgaaacaga gctgccgcgt ctcctcacgg aacccggtcc ccatttcgtt
      241 gtcctgcccg tcaccaactc cgagccgcta ccgccagtgg cccacaccga ccacgccgaa
      301 cgcgtggtca gcctgcgccg ggggctcggg ctggagtgac ctcatggtcg ggccgggacg
      361 aaacccgagc tcccaagaac cgggcctcaa tggctggggt ctccaggttg agcgatgacc
      421 ttcgggtgct accgggtggg gattcgcaac aatcgcgccg cgttgccacc gacgacgagg
      481 gcatgctgat cctcggtgag gcgggctccg gccaggaatc caagggggtc ggtgggaccc
      541 atgtcgaagg ggtagtcggt ccccatgagg acacggtccg ccccgaactc gccgaccaga
      601 tactggaccg catccgtcga gaacaccgtc gtatcgaaat agagcttgtg caggtactcg
      661 ctggggggtc gggaaatgtg acgtcgcgcc tccggtctca ccttgtaggc atgatcggtg
      721 cgagcgaagt agaagggcaa gtacccaccc ccgtgcacga ccatcatgcg taggtcgggg
      781 aatctctccc acacaccgcc caggatcatc cgtgacaccg ccacggtcga cgccagtggc
      841 atgcacacca cattgatcag gtagtagtcg tccatccgcc ggggctctgt ccaaccgtgg
      901 gggtggagga tcaccagcat ctccagctct tcggcggctg cccagatcgg atcgaaccgt
      961 cggtcgtcga ggtcgcctcc tcttacgtcg gcgttcacct cgaagccgtt gaagcccaga
     1021 tcggcgttga cgcgtcgcat ctcggcgagc gctgcctgag gatgatccat ggggaggttg
     1081 gcgatgccga cgaatcggtc cggtgcctct cggatcatct cgaagatcgc gtcgttctgc
     1141 atggcgttga ctcgagggcc gttgtcatca tcgagccagt ggaaatactg cggcggggcg
     1201 attgcaagcg cctggatgtc gacaccctgg aggtccatgt cggcgagtcg cgtcccaata
     1261 tcgatgaatt gggcggtctg agtcgccctg agctcgcggt tgtagcgggt gctttcctct
     1321 ggttggaatt gcatcctggg gtcgtactcg ggtcggaaga acggcgcagc gatctctgcc
     1381 gccgctggta cctcgacatg ggtgtgtatg tcgatcgtgg gatacggtgg cttgggcgcc
     1441 gacgaccttc gccacttcgg tcccagctcc ggccaccact cggaaacacc atgcatattg
     1501 cactatgtac catctccgca tgacccgctg ccaatcacga tgagcgacgc agagaagacc
     1561 gagacgatcg ccgccgaatc gtatgcggca gtcctgcgtc cgaccgcacg accacgcacc
     1621 gcgaccagct atgtatcgga ttcgctacgc cgatccatac tcaacggtga tctggtcggc
     1681 gacaccaagc tgagcctggt cgagatcgca tcgacgttcg gtgtcagcac cacaccggtg
     1741 cgtgaggcgc tgcgcgagtt gtctttcgag ggtctcgtca cgatcgacaa ctatcggggc
     1801 ggggtcgtga ccgcggtgac caagcccgag gtggaggaga tcgtgcgaat ccgccaggta
     1861 ctcgagccga tcgcggccag agagtcggtt gccgagatga cacccgtgat cctgggtgaa
     1921 gcgcagacga ttctcgacga gatggcagcc tcggactctt gggacgtctg ggtgcaaggg
     1981 aatcgggcat tccatcgcaa gatctacgag gcgtcgccgt ctcggcggct ggtgtcgctg
     2041 atcaagacct tgcaggacac acaggtcgtg ttcgtgagcg caacgctgag gaagagcccg
     2101 atgctcaagg agacggcgac gagggatcac cacgagatga tcgacgcgat cgaagcaggc
     2161 gacgccgatc gtctcatcga gatcacactg cggcacttga cgatcccgat cagggattga
     2221 gacctcgacg gtgcggggcc tgttgaaagt ggagacatga cgtcgaaccc gattgctttc
     2281 atcgggttgg gtctgatggg ctcgcggatg tctcgccact tgctcgccgc gggtcatgag
     2341 ttgaggggtt atgaccccga ccagatgcgg ctagatgagt tcgagtcggc gggaggtgtg
     2401 gcaaccaaat cgcctgcgga cgcagtgctt ggttgctggg ctgccgtgct ctcgctcccg
     2461 acgtccgatg tctcccggaa ggtgtgcctg ggtgaaaacg ggatcgccgc tgccggggtc
     2521 tcgccgctcc tggtgtacga cacgaccacc ggtcgccccg gcgacgctct cgagatcgcc
     2581 gcaggtctcg accaggtgga agtcgactac tccgacacca cggtcagcgg gaacagtgag
     2641 gtcgccgaga agggtgagct cgtcgtgatg ctgggcggat cggcgaacgc ctacgtgaag
     2701 ggacggccga tattcgaggc gattgggcgc tctcatcatc atgtcggtcc ggtgggctcg
     2761 gcctcccgga tgaagctgat cgtgaaccat gccttgactg tgcatcggat ggccttggcc
     2821 gaggccctgg tcgtcgccga gctggccggt atggatctcg acaccgccct cgaagtgctg
     2881 aaagactcct tggcatattc caaggccatg gacgtttggg gcgatcgcat ggtggtgggc
     2941 gaccacgagt ttccctatgc acggctccgg cagagccaca aggacgctcg gctcatcgtc
     3001 gaacacggcc gggaactggg tgcgaccatg gacctggccc gggtcgcgca ggccgcactg
     3061 gccgagggag aggagaccgg tctggcggac atggacaact cctcgatcat ggaggtagtc
     3121 cggcgcaggg ccgggattgg ccgggagcag tcggatggcc gaccggtggc ttgatcctat
     3181 attgtgcatg atatgaggac agacccatga gcggcgacgg tcgcgtcatt gacatccact
     3241 gtcatcggga gtgcggttcg gcggcagctc tggtccagcc cgaagcggag cgtctcggtc
     3301 gaaagcccct ccatttcggc aacgagatga ccaaggaggt caaccggaga cagctcgagg
     3361 atgtccgccc caaaatggag gatgtcgagg tccggctggc cgatatggac gcaatggggg
     3421 tggacgtcca ggtgatcagc ctctctccgt atcagctgtt ccactgggcc gggggagagc
     3481 tgggcatcag ggccttccag acgatcaatg acgatctcac cgatctcgtc gccgggcacc
     3541 cggaccgttt cctcggtctg gccgcgatcc ctttgcagga gccctccgcg gccatcgccg
     3601 agctgcggcg ctgctcggcc gagctcgggt tccgcggcat cgagatggcg acccacatcg
     3661 cgggcgagga gctgtccagc cctcgcttcg aggatttctg ggc
//
LOCUS       DASTXO010000214         2871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_234231, whole genome shotgun sequence.
ACCESSION   DASTXO010000214 DASTXO010000000
VERSION     DASTXO010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234231"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1771)
                     /gene="thrS"
                     /locus_tag="VFU96_04515"
     CDS             complement(<1..1771)
                     /gene="thrS"
                     /locus_tag="VFU96_04515"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU5112557.1"
                     /translation="MSLELRYPDGTVVEHPDDATGLQVAESIGPGLARSAVAVKINGE
                     LRDLSHPLDESGEFSVITLDSEEGLDILRHSSAHVLAQAVLDLFEGSTFAIGPAIEDG
                     FYYDFKVGEPFTPDDLERIEARMKEIISEDQPFEREALSTSDALALFASHPFKTEIIE
                     SVEPSELTPGEDVTVYRNIGFVDLCRGPHLPSTGRIPAVKLLRSSGAYWRGDEKRDQL
                     QRIYGTAWPSAKDLDAHLTQLEEAEKRDHRRLGAELDLFSFPPELGSGLVVWHPKGGM
                     LRKLVEDHSRALHERFGFDFVFTPHLAKSDLWWTSGHLEYYADYMFPGMELDNDQEYR
                     VKPMNCPFHILVYKSRARSYRDLPIRLSELGAVYRYEKSGVVHGLLRARGFTQDDSHT
                     FCTGEQLGSELALHLDFVLTWLRDAGFTEFEADLSTQPDKAPPGEQDRWDFAEKTLAR
                     ALEGSGVNFRIAPGEGAFYGPKIDMHVRDAIGRRWQLSTIQLDLILPERFGLEYTAAG
                     NVSERPYMIHCAKAGSLERFMGVLIEHHAGAFPMWLAPVQVSVVPVADRHHDYGEQVA
                     ERLRDAGLRVEVHTSDDTVGEKIR"
     gene            1874..2701
                     /locus_tag="VFU96_04520"
     CDS             1874..2701
                     /locus_tag="VFU96_04520"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEU5112558.1"
                     /translation="MDARELATKLHPNALVRELSSGANKVFRVIDDEVSTIVKVYPVS
                     SRERRERHALEALAAIPSVPRIINHGTEGEMAWLEMNDGGHWNLANLPKNLDSIEASG
                     AALRDIHTSNANITNLAAGIDADYVLSHYRSTIERLGRYRRRFGMDQAVLDKALELEP
                     PIATAPAPAHTKPTLSKFIINDEGTITIVDWEWATLAPPEWDLTLAVWRLAARIGEDA
                     SAAFRIGYGAELPESRYRPWVAYHAAMMMLEAAEVREGRLGDLGYLVDDLTEAVLGT"
BASE COUNT          560 a          893 c          909 g          509 t
ORIGIN      
        1 tgcggatctt ctcgccgacc gtgtcgtcgg acgtgtgcac ctccacccga agaccggcat
       61 ctcgcagccg ctccgcaacc tgctccccat agtcgtgatg ccggtcggct accgggacta
      121 cagagacttg aactggtgcg agccacatcg ggaatgcgcc ggcgtgatgc tcgatcagca
      181 cgcccatgaa ccgctcgagc gacccggcct tggcgcaatg gatcatgtaa gggcgttctg
      241 acacgttgcc ggcggcggtg tactccaggc cgaatcgctc gggaaggatc agatcgagct
      301 ggatcgtgga aagttgccac cgccggccga tcgcgtccct gacatgcatg tcgatcttcg
      361 gcccgtagaa ggcgccttcc ccaggagcaa tcctgaaatt gactccagac ccctccagcg
      421 cccgcgccag tgtcttctcg gcgaagtccc atcgatcctg ttctccggga ggggccttgt
      481 ccggctgggt gctcaagtcg gcttcgaact cggtgaatcc ggcatcccgg agccaggtca
      541 gcacaaagtc gagatgcagg gcgagctcgg atccgagctg ttcgccggtg caaaacgtgt
      601 gagaatcgtc ctgcgtgaag ccccgggccc ggagcagacc gtggacgaca ccggatttct
      661 cgtagcgata gaccgcccca agctcggaga ggcggatggg cagatctcga tacgacctcg
      721 ctcggctctt gtagaccagg atgtggaacg ggcagttcat cggtttcacc cggtattcct
      781 ggtcgttgtc gagctccatg ccggggaaca tgtagtcggc gtaatactcg agatgtcctg
      841 aggtccacca caggtccgac ttggcaagat gaggtgtgaa cacgaagtcg aagccgaagc
      901 gctcatgcag ggcgcggctg tggtcctcga cgagtttgcg aagcatgcct ccctttggat
      961 gccacacgac caggcctgag ccgagctcgg ggggaaagct gaacagatcg agctcagcgc
     1021 ccaaccggcg atggtcgcgc ttctcagcct cttccaactg ggtcaggtgg gcgtccaggt
     1081 cctttgccga cggccaggcg gtcccgtaga tccgctgcag ctgatcgcgt ttctcatcac
     1141 ctctccagta ggccccggag ctccgaagca acttcaccgc cgggatccgc cccgtggacg
     1201 ggaggtgagg gccccggcac agatcgacga agccgatgtt ccggtagacc gtcacgtctt
     1261 cgcccggtgt caactcactc ggctccacgc tttcgatgat ctcggtcttg aaggggtggg
     1321 aggcgaacaa cgccagagca tcggatgtgg agagtgcttc tcgctcgaag ggctggtctt
     1381 cggagatgat ctccttcatc cgggcttcga tgcgctccaa atcgtcgggg gtgaacggct
     1441 cgcccacctt gaagtcgtag tagaagccat cctcgatcgc gggcccgatg gcgaaagtag
     1501 agccttcgaa gaggtcgaga accgcctgcg ccaacacatg cgccgaagag tgacggagga
     1561 tgtccaggcc ttcctcgctg tcgagagtga tcaccgagaa ttcgcccgat tcgtcgagcg
     1621 gatggctcag atctcggagc tcgccgttga tcttgaccgc aaccgcggat cgggcgagac
     1681 cgggcccgat ggactcggcg acctggagac cggtggcatc atcggggtgc tcgaccacgg
     1741 ttccatcggg ataacgaagc tctagtgaca cggggttatc tcgctagcct ggggcgggca
     1801 cagaaggcta actggctcga agacgcaacc gggccggtga tccggtcacc cgtgacaggg
     1861 ggaacaacag cgcatggatg ccagagagct cgcgacgaag cttcacccga atgcactggt
     1921 cagagagctg tcttcgggag caaacaaggt tttccgggtc atcgacgacg aagtgagcac
     1981 gatcgtgaag gtatatccgg tgtcgtcacg tgaacgtcgg gaaaggcatg ctctcgaggc
     2041 actcgccgcc atcccgtcgg tgccccggat catcaaccat ggcaccgaag gcgagatggc
     2101 ctggctcgag atgaacgacg gagggcactg gaatctggcc aatctgccca agaacctgga
     2161 ttcgatcgag gcatccgggg cggcgttgcg cgacatccac acctccaatg ccaacatcac
     2221 caacctggcc gccgggatcg atgccgacta cgtgctgagc cactaccggt ccacgatcga
     2281 gcgtctcggt cgataccgcc gccgattcgg gatggaccag gcggtgctcg acaaggcgct
     2341 cgaactggag ccacccatcg ccacggctcc cgcgcctgcc cacaccaagc ccactctgtc
     2401 caagttcatc atcaacgacg aggggaccat cacgatcgtc gattgggaat gggccacgct
     2461 ggcaccgccc gaatgggatt tgacgctcgc cgtgtggcgt ctggctgccc gcatcggaga
     2521 agacgcctcg gccgcattcc gcatcggata cggggcggag ctccccgaga gtcgctatcg
     2581 accgtgggtc gcctatcacg cggcgatgat gatgctcgag gcagccgagg tccgcgaggg
     2641 ccgtctgggc gatctcggct atctcgtcga cgatctgaca gaagccgtcc tcgggacgtg
     2701 atacccaggg ggacgacccg ccccccacca cgtgcggcca aatccacggt tttcgcgatc
     2761 tcctctctta gcctcaccac gctgaagcaa ggaggacgga cgtgttggaa gcactggtcg
     2821 cggtcacggc gacgacaggt ctgttcgccc tggcgaaggg gataaggggc a
//
LOCUS       DASTXO010000215         2926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_381825, whole genome shotgun sequence.
ACCESSION   DASTXO010000215 DASTXO010000000
VERSION     DASTXO010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381825"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VFU96_04525"
     CDS             complement(<1..812)
                     /locus_tag="VFU96_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013807424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5112559.1"
                     /translation="MTDRIGDTVVEVRDLRMRYGSNDVLDGVEFDIRRGEVVTLLGPN
                     GAGKTTTIEILEGFRLRSDGDVSVLGVDPAQADEDWRARVGVVLQSWRDHARWTPRLL
                     LHYLGRYYEPYSTTERQRPYDVDQLIKLVGIEEQADQKIRTLSGGERRRLDVAVGIIG
                     RPELLFLDEPTAGFDPQARRDFHDLIHRLTDLEDTTVLLTTHDLDEAEKLADRILILA
                     AGRIVADGNADQLARMVTREAQVRWTRDGDQFVHATPEPTAFVRELLGQYDDV"
     gene            complement(884..1627)
                     /locus_tag="VFU96_04530"
     CDS             complement(884..1627)
                     /locus_tag="VFU96_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019044733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEU5112560.1"
                     /translation="MSDAGRVRVEPSAKRVRVVLGGEVIADTSRPLLVWEVPYFPTYY
                     FPPEDVRTEFLTPTGEVKRSPSRGDATQYSVNVGDSSGAAYSFHDSRIPECLDHYVFV
                     WRTMDHWYEEDEEVFVHARDPYTRLDILPSTRRVRVVIDGVTVADSTNGRFLFETGLP
                     ARYYLPKEDVRMDLLTATDKVTACPYKGIARYWSATVNGVTHENILWGYDDPFPESER
                     IRGLVSFYNEKTDIYIDEVRQERPKTKFS"
     gene            complement(1667..2689)
                     /locus_tag="VFU96_04535"
     CDS             complement(1667..2689)
                     /locus_tag="VFU96_04535"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEU5112561.1"
                     /translation="MTVLQATDRLADALVMPLPSFRLHPGEPLGKGLKRLGVEAIDEA
                     ISGFYEGEEMFGEAVHTTRKSTKRTRAILRLVRYEVGEKVYRFENAWMRDTARLLSEV
                     RTSSVMVQGVDDIRAMYGPLLAEGTFDEVLERTTTYRDRIEQRVMEDPGIVPRVVSNL
                     ERARGRYESWPTDPDARSVYGIGIRDDYRAIGPGLKATHARGRHQMVAAYQSPSPASF
                     HRWRKRVKYLRHQIEILTPLWPEVMIGMAVTLDRISELLGQDHDLAELLQTLADRPDL
                     CPNPMERSLMSALAEQRRADLQTASRILGRRIYAETPSALASRMSAYWESAELARKAQ
                     LVSLSA"
     gene            2714..>2926
                     /locus_tag="VFU96_04540"
     CDS             2714..>2926
                     /locus_tag="VFU96_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEU5112562.1"
                     /translation="MRSAGLLPYRLDGELRLLVAHPGGPFFARRDNGWWSIVKGIVES
                     DESDEEAAAREFEEETGWAAPPQPWIE"
BASE COUNT          526 a          898 c          952 g          550 t
ORIGIN      
        1 acgtcgtcgt actgccccag cagctcgcgg acgaacgcgg tcggctccgg tgtggcgtgg
       61 acgaactggt cgccgtcacg ggtccaacgc acctgcgctt cacgggtcac catccgggcc
      121 agttggtcgg cgttcccgtc ggccacgatc cgacccgcgg ccaggatcag aatgcgatcg
      181 gccagtttct ccgcctcgtc gaggtcgtgg gtggtgagga ggaccgttgt gtcctccagg
      241 tcggtgagac gatggatcag atcgtggaag tcccgccgag cctgagggtc gaatccggca
      301 gttggctcgt cgaggaaaag gagctcgggg cgcccgatga ttcccaccgc gacgtcgaga
      361 cggcgacgct cgcccccgga gagggtgcgg atcttctggt cggcctgctc ctcgatcccg
      421 accagcttga tgagctgatc gacgtcgtac ggacgctggc gctcggttgt cgagtagggc
      481 tcgtagtaac ggcccagata atggagcagc aatcggggag tccagcgggc atggtcacgc
      541 cacgactgga gcacgactcc gacccgcgcc ctccagtctt cgtctgcttg agccggatcc
      601 accccgagga ccgacacgtc gccgtcggaa cggagtctga agccttcgag gatctcgatg
      661 gtggtggtct tgccggctcc gttggggccg agcagtgtca ccacctcgcc acgtctgatg
      721 tcgaattcga ccccgtcgag aacatcgttg gacccgtagc gcatccgcag gtcgcgaact
      781 tcgaccacag tgtcgccgat acgatctgtc atgatccctg actttccgta ctgactagat
      841 gacggaccat agtaggaatg ctatgaacag tgcaatcgga tcctcaagag aattttgtct
      901 tcggtctctc ctgacggacc tcgtcgatgt agatgtcggt cttctcgttg tagaaggaga
      961 ccaggccgcg gatccgctcc gactcgggaa acgggtcgtc gtagccccac aggatgttct
     1021 catgtgtgac gccattgacc gtcgccgacc agtaacgcgc aatgcccttg tagggacatg
     1081 cagtgacctt gtcggtggcg gtgaggaggt ccatcctgac gtcctctttg ggcaggtaat
     1141 agcgcgcagg cagaccggtc tcgaagagga aacgaccatt ggtgctgtcg gcgacggtga
     1201 caccgtcgat cacgacgcga acccgtcgcg tcgagggcag gatgtcgagg cgggtgtaag
     1261 gatctcgagc gtggacgaag acctcctcgt cctcctcgta ccagtggtcc atggtcctcc
     1321 atacgaacac gtagtggtcg aggcattcgg gaatccggga gtcgtggaac gagtaggccg
     1381 cccccgatga gtcgccaacg ttgactgaat attgagtcgc gtcacccctg ctgggggatc
     1441 gcttcacctc accggtcggg gtgaggaact cggtccggac atcctcaggt gggaagtagt
     1501 aggtcgggaa gtatggaacc tcccagacga ggagaggtct gctggtgtcg gcgatcacct
     1561 cgccgccgag cacgacccgg acccgcttcg cactcggctc cacccggacg cggccggcgt
     1621 cgctcatgac acgagggtac aaccgcgagg cgattgcgac ggcaggtcag gcggataggg
     1681 agacgagctg cgctttacgg gcgagctccg ccgactccca atatgcgctc atgcggctcg
     1741 ccaaggccga gggtgtctcc gcatagatgc gtcggcccag tatgcgggag gccgtctgga
     1801 ggtcggcgcg gcgctgctcg gcgagcgcgc tcatcagcga acgctccatc ggattggggc
     1861 acagatcggg ccggtcagcc agggtctgga gcaactctgc gagatcatgg tcctggccga
     1921 gaagctcgga gatccggtcg agggtgaccg ccatcccgat catgacttcg ggccacagcg
     1981 gggtcaggat ctcaatctga tgcctcaggt atttgacccg cttccgccac cggtgaaaag
     2041 acgccgggct cggagattga taggccgcca ccatctgatg ccgcccccgg gcatgtgtgg
     2101 ctttcagacc gggcccgatg gcccggtagt cgtcccggat gccgatgcca tagaccgatc
     2161 ttgcgtcggg gtcggtcggc cacgactcgt aacggccgcg tgccctttcc agattcgaca
     2221 ccacccgcgg gacgattccg ggatcctcca tcacccgttg ctcaatgcgg tcgcgatagg
     2281 tcgtcgtccg ctccaggacc tcgtcgaatg tgccttctgc caaaagcggt ccatacatcg
     2341 cgcggatgtc gtcaacgccc tgaaccatga ccgaggacgt gcggacttcg gagaggagac
     2401 gggcggtgtc gcgcatccac gcattctcga accggtagac cttctcgccg acttcgtagc
     2461 ggacgagtcg gagtatggct ctggttcgct ttgtggactt ccgggtggtg tgcaccgcct
     2521 ctccgaacat ctcctcgcct tcatagaagc ctgagatcgc ctcatcgatc gcctcgacac
     2581 cgagacgctt gagacccttc ccgagtggct caccaggatg aagcctgaac gatggcaggg
     2641 gcatgaccag ggcatcagcg agccggtccg tggcctggag cacagtcacc cggccagcgt
     2701 aatgttgggc tggttgagat ccgcgggtct tctcccctat cgcctggacg gcgagttacg
     2761 tcttctggtc gcccatccag gagggccctt tttcgcccgt cgtgacaatg gctggtggtc
     2821 gatcgtgaag gggatcgtcg aatcggacga gagcgacgaa gaggccgccg cccgcgaatt
     2881 cgaggaggaa acggggtggg ccgcccctcc ccagccctgg atcgaa
//
LOCUS       DASTXO010000216         4225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_823694, whole genome shotgun sequence.
ACCESSION   DASTXO010000216 DASTXO010000000
VERSION     DASTXO010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823694"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="VFU96_04545"
     CDS             complement(<1..963)
                     /locus_tag="VFU96_04545"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010230140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEU5112563.1"
                     /translation="MAFTAVELIEAKRDHRELSPEAIAWLIGAYTDGDVPDYQMAAMA
                     MAIFLNGMSGSELAAWTSAMLHSGAVFDLSDIGAAKVDKHSTGGVGDKISIPLAPLVA
                     SVGVMVPMMSGRGLGHTGGTLDKLETIPGFTTRLEPDRFREVLSAQGMVFAGQTETLV
                     PADRKLYALRDATATVPSLPLISSSIMSKKLAEGLNGLVLDVKTGSGAFMKDLEGSRL
                     LARTMVDIGIRHGVRTIALITGMDQPLGREVGNANEMKESIAVLRGQGPDDVVELTFA
                     LGEIMMELAGIDGGRAALSEAIDSGKAFAKLIEVTKAQGGDESFI"
     gene            complement(989..1621)
                     /locus_tag="VFU96_04550"
     CDS             complement(989..1621)
                     /locus_tag="VFU96_04550"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU5112564.1"
                     /translation="MATPTRERDDVRAELVEAAIRLLADQGTEALVLRRVASAAHVST
                     MCVYSRFGDKVGLLNAVFAAGFERLSSAMSTVEHPQEPLRRIVDLWLAYRRFAVSNPA
                     LYSLMFERVIGFDPRSELRSDALESTFSMVFDAVNQAMEQGAIDAESPRAAAYTLWTA
                     AHGAVSLELTHAARRALPGWPIDIPDAGGDVFRRVLETTLKGLAPSEPAA"
     gene            1698..2690
                     /locus_tag="VFU96_04555"
     CDS             1698..2690
                     /locus_tag="VFU96_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU5112565.1"
                     /translation="MGRRTNLALAVLLVTAVVSGLASQAIGVDWPLDTAVIHGAVALA
                     ILLLSPWKTTIVRRGLRKKRPGRVFSLVLLTVVLITLASGLLHAHGEISRIGPLTVMQ
                     IHVGGAVLALGLAIDHFRRHPVTPRHFDVDRRAFLRTSALSAVAAGIWLAWEGTLQAL
                     EAPGSERRFTGMHERGSHDPAGLPTTSWLDDSVPTIDPAEWSAEVAGQVVTLADLEAM
                     PQDTFPAILDCTGGWYSEQEWTGVRLDRLIDPEGNRSFVAWSATGYARRFPIADLEST
                     WLVTRLGGESLSPGHGFPARIVAPARRGFWWVKWVTRIEPSPVPWWLQSPFPLT"
     gene            complement(2698..3534)
                     /locus_tag="VFU96_04560"
     CDS             complement(2698..3534)
                     /locus_tag="VFU96_04560"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEU5112566.1"
                     /translation="MTYANPDALVTTDWLKANLSDPNIRVIEVDEDATVYDKDHIPGA
                     IAWSWSDDLHHPTRRDYLDQEGFSKLLSTAGVGPDTTVVLYGGNNNWFAAYAYWLLKY
                     RGFDNVKLLDGGRKKWQIESLPLESDLPELTPTDFSITGAERPEVRAFRDRVLEVQGQ
                     ATLVDVRSPEEFSGEKIAPDHLPQEQPYVAGHVPGAKNIPWGKAANEDGTFKSFDELK
                     ELYEGAGVAGGEIIAYCRIGERSSHTWFVLTELLGHEDVKNYDGSWTEYGSLVNVPVE
                     RG"
     gene            complement(3742..>4225)
                     /locus_tag="VFU96_04565"
     CDS             complement(3742..>4225)
                     /locus_tag="VFU96_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEU5112567.1"
                     /translation="VFGVSGYSGAGTTPGPRNDPERLRDNLMPYKLAGHNHEKETVRH
                     LGIPVRFFPHVHPAFRGLLVTAHIPLGRPRAPEEIRARYEERFDGEPLIEIRDAIPEM
                     RDGAGQAGVILGGFETSADGLNAVVVAAEDNLLKGAAVQAIQNINLALGLGEFDGLTR
                     "
BASE COUNT          702 a         1331 c         1389 g          803 t
ORIGIN      
        1 gatgaacgat tcatccccac cctgcgcctt cgtcacctcg atcagcttcg cgaaggcctt
       61 ccccgagtcg atcgcttcgg atagggcggc ccggcctccg tcgatgccgg ccagctccat
      121 catgatctcg cccagggcga aggtcagctc gacgacatcg tccggcccct gacctcgcag
      181 cactgcgatc gattccttca tctcgttggc gttcccgact tcccgcccga gcggctggtc
      241 catcccggtg atgagggcga tcgtccgcac cccgtgtctg atgccgatgt cgaccatggt
      301 ccgagccagc aatcgcgagc cttccaggtc cttcatgaac gcgccgctgc ccgtcttgac
      361 gtcgaggacg aggccgttga gcccttccgc cagcttcttc gacatgatcg acgatgagat
      421 gaggggcagc gaggggaccg tggctgtggc gtcgcgaagg gcgtagagct ttcggtcggc
      481 agggaccagg gtttccgtct gcccggcgaa gaccatcccc tgagcggaaa gcacctcgcg
      541 gaatcgatcc ggctcgagcc tggtggtaaa cccggggatc gtctcgagtt tgtcgagtgt
      601 cccgccggta tgccccagac cacgtcccga catcatcggc accatcaccc cgaccgaggc
      661 caccaggggg gcaaggggta tcgagatctt gtctccgacg cccccggtcg agtgcttgtc
      721 gaccttggcg gcgccgatgt cgctcaggtc gaagaccgct cctgagtgga gcatcgcgct
      781 cgtccaagcg gccagctcgg atccgctcat gccattgaga aagatggcca ttgccattgc
      841 ggccatctga tagtccggga cgtccccgtc ggtgtacgcc ccgatcaacc acgcgatggc
      901 ctcgggactc agctcccgat gatctctctt ggcctcgatc agctcgactg cggtgaacgc
      961 cattcggcga gacgttatat ggatgtgact aggcggcggg ctcggatggg gcgagccctt
     1021 tgagcgttgt ttcgagtact cgacggaaga cgtccccccc ggcgtcggga atgtcgatcg
     1081 gccatcctgg gagcgctcgc cgggcagcgt gggtgagctc caggctcacc gcgccgtgtg
     1141 ccgccgtcca cagggtgtat gccgcggcgc gagggctctc ggcgtcgatc gctccctgtt
     1201 ccatagcctg gttcacggca tcgaacacca tcgagaaggt cgactccaac gcatcggaac
     1261 gcagctcgga ccggggatcg aagccgatga ctcgctcgaa catcaatgag tagagggcgg
     1321 ggttggagac cgcaaaccgg cgataggcga gccagagatc gacgatacgg cgcagtggct
     1381 cttgggggtg ctcgaccgtc gacatggccg aggacaggcg ctcgaatcct gctgcgaaga
     1441 ccgcattgag caggcccacc ttgtccccga atcgtgagta gacgcacatc gtcgacacgt
     1501 gtgccgctga tgcgacccga cgcagcacca gggcctcggt gccctggtcg gcgaggagac
     1561 ggatcgccgc ctcgaccaac tcggcccgga cgtcgtcacg ttcgcgtgtc ggggttgcca
     1621 tactcaataa cgcgcagtat aacgtgtgtt atagcgaaga tcgaatccgg ggaagacatc
     1681 gagaactgag gagggtcgtg ggtcggagaa cgaatttggc gctggcggtg ctgctggtca
     1741 cggcggtggt ctcgggtctg gccagtcagg cgatcggggt cgattggcct ctcgacaccg
     1801 cggtgatcca tggcgcggtc gctctcgcca tcctgctcct gtccccgtgg aagaccacga
     1861 tcgtgcggcg cggactgagg aagaagcgcc cggggcgcgt cttctccttg gtccttctca
     1921 ccgtcgtcct gatcacactg gcatccggtc tgcttcatgc ccatggtgag atctcacgca
     1981 tcggaccgct gactgtgatg cagatccacg tcggtggcgc cgttctggcg cttggcctgg
     2041 ccatcgacca cttccggcgt catccggtca cgccacggca tttcgatgtc gaccggcgcg
     2101 ccttcctccg aacttcggca ctgtcggccg tcgctgccgg tatctggctg gcctgggagg
     2161 ggaccctcca ggcgctggag gcgccaggaa gtgagcgtcg gttcactggc atgcatgaga
     2221 gagggtcgca tgatccggcc ggtctcccca ccacatcctg gctcgatgac agtgtgccga
     2281 cgatcgaccc tgctgagtgg tcggcggagg tagcgggaca ggtggtgacc ctggctgacc
     2341 tggaggcgat gccacaggac actttccccg ccatcctcga ctgcacggga ggctggtact
     2401 cggagcagga gtggacaggg gtcaggctcg accgattgat cgatcctgag ggcaaccgaa
     2461 gcttcgtcgc ctggtcggcg accgggtatg cccgaaggtt cccgatcgcc gacctggaga
     2521 gcacatggtt ggttacccgt ctcggcggcg agtctctgag ccccgggcac ggtttcccgg
     2581 cccggatcgt ggcaccggcg agacgtgggt tctggtgggt gaaatgggtg acccggatcg
     2641 agccctcacc ggtgccctgg tggcttcagt cgccctttcc actcacctga tcggcgatca
     2701 gcctcgctcg acggggacat tcaccaagga tccgtattcg gtccacgacc cgtcgtagtt
     2761 cttgacgtcc tcgtgcccga gcagctcggt gagcacgaac caggtatggg acgaacgctc
     2821 gccgatgcgg caatacgcga tgatctcgcc tccggcgacg ccggccccct cgtagagctc
     2881 cttcaactcg tcgaacgact tgaaggtgcc gtcctcgttg gccgccttgc cccatgggat
     2941 gttcttcgcg cccggcacat gaccagccac gtacggctgt tcctggggga gatggtccgg
     3001 agcgatcttc tcgccgctga actcctccgg tgagcggacg tcgacgagcg ttgcctgtcc
     3061 ctggacctcg aggactcggt cccgaaaggc ccggacctcg ggtcgctccg ccccggtgat
     3121 cgagaagtcg gtgggggtca gctcggggag gtccgactcg agggggagcg actcgatctg
     3181 ccacttcttg cggcccccgt ccagcagctt gacgttgtcg aagccgcggt acttgagcag
     3241 ccagtaggcg taggcggcaa accaattgtt gttcccgcca tagaggacaa cggtggtgtc
     3301 gggcccgacc ccggccgtgg acaggagttt gctgaacccc tcctggtcga ggtagtcacg
     3361 ccgcgtcgga tggtggaggt cgtcggacca gctccaggcg atagcgccgg gaatgtggtc
     3421 cttgtcgtag acggtggcgt cctcatccac ctcgatgacc cgtatgttcg ggtcgctcag
     3481 attggccttc agccaatcgg tggtgacgag ggcgtcggga tttgcatatg tcatgtgctc
     3541 tttggctcct gatgagattc gatgattggg gacgacgccg tgagggtttc gctctcgggc
     3601 ggtggcgtcg ttaggagcgc tgacgatggg tcaggtctgc gctcgggcat gccacccgag
     3661 agctgtcaca gagacacatg gacatcgtga gggacacatc gggacgttat gcacagggaa
     3721 ggtcgaagtc aatgtgacgg ctcaccgagt caggccatcg aactcgccaa gccccagagc
     3781 gaggttgatg ttctggatcg cctgcacggc ggcccctttg agcaggttgt cctcggcggc
     3841 gaccacgacc gcattcagac cgtcggcgct cgtctcgaac ccaccgagga tgacgcccgc
     3901 ttgaccagcc ccgtccctca tctccgggat cgcatcccgt atctcgatca aaggctcccc
     3961 gtcaaagcgc tcctcatatc gcgccctgat ctcctcgggt gccctgggtc gacccaacgg
     4021 gatgtgcgcg gtgacgagga gacccctgaa cgccgggtgg acatggggga agaaccggac
     4081 gggaatgccg agatggcgga cggtttcctt ctcgtggttg tggcctgcga gtttgtaggg
     4141 catgaggttg tctcggagac gctcggggtc gttgcgcggg ccgggagtcg ttccggcccc
     4201 cgagtaccca gagacaccga agacg
//
LOCUS       DASTXO010000217         4095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_308374, whole genome shotgun sequence.
ACCESSION   DASTXO010000217 DASTXO010000000
VERSION     DASTXO010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308374"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..690
                     /locus_tag="VFU96_04570"
     CDS             109..690
                     /locus_tag="VFU96_04570"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEU5112568.1"
                     /translation="MSRVLLVDNYDSFTYNLVQALRILGAEVVVRTNDRVSLDEARDL
                     EPTHLVVSPGPGRPEAAGESMKLIAGLLDVVPILGVCLGHQAIATALGGTVDHAQELR
                     HGKSSPVYHDSASIYAGLPNPFDAGRYHSLAVMEEDLPADLVVTSFTSDGVIMGIRHR
                     SLPVEGVQFHPESMLTPHGELLLRNFLDLGGIR"
     gene            687..1706
                     /gene="trpD"
                     /locus_tag="VFU96_04575"
     CDS             687..1706
                     /gene="trpD"
                     /locus_tag="VFU96_04575"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU5112569.1"
                     /translation="MSQVLEDLLSGVDLSEEEAENLLRLLTSGEVDPAVAGAILAALR
                     AKGETPEEVRGLARAMRDLATDPQVDATGAVDVVGTGGDRSGSLNLSTGSAILAAASG
                     VPVVKHGNRSMTSQSGSADVLEALGLPMPMDEAQVRRLFEATGFTFLFAPHFHPAMAA
                     IGPVRRALAVRTVFNVLGPLTNPAAPPNMVIGAFDPAAARLMAGALSGLDIERAFVVH
                     GAGGWDEPSPLGEFILFDVTPGAVKESVRHPADIGMAGCEMDDLTGGSPEHNARRLLD
                     VLEGEQGPHRDSVVLGAALALEVSGRATSPDQAVAMAAAAIDDGRALSLAKALEEFGT
                     SEAAR"
     gene            1703..2512
                     /locus_tag="VFU96_04580"
     CDS             1703..2512
                     /locus_tag="VFU96_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="HEU5112570.1"
                     /translation="MSRFLATMASRSQERANEAAGRRSLRRRREAVLAGPAPRPLSGF
                     GASFDLIAEVKPRSPSEGLFPERSPVETAMAYQIGGAGMISVLTEPSEFGGSVDMLRD
                     VAAAVSIPVLAKDFLVDPIQVYEAREAGADGVLVIVRILDEESLNEILDAVSDSRLFA
                     LLEAFDGADLERISKVVAGLSGHLAGVNCRDLDTLEVVPERHVALAGDIPGDVVTVAE
                     SAIGQPADVERVASRGYRAALVGSALMKADDPALLVREMVDAGRRSVTVPS"
     gene            2509..3129
                     /locus_tag="VFU96_04585"
     CDS             2509..3129
                     /locus_tag="VFU96_04585"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEU5112571.1"
                     /translation="MKLFVKLCGITTEADLEAAVEAGADAIGLVLTPSPRQIPLSVAA
                     GLKARLPESVLGVAVFHDPTPELLRRAQEEIGPDLFQSELSTLVGISSDKLLPVVVDG
                     ESLDRDFDLAARSADREMVVVDRAARGGTGLAPRWERLAGLSGPGRLILAGGLDPENV
                     GDAVSLVKPFGVDVSSGIEKAPGKKDPKRMHAFVEAARSVWVGGSA"
     gene            3126..>4095
                     /gene="trpB"
                     /locus_tag="VFU96_04590"
     CDS             3126..>4095
                     /gene="trpB"
                     /locus_tag="VFU96_04590"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012824031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU5112572.1"
                     /translation="MTTARNETGRFGRFGGRYVPEILVPALTRLEETSRLLLDDPGFL
                     NMYEGELSSWVGRPTALTHAVRLGEVWGGELWLKREDLAHTGAHKINNAIGQALLARE
                     MGAERVIAETGAGQHGVATAAACARLGLDCTVYMGSVDIERQAPNVERMRILGAKVQP
                     VTAGDATLRAAINEALRDWVTRPETTYYLLGSAVGPHPYPWLVRELQSVIGREARRQI
                     LEQTGRLPDAAVACVGGGSNAIGLFHPLLDDPIELIGVEAGGTSPELGHNAATIGRGT
                     PGVLHGSFSFLLQDEDGQVQETHSVSAGLDYPGVGPEHSWLAETGRV"
BASE COUNT          624 a         1341 c         1434 g          696 t
ORIGIN      
        1 ggcgggtgcg ggcatagtcg ccgacagtgt cccctcggcc gaatgggagg aagtcctctc
       61 caagtccggg gcgatgcgtg gcgccctcga gaccgcggcg ggtgggctat gagccgggtg
      121 ctcctcgtcg acaattacga ctctttcacc tacaacctgg tccaggctct gcggatactc
      181 ggcgccgagg tggtggtccg caccaacgac cgcgtgagcc tcgacgaggc ccgggacctc
      241 gagcccaccc atctcgtggt ctctccgggc ccggggcggc ccgaggcggc gggcgagtcg
      301 atgaagctga tcgcgggtct cctcgacgtc gtcccgatcc tgggggtgtg tctcggacat
      361 caggcgatcg ccactgccct cgggggcacc gtggaccacg cccaggagtt gagacatggc
      421 aagtcgtcac ctgtgtatca cgactccgcc tcgatctacg ccgggctccc caacccgttc
      481 gacgccgggc gctaccactc cctggcggtg atggaggagg acctccccgc agacctcgtg
      541 gtcacctcgt tcacctccga cggcgtgatc atgggcattc ggcaccggtc attaccggtc
      601 gaaggcgtcc agttccaccc cgaaagcatg ctcacgcctc acggtgagct gctgctgcga
      661 aacttcctcg atttgggtgg gatccgatga gtcaggtgct cgaagacctg ctcagtggcg
      721 tcgacctctc agaagaggag gccgagaacc tgctgcgtct gctgacgagt ggcgaggtcg
      781 acccggcggt ggccggtgcc atcctcgccg ccctgcgggc caagggggag acgccagaag
      841 aagttcgcgg cctggcccgc gcaatgcgag acctcgccac cgatcctcag gtcgacgcca
      901 ccggcgccgt cgacgtagtc gggaccggcg gcgatcggtc ggggagcctg aatctctcca
      961 ccggatcggc catcctggcc gcggcgagcg gcgtaccggt cgtcaagcat gggaatcggt
     1021 cgatgaccag ccagagcggg agcgccgacg tgctcgaggc gttgggcctg ccgatgccca
     1081 tggacgaggc acaggtgcga cgactcttcg aggcgaccgg cttcaccttc ctcttcgccc
     1141 ctcacttcca tccggcgatg gcggcgattg gtccggtgcg acgtgccctg gcggtgcgca
     1201 cggtgttcaa tgttctcggg ccgctcacca acccggcggc accgcccaac atggtgatcg
     1261 gcgctttcga ccctgccgcg gccaggctca tggcgggagc tctctccggt ctcgacatcg
     1321 aaagggcgtt cgtggtgcac ggggcaggag gatgggacga gccgtcgcct ctgggcgaat
     1381 tcatcctctt cgatgtgaca cccggcgcgg tcaaagagtc ggtccggcac cccgccgaca
     1441 tcgggatggc cggttgcgag atggatgacc tgaccggtgg ctcgccagag cacaacgcca
     1501 ggcggttgct cgatgtcctg gaaggcgagc aaggcccaca ccgggattcg gtcgtgctcg
     1561 gggcagcgtt ggcgctcgag gtcagtggcc gcgccacttc gcccgatcag gcggtggcga
     1621 tggcggccgc cgccatcgac gacgggaggg cgctcagtct ggccaaggca ctcgaggagt
     1681 tcgggacatc ggaggccgcc cgttgagtcg cttcctcgcc acgatggcgt cccggtctca
     1741 ggaaagggca aatgaggccg ccggccgccg ctcgctgcgc cggcgtcgcg aggcggtgct
     1801 cgccgggccc gcgccgcgtc cgttgtcggg ctttggagcc tcgttcgatc tcatcgccga
     1861 ggtgaagccg cgctctccct cggagggcct cttcccggag cggtccccgg tggagaccgc
     1921 catggcctat cagatcggtg gggccgggat gatctcggtg ctgaccgagc cctccgagtt
     1981 cggagggtcc gtcgacatgc tgcgggacgt tgccgccgcg gtgtcgatcc cggtgctggc
     2041 caaagacttc cttgtcgacc cgatccaggt gtacgaggcc cgtgaggcgg gcgccgatgg
     2101 cgtgctcgtg atcgtgcgaa tccttgatga ggagtctctc aacgaaatcc tcgacgccgt
     2161 ctccgatagc cggctcttcg ccctcctcga ggcattcgat ggtgccgatc tggagcggat
     2221 aagcaaggtg gttgctgggc tatccggcca tctcgccggc gtgaactgcc gggacctcga
     2281 cacactggag gtcgttccgg aacgtcatgt cgcgcttgcc ggcgatatcc ctggcgacgt
     2341 cgtgaccgtt gccgagagcg cgatcgggca accggcagac gtcgagcgcg tcgcgtcccg
     2401 cggctaccgc gccgctctcg ttgggtctgc cctgatgaaa gccgacgatc cggccctcct
     2461 ggtcagagag atggtggatg ccggtcgacg cagcgtgacc gtgccgtcat gaagctcttc
     2521 gtgaaactat gcgggatcac caccgaagct gacctcgagg cagcggtcga ggcgggggcg
     2581 gacgcgatcg ggctcgtcct gaccccgtcc ccccggcaga tccccctgtc ggtggcggcg
     2641 gggctgaagg cgcgactgcc cgagtcggtt ctcggcgttg ctgtcttcca cgaccccact
     2701 ccggagctgc tgcgccgagc acaggaggag atcggccccg atctgttcca gtccgagctc
     2761 tcgacactgg tcgggatctc ctcggacaag ctccttccag tcgtggtcga cggagaatct
     2821 ctggatcggg acttcgatct cgctgcgcgc tcggccgacc gcgaaatggt cgtcgtggac
     2881 cgcgccgccc gaggcgggac gggtctggcg ccgaggtggg aacggcttgc cggactgagc
     2941 ggccctggtc gcctgatcct tgccggtggg ttggacccgg aaaacgtggg agatgctgtc
     3001 tccctggtca aaccgttcgg cgtcgatgtc agctcgggca tcgagaaggc gccgggcaag
     3061 aaggatccca agcgaatgca cgcgttcgtg gaagccgcgc gcagcgtgtg ggtgggaggt
     3121 tcggcgtgac gaccgctcgc aacgagacgg gccgcttcgg ccgcttcgga ggccgatatg
     3181 tccccgagat cctggtcccc gccctcacca ggttggaaga gacctcccgg ctgctcctcg
     3241 atgatcccgg cttcctcaac atgtacgaag gggagctgtc ctcttgggtg ggtcggccga
     3301 cggccctgac tcatgccgtc cgtctcggcg aggtgtgggg gggcgagctg tggttgaagc
     3361 gcgaggacct ggcgcacacc ggagcccaca agatcaacaa cgccataggt caggccctcc
     3421 tcgccaggga gatgggtgcc gagcgggtga tcgccgagac cggggcgggt cagcacggcg
     3481 tggcaaccgc ggccgcctgc gctcggctcg gcctggattg caccgtgtac atgggctcgg
     3541 tggacatcga acgtcaggcg ccgaatgtcg agcgcatgcg gatcctgggg gcgaaagtgc
     3601 agccggtgac agccggggac gccaccctgc gtgccgccat caacgaggcg ctgcgcgact
     3661 gggtgacacg gcccgagacc acctactacc tcctcggctc ggccgtcggt ccccacccct
     3721 acccgtggct ggtcagagag ctccaatcgg tgatcggtcg cgaggcgcgt cgtcagatcc
     3781 tggagcaaac cggtcgtctc cccgatgccg cggtcgcatg tgtcggtggg ggctccaatg
     3841 cgataggcct gtttcatcct ctgctcgacg atccgatcga gctgatcggg gtggaggccg
     3901 gtgggacgtc gccggagctc gggcacaacg ccgccacgat cggtagaggg accccggggg
     3961 tgctccacgg gagcttttcc ttcctcttgc aggacgagga cggccaggtc caggagacgc
     4021 actccgtctc ggcagggctc gactatccgg gcgtgggccc cgagcactcg tggctggcag
     4081 aaacggggag agtcg
//
LOCUS       DASTXO010000218         4309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_492615, whole genome shotgun sequence.
ACCESSION   DASTXO010000218 DASTXO010000000
VERSION     DASTXO010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492615"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VFU96_04595"
     CDS             <1..274
                     /locus_tag="VFU96_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112573.1"
                     /translation="GPLPTEAEALISLARLRVEALRVGLEELVQLRHEIRMGPVDLKS
                     SQEVRLRRLQPRSVLNAQEGVIFIPAPPDLVPGLVTFLREMWAEEA"
     gene            342..1217
                     /locus_tag="VFU96_04600"
     CDS             342..1217
                     /locus_tag="VFU96_04600"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU5112574.1"
                     /translation="MKKHAILLIIAALALAACGSGGSGGGVAATVNGDDITVEEVEAL
                     ITGDGEEIPKADFAGALGFLVQQHIVSDAAETELGIVIPEDEITAEATALFEESANGQ
                     TREEFLEAQGITEELLQQFAHSQLLVTEISESLQEDVEQPTQEQIDELYCSSHILVAT
                     EQEANDVLTRIEAGEDFAALATELSTDTGSGAQGGDLGCSAPDAYDPAFAQALTTAEV
                     DVPTDPVESEFGFHVILVRDDEEEAIETLTATAANEAANTWFTEQFEAAEVTVDETYG
                     TWQASPQPQVVPPAE"
     gene            1214..2617
                     /gene="mazG"
                     /locus_tag="VFU96_04605"
     CDS             1214..2617
                     /gene="mazG"
                     /locus_tag="VFU96_04605"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEU5112575.1"
                     /translation="MITVTGLGPGDLDRIPTAVRSILLDPGRTVILRTEEHPAASQLA
                     ALRQVVTCDDLYEKGSSFDDVYRAIADRVLEMASNGPVVYAVPGSPRVGEFAVRMLLA
                     SDAEIELIPGESFIDAVLDEVGYDPLDRGLQILNGHDLPDPLVLDKPTIVAQLDRPEV
                     LADVVAHIARVLPEAAKIVVLAGLGASDAVRIDVSPDGIDPTLAGYRTSIFIDSAPGG
                     LIGAVRTMTTLREECPWDREQTHQTLVKYLVEETYELIDAVGKLDVEETDWVAYAAVE
                     DELGDVLLSVLFHAAIARENGAFDIDDVAEMMRQKLVRRHPHVFGEVDVDSAADVKRN
                     WDQIKDEERGSGRESQMDGIPSALPAIQRASKIQNRAAKVGFDWSQAAEVLPKVREEL
                     DELEAVLTEPAEAESELGDVLFSVINLARHLGIDGELALRGAVDRFDARFRAMEGMGP
                     MAGLSLEEMNLRWEQSK"
     gene            2687..3970
                     /gene="eno"
                     /locus_tag="VFU96_04610"
     CDS             2687..3970
                     /gene="eno"
                     /locus_tag="VFU96_04610"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU5112576.1"
                     /translation="MIHHPKIERVVARQVLDSRGNPTVEAEVHTTGGGFGRGAVPSGA
                     STGTLEAVELRDGGERWAGKGVTKAVGNVVDRIAPALVGIDVTRQEHIDQTMIDLDGT
                     TTKGKLGANAILAVSLAVARAASSAVGLPLYRYLGGPAAQVLPVPCLNVLNGGAHAAN
                     SVDIQEFMVVPAGLPSYSEALRAGVEVYHALKRVIGGRGLGTNVGDEGGFAPDLPTNE
                     AALELLVEAIDVAGYRLGDEISLALDVAATEFHREGQYNLEGGEMDSAEMVEYLAGLV
                     DRYPLVSIEDGMAEDDWEGWKALTDRLGDGIQLVGDDLFVTNEEILRKGIAQGVANAI
                     LIKVNQIGSVSETLHTMAAAKGASYATMVSHRSGETEDSFISHLAVATNSGQIKTGAP
                     ARGERTAKYNQLLRIEESLGGEARFAGWDSFRRAG"
     gene            3967..>4309
                     /locus_tag="VFU96_04615"
     CDS             3967..>4309
                     /locus_tag="VFU96_04615"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU5112577.1"
                     /translation="MTLIRRPGVALVTLLFLLLGAAFITQVVPYRQIIDSQRQVTAAQ
                     ERLATLEEENAALQADAAALNTDEEIEKLAREKLGYVRPGETAYVVLDPPGATQSAAD
                     AEEEGLTVDERT"
BASE COUNT          785 a         1396 c         1410 g          718 t
ORIGIN      
        1 cggccctctg ccgacagagg cggaggcgct gatctccctg gcccggctcc gggtggaggc
       61 attgcgggtc ggcctggagg agttggtcca gctgcgtcat gagatcagga tgggacccgt
      121 cgacctgaag agctcgcagg aggtgcggct gaggcgactc cagccccgct ccgtcttgaa
      181 cgctcaggag ggtgtgatct tcatccccgc gccccccgat ctcgtccctg gtctcgtcac
      241 tttcctccgt gaaatgtggg ctgaggaggc ataggcgtgg gtccgcctac cacgtggcac
      301 cgagtaccct tccgatctcc cctctctcga aggattcggt cttgaagaag catgcaattc
      361 tcctgatcat tgctgcactc gccctcgccg cgtgtggcag cggcggcagc ggtggtggtg
      421 tggccgcgac agtcaacggc gacgacatca ccgtggaaga agtcgaagcc ctcatcaccg
      481 gcgatggtga agagatcccg aaggcagact ttgccggggc cctcggcttc ctcgtccagc
      541 aacacatcgt gtccgacgcc gccgagaccg agctcggcat cgtgataccc gaggacgaga
      601 tcaccgccga ggccaccgct ctcttcgagg aatctgccaa cggccagact cgtgaggagt
      661 tcctcgaggc gcagggcatc accgaggaac tgctccagca gttcgcccac agccagctcc
      721 tcgtcactga gatcagcgag agcctgcagg aggatgtcga acagcccacc caggagcaga
      781 tcgatgagct ctactgctcc tcgcacatcc tggtggccac cgagcaagag gcgaatgacg
      841 ttctcacccg catcgaggcc ggcgaggatt tcgccgccct cgccaccgag ctgtcgaccg
      901 acaccggcag cggagcccag ggtggcgacc ttggctgctc tgccccggat gcatatgacc
      961 ctgccttcgc ccaggctctc accacagcag aggtggacgt tcccaccgat cctgtcgaga
     1021 gcgagttcgg cttccacgtg attctggtcc gtgacgatga ggaagaggcc atcgagaccc
     1081 tgaccgccac cgcagcgaac gaggcggcca acacctggtt caccgagcag ttcgaggccg
     1141 ccgaggtgac cgtcgacgag acatacggca cctggcaggc gagcccacaa ccacaggtgg
     1201 tcccaccggc cgagtgataa cggtcaccgg cctgggtccc ggcgatctcg accggattcc
     1261 gacggctgtc cgctcgatct tgctcgatcc cggccggacg gtgatcctcc gcaccgagga
     1321 acatcccgct gccagtcagc tcgctgcttt gcggcaagtg gtcacctgcg atgacctcta
     1381 cgagaaaggc agttcgttcg acgacgtcta ccgtgcgatc gccgatcgtg tcctggagat
     1441 ggcgtcgaac gggccggtgg tctacgcggt cccgggcagc cctcgggtgg gcgagttcgc
     1501 agtccggatg ctgctggcgt cggacgccga gatagagctg atcccgggcg agtccttcat
     1561 cgatgcagtc ctcgacgagg tgggctacga cccgcttgac cgcgggttgc agatcctcaa
     1621 cggtcacgac ttgcccgacc ccctcgtgct ggacaagcca accatcgtgg cccaactcga
     1681 ccgcccggag gtcctggccg acgtggtggc tcacattgca cgggtgctcc ccgaggctgc
     1741 gaagatcgtg gtcctcgcgg gcctgggtgc ctccgacgcg gtccggatcg acgtgtcgcc
     1801 cgacggtatc gacccgaccc tcgccggtta ccggacatcg atcttcatcg acagcgcccc
     1861 ggggggtctc atcggcgcgg tccggaccat gacaactctg cgcgaggagt gtccatggga
     1921 tcgggagcag acgcaccaga cgctggtcaa gtacctggtc gaggagacct acgagctgat
     1981 cgacgcggtg ggcaagctgg atgtcgaaga gaccgattgg gttgcctatg cggcagtcga
     2041 ggacgagctg ggagatgtcc tcctctcggt cctcttccat gccgccatcg ccagggagaa
     2101 cggcgcattc gacatcgacg acgtggcgga gatgatgaga cagaagctcg tgcgtcggca
     2161 tccacatgtc ttcggggagg tcgatgtcga ctccgccgcc gatgtcaagc ggaactggga
     2221 tcagatcaaa gacgaggaga ggggctcggg gcgggagtca caaatggacg gcattccaag
     2281 cgccttgccc gccatccaac gcgcctccaa gatccagaat cgcgcggcaa aggtgggatt
     2341 cgattggtcc caggcggccg aggtcctgcc caaggtccgg gaggagctcg acgagctcga
     2401 ggcagtcctc accgaacccg ccgaggccga gtcggagctg ggcgatgtcc tgttctcggt
     2461 gatcaatctc gcccgccacc tgggcatcga cggtgagctg gccctgcgtg gggcggtgga
     2521 tcgattcgac gcccgatttc gggccatgga gggcatgggc ccgatggcag gcctgtccct
     2581 cgaggagatg aatctccgct gggagcaatc gaagtgacgg cgttccacgt tttgcgctgt
     2641 acgttgtacg gcgtcgtaga acctaagacg taggacacag gcctccatga tccatcatcc
     2701 caagatcgag cgcgtcgtcg ctcgccaggt cctcgactca cgaggaaacc ccacagtgga
     2761 agccgaggtg cacaccacgg gaggaggatt cgggcgagga gcggtgccct ccggggcgtc
     2821 gaccggcacg ctggaagcag tcgaattgcg tgatggtggc gaacggtggg cgggcaaggg
     2881 tgtgaccaag gccgtcggaa atgtcgtgga ccggatcgca cccgccctgg tcgggatcga
     2941 cgtcacccgc caagagcaca tcgaccaaac gatgatcgat ctcgacggca cgacgaccaa
     3001 gggaaagctg ggcgccaacg ccatcctggc cgtgtccttg gccgtggcca gggccgcttc
     3061 gtcggccgtc ggcctcccgc tctaccgcta tctgggtggc ccggccgccc aggtcctccc
     3121 ggtcccctgc ctcaacgtgc tgaacggcgg tgcccacgcg gccaactctg tcgacatcca
     3181 ggagttcatg gtcgtgcccg ccggcctccc gtcctacagc gaggctcttc gggccggggt
     3241 cgaggtgtac cacgccctga agcgcgtgat cgggggccgt ggcctcggga ccaatgtcgg
     3301 cgacgaggga gggttcgctc ccgacctgcc cacaaatgag gcggcgctgg aactgctggt
     3361 cgaagccatc gacgtcgccg gctaccggct cggtgacgag atctccctcg ccctagacgt
     3421 cgccgccacc gagttccatc gtgagggtca gtacaacctc gagggtggcg agatggattc
     3481 ggcggagatg gtcgaatacc tcgccggcct cgtcgatcgc tatccgctgg tttccatcga
     3541 ggatgggatg gccgaagacg actgggaggg atggaaggcc ttgaccgacc gtttgggcga
     3601 tgggatccag ttggtgggtg acgacttgtt cgtcaccaac gaggagatcc ttcgaaaagg
     3661 catcgcacaa ggcgtggcca atgccatcct gatcaaggtg aaccagatcg gatcagtctc
     3721 cgagaccctg cacacgatgg ccgccgccaa gggcgcctcc tatgcgacga tggtcagtca
     3781 tcgctccggc gagaccgagg acagcttcat ctcccacctc gccgtcgcga cgaactccgg
     3841 ccagatcaag accggtgccc ccgcccgggg cgagcgaacg gccaagtaca accaactgct
     3901 ccgcatcgag gagtcgttgg ggggcgaggc ccgtttcgcg ggatgggaca gcttccggag
     3961 ggcgggttga cgctgatacg acgccccggc gtcgccctcg tcacactcct cttcctgctt
     4021 cttggcgctg cgttcatcac gcaggtcgtg ccctaccggc agatcatcga ctcacagcgc
     4081 caggtcaccg cagcccagga gcgcctggcc actctcgagg aggagaacgc ggcacttcag
     4141 gccgatgccg ctgccctgaa cacggacgag gagatcgaga aactggctcg ggagaagctc
     4201 ggctacgttc gccccggtga gacggcatac gtcgtgctcg acccccccgg ggccacccaa
     4261 tcagccgccg acgcggaaga agaggggttg acggtcgacg agaggacct
//
LOCUS       DASTXO010000219         3460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1818586, whole genome shotgun sequence.
ACCESSION   DASTXO010000219 DASTXO010000000
VERSION     DASTXO010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1818586"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3084
                     /locus_tag="VFU96_04620"
     CDS             <1..3084
                     /locus_tag="VFU96_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEU5112578.1"
                     /translation="LLVERDAGHIRIFGGGGGTILPDEGEELVGYGVTRVYSPDDGRE
                     MGMQGMVSDMLARSDFATGELGADFELDDLDAGNTRMVATLISAAENFPEKYQDVFEQ
                     IESRAAASSAPVVGITGTGGSGKSSLVDELLRRFLLDFEDLKIAVVSVDPSKRRSGGA
                     LLGDRIRLNSIPHPRAYMRSLATRQANLALSPHVQRAIEVVKAAGFDLVLLETSGIGQ
                     SDTEIVDYSDMSIYVMTPEYGAATQLEKIDMLDFADLVAINKADKEGAHDALRDVRKQ
                     YKRNRLAFTADDNELPVHLTVASDFNDPGTNRFYRAVLGELAAHGLSLETSLDLPLGD
                     EAKQHVVPPERTRYLAEVVESNRRYDTWAGEQSEIADTLYKLEGAREHGADVADIVTE
                     AEEKLDPENRRALDHWEAMLAEYSGTEFIYHVRGREIRVPLHEETLSHTRVPKVALPR
                     YRSWGDRLRWLLQENVPGEFPYTAGVFPFKRAEEDPARMFAGEGPPEQTNRRFHYLAA
                     GMPAKRLSTAFDSVTLYGEDPHERPDIYGKVGNSGVSIATLDDAKKLYSGFDLSDPAT
                     SVSMTINGPAPMILAFFMNAAIDQACERHITENDLWAEVEARIDAIYGDRLRPVYAGD
                     LPEGHDGLGLQLLGVTGDQVLPADTYERIRAETVSRVRGTVQADILKEDQAQNTCIFS
                     TEFALGMMGDIQQWFVDNDVRNFYSVSISGYHMAEAGANPITQLAFTLANGFTYVENY
                     LARGMDIDDFAPNLSFFFSNGIDAEYAVIGRVARRIWAKAIKHRYGGNERSQKLKYHV
                     QTSGRSLHAQEISFNDIRTTLQALYAIYDNTNSLHTNAYDEAITTPTEESVRRALAIQ
                     LIINQELGLAKNQNPLQGSFIIEELTDLVEEAVLAEFDRINSRGGVLGAMERQYQRTK
                     IQEESLYYEQQKEAGAYPVVGVNTFISKEGSPFVIPRTLIRSTDEDKDRQIANVCAYQ
                     ERNAETGPQALEELQKVAVTGGNIFAQLMETAKVASLGQMSNALYGVGGQYRRNM"
     gene            complement(3085..3447)
                     /locus_tag="VFU96_04625"
     CDS             complement(3085..3447)
                     /locus_tag="VFU96_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018512947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5112579.1"
                     /translation="MSGELAFVEFGVEDVDKARAFYRGLFGWKFEPGPTGQGYVISLP
                     NVGAGIHGSDPGAAPYLFFRVEDIEAAVEKIQELGGSVDDVDVEGDEESIARFGRFKL
                     CRDDQGSPFGIHQPPLPM"
BASE COUNT          693 a         1131 c         1050 g          586 t
ORIGIN      
        1 ttgctggtgg agcgagacgc ggggcacatc cgcatcttcg gtggtggcgg cgggacgatc
       61 ctccccgacg agggtgagga gctggtcggg tatggggtca cacgggtcta ctcgcccgac
      121 gatgggcgtg agatggggat gcaagggatg gtcagcgaca tgctggcccg atccgacttc
      181 gccaccggcg aactgggggc ggatttcgaa ctcgacgacc tcgacgccgg caacacccgg
      241 atggtggcca cactcatttc ggccgcggag aacttccctg agaaatacca ggacgtcttc
      301 gagcagatcg aatctcgcgc cgcggcctcc tccgcccctg tggtggggat caccggcacc
      361 ggtggctccg ggaagtcgag tctcgtcgac gagctgttgc ggcgattcct cctcgacttc
      421 gaggatctga aaatcgccgt cgtctcggtc gatccctcaa agcggcgatc gggtggggca
      481 ttgctcggcg accggatccg gctgaactcg attccccatc cacgcgccta catgcggtcc
      541 ctggctaccc gtcaggccaa cctcgccctc tcgccacacg ttcagcgcgc aatagaggtg
      601 gtcaaagccg cggggttcga tctggtgctg ttggagacat ccggaatcgg gcaatcagat
      661 accgagatcg tcgactactc ggacatgtcg atatatgtga tgacccccga atacggggct
      721 gccacccagc tcgagaagat cgacatgctc gatttcgccg acctggtggc gatcaacaag
      781 gcggacaagg agggggctca tgacgcgctc cgcgacgttc gcaagcagta caagcggaac
      841 cgtcttgcgt tcactgccga tgacaacgag cttccggttc atctgacggt cgcctcggat
      901 ttcaacgacc cggggacgaa tcgcttctat cgggccgtcc tcggagagct ggccgcccat
      961 ggcctttccc tcgagacgag cctcgacctc cccctgggcg atgaggcgaa acagcacgtc
     1021 gtcccgcccg agcgaacccg ctacctggcc gaggtcgtcg agtcgaaccg gcgttacgac
     1081 acctgggccg gggagcagtc ggagatcgca gacacgttgt acaagctcga aggagcgcgg
     1141 gaacacggcg ccgacgtggc cgacatcgtc accgaggctg aggagaagct cgatcccgag
     1201 aaccgccggg ccctcgatca ctgggaggcg atgctcgccg agtactcggg gaccgagttc
     1261 atctatcacg tgagaggacg cgagatcagg gtcccgctcc acgaggagac tctgagccac
     1321 acccgagtcc cgaaggttgc tttgccccgc taccggtcgt ggggagatcg gcttcgctgg
     1381 ctgctgcagg agaacgtccc gggagagttt ccctataccg ccggggtgtt cccgttcaaa
     1441 cgggcagagg aagacccggc gcgaatgttc gccggggagg gaccgccgga gcagaccaac
     1501 cgtcgtttcc actacctggc cgccggcatg ccggcaaagc gcctgtcgac cgcgttcgac
     1561 tcggtgaccc tctatggcga ggacccccac gagcggcccg acatctacgg caaggtgggc
     1621 aactcggggg tttccatcgc gaccctggac gatgccaaga agctgtattc cggtttcgat
     1681 ctctccgatc ccgccacatc ggtttcgatg accatcaacg ggccggcccc gatgatcctc
     1741 gccttcttca tgaacgcggc catcgaccag gcctgtgaga ggcacatcac cgaaaacgac
     1801 ctgtgggcgg aagtcgaggc ccgcatcgat gccatctacg gggatcgtct caggccggtc
     1861 tacgccggcg atctacccga aggccacgat gggttgggtc tccagctgct cggggttacc
     1921 ggagaccagg tgctcccggc cgacacctat gagcggatca gggccgagac cgtctcccga
     1981 gtccgcggca cggtgcaggc ggacatcctc aaagaggacc aggcccagaa cacctgcatc
     2041 ttctccaccg agttcgccct cgggatgatg ggcgacatcc agcaatggtt cgtcgacaac
     2101 gacgtgcgga acttctactc ggtgtcgatc agcggctatc acatggccga ggccggggcc
     2161 aacccgatca cccagctcgc gttcacgctg gccaacgggt tcacgtatgt cgagaactac
     2221 ctggcccgcg gcatggacat cgacgacttc gcccccaact tgtccttctt cttctccaac
     2281 ggcatcgacg ccgagtacgc agtcatcggg cgtgtcgctc gtcgcatctg ggccaaggcg
     2341 atcaaacacc gttacggagg caacgaacgg tctcagaagc tgaaatacca cgtgcagacc
     2401 tcgggtaggt cgcttcatgc ccaggagatc tccttcaacg acatcaggac cactcttcaa
     2461 gctctctacg ccatctacga caacaccaac agcctccata ccaacgccta cgacgaggcg
     2521 atcaccactc cgaccgagga atcggtgcga cgggcccttg ccatccagct gatcatcaac
     2581 caggagctgg ggctggcgaa gaaccagaac cccctccagg gaagcttcat catcgaggag
     2641 ctgaccgatc tggtcgaaga ggcggtgctt gccgagttcg accggatcaa cagccgcggg
     2701 ggagtgctgg gagcgatgga gcggcaatac cagcgcacca agatccagga ggaatcgctc
     2761 tactacgagc agcagaagga ggccggcgcc taccccgttg tcggggtcaa caccttcatc
     2821 tccaaagagg gctcgccctt tgtcatccct cgaaccctga tccggtccac cgacgaggac
     2881 aaggatcgcc agatcgccaa tgtgtgcgcc taccaggagc ggaatgccga gaccggcccc
     2941 caagccctcg aggagctgca gaaggtggcc gtcaccggag gcaacatctt cgcccaactc
     3001 atggagacgg ccaaggttgc ctccctgggg cagatgagca acgccctcta cggggtgggt
     3061 gggcagtacc ggcgcaacat gtgatcacat cggcagtggc ggctgatgga ttccgaacgg
     3121 tgacccctgg tcgtccctgc acagcttgaa ccgaccgaaa cgggcgatcg attcctcgtc
     3181 tccctccacg tccacatcgt cgaccgagcc acccaactcc tggatcttct cgacggcggc
     3241 ttctatgtct tcgacccgga agaagagata cggggcggcc cccggatcgc tcccatggat
     3301 gccggcgccg acattgggca aggagatcac gtagccctga cccgtcggcc cgggctcgaa
     3361 cttccaaccg aacaggcccc tgtagaaggc ccgtgccttg tcgacgtcct cgacaccgaa
     3421 ctcgacgaaa gccaattcgc ccgacaatcg ctctcctttc
//
LOCUS       DASTXO010000220         2986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1523953, whole genome shotgun sequence.
ACCESSION   DASTXO010000220 DASTXO010000000
VERSION     DASTXO010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523953"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VFU96_04630"
     CDS             <1..891
                     /locus_tag="VFU96_04630"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5112580.1"
                     /translation="MHELNNPAAAVGRSMARAEEVYVALGDATVLLSGIGELLARPIP
                     VPEKPELTDPLTRSSLEDGVATWLEDLEVDDAWDIAPALVADGWDIDLLQETTDGLDR
                     ETTTKFMRWLGLRVLASQLINEVRIAAGRISELVRVVKNYSYLDQGPVQMIDPTEGIR
                     DTLILLKHKLRGIETVVDIEPELPSIEASGRDLNQVWTNLIDNAADAMDGSGTLKIEA
                     ARENGAVVVRISDTGPGIDPEVQQRIFDPFFTTKAPGQGTGLGLHTVHTLVTRIGAEI
                     SVDSDSSGTTFTLNFPSAGE"
     gene            complement(897..1751)
                     /locus_tag="VFU96_04635"
     CDS             complement(897..1751)
                     /locus_tag="VFU96_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5112581.1"
                     /translation="MRAVVAGVDLTAMGRRVAERAGMMAQSSGVPLRLLHVLEPVAEA
                     MIEPSLARLMREYQTAEATKLAAWTRDKTGVEVELEVVKGSPSWELTARGKGAGLVVV
                     GSSSINAFSVGPVAKRVARKTTTDTLLVRRQPRVPYRKVIAAVDFSEHSRMAVNRALS
                     LFPEADTTALYSMPSRFDSMLASAGLFNEELEASRGSRLRMAEERMKEFAAAWDGDIR
                     TLVVDGPPTETIEEVVRRRGADLVIVASRGASATRMVLLGTVAEGLVAEAPCDVLVAR
                     AKAPFRRP"
     gene            complement(1792..2469)
                     /gene="aat"
                     /locus_tag="VFU96_04640"
     CDS             complement(1792..2469)
                     /gene="aat"
                     /locus_tag="VFU96_04640"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEU5112582.1"
                     /translation="MPIEPMPSRFDLPDPSPGDGDVVAVGADLDPGTLLQAYRKGLFP
                     MGLPDGHLGWWSPVDRAILPVRGLHISRSLRQSTRRFQVSVDADFEGVIRGCADSRRR
                     DPWITDDFVAAYTMLHRLGWAHSVEVWDNEGSLVGGLYGVAIGGLFAGESMFHEVRDA
                     SKVALVHLVVVMNRGGGRLLDVQWQTPHLESLGAVVIGRDAYLDKLKEALEGPEVFDA
                     AHESDSL"
     gene            2518..>2986
                     /locus_tag="VFU96_04645"
     CDS             2518..>2986
                     /locus_tag="VFU96_04645"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU5112583.1"
                     /translation="MRALICDGYDGLDALRIGDMPDPTPPPGTVSVAVKAAAVNFQDL
                     LVVAGQYQIKPDLPFVPGHEFAGVVEAANGVDDFAPGDRVVGFVGTGAMAEHAVADPG
                     AITRLPDHIGYEVGATIPVAYGTSYHALVDRAHLRADETLLVLGAAGGVGIAAV"
BASE COUNT          533 a          999 c          948 g          506 t
ORIGIN      
        1 atgcacgagc tgaacaaccc ggcagctgcc gtcgggcgga gcatggcccg tgccgaggag
       61 gtctacgtgg ctctggggga cgccacggtt ctgctctcgg ggataggcga gctcttggcg
      121 cggccgatcc cggtcccgga aaagcccgag ttgaccgatc ccctcacccg gtcatcgttg
      181 gaagacgggg tggcgacgtg gctggaagac ctcgaagtcg acgacgcatg ggacatcgcc
      241 cccgctctgg tcgccgacgg atgggacatc gacctgctcc aggagaccac cgatgggctc
      301 gaccgcgaga ccacgacgaa gttcatgcgc tggctggggc tgcgcgtgct cgcctcccag
      361 ttgatcaacg aggttcggat cgccgccggc cgaatctctg agctggtgcg cgtagtgaag
      421 aactactcat atctcgatca gggaccggtg cagatgatcg atcccaccga agggataagg
      481 gacactctga tcctgctcaa gcacaagttg cggggcatcg agaccgtggt cgacatcgaa
      541 ccggaactgc cttcgatcga agcgtcgggg cgagacctca accaggtttg gaccaacctc
      601 atcgacaatg ccgccgacgc gatggacggc tcgggaacct tgaagatcga ggcggcacgc
      661 gagaacggag ctgtagtagt ccgtatctcg gacaccggcc cggggatcga tcccgaagtc
      721 cagcagagga tcttcgaccc gttcttcacc accaaggcgc cagggcaggg aaccggcttg
      781 ggccttcaca cggtgcacac cctcgtgacc cggatcggcg cagagatctc ggtcgattcc
      841 gactcgtcgg gcaccacgtt cactctcaac ttcccatcgg caggcgaata gacgactcag
      901 gggcgccgga atggcgcctt ggctcgggcc accagcacat cacagggagc ctcggcgacg
      961 agcccctctg ccactgtccc cagcaggacc atcctggtgg cgctggctcc ccgactcgcc
     1021 acgatcacca ggtccgcgcc tcttctcctc accacctcct cgatggtctc ggtgggaggg
     1081 ccgtcgacga ccagagtcct gatgtcgcca tcccatgcag ccgcgaactc cttcatgcgc
     1141 tcctcggcca tgcgcaggcg tgagccacgt gaggcctcga gctcctcgtt gaagagcccg
     1201 gcgctggcga gcatcgagtc gaatcgggag ggcatcgagt acagagccgt ggtgtcggcc
     1261 tcggggaaga gtgacagcgc ccggttcacc gccatccgcg agtgctcaga gaaatccacg
     1321 gcagcgatca cctttcgata gggcactcgg ggctgcctcc gcaccaggag tgtgtccgtg
     1381 gtggtcttgc gggccactcg ttttgccacc gggcctaccg agaaggcgtt gatggaggat
     1441 gatccgacca ccaccagccc cgcccccttc cctctcgccg tcagttccca tgacggagaa
     1501 cccttgacga cctccagctc gacctccacc ccggtcttgt ccctggtcca tgcagccaac
     1561 ttggtggcct ccgcggtctg gtactcgcgc atcagacggg ccaggctggg ctcgatcatc
     1621 gcctcggcga ccggctccaa gacgtgaaga agtcgcagcg gcacaccgga tgactgggcc
     1681 atcattccgg cccgctcggc tacgcgccgc cccatggcgg tcagatcgac tccggcaacg
     1741 acggcccgca acgctcctcc aggaatgggc ctcgactcta gtggcggggt ctcagaggct
     1801 gtcactctcg tgagcagcgt cgaagacctc gggcccttcc agggcttcct tcagcttgtc
     1861 gaggtaggcg tcacgaccga tcacgaccgc cccgagcgac tcgaggtggg gtgtttgcca
     1921 ctggacgtcg agcaagcggc cccctccgcg attcatcacc accaccaggt ggaccagggc
     1981 cactttggac gcatcccgca cttcgtggaa catcgattcg ccggcgaaga gcccaccgat
     2041 cgcgactccg tagagaccgc ccacaagact gccctcgttg tcccacacct cgaccgaatg
     2101 ggcccacccg aggcggtgca gcatcgtgta cgccgcgacg aagtcgtcgg ttatccaggg
     2161 atctctccgt ctcgaatcgg cgcagccccg gatcactcct tcgaaatcgg catcgaccga
     2221 gacctggaag cgacgtgtcg actgacggag cgatcggctg atgtgcaggc ctcgcacggg
     2281 aagaatcgcc cgatcgaccg gcgaccacca accgagatgc ccgtcgggca ggcccatggg
     2341 gaacaggccc tttcgatagg cctggagcag ggtgccggga tccaggtcgg cgcccacggc
     2401 cacgacgtca ccgtctcccg ggctggggtc gggaaggtcg aacctggacg gcattggctc
     2461 gatcggcact ccccgattgt gtcagagagg gacaagcgat gtccgtaggc ttgatcgatg
     2521 cgcgccctga tctgtgacgg ctacgacggt ctggacgccc ttcggattgg cgatatgcca
     2581 gatccgacgc cacccccagg gacggtatcg gtcgcggtca aggcggcggc agtcaacttc
     2641 caggacctct tggtcgttgc cggtcagtat cagatcaagc cagatctgcc cttcgttccg
     2701 ggtcacgaat tcgccggagt ggtcgaggcg gccaacggcg tcgatgactt cgcccctggc
     2761 gaccgggtgg tcgggttcgt ggggacgggt gccatggcgg agcacgccgt cgccgatcct
     2821 ggtgcaatca ctcgtcttcc cgatcacatc ggctacgagg ttggggccac gatccctgtc
     2881 gcctatggga cctcctatca cgccttggtc gaccgggcac acctcagggc ggacgagaca
     2941 ctgctggtgt tgggggcggc cgggggtgtc ggcatcgccg ccgtcc
//
LOCUS       DASTXO010000221         2796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_566453, whole genome shotgun sequence.
ACCESSION   DASTXO010000221 DASTXO010000000
VERSION     DASTXO010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566453"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="VFU96_04650"
     CDS             <1..964
                     /locus_tag="VFU96_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5112584.1"
                     /translation="PCILENGCEGASFPEFDPEAARALLAEAGFPDGFPTTITLRDVF
                     RVYLPEPTAVAEDIRAQLAENLGINATIEVMESGAFIEATAAGQVEGIHLLGWGADYP
                     HVTNFLDYHFGGINVQFGVMNQEIADAVIEGSGIVDPAEAQPVYAQINDLLVEDVPMV
                     PIAWGAAANAGLATLEGAVTPPFGAPQFALMNPGDSDLVFVQNAEPISLYCPDETDGE
                     SLNGCQMTQEGLYGYDLEGQAVPKLATECVPDEAGVVWTCALREGVTFHDGSAFDAND
                     VVASWSASLDAANPLHTGNTGVFEYPATLWNGLMNPPPTTTAAG"
     gene            1040..2119
                     /locus_tag="VFU96_04655"
     CDS             1040..2119
                     /locus_tag="VFU96_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012653730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5112585.1"
                     /translation="MIGYILRRILVAIPVLIGVLLLVFLLVRLLPGEPCTAILGERAT
                     AEACERFNEANGLDEPILVQLGIYFGNVISGDLGESIRFSRPVTQIIIERLPVTLELT
                     IAALSVAIIVGIPLGIAAARRHNSSVDVAAMTFANVGVSMPIFWLGLLLAYLFAVVLS
                     GTIFALPPSGSLSPGNRALPFYEVWGWEISEEGFVSTAAEFVSYMTVTNALISAQWAV
                     FWDGFKHLILPAITLATIPMAIIARMTRSSLLDVLGRDYIRTARAKGAMERKVVRGHA
                     LRNAMLPVVTIIGLQLGLLLGGAVLTETVFGLAGVGTALFDAITARDFPIIQGFTLII
                     ATTYVVVNLLVDVSYGYLDPRVRLE"
     gene            2116..>2796
                     /locus_tag="VFU96_04660"
     CDS             2116..>2796
                     /locus_tag="VFU96_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5112586.1"
                     /translation="MTLGEAEMNFRPDVALPDPESLLEQPGSVWRIAGRRFFQRKVGI
                     IGLILVCFMILVAIFAPLIAPYDPYQNFIGQPGAERWLPPCINAFGCEGTQHIFGLDG
                     NVRDFFSRVVYAARVSLPLGFTTVLITIVIGTVIGAIAGYAGGWSDNVMMRIMDVVLG
                     FPSLILAIAIVFVLGPGLRNTLLAIAIVTIPQYARVMRASVLSIKEVDYVSASRALGA
                     SRWKVLVTR"
BASE COUNT          482 a          905 c          855 g          554 t
ORIGIN      
        1 gccttgcatc ctcgagaacg gctgcgaggg cgcatcgttc cccgagttcg atcccgaggc
       61 ggcacgggcg ctgctcgccg aagccggatt ccccgacggg ttccccacaa cgatcacgct
      121 ccgtgatgtg ttccgggtct atctcccgga gccgacggcg gtggccgagg acatccgcgc
      181 ccaattggcg gagaacctgg ggatcaacgc cacaatcgag gtcatggaat ctggcgcgtt
      241 catcgaggcc acggccgccg gccaggtgga aggcatccac ctgctgggct ggggcgccga
      301 ctacccacat gtgaccaact tcctcgacta tcacttcggt ggcatcaatg tccagttcgg
      361 agtcatgaac caggagatcg ccgacgcagt gatcgaagga tcggggatcg tcgatccagc
      421 cgaggcacag cccgtctacg cgcagatcaa cgatctgctg gtggaggacg tgccaatggt
      481 tccgatcgct tggggagccg cggcgaacgc cgggctcgcc accctcgaag gtgcggtgac
      541 gccgccattc ggtgcgccac agttcgcgct catgaatccg ggcgattccg acctggtgtt
      601 cgtccagaac gccgagccga tctcgctcta ttgccccgac gagaccgatg gcgagtcatt
      661 gaatggatgt cagatgacgc aggaaggcct ctacggatac gacctggaag gtcaggccgt
      721 gccgaagctg gccaccgagt gtgtgcctga cgaagctggc gtggtatgga cctgcgccct
      781 gcgcgaaggt gtcaccttcc acgacggctc tgcgttcgat gccaacgacg tggttgcctc
      841 ttggtcggcc tcgctcgacg cagcgaatcc actacacacc ggtaacaccg gggtgttcga
      901 atatcccgcg acgctgtgga acgggttgat gaacccaccg cccacgacga cagcggctgg
      961 atgagagtct gatcgctcag acagatgacg gggggaggcg cgacagcgcc tccccccgtt
     1021 ctacgctccg gttgaggaaa tgatcggata catcttgcgt cgcatcctgg tcgcgatacc
     1081 tgtcctgatc ggggtcctgc tcctcgtatt cctgctggtc cgactgctcc cgggggaacc
     1141 gtgcaccgcc atcctcggcg agagggcgac cgcggaagcg tgcgaacgat tcaacgaggc
     1201 caacggcctc gacgagccga tcctcgtcca actcggcatc tacttcggga acgtgatcag
     1261 cggtgatctc ggagagtcga tccgtttctc ccggccggtc acccaaataa tcatcgagcg
     1321 gcttcccgtg acgctcgagc tcaccattgc cgcgttgagt gtggcgatca tcgtcggcat
     1381 cccactcggg atcgccgcag cgaggcgcca caactccagc gtcgacgtcg ctgcgatgac
     1441 ttttgccaat gtcggcgtgt cgatgcctat cttctggctc ggcctcctgc tggcctatct
     1501 gttcgccgtc gtgttgtcgg ggacgatctt cgccttgcca cccagcggat cactcagccc
     1561 ggggaaccgc gccctcccgt tctacgaggt gtggggttgg gagatctccg aggagggatt
     1621 cgtctcgact gccgcggaat tcgtctccta catgactgtg accaatgccc tcatcagcgc
     1681 ccagtgggcg gttttctggg acgggttcaa gcatctcatc cttcccgcca tcacgctggc
     1741 cacgattccg atggcgatca tcgcccgaat gacccggtca tcgctcctcg acgtgctggg
     1801 ccgcgactac atccgcaccg ctcgggccaa gggcgcgatg gaacggaaag tcgtgagggg
     1861 ccacgcacta cgcaatgcaa tgctcccggt ggtcacgatc atcgggctcc agctgggcct
     1921 gctcctcggc ggtgccgtgc tgaccgagac cgttttcggc ctggccggtg tcggcactgc
     1981 tctgttcgac gcaatcaccg cacgggactt tcccatcatc cagggattca cgctgatcat
     2041 cgcaactacg tatgtggtcg tgaacctgtt ggttgacgtc tcctatggct acctggaccc
     2101 gagagtgagg ctggaatgac acttggtgag gctgagatga attttcggcc ggacgtggcc
     2161 ctccccgacc cggagtcgct tctcgagcaa ccaggctcgg tctggcgcat cgccggtcgc
     2221 cgcttcttcc agcgcaaggt cgggatcatc gggctgatcc tcgtctgctt catgatcctg
     2281 gtggcgatct tcgccccgct catcgccccg tacgacccgt accagaactt catcgggcag
     2341 cccggagcgg aaaggtggct gcctccatgc atcaatgcat tcgggtgcga agggacacag
     2401 cacatcttcg ggctggacgg aaacgtcagg gatttcttca gccgggtcgt ctacgcggcc
     2461 agggtgagcc tgcccctcgg cttcacgacc gtcctcatca cgatcgtcat cgggaccgtg
     2521 atcggggcaa tcgccggcta cgcaggaggg tggagtgaca acgtgatgat gcggatcatg
     2581 gatgtcgtgc tcggtttccc gtccctcatc ctcgccatcg ccatcgtctt cgtcctcggg
     2641 ccaggactcc gtaacactct gctcgccatc gccatcgtca ccatccccca gtacgcacgg
     2701 gtgatgaggg cgtcggtgct ctccatcaag gaggtcgact acgtatccgc atctcgggct
     2761 cttggcgcgt cccgatggaa agtgctcgtc acccgg
//
LOCUS       DASTXO010000222         2852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_13872, whole genome shotgun sequence.
ACCESSION   DASTXO010000222 DASTXO010000000
VERSION     DASTXO010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13872"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2526
                     /locus_tag="VFU96_04665"
     CDS             <1..2526
                     /locus_tag="VFU96_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5112587.1"
                     /translation="DYPELLRMYRETTEGVVARRGGAIFGSEGDGLFAAFAEAGEAVS
                     SAIDCQDGFRSAAWPAGAQVRVRMGIHTGTPTLIGDDYTGIDVHRAARIMSAAWGGQV
                     LVSEATRSLITDTGIKCRELGLYAMKGLSRNERLYQLEGAGLNGDFPPVRARRHEVDL
                     PSPPTPLVGREEDIVTVERLLLDDMTRLVTLTGAGGIGKSRLALAVAAKVAAEFADGI
                     GYVDVSNETMPERVFTSIAEALGVAGDPSHPALDALIDHLADLDLLLVLDGFERVSAA
                     AVDLADLLTRCPDLRVLVTSRAPLRIGAEREIRVSPLEIPAAGSGFEAISASPAVRLF
                     VERAMAVRPDFRLDASNAAVICDLVARLEGYPLSIELASARARLLPPEAILERLGDVL
                     DLATTSPELPTRQQSLRATIEWSHGLLSEPDQALFRRLGVFVDGWSLEAAEAVAGGDE
                     PDVVLGLETLAAQSMISVDPDGRMSMGTAMRDFASERLAVAGDEEETRLRHAEHFDSA
                     AVAAEPLLRGPRQREIINALSKDWRNLRAAAEWALETDRIELAGSLYTSSWILAWQGD
                     HWNDTDKYTSKFVGVIDRFDEQLQARILFVAAGFRMETGDGPTAIAYARPAIELANRV
                     GDRETEAWAHLMLAGSLLYADASAPEPREHIDRAVALARSLDDPFVLGYSLSFQAAIS
                     TLDGDLATGLANHFEVLEIANRLEIVPLICQTYSQVAMTHLTAGDIGLARTALESAAE
                     YVDRLRSMESLAVLLDGVSWLAFAEGDQVRAMTALGAANAARSRVGLVRWALIAELLD
                     AAGFAAEAEHPGLADARHAGEEMSPQDAIAYALEPHHELAATG"
     gene            2530..>2852
                     /locus_tag="VFU96_04670"
     CDS             2530..>2852
                     /locus_tag="VFU96_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112588.1"
                     /translation="MNVDTSDLWTCPVCGKRFVTRNMSHSCGLHTVELFMEGKGPQAW
                     AYWDRLQEIVAICGPYSIVANKTRLEFMVRVRFAGMDAVSEQGMSFSFWLKESLDSPR
                     LRKVVL"
BASE COUNT          465 a          936 c          942 g          509 t
ORIGIN      
        1 gactacccgg agctcctgag gatgtaccgg gagaccactg agggagtggt ggcccggcga
       61 ggcggggcca tttttggcag cgaaggcgac ggtctctttg ctgctttcgc cgaggccggt
      121 gaggcggtct cgagcgccat cgactgccaa gacggtttcc gaagcgctgc atggccggcc
      181 ggggcacagg tgagggtgcg aatgggtatc cacaccggga ctcccacgct gatcggagac
      241 gactacacgg gcatcgacgt ccatcgggcg gcccgaatca tgtcggcggc ttgggggggc
      301 caggtgctcg tttcggaggc gacaagatcc ctgatcaccg acaccggcat caagtgcagg
      361 gagctcgggc tgtacgcgat gaagggcctg agtcggaacg agcgtctcta ccagttggaa
      421 ggagccggac tcaacggaga cttccccccg gtgcgagcgc gccgtcatga ggtcgatttg
      481 cccagccctc ccacgcccct ggtcggccgc gaggaggaca tcgtgacggt ggagcggctc
      541 cttctcgatg acatgacgcg tctggtgacc ctcaccggag cgggcggtat cggcaagagt
      601 cgtctggccc ttgccgtggc ggcgaaggtc gctgccgagt tcgccgacgg catcggatat
      661 gtggacgtgt cgaacgagac gatgcccgaa cgggtcttca cctcgatcgc tgaggcgctc
      721 ggagtggccg gtgacccgag tcatccagca ctagatgccc tcatcgacca tctcgccgat
      781 ctagatctgc ttctcgtgct cgatggattc gagcgggtca gtgcggctgc ggtcgacctc
      841 gccgatctcc tcaccaggtg cccggacctg cgggtcctgg tcaccagcag ggctccactc
      901 cgtatcggtg ccgaacgtga gatccgagtt tctccgctcg agattccggc tgccggttcg
      961 ggattcgagg ccatctctgc ctcccctgcg gtccgtctct tcgtggaacg ggcgatggcc
     1021 gtgcgccctg atttccgact cgacgcttcc aacgccgctg tcatctgtga cctggtggca
     1081 cgcctggagg gctacccgct ctcgatcgag ttggcctcgg ctcgggcgcg actgctcccc
     1141 ccggaggcga tcctggaacg gctgggggac gtcctcgatc tcgccaccac ttccccggag
     1201 cttcccacca ggcagcagag cctgcgtgcc acgatcgaat ggagtcacgg gcttctctcc
     1261 gagcccgatc aggccctgtt ccgtcggctg ggtgttttcg tcgacggctg gtcgctggaa
     1321 gccgccgagg ccgtggctgg aggcgacgaa ccggacgtgg tccttggtct ggagactctc
     1381 gccgcacaga gcatgatctc cgtcgacccc gacggccgga tgagcatggg cacggcgatg
     1441 cgcgatttcg cgtcggagcg cctcgccgtg gccggggacg aggaggagac ccggctccgt
     1501 cacgcggaac acttcgactc cgcagctgtc gccgccgagc ccttgcttcg tgggccgcga
     1561 cagcgagaga tcatcaacgc gctgtccaag gattggcgca acctgcgggc cgcggccgaa
     1621 tgggcgctcg agaccgatcg aatcgagctg gcgggctcgc tctacaccag cagctggata
     1681 ctggcctggc agggtgatca ctggaacgac accgacaaat acaccagcaa gttcgtgggc
     1741 gtgatcgacc gattcgacga gcagcttcag gcgcggatcc tgttcgtcgc cgccggattc
     1801 cggatggaga ccggtgacgg tcccaccgcc atcgcctacg cccggcccgc catcgaactg
     1861 gcgaaccgag tcggagaccg agagaccgaa gcctgggcgc atttgatgct ggccggctcg
     1921 cttctctacg cggatgcctc cgcccccgag ccccgagagc acatcgaccg ggcggtggcc
     1981 ctggcccgtt cactggacga tcctttcgtg ctcggatact ccctcagctt tcaggcggcc
     2041 atctcgaccc tggatgggga tctggctacc ggcctcgcca accacttcga agtgctggaa
     2101 atcgccaatc gtctggagat cgtccccctg atctgccaga cctacagcca ggtcgcgatg
     2161 acacatctga ctgccggaga tatcggtctt gcgcgcaccg ccctcgagtc cgcggccgag
     2221 tacgtagacc gtctccggag catggagtcc ttggcggtgc tgctcgatgg ggtctcctgg
     2281 ctcgcgttcg ccgagggcga ccaggtcagg gcgatgacag ccctgggggc ggccaacgcg
     2341 gcccgatcac gtgtcgggct cgtcagatgg gcgctgatcg cagaactgct cgacgcggcc
     2401 ggtttcgccg cggaagccga acacccgggt ctcgccgacg cccgccacgc cggggaggag
     2461 atgtcccctc aggatgccat cgcctatgcc ctggagcccc accacgagct ggccgctacc
     2521 ggctgaggaa tgaacgtcga cacgtccgat ctgtggacct gtcccgtctg tgggaagcgg
     2581 ttcgtcaccc gcaacatgtc ccactcctgc gggctccaca ccgttgagtt gttcatggag
     2641 ggcaagggac cccaggcctg ggcctactgg gatcgactcc aagagatcgt ggcgatctgc
     2701 ggcccgtatt ccatcgtcgc caacaagacc cggctcgaat tcatggtgcg agtccgcttc
     2761 gccggcatgg acgcggtctc ggagcagggg atgagcttct cgttctggct caaagagagt
     2821 ctcgatagcc cacggctccg caaggtggtc ct
//
LOCUS       DASTXO010000223         4173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1303280, whole genome shotgun sequence.
ACCESSION   DASTXO010000223 DASTXO010000000
VERSION     DASTXO010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303280"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VFU96_04675"
     CDS             complement(<1..653)
                     /locus_tag="VFU96_04675"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018351056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEU5112589.1"
                     /translation="MTHIGVLTAGGDCPGLNAAIRGVVARATADGHPVTGIEDGWQGL
                     MGGRVRPLGRDDVRGILTRGGTILGTSRTDPYEHGVGLASLMPALAASGIDSVVVIGG
                     DGSLRTAARLAEEGLSVVGVPKTIDNDIEGTEVSFGFDTAVQIVTDAIDRLTTTAEAH
                     NRIMVVEVMGRQSGWIAVHAGLAGGAEAVLVPEENYDLDDLARRLMARHQGGRDYSIV
                     "
     gene            complement(713..3025)
                     /locus_tag="VFU96_04680"
     CDS             complement(713..3025)
                     /locus_tag="VFU96_04680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEU5112590.1"
                     /translation="MRRPSDVFKAVLGLLLVIWAAVNVHSISSWAQALTELVQSSPSW
                     VILLLEIGFTASLIYVAVVFVALITGGRERRPALRDLAIAGLTAGVLVVILSFIINGA
                     WPYVFPEIGLEDPTPRFPVLRVAMVTAILVVVSPHVTRPLRRFGWLAIAATSIASISL
                     GYGTPTHIIGSFGIGLFSAGLLLAIVGSPQGYPDPKSVAAALSSLGVLVNALEPAPYQ
                     TWGVIRFVGRDADDEIVDVKVHGRDAVESRLVAKLWHTLWYRESGMTLGYSRLQAVEH
                     EALITIVADRAGVRVPQLAAVGSPTSEVSLISFRGIGTALPEMDAGDLTDDLLVQIWH
                     QVRLMQEGALSHGSLDTSAVQVGSDGPIITDFALGSLSADDSDQATDIVELLFSLAVL
                     VEEERAVRTAIQGLGRERLVSVLPYLQVPAVSPTTRRLTEKPKDLMSSLASKVTEVTE
                     VEMPEPVKLRRVTVRNLIMAALLLLVAWAVIPLFTSVDYAEIWAVLESADWPLLVLAL
                     IVGQSQFFPQATATMCAVQVKLPFWPLLTLQTASQFISLAIPSAAGRVAMNTAFLTKF
                     GLTVPVALVQGSIDGFSGFLVQAAILILVLLFGDVDLGLDIDPADVPWLLILGLVALI
                     GIGLVVAVMRIKALRDRVGPIVKEAWGALGVVLRQPSRAFGLLGSNFVYWNVLGITLW
                     LTLQAVGSGLNYGSALFVAAGTSLLAGFMPVPGGVGVAEATMTALLVTFGVDQSTAFA
                     VTVAYRLITFYLPALEGFFGARWLGKHGYV"
     gene            complement(3255..3800)
                     /locus_tag="VFU96_04685"
     CDS             complement(3255..3800)
                     /locus_tag="VFU96_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112591.1"
                     /translation="MTIGPIQGLVIGFADNEMFEGRIAEELARLSDVGQIRVIDAVFV
                     TRTGDEIDVLSVSGLDDEQRAELRAVVGALVGLGVAGEEGAVAMAEAGATVDVDAMTV
                     AESVAAGLVDELPDGSSALVLAFEHLWAVPLRDAVRDAGGVVLAHRTLSAEDLIGLGM
                     LMGYAAEIEAEQEAAEASLDT"
     gene            complement(3931..>4173)
                     /locus_tag="VFU96_04690"
     CDS             complement(3931..>4173)
                     /locus_tag="VFU96_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112592.1"
                     /translation="VSADQAASGASAGDSLAAAVGAAAVFLTITSAAGILLAWLAGRH
                     RPPAPLAVDYAAAAAAGSHTLPAPHPSSAERVGSGV"
BASE COUNT          851 a         1386 c         1277 g          659 t
ORIGIN      
        1 acaattgaat agtccctacc accctggtga cgagccatca gccggcgcgc gaggtcgtcg
       61 aggtcgtagt tctcctcagg aacgagaacc gcttccgccc cgccggccag cccggcatgc
      121 accgcgatcc accccgactg ccgtcccatg acctccacca ccattatccg gttgtgcgcc
      181 tccgcggtgg tggtgaggcg gtcgatggca tcggtgacga tttggaccgc agtgtcgaag
      241 ccgaacgaca cttccgtgcc ttcgatgtca ttgtcgatgg tcttgggcac cccgacgacc
      301 gaaaggcctt cctctgccag cctggccgcg gtccgcagcg agccgtcgcc gccgatcaca
      361 acgacggagt cgatacccga agctgccaag gccggcatca gcgaggcgag accgacaccg
      421 tgctcatacg ggtcggttcg cgacgttccc aggatcgtgc cgccacgggt gagaattcca
      481 cgcacatcgt ctcgtcccag tggcctcacc cggcccccca tcaagccctg ccacccatct
      541 tcgattccgg tcacggggtg accatctgcc gtggctcgcg cgaccacacc gcggatggca
      601 gcattgagcc cggggcaatc cccaccagcg gtcagcacgc ctatgtgtgt cacgaattgt
      661 gattgtgcca gcgtcgcatc cggccagatg tccttcagac gctcgggggt gtctacacgt
      721 aaccgtgttt ccccagccag cgtgccccga agaagccttc cagcgccggc aggtagaaag
      781 tgatcaaccg ataggccacg gtgacggcga aggccgtcga ctgatcgaca ccgaatgtga
      841 ccaacaaagc ggtcatcgtc gcctcggcca cgccgacgcc tcccggcacc ggcatgaacc
      901 cggctaggag actcgtgccc gcggcgacga acagtgctga gccgtagttc aagccgctcc
      961 cgaccgcctg caaggtcagc cacaacgtga tcccgagcac gttccagtag acgaagttgc
     1021 tccccaacag cccgaaagcg cgcgacggtt gtcgaagcac gacccccaag gctccccacg
     1081 cctctttgac aatggggcct acgcggtctc gaagcgcctt gatcctcata accgcgacca
     1141 ccagtccgat cccgatcaga gccaccaggc cgagtatgag caaccacggc acgtcggcag
     1201 gatcgatgtc gagaccgagg tcgacgtcgc cgaagagcag gacgaggatg aggatggccg
     1261 cttggaccag gaacccggag aagccgtcga tggagccttg gaccagggcg accggaaccg
     1321 tcagaccgaa cttggtgaga aacgcggtgt tcatcgcgac acgtccggct gcgctcggga
     1381 tggccaggct gatgaactgg ctcgccgtct gcaatgtcag caagggccaa aacggcagct
     1441 tgacctggac ggcgcacatc gttgccgtgg cctggggaaa gaattgagac tgcccgacga
     1501 tcaaggcgag gacgagcagg ggccaatccg ccgactcgag gacagcccag atctcggcgt
     1561 aatcgacact ggtgaacagc ggaatcacgg cccacgcgac caagaggagg agcgccgcca
     1621 tgatgagatt tctcactgtg acccggcgga gcttcaccgg ctccggcatc tcgacttcgg
     1681 tcacctcggt caccttcgag gccagcgatg acatcaggtc ctttggcttc tcggtgagac
     1741 gccgggtagt cgggctgacc gcaggaactt ggagataggg caggacggaa acgagtcgtt
     1801 cccgccccag accctgtatt gccgtgcgga ctgcccgctc ttcctcgacc aggacagcga
     1861 gcgagaacag aagctcgacg atgtcggtgg cctgatcgga gtcatccgca gatagagagc
     1921 caagggcgaa atcggtgatg atcggcccat ccgagcccac ctggacagcc gaggtatcca
     1981 gtgagccatg gctcaacgct ccctcctgca tgagacgcac ctggtgccag atctggacga
     2041 ggaggtcgtc ggtgaggtcg cctgcgtcca tctccggaag agcggttccg ataccgcgga
     2101 acgagatgag ggatacctcg gatgtggggc tccccactgc ggccagttgt ggcacccgca
     2161 ccccggcgcg atcggcaacg atggtgatca aggcctcgtg ctccaccgcc tgaagccgtg
     2221 agtagccgag cgtcatgccc gattcgcggt accagagggt gtgccagagc tttgccacga
     2281 gccgtgactc gaccgcgtcg cgtccgtgca ccttgacgtc gacaatctcg tcgtccgcgt
     2341 cccgaccaac gaaacgaatc actccccacg tttggtaggg cgccggctcg agagcgttga
     2401 cgagaacacc gaggctggag agagcggcgg ccacggactt gggatcgggg tacccctgag
     2461 gcgaccccac gatggccaag agaaggccag ccgaaaagag accgatcccg aaagagccga
     2521 tgatgtgggt cggggtccca tatcccagac ttatcgatgc gatggatgtg gccgcgatgg
     2581 caagccagcc gaagcgtcgg agagggcgcg tgacgtgggg actgaccacc acgaggatgg
     2641 cggtcaccat cgccactctc agcacgggaa accgcggcgt gggatcctcc aacccgatct
     2701 cggggaatac gtatggccag gctccgttga tgatgaacga gagaatcacc accagcaccc
     2761 cagcggtcag gcctgcgatc gccagatcac gcaatgcagg tcgccgctcc cgacctcctg
     2821 tgatcaacgc gacgaagacg acagctacgt agatgaggct ggctgtgaag ccgatctcga
     2881 ggaggaggat cacccaggat gggctcgact gcaccagctc ggtgagagcc tgtgcccagg
     2941 acgagatgct gtggacgttg accgcggccc aaatgaccaa gaggaggcca agaacagcct
     3001 tgaacacgtc gctcggccgc cgcacccggg gctggctccc ggacgtgaca aaatacgagg
     3061 aaggcccaac agtgagttgt ttgatcgcgt ctctcccggg tctcgtcaga tcctgatctt
     3121 gcaagctacg tcggaatcgg cctgcaatgt gtcatccgat atggccgacc gaggctgaca
     3181 ccgcgaagga tcctctaccc ggctcgaatt tggatatcgg gtcgtcgatg aggtcaacac
     3241 ctcatcgacg tcattcaggt gtcgagcgag gcttcggcgg cttcctgttc cgcttcgatc
     3301 tcggctgcgt aacccatcag cattcccaac ccgatcaagt cctccgccga aagagtgcgg
     3361 tgggcgagga cgacaccccc ggcatcgcgg accgcgtctc gcaacggcac cgcccagagg
     3421 tgctcgaagg ccaggaccaa ggcggaactg ccgtcaggca gttcgtcgac caagccggcg
     3481 gcaaccgact cagcaacggt catggcatcg acgtcgacgg tggctcccgc ctcggccatc
     3541 gctaccgctc cttcctcgcc agcgactccg agtccgacca gggcaccgac caccgcgcgc
     3601 agctcggccc tctgctcatc gtcgagacca gagaccgaca acacatcgat ctcatcgccg
     3661 gtgcgggtca cgaaaacagc atcgatgacc cgaatctgcc cgacatcact gagacgtgcc
     3721 aactcctcag cgatccgacc ctcgaacatc tcattgtccg cgaaaccgat caccaacccc
     3781 tgaataggtc cgatggtcat cctgacgtac ctcctgctcg acatccgccg cctgattgac
     3841 ggcactcttc gttgttggcc acagagccat ctctcgccac gcccggatcg aaccatacgc
     3901 tttccatgtg cggctcgacc tcgagccgag ctataccccc gacccgaccc tctcggccga
     3961 ggatgggtgc ggagcgggca gggtgtggct gccggcggcc gcagcggcgg cgtaatcgac
     4021 agcgagcggt gccggcggcc ggtgccgacc ggccaaccag gccaggagta tcccggccgc
     4081 agatgtgatg gtcaggaaca cggcagccgc tccgaccgcc gccgccagac tgtcgccggc
     4141 agaggcgccg gatgcagcct ggtcagcgct gac
//
LOCUS       DASTXO010000224         3096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1634764, whole genome shotgun sequence.
ACCESSION   DASTXO010000224 DASTXO010000000
VERSION     DASTXO010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1634764"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VFU96_04695"
     CDS             complement(<1..621)
                     /locus_tag="VFU96_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEU5112593.1"
                     /translation="MPNRLAESTSPYLLQHKDNPVEWHEWGDEAFSEAHRRDVPVLLS
                     VGYAACHWCHVMAHESFEDEETAAEMNRLYVNVKVDREERPDVDSIYMEAVQTMTGQG
                     GWPMTVWLAPDGRPFYAGTYFPDTDRHGMPSFRTVMRAVSDAWENRREEVLGQSSRLT
                     EAISRQIPRAEDDPGEEILARGYESLAASFDPVNGGFGSAPKFPQAP"
     gene            683..1336
                     /locus_tag="VFU96_04700"
     CDS             683..1336
                     /locus_tag="VFU96_04700"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEU5112594.1"
                     /translation="MTDQDIQALYLSAEQRWMQGWLAGPTIEGSPLHRGDRAPEVDVV
                     DEHGGTVSLATVWADQPGLVLLWRHLGCGCGLERGERLKEEHQAYVDSGLNVVIVAPA
                     DLQRVTLYKERYQIPVTMYSDPGYTAHKAFGLGHWALEQALYDAPDEYCELSEETGRQ
                     FQEDRRSQGRRLVDDPWMQSGEFVVAPDGRIRVAYLYNYCADYPDPRVFTTAGRLLG"
     gene            1348..1842
                     /locus_tag="VFU96_04705"
     CDS             1348..1842
                     /locus_tag="VFU96_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU5112595.1"
                     /translation="MKTLLLMRHAKSDWDADYGSDHDRPLNDRGQRSARLMGRVLADE
                     DLVPDLIISSTAVRARRTAELAIEAGAWDAELVLDRSLYDEGPRGVLESGASAPDVNR
                     VMLVGHQPTWSMLVSALTGERADLKTATVAVIELDLDSWAGLPGASGNLTRVLGPKTY
                     DLEP"
     gene            complement(1850..2206)
                     /locus_tag="VFU96_04710"
     CDS             complement(1850..2206)
                     /locus_tag="VFU96_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEU5112596.1"
                     /translation="MPSPTDVEEFLTNLPDDVGAALQHLRETIRRAAPDAEERIGYGV
                     PAFYYKNRPLVSYGAGKNHCAFYVQSPAVIETHRAELAGYDTAKGTVRFHADAPLPAT
                     LVEILVKARIAETDAT"
     gene            2446..>3096
                     /locus_tag="VFU96_04715"
     CDS             2446..>3096
                     /locus_tag="VFU96_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5112597.1"
                     /translation="MPAAETRTEKTEKPRIRLHSKLTDERGRLTTDLVSQYLTAIGEY
                     DLLTAEQEVELAQKIEAGEEAAEKLEKGEYNGKREEMELRRFARRGAQAKDDFLTANL
                     RLVVANARRYANTSGIDFLDLIQEGNLGLIRAVEKFDWRKGFKFSTYATWWIRQAITR
                     AIADKSRTVRIPVHLHDTLAAVRAAQASLKAELGRDPRPEEIAEEAGVTADKVELAL"
BASE COUNT          569 a          943 c         1022 g          562 t
ORIGIN      
        1 gggcgcctgg gggaacttcg gggcgctgcc gaacccaccg ttgactgggt cgaacgacgc
       61 ggccagtgac tcgtaaccac gagccaggat ctcttcccct gggtcgtcct cggcgcgggg
      121 tatttgccgg ctgatcgcct ccgtgagccg ggacgattgg cccaacacct cctcccgccg
      181 gttttcccac gcgtccgaca ctgcccgcat caccgtgcga aaggagggca tcccgtgtcg
      241 gtcggtgtcg gggaaatagg tgccggcgta gaaggggcgc ccatcggggg cgagccagac
      301 ggtcatcggc cagcctccct gaccggtcat ggtctggacc gcctccatgt agatgctgtc
      361 gacgtcgggt cgctcttcgc gatcgacctt gacattcaca tagagacgat tcatctcggc
      421 cgcggtttcc tcatcctcaa acgactcatg tgccatcacg tggcaccagt ggcaggcggc
      481 atagccaacg ctgagaagga cgggcacgtc ccgtcggtgg gcttcggaga acgcctcgtc
      541 cccccactcg tgccactcca ccgggttgtc cttgtgctga aggagatacg gagacgtgga
      601 ttcggcgagg cgattgggca tcgatgtcga acgtacacgc tcgcgattag ggtcgccgac
      661 gcggcgaatc gacggaggga atgtgaccga ccaggacatc caggcgctct atctgtctgc
      721 cgagcagcgc tggatgcaag gatggctcgc cgggccaacc atcgaaggca gcccactgca
      781 ccgcggagat agggcgcccg aagtggatgt ggtggacgag cacggtggca ccgtgtcact
      841 cgcaacggtc tgggccgatc aacctggcct ggtcctcctc tggaggcacc ttggatgcgg
      901 atgcggcctg gagcgcggag agcggctcaa ggaggagcac caggcctatg tcgactcagg
      961 cctgaatgtg gtgatagtcg ctcctgccga tttgcaacgt gtgaccctct acaaagagcg
     1021 ataccagatc ccggtcacca tgtactcgga tccggggtac acagcccaca aggccttcgg
     1081 tctcggccac tgggctctcg agcaggccct ctacgacgct ccggacgaat actgcgaact
     1141 gtcggaggag accgggcgtc aattccagga ggatcggcgg tcacaaggac ggcgattggt
     1201 cgacgatcca tggatgcagt ccggtgagtt cgtggtcgca cctgacggga ggatccgggt
     1261 cgcctacctc tacaactact gcgccgacta cccggaccca cgggtgttca ccactgccgg
     1321 ccgtcttctc gggtagggca ccgttcaatg aagacgcttc tgctgatgcg tcacgccaag
     1381 tcggattggg atgccgacta cggctccgat cacgaccggc cactcaacga ccgtgggcag
     1441 aggagtgccc ggctcatggg ccgggtgctg gcggacgagg accttgtccc cgatctgatc
     1501 atcagctcga cagcggtacg cgcgaggcgg acggctgagc tggccatcga agcgggcgct
     1561 tgggacgccg agctcgtcct cgatcgatcg ctatacgacg aggggccgcg aggagtgctc
     1621 gaatcagggg catcggcgcc tgatgtgaac cgggtcatgc ttgtcggcca tcaaccgacg
     1681 tggtcgatgc tggtgtcggc gttgacgggg gagcgtgccg acctgaagac tgcaacggtg
     1741 gctgtcatcg agctcgatct cgattcatgg gcgggactgc ccggggcctc tggcaacttg
     1801 acccgcgtgc tgggaccaaa gacctacgac ctagaaccct gatgaccttt caggtggcgt
     1861 cggtctcggc aatccgggct ttcaccaata tctcgaccag ggtcgcgggc agtggggcgt
     1921 ctgcgtggaa tctcaccgtg cctttggcgg tgtcgtagcc ggcgagctcg gcccggtgtg
     1981 tctcgatcac tgccgggctc tgcacataga aggcacagtg gttcttcccg gcgccgtagg
     2041 agacgagtgg ccggttcttg taatagaagg cgggcacccc gtagccgatc cgttcctccg
     2101 cgtccggggc ggcgcgccgg atcgtctcac ggagatgttg cagcgcggct cccacgtcat
     2161 cgggcagatt ggtgaggaac tcttcgacgt ctgttgggct cggcatgtcg tttccttggg
     2221 tcgggtctgg ggaacaaagg agaggtgatg gttgttgttt gttggtcgca accgtgggtt
     2281 tccgggtgac ttccccgatg accacggtac aatgtcagcc ttccctgatt gcgcggtgcg
     2341 ccttggcgca gcgtgcaaga ggggggttca caaaccatcc cgcatgtgtc atagttgggc
     2401 gtccgggaga tccctgcccg acgggctcag gaggatttga actaaatgcc agccgctgaa
     2461 acgcgtactg agaaaaccga aaagccacgc atccgtcttc attcgaagct gacggacgag
     2521 cgcgggcgtc tcaccacaga cctggtttcc cagtacctga cggccatagg tgagtacgac
     2581 ctcctaactg ccgagcaaga ggtcgagctg gcgcagaaga tcgaggcggg ggaagaagcc
     2641 gccgagaaac tggagaaggg cgagtacaac ggcaagcgcg aggagatgga acttcgccgg
     2701 tttgcccgac gtggtgccca ggccaaagac gatttcctca cggccaatct ccggctcgtc
     2761 gtggccaacg ctcggcgcta tgccaacacc tcggggatcg actttctcga tctgatccag
     2821 gagggcaatc tgggtctcat tcgcgccgtc gagaagttcg actggcgcaa aggcttcaag
     2881 ttctcgacct acgcgacctg gtggatccgt caggcgataa ccagggccat cgccgacaag
     2941 tcacgcacgg ttcgcatccc ggtgcacctc cacgacacgc tggccgcggt ccgcgccgcc
     3001 caggcctccc tgaaggccga gctagggcgg gatccccgac ccgaggagat cgccgaggaa
     3061 gccggcgtca ccgccgacaa agtggagctc gcgctg
//
LOCUS       DASTXO010000225         2270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1413749, whole genome shotgun sequence.
ACCESSION   DASTXO010000225 DASTXO010000000
VERSION     DASTXO010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413749"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="VFU96_04720"
     CDS             <1..988
                     /locus_tag="VFU96_04720"
                     /inference="COORDINATES: protein motif:HMM:NF015368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112598.1"
                     /translation="RRLMAQVWYPALDQGEVDPVLWSEDWDIVAPGVSTLMGFPGWFL
                     SHTKYSWSHGGSSIPVAPGTFPVVIYSHGWTGFRTNSVHQIESLVSNGFIVVAIDHTY
                     GAVATRFPDDEVVEYDPAAIPDPAEVGEELYAEAVEELVNVFSQDIVTVANSLEAGPN
                     GPFGALAESVDLTRLGVYGNETGGGAAVQFCLEDERCDAVLGLDPWVEPIPDRTLAIS
                     AIQPALYMRSDDWIRTENDAILRGIAERSTSTSYWIGVEGTQETDFVGMPLLSPLGAR
                     FGWKGPIPAGRIIPIIDRYLVGFFDVYLLGTGSAALDTSAFEEVSLEVILPD"
     gene            complement(985..1578)
                     /locus_tag="VFU96_04725"
     CDS             complement(985..1578)
                     /locus_tag="VFU96_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU5112599.1"
                     /translation="MTGRVLRWTGLAVVAVTVVFAVVLASRFGSDPGLVDSPLIGQPA
                     PEFTLAGLDGSEGASLSDLRGDIVVVNFFASWCLQCRDEHDDLVAASESFSDQDVRFV
                     QVAYQEEAAESLDYLGEAGASETTLYLADPGSRTAIAYGVFGIPETFFIDPNGTVVGK
                     IIGEADALVLGATIDSIRSGEQPGQQITGDTQQRPDS"
     gene            complement(1575..>2270)
                     /locus_tag="VFU96_04730"
     CDS             complement(1575..>2270)
                     /locus_tag="VFU96_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112600.1"
                     /translation="LWLAPLAVLVLGIAVILWWRRHPGSGDADEPPTPGRSRTRLVVG
                     GVVLVLAFAGIVVVAANSLQDRPAAAAGAAAVDEHDLAEVSNETMEAVIAANTDDPQI
                     NGMRLALAERYFDAGDYRSAFPHYLVVAEDPNASSTEAITALVRLGWMAYAGNGEVET
                     ATRLIDEALAIDPESSVARYVRARVVWCGTGATEQAIETFDDLLADPALAAETRSQIE
                     ADLDLARSGGECP"
BASE COUNT          407 a          750 c          732 g          381 t
ORIGIN      
        1 gagacgtttg atggcgcagg tgtggtatcc ggcgctcgac caaggagagg tggaccccgt
       61 gctctggagt gaggactggg acatcgtcgc ccccggcgtc tccacgctga tgggcttccc
      121 cgggtggttc ctgagtcaca ccaagtacag ctggtctcac ggcggaagct cgataccggt
      181 tgcccccggc acttttccgg ttgtcatcta cagccacgga tggaccgggt tcagaaccaa
      241 ctccgtccat cagatcgaga gtctggtgag caatggtttc atcgtggtgg ccatcgacca
      301 cacctacggg gcggtcgcca cccgattccc cgacgacgaa gtcgtcgagt acgacccggc
      361 agcgatcccc gatccggcag aggtaggcga ggagctctac gccgaggccg tcgaggagct
      421 ggtgaacgtg ttctcgcagg acatcgtgac cgtcgccaat tcgctggagg cggggcccaa
      481 cggacccttc ggcgccttgg ccgaaagcgt cgacctgacc cggctcggcg tctacgggaa
      541 tgagacagga ggtggcgccg ccgtccaatt ctgtttggag gacgagcgat gcgacgcggt
      601 gctcggcctc gatccgtggg tggagccgat tccggatcgg accctggcca tctcagcgat
      661 ccagcccgcc ctgtacatga ggtcggacga ctggattcgc accgagaacg acgccatctt
      721 gcgaggcatc gcagagcgat cgacatccac gagctactgg atcggcgtgg aggggaccca
      781 agagaccgac ttcgtcggca tgccgctgct ctcaccactg ggggcgagat ttggctggaa
      841 ggggccgatc ccggccggac gcatcatccc gatcatcgac cgatatctgg tcgggttctt
      901 cgacgtctac cttctcggca ccgggtcagc cgccctcgac acctcggcat tcgaggaggt
      961 cagcctggag gtcatcctcc ccgactagga atcaggccgt tgttgggtgt caccggtgat
     1021 ctgctgaccc ggctgctcgc cgctgcggat cgagtcgatc gtcgccccga ggacgagggc
     1081 gtccgcctcg ccgattatct ttcccaccac ggtcccattc gggtcgatga agaaggtctc
     1141 cggaatgccg aagacgccat aggcgatggc ggttcggctc cccggatcgg ccagatagag
     1201 cgtggtctcg gaggcgccgg cttcgccgag gtagtcgagc gattctgccg cttcctcctg
     1261 gtacgccacc tgcacgaacc tgacgtcctg atcggaaaat gattccgacg ctgcgaccag
     1321 gtcatcgtgc tcgtctcggc actggagaca ccaggaagcg aagaaattga ccaccacgat
     1381 gtccccgcga agatcggaga gcgaggcgcc ctccgaaccg tcgaggccgg ccaacgtgaa
     1441 ttcgggtgcc ggctgtccga tcaatgggga gtcgacaagg cccggatcgc tcccgaagcg
     1501 gctggccaga accacggcga agaccaccgt gacagccacg acggcgaggc ccgtccagcg
     1561 caagaccctc cccgtcatgg gcattcaccc ccgctacggg ccaaatcgag atccgcctcg
     1621 atctgggacc gcgtctcggc agccagagcc ggatcggcga ggagatcgtc gaatgtctcg
     1681 atcgcctgct cggtcgcgcc ggtcccgcac cagaccaccc tcgctcgcac gtaccgggca
     1741 accgacgatt cggggtcgat ggccagggcc tcgtcgatga gccgggttgc ggtctccact
     1801 tcaccattgc cggcgtatgc catccagccg agacggacca acgccgtgat cgcctcggtg
     1861 ctactggcgt tcgggtcttc ggccaccacg agatagtggg gaaacgccga gcggtaatcg
     1921 cccgcatcga agtagcgctc agcgagagca agtcgcattc cgttgatctg cgggtcgtcg
     1981 gtgttggcgg cgatgaccgc ctccattgtc tcgttgctca cctcggccag gtcgtgctcg
     2041 tcgacggctg cggccccggc ggctgcagcc gggcgatcct gaagtgaatt ggcggcgacc
     2101 accacgattc cagcgaaggc caggaccaga accaccccac cgacgaccag ccgggtccgg
     2161 cttcgcccgg gagtaggcgg ctcgtcggcg tcccccgacc ccggatggcg ccgccaccac
     2221 aggatcacgg ctatccccag tacgaggacg gccaacggcg cgagccagag
//
LOCUS       DASTXO010000226         2323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_50722, whole genome shotgun sequence.
ACCESSION   DASTXO010000226 DASTXO010000000
VERSION     DASTXO010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50722"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1498)
                     /locus_tag="VFU96_04735"
     CDS             complement(<1..1498)
                     /locus_tag="VFU96_04735"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5112601.1"
                     /translation="MQLLERDDILQRLEKLLAAARDGNGRAVLVRGEAGIGKTSAVRA
                     FTDSHLEDTHVLWGGCDDLVTARPLGPIWDMALDEPSLGEVLRGQDRYEVFNLILELM
                     SRSLRPSVIVIEDIHWADEATLDLIKFLGRRIDRSHGLLVLTYRDGQVPGDRPLRVAL
                     ADIAASVLERITLSPLSPAAVSELASEAGGVTDGLWELTGGNPFFLTEVLGAEHETVP
                     GSVRDAVMGRVSRLTPASRALVDLVSVVPGRAELELVDSVLGPSEEAASEAEAAGVIV
                     LKENALTFRHELARRSVETDLPQIRRREINLEVLRAVEDLGYDVSRAAHHARVGGDVD
                     ALVRLAPLAARRAAAMESHSEAVAHLRALEPYLDQLEPEMCADHYDLWAFEEYLGSEI
                     AWAEEIIEIGIGIRRRLDDPEKLGNSLLIGSRIAWVRNRRALATEMANEAASVLESVG
                     GLPLATAYSAISQLAMLAGDEKRTRWYGEKALAVAGEGPSQARAHALNN"
     gene            1565..>2323
                     /locus_tag="VFU96_04740"
     CDS             1565..>2323
                     /locus_tag="VFU96_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5112602.1"
                     /translation="MLALNDLHKHYDDVVALAGCSLQVEKGRMLGFLGPNGAGKTTAM
                     RTIFGLVTPDSGEVTWNGRPISIEQKRRFGYMPEQRGLYPKMKAQEQLTYLGRLRGMD
                     AKEASASADRWLERLGLAERRMDRVEVLSHGNQQRVQLAAALVHDPELLVMDEPFSGL
                     DPIATETMADILREEAERGKAVVFSSHQLDLVEDICEEVAIINAGQIVVEGDVRDLKD
                     AAPIRHLEVELDGEIGDLFDTLEGVRQIRSDDHIH"
BASE COUNT          435 a          759 c          742 g          387 t
ORIGIN      
        1 tgttgttgag ggcgtgggca cgagcctgac ttggcccctc gcccgccact gccagggcct
       61 tttccccgta ccagcgggtg cgcttctcgt cgccggccaa catcgccaat tgcgagatcg
      121 ccgagtaggc ggtcgccaga gggagcccac cgaccgactc gaggaccgat gccgcctcgt
      181 tggccatctc ggtggcaagc gcccgacgat tgcgcaccca ggcgattcgg ctaccgatca
      241 atagcgagtt acccagcttc tccggatcgt ccagtcttcg ccggatgcct atcccgatct
      301 cgatgatctc ctcggcccat gcgatctcac tgccgaggta ttcctcgaaa gcccaaaggt
      361 cgtagtgatc ggcgcacatc tctggttcga gctggtcgag gtatggctcg agggcgcgca
      421 ggtgggcgac ggcttcgctg tggctctcca tggccgcggc gcgtcgcgcc gccagggggg
      481 ccagacgcac cagggcatcg acatcgccac cgacccgggc gtggtgagcg gcccgtgaca
      541 cgtcgtagcc gaggtcctcg acggcccgaa gcacctcgag attgatctcc cgtctcctta
      601 tctgaggaag atcagtctcg accgagcggc gggcgagctc gtgacgaaag gtgagtgcgt
      661 tctccttgag cacgatcacc cccgccgcct ccgcttcact ggcggcctcc tccgagggac
      721 cgagcacgga atccaccagc tccagctcgg cccggcccgg caccaccgaa accaaatcga
      781 ccagtgcccg tgatgccgga gtgagtcgag agactctccc catcacggcg tcacgcaccg
      841 agcccggaac cgtctcgtgc tcggcgccga ggacctcggt caagaagaag gggttgcccc
      901 cggtcaactc ccacaacccg tcggtgacgc ctcctgcctc agatgccaat tcggagaccg
      961 ctgccggaga gagtggggaa agcgtgatcc gctccagcac cgaggcagcg atgtcggcga
     1021 gagcgactcg aagagggcgg tcgcccggca cctgcccatc ccgataggtg agaaccagca
     1081 ggccgtggct ccggtcgatg cgtctgccga ggaacttgat caggtcgagc gtggcctcgt
     1141 cggcccagtg gatgtcctcg atgacgatca cgctgggtcg cagcgaccgg gacatgagct
     1201 cgaggatgag gttgaacacc tcgtagcggt cttgacctcg caagacttcg ccgagtgagg
     1261 gctcgtccag agccatgtcc cagatgggac ccaacggtcg agcagtgacg aggtcgtcgc
     1321 agccacccca caggacgtgg gtgtcctcca aatgggagtc ggtgaaggcg cgaacagccg
     1381 acgtcttgcc gatgccggct tcgccccgca ccaagaccgc ccgcccgttg ccgtctctgg
     1441 cagcggcgag gagcttctcc aaacgctgga gaatgtcgtc gcgctccaac agttgcataa
     1501 gtcgaaggat ggtaacgggg gacccgtctg gctcctatgg gggaccaccc ctatcctccc
     1561 gtcggtgctc gccctgaacg acctgcacaa gcactacgac gacgtcgtcg ccctcgccgg
     1621 atgctcgctt caggtcgaaa agggccgaat gctcggattt ctcggcccca acggagcggg
     1681 caagaccacc gccatgcgca ccatcttcgg tctcgtcact cccgactcgg gtgaggtgac
     1741 ctggaacggg cgaccgatat cgatcgagca aaagcggcgt tttggctaca tgcccgagca
     1801 gcggggcctt tacccgaaaa tgaaggcgca agagcaactg acctatctcg gacggctccg
     1861 agggatggac gccaaggagg cttcggcatc ggccgatcgc tggctggagc gtctcgggct
     1921 ggccgagcgt aggatggacc gggtcgaggt gctgtcgcac ggcaaccaac agcgagtcca
     1981 gctggcagcg gcgttggtgc acgatcccga gctcttggtg atggacgagc cgttcagtgg
     2041 cctcgatccg atcgccaccg agaccatggc cgacatcctc cgcgaggagg cggagagagg
     2101 caaggccgtg gtcttctcct ctcatcaact cgacctcgtc gaagacatct gcgaggaggt
     2161 cgcgatcatc aacgccggac agatcgtggt cgagggagac gtgcgcgacc tgaaagatgc
     2221 ggctccgatc cgtcatctcg aagtcgaatt ggacggtgag atcggagacc tgttcgacac
     2281 gctcgaaggc gtgcgtcaaa tccggtcgga cgaccatatc cac
//
LOCUS       DASTXO010000227         2154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_603414, whole genome shotgun sequence.
ACCESSION   DASTXO010000227 DASTXO010000000
VERSION     DASTXO010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603414"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="VFU96_04745"
     CDS             complement(<1..983)
                     /locus_tag="VFU96_04745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU5112603.1"
                     /translation="MSYNPTTKTSPRPAPTKTRRRYSSGQRWTIRILIFLVLAAATLV
                     ALTFLADFQMEDAGPMPSLAAGDGVYLIVGSDSRENLPDDLEGSFGDFAGARADVIIL
                     AQVVDGRRQLLSIPRDLRVEIPGEGVNKINAAYAFGGPDLLVETVANATGVRPNHYLE
                     VEFGGFAGIVDALGGIELDIPYPARDAKSGLNLDAGVQTVDGATALAYARSRSYEELR
                     DGEWVAQGGGDVARAARQREVLTKIMAEASSPSGLARSPLVVTAVGSHLTADSGLTLF
                     DLAGTGWAMRSAGVTESMTLPVVGAEKGGVSYLVRSEPAATEVLDAFVAGQP"
     gene            1097..>2154
                     /locus_tag="VFU96_04750"
     CDS             1097..>2154
                     /locus_tag="VFU96_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEU5112604.1"
                     /translation="MYAAATSVALVGGDARPVRVEAHVGRQSEAFKLSGLPDTAVREA
                     KDRVRAAVASSGIRFPNRTVTVNLAPADLPKGGTDYDLPIALGILAASREIPPLHDAV
                     VVGELALDGAIRPGSNAFGAGVLSARQNVPCLVASSVAGEAAAIPGATAYGVASLAAA
                     VDLLRGGLRRVEPAQPDACMLGENVPDLADIRGQPGARRALEVAAAGGHHLLMHGPPG
                     SGKTMLARRIVGILPPLQVDEAVETAMIWAAAGRRRGLVATPPFRAPHHTASKAALVG
                     GGSGNPVPGEVSLSHRGTLFLDELAEFPRSHLDTLRQPLEEGVVSVARRGSSIDFPAT
                     FQLLAATNPCPCGFYGDG"
BASE COUNT          342 a          707 c          733 g          372 t
ORIGIN      
        1 ggctgaccgg cgacgaaggc gtcgagcact tcggtcgcgg ccggctcgga tcgcaccagg
       61 taggagacgc cacccttctc cgcgccgacg acggggaggg tcatcgactc ggtgacaccg
      121 gccgagcgca tcgcccatcc agtgccggcg aggtcgaaga gggtcagacc ggagtcggcg
      181 gtcagatgtg aaccgaccgc cgtgaccacc aacgggctac gggcgaggcc cgacggcgat
      241 gacgcctcgg ccatgatctt ggtcagcacc tcgcgctgcc gtgcggcacg ggctacgtcg
      301 ccacctccct gagccaccca ttccccatcg cgcagctcct cgtagctgcg tgagcgggca
      361 taggcgaggg cggtggcgcc atcgacggtt tgcacgccgg cgtcgaggtt gagccccgac
      421 tttgcatccc tggccggata ggggatgtcg agctcgatgc cacccaacgc atccacgatc
      481 ccggcaaacc cgccgaactc cacctcgaga tagtggttgg gccggactcc ggtggcattg
      541 gccaccgtct cgacgaggag gtcgggacca ccgaaggcat aggcggcgtt gatcttgttc
      601 actccctcgc ccggaatctc gaccctcagg tctcggggga tcgacaacag ctgtctccgc
      661 ccgtcgacta cttgggcgag gatgatgaca tcggcccggg caccggcgaa gtcaccaaag
      721 ctgccctcca ggtcgtcggg gaggttctcc cggctgtcac tgcccacgat gagatagacg
      781 ccgtctcccg cggcgagcga aggcatcggt ccggcgtcct ccatctggaa gtcggccagg
      841 aaggtaaggg cgaccaaggt tgcggccgcg agaaccagga agatgagaat gcggatcgtc
      901 cagcgctggc ccgagctgta acgacgacgc gttttggttg gcgcagggcg aggcgacgtc
      961 ttggtggtgg ggttgtagct catggcgatt ggcacgaagg tgccggtatg acggtaaacc
     1021 tgacctaacg cttagtcgcg cacctccggg ctcagccctc ttataacgag ctttgacatt
     1081 ccgtacggtc ggggccatgt acgcagcggc cacgtcggtg gcgctcgtcg ggggagacgc
     1141 ccggccggtc cgggtcgagg ctcatgtcgg acgtcagagt gaggccttca agctctctgg
     1201 cctgcccgat actgcggttc gcgaggcgaa ggatcgggtg cgcgccgccg tcgcctcctc
     1261 cggcattcga tttcccaacc gcacggtcac ggtgaacctg gctccggcgg acctgcccaa
     1321 gggtgggacc gactacgacc ttccgatcgc gttgggcatc ctcgcggcga gcagggagat
     1381 ccctccgctt cacgacgccg tggtagttgg ggagttggct ctggacggcg cgatccgacc
     1441 cggatccaat gcgttcggcg cgggtgtgct gagcgcccgg cagaatgtgc cctgtctcgt
     1501 tgcaagttct gttgccggtg aggcggctgc aatcccgggg gccacggcct atggcgtagc
     1561 cagccttgcg gctgcggtgg acctcctccg tggcggtctg agaagggtcg agcccgccca
     1621 gcccgatgcc tgcatgctcg gggagaatgt gcccgacctg gccgacatca ggggccagcc
     1681 aggggcgaga cgggccctcg aagtggccgc ggccggcggt caccatctgc tcatgcacgg
     1741 gccgcccggc tcaggcaaga ccatgttggc cagacgcatc gtcggcatcc tccctccact
     1801 ccaggtcgac gaggcggtcg agacggcgat gatctgggcg gcggcgggtc ggcgccgggg
     1861 cttggtggcg actccccctt ttcgtgcccc acatcacact gcttccaaag ccgccctggt
     1921 cggcggtggt agcggcaatc cggtccccgg cgaggtttca ctttctcatc gagggacgtt
     1981 gtttctcgat gagctggcgg agtttccccg cagccatctc gacacgcttc gccaaccctt
     2041 ggaggaaggc gtggtatccg tcgcccgccg agggtcatcg atcgatttcc cggcgacctt
     2101 tcagctcctg gccgcgacca acccatgccc gtgcggcttc tatggcgatg gcag
//
LOCUS       DASTXO010000228         3830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1303498, whole genome shotgun sequence.
ACCESSION   DASTXO010000228 DASTXO010000000
VERSION     DASTXO010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303498"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..145
                     /gene="eno"
                     /locus_tag="VFU96_04755"
     CDS             <1..145
                     /gene="eno"
                     /locus_tag="VFU96_04755"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005962116.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU5112605.1"
                     /translation="ATNSGQIKTGAPARGERTAKYNQLLRIEESLGGEARFAGWDSFR
                     RAG"
     gene            142..540
                     /locus_tag="VFU96_04760"
     CDS             142..540
                     /locus_tag="VFU96_04760"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU5112606.1"
                     /translation="MTLIRRPGVALVTLLFLLLGAAFITQVVPYRQIIDSQRQVTAAQ
                     ERLATLEEENAALQADAAALNTDEEIEKLAREKLGYVRPGETAYVVLDPPGASQSAAD
                     AEEEGLTVDERTWVDRIWDFLSGGDLDSGG"
     gene            537..1055
                     /locus_tag="VFU96_04765"
     CDS             537..1055
                     /locus_tag="VFU96_04765"
                     /inference="COORDINATES: protein motif:HMM:NF016314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HEU5112607.1"
                     /translation="MTPSDDRQVVEIQIGRPLRAESSPVSRCHLGLPVVVRVPPILDD
                     GTPFPTLYWLTCPLAVGRIARLEGAGGIKRLERKAEVDANFGTRLTEAHEAYEAERQT
                     LLPPDASNLPSGGVGGTRTGIKCLHAHYAHARAGGDNPVGELVQDWIEPLDCTVPCVL
                     DGEMNPDWIARP"
     gene            1037..1927
                     /locus_tag="VFU96_04770"
     CDS             1037..1927
                     /locus_tag="VFU96_04770"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEU5112608.1"
                     /translation="MDRPPVRVGVVDIGTNSMRLLVTDGSVDIDREVEVTGLGRGVDR
                     TGVLSPEGIEESLAALARFRTGMDRAEVEKRAAIATSASRDAANREDFFDLAESALGV
                     RPSLITGEEEARLAFAGATLGIDAIDPIVVSDIGGGSTEFVTVGGEESVDIGSVRLTE
                     RVIGTRPASSQEMSAARELVAGLFAAVALETGTLIGVAGTWTSLAAIAQELPEYSPQR
                     VHGHRMGRGALTDVIAALSTMTVEETAAIPALDPRRAPVILGGALVAEGVMDTLDIGE
                     VTISERDTLDAVAMELLAIP"
     gene            1940..2024
                     /locus_tag="VFU96_04775"
     tRNA            1940..2024
                     /locus_tag="VFU96_04775"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1972..1974,aa:Leu,seq:taa)
     gene            2074..2445
                     /locus_tag="VFU96_04780"
     CDS             2074..2445
                     /locus_tag="VFU96_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112609.1"
                     /translation="MTEKKVAAPADKVELYMQLLSSVPEIDAKSNFGSAYTAINGNMY
                     TMISKHGVVGIRLPEPERAEFLQTHSSELFRGDPSWPPAKEFVAVPDRLLERTEVLTP
                     YLESSLRYVKGMKPKSTKTKS"
     gene            2451..2813
                     /locus_tag="VFU96_04785"
     CDS             2451..2813
                     /locus_tag="VFU96_04785"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEU5112610.1"
                     /translation="MRNPEVDAWFEAKQHPMEEAMQTVRRVTLEADPRITESIKWSTP
                     TYSYQGNIFSFNPAKKVVSLLFHTGAQIPGDHPALEGDTATARVMRFSDAADVERRAG
                     ELVEVLRSWCEWKDASSP"
     gene            3066..>3830
                     /locus_tag="VFU96_04790"
     CDS             3066..>3830
                     /locus_tag="VFU96_04790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5112611.1"
                     /translation="MAEYPSEYELDVALRDGGGARIRPIKPDDGPLLVEFFERLGRES
                     RYFRFFRIKETLEPKEVEFFTNVDYSDRMALIAVLDGRMIGLASYDREPDKDGTAEVA
                     FAVADEHQGRGIGTQLLQLLTNHARSQGVRHFEAFVLPENRQMMRLFRNSGYELTRTI
                     DEGVFTVDFPVAESEGMLQAEWEREKRAVAASILPLFFPRSVAVIGASTDPTSIGGRL
                     FRNILNGGFNGPLYPVNPKAKVIGSVRAYATISDVPD"
BASE COUNT          680 a         1198 c         1303 g          649 t
ORIGIN      
        1 cgcgacgaac tccggccaga tcaagaccgg tgcccccgcc cggggcgagc gaacggccaa
       61 gtacaaccag ctgctccgca tcgaggagtc cttggggggc gaggcccgtt tcgcgggatg
      121 ggacagcttc cggagggcgg gttgacgctc atacgccgcc ccggcgtcgc cctcgtcaca
      181 ctcctcttcc tgcttcttgg cgctgcgttc atcacacagg tcgtgcccta ccgacagatc
      241 atcgattcac agcgccaggt caccgcagcc caggagcgcc tggccactct cgaggaggag
      301 aacgcggcac ttcaggccga tgccgcggcc ctgaatacgg acgaggagat cgagaaactg
      361 gctcgggaga agctcggcta cgttcgcccc ggtgagacgg catacgtggt gctcgaccca
      421 cccggggcca gccaatcagc cgccgacgcg gaagaagagg ggttgacggt cgacgagagg
      481 acctgggtgg atcggatctg ggatttcctc agcggcggcg acctagattc ggggggatga
      541 ccccctccga tgaccgtcag gtcgtcgaga tccagatcgg gcgcccattg cgggcggaat
      601 cgagcccggt ctcgcgatgt catctcggac tgcccgtcgt cgtcagggtc ccacccatcc
      661 tcgacgatgg gacaccgttc cccacgctct actggctgac ctgcccgctc gcagttggcc
      721 gtatcgcccg gctcgaggga gccggcggga tcaagcgcct cgaacgcaag gccgaggtcg
      781 acgcgaattt cgggaccagg ctgaccgaag cgcacgaggc ctacgaggca gagcggcaga
      841 ccttgctccc tccggacgcc tcgaatctcc cctcgggagg cgttggtggc acccggaccg
      901 gcatcaagtg tctccacgcc cactatgcgc acgcgagggc aggtggtgac aatccggtgg
      961 gagagttggt ccaggactgg atcgagccgc tggattgcac cgtgccctgt gtgctggatg
     1021 gcgagatgaa tccggattgg atcgcccgcc cgtgagggtg ggagtggtcg acatcggaac
     1081 caactcgatg cggctgctgg tgacagacgg gtcggtggat atcgatcggg aggtggaggt
     1141 aacggggctg ggccgcgggg tcgatcgaac cggcgttctc tccccggagg ggatcgagga
     1201 gtcgctggcc gccctcgccc gttttcggac ggggatggac cgggcggagg tggagaagcg
     1261 ggcggcgatc gcaacgtccg cctcccgcga cgcggcgaac cgagaggatt tcttcgacct
     1321 cgccgagtcc gccctcggcg tgcgcccgag tctgatcacc ggggaggagg aagctcgcct
     1381 tgcctttgcc ggcgccactc tcgggatcga cgccatagac ccgatcgtgg tctcggacat
     1441 cggtggtggc agcacggagt tcgtgacggt cggaggagag gagtcggtcg atatcgggtc
     1501 ggttcgcctc accgagcgag tgatcggtac tcgccccgcg tcgtcgcagg agatgagcgc
     1561 agcccgtgag ctggtggctg ggctgttcgc cgcggtcgcc ctggagaccg gcacgctgat
     1621 cggggtcgcc gggacatgga cgagtctggc ggcgatagca caggagcttc ctgagtattc
     1681 gccccagcgt gtgcacggtc accggatggg ccgtggcgcc ctgacggacg tgatcgcagc
     1741 cctgtcgacg atgactgtcg aggagacggc cgccattcct gccttagatc cccgccgggc
     1801 tccggtcatt ctcggggggg cgctcgtcgc cgagggggtg atggacaccc tcgacatcgg
     1861 cgaggtgacg atctcggagc gcgacactct cgacgccgtc gcaatggagt tgctggctat
     1921 accctgatgc ctggcccgcg cccgggtggc ggaatgcaga cgcgaggggc ttaaaccccc
     1981 ttgagccgaa tcggctcgtg tgggttcgaa tcccaccccg ggcacgagtg tggattgccc
     2041 ctctctacga tcggtctacc acagaggtgc gcgatgactg agaagaaggt ggctgccccc
     2101 gctgacaagg tggagcttta catgcagctc ctgagcagcg tccccgaaat cgacgccaag
     2161 tcgaatttcg ggtcggccta cacggccatc aacgggaaca tgtacaccat gatctcaaag
     2221 cacggggtcg ttgggatccg cctacccgag ccggagcggg ccgagttcct gcagacacac
     2281 tcgagcgagt tgttccgcgg cgatccgtcc tggccaccgg cgaaggagtt cgtggcggtg
     2341 cccgaccgcc tcctcgagag aaccgaggtc ctcacgcctt atctcgagtc gagtcttcgt
     2401 tacgtcaagg gaatgaagcc gaagtcgacg aagaccaaga gctagtcacg gtgcgaaatc
     2461 ccgaagtaga tgcgtggttc gaggcgaagc agcatccgat ggaggaggcg atgcagaccg
     2521 tgcggagggt gacgctggag gctgatcccc gcatcacgga gtcgatcaaa tggtcgacac
     2581 cgacctactc ctatcagggg aacatcttca gcttcaaccc ggcgaagaag gtcgtcagcc
     2641 tcctatttca caccggggcg cagatccctg gggaccaccc ggctttggag ggtgacaccg
     2701 cgaccgcccg cgtcatgcga ttctccgatg cggcggatgt ggagcgccgc gccggagagc
     2761 tggtcgaagt cctccgttcg tggtgcgagt ggaaggacgc gtcttcgccc tgaagttctc
     2821 gcccgagccc agtgtgggcc gttcgcattg tcgaggatcc cgatcgcagg cttgcgctgg
     2881 agaggtcgtg ggacccgggc cggtgggtgg gggaggggag aggaaccggg cccgggcccc
     2941 acggtgcgcg atggaagatc tacgtccctg tgtttctcct ttcccacata gatcaacccc
     3001 gtgaccacaa gtctaacccc cgaccggccg cgctggcctc aggtaagtcg cggtagcgtt
     3061 gggtgatggc cgagtatccg tccgagtacg agctggatgt cgctcttcga gacgggggcg
     3121 gggcgcggat ccggccgatc aagcccgatg acggccctct cctcgtcgaa ttcttcgaaa
     3181 ggctcggccg cgaatcccga tacttccgct tcttcaggat caaggagacg ctggagccga
     3241 aggaagtcga attcttcacg aatgtcgatt actcggaccg catggcgctc atcgccgtgc
     3301 tcgacggcag gatgatcggg ctggccagct acgaccgtga gcccgacaag gatggcacgg
     3361 cggaggtggc gttcgccgtc gccgatgagc atcaagggcg cggaatcggg acacaactcc
     3421 tccaactcct caccaaccac gcccgatcgc agggcgtgcg gcacttcgag gcattcgtgc
     3481 tgccggagaa tcggcagatg atgaggctgt tccgaaactc gggatacgaa ctgacccgca
     3541 ccatcgacga gggcgtcttc acagtcgact tccccgtggc ggagagcgag ggcatgcttc
     3601 aagcggagtg ggagcgtgag aagcgcgccg tcgccgcctc gatcctccca ttgttcttcc
     3661 cccgatcggt cgcggtcatc ggggcctcga ccgacccgac gtccatcggc ggtcgcctgt
     3721 tccgaaacat cctgaacggt ggcttcaacg ggcccctgta cccggtcaat cccaaggcca
     3781 aggtgatcgg gtcggtccgg gcctacgcga cgatcagcga cgttcccgac
//
LOCUS       DASTXO010000229         8020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1266466, whole genome shotgun sequence.
ACCESSION   DASTXO010000229 DASTXO010000000
VERSION     DASTXO010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8020
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266466"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1264
                     /locus_tag="VFU96_04795"
     CDS             <1..1264
                     /locus_tag="VFU96_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEU5112612.1"
                     /translation="PNGSVIDAASGTWLYGQCSVNRWFVPEPALAQADRILFMDLDGD
                     SGQDLLALGGGSPVQAIVATRDSDFEELSTISTGLDPASVAVAGADYNGDRRADLWEA
                     RPSGGLRILAGPQFTTALRNASLPSGTPLFIASGDRDGGNTPELFALYPSSGGSRIAV
                     IDLEGGVTVEQSFDISVPAAQVRGLGAADYDGDGRADIEVLTRNGRFSVHVGNTPTGA
                     PATRWFARPDQTCDDPVRLAFEGLFYDDEDSIFGSAIDRLGASGITRGCNPPFNDAFC
                     PDDPVTRGQMAAFLVRALGYTAEGGVDQFDDDNTSVFESAIDRLATAGVTRGCNPPAN
                     TRFCPDDPVTRGQMAAFLVRALGYTNDGGGNRFTDDNSSVFESAIDRLATAGVTLGCN
                     PPANTRFCPDQPVTRGQMAAFLTRALRL"
     gene            complement(1268..2383)
                     /locus_tag="VFU96_04800"
     CDS             complement(1268..2383)
                     /locus_tag="VFU96_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEU5112613.1"
                     /translation="MSTSSEPSLTGFDYPGRVTRLQSTMDAEGVDAVVLSIGADLPYF
                     TGYEAMDSERPTVLIIARTGIPVLVIPLLEAPRVEATNIELASWRETEDPMVLVVRAL
                     GAARRVAIGDQTRSSTLLSIQERLPDATWVGASDLTSTLRARKEPAEIEMLRSAANAV
                     DRALARVPAELRFSGRTEMDVARDLQTMTVEEGHDVAMFAIVGAGGNGASPHHEPGNR
                     VIEPGDMVVCDFGGRRNLYTSDVTRTFSVGGPSQEQVEVHRVVLAASEAARAAVGPGV
                     ACQEIDRAARDVIESAGFGEFFIHRTGHGIGLEVHEHPYIVEGNATPLEEGMTFSVEP
                     GIYLRGRFGVRIEDIVTCGPHGVDELNQADRSLRVVD"
     gene            complement(2367..3623)
                     /locus_tag="VFU96_04805"
     CDS             complement(2367..3623)
                     /locus_tag="VFU96_04805"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEU5112614.1"
                     /translation="MLRRVLVLLVAAGLLVPAIPQTSVEAQETVGQYSTSVARLQQEL
                     KDKKLYRGSVDGIYGSQTQQAVMAFRKEIGASRSFSWSDSLWDDLELYQGPYSRFDEP
                     DRVEVNLTKQTAHLFRNGDLVATFPISSGNGERYFANGSWRTANTPTGDYEVYRRARG
                     WYESTLGLGLMLDPWFFNGGIALHGSTSVPSGPASHGCIRLTTWDSGFLNSHMFIGMP
                     VHVYRSNGSPVYGSDGPFADVPADHTFVAAVQWMVDTGVSEGCRLYFFCPDARASRGE
                     IAAFMRRVLLPYLGPAPPVEFADTEGHQFERAIEWMAGHGITKGCNPPANTQFCPDRA
                     VSRGEMAAMFMRAVEELSGVSSDNVNLDRFRDTRGHNFQKAAAWMAAAGISVGCNPPS
                     NDLYCPDTKVTRAQLSAFLKRIVDQL"
     gene            3733..5133
                     /locus_tag="VFU96_04810"
     CDS             3733..5133
                     /locus_tag="VFU96_04810"
                     /inference="COORDINATES: protein motif:HMM:NF045027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEU5112615.1"
                     /translation="MSRRAGPLLVMVAVTVAMSVALPAGAEIVVQNGFEDETWTEGMI
                     DVRSLDLSRTNVIGGGFAGNGLRVVIPEGGFRGLGPLDRLEPAPREAWYRYHIRLLSW
                     NAAFVGKLPGLAGLYSSSGRGCIPPTESSPGWSARGLFGSPGTEGAPAGRIPIGTYLY
                     HADQPGDCGEHLWWPGASLQQGRWHCIEGHVTLNDPWARNGAFQGWLDGKLRFSKQNI
                     AYRSPGEANIRIRHMWHNVYFGGDWPSPNRLSLVFDQVAVSTDGRIGCMDPFKDDNGS
                     IFKPALSELHARGLLYGCGYRLACPDRTITRGEAAAFFHRVLGLPATGRDFFIDDGNS
                     IFEGAINRLAAAGIVRGCEERRFCPDRNLTRAEFAAMTVRALDLPRDNADAFSDDNRH
                     WAESAIDAFAAAGITKGCGSDAFCPDRRLTRGEASAFFVRVDDLNQPIGLASVPSPPE
                     YPPPGDPPPIPPEEQD"
     gene            5162..6316
                     /locus_tag="VFU96_04815"
     CDS             5162..6316
                     /locus_tag="VFU96_04815"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HEU5112616.1"
                     /translation="MRLPVLLVSLALAVLPPGGTFIDDDGNVHEGGIEAISAAGITVG
                     CNPPAGDRYCPDDPTSRGEMAAFLARATDLPDGWQDFFVDDDGHRFETAIDRIAAARI
                     SLGCDPPRNERFCPDEDMTRGQMAMMLARAFDLPSTTKDHFVDDEASVAEQAIDRIAV
                     AGVTLGCNPPANDRYCPDAVVSRAQMATFLTRALGLSPLVPPSRPRPVLGVVSRETWG
                     AAAPALSEMVPHVIHQITVHHAGTSSDHTSTARIRGWQEEHMAGHGWPDLAYHIVIGV
                     DGTLYEGRDPAYRGDTNTSYDTTGHLLVMLDGNFEVQHPPMAQLETLISVLAWAAQRY
                     HVAPATIGGHSDHAATLCPGRHLEHLIHSGELAAEVQQLLDAGGVDLRWP"
     gene            complement(6331..>8020)
                     /gene="lipA"
                     /locus_tag="VFU96_04820"
     CDS             complement(6331..>8020)
                     /gene="lipA"
                     /locus_tag="VFU96_04820"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF004019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEU5112617.1"
                     /translation="HQTKPRDLGLGAGTGVTLRTRWLGRLPYTEAWDLQRAIHEGKVV
                     GRTRDDYLLLLEHPPVFTIGRNGDGSNLLVDRVGLGADVHHVDRGGDITFHGPGQLVG
                     YPILRLDDPKQIVPYVRRLEQMLIETLSDFGVDAWAEPGYTGVWTARGKIAAIGVRVA
                     RRVTMHGFALNLHPDLAYFGMMNPCGITDRPVTTLSELVGRKVTLQEAMERLIPHFER
                     VFEYRVSETQLGAFSRGQGARSEFEVDRLVEAGTFSQEKRTAEPILINGRLPGEPARP
                     DWMKVTARLDGDYLELKKMMRGLQLNTVCEEANCPNIYECWGMGTATLMILGDKCTRA
                     CSFCNVTTGKPTEFDIFEPFRAAEAIHQMNLKHAVITSVNRDDLDDGGAAIFAHTIRE
                     SRRRSPGTEIEVLIPDFKGDREALQTVMDAVPEVLNHNTETVLRLQRDIRTSASYGRS
                     LSLLWRAKQMNPEGLTKSGLIVGMGETREEVLGALADMRAVGIDIITIGQYLRPTARH
                     RPIHRYVHPDEFAEYKSIGEGMGIPHVESGPLVRSSYHAKESRESVPVSIGSRT"
BASE COUNT         1385 a         2685 c         2520 g         1430 t
ORIGIN      
        1 tcccaatgga tccgtgatcg atgccgcctc aggaacctgg ctctatgggc aatgctcggt
       61 caaccggtgg ttcgtgcccg agcccgcgtt ggcccaggcg gaccggatct tgttcatgga
      121 tctggacggc gacagtggac aggacctgct cgctctaggc ggtggatcgc cagtccaagc
      181 catcgtcgct actcgtgatt cggacttcga ggagctgtcg acgatatcga ccgggctgga
      241 tccggccagt gtggctgtgg ccggggcgga ctacaacggc gaccgccggg ccgacctgtg
      301 ggaggcccgt ccctctggtg gcctccgcat cctggccggg ccacagttca cgacagccct
      361 gcgcaacgcc agcctgccct cgggcacacc gcttttcatc gcgtccggag accgggacgg
      421 aggcaacacg ccggagctct tcgccctcta tccctcgagc ggcggttccc gcatcgcggt
      481 catcgatctc gagggcggtg tgaccgtcga gcagagcttc gacatatcgg tgccggcggc
      541 ccaggtacgc ggcctcggag ccgctgacta cgacggcgac ggtcgggcag acatcgaggt
      601 cctcacccgg aatggccgat tcagtgtcca cgtcggcaac acaccgaccg gtgcccccgc
      661 cacccggtgg ttcgcccgtc ccgaccagac ctgcgatgac ccggtccggc tcgccttcga
      721 gggtctcttc tacgacgacg aagacagcat cttcggctcc gcgatcgatc gactgggcgc
      781 ctcggggatc acccgcggtt gcaacccgcc attcaacgac gccttctgcc cggacgaccc
      841 ggtgacgaga gggcagatgg ccgccttcct cgtccgtgcc ctcggctaca ccgccgaggg
      901 gggcgttgac cagttcgacg acgacaacac aagcgtcttc gagtcggcga tcgaccgcct
      961 ggccaccgcc ggagtcactc gcggctgcaa cccaccggcc aacacccggt tctgtcccga
     1021 cgacccggtg accagagggc agatggccgc cttcctcgtg cgagcgctgg gctacaccaa
     1081 cgacggaggc ggcaatcgtt tcaccgacga caattcaagt gtcttcgagt cggcgatcga
     1141 ccgcctggcc actgccgggg tcaccctggg ctgcaaccct cctgccaaca cccggttctg
     1201 tcccgatcag ccggtgactc gtggccagat ggccgccttc ctgacgcgag cactccgact
     1261 ctgaggatca atcgacgacg cgcagcgacc gatcggcctg attcaactcg tcgactccgt
     1321 gagggccaca ggtcacgatg tcctcgatcc tgaccccgaa gcggcctcga agatagatcc
     1381 cgggctcgac ggagaatgtc atgccctctt cgagcggcgt cgcgtttccc tccactatgt
     1441 agggatgctc gtgaacctcg agtccgatcc cgtgaccggt gcgatggatg aagaactcac
     1501 cgaaaccggc ggattcgatc acgtctctcg ccgcgcggtc tatctcctga caggccactc
     1561 caggaccgac cgccgcccgt gccgcctcac ttgcggcgag cacgacgcgg tgcacctcca
     1621 cctgctcctg ggaaggcccg cccacggaga aagtgcgggt cacgtctgag gtgtagaggt
     1681 tgcgtcggcc accgaaatcg cagaccacca tgtccccagg ttcgatgacc ctatttcccg
     1741 gctcgtgatg gggagaggcg ccgttcccgc cggcaccaac gatggcgaac atcgcgacat
     1801 cgtgtccttc ttccacggtc atcgtctgca gatcccgggc gacatccatc tctgttcgac
     1861 cggagaagcg gagctcggcc ggcacccggg cgagggctcg atcgacggcg ttcgccgccg
     1921 atctcagcat ttcgatctcg gccggctcct tgcgcgctcg cagtgtcgac gtgagatccg
     1981 aagcgccgac ccaggtcgca tcgggcagac gctcctggat cgaaaggagc gtcgacgaac
     2041 gtgtctggtc tccgatggcg acgcggcgtg ccgctcccaa ggcgcgaacg accagcacca
     2101 tcgggtcctc ggtctctctc cagctcgcca gctcgatatt ggtcgcctcg actcgtggcg
     2161 cctcgaggag agggatcacc agcacgggaa tgccggtgcg ggcgatgatc aacaccgtcg
     2221 gtcgctcgga atccatggcc tcgtaaccgg tgaagtaggg caggtcggca ccgatggaga
     2281 ggacgacggc gtcaacaccc tccgcatcca tggtcgattg cagtcgggtg acgcgtcccg
     2341 ggtagtcgaa gccggtcaac gacggctcag agctggtcga caatccgctt caggaaggcg
     2401 gagagctggg cacgggtcac cttggtgtcc gggcagtaga ggtcgttgct cggcggattg
     2461 cagccgacgc tgatcccagc ggcggccatc cacgccgcag ccttttggaa gttgtgaccc
     2521 cgcgtgtcgc ggaagcggtc gaggttcacg ttgtccgagc tcacgcccga cagctcttcg
     2581 acggcgcgca tgaacattgc ggccatctct cctcgggata cagcgcggtc gggacagaat
     2641 tgggtattcg ccggcgggtt gcaccccttg gttatcccgt gcccagccat ccattcaatt
     2701 gcccgttcga actgatgccc ctcggtgtct gcgaactcaa cgggcggggc cgggcccaga
     2761 taaggcaaga gaactcgcct catgaaggcg gcgatctcac cacggctggc acgggcatcg
     2821 ggacagaaga agtagagcct gcacccctcg ctgacgccgg tgtcgaccat ccattgcacc
     2881 gcggccacaa aggtgtgatc ggcggggacg tctgcaaacg ggccgtcgct tccgtaaacc
     2941 ggggagccgt tggaccgata cacatgcacc ggcatcccga tgaacatgtg gctgttgaga
     3001 aatccgctgt cccaggtggt cagccgaatg catccgtgtg acgccggccc ggatgggacc
     3061 gaggtcgagc cgtgaagcgc gatgcctccg ttgaagaacc aggggtcgag catcagcccc
     3121 aggccaaggg tcgactcgta ccagccccgc gcccgccggt agacctcgta gtccccggtg
     3181 ggagtgttgg cagtccgcca tgagccgttt gcgaaatacc gctcgccgtt tccggacgag
     3241 atcggaaagg tggccaccag atcgccgttt cggaagagat gcgccgtctg tttggtcagg
     3301 ttgacctcga ctcggtcggg ctcatcgaac ctgctgtacg gcccctggta caactcgaga
     3361 tcgtcccaga gtgagtcact ccatgagaag gaacggctgg ccccaatctc tttccggaat
     3421 gccatgaccg cctgttgcgt ctgcgatccg tagatcccgt cgaccgatcc ccggtagagc
     3481 ttcttgtcct tcaactcctg ctggagtcgg gcgacactcg tcgagtattg cccgactgtc
     3541 tcctgtgcct ccaccgaagt ctggggaatc gccgggacga ggagcccggc ggcgaccaac
     3601 aagacgagga ccctgcgcag catgagggcg ccagcctatc ggatcgtcgg atttgcgccc
     3661 acactctccc cgcgtggtcg ctacccactg agggtgttca gaaatacgag catcggcgct
     3721 acggtgaacc cgatgagccg aagagcgggc cctctgctcg tcatggttgc cgtgaccgtc
     3781 gccatgagtg tggcgctccc ggctggggcg gagatcgtcg tccaaaatgg cttcgaggac
     3841 gagacttgga ccgagggaat gatcgatgtc agatccctcg acctgtcccg caccaacgtg
     3901 atcggtggtg gtttcgcggg caacgggctc agggtggtca tccctgaggg agggttccgt
     3961 ggcctcggcc cgctggacag actggagccc gccccgcgtg aggcctggta tcgctatcac
     4021 attcgcctgt tgagctggaa tgccgccttc gtcggaaagc tccccgggct cgccggcctc
     4081 tattcgtcat cggggagggg ttgcatcccc ccgaccgaga gctcccccgg ttggtccgct
     4141 cgtggcctgt tcggcagccc gggaaccgaa ggtgcaccgg caggccgaat cccgatcggg
     4201 acgtacctct atcacgccga ccagccgggt gactgcggtg agcacctctg gtggccaggg
     4261 gcttccctgc agcagggtcg ctggcattgc atcgaagggc acgtcacgct gaacgatcca
     4321 tgggcccgca atggggcctt ccaaggctgg ctcgatggga agctccgctt ctccaagcag
     4381 aacatcgcat accggtcccc aggcgaagcg aacatccgga ttcgacatat gtggcacaac
     4441 gtctatttcg gtggagattg gccctccccg aaccgcctct cgttggtctt cgatcaggtc
     4501 gccgtgtcta ccgatggccg catcggctgc atggacccgt tcaaggacga caacgggagc
     4561 atcttcaagc ctgccctgtc cgaattacac gctcgggggc tgctctacgg atgcggctat
     4621 cgactggcct gtccggatcg aaccatcacc agaggcgagg ccgcggcctt cttccaccgc
     4681 gtgctcggac tgcctgccac cggccgcgac ttcttcatcg atgacggaaa cagcatcttc
     4741 gagggtgcca tcaaccgcct ggccgcggca ggcatcgtga ggggttgcga agagcgacga
     4801 ttctgccccg accggaacct gacccgcgcc gaattcgccg ccatgacggt gcgggcgctg
     4861 gacctacccc gtgacaatgc ggatgccttc tcggatgaca atcgacactg ggcagaatcg
     4921 gcgatcgatg cattcgccgc cgcagggatc accaagggat gtggatcaga cgctttctgc
     4981 cccgatcgga gactgactcg gggcgaggcc tctgcattct tcgtccgcgt tgacgacctg
     5041 aaccagccaa tcggcctggc ttcggtcccg tcgccgcccg aatacccgcc accgggtgat
     5101 ccaccaccca tccctccgga ggagcaggac tgatcgccgg gcaaggacga gactggtgac
     5161 gatgcgactg cccgtcctac tggtctcctt ggcactggcg gtgctgccgc ccggggggac
     5221 tttcatcgac gacgacggca acgttcacga gggtgggatc gaggccatct ccgccgcggg
     5281 aatcacagtc ggatgcaatc ctccggccgg cgaccggtat tgccccgacg acccgacgag
     5341 ccggggggag atggcggcct tcctcgcccg agcgaccgac ctgcccgatg gctggcagga
     5401 cttcttcgtc gacgacgacg gtcatcgctt cgagacggcg atcgatcgca ttgccgcggc
     5461 acgcatctca cttggctgcg atccaccgcg aaacgagcgg ttctgcccgg acgaggatat
     5521 gacacgaggg cagatggcga tgatgctggc gagagccttc gatctgccct ccacgacgaa
     5581 ggaccatttc gtcgacgacg aggccagcgt cgccgagcag gcgatcgatc gcatcgccgt
     5641 ggccggcgtg accctgggct gcaacccacc ggccaacgac cgctactgcc ccgacgccgt
     5701 ggtgagccgg gcgcaaatgg ccacattcct cacccgcgcc ctcggtctct caccactcgt
     5761 ccccccgtcc cggccgcgcc cggtgctcgg tgtcgtgtct cgagagacat ggggtgcggc
     5821 cgcaccggcc ctctccgaga tggtgcccca tgtcatccat cagatcacgg tgcatcacgc
     5881 cggcacctcc tccgatcaca ccagcacggc ccggatcagg ggatggcagg aggagcacat
     5941 ggccgggcac ggctggccgg atctcgccta tcacatcgtg atcggggtgg acggcactct
     6001 gtatgaaggg cgcgatcccg cctaccgggg cgacaccaac acctcctatg acacgaccgg
     6061 gcacctcctc gtgatgttgg acgggaactt cgaggttcag caccccccca tggctcagtt
     6121 ggagaccctg atttcggtct tggcctgggc cgcacagcgc taccacgttg cgcccgccac
     6181 catcggtggc cacagcgacc acgccgccac cctgtgccct gggcgacatc tcgagcatct
     6241 gattcactca ggggagctgg cagccgaggt gcaacaactg ctcgatgccg gcggcgtcga
     6301 tctccgctgg ccctgagcgg ttccagatct tcaggtgcga gacccgatgc tcaccgggac
     6361 cgattcccgg ctctccttgg cgtgataact ggagcggacg agagggccgg actcgacatg
     6421 ggggatgccc atgccttccc cgatggactt gtactcggcg aactcgtcgg gatggacgta
     6481 gcggtggatg gggcggtggc gggccgtggg tcggagatat tggccgattg tgatgatgtc
     6541 gatgcccacc gcgcgcatgt cggccagggc tcccaacact tcctcgcggg tctcacccat
     6601 ccccacgatc agccccgact tggtgagtcc ttccggattc atctgcttcg ccctccagag
     6661 cagagatagc gagcgtccgt agctggccga ggtccggatg tcgcgttgca gccggagcac
     6721 ggtctcggtg ttgtggttta gcacctcagg cacggcatcc atgaccgttt gcagggcctc
     6781 gcggtcaccc ttgaaatcgg ggatcagcac ctcgatctcg gtgccgggcg aacgccgccg
     6841 gctctcccgg atggtgtggg caaagatcgc ggcgccaccg tcgtcgaggt cgtcgcgatt
     6901 gaccgacgtg atcacggcgt gcttgagatt catctggtgg atcgcctctg ccgcccggaa
     6961 cggctcgaag atgtcgaact cggttggctt gcccgtcgtc acattgcaga acgagcaggc
     7021 gcgagtgcat ttgtccccga ggatcatcaa tgtcgccgtc cccattcccc agcactcgta
     7081 gatgttggga cagttggcct cttcgcacac ggtgttgagc tgaagcccgc gcatcatctt
     7141 cttgagctcc aggtagtcgc cgtcgagccg cgccgtcacc ttcatccaat caggccgggc
     7201 tggctcgcct ggcagccgcc cgtttatgag aatcggctca gcggttcgct tctcctgact
     7261 gaaggtccct gcctccacca accggtccac ctcgaattcc gagcgcgctc cctggccacg
     7321 ggagaaggcg ccgagttgag tctcggacac ccgatactcg aacactcgct cgaagtgcgg
     7381 gatgagcctc tccatcgcct cttggagcgt gacttttctc cccaccaact cggacagagt
     7441 cgtcaccgga cggtcagtga ttccacaggg gttcatcatc ccgaaatagg cgagatctgg
     7501 gtggaggttc aacgcgaacc cgtgcatcgt cacccgacgg gcaaccctga cgccgatggc
     7561 cgcgatcttg cccctcgccg tccacacgcc ggtgtagccg ggctcggccc atgcgtcgac
     7621 gccgaagtcc gacaaggtct cgatcaacat ctgctcgagc cggcggacgt agggaacgat
     7681 ctgcttgggg tcgtcgagac gcaggatcgg gtaacccacc agctgaccgg ggccgtggaa
     7741 ggtgatgtcc ccgccgcgat cgacgtggtg cacgtcggcc cccaggccga ccctgtcgac
     7801 gaggaggttc gagccatccc cattgcggcc gatggtgaac accggcgggt gctcgagcaa
     7861 cagcaggtag tcgtccctgg tccgaccgac gaccttgccc tcgtggatgg cgcgctgcag
     7921 gtcccacgcc tcggtgtaag gcagccggcc gagccagcgg gtgcgaagcg tcacgccagt
     7981 tcctgctccc aatcccaggt ctcgaggttt tgtttgatgc
//
LOCUS       DASTXO010000230         2545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1193190, whole genome shotgun sequence.
ACCESSION   DASTXO010000230 DASTXO010000000
VERSION     DASTXO010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193190"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VFU96_04825"
     CDS             complement(<1..468)
                     /locus_tag="VFU96_04825"
                     /inference="COORDINATES: protein motif:HMM:NF025022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEU5112618.1"
                     /translation="MTSRQTDQVATYVLDTCVLLADPQSPLRFDEHDVVLPLVVVEEL
                     DRKKTRMDEVGGNARAAIRLLEELGASRTGGLRDAVKLPGGGTLRIELNGIASERLPV
                     VLDPHTPDHRILATCLNLHEQTPAVLVTKDASLRIKGAQLGVPVQDYRADTVPV"
     gene            complement(531..947)
                     /locus_tag="VFU96_04830"
     CDS             complement(531..947)
                     /locus_tag="VFU96_04830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEU5112619.1"
                     /translation="MAKEYKVTRNVNRLMAWAARRGMGKTQLMTTTGRKSGQKREVPV
                     SPLVLDGAEYLVSPYGEVSWVHNVRANPLVTLRHGSKERQVRLEEMTGPTAASAAAAY
                     YERESFARPYMDVPENPSAADFAAKSALFPVFKVNV"
     gene            complement(965..>2545)
                     /gene="polX"
                     /locus_tag="VFU96_04835"
     CDS             complement(965..>2545)
                     /gene="polX"
                     /locus_tag="VFU96_04835"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEU5112620.1"
                     /translation="LGKKELTEIKGVGASTADKILEYMSTGSVAKLVSLREKYPPAFV
                     ELTKIPGLGPKTLKLIREQLGVEDIEGLRRAIAAEELRDLPGLGKTSEEKIGKAIERL
                     GLHGKDRRTPLVEALPLARSLASRIAAVDGVEAAVPCGSIRRFSDTVGDIDIVVATTD
                     AAVVSEFVVGLPEAAEVIGSGGTKTSFLTREGMQVDVRTVEPGQLGSALLYFTGSKAH
                     NIALRQRAIDRGWLLSEYGLFEEERPIASETEEVIYKALEMELVPPPLREGTGEVEAA
                     AAGRLPQLIELSQIKGDLHYHSDRSGDGRSTLEEMIEAAMARGYEYVAFTDHGEDLAI
                     NGSPREVMLEHRDRIHDLQRKYPDIRLFFGCELNIGPDGDLDYDADFRLEFDYCVASI
                     HSHFDLSQDRQTARILGALRDPAVNAIGHLSGRYVGRRPGVELDIDVILEGLAVTGVA
                     LEINGALDRLDATSEVARRAMARGVDFVIDTDSHHTSDLVRMGYGVTYAQRGWVTTDR
                     VVNARPIDEFLEWIARRR"
BASE COUNT          436 a          827 c          804 g          478 t
ORIGIN      
        1 gaccggcacc gtgtcggctc gatagtcctg taccggaacc ccgagctgtg cccccttgat
       61 ccgcaaggat gcatccttgg tgacgagcac cgccggcgtc tgctcgtgca ggttcagaca
      121 cgtggccagg atccggtgat ccggggtatg gggatcgagc accaccggca gacgctcgga
      181 ggctatgccg ttgagctcga tcctcagcgt gccgcctccg gggagcttga ccgcgtctcg
      241 tagaccaccg gtccgggagg cccccagctc ttccaggagc cggatggctg ccctggcgtt
      301 cccgccgact tcgtccatgc gggtcttctt ccgatccagc tcctcgacca ccaccagggg
      361 cagcaccacg tcgtgctcat cgaatctgag gggagattgg gggtcggcga ggagcacgca
      421 ggtgtccagg acgtaggtgg ccacctggtc ggtctgtctg gaggtcatcg ccaaggacca
      481 cagcttcctg ctccgcgcga tgaattacaa ggatcgtcga tgaacctggc tcagacgttc
      541 accttgaaca cagggaaaag cgccgacttc gccgcgaagt cggccgcgga gggattctcc
      601 ggcacatcca tgtagggacg ggcgaagctc tcccgctcgt aatacgcggc tgctgccgac
      661 gccgctgtgg ggccggtcat ctcttcgagc cggacctgac gttccttcga tccgtgacgg
      721 agggtgacga gggggttggc ccgaacgttg tgcacccatg acacctcgcc gtagggcgag
      781 accaggtact cggccccatc gagcacgagc gggctaaccg ggacttctcg cttctggccg
      841 ctcttccgcc ctgtagtcgt catgagctgg gtctttccca tcccccgccg ggcagcccac
      901 gccatgagtc gattcacgtt ccgggtcacc ttgtattcct tggccatggc cgagaagcta
      961 cccgtcatcg tctccgagcg atccactcca ggaattcgtc gatcgggcgg gcgttgacca
     1021 ctcgatcggt cgtcacccaa ccgcgctggg cgtaggtgac cccgtagccc atccggacaa
     1081 ggtcgctggt gtgatgcgag tcggtgtcga tgacgaagtc gacaccacgt gccatcgctc
     1141 gacgcgccac ttcggatgtt gcatccagcc gatcgagggc tccattgatc tcgagtgcca
     1201 cgccggtcac tgcgagcccc tccagtatca cgtcgatatc cagctcgaca cccgggcgac
     1261 gcccgacata tcggccgctg agatgcccga tggcattcac cgccggatcg cggagggcgc
     1321 cgaggatccg agcggtctga cgatcttgcg acaggtcgaa atgggaatgg atcgaggcga
     1381 cgcagtagtc gaactcgagg cggaagtcgg cgtcgtagtc gagatcgccg tcggggccga
     1441 tgttgagctc gcaaccaaag aagaggcgaa tgtcgggata ttttcgctgg agatcatgta
     1501 tccgatcgcg gtgctcgagc atcacctcgc gcggcgaccc gttgatggcc agatcttcac
     1561 cgtggtcggt gaacgccaca tactcatatc cccgcgccat cgccgcttcg atcatctctt
     1621 cgagcgtcga gcggccatcg ccggaccggt ctgagtgata gtggagatcg cccttgatct
     1681 gggagagctc gatgagctgc gggagcctcc ccgcggcagc ggcctccact tcgccggttc
     1741 cttcgcgcag aggcggcggt accagctcca tctccagcgc cttgtagatc acttcctcgg
     1801 tctccgacgc gatcggcctc tcctcctcga agagcccgta ttcgctgaga agccaacccc
     1861 ggtcgatcgc gcgctggcgg agggcgatgt tgtgggcctt cgaccctgtg aaatagagga
     1921 gggcggagcc gagctgaccg ggctcgacgg tgcggacatc gacctgcatg ccctcccggg
     1981 tcaggaagga ggtcttggtc ccgccggacc cgatcacctc ggctgcctcg ggtagaccga
     2041 cgacgaactc ggagacgaca gcggcatcgg tggtggcaac cacgatgtcg atgtccccga
     2101 ccgtgtccga gaatcgtcgg atggagccgc aaggcacggc ggcttcgaca ccatctacgg
     2161 cggcgatccg tgatgccaag gatctcgcca ggggcaacgc ctcgaccaag ggggtccgcc
     2221 ggtcctttcc gtggaggcca agacgttcga tcgctttgcc gatcttctcc tcggacgttt
     2281 tccccaatcc gggaaggtcg cgcaactcct cggcggcgat ggcgcggcgc aggccctcga
     2341 tgtcctcgac accgagctgc tctcggatga gcttgagcgt cttcgggccc agcccaggga
     2401 tcttcgtgag ctcgacgaat gcaggcggat acttctcacg aagggatacc aacttggcca
     2461 ccgagccggt ggacatgtac tcgaggatct tgtctgccgt cgaggcgccg actcccttga
     2521 tctcggtcag ctcctttttg cccag
//
LOCUS       DASTXO010000231         2507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_530174, whole genome shotgun sequence.
ACCESSION   DASTXO010000231 DASTXO010000000
VERSION     DASTXO010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530174"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1297)
                     /locus_tag="VFU96_04840"
     CDS             complement(83..1297)
                     /locus_tag="VFU96_04840"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5112621.1"
                     /translation="MTRSLAFVRKELVSVWRQPRLIATLVLGPFLILFLFGLGYQETP
                     EPFRTVLVTEQTEEAIGVDAEALDEAFGEGIDLVDVTDDVEAARQQLVDGEIDLVLVA
                     PEDGLEALQRGEQATFSILHSEIDPVITGSIDLLARLSVNQINQEVLTAVVEQARIEL
                     SQTEGPLADALNEAPEADPSLLVNPFTVETSPVTEPPTSQAAYYAPGVLILLVQHLSL
                     TFAALSLVRERELGITDVFRVSPLTVREAMSGKYLSFLVLGLTLATGLALTMPLLGVV
                     VQGPLWSFALTVSLVIFASLGLGFAISGLAKTDSQAVQYSMVVLLVSIFFTGFVLPLD
                     QLSVPVRWVSYLVPATFGISGLHDVIFRGQPAGPVVTGVLLLYGLVMVALAWLAVRRD
                     VQPASAPAVKVS"
     gene            complement(1316..2302)
                     /locus_tag="VFU96_04845"
     CDS             complement(1316..2302)
                     /locus_tag="VFU96_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5112622.1"
                     /translation="MPTEPVIIASDLTKRFEEDAGVFDLDLEVEKGRIVALIGPSGSG
                     KTTTVRLMTGLLAPDSGSVSVLGQDPTRFTSQTRNLIGYMAQDSVFFPDLTLRENLNF
                     SASLFGVPLRRKSRVNDIVEMLDLGGPIDRLLRDASGGEKRRLSLAAALIHRPELLFL
                     DEPTAGIDPILRKKFWDHFGHLAVTGSTLMVTTQYVGEAAYCDLVGVLAGGRILALDT
                     PEGLRRRAFGGDLFDVVFVAPPARDDLVALTRADGVSKVTWLDDHSVRLVVADAGEVS
                     STLDEWASERGLEMERSEAHLPPFDDVFVELVAAADDDHADQDSDDEVVLTG"
BASE COUNT          528 a          793 c          772 g          414 t
ORIGIN      
        1 agcaactgga cgagatcgag gtcaacgagg acgtccgcaa tgcgtgggac gagcttcgga
       61 ccaacctcga gcagttgatg aactagctga ccttcacggc tggggcgctc gccggctgaa
      121 cgtccctgcg gaccgcgagc caggcgagcg ccaccatcac caacccatag agcaggagaa
      181 ctccggtaac caccggcccg gcgggttgtc cccggaagat cacgtcgtgc agcccgctga
      241 tcccgaaggt cgcggggacc agatacgaaa cccaacgaac cgggacgctg agttggtcga
      301 ggggcaggac gaagccggtg aagaaaatcg acacgagcag caccaccatc gagtactgaa
      361 cggcctggct gtcggtcttg gccagacccg agatggcgaa gcccaggccc aacgaggcaa
      421 agatcaccag agagaccgtt agcgcaaagg accacagggg cccctgaacc acgaccccga
      481 gcaggggcat tgtcaatgcg aggccggtgg cgagggtcaa gccgagcacc aggaacgata
      541 gatacttgcc cgacatcgcc tctctgacgg tcaacggtga cacccgaaac acgtcggtga
      601 tccccaactc gcgctcgcgc accagagaca gagcagcgaa ggtgagcgag aggtgctgga
      661 cgagcagaat caagactccg ggcgcgtaat acgcagcttg tgacgttggc ggctccgtca
      721 ccgggctggt ctcgaccgtg aagggattta ccagcaggct cggatcggcc tccggcgcct
      781 cgttcagggc atcggccaac gggccctcgg tctggctcag ctcgatgcgg gcttgctcca
      841 ccaccgcggt gagcacttcc tgattgatct gattgaccga gagacgggcc agcagatcga
      901 tgctgcccgt gatgaccgga tcgatctcgg agtgcaggat cgagaaggtg gcctgctcgc
      961 cccgctgcag cgcctcgagc ccgtcctcgg gagccacgag gacgagatcg atctctccgt
     1021 ctacaagctg ttgtcgggcc gcctcgacgt cgtctgtcac gtcgaccagg tcgatgccct
     1081 caccgaacgc ctcgtcgagc gcttcggcat cgacaccgat cgcttcttcg gtctgttcgg
     1141 tcaccaggac cgtccgaaag ggctccggcg tctcctggta gccgagaccg aaaaggaaga
     1201 ggatcaggaa cggccccagg accagagtcg caatcaggcg gggctgtcgc cacacggaaa
     1261 ccagctcttt gcgaacgaac gcgaggcttc gggtcaagga tctcatggag tcgattcagc
     1321 ccgtcaggac gacctcgtcg tctgaatcct gatccgcgtg gtcgtcgtcg gcggcggcca
     1381 ccaattcgac gaacacatcg tcgaaaggtg gaaggtgagc ctccgacctc tccatttcca
     1441 ggccccgctc gctcgcccat tcgtcgaggg tggaggacac ctccccggca tctgcgacga
     1501 ccagacgcac actgtgatcg tcgagccagg tcaccttgct gactccatcc gcccgggtga
     1561 gggcaaccag atcgtcacgg gctggaggag cgacgaagac cacatcgaac agatcgccac
     1621 caaacgcgcg acggcgcagg ccttccggcg tgtccaaggc caggatccgg cctcccgcga
     1681 gcacaccaac caggtcgcaa taggccgctt cacccacgta ttgcgtcgtc accatcagag
     1741 tcgatcccgt tacggcgagg tgaccgaagt ggtcccagaa cttcttgcgc aggatgggat
     1801 cgatcccggc ggtgggctcg tctaggaaca gcagctcagg ccgatggatc agcgccgccg
     1861 ccaacgacag ccttctcttc tcaccaccgg aggcgtcgcg aagcagccgg tctatcggcc
     1921 cgccgagatc gagcatttcc acgatgtcgt tcacgcggct cttcctccga aggggcacac
     1981 cgaacagcga cgccgagaaa ttgagattct cccgaagggt caggtcgggg aagaagaccg
     2041 agtcctgagc catgtacccg atgagattcc gggtttgcga cgtgaatcgc gttggatctt
     2101 gacccaggac ggagaccgat ccggagtcgg gagcgagcag gcccgtcatc agccttacgg
     2161 tggtggtctt ccccgatcca ctggggccga tcaaggccac aattcgtccc ttttccacct
     2221 cgaggtcgag atcgaaaacc ccagcgtctt cttcgaagcg cttggtcagg tcggaggcga
     2281 tgattacggg ctcggtcggc atgggcgccg ccacttaccc gtacctagat ccccccaacc
     2341 accgccttgt gttgcgtttt gcgacgcgag ccaacgggta agtggtcctg acgaaaagga
     2401 ggctggcttg ggacgaagcg gcatcatctg gacgatcgtg ggcgtactgc tcatcatcgc
     2461 cctgctgatc tacatattct gaaatcaatt gctgagatca aatcgag
//
LOCUS       DASTXO010000232         2266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1414007, whole genome shotgun sequence.
ACCESSION   DASTXO010000232 DASTXO010000000
VERSION     DASTXO010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414007"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VFU96_04850"
     CDS             <1..437
                     /locus_tag="VFU96_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007105599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112623.1"
                     /translation="GHRPPAQRHQLRRGVNAGQMLRAGGIDSVRVRLLIHPVDPESIR
                     VRPAPGLMRRLWGRGISGMTLGKTIFLDPDLMVAAQHLPGLLLVHELVHVRQWGELGV
                     IRFLWRYLSAYVGGRWKGLGHRAAYLAIPLEVEARVAARLLE"
     gene            complement(447..1256)
                     /locus_tag="VFU96_04855"
     CDS             complement(447..1256)
                     /locus_tag="VFU96_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HEU5112624.1"
                     /translation="MLDIKPYLVAPGTEANLADRDPNTKQGFDGDKVEGKAELKPMSA
                     RLAELQTRLWAESKQKLLIVLQAMDTGGKDGTIRNVFEGVNPQGVRVWGFGVPTDWEL
                     AHDYLWRIHQRTPENGGITIFNRSHYEDVLVVRVKGLVPEPRWRKRYGHIVDFERLLV
                     DEGTTVVKLFLHISKDEQKKRLEARLEEHDKRWKFNVDDLADRKLWDDYADAYEEALT
                     TTSSEHAPWYVVPANRKWYRNLVVASIVIHHLVEMDPQYPPEPDLEGVVVE"
     gene            1340..1894
                     /locus_tag="VFU96_04860"
     CDS             1340..1894
                     /locus_tag="VFU96_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017822401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEU5112625.1"
                     /translation="MGRTQAMALGLVTAAVLVGAGLGALGGRAPIESATSMSVVAPEE
                     AAGFPVYVSGWVATPGVVEMVEGAIVADAVAAAGGALEGALLDEVNLARPVVEGDHLQ
                     IPGPGESMTAAAEGEAGDGLIHVNQADASVLEDLPGVGPVLAERIVAHREANGPFETV
                     EDLLEVPGIGEAKLAAMRDSISVP"
     gene            2038..>2266
                     /locus_tag="VFU96_04865"
     CDS             2038..>2266
                     /locus_tag="VFU96_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112626.1"
                     /translation="MRGRQLTALIVAIAAIHSAILASPVDPLPPGRIETEGVMAGDIE
                     DGRFGPFALIDLGSGPVLADLPPSADASRGDV"
BASE COUNT          341 a          712 c          752 g          461 t
ORIGIN      
        1 aaggtcatcg cccgcccgcc cagcgtcatc aacttcgtcg tggggtgaac gcgggacaaa
       61 tgctccgtgc gggtgggatc gactctgtcc gggtgcggct gctcatccat cccgtcgacc
      121 cggagtccat tcgggtccga cctgcgccgg ggctgatgcg ccggctgtgg ggccggggca
      181 taagtggcat gaccttgggc aagacgatat tcctcgaccc ggatctgatg gtggccgctc
      241 agcacctgcc gggcctgctc ctggtccacg agctggttca tgtccggcag tggggcgagc
      301 tgggtgtcat ccgctttctc tggagatatc tgtccgccta tgtcggcggg cgatggaagg
      361 gcctggggca tcgtgcggca tatctggcga taccactcga ggtggaggcg cgcgtggcag
      421 cccgtctcct cgagtgagga cagggactat tcgacgacca cgccctccag gtcgggctcg
      481 ggcgggtact gaggatccat ctccacgagg tgatggatca cgatgctggc caccaccaga
      541 ttgcgatacc acttccgatt ggcggggacg acgtaccacg gggcatgctc actcgatgtc
      601 gtggtgagtg cctcttcgta ggcatcggca tagtcatccc acagtttgcg gtccgccaga
      661 tcatcgacgt tgaacttcca acgcttgtcg tgttcctcga gacgggcttc gagtcgcttc
      721 ttctgttcat ccttggagat gtggaggaac agcttgacca cggtggtccc ctcgtcgacc
      781 aggagccgtt cgaaatcgac gatgtggcca taccgcttcc gccagcgtgg ctcggggacg
      841 agccccttta cccgcaccac gagcacatcc tcgtagtgcg agcgattgaa gatcgttatg
      901 ccgccgttct cgggtgttcg ctgatggatg cgccagaggt aatcgtgggc aagctcccag
      961 tctgttggga cgccgaagcc ccagaccctc accccctgag ggttgacacc ctcgaacacg
     1021 ttccggatcg tcccgtcctt gcccccggta tccatggcct ggagcacgat cagcagcttc
     1081 tgcttcgatt cggcccagag ccgtgtctgc aattcggcca gcctcgcgct catcggcttc
     1141 agctctgcct tgccctcgac tttgtcgccg tcgaaacctt gcttcgtgtt ggggtcgcga
     1201 tctgcgagat tcgcctctgt tcctggcgcg acgaggtatg gcttgatgtc gagcatgtgg
     1261 gcgagactat cgaacgacct tcttcactca tccgctctcg attggtatgt agaccggagg
     1321 agagcgtggt ggggtcggta tgggacggac acaggcgatg gcgctggggt tggtcaccgc
     1381 cgccgtcctc gtgggggccg ggctcggagc cctcggaggc cgggcgccga ttgaatcggc
     1441 aacttcgatg tcggtagtgg cgccggagga agccgctggc ttccccgtgt acgtctccgg
     1501 gtgggtcgcc acacctggcg tggtcgagat ggtcgaaggg gcaatcgtcg ccgacgcggt
     1561 cgccgctgcc ggaggtgccc tcgagggggc attgctcgac gaggtcaatc tggccagacc
     1621 ggtggtcgaa ggggaccatc tgcagatccc cggccccgga gaatcgatga ccgcggccgc
     1681 ggagggagag gcgggggatg gcttgatcca cgtcaaccag gccgacgcgt cggtcttgga
     1741 ggatcttccc ggcgtcggtc ctgtgctggc cgagcgcatc gtcgcccatc gcgaagccaa
     1801 cgggcccttc gaaaccgtag aggacctcct ggaggtgcca ggaatcgggg aggccaagtt
     1861 ggccgcgatg agagactcga tatccgtgcc ttgatctccg atcacccagc gttccctccg
     1921 cctgtctccc gaccctggat tggctgcgcg gcgtgggtga tcctcgcggc ttggggagcc
     1981 gggttctccc cgggctgggg tctactcctg gcgtcgatcc ccgttctgtg gatgtcgatg
     2041 agaggtcggc agctgaccgc cctgatcgtg gcgatcgccg ccatccactc cgcgattctg
     2101 gcttcccccg tcgacccatt gcccccaggt cgcatcgaaa cggagggggt gatggccggc
     2161 gacatcgaag acggccggtt tggcccgttt gccctcatcg acctgggttc gggccccgtc
     2221 ctcgccgatc tccctccgag tgccgacgcc tcccgtggtg acgtcg
//
LOCUS       DASTXO010000233        10352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1524561, whole genome shotgun sequence.
ACCESSION   DASTXO010000233 DASTXO010000000
VERSION     DASTXO010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 10352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10352
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524561"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VFU96_04870"
     CDS             complement(<1..863)
                     /locus_tag="VFU96_04870"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU5112627.1"
                     /translation="MPDKVTFEDPAGEGPAIILERFDVPDDVPDAELRHRKIPDPDPH
                     YVDLGMLYRFAQLEHARASGAEGLVPLHVAVRGHMGTGKDHDIEQFAAAMGLPYFRIP
                     LTGEVRDVTLIGSTRLHGDGKGGTESRWEDGDITRALRGPALLNLSELNAAGAETLFA
                     LHWLLDRYSSLDLPTGETVRLRPDVRIFGTMNPTDLRDYAGTQTLNKAFADRWVIWEK
                     NFPGGEQVEAMLTRRYPRLKEEYVTAIARLAVEVNASFEAQDSGVRVETPLSLRSVLD
                     RLPMGLVVHSDR"
     gene            complement(856..2937)
                     /locus_tag="VFU96_04875"
     CDS             complement(856..2937)
                     /locus_tag="VFU96_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112628.1"
                     /translation="MRGRDQTFLTDLIGPEDQAIEAVVSRYQRVAPAVTRFARTLSNN
                     PNLRVRLGSEAAASADEVVCDPRIFQAAYHRNAPVTPDEVAIASALHEVMHLVSTNLD
                     ESRELPDGWPIEGEIAPDAKVDLLTALERTNKPVAEVLFFSLEDARQEAQGLAAYPGA
                     RSVLADLYMSSLSQAISRSGALSQFVLGCFLLTGGYIERDVLEKRFESRATAAIDDAS
                     HHCDVAADSDDPWEVAQLALELEAIARAHGLITEVQANDTHAKRQHAAQSDAEKAKDG
                     VDAVRMTSPILTDADSYQDTRRSSEARSGMSDRKGASDIADEESTDQLLRVSQAPDIF
                     LPTGQGGKLVVGPIPSSFARFSNEGADALVRTAQRWGLAQRRVSGELFPLFAANQRRG
                     LRSGFDQGDVSPHAALFIGAGLYQRLYERRAARTRRTYAVSLLVDASASMLSPGKAGS
                     SWAMPAAMLGAWTMARLCDELQIDFEVALFNRGFAARPDDTEDTYRRKRSAAIAGLRR
                     TQGTAADRLTSTVNHYVVKPFERRWRDAEPTLAGLFYTAAEPRSAATLARRDPKTAPP
                     VSLFEKAANVDEFNIIHAAERMARLGAQVRVLMVLADGMTRGSLEALAKSVAAVESSG
                     TTVIGIGIGDHTVDEAYRRHEVVSQPEDLTQAMINGTRNALRRSLALWGMDTWWLRAS
                     EIKTYQETAVA"
     gene            3019..4272
                     /locus_tag="VFU96_04880"
     CDS             3019..4272
                     /locus_tag="VFU96_04880"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU5112629.1"
                     /translation="MHYDLTTLPNGLRVITEPMPSLRSVALGCWVDTGTRDELPNEQG
                     ASHFLEHLLFKGSEKLSARQVSEIFDAIGAESNAFTSKENTCYWVRLLDQDLGTGFDV
                     LSEMLQRPAFRQNEIDAERQVVIEEINMNDDDPDDVAFENFTMAVFVDHPLEAPVLGT
                     RDSIRAMSRDDILGYWKRRYGAGSMVVAAAGSIDHEAVVEMVAERFGDWSGETVEHEH
                     GEAAPWPTVKITHRETEQAHIVIGGKGLTRSDERRWAFEVLNHVMGSGMSSRLFREIR
                     EERGLAYAVYGFKMAYADAGAWGVYVGTTPSQTETAMKVIREELDKVVEEGITVEELE
                     RAKGSMRGGLALAMEDPNSRMVRLGRDELAGMPHLSVDERLAKLEAVTLEEVQAIASD
                     MYRGQRMIGAVGPYEEGELDQYLAV"
     gene            complement(4273..4614)
                     /locus_tag="VFU96_04885"
     CDS             complement(4273..4614)
                     /locus_tag="VFU96_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112630.1"
                     /translation="MLRVRNLAIFAAALLLTAASLGATAFFTADGPGSDLRPVGGAEE
                     TIQILQGNRSVLWTALSGSVGLYRVEGWPVSIDGNELGGALVIERPTDGSAGVIEFGE
                     KPKSIVFDVEG"
     gene            complement(4656..4988)
                     /locus_tag="VFU96_04890"
     CDS             complement(4656..4988)
                     /locus_tag="VFU96_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112631.1"
                     /translation="MSTIFLLAFGIVFFATGLTLINREVCEGICETLGLTLLYAGGPV
                     SALIGIFSDSVIVAWPLDVIVWVVLGFGVARVATNRRIQPWGLLVALLVFFLIYGLVL
                     SQLVELTA"
     gene            5106..5561
                     /locus_tag="VFU96_04895"
     CDS             5106..5561
                     /locus_tag="VFU96_04895"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEU5112632.1"
                     /translation="MELRDILLVLHIAGAGTWLGANIVQAMAPGMAAKQGPAAAAGWW
                     RIAAGLSTRLYMPAAIVLLATGVWMVLIDEVYGFNTLFVTIGFAMIVVGTLLGIFVFE
                     RSSMRAADAIDAGDQAGAKALRGRLAGFGILDTVLLLFTITAMVLRLGA"
     gene            5580..6830
                     /locus_tag="VFU96_04900"
     CDS             5580..6830
                     /locus_tag="VFU96_04900"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEU5112633.1"
                     /translation="MIRRSILFSLLLLIGGCTPGVDGAGVPEGGDVAVTTVPGSQLLG
                     KVTDSSGMPVPRATVTVGSDVAVTAPDGWFDLLTTPGALHVAKPAWVGMESAWDGSDG
                     FTQLAIEPRRIRALRVGAEAAGDDERFAAMLAMADETAINAFVFDTKQEGGKVFYETG
                     VTEAHDSGAVVNVYDPAQRIAQAKEHGLYTITRIVTFEDSYRAEFRPDEAYDGKWINP
                     TNPAAWEYPIALGEEACRLGFDEVQFDYVRFPSEDAAQVSGQLEMTEEERTSVIEGFL
                     REAQKRIHAAGCAVSADVFAIVVSASNDQGIGQKPEELTRHLDAFSPMVYPSHYSPGW
                     LGFDDPNEHPYDVTSDAIQDAQERIEPGVVLRPWLQAFWWTNDQIRTSIQAAEDHGVG
                     WILWNAVSNFDRPAIPTDAELAAT"
     gene            complement(6831..8537)
                     /locus_tag="VFU96_04905"
     CDS             complement(6831..8537)
                     /locus_tag="VFU96_04905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011602756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEU5112634.1"
                     /translation="MVRNPDTAPSSLNPAQRQAHLRRMGQDRFDVVVIGGGVTGCGVA
                     LDAASRGLSVALVEQRDYSSGTSSRSSKLFHGGLRYLEHRNFSLVREALGERNLMLNR
                     LCPHLARPVSFLYPLQHRAWERLYVGAGVLLYDLFASAADSPLPRHSHLSRKSALEVA
                     PALARSALVGAVRYWDAVVDDARHTMMLARTAAAHGALVASSVRARSLILDAGRVSGI
                     AATDLEEGRDLRINARRVINATGVWSDDIQAMAGDRGLDVKASKGIHIVVPRDRIRSE
                     TGLILRTERSVLFVIPWNRHWILGTTDTPWDLRRAHPAACSNDIDYLLTWVNSVLADP
                     LVPADIVGVYAGLRPLLTGESDDTSRLSREHAVLVSATGLISIAGGKYTTYRIMARDA
                     VDAAVADLGGNVPESRTDEIPLVGARGWAEMMEDPSRIGLPDPERLLGRYGSEVGSLA
                     ALVESDPSLGMPLVDDVPYLRAEIAVAALEEGALHLDDVLTRRTRISIETSDRGLQAA
                     EEATRIIGPILGWDDAASAREIDHYRARVAAERESQGQPDDNTADAARMGAPDVRTGR
                     SE"
     gene            8545..9450
                     /locus_tag="VFU96_04910"
     CDS             8545..9450
                     /locus_tag="VFU96_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5112635.1"
                     /translation="MSGQMKVGIQLPEVEREVTWREVREIAITAEEVGFDSIWVGDHL
                     LFRDPVTGVRGPMEAWSMLAALGEATERALIGPLVASTSFHNPAMLAKKAATVDDISG
                     GRLVLGLGAGWNQAEYAAFGFPFDHRVSRFEEAFTIIRTLLRDGAIDHDGRFYTHREM
                     TLLPRARADLPLLIGSNGPRMLRIAAPHVDLWNSWYTGFDNDPAGIAPLLATVDEACR
                     QVGRNPAEIDRTAAVLVQLDRGRGRIAGSSERPEVMPITGSSSDIAEALARFEDAGIT
                     HVQVVLDPIDAVAVGAMGEVLTRLR"
     gene            9505..10215
                     /locus_tag="VFU96_04915"
     CDS             9505..10215
                     /locus_tag="VFU96_04915"
                     /inference="COORDINATES: protein motif:HMM:NF001911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEU5112636.1"
                     /translation="MANLRITRLGHGGVLYRSPGDSWIWVDRWSGAPNFAEEYRNPEK
                     VDVVAPTHCHFDHVGDDCADLVELAGMVGSTVIGSHEMSVWLGARGVESIGMNNGGTV
                     EVAGVKFTMVHADHTGGATLGTGSDQVTRDLGCWGWILEFEDGTVVYQSGDTDLFGDM
                     ALIGERFSPTIGVFPIGGHYTMGPRDAGRAADMSGVSTVIPIHYATFPILVGTPDELR
                     QHTAAEVVALEPGETWGE"
BASE COUNT         1854 a         3290 c         3433 g         1775 t
ORIGIN      
        1 cgatcggagt gaaccaccag ccccatgggc aggcgatcga gtaccgagcg cagtgacaac
       61 ggcgtctcca ccctgacccc cgagtcctgg gcctcgaagg aggcgttgac ctcgaccgca
      121 agtcgggcga tggccgtgac gtactcctct ttcaagcgcg ggtacctgcg ggtgagcatg
      181 gcctcgacct gttcgccacc cggaaagttc ttctcccaga tcacccagcg gtcggcgaat
      241 gccttgttga gggtttgagt gcccgcatag tcgcgcaggt cggtcgggtt catggtcccg
      301 aagatccgca cgtccggcct caaacgcacc gtctccccgg tggggaggtc cagcgatgag
      361 tagcggtcga gcaaccagtg gagggcgaag agagtctccg ccccggcagc gttcaactcc
      421 gacaggttga gcagggcggg gccgcgcagg gcgcgggtga tgtccccatc ctcccagcgg
      481 ctctcggtgc caccctttcc gtcgccatgg aggcgggtcg agccgatgag cgtgacatct
      541 cgcacctctc cggtcagggg gattcgaaaa taggggagcc ccatggccgc ggcgaactgc
      601 tcgatgtcgt ggtccttgcc ggtccccatg tgccctcgga ccgcgacatg aagtggcacg
      661 agcccctccg ctcccgatgc gcgagcgtgc tccagttgtg cgaaccggta gagcattccc
      721 aagtcgacgt agtgcgggtc cgggtcgggg atcttgcgat gcctcagctc ggcatcggga
      781 acgtcgtcgg gaacgtcgaa ccgctcgagg atgatggccg gcccttcgcc ggcagggtct
      841 tcgaatgtca ctttgtcagg caacggctgt ctcctggtag gtctttatct ctgacgctct
      901 cagccaccac gtatccatac cccaaagggc caggctccgg cggagcgcat tccgggttcc
      961 gttgatcatc gcttgggtca ggtcttctgg ctgcgacacc acctcatgcc ggcgataggc
     1021 ctcgtccacg gtgtgatccc cgatgccgat cccgatcacg gtggtcccgc tcgattcgac
     1081 ggcggccacc gacttggcca gggcctcgag tgacccccgg gtcatgccat cggcgagcac
     1141 catcaggacc cgcacctggg cgccgagccg cgccatgcgc tcggcggcat ggatgatgtt
     1201 gaactcgtca acattggcag ccttctcgaa cagggatacc ggtggtgccg tcttgggatc
     1261 gcgacgggcc agagtggccg cggatcttgg ctccgctgcg gtgtagaaga gcccagccag
     1321 ggttggctcg gcgtcccgcc agcgccgttc gaatggcttg accacataat ggttcacggt
     1381 cgaggtgagg cggtcggcgg cggtgccttg ggtccgccga agaccggcga tggcggccga
     1441 cctcttgcgg cgataggtgt cctcggtgtc gtcgggccgg gcggcgaatc cacggttgaa
     1501 gagagccacc tcgaagtcga tctgcagctc gtcgcagagt ctggccatgg tccaggcgcc
     1561 gagcatcgcc gccggcatcg cccacgagct ccccgccttc cccgggctca gcatcgacgc
     1621 cgaggcgtcg acgagcaacg agaccgcgta ggtccgccgg gttcgcgccg cccgccgctc
     1681 atacagccgc tgatagagcc cggctccgat gaagagggcg gcgtgcggcg acacgtcccc
     1741 ctgatcgaac ccggagcgga gcccgcgccg ctggttggcg gcgaacaaag gaaacagctc
     1801 tccagagacc cttcgctgag ccaggcccca gcgttgcgcg gttcgaacca gcgcgtcggc
     1861 accctcgttg gaaaaacggg cgaaagagga cggtatgggg cccacgacga gctttccgcc
     1921 ctgaccggtg ggtaggaaga tgtccggcgc ctgactgacc cgtagcagct ggtcggtcga
     1981 ctcctcatcg gcgatgtcag aggcaccttt ccggtctgac atcccggacc gagcctcact
     2041 cgatcgcctg gtgtcctggt atgagtcggc gtcggtgagg atcggcgaag tcatgcggac
     2101 cgcgtccacc ccatcctttg ccttctctgc atctgactgg gcggcgtgtt gtcgtttggc
     2161 gtgcgtgtcg ttggcctgga cctcggtgat caatccgtgg gcccgggcga tggcctccaa
     2221 ctcgagggct agctgggcca cctcccaggg gtcatcggaa tccgccgcca cgtcgcagtg
     2281 gtggctggcg tcgtcgatgg ccgccgtagc ccgggactcg aagcgcttct ccaggacatc
     2341 tcgttcgatg tatccacctg tcagcaggaa gcagccgagc acgaactggc tcaaggcccc
     2401 tgatcgggag atcgcctggc tcagcgatga catatagagg tcggcgagga ccgatcgtgc
     2461 cccggggtag gcggccagac cctgtgcctc ttgtcgggca tcttcgagag agaagaagag
     2521 gacctcggcc accggcttgt ttgtccgctc gagggcggtg agcagatcga ctttggcatc
     2581 cggggcgatc tcaccctcga tcggccaccc atcggggagc tccctgctct cgtcgagatt
     2641 ggtggagacc agatgcatca cctcgtgaag agccgatgca atcgccacct cgtcgggagt
     2701 caccggagcg ttgcggtggt aagccgcctg gaagatccgc gggtcacaca cgacctcgtc
     2761 tgccgaggcg gccgcctctg agccgagccg gaccctgaga ttcgggttgt tggacaaggt
     2821 cctcgcaaag cgggtgaccg ccggagcgac gcgctgataa cggctgacga cagcttcgat
     2881 cgcctggtcc tccggcccga tcaggtcggt gagaaacgtc tggtcgcgtc cgcgcatagc
     2941 cagtcattgt ctcacgggtg tcaagctcgg tcagaagacc cgcgaacgct atccgcgccg
     3001 gacccataca ctcccgacgt gcattacgac ctgaccacac tccccaacgg gctccgtgtc
     3061 atcaccgagc ccatgccctc cctccgctcg gttgccctcg gctgctgggt cgacaccggg
     3121 acgcgcgacg aattgcccaa cgagcagggc gcttcccact tcctcgagca cctcctcttc
     3181 aaaggttcgg agaagctgtc ggcacgtcag gtgtccgaga tcttcgacgc catcggagcg
     3241 gaatccaacg cgttcacatc caaggagaac acttgctatt gggtcaggct cctcgatcag
     3301 gacttgggga ccggattcga cgtcctctcc gagatgttgc agcgacccgc cttccgccaa
     3361 aacgagatcg acgccgagcg tcaggtggtc atcgaagaga tcaacatgaa cgacgacgac
     3421 cccgatgacg tcgctttcga gaacttcacc atggctgtct tcgtcgacca tccgctcgag
     3481 gccccggtct tgggaacgcg ggactcgata cgcgcgatgt cacgggacga catcctcggt
     3541 tactggaagc gccgctatgg ggcaggatcg atggtggtgg ccgcggccgg ttcgatcgac
     3601 catgaggctg tggtcgagat ggtggccgag cggttcggcg actggtcggg cgagaccgtc
     3661 gagcacgaac acggcgaggc cgccccgtgg ccgacggtca agatcacaca tcgagagacg
     3721 gagcaggccc acatcgtcat cggtggaaaa ggcctgaccc ggagtgacga gagacgctgg
     3781 gcgttcgagg tgctcaacca tgtcatgggc tctggcatgt cttcccgact cttccgggag
     3841 atacgggagg agcgggggct cgcctacgcc gtctacggct tcaagatggc ctatgccgac
     3901 gccggtgcgt ggggcgtcta tgtgggaacg acgccgagcc agaccgagac cgcgatgaaa
     3961 gtgattcggg aggagctcga caaggtcgtc gaggagggga tcaccgtcga agagctggag
     4021 cgcgccaagg gttcgatgcg cgggggcctg gccctcgcca tggaagatcc caacagccgg
     4081 atggtgagac tgggccggga cgaactggcg ggaatgccac atctgtctgt cgacgaacgg
     4141 cttgccaagc tcgaggcagt gacgctcgag gaagtccagg cgatcgcatc ggatatgtac
     4201 cgtggtcagc ggatgatcgg cgcggttggg ccctacgaag aaggtgagtt ggatcagtac
     4261 ctggcggtct gactaaccct cgacatcgaa gacgatcgac ttcggcttct ccccgaactc
     4321 gatgacaccc gccgagccat cggtcggccg ctcgatcacc aaggcgcccc ccagttcgtt
     4381 gccgtcgatg ctcaccggcc agccctccac ccggtagagg cccaccgagc cggacagggc
     4441 cgtccagagg acgctgcggt tgccctggag gatctgaatc gtctcctccg ccccgccgac
     4501 cgggcgcaga tccgagcccg gcccatcggc cgtgaagaat gcggtggctc ccagcgacgc
     4561 tgcagtcaag agaagggcag cggcgaatat ggcgagattc cttactcgga gcacacccat
     4621 cattgaagcc gggagcctcc cgacagcgga catttctacg cagtcaattc gactaactgc
     4681 gaaaggacta gtccgtagat caggaagaag accagcagcg ccaccagcag gccccagggc
     4741 tggatgcgcc ggttggtggc cactctcgcc actccaaagc cgagcacgac ccacacgatc
     4801 acatcgagcg gccaggccac gatgaccgaa tcggagaaga tcccgatcag cgccgatacc
     4861 gggccgcccg catagagaag tgtcaggccg agcgtctcac atatgccttc gcagacctca
     4921 cggttgatca gggtcagtcc tgtggcgaag aagacgatcc cgaacgcaag gaggaagatc
     4981 gtggacaagg cggcgtcccg acaacctatg ccttcagatc tgcctgccac ttgacggtcg
     5041 atggtaagcc cacgaagaag tcggcccgcc gattaacttt gcttcgaaga gcgaacggag
     5101 tcctgatgga attgcgtgac atcctgctcg tgctgcacat cgccggggcc gggacctggc
     5161 tcggggccaa catcgtccag gcgatggcac ccgggatggc cgccaagcaa ggcccggccg
     5221 ctgctgccgg atggtggcgc atcgccgcgg ggctttccac ccgtctctac atgccggcgg
     5281 ccatcgtcct gctggcaacc ggggtctgga tggtcctgat cgacgaggta tacggattca
     5341 acaccctctt cgtgaccatc ggcttcgcca tgatcgtcgt tgggacattg ctcggcatct
     5401 tcgtattcga gcggagctca atgcgggctg cggatgccat cgatgccggc gatcaagccg
     5461 gagcaaaggc tctccgggga cggttggcag gcttcgggat cctcgacacc gtgcttctcc
     5521 tattcacgat cacggccatg gtgctccgtc tcggagcctg acaggggctc ggcgggccat
     5581 tgatccggcg ctccatcctc ttctctctcc tcctgctgat tggtgggtgc acgcccggcg
     5641 tggatggcgc cggcgttccg gagggcggtg atgtcgccgt gaccaccgtc cccggatccc
     5701 agctcctggg gaaagtgacc gactcgtcgg ggatgccggt cccgcgggcg acggtgaccg
     5761 ttggctccga tgtcgcggtg accgccccag acggatggtt cgatcttctc accaccccgg
     5821 gcgctctcca tgtagccaaa ccggcgtggg tcgggatgga gtcggcatgg gacggatccg
     5881 atgggttcac tcagctggcg atcgagcccc ggcgcattcg agccctgagg gtcggcgccg
     5941 aagcagcggg ggacgacgag aggtttgccg ccatgctcgc catggccgat gagaccgcta
     6001 tcaacgcgtt cgtgttcgac accaagcagg aaggcggaaa ggtcttctac gaaaccggcg
     6061 tgacagaggc acacgactcc ggggcggttg tgaatgtcta tgacccagca cagcgaatcg
     6121 cccaggccaa ggagcacggg ctgtacacca tcacccgaat cgtcaccttc gaggactcgt
     6181 atcgcgccga attcagaccc gacgaggcct atgacggcaa atggatcaat ccgaccaatc
     6241 cggcggcatg ggaatacccc attgccctcg gcgaggaggc atgtcgcctc gggttcgacg
     6301 aagtccaatt cgattacgtc aggttcccgt cggaggacgc agcccaggtc agcggtcagt
     6361 tggagatgac cgaagaggag cggacctcgg tgatcgaggg attcctgcgc gaggcacaaa
     6421 agcgcatcca tgccgcgggc tgcgcggtca gcgcagacgt gtttgccatc gtcgtgtcgg
     6481 cgagcaacga ccaggggatc ggacaaaagc ctgaggagct gacccggcat ctcgacgcct
     6541 tcagccccat ggtgtatccc agtcactatt cgccgggctg gctcgggttc gacgatccca
     6601 acgagcaccc atacgacgtc acctcggacg cgattcagga tgcccaggag cgcatcgagc
     6661 ccggagtcgt cctccgtccg tggctacagg cattttggtg gaccaacgat cagatcagga
     6721 cgtcgatcca ggccgccgag gaccacggcg tcggctggat tctgtggaat gcggtcagca
     6781 acttcgacag gccggcgatt cccaccgacg ccgagctggc cgccacctga tcactcgctg
     6841 cgccctgtcc gcacatcggg agctcccatg cgggcggcgt cggcggtgtt gtcgtcgggt
     6901 tggccttgag attcacgttc agcagccacc cgggcccggt agtgatcgat ctcgcgtgcc
     6961 gaggctgcgt cgtcccagcc gaggatgggc ccgatgatcc gggtggcctc ttctgcagcc
     7021 tgcaggccgc gatcgctggt ctcgatcgag atcctggtgc ggcgggtgag gacgtcgtcg
     7081 agatgaaggg caccctcctc caaggcggcg acggcgatct cggcgcggag ataagggaca
     7141 tcgtcgacca gaggcatgcc cagcgaagga tcggactcga cgagcgcggc gaggctgccc
     7201 acctcggagc cgtaacgccc gagaagacgc tcagggtcgg gaagaccgat acgtgacggg
     7261 tcctccatca tctcggccca accccgcgcc ccgaccaatg ggatctcgtc agtgcgggac
     7321 tcgggcacgt tccctcccag atccgcgacg gccgcatcga cggcgtcgcg agccatgatc
     7381 cgataggtgg tgtacttgcc tccggcgatg ctgatgagcc cggtagccga gacgagcacg
     7441 gcgtgctcgc ggctgaggcg gctggtgtca tccgactcgc cggtgaggag agggcgaagt
     7501 ccggcgtaca cgccgactat gtccgccggg acgagcggat cggccagaac cgagttcacc
     7561 cacgtcaaca gatagtcgat gtcgttgctg caggcggccg ggtgggcacg tcgcaggtcc
     7621 cagggcgtgt cggtggtccc gaggatccaa tggcggttcc acgggatgac gaacaggacg
     7681 ctcctctctg tccgcaggat caacccggtc tcggagcgga tgcggtctct cgggacgacg
     7741 atgtggatcc ccttcgatgc tttcacgtcg agccctcgat cgcctgccat ggcctggatg
     7801 tcatcgctcc agacaccggt ggcgttgatc acgcgccgcg cattgatccg aaggtcccgt
     7861 ccctcttcca ggtcggtggc cgcgatgcct gatactcgac cggcatcgag gatgagagat
     7921 cgagctctga cgctgctggc gaccagggct ccatgggccg cggcggtccg ggcgagcatc
     7981 atcgtgtggc gggcgtcgtc gacgaccgcg tcccaatacc tgaccgcccc gaccagggcg
     8041 ctacgagcga gggccggagc cacttcgagc gctgacttgc gactcagatg tgaatgacgg
     8101 gggagagggc tgtcggcggc ggaggcgaac aagtcgtaca gaagcacccc ggccccgacg
     8161 tagaggcgct cccaagcccg gtgctgcaac ggatacagga acgaaaccgg tcgggcaagg
     8221 tgaggacaga ggcgattgag catcaggttc ctctcgccca gcgcctcacg caccaacgag
     8281 aagttcctgt gctccagata gcggagccca ccgtgaaaga gtttgctgga gcgactcgat
     8341 gtccccgagg agtagtcgcg ctgttcgacg agcgcgaccg agagcccgcg tgaggccgcg
     8401 tcgagggcga ccccgcaccc ggtgactccg ccaccgatga ccacgacgtc gaaccggtcc
     8461 tggcccatgc gtcgcagatg ggcctgtcgc tgcgccgggt tgagactgga aggggctgtg
     8521 tccgggttac gtaccatcgc atccatgagc ggtcagatga aggttggcat acagcttccc
     8581 gaagtagagc gcgaggtgac gtggcgggag gtgcgcgaga ttgcgatcac cgccgaggag
     8641 gtcggattcg actcgatatg ggtcggcgac catctcctgt tccgcgaccc ggtgaccggg
     8701 gtgcgcggcc ccatggaggc gtggtcgatg ttggccgcac tgggcgaggc caccgagcgg
     8761 gctctgatcg ggccgctcgt cgcctccacc tccttccaca atccggcgat gctcgccaag
     8821 aaggcggcga cggtcgatga catcagcggc ggacggctcg tgctcggcct cggcgccggg
     8881 tggaaccagg ctgagtacgc cgccttcggc ttccccttcg accatcgagt atcgagattc
     8941 gaagaggcgt tcaccatcat ccgaactctc cttcgcgacg gtgctatcga ccatgacggc
     9001 cgtttctaca cccatcggga gatgaccttg ctgcccaggg cacgggccga tttgccattg
     9061 ctgatcggct ccaatggccc ccggatgctc cggatcgccg ccccgcacgt cgatctgtgg
     9121 aacagctggt acaccggctt cgacaacgat cccgccggca tcgccccctt gttggcgacc
     9181 gtcgacgagg cctgccgcca ggtcggccga aacccggccg agatcgatcg cacagccgcc
     9241 gtcctggtcc agttggaccg gggacgcggt cgcatcgcag gcagtagcga acgtcccgaa
     9301 gtcatgccga tcaccggctc gtcgtcggat atcgccgagg cgctcgcccg tttcgaggac
     9361 gctgggatca cccacgtcca ggtggtcctc gacccgatcg atgccgtcgc agttggcgcg
     9421 atgggagagg tgctgacgcg gcttcgatga gtagcgtcgc agacctgatc gctgccggct
     9481 taggctgatc acgaaggaga gagcatggcc aatctgcgca tcacccggct cgggcacggc
     9541 ggggtcctct accggtcgcc gggcgattca tggatctggg tcgatcgttg gtcgggcgct
     9601 cccaacttcg ccgaggagta tcgaaacccg gagaaggtcg acgtggttgc accgacgcac
     9661 tgccatttcg accacgttgg ggacgattgc gcagatctgg tcgagctggc cgggatggtc
     9721 gggtccacgg tcatcggctc ccatgagatg tccgtttggc tcggggcccg gggtgtcgag
     9781 tcgattggga tgaacaacgg cggaacggtg gaggtggcgg gggtcaagtt cacgatggtc
     9841 cacgccgacc acaccggtgg cgcaacattg ggcaccggca gcgaccaggt gacccgggac
     9901 cttggatgct ggggttggat cctggagttc gaggacggca cggtcgtcta tcagtccggc
     9961 gacaccgatc tgttcggtga catggcgctg atcggcgagc gcttcagccc gaccatcggc
    10021 gtgttcccta tcggagggca ctacacgatg ggcccgcgcg atgccggcag ggcggcggac
    10081 atgtccggtg tgtctacggt catcccgatt cactacgcga cattccccat cctggtggga
    10141 acccccgacg agctccgcca gcacaccgca gccgaggtgg tcgccctgga accgggcgag
    10201 acatggggtg aatgaccgct aactgccggc gctcgaggcg acgagggctg gccgtcgaag
    10261 cgatctgagg atcagcacga cgaagaagag cacgatcggg atcactgcca tggtcgccga
    10321 gcccgatgtg ttggcgtgga aactgacgat ga
//
LOCUS       DASTXO010000234         6327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1598017, whole genome shotgun sequence.
ACCESSION   DASTXO010000234 DASTXO010000000
VERSION     DASTXO010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6327
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598017"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="VFU96_04920"
     CDS             complement(<1..932)
                     /locus_tag="VFU96_04920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEU5112637.1"
                     /translation="MGETSSDNSVDGPIGGFFEDLRRRVVSSATAPFRHAPYPLAHSL
                     DHRGDPGLFGPESVSWRVIGDAASFVGGIRALLIQAAHPEVVAGVGDHSRYREDPLGR
                     LSRTSAYVTATTYGAMPEVEQAVAQVRRIHRVVSGTSSRDIEYDADDPGFSAWVHNAL
                     TDSFLVANQTFGTDHLTTEESDRFVLEQTRVGALLGADPIPTTASALSRWIEGHPGLG
                     PSPEMSDAVDFLTDPPLEPGLKIGYKLLMEAAVATLPSRLRDILGVTKAPGAVRVGKG
                     VVAGLRWALGASPSWNLALVRMGAPVPGGRFKQP"
     gene            complement(910..1443)
                     /locus_tag="VFU96_04925"
     CDS             complement(910..1443)
                     /locus_tag="VFU96_04925"
                     /inference="COORDINATES: protein motif:HMM:NF039198.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HEU5112638.1"
                     /translation="MAGVQLALDLFLIFTLRLLDVGMATVRIVLISRGRRGPAALLGF
                     FEALVWVIAISRVLAGLNDPARMIAFALGFAAGTYAGSMVEEWLALGQSLIRVVAPVH
                     TDPVAPLLREQGYGATVLNGDGLSGEVRVTFCVVPRKDVWSVTHLIHEANPEAFVTVE
                     QTSTVDLAARHGGDVQR"
     gene            complement(1456..1686)
                     /locus_tag="VFU96_04930"
     CDS             complement(1456..1686)
                     /locus_tag="VFU96_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112639.1"
                     /translation="MTRARDPKPRTRSMGMGVALSNAMAELEVLLSPGAEHRIEEERK
                     VEIIRDQVGVVLDEHSGKVIVVEPGEISQEPG"
     gene            1782..3260
                     /locus_tag="VFU96_04935"
     CDS             1782..3260
                     /locus_tag="VFU96_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEU5112640.1"
                     /translation="MSAVESTDHAVVIAGGGPTGLMLAGELALSGVDVAIVERRATQD
                     LEGSRAGGLHARAIEVLDQRGIAERFLSEGQVHPFVGYAGTFLDISDFPTRHNYVLAL
                     WQNHIERILAGWVEELGAPILRSREVVGFAQDDTGVDVELSDGTSHRAEYLVGCDGGR
                     SLVRKAAGIDFPGWDPTASYLIAQVEMEDEPEIGMRPEGGGIGPVNREEGGGPYGVVL
                     LEKHIEHTSEPTLQDLRELLIAAYGTDYGVHDPIWISRVTDTTRQAVSYRARRVLLAG
                     DAAHVHGPQGGQGLNTGVQDAVNLGWKLAQVVNRTSSESLLDTYHAERHPVGARVLRN
                     TMAQVALARIDERSKALRDTMMEQLSMDEPRRHIAGMISGLDIHYDLGEGHPLLGRRM
                     PDLDLQTADGPTRVFALLHDARPVLLDLGEPGGFDITPWAARVQMVDATYLGTWELPV
                     LGEVAQPPPAVLIRPDGHVAWTGDLADPALPDVLTTWFGRDV"
     gene            complement(3257..4414)
                     /locus_tag="VFU96_04940"
     CDS             complement(3257..4414)
                     /locus_tag="VFU96_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112641.1"
                     /translation="MRRLTAFFACFALMGLAVAASAHETENYKDKFTSISWHGSDGSL
                     SWAGPWSEIGDDGDEKKGNVRVVSSGNCSGTCMRMSALTTLLGSIGAVRSADTSLLEG
                     LTLSFDIRSTSSLLASTLDVQVNDGGGWVQVAHYQLASGINEHASIDVSDFSSENFRV
                     RFLFSGLLLSSEVFIDTIEVLGTYTEPTTTTTTTVPQTTTTISPSTTTTHPATTTTTT
                     GPEGTTTTTTPGSGSPATTTTTDPGRENSTTTTVPGGAGGSTTTTAPDSTTTTTVVAG
                     GPGGRGPGGGATGGGPDDDAIDGESSAEAFASGGSGIRAAARGLQANFQGDLYGEVRS
                     VSALNGTDFRADFSLAVEVIEASWVWIVVLGLVIAYSILSGLDRHSALPDS"
     gene            complement(4411..5811)
                     /locus_tag="VFU96_04945"
     CDS             complement(4411..5811)
                     /locus_tag="VFU96_04945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5112642.1"
                     /translation="MGTERLTSPTARRRAVGIAIGATLVLVVLVVGLTFANSVGAARV
                     AGNAADLHWTNAALGNGALTRAAVVQATTFSDLAESELVTREDLSAAMGEAHDARDRL
                     AELLDAGGTSASSASLTHFLGRMDEAIADLEGGDFTSAKTILMTDAESAYNDLVESLQ
                     TEQIAIQAQIDANTAGSRRTTEYIQFFLMLAIPASAVLIYRFVARRQMRELRLKIDLE
                     LEKEREIGRAKDEFIAGLSHELRTPLTSIYGFAEILTDGGAEDSATAVEVSQIIANEA
                     AEMTRMVDDLLAVARLESTGVQIEMSPTHVTDVVDSAILPFERAGLKVKRAGTDALVS
                     TDAARLRHVLINLLSNAARHGGPNIGVEVSTGDGVVDIEVWDDGAGVPDDQLESLFDT
                     FIHNGAATLMTGTLGLGLGVASRLMDLLGGKLVYQRFAQKSYFIVSLPLLAVSAEIPK
                     TSDDATVAHLIRAMSA"
BASE COUNT         1116 a         2004 c         2102 g         1105 t
ORIGIN      
        1 aagggttgtt tgaagcggcc cccaggaacc ggcgccccca tccgcaccag tgccaggttc
       61 caggagggcg atgcacccag tgcccacctc aatcccgcca cgactccctt ccccactctc
      121 accgcccctg gtgccttggt cactcccagg atgtcccgca accggctggg cagggtggca
      181 acggctgcct ccatcaacag cttgtaaccg atcttgagac ccggttccag cggtgggtcg
      241 gtgaggaagt cgaccgcatc gctcatctcg ggcgatggac caagccctgg gtgcccttcg
      301 atccagcggg acagcgcgga ggcggtggtc ggtatcggat cggcgccgag gagggcgccg
      361 acacgggtct gctcgaggac gaaccggtcg ctctcctcgg tggtgaggtg atccgtcccg
      421 aaagtctggt tggcgactag gaaggaatcg gtcagtgcgt tgtgcaccca agccgagaac
      481 ccgggatcgt cggcgtcgta ttctatgtct cgactcgagg ttcccgacac cactcgatgg
      541 atgcgacgca cctgagccac tgcctgctcg acttcgggca tcgctccgta cgtcgtggca
      601 gtgacatagg cggaggtcct cgagagccgc cccagtgggt cttcgcgata tcgggagtgg
      661 tcgccaacac cggccaccac ttcgggatga gcggcctgga tgaggagggc gcggataccg
      721 cccacgaatg aggcggcgtc tccgatgacg cgccaggaca ccgattcggg accgaacagc
      781 cccgggtcgc cgcggtggtc gagactgtgg gccagcgggt agggcgcgtg tcgaaagggg
      841 gcggtcgccg aggagacgac gcgccggcgc agatcctcga agaagccacc aatgggcccg
      901 tcgaccgagt tatcgctgga cgtctccccc atgtctcgca gccagatcga cggtggatgt
      961 ctgctcgacg gtgacgaagg cttcgggatt ggcctcatgg atgagatggg tcacggacca
     1021 cacgtctttc cgggggacga cacagaaagt gaccctcacc tctccagaca gaccgtcacc
     1081 gttgagcacg gtcgcaccgt aaccctgttc ccggagcaac ggagcaacag gatcggtgtg
     1141 gacaggcgcg accacccgga tgagcgactg gcccagcgcc agccattcct cgaccataga
     1201 gccggcgtag gtgcctgcgg cgaaaccgag ggcgaacgcg atcattcgag cggggtcatt
     1261 cagacccgcg agcaccctcg agatggcaat gacccagacc agtgcctcga agaaacccag
     1321 gagtgccgcc ggcccgcgcc gtccccggct gatcaggacg atgcgcacgg tggccatgcc
     1381 gacgtcgagc agacggagag tgaatatcag gaacaggtcg agggcgagct gcacgcccgc
     1441 cacggtagga cgcggctacc cgggctcctg ggagatctct cccggctcga cgacgatgac
     1501 tttgccgctg tgctcgtcca ggaccacgcc tacttgatcc ctgatgatct cgaccttgcg
     1561 ctcctcttcg atccggtgct cggcgcccgg gctgagcagg acttcgagct cggccatcgc
     1621 attggagaga gccacaccca tccccatgga gcgtgttctt ggtttggggt cgcgcgcccg
     1681 ggtcatggcc agacgataac ttcgacccga cgcctaatcc cggtcagcaa gattcaggca
     1741 gcggcttgct cggggcgttg gacgggtagg aggaccgaat catgagtgct gtggaatcga
     1801 ccgaccatgc ggtggtcatc gcaggaggtg gaccaaccgg gctgatgctg gcgggcgagc
     1861 tggctctgtc gggcgtcgac gtcgccatcg tcgaaagacg cgccacccag gacctcgaag
     1921 gttcgcgggc cggtggtctt cacgcccgcg ccatcgaggt gctcgatcag aggggcatcg
     1981 ccgaacgctt cctctccgag ggccaagtgc atccgttcgt cggctacgcc gggaccttct
     2041 tggatatcag cgacttcccc acacgtcaca actacgtgct cgcgctgtgg cagaaccaca
     2101 tcgaacggat tctggctggg tgggtcgagg agctcggggc tccgatcctt cgcagccgcg
     2161 aggtggtcgg tttcgctcag gacgacaccg gcgtcgatgt cgaattgtcc gacggcacgt
     2221 cgcaccgggc cgagtacctc gtcgggtgcg atggagggcg cagcctggtc cgcaaggcag
     2281 ccggtatcga cttccctgga tgggacccga cagcgagcta cctgattgca caggtcgaga
     2341 tggaggacga gccggagatc ggaatgcgtc ccgagggggg cggcatcggt cccgtcaacc
     2401 gagaggaggg cgggggaccc tacggggtcg ttctgctcga gaagcacatc gaacacacca
     2461 gcgagcccac cctgcaggac ctccgcgagc tgctcatcgc cgcctacggg accgactacg
     2521 gggtgcacga ccccatctgg atctcccgcg tcaccgacac gacccggcag gcggtgtctt
     2581 atcgcgcgag acgtgtcttg ctggcaggcg acgccgccca tgtgcatggc ccgcaaggtg
     2641 ggcagggcct caataccggc gtgcaggatg ctgtgaacct gggttggaag ctggcccagg
     2701 tggtcaacag gacatcatcc gagagcctcc tggacaccta tcacgccgaa cgacatccgg
     2761 tcggtgcccg ggtgttgcgc aacacgatgg cccaggtcgc cctcgcccgc atcgacgagc
     2821 gcagcaaggc cctccgcgac acgatgatgg aacagctgag catggacgag ccgcgtaggc
     2881 acatcgccgg catgatctcc ggtctcgaca tccactacga cctcggggag gggcatccgc
     2941 tgctcgggcg gcgcatgccg gacctggatc tgcaaaccgc cgacggtccg acgcgggtgt
     3001 ttgccctgtt gcacgacgcc cgtcccgtgc tcctcgacct cggtgaaccc ggcggcttcg
     3061 acatcacccc atgggcggct cgcgtccaga tggtcgacgc gacatatctg gggacgtggg
     3121 agctaccggt ccttggagag gtcgctcagc ctcccccggc tgtgttgata cgacccgacg
     3181 ggcatgtcgc ctggaccgga gacctggcgg acccggcgct tcctgacgtg ctgaccacct
     3241 ggttcggacg cgacgtctag ctgtcgggga gtgccgaatg ccggtcgagg ccggagagga
     3301 tcgagtaggc gatgaccagc ccgaggacca cgatccagac ccacgacgcc tcgatcacct
     3361 cgactgcaag gctgaaatcg gcccggaagt cggtcccgtt cagcgcagag actgaccgca
     3421 cttctccgta gaggtcgccc tggaagttgg cctggagccc tcgagcggca gctcggatcc
     3481 cgctgccgcc cgaggcgaag gcctcggccg agctctcgcc atcgatggca tcgtcatcag
     3541 gcccgccccc ggtggccccg ccgcccgggc ctcgccctcc tggtccgccg gcaacgaccg
     3601 ttgttgtggt cgtcgagtcc ggggcggtcg tggtcgtgct cccgccggcg ccaccaggca
     3661 cggtcgtcgt cgttgagttc tcacgccccg ggtcggtcgt cgtggtcgtc gccggggatc
     3721 cagatccggg cgtcgtggtg gtcgtcgtgc cctctggtcc tgtggtggtg gtagtcgtgg
     3781 cggggtgtgt cgtcgtcgtc gacggagaga tggttgtcgt tgtctggggg acagtggttg
     3841 tcgtcgtcgt ggttggctcg gtgtacgtgc cgaggacctc gatggtgtcg atgaacacct
     3901 cgctgctcag aagcagcccg gagaagagga agcgaaccct gaagttctcc gagctgaagt
     3961 cgctcacgtc gatggaggcg tgctcgttga tgcccgacgc cagttggtag tgcgcgacct
     4021 ggacccagcc accaccgtcg ttgacctgga cgtcgagcgt ggaagcaaga agcgaagacg
     4081 tgctcctgat gtcgaagctg agggtcaacc cctcgagaag gctggtatcg gccgacctca
     4141 ccgcgccgat cgatccaagc agcgtcgtga gggcgctcat tcgcatgcag gtgccggagc
     4201 agttccccga cgacaccaca cgaacattgc ccttcttctc gtctccgtcg tcacctatct
     4261 cgctccatgg tcccgcccat gaaagagagc cgtcagagcc gtgccaactg atcgaggtga
     4321 acttgtcctt gtagttctcg gtctcgtggg ctgaggcggc gaccgccagg cccatcagag
     4381 cgaagcaggc gaagaatgcg gtcaggcgcc tcatgccgac atggcccgga tcaggtgggc
     4441 caccgtcgcg tcgtcagatg tcttcggtat ctccgccgag acggcgagga ggggcagcga
     4501 aacgatgaag tagctcttct gggcaaagcg ctggtagacg agcttgccgc ccagcaagtc
     4561 catcagccgt gaggccacgc cgagaccgag gccgagggtc cctgtcatga gagttgcggc
     4621 gccgttgtgg atgaaggtgt cgaagaggct ctccaactga tcgtcgggaa caccggcgcc
     4681 atcgtcccag acctcgatgt cgaccacacc atcaccggtc gacacctcga caccgatgtt
     4741 cgggccgccg tgtcgggcgg cattggagag gagatttatc aggacgtgac ggagccgggc
     4801 ggcatcggtg gacaccaatg catccgtgcc ggccctcttg accttgaggc cggcccgctc
     4861 gaagggaagg atcgccgagt cgacgacgtc ggtgacatgg gttgggctca tctcgatctg
     4921 cacgccggtg ctttcgagcc gggccacggc aagaagatcg tcgaccatgc gggtcatctc
     4981 ggcggcctcg ttggcgatga tctgggatac ctcgactgca gtcgccgagt cttctgcccc
     5041 accgtcggtc aggatctcgg cgaacccata gatggaggtg agaggggttc gcagctcatg
     5101 ggacagtccg gcgatgaact cgtccttggc cctcccgatc tccctttcct tctcgagctc
     5161 caggtcgatt ttgagacgga gctcccgcat ctggcgccgg gccacgaacc ggtagatgag
     5221 caccgccgaa gcggggattg cgagcatcag gaagaactgg atgtactcgg tggtgcggcg
     5281 agagccggcc gtgttggcgt cgatctgtgc ctggatcgcg atctgctcgg tctggagaga
     5341 ctcgaccaag tcgttgtagg ccgattcggc gtcggtcatc aaaatcgtct tggcactggt
     5401 gaagtcaccg ccctcgagat cggcgatcgc ctcgtccatc cggcccagga aatgggtcag
     5461 ggatgcgctg gaggcactgg tcccgcccgc atcgaggagc tcggccaatc ggtcccgcgc
     5521 atcgtgggcc tcgcccattg ctgccgatag atcctcccga gtcaccagct cgctctcggc
     5581 caggtcggag aaagtcgtcg cctgaaccac agcagctcgg gtcagcgcac cgttgccgag
     5641 agctgcgttc gtccaatgga ggtcggcggc gttccccgcc acgcgggcgg ccccgaccga
     5701 attggcgaag gtgagaccga ccacgaggac gacgaggacg agtgtcgctc cgatggcgat
     5761 accgaccgcg cgtcgccgcg ccgttggact ggtcaggcgc tccgtcccca tgatcggggt
     5821 tatcggagat ttcgcgcatt tttctttagg ggccggtgga cccttgacca gtcctcagag
     5881 ccttgctact tggtctaggc cgtcggcttg agacttaacg gtctgttgat tgggccacag
     5941 cgacagtggc cctcgtgaca actcatgaac ccactccttg caacacggaa tcgacgcgga
     6001 aatgaaacgg agggaactgc tccatctcct gtacgacagt ggctggatac tcgctcgtga
     6061 cgacgggggc tacctctggc gatgcccgtg cggtggcagg cacgacctcg gatccatccg
     6121 ctctcctgcc gatcagaagt tggccctccg gatgatcgat ctctgtcggc agacgatggc
     6181 cgaggaagtc aagcggtaga gccctccatc ctccacccac gctcgtcgag aatgcttcga
     6241 cgaggggatg gattctgtcg gggacggcag gtagtgtcga acgtatgttc gatctgggag
     6301 agccgaactg accggaggca tcgagac
//
LOCUS       DASTXO010000235         2724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_861762, whole genome shotgun sequence.
ACCESSION   DASTXO010000235 DASTXO010000000
VERSION     DASTXO010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861762"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..1392
                     /locus_tag="VFU96_04950"
     CDS             181..1392
                     /locus_tag="VFU96_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004430660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5112643.1"
                     /translation="MKRYLIIGVVLAMLVVACGGDTDTTTTAGATDTTEGATDTTEAP
                     TETTGGATETTAAAAGGMEELIAAAQEEGTLTTIALPHDWCGYGAVIDGFKAAYGLEV
                     NELDPGAGSAEELEAIDANRENPGPQAPDVIDVGLAFGPQAVEQGLIQPYQVSTWDEI
                     PDDAKDPDGYWYGDYYGVLSLEVNTAEVSAPPAEYADLLDPAYDGQVALSGDPLTSNA
                     AILSVWAAGLATGATGEEAAQAGLDFFAELNSAGNFVPIDGDSRTVGIGETPILIDWS
                     YNSIANDVNLAGNPEIETVVPEAGRIAGVYVQAISAFAPHPNAAKLWMEYLYSDEGQL
                     GWASAYCHPIRFEAMSAAGTIPPEVAEQLPSTEGAFFPSIEEIEAASAVIVEGWPSVV
                     GVTVEPSPSGG"
     gene            1415..2281
                     /locus_tag="VFU96_04955"
     CDS             1415..2281
                     /locus_tag="VFU96_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006471425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5112644.1"
                     /translation="MGGARRPPLTRFGSWLGVLPFFLFSGMFLIGPAVILAYRSLEAP
                     DGSLTLDNYRALTDANVLAAYGMSIRLSLLTAFVGGVFGFLLAWAISLGGLGRSTRSG
                     LTTFSGVASNFAGVPLAAAFVFTLGRVGVITKLFEWMGVDLYDTSFSLYSFWGLAFVY
                     LFFQFPLMLLVMLPAIDGLKQDWREAAENLGATPGQYWRRVAFPVLLPSILGTMILLF
                     GNAFGAHATAYALTGGSIPLATLLIGQQISGDVLNNPGLGYAVAMGMVFIMALCIVIY
                     SVLQRRSERWLG"
     gene            2269..>2724
                     /locus_tag="VFU96_04960"
     CDS             2269..>2724
                     /locus_tag="VFU96_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112645.1"
                     /translation="MAGLTISTTPLPVTTETEAPRGPARASRFWAWIIFTIAALYFLV
                     PLGATFWWSLRATEGVLGFEAYRRLFEDSNFWPSFTESVFNAIAAILLSLLIIVPTAY
                     WVTLKLPKLRPVVEFITLLPFVIPAVVLVFGLIRLYGRPPLLLTATYDSS"
BASE COUNT          435 a          885 c          859 g          545 t
ORIGIN      
        1 tcggctcctg actcggcacc gatcccgcgg tcgcccaaaa gagagcgagc cgggtgaccg
       61 agcattcatc cgttgttttc ctgacggtca tcgtggactc ctcggcgtgc cgcagaatct
      121 aggcgggcag tcgggcggcg agggtacgat ccggccgcgg tcattgaacc aaggagaatg
      181 atgaagagat atctgatcat cggtgtcgtc ctagcgatgc tggtagtcgc ctgtggcgga
      241 gacacggaca cgactaccac agctggggcc accgatacga ctgagggcgc gactgacacc
      301 accgaggctc ctaccgagac gactggcggc gccaccgaga ccacggcagc ggcagccggc
      361 gggatggaag agttgatcgc tgctgctcag gaggagggca ctctgacaac gatcgccctg
      421 cctcatgact ggtgtggcta tggggcagtg atcgacggct tcaaggcggc gtatggcctg
      481 gaagtcaacg agctcgatcc cggtgccgga tcggccgagg aactggaggc gatcgacgcc
      541 aaccgggaga accccgggcc acaggcgccc gatgtcatcg acgtcggcct tgccttcggg
      601 ccccaggcgg tggagcaagg gctgatccag ccctatcagg tctcgacatg ggacgagatc
      661 cctgacgatg ccaaggatcc ggatggctac tggtacggcg actactacgg ggtgctctct
      721 ctcgaggtca acaccgcgga agtatccgct ccgccggcgg agtatgccga tctcctcgac
      781 cccgcctacg acgggcaggt ggcgctgtcg ggtgacccgc tcacctcgaa cgcagccatt
      841 ctgtcggtgt gggccgctgg tctcgccacc ggcgccaccg gggaggaggc cgctcaggcc
      901 gggctggact tcttcgcgga gctcaactcc gcagggaact tcgtcccgat cgacggcgac
      961 tcgcgcaccg ttgggattgg ggagactccg atcctgatcg attggtcgta caactcgata
     1021 gccaatgacg tcaacctggc cggaaacccg gaaatcgaga cggtcgtgcc cgaggcagga
     1081 aggatcgccg gcgtctacgt ccaggcgata agtgcctttg cgcctcatcc gaacgccgcg
     1141 aagctctgga tggagtacct gtactccgac gaggggcaac tcgggtgggc atcggcttac
     1201 tgccatccca tcaggttcga ggcgatgtcg gccgcaggga ccatcccgcc tgaggtggcc
     1261 gagcagttgc cgagcaccga gggcgccttc ttcccctcga tcgaggagat cgaggccgca
     1321 agcgcggtca tcgtggaagg ctggccgagt gtggttggtg tgacagttga gccaagccca
     1381 agcggaggct gatcgggctc aagttacgaa ggcagtgggg ggcgcgaggc gccccccact
     1441 gacccgcttc gggagctggt tgggtgtgct cccgttcttt ctcttctccg ggatgttcct
     1501 gatcggcccc gcggtgatcc tcgcctaccg aagtctggaa gcacccgacg gcagcctgac
     1561 tctggacaat taccgggcgc tgaccgatgc caacgttctg gcggcctatg ggatgtcgat
     1621 ccggctcagc ctgctcacgg ccttcgtcgg tggtgtgttc ggcttcctcc ttgcctgggc
     1681 aatctccctg ggcggtctgg ggcgttcgac ccgttccggt ctcaccacct tctccggagt
     1741 cgcctccaac ttcgcaggcg tcccactggc tgccgccttc gtcttcactc tcggccgggt
     1801 gggtgtgatc accaagctct tcgaatggat gggcgtcgat ctctacgaca ccagcttcag
     1861 cctgtacagc ttctgggggc tggcattcgt ctacctcttt ttccagttcc ccctcatgct
     1921 cctggtcatg ctccctgcga tcgacggtct gaagcaggac tggcgagagg ccgctgagaa
     1981 cttgggcgcc accccgggtc agtactggcg ccgtgtggca ttcccggtcc tgttgccctc
     2041 gatcctgggg acgatgatcc tcctcttcgg caacgccttc ggtgcccacg ccacggccta
     2101 tgcgctcacc ggcggctcga ttccgctggc cacattgctg atcggtcagc agatatccgg
     2161 tgacgtactc aacaatccgg ggcttggcta cgcggtggcg atggggatgg tcttcatcat
     2221 ggcgctgtgc atcgtgatct actcagtcct ccagcgccgc tcggagaggt ggctgggttg
     2281 accatctcga cgaccccgct tccggtcacc accgagaccg aagctccgcg gggcccggcc
     2341 agggcaagcc gcttctgggc ctggatcatc ttcaccatcg cggcgctgta ctttctcgtg
     2401 ccgctcggag cgacgttttg gtggtcgctc cgtgcgaccg agggcgttct cggtttcgag
     2461 gcctatcgcc ggctcttcga agattccaat ttctggccct cgttcaccga gtcagtgttc
     2521 aacgccatcg cggccatcct cctcagcctg ctcatcatcg tccccactgc ctattgggtg
     2581 actctgaagc tccccaagct ccgcccggtg gtcgagttca tcacgttgct ccccttcgtt
     2641 atcccggctg tggtgctggt cttcggcctg atccgtctct atggccggcc ccccctgttg
     2701 ctcaccgcca cctacgacag ctcg
//
LOCUS       DASTXO010000236         4128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1304070, whole genome shotgun sequence.
ACCESSION   DASTXO010000236 DASTXO010000000
VERSION     DASTXO010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304070"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VFU96_04965"
     CDS             complement(<1..596)
                     /locus_tag="VFU96_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112646.1"
                     /translation="MAAPSVPEIETIDPGSVTTSDAARRTTSARWKATAYSLLGAASF
                     LLVWQLASQYTRTEFDLLPGPLEVAEQMGVYIVGDEAEGIPPGWVFVNFWETFRKTLY
                     GFAGALVIGIPIGILMGRYRYAKSFFFDFVYLAANVPLIVYAILGLLIFGIGDIGPAF
                     VVGLLVLPVITLNVAAGVEGVDPNLLAMSRSFGLPTQAA"
     gene            complement(619..1704)
                     /locus_tag="VFU96_04970"
     CDS             complement(619..1704)
                     /locus_tag="VFU96_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112647.1"
                     /translation="MVRMRNRYVVAGFAFLALTLTACAGGGGEEGADDSTPAEGEASM
                     RFVFSPDPVWNWLEDEGILAEMEEEAGMTIERNESEDEFAFFAGGHADIVSTGSYETP
                     VLESETDVETVTIGKYNMAKDIVVVAADSGYETFDDLEQGCSVGVESFSGSTIVWIAL
                     AQDLHGRTLGEGPDDLQMAITDFNTAPELVLSGDLCAGVSSIYHTLGYWLDESITGLY
                     DTQSASQIYGTEYVPGHEGMNSNNFVVTKSWYDEHPEEVAFFLSVWDRGLQEWASNRD
                     AILEAYPEDFGYTSDEELAFLKDWYENQFNEFVDTVYLDEEWIAGEAQVTDLLIAGGV
                     VPEGTTAPLHACIDPESGEETCLLPSS"
     gene            1880..2767
                     /gene="speB"
                     /locus_tag="VFU96_04975"
     CDS             1880..2767
                     /gene="speB"
                     /locus_tag="VFU96_04975"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEU5112648.1"
                     /translation="MGPATYARLPRIDEVPEWDVAIVGAPFDIGTSYRPGARFGPIGI
                     RHGARTIRNWHPDLEIHPFGTQQVVDAGDVPCSTYVIDDAIKTIEAAADELSQGSKRL
                     VTLGGDHTIAFPLLRSAHRRHGPVALVHFDAHVDTYDTYMNAAYTHGTPFRRAAEEGL
                     FVPGHSMHIGIRGSKYGPEDLGEDAGFGFTVLGTWEIEQIGIDGYVTRIREQVGELPL
                     YLSLDIDVLDPAFAPATGTPEVGGFSTRELMGILRGLRGLNLIGADVVEVAPAYDHAD
                     VTSIAAANVVYEMISLMSP"
     gene            complement(2778..3470)
                     /locus_tag="VFU96_04980"
     CDS             complement(2778..3470)
                     /locus_tag="VFU96_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112649.1"
                     /translation="MLRHTALFLFKDTTTDPEKLAALKGLAYLRYGCPSVEAVDFGTD
                     LFEGSSPLLEIKPWKRTPLWNARNEGPPSNFDMALHLDFEDLAGLEDYNKDDVHHEVA
                     VYNASVCRGEFTARIDWNYDGPSRIDRFGVRHTALFLWLDDAGESQKSDVGEAFRALS
                     SVPGVRSSLTADNVGTLTTDYDLIVDVTLDDVDAARGLLDHSAYKEATALAAAATKYE
                     WTARITHPMAAG"
     gene            3569..>4128
                     /locus_tag="VFU96_04985"
     CDS             3569..>4128
                     /locus_tag="VFU96_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112650.1"
                     /translation="MTNDDQVISVSDLYILPLDQFTAARDELAQQLKAAGDADEAKRV
                     AKLRKPSVAAWALNRASRNNPDEVGRLRESHRLLRQAGSRQAVEQASEMRRRAVASLT
                     DAAMAELDGGSLQTRDRVTRTLLAVATDAPGEADLAEGTLVRELEPSGGGWGDMELPP
                     VPEPDPQERAAAALEEARERARMLET"
BASE COUNT          747 a         1313 c         1327 g          741 t
ORIGIN      
        1 gcggcctgtg tcggaagccc gaaggagcga ctcatcgcca gcaggttcgg gtccacgccc
       61 tcgaccccgg ccgcaacgtt caaggtgatc acggggagca cgagcaggcc gacgacgaaa
      121 gccggaccga tgtcaccgat cccgaagatc agcagaccca ggatcgcata cacgatcaac
      181 gggacgttgg cggcgagata cacgaagtcg aagaagaagc tcttcgcgta ccgatagcgc
      241 cccatgagga tcccgatcgg gatcccgatg accaatgcgc cggcgaaccc gtagagcgtc
      301 ttccggaatg tctcccagaa gttgacgaag acccagcccg gcgggatgcc ctcggcctcg
      361 tccccgacga tgtaaacgcc catctgctcg gccacctcga gcggcccggg gaggaggtcg
      421 aattcggtcc gggtgtactg cgaggcgagc tgccacacga ggaggaacga tgctgcaccg
      481 agcagcgagt aggccgttgc cttccaccgg gcactggtcg tgcgccgggc ggcgtcagaa
      541 gtcgtgacgc tgcccggatc gatcgtttcg atttcgggaa cgcttggcgc tgccactctc
      601 gctccacccg tcttgctgtc aggaggatgg taggaggcag gtctcctcgc cggactccgg
      661 atcgatgcag gcgtgcagcg gtgcggtcgt cccttctggc accacgccac ctgcgatcag
      721 cagatcggtg acctgcgcct cgccggcgat ccactcctca tcgaggtaca ccgtgtccac
      781 gaactcattg aactggttct cgtaccagtc cttgaggaag gcgagctcct cgtcgctcgt
      841 atacccgaag tcctcggggt aggcctcgag gatggcgtct cgattgctgg cccactcctg
      901 caagccgcga tcccacacag agaggaagaa cgccacctcc tcggggtgct cgtcgtacca
      961 gctcttggtg accacgaagt tgttgctgtt catcccctca tgaccgggga cgtactcggt
     1021 gccgtagatc tgggaggccg actgtgtgtc atagagtcct gtgatcgact cgtcgagcca
     1081 gtagccgagg gtgtggtaga tcgagctcac accggcgcag aggtcaccgc tgagcacgag
     1141 ctcgggtgcg gtgttgaagt ccgtgatggc catctgcagg tcatccgggc cctcgccgag
     1201 ggtgcgccca tggagatcct gggccagagc gatccagacg atcgtcgacc cgctgaagga
     1261 ctcgactccg accgagcatc cttgttccag gtcatcgaag gtctcgtaac ccgagtcggc
     1321 agccacgaca acgatgtcct tggccatgtt gtacttgccg atggtgactg tctcgacgtc
     1381 ggtttccgac tcgagcacgg gtgtctcata actccccgtc gagacgatgt ctgcatggcc
     1441 accggcgaag aaggcgaact catcctcaga ctcgttccgc tcgatggtca tgccggcttc
     1501 ctcttccatc tcggcgagga tgccctcgtc ctcgagccag ttccagaccg ggtccgggga
     1561 gaacacgaat cgcatcgagg cttctccctc cgctggggtc gagtcgtcgg ctccctcctc
     1621 accgccgccg ccggcgcatg ctgtaagggt tagcgccagg aaggcgaaac cagccacgac
     1681 gtaccgattt ctcatgcgga ccaccggcct cccttgtcga gccgagctaa catgatggga
     1741 tgatagcctc cccctggttg gcgcgtcggg gcgttgccgg ctcgagtcat tcggcgggag
     1801 gccccatctg accggcaccc cattcaccga gcagggcggg catctgggcc caccggacgg
     1861 tcgtatctac ccccgcttca tgggcccggc cacctacgcc cggttacccc ggatcgatga
     1921 ggttcccgaa tgggacgtgg ccatcgtggg agcgccgttc gacatcggca ccagctatcg
     1981 gccaggtgcg agattcggcc cgatcgggat ccgccatggg gcgcgcacga ttcgcaattg
     2041 gcaccctgac ctcgagatcc atccgtttgg cacacagcag gtggtggacg ccggcgacgt
     2101 cccgtgcagc acctacgtga tcgacgatgc gatcaagacc atcgaggccg ccgcagacga
     2161 gctttcccag gggtcgaagc gattggtgac gctgggcggt gaccacacga tcgccttccc
     2221 gctacttcgc tcggctcatc gccggcacgg gcccgtcgcc ctggtccatt tcgatgcgca
     2281 tgtcgatacc tatgacacct acatgaacgc cgcctacacc cacggcacgc cgtttcggag
     2341 ggcggctgaa gaggggttgt tcgtccccgg ccactcgatg cacatcggca tccgggggtc
     2401 caaatacggg cccgaggacc tcggcgagga cgccgggttc gggttcaccg ttctgggcac
     2461 atgggagatc gagcagattg ggatcgacgg ctacgtcaca cggatccggg aacaggtcgg
     2521 ggaactgccc ctgtacctgt ccctggatat cgacgtcctc gatccggcat tcgctccggc
     2581 gaccggcacc ccggaggtcg gtggcttctc cacccgggag ttgatgggga tcctgcgcgg
     2641 gctccgtggg ctcaatctca tcggcgccga cgtggtcgaa gtggcccccg cgtacgacca
     2701 tgccgacgtg acatcgattg cggctgcaaa cgtcgtctac gagatgatct ctttgatgag
     2761 cccctgagag ctcacactca gccagccgcc atcggatgag tgatgcgggc cgtccactca
     2821 tatttggtgg cggccgcagc cagggctgtc gcctccttgt aggccgagtg atcgagcagg
     2881 cccctggccg catcgacatc gtcgagcgtc acatcgacga tcaggtcgta gtcggttgtg
     2941 agagtcccga cgttgtcggc ggtgagactc gatcggacgc cgggaaccga tgacagggct
     3001 ctgaacgcct caccgacgtc gcttttctga gattcgccgg cgtcgtccaa ccaaaggaag
     3061 agtgcggtgt gacgtacccc gaagcggtcg attcgcgagg gaccgtcgta gttccagtcg
     3121 atccgggcgg tgaactcccc ccggcacacc gaagcgttgt agaccgcgac ctcgtgatgg
     3181 acgtcgtcct tgttgtagtc ctcgagaccc gccaagtcct cgaaatcgag atgcagagcc
     3241 atgtcgaaat tcgaaggcgg gccttcgttg cgggcgttcc acagcggcgt ccgtttccag
     3301 ggcttgatct cgagaagtgg gctgcttcct tcgaagaggt ctgtgccgaa atcaaccgcc
     3361 tccaccgacg ggcatccgta gcgcagatag gcaagaccct tgagcgccgc cagcttctcc
     3421 ggatcggtgg tcgtgtcctt gaacaggaac agtgcggtgt gtctgagcaa gcggaactcc
     3481 tcgaagtgac ggtggaacct aacgtcctag gagtataggt atcggctgct ggtccctgct
     3541 gtcggtgcgg gtgcgatcat ggttggacat gaccaacgac gaccaagtca tctcagtctc
     3601 tgacctctac attctcccgc tcgaccagtt caccgccgct cgagatgagt tggcgcagca
     3661 actcaaagca gctggtgatg ccgatgaggc aaaacgagtt gccaagctgc gcaagccgtc
     3721 ggtggcggct tgggccctga atcgggcctc tcgaaacaac ccagacgagg taggccggct
     3781 tcgagaaagc catcggctgc tccggcaggc ggggtctcgg caggccgtcg agcaggcatc
     3841 ggagatgcgc aggcgggcgg tcgcgtcact cacggatgcc gcgatggcgg agctggatgg
     3901 aggctctctc cagacgcggg accgcgtgac ccggaccttg ctggcagtcg caaccgacgc
     3961 gccgggcgag gcagacctcg ccgaggggac cttggttcgt gagctggagc cgtcgggagg
     4021 tgggtggggc gacatggaac tccccccggt gccggaacca gacccccaag agagggcggc
     4081 ggcggccctg gaggaggccc gtgagcgggc ccgcatgctg gagaccca
//
LOCUS       DASTXO010000237         3676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_383127, whole genome shotgun sequence.
ACCESSION   DASTXO010000237 DASTXO010000000
VERSION     DASTXO010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383127"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VFU96_04990"
     CDS             complement(<1..263)
                     /locus_tag="VFU96_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene cyclase"
                     /protein_id="HEU5112651.1"
                     /translation="MVDEVPTRTRTIDWLLDGDPAIRWQTMRDLLGARDQVWRSEQER
                     VGSEGWGAKLLEHQDNDGRWTPRLYGKKWISTTYTMVLLRRIGL"
     gene            340..1254
                     /locus_tag="VFU96_04995"
     CDS             340..1254
                     /locus_tag="VFU96_04995"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEU5112652.1"
                     /translation="MEPATKIWVDGEFVPWDDATVHFLSHSLQYGTGVFEGIRAYKTP
                     EGTAVFRLHDHMVRLVASARAYELPMKESAEEMDEVCLELLRVNELESAYIRPFVFYG
                     SGAFGINPKSSPVHNYMAAFYLGSYLGEDGVEKGITAMVSSWRRISHTSFIPTAKGSG
                     QYLNSVLAKQEALRAGYDEALMLNDAGLVSEGSGMNLFTVSKDVVYTPPVSSGILEGI
                     TRNTVIELLTGEGVEVVQTDLARGSLYTADEVFLTGTAAEVTPVREIDGRVVGSGEPG
                     PITRKAQDLFSDLVAAKIPQYRHWLSFI"
     gene            1254..1754
                     /locus_tag="VFU96_05000"
     CDS             1254..1754
                     /locus_tag="VFU96_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112653.1"
                     /translation="MGQQPNIEINDADRPRTTLQPPPARRWRPSIKPGIPTSPEEVPS
                     GGRFGQTTPDGGWGWRLVNAAELPDDDPDLKAVLAALTIARAGALGRAPVGEDLEVAL
                     ALCGYGYDAPEAVVEGRERWKAAVPHEVRPGQTAVSEADRDLLVRKPGEIRELLAGSG
                     ASRDQH"
     gene            1767..2531
                     /locus_tag="VFU96_05005"
     CDS             1767..2531
                     /locus_tag="VFU96_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU5112654.1"
                     /translation="MKIVRIKAGDDIAYGVADVEGVLVYIGSPFVAWEPTETVVPWDA
                     AHLLAPVLPTKVVAVGRNYEDHAAEHDAVVPEEPLIFLKPATSVVGPDANVVHPAISE
                     NVHHEAELAAVISRVARNVKAEDASAYILGYTAANDVSARDLQRKDGQWARAKGFDTF
                     CPLGPAIETELDPLEKLAVICRVNGEVRQAGFTSDMVFGVAELVEFVTAVMTLLPGDV
                     ILTGTPAGVSKVEPGDLMEVEIDGIGVLQNRVVSGR"
     gene            2528..3304
                     /gene="gltX"
                     /locus_tag="VFU96_05010"
     CDS             2528..3304
                     /gene="gltX"
                     /locus_tag="VFU96_05010"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU5112655.1"
                     /translation="MKVRLRVAPSPTGHLHVGTARAALFNWLYARHHEGTFILRIDDT
                     DRERSTEIFEEDILDSLRWLGLDWNEGVDVGGPHGDYRQSSRYQRYQQAAAELVASGA
                     AYYDNRSPDELESLRRRAEEEHLHPGHYIRRPDKVITEGAIRLAVPQDQPVVFDDLVR
                     GEITFAARDIDDFVILRSDGTPTYHLASTVDDVDYEITHVARGEDLLPSTPKHILLTR
                     ALGAGEPLYAHLPLLFGTDGKKLSKRHGAISLSNYRDEGY"
     gene            3428..>3676
                     /locus_tag="VFU96_05015"
     CDS             3428..>3676
                     /locus_tag="VFU96_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112656.1"
                     /translation="MFDPDKLAWMNGEYIRAMDPSEFRSAARPHVESAVGRPLDEMEW
                     ARFDAVADLVQERTRLLPEAGGQVVFLFDDFEEYDRGAW"
BASE COUNT          666 a         1177 c         1171 g          662 t
ORIGIN      
        1 aggccgatgc gcctgagcag gaccatggtg taggtggtcg agatccactt cttgccatag
       61 agcctgggtg tccaccgacc gtcgttgtcc tgatgctcga ggagcttcgc cccccaccct
      121 tcgctaccca cccgctcctg ctccgaccgc cagacctggt ctctggctcc gagcagatcg
      181 cgcatcgtct gccatctgat ggcggggtct ccgtcgagga gccaatcgat ggtacgggtg
      241 cgggtcggga cttcatcgac catcgcccga ttcaaccatt accggccgaa aacgagaatc
      301 agtggtttcc ctgcgacgaa caccagttag ccttcggtga tggaaccggc aaccaagata
      361 tgggtcgacg gtgagttcgt cccctgggac gacgccaccg tccacttcct gtcacattcc
      421 ctccagtacg gaaccggtgt cttcgaaggc attcgcgcct acaagacacc cgagggaacc
      481 gccgtattcc gtctccacga tcacatggtg cggctggttg cttcggcccg agcctatgag
      541 cttccgatga aggagagcgc cgaagagatg gacgaggtct gtctcgagct gctgcgggtg
      601 aacgagctgg aatcggccta catacgcccc ttcgtcttct atgggtcggg cgccttcggc
      661 atcaacccga agagcagccc cgttcacaac tacatggccg ccttctacct cggcagctat
      721 ttgggcgaag atggtgtgga aaagggcatc accgcgatgg tctccagctg gcgtcggata
      781 tcccacacca gcttcatccc cacggccaag gggtcaggtc agtacctcaa ctcggttttg
      841 gcgaagcagg aggctctgcg cgccggttac gacgaggcgt tgatgctcaa cgacgcgggc
      901 ctggtgtccg aaggaagcgg tatgaacctc ttcaccgtca gcaaagacgt ggtctacacc
      961 ccacctgtgt catccgggat cctggagggg atcacccgca acacggtgat cgaattgctg
     1021 accggtgagg gtgtcgaagt ggtccaaacc gacctcgccc gggggagcct gtacaccgcg
     1081 gacgaggtct ttctcacggg taccgcggcc gaagtgaccc cggttcgtga gatcgacgga
     1141 agggtcgtcg gttccggcga gccgggcccg atcacccgca aggcacagga cctgttctcc
     1201 gacttggtcg cggccaagat ccctcaatac cgccactggc tcagcttcat ctgatgggcc
     1261 aacagcccaa tatcgaaatc aacgacgcgg accgcccgcg cacgaccctg cagccgccgc
     1321 cggcgcggcg ctggcgaccc agcatcaagc ccgggatccc gacctctccc gaggaggttc
     1381 ccagcggcgg ccgttttggc cagacgacgc ctgacggggg ttgggggtgg cggttggtga
     1441 acgctgcgga gctccccgac gacgaccccg atctcaaggc ggttctcgcc gcgttgacca
     1501 tcgctcgtgc cggcgcgttg ggccgggccc cggtgggtga ggatctcgag gtggccttgg
     1561 cgctttgtgg ctacgggtat gacgcccccg aagcggtggt cgagggccgg gagagatgga
     1621 aggcggcagt gccccatgag gtgagacccg gtcagacggc ggtgagcgaa gccgaccgtg
     1681 acttgctggt ccgcaaaccc ggtgagatcc gtgagttgct ggccggttcg ggggcatccc
     1741 gggaccagca ctagctttcc cccctcatga agatcgtccg catcaaggcc ggagacgaca
     1801 tcgcctatgg cgtggccgat gtcgagggcg tcctggtgta catcggctcc ccgttcgtcg
     1861 cctgggagcc gacggagaca gtcgtcccct gggacgccgc ccacctcttg gcgccggtcc
     1921 tccccacgaa ggtggtggcc gtggggcgca attacgaaga tcacgcggcg gagcatgacg
     1981 cggttgtccc cgaggagccg ctcatcttcc tcaagccggc gacatcggtt gtcggaccag
     2041 acgcaaatgt ggttcacccc gccatctcgg agaacgtcca ccatgaggcc gagctcgcag
     2101 cagtgatcag tcgcgttgct cggaacgtca aggccgagga tgcgtccgcc tacatcctcg
     2161 gttacaccgc ggccaacgat gtgagcgccc gcgacctgca gagaaaagac gggcagtggg
     2221 cccgcgccaa gggtttcgac accttctgcc ctctgggacc ggcaatcgaa accgagctgg
     2281 accccttgga gaaactggcg gtgatctgtc gtgtgaacgg agaggtccgc caggccggct
     2341 tcacctcgga catggtgttc ggcgtcgccg agttggtcga gttcgtgacg gcagtgatga
     2401 cgctgctacc aggtgatgtg atcctgaccg ggacaccggc cggtgtgagc aaggtggaac
     2461 caggcgacct gatggaagtc gagatcgacg gtatcggagt cctccagaat cgggttgtct
     2521 cggggcgatg aaggttcgcc tgagggttgc cccctcgccc accgggcacc tccatgtcgg
     2581 gaccgcccgt gccgccctct tcaactggct ctatgcgcga caccacgagg ggacgttcat
     2641 cctcaggatc gacgacaccg atcgggagag atcgaccgag atcttcgagg aggacatcct
     2701 cgactctctg cgctggctgg ggctcgattg gaatgagggg gtcgatgtgg gcgggcccca
     2761 cggcgactac cggcaatcca gccggtatca gcgataccag caggcggctg cggagctggt
     2821 cgcatcggga gcggcctatt acgacaaccg gtcacccgat gagttggaat cgctccgccg
     2881 ccgggccgag gaggagcatc tccatcccgg tcattacatc aggcggcccg acaaagtgat
     2941 caccgaaggt gcgatcaggc tggcggtgcc ccaggaccaa ccggtggtct tcgacgacct
     3001 ggtgcgaggc gagatcacct tcgctgcccg cgacatcgac gacttcgtga ttcttcgcag
     3061 cgacggcacc cccacctatc accttgcgtc gacggtcgat gatgtcgact acgagatcac
     3121 tcatgtcgcc cgcggggagg acctgctccc ctcgactccg aagcacatcc tgctcaccag
     3181 ggcattgggg gcgggcgagc ccttatacgc ccatctcccg ctgctgttcg gcaccgacgg
     3241 aaagaagctg tccaagcgtc atggcgccat ctccctgtcc aactatcggg atgagggcta
     3301 ttgacctgat gccgtcttca attacctctc actcctcggc tggtcgatcg atggcgaccg
     3361 caccatcttc tcgagggacg aggcgatcgc cgacttcgac ctggtcgacg tctctcgcaa
     3421 cccagccgtg ttcgaccccg acaagctggc atggatgaac ggcgagtaca tccgggccat
     3481 ggatccctcg gagtttcggt ccgcggcccg gccccatgtc gaatcagcgg tcggacgccc
     3541 actcgacgag atggaatggg ctcgcttcga tgcggtcgcc gatctggtcc aggagcgaac
     3601 ccggctcctt cccgaagcgg gagggcaggt ggtgttcctc ttcgacgact tcgaggaata
     3661 cgaccggggc gcctgg
//
LOCUS       DASTXO010000238         2265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1230683, whole genome shotgun sequence.
ACCESSION   DASTXO010000238 DASTXO010000000
VERSION     DASTXO010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230683"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..769)
                     /locus_tag="VFU96_05020"
     CDS             complement(38..769)
                     /locus_tag="VFU96_05020"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5112657.1"
                     /translation="MNQLLRVIFGVLVLMLAFVGCSSDSTPPAEVATTSALPPEPVVT
                     HSSSESEPGQRIEHGELILDVDDAKKQLHAQHHPHGVLETRRVPGAFQPPAMTDIKDF
                     ALAKSDLDVINPGPGEYVHVMEGQRHGYKNLTIGITYTAPGGAPPMHTHMGEESHVLL
                     EGQKVLYALGDKLFVMEGPYIVNIPPMVPHSFQNLDDKVAELVVIFPTNVWEYDVLDY
                     FPFSTPEAKAMAEEAKRAGAQTRGQ"
     gene            complement(815..1576)
                     /locus_tag="VFU96_05025"
     CDS             complement(815..1576)
                     /locus_tag="VFU96_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112658.1"
                     /translation="MVVLTATDYDFQTPDTLAAGWTTFRLVNNGGQPHMAQLVRLDPG
                     MTVEDFLAAYGEAFQTQGRRPEWARRLGGPTVTVPQQTNNATLYLEPGDYVWICLYNI
                     PDGIPHVVSHGMAEPFVVMQTEPTPSSEAAPKADVVMQLMDYAFGLDTPLTAGRHMIR
                     VQNAGSEPHEVSVLKLADGKTVEDVQAWMQNPGQAPLEDIVSVAGGVTSFVPGAEIYF
                     EVDLTPGEYVLLCVVTAPDGRSHMEHGMIQQIRVD"
     gene            complement(1673..>2265)
                     /locus_tag="VFU96_05030"
     CDS             complement(1673..>2265)
                     /locus_tag="VFU96_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5112659.1"
                     /translation="VSENVDVKKKVWSEIATRVDADAILASNTSSISISELGAVVPSP
                     HRFIGMHFMNPVPVMKLVEVIRGLETDDATTQTTLDLCRWLGKTPVEVHDYPGFVSNR
                     ILMPMINEAVFCVMEGVATKEAIDEVMKLGMAHPMGPLALADLIGLDVCLAIMEVLHR
                     DLGDSKYRPCPLLRKMVAGGRLGRKAGRGFYEYEKG"
BASE COUNT          408 a          729 c          681 g          447 t
ORIGIN      
        1 caggttgccg gcagatgccg acgggacgca gaccccctca ctggcctcgg gtttgcgccc
       61 cggctcgttt cgcttcctcc gccatggcct tggcttcggg agtcgaaaac gggaagtaat
      121 ccagcacgtc gtactcccac acgttggtcg ggaatatgac gaccagctcg gcgaccttgt
      181 catccaagtt ctgaaacgag tgcgggacca tgggcggaat gttgacgatg tagggcccct
      241 ccatcacgaa caacttgtcg cccagtgcgt agaggacttt ctgcccctcc aagaggacat
      301 gggattcttc gcccatatgc gtgtgcatgg gcggggcgcc gccgggcgcc gtatatgtga
      361 tcccgatagt caggttctta tagccatgcc gctgtccttc catgacgtgg acatactcgc
      421 ccggcccggg attgatcacg tcgaggtcgc tcttcgccaa tgcaaagtcc ttgatgtccg
      481 tcatcgccgg cggctggaag gcacccggta ccctgcgtgt ttccaggacc ccatgagggt
      541 gatgctgcgc gtgcagctgc ttcttcgcgt cgtcgacgtc gagaatcaat tcaccgtgtt
      601 cgatcctctg ccccggctcc gattcggagg atgagtgggt cacgaccggt tccggcggca
      661 atgcggacgt cgtcgccacc tccgccggcg gggtcgaatc cgacgagcac cccacaaacg
      721 ccaacatcaa gaccaacaca ccgaaaatta cgcgcagcag ttgattcact ccaatcctcc
      781 tctatttttc gaaacgaagg gccacgatcg gccgtcaatc gacgcggatt tgctggatca
      841 tgccgtgctc catgtgcgac cgcccgtccg gcgccgtgac gacgcaaagg agcacatatt
      901 cgccgggggt gagatccacc tcgaagtaga tctcggcccc gggcacgaag ctcgtgaccc
      961 cgcccgcaac gctcacgatg tcttccagtg gcgcctgacc cgggttctgc atccaggctt
     1021 ggacatcctc gaccgtcttc ccgtctgcga gcttgagcac gctgacctca tgcggctccg
     1081 agcccgcatt ctggactcgg atcatgtgcc gcccggcggt gaggggggtg tccagcccga
     1141 aggcgtagtc catcagttgc atgacgacat cggccttcgg tgccgcctcc gatgacgggg
     1201 ttggttccgt ctgcatcacg acaaacggct ccgccatgcc atgactcacc acgtgtggga
     1261 tgccgtcggg aatgttgtac agacagatcc acacatagtc accgggttcc agatacagcg
     1321 tcgcgttgtt cgtctgttgc ggaaccgtca cggtgggacc gccgaggcgc cgcgcccatt
     1381 ccggtcgccg gccttgggtt tgaaacgcct caccgtaggc tgcgaggaaa tcctcgacgg
     1441 tcatgcccgg atccagacgg acgagctgcg ccatgtgcgg ctgaccgccg ttgttcacca
     1501 ggcggaacgt ggtccatccc gccgctagtg tgtcgggcgt ctggaagtcg tagtcggttg
     1561 cggtaaggac gaccacgttg ggttgctggc cctgcttcga ctggcagcca gcgaggaggt
     1621 ttgacgagag taaaagcgcg agagcaaggc ggagaaccat cgtgacgccc gatcaccctt
     1681 tctcgtactc gtagaacccc cgccccgcct tccgccccag tcgtccgccc gcgaccatct
     1741 ttcgcaacag cgggcacggg cgatacttcg aatcgcctag gtcgcggtgc agaacttcca
     1801 tgatggcgag gcagacgtcg aggccgatca agtcggcgag ggcgagcggt cccatcgggt
     1861 gtgccatgcc gagcttcatc acttcgtcga tcgcttcttt ggtggccaca ccttccatca
     1921 cacaaaacac cgcctcgttg atcatcggca tcaggatccg attcgacacg aagccggggt
     1981 aatcgtggac ttcgaccggg gtcttcccca gccagcggca caggtcgagg gtggtctggg
     2041 tggtggcgtc gtcggtctcg aggccgcgga tcacttcgac cagcttcatc accggcaccg
     2101 ggttcatgaa gtgcatgccg atgaagcggt gcggcgacgg aaccacggct cccaactcgc
     2161 tgatcgagat cgaggaggtg ttgctggcga gaatcgcatc ggcgtcgacg cgcgtcgcga
     2221 tctccgacca caccttcttc ttcacgtcca cgttctcgct caccg
//
LOCUS       DASTXO010000239         8450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1488421, whole genome shotgun sequence.
ACCESSION   DASTXO010000239 DASTXO010000000
VERSION     DASTXO010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8450
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488421"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VFU96_05035"
     CDS             <1..279
                     /locus_tag="VFU96_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112660.1"
                     /translation="DPLVVAALGALALIAVLREPLEPISFVDSRTKKFIRRDRLQAGR
                     VLSAVLVFFGAVVSGANAPAVLPMAVVVAAALASTEVERLQRASPAPG"
     gene            290..505
                     /locus_tag="VFU96_05040"
     CDS             290..505
                     /locus_tag="VFU96_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018518417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEU5112661.1"
                     /translation="MCRSIKVLREGATPAPTEEIEAAALQFVRKISGFSRPATHNEDV
                     FHQAVHEISEASMRLLDGLEIRGAKAS"
     gene            complement(502..1173)
                     /locus_tag="VFU96_05045"
     CDS             complement(502..1173)
                     /locus_tag="VFU96_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEU5112662.1"
                     /translation="MLIRNRRVAQRRRAMLIPTVIEQTSRGERAYDIYSRLLGHRVMF
                     LGRQVDADTANLVVAQLIHLESEDPEKDISLYINSPGGEMSSMMAIYDTMQHIRPEVM
                     TACIGMAASAAALILAAGAPGKRYVLPHARVLIHQPHLMGGGLEGQASDIEIHAREII
                     RQKEEMNAILARHTGQTVETITRDTDRDRWLTAEQAVDYGLVDAVMSPNRVPAGLDSL
                     PVDQT"
     gene            1203..1619
                     /locus_tag="VFU96_05050"
     CDS             1203..1619
                     /locus_tag="VFU96_05050"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEU5112663.1"
                     /translation="MQEEFFEEMSGDIKVERRVSLPVEPGTVWERLIDENFVGDWMGG
                     EVSIEARPGGAIILDPDEGAPVWGTVEDVVPGRRLQWSWRTDDGLPTQVEIELEPAEE
                     GTILTVRETLLPWTVTGLPPQWGGSPFPRASLSAAA"
     gene            1619..1924
                     /locus_tag="VFU96_05055"
     CDS             1619..1924
                     /locus_tag="VFU96_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU5112664.1"
                     /translation="MIPHEVFAALADPTRVEVLSRLANGGPATATEISAGMPISRQAV
                     TKHLAALDAAGLVARHPAGREVRYTFDPEPLGDLVRWVETVGDTWDQRLGRLSRSIE"
     gene            complement(1996..2847)
                     /locus_tag="VFU96_05060"
     CDS             complement(1996..2847)
                     /locus_tag="VFU96_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112665.1"
                     /translation="MKRFRVVILGVAAAVLLAATAAVAAPTVFKDVPADHNFAVAINW
                     MAENGTTKGCNPPANDMFCPEDYVTRGQMSAFMKRLVESDLMVAGAGVAGPQGIQGPK
                     GDKGATGARGPQGATGATGAQGVPGLPGADGADGADGADGIDGAAGIDGAAGIDGQDG
                     ASGLQGEQGEKGDPGIQGEQGEKGDKGDPGIQGEQGIPGTGATFTRVVGTPSASNDGQ
                     TKSATATCPAGVVVGGGVLFLGAQPHNLTVTGDYPSDTTTWTVSGAENVLHNGNWSIQ
                     AFAICAS"
     gene            complement(3005..3835)
                     /locus_tag="VFU96_05065"
     CDS             complement(3005..3835)
                     /locus_tag="VFU96_05065"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEU5112666.1"
                     /translation="MPDAIRAMEHAFSAEAETPMRTIVGSSLVMPGRLEDIIAVKVVS
                     TVPGDPAGLVVVFAPDGSPLGLVHGPTLTAIRTGAVCGLATALLATTGASTLAMLGAG
                     AMAFDQIEAVRTVRPIDRVLVWSRNPVNARLLAEMVGGEHVDDADEAAAQADVISCAT
                     PATSPIFSESSVREGAHVNAIGAYTPEMAELAPDLLERAYVVVDDLAAAAEEAGDLIQ
                     AGRLPNTSLRELLAGTSPEIGEDVTVFKSVGVAAQDVAAAHLALVNAERLGVGTLLLA
                     "
     gene            complement(3877..5115)
                     /locus_tag="VFU96_05070"
     CDS             complement(3877..5115)
                     /locus_tag="VFU96_05070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5112667.1"
                     /translation="MNRTADVVVVGAGIIGSSIAFQLARHGAGRVVALDKGKGPAEGS
                     TGASSSICRCRYTHPEVVRLAFHGQEAYGNWAEFTGLDNPRSGLQRVGVLWMMGESKD
                     RVGSDADKLFGEGVKAEAIGPDDVTELFPALSTCGVPFDLTGEIEHECAPGEAFLYET
                     LGGYAEPVGANQDLIEATRRMGGSVEFNSRVAGVLKENGRVSGIRLEDGTEISADLVV
                     NAAGPWCNQLNAMAGAELRWTLTPTRIQTVYRSWPPDLGPIPVGADASTGIYFRPETG
                     GQQVLVGSVLAEDEEEVIEDPDDYKRVPDAGFTELKLAAFHHRIPGLEARGGVSGVAG
                     LYTINREDVHPVVGPSGVDGLWVANGFSGHGFKLAPSIGSLIAQAYTGASIEFDTDVP
                     MSFLAVDREPIDVAVKNVLA"
     gene            complement(5112..6119)
                     /locus_tag="VFU96_05075"
     CDS             complement(5112..6119)
                     /locus_tag="VFU96_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEU5112668.1"
                     /translation="MEEERIALFVDFENLAIGVREALGGMEFDFEPIGNALAERGRVV
                     TRKAYADWGMFSDARQKLTRSHIELIEIPQRMGASRKNAADIKMAVDAIEVAFERDYI
                     TTFVICTGDSDFTPLVHKLRELNKKVIGVGVKASTSALLPPASDEILFYENLDGVEPS
                     RATAPAEESEQVDDSIPELESLITTTLSGLLRSGGGEVLASSLKRALLRKDSTFSEAD
                     YGFRAFGELLNHLESQGMIETSEGPGRGDPIVQFPSAGSGEGAAFDLLVEVVTDMAKK
                     GTPVFLSGLKNQLRKKDQTFSEKKFGYSGFLQFCKAAATRGQIELTWDEEAGDYRVAV
                     A"
     gene            6266..>8450
                     /locus_tag="VFU96_05080"
     CDS             6266..>8450
                     /locus_tag="VFU96_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112669.1"
                     /translation="MLYTDPVLRDTLPTGQPRRYTEVLGVGPADDEFNTQAFHWGGLT
                     AGSRLTAFWILLAPFAFANVAGWMSTNRKNRFGHAMIRVTGLGLTALFLAQVFTAVVL
                     LPFIWLARQDDLEVWGLTIVVTDSMLRWGLLILVVALTGLFLLLVLKASTQSHFEPSD
                     TDKQSQLLAWPTVEALDPRLSDSERKAIEQDLIPLDDEPRQWEDPVDAVITDPRMWRP
                     NSILDRLRRLHLAIGYGIVALEVAIWTETAWAVSVVAVFLVAIVLVTILTSFAPTNRW
                     VLRATAVAPSVGVLVWVSTLVTIFTGSVGDEVITPHVVSFVTALVMGIFALLSVSAGL
                     ISVGALVFATFFGAVLGLAIGIVIESILGLNPPDTPGILEQNGAAWVAIAMLGFLWWL
                     AVTAIFLSWRKTEHTAEKGVLALLRRVVLQAPVLFKSAAVYGIVAGGIAAFLGWQYEA
                     WEPRALVVPAPDSPVYDIAIFAGAFLVIFLTLRVAHHFGWKLAPLVILGAGLIVLAAY
                     RDFFIVEFFKVQIALKGNLVDLAVAIAIIVPGAFMLRSIWTGAGSSEEGRKKRRNVGI
                     LWDMGSFWPRWYHPLAPPAYGPVAVTQLRKELESHQRDVLGAHSQGSLISAVALIYTD
                     GAKKPHRFITYGSQLGVLYPGMFPGAGIGDEDGLVEKVYAAYGSEWLNLYRDTDPIGG
                     HFVQRLDTQNLAVHSSTGHSRYEPTDEYKEARRGVSGVSSSTISPV"
BASE COUNT         1426 a         2792 c         2646 g         1586 t
ORIGIN      
        1 gacccgctcg tggtggcggc cctgggcgcg ctcgctctca ttgcggtgct ccgggagccg
       61 ctcgagccaa tctctttcgt cgacagccgg accaagaagt tcatcaggcg agaccggctt
      121 caggcgggtc gggtcctttc cgccgtcctc gtgttctttg gcgcggtggt gtctggagcg
      181 aacgcgcccg ccgtcttgcc aatggccgtt gtcgtcgccg ccgcccttgc ctcgaccgaa
      241 gtggagcgtc tgcaacgggc aagccccgcc cccgggtagc gtgggccgca tgtgtagatc
      301 gatcaaagtc ctcagggagg gcgccacacc tgctcccacg gaggagatcg aggcggcggc
      361 gcttcagttc gtccgcaaga tctccgggtt cagccgcccg gccacccaca acgaggacgt
      421 cttccaccag gccgtccacg agatcagcga agcgtcgatg cgtctcctcg acgggctcga
      481 aatccggggt gcgaaggcct cttaggtctg gtccaccgga aggctgtcca gaccggccgg
      541 cacccggttc ggcgacatca ccgcgtccac gaggccgtaa tcgaccgcct gctcggcggt
      601 cagccaccgg tcacgatcgg tgtcacgggt gatcgtctcc acggtttgtc ccgtgtggcg
      661 ggcgaggatg gcgttcatct cctccttctg gcgaatgatc tcccgggcgt ggatctcgat
      721 gtccgacgcc tggccctcga gcccgccccc catcaggtgg ggctggtgta tgagcaccct
      781 ggcatggggc agcacatatc gcttgcccgg ggcacccgcc gcgaggatca gcgccgccgc
      841 cgacgcagcc atgccgatgc aggctgtcat cacctcgggc ctgatgtgct gcatggtgtc
      901 gtagatcgcc atcatcgagg acatctcacc tccgggtgag ttgatgtaga gcgagatgtc
      961 cttctctgga tcttcgctct cgagatggat caactgggcg acgaccaaat ttgccgtgtc
     1021 ggcgtcgacc tgacggccga gaaacatgac tcggtgcccg agcaatcggg agtagatgtc
     1081 gtacgcccgc tctcccctgc tcgtctgctc gatgaccgtt ggtatcagca tcgctcttct
     1141 cctttgtgca accctccggt tgcgaatcag catacacgac gcgcaactgc taagttgcag
     1201 gtatgcagga agagttcttc gaagagatga gcggcgacat caaggtcgag cggagggtga
     1261 gcttgcccgt cgaaccgggg actgtctggg agcgtctcat cgacgaaaac ttcgttgggg
     1321 actggatggg cggggaggtc tcgatcgagg cccggcccgg cggcgccatc atcctggacc
     1381 ccgatgaggg ggctccggta tggggcaccg ttgaggacgt cgtccccggg cgtcgtctcc
     1441 aatggagctg gcggaccgac gacgggctcc ccacccaagt ggagatcgaa ctggagccgg
     1501 cggaggaggg caccattctc accgtccggg agaccctgct gccctggact gtcaccggtc
     1561 ttcctccgca gtggggtggt tcccctttcc cgagggcttc tctgtccgca gcagcctgat
     1621 gatcccccac gaggtcttcg ccgcattggc cgatccgact cgtgtcgagg tgttgagccg
     1681 actcgccaac gggggcccgg caaccgcgac tgagatctcc gccggtatgc cgatctcccg
     1741 acaggcggtg accaagcatc tggccgccct cgacgccgcg ggtctcgtgg cgcgacaccc
     1801 cgccgggaga gaggtccgct acaccttcga tcccgagcct ctgggcgatc tcgtccggtg
     1861 ggtcgaaacg gtcggcgaca cctgggatca gcgtctcgga cggctcagcc gaagcatcga
     1921 gtagcgatgc cgaaatcgca cgcaagagcc cccgccacag cgggggctct tctcgccgag
     1981 tgccctaggc gaagctcagg aagcgcagat tgcgaaggcc tggatcgacc agttgccgtt
     2041 gtggaggacg ttttccgcac cagacacggt ccacgtggtc gtgtctgagg ggtagtcgcc
     2101 agtgacggtc aggttgtgcg gctgggctcc caggaagagg acaccgcccc cgacaaccac
     2161 acctgctgga caggttgcag tcgccgactt ggtctggccg tcgttcgatg cgctaggagt
     2221 gccaacgact ctggtgaagg tggcaccagt accggggatg ccttgctcgc cttgaatccc
     2281 aggatcaccc ttgtcaccct tttcaccctg ctcgccctgg atcccaggat cacccttttc
     2341 accctgctcg ccctggagac cggaagcgcc gtcctggccg tcgatgccag cggcaccgtc
     2401 aatgccagcg gcaccgtcaa tgccgtcggc cccgtcggcc ccgtcagccc cgtcggcacc
     2461 gggcagaccg ggcacaccct gggcaccggt agcaccggtt gcaccctgcg gaccccgggc
     2521 accggtggca cccttgtcac ccttgggacc ctgtattccc tgcgggccgg cgacaccggc
     2581 gccggcgacc atgagatccg actcgaccag ccgcttcatg aaagcggaca tctgaccgcg
     2641 ggtcacataa tcctccgggc agaacatgtc gttcgccgga gggttacacc cttttgtggt
     2701 cccgttctcc gccatccagt tgatggcaac cgcgaaattg tgatctgcgg ggacgtcctt
     2761 gaaaaccgtc ggcgcggcca cggcggcagt cgccgccaga agaaccgctg ctgccacccc
     2821 gaggatgacg actctgaacc tcttcatttc cctacttcct tgcttcttgg gtctgacctg
     2881 attcccagat gggcggctat cggcgctacg ccgcgaaact ttgagcacac gcgcacaaat
     2941 agggccgata ggactagtcc cgtgaatcgc tgggtttgga gccgaggtgg ctcttgagac
     3001 gtggctaggc caggagcagg gtgcccaccc cgagacgctc ggcgttgacg agggcgaggt
     3061 gggctgcggc gacatcctgt gcggccacgc caacactctt gaacaccgtc acgtcctccc
     3121 cgatctcagg gctcgtcccc gccaacagct cgcgcaacga agtattcggc aaccgcccgg
     3181 cctggatcag gtcgccggcc tcctccgccg ccgcggcgag gtcgtcgacc accacgtacg
     3241 ccctctccaa gaggtcgggg gcgagctctg ccatctccgg cgtgtaggcg ccgatcgcat
     3301 tgacgtgcgc tccttcgcgg acgctcgact cggaaaagat tggcgaggtg gccggtgtgg
     3361 cgcaggagat cacatcggcc tgggccgccg cttcgtcggc gtcgtcgaca tgctcgccac
     3421 cgaccatctc ggccagcagc ctggcattga ccggattccg ggaccagacc aggacccggt
     3481 cgatgggccg gaccgttcgg acggcctcaa tctgatcgaa cgccatcgcc ccggccccga
     3541 gcatggccag cgtgcttgcc ccggttgtcg ccagcaacgc cgtcgccaac ccacagaccg
     3601 cgccggtgcg aatcgcggtc aatgtcggcc cgtggaccag accgagaggg ctcccgtcgg
     3661 gtgcgaagac cacgaccagc cctgcgggat caccgggcac ggtcgacacc accttcaccg
     3721 cgatgatgtc ctcgagcctc cccggcatca ccagcgaact gccgacgatg gtccgcatcg
     3781 gggtctcggc ttcggcggag aaggcgtgct ccatcgcccg aatcgcatcg ggcatcggaa
     3841 gtgcttgtcg ggtctctgcg gagttgaggt aacgcatcag gccagaacgt tcttcacggc
     3901 cacatcgatc ggctcccgat cgaccgcgag aaagctcatg gggacatcgg tgtcgaactc
     3961 gatggaagcg ccggtgtatg cctgggcgat cagcgacccg atcgatggag cgagcttgaa
     4021 gccgtgaccg gagaacccat tcgccaccca aagcccatcg accccggacg ggccgaccac
     4081 tggatggacg tcctcccggt tgatggtgta gaggccggcg acgccagata cacctccccg
     4141 agcctccaag ccagggatgc ggtgatggaa ggcggcgagc ttcaactcgg tgaacccggc
     4201 gtcggggacc cgcttgtagt cgtcgggatc ctcgatcacc tcttcttcgt cctcggccag
     4261 caccgacccg accaggacct gttgcccccc tgtctccggc cggaagtaga tgcccgtcga
     4321 ggcgtctgcc ccgaccggga tcggtcccag atccgggggc caggaccgat agaccgtctg
     4381 gatccgggtc ggggtcaggg tccagcgcaa ctccgcgccg gccatcgcat tgagttgatt
     4441 gcaccacggg ccggcggcat tgaccaccag atccgcgctg atctcggtgc cgtcctcgag
     4501 acgaatcccc gacacccggc cgttctcctt caagacaccg gctacccggg agttgaactc
     4561 gacgctgcca cccattcgac gagtggcttc gatcaggtcc tgatttgccc caaccggctc
     4621 ggcgtagcca ccgagcgtct cgtagagaaa agcctcgcct ggagcacatt cgtgctcgat
     4681 ctccccggtg aggtcgaagg gcacaccgca cgtcgacagg gcggggaaga gctctgtgac
     4741 gtcgtcggga ccgatggcct cggcctttac cccttcgccg aagagcttgt cggcgtcaga
     4801 cccgacccgg tccttgctct cccccatcat ccagagcaca ccgacccgct gcagcccaga
     4861 ccgcgggttg tccaagccgg tgaactcggc ccagttgccg taggcctcct gaccgtggaa
     4921 ggcgagacgg accacctcag gatgggtgta acggcatcga caaatggacg atgaggcacc
     4981 cgtcgagccc tcggccgggc cctttccctt gtcgagggcc accaccctgc ccgcgccatg
     5041 acgggccaac tgaaacgcga tcgaggaccc gatgatgccg gcccccacca cgaccacatc
     5101 agccgtgcgg ttcatgcaac ggcgacccgg tagtccccgg cttcctcgtc ccaggtcagc
     5161 tcgatttggc ccctggtcgc agccgccttg cagaactgga ggaaccccga gtagccgaac
     5221 ttcttctccg aaaaggtctg atccttcttg cgaagctgat tcttgagacc ggacagaaac
     5281 acgggcgtgc ccttcttcgc catgtcggtc accacctcga ccaggaggtc gaacgccgca
     5341 ccttcgccag atccagcgga ggggaactgc acgatcggat cgccacgccc cgggccttct
     5401 gatgtctcga tcatgccctg ggactccaga tggttgagga gctcaccgaa ggccctgaag
     5461 ccgtagtcgg cttcgctgaa cgtcgagtct tttcggagca gcgcccgctt caaggaggag
     5521 gcgaggacct ctccccctcc gctcctgagc agaccggaaa gagtcgtcgt gatcaacgac
     5581 tccagctcag gaatggagtc gtcgacctgc tcgctctcct ccgccggagc ggtggcacga
     5641 ctcggctcga caccatccaa attctcgtag aagaggatct cgtcacttgc cggtgggagc
     5701 agtgccgatg tcgacgcctt gaccccaacc ccgatgactt tcttgttcaa ctcacgcagc
     5761 ttgtgcacga gcggggtgaa gtctgaatct cccgtgcaga tgacgaacgt cgtgatgtag
     5821 tcgcgttcga acgcgacctc gatcgcgtcg acggccatct tgatgtcggc ggcgttcttg
     5881 cgacttgcgc ccatgcgctg gggaatctcg atcagttcga tatgactccg cgtcagcttc
     5941 tggcgtgcat cagagaacat gccccagtct gcgtaggcct tccgggtcac gacccggccc
     6001 ctctcggcca gggcattgcc gatcggctcg aagtcgaatt ccatccctcc gagcgcctct
     6061 cgcacgccga tggcgaggtt ctcgaagtcg acgaacagtg cgatgcgctc ttcttccatg
     6121 gaggcaaggc taggcgagcc ctaggtgtcc atccccatgc agcacgggcc gcgccgcaag
     6181 tagcttcgtg ctgtcaggac gagggggatc tacgacgaag agcgagcttc gagtgcacgg
     6241 agtctcgggc acacagccgc gagacatgct ctacaccgat ccggtgctga gagacacgct
     6301 gccgaccggt cagcctcgtc gctacaccga ggtgctgggc gttggcccgg ccgatgacga
     6361 gttcaacacg caggcctttc actggggtgg gctcacggcg ggcagccgtc tcaccgcctt
     6421 ttggatattg ctcgccccgt tcgccttcgc caatgtcgcg ggttggatga gcacgaatcg
     6481 gaagaaccga ttcgggcacg cgatgatccg cgtcaccgga cttgggctca ccgcgttgtt
     6541 cctggctcag gtgttcacgg ccgttgtgtt gcttcccttc atctggctcg cgagacagga
     6601 tgatctcgaa gtatggggcc tgaccatcgt tgtgaccgac tcgatgctga gatggggcct
     6661 tctgatcctc gtcgtggccc tcaccggtct gtttctcctc ctcgtgctca aggcctccac
     6721 ccagtcacac ttcgagccga gcgacaccga caaacagtcg cagttgctgg cgtggccgac
     6781 ggtcgaggcc ctggatccgc gcctctcgga ctcggagcgg aaagcgatcg aacaggacct
     6841 gatcccgctc gacgacgagc cacgtcagtg ggaggatcct gttgatgcgg tgatcaccga
     6901 tccgaggatg tggcggccga actcgatcct tgaccgtctc cgccggcttc acctcgccat
     6961 cgggtacggg atagtcgcgc tcgaggtggc gatttggact gaaacggcgt gggccgtgtc
     7021 cgtcgtcgcc gtctttctcg tggcgattgt gctggtcacg atcctgacgt cgtttgcccc
     7081 gaccaatcgc tgggtcctga gagctactgc ggtggccccg tcggttgggg tcctcgtctg
     7141 ggtttcgacg ctggtgacga tattcacagg atcggtcggc gatgaagtca tcacgccgca
     7201 cgtggtgagc ttcgtcactg cactcgtcat gggaatcttc gcgttgctct cggtctcagc
     7261 cggcctcatc tcggttggtg ccctggtgtt cgccaccttc tttggtgccg tgctcgggct
     7321 ggcgatcggg atcgtcatcg agtccatcct cgggctcaac ccgccggaca ctccggggat
     7381 cctggaacag aacggtgccg cgtgggtcgc gatagcgatg ctcggcttcc tctggtggtt
     7441 ggcggtcact gcgatattcc tcagctggag gaagaccgag cacacggcgg aaaagggcgt
     7501 cctggcattg cttcggagag tggtgcttca ggctccagtc ctgttcaagt cggctgccgt
     7561 ctatgggatc gttgccggcg gcatcgccgc gttcctcggc tggcaatacg aggcgtggga
     7621 gccaagggcc ctggtagtgc cggcgccaga cagccctgtc tacgacattg ccatcttcgc
     7681 cggtgctttc ctggtcatct tcctgacgtt gcgtgtggcg caccacttcg gttggaagct
     7741 cgcacctctg gtgattctgg gcgccgggct catcgtcctc gccgcctacc gggatttctt
     7801 catcgtcgag ttcttcaagg tccagatcgc actcaaaggg aatctcgtcg atctcgcggt
     7861 ggcaatcgcc atcatcgtcc ccggcgcctt catgctccgc agcatctgga ccggggccgg
     7921 aagcagtgag gagggccgga agaagcgaag gaatgtgggg atcctctggg atatgggctc
     7981 gttctggcct cgttggtacc acccgctcgc cccaccggca tatgggccgg tcgccgtcac
     8041 gcaactccgg aaggaactgg aatcccatca gagagatgtt ctgggagcgc actcgcaggg
     8101 ctccttgatc agcgccgtcg ccctgatcta cacagacggc gccaagaagc cacatcggtt
     8161 catcacctac gggtcgcagc tcggggtcct gtacccgggg atgttccccg gggctggcat
     8221 cggtgacgag gacggattgg tcgagaaggt gtatgccgcc tacggctcgg agtggttgaa
     8281 cctctacagg gacacggatc cgatcggggg ccacttcgtc cagaggctcg acacgcagaa
     8341 cctggctgtc cactcatcta ccggtcactc tcggtacgag ccgacggatg agtacaagga
     8401 ggcgaggcga ggggtcagcg gcgtgtcctc ttctaccatt tccccggttg
//
LOCUS       DASTXO010000240         6374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1451978, whole genome shotgun sequence.
ACCESSION   DASTXO010000240 DASTXO010000000
VERSION     DASTXO010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451978"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..2492
                     /gene="gyrA"
                     /locus_tag="VFU96_05085"
     CDS             57..2492
                     /gene="gyrA"
                     /locus_tag="VFU96_05085"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU5112670.1"
                     /translation="MTQQNPPAEGVPQVEITDEMEQSFVDYAMSVIVSRALPDVRDGL
                     KPVQRRIIYSMFENNITSGSAHRKCSKVVGDVMGNYHPHGDTAIYDALVRMGQDFSMR
                     HLLIDPQGNFGSIDDPPGAARYTECRLTKLSNHLLEGIREDTVDFEDNYSGEMQQPTV
                     LPARFPNLLVNGSQGIAVGMATNIPPHNLNEIIDACTHVIDNPDATTEELLQFVKGPD
                     FPTGGFIVGTGGIRDALTTGRGSVKIRAVCDVQEIRKGRTAIIATELPYQVSIERIQA
                     KIRELVENKRLEGIADIRNESSDRVGIRLVIELKSGAVPQVVLNQLFKNTPLQDSFGY
                     NMVSLVDGVPRTLDLAEMVGYYLDHQMEVVERRTRFRLAEAQARAHILEGLIIAVDNI
                     DEVIKIIRGSADTADARANLIDRFELSEIQANAVLDMPLRRLTALEVDKLRSELAELH
                     DLITELEAILADPKRRWAIIRDELSEIKEKYGESRRSRVIPDEGDLSLEDLIADDELI
                     VTVSSAGYIKSVPASTYKTQGRGGRGVKAAEVQETDVIQHLVHTTAHAYLLFFTNKGL
                     VHRIKAHGIPRQSRTAKGVLAQSVLPLEPEERVQAIIDTRDYETGRYLVMVTKQGIAK
                     KTEFKEYESRNQTLVAIKLVDGDELVSVRTTNGENDVLIFTKEGMGIRFKEADLRSMG
                     RGTQGVRGIRLRDGDQVVAAVSNVDGDEVLLVTSAGYGKRTAIGEFRRQTRGGIGVKA
                     FKLTRVRGNLVGGRAVTKDDDVFLISSAGIGIRTPVATISRQKRDSTGVRVIHVGDGN
                     ELSALAIVSDEVE"
     gene            2571..3080
                     /locus_tag="VFU96_05090"
     CDS             2571..3080
                     /locus_tag="VFU96_05090"
                     /inference="COORDINATES: protein motif:HMM:NF023511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HEU5112671.1"
                     /translation="MATVRRVRRIIRKIDPWTALKVSAVVWAVLALALVLGGVIFWSV
                     LERAGLPQRLTDFMVEITLIDEGAQPFANVEQFFRVMVFGSVVWWVTMTGATVAGAIV
                     YNLVSDVVGGLEVVVLEETLNPVVAPAPSQVLHPPTDYRPGNGSVTPPPDHADIPTEE
                     SPRVLDPRL"
     gene            complement(3087..3446)
                     /locus_tag="VFU96_05095"
     CDS             complement(3087..3446)
                     /locus_tag="VFU96_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5112672.1"
                     /translation="MLTLGSVVWGVRDVPEAIRFWGEALDYEPLYEPEDDWAILVPRE
                     GSGQRISIRLVTSDPLPRQRHHLDLYASDQSAEVDRLIGLGARRVEWTYEEDADYVVL
                     ADPDGNQFCVIDASEEG"
     gene            complement(3456..4091)
                     /locus_tag="VFU96_05100"
     CDS             complement(3456..4091)
                     /locus_tag="VFU96_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112673.1"
                     /translation="MKVGVLASGDTAVRAAHSLSAHDSVEEVVVVGPARSRSFRVVPD
                     ASELDLLIGSGDAAPMRARKLGVPLLWDGDNAAEGVVVWGASPVGLALALGAREPDPR
                     IIAVAHPDLDEGFDHQVRFPDPVGTAKVADATFGGRHVALAQSPDRFAACLAVGSERQ
                     VTIVDDGAFMSGIALAAAVDAVGEEPAPVWDSALVYLQAAAAMGLVMAEDR"
     gene            complement(4265..5491)
                     /locus_tag="VFU96_05105"
     CDS             complement(4265..5491)
                     /locus_tag="VFU96_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HEU5112674.1"
                     /translation="MTTSRLDIDAIRARFPALSRREHGRPVAYLDGPGGTQVPKAVID
                     AMNAVLTEGVSNLGGDFGASQLAEDTTRLGREAMADFFNADPSEISFGQNMTSITFAV
                     SRALARAWEPGDAIVVTSLDHDANFTPWVRAAEDVGAEVRVAHFDVITGELDPAVVAD
                     LLDDRVRLVAVCVASNAIGTLVDVTALTSLAHEAGAMVYLDSVHAGPHHLVDVRSIDC
                     DFLVASAYKFFGPHTGILFGKLDRLAELEFYKVRPAPSDPPDKLETGTQSFESIAGVT
                     AAVDYLASLGDGTNRRARLVESYRGIQEHEHALSTRFIEGVTGLDRVTIHGVPEADER
                     RVATFALSVQGISAHEAASRLSQAGLYVWSGHYYALNAVDRLGFLDTGGLLRIGFVHY
                     NTDEEVDRALESLAAL"
     gene            complement(5549..>6374)
                     /locus_tag="VFU96_05110"
     CDS             complement(5549..>6374)
                     /locus_tag="VFU96_05110"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5112675.1"
                     /translation="GAFLSRYHFDRLIRATAGEAPNQFRRRILLERAAYRLATGTDGI
                     LEIAVEAGYSSNEAFTRAFRRAYGVAPSTWRDTPGQIQLSSQNDVHFHPPGGLRVPAR
                     AEGNEMNLVMRMVEHHVWLVGELIAIAESLDDEELDAPIALPFAGIDDDPTVRSLLSR
                     LIGQMDMWNSVIAGRAYDWSVEDREPITSMRARLDRAGSDFLDEVHRVVEEARLDETF
                     IDAHSEPVEVFTYGGLIAHVLTFAAHRRLLVLGALESAGITSLGYGDPRKWVAEAV"
BASE COUNT         1197 a         2052 c         2040 g         1085 t
ORIGIN      
        1 ggaaggcctt catccagcag aacgccgacg acgtccgatt cctggacatc tgagctatga
       61 cccagcagaa cccacccgcc gaaggcgtgc cccaggtcga gatcaccgac gagatggagc
      121 agagctttgt cgactacgcg atgtcggtca tcgtctcccg ggccctgccc gatgtgcgcg
      181 acggtctcaa accggtgcag cgacggatca tctactcgat gttcgagaac aacatcacct
      241 cgggctcggc ccaccgcaag tgctcgaagg tcgtcggtga cgtgatgggg aactaccacc
      301 ctcatggcga cacggccatc tatgacgccc tggtccgcat gggacaggat ttctcgatgc
      361 gccatctgct catcgaccca cagggcaact tcggcagcat cgacgacccg ccgggcgccg
      421 ccaggtacac cgagtgtcgg ctcaccaagt tgtccaacca tctcctcgaa gggatcaggg
      481 aggacaccgt cgacttcgag gacaactact cgggggagat gcagcagccc acggtgctcc
      541 cggcccggtt ccccaacctg ctcgtcaacg gctcccaggg gatcgccgtt ggcatggcga
      601 ccaacatccc gccccacaac ctgaatgaga tcatcgatgc ctgcacccac gtcatcgaca
      661 accccgacgc gaccaccgag gagctattgc agttcgtcaa gggacccgat ttccccaccg
      721 ggggcttcat cgtcggaacc ggtggcatac gggacgccct gaccaccggg cgaggctcgg
      781 tcaagatccg agcggtctgc gacgtccagg agatccgcaa gggccgtacc gccatcatcg
      841 caacggagct gccgtaccag gtctccatcg agcggatcca ggccaagatc agggagctgg
      901 tcgagaacaa gcgtctcgag gggatcgctg acatccgcaa cgagagctcg gaccgggtgg
      961 ggatccggct cgtgatcgag ctgaagagcg gcgccgtgcc ccaggtagtg ctcaaccagc
     1021 tgttcaagaa cacgccgctg caggactcct tcgggtacaa catggtgtct ctcgtcgatg
     1081 gggtgccacg caccctcgac ctcgccgaga tggtgggcta ctacctcgac caccagatgg
     1141 aggtggtgga gcggaggact cgattccgtc ttgccgaggc gcaggcccgg gcgcacatcc
     1201 tcgaaggcct catcatcgca gtcgacaaca tcgacgaggt gatcaagatc atccgaggct
     1261 cagccgacac cgccgatgcc cgggccaacc tgatcgatcg gttcgagttg agcgagatcc
     1321 aggcaaacgc tgtcctcgac atgcccctgc ggcggctcac cgccctcgag gtggacaagc
     1381 tccgatccga gctggccgag ttgcacgacc tcatcaccga gttggaggcg atcctggccg
     1441 atcccaagcg gcgctgggcg atcatccggg acgagctgtc cgagatcaag gagaagtacg
     1501 gcgagagccg gagaagccgg gtcatacccg acgagggcga tctgtccctc gaggacctca
     1561 tcgccgacga cgagttgatc gtgacggtct cctccgccgg ctacatcaag tcggttcccg
     1621 cctccaccta caagacccag gggcggggtg ggcgaggggt caaggctgcc gaggtccagg
     1681 agaccgacgt gatccagcat ctggtgcaca cgacggcgca cgcctatctg ttgttcttca
     1741 ccaacaaggg gctcgtccat cgcatcaagg cgcatggcat cccgaggcag tcccggaccg
     1801 ccaagggggt tctcgcccaa tcggtcctac ccctcgagcc tgaggagcgg gtgcaggcga
     1861 tcatcgacac cagggactac gagacggggc gctatctggt gatggtgacc aagcagggga
     1921 tcgccaagaa gaccgagttc aaggagtacg aatcccggaa tcagacgttg gtggccatca
     1981 agctggtcga tggcgatgag ttggtgtcgg tgcggaccac caacggcgaa aacgacgtgc
     2041 tcatcttcac caaggagggg atggggatcc gtttcaagga ggccgacctc cgcagcatgg
     2101 gccggggcac ccagggggtg cggggcatcc gtctccgtga cggcgaccag gtggttgctg
     2161 cggtgtcgaa cgtcgatggc gacgaggtgc tgctggtcac atctgccggg tacgggaaga
     2221 ggaccgccat cggggagttc cgtcgtcaga cgcggggcgg catcggagtc aaggccttca
     2281 agctgacccg ggtccggggc aatttggtgg gggggcgcgc ggtcaccaaa gacgacgatg
     2341 tgttcctgat ctcctcggcc ggcatcggga tcaggacccc ggtggccacg atcagccggc
     2401 agaagcggga ttcgaccgga gtccgggtca tccatgtcgg cgacggcaat gagttgtcgg
     2461 cgctggccat cgtctccgac gaagtcgagt gagggaaccc tgagccgatt cgtgtcgttg
     2521 ggactaatgc tgtcttcctt tagactgccc cggtaatcct caggagccct atggcaactg
     2581 ttcgtcgcgt gagacgcatc atccgaaaga tcgacccctg gaccgcgctc aaagtgtcgg
     2641 ccgtggtttg ggccgtgctc gccctcgccc tcgtcttggg tggagtgatc ttctggtccg
     2701 ttctcgaacg cgccggcctg ccccagcgtc tcaccgattt catggtcgag atcaccttga
     2761 tcgacgaggg tgcacaacca ttcgccaatg tcgagcagtt cttccgggtg atggtcttcg
     2821 gttcggttgt ctggtgggtg acgatgactg gcgccacagt ggcgggtgca atcgtctaca
     2881 acctcgtgtc cgacgtggtg ggcggtctcg aagtggtcgt gctggaggag accctcaacc
     2941 cggtggtcgc ccccgccccg tcacaggtcc tccacccccc gaccgactac cggcccggca
     3001 acggctcggt gactcctcct cccgaccacg ccgatatccc caccgaagag agccctcggg
     3061 tcctcgaccc gcgcctctga gggccgtcag ccctcctcgg aggcatcgat gacacaaaac
     3121 tggttgccgt ccgggtcagc gagcaccacg tagtcggcat cctcttcgta ggtccattcc
     3181 acccttcggg ctccgagacc gatcagtcgg tctacctcgg cactctggtc ggaggcgtag
     3241 aggtcgaggt gatggcgctg acggggcagt gggtctgagg tcaccagcct gatcgatatc
     3301 cgctgacctg acccttcccg gggcaccagg atggcccaat catcttccgg ctcatagagc
     3361 ggctcgtagt cgagcgcctc accccagaac cgaatcgcct caggcacgtc acgcacaccc
     3421 cagaccaccg acccgagtgt cagcatgatt cgaagctacc ggtcttcggc catgaccaga
     3481 cccatggcgg ccgcggcctg gaggtagacc aaggccgagt cccacaccgg ggcgggttcc
     3541 tcgccgacgg catcgacggc ggcggcgagg gcgattccgg acatgaacgc cccgtcgtcg
     3601 acgatggtga cttgtcgctc ggagccgaca gcgaggcaag cggcgaatcg gtcgggagac
     3661 tgagccagag ccacatgtcg tcccccgaaa gtggcgtcgg ccaccttggc cgtacctacc
     3721 gggtcgggaa atcgcacctg gtgatcgaag ccctcatcca agtcgggatg ggcgacagcg
     3781 atgattctcg gatcgggctc ccgagcaccg agggccagcg ccaacccgac gggggaggcg
     3841 ccccagacga ccacgccttc ggctgcgttg tcgccgtccc acagcaacgg gacacccagc
     3901 ttgcgggctc gcatcggggc tgcgtcgcca ctcccgatga gaaggtcgag ctcggaggca
     3961 tcaggaacga cacgaaagct ccggctgcgt gccggaccga ccacgactac ctcctcgaca
     4021 gagtcgtggg cgctcagact gtgagccgcc ctgacggcgg tgtccccgct ggcgaggacg
     4081 cctaccttca cgaggggttg actggattcc aggccgagtc tgggatgact acttcggaat
     4141 cgcgccggcg tatcaggagg aacgcagcga tggcgccgag gatgatgatg ggagagagtc
     4201 gcttcaccgg ctcagtgtac gggtgtcccc agggttgccc cgcatcgggt cgacgccctc
     4261 agcttcacag cgcggcgaga gactcgaggg cccgatcgac ttcttcatcg gtgttgtagt
     4321 gaacgaatcc gatgcgaagc agtcctcccg tgtcgagaaa gccgagacga tccaccgcgt
     4381 tcagcgcgta atagtgcccc gaccagacat agaggccggc ctggctcagc cggctcgcgg
     4441 cttcgtgcgc cgatattcct tgtaccgaca gcgcgaacgt cgccacccgc cgctcatccg
     4501 cttcgggcac accatggatg gtgacccgat ccagtccggt cacaccctcg atgaacctgg
     4561 tggacagggc gtgctcgtgt tcctggatcc cgcggtaact ctcgaccagc cgggctctcc
     4621 ggttggttcc atcgccgaga ctggccaggt agtcgaccgc ggcggtcaca ccggcaatcg
     4681 actcgaagct ctgggtaccc gtttcgagct tgtcgggcgg gtccgacgga gccgggcgga
     4741 ccttgtagaa ctcgagctcg gcgagccggt cgagtttgcc gaagaggatc ccggtgtggg
     4801 gcccgaagaa cttgtaggcc gaggccacca ggaagtcgca atcgatcgac cggacatcga
     4861 ccagatggtg gggaccggca tggaccgaat ccaggtagac catggcacct gcctcgtggg
     4921 ccagggaggt gagggcagtc acgtcaacga gtgtcccgat cgcgttggat gcgacacaga
     4981 cggcaacgag gcggacgcgg tcatcgagca gatcggccac cactgctggg tccagctcgc
     5041 cggtgatgac atcgaagtgg gcgacgcgaa cctccgctcc cacgtcttca gccgcccgca
     5101 cccacggggt gaagttcgca tcgtggtcca gactcgtcac cacgatggca tcgcctggct
     5161 cccaggcccg ggccagggcc cggctcaccg cgaaggtgat gctggtcatg ttctggccga
     5221 atgagatctc gctcgggtcg gcgttgaaga aatcggccat tgcttctcgg ccaagccggg
     5281 tcgtgtcctc cgcgagttgg gaggctccaa aatcaccgcc tagattgctc accccctcgg
     5341 tcaggaccgc attcatggcg tcgatcacgg ctttgggaac ctgggtgcca ccgggaccgt
     5401 cgagatacgc caccggccgg ccgtgctccc ggcggctcag cgcgggaaac cgggcacgta
     5461 tcgcatcgat gtccaaccgg ctcgtggtca tcgccattgt gtagcagcga tcgagggctc
     5521 caccgatggg aagcggttcg agtttcgctc agacagcctc ggccacccat ttcctggggt
     5581 cgccgtagcc gagcgaggtg atcccggccg attcgagggc accaaggacg aggagccttc
     5641 gatgggcggc aaaagtgagc acgtgcgcga tgagccctcc ataggtgaac acctcgacgg
     5701 gctcggaatg ggcatcgatg aaggtctcgt cgagacgggc ctcttcgacg acacgatgga
     5761 cctcatcgag gaagtcactc cctgctcggt cgagtcgggc tcgcatcgaa gtgatcggct
     5821 ctctgtcctc gaccgaccag tcgtaggcgc ggccggcgat cacgctgttc cacatgtcca
     5881 tctggccgat cagtcgcgac agcagagacc ggacggtggg gtcgtcgtcg atacctgcaa
     5941 atggcagcgc gatcggagcg tccaactcct cgtcgtcgag actctcggca atggctatca
     6001 gctccccgac gagccacaca tggtgctcga ccattctcat caccagattc atctcgttcc
     6061 cttctgctcg ggccgggacc cgaaggccgc caggtggatg gaaatgcacg tcgttttgcg
     6121 atgagagttg gatctggccc ggcgtgtcac gccaggtcga tggggccacc ccataggcgc
     6181 ggcgaaaggc tcgggtgaag gcctcgttcg aggaatagcc cgcttccacc gcgatctcga
     6241 ggatcccatc tgtccccgtg gccaggcgat acgccgccct ttccagcagg atccgccgtc
     6301 tgaattgatt tggggcctca cccgccgtag cccggatcaa tcggtcgaag tgatagcggg
     6361 acaggaacgc gccc
//
LOCUS       DASTXO010000241         4994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_420643, whole genome shotgun sequence.
ACCESSION   DASTXO010000241 DASTXO010000000
VERSION     DASTXO010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4994
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420643"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VFU96_05115"
     CDS             <1..278
                     /locus_tag="VFU96_05115"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU5112676.1"
                     /translation="TFLNEARMLDGVHRARVIAARDAYEAAFRAVIADGVDDGTFRAG
                     TDPKIASIFLLSILNAVERWYRPDGEVDRAGLVDAIVATMVQGLKAG"
     gene            complement(293..1321)
                     /locus_tag="VFU96_05120"
     CDS             complement(293..1321)
                     /locus_tag="VFU96_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020020179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEU5112677.1"
                     /translation="MTAPSYTRADFQTDQEVRWCPGCGDYTILASVQQFLAGLGRPRE
                     EFVFVSGIGCAARFPYYMNTFGMHSIHGRAPAIASGIVATRPDLSVWVITGDGDALSI
                     GGNHLIHALRRNVDMKILLFNNQIYGLTKGQYSPTSEAGKTTKSSPFGSIDYPFNPVS
                     LALGAEATFVARTLDMDRNHTQSLLQAAYEHEGSAFIEIYQNCNVFNDKAFIQLTGKE
                     ERVHNRIDLEAGKPIVFDGGAKAVVMRDGQATIVDSSSVDPKAILVHDPTRPDPAIAF
                     ALSRLSHGPYGPTPLGIFRDVSRPTYEGQMHAQIAAAKEKNGEGDLARLITSQGTWTV
                     KDPSSNGG"
     gene            complement(1318..3195)
                     /locus_tag="VFU96_05125"
     CDS             complement(1318..3195)
                     /locus_tag="VFU96_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015035501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEU5112678.1"
                     /translation="MADEIATAEPSTTTNTETLDKVVIRFAGDSGDGMQVAGSRFTDA
                     SALFGNDLATLPSFPAEIRAPAGTLPGVSSFQVQIADFDILTAGDSPDCLVAMNPAAL
                     KKNLKDLRQGGTLIVNTDAFDERNLAKAGYEGDPLDDGTLDGYRVIKVPMDDLTKEAV
                     KDTGVSGRSVLRSKNFFALGLLAWLFHRPTQPTIDWAQKKFSRTPEVAAANVAAFHAG
                     YTFGEATEAAKITFEVKPAALHPGTYTNVNGNTALAWGLIAASKLSGLPLFYGTYPIT
                     PASDILHELARHKNFGVRTFQAEDEIAGVGSAIGAAFAGSLAVTGSSGPGIALKSEAI
                     SLAFMMELPLIVINVQRGGPSTGLPTKPEQSDLLFSLYGRHGESPIPVLAIASPSDAF
                     ELTIEAARIAIKYMTPVLLLSDNQVANGSEPWMLPDVKGLTPIEVEFAKHGNGPNGTF
                     LPYMRDIDTFARPWAVPGTPGLEHRIGGLEKEAITGNVSYDPGNHQLMTDTRAWKVAN
                     IVNDIPDLEVSGDEDADILVLGWGSTFGSIRAAARRVRLDHDGKVATLHLRHLNPMPQ
                     NLGEILHRYDKILIPELNTGQLRKVIRAEFLVDAKGLNKVAGEPFKISEITEKILEMI
                     K"
     gene            complement(3307..4596)
                     /locus_tag="VFU96_05130"
     CDS             complement(3307..4596)
                     /locus_tag="VFU96_05130"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEU5112679.1"
                     /translation="MSSIDRVERALAAAHAGQVAFNIFTQLDDEAAMDRAALIDGADG
                     DGMPALGLAGVPVGIKDLIDHEGRVTTAGSAFYRHESERSSPVVSRLEDAGAVVIGRT
                     GLHEFAFGFSSENPHFGPVRNPWDPETSPGGSSGGSAAAVAADITPIAIGTDTGGSVR
                     VPAALCGCFGLKVTHGRIPLDGVFPLAGSVDTVGPLASSIDGLDRSYRALSGDQSDWS
                     REDLRLGVPEPWLGQAPTDDEVGSAFDEAVSGLGALGHQVTRIEMPGVLPAPELIDAI
                     AGEVAQVHRRFREEGLPYGPDVAKRVDDCIDTSKDRIEAGRAWQGMIRSRFAAAFDDV
                     DLVLTPTTPVMRKVIGEEMIGDRHYRAVLSWFSALVNHALLPAIALPLAGTGAPPVSL
                     QAIGPLGSETALLHFGRTLEESGLVGFTPAPMASGST"
     gene            complement(4629..>4994)
                     /locus_tag="VFU96_05135"
     CDS             complement(4629..>4994)
                     /locus_tag="VFU96_05135"
                     /inference="COORDINATES: protein motif:HMM:NF037564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 C-terminal domain-containing protein"
                     /protein_id="HEU5112680.1"
                     /translation="DFLGTSVDWTWDEATGTWLLSTDGAESNWRNEDETEGRLGFPVL
                     IGLVSEQYSNNGLPSSHTTGTGQAWVFADGKVVEGTWTRETETEWYTLTDTAGNTIAV
                     PPGQVWISLVPDTGGLTYE"
BASE COUNT          839 a         1539 c         1689 g          927 t
ORIGIN      
        1 gcaccttcct caacgaggcg cggatgctcg atggggtgca ccgtgcccgt gtgatcgctg
       61 cccgggatgc ctacgaagcg gctttccgtg ccgttatcgc cgacggggtg gatgacggaa
      121 cgtttcgggc agggacggat cccaagatcg cctcgatatt cctcctgtcc atcctcaatg
      181 ccgtggaacg gtggtaccgc cccgatgggg aggtggatcg cgccggcctg gtcgacgcca
      241 tagttgccac gatggtgcaa ggcctgaaag ccggctgagg cagaccccgg attcagcccc
      301 cgttggacga cgggtctttc accgtccagg ttccctggct ggtaatcagc cgggcgaggt
      361 cgccttcacc attcttttcc ttggcggccg cgatctgggc atgcatctgt ccctcgtacg
      421 tgggacggct cacatcgcgg aagatgccga gcggagtcgg accatatggc ccatgcgaga
      481 gcctgctcag ggcgaatgcg atcgccgggt cgggccgggt cgggtcatgg accaggattg
      541 ccttggggtc gacgctcgat gagtcgacga tggtcgcctg gccatccctc atcaccaccg
      601 ccttggcccc gccgtcgaac acgatcggct tgcctgcctc caggtcgatc cggttgtgga
      661 cccgctcctc cttgccggtc agctggatga aggctttgtc gttgaagacg ttgcaattct
      721 gatagatctc gatgaacgcg gatccctcgt gctcgtaggc agcctggagc aggctctggg
      781 tgtggttccg gtccatgtcg agcgtgcgag ccacgaaggt cgcctcggcg ccaagcgcca
      841 gtgacaccgg gttgaagggg tagtcgatcg agccgaacgg cgaggacttc gtggtcttcc
      901 cggcctccga tgtcggtgag tactgcccct tggtcaaccc atagatctgg ttgttgaaga
      961 gcaggatctt catatcgaca ttgcggcgga gggcgtggat cagatggttg ccaccgatgg
     1021 agagcgcatc tccgtcaccg gtgatgaccc aaactgacag gtcgggccgg gtggcgacga
     1081 tccctgaggc gatggccggg gcgcgtccgt ggatcgagtg catcccgaag gtgttcatgt
     1141 agtaggggaa tcgggcggcg cacccgatgc ccgagacgaa gacaaactcc tcccggggcc
     1201 gtccaagacc ggcgaggaac tgctgaaccg aggcgaggat ggtgtagtcc ccacatccgg
     1261 ggcaccagcg aacctcctga tcggtctgga agtcggcgcg cgtgtatgac ggggcggtca
     1321 cttgatcatc tccaggatct tctctgttat ctccgagatc ttgaacggct cgccggccac
     1381 cttgttcaaa cccttggcgt cgacgaggaa ctcggccctg atcaccttgc gcaactggcc
     1441 ggtgttcaac tccgggatca ggatcttgtc atagcggtgc aggatctccc cgagattctg
     1501 aggcatcggg ttgagatgac gcaggtggag cgtcgccacc ttgccgtcgt ggtccaatcg
     1561 caccctgcgg gcggcggcac ggatcgatcc gaaagtcgat ccccagccca gcaccaggat
     1621 gtcggcgtcc tcgtcgccgg acacctcgag atcggggatg tcgttgacga tgttcgccac
     1681 cttccacgct ctggtgtcgg tcatgagctg gtggttgcca gggtcatagg acacgttccc
     1741 ggtgatcgcc tctttctcca gaccaccgat ccggtgctcc aacccgggag tgcccggcac
     1801 cgcccagggg cgggcgaagg tgtcgatgtc acgcatgtag ggaaggaagg tgccgttggg
     1861 gccgttgccg tgcttggcga actcgacctc gatcggagtc aggcctttga cgtcgggcag
     1921 catccagggc tcggacccgt ttgcgacctg gttgtcgctc aggaggagga ccggggtcat
     1981 gtacttgatt gcgatcctcg ctgcctcgat ggtcaactcg aaggcgtccg agggcgaggc
     2041 gatcgccagc accgggatcg gagactcgcc gtgacgcccg tagagcgaga acaggaggtc
     2101 ggattgctcc ggcttggtgg gcagaccggt cgatgggccg ccacgttgga cgttgatgac
     2161 gatgaggggc agctccatca tgaaggcgag ggagatcgcc tcgctcttca gcgcgatccc
     2221 cggaccggat gagccggtga cggccaggct tccggcgaag gcggcgccga tggcgcttcc
     2281 gaccccggcg atctcgtcct ccgcctgaaa ggtgcgcacc ccgaagttct tgtgacgggc
     2341 cagctcgtgg aggatgtctg atgccggagt gatcggatag gtgccgtaga acaatgggag
     2401 gccggagagc ttggaggcgg cgatcagccc ccaggccagt gcggtgtttc cattgacgtt
     2461 ggtgtaggta cccgggtgca gggcggcagg cttgacctcg aatgtgatct ttgctgcctc
     2521 ggtggcctca ccaaaggtgt atccggcgtg aaatgccgcg acgttggcgg cagccacctc
     2581 gggggtgcgc gagaacttct tctgcgccca atcgatggtc ggctgggtgg gtcgatggaa
     2641 caaccaggcg agcaggccca aggcgaagaa gttcttggag cggagcacgc ttcggcccga
     2701 caccccggtg tccttgactg cttccttggt gagatcgtcc atgggaacct tgatcacccg
     2761 atatccgtcc agggtgccgt cgtcgagagg gtcgccctca tacccggcct tggccaggtt
     2821 gcgctcgtcg aaggcgtcgg tgttgacgat cagggttccg ccctgacgca ggtctttgag
     2881 gttcttcttc aacgccgccg ggttcatcgc gaccaggcag tcaggcgagt ccccagcggt
     2941 gaggatgtcg aagtcggcga tctggacttg gaagctcgac accccgggga gcgtccctgc
     3001 cggggcgcga atctcggccg ggaaggaggg aagggtcgcc aggtcgttcc cgaacagggc
     3061 actggcgtcg gtgaaccgtg atccggctac ctgcatcccg tcgccggagt ctccggcgaa
     3121 tcggatcact actttgtcga gggtctcggt gttggtcgtg gttgagggtt cagctgtggc
     3181 gatctcgtcg gccactttgg gtcacatcct tgggttgtca tcagtacgcg tctcgccaga
     3241 gccttgaatc gaggttctgg tgagaagacc gcatcccggt tgccgcgtca tctcagttta
     3301 tcgacgtcat gtgctacccg aagccattgg ggcaggggtg aacccgacca ggccggactc
     3361 ttccagagtt ctaccgaaat ggaggagagc tgtctctgag ccgagcggcc caatggcctg
     3421 gagggacacc ggcggggcac ccgtgccggc gagaggaagg gcgatggcgg ggagcaaggc
     3481 gtgattgacc agggcgctga accaggagag gacggcccgg taatggcggt caccgatcat
     3541 ctcttcgccg atgactttcc gcatgaccgg ggtggtgggt gtgaggacga ggtcgacgtc
     3601 gtcgaacgca gcggcaaacc ggctccggat catcccttgc caggccctgc ctgcctcgat
     3661 cctgtccttg ctggtatcga tacagtcgtc cactcgcttc gccacgtcgg gcccgtaggg
     3721 gagaccctcc tcgcggaatc gacggtgaac ctgggcgacc tcgccggcga ttgcatcgat
     3781 cagctcgggt gccggcagca cgcctggcat ctcgattcgg gtcacctggt gcccgagggc
     3841 cccgaggcca ctcaccgcct cgtcgaatgc cgatccgact tcatcgtctg tcggggcctg
     3901 cccgagccac ggctctggca cgccgagccg gaggtcctcc cgtgaccagt cgctctgatc
     3961 gccggaaagg gcccggtacg agcggtcgag cccatcgatc gaggaggcga gaggcccgac
     4021 ggtgtcgacc gagccggcca gcgggaaaac cccgtcgagt gggatgcgcc cgtgggtcac
     4081 cttcagcccg aagcaaccgc aaagggcggc cgggacgcgc accgatccgc cggtgtcggt
     4141 gccgatggcg atcggggtga tgtcggcggc aaccgccgcg gcggagccgc ccgaggaccc
     4201 tcccggcgag gtctccgggt cccacggatt gcggaccggc ccgaaatggg ggttctccga
     4261 ggagaagccg aaggcgaact catggagacc ggtccgtccg atcaccaccg ctcccgcgtc
     4321 ctcgagccgg ctcaccaccg gagacgaccg ctcggactca tggcgataga aggcagaccc
     4381 ggcggtggtg acccggccct cgtgatcgat cagatctttg atcccgactg gcactccagc
     4441 cagtccgagg gcaggcatgc cgtccccgtc agcaccgtcg atgagggccg cccgatccat
     4501 ggccgcctca tcgtcgagtt gggtgaagat gttgaaggcc acctggccgg cgtgagccgc
     4561 ggcgagagcg cgctcgactc gatcgatgga agacatcgag tcgaagctac tgcaactggg
     4621 tcgccggact actcgtaggt gagaccgccg gtgtcgggaa caagggagat ccacacctgt
     4681 cccgggggaa ccgcgatcgt gttccccgcg gtgtcggtca gggtgtacca ctcggtctcg
     4741 gtctcacgtg tccatgttcc ctccacgacc ttgccgtcgg cgaacaccca cgcctggccc
     4801 gtcccggtgg tgtgcgagct cggcaacccg ttgttcgagt actgctccga gacgaggccg
     4861 atcaagacag ggaagccgag ccgaccctcc gtctcgtcct catttcgcca gttcgattcc
     4921 gccccatcgg tgctgagcag ccaggtcccg gttgcctcgt cccacgtcca gtcgacactc
     4981 gtgccgagga aatc
//
LOCUS       DASTXO010000242         5421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_567822, whole genome shotgun sequence.
ACCESSION   DASTXO010000242 DASTXO010000000
VERSION     DASTXO010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567822"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..556
                     /locus_tag="VFU96_05140"
     CDS             107..556
                     /locus_tag="VFU96_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HEU5112681.1"
                     /translation="MTANDVMMGPIDFLVVEWPAGSPPNGEAFPHVVDLVDRGVIRIL
                     DFSFVEKDEAGNVLEIDIADFDGDGSNDLLVFAGASSGLLGEEDREEVGAALEAGAAA
                     AILVYENTWAAPFATALRKSGAQLVASGRIPVNALIQALDELEAAEA"
     gene            575..862
                     /locus_tag="VFU96_05145"
     CDS             575..862
                     /locus_tag="VFU96_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014441892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEU5112682.1"
                     /translation="MGLIRGVARTAVVAGTATAVSNRVSRRQANRWSEQEAAQYQQQE
                     ADQQAPPPPPAPAESGTDDMIAQLKQLGELYDAGVLTDEEFAAQKAKVMNQ"
     gene            complement(869..1567)
                     /locus_tag="VFU96_05150"
     CDS             complement(869..1567)
                     /locus_tag="VFU96_05150"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEU5112683.1"
                     /translation="MSSQPSGVSDESLAPDVEQVSAGIPIRERYGLMLLLILVGYVLS
                     GVDTSRFVLSLDSILWVTLLLTALWSPGVPKRLRRVGLVATLVVLLTTFSLGLVASES
                     ANGWRLLLVGVAQLSALLAILNRISRHERVTLQTVMGGVAAYALIAFVMAAVFHSLQY
                     FTGTDALNGIVDTGDYIYFSFVTLTTVGYGDITPATELAKRLAVVEAFVGQVFLITLV
                     ARLVSLWGRPLRSG"
     gene            1649..2389
                     /locus_tag="VFU96_05155"
     CDS             1649..2389
                     /locus_tag="VFU96_05155"
                     /inference="COORDINATES: protein motif:HMM:NF022589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HEU5112684.1"
                     /translation="MQSPARIERNELRYSLRGNGRVSLVGSMLPYALGAMAAAPIVLV
                     VSAVIVTKAKRPIAAGLAFVVGAVALDVVFAIIILAIAEATGVDSSSGDLAASVDTIL
                     GAVFVILGVAAVFSKPSPEKEEAQRHRVEGFALAGFGSLFKLGVAVQVINSDALVVYA
                     AGLKEIPLAEPAPGVAAAVIAVIIFLVIMLVPYHLPIDLRAIAPQRSRSILGGMTEWL
                     LARTRLIEIVVGLGLGGIFLFKGLSVLL"
     gene            complement(2398..3993)
                     /locus_tag="VFU96_05160"
     CDS             complement(2398..3993)
                     /locus_tag="VFU96_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5112685.1"
                     /translation="MTTADTPSNSRTPSEGSSEAGGRVLVTLILVAAVANLNLSVANV
                     ALPSIGSAFDSSQTTLNLIAVGYSLGLAASVLYLGAVGDRHGRKMMLLLGVSLAIPAS
                     LLAAYAPSDEILFLARLIGGVSAGMAYPTTLALITALWSGPARTKSIALWSGTGGAIA
                     SLGPLVAGALLLQFWWGSVFLITLPLAAVALFMAYRLVPSHVNETTEPVDNLGGILSV
                     VLVSSLILAINFAPVQGEATLVLSLAIVAIASGAAFLIRQRRAQSPLYDLDVAKRRIF
                     WVAAVAGIIVFGSLMGAMFIGQQFLQNVLGYSTLEAGAAILPAALLMVLVAPRSAKLV
                     EHNGARATLLIGYLFCLFGFLTMLLLWAEGSSYWQVGLGYALMGIGVGFAGTPASHSL
                     TGSVPVTRAGMASGTADLQRDLGGAIMQSIFGALLTAGYAAAAVAAIGSSPEQAQITS
                     SVQSQLTKSFAGAEAVAAQYPQYASEITAAAKASFLQGDQWAYVAGIVAVLLGAAIVF
                     ILFPKKEQEEALLARYAAEDAAS"
     gene            4401..5096
                     /locus_tag="VFU96_05165"
     CDS             4401..5096
                     /locus_tag="VFU96_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5112686.1"
                     /translation="MRILVVEDEQDLASAIADSLRRDGYAIDIAPTANSAMTLASVNS
                     YDVVCLDLNLPDGDGLDVCRRMLTEPGRGYDLTSTPKILMLTARGGVDDRIRGLDEGA
                     DDYLVKPFSLGELSARIRALLRRDTSTGSSVITYAGIEMDTARHEVMFHGEAVALTVK
                     EYSLLRWFLLHPEEIHSAERLLEHVWDEHADPFTNTVRMTLSNLRRKLSSAGVVPPII
                     TFPGRGYMLRAES"
     gene            5093..>5421
                     /locus_tag="VFU96_05170"
     CDS             5093..>5421
                     /locus_tag="VFU96_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112687.1"
                     /translation="MTRSRIGLVVVLMAPAVLMAFVVMVVTVALLVSHEPWADARTGI
                     AQVGDDVAVVVVAEEGTIDGLIDSGLLAPALALFPALVVAWIVSGRVRRVVLRARADV
                     EAADVER"
BASE COUNT         1033 a         1673 c         1751 g          964 t
ORIGIN      
        1 cacctgatcc aggtgaaaag tcctcatcct ccacccggta cgatggagga tcgtaggatc
       61 gcaagcaaca gtctgtcttc gagggacagc gaacgagagg acgtccatga ccgcaaacga
      121 cgtaatgatg gggcccatcg actttctggt cgtcgagtgg cccgccgggt cgccaccgaa
      181 tggcgaggct tttccccacg tggtcgatct ggtcgaccga ggtgtgattc ggatcctgga
      241 tttctccttc gtggagaagg atgaagcggg gaacgtcctc gagatcgaca tcgcggattt
      301 cgacggagat ggcagcaacg acctgttggt gttcgccgga gcgtcatccg gcttgctcgg
      361 tgaggaagat cgtgaagaag tgggagccgc actcgaagcc ggagctgccg ccgccatcct
      421 cgtatacgag aacacctggg cggcgccatt cgccacggct ctgcggaaga gcggagcaca
      481 gctggtcgct tccggtcgaa tccccgtcaa tgcactgatc caagccctcg acgagctcga
      541 agccgccgag gcctgacccg aagggaatga gaacatggga ttgattcgag gtgtagcccg
      601 gaccgcagta gtcgccggga ccgccaccgc tgtctccaac agggtgtcgc gtcgtcaggc
      661 caaccggtgg tcggagcagg aagcggctca atatcagcag caggaggccg atcagcaggc
      721 tccgccaccg cctccggccc ccgctgagtc cggcacggac gacatgattg cgcagctgaa
      781 gcagttgggc gagctctatg acgcgggcgt cctcaccgac gaggagttcg ccgcacaaaa
      841 ggccaaggtg atgaatcagt aggggttctc accccgatcg gagggggcga ccccacaacg
      901 agaccaggcg ggcgacgagc gtgatcagga acacctgtcc cacaaacgcc tcgacaactg
      961 ccagtcgctt ggcgagctcg gtggccggcg ttatgtcgcc gtaaccaacg gtggtcagcg
     1021 tgacgaacga gaagtagatg tagtcaccgg tatcgacaat gccgttgagg gcgtccgtgc
     1081 cggtgaagta ctggaggctg tggaagaccg ctgccatcac gaaggcgatc aaggcatagg
     1141 cggccacgcc tcccatgaca gtctgaagcg tcacccgctc atgtcggctg attctgttga
     1201 ggatggcgag cagcgcagag agttgcgcca cgccgacgag caacaatctc caaccatttg
     1261 ccgactccga tgcaaccagc ccgagagaaa aagtcgtcag caggacgacc agtgtcgcca
     1321 cgagcccgac tctccgaagt ctcttcggca ctccgggcga ccacaacgca gtcaggagca
     1381 acgtgaccca gaggatcgag tcgagcgaaa ggacaaaccg gctcgtgtcg acaccagaga
     1441 ggacgtatcc caccaggatg agcagcaaca tgaggccgta gcgctctcga atagggattc
     1501 ctgccgatac ctgttcgaca tccggggcca acgactcgtc ggagactcct gacggctggc
     1561 tgctcatggc ataccattct gcacacttct cggtcgattc ggccggagtc atgccagggc
     1621 gcggggttgc gaacgccata gagtgcgaat gcagtcgccg gccaggatcg agcgcaatga
     1681 gctccggtat tcgctgcgcg ggaacggccg cgtgagtctc gtcgggtcca tgctgcctta
     1741 cgcattgggc gcgatggccg ccgccccgat cgtcctcgtg gtcagcgccg tcatcgtcac
     1801 caaggcgaag cggcccatcg cggcaggact cgccttcgtg gttggtgccg tggcgctcga
     1861 cgtggtgttt gcgatcatca tcctggccat agccgaagcg accggggtcg actcgtcgag
     1921 tggcgacctg gcggcaagcg tggacaccat tctcggcgcg gtgttcgtga tcctgggagt
     1981 tgccgcggtg ttctcaaagc cctcccccga gaaggaagag gcgcaacgtc atcgtgtcga
     2041 aggattcgcc ctggcggggt tcgggtcgct gttcaagctc ggcgttgctg tgcaagtgat
     2101 caactcggac gccctcgtgg tctatgcggc cgggctcaag gagatccctt tggccgagcc
     2161 ggcacccggg gtggcggcgg cggtcatcgc cgtcatcatc ttccttgtca tcatgctcgt
     2221 cccgtatcac ctccccatcg accttcgggc aatcgcaccg cagcgatcgc ggtcgattct
     2281 gggcgggatg acggagtggc tcctcgcgcg aacgcgtctc atcgagatcg tggtcggtct
     2341 cggcctggga gggatattcc tgttcaaagg gctctcggtc ctgctctgac ctgagtgcta
     2401 gctcgcggcg tcctcggcgg cgtagcgagc gagaagggct tcttcctgct ccttcttggg
     2461 aaagaggatg aagacgattg ccgctcccaa gaggacggcc acgatgccgg ccacgtatgc
     2521 ccactggtcg ccctggagga acgacgcttt cgccgcggcg gtgatctcgc ttgcgtattg
     2581 cgggtactgg gcggcgacgg cctcggctcc ggcgaaggac ttggtgagct ggctctgaac
     2641 tgacgaggtg atctgggctt gttcgggaga cgagccgatc gcggctacgg ccgcggccgc
     2701 gtagccggcg gtgagtaggg ccccgaagat cgactgcatg atcgcgccac caaggtcgcg
     2761 ctggagatct gccgtgcccg aagccatccc ggcccgcgtt accggcaccg agccagtaag
     2821 tgagtgagag gcaggcgtgc cggcgaaccc gactccgatc cccatcaacg catatccgag
     2881 cccgacctgc caataggagc tgccctcagc ccagagcaac aacatggtca agaaaccgaa
     2941 gaggcagaaa aggtatccga tcaacaacgt cgccctggca ccgttgtgct ctaccagctt
     3001 cgccgaacgg ggagcgacga gcaccatcag gagagccgcc gggaggatgg ccgcgcctgc
     3061 ttcgagggtt gagtatccga gaacgttttg caggaattgc tggccgatga acatcgcacc
     3121 catgagcgat ccgaagacga tgatccctgc aacagctgcg acccagaaga tccgacgctt
     3181 ggcgacgtct aggtcataga gggggctttg tgcccggcgt tgcctgatca ggaacgcagc
     3241 acctgaggcg atggcaacga tggcgagcga gagcaccaga gtcgcttcac cctgtaccgg
     3301 tgcgaagttg atggcgagga tcaaactcga aaccagcacg acggaaagga tcccgccgag
     3361 gttgtccacc ggttccgtgg tctcgttcac atggctggga acgaggcgat aggccatgaa
     3421 gagggcgacc gcagccagcg gcagagtgat caggaacacc gagccccacc agaattggag
     3481 cagcagggca ccggcaacca gcggaccgag ggaggcaatg gcaccgccag tgcccgacca
     3541 cagcgcgatc gacttggttc tcgccggccc tgaccagagg gccgtgatca aggcgagggt
     3601 ggtggggtag gccattccag ccgagacacc tccgatcaaa cgggccagga aaaggatctc
     3661 gtcgctgggc gcataggcgg cgagcaggct ggcggggatt gccagggata ccccgaggag
     3721 cagcatcatc ttgcgcccgt gtcggtcgcc cacggctccg agatagagca cggaggcagc
     3781 gagccccagc gagtagccca cggcgatgag attcagggtc gtttgcgacg agtcgaaggc
     3841 gctgccgatc gaaggaagcg ccacattcgc caccgacaga ttcaggttgg ccaccgcggc
     3901 aacgaggatc agcgtcacca ggacacgacc gcctgcttct gacgagcctt cggaaggggt
     3961 gcgagagttg ctcggtgtgt cggctgtcgt catcggattg atcagcccac tgttcccacg
     4021 ggagtcacgg tgtcacctcg cagttcgctt tcggactctg cctcgtctct gtttctagcc
     4081 acggcctgac gtgaccctac gcggcagatc gagccggcgg cccgccagca aatgccccag
     4141 gccctggtaa atcggcacgc cgccaatgcc gttgtcgcgc cgacccaccg atcagagtgg
     4201 tctcttttac gttctctata ggtgcgtccg acatcatttg ggcgtgggcg tggcctcgtt
     4261 caactgcaca tttcgggaac cgcagccctc tctgcgtcgt ctttacggtg atcgcgccgc
     4321 gcgttgccca tgaccttctc aattctccag cctcccagct tgctcacgcg cgccccgacc
     4381 agtcacgggg tccgggtctg atgcggattc tcgtggtcga agacgagcag gacctggcct
     4441 ctgctatcgc cgacagcctg cgccgcgacg gctatgccat cgacatcgct cctacggcca
     4501 actcggcgat gactctcgct tcggtcaact cctacgacgt tgtttgcctc gacctgaatc
     4561 tccccgatgg ggacgggctc gacgtgtgcc gacggatgct gaccgagccc gggcgggggt
     4621 acgacctgac ctcgactccc aagatcctca tgctcacggc ccgcggcggt gtcgacgatc
     4681 ggatccgagg cttggatgag ggcgccgacg actacctcgt gaagccgttc tccttgggag
     4741 agctcagcgc ccgcatcaga gctcttctcc gacgtgacac gtcgactggt agctcggtca
     4801 tcacctacgc cgggatagag atggacaccg cccgccatga ggtgatgttc cacggcgaag
     4861 cggttgctct caccgtgaag gaatactcgt tgcttcgatg gtttctcctc caccccgagg
     4921 agatacacag cgcagagcgg cttctcgagc acgtgtggga cgagcacgcc gacccgttca
     4981 ccaacacggt gaggatgaca ttgtcgaatc tccgccgaaa gctgtcatcc gccggcgtag
     5041 tccctcccat aatcaccttc cccggtcgtg ggtacatgct gagggctgag tcgtgaccag
     5101 gtcccgaatc ggattggtgg tcgtgctcat ggcacctgcc gtgctcatgg cgttcgtggt
     5161 catggtcgtg acggtcgcgc tcctggtgag tcacgagcct tgggctgacg ccagaaccgg
     5221 catcgcccag gtaggggacg atgttgcggt ggtcgtcgtg gctgaggaag gaacgatcga
     5281 cgggctgatc gactccggac tgctggcacc tgctctggcc ctctttccgg cgctcgtcgt
     5341 tgcctggatc gtctccggtc gggtgcggcg cgtggtcctg cgggcacggg ccgacgtaga
     5401 ggccgccgac gtcgagcgtc a
//
LOCUS       DASTXO010000243         2246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1009485, whole genome shotgun sequence.
ACCESSION   DASTXO010000243 DASTXO010000000
VERSION     DASTXO010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009485"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VFU96_05175"
     CDS             <1..383
                     /locus_tag="VFU96_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5112688.1"
                     /translation="DFADYLPRIDAQEITGPFRLGWGMDYPHPQNYWQQLLDSRFVPP
                     SGSNSTFYVNPEFDAKIDEANAEADINNAIPLYQEAAAIACADTPLIPMFYGLNQYGW
                     SDNVGGVYVDAFGNLVYTDLTAGG"
     gene            522..1448
                     /locus_tag="VFU96_05180"
     CDS             522..1448
                     /locus_tag="VFU96_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5112689.1"
                     /translation="MTSYIIRRVGQFVLVFFAATFLIFAMVFAIPGDPIRALAGEKPM
                     PDSLRQTLVQQFHLDEPFLAQYWHYMTGVIQGDLGTSFNDRPVADIIKEAFPKTLRLA
                     IYAFIIEMVIGVTAGVWAGIRKDKFLDSLVKVSTVAVISVPIFVLGFMAQLLIGVRLG
                     WLPVTAGNPPSLYGYLLPAMVLAAVSLAYVARLTRSSVVENLRSDYVRTAKAKGLSQR
                     RVIGVHTFRNSLIPVVTFLAIDLGALMGGAIVTETIFNIPGIGRAVYQATLSQEGAVV
                     VGIVTVLVIIYAVANLVVDIMYAVLDPRIRYD"
     gene            1441..>2246
                     /locus_tag="VFU96_05185"
     CDS             1441..>2246
                     /locus_tag="VFU96_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5112690.1"
                     /translation="MTDTTNKTPTEILAPDVTTPEVVPAQAPKQGKPTSLWTDAWKEL
                     RRKPSFIISAVIIIIMVVMAIFPQLFTSVDPRDCSLIDALQTPNAEHRFGTDLQGCDY
                     YARTVYGARTSVSIGLVVTFFATLIAVTLGSLSGYRGGWFDSVMSRITDIWFAIPLIL
                     AGIVFLNALGSKGFWTVCAVLIVFGWPTMLRLMRSSVLSVRELDYIDAARALGASDWR
                     IITRHVLPNGITPVIVYATITVGVIISAEATLSFLNVGLQLPAISWGLMIS"
BASE COUNT          400 a          736 c          666 g          444 t
ORIGIN      
        1 tggactttgc cgactacctg ccgcgtatcg acgcccagga gatcaccggc ccgttccgtc
       61 tcggctgggg tatggactac ccgcacccgc agaactactg gcagcagctg ctggacagcc
      121 gattcgtgcc cccgtcgggc tcgaacagca cgttctacgt caaccctgag ttcgatgcca
      181 agatcgacga ggccaatgcc gaggccgata tcaacaacgc catccccctc tatcaggagg
      241 cggcggcgat cgcctgcgcc gacaccccgt tgatcccgat gttctacggg ttgaaccagt
      301 acggctggag cgacaacgtc ggaggggtct atgtggacgc cttcggcaac ctggtctaca
      361 ccgacctgac ggcaggcggc tgaaaaccga tgacggcggc ggcaacgtcg ccaaacgtgg
      421 agcggcatcc tcgggatgcc gctccacact atggttccat tcggtgagga tgggtgcttc
      481 ggccttcggg gcggaggagg cgattgggtg aagactgacc gatgacgagt tacatcatcc
      541 ggcgggtcgg gcaattcgtc ctcgtgttct tcgccgcgac attcctcatc tttgccatgg
      601 tcttcgccat tcccggagac ccgatacggg cgctcgccgg cgagaagccg atgcccgact
      661 ccctgcgaca gactctcgtc cagcaatttc atctggacga gccgttcctg gcccaatact
      721 ggcactacat gaccggtgtg atccagggtg acctgggcac cagcttcaac gaccggcccg
      781 tcgccgacat catcaaggag gcgttcccca agacccttcg cctcgccatc tacgccttca
      841 tcatcgaaat ggtgatcgga gtcacggcgg gggtttgggc cgggattcga aaagacaaat
      901 tcttagattc actggtgaag gtgagcacgg tcgcggtgat ctccgtgccc atcttcgttc
      961 tgggcttcat ggcccagctc ctgatcgggg tgagactggg atggctcccg gtgacggcgg
     1021 gcaatccacc cagcctctac gggtatctgc tccccgcgat ggtcctcgcc gcggtgtcgc
     1081 tggcctatgt ggcccggctc acccgctcca gtgtcgtgga gaaccttcgc tcggactatg
     1141 tccgcaccgc caaggccaag ggcctcagtc aaaggcgagt catcggtgtc cacacgttcc
     1201 ggaactctct catcccggtg gtcacgttcc tcgccatcga cctcggcgcg ctgatgggcg
     1261 gggcgatcgt caccgagacc atcttcaaca ttcccgggat cgggagagcc gtctaccagg
     1321 cgacattgtc tcaggagggc gcagtcgtcg tcgggatcgt gaccgtgctc gtgatcatct
     1381 atgccgtcgc caatctggtg gtggacatca tgtatgcggt gctcgatccg aggatcaggt
     1441 atgactgaca cgaccaacaa gacgccgacc gagatcctgg cgccggatgt caccactccg
     1501 gaggtggtcc ccgcccaagc accaaaacag gggaagccga ccagtctctg gaccgacgcc
     1561 tggaaggagc tgagacgcaa gcccagcttc atcatctcgg cggtgatcat catcatcatg
     1621 gtggtgatgg cgatcttccc ccagctcttc acctcggtcg acccccgaga ctgcagcctc
     1681 atcgacgctc tccagactcc gaacgcagag catcggttcg gaaccgacct gcagggttgc
     1741 gattactacg cccgcacggt ctacggggcc cgcacctcgg tgtcgatcgg gttggtcgtc
     1801 accttcttcg ccacactcat cgcggtgacc ctcggctctc tctccgggta caggggggga
     1861 tggttcgact cggtcatgtc ccggatcacc gacatctggt tcgccatccc gctgatactg
     1921 gccggcatcg tcttcctcaa tgccctgggc agcaagggct tctggacggt ctgcgcagtc
     1981 ctcatcgtgt tcgggtggcc cacgatgctc cggctcatgc gatcgtccgt gctgtcggtg
     2041 agggagctgg actacatcga cgcggcccgg gcgctcggtg ccagcgactg gcggatcatc
     2101 accagacacg tattacccaa cgggatcaca ccggtgatcg tctacgccac gatcacagtc
     2161 ggagtgatca tctccgccga ggcgacgctc tcgttcctga acgtcgggct ccagctgccc
     2221 gccatctcct ggggcctcat gatctc
//
LOCUS       DASTXO010000244         5190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_494336, whole genome shotgun sequence.
ACCESSION   DASTXO010000244 DASTXO010000000
VERSION     DASTXO010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494336"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1400)
                     /locus_tag="VFU96_05190"
     CDS             complement(<1..1400)
                     /locus_tag="VFU96_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEU5112691.1"
                     /translation="MQLDAKVPPGPVEDKWDNHKFSVRLVNPANKRKFKILIVGTGLA
                     GASAAASLAELGYDVEVFTFHDSPRRAHSIAAQGGINAAKNYKNDGDSVYRLFYDTVK
                     GGDYRSREANVYRLAHESLNIIDQATAQGVPFAREYGGLLDNRSFGGAQVARTFYARG
                     QTGQQLLLGAYQALARQVAAGSVKLHTRTEMLDLVVHEGRAVGIVTRDLISGAIESHS
                     GHAVVLATGGYSNVYFLSTNAMACNVSAIWRAHRKGAGFANPSYTQIHPTCIPASDEF
                     QSKLTLMSESLRNDGRIWVPIRIGDERPPNQVPKEERDYFLERMYPAFGNLVPRDVAS
                     RAVKRIVDEGKGVGPMQNGVYLDFHDAIERDGRQVIEERYENLFEMYYRITDEDPYEV
                     PMRIYPAPHYTMGGLWVDYNLMTTIPGLYAIGEANFSDHGANRLGASALMQGLADGYF
                     VLPITIGDYLAGFLNQP"
     gene            complement(1411..2139)
                     /locus_tag="VFU96_05195"
     CDS             complement(1411..2139)
                     /locus_tag="VFU96_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HEU5112692.1"
                     /translation="MSTTIEERRAPWPVAFYRSAVGKKWVMALTGIGIILFVLAHMVG
                     NWKIFLPDVGGVPDIDIYAEALRELLVPFLPAHVALWILRTGLIVAFLFHIHAAYSLT
                     LMNHRARPEDYQGPRTYVAANYASRTMRWSGVIFLAFLLFHLADLTWGIQPVAPETWA
                     RGEVYANFVASFSRAPVTFFYVIAMIALGIHLYHGAWSMFQSLGINHPRFNSLRRYFA
                     IGLAVLVTVGNAIMPLAVYFGFVG"
     gene            2277..2552
                     /locus_tag="VFU96_05200"
     CDS             2277..2552
                     /locus_tag="VFU96_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEU5112693.1"
                     /translation="MQSKLIQALAIGELSWQDYANCRGADADLFFPERGASTRKAKAI
                     CAACEVRAECLDYAVDMGEKFGIWGGLSERERRRVRRERTQRERKVG"
     gene            complement(2557..3021)
                     /locus_tag="VFU96_05205"
     CDS             complement(2557..3021)
                     /locus_tag="VFU96_05205"
                     /inference="COORDINATES: protein motif:HMM:NF022219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061136 - regulation of proteasomal protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome activator"
                     /protein_id="HEU5112694.1"
                     /translation="MEEAVKPDAVEQAPEEERKEPPVSEPTKLIRIASMTRAMLEEVR
                     QAPLDDGGRRRLADVHSRSLDELREILSPELLDEFDEIMVPLDKEGVSEAELRIAQAQ
                     LIGWLEGLFHGIQASLWSQQMAAQSQLAEMQKRALIPPSPPGRSEDQTGLYL"
     gene            complement(3046..4041)
                     /locus_tag="VFU96_05210"
     CDS             complement(3046..4041)
                     /locus_tag="VFU96_05210"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5112695.1"
                     /translation="MTTDAEVAAEEVPAEESLRVGGWRAVARKEIADHILSGRFVVLM
                     GVLGIATAAAVFAASGGIRDVAPNAQGITALFLKLFTVTADPVPFPLIVFVGFLAPLL
                     GIMFGFDAVSGERSQGTLPRLLSQPIHRDEVILGKFVAGLTVVAIMLTALVLFVSGIG
                     IFRLGVVPTPTEISRLAVWLVFTIIYVGFWQALATLCSVLAKRASTSAMIPLGIWLVL
                     TLFGSFIFGAIASVLSPRDGSDAAEIAYMQTVQTVSQLSPVTLFQQVSSVLLDPEVRT
                     VGLVTVEQVDRAIVSELNLTQSLAVVWPQIAILVALTSVMFAAAYIAFMRDEVRA"
     gene            complement(4038..4988)
                     /locus_tag="VFU96_05215"
     CDS             complement(4038..4988)
                     /locus_tag="VFU96_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5112696.1"
                     /translation="MAASTIRSRKLTKTYGEHTVVDRLDLDIEAGEVFGLLGPNGAGK
                     TTTILMLLGLVEPTKGKIEVLGLDPRWNPLQVKRKVGYLPDAVGFYDPMTGRENLKFT
                     ARLNGLDDYEDLISSLLELVGLADAGDQPAGEYSRGMKQRLGVADALVKGPSVLILDE
                     PTTAIDPEGVVEMLALIQRLAKEEGVTVLLASHLLHQVQTVCDRVAIFVRGKVVAQGT
                     PHELAAVDKGGTIRVEFRLGGEPAKGRAALESLGTASGIAPGRVPGSWVADLHAESLA
                     PAVSGLVSKGADVIGVHRLDEDLESIYRRYFEIEEEEVPV"
     gene            complement(4990..>5190)
                     /locus_tag="VFU96_05220"
     CDS             complement(4990..>5190)
                     /locus_tag="VFU96_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112697.1"
                     /translation="TITPSEEAVAGDYVITFTATNEEVDAPEMEIRTTVNPSAIWGFV
                     GIAVIALTLAGLAWVFRRFGRR"
BASE COUNT          933 a         1633 c         1716 g          908 t
ORIGIN      
        1 ggttgattga ggaagcccgc caggtagtcg ccgatcgtga tggggagcac gaagtatccg
       61 tcggccagcc cctgcatcag cgccgacgcg ccgaggcggt tggcgccgtg gtccgagaaa
      121 ttcgcctctc cgatcgcgta gagcccgggg atggtggtca tcaggttgta gtcgacccag
      181 agcccaccca tggtgtagtg cggcgccggg tatatccgca tcggcacctc gtaggggtcc
      241 tcgtcggtga tgcggtagta catctcgaac aggttctcgt agcgctcctc gatcacctga
      301 cgcccgtcgc gttcgatggc gtcgtggaag tcgagataca cgccgttttg catcgggccg
      361 actcccttgc cctcgtcgac gatccgcttc accgcccgcg acgccacgtc tctggggacc
      421 aggttgccaa aggccgggta catgcgctcg aggaagtagt cgcgctcttc cttggggact
      481 tggtttggtg gccgttcatc gccgattcgg atcgggaccc agatccggcc gtcatttcgg
      541 agcgactcgg acatgagggt cagcttcgac tggaactcgt ctgacgccgg gatgcaggtg
      601 ggatggatct gggtgtacga cgggttggcg aatccggcac cttttcgatg tgccctccag
      661 attgcgctga cgttgcaggc catggcattg gtggagagga agtagacgtt gctgtacccg
      721 ccggtggcga ggaccacggc atggccgctg tggctctcga tggcaccgga gatcaggtct
      781 cgggtgacga ttccgaccgc tctgccctcg tggacgacca gatcgagcat ctcggtgcgg
      841 gtgtgcagct tgacgctgcc cgccgccacc tgacgggcca aggcctgata cgcgccaagc
      901 aagagctgct gaccggtctg tccccgggca tagaaggtgc gggccacctg cgcgccgccg
      961 aacgatcggt tgtcgagcag acccccgtat tcccgcgcaa acggcacccc ctgggcagtg
     1021 gcctgatcga tgatgttcag cgactcgtgg gccagccggt agacgttcgc ctcgcggctc
     1081 cgataatcgc cgcccttgac cgtgtcgtag aagagccggt acaccgagtc gccgtcgttc
     1141 ttgtagttct ttgcggcgtt tatcccgccc tgggcggcga tggagtgtgc ccgacgcggc
     1201 gagtcgtgga acgtgaacac ctcgacgtcg tatccgagct cggcgagaga tgccgcggct
     1261 gatgccccgg ccagcccggt tccgacgatc aggatcttga acttccgctt gttggccgga
     1321 ttcaccaggc ggaccgagaa cttgtggttg tcccatttgt cctcgaccgg gccgggcggc
     1381 accttggcgt cgagttgcag tgtcacctcg tcaccccacg aacccgaagt acacggcgag
     1441 gggcatgatc gcgttgccga cagtcaccag gacggcgagc ccgatggcga agtagcggcg
     1501 caggctgttg aaccgagggt ggttgatccc gaggctctgg aacatggacc acgccccgtg
     1561 atagaggtgg atgccgaggg cgatcatcgc gatgacgtag aagaacgtca ccggcgccct
     1621 ggagaagctg gcgacgaagt tggcgtacac ctcgcccctc gcccacgtct cgggtgctac
     1681 cggctggatg ccccaggtga gatcggcgag atggaagagg aggaaggcga ggaagatcac
     1741 cccgctccag cgcatggtcc ggctggcgta gttggcagcg acgtaggtgc gcggcccttg
     1801 atagtcctcg ggccgggctc gatggttcat cagagtgagc gagtaggcgg cgtggatgtg
     1861 gaacaagaag gccacgatca agcccgtgcg caggatccaa agagccacgt gggcggggag
     1921 gaaggggacc agcagctcgc gaagcgcctc ggcatagatg tcgatgtcgg gcaccccgcc
     1981 gacgtcggga aggaagatct tccagttgcc caccatgtga gcgaggacga agagaatgat
     2041 gccgatcccg gtgagcgcca tcacccattt cttgccgacc gccgatcgat agaaggcgac
     2101 cggccagggg gctcttctct cttcgatcgt ggtggacacc ggaactccat tatgccaagt
     2161 cttcgcctgg ggagaatcgt tcggcgcgac aaggagaaat ccgccactcg gggttgacgg
     2221 cgcgcgcggc cggtcttaat gtcaaacgtg taattacacg gttgtgagga gacctgatgc
     2281 agagcaagct catccaagcc ttagctattg gtgagctgtc ctggcaggac tacgccaact
     2341 gccgtggcgc cgatgccgac ctcttcttcc ccgagcgggg ggcttccacc cggaaggcga
     2401 aggcgatatg cgctgcctgc gaggtgcggg cggaatgtct cgactacgcg gtcgacatgg
     2461 gcgagaaatt cggcatctgg ggtggtctca gcgagcggga gcgtcgtcgg gtccggcgcg
     2521 agcgaaccca acgggaacgc aaggtcgggt agagactcag agataaaggc cggtctggtc
     2581 ctcgctcctg ccgggaggcg agggtgggat caacgctctc ttttgcatct ccgccaattg
     2641 cgattgcgcc gccatctgct ggctccacaa cgaggcctgg atcccatgga acagaccttc
     2701 cagccacccg atcagctggg cctgagcgat ccgcagctcg gcctcggaaa caccttcctt
     2761 gtcgagggga accatgatct cgtcgaactc gtcgaggagc tcgggtgaga ggatctcgcg
     2821 cagttcgtcg agtgatcggg aatggacgtc ggcgagacgc ctccgccccc cgtcatcgag
     2881 tggagcctga cgcacctctt cgagcatggc ccgggtcatc gacgcgatac ggatcagctt
     2941 ggtgggctcg gagaccggtg gttccttccg ctcctcctcc ggcgcctgct cgactgcgtc
     3001 cggcttcact gcttcttcca tgacctcagc ctaaggcaaa accgatcagg cccggacctc
     3061 gtcacgcatg aaggcgatgt aggcggcggc gaacatgacc gacgtcaggg ccaccaggat
     3121 ggcgatctgc ggccagacca cggcgaggct ctgggtcagg ttgagctcgg acacgatggc
     3181 ccgatcgacc tgctctacgg tgaccagtcc gacggtgcgc acctcggggt cgaggagcac
     3241 gctcgacacc tgctggaaca gcgtcaccgg cgagagttgg gacacggtct gcaccgtctg
     3301 catgtaggcg atttccgccg catccgaacc atccctcggg ctcagcacgc tggcgatcgc
     3361 cccgaagatg aacgacccga acagggtcag gaccagccag atgccgagcg ggatcatcgc
     3421 cgaggtcgag gcacgcttgg ccagcaccga gcacaacgtg gccagtgcct gccagaaccc
     3481 cacatagatg atggtgaaga ccagccacac ggcaagacgg gagatctcgg tcggggtggg
     3541 caccacgccg agtcggaaga tcccgatccc ggacacgaac aggacgaggg cggtgagcat
     3601 gatcgcgacc accgtcagcc cggcgacgaa cttgcccaga atcacctcgt cacgatggat
     3661 cggctgagag agcaaccgcg gcagcgtccc ctgcgatcgc tcgccgctga ccgcatcgaa
     3721 cccgaacatg atcccgagga gtggagcgag gaatcccacg aagacgatca gcgggaacgg
     3781 gaccgggtcg gcggtgacgg tgaacagctt caggaacagc gccgtgattc cctgggcgtt
     3841 gggggcgacg tcgcggatgc cccccgaggc ggcgaagacg gccgcagccg tcgcgatgcc
     3901 gaggactccc atcaggacga cgaagcgccc cgacaggatg tggtcggcga tctccttcct
     3961 ggctaccgcc cgccatcctc cgactcgcaa ggactcttcg gcgggaacct cctcggccgc
     4021 gacttcggcg tcggtggtca taccggaacc tcctcttcct cgatctcgaa gtagcggcga
     4081 tagatgctct ccagatcctc gtcgagccga tgaacaccaa tcacatcggc acccttggac
     4141 acgagtccgc tcaccgccgg cgccaatgac tccgcatgga ggtctgccac ccatgatccg
     4201 ggcactcgac ccggggcgat accgctggcg gtcccaagag actcgagtgc ggcccgaccc
     4261 ttggcgggct cgccaccgag acggaactcg acccggatgg tgccaccctt gtccactgct
     4321 gccagctcgt gtggtgttcc ctgggccacc accttgcctc tgacgaagat cgcgacccga
     4381 tcgcacaccg tctggacctg gtggagcagg tggctggcga gaagcacggt cactccctcc
     4441 tccttggcca atctctggat cagggcgagc atctccacca caccctcggg gtcgatggcg
     4501 gtggtcggct catcgaggat cagaaccgat gggcccttca ccagggcgtc ggccactccg
     4561 agtcgctgct tcatgcctcg cgagtactcg ccggccggct ggtcgcccgc gtcggcgagg
     4621 ccgaccagct cgagcaggga gctgatcaga tcctcatagt cgtcgagccc gttgagccgg
     4681 gccgtgaact tgaggttctc ccgcccggtc atcgggtcgt agaagccgac cgcatcgggt
     4741 agatagccca ccttccgctt gacctggagc gggttccacc gtgggtcgag gccgagcacc
     4801 tcgatcttgc ccttggtggg ctcgacgagg cctaggagca tgaggatggt ggtggtcttc
     4861 ccggccccgt tggggccgag caacccgaac acctccccgg cttcgatgtc gaggtcgagc
     4921 cggtcgacca cggtgtgctc gccgtaggtc ttggtcagct ttcggcttcg gatggtcgag
     4981 gcggccatgt cagcggcggc cgaatcggcg gaagacccaa gccaggccgg ccagcgtgag
     5041 ggcgatcacc gctatgccca cgaatcccca gatcgccgat gggttgaccg tggtgcggat
     5101 ctccatctcc ggagcatcga cctcttcgtt ggtggcggtg aacgtgatca cgtagtcgcc
     5161 ggccactgcc tcctccgatg gggtgatggt
//
LOCUS       DASTXO010000245         2685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_715460, whole genome shotgun sequence.
ACCESSION   DASTXO010000245 DASTXO010000000
VERSION     DASTXO010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715460"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..408)
                     /locus_tag="VFU96_05225"
     CDS             complement(169..408)
                     /locus_tag="VFU96_05225"
                     /inference="COORDINATES: protein motif:HMM:NF018487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HEU5112698.1"
                     /translation="MPESKRRKPKNRPVSASAVQATVKSKDPSPTWYVALMAGLMVVG
                     VVLVILRFVFEWDQTILIAGLVAIAAGFLMTTNYR"
     gene            complement(513..770)
                     /locus_tag="VFU96_05230"
     CDS             complement(513..770)
                     /locus_tag="VFU96_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112699.1"
                     /translation="MTGNTVLDVLLAVAFGYIIWRISLAVIRMLSTPPPEVDPGDVVA
                     VDQDYRCTVCGAELTMTAVNIGEDNPPRHCREDMVPIWRPG"
     gene            complement(767..1945)
                     /locus_tag="VFU96_05235"
     CDS             complement(767..1945)
                     /locus_tag="VFU96_05235"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEU5112700.1"
                     /translation="MPNAVIVDAVRTPVGRRNGVFKDYHPVDLASRPLEALVERNGID
                     PALIDDVIMGTVSQTGEQAYNVGRNAALAAGYPEEVPGVTIDRQCGSSQQSAHFAAQA
                     IMAGVQDVVVAAGVENMTRVPMGITAHQGPGLPFGARMLERYSQGLVPQGLSAEMIAE
                     KWGIPRQEMDELSLESHRRATRATAEGRFEEQIIPVGVTGDDGRMRTITRDEGIREDT
                     SLEKLAALQPAFKSDGVITAGNSSQISDGAAAVLVMNEDKARALGLTPQARFVAFAMA
                     GVDPVTMLTGPIPATEKVLNRAGLGIDEIDLFEVNEAFAAVTVAWRMEHGGSDPMALW
                     DRTNVNGGAIALGHPLGASGARLLTTLVWELERTGGRYGLQTMCEGGGMANALIIERV
                     "
     gene            complement(1967..2039)
                     /locus_tag="VFU96_05240"
     tRNA            complement(1967..2039)
                     /locus_tag="VFU96_05240"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2004..2006),aa:Ala,seq:tgc)
     gene            complement(2195..2581)
                     /locus_tag="VFU96_05245"
     CDS             complement(2195..2581)
                     /locus_tag="VFU96_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112701.1"
                     /translation="MATMVDERAPIRGRRLATAGLTTLALLLVAGLGIWQLQSADAPA
                     PSVEQAEEFSPEAVRVRNTAGLYVTEAFSPEAVRIANSSGLYVTEAFSPEAVRIANLA
                     DAHPEEAFSPEVVRMRNLESFLVGAN"
BASE COUNT          486 a          908 c          818 g          473 t
ORIGIN      
        1 ccgatcacgt gcacccctgc ctgttcgcgg gcccacccgg cccgccgccg ccgacgaggc
       61 caccgccgac tacgaggccg ccaccgacga cgacgaggcc gccgccgacc acgaccacga
      121 cgacaaccac gaccacgacg acgacggtgc cgaccaccgc tccctgagtc accggtagtt
      181 ggtggtcatg aggaagccgg ccgcgatagc caccaagccg gcgatcagga tcgtttgatc
      241 ccattcgaag acaaaccgga gtatcaccaa caccactccg acgaccatca gtccggccat
      301 cagagccaca taccaggtag gactgggatc cttggacttg acggttgcct ggacggcgct
      361 cgcagacacc ggtctgttct tgggcttccg acgcttcgac tccggcacga gcagcgagat
      421 tagccaatgt cgaagcccga catcgctgag ttacatgcgt gtagttctcc acaccctgtg
      481 gacagacctc tggaaaacta cacctgtgtg attcaacccg gccgccagat cgggaccatg
      541 tcctcccggc aatggcgtgg cggattgtcc tcgccgatgt tcactgcggt catggtcaac
      601 tccgccccac aaacggtgca tcgatagtcc tgatcgacgg caacgacatc cccggggtcg
      661 acctcgggcg gcggtgtgga gagcatgcga atgacggcga gggagatgcg ccagatgatg
      721 tacccgaacg caaccgccag gaggacgtcc agaacggtat tgccggtcac acccgctcga
      781 tgatcagggc gttggccatt cccccgccct cgcacatggt ctgcagaccg tagcgaccgc
      841 cggttcgctc cagttcccaa accagcgtcg tcaagagtcg cgctccgctg gcacccagcg
      901 ggtggcccag ggcgatcgct ccgccgttga cgttggtgcg gtcccagagc gccatcgggt
      961 ccgagccacc atgctccatt cgccaggcca ccgtcaccgc ggcgaatgcc tcgttcacct
     1021 cgaaaaggtc gatctcgtcg atgccgagcc cggcccgatt caagaccttc tcggtggccg
     1081 ggatgggccc cgtcagcatg gttaccggat cgaccccggc cattgcaaag gccacaaaac
     1141 gggcctgtgg agtgagacca agcgccctgg ccttgtcctc gttcatgacc aacacagccg
     1201 cggcaccatc tgaaatctgg gaggagttgc cggcggtgat gacgccgtcg ctcttgaacg
     1261 caggttgcag ggcggccagc ttctcgagag aggtgtcttc ccgtatcccc tcgtccctgg
     1321 tgatagtgcg cattcgcccg tcatccccgg taacgccaac cgggatgatc tgctcctcga
     1381 atcgaccctc ggcagtggcc cgcgttgccc tccgatgaga ctcgagggag agctcgtcca
     1441 tctcctgtcg ggggataccc catttctcgg cgatcatctc cgcggacaga ccctggggca
     1501 cgagtccttg tgagtaacgc tccaacatcc gagctccgaa tggaagaccg gggccctggt
     1561 gcgcggtgat ccccatcggc acccgcgtca tgttctccac gccggccgcg accaccacgt
     1621 cctgaacgcc cgccatgatc gcctgggcgg cgaagtgggc cgactgttga gaggagccgc
     1681 actggcgatc gatcgtgaca ccgggcactt cttcgggata ccccgcggcc aatgccgcgt
     1741 tgcgtccgac gttgtaggcc tgctcgccgg tctggctgac ggttcccatg atcacgtcgt
     1801 cgattagcgc ggggtcgatg ccgttccgct cgaccagcgc ctcgagcggc cgggaggcga
     1861 gatcgacagg gtgatagtcc ttgaagacgc cattgcggcg gccgaccggg gtccgcaccg
     1921 cgtcgacgat cacggcattg ggcatgtcgc tccagtcatc cgacggtgga gacgatggga
     1981 ctcgaaccca cgacccccgg cttgcaaagc cggtgctctt ccaactgagc tacgtccccg
     2041 agagagctca agtctatgac ctgggtctgc cctagtcatg ggtcgcgact ttgggcaagg
     2101 agtagatcac atcgagagag gaggtcggac gcagcggcgc ccggcctcct ccatgcatgc
     2161 cgcactcggc agtgttttcc ctgtcgttgc cggcctagtt cgcgccgacg aggaaactct
     2221 ccaagttcct catccgaacg acctcggggc tgaaagcctc ctccgggtgc gcgtcggcca
     2281 gattcgcgat ccggacggcc tccgggctga aagcctccgt gacgtacagc ccactggagt
     2341 tcgcgatccg gacggcctcc gggctgaaag cctccgtgac gtacagcccg gcggtgtttc
     2401 tgacccgcac ggcctcggga ctgaactctt cggcttgttc cacgctgggg gcgggggcat
     2461 cggctgattg cagctgccag atcccgagtc cggcaacaag taacagggcc agagtggtca
     2521 atcccgcagt tgccaatcgt cgtccccgga tcggggctcg ttcgtccacc atcgtcgcca
     2581 tcagaagctc cttccggtct gtgggccctt gccttggttg aagggccacg ctctgatggg
     2641 caaggtatgg aaacggggga ccgttgacat cggggcatct acgta
//
LOCUS       DASTXO010000246         5009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1415115, whole genome shotgun sequence.
ACCESSION   DASTXO010000246 DASTXO010000000
VERSION     DASTXO010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415115"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1419
                     /gene="lysS"
                     /locus_tag="VFU96_05250"
     CDS             <1..1419
                     /gene="lysS"
                     /locus_tag="VFU96_05250"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEU5112702.1"
                     /translation="ILESGGYPHRYQRSHLAAELHQKFADLAPASDTDTRVTVAGRLM
                     LHRSFGKLQFGTLQDDTGTIQLFVDRATIGDEKADGFARVDLGDWLGATGTIMTTRKG
                     ELSVRADDYVILQKSLRPLPDKWHGLQDIELRSRRRYLDLMVNDEARRVARARATVIS
                     ELRRQFEARGFVEVETPVLLHQATGATARPFATHHHALDMEMYLRIATELYLKRLVVG
                     GMEKVFEIGRIFRNEGIDSTHNPEFTMLEAYQALADYTDMMDLIEEVVSDLTRELIGA
                     TSLTYQGRDLDLSRPFRRASMLELVSESLGTVVDLDTPVHLLTSLARERGIEVEDGWS
                     EARIISEMYEEIVEPEIWDPTFVTHQPIEISPLARRSLTDPRLADRFELVIAGSEYAN
                     AFSELNDADDQRLRFEEQAAARAAGDVEAHPVDHDYLLALEYGLPPTGGLGIGVDRLV
                     MLLTDQAHIREVILFPTLRPEV"
     gene            complement(1432..2424)
                     /locus_tag="VFU96_05255"
     CDS             complement(1432..2424)
                     /locus_tag="VFU96_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU5112703.1"
                     /translation="MDDLQRLIRIPRVWDSLARVEERLLEAASADDAYLTKIAQHLLL
                     AGGKRFRPLLALLAAEFGETRDRRPVEAGVSVELIHVGSLYHDDVIDEADVRRGAVSV
                     NANWNNTVAILAGDFLMARASEVAATHLSQESVRLLAATYAELVEGQTRELQLDFNLG
                     HTVAEYEQVIAGKTASLIRTSARLGAMAADADDDIVDALSDWAWEVGIVFQIADDALD
                     LVADEETIGKPAGSDIREGKFTMAVLEALSGPDGDRVRSLLESSHPYADAVVKDVIEI
                     VRAGGHVDSSLAEASRHLELASRALGRVPDSDAREILEGLGDYLLGRVEAAKLP"
     gene            2525..>5009
                     /locus_tag="VFU96_05260"
     CDS             2525..>5009
                     /locus_tag="VFU96_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018636279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU5112704.1"
                     /translation="MFERFTDRARRVVVLAQEEARLLNHNYIGTEHILLGLLNEGEGI
                     AAQALESLDIDLASVREEVVKIIGQGQQSPSGHIPFTPRAKKVLELSLREALQLGHNY
                     IGTEHILLGLIREGEGVAAQVLQQLGAELQKVRQTVIQLLSGPAGSEQQSSEPAGSGG
                     RESAAGGSTVLDQFGRNYTQLARERKLDPVIGRIREIERVMQILSRRTKNNPVLIGEP
                     GVGKTAIVEGLAQRIIAKEVPDNLQGKNLYSLDLGALVAGSRYRGDFEERLKKVLKEI
                     KSRGDIILFIDEIHTLVGAGAAEGAIDAASILKPMLARGELQTVGATTLEEYRKYLEK
                     DSALERRFQPIQVDEPSVSETIQILMGLKDRYEAHHSVRFTDQAIVSAANLADRYLSD
                     RFLPDKAIDLIDEAGSRQRIHRTSDVPEITAIDEKLSTLRQDKQDAVSRENYERAANL
                     RDEERGLLSDRAVKEASEGDTGWVIDEEEIAEVLAQWTGIPVHRLTEEETARLVHMED
                     ELHKRIIGQHDAITAVSKAIRRTRAGLKDPRRPSGSFIFLGPSGVGKTETAKALAQFL
                     FGQEDALIQLDMSEYMEKHTVSRLVGSPPGYVGYDEGGQLTEAVRRKPFSVVLFDEIE
                     KAHPDVFNTLLQILEEGRLTDAQGRTVDFKNTVIIMTSNLGTRDLHKKAVGFHAGDSE
                     MDYEHMKERVTEELKKSFRPEFINRLDEIIVFHELTMDELKLIVDLMLERVREQLESQ
                     HLDLVLTDDAKVFLGTRGYDPELGARPLRRAIQRLLEDPLSERVLLGEFRAGTTILVG
                     LDAENDELTFEAVAPPDTTDPSATPQPEMA"
BASE COUNT          979 a         1607 c         1605 g          818 t
ORIGIN      
        1 atcctcgagt cggggggcta cccccaccgc taccagcggt cgcatttggc cgcggagctg
       61 catcagaagt tcgcagacct cgctccggct tccgataccg acactcgagt gaccgtggcg
      121 gggcgtctga tgctgcatcg ctccttcggg aagctgcagt tcggcacact ccaggacgac
      181 accgggacga ttcagctctt cgtggaccgg gccaccatcg gagatgagaa ggcggacggg
      241 ttcgctcgtg tcgacctggg ggactggctc ggggccaccg ggacgatcat gacgacccgc
      301 aaaggcgaat tgagcgtgcg ggcagacgac tacgtcattc tccagaagag ccttcgcccg
      361 ttgcccgaca agtggcatgg gctgcaggac atcgagttgc gcagccggcg gcgatatctc
      421 gacctcatgg tcaacgacga agccaggaga gtggctcgtg cgcgtgcaac cgtgatcagt
      481 gagttgcgca ggcagttcga ggcaaggggt tttgtcgagg tggagacccc ggttctcctc
      541 caccaggcga ccggcgccac tgcacggccg tttgccacgc atcatcacgc cctcgacatg
      601 gagatgtacc tgcgcatcgc aaccgagctg tacctgaagc gcctcgtggt cggcgggatg
      661 gagaaggtct tcgagatcgg ccgcatcttt cgaaacgagg gcatcgactc aacgcacaac
      721 cctgagttca cgatgctcga ggcctaccag gcgctggccg actacacgga catgatggac
      781 ctcatcgaag aggtcgtctc cgatctgaca cgcgagttga tcggagctac gagcctcacc
      841 taccaggggc gggacctcga cctgtcacga ccgtttcggc gggcgtcgat gctcgaactc
      901 gtctcagaat cgctgggcac ggtcgtggac ttggacacac cggtccacct gctgacctcg
      961 ctcgcccgcg agcgcggaat cgaggtcgag gatgggtgga gcgaggcccg gatcatctcc
     1021 gagatgtacg aagagatcgt cgagcccgaa atctgggatc cgaccttcgt cacccatcag
     1081 ccaatcgaga tctcgccctt ggcccgccgc agcctcaccg atccccggct tgccgaccgg
     1141 ttcgagttgg tgatcgccgg atcggagtac gccaacgcat tcagtgagct caacgacgca
     1201 gacgaccaga gactcaggtt cgaggagcag gcggcggctc gtgccgccgg ggatgtggag
     1261 gcacaccccg tcgaccacga ctacctcctg gcgctggaat acgggttgcc gccgaccgga
     1321 gggctgggta tcggtgtcga ccgtcttgtg atgcttctca ccgaccaggc gcacattcgg
     1381 gaggtgatcc tgttcccgac cttgagaccc gaagtgtgag tgacggatcg atcaagggag
     1441 cttggcggcc tcgacccgac cgagcaggta gtcgcccaag ccttcgagga tctcacgtgc
     1501 gtcgctgtcg gggacgcggc ccagggccct agaagccaat tcgaggtggc gggaggcctc
     1561 ggccagagaa gagtcgacat gccctccagc ccggacgatc tcgatcacgt ctttcaccac
     1621 cgcgtcggcg taggggtggg agctctcgag gagtgatcgc acccggtcgc cgtcagggcc
     1681 agacagcgcc tcgagcaccg ccatggtgaa cttgccttcc cggatgtccg atccggccgg
     1741 tttgccgatg gtctcctcgt ccgcgaccag atcgagggcg tcgtcggcga tctgaaacac
     1801 gatccccacc tcccaggccc agtcggagag agcatcgacg atgtcgtcgt cggcgtcagc
     1861 ggccattgcc cctaatcggg ctgaggtccg gatcagtgag gccgtcttgc cggcgatgac
     1921 ttgctcgtac tcggcgacag tgtggcccag gttgaagtcg agctgcagct cgcgggtctg
     1981 tccctcgacg agctcggcat atgtcgccgc gaggagccgc accgattcct ggctgagatg
     2041 ggtagccgcc acttcggagg ctcgcgccat caggaagtcg ccggcgagga tggcgacggt
     2101 gttgttccag ttggcgttta ccgagacggc gccccgtctc acgtcagctt cgtcgatcac
     2161 gtcgtcgtga taaagagagc cgacatgaat caactccacc gagacgccgg cctccaccgg
     2221 ccggcgatcg cgtgtctcgc cgaactcggc agcgagcagt gccagaaggg ggcggaatcg
     2281 cttaccgccg gccagtaaca ggtgctgggc gatcttggtc aagtaggcgt cgtcggccga
     2341 tgccgcctcg agaaggcgct cctcgacgcg ggcgagagaa tcccacacgc ggggaatccg
     2401 aatgagcctt tggaggtcgt ccattcgtga cgatggtatt acgtgcgcac cgctcgggaa
     2461 tcatgctcga aggtcaaaga tcacccggtg gtaaagttgc acatgccagg cttgggagtc
     2521 aagagtgttt gagcgcttca cagatcgtgc ccgccgggtc gtcgtattgg cccaggaaga
     2581 ggcgcgcctg ctcaaccaca actacatcgg caccgagcac attctgctcg gcctcctgaa
     2641 cgagggagag ggcatcgccg cccaggcgct ggagagcctc gacatcgacc tcgcctcggt
     2701 ccgcgaagag gtggtcaaga tcatcggcca gggccagcag tctccctccg gccacatccc
     2761 gttcacgccg agggcgaaga aggtcctcga gctcagcctt cgtgaggcac tccagctcgg
     2821 ccacaactac atcggcaccg agcacatcct gctcggcctg atccgggaag gagagggagt
     2881 agcggcgcag gtcctccagc agctcggcgc agagctccag aaggtgcgtc agacggtgat
     2941 ccagctgctc tccggtcccg ccgggagcga acagcagtcc tccgagccag ccggaagcgg
     3001 gggacgggag tcggcggcgg gcggctccac cgttctcgat cagttcgggc ggaactacac
     3061 ccagctggcc cgagagcgga agttggaccc ggtcatcggt cggatccgtg agatcgagcg
     3121 tgtgatgcag atcctgtctc ggcggaccaa gaacaacccg gtgctcatcg gggagcctgg
     3181 cgtgggcaag accgccatcg tcgaaggtct cgcccagcgc atcatcgcca aagaggtccc
     3241 tgacaacctc caggggaaga acctgtactc gttggacctc ggggccctcg tggccggctc
     3301 tcgctacaga ggtgacttcg aagagcgtct gaagaaggtg ctcaaagaga tcaagagccg
     3361 gggcgacatc atcctcttca tcgacgagat ccacacgctg gttggtgccg gggcagccga
     3421 aggcgccatc gacgcagctt ccatcctcaa gccgatgctg gctcgcggcg agctgcagac
     3481 ggttggcgcc accaccctcg aggagtaccg caaatacctg gagaaggact cggcgctcga
     3541 gcgtcgcttc cagccgatcc aggtcgacga gccgtctgtc agtgagacga tccagatcct
     3601 gatggggctg aaggatcgct acgaagccca ccattcggtc cgattcaccg accaggccat
     3661 cgtctcggcg gcgaacctgg ccgaccgcta cctgtccgac aggttccttc ccgacaaggc
     3721 gatcgacttg atcgacgagg cagggagccg ccagcgcatc catcgcacca gcgacgtgcc
     3781 cgagataacc gccatcgacg agaaactctc caccctccgc caggacaagc aagacgcggt
     3841 gtcccgggag aactacgagc gggccgccaa cctccgggac gaggagcgtg ggctgctcag
     3901 cgatcgtgcc gtcaaagaag cgagcgaagg tgacaccggc tgggtgatcg acgaggagga
     3961 gatcgccgaa gtcctcgccc agtggaccgg aatccccgtg catcgactca ccgaagagga
     4021 gacggcgcgg ctcgtccaca tggaggacga gctgcacaag cggatcatcg gacagcacga
     4081 cgccatcacc gcggtgtcaa aggcgatccg acggacccgg gccgggctca aggatcctcg
     4141 tcgtccctcc ggctctttca tcttcctcgg cccctccggg gtcggcaaga ccgagaccgc
     4201 caaggctctg gcccagttcc tcttcgggca ggaggacgcc ctcatccagc tcgacatgtc
     4261 cgagtacatg gagaagcaca ccgtgagccg tctcgtcggc tcgccgcccg gatacgtcgg
     4321 gtatgacgag ggtggccagc tgacagaggc cgtgagacgc aagccattct ccgtcgtgtt
     4381 gttcgatgag atcgaaaagg cacaccccga tgtgttcaac acgctcctgc agatcctcga
     4441 ggagggccgg ctcaccgacg ctcagggcag gaccgtcgat ttcaagaaca ccgtcatcat
     4501 catgacttcg aacctgggga cccgcgatct ccacaagaaa gcggtgggat tccacgccgg
     4561 tgacagcgag atggactacg agcacatgaa ggagcgggtc accgaggagc tcaagaagag
     4621 cttcaggccc gagttcatca accggctcga cgagatcatc gtcttccacg agctcaccat
     4681 ggacgagctg aagctgatcg tcgatctcat gctggaacgc gtacgcgagc agctcgagtc
     4741 gcagcatctc gacctcgtcc tgaccgacga cgccaaggtg ttcctcggga cccggggcta
     4801 tgaccccgag ctcggggcgc ggcccctccg cagggccatc cagcgcctgc tcgaggatcc
     4861 attgagcgag cgcgtcctgc tcggggagtt cagggcaggc acgacgatcc tcgtcgggct
     4921 cgacgcagag aacgacgagc tcaccttcga ggccgtcgcc ccaccggaca ccacggaccc
     4981 ctcggcgacc ccgcaaccgg agatggccg
//
LOCUS       DASTXO010000247         3621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_15586, whole genome shotgun sequence.
ACCESSION   DASTXO010000247 DASTXO010000000
VERSION     DASTXO010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15586"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1401
                     /locus_tag="VFU96_05265"
     CDS             <1..1401
                     /locus_tag="VFU96_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5112705.1"
                     /translation="KRVSSPATFEVRSPLDWDWLLADVSRGDPLTADQAVTAAVDAFP
                     AWARLTPEQRAEPLHRLADLIDANIAMLARVECIDMAMLLESLEARVIHRGAENFRNY
                     ADLAVSYEGRRWSSKGTDNHIERMPAGPAVIITPWNAPWLLSTWKLAPALAAGNTVIL
                     KPAEWAPLSASLLADLATESGLPPGVLNVVQGTGEEVGAALVSDRRVRRISFTGSPET
                     ARHIGETAGRNIVPFTAELGGKGPLVVFADADLDAAARKAAGQYDDAGQTCLAGTRLL
                     VEASASTEFLELFNAHTDAHILGDSRDPRTTVSPLIHPDHLARVEGFVDRARRHGDRV
                     LRGGERWRQDGLWYEPTLIEPLDNDSEIVQREVFGPVLTYQTFADEGEAVALANSTSY
                     GLSGIVYTGSEERSERIGRGIRAGTVWVNTYLVRDLTAPFGGIGISGIGREGGDYALD
                     FHSDLKTVQVSQGTVT"
     gene            complement(1406..2860)
                     /locus_tag="VFU96_05270"
     CDS             complement(1406..2860)
                     /locus_tag="VFU96_05270"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014445224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEU5112706.1"
                     /translation="MSAILEELKERGKTGLYIGGSWSSGAGELPVVDPATEDVLVDVG
                     LAEASHAGDAVDAAAAALGPWAATPPRERAEILRRTFEAMRRREDDLAELIVLENGKA
                     FPDALGEVRYAAEFFRWFSEEAPRIGGEVRLSPAGDKRIMTVLQPVGVSVMITPWNFP
                     AAMATRKIGPALAAGCTAIVKPASDTPLTTLAVVHLMEEAGLPPGVVNVVVAKGSSGV
                     IEQMLHDPRVRKLSFTGSTEVGQTLLRVAADQVLNVSMELGGNAPFIVFEDADLDAAL
                     EGAMVAKMRNAGEACTAANRFLVHSSVAEKFTSMLAGAMGAMKLGPGIDRSNQVGPMI
                     NERSRDDIARMVQDAVDSGADVAVGASVPDRKGFFYEPTVLAGVSPDAAILDQEIFGP
                     VAPVVEFDSDDEAIGMANDTIHGLIAYVYTGDLARGLRTAEALESGMVGLNRGLVSDP
                     AAPFGGVKQSGIGREGSHEGIEEFLETKYIATSW"
     gene            complement(2857..>3621)
                     /locus_tag="VFU96_05275"
     CDS             complement(2857..>3621)
                     /locus_tag="VFU96_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006008650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HEU5112707.1"
                     /translation="LIPPPPWHYSGEMLTVEYRTDPERVAELLPDGVEPAAEDPGAVA
                     LIWADWQSCGDSFEEVLDPVRSQYKECFAVVRCRWRAETWSRCLFIWVDKDFALVRGY
                     HQGYPKKLGSIWMTRPITVGNAGPRLEAGARFGATLAAADRSLARAVVTLETPGAELG
                     FVNGHPMLHNRWWPAIESDGTESLDELVTMQGYDGEVGDVWSGSAELELLPSPADELS
                     LLAPVEMIGGYYHRVGVSWKGGTTLRRTVPMEVDGQ"
BASE COUNT          622 a         1254 c         1160 g          585 t
ORIGIN      
        1 aaacgcgtgt cctcaccggc gaccttcgag gtgcgctcac cgctcgattg ggattggctc
       61 ctcgcagatg tctcccgggg cgatccgctc acggccgatc aggcagtgac cgcggccgtc
      121 gacgcgttcc cggcttgggc aaggctgacc cccgagcagc gtgcggagcc gcttcatcgc
      181 ctggctgacc tcatcgacgc caacatcgcc atgctggctc gggtggagtg catcgacatg
      241 gcgatgctgc tcgagagcct cgaggcccgg gtcatccacc ggggcgcgga gaacttccgc
      301 aactatgccg atctcgccgt ctcgtatgag ggccgacgct ggtcatcgaa gggcaccgac
      361 aaccacatcg agagaatgcc cgcagggccc gccgtgatca tcaccccgtg gaatgctccc
      421 tggctgctgt cgacctggaa gctggccccg gccctggccg caggcaacac cgtgatcctc
      481 aagcctgccg aatgggcgcc gctttccgca tcgctcctcg ccgacctggc aacagaatcc
      541 gggcttccgc cgggggtgct caatgtcgtc cagggaacag gtgaagaggt cggcgcggcc
      601 ctcgtgtccg atcgacgcgt ccggcggatc tcattcaccg ggtccccgga gaccgcgaga
      661 cacatcgggg agacagcggg gcgcaacatc gtcccattca cggcggagct ggggggcaag
      721 ggacccctgg tcgttttcgc cgatgcggac ttggatgcgg ccgcccgcaa ggcggcgggc
      781 caatacgacg atgccggcca gacctgcctc gccggcacca ggctcctggt cgaggccagc
      841 gcgtcgactg agttcctcga gctcttcaat gcccacaccg atgcccacat cctgggcgac
      901 agccgcgacc cccggacgac ggtcagccca ctgatccatc ccgaccatct ggcccgggtc
      961 gagggcttcg tggatcgggc gcgcagacat ggggaccggg tcttgcgggg tggggagaga
     1021 tggagacagg acggcctctg gtacgagcca accttgatcg agcccctgga caacgactcc
     1081 gagatcgtcc agcgcgaggt gttcggcccg gtgctgacct atcagacgtt cgccgacgaa
     1141 ggggaagcgg tggcactcgc caactcgacc tcgtatggcc tctcagggat cgtctacacc
     1201 ggcagtgagg agcggtcgga gcggattggt cgggggatca gagcggggac ggtctgggtc
     1261 aacacctatc tggtccgtga cctgaccgcg cccttcggag ggatcgggat ctcgggcata
     1321 ggacgggagg gcggcgacta cgcgctcgac ttccactccg acctcaagac cgtccaggtc
     1381 agccagggaa cggtgacgta ggcgatcacc aactggtggc gatgtacttg gtctcgagga
     1441 actcctcgat gccctcgtga ctcccctcgc ggccgatgcc ggactgcttg acaccgccga
     1501 agggggcagc cgggtcggag acgaggccac ggttcagccc gaccatcccc gactcgaggg
     1561 cttcggccgt gcgtaggcca cgagccagat ctccggtgta gacgtacgcg atcagcccgt
     1621 gaatcgtgtc attcgccatg ccgattgcct catcgtcgct atcgaattcg accaccggcg
     1681 cgacaggccc gaagatctcc tggtccagga tcgcggcgtc gggggagacc ccggccaaga
     1741 cggtcggctc gtagaagaat cctttgcggt cgggcaccga ggcccccacc gcaacatccg
     1801 cgcccgagtc gacggcgtcc tggaccattc gggcgatgtc gtcgcgagat cgctcgttga
     1861 tcatcggacc gacctgattg ctccggtcga tcccgggccc gagcttcatg gcccccatgg
     1921 cgccggccag catcgacgtg aacttctctg ccaccgacga gtggaccagg aaccggttgg
     1981 cggcggtgca ggcttcgccg gcgttgcgca tcttggccac cattgcaccc tcgagagcgg
     2041 cgtccagatc ggcgtcctcg aacacgatga acggcgcgtt accgccgagc tccatcgaca
     2101 cattgagcac ctggtcagcg gccaccctga gcaacgtctg gcccacctcg gtcgatccgg
     2161 tgaacgacag cttgcgcacc cgcggatcgt ggagcatctg ctcgatcacc cccgatgagc
     2221 ccttggccac gaccacattc accactcctg gcggcagccc cgcctcctcc atcaggtgca
     2281 cgacggcgag cgtggtcagg ggcgtgtccg atgccggctt gacgatggcg gtgcaacccg
     2341 cggccaaggc gggaccgatc ttgcgggtag ccatggcagc ggggaaattc cacggggtga
     2401 tcatcacgct gaccccgacc ggctggagca cggtcattat tctcttgtct cccgccggag
     2461 agagacgaac ctcgcctccg atccggggtg cctcctcgga gaaccatcgg aagaactcgg
     2521 cggcgtaacg gacctcgccg agagcgtcgg ggaaagcctt cccgttctcg aggacgatga
     2581 gctcggcaag gtcgtcctct cgtcgacgca tcgcctcgaa cgtccggcgc aggatctcgg
     2641 cacgctcgcg tggcggtgtg gccgcccacg gccccagcgc ggcagcggcc gcatcgacgg
     2701 catcgcccgc atggctggcc tcggccaggc cgacatcgac caacacatcc tcggtagccg
     2761 ggtcgacgac cgggagctcc ccggcgcccg aggaccagga gccaccgatg tagagaccgg
     2821 tcttccctcg ctctttgagc tcttcgagga tcgcgctcat tgaccatcaa cctccattgg
     2881 caccgtgcgg cgcagtgtcg tgccgccttt ccatgacact cctacccggt ggtagtagcc
     2941 accgatcatc tccaccggcg cgagcagcga caactcgtcg gccggcgacg ggaggagttc
     3001 gagctcggcc gaaccactcc agacgtcccc gacctctccg tcgtagcctt gcatcgtcac
     3061 caactcgtcg agggactccg tgccatcgga ttcgatcgcc ggccaccaac gattgtggag
     3121 catcggatgt ccgtttacga acccgagttc ggctcccggc gtctcgaggg tgaccacagc
     3181 ccgggccagg gatcggtccg ccgccgccag ggtggccccg aacctcgctc cggcctcgag
     3241 cctgggcccg gcgtttccga cggtgatggg gcgggtcatc catatgctgc cgagcttctt
     3301 gggatagccc tggtgatagc ccctgaccag ggcgaagtcc ttgtccaccc agatgaaaag
     3361 acaccggctc caggtctcgg cacgccaacg gcaacggacc accgcgaagc actccttgta
     3421 ctgggaccgc accgggtcga ggacctcctc gaagctgtca ccacagctct gccaatcggc
     3481 ccagatcaac gccacggcac ccggatcctc ggcggccggc tcgacaccgt ccggaaggag
     3541 ctccgctaca cgctcagggt cggtccgata ctcgaccgtg agcatctcgc ccgagtagtg
     3601 ccagggcggc ggcgggatca g
//
LOCUS       DASTXO010000248         4498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_936635, whole genome shotgun sequence.
ACCESSION   DASTXO010000248 DASTXO010000000
VERSION     DASTXO010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936635"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VFU96_05280"
     CDS             <1..462
                     /locus_tag="VFU96_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006137011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase superfamily protein"
                     /protein_id="HEU5112708.1"
                     /translation="RPTIDAGNDLTEADVDFKLELVLLPVTDVDRSKNFYQEKMGFDL
                     DVDHQPNEEFRVVQLTPPGSACSITFGKGIVDTTPGSVRGLHLVVTDIEAARAELIGR
                     GVEVAEIRHMGANGWEPGPDPSHARYNSFAEIADPDGNTWLLQEVPEAAEV"
     gene            517..1143
                     /locus_tag="VFU96_05285"
     CDS             517..1143
                     /locus_tag="VFU96_05285"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5112709.1"
                     /translation="MQQDTVRVEKLQPDGIDRAAELLSRSFETTPDYTHLFPDPYTRR
                     DALRGFMRAPVRDSQAEGYCWVAADGPAMLGVAAWQAPGRYPWSLGRNLRALPMILSV
                     LRHAPGSFRDLMRFGATIDLHFPDSHPVWYLQVIGVSPEAQGLGVGSRLLEPALGLAD
                     RQRVPCYLETSNEKAVPFYLRAGFEIDSQGVDLMPGGPTYWLMTRPPS"
     gene            1146..2192
                     /locus_tag="VFU96_05290"
     CDS             1146..2192
                     /locus_tag="VFU96_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEU5112710.1"
                     /translation="MPHPARRPITADPVIELVELGFPWPGLDPFIFTVHHFDAYPAGN
                     DDLGPAASLGGRDIGVDFSYLDGWSMYHGDVVPGFPQHPHRGFETVTVVRRGYVDHSD
                     SLGATARYGQGDVQWLTTGSGIMHAEMFPLLDPDGPNPMELFQIWLNLPPDSKMVPAY
                     FGMLWGEDIPRITPSPGVEVEVIAGSIDSSRPPSPPPDSWGSRADSHLAIWHIRIEPG
                     ARWRLPLAPAGVNRMLHCVQGDDVRIGPSRFAARIGARLRPEVETGVENHGEPAAFLL
                     LQGQPIGAPVFQLGPFVMNSADELRQAVDDYHRTQFGGWPWSRRDPVHPRSEGRFAIH
                     ADGRVEHRDMIPAS"
     gene            2192..2611
                     /locus_tag="VFU96_05295"
     CDS             2192..2611
                     /locus_tag="VFU96_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HEU5112711.1"
                     /translation="MAGYSGTPLIKKLGVAEGTTLGILNMPNYYDLELGDLPPDVQVH
                     RGDFPADIFIVFAERSDEAERAFQAAIPSLPPNGAIWVFWPKKSSDVETDLTEQTLRD
                     LFLPTGMVDNKVIAVDETWSGLRFVVRKENRESWPSP"
     gene            complement(2618..3007)
                     /gene="cdd"
                     /locus_tag="VFU96_05300"
     CDS             complement(2618..3007)
                     /gene="cdd"
                     /locus_tag="VFU96_05300"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009645864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU5112712.1"
                     /translation="MNREELVAAARTAAENAYSPYSQFRVGAVVVSTDGETFTGANIE
                     NAAYPSSNCAEATAIHTAVASGVRKIDTLAVACIDAPTVQGSYPCGRCRQIMAEFGVE
                     TVIVAAGHGSDVRVHTLEELLPFRFTL"
     gene            complement(3004..3996)
                     /gene="ftsY"
                     /locus_tag="VFU96_05305"
     CDS             complement(3004..3996)
                     /gene="ftsY"
                     /locus_tag="VFU96_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017626265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEU5112713.1"
                     /translation="MSEWALILGVVALALIVTAVIVVGRRRRTGGESASVETTRPSRI
                     GRSAAVLGISLRRAWGSGLDEHTWEDLEEALLGADVGLESTASVVAGVKAAAPETPEE
                     ARRALATQLRGELGDKNRELHLEGDPAVILVVGVNGTGKTTTIAKLAARLQRSGREVV
                     LAAGDTFRAAAGAQLDTWGGRLGVKVVSGQEGGDPASVAFDAIESARARGDDVVIVDT
                     AGRLHAKKNLMAELGKIHRVASGDRGVVDEVLLVLDATGGQNGVIQVREFSSAVPISG
                     IVLTKLDGTAKGGIVIAIERQLGVPIKFVGLGEGIDDLMPFDPDSFVSDLLEQS"
     gene            complement(4019..>4498)
                     /locus_tag="VFU96_05310"
     CDS             complement(4019..>4498)
                     /locus_tag="VFU96_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013410746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5112714.1"
                     /translation="EIEHRFRDAFGEVAEAYQRYFAVLFPGGRGRIRLADSEDPNSGL
                     MIEAQPLGKKVSQLTLLSGGERSLAALAFLFSIFEARPSPFYVLDEVEAALDDANLRR
                     FLRIVDEFRQRAQLLIVTHQQQTMEAADVLYGVTMEPGGSSQVVRKDMTAIVADVAV"
BASE COUNT          790 a         1528 c         1411 g          769 t
ORIGIN      
        1 cggccgacca tcgacgccgg caacgacctg acggaggctg acgtggactt caagctcgag
       61 ctggttctcc tcccggtcac ggacgtggac cgctcgaaga acttctatca agagaagatg
      121 gggttcgacc tggacgtcga ccaccagccg aacgaggagt ttcgcgtcgt gcaactgaca
      181 ccaccgggct cggcgtgctc aatcaccttc ggaaagggca tagtcgacac gactcccggt
      241 tcggtgcgcg ggctgcacct tgtcgtgaca gacatcgaag ctgctcgtgc cgagctgatc
      301 ggccgcggag tcgaggtcgc agagatccgg cacatgggcg cgaacggctg ggagccgggg
      361 cccgacccca gtcatgcccg ctacaactcc ttcgccgaga tcgccgaccc tgacggcaac
      421 acctggctgc tccaggaagt gcccgaagcg gctgaggtct agccggcacc acgacctggt
      481 ggtttgatcg agcttcgctg cgggtagcac cagtggatgc agcaagacac agtccgagtc
      541 gagaagcttc agcccgatgg catcgatcgg gccgcagaac tgctctcgcg cagcttcgag
      601 accacccccg actacaccca cctgttcccc gacccctaca cgcgtcggga tgcgcttcgt
      661 ggattcatgc gggccccggt tcgcgacagc caggcggagg gctactgctg ggtcgccgca
      721 gacgggcctg ccatgcttgg agtcgccgcc tggcaggcgc cgggacgcta cccctggtca
      781 ctgggacgca atctgcgtgc cctgcccatg atcctctcgg tcctcaggca tgcgcccggg
      841 tcgtttcgtg acctcatgag gtttggagcg acgatcgacc tccactttcc cgacagccac
      901 ccggtgtggt atctccaggt gatcggggtg agcccggagg cacagggtct cggtgtaggg
      961 agccgactcc tcgagcccgc ccttggcctg gccgaccgcc aacgcgttcc ttgctatctc
     1021 gaaacctcga acgaaaaggc ggtcccgttc tacctgaggg ccgggttcga gatcgacagc
     1081 cagggggtcg atctgatgcc cggcgggcct acttactggt tgatgacacg gccaccgtcg
     1141 taggattgcc ccacccggca aggagaccca tcacggcaga ccccgtcatc gaactggtcg
     1201 agttgggctt cccctggccc gggctggacc cattcatctt cacagtccac catttcgacg
     1261 cctacccggc ggggaacgac gacctcggac cggccgcatc actcggcgga cgggacatag
     1321 gggtggactt ctcctacctc gacgggtgga gcatgtacca cggcgatgta gtgcctggct
     1381 ttccccagca ccctcaccgc gggttcgaga cggtgaccgt ggtccgccgc gggtatgtcg
     1441 accactccga ctctcttggc gccaccgccc gatacgggca gggtgatgtg cagtggctga
     1501 ctaccggaag cggaatcatg cacgccgaga tgttccctct tctcgacccc gacgggccca
     1561 acccgatgga gttgttccag atctggctga acctcccacc cgattcgaag atggtcccgg
     1621 cgtatttcgg gatgctgtgg ggagaggaca tcccgagaat cacaccttcg cccggcgtcg
     1681 aggtcgaggt gatcgccggc agcatcgact ccagtcgccc accctcgcct ccgccggact
     1741 cgtggggctc tcgggcagac tctcacctcg ccatctggca tatccgaatc gaaccggggg
     1801 cacgttggcg gctcccgctc gcgccagcgg gggtgaaccg gatgctgcat tgcgtccagg
     1861 gggacgacgt gcgaatcggc ccgagccgat tcgcagcgag gataggagcg aggctccggc
     1921 ccgaggtaga gaccggggtc gagaaccacg gcgagccggc tgcctttctc ctgcttcaag
     1981 gtcagccgat aggtgccccg gtgttccagc tgggcccctt cgtgatgaat tcggccgatg
     2041 agttgcgaca ggcggtcgac gactaccacc gcacccaatt cggtggttgg ccgtggtcac
     2101 gccgcgaccc ggttcacccg cgctccgaag ggcgtttcgc catccatgcc gacgggagag
     2161 tggagcatcg ggacatgatc ccggccagtt gatggcgggc tacagcggca cgccgctcat
     2221 caagaagctg ggcgtcgccg agggaacgac gcttggcatc ctgaacatgc ccaactacta
     2281 cgacctcgaa ctcggcgacc tgccgccgga cgtccaggtg catcgtggcg acttcccggc
     2341 cgacatcttc atcgtctttg ccgagcgctc cgacgaagcg gagcgcgcct tccaggctgc
     2401 catcccctcg ctgccgccga atggggcgat ctgggtgttc tggccgaaga agagctcgga
     2461 tgtggagacc gacctgaccg agcagacatt gcgcgacctg ttcctgccca ccggcatggt
     2521 cgacaacaag gtgatcgcag tcgatgagac gtggtcgggt ctccggttcg tggttcgcaa
     2581 ggagaaccgg gagagctggc cctcgccctg accccagcta cagggtgaag cggaagggga
     2641 gcagctcctc gagggtgtgc accctgacat cggacccgtg acccgcggcc acgatgacgg
     2701 tctcgacccc gaattcggcc atgatctgcc ggcaacgccc gcacggatag gagccctgca
     2761 ccgtcggagc gtcgatgcag gcaacggcca gcgtgtcgat cttgcgcaca ccggaggcga
     2821 cggcggtgtg gatcgcggtg gcctcggcgc agttcgaaga tgggtacgcg gcattctcga
     2881 tgttggcgcc agtgaaagtc tccccatccg tggacaccac caccgcccca accctgaatt
     2941 gggagtaagg cgaatacgcg ttttcggcgg cagtgcgggc tgcagccacc agttcttctc
     3001 ggttcatgac tgctccagga ggtcggagac gaaagagtcc gggtcgaagg gcatgaggtc
     3061 gtcgatcccc tcacccaacc cgacgaactt gatgggcaca ccgagttgac gctcgatcgc
     3121 gatgactatt ccccccttgg ccgtcccgtc gagcttggtg aggacgatgc cactgatcgg
     3181 gaccgctgaa gagaactcgc gcacctggat caccccgttc tgcccgccgg tggcatcgag
     3241 gacgagcagc acctcgtcga ccacgccccg gtcgccagat gcaacgcgat ggatcttgcc
     3301 cagctctgcc atgaggttct tcttggcatg aagccgtccg gcagtgtcga cgatcaccac
     3361 atcgtcccct ctggcccgag ccgactcgat ggcgtcgaac gccacggatg ctggatcgcc
     3421 gccttcctgt cccgagacca ccttcacccc gagccgccca ccccaagtgt ccagttgggc
     3481 gccggcggcg gcgcggaatg tgtcccccgc ggctagcacc acttcccggc cactcctctg
     3541 caggcgggcg gccagtttgg cgatcgtcgt cgtcttgccc gtcccgttga cccctacgac
     3601 caggatcact gccggatctc cctcgagatg caactcgcgg ttcttgtcgc cgagctctcc
     3661 ccgcagctgc gtggcgaggg ccctccgcgc ctcttcgggc gtctcgggag ccgcggcctt
     3721 cacgccggcc actaccgaag cggtagattc gagaccgacg tcggcgccga gcagtgcctc
     3781 ctcgaggtcc tcccaggtgt gttcgtcgag gccgctgccc cacgcccggc gcagggagat
     3841 gcccaggacg gccgcactcc gcccgatccg ggagggtcgc gtcgtctcca cagacgctga
     3901 ttcgccgcca gtgcggcgcc gacgacccac cacgattact gcagtgacaa tcagggcgag
     3961 ggcaaccacg ccgaggatca gtgcccattc gctcattccc atagcgacgc caagctaatt
     4021 acacggcgac gtcggcaacg atcgcggtca tgtcctttcg gaccacctgt gaggacccac
     4081 ccggttccat cgtgaccccg taaagcacat ccgccgcctc catcgtctgc tgctgatggg
     4141 tgacgatcaa caattgggcc ctctgcctga actcgtccac gatgcgaagg aaccgccgca
     4201 ggttggcgtc gtccagggct gcctccacct cgtcgagcac gtagaagggg ctgggcctgg
     4261 cctcgaagat ggagaagaga aaagcgaggg cggccaggct ccgctccccg cccgagagca
     4321 gggtgagctg gctcaccttc ttgccgagcg gctgagcctc gatcatcaac ccggaattag
     4381 ggtcctcgga atcggccagc cggatcctgc cccgaccgcc ggggaagagc accgcgaagt
     4441 agcgctgata tgcctcagcc acctctccga atgcgtcacg gaagcgatgc tcgatctc
//
LOCUS       DASTXO010000249         2543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1673248, whole genome shotgun sequence.
ACCESSION   DASTXO010000249 DASTXO010000000
VERSION     DASTXO010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1673248"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VFU96_05315"
     CDS             complement(<1..370)
                     /locus_tag="VFU96_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5112715.1"
                     /translation="MSDSTPLLQIEGLRASAGDIEILTGVDLTVDQGEIHALMGPNGS
                     GKSTLANVLMGHPSYRVTSGQILYKGEDMTRVSASQRGAAGMFLAFQHPESIPGVSVI
                     QFLRQALSNRTGVDLTVLELR"
     gene            complement(430..915)
                     /locus_tag="VFU96_05320"
     CDS             complement(430..915)
                     /locus_tag="VFU96_05320"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEU5112716.1"
                     /translation="MSDLPEASRRLASSMDDFVMATRVFPEGTKTAEDAARAVGCPVS
                     AIIKSLVFVLIGPDASEEPVVALVPGDLRLDTVKLADVAGVTGSRRATLDEVRSATGY
                     AAGGTPPFGHATPLRVFADHRLRRNDPVWAAAGTPTTVFPISIADLDRLAKPLWADLS
                     E"
     gene            complement(912..1820)
                     /locus_tag="VFU96_05325"
     CDS             complement(912..1820)
                     /locus_tag="VFU96_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5112717.1"
                     /translation="MQFSIMTEPQLGGTYEQLLRAALLAETEGLHSFARSDHLAWQRE
                     TALDATDAFATMGGLARDTHTVRLAVLVTPITFRHPAIIAKNAATIDQMSGGRFDLGI
                     GTGWNDFEHEALGIPFPEAPERWARLEDALGYLEVAFNPDSGTHSGPFYSLDLDVRPK
                     PPGLRLIVGGSGPRRTPALAGTRADEYNIFICPVDEARDKIQVMRDAAGDRVVEATMM
                     GPVTVAGTDAELAELIASAAGRRHITSDELIASWERSGMLFGTPTRLQEKMAALEEAG
                     VQRIYLQWLDLSDYDGLARMVELARG"
     gene            complement(1851..>2543)
                     /locus_tag="VFU96_05330"
     CDS             complement(1851..>2543)
                     /locus_tag="VFU96_05330"
                     /inference="COORDINATES: protein motif:HMM:NF042869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEU5112718.1"
                     /translation="GREHAGPEAASAALFRGAPWVVCDDGAAEDDIETLTTIVRSTGA
                     NPIVMSAKRHDEVVASVSHLPYLLAVTLVNTISRNPDAADLVSGSFRDLTRVASAEAS
                     WWPEVLSSNSASVTSAIDDLVAGLEDLRSRVESGDLDGLAQRLDDARGRRGAMAPHVA
                     MVQVILQDRPGEIAAVGHALQTSKVDVRDLQLRHAVHGGGGVLSLSVRPGEAETLKAA
                     LVKEGFTIEVED"
BASE COUNT          446 a          808 c          844 g          445 t
ORIGIN      
        1 gacgcagttc caggacggtc agatccactc cggtccggtt ggagagggcc tgacggagga
       61 actggatcac cgacacaccg ggtatggact cgggatgttg gaaggccagg aacatcccgg
      121 cagcgccgcg ttggctagcg ctgaccctgg tcatgtcctc tcctttgtag aggatctgcc
      181 ccgacgtgac acgataagaa ggatgtccca tcaggacatt ggcaagagtc gactttcccg
      241 atccgttggg gcccatgagt gcgtggatct cgccctggtc gacggtgaga tccacgccgg
      301 tgaggatctc gatgtcaccg gcagaggctc gcaatccttc gatctggaga aggggagtgg
      361 agtcgctcac cccacaatgg tatttgcact attcccatag gggaaatggg cgggcgaggc
      421 gccggacggt cactcgctga gatctgccca gagaggtttg gcgagacgat cgaggtcggc
      481 gatcgatatc gggaacacag tcgtcggggt gccggcggca gcccaaaccg ggtcgttgcg
      541 acgcagtctg tggtcggcga agacccgaag gggcgtggcg tggccaaaag gcggagttcc
      601 cccggcggcg tacccggtcg cggagcggac ctcgtcgagg gttgcccgtc tcgagccagt
      661 gactccggcc acatctgcca gcttcaccgt gtcgaggcgc aagtcacccg gaaccagggc
      721 gaccacaggt tcctcggagg catcgggacc aatcaagacg aaaaccaagg acttgatgat
      781 cgcggatacc gggcacccca ccgccctggc cgcgtcttcg gcggtcttgg tgccctccgg
      841 aaacaccctg gtcgccatga cgaagtcgtc catcgacgag gcgaggcggc ggcttgcttc
      901 ggggaggtcg ctcatcctcg cgccaactcg accatccgag ccagaccgtc gtagtcggag
      961 agatcgagcc actggagata gatccgctgg accccggctt cctcgagcgc tgccatcttc
     1021 tcctgcaacc gtgtcggagt cccgaagagc atgcccgatc tctcccagct ggcgatcagc
     1081 tcgtcgctgg tgatgtgacg gcgcccggcc gccgaggcga tcaactcggc caactcggcg
     1141 tcggtcccgg caacggtcac aggtcccatc atggtcgcct cgacgacccg gtcaccggcc
     1201 gcgtcacgca tcacctggat cttgtccctc gcctcgtcga cggggcatat gaagatgttg
     1261 tactcgtccg ccctggtccc ggccagggcc ggggtgcgtc tgggcccact accacccact
     1321 atcaaacgta gccctggtgg ttttggcctc acgtcgagat cgaggctgta gaacggtccg
     1381 gagtgggtgc cgctgtctgg attgaacgcc acctccagat atcccagggc atcttcgagg
     1441 cgcgcccatc gctcgggggc ctcggggaag gggatcccaa gggcttcgtg ctcgaagtcg
     1501 ttccatccgg tgccgatgcc caaatcgaat cggccacctg acatctgatc gatggttgcc
     1561 gcattcttgg cgatgatcgc cggatggcga aaggtgatcg gtgtcaccaa cactgccaac
     1621 cgcacggtgt gggtgtcccg ggccagaccc cccatggtgg cgaaggcatc cgttgcgtcg
     1681 agagcggtct cccgttgcca ggccaggtga tcggatcgtg cgaacgaatg gagaccctcg
     1741 gtctcggcga ggagcgccgc tcggagtaac tgttcgtacg tccccccgag ttggggctcg
     1801 gtcatgatcg agaactgcaa gaggctcctg ggctgtgggg gacggggagg ctaatcctcg
     1861 acctcgatcg tgaatccctc tttgaccagg gccgccttaa gagtctccgc ttcgccgggc
     1921 cggacggaga ggctgaggac gccgccgcca ccgtggacgg catgtcgcag ttggaggtca
     1981 cggacgtcca ctttgctggt ctgcagggcg tggccgacgg cggcgatctc gccggggcgg
     2041 tcctgcagga tcacctggac cattgcgacg tggggggcca tcgccccacg gcgccccctg
     2101 gcatcatcca ggcgttgggc caggccgtcg aggtctccag actcgacacg cgatctgagg
     2161 tcctcgagac ctgccacgag atcgtctatt gccgaggtca ccgatgcaga gttggaagag
     2221 aggacctcgg gccaccacga agcctccgcc gacgccactc gggtcaggtc gcgaaagctg
     2281 cccgagacca ggtcggccgc atcggggttg cgcgagatgg tgttgaccag cgtcaccgcg
     2341 agcagatacg ggagatgtga cacggaggcg accacctcgt cgtgtcgttt cgccgacatc
     2401 acgatcgggt tggccccggt cgacctgacg atggtggtca gcgtctcgat gtcgtcctcg
     2461 gcggctccat cgtcacagac cacccatgga gcgccacgga acagcgccgc ggacgccgcc
     2521 tctggcccgg cgtgctcgcg tcc
//
LOCUS       DASTXO010000250         3798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_457278, whole genome shotgun sequence.
ACCESSION   DASTXO010000250 DASTXO010000000
VERSION     DASTXO010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457278"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1219
                     /locus_tag="VFU96_05335"
     CDS             104..1219
                     /locus_tag="VFU96_05335"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004012685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEU5112719.1"
                     /translation="MARSIGILTAGGDSPGLNAAIRAVGKAAQRAYGMHVIGFYDGFL
                     GLMQNRFVRFDSDSLSGILTRGGTVLGTSRAKVDKMEMGDGRRMDMRSLMRDVVDQHD
                     LEALVVLGGDGTMRNALKLEDVGINVLALPKTIDNDVTGTDVTFGFDTAMGIATEAID
                     RLHSTAHSHHRIILAEVMGHEVGWLAMGAGLAGGADVILIPEIPYDIEAIATSIQARK
                     AAGSAFSIIAAAEGAYSREDARLRASLRAEIEGTEGEERRVAKERLTALARSPRTEKL
                     AAALEDATGLESRVTILGHVQRGGTPSPADRLLATRLGTATAAYIADGVHGVLVAARG
                     QSTEPVPLKEIAGVTKFVPLDHPWVHSARSLGIVLGD"
     gene            1212..1787
                     /locus_tag="VFU96_05340"
     CDS             1212..1787
                     /locus_tag="VFU96_05340"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEU5112720.1"
                     /translation="MTDSTLLGAVLAGGASRRMGTDKADVEVSGSTLLVRVASALAEV
                     ADRVVVLGGEREGYESWPDRAPGSGPLAGLATALSRMTEDRALVVAVDNAFVQPGTLA
                     RLASVESDLPVVPVDHAGVRQVTCAVYPRQIAGQAVEEAESGGSVQTLLDRVSFLPVT
                     PDQWETWGEDGRSWYSADTPEALETGLRRFT"
     gene            complement(1794..3029)
                     /locus_tag="VFU96_05345"
     CDS             complement(1794..3029)
                     /locus_tag="VFU96_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEU5112721.1"
                     /translation="MSPRDHKTIIEATFRESYAVVLSGLATRFRDIDLAEEAVQDAMV
                     EALRTWPEQGIPDNPPGWISAVAQRRGIDRIRRRATLMRKASILAGYEKADHDNPPEV
                     VSSGELGDDRLQMLFACCHPSLSTDKQVALTLRTVGGLTTFEIAHAFLVPEPTMAQRL
                     VRAKAKIRDAGIPFQVPTGADLGDRLSAVLAVVYLVFNEGYFATTGGDLVRTELAESA
                     LGLGAVLSQLMPEEAEVLGLQSLMLLQHSRRDARVDDSGRLVLLRDQDRARWDAGAIA
                     QGLDLLERARLLGPSGPYRLQAEIAAQHAIASSPESTDRARIVELYDRLAAIHPAPVV
                     RLNRAVAVFEAHGAREGLEALGDLARDLDGYLGFHLARSEMLRELGDEAEARAELDRA
                     LSLTTNETERRFLEERAAL"
     gene            3127..3651
                     /locus_tag="VFU96_05350"
     CDS             3127..3651
                     /locus_tag="VFU96_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEU5112722.1"
                     /translation="MPVDLPSPTIDPGAFVAPGAHLYGDVTISNGVFVLFGVVIRAEL
                     DRVEVGAYTNIQDNSVLHCDEQIPCVLGERVTVGHAAVVHGALVGDHCLIGIGAKLLN
                     RSRLGEGAWLASGSVLPEGKEIPPWTLGMGIPARPVRELTDAEVERANDGVDHYQELM
                     ERYRSLFGAESATE"
BASE COUNT          646 a         1237 c         1267 g          648 t
ORIGIN      
        1 ctgctcatcg tcggagtttt ctacggagcg cgggtcttcc gcaaggaatc tgcctgagaa
       61 ttccggtatc tgccgcgccc tgctcggccg ataccatcac atcgtggcga gaagcatcgg
      121 gatactgacc gccggcggcg acagccccgg tctgaatgcc gccatcaggg cggtgggcaa
      181 ggccgcccag cgcgcctacg ggatgcacgt catcggcttc tatgacgggt ttctgggatt
      241 gatgcagaac cgtttcgtcc ggttcgactc cgattccctc tccggcatcc tgaccagggg
      301 agggacagtc ctcgggacca gccgagccaa ggtcgacaag atggagatgg gggacggcag
      361 gcgcatggac atgcggtcgc tgatgcgtga cgtcgtcgat cagcacgatc tagaagctct
      421 cgtggtcctc ggcggcgacg gcacgatgcg caatgccctc aagctcgagg acgtcggcat
      481 caacgtgctc gcgcttccca agacgatcga caacgacgtc accggcaccg acgtcacgtt
      541 tggattcgac accgcgatgg gcatcgccac cgaggcaata gaccggctgc actccaccgc
      601 acactcccat catcggatca tcctcgccga ggtgatggga catgaggtcg gatggctggc
      661 catgggggcc gggctcgccg gcggcgccga tgtgatcctc atccccgaga tcccatatga
      721 catcgaggcc attgccacgt cgatccaagc ccgcaaggct gcaggcagcg ccttttccat
      781 aatcgccgct gccgagggtg cctattccag agaggacgct cgtctccggg cctcgctgcg
      841 agccgaaatc gagggcacgg aaggtgaaga gcggcgagtg gcgaaggaaa ggctgacagc
      901 cctcgccagg tcccctcgga ccgagaaact ggctgccgcc ctcgaggatg ccaccgggct
      961 ggaatcgagg gtcaccatcc tcggccatgt gcaacgcggc ggcacgccct cccccgccga
     1021 tcgtttgctt gccaccaggc tgggcacggc caccgccgcc tacatcgccg atggggtgca
     1081 cggggtgctg gtggcagcca gaggccagtc gaccgagccg gtgcccctca aggagattgc
     1141 cggagtcacc aagttcgtgc cactcgatca tccctgggtc cactccgccc gaagtctggg
     1201 catcgttctc ggtgactgat tcaacgctct taggtgctgt ccttgccggg ggagcgagcc
     1261 ggcgaatggg caccgataag gccgacgtcg aggtctcggg gagcaccctc ctcgtccggg
     1321 ttgcgtcggc tttggcggaa gtcgccgacc gcgtcgtggt gctcggcggg gaacgcgagg
     1381 gctacgagtc ctggccggac cgtgccccgg gctcggggcc actggccggc ctggccacag
     1441 ccctgagccg catgaccgag gatcgggccc tcgtggtggc ggtcgacaac gccttcgtcc
     1501 agccagggac cctggcccgt ttggcgtcgg tcgagtcgga cctccccgtc gtcccggtcg
     1561 atcacgccgg ggtgcggcag gtcacttgtg ccgtgtaccc acgccagatc gccggccagg
     1621 cagtcgagga ggcagagtct ggcggatccg tgcagacgtt gctggaccgt gtctcctttc
     1681 tcccggtcac gcccgaccaa tgggaaacct ggggcgagga cggccggagt tggtactcgg
     1741 cggacacgcc cgaggctctc gaaacggggt tgcgccgctt cacttgaaac cggtcagagg
     1801 gcggcacgct cctcgaggaa acgtcgttcg gtttcgttgg tggtgaggga gagggcacga
     1861 tcgagctccg cgcgagcctc tgcctcgtcg cccagctcgc gcagcatctc gctgcgggcg
     1921 agatgaaagc cgagataacc gtcgagatct cgcgccagat cacccaatgc ctcgagccct
     1981 tcccgtgcgc catgggcctc gaagacggcg accgcgcggt tgagccggac caccggcgcg
     2041 gggtgaatcg cggctagacg gtcgtacagc tcgacgatcc gagctcggtc ggtcgattca
     2101 ggcgaggagg cgatggcgtg ctgggccgcg atctcggcct gcagccgata ggggccggaa
     2161 ggaccgagga ggcgggcgcg ttcgagaagg tcgagcccct gggcgatcgc tccggcgtcc
     2221 caacgagcgc gatcctgatc ccggaggagg accaatcgac cggaatcgtc gacccgggca
     2281 tcgcgccggc tgtgctgaag caacatcagt gattggagcc ccaggacctc tgcttcctcc
     2341 ggcatcagtt gtgacaagac cgctcccagc ccgagggctg actcggcgag ttcggtccgc
     2401 accaggtccc cgccggtggt ggcgaaatag ccctcgttga acaccaggta gacgacggcg
     2461 agcaccgcgg agagcctgtc gcccaggtcc gccccggtcg ggacctgaaa cgggatcccg
     2521 gcgtcacgga tcttcgcctt ggccctcacc agacgttggg ccatcgtggg ctcaggcacc
     2581 aggaaggcgt gggcgatctc gaacgtggtg agcccgccca ctgtgcgcaa cgtcagagcg
     2641 acctgcttgt ccgtcgacaa cgaagggtga cagcacgcga agagcatctg aagacgatcg
     2701 tcaccgagct cgccactgct caccacttct ggagggttgt catggtctgc cttctcgtaa
     2761 ccggccagga tcgacgcctt ccgcatgagg gtggcccgac gacgaatccg gtcgatgccc
     2821 cgacgttggg ccacagccga tatccatccg ggcgggttgt cggggatgcc ttgctcgggc
     2881 caggtgcgca acgcctcgac catggcgtcc tggaccgctt cctcggcgag gtcgatgtcc
     2941 cggaatcggg tggctagccc ggagaggacc accgcataag actccctgaa cgtcgcctcg
     3001 atgatggtct tgtggtcgcg cgggctcatc cccaccaagt cttgtcgcga tcatcaccgt
     3061 ggatgtcgaa cgggccgggc tcccgtcgac cgatgcggat cagcgtcacc tctatcctcg
     3121 gctgccgtgc cagtcgattt gccctctccg acgatcgatc cgggggcgtt cgtagcccct
     3181 ggcgctcatc tgtatgggga tgtgacgatc tcgaacgggg tgttcgtcct cttcggcgtg
     3241 gtgatccggg ccgagctcga tcgagtcgag gtcggggcct acaccaacat ccaggacaac
     3301 tcggtcctcc actgcgacga gcaaatccca tgcgtgctcg gggagcgggt gaccgtgggt
     3361 catgctgccg ttgtacatgg tgccctcgtc ggcgaccact gtctcatcgg catcggcgcc
     3421 aagttgctca accgctcgcg actcggcgaa ggcgcctggc tcgcttcggg atcagtactt
     3481 ccggagggca aggagattcc gccgtggact ctggggatgg gcatcccggc aaggccagtg
     3541 cgggagctga ccgacgccga ggtcgagagg gcgaacgatg gagtggacca ctaccaggag
     3601 ctgatggaga gataccggtc cctcttcgga gccgaatcgg cgactgaata ggtcgaccga
     3661 ccgccgcgcc ctacgatggg ctcgtggcca aggaggttgc cgtcctgctc accgaaggcc
     3721 cccgcgaagc gtgcggggtc ttcgccgcct acacaccccg tcaacgagtc tccagcttga
     3781 tctacttcgg tctctacg
//
LOCUS       DASTXO010000251         3178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1305250, whole genome shotgun sequence.
ACCESSION   DASTXO010000251 DASTXO010000000
VERSION     DASTXO010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305250"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VFU96_05355"
     CDS             complement(<1..339)
                     /locus_tag="VFU96_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEU5112723.1"
                     /translation="MTDAELPLVGVGVALVEDDRILLVQRGHEPSKGHWAVPGGKVNH
                     GETLRDAAIREVAEETGLTVEIDDVIWVGEHISEHGHIVLIDFLGSVVGGELTAGDDA
                     DRVEWVPLGVA"
     gene            complement(326..802)
                     /locus_tag="VFU96_05360"
     CDS             complement(326..802)
                     /locus_tag="VFU96_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5112724.1"
                     /translation="MNVTAREAGADDVPSLVRLYGGLAEEMTALHPMWPLADGLPEPI
                     DASFQRLLDDPDGYVVIGELDGYPFGFATALVEDILPQANGEQVGSIRLIYVEPGARE
                     VALGEEMREFVMELLRGRGVSKFDAHVLPGHRLAKNFFEAGGFAARSIVMHHDDGR"
     gene            complement(799..1533)
                     /locus_tag="VFU96_05365"
     CDS             complement(799..1533)
                     /locus_tag="VFU96_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012903538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEU5112725.1"
                     /translation="MPSDRVTVVIPTYNERENLPDISKAVIVHGYELLVVDDSSPDGT
                     GDLADRLANEDSRISVLHRSSKEGLGPAYAAGFDRALDDGADIVIEMDADFSHDPADL
                     PRLVHAIGDGADLAIGSRYVSGGSTPDWPLTRRVISKGGNLYARMMLGIPIRDATAGF
                     RAFRSEALRSLPYREAQSSGYAFQVEMAMRAHEGGLRVVEVPISFRDRTRGTSKMGTG
                     IVVEAMRLVTVWGWKRAVRGVRSWGR"
     gene            complement(1523..1993)
                     /locus_tag="VFU96_05370"
     CDS             complement(1523..1993)
                     /locus_tag="VFU96_05370"
                     /inference="COORDINATES: protein motif:HMM:NF025204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 family protein"
                     /protein_id="HEU5112726.1"
                     /translation="MAKEEVEQLTEDLEELEDAPEDELDDEAIPELDEELEDDVDLEP
                     DFEDEAFEEEESEEAEYDSEEEEGEEEHDEETTEALEELESEELQLLDEEAGEALLVD
                     EAEELRAIRREELTMNVDAQLQRSDEFVCQSCFMVKRTSQLANRKKMLCVDCAF"
     gene            complement(2049..2933)
                     /locus_tag="VFU96_05375"
     CDS             complement(2049..2933)
                     /locus_tag="VFU96_05375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HEU5112727.1"
                     /translation="MPKWQVYVNRAAGRKAVEPAMIGDLLRGLGLDFELHVPDSSDET
                     THAIREAARSGATHFAVAGGDGTVNLAVNALMPLGLAVPPVIGVLPVGTGCDLLRTFG
                     MPQDVAGAARHLTSDDVYDIDVGTLEGSWGLRYFVNVAQTGVGAAAAQTAMALGRRLG
                     AARYPLAFVARLPGFPRARVKLTTERRTIESEALAVILANAQFFAGGWNVAPKAMLVD
                     GALDVQIIDCAKTSAPALVPKVIKGTHLADPAVRRLSAAEFSIETDPPWPVECDGDLV
                     GNTPVRGRVIPAAISLKI"
     gene            complement(2934..>3178)
                     /locus_tag="VFU96_05380"
     CDS             complement(2934..>3178)
                     /locus_tag="VFU96_05380"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA helicase"
                     /protein_id="HEU5112728.1"
                     /translation="LSPTRRPDPGFGIPAHEWASGTDFDDLSNKGMAPGDFVRVSRQL
                     VDLVRQLRDSAPGLGDDARAALRAIDRGGVAAQGVG"
BASE COUNT          566 a         1116 c          929 g          567 t
ORIGIN      
        1 ggccacaccg agaggaaccc actccacccg gtcggcgtcg tcgcccgcag tcagctcacc
       61 gccaaccaca gaaccgagga agtcgatcag gacgatgtgg ccatgttcgg agatgtgctc
      121 gccaacccag atcacatcat cgatctccac ggtgagaccc gtctcctcgg cgacctcgcg
      181 aatcgcagcg tccctgagtg tctcgccgtg gttcaccttt ccgccgggca cggcccagtg
      241 ccccttcgaa ggctcgtgac ctcgctgcac caggagtatg cgatcgtcct cgaccagggc
      301 cacaccgacc ccaacgagcg gaagctcagc gtccgtcatc gtggtgcatg acgatcgagc
      361 gagccgcgaa gccccccgct tcgaagaagt tcttggcgag gcgatggccg gggagcacat
      421 gtgcatcgaa tttcgagacc ccgcgcccgc gcaggagctc catcacgaac tctcgcatct
      481 cctcccccaa agcgacttcc ctcgccccgg gctcgacata gatgaggcgt atcgagccga
      541 cttgctcacc gttggcctgg ggcaggatgt cctcgaccag ggccgtggcg aacccgaagg
      601 gataaccgtc caactccccg atcaccacgt aaccgtcggg atcgtcgagg agacgttgga
      661 acgaggcgtc gatgggctcc gggaggccat ccgccaacgg ccacatcgga tgaagtgcag
      721 tcatctcctc tgcgagaccc ccgtacagcc ggaccagcga agggacatca tcggcgcccg
      781 cctccctggc cgtgacgttc accgccccca cgagcgcacc ccgcgaacgg ctcgcttcca
      841 cccccatacc gtcaccaacc tcatggcttc taccacgatc ccggtcccca tcttcgacgt
      901 gcctctggtc cgatctcgaa agctgatcgg gacttccacg actctcaaac cgccctcatg
      961 ggcccgcatc gccatctcga cctggaaggc atagcctgac gactgcgcct cgcggtacgg
     1021 gaggctccgc agtgcctcag accgaaaggc gcggaaccca gccgtcgcgt cacggatagg
     1081 gattccgagc atcatccggg catagagatt gccccccttc gagatgacac gtcgcgtcag
     1141 tggccagtcc ggagtgctac cccccgagac atatcgggag ccgatggcga gatccgcccc
     1201 atctccaatg gcatgcacca gacgaggaag gtcggcgggg tcgtgggaga agtccgcatc
     1261 catttcgatg acgatgtctg cgccatcgtc cagcgcccgg tcgaagcctg ccgcataggc
     1321 cgggcccagc ccctccttgc tcgagcgatg gagcacggag atccgggagt cttcgttagc
     1381 gaggcggtcg gcaagatcac ccgtgccatc cggggacgag tcgtcgacga cgagcagctc
     1441 gtagccgtgc acgatgactg ccttcgagat gtcggggaga ttctcgcgct cgttgtacgt
     1501 gggaatgacg accgttaccc ggtcagaagg cacagtccac gcacaacatc ttcttgcgat
     1561 tcgccagctg gctcgtccgc ttcaccatga aacaagactg gcacacgaac tcatccgatc
     1621 tctggagctg ggcatcgacg ttcatggtca gctcctcacg acggatggcg cgtagctcct
     1681 cggcctcgtc gaccagaagg gcctcgccgg cctcttcatc gagcagctgc agctcctcgc
     1741 tctcgagctc ttcgagggcc tcggtcgtct cctcgtcgtg ctcctcttcg ccttcttcct
     1801 cttcggagtc gtattcggct tcctccgact cctcctcttc gaaagcctca tcttcgaagt
     1861 cgggctcgag atcgacatcg tcctcgagtt cctcgtcgag ctcaggtatc gcctcgtcgt
     1921 cgagttcgtc ttcgggcgcg tcctcgagtt cctctaagtc ttcggtgagc tgttccactt
     1981 cttctttggc catcgatcca ctcggctttc gggccccttg gcggtgcggg agtataagtc
     2041 gccttacctc agatcttgag actgatcgcg gccgggataa cacggcctct gaccggtgta
     2101 ttccccacga gatccccatc acattcgacg ggccagggtg ggtccgtctc gatgctgaac
     2161 tcggctgccg agagccgccg cactgcagga tcggcgagat gagtgccctt gatcaccttc
     2221 ggcaccaacg cgggcgcgct cgtcttggca cagtcgatga tctgcacgtc gagggcccca
     2281 tcgacgagca tcgccttggg cgcaacgttc cagccaccgg cgaagaactg tgcattggcc
     2341 agtatcaccg ccaaggcctc tgattcgatc gtgcgccgtt ccgtggtgag cttcacccgc
     2401 gccctgggga atccgggaag gcgcgcgacg aacgcaagcg gataacgggc cgccccgaga
     2461 cgacggccta gcgccatggc tgtctgcgcg gcggccgcac caacacctgt ctgggcaacg
     2521 ttgacgaaat agcgaagccc ccagctcccc tccaaagtcc ccacgtctat gtcgtatacg
     2581 tcgtcgctgg tcagatgacg cgcagccccg gccacgtcct gcggcatgcc gaaggtgcgc
     2641 agcagatcgc accctgtgcc tacgggcaag acgccgatca ccggtgggac cgcgagcccg
     2701 agtggcatca gtgcgttcac cgccagattg accgtgccgt ccccgcccgc cacggcgaag
     2761 tgagtggcac cggaccgcgc tgcctcccga atcgcgtgcg tcgtctcatc cgacgagtcg
     2821 gggacgtgga gctcgaaatc gaggccgagc cctcgtagca ggtcgccgat catcgcaggc
     2881 tccactgcct tccgacccgc tgctcgattc acatagacct gccacttggg caactagccc
     2941 actccctggg cggcgacccc ccctcgatcg atggccctga gcgcggccct ggcatcgtcc
     3001 cctagacccg gggcgctgtc gcgcagctga cgcacgagat ccacgagttg acgtgagaca
     3061 cgaacgaagt cgccgggcgc catccctttg ttactcaggt cgtcgaagtc ggtgcccgac
     3121 gcccactcgt gagccgggat gccgaagcca gggtcgggcc gccgggttgg gctgagcc
//
LOCUS       DASTXO010000252         4565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_52450, whole genome shotgun sequence.
ACCESSION   DASTXO010000252 DASTXO010000000
VERSION     DASTXO010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52450"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VFU96_05385"
     CDS             <1..451
                     /locus_tag="VFU96_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112729.1"
                     /translation="EQSDGRSRAWVDTEGGPKALHVKGLVSDRFALTDPPEPRYEPNP
                     DFGVNQEDQTQTELGGWSVTVTRRILLGGDENQVVEEQEWTVRYAPRFAVYEVHPCMV
                     PGTSTPCPTTTTAPPTTVAPTTQPPATTAPPPTTTTTTAPPTPPTTP"
     gene            complement(458..751)
                     /locus_tag="VFU96_05390"
     CDS             complement(458..751)
                     /locus_tag="VFU96_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112730.1"
                     /translation="MGQTVIVNQVQQLDDVLLLDTDRSFTGQDGSAMSPSVRGHGVPG
                     VLAGELFDLGLGIDHVYVLQNTVTVRRPGGWDEETKAAVTTVVERFLLFYDES"
     gene            complement(769..2202)
                     /gene="pyk"
                     /locus_tag="VFU96_05395"
     CDS             complement(769..2202)
                     /gene="pyk"
                     /locus_tag="VFU96_05395"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU5112731.1"
                     /translation="MQRHTKIIATLGPAVASAARIHALVEAGIDVARLNFSHGDRELH
                     RTMAGWVREASRETGRTVALMQDIQGPKLRVGEFANGRVTLEPGRHVTLVANGTLGSE
                     SVIPVGYEPLLEDVEPGDRIQLADGLIRCEFVDRTEAGLVAAVRVGGVLSDHKGVAVP
                     DSKLSLEIVTPKDESDLAFGRELGVDYVAASFVRSGDDVRSVAQLAGDVPIVAKVELA
                     QAYENLDDILGPSSGVMVARGDLGVQLPLERIPLIQDEILKKTNAAGRISITATEMLE
                     SMTKSPRPTRAEVTDVAHAVMAGTDAVMLSGETAVGDYPVETVTAMASICVAVEEGTL
                     SIRGHHPIPFVGDGNTVASAVSQAATEIAVNVDAQTIVAFTESGNTARLISKYRPEAR
                     IVAFTPNEATLHQMALYWGVSPHPFERRTYTDQELASAADILEREGVVSKGDRVVMVA
                     GVPPNVQASTNLVKVHVIGEVPGGLGS"
     gene            complement(2231..3679)
                     /gene="gatB"
                     /locus_tag="VFU96_05400"
     CDS             complement(2231..3679)
                     /gene="gatB"
                     /locus_tag="VFU96_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEU5112732.1"
                     /translation="MSWEPVIGIEVHVELMTASKMFCGCAVSFGGEPNTRVCPTCLGL
                     PGALPVTNRVAIESIMAIGLALDCQISERSVFHRKNYFYADLPKNYQISQFDVPVCHD
                     GYLDIDVDGQTRRIGIERAHMEEDTGKSTHIGAGGRIHAAEGTLLDFNRSGVPLVEVV
                     SRPDLRSAAESRVYAQELRSIVAELGVSDARLEEGSIRFDANVSIRPDGEETFGTKVE
                     IKNMNSFRSLERAIAFEIERQIELVESGGEVVQETRHWDEETGITHSMRVKEGSSDYR
                     YFTEPDLVPMVMTPEWVAGIEKRLPELPAGRRARYAAQGLSIEMAGVLSALDSDLRRL
                     YDDALLSGAPPRSAANWITGEVLGWVRKAGVDAREARLDGPGLAELIEMVDDRLVSSS
                     AAKDVLAGVLSGEGRPREVAEKRDLLQLSDTGALEMAVAAVLEANPGAVDEFRGGEEK
                     VVGFLVGQVMKATEGKADPRMVNQLLRERMSG"
     gene            complement(3676..>4565)
                     /locus_tag="VFU96_05405"
     CDS             complement(3676..>4565)
                     /locus_tag="VFU96_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019127203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEU5112733.1"
                     /translation="YGTVSRYGLIAFASSLDQIGPFTKTVEDSAAALAVIWGYDPLDS
                     TSYQGEYPNPSARLDRGVEGLRIGVVTELSGEGFEPAVEAATTDMVARLEKEGVEVME
                     ISLPTFDIALSAYYLIAPAEASSNLARFDGIRYGLRVDGATTEELMARTRADGFGPEV
                     IRRVLLGTYSLSAGYYDAFYGQAQRVRAAIQEDFARAYEQVDVLVSPTSPTVAFDAGA
                     RTSDPLAMYLSDICNIPANMAGHPAISVPVGLDEAGLPIGFQVMAPALGEETMYQVAA
                     EVERLAGFEISPRGLGGAA"
BASE COUNT          801 a         1583 c         1415 g          766 t
ORIGIN      
        1 ggagcagtcc gacggtcgga gccgggcctg ggtggacacc gagggaggcc ccaaggcctt
       61 gcacgtcaag ggtttggtct ccgaccggtt cgcgctcacc gacccaccag agccccgtta
      121 tgagccgaac cccgatttcg gcgtcaacca agaggaccag acccagaccg agctcggcgg
      181 ctggagcgtg accgtgaccc ggcgcatcct gctcggcggc gacgagaacc aggtggtcga
      241 ggagcaagag tggaccgtgc ggtacgcccc gagattcgcc gtctacgagg tgcacccctg
      301 catggtgccg ggcacgtcca cgccctgccc gaccacgacg acggccccac cgaccacagt
      361 ggccccgacc acgcagccgc cggcgactac cgcaccgccg ccgactacga ccacgaccac
      421 ggcccctcca actcccccaa cgactccctg atcccggtca ggactcgtcg tagaagagca
      481 ggaaacgctc gaccaccgtg gtcaccgcag ccttggtctc ttcatcccaa ccacctgggc
      541 gcctcaccgt gacggtgttc tggagcacgt agacgtgatc gatgccgagc ccgagatcga
      601 agagctcacc ggcgaggacg ccgggaacgc catgaccgcg aacggaaggc gacatcgcgc
      661 tcccgtcctg accggtgaag ctgcgatctg tgtcgaggag caatacatca tcgagctgct
      721 gcacctggtt cacgatgacg gtctgtccca tgagcgtcgg cagggtactc aggaccccag
      781 gccgccgggg acctcgccga tcacatgaac cttgaccaga ttggtggaag cctgcacgtt
      841 ggggggaact ccggccacca tgaccacccg atcgcccttg ctgaccactc cttctcgctc
      901 caggatgtcc gccgccgagg caagctcctg gtcggtatag gtgcggcgct cgaaggggtg
      961 agggctgacc ccccagtaga gggccatctg gtgaagtgtc gcctcgttgg gggtgaaggc
     1021 gacgatccga gcctcgggcc ggtacttgga gatcaaccgg gcggtgtttc ccgattcggt
     1081 gaaggccacg atcgtctgtg cgtcgacatt caccgcgatc tcggtggccg cctgcgacac
     1141 ggccgaagcc acggtgttgc cgtcgccgac gaagggtatg gggtgatggc cgcggatcga
     1201 aagggtgccc tcctccaccg ccacgcagat ggacgccatc gccgtgaccg tctccactgg
     1261 atagtcgccg acggcggtct cgcccgacag catcaccgcg tcggtgccgg ccatcaccgc
     1321 gtgggcgacg tcggtgacct cggcccgagt gggccggggg ctcttggtca tcgactcgag
     1381 catctcggtc gccgtgattg agattcggcc ggccgcattc gtcttcttga ggatctcgtc
     1441 ctggatcaga gggattcgct ccagcggcag ttgcacgccg aggtcacccc gggccaccat
     1501 gaccccggag gagggcccga ggatgtcgtc caggttctcg taggcctggg cgagctcaac
     1561 cttggccacg attgggacat cgccggccag ctgggccaca ctccggacgt cgtctccgct
     1621 ccgcacgaac gacgccgcga cgtagtcgac cccgagctcc cggccgaagg caaggtcgga
     1681 ctcgtccttg ggagtgacga tctccaggct cagcttgctg tcggggacgg ccacaccttt
     1741 gtggtcggac aggacccctc ccacccggac cgcggccacc aggccggcct cggtccgatc
     1801 cacaaactcg catcgaatca acccgtcggc aagctgaatc cgatcgcccg gctccacgtc
     1861 ctcgagcaac ggctcgtaac cgaccgggat cacgctctcg cttcccaatg tcccgtttgc
     1921 cacgagggtg acatgacggc cgggctccag cgtcaccctg ccattggcga actcaccaac
     1981 ccgcagcttt gggccttgta tgtcctgcat gagcgccaca gttcgccccg tctcgcggga
     2041 ggcctcccgc acccagccgg ccatggtccg atgtaactcg cgatcaccat gcgagaagtt
     2101 gagccgtgcc acatcgatgc cggcctccac cagggcgtgg atcctggcgg ccgaagccac
     2161 ggcgggcccc agcgtggcga tgatcttggt gtgccgctgc aatgtgatac cgacggtaac
     2221 cgttgccggg tcagcccgac atcctctccc gtaggagctg attgaccatc cgggggtcgg
     2281 ccttgccctc ggtcgccttc atgacctggc cgaccaggaa tcccaccacc ttctcctcgc
     2341 cgcctcgaaa ctcgtcgacc gctccggggt tagcctccag gaccgcggcc accgccatct
     2401 ccagggcgcc ggtatcactc agctggagga gatcccgctt ctcggcgacc tcccggggcc
     2461 ggccctcacc ggagagaaca cccgcgagca cgtcttttgc cgcggagctc gacaccaagc
     2521 ggtcatcgac catttcgatc agctcggcaa ggccaggccc gtcgagcctg gcctctcgag
     2581 cgtcgactcc cgctttgcgc acccacccca gcacctcacc ggtgatccag ttggcggcgg
     2641 atcggggagg ggcaccggag agaagcgcgt cgtcgtagag ccggcgcagg tcggagtcga
     2701 gagcggagag gacaccggcc atctcgatcg acagtccctg tgctgcatat cgagcccgac
     2761 gaccagcggg caactcgggg aggcgcttct cgatgccggc tacccactcc ggcgtcatca
     2821 ccatgggcac gaggtcgggc tcggtgaaat agcgatagtc cgacgagcct tctttgaccc
     2881 gcatggaatg agtgatgccc gtctcctcat cccagtgacg tgtctcctgg acgacctcgc
     2941 ccccggactc gacgagttcg atctgccgtt cgatctcaaa cgcgatcgcc cgctccaacg
     3001 agcgaaatga gttcatgttc ttgatctcga ccttggtgcc gaaggtctcc tccccgtcgg
     3061 gtcggatcga cacgttggcg tcgaatctga tcgatccctc ctcgagtctc gcgtccgaca
     3121 cccccagctc cgccacgatc gatcgcagct cttgggcata cacccgtgat tcggcggcgc
     3181 tgcgcaggtc aggtctggac accacctcga ccagtggcac tccggatcgg ttgaaatcga
     3241 gcagggtgcc ctcggcggcg tggatacggc ctccagcccc gatatgggtc gactttcccg
     3301 tgtcctcttc catatgggcg cgctcgatgc cgatgcgccg ggtctggccg tcgacgtcga
     3361 tgtcgagata cccgtcatgg cataccggga catcgaactg ggagatctgg tagttcttgg
     3421 gcaggtcggc atagaaatag ttcttccggt ggaacaccga tcgttccgag atctggcaat
     3481 ccagggccag cccgatcgcc atgatcgact cgatcgcgac ccgattcgtc accgggaggg
     3541 cgccagggag acccagacac gtggggcaga ccctggtgtt cggctcgcct ccgaaggaaa
     3601 cagcgcagcc acagaacatc ttcgaggccg tcatcagctc gacgtggacc tctatcccga
     3661 tcaccggctc ccagctcacg ccgccccccc caatccccgc gggctgatct cgaagccggc
     3721 cagacgctcc acctcggccg caacctggta catggtctcc tcaccgagtg cgggtgccat
     3781 cacctggaac ccgatgggca ggcctgcctc gtcgaggccg acggggacag agatggcggg
     3841 gtggcccgcc atgttggccg ggatgttgca gatgtccgac aggtacatgg cgagcgggtc
     3901 ggaggtacgg gcaccggcat cgaacgccac ggttgggctc gtcggcgaga ccagcacatc
     3961 gacctgctcg taggccctag caaaatcctc ctgaatggcg gcccggaccc tctgtgcctg
     4021 gccgtagaag gcgtcgtagt acccggcaga aagcgagtag gtgccgagga ggacgcgccg
     4081 aatcacctcg gggccgaatc cgtctgctct cgttcgcgcc atcagttcct cggttgtggc
     4141 gccgtcgacc ctgaggccgt accggatccc atcgaatcgg gccaggttcg aggaggcttc
     4201 ggcgggggcg atcaggtagt aggcagacaa ggcgatgtcg aaggtgggca gcgatatctc
     4261 catcacctcc accccctcct tctccagtcg ggcgaccatg tcggtcgtgg ccgcctcgac
     4321 cgcaggctcg aagccctcgc cggacaactc ggtgaccacg ccgatgcgga gcccctcgac
     4381 ccctctatcc agtcgagcgc ttggattcgg gtactcaccc tgatacgaag tcgagtcgag
     4441 tgggtcatac ccccagatca cggccagggc cgccgccgag tcctccaccg tcttggtgaa
     4501 tgggccgatc tggtcgagcg acgaggcaaa tgcgatcaac ccgtaccggc tcaccgtccc
     4561 atacg
//
LOCUS       DASTXO010000253         2003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_89617, whole genome shotgun sequence.
ACCESSION   DASTXO010000253 DASTXO010000000
VERSION     DASTXO010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89617"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="VFU96_05410"
     CDS             <1..1173
                     /locus_tag="VFU96_05410"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEU5112734.1"
                     /translation="LIQDAVVTGDPVSEELLLQLEVESDNLNQSFFEARSLAEGDDLA
                     VIEEIRTGEASRTALRMVAAITESGMALESINPQRWFEANTMRIEQVDDLIPVVHQRV
                     STVAGAALDRAERALWTGSILLSALFLLTVLVGSNAVFASRERQAVLAEYGQLSDGLR
                     QWFAPTSFPDSESVDIAARYIPASVRTQSGGDWYDVYEVGDSLAVVIGDVAGHGADAT
                     AQMAQVRNVLRGQSTARTLDPAAQIDLLDKTMSDSGIVATMTYGLLEVSTGEFVHTRA
                     GHIPLLVRSSAGDVRIEEEGAGPPVGSDLHLKREIKRTRLYPGDVLILITDGLVEGVN
                     RDIDRALDEIAKTLTEADPSPAAMLDELFGMNEASIDDAAALLITWNPKNAGGPGR"
     gene            complement(1240..>2003)
                     /locus_tag="VFU96_05415"
     CDS             complement(1240..>2003)
                     /locus_tag="VFU96_05415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5112735.1"
                     /translation="ELELVRRQTLSHLSDDELFELVPADRDARDEIFSRYEGWAASVA
                     NRYRRGGAHADDVGQAASIALVNAIDRFDPDFGVKFTTYARKTIIGTLKRYLRDTTWS
                     VRVPRSLSDTTVELNRAVAELSQKLGRTPMPSEMAEELGRPIEEIEDAMEAALAYNAK
                     SVDAPFSEDDETSLLRVLGSEDEHFDLADIWHSLEPLISDLEPQEQEVLFLRFFEDMT
                     QSDIAEVVGVSQMQVSRLIARALDTLRARLTPDSA"
BASE COUNT          379 a          628 c          643 g          353 t
ORIGIN      
        1 ctaatccagg acgccgtcgt caccggcgat cccgtttccg aggagctcct gcttcaactc
       61 gaagtggaat cggacaacct caaccagagc ttcttcgagg cgagatccct ggctgaggga
      121 gatgacctgg ccgtcatcga ggagatcagg acaggagagg cttcccgaac cgccctgcgg
      181 atggtggcag cgatcaccga gtcgggcatg gccctcgagt cgatcaatcc gcagcgatgg
      241 ttcgaggcca acacgatgcg aatcgagcag gtcgacgacc tcatcccggt ggtgcaccag
      301 cgagtatcga ccgtcgctgg tgcagcgctc gaccgcgccg agcgtgcgtt gtggaccggc
      361 tcgatcctgc tcagcgcact cttcctactc actgtgctgg tgggcagcaa cgccgtcttc
      421 gccagtcgag agcgccaggc ggtgctcgcc gaatacggcc agctgagcga tgggctgcgg
      481 cagtggtttg cccccaccag cttccccgat tccgaaagtg tggacatcgc ggcccgctac
      541 ataccggcat cggtcaggac ccaatcgggc ggagactggt acgacgtcta cgaggtcggg
      601 gactcgctgg cagtggtcat tggtgacgtc gcagggcatg gtgccgatgc aacagcacag
      661 atggcacagg tgaggaacgt cttgcgcggc cagtcaactg cgaggacgct cgatccggcg
      721 gcccaaatcg acctactcga caagacgatg tccgactcgg ggatcgtcgc cacgatgacc
      781 tatggccttc tcgaagtctc cacgggagag ttcgtgcaca cccgcgccgg acacatcccc
      841 ttgctggttc ggagctctgc cggagacgtc cgcatcgagg aggagggggc gggtccgccg
      901 gtcgggagcg acctccacct caaacgcgag atcaagcgca cccgcctcta tcccggggac
      961 gtgctcatcc tgatcaccga cgggctggtc gagggtgtga accgcgatat cgatcgagcc
     1021 ctcgatgaga tcgccaagac gctcaccgag gctgacccat caccggcagc gatgctggat
     1081 gagctgttcg ggatgaacga agcgtcgatc gatgatgcgg cggctctgct catcacctgg
     1141 aacccgaaga acgccggcgg ccctggtcgg tgacctgaat cacctcgtcg tcgtggatca
     1201 cgcgctcggt cgacggcgca ctggctcaga gcgaaacgat caagcagaat caggggtcag
     1261 gcgtgccctc aacgtgtcga gggcccgagc gatcagccgc gatacctgca tctgggagac
     1321 accgactact tcggctatgt cggactgggt catgtcttcg aaaaagcgaa gaaagaggac
     1381 ctcttgctct tgaggctcga gatccgagat cagtggctcc agggaatgcc agatgtccgc
     1441 tagatcgaag tgctcgtcct cgctgcccaa cacccggagc aggctggtct cgtcgtcctc
     1501 tgagaacggg gcgtcgaccg acttggcgtt gtaggcgaga gctgcctcca ttgcgtcctc
     1561 gatttcctcg atcggccgcc cgagctcttc ggccatctcc gagggcattg gagttcttcc
     1621 cagcttctgg gacagctcgg ccaccgcccg gttgagctcc accgtggtgt cactgagcga
     1681 ccgggggacg cgaaccgacc aggtggtgtc acggagatag cgcttcaggg tcccgatgat
     1741 cgtcttgcgc gcgtacgtgg tgaatttgac gccaaagtcg ggatcaaatc ggtcgatggc
     1801 gttgaccagg gcaatcgagg ctgcctgtcc gacgtcatcg gcatgggccc cgccccggcg
     1861 ataccggttg gccaccgaag ccgcccagcc ctcgtacctg gagaaaatct cgtcgcgggc
     1921 gtcgcggtcg gccggcacca gctcgaacag ctcgtcgtcc gacagatggg acagggtctg
     1981 acggcgcacc agctcgagtt cga
//
LOCUS       DASTXO010000254         2314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_937054, whole genome shotgun sequence.
ACCESSION   DASTXO010000254 DASTXO010000000
VERSION     DASTXO010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937054"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..788)
                     /locus_tag="VFU96_05420"
     CDS             complement(57..788)
                     /locus_tag="VFU96_05420"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEU5112736.1"
                     /translation="MATDREARKENPPYEPHIGETRQYWRDIILGVNDGLVSIFLLVV
                     GVVGGGLDTDQVLLTALAGALAGAVSMAAGEYLATKSQDQVLEAELKLERVHIRDHRQ
                     QELDQLTEMFGDMGLRQEDVDMVVAAFNRSDDAILNAMKALEFGFVDSERRSPYRAML
                     ASGGLFLVGSLPVVIPFFIFASTNVALAWATVLALTGLFIVGVVKARVARHSWLTSGL
                     ENMVIAGIGGVIAWLIGSAVGTALV"
     gene            complement(827..1984)
                     /locus_tag="VFU96_05425"
     CDS             complement(827..1984)
                     /locus_tag="VFU96_05425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU5112737.1"
                     /translation="MISHDRLEALVEGIIPQSVELRRLLHRHPEPANEEHQTTALIAA
                     SLDENGIPYEDRKPQTGLWLDIGPEPAIGFRADLDALPIYEPEENAPRSQNPGWMHAC
                     GHDAHAAIAFGIAVALRQLQLPVGVRLLFQPAEEAFPGGASNFVDEGLADGLKGIVAF
                     HVDPSLETGRVGSRVGPITASADQFTIVLDGPGGHTARPHQTVDLIADAARLVHELPG
                     VLRRTVDARSTLTVAFGSIQGGRTSNVIPTRVELQGTARTLDRSLWEVLPGLMDKAIG
                     SLMAWSGAGYTLDYVQAIPPVVNDHAVVQTATAGITQLLGPETVVPTEPSMGGEDFAN
                     YLSVAPGALLRLGSSGKGHDLHSPGFVLDEATVGFGVRAGLGAVIGMAEGL"
     gene            complement(2009..>2314)
                     /locus_tag="VFU96_05430"
     CDS             complement(2009..>2314)
                     /locus_tag="VFU96_05430"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamine-synthetase
                     adenylyltransferase/deadenyltransferase"
                     /protein_id="HEU5112738.1"
                     /translation="GGAIPELRVPGTLEALQGLIGHGLLEAEEERALSESYLFCTRAR
                     LRLHLQMGRAVDSLPTAPAELGSLAASLGFDRAAELRDEYRRVTRRARRVFETRFYE"
BASE COUNT          414 a          771 c          755 g          374 t
ORIGIN      
        1 tgcaagctta cagcaagctg ctaactattg caagcatgtg ctagcggagg gcgggctcag
       61 accagggcgg tgccgaccgc actcccgatc agccaggcga tcacgccgcc aatcccggcg
      121 atgaccatgt tctccaaacc cgaggtgagc cagctgtgcc gggccactct tgccttgacc
      181 accccgacga tgaagagccc ggttagcgcg aggaccgtgg cccacgccag tgcgacgttc
      241 gtggaggcga agatgaagaa cggaatcacc accgggagag acccgaccag gaacagcccg
      301 ccggacgcga gcatggctcg atatggcgac cgacgctccg agtcgacgaa gccgaactcg
      361 agcgccttca tcgcattgag gatggcgtcg tcggagcggt tgaaggcagc caccaccatg
      421 tcgacatcct cctgacgcag acccatgtcg ccgaacatct cggtcagctg gtcgagctct
      481 tgttggcggt ggtcccgaat gtggactctc tccagcttca actccgcttc cagcacttga
      541 tcttgggact tcgtggccag gtactcgccg gccgccatcg agaccgctcc ggccaaggcg
      601 ccggcgagag cggtgagaag gacttggtcg gtgtcgaggc ctccgccgac caccccgacc
      661 acgagcagga agatcgagac gagcccgtcg ttgactccga ggatgatgtc ccgccagtac
      721 tggcgggtct cgccgatgtg gggctcgtac ggagggttct ccttgcgtgc ctctcggtcg
      781 gtcgccacag acgcgatggt acaaccgccg ccgagcccgc tcccggtcag agaccctcgg
      841 ccatgccgat caccgcaccc agaccggccc tcaccccgaa tcccaccgtc gcctcgtcga
      901 gcacgaaccc gggggagtgg agatcatggc ctttccccga tgagccgagc cggagcaacg
      961 cccccggtgc caccgacagg taattggcga aatcctcgcc tcccatcgac ggctcggtgg
     1021 gaaccaccgt ctccggcccg agcagctgag tgatcccagc tgttgcggtc tggacgacgg
     1081 catgatcgtt gacgacgggc ggtatcgcct gcacgtagtc cagcgtgtag ccggcgccgg
     1141 accaggccat caggctgcca atggccttgt ccatgagccc ggggagcact tcccacagac
     1201 ttcgatccag ggtgcgagcc gtaccttgca gctcgacacg ggttggaatc acattcgagg
     1261 tccgtccccc ctggatggag ccgaaggcaa cggtcaatgt gctcctggcg tcgacggtgc
     1321 gcctcagcac tccgggcaac tcgtgcacga ggcgggctgc gtcggcaatg aggtcgaccg
     1381 tctgatgggg ccgggcggta tggcccccag gcccatcgag cacgatcgtg aactggtcgg
     1441 cgctggcggt gatcgggccg accctgctcc ccacccggcc tgtctccaac gacgggtcca
     1501 catggaaggc cacgatccct ttcaaaccgt ccgccagccc ttcgtcgaca aaattcgagg
     1561 cgcctcccgg gaacgcctcc tcggccggct ggaacagcaa cctcaccccg accggcaatt
     1621 ggagctggcg cagagcgacc gcgatgccaa aggcgatcgc ggcgtgtgcg tcgtgaccac
     1681 aggcatgcat ccagcccggg ttctgggaac gaggggcatt ctcctccggc tcgtagatgg
     1741 gcagtgcgtc gaggtccgcc ctgaaaccga tcgccggctc gggcccgatg tccagccaga
     1801 gaccggtctg gggtttgcga tcctcgtagg gaatgccgtt ctcatcgagg gaggcggcga
     1861 tcagcgccgt ggtctggtgc tcttcgttgg ccggctcagg atggcgatgg aggaggcggc
     1921 gaagctcgac cgattggggg atgattccct ctaccagcgc ctcgagccga tcgtggctga
     1981 tcacgtgttg aggctaacca ggtggcgatc actcgtagaa gcgggtctcg aagacgcggc
     2041 gggctcttct cgtcacccgg cggtactcgt ccctgagctc ggcggcccgg tcgaacccga
     2101 gcgacgcggc gaggctgccc agctctgccg gagcggtggg aagcgagtcg acggctcgac
     2161 ccatctgcag gtgaaggcgg agacgcgccc gcgtgcagaa gaggtaggac tcagacagtg
     2221 ctcgctcttc ctcggcctcg agtaatccgt gcccaatcaa cccctgcaag gcctcgaggg
     2281 tgccggggac ccggagctcg ggaatggcgc cgcc
//
LOCUS       DASTXO010000255         4066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1673787, whole genome shotgun sequence.
ACCESSION   DASTXO010000255 DASTXO010000000
VERSION     DASTXO010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1673787"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VFU96_05435"
     CDS             complement(<1..377)
                     /locus_tag="VFU96_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEU5112739.1"
                     /translation="MRAAFTGQVEPETAREDVEKTIAGLDRGELRVAEKVGGDWMVNE
                     WVREAILLYFRLREMETLEVGPFEYHDKIPLKTGLAGQGVRVVPPGTVRYGAFCEPGV
                     IVMPGFVNIGAYVGSGTMVDTWAT"
     gene            460..849
                     /locus_tag="VFU96_05440"
     CDS             460..849
                     /locus_tag="VFU96_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112740.1"
                     /translation="MPYRLLYIGLGLLAVAAIALGVAFNRSGDELVLPEEIEAISPSP
                     GDLVPPQTSVEVDLPVAYRADIYVDDWLVTDVVFVEGTGVYRWSPSPNSSTITEWAPG
                     EHTVRVVWDTISGLPDPGEYEWSFRVG"
     gene            complement(881..1654)
                     /locus_tag="VFU96_05445"
     CDS             complement(881..1654)
                     /locus_tag="VFU96_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5112741.1"
                     /translation="MRTLTDTWTMAERSLRHTTRSMDTIITVVLMPIALMLLFVYVFG
                     GALGAQTSVDYIDFLTPGVVIMTVISGIAYAAFRLATDMQKGIISRFRTMPVRPSSVL
                     SGQALSSTVSNALSCVLVIVVAFLIGFRPVPNAGAWLLAAGLVLLFILATTWLAMFFG
                     LLAKSVEGAGAFSYILFLLIFISPSFVPTASMAPWLQSFAENQPLTPIVDTIRSLLTD
                     GTPGPRFWVALAWAVGILVVSYTLALVAYRRSDPPQAVA"
     gene            complement(1638..2609)
                     /locus_tag="VFU96_05450"
     CDS             complement(1638..2609)
                     /locus_tag="VFU96_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020491533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5112742.1"
                     /translation="MKEPMRAMHEINAIEVRNLEKAYKDVPVLHDVSFTVKQGTVFAL
                     LGANGSGKTTTINILTTLIKADGGTASVAGFDVATQPASVREVISVTGQSAAVDYILT
                     ARENLMLMAELRHVDDPEQVASSLLDKFDLNEAADRRLLTFSGGMRRRLDIAMSLVGE
                     APIVFFDEPTTGLDPQSRNDMWLTIRELAEKGTTIFLTTQYLEEADRLADQIAILHGG
                     RVVAGGTPEELKTMVPSGLVELDFAEDNELAVAQRALTSGHELSRVDSKLVIASDGSV
                     ADMADMFIRLNNAGVEPTRFIAKEPTLDDVFFQILGEDKEETHANSH"
     gene            complement(2634..3488)
                     /locus_tag="VFU96_05455"
     CDS             complement(2634..3488)
                     /locus_tag="VFU96_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005250978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEU5112743.1"
                     /translation="MPVFETPEQISVRLDLGVADVRLVASERADTVVEVRPSHQSRGS
                     DIKAAEQTRVEYSNGRLTVRMAKDWTRYTPFGPAGSVDVIVELPAGSDVHGASDMGSF
                     EADGRLGECNFRSGMGNVRVDRTGPLTVSTGMGEIIADHVAGAAELRTGSGQVRIGVI
                     DGAASVRNGNGETRLGDVAGDLRVKAANGDITITTSRASLVAKSANGNVRVGEVMSGA
                     IVLETAAGEVEVGIHEGTAAYLDVKTQAGNVRSSLQASNPPEPSGSTVEVRARTSYGD
                     IVITRAPA"
     gene            complement(3491..4000)
                     /locus_tag="VFU96_05460"
     CDS             complement(3491..4000)
                     /locus_tag="VFU96_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009187898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112744.1"
                     /translation="MNLTQHVDTLRRELLVAAEAGGDEARALAERLVMPLDSATRLVL
                     LEALSDAASEITSELAPGSVEVRLRGREPEFVVTSPPAEHAFDDVGVSGIETSAGYSA
                     EIDEGSASRTTLRLPDHVKARVDEAAGREGISVNAWLVRAVVAALEPGARDRRRSPSM
                     GQQYTGWVQ"
BASE COUNT          708 a         1325 c         1296 g          737 t
ORIGIN      
        1 gtggcccagg tgtccaccat ggtgcccgag ccgacgtagg caccgatgtt cacgaagccg
       61 ggcatcacga tcactcccgg ctcacagaac gccccgtaac gcaccgtgcc cggcgggacc
      121 acccgaaccc cctgtccggc caagccggtc ttcaagggga tcttgtcgtg atactcgaac
      181 gggcccactt cgagggtctc catctcacga agacggaaat agaggaggat cgcctctctg
      241 acccactcgt tcaccatcca atccccgccg accttctccg cgactcgcaa ctcaccccgg
      301 tcgaggcccg cgatcgtctt ctccacgtcc tcgcgggcgg tctccggctc gacctggccg
      361 gtgaaggcgg cacgaatggt gtcttggtcg ggcacggtgg ctccctgatc ggtctggcgg
      421 cagggtaatt gggtcggctc agcgctacgg tgccagccga tgccctatcg gctgctctac
      481 atcgggctcg gcctgctcgc cgtggccgcc atcgcgctcg gagtcgcctt caaccggagc
      541 ggcgacgagt tggtgctgcc cgaggagatc gaggcgatct cgcccagccc aggggacctg
      601 gtgcccccgc aaaccagtgt cgaggtcgac ctgccggttg cctaccgggc cgacatctat
      661 gtcgacgact ggctggtcac cgacgtggtc ttcgtcgaag gcaccggcgt ctaccggtgg
      721 tcgccatcac ccaacagctc gacgatcacc gaatgggccc ccggcgagca cacggtccgg
      781 gtggtgtggg acacgatcag cgggctcccc gaccctggtg agtacgaatg gtccttccga
      841 gtcggctgag ggtcacgacg cctggccccg gttcggggcg tcatgcgaca gcctggggcg
      901 ggtcgctccg gcgataggcg accagagcca gtgtgtagga gaccacgagg atccccaccg
      961 cccaggccag cgccacccag aagcgaggac cgggggtccc atcggtgagc agtgagcgaa
     1021 tcgtgtccac tatcggtgtc agcggctgat tctccgcgaa gctctgcaac cacggcgcca
     1081 tcgaggcggt cgggacgaac gaaggactga tgaagatcag caggaacagg atgtagctga
     1141 atgcccccgc accctcgacc gacttggcca gcagtccgaa gaacatggcc agccaggtgg
     1201 tggcgagtat gaacaggagc acgaggcctg cggccagcag ccaggcgccg gcgttgggga
     1261 ccgggcgaaa cccgatcaag aacgccacca cgatcaccag gacgcaggag agcgcgttcg
     1321 acaccgtcga tgacaatgct tgtccgctca acaccgacga aggtctcacc ggcatcgtcc
     1381 ggaaacgact gatgattcct ttctgcatgt ccgtggcgag ccgaaaggcg gcatacgcga
     1441 tcccgctgat caccgtcatg atgacgacgc caggcgtcag gaagtcgatg taatccaccg
     1501 aggtctgcgc tccgagggcg cctccgaaca cgtacacgaa gagcagcatc agcgcgattg
     1561 gcatcaggac aacagtgatg atcgtgtcca tgctgcgggt cgtgtgacga aggcttcgct
     1621 cggccatcgt ccaggtgtca gtgagagttc gcatgagttt cctccttgtc ttctccgagg
     1681 atctggaaga agacgtcatc gagagtcggt tctttcgcaa tgaatcgcgt tggctccacg
     1741 ccggcgttgt tgagtcgaat gaacatgtcg gccatgtcag ccacagagcc atcgctggcg
     1801 atgaccagct tcgaatccac ccggctcagc tcatgcccac ttgtcagcgc tctctgagcc
     1861 actgccaact cgttgtcttc ggcgaaatca agctcgacca gcccgctcgg caccattgtc
     1921 ttcagctctt ccggtgtccc gccggccacg accctgcctc cgtggaggat ggcgatctgg
     1981 tcggcgagac ggtcggcctc ctccaggtac tgggtggtga ggaagatggt ggtccccttc
     2041 tcggccagct cccggatggt cagccacatg tcgttccgac tctgtgggtc gagcccggtc
     2101 gtcggctcgt cgaagaagac gatgggcgct tcgcccacca ggctcatggc gatgtcgagc
     2161 cgacgccgca tcccacccga gaaggtgagc aggcgcctgt ccgccgcttc gttgaggtcg
     2221 aacttgtcga gcaaggagga ggcgacctgt tcgggatcgt cgacgtggcg tagctcggcc
     2281 atcagcatca ggttctctcg ggcggtgagg atgtagtcga cagcggcgga ctggccggtg
     2341 acacttatca cctcacgaac gctcgccggc tgcgtcgcca cgtcgaaccc ggcgacgctg
     2401 gctgtgcctc cgtcggcctt gatcagtgtg gtcaggatgt tgatggtcgt cgttttgccc
     2461 gacccgtttg ctccgaggag tgcgaacacg gtgccctgtt tcactgtgaa cgagacgtcg
     2521 tgcagcaccg gcacgtcctt gtaggccttc tcgaggtttc tcacctcgat tgcgttgatc
     2581 tcatgcatgg ctctcattgg ctccttcatg gtttccttcg aataggtgag cgctcatgca
     2641 ggcgctcggg tgatgacgat gtcgccgtag gaagtgcggg cgcgcacctc gacggtcgag
     2701 cccgacggct caggcggatt ggaggcctgc aaggagctgc gaacgttccc ggcctgcgtc
     2761 ttcacgtcca ggtatgccgc ggtaccttcg tggatgccga cctcgacctc gccggcggcc
     2821 gtctccagca cgattgcgcc actcatgacc tcgccgacac gaacgttgcc gttcgccgac
     2881 ttggcgacga gggaagctcg actcgtggtg atggtgatgt ctccattggc cgccttgacc
     2941 cgaaggtcgc cggccacgtc accaagcctg gtctcgccgt tcccgttcct gaccgatgcc
     3001 gcgccgtcga tgacgccgat tcgaacctgg cccgacccgg tcctcagctc ggcagcgccg
     3061 gcaacgtgat cggcgatgat ctcgcccatc ccggtgctga cggtgagagg accggttcga
     3121 tcgactcgaa cgttgcccat cccgcttcgg aagttgcatt ccccgagtcg gccatcggcc
     3181 tcgaatgacc ccatgtcgga ggccccatgg acgtccgagc ccgcgggcag ctcgacgatg
     3241 acgtcgaccg agcccgccgg gccgaagggg gtgtaccggg tccagtcctt agccattctc
     3301 acggtcaatc ggccgttcga gtactcgacg cgggtttgtt cggccgcctt gatgtcggat
     3361 ccccgggatt ggtggctggg ccgcacctcg acaacggtgt cggcgcgttc ggaggcgacg
     3421 agtctgacgt cggcaacgcc gagatcgagt ctgacggaga tttgctcggg agtctcgaaa
     3481 acaggcatgg ctactgcacc catccggtgt actgctgccc catcgaggga ctgcgacggc
     3541 ggtcacgagc acccggctcg agagcggcga ccacggcgcg caccagccag gcattgaccg
     3601 agataccttc ccggccggcg gcctcgtcga cacgagcctt gacgtggtcg ggtaggcgaa
     3661 gggtggtgcg tgacgcactg ccctcgtcga tctcagcgga atatccggcg ctggtctcga
     3721 tgccgctgac gccgacgtcg tcgaacgcat gctcggcggg cggcgaagtc accacgaatt
     3781 cgggctcgcg gccgcgaagc cgcacctcga ccgagcccgg ggcgagctcg ctggtgatct
     3841 cgctggctgc gtccgacagt gcttcgagga gaacgagccg ggtggccgag tcgagcggca
     3901 ttacgagccg ctcggccagg gcccgcgcct cgtcaccacc ggcttcggcg gcgaccaaca
     3961 gctcccggcg cagggtgtct acatgttggg tcagattcat gacaccacta tgacaccagt
     4021 tgtggtgtct gtcaagacac caacccgaca tcaatgacaa tcgact
//
LOCUS       DASTXO010000256         4720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_789879, whole genome shotgun sequence.
ACCESSION   DASTXO010000256 DASTXO010000000
VERSION     DASTXO010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789879"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..851)
                     /locus_tag="VFU96_05465"
     CDS             complement(99..851)
                     /locus_tag="VFU96_05465"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEU5112745.1"
                     /translation="MKWTTRRRNIALIGGLAVLLAACASEAGSTPQEITVNTSEEATS
                     GISVSGMGEVTGAPDTVEVGLGVSVLGETVDQAATTAAEKAQAVIDALTSNGVAEEDI
                     TTTDYSIYPEYDYSSNEERLIGYRVTNTVKAKIRDLDSTGTVLDAATVAGGDDVVVNG
                     LSFSIEDNDELVAAAREAAWNDALTKANQLAELSGQSLGEATTITETVSMPPIPIPYA
                     AEAAGDRALETPIEPGTSAVTITLQVQFALAG"
     gene            complement(875..1153)
                     /locus_tag="VFU96_05470"
     CDS             complement(875..1153)
                     /locus_tag="VFU96_05470"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEU5112746.1"
                     /translation="MTAFEDAVLEVIRSLEFGEVVTYGEVADQAGFPGAARAVGTVLR
                     NCDGDIPWWRVVGAGGLIRTPGKEKQADLLRSEGVLVLEGKVVDSRNR"
     gene            complement(1150..1770)
                     /gene="upp"
                     /locus_tag="VFU96_05475"
     CDS             complement(1150..1770)
                     /gene="upp"
                     /locus_tag="VFU96_05475"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005957347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEU5112747.1"
                     /translation="MPVTILDHPLAADLLSILRSKDTEPPEFRQIAERLGYLLVAEAL
                     SDMSTVDIEIETPLEKTSGRQLRRPVVAVAVLRAGLGLLESVLTLVPDAAIGFAGVQR
                     NEETAEPIEYYTKFPHLSSARVLILEPMLATGGSLSWAIDKVKENGAKDITAVCVVTA
                     PEGLAVIEENHPDVRIVAAALDRELNPSFYIAPGLGDMGDRLFGTL"
     gene            complement(1834..4542)
                     /gene="ppc"
                     /locus_tag="VFU96_05480"
     CDS             complement(1834..4542)
                     /gene="ppc"
                     /locus_tag="VFU96_05480"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018218596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEU5112748.1"
                     /translation="MTPTSDETLRADIRRLGNQLGDALVRQHGPELLDLVEEVRALGK
                     SSRRGGSTEAARQLDRRLADLEVDEVIPLVRAFTTYFYLANVAEQVHRIDQLSIDELG
                     LAATVDRVLEAGVESDVLGEVVRRLEMRPVLTAHPTEAARRSILTKTASLAELIGRRL
                     TASDADVALVDRRTAELIDQIWQTDELRLEKPNPVEEARSALYYLVQIAAEVMPSLSE
                     RVALEIARLGVVTDKSPIRFGSWVGGDRDGNPGVTPETTLETLLLQHDRGIRHLIGLI
                     EDLADELSVSDRLAPMSDEMEASLMSDRDLLPEVWQRESRRTAAEPYRLKCSYIRARL
                     VNTNRRIQQGLGHVEGLDYAHPDALIADLDMMAASLTRARGELIAHGAVSRVRRAVET
                     FGFQMAILDVREHAGKHHQAIAELFSLVDVDYQALAADQRFDVLSAELEGSRPLSGPT
                     TTLSEQADLTLGTFRAIKEAQDLYGDQVIESYIISMTESPADVLAAAVLAREAGLIDL
                     RRGIARIGLVPLVETIDDLRGAGELLDSLFDHAGYRQILEIRGNSQEVVLGYSDSNKE
                     GGITTSQWEIYKAQRSMRNVSERHGVHLRLFHGRGGTIGRGGGPTHAAILAQPFGTVD
                     GELKVTEQGEVISEKYGHPGIAARNLELALSAVLESSLLHRRSRKPKEALDRWTEAMD
                     FFSAEAYASYREFVQHPSLVPYFLTSTPVEELGRLNLGSRPSRRPGGVGGIEDLRAIP
                     WVFGWTQTRQIVPGWFGVGTGIRAARKAGFGDVLEEMAREWGFFQTFLSNVEMTLAKT
                     DLEIAGRYVASLVREEHRGLLDLIRAECELATEEVLALRRQPDLLGDLPLLRRTLGVR
                     DVYLDPISYLQVSLLARSREGEVGDDLDRALLLTVNGIAAGMRNTG"
BASE COUNT          769 a         1540 c         1546 g          865 t
ORIGIN      
        1 catccacctc gaccatgtct ggggcggcgt tcgcctcgcc gacacctgaa accgtgatgc
       61 ccatgatcaa ctccgatcgc ccgcggggag cctatccctc agccggcgag agcgaactgg
      121 acctggagtg tgatggtcac cgccgacgtt ccaggctcga ttggagtctc cagggcccga
      181 tcaccggctg cctcggcggc gtaagggatc gggatcgggg gcatgctcac ggtctccgtg
      241 atcgtggttg cctctccgag gctctgcccg gacagctcgg cgagctggtt ggccttggtc
      301 aatgcgtcgt tccacgccgc ctctctggcg gcagcgacca attcgtcgtt gtcctcgatg
      361 ctgaaggaga gcccgttgac gacgacatcg tcgcctccag cgacggtcgc tgcgtccagg
      421 actgtgcctg tgctgtcgag gtctctgatc ttggccttga cggtgttcgt cacccggtac
      481 ccgatcagcc gttcctcatt gctcgagtag tcgtactcgg ggtagatcga ataatcggtg
      541 gtcgtgatgt cctcctcggc gacgccattc gaggtcagcg catcgatcac cgcctgggct
      601 ttctcggctg ccgtggtcgc ggcctgatcg accgtttcgc ccagaaccga cacaccgagg
      661 ccgacttcga cggtgtccgg cgctccggtc acctcgccca tgcccgagac cgagatgccg
      721 gatgttgcct cctccgatgt gttcacggtg atctcctgtg gagtcgaccc ggcttcactg
      781 gcgcaggcgg ccagcaggac ggccagaccg ccgatcaatg cgatgttgcg acgacgtgtt
      841 gtccatttca tgccggttgg acgaaagagc ccgctcaacg gttcctggaa tcgacgacct
      901 ttccttccag gacgagaaca ccctcggatc tgagtagatc ggcctgtttc tccttgcctg
      961 gcgtccgaat caggccaccg gcgccgacca ctcgccacca cgggatgtca ccgtcacagt
     1021 ttcggagcac cgttcccacc gcgcgggcag caccggggaa tcctgcctga tctgccacct
     1081 cgccgtaggt gaccacctcg ccgaactcga gtgacctgat gacttcgagc acggcgtcct
     1141 cgaaagcggt cacagagtcc cgaacagacg gtcccccatg tcgccgagac cgggtgcgat
     1201 gtagaagctg gggttgagct cgcggtcgag ggcggcggcg acgatccgta catcgggatg
     1261 gttctcttcg atgaccgcca atccctcggg cgcggtgacg acgcacaccg cggtgatgtc
     1321 ctttgccccg ttctccttga ccttgtcgat tgcccaggac agggagccgc cggtggcgag
     1381 catcggctcg aggatcagca ctctggccga ggagagatgg ggaaacttgg tgtagtactc
     1441 gatcggttcg gccgtctcct cgttgcgctg caccccggcg aagccgatcg cagcgtccgg
     1501 taccagcgtg agcaccgact ccaagaggcc gaggccggct cggaggacgg ctacggcgac
     1561 taccggccgc cgaagctggc gaccgctggt cttttcgaga ggggtctcga tctcgatgtc
     1621 caccgtcgac atgtcggaga gcgcctcggc caccaacagg tagccgagcc gttcggcgat
     1681 ctggcggaac tcgggcggct cggtgtcctt gctacgcagg atggacagca agtcggcggc
     1741 gagtgggtgg tcgaggatcg tgacgggcat gacggccaac ctatcaattc gcggattggg
     1801 gggggtcgag gcggatgatc cggccgaccg gcgtcagccg gtgtttctca ttccggctgc
     1861 gatcccattc acggttagta ggagggcgcg gtctagatcg tctcctacct caccttcccg
     1921 gcttctggcc agcagcgaca cctggagata gctgatcggg tcgaggtaaa cgtcacgcac
     1981 cccgagtgtc cgccgcaaca gcggcagatc gccgagcagg tcaggttgcc ggcgcaaggc
     2041 gaggacctcc tcggtggcca attcgcattc ggcccggatc agatcgagga gcccgcgatg
     2101 ttcctctctg accagactgg ccacatagcg gccggcgatc tcgagatctg tcttggccaa
     2161 tgtcatctcg acattggaga ggaaggtctg gaagaagccc cactcacggg ccatctcctc
     2221 gagcacatcc ccgaacccgg ccttcctggc cgcccggatc ccggttccaa ccccgaacca
     2281 gccgggcacg atctggcggg tctgggtcca gccgaacacc cacgggatgg cccgcaggtc
     2341 ctcgatgccc ccaacgccgc cagggcgacg cgacgggcgg ctacccaggt tgagccgtcc
     2401 gagctcctcg accggggtcg aggtgaggaa atagggcacc aggctcgggt gctggacgaa
     2461 ctcgcggtag gacgcgtaag cctcggcgga gaagaagtcc atggcttcgg tccatctgtc
     2521 cagggcttcc ttcggcttgc gcgagcgccg gtgcagcagc gacgactcca gcaccgccga
     2581 cagtgccagc tccaggttgc gggccgcgat gccggggtgc ccgtatttct ccgagatcac
     2641 ctcgccttgc tcggtgacct tgagctcacc gtccacggtg ccgaatggct gggcgaggat
     2701 ggcggcgtgg gtgggcccgc cgccacggcc gatggtcccg ccgcggccgt ggaacaaacg
     2761 caagtggacc ccatgccgct cggagacgtt ccgcatcgac cgctgggcct tgtagatctc
     2821 ccactgcgag gtggtgatcc ctccctcctt gttggagtcg gagtagccga gcacgacctc
     2881 ctggctattg ccccgaatct cgagtatctg gcggtatccg gcatggtcga agagagaatc
     2941 gaggagctcg ccggcgccac gcaggtcatc gatggtctcc accaggggca ccaggccgat
     3001 ccgggcgatg ccgcgcctga ggtcgatgag gcctgcttcg cgtgcgagta ccgcggccgc
     3061 gaggacatcc gccgggctct ctgtcatcga gatgatgtat gactcgatga cctggtcccc
     3121 gtagaggtcc tgcgcctcct tgatggcgcg gaaggtgccg agggtgaggt ccgcctgctc
     3181 gctgagggtg gtggtcgggc cactcagggg ccggctgcct tcgagctcgg ctgacaacac
     3241 atcgaagcgc tggtcggccg ccagtgcctg gtagtcgaca tcgacgagtg agaacagctc
     3301 ggcgatggct tggtgatgct tgcccgcgtg ctcgcgcacg tcgaggatcg ccatctgaaa
     3361 tccgaacgtc tccacagccc ggcgcacccg ggacaccgcg ccgtgtgcga tcagctcgcc
     3421 acgggcccga gtgagggatg cggccatcat gtcgaggtcg gcgatcaggg catccggatg
     3481 tgcatagtcg agaccctcga catgtccgag accctgttgg atccggcggt tggtgttgac
     3541 caggcgggcc cgtatgtagg agcacttgag gcgatacggc tcggctgccg tccgccggct
     3601 ctcgcgctgc cacacctcgg gcaggaggtc gcggtccgac atcaacgacg cttccatctc
     3661 gtcactcatc ggagcgagac ggtcggacac ggagagctcg tcggccaggt cttcgatcag
     3721 cccgatgaga tgccgaatac cgcggtcgtg ctggagcaag agcgtctcca gggttgtctc
     3781 cggagtcacg cccgggttcc catcccggtc tcctccgacc cacgacccaa atcggatcgg
     3841 gctcttgtcg gtgaccacgc cgagtcgggc tatctcgagc gccacccgct cactcaggga
     3901 gggcatcacc tcggctgcga tctgcaccag gtagtagaga gcggagcgag cctcctccac
     3961 cgggtttggc ttctcgagcc gcaactcgtc tgtctgccag atctggtcga tcaattccgc
     4021 agtgcgccgg tcgacgaggg cgacatcggc atcactggcg gtcagacgtc tcccgatcag
     4081 ttcggccagg ctcgcggtct tggtcagtat cgagcgtctt gcagcctcgg tgggatgcgc
     4141 cgtgagcacc ggccgcatct cgagtcttcg cacgacctcg ccgagaacat ccgactcgac
     4201 gccggcctcg aggactcggt cgacggtggc cgccaatccg agctcgtcga tgctcagctg
     4261 atcgatgcgg tggacctgtt cggccacgtt tgccaggtag aagtaggtcg tgaacgccct
     4321 gaccagaggg atgacctcgt cgacctccag atcggcgagc cggcggtcga gttggcgggc
     4381 agcctcggtg ctgccgcctc tgcgggagga cttacccagc gccctcacct cctcgacgag
     4441 atcgagcaac tcggggccgt gctggcggac gagggcatcg ccgagctggt tgccgagacg
     4501 tctgatgtcg gcccggagcg tctcgtctga cgtgggtgtc accggggata gggtattccg
     4561 ccggggggca cgcctacccg cctattcgtc ctcggctcgc tgcttgtgaa ccctgccatc
     4621 gattccgtgc aatgagaggc cggtgccatg gccgtagcgc accatggtca cctcggccag
     4681 gatggacacg gcgacctcct gggggctctc ggcaccgatg
//
LOCUS       DASTXO010000257         7717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_900245, whole genome shotgun sequence.
ACCESSION   DASTXO010000257 DASTXO010000000
VERSION     DASTXO010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7717
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900245"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VFU96_05485"
     CDS             <1..260
                     /locus_tag="VFU96_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013345499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEU5112749.1"
                     /translation="VAVFEATHGTAPKYANQDKVNPGSVVLSGEMMFRYLGWTEAADL
                     IIRGLEGAIKARTVTYDFARLTDDATEVKTSEFGDAIIGHM"
     gene            359..1327
                     /gene="nadA"
                     /locus_tag="VFU96_05490"
     CDS             359..1327
                     /gene="nadA"
                     /locus_tag="VFU96_05490"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEU5112750.1"
                     /translation="MTTLQITRPSDVLPEEEVAALAPVLDDIERLKVERNAIVAAHNY
                     MTPDIYHLAADITGDSLALAQLALDTEAEVIVLAGVHFMAETAKIINPEKMVLIPDLE
                     AGCSLADGITGADVRHLKEQHPGVPVVTYVNTSAEVKAESDITCTSSNAVAVVESLGV
                     DRVIFTPDQYLGRWVATQTDVDVILWEGSCIVHERFTGEEIADYRRAYGDLPVIAHPE
                     CPPDVLAEADFVGSTHAMIDWVTTRQPSRVMMITECSMADNVARETPHTEYVRPCNLC
                     PYMKRITLDNIRTSLATLTHQVEIASEVAERARRAVMRMLEVGRKD"
     gene            1340..2857
                     /locus_tag="VFU96_05495"
     CDS             1340..2857
                     /locus_tag="VFU96_05495"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEU5112751.1"
                     /translation="MTEITRLDAPLVVGAGVAGLTVALGLPRAYVLTTQEMGSTWWAQ
                     GGIAVAWSPEDSPRQHADDTLNVSGGLGVERVVDILTQGGPAAVENLIAIGAQFDRDE
                     EGELMLAREGGHQTRRVLHADGDATGAEVMRALIAAVEADDRIRILQGRAIDLVMAGD
                     RVIGVVTSEGKKREVLLAPAVVLATGGAGRLYSQTTNPSGVTGDGIAMAGRAGARLAD
                     LEFVQFHPTALHVGKDPMPLLTEALRGEGAILVDAHGRRFMPQYHPMAELAPRDIVAR
                     AIFWQYDRGLGGAYLDARSILDFADRFPTVNAHAHSVDLDPSEHLLPVSPAAHYYMGG
                     IDADANGRTSVPGLWAVGESSSTGVHGANRLASNSLLEGLVFGARASADVAESLVEPD
                     GEPFAPKEAFDLPLVAGPALEDLRQIMWDRVGLVRTGPGLWEARSHLLELDSVLRRTI
                     AGRVALELSLMVILAALRRSESRGGHYRADYPEPDPMQEHRTLIDPTRAQMTAVS"
     gene            2857..3690
                     /gene="nadC"
                     /locus_tag="VFU96_05500"
     CDS             2857..3690
                     /gene="nadC"
                     /locus_tag="VFU96_05500"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEU5112752.1"
                     /translation="MLRTDYVEVVGRALAEDLGSDGDITSDSCVPQTAESKGVFVARG
                     RGVIAGLEVAAYVFEALDPSVRFEPKVEDGHPVEEGEIIAHISGHSRSILSGERTALN
                     LLGRMSGVATATAELVEAVDGTGARISDTRKTMPGLRVLDKYAVRVGGGMNHRFGLYD
                     AVLIKDNHLVAAGDIADAVTAARANVRPGVMIEVEVVSLEQLAQVLQTDADRVLLDNM
                     GPELLRQAVEMADGRITLEASGGVTLENVRLLAETGVDIISVGWITHSAPQLDIALDF
                     M"
     gene            3718..4707
                     /locus_tag="VFU96_05505"
     CDS             3718..4707
                     /locus_tag="VFU96_05505"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEU5112753.1"
                     /translation="MSDQPVRVAVTGAAGQIGYSLLFRIAAGDMLGPDQPVILQMLEI
                     PPALGALEGVTMELADGAFSLLEGTVTTDDPETAFADADIALLVGAMPRKAGMERSDL
                     LSANGAIFTTQGRAIAAAARPDVKVLVVGNPANTNALIAMNNARGMDRSNFTAMTRLD
                     HNRAVAQLSKRVGYPVSSIRRMIIWGNHSSTQYPDLFHCEVDGKAADELVDDQGWIDN
                     EFIPTVADRGAAIIEARGASSAASAANAAISHVRDWWEDTPDGDWVSMGVPSDGSYGV
                     PDGLMCSFPCITRNRRWEIVQGLEIDDYSRKRIDASVAELIEERDAVKKLGLI"
     gene            complement(4718..5095)
                     /locus_tag="VFU96_05510"
     CDS             complement(4718..5095)
                     /locus_tag="VFU96_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU5112754.1"
                     /translation="MPKYAALIYTSAEEQGQGTPEELEQVMNEYNDFTRAAAEAGVMV
                     GGEALHDTNTATTVHIPGGKGGEPVFTDGPFAETKEALGGFYLFDVADLDEALKWAQQ
                     IPGAWYGRVEVRPIVEFEDQPEG"
     gene            complement(5179..5562)
                     /locus_tag="VFU96_05515"
     CDS             complement(5179..5562)
                     /locus_tag="VFU96_05515"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEU5112755.1"
                     /translation="MSKFLSDEWASEVTAALNSHDGFKNAIGEAELGIQFNTTDAPDG
                     EVDYYLQAGGGNATMALGTLEGADVTVKQTYDTATAISKGDLNTQTAFMTGKLKVSGN
                     LAKLMMHQGAIQQWGSAVSDLDVEY"
     gene            complement(5636..6457)
                     /locus_tag="VFU96_05520"
     CDS             complement(5636..6457)
                     /locus_tag="VFU96_05520"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU5112756.1"
                     /translation="MITPFTRGGEIDTRAHTTNLRLLVGAGIDGFVIGGSNGEGPYLE
                     PGERFRLVKAGRRRKSHFMVGIAAESTRVALNQLEEAIEGGADSILVMTPTTLTRGRD
                     EAVLRFYKTIADTSTTPIFLYSVPSVTGYSLPLELVWRLSRHDNIVGMKDSSGDVVRL
                     QSIIDASPSDFLLYSGSSRALTAAMAVGCHGAITGSGNYVPELVLQTLAAAKTDTVKA
                     RKLQKRLSAITADVEAHGVPGVKAAAKAAGLDPGHPREPLTRLSRGAETSIAKLI"
     gene            complement(6513..>7717)
                     /locus_tag="VFU96_05525"
     CDS             complement(6513..>7717)
                     /locus_tag="VFU96_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112757.1"
                     /translation="VSVQDVVPTFFLGAIGFDTMTIERSAIAEYIPPLKLGSPAGQFG
                     NSCDPTQAGCTSQPNFWANIHGWFTDTTMGDAYASHCVGSSDVPTCTANAFARPGGYL
                     YGIESTGSFTVQGLDLRFFNTSGGNPTSDTIRTGDRGCEDWTPSSAGSCGPTMLVRLY
                     APDPTPLDLSDNAAPLCTATLAPTGQVLPAAAYTWATPASGASAGSSPSTCWTRSGTG
                     IYVLQITMANPGATVDRAGLNRYAIRANGTGAKLFALGDFSIYNNASGTTTSFYLAEV
                     PTYYHGKTFVVELYDAGESASNGTLAVVDPGGSTFNDGECRIYSRNNPSAAWSLQSTI
                     PSGSNCQETVTPGEYNGRWLKFEMDLPASYSCSTCWWKMSYAYPTAVNDTTTWRAYMI
                     GNPIHLID"
BASE COUNT         1324 a         2451 c         2539 g         1403 t
ORIGIN      
        1 gggtagctgt cttcgaggcc acccacggca ccgcccccaa gtacgccaac caggacaagg
       61 tgaatcctgg ctctgtcgtt ctctccggcg agatgatgtt ccgctatttg ggctggaccg
      121 aggcggcgga cttgatcatc cggggtctgg agggtgcgat caaggccaga acggtgacct
      181 acgacttcgc ccgtctcacc gacgacgcca cggaagtcaa gacctccgag ttcggcgatg
      241 ccatcatcgg ccacatgtag ccatcggggc ggcaaacccg tcaccagtgc tcgccctcat
      301 gaccgggatg gctgagtcgc cgccactcct agactcagaa cattcctagc gggagcgcat
      361 gaccactctt caaatcaccc ggccctctga tgtcctgccc gaagaggaag tggccgcgct
      421 cgcaccggtt ctcgacgaca tcgagcggct caaggtggag cgaaatgcga tcgtcgccgc
      481 ccacaactac atgacgcccg acatctatca cttggcggcg gacatcaccg gagactcgct
      541 ggccctcgcc cagctcgccc tggacaccga ggccgaggtc atcgtcctcg ccggcgtcca
      601 tttcatggcg gagacggcca agatcatcaa ccctgagaag atggtcctga tccccgatct
      661 cgaggccgga tgctctctgg ccgacgggat cacaggcgcc gacgtccggc atctgaagga
      721 gcagcacccc ggggtcccgg tggtgaccta tgtcaacact tcggctgagg tcaaggccga
      781 gtcggatatc acctgcacat cttccaacgc ggtcgcagtg gtggagtccc tgggtgtcga
      841 ccgggtcatc ttcaccccgg accagtatct cggccggtgg gtcgccactc agacggacgt
      901 cgacgtgatc ctgtgggagg gctcgtgcat cgtccacgag cggttcaccg gtgaggagat
      961 cgccgactac cggagggcat acggcgacct gcctgtgatc gctcatcccg aatgcccacc
     1021 cgacgtgctc gccgaggccg atttcgtcgg ttccacccat gcgatgatcg attgggtgac
     1081 cacccgccag ccgagccggg tgatgatgat caccgagtgt tccatggccg acaacgtggc
     1141 ccgcgagacc ccgcataccg agtatgtgcg accatgcaac ctctgtccgt acatgaagcg
     1201 gatcaccctg gacaacatcc ggacctcgct ggccaccctc acccatcagg tcgagatcgc
     1261 ctccgaggtg gcagagcggg cacggcgagc ggtcatgcgc atgctcgagg tcggtcgtaa
     1321 ggactagggg caacctccga tgaccgagat cacccggctc gatgcgccgc tggtggtggg
     1381 cgccggcgtc gccggcctca cagtggcgct cggcctgccc cgggcatacg tcctgaccac
     1441 tcaggagatg ggatcgacat ggtgggccca gggcggcatc gccgtggcat ggtcacccga
     1501 ggattcgcca cgccaacacg ccgacgacac gctcaacgtc tctggggggc tgggggtcga
     1561 gcgggtggtc gacatcctga cccaaggcgg cccggctgcg gtcgagaacc tcatcgccat
     1621 cggtgcccag ttcgaccgtg atgaggaagg tgagctcatg ctcgcccgcg agggtgggca
     1681 ccagacgcgg cgcgtcctgc atgcggacgg ggatgcgacc ggcgccgagg tcatgcgggc
     1741 gctcatcgca gcagttgagg cagacgaccg gatcaggatc ctccagggac gcgccatcga
     1801 cctggtcatg gcgggtgatc gagtcatcgg ggtggtcacc agcgaaggga agaagagaga
     1861 agtgctgctg gcccccgcgg tcgtgctcgc cactggcggc gcggggaggc tgtactccca
     1921 gaccaccaat cccagcgggg tcaccggtga tggcatagcg atggcggggc gggcgggagc
     1981 gcggctcgcc gacctcgagt tcgtccagtt ccacccgacg gcgcttcatg tcggcaagga
     2041 cccgatgcct ctcttgaccg aggccctccg cggcgagggc gccatcctgg tcgacgcgca
     2101 cggccgccgt ttcatgcccc aatatcaccc catggcggag ctcgccccac gcgacatcgt
     2161 ggcccgcgcc atcttctggc agtacgaccg gggtctaggc ggggcctatc tcgacgcgag
     2221 atcgatactc gatttcgccg atcggttccc cacggtcaac gcccacgccc actcggtcga
     2281 tctcgacccg tccgagcacc tgctgccagt ctcacccgcc gcccactact acatgggcgg
     2341 gatcgatgcc gacgccaatg gacggacttc ggtcccgggc ctgtgggcgg tcggcgaatc
     2401 cagttcgacc ggcgtgcatg gggcgaaccg gctcgcctcg aactcgttgc tcgagggcct
     2461 ggtcttcggg gcaagggcgt cagccgatgt cgccgaatcg ctggtcgagc ccgacgggga
     2521 gccgttcgcc cccaaagagg cattcgacct gcctctggtc gcaggtccgg ccctcgaaga
     2581 cctccgtcag atcatgtggg accgggtggg tctggtgcgc accggccccg gtctctggga
     2641 ggcaaggagc catttgctcg agctcgattc cgtgctgcgc cgcaccattg ccgggcgcgt
     2701 cgccctcgag ctgtcgttga tggtgatcct ggctgcgctc cgtcgttccg agtcgagggg
     2761 tgggcactac cgggccgact atccagagcc cgacccaatg caggaacacc gcacgctgat
     2821 cgatccgacc agggcgcaga tgaccgcggt cagttgatgc tgcggaccga ttacgtcgag
     2881 gtagtgggtc gcgccctcgc cgaagatctc ggatccgacg gggacatcac ctcggattca
     2941 tgtgttcccc agaccgcgga gagcaaaggc gtgttcgtcg cccgcgggcg cggggtgatt
     3001 gccgggctcg aggtggcggc atatgtgttc gaggcgctcg atccatcggt gcgattcgag
     3061 ccaaaggtcg aggatgggca tcctgtcgaa gagggcgaga tcatcgccca catctcgggg
     3121 cactcccgct ccatactcag cggggagcga accgcgctca acttgttggg ccgcatgtcg
     3181 ggggtggcca cggcaacagc cgagctcgtc gaggccgtcg acgggacagg ggcacggatc
     3241 tccgacaccc ggaagaccat gccggggctg cgtgtcctcg acaagtacgc ggtcagagtc
     3301 ggcggcggca tgaaccaccg gttcgggctc tatgacgcgg tcttgatcaa ggacaaccac
     3361 ctcgtcgccg ccggcgacat cgccgacgcc gtgacggcgg cccgagccaa cgtgcggccc
     3421 ggggtgatga tcgaggtcga ggtcgtgagc ctcgaacaac tggctcaggt cctccaaacg
     3481 gacgcggatc gtgtgctcct cgacaacatg ggccccgagt tgttgcgtca ggctgtggag
     3541 atggccgacg gtcggatcac attggaggca tccggagggg tcacactcga gaatgtccgc
     3601 ctcttggcgg agaccggcgt ggacataatc tcggtgggat ggatcactca ctcggcgccc
     3661 cagctcgaca tcgcactgga tttcatgtga ctctcagccc gggataactt cggcgtcatg
     3721 agtgatcaac cagtacgcgt cgccgtcacc ggagcggccg gacagatcgg gtacagcctt
     3781 ctcttcagga tcgcagccgg ggacatgctg ggccctgacc agcctgtcat cctccagatg
     3841 ctcgagatcc cgccggcact tggcgcgctc gagggcgtga cgatggagct cgccgacggc
     3901 gccttctctc tgctcgaagg aacagtcacc accgacgatc ccgaaaccgc ctttgccgat
     3961 gccgacatcg cccttctcgt cggcgcgatg ccccggaagg ccggaatgga acgttccgac
     4021 ctcctctccg ccaatggcgc gatcttcaca actcaggggc gagccatcgc cgcggccgcc
     4081 cgtcctgacg tcaaagtgct cgtggtgggc aacccggcga acaccaatgc gctgatcgcc
     4141 atgaacaacg cccgggggat ggatcgttcc aacttcaccg cgatgacccg gctcgaccac
     4201 aaccgggcag tggcccagct ctcgaaacgt gtgggatatc cggtgtcgtc gattcgacga
     4261 atgatcattt gggggaacca ctcgtcgacg cagtatcccg atctgttcca ctgcgaagtc
     4321 gacggcaaag ccgccgatga gctggtggac gatcaggggt ggatcgacaa cgagttcata
     4381 cccaccgtcg ccgaccgtgg agccgcgatc atcgaggcgc ggggggcgtc ctcggcggca
     4441 tcagccgcca atgccgccat cagtcatgtc cgtgattggt gggaggacac acctgatggt
     4501 gattgggtgt cgatgggagt tccctcagat ggctcgtacg gtgttcccga cgggctgatg
     4561 tgctcgtttc cctgcataac ccggaatcgc cggtgggaga tcgtccaggg cctcgagatc
     4621 gacgactact cccgcaagag gatcgacgcc tcggtggctg agctcatcga agagcgggac
     4681 gcggtcaaga agctggggct gatctagccc caagcattca accctccggc tggtcctcga
     4741 actcgacgat cggccgcacc tcgacccgcc cgtaccatgc gcccgggatc tgctgcgccc
     4801 acttcagggc ctcgtcgagg tcggcgacat cgaagaggta gaaaccaccc agggcctctt
     4861 tggtctcagc gaagggcccg tcggtgaaca ccggctcgcc gcccttcccg ccggggatgt
     4921 gaacagtggt cgcggtgttg gtgtcgtgca gggcctcccc gccgaccatc actcccgcct
     4981 ctgcggccgc ccgtgtgaaa tcgttgtatt cgttcatcac ctgctcgagc tcctcgggcg
     5041 tgccttggcc ctgctcctcg gcagaggtgt agatcagtgc tgcgtacttg ggcatcctgg
     5101 tttcccttct cttgcgggac gccttaccga tctatgacgg agagcatgtg cggatctcga
     5161 cattcttcgg ttcgttgctc agtattcgac gtcgaggtcc gacaccgccg agccccactg
     5221 ctgaatggcg ccctgatgca tcatcagctt ggcgagattg cccgagacct tgagctttcc
     5281 cgtcatgaag gcagtctggg tgttcagatc gcctttcgag atggcggtcg ccgtgtcgta
     5341 cgtctgcttg acggtcacgt cggctccctc gagggtgccg agcgccatgg tggcgttgcc
     5401 accgccggcc tggaggtagt aatcgacctc accgtcgggg gcatcggtgg tgttgaactg
     5461 gatgccgagc tcggcttcac cgatcgcatt cttgaagccg tcgtggctgt tgagggcggc
     5521 ggtgacctcc gaagcccact cgtcactgag aaacttggac acgttgtgac tccttggagt
     5581 ttgggggtgt gaatggtaca ggggcgtcgc ctgaccgagt aagccgacct ccctatcaga
     5641 tcagcttggc gatggaggtc tccgccccac gggacagcct ggtcaagggc tccctgggat
     5701 gccctggatc gaggcccgcg gccttcgccg cggccttgac gcccggcacc ccgtgtgcct
     5761 cgacatcggc agtgatggcg ctgaggcgct tctgcagctt cctcgccttg acggtgtcgg
     5821 tcttggccgc ggcgagtgtc tggagcacca actcggggac gtaattgccc gatccggtta
     5881 tggctccatg acagccgacg gccattgccg cggtgagagc tcgggacgac cccgaataga
     5941 ggaggaagtc cgacggcgaa gcgtcgatga tcgactggag cctcaccacg tctccgctcg
     6001 agtccttcat ccccacgatg ttgtcgtggc gggagaggcg ccagaccagt tcgagcggga
     6061 ggctgtaccc ggtgacggat gggacggagt agagaaagat gggggtagtg gaggtgtcgg
     6121 cgatcgtctt gtagaagcga agcaccgcct cgtcccggcc ccgggtcaga gtggtcggtg
     6181 tcatcaccag gatgctgtcg gcgccgccct cgatcgcctc ctcgagctga ttcagcgcga
     6241 ctcgggtcga ttcggccgcg atgccgacca tgaaatggga ctttcgacga cggccggctt
     6301 tcaccagccg gaatcgctcg cccggctcca gatagggccc ctccccgttc gacccaccga
     6361 tgacgaagcc gtcgatgccg gcgccgacca gcaaccggag gttggtcgtg tgcgccctgg
     6421 tgtcgatctc cccgcctctc gtgaagggcg tgatcaacgc gggcatgtaa tcgggagggg
     6481 gcagcatgcg gccagattag ggccgcgatg cctcagtcga tgaggtggat cgggttcccg
     6541 atcatgtagg cgcgccaggt tgtggtgtcg ttcacagccg ttggataggc gtagctcatc
     6601 ttccaccagc aggtggagca ggaatatgac gcaggcagat ccatctcgaa cttgagccag
     6661 cgtccgttgt actcaccggg ggtcactgtc tcctgacagt tgctccctga cgggatcgtc
     6721 gattgcaggc tccatgccgc gctcgggttg ttgcggctgt agatccgaca ctccccgtcg
     6781 ttgaaagtgc tgccgccagg gtcgaccacc gccagggttc cgttgctggc cgactcaccc
     6841 gcgtcgtaca gctccaccac gaaagtcttg ccgtggtagt aggtgggcac ttctgccagg
     6901 tagaaggagg tcgtcgtgcc gctggcgttg ttgtagatcg agaagtcacc gagggcgaac
     6961 aacttggctc cggtgccgtt ggctctgatc gcatagcggt tcagaccggc tctgtcgacg
     7021 gtggcgccag gatttgccat ggtgatctgg agcacataga tcccggtgcc gctccgggtc
     7081 cagcaggtgc tcggagagct tccagctgat gcgcccgaag ctggcgtcgc ccatgtgtag
     7141 gcggcggcgg gcagcacctg cccggtgggg gcgagggttg ccgtgcacaa gggggcggca
     7201 ttgtcggaga ggtcgagcgg tgtggggtcc ggggcgtaga gcctgaccag catcgtcggc
     7261 ccacagctcc cggcgctgct cggggtccag tcttcgcacc cacggtcacc agtgcggatg
     7321 gtgtcactgg ttggattgcc accggaggtg ttgaagaatc tgagatcgag cccctggact
     7381 gtgaaagagc cggtggactc gatgccgtac agataccccc cgggacgggc gaacgcgttg
     7441 gcggtgcacg tagggacatc cgagcttccc acgcaatgcg acgcgtaggc atcgcccatg
     7501 gtggtgtcgg tgaaccatcc gtggatgttt gcccagaagt tcggttggct ggtgcaacca
     7561 gcttgtgtgg ggtcacagct gtttccgaac tggccggccg ggcttcccaa tttcaacggc
     7621 ggtatgtatt cggcgatggc ggaccgctcg atggtcatcg tgtcgaagcc gatggcgccg
     7681 aggaagaagg tgggcaccac gtcctgcacc gacacct
//
LOCUS       DASTXO010000258         3733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1047612, whole genome shotgun sequence.
ACCESSION   DASTXO010000258 DASTXO010000000
VERSION     DASTXO010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047612"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..382
                     /locus_tag="VFU96_05530"
     CDS             161..382
                     /locus_tag="VFU96_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112758.1"
                     /translation="MSRLSRFDSYRYVGTRDTMRFYDCDDESQFDELSGRIAEEGLVG
                     RDMVQVFAPDTPTEAMNRGYRPFAVSDQV"
     gene            complement(368..976)
                     /locus_tag="VFU96_05535"
     CDS             complement(368..976)
                     /locus_tag="VFU96_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112759.1"
                     /translation="MIDRVQLGTSDGETLEARWDRPENPSGAVVFCHPHPQQGGTMMA
                     PLMVAVTTRLVERGFSVLRFNFRGIGASTGTHDDGKAEILDVAAAIGAARDTRLALHV
                     SGWSFGARTALRWLGTQQDPIPYAGIAPSPEDLPDELPPGPKRIVVGTRDQVVDIDGI
                     RAYTERTSIDLVLTPGDHFFHGRGKKIGDLVAQGFEPASNLI"
     gene            1132..2499
                     /gene="lpdA"
                     /locus_tag="VFU96_05540"
     CDS             1132..2499
                     /gene="lpdA"
                     /locus_tag="VFU96_05540"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015749222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU5112760.1"
                     /translation="MHDIVIVGGGPGGYAAALYAENFGLSVALVDEERPGGTCLIRGC
                     IPAKQWLYVAEVFSTVSHAAEFGVVTEQPTLDWEAALARKTQTVEGLVRGLGGLLKKK
                     GVEVITGYGRLAGPGKVEVETDEGKSTIEGGAVILASGSYARTLPGYDFDGDKIVSSD
                     HALDWPQRPNRVAIVGGGVIGCEFASFLVDVGTEVHVFEVMDQIIPGSDADASKQLQR
                     QLQKRGVKFHIGVGVDAPKVGETVVVPFGEESVEVDVVLVSVGRGPRTDDVGLDTVSV
                     KLDRGFVEVDLNTMQTAQPGLYAVGDIVAGTPQLAHVGFAEGIAAVTHIGTGRARPVD
                     YRAIPRVTYTHPEVAEVGLTETEATEKGLEVASAKHALNGIGRAIIIGDNQGFAKVIS
                     EKDGPIVGAAVVGPHAGELIHELMYAVGWEALPDEAAAFIHAHPSLSEVVGETLMSAA
                     GRSLH"
     gene            2511..>3733
                     /locus_tag="VFU96_05545"
     CDS             2511..>3733
                     /locus_tag="VFU96_05545"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEU5112761.1"
                     /translation="MATTVSMPQLGETVTEGTILRWAKQVGDTIAEDEVLVEISTDKV
                     DTEVPSPAAGTILEILVNEGDTVSVGTPMVVIGAEGEGSPAAEEQAAEALAEEQPAAP
                     SGEEEPAEPAAEEQPSEPSADDEPVEPATEEKPIEPEAASTAAEKPEEPTPAKDEKPT
                     EPEVEAFFAKPDETETKPNLLSPVVRKLIREHEVDVSQLSGTGDGGRITRKDVEAYLA
                     SGPSVAEEAAPQPAPTSEPAPPAAGEPQPTPEPAAAPQPAPVAAAAAGGQGADEIELD
                     RIRVRTAENMIKARQTAAHVWTSVEVDYERVERVRQAHKQEFKAAESFSLTYLPFIAR
                     ATIDALRAFPVVNSSFHLEEKKSVIHRNVDLGIAVDLDQSGLVVARVRNADGLRLVGI
                     ARSIRELADKARNGKL"
BASE COUNT          716 a         1176 c         1257 g          584 t
ORIGIN      
        1 ccccaggtca acccccgccc ccaccgccgc cccctccgcc gctgccacgc atcgaatgcc
       61 ccgaatgcga cttcagcttc atcgtcggtg acatcgcggt agcgacatgc cccaattgcg
      121 gggcagaagt gagaaccggc cggggagact cggaggagga gtgagccgtc tctcccgttt
      181 cgactcctac cgatatgtgg ggaccaggga cacgatgcgg ttctacgact gcgacgacga
      241 gtctcaattc gacgagctct ccggtcggat cgccgaggag ggtctggtcg ggagggacat
      301 ggtgcaggtg ttcgctcctg acacgccgac ggaggcgatg aaccgcgggt accgaccgtt
      361 cgcggtctca gatcaggttt gacgcgggct cgaaaccctg cgccaccaga tcaccgatct
      421 tcttgccccg accgtggaag aaatggtcgc ctggggtgag aaccagatcg atggaagttc
      481 gctcggtgta ggcacggatc ccatcgatgt cgaccacctg atcccttgtc ccaaccacta
      541 tgcgctttgg gcctgggggc agttcatcgg gaagatcttc cggggacggt gcgatcccgg
      601 cgtaggggat cggatcctgc tgggtgccga gccagcgcag cgcggtccgt gccccaaacg
      661 accatccgga gacatgcaag gccagcctgg tgtcacgagc ggccccgatc gcggcagcga
      721 cgtcgaggat ttcagctttg ccgtcgtcat gcgtcccggt cgatgcgccg atcccccgga
      781 agttgaaacg gagcacagaa aagccccgct ccaccagtct cgtggtcaca gccaccatca
      841 atggcgccat catcgtccct ccctgctggg ggtggggatg acagaacacc accgcgccgg
      901 acgggttttc ggggcggtcc caccgcgctt cgagcgtctc gccatccgaa gtgcccaact
      961 ggacgcggtc gatcatgtga acgccacaaa cagccatatg gcggccgccc ccaggatcac
     1021 gaagccgagc accgcagttg cgatgagaag ccaacgcgtc tgcatgggtt cagggagggt
     1081 aaccatgggt acaactagtc tcggaccagc agttccaccg aaaggcacag ggtgcacgac
     1141 atcgtcatag tcggcggcgg gccggggggc tacgcagccg ccctctacgc ggagaacttc
     1201 ggtctgtccg tggcgctcgt cgacgaggag cgtcccggag gcacctgtct catccgtggc
     1261 tgcatccccg caaagcaatg gctctacgtg gccgaggtgt tctccacggt gtcgcacgcc
     1321 gccgagttcg gagtcgtgac cgaacagccc acgctggact gggaggcggc actggcccgc
     1381 aagacccaga cggtcgaggg gctggtgcgt gggctcggtg ggctgttgaa gaagaaaggc
     1441 gtcgaagtca tcaccggcta cggccggctt gcggggccgg gaaaggtcga agtcgagacc
     1501 gacgaaggaa agagcacgat cgaaggcggg gccgtgatcc tcgccagcgg ctcctacgcc
     1561 cgcacccttc ccggctacga cttcgacggc gacaagatcg tcagctccga tcacgccctc
     1621 gactggcctc agcggcccaa ccgtgtcgcc atcgtcgggg gtggcgtgat cggatgcgag
     1681 ttcgcctcct tcctggtcga cgtcgggacc gaggtgcatg tcttcgaggt gatggatcag
     1741 ataattcctg gctccgatgc cgacgcctcg aagcagctcc agagacagct ccaaaagcgc
     1801 ggcgtcaagt tccacatcgg cgtcggcgtc gacgccccca aagtcggcga gacagtggta
     1861 gtgccgttcg gtgaggagag cgtcgaggtc gacgtcgttc tggtctcggt aggccgggga
     1921 ccgaggaccg acgacgtcgg tctcgatacg gtctcggtca agctcgatag ggggttcgtc
     1981 gaggtcgacc tcaacacgat gcagacggcc cagcccggtc tctacgcggt gggcgacatc
     2041 gtggccggca ccccacagtt ggcccacgtg gggtttgccg aaggaatcgc tgctgtcacc
     2101 catatcggca caggtcgggc ccggccggtc gactatcggg cgatccctcg agtcacctac
     2161 acccatcccg aagtggcgga ggtcggtctt accgagacgg aagctaccga gaaggggctc
     2221 gaggtggcgt ccgcgaagca cgccttgaac ggcatcggta gggcgatcat catcggcgac
     2281 aaccagggtt tcgccaaggt gatctcagag aaggacggcc cgatcgttgg ggcggcagtc
     2341 gtcggtccgc acgccggtga gttgatccac gaactgatgt atgcggtggg atgggaggcc
     2401 ctgcccgatg aggcggcggc tttcatccac gcccacccga gcctgtcgga agtggtcggc
     2461 gagacattga tgtctgccgc cgggcgcagt cttcactgag aggagcgaat ttggccacga
     2521 cggtatcgat gccgcaactg ggtgagacgg tcaccgaggg aaccatcctg cgctgggcga
     2581 agcaggtggg ggacacgatc gccgaggacg aggtcctcgt cgagatctcc accgacaagg
     2641 tcgacaccga ggtcccgtca cctgcggccg gcaccatcct cgagatcctg gtgaacgaag
     2701 gcgacaccgt ctcggtaggc acacccatgg tcgtgattgg tgccgagggt gagggcagtc
     2761 cggcagcaga ggaacaagcg gccgaggcgc tagccgaaga acaaccggct gctccttccg
     2821 gggaagagga gccggccgag ccggcagcgg aggagcaacc ttccgagccg tccgccgatg
     2881 acgagccggt ggagccagcc accgaggaga agccgatcga gcccgaggcc gcttccaccg
     2941 ccgcagagaa gcctgaggag ccgacgccgg cgaaggacga gaaacctacc gagcccgagg
     3001 tcgaggcctt cttcgccaaa cccgacgaga cggagacgaa gccgaacttg ctgtcccccg
     3061 tggtgcgcaa gctgatcagg gaacacgagg tagacgtctc tcaactgtcc ggcaccggag
     3121 acgggggacg gatcactcgc aaggatgtcg aggcctatct ggcgtcgggg ccgagtgtcg
     3181 ccgaggaggc agcgccacag ccggctccga cctccgagcc cgctccgcct gctgccggcg
     3241 agccgcagcc gacgccagaa ccggccgctg cccctcagcc cgccccggtg gcggcagccg
     3301 cagccggtgg ccagggcgcc gacgagatcg agctagaccg gatccgtgtc cggaccgcgg
     3361 agaacatgat caaggcgcgg cagaccgctg cccacgtgtg gacgtcggtg gaggtcgatt
     3421 atgagcgagt ggaacgggtt cggcaggctc acaagcagga attcaaggcg gcggagagtt
     3481 tctcgctcac ttacctccca ttcatcgccc gagccacgat cgacgcccta agggccttcc
     3541 ccgtggtgaa tagctccttc cacctggagg agaagaagtc ggtcatacat cgcaatgtcg
     3601 acctgggaat agcggtcgac ctcgatcagt ccggattggt agtggctcgg gtgcgcaatg
     3661 ccgatggttt gcgcctggtc ggcatcgccc ggtcgatcag ggaactggcg gacaaggcac
     3721 gcaacgggaa gct
//
LOCUS       DASTXO010000259         3532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_311414, whole genome shotgun sequence.
ACCESSION   DASTXO010000259 DASTXO010000000
VERSION     DASTXO010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311414"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..543)
                     /locus_tag="VFU96_05550"
     CDS             complement(94..543)
                     /locus_tag="VFU96_05550"
                     /inference="COORDINATES: protein motif:HMM:NF039728.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HEU5112762.1"
                     /translation="MDETARMEPMGPIEFVLLEFPKRSFDGEIAKALADLADRQIVSI
                     FDLLLVSKDDDGSLEVVELVDAEPTIAERFAEVSGEVMWLLSKDDIAEAAGSLQPNTT
                     GILVVWENTWARHLSSAVARAGGQVVIRDRLDSDAVAASMASSPGSQ"
     gene            complement(601..1299)
                     /locus_tag="VFU96_05555"
     CDS             complement(601..1299)
                     /locus_tag="VFU96_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5112763.1"
                     /translation="MATFVLIPGAGGDPWEWHRLARELEARGHDVVPVQLPAGDDSAG
                     WAEYADAVVEAIGERGEVVLVAQSLAGFSAPLVAERVPIDLIVLLNAMIPIPGETGNA
                     WWSNTRQREAVEAYYASVGLPAETANDEVAVYMHDVPPEIVEEAATHIPEQSMTPMEQ
                     PWPLTSWPDVPTRVLIGGDDRLFPAAFQRRVAKERLGLDGDVIEGGHMVALSRPGDVG
                     DRLEAYWREVDSNR"
     gene            complement(1302..1571)
                     /locus_tag="VFU96_05560"
     CDS             complement(1302..1571)
                     /locus_tag="VFU96_05560"
                     /inference="COORDINATES: protein motif:HMM:NF040451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6510 family protein"
                     /protein_id="HEU5112764.1"
                     /translation="MTNDTHIDGNALGGLFQEVFGRDMTDQAGCCDGCGTVSMLGSVM
                     VFRGAGDVVRCPVCGSTLIVIVSSPGEHRISFESLRWLSVERVSG"
     gene            complement(1613..2806)
                     /locus_tag="VFU96_05565"
     CDS             complement(1613..2806)
                     /locus_tag="VFU96_05565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEU5112765.1"
                     /translation="MATLSSKGRSDVVEIGWPAMANVLVLGGGFGGAQVAIAARELLG
                     DDHRITVVDRADRSLLCGANPLMVVGERPREGVSRSMSTLRQRGVEVVTDEIVAIDLQ
                     RRTADTSRDEFPFDYLVVALGAVYDLDAVPGSSAAHSFYDHAGSVLLREAVERFEGGR
                     VVIGIAGTPYKCPPAPLEAALLLEWHFRRRGLSAEIHLYIPEPAPLAVAGPATSKRVG
                     EMLAARGVVLHTQTTVAVVENMGREVHFSDGASLDADLAVVVPAHRVPAVVTSSGLTR
                     EKPWVPVDPRTLETTAASGVFAVGDVNSVPIGSDRALPKAGVFASAQGRHVAETITSR
                     ILGTSSPAPYTGEGSCFFLLSGEEVAEVGGVFLAPGGPQVGLGEPQPAAVEERWEDNW
                     LRYAI"
     gene            2827..3168
                     /locus_tag="VFU96_05570"
     CDS             2827..3168
                     /locus_tag="VFU96_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013807531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEU5112766.1"
                     /translation="MQIAPPDFDRMFGALADHTRRDIVRRAIDSEEGVAELASHYPMS
                     FAAVQKHVAILERAGLVTKHRIGRRKIVRTNVEGLRVARRLLDQYEELWRGRIDRMTQ
                     LVNEYTRETDQ"
     gene            3165..>3532
                     /locus_tag="VFU96_05575"
     CDS             3165..>3532
                     /locus_tag="VFU96_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003973265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEU5112767.1"
                     /translation="MTVTAVRKDPHNLSMTLDAEFDASPERVWQLWSDPRQLERWWGP
                     PSYPATFTKHDLNPGSRVEYHMTTPEGDQPRGYWDVQEVDPPHSLIVRDGFANDDGSP
                     NTDMPTGEMLVTIEEIGPGRT"
BASE COUNT          633 a         1258 c         1076 g          565 t
ORIGIN      
        1 gacctggctg gatgcgttgg tggtcggggg tggccgtcat cttcacccag aggttttgac
       61 gcatctcacg gacgaccggg gggcggatca gcatcattgg ctccccggcg acgacgccat
      121 cgatgcggcg accgcgtcgg agtcgagccg atcacggatc accacctgcc caccggcgcg
      181 ggcgacggcg ctgctgaggt gtcgcgccca ggtgttctcc cacacgacga gaatcccggt
      241 ggtattcggt tgcagcgatc ctgctgcctc ggcgatgtcg tctttgctca gcagccacat
      301 cacctcaccg ctcacctccg cgaagcgctc ggcgatcgtc ggctccgcgt ctacgagctc
      361 gacgacttcg agactcccgt catcgtcctt gctcaccagg agaaggtcga agatcgagac
      421 gatctgccgg tcggcgagat ccgccaacgc cttggcgatc tcgccatcga acgacctctt
      481 ggggaactcg agcaggacga actcgatcgg gcccatcggc tccatccgcg cagtctcgtc
      541 cattgggacg aatctaccgg cgtggtgcag gggcagacgg cacggcgtgg ttcagaggct
      601 ctaccggttg gagtccactt cgcgccagta ggcctcgagc cggtccccga cgtcacccgg
      661 tcggctcagc gcgaccatgt gcccgccttc gatcacatcg ccatcgagcc ctagtcgctc
      721 tttcgcgacc cgccgctgga acgcagccgg gaacagccga tcgtcgccac cgatcaggac
      781 ccgagtcgga acgtccggcc acgacgtgag cggccagggc tgctccatcg gcgtcatcga
      841 ctgctcgggg atgtgggtcg cggcctcctc cacaatctcc ggtggaacgt catgcatgta
      901 gaccgccacc tcgtcgttgg cggtctcggc cggcagaccg acgctcgcgt agtaggcctc
      961 tacggcctcc cgttgccgcg tgttcgacca ccacgcgttt cccgtctcgc cggggatcgg
     1021 gatcatcgcg ttgaggagca cgatgagatc tatcggcacc cgctccgcca cgagcggtgc
     1081 actgaagccg gcgagtgact gggctacaag cacgacctcg cctctttcac cgatcgcctc
     1141 gaccacggca tcggcgtact cggcccaacc cgcgctgtcg tcacccgccg gcaactggac
     1201 gggcacgacg tcgtggccgc gggcctcgag ctcccgcgcg agtcggtgcc actcccacgg
     1261 atctccgccg gcgcccggga tcaggacaaa tgtcgccatc gtcatccact cacacgctcg
     1321 accgagagcc atcgaaggga ttcgaagctg atccgatgtt cgccgggtga tgagacgatc
     1381 acgatgaggg tgctcccgca caccgggcac cggaccacgt ccccggcccc acgaaacacc
     1441 atgacggagc caagcatgct gaccgtgcca catccgtcgc aacaccccgc ttggtccgtc
     1501 atgtctcggc cgaagacttc ttggaacagc ccgcccaagg cgttgccgtc gatgtgggtg
     1561 tcattcgtca tgatgtcctc ctgggtcccg gtcagcccac cgggctgagc ggtcagatcg
     1621 cataacgcag ccagttgtcc tcccagcgtt cctccacggc cgccggctga ggctcaccca
     1681 gcccgacttg aggccctccg ggggcgagga acactccgcc cacctcggcc acctcctctc
     1741 ccgagagcag gaagaaacag cttccctctc cggtgtacgg cgccggtgag ctagtcccca
     1801 ggatccggga ggtgatcgtc tccgcgacgt gtcgaccctg ggcagatgcg aacacccccg
     1861 ccttgggcaa ggcccgatcg ctcccaatcg ggaccgagtt cacatccccc accgcgaaaa
     1921 ccccggatgc cgccgtggtc tcgagggtcc tcgggtcgac gggaacccat ggcttctcgc
     1981 gagtcagccc agaggaagtg accaccgccg gcacacggtg ggcaggtacc acgaccgcca
     2041 agtcggcgtc gagagacgcc ccatcactga agtggacctc ccgacccatg ttctcgacca
     2101 cagcgaccgt cgtctgcgtg tgaagcacga ctccgcgggc cgccaacatc tcgccaaccc
     2161 gcttcgacgt tgcggggccc gccactgcga gaggagcggg ttccggaatg tagagatgga
     2221 tctcggccga tagcccgcgc cttcgaaagt gccactcgag gaggagcgca gcctccagcg
     2281 gtgccggtgg gcacttgtac ggtgtgccgg cgatgccgat gacgaccctt ccgccctcga
     2341 atcgctccac tgcctctctg aggaggaccg aacctgcgtg gtcgtagaag gagtgggcgg
     2401 ccgaagagcc gggcacggca tccaggtcgt agaccgctcc cagcgcgacc accaggtagt
     2461 cgaaagggaa ttcgtccctg ctggtgtctg ccgttcgccg ctggagatcg atggcgacga
     2521 tctcgtccgt caccacctcg accccacgct gcctcagagt cgacatcgac cggctcaccc
     2581 cttcccgggg ccgctccccg accaccatca gggggttggc gccgcagagg aggctccgat
     2641 ctgcccgatc caccacggtg atgcgatggt cgtccccgag cagttcacgc gcggcgatgg
     2701 ccacctgagc gccgccgaat ccacccccga gaaccaggac gttggccatc gccggccagc
     2761 ctatctccac cacatccgag cgccccttgc ttgacagagt cgccatacaa ccgtatggtt
     2821 gtatgaatgc agatcgcccc acccgacttc gaccgcatgt tcggcgcact ggcggatcac
     2881 acccgccgcg acatcgtgcg ccgagcgatc gactccgagg agggcgtcgc cgagctggca
     2941 agccactacc cgatgagctt cgcggctgtg cagaagcacg tcgccatcct tgaacgggcc
     3001 ggccttgtca ccaagcaccg aatcgggcga cgcaagatcg tccgcaccaa cgtcgagggg
     3061 ctgcgcgtgg cgcgacggct actcgaccag tacgaggagc tgtggcgtgg ccggatcgac
     3121 cggatgactc agctcgtcaa cgagtacacc agggagacag accaatgact gtgaccgccg
     3181 tccgcaagga tcctcacaac ctcagcatga ccctcgacgc cgaattcgac gcctcacccg
     3241 agcgggtgtg gcagttgtgg tcggacccgc ggcagctaga acgctggtgg ggcccgccca
     3301 gctacccggc cacgttcacc aagcacgact tgaatcctgg cagccgtgtc gagtaccaca
     3361 tgaccacgcc cgaaggcgac cagcctcgcg gctactggga cgtccaagag gtcgaccccc
     3421 cgcattcact catcgtccgc gacggattcg ccaacgacga tggctcgcca aacacggata
     3481 tgccgaccgg ggagatgctg gtcacgatcg aggagatcgg cccaggccga ac
//
LOCUS       DASTXO010000260         2724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1379494, whole genome shotgun sequence.
ACCESSION   DASTXO010000260 DASTXO010000000
VERSION     DASTXO010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379494"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..916)
                     /locus_tag="VFU96_05580"
     CDS             complement(152..916)
                     /locus_tag="VFU96_05580"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEU5112768.1"
                     /translation="MPITPADIEKKTFSTALRGYDLDEVDDFLDEMVVAVRELEEELA
                     RAKERVAQLENDPEAMAVAAAAPAPPGPDESAVGRALIAAQAAADRMLEEARTEADQI
                     VGGARSEADTFAMERDQKKAAVDAEMAEMTGLVAGVRNKLAVLATTVADKLDEMDAVV
                     AGAHSRGPIESETDTGTADSDDGLSDDGSADLETFDVESQLVENQGEDQDTGDDSSSV
                     EGAGLEVDLEDLETDHGEDAGSSEIEGEDREENQEI"
     gene            complement(975..1244)
                     /locus_tag="VFU96_05585"
     CDS             complement(975..1244)
                     /locus_tag="VFU96_05585"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU5112769.1"
                     /translation="MGIGGVLILILQIYFYAVLAWVILSWIPTSSEHPLGRVNVFLDR
                     IIYPVILPLRRVIPPLRLGGGMLDLSPIVLLIGIQLLMGLVRGIF"
     gene            complement(1248..1781)
                     /gene="sepF"
                     /locus_tag="VFU96_05590"
     CDS             complement(1248..1781)
                     /gene="sepF"
                     /locus_tag="VFU96_05590"
                     /inference="COORDINATES: protein motif:HMM:NF016363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEU5112770.1"
                     /translation="MSSLFSKALVYLGLVDEDQMDADSNDRPASGPIEEPARRVEPRR
                     VEPFPPSRRVEPPPTARAATRPQGREGLETVGVIRAVETQSDVILVEEFGDARILADR
                     IRDRVPVVLDMRRADNDLVRRVVDFSSGLIYALDGSMRKVGDGLVVVLPPRVTLSRDE
                     KRRLATLGAYELDDIDD"
     gene            complement(1791..2444)
                     /locus_tag="VFU96_05595"
     CDS             complement(1791..2444)
                     /locus_tag="VFU96_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU5112771.1"
                     /translation="MSYEEVVMRMTAAAERAGRDPSSITLVAVSKAKPASDIEAIYEM
                     GHRDFGENRAHEMAEKAARLPEDIRWHFVGGLQTNKARLIRPITHLLHSMDRASLAGA
                     WAKGSGLPPPVLLQINTGHERQKSGVEPEEARSTLAEVLSVGLDVRGLMAIPPLTEDP
                     VAQRPHFAMLRELRSELAGEHPGITELSMGMTDDFELAIEEGSSIIRVGRAIFGERT"
     gene            complement(2441..>2724)
                     /locus_tag="VFU96_05600"
     CDS             complement(2441..>2724)
                     /locus_tag="VFU96_05600"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HEU5112772.1"
                     /translation="MTRQGHEPVAAAIGPGIGPCCFEVGADVAERFPEDVTETRWGAT
                     SVDLAASLRRQLEGLEVWLSGACTMHETGFYSHRRSRTALRNATITWVA"
BASE COUNT          457 a          912 c          840 g          515 t
ORIGIN      
        1 gacgcatgcc gaggagatag gcgccgaggt gctcgcggtt tccatcgagg aggacgtcga
       61 ccaccgggga cagtccttcg acatcgatcg acgctcggtg tcgctcctcg tcgatcggac
      121 gccgagcggc tagctctggg ctccggcccg gtcagatctc ctgattctcc tcgcggtcct
      181 ccccttcgat ctctgacgag ccggcgtcct ccccgtggtc ggtctcgagg tcctcgagat
      241 ccacctcgag gccggcccct tcgaccgagc tcgagtcatc cccggtgtct tgatcctcgc
      301 cttggttttc gaccaattga ctctcgacat cgaaggtctc caagtccgcc gagccatcgt
      361 cggacaggcc gtcatcggaa tcggccgttc ccgtatcggt ttccgactcg atcgggcccc
      421 tgctgtgggc cccggctacg accgcgtcca tctcatcgag tttgtccgcg acagtggtgg
      481 cgagaacagc gagcttgttt cgcactccgg caacgagccc ggtcatctcg gccatctcgg
      541 cgtcgactgc tgctttcttc tggtcgcgct ccatggcgaa ggtgtcggcc tccgatcgtg
      601 cgccgcccac gatctggtcc gcctcggttc gagcctcctc gagcattctg tcggctgccg
      661 cctgggcggc gatgagggcg cgtcccactg ccgattcgtc tggtccgggt ggggcgggcg
      721 cggccgcggc tacagccatc gcctccgggt cgttctcgag ttgggccact cgctccttgg
      781 cccgggcgag ctcctcctcg agctcacgaa ccgcaaccac catctcgtcg aggaaatcat
      841 ccacctcgtc gaggtcgtag ccacggagcg ctgtggagaa ggtcttcttc tcgatgtcgg
      901 caggtgttat cggcatatct atctacccct tggttctggt tgaggatacg aacgaagacg
      961 ccggcctcca aggatcagaa gatcccccgg accaggccca tcagcaattg gatcccgatg
     1021 aggagcacga taggagacag gtcgagcatg ccccctccga gtcgtaaggg cgggatgaca
     1081 cggcgaagtg ggaggatcac cgggtagatg atccgatcga ggaacacgtt caccctgccc
     1141 agggggtgct ccgaggaggt tgggatccag gacaagatca cccacgccag gacggcatag
     1201 aagtagatct ggaggatcag gatcaggacg ccgccgatgc ccactggtca gtcgtcgatg
     1261 tcgtcgagct cgtaagcccc caacgtggcg aggcgccgct tctcatctcg gctcagggtg
     1321 acccgcgggg gcaggacgac cacgagccca tcgcccactt ttcgcatcga cccgtcgagg
     1381 gcgtagatca aacccgacga gaagtcgaca acccggcgga ccaagtcatt gtcggctctt
     1441 cgcatgtcga ggacgacagg tacccggtcg cggatgcgat cggccaggat ccgggcgtcg
     1501 ccgaactcct cgacgaggat cacatcggac tgagtctcga ccgctcgaat cactcccacg
     1561 gtctccaagc cttcacgacc ttggggtctg gtcgcagcgc gtgcggtagg tggcggctcg
     1621 acccggcggc tcggtgggaa tggctccacg cgccgcggct cgacacgccg cgccggctcc
     1681 tcgatggggc cggaagccgg ccggtcgttc gagtcggcat ccatctgatc ctcgtcgacc
     1741 aatcccagat atacgagggc tttggagaac agtgacgaca tatcactccg tcaagtgcgt
     1801 tcaccaaaga tagcccgtcc cacgcggata attgaggagc cttcctcgat cgccagctcg
     1861 aagtcgtcgg tcatccccat cgagagctcg gtgatccctg gatgctcgcc tgccagctcg
     1921 gatcggagct cccgcaacat ggcaaagtgg ggtcgctggg ccacagggtc ctcggtgagc
     1981 ggcggtatcg ccatcaaccc ccggacgtcc agccccacgg aaaggacctc ggccagtgtg
     2041 gatcgtgcct cttcgggctc gactccagac ttctgacgct cgtgcccggt gttgatctgg
     2101 aggagcaccg gtgggggcag gccgctcccc ttcgcccatg ccccggcgag cgacgcccgg
     2161 tccatcgagt gcaggagatg ggtgatgggt cgtatcaacc gggccttgtt ggtctgtagc
     2221 cccccgacga aatgccatcg gatgtcttca ggcagcctcg ccgccttctc ggccatctcg
     2281 tgagctcggt tctctccgaa gtcgcgatgg cccatctcgt agatggcctc gatgtcggat
     2341 gccggcttcg ccttggacac tgccaccaac gtgatcgacg atgggtcccg gccggcgcgc
     2401 tccgccgccg cagtcatccg catcacgacc tcttcgtagc tcatgccacc caggtgatgg
     2461 tggcgttgcg aagcgcggtg cggctccggc ggtggctgta gaaacccgtc tcatgcatgg
     2521 tgcaggcacc ggagagccag acttcgagcc cttccaattg tctcctcaac gatgcggcga
     2581 gatccaccga cgtcgctccc catcgcgtct ccgtgacatc ctcgggaaac cgttcggcga
     2641 cgtcggcccc tacctcgaaa caacagggac cgatacccgg tccgatggcg gcggcaaccg
     2701 gctcatgacc ctgtcgagtc attt
//
LOCUS       DASTXO010000261         3719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1158543, whole genome shotgun sequence.
ACCESSION   DASTXO010000261 DASTXO010000000
VERSION     DASTXO010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158543"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VFU96_05605"
     CDS             <1..295
                     /locus_tag="VFU96_05605"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5112773.1"
                     /translation="TGYGVVGCSRSLPLSAERPEPHPDDPGLRDWRLDAGEGDQAPLH
                     VHHGGEEAFICLDGHLEVEVDGYATTLSRERSSWFLAEPCTPSLLGEARTSSL"
     gene            complement(517..1335)
                     /locus_tag="VFU96_05610"
     CDS             complement(517..1335)
                     /locus_tag="VFU96_05610"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEU5112774.1"
                     /translation="MRRLRRLMVMLAATAISSALFLSAPAGAVIDGTSDTANKYANVG
                     VLQLNVEGEWFDFCSGTLVRADVVLTAAHCTDFLVEEGPDGFGPDDLRISFDPEGDAP
                     FYTVDHIVVHPDWFTAGPCFGNSKHACLAPPAEDIALIFLDESVAGVTPAPIAEEGYL
                     DTLDLKNETFTVVGYGTDEFITGSGASPKAITVFDGIRSYRDVTAIAAQDAFPDRFLK
                     ITAGVCFGDSGGPLFHGETIVALNTWTFSYRCAGPNLEYRVDSAVAQEFLDTYL"
     gene            1590..2228
                     /locus_tag="VFU96_05615"
     CDS             1590..2228
                     /locus_tag="VFU96_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112775.1"
                     /translation="MSRLIVLTLFASALVSCQGSPEVQEAETAPEPTSGGVPWPAPSD
                     PLALAVAAGLEPETHELLDYHVHAHLDVFVNGSPVEVPAGIGIDITDPAVHTTSSDLG
                     PTYGGIDPPCAQPCISPLHTHDSTGVLHTESAVDHPNSLGQFFTEWGVALTPECVGGY
                     CSPDASIQVFVDGEAFSGDPADIELADQLEIAIVIGSPPEEIPSEFPDAAGT"
     gene            3297..3515
                     /locus_tag="VFU96_05620"
     CDS             3297..3515
                     /locus_tag="VFU96_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112776.1"
                     /translation="MASPRVETHGRVVVVRDGEHGEVLAHIEIIDGGFVCKVEPARLS
                     RDAMGATVEVLTTLLLYDLDIEAAAKAK"
BASE COUNT          684 a         1181 c         1210 g          644 t
ORIGIN      
        1 caccggatac ggggtcgtcg gatgctcccg ttccctcccc ctcagcgccg agcgacccga
       61 gccccacccc gacgatccag gattaagaga ctggcgactc gatgcgggcg agggcgatca
      121 agccccgctt cacgtgcatc acggcggcga ggaagcgttc atctgcctcg acggccatct
      181 cgaagttgaa gtcgatgggt acgccacgac gttgagccgg gaacgttcat cgtggttcct
      241 cgcggagccg tgcacacctt cgctactcgg cgaggcgcgc acgtcctcgc tgtgatgagc
      301 cccgagatcg cagaactcat tgacaggctt catgagccga tgagaaccgg tgacgcccag
      361 gctctgtcgg gtcgttacca cccgtcgctc gcctaagccg atctcctgat cgagtgcgag
      421 gaggaccgga cgctccgggc ggtgctcggc atgctacgag agcatgaccg cggacgctaa
      481 ggcgcacccg agtccgcctc agacggccgc gcccgctcag aggtacgtat cgaggaactc
      541 ctgagcgacc gccgaatcga cccgatactc caggttcggc ccggcgcagc ggtagctaaa
      601 cgtccacgtg ttcagcgcaa cgatggtctc gccgtggaaa agtggcccgc cggaatcgcc
      661 gaaacacacc cccgccgtga tcttcaggaa ccggtccgga aaggcgtcct gcgccgcgat
      721 cgcggtcacg tcgcggtagc ttcgaattcc gtcaaagacg gtgatcgcct tcggcgacgc
      781 gccgctaccg gtgatgaatt catcggtccc gtagccgacg acggtgaacg tttcgttctt
      841 caggtcgagc gtgtccagat agccctcctc cgcgatgggc gccggtgtca cgccggcaac
      901 cgactcgtcg aggaagatga gcgcgatgtc ctcggccgga ggcgccaggc acgcatgctt
      961 cgagttgccg aaacagggcc ccgcagtgaa ccagtccggg tgaacgacga tgtggtcgac
     1021 ggtgtagaag ggagcgtccc cctctggatc gaaggagatc ctcaggtcgt cgggcccgaa
     1081 cccatcaggc ccttcctcga cgagaaaatc cgtgcagtgc gcggcggtga gaacgacgtc
     1141 agcgcggaca agcgtccccg agcagaaatc gaaccattcc ccctcgacat tcaactgcag
     1201 cacgccaacg ttcgcgtact tgttggctgt gtcggatgtc ccgtcgatca ccgcgcctgc
     1261 cggcgcagac aagaacaggg cggagctgat ggcggtagcg gcgagcatga ccatcaaacg
     1321 tcgaagtcga cgcatgcttc ctccctctct cctcgatggc gggcgttccg ctcctctcca
     1381 ccgtaagtca gccgtccgcg ctcgcgcaac ggattctttg cccgactggc ctgccagatt
     1441 gtggatcgaa tggcgaggag gaccggacgc tccgggcggt gctcgtcggc gtgctgaggg
     1501 gcgacaagac tcctcccggt agttgcgcgg agagcctgcg ccgcacggcg agtccggcgg
     1561 tgcatactgc ccggatggac gcattgcgcg tgagcagatt gatcgtgctc acgttgttcg
     1621 cttcggcgct ggtctcatgc cagggctcac ccgaggttca ggaagcggag accgcccccg
     1681 agcccacgtc gggtggggtg ccctggccgg cgccatcgga tccgctcgct ctcgccgtcg
     1741 ccgccggcct cgagcccgag acccacgagc tcctcgatta ccacgtccat gcgcatctcg
     1801 acgtcttcgt caacggatca cccgtcgagg tgccagcggg tatcggcatc gacatcacgg
     1861 accctgcagt tcacaccacg tcgagcgacc tcggaccgac gtatgggggc atcgatcctc
     1921 cctgcgcaca gccctgtatc tcgccgctgc acacccatga ctccaccggc gtcttgcaca
     1981 ccgaatccgc ggtcgatcat ccgaactcgc tcgggcagtt cttcacggag tggggcgtgg
     2041 cgctcacccc cgaatgcgtc ggcggctact gttcacccga tgcgtcgatt caggtgttcg
     2101 tggacgggga agcgttcagc ggagatcccg ccgacatcga actggcggac cagctggaga
     2161 tcgcgatcgt gataggttcg ccgcccgagg agatcccttc cgagttcccc gacgccgcgg
     2221 gcacctagtg caggcttgct gtcgccgaat gattggccag gacgatggcg caccgcgagc
     2281 ccgcgccgca cggcgagctg gcacccgagg actcgagccg tactctgtga gtgtcgtggg
     2341 caggcctgag ctatggaagc catgggcgaa cgaccccgag tccttcgaac gtgcggtcct
     2401 cgctgacctc gaacagtcgc cgatgcgatc gaggacgtcc tcggtgacgg ttaacgacgc
     2461 gctggccaca agttggccac aagaaagccc tcctcgggtc caccaggaag gcccttccgc
     2521 aggtagatcg cgtgccctcg acaggaatcg aaggaaacct cgtgagcacg atgatgggga
     2581 gcgaatgaga tgcgtttgcg ctggtagatg cgctgaggtg acctcagcgc ggttggtcaa
     2641 cctacgctgg tggacccaag gtggacccaa actcaacgga ggaactcgat cggctcgtac
     2701 tgggggaccc gactaaccgg accttcggcg agctcctgat cgactgcgag gaggacggga
     2761 cccttagggc ggtgctcgtg gggatgttgc gggaccaccc ttaagggcga ctgccggccg
     2821 gtcgagaatc gcgccatgag gaagtgccca ttctgcgccc agcagaatca gggatgaggc
     2881 gaccgtgtgt cggtactgca accgcgatct cccagagacc ccagcgcaga gagtcacgcg
     2941 ggagcaagcg ggcgaggtcg ggaggctccg gttcgccgcg gaactcatct ggctgatact
     3001 cggcactcta ttcatcgtca atcggcctca cggggcagac ggaacgtggg gcagaggctt
     3061 cggtgatctc ggtcatcatc ggtgtatgcc tggttggctg gagttgctgg cgctggcagg
     3121 tgaaccgagt gaagcgccgc taagtcgccg agctcctgag cgactgcgag gaggtccgga
     3181 cctgcggcgg tgctggtcgg catgctgcgg gaggctgacc gggagcccga gaggttgggc
     3241 ggcagggtta ctgcgacacc tgtcggcaat gtagcctccc ctcagaggtg gtcaacatgg
     3301 caagtccgcg ggtcgagacg cacggtcgtg tcgttgtcgt ccgtgacggt gaacacggcg
     3361 aggtactagc ccacatcgag atcatcgatg gcggcttcgt ctgcaaggtg gagcctgctc
     3421 gtcttagccg ggatgcgatg ggggcaaccg tcgaagtgct gaccacgcta ctgctctacg
     3481 atctggacat cgaagcggcg gcaaaggcaa agtagccaat ccattccgca cctacaaggt
     3541 acgagcaatc agacgatctg ttgcacaaca tcctgaggtc gcagcgaggc gcaagtccag
     3601 cacctcgatg gttgccgact cgttcgaatc cgcatccgtg ctcttcgccg acgtggtgga
     3661 tttcacgtcg atgtcggccg ggatgtcggc cggggacttg gtgaccctgc tcaacgaca
//
LOCUS       DASTXO010000262         3400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1343070, whole genome shotgun sequence.
ACCESSION   DASTXO010000262 DASTXO010000000
VERSION     DASTXO010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343070"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VFU96_05625"
     CDS             complement(<1..540)
                     /locus_tag="VFU96_05625"
                     /inference="COORDINATES: protein motif:HMM:NF025008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEU5112777.1"
                     /translation="MTFAEASAVAAAGDGTWKAEVLPDWDIFGVANGGYLMSIAARAM
                     SEAADGRLPVSLTAHFTRPISAGAVMVSVDPVKEGRTFSTLRATVTAASDSIALLGSF
                     AEPGPGDADDLYVATAPPELPAPEECIRALPSPDGPLPPPLMAQFEERIHPGDTGPLE
                     GRPSGTASMRGWFRLHDGEP"
     gene            complement(537..1511)
                     /gene="purM"
                     /locus_tag="VFU96_05630"
     CDS             complement(537..1511)
                     /gene="purM"
                     /locus_tag="VFU96_05630"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU5112778.1"
                     /translation="MTTYGEAGVDLEGADRHVEAIRSPVTGTWTGDVVGNFGGFAAGV
                     AIPAGYREPVLMMSTDGVGTKLELAKRAGRWDGVGYDLVAMCVDDLTAVGARPLGFVD
                     YLAVGALDPDRDRAIVESVAAACLVAGCPLLGGETAEHPGVMEPDALDLAGAVMGVVE
                     RGEELGPAQVREGDLVVGLVSPNLRSNGFSLIRALFGSEVDSDIDTFLEPSVIYSPDV
                     LHAVKGGGVHAAAHITGGGLAGNLERALPAGAGALIDKRTWEVPSIFDTIASRGAAPT
                     EMWSTFNMGIGFCLVIDPHAGESVIAATSTHQGRIIGKVVSGDGVTLE"
     gene            complement(1513..2937)
                     /gene="purF"
                     /locus_tag="VFU96_05635"
     CDS             complement(1513..2937)
                     /gene="purF"
                     /locus_tag="VFU96_05635"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016189285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU5112779.1"
                     /translation="MEVAVLLTEGPREACGVFAAYTPRQRVSSLIYFGLYGLQHRGQE
                     SAGIATSNGETVTVFKDLGLVSQVFDEPALAGLDGHLGIGHTRYSTTGSNNWANAQPA
                     HRQVGATSIALGHNGNLTNTRELAERLGIRGATTDSDLMAEGIARAIDDERSDQRGLE
                     MAVTEVAPTWEGAFSLVIMDQGRVIGLRDPNGFRPLCLGSLPDGGWVLSSETAALDLV
                     GARFVREVAAGEMVVIDAGGVHSFYPFSATDPKLCLFEFVYFARPDSQMYGRGIHAAR
                     RQMGETLAAEHPALADVVVPVPESGLPAAQGFAAASGIPYADGLVKNRYIGRTFIEPS
                     QSLRDRGIRLKLNPIPDNLRGKRVVLVDDSIVRGSTTRKLVQMVRDAGAIEVHLRVSS
                     PPYRWPCYYGMDTSDRSTLIAASLEVEEIREHIGADSLGYLSLQGLLDATGVADAGFC
                     TACLSGVYPTEVPEAADKFQLERS"
     gene            complement(2976..>3400)
                     /locus_tag="VFU96_05640"
     CDS             complement(2976..>3400)
                     /locus_tag="VFU96_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEU5112780.1"
                     /translation="LFGVVIRAELDRVEVGAYTNIQDNSVLHCDEGIPCVLGERVTVG
                     HAAVVHGALVGDHCLIGIGAKLLNRSRLGEGAWLASGSVLPEGKEIPPWTLGMGIPAR
                     PVRELTDAEVERANDGVDHYQELMERYRSLFRAESATE"
BASE COUNT          584 a         1148 c         1124 g          544 t
ORIGIN      
        1 cggctcccca tcatggagac ggaaccatcc cctcatcgag gcggtgccac tgggcctgcc
       61 ctccagtggc ccggtatccc cggggtggat ccgctcctcg aactgagcca tgagcggagg
      121 tggcaacggc ccgtctggcg acgggagggc gcggatgcac tcctcgggtg cgggtagctc
      181 cggtggtgca gtggcgacat agaggtcatc cgcgtcgccg gggccgggct cggcgaagct
      241 gcccaggagg gcaatcgagt ccgacgcggc ggtcaccgtc gcccgcagcg tggagaacgt
      301 gcgaccctcc ttcaccgggt cgaccgaaac catgaccgcg ccggccgaga tcggcctcgt
      361 gaaatgagcg gtgaggctca ccggtagccg gccatcggca gcctcggaca tggccctggc
      421 cgcgatcgac atcaggtagc cgccgttggc gacaccgaat atgtcccaat cgggcaggac
      481 ctctgccttc cacgttccat ccccggcggc tgcgaccgcc gatgcctcgg caaaggtcat
      541 tcgagcgtca caccgtcgcc ggacacgacc ttcccgatga ttcgcccctg gtgtgtcgaa
      601 gtggcggcga tcaccgattc tccggcatgg ggatcgatga ccaggcagaa gccgatgccc
      661 atgttgaacg tggaccacat ctcggtcggg gcggctccgc gggaggcaat ggtgtcgaag
      721 atcgaaggca cctcccaggt ccgtttgtcg atgagggcgc cggcgcccgc gggaagcgct
      781 ctctccaggt ttccggcgag accacctccg gtgatgtgcg ccgcggcatg gaccccaccc
      841 cccttcaccg cgtgcagcac atccggtgag tagatgaccg atggctccag gaacgtgtcg
      901 atatcggaat ccacttcgct cccgaagagg gcacgaatga gggagaaacc gttcgagcga
      961 aggttgggcg agacgaggcc caccacgagg tctccttccc ggacctgagc gggacccaac
     1021 tcctcaccgc gttcgacgac ccccatcacg gcgccagcga ggtcgagggc gtcgggctcc
     1081 atcacaccgg gatgctcggc ggtctcgcca ccgagcaagg ggcacccggc gacgaggcag
     1141 gcagcggcga ccgattcgac gattgcccga tcgcgatccg ggtcgagggc gcccacggcg
     1201 agatagtcga cgaatcccag gggtctcgcc ccgaccgcgg tgaggtcgtc cacacacatc
     1261 gcgacgaggt cgtagcccac cccgtcccat cgcccggccc gcttcgccag ttccagcttg
     1321 gtccccaccc catcggtcga catcatcaac acgggctctc gatagccggc gggaatggcg
     1381 acgcctgcgg cgaacccgcc gaaattgccg acgacgtccc cggtccaggt gccggtcaca
     1441 gggctcctga tcgcttcgac atgacggtcg gcgccttcca ggtcgacccc ggcctccccg
     1501 taggtggtca tgtcaggagc gctcgagctg gaacttgtct gcagcctccg ggacctcggt
     1561 cgggtacacc ccagagaggc aggcggtgca gaaaccggca tccgcgaccc cggttgcatc
     1621 gagcaggcct tgcagcgaca ggtacccgag ggagtccgcc ccgatgtgct ccctgatctc
     1681 ctcgacctcg agggaggcgg cgatcagagt gctccgatcc gaagtgtcca tcccgtagta
     1741 gcaaggccat cgatagggag gcgaggagac cctgagatgc acctcgatgg cccccgcgtc
     1801 gcgcaccatc tggaccagct tgcgcgttgt ggagccgcgc acgatcgagt catcgacgag
     1861 gaccactcgc tttcccctca ggttgtcggg aatggggttg agcttgagcc tgatccctcg
     1921 atcgcggagg ctctgactgg gctcgatgaa cgtgcgcccg atgtagcggt tcttcacgag
     1981 gccgtcggcg tatgggatgc cactggcagc ggcgaatccc tgtgcggcag gaagccccga
     2041 ctcgggcacc gggaccacca catcggccag cgccgggtgc tcggcggcca atgtctcgcc
     2101 catttgccgt ctcgctgcgt gaatcccccg cccgtacatc tggctgtcgg gtcgagcgaa
     2161 gtagacgaac tcgaacagac acagcttggg gtcggtcgcc gagaacgggt agaagctgtg
     2221 cacccccccg gcgtcgatca ccaccatctc gccggcggcg acctcacgca cgaacctggc
     2281 tcccaccaga tcgagcgcgg ctgtctccga cgagagaacc catccgccgt cgggcaagga
     2341 acccaagcag agtggacgaa agccgttggg gtcacgcagg ccgatcactc gtccctggtc
     2401 catgatcacc aaggagaaag ccccttccca cgtcggagcg acttcggtca cggccatctc
     2461 gagcccccgc tggtcggagc gctcatcgtc gatagcccgg gcgatcccct cggccatgag
     2521 gtcggagtcg gtagtggctc cccggatgcc gaggcgctcg gccaattcgc gggtgttggt
     2581 caggttgccg ttgtgcccga gggcgatgct ggtggccccg acctggcgat gggccggctg
     2641 ggcatttgcc cagttgttgg agccggtggt ggagtatcgg gtgtggccga tgccgagatg
     2701 gccgtcgaga ccggcgaggg cgggctcatc gaagacctgg gagaccaggc ccaggtcctt
     2761 gaacaccgtc accgtctccc cgttcgaagt cgcgatgccg gccgactcct ggcctcggtg
     2821 ctggagcccg tagagaccga agtagatcaa gctggagact cgttgacggg gtgtgtaggc
     2881 ggcgaagacc ccgcacgctt cgcgggggcc ttcggtgagc aggacggcaa cctccatggc
     2941 cacgagccca tcgtagggcg cggcggtcgg tcgacctatt cagtcgccga ttcggctcgg
     3001 aagagggacc ggtatctctc catcagctcc tggtagtggt ccactccatc gttcgccctc
     3061 tcgacctcgg cgtcggtcag ctcccgcaca ggcctcgccg ggatgcccat ccccagagtc
     3121 cacggcggaa tctccttgcc ttctgggagc accgatcccg aggcgagcca ggcgccttcg
     3181 ccgagtcgcg agcggttgag caacttggcg ccgatgccga tgagacagtg gtcgccgacg
     3241 agggcaccat ggacaacggc agcatgaccc acggtcacgc gctccccgag cacgcatggg
     3301 attccctcgt cgcagtggag gaccgagttg tcctggatgt tggtgtaggc cccgacctcg
     3361 actcgatcga gctcggctcg gatcaccacg ccgaagagga
//
LOCUS       DASTXO010000263         2901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1306246, whole genome shotgun sequence.
ACCESSION   DASTXO010000263 DASTXO010000000
VERSION     DASTXO010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306246"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VFU96_05645"
     CDS             <1..302
                     /locus_tag="VFU96_05645"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU5112781.1"
                     /translation="STINNAGRALEVVEERGSLATYFWSWARPEAAPPVEIPSTTEVS
                     TALSRDLKNRGWSFVGPTTVYAFMQAMGLVNDHLDGCHVREECARARRHVLSADL"
     gene            317..1447
                     /locus_tag="VFU96_05650"
     CDS             317..1447
                     /locus_tag="VFU96_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010506798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HEU5112782.1"
                     /translation="MDRPAFTIGIEEEYLVVDRQTRDLVKDPPAEMWDSLREVLGPQV
                     APEFLKAQIEVGTKVCARLAEARNDLAGLRRDLSGVVKEYGAAIIAASTHPCANWTEQ
                     EPTEQPRYQRIAADYQVVARQMVICGMHVHVGIEDEHLRIDLMNQIRYMLPHLLALST
                     SSPFWAGTDTGLLAYRPIIFKNMPRTGAPEEFESWGEYQRTVNILVEAGIIEDATKLW
                     WDIRPSARYPTLEMRISDVCTRLDDAMTVAAFYLCLLGYLFRLRRRNQRWRVYSPFLI
                     SENMWRAQRYGTEGSLIDFGRGELVPFSDLMEEAIEMLAQDALEFDVIEELRHVRTII
                     NEGTSAHRQLAVHKVAVEAGAGEREALEAVVDYLIDDTMYAL"
     gene            1444..1887
                     /locus_tag="VFU96_05655"
     CDS             1444..1887
                     /locus_tag="VFU96_05655"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5112783.1"
                     /translation="MTEYQVDPATLALRISGLLPEDGSHVRQVLFDEIETRLPRDPEA
                     RINIQLSELNPGRTASWHVHNGIVYFVLLRGIVSLQYEGQSEHYSAGDVYTEPIGVVH
                     RAHNPHPEIPAALIGFWVTAAERPHITEVSEPAWEPVEDRHPGLD"
     gene            complement(1896..>2901)
                     /locus_tag="VFU96_05660"
     CDS             complement(1896..>2901)
                     /locus_tag="VFU96_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017982199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5112784.1"
                     /translation="TAAGLGLAVFGVLRSGEWGWIQPKPGGPEWLGISPTVWLILAGA
                     LIIRIFFAWEARVVAGGREPLVRIEMLRNAQLNGGLTMFFFQFMIQAGIFFTVPLFLS
                     VALGLTALETGVRLLPLSVALLLTAVAIPRYRPKAAPRTVVRWGLIALLAGVVSLIAA
                     LEVGVGPEIVAVPMLLTGLGVGALASQLGAVTVSAVPDKETGEVGGLQNTMTNLGASL
                     GTALAGSILIAALTTSFIQGILENPEVPSEVSSAAEVELAGGIPFVSNAQLEAALEDA
                     GVSSSAAEAILSENEQARIAGLRASLSVLALFVVVALYFTRLIPIEPVGTPSGVPAEV
                     "
BASE COUNT          564 a          950 c          907 g          480 t
ORIGIN      
        1 gctcgacgat caacaatgcg ggccgcgccc tcgaggtcgt cgaggagcgc ggctcgctgg
       61 ctacctattt ctggtcgtgg gccaggcccg aagcagcgcc cccggtcgag atcccgtcga
      121 cgaccgaggt gtcgacggcc ctctccaggg atctgaagaa ccggggatgg tccttcgtcg
      181 gcccgaccac cgtctatgcc ttcatgcagg cgatgggcct ggtcaacgat catctcgacg
      241 gatgtcacgt gcgggaagaa tgcgcccggg ccaggcgtca cgtcctgagc gctgacctct
      301 aggtttggac cctgtgatcg accggcccgc gttcacgatc ggaatcgagg aggaatacct
      361 ggtcgtggac cgccaaacca gggatttggt caaggacccg ccggcagaga tgtgggattc
      421 cctgcgcgag gtgctggggc cccaggtcgc acccgagttc ctcaaggcac agatcgaggt
      481 cggaaccaag gtgtgtgcca ggctggccga ggcccgcaac gatctcgccg gccttcgccg
      541 cgacctgtcg ggcgtggtca aggaatacgg agcggcgatc atcgccgcct ccacccaccc
      601 ctgcgccaac tggacggagc aggagccgac cgagcagccc aggtaccagc gaatcgccgc
      661 cgattatcag gtggtcgccc gccagatggt catctgcggc atgcacgtcc atgtcgggat
      721 cgaggacgag cacctgcgga tcgacctgat gaaccagatc cgctacatgc tcccccacct
      781 gctcgccctg agcacgtcct ctccgttctg ggccgggacc gacaccgggc tgctcgccta
      841 ccgcccgatc atcttcaaga acatgcctcg aaccggcgcc cccgaggagt tcgagtcctg
      901 gggcgagtat cagcgaacag tgaacatcct ggtcgaggcg gggatcatcg aggacgccac
      961 caagctgtgg tgggacatac ggccctcggc ccgctatccg accctcgaga tgagaatcag
     1021 cgacgtctgc acgcggctcg acgacgccat gaccgtggcc gccttctacc tttgcctcct
     1081 cgggtatttg ttccgcctgc ggcgtcgaaa tcagagatgg cgggtgtact cgccttttct
     1141 catctcggag aacatgtggc gggcgcagcg ctacgggacc gagggctcac tcatcgattt
     1201 cggacgcgga gagcttgtgc cgttctccga cctcatggaa gaggccatcg agatgctggc
     1261 gcaggacgcc ctcgaattcg acgtgatcga ggagttgcgc cacgtgcgca cgatcatcaa
     1321 cgaagggacc agcgcccatc gccagctcgc ggtccacaag gtcgcggtgg aggcaggggc
     1381 tggtgagcgc gaagcattgg aggcagtggt cgactatctc atcgatgaca ccatgtacgc
     1441 cctatgacgg agtatcaggt cgatccagcg accctcgcac tccgcatttc cggtctgctc
     1501 ccggaagacg gctcgcatgt tcgtcaggtc ctcttcgacg agatcgagac ccgattgcca
     1561 cgtgatccgg aggcacggat caacatccag ttgtccgagt tgaaccccgg ccgaaccgcc
     1621 tcctggcatg tccacaacgg gatcgtctac ttcgtgttgc tgcgcggaat cgtgtcgctc
     1681 caatacgagg gacagagcga gcactattcg gcgggggacg tctacaccga gccaatcgga
     1741 gtcgtgcacc gtgcccataa tccccatcct gagatcccag ccgcgttgat cgggttctgg
     1801 gtgaccgccg ccgagcgtcc tcacatcacc gaggtgtcgg agccggcttg ggagccggtc
     1861 gaagaccggc acccgggtct cgactgaccg aaaggtcaaa cttcggccgg aacccccgat
     1921 ggcgtgccta ccggctcgat cggtatcaag cgggtgaagt acagggccac caccacgaac
     1981 aacgccagca ctgacaaact tgcacggagg ccggctatac gtgcttgttc gttctcgctg
     2041 agaatggcct cggcagcgct cgacgagacg ccggcgtctt cgagcgccgc ctcgagttgt
     2101 gcattcgaca cgaatgggat ccctccggcc agctcgacct cagccgccga cgacacttcg
     2161 gaggggacct ccggattctc caggattccc tggatgaacg atgtggtcag ggccgcaatg
     2221 aggatcgagc cggcgagggc tgtgcccagg gaagcgccca gattggtcat ggtgttctgg
     2281 aggccaccga cttcgcctgt ctctttgtcg ggaactgccg acaccgtgac cgctcccaac
     2341 tgtgaggcca atgcgccgac acccaatccg gtgaggagca tggggactgc gactatctcg
     2401 ggccccactc ccacctcgag agcggcgatc agggatacca ccccggcaag gagtgcgatc
     2461 aatccccacc ggacgaccgt gcggggagcg gccttcggcc gatatctggg gatggcgacc
     2521 gcagtcagca gcagcgccac cgatagaggc agcaatcgca cccctgtctc cagggcggtc
     2581 aggccgagcg cgaccgagag gaagagaggg acggtgaaga agatgcccgc ctgaatcatg
     2641 aactggaaga agaacatcgt caggccccca ttgagctggg cgtttcggag catctcgatc
     2701 cgcaccagag gttcacgccc gcccgccacc accctcgcct cccaagcaaa gaaaatcctg
     2761 atgatgagag ccccggccag gatcaaccac acggtcggcg aaatccccag ccattccggg
     2821 ccaccgggtt tcggttggat ccagccccac tcgccggagc gaagcacgcc gaagacagcc
     2881 agaccgagcc cggccgcggt g
//
LOCUS       DASTXO010000264         2090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_532852, whole genome shotgun sequence.
ACCESSION   DASTXO010000264 DASTXO010000000
VERSION     DASTXO010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532852"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VFU96_05665"
     CDS             complement(<1..180)
                     /locus_tag="VFU96_05665"
                     /inference="COORDINATES: protein motif:HMM:NF013942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase A"
                     /protein_id="HEU5112785.1"
                     /translation="MAKPAVIFGGPSDEHDISILTGLQVTRALGDVHAVYWSKTGDWF
                     EVDTDLEAAEFAEGVP"
     gene            complement(190..1683)
                     /locus_tag="VFU96_05670"
     CDS             complement(190..1683)
                     /locus_tag="VFU96_05670"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU5112786.1"
                     /translation="MIPALLGACVLSAIPAGLRWLRVAQREHYLAGSVATFAGRWWTS
                     GPINSMLLALMVVGLVGSWWSAWWAFLVPVAQLGPIGLSIRGRTSPLAWTARLRRAAI
                     VAGILLLGVFTAGTTLESGFFIALGVFLLPALVDIALLVLGPIERMLGNRWVEQASAR
                     LQSSGAKVVAITGSYGKTSTKQYAAHLLSGTFRTLASPASFNNRMGLARAINEHLVPG
                     TEVFIAEMGTYGPGEIEGLTRWIRPDVSAMVAIGPVHLERFRTLERIVAAKSEILDRA
                     RVGVISVDHPLLKDLALERKETMEVITVSGDGADARVTVEEGLIRIDGVELGAAGLEV
                     FPVNLAVALGIGLALGVEPSAMASRLGGLPHPEHRQALSNSERGFTVIDDTFNSNPAG
                     AAMALGLLERIGNGGRKVVVTPGMVELGPDQFEQNLTFAAESAAVADDLLIVGRTNRD
                     ALLEGSAKGGAAVTVVASREEAVDWVRRNLGPGDAVLYENDLPDHYP"
     gene            complement(1680..>2090)
                     /locus_tag="VFU96_05675"
     CDS             complement(1680..>2090)
                     /locus_tag="VFU96_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005352192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5112787.1"
                     /translation="GAPLVRLAPGRKPSASYRLARFLNRVGLLGDARMEEIRRNRGSA
                     DYRAATGVMREILVKVVNESYEEELGALKSRLIMLWGADDGEVPVAVAERSLRIVRQA
                     GGSAELEVLPGIGHLVPKQAPEAISRVLAEELRN"
BASE COUNT          377 a          723 c          668 g          322 t
ORIGIN      
        1 gggcactcct tcggcgaatt ccgcagcctc cagatcggtg tcgacctcga accaatctcc
       61 cgtcttcgac cagtacacgg cgtgaacgtc gccgagggcc cgggtgacct ggagaccggt
      121 gaggatcgag atgtcgtgct cgtcggaggg gccgccgaag atcaccgccg gcttagccat
      181 tgcgcctcct catggatagt ggtcggggag atcgttctcg tagaggacgg cgtccccagg
      241 gccaagattc cgacggaccc aatcaactgc ctcctcgcga gaggcgacga cggtaaccgc
      301 agcgccgccc ttggcggagc cctccagcaa tgcatcgcga ttggtgcgac cgacgatgag
      361 gagatcgtcg gcgacagccg ccgattcggc ggcaaaggtc aggttctgct cgaactgatc
      421 cggtcccagc tcgaccattc ccggggtgac gacgaccttt cgccctccgt tcccgattcg
      481 ctcgagcaag cccagggcca tcgcggcacc agccgggttc gaattgaagg tgtcgtcgat
      541 gacggtgaac ccccgctccg agttggacag agcctgacgg tgctcggggt ggggaagccc
      601 gcccaaccgc gaagccatgg cgctcggctc tacgccgagg gcgagaccga tccccagagc
      661 aactgccaga ttcaccggaa agacttcgag accggccgcc ccgagctcga cgccgtcgat
      721 ccggatgagc ccctcttcga ccgtcacgcg ggcatccgcc ccatcaccgg agactgtgat
      781 gacttccatc gtctccttcc gctcgagggc caggtctttg aggagcggat ggtcaacgga
      841 gatgacccca acccttgccc ggtcgaggat ctcggacttt gcggcaacga tccgctccag
      901 ggtccggaac cgctcgaggt ggaccggtcc gatggccacc atggccgaca cgtcgggccg
      961 gatccatcga gtgagcccct cgatctcccc tggcccgtag gtccccatct cggcgatgaa
     1021 cacctccgtg ccgggcacca ggtgctcgtt gatggcccgg gccagaccca tccggttgtt
     1081 gaacgaggcc gggctggcca aggtgcgaaa cgttccggag aggaggtggg cagcgtattg
     1141 cttggtgctg gtcttgccgt aggagccggt gatcgccacc accttcgccc cggaggattg
     1201 gagacgtgcc gatgcctgct ccacccatcg atttcccagc attcgctcga ttggcccgag
     1261 tacgagcagg gcaatgtcga cgagcgccgg caggaggaac acaccgagcg caatgaagaa
     1321 cccggactcg agggtggtac cggccgtgaa cacgccaagc aacaggatcc cggcgacgat
     1381 cgctgctcgg cgcagccgcg ccgtccaggc gagcggcgat gtgcggccac gaatcgacag
     1441 gccgatcggg cccagctgtg ccaccgggac gagaaacgcc caccaggcgc tccaccagga
     1501 cccgactaga cccacgacca tcaatgccag gagcatgctg ttgatcggcc cggacgtcca
     1561 ccagcggccg gcgaaggtgg ccaccgagcc cgcgaggtag tgctcgcgtt gcgccacgcg
     1621 cagccagcgc agccctgccg gaatcgcgga caggacacat gcgccgagga gagccgggat
     1681 caatttcgca gctcctcggc cagcacccgg gagatggcct ctggcgcctg cttggggacg
     1741 agatggccga tcccgggcag gacttcgagc tcggcgcttc cccctgcctg cctgacgatg
     1801 cgcagtgacc tctcagccac cgccaccggg acctccccgt catccgctcc ccacagcatg
     1861 atcaagcggg acttgagagc gcccagctcc tcctcgtagc tctcgttgac caccttgacc
     1921 aggatctccc gcatcacgcc ggtcgcggcc cgatagtcgg ccgacccacg atttctgcgg
     1981 atctcctcca tccgggcgtc gccgaggagc ccgacccgat tcaggaatct ggccaagcgg
     2041 tacgaggccg acggcttccg gccgggggcg agtctgacga gaggggcgcc
//
LOCUS       DASTXO010000265         6799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1232580, whole genome shotgun sequence.
ACCESSION   DASTXO010000265 DASTXO010000000
VERSION     DASTXO010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6799
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232580"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFU96_05680"
     CDS             <1..205
                     /locus_tag="VFU96_05680"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEU5112788.1"
                     /translation="IAYDATVGPIGGIRQKVVGAEAVGAEYILVPADNYESALTAPRE
                     SIEIVPIATLQDAIDFFEDLGQA"
     gene            262..2061
                     /locus_tag="VFU96_05685"
     CDS             262..2061
                     /locus_tag="VFU96_05685"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEU5112789.1"
                     /translation="MASDITPDEIRRAEFRAVRRGLDPDEVSSFLERVAADVGKLQEE
                     RERLATKLGEFADRDLESEFETLGREVAAVLQSAKEAADSMRERATMDATRWRSEAMT
                     EVETTRKEARNDAEALRGDAWATGTELLNQSAAEARRIRSEAEQDVLSVMGEAEREAH
                     RLTSTSRREAEDVLRSAMMDSEKLASEAKKRHDDVIEQAYRQAESAQERTRALEERRE
                     ELMLELETVRSTLSRLEGTLEERREDLELSSESTSVKVVPARTEPDEKTWELGETVRV
                     IPPFERPPSEPPGPPLERPRPAVRVVESEPPADILDEPIIESAAPPPEPLREEIEQRR
                     PEPEPEPDPESEPGPEPEPEVRPDDVGALFASLRGSPQATITVHTAATPEVEEVGAED
                     ASQQGVAAPSEFIEERDARLLPITNRALRGVKKAVTDAQNVALDSLRIDDSCHPDSQV
                     LAEMMQADLKGLWAESYSAGHAAAEEMTGAKLKRRDTPPSKAADTFGEDLAGAVSHAL
                     IDAGDGQREKQSATSRVFRGWRTDEAERRVRQLALVGYHRGLVDAVGEEDSLQWVASG
                     TPCSACREAATEPGSHLPPVHAGCECTVVLAGV"
     gene            2112..5021
                     /locus_tag="VFU96_05690"
     CDS             2112..5021
                     /locus_tag="VFU96_05690"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEU5112790.1"
                     /translation="MFTRVPADYRADRRRPITLILIVLAALYLGLTALGTLWTDYLWL
                     GSIGFEGVWQRRLGLSILLGALGIAVAFLVLWVNLRLVDRMSPRWAPFDLTEEEEVIE
                     RFREWVEPRIRQIRFWLTGGLAVLFGLTVASWRDEVFLFINAQEFGTNDPIFTLDVGF
                     YVFRLPLLETATDWVFNLLVLATVVTVIGHYFNGGLRLSGRRLTATRRAKTHISVLLA
                     LIALVRAVVYRIDMYELLLSDRVAEQFFGPGFTDITARLPAYRLLILVAVLAAILFIV
                     NIFRPGWTLALVAVGSWLVVAIAAAAIYPAIIQRLQVNPQPQAREGPYIENSIKFTRE
                     AWGLDEVEVRGFPASDDLEVADIEANQLTIDNLRLWDPSVLPRTYQSFQELRNYYTLS
                     VVDTDRYLNQGTPIQVMLAVRELEEVNLPREDWLNQRLLYTHGFGAVVNVANEVADDG
                     QPNFLLKDVPPQAAVEGLALDEPRVYFGETYQEGRPVIVKTGDEPQEIDFPLADEQTA
                     YNEYAGDAGVTLDNIWKRIAFAFRYRDLNLLISGEVRPDSRVLVERNVSHIVESVAPF
                     LHLDDDPYPVIENGEILWVVDLYTTSSWYPYSQPLEPEAIGRLARSSDLRPGINYMRN
                     SVKAVVDAHDGHVTLYLVDPEDPVASAWDQAYPGLFTPFSEMPDSLVEHLRYPQDLFR
                     VQGQLYLEYHVTDPNELFSGNDAWSLPADPATINRDPASLGAELLFGDTRDQTTGQLN
                     YRREILPYYLLTKLPGEEDLSYLLLQPFTPRDRRNMSAFLVADSTPGRYGRLVDFRMP
                     QGQLVDGTEQVGQRIDQDAEISEQFTLWDNQGSVVIKGDLLVVPIEDSILYIQPIFLE
                     AEGGGIPEFRRVIVVYRDQVEWSETLDGALEQVFGDGTEPTEPSEPTEPGPDGGTVEE
                     LLNQAAEAFAAADQALTAGDLAEYQRWVDEAERLIEEAQAIMADAVEAARRAPAS"
     gene            5103..5330
                     /gene="infA"
                     /locus_tag="VFU96_05695"
     CDS             5103..5330
                     /gene="infA"
                     /locus_tag="VFU96_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU5112791.1"
                     /translation="MAKDEDEYIRMRGTVLKMLPDSTFEVQLEGGLELLAKPSGRMRR
                     GRGIRVLPGDTVDVEVSTYDPTRGRIVWRYR"
     gene            5364..5501
                     /locus_tag="VFU96_05700"
     CDS             5364..5501
                     /locus_tag="VFU96_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112792.1"
                     /translation="MESVRVLRHDAVMELAMNLVAGAAAVLMILVGGYAIAAIWWDDL
                     S"
     gene            5532..5897
                     /locus_tag="VFU96_05705"
     CDS             5532..5897
                     /locus_tag="VFU96_05705"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5112793.1"
                     /translation="MRLSNIILRVADLEESVAFWRDLVGLDLSWAGDEFAFFAVGDNQ
                     LTLNQPLVFEDQASDTEIVFEVEDVHSAISEMRDRGVPFEVEPRAVTTDGERTLYAAH
                     FRDPDGHVASVTGWVEGTE"
     gene            complement(5973..6131)
                     /locus_tag="VFU96_05710"
     CDS             complement(5973..6131)
                     /locus_tag="VFU96_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112794.1"
                     /translation="MDRRLRKTKAIAAAITLALAVAACDSDDTDPTDPADGGVTTTLP
                     AETTTAAG"
     gene            complement(6247..>6799)
                     /locus_tag="VFU96_05715"
     CDS             complement(6247..>6799)
                     /locus_tag="VFU96_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5112795.1"
                     /translation="EEIKAMVTTTIETYGGLHVAVNNAGIGGAQANTGDYPIDSWQQV
                     IEVNLGGVFYCMRHEIPAMLASGGGAIVNMASILGSVGFASSPAYVAAKHGVVGLTKS
                     AAIEYSAQGIRVNSVGPGFINTPLLSENLDAETLEVIAGMHPIGRLGESQEVADLVAF
                     LGSDEASFCTGGYYLVDGAYTAN"
BASE COUNT         1236 a         2194 c         2160 g         1209 t
ORIGIN      
        1 catcgcctac gacgcaaccg ttgggcccat tggaggtatt cgtcagaagg tcgtcggagc
       61 agaagcagtc ggtgctgagt acatcctggt cccagcggac aactacgagt ccgccctcac
      121 ggcaccccgc gagtcgatcg agatcgttcc gatcgccacg ttgcaggatg cgatcgactt
      181 cttcgaggac ctgggccagg cctgactcgt tctcgcccaa acccgggcga aatccggccc
      241 gatcggtatc ttctagtgcc gatggcttct gacatcaccc ccgacgagat ccggcgggcg
      301 gagtttcgtg ccgttcggcg tggcctcgac cccgatgagg tttccagctt cctcgagcgg
      361 gtcgccgccg acgtgggcaa gctccaggag gaacgagagc ggctggcgac caagcttggc
      421 gaatttgccg atcgagacct ggaatcagag ttcgagacct tgggtcgcga ggtggctgcg
      481 gtattgcagt cggccaaaga ggccgcagac tcgatgagag agcgggccac catggacgca
      541 acgcgctggc ggtccgaggc catgaccgaa gtcgaaacga cgcgcaagga ggcccggaat
      601 gacgccgagg cccttcgggg cgacgcgtgg gccaccggca ccgaattgct caatcagagc
      661 gcggctgaag cgcgtcgcat acgcagcgag gcagagcaag acgtgctgtc ggtcatgggg
      721 gaggcagagc gcgaggccca ccgtctgact tcgacttctc ggcgcgaggc cgaggacgtt
      781 cttcggtccg cgatgatgga ttccgagaaa ctggcgtcgg aggcgaaaaa gcggcacgac
      841 gacgtgatcg agcaggccta tcgccaggct gagagtgcac aagagcggac ccgtgccctc
      901 gaggagcgac gcgaggagct gatgctcgag ctcgagacgg tccgctcgac tctgagccgt
      961 ctcgaaggaa ctctcgagga gcgtcgtgag gacctcgagc tctcatcgga atcgacgagc
     1021 gtcaaggtcg tccccgcccg aaccgagccg gacgagaaaa cctgggagct gggagagacg
     1081 gttcgagtga tccctccttt cgagaggcca cccagcgagc cacctggccc tcccctcgag
     1141 cggccccgcc cggcggtgag agtggtggag tcggagcctc cagccgacat cctcgacgag
     1201 ccgatcatcg aatcagcggc tccgccaccc gaaccgctgc gcgaggagat cgagcagcgc
     1261 cggcctgagc cggagcccga gccggatcca gagtcggagc ccgggccgga accagaaccg
     1321 gaggtgcgcc ccgacgatgt aggcgccctg ttcgcctcac tccggggctc gccacaagcg
     1381 acgatcaccg tgcacacagc agcgacccct gaagtcgaag aagtcggggc agaggatgcc
     1441 tcgcagcagg gcgtcgccgc gcccagcgag ttcatcgagg agcgggatgc ccggcttctc
     1501 ccgataacca accgtgcgtt gaggggcgtg aagaaggcgg tcacggacgc tcagaacgtg
     1561 gccctcgaca gcctgcgcat cgatgacagt tgtcacccgg acagccaggt cctggccgag
     1621 atgatgcagg ccgacctcaa gggtctctgg gccgagagct actcggcggg tcacgctgcg
     1681 gcggaagaga tgacgggcgc caagctgaag cggagggaca ccccaccctc caaggccgcc
     1741 gacacctttg gcgaggacct ggccggcgcg gtctcccatg ctctcatcga cgccggagat
     1801 ggacagcggg agaagcagtc cgcgacatcg agagtgttcc ggggctggcg caccgacgag
     1861 gccgagcgca gagtgcgcca actcgccctg gtcggatacc accgcggctt ggtcgatgcg
     1921 gtgggtgagg aggacagcct ccagtgggta gccagcggca ctccatgcag cgcctgtcgc
     1981 gaggcagcga ctgagcccgg gtcacacctg cccccggtgc atgccggctg cgaatgcacg
     2041 gtcgtgctcg ccggcgtgta gacagaccga gcacgaaacc aagcacggcg gagccgttac
     2101 gctcatcgcc aatgttcacg agggttccag ccgactatcg ggcggacaga cggcggccaa
     2161 tcacattgat cttgattgtg ttggcggcgc tgtatctcgg cctgaccgcc ctgggaacgc
     2221 tctggaccga ctacctctgg ctcggttcga tcggcttcga gggggtttgg caacgccgat
     2281 tgggcctttc gattctgctc ggcgccctgg gcatcgctgt cgccttcctc gtcctctggg
     2341 tcaacctccg cctggtcgac cgaatgtcac cccgctgggc acccttcgac ctgaccgagg
     2401 aagaggaggt gatcgagcga ttccgcgagt gggtcgagcc ccggatccga cagatccggt
     2461 tctggctgac cggtggactg gcggttctct tcggcctgac cgtggcctcg tggcgagacg
     2521 aagtgtttct cttcatcaat gcccaggaat tcggtaccaa cgatccgatc ttcaccctcg
     2581 acgtcgggtt ctacgtattc cgtcttccgt tgctggagac agcaacggac tgggtgttca
     2641 acctgctcgt gttggccact gtggtgacgg tcatcggcca ctacttcaac ggtggtctgc
     2701 gtctgagtgg ccggcggctg acggcgactc ggcgggcgaa gacccacatc tcggtgttgc
     2761 tggccctgat tgccttggtg cgcgcggtgg tctaccgcat cgacatgtac gagctcctct
     2821 tgtcggatcg ggtagcagaa cagttcttcg gccccggttt cacggacata accgcccgct
     2881 tgcctgctta tcgcctcctc atcctggtcg cggtcctggc ggcaattctc ttcatcgtca
     2941 acatcttccg gcccgggtgg acactggccc tcgtcgcggt cggctcctgg ctcgtggtgg
     3001 cgatcgcagc tgccgcgatc tatcccgcca tcatccagcg tctccaggtc aacccccagc
     3061 cgcaggcgcg ggaaggccca tacatcgaga acagcatcaa gttcacccgg gaggcatggg
     3121 ggttggacga ggtcgaggtg cggggttttc ccgcctccga cgatctcgag gtggcggata
     3181 tcgaggcgaa ccagctgacg atcgacaacc ttcggctctg ggatccctcg gttctgccac
     3241 gcacctacca gagctttcag gagcttcgca actattacac cctgtccgtg gtggatacgg
     3301 accgatacct caaccaaggc actcccattc aggtcatgct ggcggtgcgg gagctcgagg
     3361 aagtgaatct gccccgtgag gactggctga accagcggct tctctatacc cacggcttcg
     3421 gggctgtggt caatgtggcc aatgaagtgg ccgacgacgg gcagccgaac ttcctgttga
     3481 aagacgtgcc gcctcaggcg gccgtcgaag ggctggcgct cgatgagccc agggtctatt
     3541 tcggggagac ctaccaggag ggaagaccgg tcatcgtgaa gaccggcgat gagccgcagg
     3601 agatcgactt tcccctcgct gacgaacaga ccgcgtacaa cgagtacgcg ggcgatgccg
     3661 gtgtgacact ggacaacatc tggaagcgaa tcgccttcgc cttccgctac cgcgacctga
     3721 atctgctcat ctctggcgag gtgcgcccgg acagcagggt gctcgtcgag cgaaacgtga
     3781 gccacatcgt cgagagcgtc gcccctttcc tccacctcga cgacgacccc tatcccgtca
     3841 tcgagaacgg cgagatcctg tgggtggtcg atctctacac gacctcgtcc tggtacccct
     3901 actcgcagcc gctggagccc gaggccatcg gccggctggc ccgatcctcc gatctccgac
     3961 ccgggatcaa ctacatgcgc aactcggtca aggcggtagt cgacgcccat gatggccatg
     4021 tcaccctcta tctggtcgat cccgaagacc ccgtggccag cgcatgggac caggcctatc
     4081 ccggtctctt caccccgttc agcgagatgc ccgacagcct cgtagagcat ctccgctacc
     4141 cgcaggatct gttccgggtg cagggtcagc tctatctcga gtaccacgtg accgatccca
     4201 acgagctctt cagtggcaac gacgcctggt cgctgccggc cgacccggcc acgatcaacc
     4261 gtgacccggc ttcactcggc gcggagctgc tcttcgggga tacccgggac cagacgaccg
     4321 ggcagttgaa ctatcggagg gagatcctgc cctactactt gttgacaaag cttccaggtg
     4381 aggaggatct ctcctacctg ttgctccaac cgttcactcc ccgcgacagg cgcaacatgt
     4441 cggcattcct ggtcgccgac tccactccgg gtcgctacgg ccggctcgtc gacttccgga
     4501 tgccccaggg tcagctcgtc gacgggaccg agcaagtcgg tcagcgaatc gatcaggacg
     4561 cagagatctc tgagcagttc actttgtggg acaaccaggg ctcggtggtg atcaagggtg
     4621 atctcctcgt ggtcccgatc gaggattcga tcctctacat ccagccgatc ttcctcgagg
     4681 cggaaggggg cggcatcccc gagttccggc gagtgatcgt tgtctaccgc gaccaggtcg
     4741 agtggtccga gaccctcgat ggtgcgctgg agcaggtgtt tggtgacggc accgagccga
     4801 ccgaaccgag cgaaccgacg gagccaggcc cggatggggg caccgtcgag gagctgttga
     4861 accaggcggc cgaggcgttt gccgctgcag accaagccct caccgccggc gatctggccg
     4921 agtatcagcg ctgggtcgac gaagccgagc gtctgatcga ggaggcccag gccatcatgg
     4981 ccgatgcggt cgaggcggcg agaagagcac cggcctcata ggaacacgag cgtctcacca
     5041 atctgggcat tccctacacg cccccgagcc ctcctacact ggccacctcc ttcaggaaga
     5101 acatggcgaa agacgaagac gaatacatac ggatgcgggg cacagtgctc aagatgctgc
     5161 ccgactcgac cttcgaggtg cagttggaag gtggcttgga gctcctggcc aagccctcgg
     5221 gccggatgcg gcgcgggcgt ggcatccgcg tcctccccgg tgacaccgtc gacgtcgagg
     5281 tatcgaccta cgaccccacc cgaggtcgca tcgtctggcg gtaccgctag aactgccaag
     5341 cggcgcaacg ggtcgctcgc attatggaat ctgtcagggt cctccggcat gatgccgtca
     5401 tggagctggc catgaatttg gtggcggggg ctgccgcggt tttgatgatc ctggtcgggg
     5461 gctacgccat cgccgccatc tggtgggacg acctgagctg aggtctagct gccgggagta
     5521 ggttccccgg gatgcgtctc agcaacatca tccttcgagt cgccgacctc gaggagtcgg
     5581 tggcgttctg gcgcgacctg gtcgggctcg acctgagttg ggccggagac gagttcgcct
     5641 tcttcgcagt cggcgacaac cagctcaccc tcaaccagcc gttggtcttc gaggatcagg
     5701 cgtcggacac cgagatcgtg ttcgaagtcg aggatgtgca ctccgccatc tcagagatgc
     5761 gcgatcgggg tgtccctttc gaagtcgagc cgcgagcagt gaccaccgac ggtgagcgca
     5821 ccctctatgc cgcccatttt cgggatccgg atgggcacgt cgccagtgtc accgggtggg
     5881 tcgagggaac ggagtagccc ggctccttca acggtcatga acgcgccgga gggcgccacc
     5941 gggatggcgc cctccaacag ccaccggcgg cgtcagccgg cagcagttgt ggtttcggcc
     6001 ggcagcgtgg ttgtcacgcc gccatccgcc gggtcggtcg ggtcggtgtc gtcgctgtca
     6061 caggcagcaa ccgccaacgc cagtgttatc gcggctgcga tcgccttggt cttgcgcaat
     6121 ctccggtcca tatgtcctcc attcaaggga cacgtgtcgc ccggcccgcc ggacgcccac
     6181 gcgacaagca acgctcttat cccttcggac caagtcctaa accgcctttt tcgggtttct
     6241 gccgcctcag ttggcggtgt aggcaccatc gaccaggtag tagccgcccg tgcagaacga
     6301 agcctcatcc gacccgagaa aagccaccag atcggccacc tcttgggact cccccaaccg
     6361 accgatcggg tgcatcccag ctatcacctc gagagtttcc gcatcgaggt tctccgacaa
     6421 caacggtgtg ttgatgaaac cagggccaac cgaattcacc cttattccct gggccgaata
     6481 ctcgatcgcg gcgctcttgg tcagaccgac cacgccgtgt ttcgccgcca catatgccgg
     6541 tgagctggcg aatccgacac ttcccaggat cgacgccatg ttgacgatgg cccctcctcc
     6601 agaagcgagc atcgccggga tctcgtgtct catgcaatag aagaccccgc ccaggttgac
     6661 ctctatgact tgctgccagc tgtcgatggg gtagtccccg gtgttggcct gagcgccgcc
     6721 tatgccggcg ttgttgaccg cgacatgcag accgccgtag gtctcgatcg tcgtggtcac
     6781 catcgccttg atctcctcg
//
LOCUS       DASTXO010000266         4625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_496037, whole genome shotgun sequence.
ACCESSION   DASTXO010000266 DASTXO010000000
VERSION     DASTXO010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496037"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /locus_tag="VFU96_05720"
     CDS             complement(<1..1103)
                     /locus_tag="VFU96_05720"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU5112796.1"
                     /translation="MAVFVMAVVAVPSFSGTTGKLSGKVVSEKNEPLAGVNVRVEGQR
                     VGAITDDKGDYAIIGVLAGTYVVRANLLGYAAFAAQNVTIQPDFTTELNITLKSEAVQ
                     MEEVRVDAERPLLQKDATGTTRFITSDDMQKLPTRGYRDAAAQQTGVVNFQLNIDNEA
                     QNAPTLIVRGGRPNETAYFVDGFSQQDPLTGTSSTSISNNAIEEVVLLTGGFSPEYGR
                     IMSGAVNVITREGGAKYSGALEAVSDAIAGDWISAPVTDYNIYDVSLGGPLLPGYDKV
                     TFYVSGERRWQRDRAPSHLPGQFQDALADLSLPNEFKPHNSSSGGTFQAKLAWQMTEQ
                     DQIKVGTLSSNEQWSQYLHSYLYNLDHAPRYED"
     gene            complement(1494..2747)
                     /locus_tag="VFU96_05725"
     CDS             complement(1494..2747)
                     /locus_tag="VFU96_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112797.1"
                     /translation="MHSRIVRLIVLGVILLMALPVAALAQTSRVEGMALQGDYIKDYT
                     GIYGYPSQIGNVGNLIYGELGVAVGGVPVDRSVGTVMGDWWEGRFGTFGVHLRQITPQ
                     FGQGDATSSPNPGIFGSDPNTNTQEQFDLMWGRKMGTANFGLRFHRSNGQVEIGNNFL
                     GLGNISSLKFDFTQLDPNLSRNVMGLGVGLGWEMSPSTNLEVAGHYQSRSFELTDTLG
                     TTIAEDDGKTTYQLLARAFWQWQPNVMVVPVFKWYSYDLSTATGPTIASNHQNGWQLG
                     AAGNWTIGSNDLFVLGATFAQNRIEHEADIFAVGFANGEISETFTPQLFAALETHVNS
                     WLTLRFGANKGAWHSVEVTDNSTPGTLTIHDSPFAMSLGAGVKVGGLQLDAVMNDSFP
                     HNGLYFVSGASTSPLFSKVTATYSF"
     gene            complement(3053..3892)
                     /locus_tag="VFU96_05730"
     CDS             complement(3053..3892)
                     /locus_tag="VFU96_05730"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEU5112798.1"
                     /translation="MKTRDSEAKGKLAIVNIAALDLRRLPDHRAELRSQLLLGEVVRC
                     LASDRTRQWVRVENRVDHYRGWVRSWGLMPVTAPEAASWLEGGRLRVVAHHAEVRASA
                     GGRLLISPVFFNTRLAPIGTRKRSGSAWRPVRLPDGRSGWIPKAAVRRSVGRSPRLAL
                     QVGSFLGVPYLWGGRTPLGFDCSGFVQQLLASRGIRIPRDADQQYRSSQRLGGRDRGR
                     SGDLLFFGPPRGPVTHVGVHIGSTLFAHARGWVRINSVDPRNRLYDKALMASLRAVCR
                     PLP"
     gene            complement(3895..>4625)
                     /locus_tag="VFU96_05735"
     CDS             complement(3895..>4625)
                     /locus_tag="VFU96_05735"
                     /inference="COORDINATES: protein motif:HMM:NF040439.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HEU5112799.1"
                     /translation="SFRVKDTRSYGGDVQWLAARARLFDRRDGSWPREYRFGYRIVDD
                     VGAQIIRGDTRVTVKRSADPVIIRPTESDLFVGRYAFEVELTEGRSRWKVDRTFEVEE
                     SGPPRGAALEKLLEPLSYISRPEEIRGIRDADSTLQVARWEAFWARRDPTPDTPRNEA
                     LIEFIRRVRHAERHFQGYGPGWRSDMGRIYIRYGPPDQVESRAAGSDTPQLEIWYYHN
                     PVRTFVFGDREGFGRYTLMSPIGE"
BASE COUNT          844 a         1482 c         1472 g          827 t
ORIGIN      
        1 tggtcctcgt agcgcggggc atgatcaagg ttgtacaggt agctgtggag atactgactc
       61 cattgctcgt tcgacgacag cgtcccgacc ttgatctgat cctgttccgt catctgccac
      121 gcgagtttgg cctggaacgt cccgcccgag gagctgttgt ggggcttgaa ctcgttcggg
      181 agactcaggt ccgccagcgc gtcctggaac tggcccggaa ggtgcgacgg cgcccgatca
      241 cgctgccagc ggcgctcccc ggagacgtag aaggtgacct tgtcgtagcc cgggagcagc
      301 ggcccaccaa gtgagacatc gtagatgttg taatcggtca ccggtgcgct gatccagtcg
      361 ccggcgatgg cgtccgagac ggcttcgagc gcgccggagt acttggcgcc gccttcgcgc
      421 gtaatcacgt tcacggcgcc ggacatgatg cgtccgtact cgggcgagaa tccgccggtg
      481 agcagcacga cctcctcgat cgcgttgttg ctgatcgagg tgctcgacgt gccggtcagc
      541 ggatcctgct gagaaaagcc gtcgacgaag tacgcggtct cattgggccg tccgccgcgc
      601 acgatcagcg tcggggcgtt ctgagcctcg ttgtcgatgt tgagctggaa gttgaccacg
      661 ccggtctgct gggccgccgc atcgcggtag ccacgcgtgg gcagcttctg catgtcatcc
      721 gaggtgatga agcgcgtggt gccggtggcg tccttctgaa ggagcgggcg ctcggcgtcc
      781 acccgtacct cctccatctg taccgcctcg gatttgagtg tgatgttgag ctcggtggtg
      841 aagtcgggct ggatggtcac gttctgggcc gcgaaggcgg catagcccag aagattggcc
      901 cgcactacgt aggtcccggc caggaccccg atgatcgcgt agtcgccctt atcgtcggtg
      961 atggcaccga cgcgttggcc ttcgacgcga acattgacac cggcgagcgg ctcgttcttc
     1021 tcgctcacga cttttccgga caacttgccg gtcgtgcccg agaaggatgg gaccgccacg
     1081 acggccatga cgaacacggc cacgaccact gcccagggag ctttgcggag catgggctga
     1141 tctcctttcg agaacaaaat ttgcggaacg tcgcgtggtg gacgacgatc cgatcggcct
     1201 ttttggggag gaacttaact taaggaggga gttgtttaga ccatgtcacg ctctcctgtt
     1261 cccctttcat gcggtgggag gggaacgcct tccgttggcc tggagtgctg ggggatgaga
     1321 cccacgcgcg aaccgtagaa ccaagtgcag agaactgtca acgcaaaaca gacggcgtca
     1381 actcgagggc tgactgcaac cgactcgggc tcagccgatt gagctggcgg gtgcgccaaa
     1441 aagacgaacc ggggagccca agagggctcc ccggtttcac ttggaccagg cgactagaac
     1501 gaatacgtcg ccgtgacttt cgagaacagc ggcgacgtgc tggcgccgct cacgaagtag
     1561 agaccgttgt gcgggaacga gtcgttcatg accgcgtcga gctgcagacc gcccaccttg
     1621 actcccgcgc ccagcgacat cgcgaacggc gagtcgtgga tcgtgagcgt ccccggcgtg
     1681 ctgttatcgg tgacctcgac ggaatgccag gcgcccttat tggcgccgaa acgcagtgtg
     1741 agccagctgt tgacgtgggt ctcgagggcc gcaaataact gcggtgtaaa ggtctcgctg
     1801 atctcaccgt tggcgaatcc gacggcgaag atgtcggcct cgtgctcgat cctgttctgc
     1861 gcgaaggtgg cgcccaggac gaacagatcg ttggagccga tcgtccagtt gccggcggcg
     1921 cccagctgcc agccgttctg gtggttgctg gcgatggtgg gtccggtcgc cgtcgagagg
     1981 tcgtaggagt accacttgaa caccggcacc accatcacgt tcggctgcca ctgccagaag
     2041 gcccgggcga gcagctgata ggtggtcttc ccgtcatcct cggcgatcgt ggtgccgagg
     2101 gtgtcggtga gctcgaatga ccggctctgg tagtggccgg ccacctccag gttggtgctg
     2161 gggctcatct cccagcccag gccgacgccc agacccatga cattgcggga caggtttgga
     2221 tccagctggg tgaagtcgaa cttcagactg ctgatgttgc ccaggcccag aaagttgttg
     2281 ccaatctcga cttgcccgtt cgagcggtgg aaccggaggc caaagttggc ggtccccatc
     2341 ttgcggcccc acatgaggtc gaactgctcc tgggtgttgg tattggggtc cgagccgaag
     2401 atgcccgggt tgggcgagga cgtcgcgtct ccctgtccga actgcggcgt gatctggcgg
     2461 aggtgaacac cgaaagtacc gaaccgcccc tcccaccagt cacccatcac ggtcccgacc
     2521 gagcggtcaa ccgggacgcc tcccacagcg acgcccagct cgccatagat gaggttcccg
     2581 acgttgccaa tctgtgaggg gtacccgtag ataccggtgt aatccttgat gtagtcgccc
     2641 tgcagggcca tgccctcgac gcgcgaggtc tgggccaggg ccgcgaccgg cagcgccatg
     2701 agcagaatca cgcccaggac gatcaacctg acgattcgac tatgcatgag agaccgcctc
     2761 cgttttcggt ttaagtagag ctacacaacg aagccacgat tgcagatcaa gccgttgaac
     2821 gtggcctgcc cactacaacg cggtttcagt cgaagcccca ccccctttcc ctagccgtct
     2881 cacgagttac gcgagcatga caaaggctcc atcgcctccc tgtgatggag cactcagaaa
     2941 aaaagccagg aactacctcc acagttgttt aggaggctcc tggcaaacgc ccgatggagg
     3001 gtagtgtccg caacgaaaac ctgtcaacag caatgtcggg gtttgttgcc catcagggca
     3061 gtggcctgca gacggcgcgg agcgacgcca tcaaggcctt gtcgtacagt cggttgcgtg
     3121 gatcgacact gttgatccgg acccagccgc gcgcgtgtgc aaacagggtc gagccgatgt
     3181 ggactcccac atgggtcacg ggaccacgcg gcggtccaaa gaagagcagg tcgccgctcc
     3241 tgccccgatc tctcccaccc aatcgctggc tcgaccggta ctgctgatcc gcgtcgcgcg
     3301 ggatcctaat cccacgggaa gccaggagct gttgcacgaa acccgagcaa tcgaacccca
     3361 ggggtgtccg ccctccccac aggtagggaa cgcccaggaa cgacccgacc tgaagagcca
     3421 gccggggaga acggccgacg ctgcggcgaa ccgcggcctt tggaatccag ccggaccggc
     3481 catcggggag ccgaaccggg cgccaggcag atccggacct cttgcgcgtc ccaatgggtg
     3541 cgagcctcgt gttgaagaac accggcgaaa tgaggagcct cccccccgcg ctggcccgga
     3601 cctcggcgtg gtgggccacc acccgcagcc ggccgccctc cagccaggat gccgcctcgg
     3661 gcgcggtgac cggcatcagg ccccaggaac ggacccaccc ccggtaatga tcgactcgat
     3721 tctcgacgcg gacccactgc cgagtccggt cgctggcaag gcagcgcacg acctcgccga
     3781 gcagaagctg gctacgcagc tcggcccgat gatcggggag tcgacgcagg tcgagggccg
     3841 cgatgttcac gatcgccagc ttccctttcg cctccgaatc gcgagtcttc atccttactc
     3901 cccgatcggg ctcatcaacg tgtagcgtcc gaatccctcg cggtccccaa agacgaaggt
     3961 gcggacgggg ttgtggtagt accagatctc gagctggggg gtgtcggacc cggctgcccg
     4021 gctctccacc tggtccggag gtccgtaacg gatgtagatc cttcccatgt cggaccgcca
     4081 acccggcccg tagccctgaa agtgccgctc cgcgtgccgc acccggcgga tgaactcgat
     4141 gagcgcctcg ttccgggggg tatcgggcgt ggggtcgcgc cgagcccaga acgcctccca
     4201 gcgcgccacc tgaagcgtgg agtcggcgtc ccggatcccg cgaatctctt cggggcgact
     4261 gatgtaactc aacggttcga gcaacttttc cagggcggca ccccgcggcg gtccgctctc
     4321 ttccacctcg aatgtccgat ccaccttcca tcgcgaccta ccttcggtta gctcgacctc
     4381 gaaggcgtag cgcccgacga ataggtcgct ctcggtcggg cgaatgatca ccggatccgc
     4441 ggatcgcttc acggtcaccc gagtgtcgcc tcggatgatc tgcgctccca cgtcgtccac
     4501 gatccggtag ccgaaccgat attcgcgcgg ccaggagcca tcgcgccggt cgaacaatcg
     4561 ggcgcgcgcc gccagccact gcacgtcgcc cccataactc cgcgtgtcct tgacccggaa
     4621 cgagc
//
LOCUS       DASTXO010000267         2245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1674696, whole genome shotgun sequence.
ACCESSION   DASTXO010000267 DASTXO010000000
VERSION     DASTXO010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674696"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VFU96_05740"
     CDS             complement(<1..366)
                     /locus_tag="VFU96_05740"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU5112800.1"
                     /translation="MTNPTLPPGQIETKRFPIVGERTPSADLTDPGSWRMELGGLVRK
                     QLELDLEAFRSRADQELNIDIHCVTSWTRFDSTWTGLPLRVFLDEAEPLAGAAFVSFE
                     AYSERGHHTSLPFEVAREDC"
     gene            complement(363..1412)
                     /locus_tag="VFU96_05745"
     CDS             complement(363..1412)
                     /locus_tag="VFU96_05745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU5112801.1"
                     /translation="MKKRLIIGGGIALGLLLIAGAVFAMSTWGDVNRVSIDRDDPVVE
                     EPAEQTPDEEPESNPGQAVGIPEGMEVFLLVGSDSRDELEDASGFGDFAGQRADVVMV
                     FLRPADGSRAALLSLPRDLLVENVCSDGGEHKLNDALQGCAAVNGPTALTLTVESVIG
                     QQVDHFALIDLAGFQEAVDAVGGYEICLERAVRDQRAKLELPAGCTQATGAQTLAWLR
                     SRNTQEMTAEGWRVMPGVNDLTRNERQRAFLLAMMGHLSDFSSPTAIADTAQVIAPYV
                     TIDSELSFIDAVDLAWTLRGLSRGNIDELEVPVSDATTDSGAAVLVAGVDIADLVDDY
                     LAPEIAEDPNGAFAG"
     gene            complement(1487..2029)
                     /locus_tag="VFU96_05750"
     CDS             complement(1487..2029)
                     /locus_tag="VFU96_05750"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEU5112802.1"
                     /translation="MWDRLHLIQDLALGDDLHLAAVLAPLYEDDEGDVRLVLTKRPET
                     MPTHAGHISFPGGRAHPEDSGPIETALREAHEEVGIEPDRVEVLGFLPPIDTVEFKPW
                     VVPVVGRVATPLNLVPSEREVARIHTPRLDELADGDRWWHVPWDGRQVWYYDLDGDTL
                     WGATAHMVRSLLGLPEYVNS"
     gene            2066..>2245
                     /locus_tag="VFU96_05755"
     CDS             2066..>2245
                     /locus_tag="VFU96_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112803.1"
                     /translation="MPKQLLETDRLRLQRFSRRDHGTYDEAARASLPELNQWLPWARL
                     DYEHGDTTAFIRESMQ"
BASE COUNT          414 a          734 c          722 g          375 t
ORIGIN      
        1 acagtcttcc ctcgccacct cgaaaggcag cgaggtgtga tggccccgct ccgagtaggc
       61 ctcgaacgag acgaaggctg ctcctgccaa cggctcggcc tcgtcgagga acaccctcag
      121 cggcagaccg gtccaggtcg aatcgaagcg ggtccagctg gtgacgcaat ggatgtcaat
      181 gttcagctct tgatcggccc gggagcgaaa tgcctcgagg tcgagctcca gctgcttcct
      241 gaccaacccg cctagttcca tccgccacga accggggtcg gtgagatcgg ctgacggcgt
      301 gcgctcgccg acgattggaa agcgcttggt ctcgatctgg cccgggggga gggtggggtt
      361 ggtcaaccgg cgaaggcgcc gttggggtcc tcggcgatct ccggagccag gtagtcatcg
      421 accaggtcgg cgatatcgac cccggccacc aggaccgcgg cccccgagtc ggtggtggca
      481 tcggagaccg ggacctccag ctcgtcgatg ttcccacggc tgagcccgcg cagggtccag
      541 gcgagatcca ccgcgtcgat gaacgagagc tccgagtcga tggtgacata aggagcgatc
      601 acctgggcgg tgtcggcgat ggcggtgggg ctgctgaagt cggagagatg gcccatcatc
      661 gccagcagaa acgcccgctg gcgctcgttt ctggtcaggt cgttgacgcc cggcatgact
      721 cgccagcctt cagcagtcat ctcttgggtg ttgcgcgacc tcagccaggc cagggtttgg
      781 gcaccggtgg cctgggtgca tccagccggg agctcgagct tggccctttg atcccgaacc
      841 gcccgctcga ggcagatctc atagccgccc acggcatcga cggcctcctg gaagcccgcc
      901 aggtcgatga gagcgaaatg atcgacctgc tgaccgatca ctgattcgac cgtgagggtg
      961 agggcggtcg gcccgttcac tgcggcgcaa ccttgcaacg cgtcgttcag cttgtgctcg
     1021 ccaccatcgc tgcacacgtt ctcgaccagg aggtcacgtg gaagtgagag cagtgcggcc
     1081 ctggacccat ccgccggccg gaggaacacc atgaccacgt cggctcgttg cccggcgaag
     1141 tcaccgaagc cggaggcatc ttcgagctca tccctcgagt cggagccgac gaggaggaac
     1201 acctccatgc cctcgggaat gcccaccgcc tgaccgggat tcgactcggg ctcttcatcg
     1261 ggggtctgct cggcgggctc ctccaccacc gggtcatccc ggtcgatgct cacccggttg
     1321 acgtcgcccc aggtcgacat ggcaaaaact gctcccgcga tgaggagcag cccaagggcg
     1381 atgccgccgc caattatcag tcgtttcttc acaggttggc ttacagatac ccgaagtcgc
     1441 ccattctaag acaccacaga caggacgaat ctggggaggc gggagcctag ctgttcacgt
     1501 actcgggaag tccgagaagc gatcggacca tgtgggcggt cgccccccaa agcgtgtccc
     1561 catccaggtc gtaataccag acctggcggc cgtcccaggg aacgtgccac catcggtccc
     1621 cgtcggccag ttcgtccagg cgtggggtgt gaatccgggc aacctcgcgt tcactgggaa
     1681 cgaggttcaa cggagttgca acccgaccta cgaccggaac cacccacggc ttgaactcga
     1741 ccgtgtcgat cggcgggaga aaccccagta cctcgacccg gtcgggttcg atcccgacct
     1801 cttcatgggc ttcgcgcagg gcggtttcga tagggcccga gtcctcgggg tgagccctcc
     1861 ctcccgggaa ggagatgtgg ccggcgtgcg tgggcatcgt ctccgggcgt ttggtcagaa
     1921 ccaaccgcac gtcgccttcg tcgtcctcgt agagcggggc gagaaccgcg gcgagatgga
     1981 ggtcatcccc gagggcgaga tcctggatca aatgcagccg atcccacatc gagcctcagg
     2041 ttaagcccgc cgataacctg acccggtgcc gaagcaattg ctcgagacag atcgcctcag
     2101 gctgcagaga ttcagccggc gcgaccacgg cacctacgac gaggcggccc gagcgtcgct
     2161 tcccgaactg aaccaatggc tcccctgggc acggctcgac tacgagcacg gggacaccac
     2221 ggctttcatc cgcgaatcga tgcag
//
LOCUS       DASTXO010000268         2233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_201002, whole genome shotgun sequence.
ACCESSION   DASTXO010000268 DASTXO010000000
VERSION     DASTXO010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201002"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VFU96_05760"
     CDS             <1..588
                     /locus_tag="VFU96_05760"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEU5112804.1"
                     /translation="RHSTATWSQVQTGEAEVTRKTRRDLILFGVGMGAVVAAVAVAGQ
                     GGNEVDNEVWRWLWTIFAVVMGIGEIFTAGFFLLPFAIGAGAAAILAWAGAAVVAQWL
                     VFFGVSLFALAYLRRFIHRQDEGEQPRIGANRWVGMQGVVIDDIDPISGNGMVRVELQ
                     EWRAIAPQPVPAGQTIVVTDVQGARLVVEPLETQS"
     gene            623..1567
                     /locus_tag="VFU96_05765"
     CDS             623..1567
                     /locus_tag="VFU96_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015920123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU5112805.1"
                     /translation="MQAVIVLFAVLALVLILIAAAGFRVVRPFEKGLIEFLGRYQRTV
                     DSGLRWVMPFVKRLTKVDMREQVVDVPPQEVITKDNVVVTVDAVIYYEATDPVKLQYN
                     VGNFILAATKLAQTNLRNVVGDLDLDSALTSREVINAKLRQILDDATDKWGVRVVRVE
                     LQRIEPPADVTDAMHRQMKAERDRRAQVTEAEGAKAAAVLRAEGIQQSQILEATGQAE
                     AIKRVADAEQYQLLTVAEGEGQAIERVYGAIHNGDPTPDLIAIRYLQTLEKVAEGQAT
                     KIFLPLDTAGVLGSVAVIGEMFREGASGPGRAPDSQSQ"
     gene            complement(1553..>2233)
                     /gene="aroF"
                     /locus_tag="VFU96_05770"
     CDS             complement(1553..>2233)
                     /gene="aroF"
                     /locus_tag="VFU96_05770"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU5112806.1"
                     /translation="RSVAKEGAVLLRGGAFKPRTSPYSFQGLGEDGLEMLARARAETG
                     LGVVSEVVAPEDIVLMNRYVDMLQVGSRNMQNFRLLSAVGEQPAPVLLKRGLMSTIEE
                     MLLAAEYIVGAGNPRVVLCERGIRSFETATRGTLDISAVPVLKERSHLPVIVDPSHAA
                     GKRSLVPSLAAAGVAAGADGLLIEVHDRPEEALSDGPQSLTLDGFADTMETVAKIAGA
                     LDRPLLTL"
BASE COUNT          412 a          703 c          740 g          378 t
ORIGIN      
        1 cgtcattcga ccgccacctg gagccaggtg cagacaggtg aggcggaggt gacacgcaag
       61 accaggagag acctgatctt gtttggagtc ggcatgggcg cggtggttgc cgccgtcgcc
      121 gttgccggac aaggaggcaa cgaagtggac aatgaggtct ggcggtggtt gtggaccatc
      181 ttcgccgtcg tcatggggat cggtgagata ttcaccgccg ggttctttct cctccccttc
      241 gccatcggcg ccggcgctgc cgccatcctg gcctgggccg gggcggctgt cgtcgctcaa
      301 tggcttgtgt tcttcggggt ctcactcttt gccctcgcct atctgcgtcg tttcatccat
      361 cgtcaggacg agggggagca accgcggatc ggggcaaacc ggtgggtcgg gatgcaagga
      421 gtcgtcatcg acgacattga cccgatcagc ggcaacggga tggtcagggt ggagcttcag
      481 gaatggaggg cgatcgcccc acagccggtg ccggccggtc agacgatcgt cgtcaccgac
      541 gtccaagggg ctcgtctcgt cgtcgagcct ttggagaccc agtcgtgagg aagtcgggta
      601 agagtcatct gaaaggagag acatgcaagc tgtgatcgtc ctgttcgccg tgctggcgct
      661 ggtgctgatc ctcatcgctg ccgccgggtt cagagtggtc cggcccttcg agaaagggct
      721 gatcgagttc ctcggccgtt accaacggac ggtggactcg ggtctgcgct gggtgatgcc
      781 cttcgtgaag aggctgacaa aagtcgacat gcgggagcag gtggtcgatg tccctcctca
      841 ggaggtgatc accaaagaca acgtcgtggt caccgtcgat gccgtcatct actacgaggc
      901 gaccgacccg gtgaagctgc agtacaacgt gggcaacttc atcctggctg cgaccaagct
      961 cgcccagacc aacctgagga acgtggtcgg cgacctcgat ctcgattcgg cgctgacctc
     1021 tcgtgaggtg atcaacgcca agctccgcca gatcctcgac gacgccaccg acaaatgggg
     1081 cgtccgcgtg gtccgggtcg agctgcagag gatcgagcca ccggccgacg tcaccgatgc
     1141 catgcaccgc cagatgaagg ctgagcgcga ccgtcgcgcc caggtcaccg aagccgaagg
     1201 ggccaaggca gccgcggtgc tcagggccga agggatccaa cagagccaga tactcgaggc
     1261 cacgggtcag gctgaggcga tcaagcgggt ggccgacgct gagcaatacc agctgctcac
     1321 tgtcgccgag ggcgaggggc aggccatcga aagggtctat ggcgccatcc acaacggtga
     1381 ccccactccg gatctgatcg ccatccgcta cctgcagact ctggagaagg tggccgaggg
     1441 ccaggcgacc aagatcttcc tacccctcga caccgccggt gtgctgggca gcgtcgcggt
     1501 gatcggcgag atgttcaggg aaggagccag cgggccgggc agggcaccgg actcacagag
     1561 tcagtaaggg ccgatcgagg gcaccggcga tcttcgcgac ggtctccatc gtgtccgcga
     1621 agccgtccag ggtcaggctt tgcgggccat cgctgagcgc ctcctcgggc cgatcgtgca
     1681 cctctatcag caagccgtcg gccccggccg cgactcctgc ggcggcgagg ctcggaacca
     1741 gggagcgctt ccctgcggca tgactgggat cgacgatcac aggcagatgg gaacgctcct
     1801 tgagcacggg aacggcactg atgtcgaggg tgccccgggt cgcggtctcg aaggaccgga
     1861 ttccccgctc gcacagcacc actcgcgggt tcccggcacc gacgatgtac tcggcggcga
     1921 ggagcatctc ctcgatggtc gacatcagcc ctctcttcag caaaaccgga gcgggctgct
     1981 ctccgacggc cgagaggagg cgaaagttct gcatgttccg gctccccacc tggagcatgt
     2041 cgacatatcg gttcatcaag acaatgtcct ccggggcaac cacctcggac acgactccga
     2101 gaccggtctc ggcccgggcc cgggccagca tctccaagcc gtcctccccc agtccctgga
     2161 agctgtacgg cgaggtccga ggcttgaagg caccccctcg caacagaacc gcgccttcct
     2221 tggccactga tcg
//
LOCUS       DASTXO010000269         3961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1011437, whole genome shotgun sequence.
ACCESSION   DASTXO010000269 DASTXO010000000
VERSION     DASTXO010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011437"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3182
                     /gene="gltB"
                     /locus_tag="VFU96_05775"
     CDS             <1..3182
                     /gene="gltB"
                     /locus_tag="VFU96_05775"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEU5112807.1"
                     /translation="GETGQVLHSDEVKQALATARPYGDWVNTETLRIQASFDSLADDR
                     FDAAALSRVFGYTAEERRLVIADMAQGDNPLGSMGDDTGLAVLATKPRRLTQYFHEQF
                     AQVTNPPMDPIREKLVMSLRIQLGKRGPLLEDRPQQAHLIELSSPILSDAELESIVRS
                     GDARFFSHWIATTWAVADGPEGMEGRLEEICEEATQAVKLGATIIVLSDRETEATRAP
                     IPMLLALGAVHHRLIDQGVRGSVSLVVVSGEPRDPHDLACLVGFGASAVNPYLAIEHA
                     RDLAASGAIDVDPVAAQENFRQAMEGGLLKIMSKMGICTLSAYRGSELFEVIGLSEQV
                     CRKAFRNAPRRLRGVGAKELARQALQRHEAYTSGVPDPGGFYKHRRGSDTHVTSPMVV
                     LGVQKAVRSGNPEDWDKYIELISDRDPALVRDLLTFVQRDPVPLDEVEPVEAIMRRFT
                     TAAMSHGALSAEAHVALAEAMNLIGGHSNSGEGGEDPVRFGTPMNSAIKQVASGRFGV
                     TPAYLHSADELQIKMAQGSKPGEGGQLPGHKVSAEIARLRYTEPGVTLISPPPHHDIY
                     SIEDLAELIYDLKAFKPTVLVSVKLVSEPGVGTIAVGVAKADADVITISGSEGGTGAS
                     PLVSIKHAGSPWELGLAEAHQVLTANGLRSRVLLETDGGLRTGRDIIVAALLGAERFG
                     FGTLPLLAMGCKMVRQCHLNTCPVGIATQQEDLRAKFTGSADQVVLLFRRLAEEARRH
                     MAALGARSIADLVGRADLLRPIDPQHPLSADLSAMLLASKGGKVHEGYRRTPLSGLED
                     RLVADSTDAIAGGEPVELSYPIRNVDRAIGARLSGEITARHGGDALPASTIVVKLSGT
                     AGQSFGAWLCNGVDLHLEGTSNDYLGKGMGGGLISVSPRRPGASVPHGAGNAVLYGAT
                     GGSVFLAGRVGQRFAVRNSGATAVIEGCSDHGCEYMTGGKVLVIGDVGRNFAAGMTGG
                     VAYVWDPDLSLKAMLADTAPSARRPSDTDLADIRSLIEAHLRHTASPVAEQILDDWNR
                     QQGAFWVISASAPPRKPIGVEVTEVPATG"
     gene            3179..3490
                     /locus_tag="VFU96_05780"
     CDS             3179..3490
                     /locus_tag="VFU96_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112808.1"
                     /translation="MSAHDDLLGRIQAHLRGRGELSEGRVLDGDGYFVDGHLVVAVMG
                     DDLCINVGREKWDDTLAVDGVRPLLFADLPVPGWVMVDGASVAGDESLAGWIESALAR
                     A"
     gene            3477..>3961
                     /locus_tag="VFU96_05785"
     CDS             3477..>3961
                     /locus_tag="VFU96_05785"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU5112809.1"
                     /translation="MLEPEHIVVMGAMGSGKTTLGRRLADALGREFHDSDRAIERRIG
                     KSGREIAETEGVEALHRLEKDVFLEALADHGPAVIAAAASVIEDTEVRRGMAGAFCIW
                     VTADPRILADRAARGDHRRSVAVSEHLESRDRLFGEMADLVIDTGDRSASETAALVLE
                     E"
BASE COUNT          667 a         1280 c         1342 g          672 t
ORIGIN      
        1 atggcgagac cggccaggtg ctgcactccg acgaggtgaa gcaagcgctg gccacggcgc
       61 ggccgtacgg ggactgggtg aacaccgaga ccctccgcat ccaggcttca ttcgacagtc
      121 tcgccgacga ccgcttcgac gcggccgctc tgagccgcgt gttcgggtac accgccgagg
      181 aacgccgact cgtcatcgcc gacatggcgc agggtgacaa cccgcttggc tcgatgggcg
      241 acgacaccgg gctcgctgtc ctcgccacca agccacggcg cctcacccag tacttccacg
      301 agcaatttgc acaggtgacc aacccaccga tggacccgat aagggagaag ctggtcatgt
      361 cacttcgcat tcagctcgga aagaggggcc cactcctgga ggaccggccg cagcaggcac
      421 acctcatcga gctgtccagc cccatcctct ccgatgccga gctggagtcg attgtccgct
      481 ccggggacgc gaggttcttc tcccattgga tcgcgactac ctgggcggtg gcggacggac
      541 ctgagggcat ggaaggccgt ctcgaagaga tctgtgagga agcgactcag gcggtgaagc
      601 tgggagcgac gatcattgtg ctctctgatc gggagacaga ggccacgagg gcaccgatac
      661 cgatgcttct cgccctgggg gcggtccacc atcgtctcat cgaccagggg gtccgggggt
      721 cggtctcctt ggtcgtggtg tcgggggagc cacgcgaccc gcacgacctg gcctgcttgg
      781 tggggttcgg ggcctcggcc gtgaacccct atctggcgat cgagcatgct cgcgacctcg
      841 ctgcatcggg cgccatcgac gtagacccgg tagcggcgca ggagaatttc cggcaggcca
      901 tggaaggtgg cctgctcaag atcatgtcca agatggggat ctgcaccctg tccgcctacc
      961 gaggctctga gctcttcgag gtcatcgggc tctccgagca ggtgtgccga aaggcgttcc
     1021 ggaacgcccc acgtcggctc cggggcgtcg gggcgaaaga acttgcccgc caggcgctgc
     1081 agcgtcatga ggcctacacg agcggggtgc ctgatcccgg cgggttctac aagcaccgac
     1141 gtggctccga cacccacgtc accagcccga tggtcgtgct cggggtgcag aaagcggttc
     1201 gatcgggcaa ccccgaagac tgggacaaat acatcgagtt gatctcggat cgcgatccgg
     1261 cattggttcg agatctcctc acgttcgtgc aacgcgaccc ggtgcccctc gacgaggtgg
     1321 agccggtcga agcgatcatg cgccgattca ccacggcggc catgtctcac ggcgcgctct
     1381 cggctgaggc ccacgttgcc ctggccgagg cgatgaacct gatcggcggg cactccaact
     1441 ccggtgaggg aggggaggac cctgtccggt tcggcacccc gatgaactcg gcgatcaaac
     1501 aggtcgcatc gggccgtttc ggtgtgaccc ccgcctatct ccactccgca gacgagctcc
     1561 agatcaagat ggcccaggga tcgaagcccg gcgaaggggg tcagctgccg gggcacaagg
     1621 tgtcggccga gatcgcccgc cttcgataca ccgagccggg ggtcactctg atctcgccgc
     1681 ccccgcacca cgacatctac tcgatcgaag acctcgccga gctcatctac gacctcaagg
     1741 cgttcaagcc gacggtcctc gtctcggtga agttggtcag cgagcccggt gtcggcacga
     1801 tcgcggtcgg tgtggccaag gccgacgcgg atgtgatcac catctccgga tcggagggag
     1861 gcaccggcgc ttcaccactc gtctcgataa agcacgccgg ctcaccatgg gagctcggcc
     1921 tggccgaagc gcatcaggtc ctcaccgcca acggtctccg ctcacgggtg ctgctggaga
     1981 cagacggtgg gttgcgcacc gggcgcgaca tcatcgttgc tgcgctgctc ggggcagagc
     2041 gattcggatt cgggacgctt ccgctgctcg ccatgggctg caagatggtc cgtcagtgcc
     2101 atctcaacac ctgcccggtc gggattgcca cccagcagga agatctgaga gcgaagttca
     2161 ccggatcggc cgatcaggtc gtcctcctgt tccgccgtct ggccgaggag gcacggaggc
     2221 acatggccgc cctcggagcc cgctcgatcg ccgacctggt cggacgcgcc gacctcctcc
     2281 gcccaatcga tccccagcac ccgctctctg ccgatctctc ggccatgctg ctcgcttcga
     2341 aaggcggaaa ggtccatgag ggttaccgaa ggacgccgct ctccgggctg gaggatcggc
     2401 tcgttgccga tagcaccgac gccatcgcag ggggcgagcc cgtcgagctg tcctacccga
     2461 tccgcaacgt cgatcgggcg atcggcgccc ggctatcggg tgagatcacg gcccggcacg
     2521 ggggcgatgc ccttcccgcc agcacgatcg tcgtcaagct ctccgggacc gccggtcaga
     2581 gctttggggc gtggctgtgc aacggggtgg atctgcatct cgagggaacg tccaacgact
     2641 acttgggcaa ggggatgggt ggcggtctca tctccgtctc gccgcgtcgt cccggggcat
     2701 cggtgcccca tggcgccggc aacgctgtgc tctacggggc gaccgggggc tctgtcttcc
     2761 tggcgggacg ggtcggtcag agattcgcag ttcgcaactc cggcgccacc gccgtgatcg
     2821 aaggctgttc ggaccatggt tgtgagtaca tgaccggggg caaggtgctc gtcatcggcg
     2881 atgtcgggcg caacttcgcc gcgggtatga cgggcggggt ggcctacgtg tgggacccgg
     2941 atctgagcct caaagcgatg ctggccgaca ccgcaccctc cgcccgccgt ccgtccgaca
     3001 ccgacctggc cgatatcaga tcgctgatcg aggcccacct tcgtcacacc gcaagccctg
     3061 tggccgagca gatcctcgat gattggaacc ggcagcaggg agccttttgg gtgatctctg
     3121 catccgcgcc cccgcgcaag ccaatcgggg tggaagtgac cgaggtccct gccaccggtt
     3181 gagcgcgcac gatgacctcc ttggccggat ccaggcacac ctgcgggggc ggggggagct
     3241 gagcgagggc cgggtgctcg atggcgacgg ctacttcgtc gatgggcatc tggtggtcgc
     3301 agtgatgggc gacgacctct gcatcaatgt cgggcgagag aagtgggacg acaccctggc
     3361 cgtcgatggc gtacggccgc ttctcttcgc cgacctcccc gtgccgggct gggtgatggt
     3421 cgatggagcc tccgttgccg gagacgaatc cctcgccggc tggatcgaga gcgcacttgc
     3481 tcgagcctga gcacatcgtc gtcatggggg cgatgggctc cggcaagacc acacttggcc
     3541 ggaggttggc cgacgccctg ggccgcgaat tccacgattc cgatcgtgcg atcgaacgtc
     3601 gcatcgggaa atcaggtcgt gagatcgcgg agaccgaggg cgttgaggca ttgcaccgac
     3661 tcgagaagga tgtgttcctc gaagccctcg ccgatcatgg gccggcggtg atcgccgcgg
     3721 cggcttctgt gatcgaggac accgaggtgc gccggggtat ggccggtgcg ttctgcatct
     3781 gggtgaccgc cgatccccgg atcctcgccg acagggcggc aaggggggat catcggcgat
     3841 cggtcgccgt atccgagcac ctcgaaagcc gtgaccggct ttttggggag atggcggacc
     3901 tggtgatcga cacgggtgac cggtcggcga gtgagacggc cgcgctggtt ctcgaggaat
     3961 t
//
LOCUS       DASTXO010000270         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_717518, whole genome shotgun sequence.
ACCESSION   DASTXO010000270 DASTXO010000000
VERSION     DASTXO010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717518"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VFU96_05790"
     CDS             <1..450
                     /locus_tag="VFU96_05790"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU5112810.1"
                     /translation="RGSSGGALLDSAARLVGITTAIAVSDVGAEGLGFAIPVDTALAV
                     ANDLIEDGVVSHAQLGIQGETALAEADGAEYPVGVRVTSVTSDSAYDHGGGQTNDVIT
                     ALDDVSVTSMETLLTELRTRRAGEVVEMAVTRADAGTELTVTLDGRP"
     gene            447..1517
                     /locus_tag="VFU96_05795"
     CDS             447..1517
                     /locus_tag="VFU96_05795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEU5112811.1"
                     /translation="MSDDLFSKLFDLFDQPGPINWKLAAEVARHLSGETEPVEPWAAE
                     EFRELARLAEYRIEQIAPFRVVPASDVLPLDARGWAERNLESYGSLVEPFASSVGSEG
                     PAAPLMAQLAPAIIGLQVGSLVGSLATWVVASFDAGLPQEHPGPISIVVPNIETLVPS
                     GSIDEKEIRLWIVTNEVAFRAVSQIPWIHDHVHQLATDFADAVKIDPTMLSSLMSGGS
                     DPEAIEQAIQDAGGLEGLMGGEEAEAPRLELEAFLGAITGCGRLLARRAISELSPNFD
                     AITDLRDEAREATEAAPTIGIGAVPPEATRLGDVFNQEVERRYGEDALETLWSDPTRM
                     PSAAELRDPTAWAARVLLDGWS"
     gene            1520..1981
                     /locus_tag="VFU96_05800"
     CDS             1520..1981
                     /locus_tag="VFU96_05800"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEU5112812.1"
                     /translation="MPLEIGAKAPDFTLVSQNRDQISLDDLKGRKSMIVFMPYPHTRT
                     CEAEASEIRDNWASFEGVDANVVMITTHAVATNRDWAEDNDFHFPILSDYWPHGEVSR
                     AYDTFDETFGYAKRTTYVLDSDGVIRDVIASDVLKEARPFASYLPALESIR"
BASE COUNT          409 a          727 c          719 g          383 t
ORIGIN      
        1 cgtggctcat cggggggcgc ccttctcgac agcgcggccc ggctggtggg catcaccacc
       61 gcgatcgcgg tatccgatgt gggcgcggag ggattggggt tcgccattcc ggtcgacacc
      121 gccctcgccg tggccaacga tctcatcgag gacggtgttg tcagtcacgc ccaacttggg
      181 atccagggtg agaccgcact cgccgaggcc gatggggccg agtacccggt aggagttcgg
      241 gtcaccagcg tgaccagcga ttcagcctat gaccacggag gcggtcagac caacgacgtg
      301 ataacggcgc tcgacgacgt atcggtgacc tccatggaaa ccctcctcac cgagcttcgc
      361 accagacgag ccggcgaggt tgtcgagatg gcagtgactc gggccgacgc cggtaccgag
      421 ctgactgtga cactggacgg ccgcccgtga gcgacgacct gttctcgaag cttttcgatc
      481 tgttcgatca gcccggtccg atcaactgga agctcgccgc cgaggtggcc cgccatttgt
      541 ccggggagac ggagccagtg gagccctggg cagccgagga gttccgggag cttgcgcggc
      601 tcgccgagta ccgaatcgag cagatcgccc ccttccgggt ggttccagcc tccgacgtcc
      661 tccccctgga cgcgcggggt tgggcggaac gcaacctcga gtcgtacggc tcccttgtgg
      721 aaccctttgc atcgtcggtg gggtccgaag gacccgccgc gcctctcatg gctcagcttg
      781 ctccggccat catcgggctc caagtcggct cgctggtcgg ttcactggcg acatgggtgg
      841 tcgcatcgtt cgacgccggc ctgccccagg agcaccccgg gcctatcagc atcgtggtgc
      901 ccaacatcga gaccctcgtc ccttccggtt cgatcgacga gaaggaaatc cggctttgga
      961 tcgtcaccaa cgaagtggcc ttccgggcgg tctcacaaat cccgtggatc cacgaccacg
     1021 ttcatcaact cgccaccgat ttcgccgatg cggtcaagat cgatccgacg atgctcagca
     1081 gcctcatgtc gggcgggtcc gaccctgagg ccatcgagca ggcaatccag gacgccggag
     1141 ggctcgaggg actcatgggc ggcgaagagg ccgaggcgcc tcgactcgag ttggaggcct
     1201 ttctcggggc gataaccgga tgcggccgtc ttctcgcacg ccgcgccatc tccgagctct
     1261 cccccaactt cgatgccatc accgacctcc gtgacgaggc acgggaggcc accgaagccg
     1321 cgcccacgat cgggatcggc gccgttcccc ccgaggcgac acgtctcggt gacgtgttca
     1381 accaggaggt ggaacgacgg tatggcgagg acgccctgga gaccttgtgg tctgatccca
     1441 cccggatgcc gagcgcggcc gagctgcgcg atccgacggc ttgggcagcc cgcgtcctcc
     1501 tggatgggtg gagctgacga tgccgctcga gatcggagcg aaggcaccgg atttcaccct
     1561 ggtcagccag aaccgcgacc agatctcgct ggacgacctg aagggcagga agtcgatgat
     1621 cgttttcatg ccctacccgc acactcggac gtgtgaggct gaggcatcag agatcaggga
     1681 caactgggca tccttcgagg gagtcgatgc caacgtcgtc atgatcacga cccacgccgt
     1741 ggccaccaac cgggattggg cggaagacaa cgacttccac ttcccgatcc tctccgacta
     1801 ttggccacat ggtgaggtgt cccgggcgta cgacacgttc gacgagacat tcggctacgc
     1861 caagcggacc acatacgtcc tcgattccga cggcgtgatc agagatgtga tcgcgtcaga
     1921 tgtcctgaaa gaggcacggc ctttcgccag ctatctgcct gcgctggagt cgatcaggta
     1981 actcagatca cccactctgg gtgaggccgg caccgccaga gggctatcca agtgccgatg
     2041 tggcacatgg accgacatcg acacgatcgc agtcgggttt gcgaggatac gagcatgacc
     2101 gaaaccacgc cgtcacgatg atcgccacca tccctgaagg tgcctgacgc gcgatgacgg
     2161 cacggatctc agcgccgctc gaggcatggt ggtcatcggt tcgacccggc cggggtgagc
     2221 tacgacggga ggctgtcg
//
LOCUS       DASTXO010000271         5877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_53999, whole genome shotgun sequence.
ACCESSION   DASTXO010000271 DASTXO010000000
VERSION     DASTXO010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53999"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VFU96_05805"
     CDS             <1..585
                     /locus_tag="VFU96_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016867724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112813.1"
                     /translation="AVSELLTAAADALNVPEPLVQRSAAARATANGTSVDDVLNAWAG
                     GSPAAGSAPAPAPAPSEEVAPATDDATTEVAPSASPEPEPAPEPEAAPAPAAAVVVEP
                     EPEVVLEAVGGLMTESHVSLRDDYAVSTAALDSAVESLVSTPGVYGARLTGAGFGGCV
                     IALAEPGVVKDGWVVRAVDGAEVVSDRSNYYPAP"
     gene            582..1778
                     /locus_tag="VFU96_05810"
     CDS             582..1778
                     /locus_tag="VFU96_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112814.1"
                     /translation="MSELLTAAADALNVPEPLVQRSAAARATANGTSVDDVLNAWAGG
                     SPAAGSAPAPAPAPSEEVAPATDDSTTEAAPKAGPEPEPAPEPEAALAPAAAVVVEPE
                     PEVVLEAVPFRRRVRTAMRVGAWTGAGLGLIGFVVASNWWAPTATVIGEETFTPVIQV
                     DSNAVVIGAALISIVFGAVIASLSRAAASWANPGMQLSNSPSSTAWVGALIGILLGVV
                     AGAVLTGLGTSVEGTEGVVQLPVLATVGVMAIGGAILGALTAAVTQAIGVPVAVPEGS
                     GEEIDNVRRRLRSAVGIPLTALILLVILVIPFAWALLESEELGAGGAALIGILTAGGI
                     LGFASLAGTKPNIKLSFGEVMVAVIGIGTVIVFILAVMFARSPDEEPEEEPAAEPEAR
                     ITLVVG"
     gene            complement(1807..2787)
                     /locus_tag="VFU96_05815"
     CDS             complement(1807..2787)
                     /locus_tag="VFU96_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEU5112815.1"
                     /translation="MPYRVLATDRVSKKGLAPLREDERFDVVLIKDSSDPEFAFGLKT
                     AAGLIVRSATRVDSSLLDEAPGLKVVGRAGVGVDNIDLAAASERGVAVMNAPSGNTIS
                     AAELTMALMLAVARRVTLADRSIRQGRWERSSLQGVELRGKTLGVIGAGRIGWEVAQR
                     CRAFGMDVIVYDPYLAAERVDELHPKLVPLDRLIESADVITLHVPLNAETRGLIGEDT
                     LTRMKKGAFVLNVSRGGVVDEAALARALNEGRIAGAGLDVYEHEPLSPDSPLLDAPNL
                     VLTPHLGASTKEAQIQVALEVARKVRTALAQGDLSSAVNASDVANAAGLS"
     gene            complement(2790..3887)
                     /locus_tag="VFU96_05820"
     CDS             complement(2790..3887)
                     /locus_tag="VFU96_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEU5112816.1"
                     /translation="MPIDLPPGHFFLPGPTEVHPEVLAAQTRPMIGHRGRAIEELLAG
                     LQEGLKRVFLTEQPVIVSTSSATGLMEASVRNGVAGGRVLSLVNGAFSSRFAEIAMAC
                     GHDTEVWSIEWGQVHDVAELDVRLAQGRFDAVTMTHSETSTGALQDLAAIAEVTGRHD
                     GVMLLIDSVTGVAGAEVRTDAWGLDFVLTGSQKALALPPGLAFAVASPAMLDRSAEAT
                     NKGYYFDLLPLIKSLQAFQTPSTPAISLLYALEVQLGRIMDEGIEQRWERHAAMRAAT
                     IEWVDSSRAEGVEVAVLAPEGHRSPTVTCISLPEGISGPEVVAAMRERGWVIGGGYSK
                     LKESTIRIGHMGDHDPQVLRELLGTLGEVLH"
     gene            complement(3916..4236)
                     /locus_tag="VFU96_05825"
     CDS             complement(3916..4236)
                     /locus_tag="VFU96_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112817.1"
                     /translation="MGASRVHASGILKSIAVVVVALGFVACSQTEGTARGVVTSVDGT
                     LEDVTSFVVLVEGSELEFTPTADGEYDFPLSHLREHQRTGEPVLVGWEIVGSVRYALS
                     LADG"
     gene            complement(4200..5006)
                     /locus_tag="VFU96_05830"
     CDS             complement(4200..5006)
                     /locus_tag="VFU96_05830"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEU5112818.1"
                     /translation="MLPAFHPHYDVWALAFALAFGYWYADRRLRPVLAPDAPRATAGQ
                     KWQWYGAVLALWLVSGWPIHDIGETSLFTVHMVEHMVIALVIPALMLRGTPRWLADVT
                     IGHPKVARFIRPLARPVPAFVLFNTTFIVIHWPDLVALMVQGGPPHLLVHAWLFGVSI
                     LMWLPVLSPTPAIPRLSPPVQMFYLFAQSLLPTIPASFLTFSTVPLYPVYGDAALAFG
                     LTAVADQTIAGVIMKLGGGLLLWSTIAVIWFRWTKQEREWERLESTLRAS"
     gene            complement(5011..5322)
                     /locus_tag="VFU96_05835"
     CDS             complement(5011..5322)
                     /locus_tag="VFU96_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEU5112819.1"
                     /translation="MSTSEHIHHPTPAQYWKIAVVLAVLTAIEVAIYYIHRDFDLGNL
                     NTPLLIILAALKFVIVVGWYMHLRFEKPTLRRFFTAGIILAAALYTIILTALGAIILQ
                     D"
     gene            complement(5319..>5877)
                     /locus_tag="VFU96_05840"
     CDS             complement(5319..>5877)
                     /locus_tag="VFU96_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HEU5112820.1"
                     /translation="RKLGMWLFLSSEFLFFGALISNYLLYSKRPGFPLPYPADIYDIP
                     FTSVSSFVLLMSSLTMVLAHNAQSRQDANGTRTWLFATAALGSVFLGGQVFEFTTFIV
                     EDGMKMSTNPAASAFYLLTGFHGTHVAIGVIMLLSLWGISRRRGELSDRHGLNLELVG
                     LYWHFVDIIWIVIFTVVYLLSVPPA"
BASE COUNT         1079 a         1909 c         1936 g          953 t
ORIGIN      
        1 gccgtgagtg aactgctgac tgcagccgcc gatgcgctga atgtccccga gccactggtg
       61 caacggtcgg cggcggcgag ggccacggcc aacgggacgt ccgtagacga tgtgctcaac
      121 gcatgggcgg gaggatcgcc cgccgccgga tcggcgccgg cccccgcgcc tgcaccgtct
      181 gaggaggtgg cgcccgcaac cgatgatgcg acgacggagg tcgctccctc cgcgtccccc
      241 gagcccgagc cggcacctga gcccgaagcg gcgccagcac ccgccgccgc ggtggtggtc
      301 gaacctgagc cggaggtcgt cctcgaggcc gtcggcgggc tgatgaccga aagccatgtc
      361 agcctgcgtg acgactacgc cgtctcgacc gccgccctcg attctgcagt cgagagtctc
      421 gtgtcgactc caggcgtgta tggagcccgt ctcaccggcg ccgggttcgg tggctgtgtc
      481 atcgccctag ccgaaccggg ggtggtgaag gacggctggg tggtgcgcgc cgtcgacggc
      541 gcggaggtgg tctcagaccg atccaattac tatccggcgc cgtgagtgaa ctgctgactg
      601 cagccgccga tgcgctgaat gtccccgagc cactggtgca acggtcggcg gcggcaaggg
      661 ccacggccaa cgggacgtcc gtggacgatg tgctcaacgc gtgggcagga ggatctcccg
      721 ccgccggatc ggcgccggcc cccgcgcctg caccgtctga ggaggtggcg cccgcaaccg
      781 atgattcgac gacggaggcc gctcctaaag cgggccccga gcccgagccg gcacctgagc
      841 ccgaagcggc actagcacct gccgccgcgg tggtggtcga acctgagccg gaggtcgtcc
      901 tcgaggccgt gccgttccgt cgtcgggtgc gcaccgcgat gagggtcgga gcctggaccg
      961 gcgccggcct cgggctcatc ggcttcgtcg tggccagcaa ctggtgggcg cccacggcca
     1021 ccgtcatcgg tgaagagacg ttcacgcctg tcatccaggt cgattccaac gctgtggtga
     1081 ttggtgccgc tctcatcagc attgtcttcg gagcggtcat cgcctcgctg tcccgagcag
     1141 ccgcgtcatg ggccaatccc ggcatgcagc tcagcaactc ccccagctcc accgcctggg
     1201 tcggtgccct catcggcatc ctgctcggtg tggttgccgg agccgtcctg accggtttgg
     1261 gcacctcggt ggaagggacg gaaggtgtgg ttcagctgcc tgtcctcgcc accgtcggcg
     1321 tgatggcgat cggaggggcc atcctcggcg cgctcaccgc cgcagtcact caggcgatcg
     1381 gcgtcccggt tgcggtccca gaaggctcgg gcgaggagat cgacaacgtg aggcgaaggc
     1441 tccggtcggc tgtcggcatc ccgctgaccg cgctgatctt gttggtcatc ctcgtcatcc
     1501 cgttcgcctg ggctctactc gagtcagagg agctcggcgc gggtggcgca gcgttgatcg
     1561 gcattctcac cgctggcggc atcctcggat tcgcatccct cgccggcacc aagccgaaca
     1621 tcaagctgtc gttcggcgag gtgatggtgg cagtgatcgg gatcggcacc gtcatcgtgt
     1681 tcatcctggc ggtcatgttc gcccgcagcc cggacgagga gccggaggaa gagccggcgg
     1741 cagagcccga agctcgcatc accctcgtgg tcggctgaat ccggggggag cgccgccgaa
     1801 cggcgttcag ctgagaccgg cggcgttggc cacatccgag gcgttgacgg cggaggaaag
     1861 gtcgccttgg gccagggcgg tgcgcacttt ccgagcaact tcgagggcga cttggatctg
     1921 cgcctccttg gtagaggctc cgagatgcgg ggtgagcacc agattcgggg cgtcgaggag
     1981 tgggctgtcc ggggacagcg gttcgtgctc atacacatct agacccgctc ccgcgatccg
     2041 accctcgttg agggctcgcg ccagcgcagc ctcgtcgacg acaccaccac gagagacatt
     2101 cagcacgaag gcgcccttct tcatccgcgt caaggtgtct tcgccgatca gacccctggt
     2161 ctcggcattc agcgggacgt ggagagtgat cacatctgcg ctctcgatga gacggtccaa
     2221 aggcaccagc ttcgggtgca gctcgtcgac tctctcggcg gccaggtagg ggtcgtacac
     2281 gatcacgtcc atgccgaagg ctcgacaacg ttgtgcgacc tcccatccga tccgacccgc
     2341 accgatgaca cccagggtct tccctcgcag ctcgacgccc tgcaagctcg agcgctccca
     2401 tcggccctga cgaatcgacc gatccgccaa tgtcaccctg cgagccaccg ccagcatcag
     2461 cgccatggtc agctctgcgg ccgagatcgt gttcccactc ggggcgttca tcaccgccac
     2521 cccacgctcg gacgctgctg ccaggtcgat gttgtccact cccacgccgg cgcggcccac
     2581 caccttgagc cctggggcct catccagcag ggacgaatcg accctcgtgg cgctgcgcac
     2641 gatcagacca gcggccgtct tcaagccgaa cgcgaactcg gggtccgagg agtctttgat
     2701 caagacgaca tcgaaacgct cgtcttcacg aagaggggcc aggcccttct tggagactcg
     2761 atccgttgcg agcacgcggt atggcatggt caatggagga cctcaccgag cgtgccgagc
     2821 agttcgcgga gcacctgtgg gtcgtggtca cccatgtggc cgatgcggat ggtcgactcc
     2881 ttcagcttcg aatagccacc cccgatgacc catccccgct ctctcatcgc cgccaccact
     2941 tcgggcccgg agatgccctc gggaaggctg atgcaggtga cggttgggga gcgatgtccc
     3001 tcaggagcga gtaccgctac ttccactccc tcggcccggc tgctgtccac ccattcgatc
     3061 gtggccgcgc gcatcgccgc gtgacgttcc caacgctgct cgattccctc atccatgatc
     3121 cgccccagct ggacctcgag ggcatagaga agcgaaatgg ccggcgtcga cggggtttga
     3181 aacgcctgga gcgacttgat caggggcagc aagtcgaagt aatagccctt gttcgtggcc
     3241 tcggccgacc gatccagcat cgccggcgag gcgacggcga aggccaggcc cgggggtaag
     3301 gccaatgcct tctgcgaccc ggttaacacg aaatcaaggc cccaggcatc ggtgcgaact
     3361 tcggctccgg ccacgcccgt gaccgaatcg atcaagagca tcacaccgtc atgacgcccg
     3421 gtgacctcgg cgatcgcggc gaggtcctga agtgctcctg tcgaagtctc ggagtgggtc
     3481 atcgtcaccg cgtcgaaacg gccctgtgcc agcctgacgt cgagctcggc aacatcgtga
     3541 acctggcccc actcgatcga ccacacctcg gtgtcatggc cacacgccat ggcgatctcg
     3601 gcgaacctcg aggagaacgc gccattcacc aggctcagga ccctgccacc ggccacgcca
     3661 ttgcggactg acgcctccat cagacccgtg gccgacgagg tagagacgat caccggctgt
     3721 tcggtgagga aaacccgctt cagcccttcc tgcaggcccg cgagcagctc ttcgatggcc
     3781 cgtccccgat gaccgatcat cgggcgagtc tgagcagcca acacctcggg atggacttct
     3841 gtgggaccag gtaggaagaa atgacctggc ggcaggtcga tcggcatgtg ctcctcgtac
     3901 tcggttgggc gccctctacc cgtcagccag gctcagtgcg taccgcacgc tacccacgat
     3961 ctcccaacca acgagaaccg gctctcccgt ccgttggtgc tctctgaggt gggacaatgg
     4021 gaagtcgtac tcgccgtcgg cggtgggggt gaattcgagc tcggacccct cgaccaggac
     4081 cacgaacgaa gtgacgtcct ccagagtgcc gtccaccgag gtgaccacac cgcgcgcggt
     4141 gccttcggtc tgactgcagg cgacgaatcc gagagccacc acgacgaccg cgatcgactt
     4201 caggatgccc gaagcgtgga ctcgagacgc tcccactctc gctcctgctt cgtccagcga
     4261 aaccagatca ctgcgatcgt cgaccacagg agcagccccc cgcccagctt catgatgacc
     4321 ccggcgatcg tctggtcggc caccgcagtg agcccgaacg ccagggcggc atcgccgtat
     4381 accgggtaga gcgggacggt ggagaaggtg aggaacgatg ccgggatcgt ggggagcagc
     4441 gactgggcga acagatagaa catctgaacg ggcggggaga gcctgggaat ggcgggcgtg
     4501 ggcgagagca cggggagcca catcaggatg gacaccccga acagccaggc gtggaccagg
     4561 agatgcggtg gccccccctg caccatcaga gccacgagat cgggccagtg gatgacgatg
     4621 aaggtggtgt tgaacagcac gaaggccggc acggggcgcg ccagcggccg aatgaaccgg
     4681 gcgaccttgg gatgcccgat ggtcacatct gcgagccatc tcggggtgcc cctgagcatc
     4741 agggccggta tcaccagtgc gatgaccatg tgctcgacca tgtgcaccgt gaacagagac
     4801 gtctcaccta tgtcgtggat cggccagccc gagaccagcc acaaggcgag gactgccccg
     4861 taccactgcc atttctggcc agctgtcgcc cgtggcgcat cgggtgccag caccgggcga
     4921 agcctgcggt cggcgtacca atacccgaag gcgagcgcga aggcgagagc ccagacgtcg
     4981 taatgggggt ggaacgctgg gagcatctcc tcaatcctgg aggatgatgg ccccgagcgc
     5041 cgtgaggatg atcgtgtaca gcgctgcggc gaggattatc ccggccgtga agaaccgccg
     5101 gagagtcggc ttctcgaaac ggagatgcat gtaccacccg accacgatga cgaatttcag
     5161 cgcggcaagg atgatcagca ggggggtgtt caaattgccg agatcgaaat ccctgtggat
     5221 gtagtagatc gcaacttcga tcgcggtcag cacggccagg accactgcga tcttccagta
     5281 ctgggccgga gttggatgat ggatgtgctc ggaggtgctc atgccggtgg caccgacagc
     5341 agatagacga cggtgaagat cacgatccag atgatgtcga cgaagtgcca gtagagaccc
     5401 accagctcca gattgagccc gtgacgatcg ctcagctcgc cacgtcgtcg ggagatcccc
     5461 cacaaagaga ggagcatgat cactccgatg gcgacgtggg tcccgtgaaa tccagtgagc
     5521 aggtagaagg cggaggcggc ggggttggtg ctcatcttca tgccgtcttc gacgatgaag
     5581 gtcgtgaact cgaacacctg cccaccgagg aacaccgaac caagcgcagc cgtcgcgaag
     5641 agccaggtac gggtcccgtt ggcatcctgg cggctctggg cgttgtgggc cagcaccatg
     5701 gtgagcgagc tcatcagcag cacgaatgag ctcaccgagg tgaacgggat gtcgtagatg
     5761 tccgctgggt acggaagcgg gaagccgggc cgcttggagt agagcaggta gttggagatc
     5821 agcgcaccga agaagaggaa ctccgagctg aggaaaagcc acatcccgag cttgcgg
//
LOCUS       DASTXO010000272         2642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_385813, whole genome shotgun sequence.
ACCESSION   DASTXO010000272 DASTXO010000000
VERSION     DASTXO010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385813"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFU96_05845"
     CDS             complement(<1..230)
                     /locus_tag="VFU96_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004962317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU5112821.1"
                     /translation="MTQQNPPAEGVPQVEITDEMEQSFVDYAMSVIVSRALPDVRDGL
                     KPVQRRIIYSLFENNITSGSAHRKCSKVVGDV"
     gene            complement(234..2144)
                     /gene="gyrB"
                     /locus_tag="VFU96_05850"
     CDS             complement(234..2144)
                     /gene="gyrB"
                     /locus_tag="VFU96_05850"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEU5112822.1"
                     /translation="MTATRDSYEAKDITVLEGLEAVKRRPGMYIGSTGPRGLHHLIWE
                     VVDNSVDEAMAGQASRIEVTLLGDGGVEVVDDGRGIPVEQMSKTKRSALTTVLTTLHA
                     GGKFEQGAYTVSGGLHGVGVSVVNALSTRLEAEVRRGGHVYYQEFHEGQPKTRDPKKV
                     KPIKKTGTTIRFWPSPLTFQETTEFDYDTVASRLRETAFLNRGLQITLTDRRSEPERI
                     EIFHYKGGLVDFVKHLNSLRDPLHAHIIDISDVGDDAELDVAMQWTTSYTESVLSFAN
                     NINTHEGGTHEEGFRKALTKAINDFARTKGLLKEKDENLTGEDVREGLTAVLSVRVKE
                     PQFEGQTKTKLGNTEIRSYVEKTLNRKLPEWLERYSPEARRIVDKCIGAAKAREAARK
                     ARELTRRKSGLESGGLPDKLADCSSRDPGESELFIVEGKSAAGPAKSARDSNTQAILP
                     IRGKILNVEKARLAKALQNTEVQDIFTAMGTGIGDDFDIEKARYHKIVLLADADVDGA
                     HIRTLLLTLLFRHMKPLIEYGYVYIAQPPLYRVKVGSQVHYLKDDAQLDGFKKEHPKV
                     KPTRFKGLAEMNAQELWETTMNPATRTLLQVEMEDAARAEEVFVTLMGDDVASRKAFI
                     QQNADDVRFLDI"
     gene            complement(2284..2589)
                     /locus_tag="VFU96_05855"
     CDS             complement(2284..2589)
                     /locus_tag="VFU96_05855"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEU5112823.1"
                     /translation="MSEGDLTPFRTSLEEMFRKLGMSDPMAMARIAAAWDELAGTPWS
                     GRSRPLFIQGKTLVVEAGAPSMVAFLRYGSADLVAAISGALGEGVIEKIEVRAPGTR"
BASE COUNT          411 a          828 c          853 g          550 t
ORIGIN      
        1 atcacgtcac cgacgacctt cgagcacttg cggtgggccg agcccgaggt gatgttgttc
       61 tcgaacagcg agtagatgat ccggcgctgc accggcttca gcccgtcgcg gacgtcgggc
      121 agggcccggg agacgatcac cgacatcgcg tagtcgacga agctctgctc catctcgtcg
      181 gtgatctcga cctggggcac gccttcggct ggtgggttct gctgggtcat ggctcagatg
      241 tccaggaatc ggacgtcgtc ggcgttctgc tggatgaagg cctttcgcga cgccacgtcg
      301 tcgcccatca aggtgacgaa gacctcctcg gcccgcgccg cgtcctccat ctccacctgg
      361 agcagggtgc gggtggccgg gttcatcgtg gtttcccaga gctcttgggc gttcatctcg
      421 gcgagaccct tgaagcgagt cggcttcacc ttggggtgct ctttcttgaa gccgtcgagc
      481 tgggcgtcgt ctttcaggta gtggacctgg gaccccacct tcacccgata gaggggtggc
      541 tgggcgatgt agacgtaccc gtactcgatc agcggcttca tgtggcggaa gaggagggtg
      601 aggagcagcg tccgaatatg agcgccgtcg acgtcggcat cggcgagcag gacgatcttg
      661 tgatagcggg ccttctcgat gtcgaagtcg tctccgatgc cggtgcccat cgcggtgaag
      721 atgtcctgca cctcggtgtt ctgcagggcc ttggccagcc gtgccttctc cacgttgagg
      781 atcttgcccc ggatggggag gatcgcctgg gtgttggagt cccgagccga tttggccggc
      841 ccggcggccg actttccctc gacgatgaac agctccgact cgcccggatc cctcgaggag
      901 cagtcggcca gcttgtcggg gagacccccg gactccagac ccgacttccg ccgggtcagc
      961 tcccgggctt tgcgcgctgc ctccctggcc ttggcggcgc cgatgcactt gtcgacgatc
     1021 cggcgggctt ccggggagta tcgctccaac cattcgggaa gcttgcggtt cagcgtcttc
     1081 tcgacgtagg agcggatctc ggtgttaccc agcttggtct tggtctgccc ctcgaactgg
     1141 ggctccttga cccggacgga gaggaccgcg gtcaggccct cccggacgtc ctcgccggtc
     1201 aggttctcgt ctttctcctt gagcaggccc ttggtgcggg cgaagtcgtt gatcgccttg
     1261 gtgagggcct tgcgaaaccc ttcctcgtgg gtgccgccct cgtgggtgtt gatgttgttg
     1321 gcgaaggaga gaaccgattc ggtgtacgag gtggtccatt gcatggccac atcgagttcc
     1381 gcgtcgtcgc cgacgtcgga gatgtcgatg atgtgagcgt ggagcggatc acgcagcgaa
     1441 ttgaggtgct tgacgaaatc gaccagccct cccttgtagt ggaagatctc gatgcgctcc
     1501 ggttcactgc gccggtcggt gagtgtgatc tggaggcctc gattgagaaa ggctgtctcc
     1561 cgcagccgcg aggcgaccgt gtcgtagtcg aactcggtgg tctcctggaa ggtcagcggg
     1621 ctcggccaga atcggatagt ggtcccggtc ttcttgatcg gcttgacctt cttggggtca
     1681 cgggtcttcg gctgtccctc atggaactcc tggtagtaga cgtggccccc acgtctgacc
     1741 tcggcctcga gccgcgtgga gagcgcattg acgaccgaga ccccaacacc atgcagacca
     1801 ccggagactg tgtaagcccc ctgctcgaac ttgccgccgg cgtgaagtgt ggtgaggacc
     1861 gtggtcagcg ccgagcgctt ggtcttcgac atttgctcca cggggattcc gcgcccgtcg
     1921 tccacgacct ctaccccacc gtcgccgagc agggtcactt cgatcctcga tgcctggccc
     1981 gccattgcct cgtcgaccga gttgtccacg acctcccaga tgaggtgatg cagacccctg
     2041 gggccggtgg atccgatgta catcccgggc cgtctcttga ctgcctcgag accctcgagc
     2101 acggtgatgt ctttggcttc gtaactgtcg cgagtggccg tcacgaccct ccttgtggga
     2161 ccttggggtg tgtctgctgg tggacctggg tcaggagccg ggaacccggc tgcagacgag
     2221 tttcacggat gtcattttac aggaattagc tgagaaaaac gaatcaatca aggggttcgc
     2281 cgatcaccga gtgcccggtg cccgaacctc gatcttctcg atcacgccct cgccgagtgc
     2341 cccggagatg gcggcgacca agtcggcgct gccatacctg aggaaggcca ccatcgacgg
     2401 tgcgccggct tccacgacga gggtctttcc ctggatgaac agcggtcgtg atctccccga
     2461 ccagggtgtt ccggcgagct cgtcccatgc ggcggcgata cgggccatcg ccatcgggtc
     2521 ggacatcccc agcttgcgga acatctcctc cagtgaggtt cgaaagggag tgagatcgcc
     2581 ctcgctcacc ggtgcacccc gtcaccgacc cgccatacct cgccctccac cggcacgtct
     2641 tc
//
LOCUS       DASTXO010000273         2683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1269805, whole genome shotgun sequence.
ACCESSION   DASTXO010000273 DASTXO010000000
VERSION     DASTXO010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269805"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="VFU96_05860"
     CDS             <1..1025
                     /locus_tag="VFU96_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5112824.1"
                     /translation="DAAARSLAEGGSDQYPPSQGVPALRQAIADRYGPLLGRDLDPAE
                     EVTITCGCTEALAASFLGIIDPGDEVILVEPFYDAYPVFVSMTGAVPRYVTLRPPKFE
                     IDLEELRSSFSPNTKAIVLNNPHNPTGRVFTDEELTAIAALCVEHDAIAITDEVYEEM
                     VYDGRHLRLATFEGMWERTLTLSSIGKTFSLTGWKVGWAIAPPALTAGLRAAHQFLTF
                     TTPTPVQHGAVAAMTAPVDFYEDLRSGYLKKRDLLADGLERAGFDVHRPQGTYFMMAG
                     FDGFGFSDDRSFCRHMVEAARVVAIPPSVFYHRPEDGASLVRFAFCKDEEVLSEALDR
                     LSGLGR"
     gene            complement(1032..2015)
                     /locus_tag="VFU96_05865"
     CDS             complement(1032..2015)
                     /locus_tag="VFU96_05865"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEU5112825.1"
                     /translation="MRFGAFVPQGWRLDLVGIDESEHWKTMSGVARRIEETGFESLWV
                     YDHFHTTPEPTQQVTYEAWTLMASLAAVTNTVRLGQMCTCNTYRPPSYLAKVAASIDV
                     ISGGRLEMGIGAGWYEHEHDGYGYPFLEPAPRIKMLEEGVEIMKAMWTEEVVHYEGRH
                     YRLRGAICQPKPLQSPHIPLWVAGGGEQLTLRVAARHAQYTNFGIKPEWFSQKSEVLA
                     GHCRDVGRDFDEITRSANFNIVCAETEAEATEKIGDLEGRVGRYVPAERAAEFAKQFR
                     YASGTPDQVISYLKEWEALGLGYAIVYFPDPAYDLSSLELFAREVMPAFAG"
     gene            2118..>2683
                     /locus_tag="VFU96_05870"
     CDS             2118..>2683
                     /locus_tag="VFU96_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HEU5112826.1"
                     /translation="MSAPFFDPGPDRDACGVGFIAARDLAPSFRMTRLAVECLVNLDH
                     RGARAADGTGDGAGLLTQVPHRLINRELATHGLEVPPARLGVVMCFLSPTEAAASRSV
                     VTEAIEAEGMQLLFWRSVPTDPSVLGDHARFVLPLIEQAIVTGDVEGPDFERSLYLSR
                     KLIERAAVPGLSVPSASSRTVVYKGLFTA"
BASE COUNT          455 a          883 c          831 g          514 t
ORIGIN      
        1 aggacgccgc cgctcggtca ttggccgaag gcggcagcga tcaatatcca cccagtcagg
       61 gagtgcctgc gctgcggcag gccatcgccg atcgatacgg gcccctgctg ggcagggatc
      121 tcgatcctgc cgaggaggtg accatcacct gcggatgcac agaggcactc gctgcctcgt
      181 tcctcgggat catcgaccct ggcgacgagg tgatcctcgt cgagcccttc tacgacgcct
      241 atccggtgtt cgtctcgatg accggcgccg tccccaggta tgtgactctg cgacctccga
      301 agttcgagat cgacctcgag gagcttcgat cgtcgttctc cccgaacacg aaggcgatcg
      361 tgctcaacaa cccccacaac ccgaccggtc gggtgttcac cgacgaggag ctgacggcca
      421 tcgccgcatt gtgcgtcgag cacgacgcca tcgcgatcac cgacgaggtc tacgaggaga
      481 tggtctacga cggccggcat ctccggctgg cgaccttcga aggaatgtgg gagcgaaccc
      541 tgaccctttc ttcgatcgga aagacgtttt cgctcaccgg ttggaaggtt gggtgggcca
      601 tcgccccacc agccctcacc gcagggttgc gcgccgccca tcagtttctc actttcacca
      661 ccccgacccc cgtgcagcac ggggcggtgg cggcgatgac tgctccagtc gatttctacg
      721 aggacctccg ctcgggttat ctgaagaagc gggatctgtt ggccgacggg ctcgaacggg
      781 ccgggttcga cgtccaccgc ccccagggca catatttcat gatggccggc ttcgatgggt
      841 tcggattctc cgacgatcgc tcgttctgcc gccatatggt cgaggccgcc cgagtggtgg
      901 cgatcccacc cagtgtcttc tatcaccggc cagaagacgg ggcttcgctg gttcgcttcg
      961 ccttctgcaa ggacgaagag gtcctcagcg aggcactcga ccggctcagc gggctcggca
     1021 ggtgaggtgt ctcagccggc gaaggcaggc atcacctcac gagcgaacag ctcgagactc
     1081 gacaggtcgt aggcggggtc ggggaaatag acgatggcgt agcccagccc caaggcctcc
     1141 cactccttca gatagctgat gacctggtcc ggggtgccgg aggcgtacct gaactgtttg
     1201 gcgaactcgg ccgcgcgctc cgcaggcacg taccgaccga cgcggccctc caggtccccg
     1261 atcttctccg tcgcctcggc ttcggtctcg gcgcagacga tgttgaagtt ggccgacctg
     1321 gtgatctcgt cgaagtcacg ccccacatcc cggcaatgac cggccagcac ctccgacttc
     1381 tggctgaacc actccggttt gatcccgaag ttcgtgtact gggcgtgacg ggccgccaca
     1441 cgcagggtca gctgctcacc accgccggca acccagagcg ggatgtgagg tgactggagt
     1501 ggcttgggtt ggcaaatcgc tcctcggagc cggtagtgcc gaccctcgta gtggaccact
     1561 tcctcggtcc acatcgcctt catgatctcg acgccttcct cgagcatctt gattcgcggc
     1621 gccggctcga ggaatgggta gccatagccg tcgtgctcgt gctcatacca gccggcgccg
     1681 atccccatct cgagacggcc accagagatc acatcgatcg aagcagccac cttggccaga
     1741 tatgacgggg gccggtaggt gttgcaggtg cacatctgtc cgagacgaac ggtgttggtg
     1801 accgcggcca gtgatgccat caaggtccac gcctcgtacg tcacttgttg ggtgggctcg
     1861 ggagtggtgt ggaagtggtc gtagacccac agcgactcga acccggtttc ctcgatgcgt
     1921 cgggccactc cggacatggt cttccaatgc tcgctctcgt cgatcccgac caggtcgaga
     1981 cgccacccct gtggcacaaa tgctccgaat ctcatgggaa gcgaggctaa cgaggtgcct
     2041 gcggcgtcgg cgaaacctgg ttggacctgg ctcttccgtg tttacacttc agcgctcccc
     2101 gagacaccca gggagcaatg tcggcacctt tcttcgatcc tggcccggat cgggacgcct
     2161 gtggcgtcgg attcatcgcg gctcgggatc tcgcccccag cttccgaatg acccgtcttg
     2221 ccgtcgagtg cctggtcaac ctcgaccatc gcggggcgcg cgccgcagac ggcaccggtg
     2281 acggcgccgg tctcctgacc caggtgccgc atcggctgat caaccgcgag ttggccactc
     2341 acgggctcga agttccaccg gcgcgtctcg gtgtggtgat gtgcttcctc tcgcccaccg
     2401 aggcggcggc gtcgcgatcg gtggtgacgg aggccatcga ggcggaaggg atgcaactct
     2461 tgttctggcg atcggtcccc accgatccat cagtgcttgg ggatcacgca cgcttcgtgc
     2521 tccctctcat cgagcaggcc atcgtcaccg gcgatgtaga agggccggat ttcgagcgaa
     2581 gcctctatct gtcccgaaag ctgatagaga gagcagctgt tcccgggctc tcggttccat
     2641 ccgcctccag ccggaccgtc gtctacaagg gtttgttcac tgc
//
LOCUS       DASTXO010000274         4982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1527673, whole genome shotgun sequence.
ACCESSION   DASTXO010000274 DASTXO010000000
VERSION     DASTXO010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1527673"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VFU96_05875"
     CDS             <1..305
                     /locus_tag="VFU96_05875"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU5112827.1"
                     /translation="GRLYFFVFGITGVLAGLAGGIGAPFLGAFQGAQFEVLLLALLVA
                     VVGGLGSIPGAFVAALLIGIIDSVGKSLFPELAHFTLFAPLIILLAVRPTGLFGKA"
     gene            315..2132
                     /locus_tag="VFU96_05880"
     CDS             315..2132
                     /locus_tag="VFU96_05880"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEU5112828.1"
                     /translation="MGRIFRSGVSRTKVLVLLGLLLAAVVVPPFLSPFPLSVLMLIVI
                     FAILAMSLDLLMGYTGMESLGQAAFFGVAAYTVAILSAKYDVGWQLAIPIALLASIVL
                     AAIGAALAVRLTGLFFLVMTLVFSQVLWGISHRWGTMTGGYLGLHGIERPFTFLDSEL
                     TFYYVIFGVFLISSLLMYRLVRSPFGLTLRGVKDSELRMRTSGYNVWLHKFIVFVIAG
                     AFAGVSGVLYTYSTQFISPTVLGVETSFEAMLMVIIGGAGTLTGPLIGAAVVTGLRNY
                     LSVFFESWLIILGLGYIITVFFAPQGILGLLPRRWRAASGVGQEAAAADETTTAPEDR
                     ETPAELTRPPVDRAGTEVTAGDKVETLRLEGVGKTFDHVRAVHDVSLTVYSGTRRVIL
                     GPNGAGKTTLFNLVTGVYKPSAGDIYLYGEKVTATAPHTRARMGLGRTFQVTNLFPSL
                     TVMNNVRLGVLGVQRKKYAMHVPVTWLDDVNERCQKLLDMAGMWDERDTEVANLSYGH
                     QRQLELIVTLASDPQVLLLDEPAAGLSLAESRSMVQLIKALDPGLTVLIIEHDMDVAF
                     EVANEVTVMHQGEVLAEGSVEEIRGNETVREVYFGQTYA"
     gene            2125..2838
                     /locus_tag="VFU96_05885"
     CDS             2125..2838
                     /locus_tag="VFU96_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020755365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5112829.1"
                     /translation="MPSVLELRDIHTYYGDSYVLQGVSLQVGPGQIVALMGRNGVGKT
                     TTIRSIVGFSPPERGTITFKDVDITKMPSHEIAREGISLVPQGRRIFPSLTVAEHLDV
                     FQRAGGSDKQAWNLDRISDLFPALEKRMKQKASVLSGGEQQMLAIARALLTNPELLLM
                     DEPSEGLSPLLVQQVVEVIGHLRATAELPILLVEQNLSLALNLADYVYVMKKGAVAFE
                     GPAEDLANAPDIQSTYLGV"
     gene            2861..3610
                     /locus_tag="VFU96_05890"
     CDS             2861..3610
                     /locus_tag="VFU96_05890"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEU5112830.1"
                     /translation="MRLEGKTALVTGASSGIGYAIGRRFGEEGAKVGLVANSQLEKAE
                     ELADKILADGGDAFAVKSNLTDVEDIERLVEVALDKLGHIDILVNCAGVFFIRLLGEL
                     SEDEWDTTLDVNVKGPFFLTQKVVPHMVEREGGNVIFVGSIFGPRGVPGAASYGASKA
                     ALHGLTECLAIELAPKIRVNAVAPGNIDTPMNQELYDKFGGREAFRVQYPMGRIGLEE
                     DVASAVTYLVSDEAEWITGVVMPVDGGYMAK"
     gene            3666..>4982
                     /locus_tag="VFU96_05895"
     CDS             3666..>4982
                     /locus_tag="VFU96_05895"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5112831.1"
                     /translation="MRRRELIDVAMGRTPADLVIEGGTLVNVSTAELYPADVAIKGDR
                     IAAVGDVSYAKGSDTRVIDAAGQHLSPGLVDGHLHQYHSYIGVNAFVEALLSHGVTAT
                     ADAFYGPGIVGGLPAIRFLKESFDKMPIRLLFLVPVLSWLQNRELGLTPTPGISPDEM
                     LEILDWDGCYGLEEPPYLPVVEHYPEILDLFDETLARRKVITGHAAGITDRQLQAFVA
                     VGAYTDHETVAVDDAVAKTRAGMKLFMRHGSGCIDAPELIRAYTEFGIDPRGLGFCAD
                     LASPEKLLTEGTIDHAVRTAIQLGVEPVRAIQMATLNVAEIFYAQQDIGSISPGRHAD
                     VVLLDDLETYAINSVIVGGETVFKGGQFIGQLPPTEYPASFYDTVSLSSPVTAEDLTF
                     KVDTRDNEAEVRVIAVTAGSLITTEEHERLAITEGQFAADIDRDILL"
BASE COUNT          915 a         1496 c         1569 g         1002 t
ORIGIN      
        1 tcggtcgcct ctatttcttc gtcttcggga tcaccggagt cctggccggg ctcgccggcg
       61 ggatcggtgc cccgttcctc ggggccttcc agggggccca gttcgaagtg ctcctcttgg
      121 ccctcctggt ggcggtggtg ggcggattgg ggagtatccc gggcgcattc gtcgccgccc
      181 tgctcatcgg gatcatcgac tccgttggga agagtctctt ccccgagttg gcccatttca
      241 ccctctttgc cccattgatc atcctcctcg cggtccggcc gaccggattg ttcgggaagg
      301 cctaggtcgt tcgcatgggg cgcatattcc ggtcaggagt ctcgaggaca aaggtcttgg
      361 tcctcctggg tctgttgctc gccgccgtcg tggtgccgcc tttcctctcc ccattcccac
      421 tctccgtgct gatgctcatc gtcatcttcg ccatcctcgc catgagcctc gacctgctca
      481 tgggctacac ggggatggag tccttgggtc aagcggcgtt tttcggagtg gccgcttaca
      541 cggtggcgat cctcagcgcc aagtacgacg tgggatggca attggcgata ccgatcgctt
      601 tgctggcgag catcgtcctg gcggcgatcg gggccgcgct cgcagtcaga ttgacgggtc
      661 tgttcttcct ggtgatgacc ctggtcttct cccaggtgct ctggggcatc tcacaccgct
      721 ggggcacgat gacaggtggc tacctgggac tgcacgggat cgagcgacct ttcaccttcc
      781 tggattccga gctcaccttc tactacgtca tctttggcgt ctttctcata tcgagccttc
      841 tgatgtaccg cctggtgcgc tcgccctttg gtctcacgct gcgcggggtc aaggacagtg
      901 agctcaggat gagaaccagt gggtacaacg tgtggctgca caagttcatc gtgttcgtca
      961 tcgcgggtgc gttcgcgggc gtctcaggcg tgctctacac gtactcgact cagttcatct
     1021 ctcccacggt cctcggtgtc gagacgtcgt tcgaggcgat gctgatggtg atcatcggag
     1081 gggcggggac tctcactggc cctctcatcg gagctgccgt cgtcaccggg ctacgcaact
     1141 atctgagcgt gttcttcgag agttggctga tcatcctggg cttgggctac atcatcaccg
     1201 tcttctttgc tcctcaagga atcctggggc tgctccctcg acgatggcgg gcagcgtcgg
     1261 gtgtcgggca ggaagccgcc gcggcagatg agacgacgac cgcaccagag gatcgggaga
     1321 ctccagccga gctgacccga ccgccggtgg atcgcgcggg caccgaggtg accgcgggcg
     1381 acaaggttga gactctgcgt ctcgaggggg tcggcaagac cttcgatcat gttcgtgccg
     1441 tgcacgacgt ctcactcact gtgtactccg gcactcgaag ggtgatcttg ggaccgaatg
     1501 gcgccgggaa gaccacgctg ttcaacctgg ttaccggtgt ctacaagcca tccgccggcg
     1561 acatctacct gtacggcgag aaggtgaccg cgacggcccc tcacacacgg gccaggatgg
     1621 ggctcgggcg caccttccag gtcaccaacc tgttcccttc gctgacggtg atgaacaacg
     1681 ttcggcttgg ggtgctcggc gtccaacgca agaagtatgc gatgcatgtg ccggtcacct
     1741 ggctggacga cgtgaacgag cgttgccaga agttgctcga catggcgggg atgtgggatg
     1801 agcgtgacac ggaggtcgcc aacctctcgt acggccatca gcgccagctc gagctgatcg
     1861 tgaccctcgc ttctgacccg caggtgctcc tgctcgacga gcccgccgcc ggcctgtcct
     1921 tggccgagag caggtcaatg gtgcagctga tcaaggcatt ggatcccgga ttgaccgtgc
     1981 tcatcatcga gcacgacatg gacgtcgcct tcgaggtcgc caacgaggtg acagtcatgc
     2041 accaaggaga ggttctcgcc gaaggaagcg tggaggagat ccgggggaac gagacagtgc
     2101 gcgaggtcta cttcggtcag acctatgcct agtgtcctag agctccgcga catccatacc
     2161 tactacggcg acagctacgt cctgcagggt gtttccctcc aggtcggccc cggccagatc
     2221 gtggccctga tgggccgcaa cggtgtggga aagacaacga cgatccgatc gatcgtcggg
     2281 ttctctccgc cggagcgagg gaccatcacc ttcaaagacg tggacatcac caagatgccg
     2341 tctcatgaga tcgcgaggga gggaatcagc ctggtgccgc aagggcggcg gatcttcccg
     2401 tctctcaccg tcgccgagca cctcgatgtc ttccagagag cgggcggttc cgacaagcag
     2461 gcctggaatc tcgatcgcat aagtgatttg ttcccggccc tggagaagcg gatgaagcag
     2521 aaggcgtctg ttttgtccgg cggggagcag cagatgcttg cgatcgctcg agccctgctg
     2581 accaaccctg agctcctgct gatggatgag ccgagcgagg ggctctcacc cctcctcgtc
     2641 cagcaggtgg tcgaggtgat cgggcatctc cgggcgacgg cggagttacc gatcctcctg
     2701 gtggagcaga acctctcgct cgcactgaat ctggctgact acgtctacgt catgaagaag
     2761 ggtgcagtcg ccttcgaagg gcctgccgag gatctggcga atgctccgga catccaaagc
     2821 acctatctcg gtgtgtgaac atgaaggaga aacacgaaac atgaggctcg agggaaagac
     2881 ggcgctggtc accggcgcgt catcaggcat cggctacgcc atcggccgga ggttcggcga
     2941 ggagggagcc aaagttggcc ttgtcgccaa ctcgcaactg gagaaggccg aggagttggc
     3001 cgacaagatt ctggccgacg gcggcgacgc gtttgccgtc aagtcgaacc tcaccgacgt
     3061 ggaggacatc gagcgtctcg tcgaggtggc cctggacaag ctgggccaca tcgacatcct
     3121 ggtgaattgc gccggcgtct tcttcattcg tctactgggt gagctcagcg aggacgaatg
     3181 ggacacaacc ctcgatgtca acgtgaaggg cccgttcttc ttgacgcaga aggtggtgcc
     3241 ccacatggtc gaacgagaag gcggaaacgt catcttcgtg ggttcgatct tcggcccgcg
     3301 cggagtcccc ggtgcagcgt cctacggcgc ctccaaggct gcgcttcatg ggctcaccga
     3361 atgcctggcg atcgagctcg caccgaagat cagggtgaac gcggtcgctc ccggcaacat
     3421 cgacacgccg atgaatcagg agctgtacga caaattcggc ggcagggaag cgttccgggt
     3481 tcagtatccg atgggccgaa tcgggctcga ggaagatgta gccagcgcag tcacctacct
     3541 ggtttccgac gaagcggaat ggatcacggg tgtggtgatg cctgtcgacg gtgggtacat
     3601 ggccaagtag tttcggaaac cgggagaaaa aggaccgtgg cagatgtgtt ggaggcgagg
     3661 ttgagatgag aagacgagag ctcattgacg ttgcgatggg caggaccccg gcggacttgg
     3721 tgatcgaggg cgggaccttg gtcaacgtat ccaccgccga gttgtaccca gcagacgtcg
     3781 cgatcaaggg tgaccggatc gcggcagtcg gtgacgtgtc ctacgccaag ggatcagaca
     3841 cacgtgtcat cgacgcggcc ggacagcatc tgtcgccggg tctggtcgac ggccatctgc
     3901 accaatatca cagctatatc ggcgtcaacg cctttgtcga agcccttctc agccacggag
     3961 tgacggcaac tgccgacgcg ttctacgggc caggcatcgt cggagggctg cctgccatcc
     4021 gattcttgaa ggaatcattc gacaagatgc ccattcggct cttgtttctg gtcccggtcc
     4081 tttcctggct ccagaatcgc gaattgggac tcacgcctac accagggatc agcccggacg
     4141 agatgctcga gatcctcgat tgggatggtt gctacggctt ggaggaaccg ccgtatctgc
     4201 cggtcgtcga gcattacccc gagatcctgg acctgttcga tgagaccctg gctcgtcgca
     4261 aggtgatcac cggccatgcg gccggaatca ccgatcggca actacaggca ttcgtagccg
     4321 tcggcgccta caccgatcac gaaacggttg ccgtcgacga tgccgttgcc aagacgcggg
     4381 cgggcatgaa actgttcatg cgtcacgggt caggctgcat cgacgccccc gagctgattc
     4441 gcgcctacac cgagttcggg atcgaccctc gcgggctcgg attctgcgcc gatcttgctt
     4501 caccggagaa actcctcacc gaggggacga tcgaccatgc ggttcgcacc gccatccagt
     4561 tgggcgtcga accagtccgc gccatccaga tggcgacgct gaacgttgcc gagatcttct
     4621 acgcacaaca agacatcggt tcgatatcgc cggggcggca cgccgacgtg gtcctcttgg
     4681 acgatctgga gacctacgcc atcaactcag tgatcgttgg cggggagacc gtcttcaagg
     4741 gcggtcagtt catcggtcag cttcccccga ccgaatatcc ggcgtctttc tacgacacgg
     4801 tgtcgctttc gagcccggtc acagccgagg atctgacttt caaggtcgac acccgtgaca
     4861 acgaggcaga agtccgtgtc atagctgtta ctgccggaag tctgatcacc accgaggaac
     4921 acgagcggct tgcgatcacc gaagggcagt tcgctgccga tatcgaccgc gacatccttc
     4981 tc
//
LOCUS       DASTXO010000275         3608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1233180, whole genome shotgun sequence.
ACCESSION   DASTXO010000275 DASTXO010000000
VERSION     DASTXO010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233180"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="VFU96_05900"
     CDS             <1..707
                     /locus_tag="VFU96_05900"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5112832.1"
                     /translation="MATQPHVCETGDSLAEVEERLHRTRSHGLPVVEGGRLVGIITLS
                     DIARAGNGQDRVVDAMTHRPATVVQTTPVSTALERMAVLGVGRLPVVAEDDPEQLVGV
                     FRREDAVRAYHEALTANTDVELQRARLAQRTDPGAGYYEFRIPPGSMADGRSIREVAW
                     PEGSTLVSIRRDRDVQVPSGDTVLLRGDVVTAFGTITSKDRMIERLNAGADEPTAEIW
                     LEDIGDTPISGTERGP"
     gene            741..1673
                     /locus_tag="VFU96_05905"
     CDS             741..1673
                     /locus_tag="VFU96_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112833.1"
                     /translation="MEGVGLAFVALIVLVVIGVSLYRQRQRAANMDLEDKSWLRAPTP
                     EAAPTIRTAPVRDFHVHGHEARVSFDVPLPDVEDPVLNDLLVDEAVEVVREKRHTLPI
                     SDVTEIVVFAGRDDVQEIGRTSLPSPGQLPPPMDSDVLNLTHIARDPFASQFEVDHSV
                     RYDTAVRVPSDELGPLRDDLRVPLGLDRGLRARGVDPDIVSGPEFVLGLLELFGYKVA
                     AQGEPDVHMATKDGISTFIRTENYQPGDHPELSEQTVRKFVAEFATSGADRGMLISDK
                     YGPFITHDIEATDRRIRFITRERAQGFIDSMALG"
     gene            1742..1966
                     /gene="tatA"
                     /locus_tag="VFU96_05910"
     CDS             1742..1966
                     /gene="tatA"
                     /locus_tag="VFU96_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEU5112834.1"
                     /translation="MRAQELLIVLLIVLLIFGARKLPDLARSLGASAKEFRKGVSEGS
                     EDTTEVQASEVPAEDKPAPETTAPDAQKPD"
     gene            2018..2518
                     /locus_tag="VFU96_05915"
     CDS             2018..2518
                     /locus_tag="VFU96_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5112835.1"
                     /translation="MESVPLTWEEVARVYGRKIYNFAYRLTGNSDDAADLVQEVLLRV
                     RKGLASYQPGSFEGWLWRITRNAFLDEVRKRNRRPTAPMPDEVDRWDIASSPSADVEY
                     SRISIGDDVQKALLELPYEFREAVVLCDIVGLSYEEIARAVAVPVGTVRSRIHRGRKL
                     LRESLT"
     gene            2515..2928
                     /locus_tag="VFU96_05920"
     CDS             2515..2928
                     /locus_tag="VFU96_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEU5112836.1"
                     /translation="MNHAGELISAYLDGELARSEIDQLQVHLATCGKCAAELQEMQTV
                     RSAVRSLPLLELPSGLIPEADAEVVPLRRNKGFWVGAAAAAVAVVIAIAALVTPSTPS
                     VALEELNSRYGARASLDPAFGPAKVIVPDLGDILE"
     gene            2925..>3608
                     /locus_tag="VFU96_05925"
     CDS             2925..>3608
                     /locus_tag="VFU96_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112837.1"
                     /translation="MRRVIGLVAALVLLPASAVFAADLDELLDESREASYTAEQVITC
                     STPDGARNAVIELSQASGELHIGAPVAPDLEVASGFGGWTLVREGAVVSSANVQGAGE
                     EPEPRYTVDEGTETEYLGREATLYRMTGDGVVRAELVFDVQAGALLRVVTFNADGSVF
                     CERRFVTFDPGAPPPTDAEAAPSVESAEGGVESSLPETLGNFDRLDVYQDEEGFIFAY
                     YSDGFFSFAV"
BASE COUNT          640 a         1140 c         1197 g          631 t
ORIGIN      
        1 tcatggccac ccagcctcat gtttgtgaga ccggcgactc gctggccgag gtcgaggaga
       61 ggctgcaccg aacccgatcc catggcctac ccgtggtcga gggcggccgg ctggtgggga
      121 tcatcaccct ctcggatatc gcccgtgccg gcaacggtca ggatcgtgtc gtcgacgcaa
      181 tgacccaccg accggcgacc gtggtgcaga caacgccggt atcgaccgcc ctggagcgga
      241 tggcggtgct tggtgtcggc cggctcccgg tcgtagccga ggatgacccg gagcaattgg
      301 tgggggtctt cagacgggag gatgcggtgc gcgcctatca cgaggccttg acagccaaca
      361 ccgatgtgga gttgcagcgg gcacgcctcg cccagcgcac tgatccgggg gcgggttatt
      421 acgagttccg catcccgccg ggctcgatgg ccgacggccg gtcgattcgg gaggtcgcct
      481 ggcccgaggg gtcgaccctg gtgtcgatcc ggcgggatag agacgttcag gtgccaagtg
      541 gagacacggt gctgctgaga ggcgacgtcg tgacggcctt cggcacgatc acctcgaagg
      601 accggatgat cgagcggctc aacgccggcg ctgatgagcc gaccgccgag atctggctgg
      661 aggacatcgg agacaccccc atttcgggga ccgagcgggg cccgtagaat caagagggtc
      721 cgcgagacag aggaatgtcg atggagggtg ttggactggc gttcgtcgcg ctgatcgtcc
      781 tggtcgtgat cggcgtcagc ctgtatcgcc agcgacagcg ggcggccaac atggatctgg
      841 aggacaagtc ttggcttcgc gcgcccaccc cagaggccgc gccgacgatc cgcaccgcgc
      901 cggtgcgcga tttccacgtc catggccacg aggcacgggt ctccttcgac gtcccgttgc
      961 cggatgtcga agacccggtg ctcaacgatc tcctcgtgga cgaggcagtc gaagtagtga
     1021 gagagaagcg tcacaccctc ccaatctccg atgtcaccga gatcgtcgtg ttcgccggcc
     1081 gggacgatgt tcaggagata ggtcgcactt cgcttccgtc accaggccaa ttgcctccgc
     1141 ctatggacag cgatgtcctc aacctgaccc acatcgcccg ggacccgttt gcttcccagt
     1201 tcgaggtcga ccactcggtc cggtacgaca ccgcggttcg ggtgccatcc gacgagctag
     1261 gcccgttgcg cgatgacctc agagtgccgc tcggccttga tcgcgggctg cgtgcccgtg
     1321 gcgtcgatcc cgacatcgtc agcggccccg agttcgtcct cggcctgctc gagttgttcg
     1381 gctacaaggt ggccgcacag ggtgagcccg acgtgcacat ggccaccaag gacggcatca
     1441 gcacgttcat ccggaccgag aactaccaac caggcgatca ccccgagttg agcgagcaaa
     1501 cggtgcgcaa gttcgtggcg gagttcgcca catcgggcgc cgatcgaggc atgctcatca
     1561 gcgacaaata cggccccttc atcacccacg acatcgaggc caccgatcgg agaatccgct
     1621 tcattacgag ggagagagcc cagggattca tcgattcgat ggcactcgga tgattccgtc
     1681 gtgcctggat ccaggcgagg ggtaagatgc gagcaaagga gggctagatt tgttcagcgg
     1741 attgagagcc caagaacttc ttatcgtcct gctcatcgtt ctattgatct tcggagcgag
     1801 gaagctgcct gacctggcca ggtccctcgg ggcctcggcc aaggagttcc gcaagggcgt
     1861 gtcggagggc tccgaggaca ccaccgaggt tcaggcctcg gaagtgccgg ctgaggacaa
     1921 gcccgctccc gagacgaccg ccccggacgc acaaaaaccg gactaggcgc tggaacccaa
     1981 acacgccgtc gtggcgttgt tgagctaggt ctccgcgatg gagtccgtcc cactcacctg
     2041 ggaagaggtt gcgcgtgtct atggacggaa gatctacaac ttcgcttacc ggctcaccgg
     2101 taacagcgac gatgccgcgg atctcgtcca ggaagtcctt ctccgagtgc gcaagggttt
     2161 ggcctcctac cagccgggga gcttcgaggg ctggctatgg cggatcacca ggaacgcctt
     2221 cctcgacgag gttcgaaagc gcaaccgtcg tcccacggca ccaatgccgg acgaagtcga
     2281 taggtgggac atagccagca gcccgtcggc cgatgtcgag tactcccgga tatcgatcgg
     2341 ggacgacgtc cagaaggctc tgctcgaatt gccctacgag ttcagggagg cggtcgtcct
     2401 ctgcgacatc gtcggcctct cctatgagga gatcgcacga gctgtcgcgg tgccggtggg
     2461 cacggtgcgg agcaggatcc accgcggcag gaaactcctc agggagtcgc tgacgtgaat
     2521 cacgccggag agctcatcag cgcctatctg gacggagagc tggccaggtc ggagatcgac
     2581 cagctccaag tgcatctcgc gacctgcggg aaatgcgccg ccgagctgca agagatgcag
     2641 acagtccgtt ccgcggttcg gtcgcttccc ctcctcgagc tgccatcagg gctgatcccg
     2701 gaagccgatg cggaggtcgt gccgctccga cgaaacaagg gattctgggt cggcgcggcc
     2761 gcggccgccg tcgccgtcgt catcgccatc gccgctctcg tcaccccctc gacgccgtcg
     2821 gttgcgctcg aggagttgaa cagccgttac ggggctcgcg cttccctcga tcctgcattc
     2881 gggccggcca aggtgatcgt tccagatctg ggcgacattc tcgaatgagg cgagtcatcg
     2941 gcctcgtcgc ggcactggtg ctccttccag catcggcggt gttcgcggcg gatctcgacg
     3001 aattgctcga cgagagccgg gaagcctctt ataccgcgga gcaggtgatc acctgttcca
     3061 cgcctgacgg tgctcgaaac gccgtcatcg agttgagcca ggccagtggc gagctccaca
     3121 tcggcgctcc ggtggcgccc gacctcgagg tcgcttccgg gttcggcggg tggaccctgg
     3181 ttcgcgaggg agcagtggtc agtagcgcca atgtccaggg cgcgggcgag gagccggagc
     3241 cgcgctatac ggtggacgag gggaccgaga ccgaatacct gggccgcgaa gccaccctgt
     3301 atcggatgac cggcgacggg gtggtccgag ccgagctcgt cttcgacgtc caagccgggg
     3361 ccctgctccg ggtggtgacc ttcaacgccg atggaagcgt cttctgcgag cgccgcttcg
     3421 tcacctttga tccgggcgcg ccaccgccca ccgacgccga ggccgccccg agcgtcgagt
     3481 ctgccgaggg cggcgtcgag tcgagtcttc cggagacctt gggcaacttc gaccggctcg
     3541 acgtctatca ggacgaggaa ggcttcatct tcgcctacta ctcggatggg ttcttctcgt
     3601 ttgccgtg
//
LOCUS       DASTXO010000276         2799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_385886, whole genome shotgun sequence.
ACCESSION   DASTXO010000276 DASTXO010000000
VERSION     DASTXO010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385886"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /gene="trmD"
                     /locus_tag="VFU96_05930"
     CDS             <1..537
                     /gene="trmD"
                     /locus_tag="VFU96_05930"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU5112838.1"
                     /translation="DDAPFGGGPGMVMMVEPLAGALEPLAGTHRVLFTPAGAQLTQAT
                     LDRWAGLPEVTFVCGRFEGVDQRVVDNYIDEETSVGDFVLAGGEVAAALAIEGIARLI
                     PGVVGNPGSTATESFRGGLIEEPVYTRPADFRGFEVPEVLLSGNHALIEQWRREQRMR
                     RTRERRPDLLPDGETSET"
     gene            595..939
                     /gene="rplS"
                     /locus_tag="VFU96_05935"
     CDS             595..939
                     /gene="rplS"
                     /locus_tag="VFU96_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020523395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU5112839.1"
                     /translation="MNDLHLVESAHVRDDIPEVRAGDTVKVHVRVVEGGRERVQIFEG
                     LVIARNGAGARASFTVRKLSFGVGVERVFPVHAPIISRIEVVRRGDVRRSKLYYLRDR
                     VGKSARIKELRD"
     gene            1017..1571
                     /gene="lepB"
                     /locus_tag="VFU96_05940"
     CDS             1017..1571
                     /gene="lepB"
                     /locus_tag="VFU96_05940"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU5112840.1"
                     /translation="MPGLLLTALAIAVVIKTFLVQPFWIPSESMLPTIEVNDRVMVNK
                     LAYQWGEPQRGDIVVFRDPREEEVEESIPETVIRSVLEAVGIRTRGHEDLIKRVIGLP
                     GETIEVVDNQIVVDGTPLEEPYLPEVFMPDEPAVTVGDDEVFVMGDNRDASFDSRRFG
                     PIPLDDLIGEAFVTIWPISHIGGL"
     gene            1650..1946
                     /locus_tag="VFU96_05945"
     CDS             1650..1946
                     /locus_tag="VFU96_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2469 family protein"
                     /protein_id="HEU5112841.1"
                     /translation="MEEDLERYESEVELKIYKEYHDVLPMFRYVVETERRFYLANHVD
                     LQVREANGSVFFEVEMKDAWVWDMFRPARFLEHVKVLTFRDVNVEELEQQEISI"
     gene            1970..2266
                     /locus_tag="VFU96_05950"
     CDS             1970..2266
                     /locus_tag="VFU96_05950"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEU5112842.1"
                     /translation="MGERIAAMFLTRHGLEIIGRNVEVGRGEIDLLAMDRGRHVAVEV
                     RATTADRDPIDAIGPQKRRRVTRLAGAVGAHRVDFLGVRIGSDDVVVHWVPGCG"
     gene            2300..>2799
                     /locus_tag="VFU96_05955"
     CDS             2300..>2799
                     /locus_tag="VFU96_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5112843.1"
                     /translation="MNRAEPAIRVEGLVKRYRSTTALDGLDCSIPSGAITGLLGPNGA
                     GKTTLFRSLLGLTRTDAGRILVLDQEIPAGLVEVTKKIGAIIEEPGLIRALTGRVNLQ
                     VAADQLGFGHERIEELLEFVGLQADSGRKVDEYSKGMRQRLALAASLIGDPQLLLLDE
                     PLDGLDP"
BASE COUNT          502 a          859 c          934 g          504 t
ORIGIN      
        1 gatgatgcgc ccttcggtgg tggccccggg atggtcatga tggtcgagcc cttggccggc
       61 gcgttggaac cactggccgg cacccaccgc gtcctgttca cccccgctgg ggcgcagctc
      121 acccaggcaa cgctggaccg ctgggccggg ttgcccgagg tcaccttcgt ctgcgggcga
      181 ttcgagggcg tcgaccagag ggtcgtggac aactacatcg acgaggagac ctcggtgggt
      241 gatttcgtac tggcaggtgg cgaggtggcc gcggcccttg ccatcgaggg catcgcccgt
      301 ctgattcctg gcgttgtcgg caatcccggt tcgaccgcga ccgagtcgtt tcggggtggc
      361 ctcatcgagg agccggtcta caccaggccc gccgacttcc ggggattcga agtgccagag
      421 gttctcctct cgggcaacca cgcgctgatc gagcagtgga ggagggagca gcggatgcga
      481 cggacgaggg agcgccgacc ggacctgttg ccggatggcg agacttccga gacctgatac
      541 tattcgcccc ggccgggact accgagctac tcccggcgcc tctaggagtc ctccatgaac
      601 gatcttcacc ttgttgagag cgcgcacgtc cgcgacgata tccccgaggt ccgggccggc
      661 gacaccgtca aagtccacgt ccgggtggtg gagggcggca gagagcgcgt ccagatcttc
      721 gaaggcctgg tcatcgcccg gaatggggcg ggggcgcgag cctccttcac tgtccgcaag
      781 ttgagtttcg gggtcggcgt cgagcgagtt ttccccgttc atgccccgat catctcgagg
      841 atcgaggtcg tgcgccgcgg cgacgtccgt cgttccaagc tctactacct gcgcgatcgg
      901 gtcgggaagt cggcccgcat caaggagctt cgcgactgat ctcgcacgac accggcccca
      961 tcgacgagga gacggtcacc gagacgccgc cacggcggcg ttcgttcctc gccgagttgc
     1021 cgggtttgct cctcaccgca ctggccatcg cggtcgtcat aaagacgttc ttggtgcagc
     1081 cattctggat cccgtccgag tcgatgctcc ccaccatcga ggtgaacgac cgggtcatgg
     1141 tgaacaagct ggcctatcaa tggggtgaac cgcagcgagg cgacatcgtc gtcttccgtg
     1201 acccgcgcga agaggaggtc gaggagtcga tcccggaaac ggtgatccgc tccgtcctgg
     1261 aggcggtcgg tatccgcacg agaggacacg aggacctgat caagcgggtg atcggcctcc
     1321 cgggcgagac catcgaggtc gtcgacaacc agatcgtggt cgacgggacc ccgctcgaag
     1381 agccttatct gcccgaggtg ttcatgcccg atgagccggc ggtcaccgtg ggggacgacg
     1441 aggtattcgt catgggcgac aaccgcgacg cgtcgttcga cagcaggcga ttcggcccga
     1501 taccgctcga cgacctcatc ggggaggcat ttgtcaccat ctggccgatc tcacacatcg
     1561 gcggtctctg aaaacgtaac gccggattgt cagccgttaa gtaacgagtt gtgcatatga
     1621 tcgtttgtcg gcccgttgaa gaggagcgca tggaagagga cctggagcgg tacgaatccg
     1681 aggttgagct caagatctac aaggagtacc acgacgtcct cccgatgttc cggtacgtgg
     1741 tcgagactga gcgccgtttc tacctcgcca atcatgtgga tctccaggtg cgagaggcca
     1801 acggatcggt cttcttcgag gtggagatga aggacgcctg ggtatgggac atgttccgac
     1861 cggcccggtt cctggagcac gtcaaggtgc tgacattccg cgacgtgaac gtcgaagagc
     1921 tggagcagca agagatctcg atctgaccga tcgggtcaag gtgggggaga tgggcgagcg
     1981 gatcgccgcc atgtttctca ccaggcacgg gctcgaaatc atcgggcgaa atgtcgaggt
     2041 cggtcggggt gagatcgacc tcctggcaat ggaccgggga cgacatgtcg ccgtggaggt
     2101 gagggcaacc accgcagatc gcgacccgat cgatgccatc gggccgcaaa aacgacgtcg
     2161 cgtcacacga ttggccggag ctgtgggtgc ccatcgtgtc gacttcctcg gtgtcaggat
     2221 cgggtctgac gacgtggtgg ttcactgggt cccgggctgt ggttgactgc cccgccatgc
     2281 ctcctgatcg gcccgccacg tgaaccgcgc agagcctgcg ataagggtcg agggcctggt
     2341 caagcgctat cggtccacca ccgctctgga tggcctcgat tgctcgatcc catcgggagc
     2401 cataaccggt ctgctcggcc ccaatggtgc cggaaagacg acactctttc gatcactcct
     2461 cggcctcacc aggaccgacg ccgggcgcat tctggttctc gaccaggaga tccctgccgg
     2521 cctcgtcgag gtgacgaaga agatcggggc catcatcgag gagccgggcc tgatcagagc
     2581 gctcaccggc cgggtcaacc tccaggtggc ggccgaccag ctcgggttcg gtcacgagcg
     2641 aatcgaagag ttgctcgaat tcgtcggtct ccaggccgac tcgggacgca aggtcgacga
     2701 gtactcgaag ggaatgcgac agcggctcgc actggcggcg tcactgatcg gggatcccca
     2761 gcttctcctt ctcgacgagc ccctggacgg gctcgaccc
//
LOCUS       DASTXO010000277         2067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_312456, whole genome shotgun sequence.
ACCESSION   DASTXO010000277 DASTXO010000000
VERSION     DASTXO010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312456"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VFU96_05960"
     CDS             complement(<1..657)
                     /locus_tag="VFU96_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEU5112844.1"
                     /translation="MSAFEPSQEQRTILDLGLDTIRIRAGAGTGKTTTVALVIANLVE
                     NHGVDPERILGITFTNKAASELADRVKTFLGTAVEEGRQAEVHTYHGFAAQILSEFGA
                     LAGVDTRVRVITPTFSRQILSETYHHTTYDHLDITNAHGLDKIRTLGDRMGDHLVTPA
                     ALIAHVDPRDEVWAMRAEMLETLRRYDTDKRRLRVVDYSDLVTLSTSLLTTHPELAAE
                     I"
     gene            complement(654..>2067)
                     /locus_tag="VFU96_05965"
     CDS             complement(654..>2067)
                     /locus_tag="VFU96_05965"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEU5112845.1"
                     /translation="FTSFSQVLERQAERDPTVTLDAVFRLTDEEGIESIPLLPYRPEE
                     QQVTLTTLHQAKGLEFDVVYIANAVEGVFPDLRRGRRMLRPELLSPERITDPEAQHLF
                     QIQEEMRLAYTAMTRAKSKVVWTATSAGVDQGEHRPSRFLMGAANPGEGPGPPEEQEG
                     APVSLAEAEVSLRRTLLDPGAASFERLAAAQLLARPPQPWWNPIAFAGVPESGPAQPI
                     LGQTLRLSPSQADAYATCPRQYALERRLRLSDTSSPYAQLGTLVHSALEAAESEVLGS
                     GKTHADLEDAMRHLERVWADANFGTPQLDQAWLAHARDTILRLYGQWPSDGVPVELEK
                     QVQTEIGGVEWIGYIDRLERGPDGLRVVDYKTSKSPPTIPEAKESVQLGFYASAVAQE
                     AGEPVVAAEMWFPRKDAKSVTTRSFDMDLLPDVMVRMEEVTTAILDERWEPRVNSRCN
                     KCAFRLSCPAWAEGKGAYLP"
BASE COUNT          324 a          647 c          712 g          384 t
ORIGIN      
        1 gatctcggcc gccagttccg ggtgggtggt gaggagactt gtagacagag tgaccaggtc
       61 ggagtagtcg acgaccctga ggcgccgctt gtccgtgtcg tagcgtcgca gggtttccag
      121 catctcggct cgcatcgccc acacctcgtc tctggggtcg acgtgtgcga tgagggccgc
      181 gggagtgacg agatggtcgc ccatgcgatc accgagggtg cggatcttgt ccaacccatg
      241 ggcgttggtg atgtcgagat ggtcgtatgt ggtgtggtga taggtctcgg agaggatctg
      301 ccgggagaac gtgggggtga taaccctgac cctcgtgtcc accccggcga gggcaccgaa
      361 ctcggacagg atctgagcgg cgaagccgtg ataggtgtgg acctcggcct ggcgaccctc
      421 ctcgaccgcg gtcccgagga acgtcttcac tcggtcggca agctcagatg ccgccttgtt
      481 ggtgaatgtg atcccgagga tgcgctccgg gtcgacgccg tgattctcca cgaggttggc
      541 gatcacgagc gccacggtgg tggtcttccc ggtgcccgcg ccggcccgga tccggatcgt
      601 gtccagacca aggtcgagga tcgtcctctg ctcctgggac ggctcgaatg cgctcatggc
      661 agatatgcgc ccttgccctc ggcccaggcc gggcatgaca acctgaaggc gcatttgttg
      721 cagcggctgt tgaccctcgg ctcccatcgt tcgtcgagga tcgccgtggt cacctcttcc
      781 attctcacca tgacgtcggg aaggagatcc atgtcgaacg acctcgtggt gacggatttg
      841 gcatccttgc gggggaacca catctcggcc gcgaccaccg gttctccggc ctcctgggcc
      901 acagctgacg cgtagaagcc cagctggacc gactccttcg cctcggggat cgtcggcggg
      961 ctcttcgagg tcttgtagtc gacgacccgc agaccgtccg gccctctctc cagccggtcg
     1021 atgtagccga tccactcgac gccaccgatc tcggtctgca cctgcttctc caactcgacg
     1081 gggaccccgt cgctgggcca ttggccgtac agccggagaa tggtgtcgcg ggcgtgggcc
     1141 agccaggcct ggtcgagctg aggggtgccg aagttcgcgt cggcccacac ccgttccagg
     1201 tgcctcatcg cgtcttccag atcggcgtgg gtcttgcccg atccgaggac ctcgctctcc
     1261 gcggcctcca acgccgaatg gaccagtgtc cccaactggg cgtacgggga ggaggtgtcg
     1321 ctcaggcgaa gacggcgctc cagcgcgtac tgccggggac aggtggcata ggcatcggct
     1381 tggctcgggg agagacggag agtctgcccg agtatcggct gggccggccc tgactcgggc
     1441 acaccggcga aagcaatcgg gttccaccac ggctgtgggg ggcgggcgag gagttgtgcc
     1501 gcggcgaggc gctcgaacga cgccgcgccg gggtcgagaa gcgtccgtct cagtgagact
     1561 tcggcttcag ccagcgagac tggagcaccc tcctgctcct cgggtgggcc gggtccctcc
     1621 ccgggattcg cggcgcccat gagaaagcga gacggtcgat gctcaccctg atcgacaccc
     1681 gccgacgtgg ccgtccacac cactttggac ttggcccggg tcatggcggt gtaggcaaga
     1741 cgcatctcct cctggatctg aaagagatgc tgggcctcgg ggtcggtgat tcgctccggg
     1801 gacagaagct ctggcctgag catcctgcgt ccacgtcgga ggtcggggaa caccccttcg
     1861 accgcgttgg cgatgtagac gacgtcgaat tcgaggccct tggcctggtg cagggtggtc
     1921 agcgtcacct gctgctcctc gggacggtag ggcagaagcg ggatcgactc gatcccctcc
     1981 tcgtcggtca gcctgaatac ggcgtcgagt gtgacggtgg ggtcgcgctc cgcctggcgc
     2041 tccaacacct gtgagaagga tgtgaag
//
LOCUS       DASTXO010000278         3172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_681145, whole genome shotgun sequence.
ACCESSION   DASTXO010000278 DASTXO010000000
VERSION     DASTXO010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681145"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VFU96_05970"
     CDS             <1..250
                     /locus_tag="VFU96_05970"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEU5112846.1"
                     /translation="VVDLAEVPTPAAGEHYEVWVLRKASGGEMEAVGVFRPTSPTVDL
                     RFTLPGPGDYQAVDVSVEEDGGAVGLRRPSIRGGRCAP"
     gene            456..1022
                     /locus_tag="VFU96_05975"
     CDS             456..1022
                     /locus_tag="VFU96_05975"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012998200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5112847.1"
                     /translation="MPYAIRQLDASTWDAFAELVERNNGVYGGCWCIGYHPECGEKGI
                     NYREAKEDRVRTGRAHAALVIDENGAAQGWCQYGSPEELPGIKHKREYDKDAPPRPDW
                     RITCIFVDKKHRGRGIARAGLEGALDQIARLGGGLVEAIPEVTAGREAQGRFLFSATV
                     ELFEQYGFTRGRRVGKHAWIVSRAVEPV"
     gene            complement(1745..2071)
                     /locus_tag="VFU96_05980"
     CDS             complement(1745..2071)
                     /locus_tag="VFU96_05980"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU5112848.1"
                     /translation="MSEFTILRLDDGSGLKVIGELDAATAPQLTTALSGASLEGEVVL
                     DLSELTFLDSSGVRVIVELAATRNGNGPVVISNASPAVVRVFEILGLNQHRGIELRRA
                     TKQAPR"
     gene            complement(2174..2518)
                     /locus_tag="VFU96_05985"
     CDS             complement(2174..2518)
                     /locus_tag="VFU96_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112849.1"
                     /translation="MIGAACRRAWAAVFREYPSRPSPGATRASVVESQEIEKFPDLVV
                     NLRRMAHPNCGVKLVSIPSALAGLRHVRAVNEVGDDPLGGPLGDPNGVCHFAEPRRGI
                     ALETQKHLRVAC"
     gene            complement(2593..2874)
                     /locus_tag="VFU96_05990"
     CDS             complement(2593..2874)
                     /locus_tag="VFU96_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013006167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU5112850.1"
                     /translation="MGIIAFIILGLLAGGIAKAIMPGDDPGGWIVTALIGIAGALIGG
                     FLAGVLFGADPMDEFFDISSWLTAIVGSILLLIIYRLVVDRRGGRALHT"
BASE COUNT          600 a         1004 c         1029 g          539 t
ORIGIN      
        1 cgtcgtcgac ctcgccgagg tgcccacgcc cgcggcgggg gagcactacg aggtctgggt
       61 cctccgcaag gcctcgggcg gcgagatgga ggccgtcggc gtcttcaggc ccacgagccc
      121 gaccgtcgac ctccggttca cgcttccggg gcccggcgac taccaggcgg tggacgtctc
      181 ggtcgaggag gacggcggcg ccgtcgggct tcgtaggcct tcgatcagag gcgggcgatg
      241 cgcgccttga ctggtgtcac gaggtggagc caccacgccg agagctgtcg agtaagaacg
      301 ggaggcggca ccgcttccgg cgctaacaac gagcagtctt ggcacgaacc tccggcgaca
      361 agctgcgtca gcggggggct ggcaagcgat tgacccgaga cggatcgtca ccgagatcca
      421 ggacgatgaa aacacctact atcgatggac cgaccatgcc gtatgcgatc cgacaactcg
      481 acgcatcaac atgggacgct ttcgccgagc tcgtcgagcg caacaacggg gtttacggtg
      541 gctgttggtg catcggctat caccccgagt gcggagagaa gggaatcaac tatcgcgagg
      601 ccaaggagga tcgcgtccgc acggggcgcg ctcatgccgc gcttgtcatc gacgagaacg
      661 gcgccgccca gggctggtgc cagtacggta gcccggagga gctcccgggc atcaagcaca
      721 agcgcgagta cgacaaggac gctccacccc ggccggattg gcggatcacc tgcatcttcg
      781 tcgacaagaa gcatcgcggt cgaggcatcg cccgggcagg cctggaggga gcgctcgacc
      841 agatcgcccg tctcggcggc ggcctcgtcg aggccatccc cgaggtgacc gccggtcgcg
      901 aggcacaggg ccgcttcctc ttcagcgcga cggtcgagct cttcgagcag tacggcttca
      961 cccggggtcg ccgcgtcggc aagcacgcct ggatcgtgag cagggcggtc gagcccgttt
     1021 gagcatctgg gtacgtcggc ttcgggcgag gccggctccc cagcaaacac aaggagcccc
     1081 gccgccagag tcgaggttga ccacgagttg accagttcgt ggccagggcg ggctacgcag
     1141 tatccggcga gcaggctccg atgcccgagc gactcgtgca gccgtgccgt agatgagttc
     1201 accgccaggg ctgaagaagt ggagctactg gagcgacgcc gcgagctagt gccctcttgc
     1261 accgactgcg cctggcgagc gttcgcgtgc gccacttcca agagccggtc agtgcccgtc
     1321 ggtcaccgtc ttcgggtagt aaggtcgcat ggctgaccga ctccgggctc gtcctcctcg
     1381 tggacgagtg tgcgggcaga tcgcatcgaa agcccccagc aggatgatca atatccaagc
     1441 ccagataggc acttgccctc cttcctagtg tgcgctccga gtacccgttt cgagaccggt
     1501 actaacggtt tcctctcctt cagaactttc ctcaggctcg accggcgtct cggcagccca
     1561 gcccgcgagg tgggtcatcc agatcgcgtt tagcgacatg aaaccgacga accagagatc
     1621 gtggcgaagg gcgattcaga gcgcgaggcg agggtgaacc aggcgatcgc caaccagtag
     1681 tgcaggactc gccgctgctt ggcgttcgta cggtcgatca cccattcgcc cgagagcaac
     1741 ctgttcaacg gggagcctgc ttcgtcgcac ggcgcagttc gatgccgcga tgctggttga
     1801 ggccgaggat ctcgaatacc cggacgaccg cgggagacgc attagagatg accaccggcc
     1861 cattgccgtt tcgcgttgcc gctagctcga cgatcacgcg cacaccagag ctatcgagga
     1921 acgtgagctc actcagatcg agaacgactt cgccctcgag cgatgccccg gaaagcgcag
     1981 tcgtcagctg tggggccgtc gccgcgtcaa gctctccgat gaccttcagt ccgcttccgt
     2041 cgtcgagccg caagatcgtg aactccgaca tctcaggatc cctcctggat ggacgtggta
     2101 cagttttgac gaccagtaat ttaggaaatt cgtatccggt atgtcaagtc ctgaatcaaa
     2161 gcgctggcac ttcctaacaa gccacgcgca ggtgcttctg tgtctccagc gcaatcccac
     2221 ggcgcggctc cgcgaagtgg cagacaccgt tgggatcacc gagcgggccg cccaacggat
     2281 cgtcaccgac ctcgttgacg gcgcgtacgt gacgaaggcc cgcgagggcc gacggaatcg
     2341 atacgagctt gaccccacag ttcggatgcg ccatccgtcg caggttgacc acgagatcgg
     2401 ggaacttctc gatctcctgc gactcgacga ccgaagcgcg agtagctccg ggactcggcc
     2461 tgctggggta ctcacggaac acagcggccc aggcacgtcg gcaagccgct ccgatcactc
     2521 tgaccctgga aacggttagg ccccggtgtg ttcccggggc cttggccgct tcaggcgcta
     2581 cgtcttcggt cgttaggtgt ggagggccct gccgccacgg cgatcgacga cgagccggta
     2641 gatgatgagc aggaggatcg acccgacgat tgccgttagc caggatgaga tgtcaaagaa
     2701 ctcgtccatc gggtccgcgc caaacagaac cccggcgagg aagccgccga tgagtgcacc
     2761 ggcgataccg atgagagccg tgacgatcca gccgccagga tcgtcgccgg gcatgatcgc
     2821 cttggcgatg ccacctgcga gcagacccag gatgatgaaa gcaatgattc cgatgtcctc
     2881 acctttcttc gaagtgcggc tgatattccc ggcggacagc cgaatcaaac gggcagggac
     2941 attggttcct cgagtggcat gtgcaaggca tataacggga accacatctg cggcgcggtc
     3001 gcggctcgcg ttactgctcg attgccttct gcgagcctgc gctgatcgtg atcttctttg
     3061 gccgctgctg gaccggcttg ggtacgtgca gctcgagcac gccgtcgtgg tactccgcct
     3121 cgatctgttc gccgtgaaca cccttcggga gagtcaggtt gcggacgaag ga
//
LOCUS       DASTXO010000279         5989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1380524, whole genome shotgun sequence.
ACCESSION   DASTXO010000279 DASTXO010000000
VERSION     DASTXO010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5989
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380524"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1379
                     /locus_tag="VFU96_05995"
     CDS             <1..1379
                     /locus_tag="VFU96_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5112851.1"
                     /translation="GMASAEDYDRVVAQSVETFDRWRLLPAPQRGQYVRRLGDALRES
                     IEPLGALVTLEVGKILPEGIGEVQEMVDICDFAVGLSRQLYGTTMPSERPNHRMYEHW
                     HPLGPIGVISAFNFPVAVWSWNSALAAVCGDTVVWKPSERTPLTAIAVTRIAQEVMEG
                     SGFEGVFNLVVGDGATVGNRMNQDTRLPLISATGSCNMGRIVGPAVAQRMGRTILELG
                     GNNAVIVMDDADLDLAVRATLFSAVGTAGQRCTSLRRLLVHRAIADKMVSRLTDAYKQ
                     VKIGDPLEAGTLMGPLVGERAIEQMMGALDIAREQGGRIVTGGNRLDRPGFFVEPTIA
                     VVPKEASITQQETFAPILWVIEFDTLEEAIEIQNGVTQGLSSAIFTESVRSAERFLSA
                     AGSDCGIANVNIGTSGAEIGGAFGGEKETGGGREAGSDAWKAYMRRQTTTINWGDDLP
                     LAQDIEFG"
     gene            1382..1966
                     /locus_tag="VFU96_06000"
     CDS             1382..1966
                     /locus_tag="VFU96_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017596652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5112852.1"
                     /translation="MDAADWDERYRDHEHLWSVAPNLFVADRLGGAPPGRGLDLAAGE
                     GRNAIWLASLGWEMTAVDFSEVAVDKGRAHSQAVDFVVADVRTWEPERTFDLVLIAYL
                     HLTPPDYETVVRRAEHWLASGGELFLIGHDVSNLTDGWGGPQYPELLWDVPEVLRWLD
                     GMAIIEAEVVNRPVDADDGRKYARDTLVRARQPG"
     gene            complement(1968..3119)
                     /locus_tag="VFU96_06005"
     CDS             complement(1968..3119)
                     /locus_tag="VFU96_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEU5112853.1"
                     /translation="MGFRTKQVHAGVTPDPTTGAILTPIHQSTTFVQDSVDEYMGKGY
                     SYSRGGNPTVRALEEKLTALEEGHDATAYASGMAATVAVFLGLLRAGDHVVIADVVYG
                     GTYRFADQFLTRFGVDVTFADASNPGAVEAAMRPETRLVFTESPANPTLKVTDLAAIS
                     EVTGSNGALHVTDNTVLTPYFQRPFELGANIVIHSTTKFLDGHNATLGGAVVVDRQEL
                     QESVSFARLSAGLVMSPMVAWLTLQGVKTLSERMDRQSANAMAIAQWLQDHPKVDYVN
                     YPGLTSHPQYELSSRQATGFGALVCFEVHGGAETGKKLMDAVELWSLAENLGAVESLI
                     THPVTMTHAAVPAEERVAAGITDGLVRLSVGLEDVEDLIADLESALDRV"
     gene            complement(3188..3823)
                     /locus_tag="VFU96_06010"
     CDS             complement(3188..3823)
                     /locus_tag="VFU96_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010475184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5112854.1"
                     /translation="MNVAVYPANCSEAILDGLLLAGATHVGAPDLVPEDRVVNDADIL
                     LVELGDQPIRRLREVNRIIEEIGLPVVLAISLDQLALIESASGISDFITTPVDPLELR
                     LRVHRLTREETADELVVFKDLELNTLNYQATLASKPIDLTFMEYELLRFLVQNPIRVW
                     SREQLLSKVWGYDYYGGARTVDVHVRRLRSKLGEERSSWITTVRSVGYRFG"
     gene            complement(3820..5145)
                     /locus_tag="VFU96_06015"
     CDS             complement(3820..5145)
                     /locus_tag="VFU96_06015"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEU5112855.1"
                     /translation="MNKQAEYVMRTVEERGIRFIRLWFTDVQGFLKSVSISPAELEVA
                     FEEGMTFDGSAIDGYARIQEADMLARPDPSTFGVLPWRTEQQVARMFCDIATPDGEPF
                     SGDPRIVLKRNLGRASEMGYNFYAAPELEFFYFRDSGPEPEVLDHGGYFDLTPLDVAQ
                     DYRRDTINALEQMGIPIEHSHHEVAPSQHEIIARYTDALTMADTIMTSRLTVKEVALK
                     HGIYATFMPKPLEEHDGSGMHLHLSLFEDDVNAFHEPGTPGGLSKIGQAFIAGILRHA
                     AEMTAITNQWVNSYKRLVDGFDAPIYISWARNNQSALVRVPTVKKGKPSSVRVEYRAA
                     DAACNPYLTLSVILAAGLAGIREGYELPPEISANIHLMTPAERAAAGAKRLPETLSEA
                     LDLMEGSELIAEALGEHVFDWFLRNKRKEWDRYQHHVSRFELESYLPVL"
     gene            complement(5174..>5989)
                     /locus_tag="VFU96_06020"
     CDS             complement(5174..>5989)
                     /locus_tag="VFU96_06020"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5112856.1"
                     /translation="VGVRVEEAVGRSVMTRLHGLWSLGTLIGSGLSALAVLVGLGLGL
                     QLLIVSVIGVGSAVYAGRLIPDATPRRREGQRSGRLAVGLMLAGAFAVVIEGAPFDWS
                     ALYLADFTGSSDALAGVGVIVFTAGMLIGRLAGDHVVDRFGSIRTLYAGIVLSVVAMF
                     VVVVPQQVVTSLIGFAFWGLGISVALPVLYKLAGSHRSFGEGSGLAALTVGTRLGFMV
                     APALIGVAATAWSLPAAMGLIVTLAAAASLLAVRLTIGRPVPTGDSLPGPGVS"
BASE COUNT         1082 a         1872 c         2003 g         1032 t
ORIGIN      
        1 tgggcatggc ctcggccgag gactacgacc gggtggtggc ccagtcggtg gagaccttcg
       61 atcgctggcg cctgctgccc gccccgcagc gtggacagta cgtgcgccgt cttggtgacg
      121 ctctgcggga gagcatcgag ccactgggcg ccttggtcac gctcgaagtc ggaaagatcc
      181 tccccgaggg aatcggcgaa gtgcaggaga tggtcgacat ctgtgatttc gccgtgggtc
      241 tctcccgtca gctctacggc acaaccatgc cttcggagcg gccaaatcac cggatgtacg
      301 agcattggca tccgctcggg ccgatcggag tgatctccgc gttcaacttc ccagtcgccg
      361 tttggtcgtg gaactccgcc ctggccgcag tctgcggcga cactgtggtg tggaagccct
      421 cggagcggac tccgctcacg gccatcgccg tgacccggat cgcccaggag gtgatggagg
      481 gcagtggctt cgagggcgtg ttcaacctcg tcgtcgggga tggggccacg gttggcaacc
      541 ggatgaatca ggacacccgg ctgccgctca tctccgccac ggggtcgtgc aacatggggc
      601 gcatcgtggg ccctgccgtc gcccagcgca tgggcaggac catcctcgag ctgggtggca
      661 acaacgccgt catcgtgatg gacgacgccg atctcgatct cgcagttcgg gccactctgt
      721 tctcggcagt cggcaccgca gggcagagat gtaccagtct tcgccgtctt ctcgttcacc
      781 gggcgatagc cgacaagatg gtgagccgtc tcaccgacgc ctacaagcag gtcaagatcg
      841 gcgacccgtt ggaggcgggg accctgatgg gcccactggt tggcgagcgc gccatcgagc
      901 agatgatggg agccctcgac atcgctcgcg agcagggggg caggatcgtc accgggggca
      961 accgcctcga tcggcccggg ttcttcgtcg agcccaccat tgccgtcgtg cccaaggagg
     1021 cgtcgatcac ccagcaggag acgtttgccc cgatcctttg ggtgatcgag ttcgacacgc
     1081 tcgaggaggc gatcgagata cagaacggtg tgacccaggg cctgtcatcc gcgatcttca
     1141 ccgagtcggt tcggagcgca gagagattcc tgagcgccgc aggctccgac tgtggcatcg
     1201 ccaacgtcaa catcgggacc tccggtgccg agatcggggg cgcctttggc ggggagaagg
     1261 aaacgggtgg cggccgagag gcagggtccg acgcatggaa ggcatacatg cgccggcaga
     1321 ccaccacgat caactggggc gacgacctgc ccctggccca ggacatcgag ttcggctgac
     1381 cttggacgcc gccgactggg atgagcggta tcgcgatcat gagcatctct ggtcggtagc
     1441 tcccaatctg ttcgtggccg accgactggg cggggcccca ccaggcaggg ggctcgatct
     1501 ggccgccgga gagggacgaa atgcgatctg gctggcctca ctgggttggg agatgactgc
     1561 cgtcgacttc tccgaggtag ctgtcgacaa gggcagggcg cactcgcaag cagtcgactt
     1621 cgtcgtcgcc gacgttcgga cgtgggagcc cgaacggact ttcgacctgg tcctcatcgc
     1681 ctatctgcat ctgaccccgc ccgactacga gaccgtggtg cgtcgggccg agcattggct
     1741 ggcgtccggc ggcgagctgt tcctgatcgg ccatgacgtc tccaacctca cagacggctg
     1801 gggtggcccg cagtacccgg agctcctatg ggatgtgccc gaggtgctcc gctggctcga
     1861 tggcatggcc atcatcgagg ccgaggtggt gaatcgtcct gtcgacgccg acgacggccg
     1921 caaatacgcg cgggacaccc tggtcagggc ccgccaaccg ggctgagtca gacccggtcg
     1981 agcgccgatt cgagatcggc gatcaggtcc tcgacatcct cgagcccaac cgagagccgg
     2041 acgagaccgt cggtgatgcc ggccgctacc cgctcctcag ccgggacagc ggcatgagtc
     2101 atcgtgaccg ggtgggtgat cagcgattcg acggcgccca agttctcggc gaggctccac
     2161 aactcgacgg cgtccatcaa tttcttgccg gtctccgcgc caccgtgcac ttcgaagcac
     2221 accagagctc cgaacccggt tgcttgccgc gacgagagct cgtattgggg gtgagaggtc
     2281 aggcccgggt aattgacgta atcgaccttg ggatgatctt ggagccactg cgcgatggcc
     2341 atcgcattgg ccgactgtcg atccattctc tcgctcaggg tcttgactcc ctgcaaagtc
     2401 agccatgcga ccatcggcga catcaccaag ccggcagaca gtcgcgcgaa agacacgctc
     2461 tcttgaagct cctgacggtc gacgaccacc gccccgccaa gggtggcgtt gtgaccatcg
     2521 aggaatttgg tcgtggaatg gatcacgatg ttggcgccga gttcgaacgg cctttggaaa
     2581 tagggggtga ggactgtgtt gtcggttacg tgcagggcgc cattcgaacc tgtgacttct
     2641 gagatagcgg ccagatccgt gaccttgaga gtcgggttgg ccgggctctc ggtgaacacg
     2701 agacgggtct ccgggcgcat cgccgcctcc actgcccccg gattcgaggc gtcggcgaag
     2761 gtgacgtcga ccccgaaccg ggtgaggaac tgatcggcga agcgataggt gccgccgtac
     2821 accacgtcgg cgatcaccac gtgatcacct gcccggagca gacccaggaa gactgcgacc
     2881 gtggccgcca tcccggaggc gtatgcggtg gcgtcgtgcc cttcctccaa cgcggtcagc
     2941 ttctcctcga gtgcgcgcac agtggggttg cctccccggc tgtaggagta ccccttgccc
     3001 atgtattcgt ccaccgagtc ctggacgaaa gtcgtcgact ggtggatggg ggtgaggatc
     3061 gcgccggtgg tcgggtcggg agtgactccg gcgtgtacct gtttggtgcg aaaacccatg
     3121 tgccaattgt ggtcggtccc gacatgttgg ggcgaacctg aggcgagcgg ctgatcttct
     3181 ccgggagtca gccgaagcgg tagcccaccg agcgaacggt ggttatccag gatgagcgct
     3241 cttcgccgag cttggagcgg aggcggcgaa cgtgcacgtc gacggttcgg gcgccgccgt
     3301 agtagtcgta gccccagacc ttggagagga gctgttcccg ggaccagacc cggatcgggt
     3361 tctggaccag aaagcggagc agctcgtact ccatgaacgt gaggtcgatg ggcttggaag
     3421 ccagggtggc ctgataattg agcgtgttca actccaggtc cttgaacacc accagctcgt
     3481 cagccgtctc ctccctggtc agtcgatgga cacgcaacct gagctcgagt gggtcgaccg
     3541 gggtggttat gaaatcggag ataccgctcg ccgactcgat gagcgccagc tgatcgaggg
     3601 agatggcgag gaccaccgga agcccgatct cttcgatgat ccgattcacc tcccgcagac
     3661 gccggatcgg ctgatcgccg agctcgacga ggaggatgtc ggcgtcgttg accacgcggt
     3721 cctcgggaac gagatcgggg gcacccacgt gggtcgcccc ggccagcagc aacccgtcga
     3781 ggatggcttc cgagcagttg gctggataga ccgcgacgtt cacaggaccg gcagatagct
     3841 ctctaactcg aaacgtgaga cgtgatgctg gtagcggtcc cactccttgc gcttgttgcg
     3901 gaggaaccag tcgaaaacgt gctcaccgag cgcctccgcg atcagttccg acccctccat
     3961 gaggtcgagg gcctcggaca gtgtctccgg aagccgcttc gctccggcgg cagcgcgctc
     4021 ggccggtgtc atgagatgga tgttggcgga gatctcgggg ggcagctcgt agccctcccg
     4081 gattccggcc agcccggcgg ccaggatcac cgacagggtc aggtaggggt tgcaggcggc
     4141 gtcggcggcc cgatactcca cccggacact cgacggcttg cccttcttga cggtcgggac
     4201 cctcaccagc gccgactggt tgttgcgggc ccaagagatg tagatcgggg cgtcgaagcc
     4261 gtcgacgagc cgtttgtagg aattgaccca ctgattggtg atcgcagtca tctcggcggc
     4321 gtgacgaagg atgccggcaa tgaatgcctg gccgatcttc gacagccctc ccggcgtccc
     4381 cggctcgtgg aaggcgttga cgtcgtcctc gaagagcgac aggtgcaggt gcatgcccga
     4441 gccgtcgtgc tcctccagtg gctttggcat gaacgtcgca tatatcccgt gcttcagggc
     4501 cacttccttg accgtcaagc gcgaggtcat gatggtgtcg gccatggtca gcgcatcggt
     4561 gtaacgagcg atgatctcat gctggctggg tgccacctcg tgatgagagt gctcgatcgg
     4621 gatgcccatc tgctcgaggg cattgatcgt gtctctcctg tagtcctggg ccacgtcgag
     4681 cggggtcagg tcgaaatagc cgccatgatc gagcacctcc ggctcgggcc ctgagtccct
     4741 gaagtagaag aactccagct cgggggcggc gtagaagttg taccccatct cggaggctct
     4801 gcccaggttt cgtttgagga cgatgcgggg atcgcccgag aacggctcgc cgtccggggt
     4861 ggcgatgtcg caaaacatcc gggccacctg ctgctctgtg cgccagggca ggaccccaaa
     4921 ggtcgacggg tccggtctgg ccagcatgtc ggcctcctgg atgcgggcgt agccgtcgat
     4981 ggccgaaccg tcgaaggtca ttccctcctc gaaggccacc tccagttcgg cagggctgat
     5041 cgagaccgac ttgaggaaac cctggacgtc ggtgaaccag agcctgatga atctgatccc
     5101 gcgctcttcg acggtacgca tgacatattc ggcctgcttg ttcatgccgg ttcccgggtc
     5161 ggttgagcac acattatgag acccccggtc cgggaaggga atccccagtc ggcacgggcc
     5221 ggccgatggt gagccggacc gcgagaagcg acgcggcagc agcgagcgtg acgatcaggc
     5281 ccattgcggc cggcaggctc catgcggtgg ccgcgacacc gatcagcgca ggggcgacca
     5341 tgaatccgag tcgggtgccg acggtcagcg ccgccagacc tgagccttcg ccaaaggagc
     5401 gatgggagcc cgccaacttg tacaggacgg gcagcgcgac cgagatgccg agcccccaga
     5461 atgcgaatcc gatcaatgag gtcacgactt gttgcgggac gaccacgacg aacatcgcga
     5521 caacggacag cacgatgccg gcatagaggg tgcggatgga gccgaatcga tcgacgacat
     5581 gatcccccgc cagtcgcccg atcagcatcc ccgcagtgaa gacgatgaca cccacgccgg
     5641 cgagggcgtc actactgccg gtgaagtcgg ccagatacag ggcgctccaa tcgaacggcg
     5701 ctccctcgat gacgacggcg aacgcgcctg ccagcatcag cccaacggca aggcggcccg
     5761 accgctggcc ctcgcgccga cgtggggtcg catccgggat cagacgtccg gcgtacacag
     5821 ctgatcccac gccgattacg gagacgatca ggagctggag gccgagacct aggccgacga
     5881 ggacggccag agcgctcagc ccggacccga tcagggtgcc cagactccac agcccgtgga
     5941 gtctcgtcat aaccgaccgg cccaccgcct cctccacgcg gacgccgac
//
LOCUS       DASTXO010000280         3907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_901669, whole genome shotgun sequence.
ACCESSION   DASTXO010000280 DASTXO010000000
VERSION     DASTXO010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901669"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..758)
                     /locus_tag="VFU96_06025"
     CDS             complement(126..758)
                     /locus_tag="VFU96_06025"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU5112857.1"
                     /translation="MVRDLAYFISGDSGPGPGVMVLHSFWGLTPSVKSLCDDLADHGY
                     TVLAPDINFGELPASEQEALDHLGKADPNRLASLVLSSAKLLHEKSTEGPIAIVGFGM
                     GGSLALWASVRLADLVSAAVSFYGSQQIDFAGSKAGYVIHLAEEDEYVSDDEAAFMEA
                     TMRLENLEVEVSRYPGTRHGFAEPDGDTFDPAAFDLAWERTLDFLQDRLG"
     gene            792..2450
                     /locus_tag="VFU96_06030"
     CDS             792..2450
                     /locus_tag="VFU96_06030"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5112858.1"
                     /translation="MTSAVAPLRYGSFRTLWVASMLSAFGTFIQSLSSSQLMFDLTGS
                     NTWVGLMVASTSLPLLFFALAAGALADMFDRAKLMLVAQGIMGGSALAMAILTGTGAI
                     TPGLLLGLGFILGIGVALNLPTWQALLPDMVPRGLIASAVALQSAAFNVARAVGPAMG
                     GFIYAAFGAAVGFGINALTYGFVIAGLLMVRHTFEKRQRDPAPLGTAILLGIRYARFT
                     PVFRRLLLLVSLFAITSSVVQSVLPGHNEAIGGTASTLGILLGAMGVGALVATVVRQR
                     FIDRFHRWAPMVTIGLFGLVGIGLGLSTSIATAAFFMFFSGAFWVLTLTNLNATAQLM
                     APEWIRGRAMSLYSFSFLGIMPLGSILSGVLADAVGTGPAIVILCSASVLLGALVPMF
                     HLPSLDQIESPEFTPDRPGPLHEEETVEGGPVLILNTWKINLADFEEFTAVMNLVRLV
                     RLRTGAYRWRLLRNVSDPTRLTELFATGSWEEHLAQHRRIDDASADIIARARSFDRDG
                     GPVTRHLISVDVEDPPNFAELVSTHEVMHQTDGSIPTMADQDVD"
     gene            complement(2463..3158)
                     /locus_tag="VFU96_06035"
     CDS             complement(2463..3158)
                     /locus_tag="VFU96_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112859.1"
                     /translation="MDGSRVGATLDDAENAVTAGDPVAPTGFWKAVDVVKRDPVLIEQ
                     YADRIARIDRAAFKTWALVAVPIWLGNVLIIGATLIGLALVGWAYYLEGTGAALVFLL
                     GFGIILVTTHGLTHLLVGSLMGMRFTHWFVGKLSQPQPGVKLDYATYLRAPAASRAWM
                     HASGAIVTKLMPFIFLGAAIAAGLPTWVVWALVVIGLVEIVIDIVWSTQKSDWKKFKR
                     EMEFASPAGSSTG"
     gene            3222..3740
                     /locus_tag="VFU96_06040"
     CDS             3222..3740
                     /locus_tag="VFU96_06040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU5112860.1"
                     /translation="MDDLTAALARDLDGAFSTLVETAGPGLYWGLRRLCGDHQEAEDL
                     TQETFIRAYRALGEFDRRRFEELRLSPWLWTIALNLGRNHLRNRSRRPTLVEMRSNPG
                     VDDPEPPDLAAWDARLSALPVNQRRAVVLRHVVGLGIGEIVEATGRPEGTVKTDIHRG
                     LDRLRRVMEQEQ"
BASE COUNT          668 a         1288 c         1234 g          717 t
ORIGIN      
        1 ggatgagggc ccatctcggc cagccgagcg agtaggcggc ggattccctg gtcattccaa
       61 actccgggtc gactcaggtg gaagctagcc agcggccatg acggacgggc actcgccgag
      121 ccatgtcagc ccaaccggtc ctgcaggaag tccagagtgc gctcccaagc gaggtcgaag
      181 gcggccggat cgaacgtgtc accgtctggc tcggcgaacc cgtggcgagt gcccgggtat
      241 ctggagacct cgacctcgag gttctcgaga cgcatggtcg cctccatgaa ggctgcttca
      301 tcatcagaca cgtactcgtc ttcctcggcg agatggatca cataccccgc tttggacccg
      361 gcaaagtcga tctgctggct gccatagaac gagaccgcgg cgctgaccag gtcggcgagg
      421 cggaccgatg cccagagggc gagcgatccg cccatgccga agccaacgat tgcgatcggg
      481 ccttcggtcg acttctcgtg gaggagcttg gccgaggaca acacgagtga ggcgaggcga
      541 ttggggtcgg ccttgcccag atgatcgagc gcctcctgct cagaagccgg caactcgccg
      601 aaattgatgt ccggggcgag gaccgtgtag ccgtgatcgg cgagatcatc acagaggctt
      661 ttcaccgacg gcgtcagacc ccagaaggag tggaggacca tcacccccgg tccggggccc
      721 gagtcaccgg agatgaagta ggcgaggtcg cgtaccacgt cgcgaaccta ccgggcctac
      781 gctggtagca gatgacctcg gcggtcgccc ctctccgtta tggctctttc cgcacgctct
      841 gggtggcgtc gatgctgtcc gcgttcggga ccttcatcca gagcctttcc tcatcccaat
      901 tgatgttcga cctcaccggg tccaacacat gggtgggcct catggtggct tcgacctccc
      961 tcccgctgct gttcttcgcg ctggcggccg gcgccctcgc cgacatgttc gaccgggcca
     1021 agttgatgct ggtggcccaa ggaatcatgg gtggctcggc tctagcgatg gcgatcctca
     1081 ccgggaccgg cgcaatcacg ccagggttgc tgctcgggct cggcttcatc ctcggcatcg
     1141 gagtggcgct gaatctcccc acctggcagg cgttgctccc cgacatggtg ccgcgcggcc
     1201 tcatcgcgtc ggccgttgcc ctccagtcgg cggctttcaa cgtggcgcgg gcagtggggc
     1261 cggcaatggg tggattcatc tacgcggctt tcggcgccgc cgtcggcttc gggatcaatg
     1321 ccctcaccta cggcttcgtg attgcggggc tcctcatggt gagacacacc ttcgagaaga
     1381 gacagcgaga cccggcgccg ttgggcaccg cgatcctgct cggcattcgc tacgcccggt
     1441 tcaccccggt gttccgccgg ctcctcctcc tggtctcgtt gtttgccatc acctcatcgg
     1501 tcgtgcagtc ggtgctgcct gggcacaacg aagccatcgg cggcactgcc tccactctcg
     1561 gcatcctgct cggtgccatg ggggtcggcg ccctcgtggc gacggtggtg cgacagcgct
     1621 tcatagaccg gttccaccga tgggccccga tggtgacgat cggcctgttc ggcctggtcg
     1681 ggatcggcct cgggctctcc accagcatcg ccaccgccgc cttcttcatg ttcttctccg
     1741 gggctttctg ggtgctcaca ctgaccaacc tcaatgccac tgcacagctg atggcgccgg
     1801 agtggatccg gggacgggcc atgtcgctgt acagcttctc tttcctcggc atcatgccgc
     1861 tcgggtcgat cctctctggc gtcctggccg acgcagtggg aaccgggccg gcgatcgtca
     1921 tcctctgctc ggcttcggtg ctgctcggtg cgctcgtccc gatgttccat ttgcccagtc
     1981 tcgatcagat cgagtccccc gagttcactc cggatcgccc cggccccctg cacgaggaag
     2041 agaccgtgga aggcggcccg gtcttgatcc tgaacacctg gaagatcaat cttgccgact
     2101 tcgaggaatt caccgcagtg atgaacctgg tcaggctggt ccggcttcgc acgggtgcct
     2161 accggtggcg gctgttgcga aatgtcagcg acccgacgcg cctcaccgag ctgttcgcaa
     2221 ccgggtcgtg ggaggagcac ctggctcagc atcgccgcat cgacgacgcc tcggcagaca
     2281 tcatcgcccg ggctaggtcg ttcgatcgcg acggcggccc ggtcacgaga catctgatct
     2341 cggtcgatgt cgaagaccca cccaacttcg ccgaactggt cagcacccac gaggtgatgc
     2401 accagacgga cggatcgata ccgacgatgg cggatcagga tgtggactga gtgagccccg
     2461 gcctacccgg tactgctccc tgccggagaa gcgaattcca tctcccgctt gaacttcttc
     2521 caatccgact tctgtgtgga ccagacgatg tcgatgacga tctcgacgag gccgatgacg
     2581 accagggccc ataccaccca ggtggggagc ccggcggcga tggccgcgcc gaggaagatg
     2641 aatggcatca gtttggtgac gatggcgccc gacgcgtgca tccaagccct cgatgccgcc
     2701 ggagcccgca gataggtcgc gtagtcgagc ttcactccgg gttggggctg gctcagtttc
     2761 cccacgaacc agtgggtgaa cctcattccc atcaatgacc cgaccagcag atgggtcaac
     2821 ccatgggtcg tcaccaggat gatcccgaac ccgagcagga agacgagggc cgcgccggtc
     2881 ccctccaggt agtaggccca accaaccagg gccaatccga tgagggtggc cccgatgatc
     2941 aggacgttcc cgagccagat cggcaccgcc acaagcgccc acgtcttgaa cgcggcccga
     3001 tcgatccgag cgatcctgtc tgcgtattgc tcgatgagga caggatcccg cttgacgacg
     3061 tcgactgcct tccagaaacc ggtcggggct accgggtcgc cggccgtgac cgcattctcg
     3121 gcatcgtcca gcgttgcccc gacccgtgag ccgtccatga ccctcatcta agtacggtgc
     3181 gaaggtgcaa cccgtgcatc tcacctggtg ttctctttgc gatggatgat ctgaccgcag
     3241 cattggccag agacctggac ggcgccttct ccacgctggt ggagacggcc ggccccggcc
     3301 tctactgggg gctgcgtcgg ctctgcggcg atcaccagga ggccgaagac ctcacccagg
     3361 agaccttcat ccgcgcttat cgggcgctcg gtgagttcga ccggcggcgg ttcgaggagc
     3421 tcaggttgtc gccatggcta tggaccatcg cgctgaacct ggggcgaaac cacctccgca
     3481 accgttcgag gcggcccacc ctggtggaga tgcgctcgaa cccgggggtc gacgaccctg
     3541 agccacccga cctcgcagcc tgggacgccc gactctcggc tctgccggtc aaccaacggc
     3601 gagcggtggt cctgcgccat gtcgtcggac tgggcatcgg agagatcgtg gaggccacag
     3661 ggcgtcccga agggacggtc aagaccgata tccatcgtgg actggatcgt ctgagacgag
     3721 tcatggagca agaacaatga acatcgagga ccaactttcg ggactgaccg gaggtctgcc
     3781 tgacggcatc agtgaaggcg tcgcgctggg caccggcatg gcggacggct acgacctcta
     3841 cccctcaccc gtgggcgagg tggtagtgac cttcaacata gacggtgtct cgtctcttga
     3901 cctggcc
//
LOCUS       DASTXO010000281         2511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1233414, whole genome shotgun sequence.
ACCESSION   DASTXO010000281 DASTXO010000000
VERSION     DASTXO010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233414"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..156)
                     /locus_tag="VFU96_06045"
     CDS             complement(<1..156)
                     /locus_tag="VFU96_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005575429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU5112861.1"
                     /translation="MSDRDVVLVTGGRGFIGAWVVKTLLADGRDVVALDRDPDRTRLA
                     QVLEEDDV"
     gene            219..701
                     /locus_tag="VFU96_06050"
     CDS             219..701
                     /locus_tag="VFU96_06050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEU5112862.1"
                     /translation="MPVTLSPSGTRGTAIPGFAKALMKAGAGVGNLLFSMMGDRMKVQ
                     GRPLVLLTTVGAKSGKERQTLLGRFDDTENPGAGLVVGSNGGAARHPGWCHNLAANPE
                     QVWATIEKHKTRVRPDSLDGSTYQEAWDRIVSLAPGYGSYLDKTDRHIPIIRLTPATE
                     "
     gene            757..1314
                     /locus_tag="VFU96_06055"
     CDS             757..1314
                     /locus_tag="VFU96_06055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5112863.1"
                     /translation="MSERNALELTESPGIRFDHLFEANYNAIYRYCARRLGRSDAEDA
                     AAEVFTVAWRRLDDMPDGDAMRAWLFGVAHRVVGGQYRSRERRSRLAVRLEQASDSNP
                     VDFEPPGDIDALLTALHGLSRTDQELLRLSSWDGLTRNEIAQVLGIKENAVDQRLHRA
                     RNRLKVRFDRLSNEAASLSHKEAPA"
     gene            1311..>2511
                     /locus_tag="VFU96_06060"
     CDS             1311..>2511
                     /locus_tag="VFU96_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112864.1"
                     /translation="MIELLERADPANDLVADRGRLRGKVDERIGLSAPLRPATRRMKK
                     PWSIAIVSFVAVLAVAVPVALRTDPPSVFAPDLDGLESHPGVQAAVPLASGGLQAMNA
                     DGGTIWVMTTLLNVLQEVSAESGEIVATFQVDVRVEGLVIGGGRVWLISPDDGGQVLR
                     FDPESGAVDLTIPVGGVPGWAAWLGDSLWVGRDQTDVIRISTEGEIIDTVPGELKGGE
                     GLGYLWVNDPATDLISSLSEEAQLGEIVIPTERGLETMSGAGIRQVAEADGRLFLLDG
                     DHPWGTNLNVFDPESGEFGAFASLTFGLLDLIEFDGYLWVTSSTDHLLIRIDPASGEQ
                     HRYPLPGKPGGLTVADDALWVTLYHPGALIRLDTSSDLTESSPIVADDWNRFPHRLLC
                     TGTGATGG"
BASE COUNT          448 a          811 c          818 g          434 t
ORIGIN      
        1 cacatcgtct tcttcgagga cctgtgccag acgcgtccga tcggggtccc gatcgagagc
       61 aaccacgtcg cggccgtcgg cgaggagagt cttgaccacc caggcgccga tgaagccccg
      121 ccctccggtg accagtacca catcccgatc gctcatgccc gcgatgctaa gtgggcgggc
      181 cgtcgggatc ggataggctc cgccaccgaa ggagcatctt gcccgtcaca ctctcacctt
      241 ccggaacccg gggcaccgcg atccccggct tcgccaaggc cctgatgaag gctggggcag
      301 gtgtcggcaa ccttctcttc agcatgatgg gcgatcgaat gaaggtgcaa ggccggccgc
      361 tcgtcttgct gaccacggtg ggtgccaaga gcgggaagga gagacaaacg ttgctcggcc
      421 ggttcgacga cacggaaaac cccggcgcgg ggctcgtggt cggctccaac ggaggcgcgg
      481 cccggcaccc gggctggtgt cacaatctcg ccgccaatcc cgaacaggtt tgggcgacga
      541 tcgagaagca caagaccaga gtccgcccgg attcactcga cgggagcaca taccaggagg
      601 cgtgggatcg gatcgtgtcg ttggcgcccg gatacgggtc ctatctcgac aagaccgatc
      661 ggcatatccc gatcatccgc ctgacgccgg cgaccgagta gccgaggtgt aagcggagcg
      721 gctcatccgg gcacataaca tgccagaaag gcagatgtgt ccgagagaaa tgccctcgag
      781 ctgaccgaat ccccgggcat tcgtttcgac catctgttcg aggccaacta caacgccatc
      841 taccgatact gcgctcgccg cctcggacgc tccgacgccg aggacgccgc agccgaggtg
      901 ttcaccgtcg cctggcgccg tctcgatgac atgcccgatg gtgatgcgat gagagcgtgg
      961 ttgttcgggg tcgctcatcg agtggtgggg ggtcagtatc gaagcaggga gcgtcgatcc
     1021 cggctcgcgg ttcgtctcga gcaggcttcc gactcaaacc ccgtcgattt cgagccccca
     1081 ggtgacatcg acgctctgct caccgcactc cacgggctga gccgaaccga ccaggagttg
     1141 cttcggctgt cgtcctggga cggcttgacc cgcaacgaga tcgcccaggt gctgggcatc
     1201 aaggagaacg ccgtcgacca gcggcttcat cgggcacgga accggttgaa ggtcagattc
     1261 gaccgtctca gcaacgaggc ggcatcgctc tcgcacaagg aggcccccgc atgatcgagc
     1321 tactggagcg cgccgatccg gccaacgacc tcgtagcgga caggggtcgg ctccggggca
     1381 aggtcgacga gcgtatcggc ctctccgcgc cgctgcggcc ggccacccga aggatgaaga
     1441 aaccctggtc gatcgcgatc gtctcatttg tggctgtgct cgcggtcgcc gttccggtgg
     1501 ccctccgcac cgatcctccg tcggtcttcg ctcccgatct cgacggcctc gagtcgcatc
     1561 ccggcgttca agcggcagtg ccgctggcgt cggggggcct tcaggccatg aacgcggacg
     1621 gcggcacgat ctgggtgatg accaccctgc tgaacgtcct ccaggaagtc tcggccgaat
     1681 ctggagagat tgtggcgacc tttcaggtcg acgtccgagt cgagggcttg gtgatcggag
     1741 gcggtcgagt ctggctgatc agccccgacg atggcggcca ggtgcttcgt ttcgaccccg
     1801 agagcggggc ggtggacctg accatccccg ttggaggagt gccggggtgg gcggcctggc
     1861 tgggggacag tctgtgggtt ggccgtgatc agaccgacgt gatccgaatc tcgaccgagg
     1921 gcgagatcat cgacacagtt ccgggcgagc tcaaaggtgg cgaaggcttg ggctatctct
     1981 gggtgaacga tcccgccacg gacctgatct ccagcctttc cgaagaagcg cagctcggcg
     2041 aaatcgtgat cccgaccgaa cgcggcctgg agaccatgag cggagctggg attcgccagg
     2101 tcgccgaagc ggacggccgt ctcttcttgc tggatggcga tcatccctgg ggtaccaatc
     2161 tgaacgtctt cgaccctgag tcgggcgagt tcggcgcgtt cgcgagcctc actttcgggc
     2221 ttctcgacct catcgaattc gacggctatc tgtgggtgac ctccagcacc gatcatctgc
     2281 tgatccggat cgacccagcg agcggggagc aacacaggta cccgctgccc ggcaagcctg
     2341 gtggtctgac cgtggctgat gacgctctct gggtgaccct ctatcacccc ggagccctga
     2401 tccggctcga caccagctct gatctcaccg agtcgagccc gatcgtggcc gacgactgga
     2461 accgattccc gcatcgactg ctctgcaccg gaaccggcgc aaccggtggc c
//
LOCUS       DASTXO010000282         2384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_827996, whole genome shotgun sequence.
ACCESSION   DASTXO010000282 DASTXO010000000
VERSION     DASTXO010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827996"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..871)
                     /locus_tag="VFU96_06065"
     CDS             complement(119..871)
                     /locus_tag="VFU96_06065"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU5112865.1"
                     /translation="MCYNGDALPPVDAIEGGASGSDLVLASVDGNRFAAFAATSAESK
                     GPGVVILPDVRGLHHFYEELALRLAESGYDAVAIDYFGRTAGVGKRDDEFAYKEHVPR
                     TTLKGVTADTAAAVDLLRGSDPQRPIFTLGFCFGGSNSWHQAASGHGLAGAIGFYGNP
                     IRPPKGPIEVVGDFECPILALMGGDDPGIPNEVVEGFRQAMDNAGVENEVVVYSDAPH
                     SFFDRQQERFSRESADAWSRVLEFIALHGTKA"
     gene            914..2032
                     /locus_tag="VFU96_06070"
     CDS             914..2032
                     /locus_tag="VFU96_06070"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEU5112866.1"
                     /translation="MTATNSELALTWDQALAWRLGRQLLDSPGKDPVQVTSTTAGVQA
                     QVASSAAQAIAIRSKRVPDIESLLWEKRSLVKTWAMRGTLHLLPASEMGTWIAALRQR
                     PWKITPAWEKYHGITKSQLDSLTAAIPSVMSAEPMTREELTAAIVAETRDRGLGEALA
                     SGWSQVLKPAANQGLLAQGPPRGRNVTFVDPGAWLGNAIAESTAGKAMQIVIEKFLDA
                     NGPATTADFARWFGVDPKTGRVLMTPVIEKLVSVEIEGYQAWLTSRGARAAAKTSPAH
                     GAYLLPGFDPYTLAPISHREHIIPAGKVDEVSRAAGWIAPVVLVDGRIVGTWESENGA
                     IALQPFGKIPKKTVSMLEDHIEQRYHGLLGKSIAVTVP"
     gene            2064..>2384
                     /locus_tag="VFU96_06075"
     CDS             2064..>2384
                     /locus_tag="VFU96_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112867.1"
                     /translation="MSDPRRRRLAWALWVVTILASVVEVALLFANADLPVADAVDAVD
                     PWLQAMEDLAFVAVGTLGLRIILSQPRNAVGWWIMTAGLAFPLEGLTAEFTRFGIDRW
                     GSTPI"
BASE COUNT          440 a          766 c          779 g          399 t
ORIGIN      
        1 atcctcagga gctccgggta gtccgctccg attcgctcga gcagcccggt ggatccttcg
       61 atgtcgagga agagaaaggt aaccgttccg ctcgggagcg gggcaactcc cggcatgatc
      121 aagccttcgt gccgtggaga gcaatgaact cgaggacgcg gctccacgca tccgccgact
      181 ccctcgagaa ccgctcctgc tgccggtcga agaacgagtg gggggcatcg gaatagacga
      241 caacctcatt ctcgactccg gcattgtcca tggcctgccg gaaaccctcg accacctcgt
      301 tcggaatccc gggatcgtcc ccacccataa gggcgaggat cgggcactcg aaatcgccca
      361 ccacctcgat aggccccttc gggggtcgaa tcgggttccc gtagaacccg atggcaccag
      421 cgagaccgtg cccggaggca gcctgatgcc aggagttgga tcccccgaag cagaaaccga
      481 gtgtgaagat cggccgttgc gggtcggagc cgcggaggag gtccacggcg gccgcggtgt
      541 cggcggtgac acctttgagc gtggtccgtg ggacgtgctc cttgtaggcg aattcgtcat
      601 ccctcttgcc tactccggcg gtccgtccga agtaatcgat cgccaccgcg tcatagccac
      661 tttcagcgag gcggagggcc aactcctcgt agaaatggtg cagcccacgc acgtcgggaa
      721 ggatgaccac gcccgggccc ttgctctcgg cgctggtagc cgcgaacgcg gcgaaccggt
      781 ttccgtcgac cgaggccagc acgaggtcgc tgcccgaggc gccaccctcg atcgcatcga
      841 caggtggtag ggcatcaccg ttgtagcaca tggggtctcc ggttagaagc ggcaggcatg
      901 aaactacccg acgatgactg cgaccaactc ggagctggcc ctcacctggg accaggcact
      961 cgcctggcgc ctgggtcgac agcttctcga ttcacctgga aaggacccgg tgcaggtcac
     1021 ctcgacgaca gctggcgtcc aggcgcaggt tgcctcctcg gccgcccagg caatcgccat
     1081 caggagcaag cgggtacccg acatcgaaag tctgttgtgg gagaagcgga gcctcgtgaa
     1141 gacctgggcg atgaggggga cgcttcacct cctgcccgca tcggagatgg gcacctggat
     1201 cgccgccctc cgacaacggc cgtggaagat caccccggcc tgggagaaat accacgggat
     1261 aaccaagtct cagctcgact ccctgaccgc ggctatcccg tcggtcatgt ccgctgagcc
     1321 gatgacacgt gaggagttga ccgcggcgat cgtggccgag acccgtgatc gcggactggg
     1381 tgaggcgctt gcgtccggtt ggagccaggt gctcaaaccg gctgccaacc agggtttgct
     1441 agcgcaaggc ccaccgcggg ggcgcaatgt caccttcgtc gatcccggcg cctggctcgg
     1501 taacgcaatc gccgagagca cggccgggaa ggccatgcag atcgtcatcg agaagttcct
     1561 ggacgcaaac ggcccggcca cgactgccga cttcgctcgt tggttcggag tcgacccgaa
     1621 gaccggccga gtgcttatga cccccgttat cgagaagctg gtgtccgtgg agatcgaggg
     1681 atatcaggca tggctgacct cgcggggcgc cagggcggcg gcgaagacga gcccggcaca
     1741 tggcgcctat ctccttcccg gattcgaccc ctacaccctg gccccgatca gtcatcgcga
     1801 gcacatcatc cccgcaggca aggtcgatga ggtatcccgg gcggcgggct ggatcgcgcc
     1861 ggtggtcctg gtcgatgggc ggatcgtcgg gacctgggag agcgagaacg gggcgatcgc
     1921 cctccagcca ttcgggaaga tcccgaagaa gacggtttcg atgctcgaag accacatcga
     1981 acagcgatat cacggtcttc tcggcaaatc gattgccgta acggtcccgt gacgagcccg
     2041 acccatgatt tcgtggatga agaatgagtg acccccgccg gcgacggctg gcctgggccc
     2101 tttgggtggt gacgatcctc gcctccgtgg tcgaggtagc cctgctcttc gcgaatgccg
     2161 acctccccgt cgcggatgcg gtggacgcgg tggacccatg gctccaagcc atggaggacc
     2221 tcgccttcgt tgcggtggga acgctcgggt tgcgcatcat cctgagtcag ccccggaacg
     2281 ccgtcgggtg gtggatcatg accgccggac tcgccttccc tttggagggc ctgacagccg
     2341 agttcacccg gttcgggata gacaggtggg gctcgacccc gatc
//
LOCUS       DASTXO010000283         3175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1196751, whole genome shotgun sequence.
ACCESSION   DASTXO010000283 DASTXO010000000
VERSION     DASTXO010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196751"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VFU96_06080"
     CDS             complement(<1..405)
                     /locus_tag="VFU96_06080"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEU5112868.1"
                     /translation="MNEIPKATVVRLPRYLRLLEDLDSSKDVVSSEELATAAGANAAN
                     VRRDLSHIDFHGVRGIGYSVAELKVRIRQELGIAERQRVAIVGAGNLGRALANYAGLS
                     RRGFDVVALYDTDVRKIGSKVGSVIIQDLEVLD"
     gene            477..1712
                     /locus_tag="VFU96_06085"
     CDS             477..1712
                     /locus_tag="VFU96_06085"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HEU5112869.1"
                     /translation="MSSPSVLVVGGGPAGSAAAYWLAREGHDVTLVEKKEYPRDKTCG
                     DGLTPRAVLQLQDMGFEFDVPEFHRITGLRSYAGDEYVELKWPEHSRFPSWGGVMRRS
                     DLDSQVATLAEKQGVTIMEKTTATPVIEDGFLTGVTLDHDGSQDVVTPMVSVIADGSM
                     NRFGRELGNERRRDYPMGMAARGYYASPRSKDPFLESQLDLRDSTGATMPGYGWVFPL
                     GDGTVNVGVGLLSTFTRWKHVNTTHMMADYVATAPDYWELSEESKLTNPVGGKLTMSF
                     SKGPLVGANWITIGDAAGAINPWNGEGISYAYETGRIAAEHVGRALGTADFGLLRRYP
                     QQLEDEYGLYYKMARIFVKLIGHPPVMRTLAHTGLNSRSLMEWTLKVMANLLDEDDKH
                     MGERTYDALAAVVRTLPTS"
     gene            1709..2374
                     /locus_tag="VFU96_06090"
     CDS             1709..2374
                     /locus_tag="VFU96_06090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEU5112870.1"
                     /translation="MKPELLVFDVNETLLDMSPLRIDFEDVFGDADALGEWFARLLHG
                     SLVSNQLGDYRAFGVIGVEALLLVAERRGIDLDEATATELVTRLAILPAHLDVIPALE
                     QLLDAGFRTAALTNGSTKAANVQIENAGLHTFIQRVISVEEVGRFKPDQATYRHAAQV
                     MDVHVSKSMLISTHDWDVAGAVGAGASAAFVRRPGYLWTLPDPMPEVVGGDLKEIADQ
                     LIG"
     gene            complement(2375..>3175)
                     /locus_tag="VFU96_06095"
     CDS             complement(2375..>3175)
                     /locus_tag="VFU96_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5112871.1"
                     /translation="IGTGVILAPLHHPLRLAEDAATVSLLSGGRFTLGLGLGWSEVEF
                     DRLGARIDQRGRAMEEILTILPKAWTGQPFTHEGRVYGCDEVAVRPAPPEPVPILIGG
                     GAEPAIRRAARLSDGIYSNATTNGFLRQLEWVRDECDRIGRDPSELRIVHYSVILPGD
                     SADDAWARYTEHLWQMTWKYSDMEASATRSGPPPAAPELTEADRGALLRRATIAGSSD
                     EIVDSLLDLRDKAGIAVEFAARSYFPTLDYDSQVELMAHLAEEVAPRI"
BASE COUNT          542 a         1029 c         1033 g          571 t
ORIGIN      
        1 gtcgagaacc tcgagatctt ggatgatgac actgcccacc ttggaaccga tcttccggac
       61 gtcggtgtcg tacagggcca cgacatcgaa gccgcggcgg ctgagcccgg cgtagttagc
      121 cagggcccga cccaggttcc ctgctccgac tatcgccacc cgttgccgct cggcgatccc
      181 gagctcctgg cgaatccgca ccttgagctc ggcgaccgaa taaccgattc ctcgcacccc
      241 gtggaagtcg atatgggaga ggtcccggcg gacgttggcc gcgttggcgc ctgctgccgt
      301 cgccagctcc tccgagctga ccacgtcctt cgatgaatcc aggtcttcca gcagccgcag
      361 atagcggggc aggcggacga cggtggcctt tgggatttca ttcacaagct cacgataaac
      421 cggcgcggag tcttcggaat ctccgcttcc ggcgccggtc tctaacctct cagccgatgt
      481 cttctccctc cgtcctggtc gtgggtggcg gccccgccgg ctcagccgcc gcttattggc
      541 tggcccggga gggccacgac gtgactctgg tcgagaagaa ggagtacccc agggacaaga
      601 cctgtggtga tgggctgacc ccgagggcgg tcctccagct ccaggacatg ggtttcgagt
      661 tcgacgttcc tgagttccat cgcatcaccg gccttcgctc ctatgccggc gacgagtacg
      721 tagagctcaa atggcccgag cactcgcgtt tccccagttg ggggggtgtg atgcgacgga
      781 gcgatctcga ttcccaggtt gcgaccctcg ccgagaagca gggggtcacg atcatggaga
      841 agaccacagc gactcctgtg atcgaggacg gcttcctcac cggtgtcacc ctggaccatg
      901 acgggagtca ggatgtggtc accccgatgg tgtccgtcat cgccgacggc tccatgaatc
      961 gattcgggcg ggagctgggg aacgagcgtc ggcgggacta cccgatgggc atggcggccc
     1021 ggggctacta cgcctcaccc cgctccaagg acccgttcct ggagagccag ctcgatctga
     1081 gagactcaac cggggcgacg atgcccggct acggctgggt gttcccactc ggtgatggca
     1141 cggtcaatgt cggagtgggc cttctctcga ccttcacccg ctggaagcac gtcaacacca
     1201 ctcacatgat ggcggactac gtggccacgg ctcccgacta ttgggagctc tcggaggagt
     1261 ccaagctgac caaccccgtg ggtggcaagt tgacgatgtc tttctcgaaa ggtccgctgg
     1321 tcggggccaa ctggatcacg atcggcgacg ccgccggggc gatcaatcct tggaacggcg
     1381 agggcatttc ctacgcctac gagaccggtc ggatcgccgc cgagcatgtc ggcagggcat
     1441 tgggaactgc agatttcggg ctcttgcgcc gatatccaca acaactcgag gatgaatacg
     1501 ggctctacta caagatggcc cgcatcttcg tgaagctgat cggtcacccg ccggtgatgc
     1561 ggactctggc ccacaccggg ctcaacagcc gatcgttgat ggagtggacg ttgaaggtca
     1621 tggcgaattt gctcgacgaa gacgacaagc acatggggga gcggacatac gatgccctcg
     1681 ccgccgtcgt ccggaccctg ccgacatcgt gaagccggag ctcctcgttt tcgacgtgaa
     1741 cgagacgttg ctcgacatgt cgccattgcg gatcgatttc gaggatgtct ttggcgacgc
     1801 tgatgcgctg ggcgagtggt tcgcccgcct gttgcacggg tccctggtgt cgaaccaact
     1861 cggcgattac cgcgccttcg gcgtgattgg agtagaggcg ctcctgctcg tggccgagcg
     1921 gcgcgggatt gacctcgatg aggcgaccgc caccgagctg gtcacccgtc tcgccatcct
     1981 tccagcccac ctcgacgtca tcccggctct cgagcagctc ctcgatgccg gattccgcac
     2041 cgccgccctc accaacggca gcaccaaggc ggccaacgtc cagatcgaga atgcggggct
     2101 gcataccttc atccaacggg tgatctccgt ggaggaggtg gggaggttca agccggacca
     2161 ggccacctat cgccacgccg cccaggtcat ggatgttcac gtgtccaagt cgatgctcat
     2221 ctcgactcat gactgggatg tggccggggc ggtgggagcc ggcgcctccg ccgccttcgt
     2281 ccggcgaccg gggtacctgt ggactctgcc cgacccgatg cccgaggtgg tgggtggcga
     2341 cctcaaggag atcgccgacc agctgatcgg ttgatcagat ccggggagcg acctcctcgg
     2401 cgagatgtgc catcaactcg acctggctgt cgtaatcaag ggtggggaag tagctccgag
     2461 cggcgaactc gaccgcgatc ccggccttgt cgcgaaggtc gagcaagcta tccacgatct
     2521 cgtcgctcga cccggcgatc gtggcccggc gcagcagggc cccgcggtcg gcttcggtga
     2581 gctctggagc ggccggcggc ggcccggacc tcgtcgccga cgcctccatg tcggagtact
     2641 tccaggtcat ctgccagagg tgctcggtgt accgcgccca ggcgtcgtcg gccgaatcgc
     2701 cgggcaagat caccgagtag tggacgattc gtagctcgga cggatcccgc ccgatgcggt
     2761 cgcactcgtc cctgacccac tcgagctgac ggagaaagcc gttggtcgtg gcattggagt
     2821 agatcccgtc ggagagccgg gcggcacgtc tgatcgccgg ctccgcgccc cctccgatca
     2881 agatcgggac gggttccggt ggagcgggtc tcaccgccac ctcgtcgcac ccgtacaccc
     2941 ggccctcatg ggtgaagggc tggccggtcc aggccttggg gagaatcgtg aggatctcct
     3001 ccatcgctct gccccgctga tcgatccggg cgccgagtcg gtcgaactcg acctcgctcc
     3061 atcccaggcc gagcccgagg gtgaatcgcc caccgctgag caggctgacc gtggccgcgt
     3121 cctcggcgag acggagcggg tgatggagtg gggcgaggat gacaccggtc ccgat
//
LOCUS       DASTXO010000284         5364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_128365, whole genome shotgun sequence.
ACCESSION   DASTXO010000284 DASTXO010000000
VERSION     DASTXO010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128365"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="VFU96_06100"
     CDS             complement(<1..1075)
                     /locus_tag="VFU96_06100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEU5112872.1"
                     /translation="MKVDWVSLTHFRSYEELEWQPDPGVNLLLGPNGAGKTNLLEAIA
                     YLSSLRSFRSVPDSALITDGDDTTVVRAGVSSGPRDRLVEIELSRSGPRRTQLDKTRL
                     QKTADLLGVLRVIAFLPEDLDLVKRGPAYRRDLLDAVAVQLWPAAHPDQAEFDRALRQ
                     RNAFLKSGQRDNTTLSVWDARLAQAGGRVMSRRARVIETLSPHLEAAYGDIAGRPERA
                     AIGYVASWGAGDELDGAAADFASAILASLEKARNVDYDRRLTSVGTHRDEPVLTLDGV
                     DTRTHGSQGEQRTMALAIKLAAHRAVTEMIDEPPVLLLDDVFSELDPQRAASLAKMLP
                     ADTQTLISSARPEDVPVEGEVWRV"
     gene            complement(1076..2158)
                     /gene="dnaN"
                     /locus_tag="VFU96_06105"
     CDS             complement(1076..2158)
                     /gene="dnaN"
                     /locus_tag="VFU96_06105"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU5112873.1"
                     /translation="MRIRAQRDDLADVLARANRAVGARTALPVLQGLLCEVSGTTLRV
                     TGTDLDVTIRTAAEVEVMEEGRAVIPGRLLSEAVRKMPAGAVTIGVSDTDIEIQGNGP
                     RFTLRPLTVDDFPVQEDVVADGVEVDGEELAEAISQVTIAASGDGARPILTGVLFESS
                     DEGLRMVATDSYRLAKRELRGVGLEGTGLVPARGLRELPRSIGSTKVTAQLRDREAVF
                     TSDKGMLRLRLIDGNFPKYQSLLPESYPSQVVLEKDVLLEALGRVTLVAEDHIPVRLK
                     LMEGGVEVAVTRQDVGGETEHLPGVFEGAEEEVSIAFNPRYLQDGVTAISGDSIRIRV
                     IDAFKPSVIDSGAEDDFLYLLMPVRV"
     gene            complement(2331..3719)
                     /gene="dnaA"
                     /locus_tag="VFU96_06110"
     CDS             complement(2331..3719)
                     /gene="dnaA"
                     /locus_tag="VFU96_06110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU5112874.1"
                     /translation="MVQEQMAPGGWGQFQDVLRAGVSKGNWDTWLSRLEPDFAGETPT
                     LVAPSEFHLRWVRDKHGELIESAYRDVFGINTRPVFKVVEPEPGAEQTLPLEEAVVDT
                     AHRVAPIGDGGVPATNLISRYRFESFVVGQSNRFAWAAAMAVAEQPGAHYNPLFIYGG
                     AGLGKTHLLNAVGHQALEMYPNLVVRYVSSENFLNDFIDSIRRKRPDDFKHRYRGVDI
                     LLLDDVQFFEGKEQILEEFFHTFNSLYESGKQMVISSDRHPRNLSSLEDRLRSRFEWG
                     LLTDIQPPDVETRLAILRRNAEFAPRPVPLEVLEHIAVLVSDNIRELEGALTRVTAWS
                     ALNRRDIDLETAERVLRDIVTTEELAPLGPDTIIRATATAYGFTVEDVLSPSRRQPLV
                     LCRQISMYLCRELTDLSLPKIGEHFKRDHTTVLHSVEKVKRILRSDRAVFDRVTQLTQ
                     DLRKGGGISTHA"
     gene            4192..4326
                     /gene="rpmH"
                     /locus_tag="VFU96_06115"
     CDS             4192..4326
                     /gene="rpmH"
                     /locus_tag="VFU96_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU5112875.1"
                     /translation="MKRTYQPKVRRRKRRHGFRHRMQTRAGRGIVKSRRLKGRKRLAA
                     "
     gene            4319..4642
                     /gene="rnpA"
                     /locus_tag="VFU96_06120"
     CDS             4319..4642
                     /gene="rnpA"
                     /locus_tag="VFU96_06120"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEU5112876.1"
                     /translation="MPDFESLRSPRDFQRVLKEGTRRRSGGVVMVKCSGQPGVSRVGL
                     VVSRSAGNAVTRNRIKRRLRHSLRAVQLKPGSDYVFIGSSQVADAPHPQLMGWLERAL
                     EESDV"
     gene            4635..4883
                     /gene="yidD"
                     /locus_tag="VFU96_06125"
     CDS             4635..4883
                     /gene="yidD"
                     /locus_tag="VFU96_06125"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEU5112877.1"
                     /translation="MSDSTFRPRDLVVGLIRIYQKLVSPNLGTNCRYQPTCSQYTAEA
                     IDRFGVIRGSWLGVRRIGRCHPLRPGGYDPVPETWRAH"
     gene            4883..>5364
                     /locus_tag="VFU96_06130"
     CDS             4883..>5364
                     /locus_tag="VFU96_06130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HEU5112878.1"
                     /translation="MGNLFNLLIEGLGSILGFFYDLLDGLPSEGVALGLSIILLTIVV
                     NLLVFPLTLKQTRATRAFTEIQPEIKRIQKEYKDDPQEMQKRLMELQKSAGATPGGCL
                     LPLLVQMPIWFALFRLLQNPSQYIDADSALGLALADGTTKTFLGMDLFTSPAQAMADG
                     "
BASE COUNT          981 a         1699 c         1665 g         1019 t
ORIGIN      
        1 cgacccgcca cacctcgccc tcgaccggca cgtcttcggg gcgtgccgag ctgatcagcg
       61 tctgtgtgtc ggccggcaac atcttggcga gcgaagcggc gcgctgtggg tcgagctcgg
      121 agaagacgtc gtcgagcagc aggacaggag gctcgtcgat catctcggtc accgcccgat
      181 gcgccgcgag cttgatcgca agcgccatcg tccgctgctc accctgactg ccgtgagttc
      241 gggtgtcgac gccgtcgagg gtgagaaccg gctcgtcgcg atgggtgccg acagaggtga
      301 gccgtcggtc gtagtcgacg tttctcgctt tctccagtga cgccaggatg gcggacgcga
      361 aatcggcggc ggcgccgtcg agctcgtcgc ccgcacccca ggaagcgacg tatccgatgg
      421 ccgccctctc cggtctgccc gcgatgtcgc catatgcggc ctccagatgt ggagacaacg
      481 tctcgatcac ccgggcccga cgtgacatga ccctcccacc cgcctgcgcc aggcgggcat
      541 cccaaaccga gagagtcgtg ttgtcgcgct ggcccgattt gagaaaggcg ttcctctgcc
      601 gcaaggcgcg atcgaactcg gcctggtcgg gatgagcggc cggccacagc tgcaccgcga
      661 ccgcgtcgag gaggtcgcgg cgatacgccg gtccccgctt caccaagtcc aggtcctcag
      721 ggaggaacgc gatgacacgc agcacaccga gcaggtcggc ggtcttctgg agacgggttt
      781 tgtccagttg ggtgcgacgt ggcccggacc tggacaactc gatctcgacg aggcggtctc
      841 gcggcccgct ggagaccccg gccctgacca cagtggtgtc gtccccgtcg gtgatgaggg
      901 ccgagtccgg caccgatcgg aacgaccgca gcgatgacag ataggcaatc gcttcgagga
      961 ggttcgtctt gccggcgccg ttgggcccca gaaggagatt gactccgggg tcaggctgcc
     1021 actcgagttc ctcgtaggaa cggaagtgag tgagcgagac ccagtcgacc ttcattcaga
     1081 cccggacggg catcagcagg tagaggaagt cgtcctcggc gccgctgtcg atcacgctcg
     1141 gcttgaacgc gtcgatgacc cggatccgga tcgaatcgcc cgagatcgca gtcaccccgt
     1201 cctggagata cctcgggttg aaggcgatcg acacctcttc ctctgcgcct tcgaacacgc
     1261 cgggaagatg ctcggtctca ccaccgacgt cctgacgagt gaccgccacc tccaccccgc
     1321 cctccatcag cttgagccgc accgggatgt gatcctctgc aaccagggtc acgcgaccca
     1381 gggcttcgag caggacgtcc ttctccagca cgacctggct cggatagctc tccggcaaca
     1441 acgactgata cttggggaag ttcccgtcga tcagccgcag cctcagcatc cccttgtccg
     1501 aggtgaacac tgcctcgcgg tcacgcagtt gagccgtcac cttcgtcgat ccgatcgacc
     1561 gtggcagctc cctcaagcct cgagccggca ccaaacccgt gccctcgaga cccacgcccc
     1621 tgagttcccg cttggcgaga cggtacgagt ccgtggccac catgcgcaga ccctcgtcgg
     1681 agctctcgaa caggaccccg gtcaagatcg ggcgtgcgcc gtcaccggat gccgcaatgg
     1741 tcacctggga aatggcctcg gccagctcct cgccatcgac ctcgacccca tcggcgacca
     1801 cgtcctcttg caccgggaag tcgtcgacgg tcaagggtcg gagggtgaag cgggggccat
     1861 tcccctggat ctcgatgtcc gtgtcactca ccccgatcgt caccgcgccg gcgggcatct
     1921 tccgcaccgc ctccgagagg agccgcccag ggatgaccgc tcttccttcc tccatcacct
     1981 cgacctcggc cgccgtccgg atggtcacat cgaggtcggt gccggtgacc cgtagcgtgg
     2041 taccggacac ctcgcacagc aagccctgca agaccggcaa ggcggtccga gcaccgacgg
     2101 cccggttggc tcgagcgagg acgtcggcga ggtcgtctcg ctgtgctcgg attctcacag
     2161 gcggaggtcc cttcttcttc tgctattgat tttcatatga agagtattag tcgtagtagg
     2221 gggttgtgga tttggggatt aaggggattc tcgcaggttc cgacaggggt ttcgcctgtg
     2281 catcgggatg gggactgtct gtgtgatagt gacctgttct gggggataac tcaggcatgt
     2341 gtggatattc ccccaccttt cctcaggtcc tgtgtgagtt gggtcacccg atcgaatacc
     2401 gcccgatcgg accgcaggat tcgcttcacc ttctccaccg agtggagcac cgtggtgtgg
     2461 tctctcttga aatgctcccc gatcttgggc agagagagat ccgtgagctc ccggcacagg
     2521 tacatcgaga tctgccggca gagaacgaga ggctgccgtc ggctgggact gagcacgtct
     2581 tcgacggtga agccatacgc ggtggcggtg gctcggatga tcgtgtccgg ccccagcggg
     2641 gcgagctcct cggtggtcac gatgtcccgg agcacccgct cggcagtctc gaggtcgata
     2701 tcccgccgat tgagggccga ccatgcggtg actctggtca aggctccttc cagctcacgg
     2761 atgttgtccg agaccaacac cgcgatgtgc tcgaggactt cgagtggaac cggccgcggg
     2821 gcgaactcgg cgtttctcct caggatggcg agacgggttt ccacgtccgg gggctggatg
     2881 tcggtgagga gaccccattc gaaccggctg cgcagccggt cctcgagtga ggagaggttg
     2941 cggggatggc ggtcggagct gatgaccatc tgcttgccgg actcatagag cgagttgaag
     3001 gtgtgaaaga actcctcgag tatctgctcc ttgccctcaa agaattggac gtcgtccaac
     3061 agaaggatgt cgaccccgcg gtagcggtgc ttgaagtcat cggggcgttt gcggcggatc
     3121 gagtcgatga agtcgttgag gaagttctcc gacgagacgt agcggactac cagattgggg
     3181 tacatctcca gggcctgatg tcccaccgcg ttgagcaggt gagtcttgcc caatccggcc
     3241 ccgccgtaga tgaacaacgg gttgtagtga gctccgggct gctcagccac ggccatggcg
     3301 gcggcccacg cgaaccggtt cgactgtccg acgacgaagc tctcgaatcg ataccgggag
     3361 atgaggttgg tcgccgggac acccccgtca ccgatggggg cgactcggtg agcggtgtct
     3421 acgaccgctt cctcgagggg caaggtctgc tcggcacctg gctcgggctc gacgaccttg
     3481 aacaccgggc gggtgttgat cccgaacaca tcgcgatagg cggactcgat caattcgccg
     3541 tgcttgtcac gcacccagcg caaatgaaac tcgctcggtg cgaccagcgt gggagtctca
     3601 ccggcgaaat cgggctcgag acgcgacagc cacgtgtccc agttcccttt cgacactcca
     3661 gcccgcaaca catcctgaaa ttggccccag ccacccggcg ccatctgctc ttgtaccaag
     3721 accactcctc ctgccggaaa ggttgtccac agaaccatcc acatctgtgg aaaggactgt
     3781 ggaacatcca ccgggaacaa tccatcagct cgtcaccgag agcggtccag ccctccgtag
     3841 aggactgagg aagtcgatca cgctcccaag gtcaaggcag gcgagtatcg accctcgatc
     3901 tcgactccgt caagaacgcg agaattgacg atgtggaaga gtcggggtcg ttctatccac
     3961 agggaaatgc caggtgagat atggtttttc cattctcttc cacgggtcgg gagcgctcaa
     4021 acccggtcaa tcgctggtgg cattcttttc gcgagctatc tgctttcttc gcgctccagc
     4081 cccctcgagg tcagaccgaa gcctccgagc ctgtctgacc ggactttgac atagttcggc
     4141 gaggattcgt aacctgtcgt cagtcacccc tctacccacc ctttttttgt catgaagagg
     4201 acttatcaac ccaaggttcg aaggcgaaag cgtcgtcacg gcttccgtca tcgcatgcag
     4261 acccgggccg gacgcggcat cgtcaagtcg cgtcgtctca agggtcggaa gcgtctcgct
     4321 gcctgacttc gaatcccttc gctcccctag agattttcaa agggtgttga aggaggggac
     4381 aagacgccgc tccgggggcg tcgtgatggt gaaatgttcc ggtcagccgg gagtgtcacg
     4441 tgtcgggctc gttgtctcga ggagcgccgg caacgcggtc acccggaata ggatcaagcg
     4501 ccgtcttcga cactctctgc gcgccgtgca gttgaaacca ggatcggatt acgtgttcat
     4561 cggcagcagc caggtagcag acgccccgca tccccaactg atggggtggc tggagcgcgc
     4621 cctggaggag tctgatgtct gattcgacct tccgcccacg tgatctagtg gtcgggttga
     4681 tacggattta ccagaaattg gtaagtccca acctgggcac caattgccgg taccagccga
     4741 cctgctccca atacaccgcg gaggccatcg acaggttcgg ggtgattcgg ggctcctggc
     4801 tcggggtgag acgaatcggt cgctgccatc cgctccgccc cggaggctac gacccggtgc
     4861 ctgagacttg gagagcgcac tgatgggcaa tctgttcaat ctcctcatcg aaggtctggg
     4921 ctcgatcctg ggcttcttct acgacctgct ggatggcttg cccagcgaag gggtcgcact
     4981 cggtctgtcc atcatccttc tcaccatcgt ggtcaacctg ctggtcttcc cgctgactct
     5041 caagcagacc cgggccacca gggcattcac cgagatccaa cccgagatca agcggatcca
     5101 gaaggaatac aaggacgacc cacaggagat gcagaagcgt ctcatggaac tgcagaagtc
     5161 ggcgggggcg acgccgggcg gttgtctcct ccccttgctg gtccagatgc cgatctggtt
     5221 cgccctgttc cgactcctcc agaacccgag ccaatacatc gatgccgact cggcgcttgg
     5281 tctcgcgctg gccgatggga ccacgaagac ctttctcggc atggacctgt tcaccagccc
     5341 ggctcaggcg atggcagacg gatt
//
LOCUS       DASTXO010000285         4172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1233457, whole genome shotgun sequence.
ACCESSION   DASTXO010000285 DASTXO010000000
VERSION     DASTXO010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233457"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VFU96_06135"
     CDS             complement(<1..613)
                     /locus_tag="VFU96_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEU5112879.1"
                     /translation="MIRRILVANRGEIARRVFRTSRRMNMETVAVYSDPDMSEPHVVE
                     ANHAVNLPGSTPSETYLDIDAVLGAAKQTGADAIHPGYGFLAENAEFARRVIDAGLTW
                     IGPSPEAIEVMGSKLRSKDVVEAAGVAILRAIDLSGMAEDAVMEAAAGLGYPVLVKAS
                     AGGGGKGMRVVGDPTDLPEAIEGARREAASAFCDDTVFLEKYLE"
     gene            790..1416
                     /locus_tag="VFU96_06140"
     CDS             790..1416
                     /locus_tag="VFU96_06140"
                     /inference="COORDINATES: protein motif:HMM:NF025380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HEU5112880.1"
                     /translation="MPIAIVSGVVALFIVIGIWVVSALQDLVDAVPTPGDVAAVFEPE
                     PFEEIGPVVISSIRDMSNLTTVETVQYTIVEKGTDRGWLEWARGDSLQLFAVAKIGAG
                     IDMSGISVRNVSLDDQGVVEITVPAAEIQYVSVDSEATQILDRETGLFTKGDPRLETD
                     ARQVAEEVLVQAAIDEGVLTDAEASARQILTDFLLGLGYRDVVVEFAG"
     gene            1418..1825
                     /locus_tag="VFU96_06145"
     CDS             1418..1825
                     /locus_tag="VFU96_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005444240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HEU5112881.1"
                     /translation="MISEEIVVHTGGRAGVFDITSNVARFCSQFEGGLLSVFVPHATA
                     GVAVIEVGAGSDQDLAAAIDDLLPPEPGRWRHEHGSPGHGRDHVLPAFISPSLTIPVI
                     AGHPTLGTWQSVVLVDTNVDNQTRKVRLSFIED"
     gene            complement(1826..3424)
                     /locus_tag="VFU96_06150"
     CDS             complement(1826..3424)
                     /locus_tag="VFU96_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEU5112882.1"
                     /translation="MQVLPTKVDPRSDEYRVNRAAMEEKLAEFEQLMEQARQGGGEKY
                     IKRHRDRGKLLARERIELLLDRDAPFLELSTLAAWGTDKNLGANVVTGIGVVEGVECA
                     ILAHDPTSQAGSMNRYSVAKTLRIMDIAHENRLPLINLVESGGADLPDQAEVFIVGGK
                     WFRNLTRLSKMGIPTIAIVFGSSTAGGAYFPAMCDYAVMVKEQAKVFLGGPPLVKMAT
                     GEESTDEELGGAEMHSKVSGLSDYFAHDEHEAIRMGRDIVSHLNWRKLGYGPTKDPDE
                     PVHDVDDLLGVIPNDLRVPFDMREILARTLDGSRFEEYKAVYGASILTGWGSIHGFPV
                     GVIANQQGVIFSEEAQKATEFIQLTNRYDTPLLFIHNTTGYMVGKDYEQRGIIKDGAK
                     MVNAVANSEVPHIALMAGVSYGAGNYGMSGRSYDPRFVFGYPNHKVAVMGPSQLAGVM
                     SIIARQAAESRGEAFDEEADEKRRKEIEAQIEAEELAYFSTARVRDDGLIDPRDTRHV
                     LGIALSATHSAEVKGTDRFGVFRM"
     gene            3471..3914
                     /locus_tag="VFU96_06155"
     CDS             3471..3914
                     /locus_tag="VFU96_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018103661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEU5112883.1"
                     /translation="MNAADFQDRVGAEIGVSDWMEITQERVDAFAAATDDHQWIHVDR
                     ERAAASPFGGTIAHGFLTLSLTVALSAQVPLDVGSPVMGINYGLEKVRFPAPVPVGSR
                     IRARVGLVSVSDVEGGIQLNRQVTIEVEGTEKPAMVAETVSRLYY"
     gene            complement(3955..>4172)
                     /locus_tag="VFU96_06160"
     CDS             complement(3955..>4172)
                     /locus_tag="VFU96_06160"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5112884.1"
                     /translation="IRVNAVAPGLVSSEMSMAIPEEIRERMVESIPLDRMGTPDEIAN
                     TVWWLLTPLSSYITGEVIVVGGGRSLA"
BASE COUNT          717 a         1326 c         1341 g          788 t
ORIGIN      
        1 cttccaggta cttctccaag aagacggtgt cgtcgcaaaa tgccgacgct gcctcccgtc
       61 gggcaccctc gatcgcctcg ggcaaatccg tgggatcccc cacgactcgc atccccttcc
      121 cgcctccccc cgccgacgcc ttgaccagca ccgggtaccc gagcccagcc gccgcctcca
      181 tgacggcgtc ttcggccatt ccactgagat cgatggcgcg gagaatggcg acaccggcag
      241 cctccaccac atccttcgac cgaagcttcg agcccatgac ctcgattgcc tctggcgagg
      301 ggccgatcca agtcagccct gcgtcgatga cccgccgagc gaattcggcg ttctcggcga
      361 gaaatccgta cccggggtgg atggcatcgg ccccggtctg cttggccgcc cccaggaccg
      421 catcgatgtc caaataggtt tcggaggggg tcgaaccggg aaggtttacc gcatgattcg
      481 cttcgaccac atgcggctcg gacatgtcgg gatccgagta gacggcaacg gtttccatgt
      541 tcattcgccg gctggtgcgg aagacgcggc gagcgatctc accccggttg gcgacgagga
      601 ttcttcggat catcggctcg cgcagcgtac cggtggaaac catttccgac gtcaggacgt
      661 cataactagg tcgttcaaga acctgagttg aggagtcgcg cacaagtgag tgatacaaag
      721 ttgatggaga ggcaggatcg agagaccgtc gagaggctcg agcctgcccc gaggcggagg
      781 tcaccgtggg tgccaattgc gatcgtctcc ggcgtggtgg ctctgttcat cgtgatcgga
      841 atctgggtgg tgtcggctct ccaggatctg gtcgacgccg tgccaacccc aggagacgtg
      901 gcggccgtgt tcgagccgga gcccttcgag gagatcggcc cggtggtcat cagctcgatc
      961 cgtgacatgt ccaatctgac cacggtcgag accgtccagt acacgatcgt cgagaagggc
     1021 accgaccggg gatggctgga atgggcacgg ggtgacagcc tccagctgtt tgccgtggcc
     1081 aagatcggtg cgggcatcga catgagtggg atctcggtgc gcaatgtctc gttggacgat
     1141 cagggtgtgg tggagatcac cgtccccgcg gcggagatcc agtacgtgtc ggtggacagc
     1201 gaggcaaccc agatcctcga ccgggagaca ggcctgttca ccaagggtga ccccaggttg
     1261 gagaccgacg cccgccaggt cgccgaggag gtcctggtgc aggccgccat cgacgagggg
     1321 gtgctcaccg atgccgaggc cagtgctcgt cagatcctga ccgacttctt gctcgggctc
     1381 ggctaccgcg acgtcgtggt cgaattcgcc ggctgaggtg atcagcgaag agatcgtcgt
     1441 ccacacgggt ggccgagccg gtgtcttcga tatcacatca aacgtcgcca gattctgctc
     1501 tcagttcgag ggaggcctgc tgtcggtgtt cgtccctcat gccaccgccg gtgtggccgt
     1561 gatcgaggtg ggggcaggga gtgatcagga cctcgccgcg gccatcgacg acctgctccc
     1621 gccggaacct gggcgatggc gccacgagca cggcagtccc ggtcatggtc gcgatcacgt
     1681 gctgccggcg ttcatttctc ccagcctcac cattccggtg atcgccggcc atccgacatt
     1741 gggcacctgg cagtcggtgg tcctggtaga caccaacgtc gacaaccaga cgcggaaggt
     1801 ccgtctttcc ttcatcgagg actgatcaca tccgaaacac gccgaagcga tcggtcccct
     1861 tcacttctgc ggagtgtgtc gcggagagcg cgatgccgag aacgtgtctc gtgtcgcggg
     1921 ggtcgatcaa cccatcgtcc cggacccggg cagtggagaa gtacgccagc tcctcggctt
     1981 cgatctgagc ctcgatctcc ttacgacgct tctcgtccgc ctcctcatcg aacgcttctc
     2041 cccgtgattc cgccgcttgc cgggcgatga tcgacatgac cccggccaat tgggaaggcc
     2101 ccatcaccgc caccttgtgg ttgggatagc cgaagacgaa ccgtgggtcg tacgaccgtc
     2161 cggacatgcc gtaattccct gctccgtaag agaccccggc catgagtgcg atgtggggca
     2221 cctcggagtt ggctacggca ttgaccatct tcgccccgtc cttgatgatt cccctctgct
     2281 cgtagtcctt gcccaccatg tatccggtgg tgttgtggat gaagagaaga ggcgtgtcgt
     2341 accggttggt gagttggatg aactcggtgg ccttctgcgc ttcttcagag aagatcaccc
     2401 cttgctgatt ggcgatcaca ccgaccggga agccgtggat agatccccac ccggtgagga
     2461 tggatgcgcc atagaccgcc ttgtactcct cgaagcggga gccgtcgaga gtccgggcga
     2521 ggatctccct catgtcgaag gggaccctga ggtcgttcgg gatcacgccg aggaggtcgt
     2581 cgacatcgtg caccggctcg tccgggtcct tggtcggccc gtagcccagc ttgcgccagt
     2641 tgaggtggct gacgatgtca cggcccatcc ggatggcctc gtgctcgtca tgggcgaagt
     2701 agtcggagag acccgagacc ttcgagtgca tctcggcgcc accgagctcc tcgtcggtcg
     2761 actcttcccc ggtcgccatc ttcaccagcg gcgggccgcc gaggaacacc tttgcctgct
     2821 ccttcaccat gacggcgtag tcgcacattg cagggaagta ggcgcctcct gccgtcgagc
     2881 tgccaaacac tatggcaatc gtggggatgc ccatcttgga gaggcgggtg aggttgcgga
     2941 accacttgcc tccgacgatg aacacctcgg cttggtcggg aaggtcggca ccgcccgact
     3001 cgaccaggtt gatgaggggg agtcgattct catgggcgat gtccatgatc cgaagggttt
     3061 tcgccaccga gtatcgattc atcgagccgg cctgactggt cggatcgtgg gcgaggatcg
     3121 cgcattcgac gccctcgacg actccaatcc cggtcaccac gttggcaccg aggttcttgt
     3181 cggtgcccca ggcggcgagg gtggagagct cgaggaatgg ggcgtcgcga tcgagcagca
     3241 gttcgatccg ctcccgggcc aggagcttgc cacggtcgcg atggcgcttg atgtacttct
     3301 cgcccccgcc ctgccgggcc tgctccatga gctgctcgaa ctcggccagc ttctcctcca
     3361 tggcggcccg gttgacccga tactcatccg accggggatc gaccttggtg gggagaacct
     3421 gcacattcct aatgtacggc gtcacacgag cctgacgggg tgcagaacgg atgaatgcgg
     3481 cggacttcca ggaccgggtc ggcgccgaga tcggggtgag cgattggatg gagatcaccc
     3541 aagagcgggt cgacgccttc gccgccgcca ccgatgacca ccagtggatc cacgtcgacc
     3601 gtgaacgggc ggcggccagc ccgttcgggg gcaccatcgc ccatggcttc ttgaccttgt
     3661 ccctgaccgt ggccctgtcc gcccaagtgc ccctcgatgt cggctccccc gtcatgggca
     3721 tcaactatgg gttggagaag gtccgctttc ctgctccggt ccccgtcggc tcccggatcc
     3781 gggcccgcgt cggcctggtc tcggtcagcg acgtcgaggg tgggatccag ctcaaccgtc
     3841 aggtcaccat cgaggtcgaa ggcaccgaga agccggccat ggtcgccgag acggtgagcc
     3901 ggctgtacta ctgaccgcgg gcaaaggtca ccagcggtgg cccgtcacca ggcatcaggc
     3961 aaggctcctc cctccgccta ccacgatgac ctcgccggtg atgtagctgg agaggggggt
     4021 gaggagccac cagacggtgt ttgcgatttc gtccggcgtc cccatccggt cgagggggat
     4081 ggactcgacc attctctccc ggatctcctc ggggatggcc atgctcatct ctgaggagac
     4141 gaggcccggg gccaccgcgt tgaccctgat gt
//
LOCUS       DASTXO010000286         7964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1822903, whole genome shotgun sequence.
ACCESSION   DASTXO010000286 DASTXO010000000
VERSION     DASTXO010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7964
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1822903"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VFU96_06165"
     CDS             <1..465
                     /locus_tag="VFU96_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112885.1"
                     /translation="QSGAIAIAIDSYEPVLDEVYRGSAGTGVAVALTDLANPSPSQGW
                     AGQERASLMERARPDLVIAYGVIHHLIYGASIPPREVVRWLSTFRCPVVLEFVSPSDE
                     MVSKLVGNKLPHELHPGGEESEFRAILDQSFVTIDEHVLSSETRVLFALEPR"
     gene            462..2555
                     /locus_tag="VFU96_06170"
     CDS             462..2555
                     /locus_tag="VFU96_06170"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU5112886.1"
                     /translation="MRPAQLGSTPWLTVPLSLAAVGAFALAQPVLDLLGRNPEFFIAR
                     GFTAIDVVIFPALLLLLPALLSLPVLALRWIGPRTAGIGHAVVIGALFSLFVANAWVA
                     LFGSDTSPLLFGLAVAGAGVAFACTFLAFPVVQTGTVYAAWAVPIFAAWFLFMTPSGD
                     VALARSGDLPEVGEVQNPVPIVLLVFDEFPVATMIDSRGNLLSELFPNFAELAEDGIW
                     YRNAVGVRQQTEEALPTILSGVGADEGSVPVFSDHPLNLFTLFSDTYDIAAVETVTNL
                     CPDFACSNSSRRIDPWPKRWAGLGVDIAVVYGHLTTPRAVSDRLPAIDQTWGNFTTGE
                     RSSFDIIDRFLANVDDDRRLEVDRLLDTFEFDDAEPPLRFGHFLYPHHPWEVTADGHR
                     TGANSSPGSEGAGWGNDPWLVGQGYQRHILQSQYADTIVGSVIDRLREEGVYDQALVM
                     VVADHGITIGPGVANQRLITEDTIGTIAAVPMFVKYPSGQGGVEPGTTDDVRAETVDI
                     VPTIAEVTRTVIPWAMDGTSLLDPARDQRTQSVMVGREGPVTFGVVGDEKLAAAAVKE
                     TWFPDGNPWLLTPAGWQQWLGRPVAEATATDDEEVTVTVRQQDLLDEVPPDPDLLPVF
                     LSGTLSLGREANGEEIVVAAVDGVVRAVTRTFEPEGSSARFQVMIPPDLLHPGENDVV
                     LWVADGDPSDPAFVR"
     gene            2600..2923
                     /locus_tag="VFU96_06175"
     CDS             2600..2923
                     /locus_tag="VFU96_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112887.1"
                     /translation="MGLMDTIKGWFGTAKEQGADIADQATEKGSELIDNAEPYVEKAK
                     DFAQDAADRADTKLDELGHSGSEAVDSAQEKAAQAADAARDTAGKATDAVEGAIDEAK
                     DQLGG"
     gene            2969..4144
                     /locus_tag="VFU96_06180"
     CDS             2969..4144
                     /locus_tag="VFU96_06180"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018505146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU5112888.1"
                     /translation="MKRASYRLSNVPPSATLGISARARELRAEGHDVISFGAGEPDFA
                     TPEHVVAAAARACYDPENHKYTASAGLRPLREAIAEDVGRYSGVEVDWSEVMVTNGAK
                     QAVFQAFAALLDPGDEVLLPSPHWVTYPAGIDLAGGVTVSVPTRIEDEFKVDVDGLER
                     ARTDRTRLLVFVSPSNPTGAVYTEDEARAIGEWAKTNDIWVMADEIYQRLTYFTEVAP
                     SIAAVTPRFDNWVLINGVAKSYSMTGWRVGWMVGPADIIDAANSLQSHATGNVDNVAQ
                     HAALAALRGPQDTVGEMRQAFDNRRKLMFSLVSDIPGIRCVEPQGAFYVFPDVSGALD
                     GRWASTDELATVILEEAGVALVPGESFGTPGYLRLSYATGEAEIETGMERIARLLAG"
     gene            4181..5254
                     /locus_tag="VFU96_06185"
     CDS             4181..5254
                     /locus_tag="VFU96_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium chain dehydrogenase/reductase family
                     protein"
                     /protein_id="HEU5112889.1"
                     /translation="MEGTTARAMMLVEPRHFSLTELPARPGLDGGWLAVEATGISGAD
                     VQVWKGEAKDVVYPLIPGHEVVGRIARGGPSIPFPVGTRVVVEPRIHCGECHRCKLNL
                     ATCVRRRPSNSYGFIPSIEPPGLWGGLAEFLYIDPAARLHPLTDDVPAEVGTFVHPLA
                     AGHTWAVELPNLQNGENLLILGPGPRGLACLIAAKAAGAGWVGITGLSHDRDRLELAK
                     RLGADLAIDAEKEDVGGAIAEAIGRRPDIVVDVTSDDPEALFTALDLVRSGGRVILAS
                     TKGNRAFHFISDVLVAKQLTIRGAMGASTVGYQWASRQIETDPRIDDLVSHQFPLSES
                     SRALQAAAGLLGREELISVAVTF"
     gene            5256..6536
                     /locus_tag="VFU96_06190"
     CDS             5256..6536
                     /locus_tag="VFU96_06190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5112890.1"
                     /translation="MTGRGVAPGRLSVWSWVIYDLANTIFALGVIGRYFPEWLISVDQ
                     PDSALAVVEAGAAALVIFLAPWAGARSDVRGTRVPTLILTTIGCVAATALLASGPVSV
                     TLALLGLALIAFNVGSVVYDALLVDVSTPANRGRISGLGVGVGYIGSFVGLGIGVVTL
                     DVLGWSYAATFLVLALAFLVLALPAFFFIDERPGHDDAPLPAPRDIARRLAGSWRLAG
                     TYDGVVPFLVGRFLYTDAINTLIGGFLTIFVIQELGLDGQFTTRLLGLAIASAIVGAF
                     VAGGLVERLGPIRVLRAVLIVWMVAIGAGIAAAVTGMTGLAWAIGPLGGLALGATWTA
                     DRVVMTRVSPPRHLGEFYGLYATVGRFATILGPLTWALIVDVLGLSRRVAMGALIGFI
                     VIGWWVLRQVDDSERDWPTADLPVVSTAPDAPLK"
     gene            complement(6505..7467)
                     /locus_tag="VFU96_06195"
     CDS             complement(6505..7467)
                     /locus_tag="VFU96_06195"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEU5112891.1"
                     /translation="MTDSGARFSLVSGEGDGAMLGLAAGDTAGGAWELGYSAVTEQAT
                     VIAYQLIEVRALDLDAVARALRELDGSNEEEPVYRAESPHFRAWLDRAAAGRPVPDED
                     PSLDAAPRAVPLGVTHRRDPGRLRDEALALGRLFHDHPASILGGVIAAGAVAASCFGQ
                     SGRDLVTGIVEITEPAASTLAPDPASGAEPGDLVDGLRQLARYVGVADGDEALALITD
                     GRDPLPWDLVRAGLLLSAPVVDRSHLPVEQAARIGGSPLAAMVGGIVGARVGIRAWPW
                     AFANDTWFAEIGRRLVRGPDEVRDLPIPYAVEHHLISGERQGLY"
     gene            7503..>7964
                     /locus_tag="VFU96_06200"
     CDS             7503..>7964
                     /locus_tag="VFU96_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylglycerol kinase family protein"
                     /protein_id="HEU5112892.1"
                     /translation="MDRIVVVANPSASQFTGGAHRTVMSVLGRVADVETVWPETAADA
                     ETKTAQAVEGGARVVVAMGGDGIVHHVAQGLVETDVALGIIPVGTTNVIARLLGVPAK
                     PAKAARLIVDSDAPILTMGTARMTLSRGSVETTHHGVFACGFGLDAVVVLEA"
BASE COUNT         1306 a         2654 c         2613 g         1391 t
ORIGIN      
        1 caaagcggcg ccatcgcgat cgccatcgac agctatgagc cggtgctcga cgaggtctat
       61 cggggaagtg ccggcaccgg tgtcgccgtc gccctcaccg acctggccaa cccgtcgccc
      121 tcccagggct gggcgggtca ggagagagcc tcgctgatgg agagagcccg ccccgatctg
      181 gtgatcgcct atggcgtcat ccaccacctg atctacggag ccagcatccc tccccgcgag
      241 gtcgtgcgat ggctctccac attccgatgc cccgtcgtcc tcgaattcgt ctcaccatca
      301 gacgagatgg tgagcaagct ggtcggcaac aagctccccc acgagctcca tcccggaggc
      361 gaggagtcgg agttccgggc gattctcgac cagtcgttcg tcaccatcga cgaacatgtt
      421 ctttcctcgg agacgagggt ccttttcgca ctcgagccgc gatgaggccg gcgcagctgg
      481 gttcgacgcc ctggttgacg gtgccgttgt cgctggcggc ggtgggcgct tttgccctgg
      541 ctcagcctgt gctggacctc ctggggagaa acccggagtt cttcatcgcg cgtggattca
      601 ccgcgatcga cgtcgtcatc ttccctgcgc tcctcctcct cctgcctgcg ctgctctcgc
      661 tcccggtgct cgcccttcgt tggatcgggc cgcgtaccgc gggtattggt cacgccgtgg
      721 tcatcggagc gttgttctcg ctcttcgtgg ccaacgcgtg ggtagccctg ttcgggagtg
      781 atacctcccc gctcctgttc ggtttggccg ttgccggggc gggagtggct ttcgcctgca
      841 cgttcctcgc cttcccggtg gttcagaccg ggactgtcta cgcagcatgg gccgtgccca
      901 tcttcgccgc atggttcctc ttcatgaccc cgtccggcga cgtggcattg gcccgctccg
      961 gcgacttgcc cgaagtcggc gaggtccaga acccggtgcc catcgtgttg ttggttttcg
     1021 acgagttccc ggtagcgacg atgatcgact cccggggcaa cctcctcagc gaactcttcc
     1081 ccaatttcgc cgagcttgcc gaagatggca tctggtaccg gaatgcggtg ggggtccggc
     1141 aacagaccga agaggccctt cccaccatcc tgagcggggt cggggcggac gaaggaagcg
     1201 tcccggtctt ctcggaccat cccctgaacc tcttcaccct gttctcggac acctatgaca
     1261 tcgccgcggt ggagacggtc accaatctct gcccggactt cgcatgtagc aactccagca
     1321 ggcggatcga cccctggccg aagcgatggg cggggctcgg cgtcgacatc gccgtggtct
     1381 acgggcatct cacgacgccg cgggcagtgt cggaccgcct gcccgcgata gatcagacgt
     1441 ggggcaactt caccaccggt gagcgctcga gcttcgacat catcgatcgg ttcctggcca
     1501 acgtcgatga cgaccgacga ctcgaggttg accgactcct cgacactttc gaattcgatg
     1561 acgccgagcc accgctgcgc ttcggccatt tcctataccc ccaccaccct tgggaggtca
     1621 ccgccgacgg gcaccgcacg ggcgccaaca gctcccccgg gtcagaggga gcaggctggg
     1681 gcaacgaccc ctggctcgtg ggccaggggt accagcgaca cattctccag tcgcaatatg
     1741 ccgacaccat cgtcggctcg gtgatcgacc ggctgcgtga ggagggtgtc tacgaccagg
     1801 cactggtcat ggtggtggcc gatcacggga tcaccatcgg ccccggagtg gccaatcaac
     1861 gattgatcac agaggacacg atcggcacca tcgccgcggt ccccatgttc gtcaagtacc
     1921 cgagcgggca gggaggcgtg gaaccgggga cgaccgacga tgtgagggcc gagaccgtcg
     1981 acatcgtccc caccatcgcc gaggtcaccc gaaccgtcat cccgtgggcg atggatggca
     2041 cgtcgctgct cgacccagcg cgggatcagc gaacccagtc ggtgatggtg gggagggagg
     2101 ggccggtcac cttcggagtc gttggcgacg agaagctcgc ggccgctgcg gtaaaggaga
     2161 cctggttccc tgatggaaac ccttggctgc tcaccccggc cggctggcag caatggctcg
     2221 gacgcccggt ggccgaagca accgccaccg acgacgagga ggtcacggtg acggtccggc
     2281 aacaggacct gctggatgag gtgccccctg acccggacct ccttccggtt ttcctctccg
     2341 ggaccttgag tctcggtcgg gaggcgaatg gggaagagat cgtggtggcg gcggtggacg
     2401 gagtggtcag ggcggtgacc cggacattcg agccggaggg ctcttcggct cgtttccagg
     2461 tgatgatccc cccagatctc cttcaccccg gggagaacga cgtggtgctc tgggtagccg
     2521 atggcgatcc atccgatcct gcgttcgtca ggtgacgctt gcccatcacc tacgattggc
     2581 ctctcgagaa gggaacgaca tgggattgat ggacaccatc aaaggatggt tcgggacagc
     2641 caaggagcag ggagccgata tcgccgacca ggcaacggag aaaggctccg agctgatcga
     2701 caacgccgag ccctacgtcg agaaggccaa ggactttgcc caggacgccg cggacagggc
     2761 cgacaccaag ctcgacgagc tcgggcactc gggctctgag gcagtcgaca gcgcccagga
     2821 gaaggcggca caagccgctg atgcagcccg ggacaccgcc ggcaaggcga cagacgcagt
     2881 agaaggcgcc atcgatgagg caaaggacca gttgggcggc tgatcgaagc tcgatgacga
     2941 tccccgatcc ctcgacgacg aaatgacgat gaaacgcgcc tcctaccgac tcagcaacgt
     3001 tcccccatcc gcgactctcg gcataagcgc acgggccagg gaactgcgcg ccgagggcca
     3061 cgacgtgatc agtttcggcg ccggtgagcc cgacttcgcc acacccgagc atgtcgtggc
     3121 cgccgcggcg cgtgcctgct acgacccgga gaaccacaaa tacacggctt cagccggctt
     3181 gcggccgctg cgtgaggcca tcgccgaaga cgtcggccgc tattcgggag tggaggtcga
     3241 ctggtccgag gtgatggtca ccaacggggc caagcaggcc gtgttccagg cattcgccgc
     3301 ccttctcgat cctggcgacg aggtgctgct cccctcgccg cattgggtca cctaccccgc
     3361 aggcatcgac ctcgccggtg gcgtcaccgt ctcggtaccg acgaggatcg aggacgagtt
     3421 caaggtcgat gtggacggcc tcgagagggc gcgcactgac cgcacccggc tcctggtatt
     3481 cgtctcaccc tcgaacccaa ccggagcggt ctacaccgag gacgaggctc gcgccatcgg
     3541 agagtgggcc aagacgaacg acatctgggt gatggccgat gagatctacc agcgcctcac
     3601 ctacttcacc gaagtggctc cgtcgatagc ggcggtgacc cctcggttcg acaattgggt
     3661 cctgatcaac ggggtggcca agtcctactc catgactgga tggcgggtgg gttggatggt
     3721 gggcccggcc gacatcatcg atgccgccaa cagtctccag tcccacgcca ccggcaacgt
     3781 cgacaacgtt gcccagcacg cggccctggc cgcgctgcgc ggccctcagg acactgtcgg
     3841 tgagatgcgc caggcgttcg acaacagacg caagctcatg ttctcgctgg tcagcgacat
     3901 accgggtatc agatgcgtcg agccccaggg tgccttctat gtgttcccgg acgtgtcagg
     3961 tgcattggac ggccggtggg cttccaccga cgagctcgcc acggtcatcc tcgaggaggc
     4021 aggggtcgcc ctggtccctg gcgagtcgtt cggcacaccc ggatacctgc gactctccta
     4081 cgccacgggg gaggccgaga tcgaaacggg aatggagcgc atagcccgtc tcctggccgg
     4141 atgactggcg ggttctgaac cgggtttaca ctcgccatcg atggaaggca cgactgcgcg
     4201 ggcgatgatg ctcgttgagc cccgtcactt ctcgctcacc gagttgccgg ccagacccgg
     4261 tctcgacggc ggctggctcg cagtggaagc gaccgggatc tcgggggcag acgtccaggt
     4321 gtggaaggga gaggcgaagg atgtcgtcta cccgctgatc ccagggcacg aagtggtggg
     4381 aaggatcgcc cggggcggcc catcgatacc cttcccggtt gggacccggg tcgtggtgga
     4441 gccgaggatc cactgcggag agtgtcatcg ctgcaagctc aacctggcga cctgtgtccg
     4501 ccgccgaccc tccaactcct atggtttcat cccttcgatc gagcctcccg gactttgggg
     4561 tggtctcgcc gagttcctct atatcgaccc ggcggcacgg cttcacccac tcactgacga
     4621 cgtccccgcg gaagtcggga cctttgtcca cccgctggcg gcagggcaca cctgggcggt
     4681 cgagttgccc aatctccaga atggagaaaa tttgctcatc ctgggccccg gtcctcgcgg
     4741 gctggcctgc ttgatcgcgg ccaaggcggc cggagcaggg tgggtgggca taaccggctt
     4801 gtcccacgat cgggaccgcc tcgagctcgc caagcggctc ggcgccgacc tcgccatcga
     4861 tgccgagaaa gaagacgtcg ggggcgccat cgccgaagcg atcgggcgca gaccggacat
     4921 cgtcgtcgac gtcacctcgg acgacccgga agcgcttttc accgccctcg acctggttcg
     4981 cagcggcggt cgtgtcatcc tcgcctcgac caagggcaac cgggcctttc atttcatcag
     5041 cgacgtcctc gttgccaagc agctcacgat caggggcgcc atgggggctt ccaccgtcgg
     5101 ataccagtgg gcatcccgcc agatcgaaac cgatccacga atcgacgatc tcgtctcgca
     5161 tcagttcccg ctctccgagt catctcgcgc ccttcaggcg gccgccgggt tgctcgggcg
     5221 tgaggagctg atctcagtgg ccgtaacgtt ctgacatgac cggtcgcggg gtggccccgg
     5281 gccggttgtc cgtctggtcc tgggtcatct acgacctggc caacacgatc ttcgccctcg
     5341 gagtcattgg gcgctatttc cccgagtggc tgatctccgt cgaccagccg gatagtgccc
     5401 tcgccgtggt cgaggccggt gccgcggctc tcgtgatatt cctggccccg tgggccggag
     5461 cacgcagcga tgtccggggc accagggtgc cgactctgat cctcacgacg attggctgcg
     5521 tggcagccac ggcactgctc gcctcgggcc cggtgagtgt gaccctggcc cttctcggcc
     5581 tggccctcat cgcgttcaac gtgggatcag tcgtgtacga cgccctcctc gtcgatgtca
     5641 gcaccccggc caaccggggt cgcatatcag ggcttggggt cggagtcgga tacatcggat
     5701 cgttcgtagg tctcggcatt ggcgtcgtga cactcgatgt cctcggctgg tcgtacgccg
     5761 ccacgttcct ggtgctggcc ctcgccttcc tggtactcgc cctgccggcc tttttcttca
     5821 tcgacgaacg acccggtcac gacgacgcgc cactgccggc gcccagggat atcgcgagaa
     5881 ggctggccgg ctcatggcgt ttggcaggca cctacgacgg ggtggtcccg ttcctcgtgg
     5941 gccggttcct ctacaccgat gcgatcaaca cgctcatcgg cggcttcctc accatcttcg
     6001 tcatccagga gctgggtctc gacgggcagt tcacgaccag gctgctcgga ttggccatcg
     6061 catccgccat cgttggtgcc ttcgtcgcgg gaggactggt ggagagactc gggccgattc
     6121 gggtgttgcg cgccgtgctc atcgtgtgga tggtggcgat cggcgcgggg atcgccgccg
     6181 cggtgacggg catgaccggc ctggcctggg ccatcgggcc cttgggtggg ctcgccctcg
     6241 gggcaacctg gaccgcggat cgggtggtga tgacccgggt gtcgccacca cgtcacctgg
     6301 gtgagttcta cgggctgtat gcgaccgtgg gccggttcgc caccatcctc ggcccgttga
     6361 cctgggcatt gatcgtggat gtgttggggc tcagccggcg cgttgccatg ggcgccctca
     6421 tcggcttcat cgtcatcggc tggtgggtgc tgcgacaggt cgacgattcg gaacgggact
     6481 ggcccaccgc cgatctcccg gtcgtcagta cagcccctga cgctcccctg aaatgaggtg
     6541 gtgctccacg gcgtagggaa tcggcaggtc ccggacctcg tcaggcccgc ggaccaatct
     6601 cctcccgatc tcggcgaacc aggtgtcgtt ggcgaatgcc cagggccagg cccggatacc
     6661 gacccgggcg cccacgattc caccgaccat agcggcaagc gggctcccgc cgatacgggc
     6721 agcctgctcc accgggagat gcgaccggtc cactaccggg gcggagagga gcagacccgc
     6781 cctgaccagg tcccagggca acggatcacg gccatcggtg atgagagcga gtgcctcgtc
     6841 gccgtcggcc acacccacat agcgggcgag ctggcgcaac ccatcgacga gatcgcctgg
     6901 ttcggcgccg gaggctggat ccggtgccag ggtggacgcg gccggctcgg tgatctccac
     6961 gatccccgtg acgagatctc gaccggactg tccgaagcaa gaagctgcga cggcgccggc
     7021 ggcgatgacg ccaccgagga tcgatgccgg gtggtcgtga aagagcctcc cgagagcgag
     7081 cgcctcatcg cgaagccggc ccgggtcgcg gcggtgcgtc accccgagtg ggaccgccct
     7141 cggcgccgcg tcgagagatg ggtcctcatc gggaaccggg cgcccggcgg ccgctcggtc
     7201 gagccaggcg cggaaatgag ggctctcggc tcgatacacc ggttcttcct cgttcgatcc
     7261 gtccagctca cgcagggcgc gagcaacggc atcgagatcc agagcgcgga cctcgatgag
     7321 ttgataggcg ataacggtcg cttgctctgt gaccgcgctg taacccagct cccaggcccc
     7381 gccggcggta tcgccggcgg caaggcccaa catcgctcca tccccctcgc ctgacacgag
     7441 cgagaacctc gctcccgagt ctgtcacggt gcgggagggt acatctgtag gctcgacgcc
     7501 aaatggaccg catcgtggtc gtcgccaatc cctcggcatc ccagttcacc ggtggcgccc
     7561 atcggaccgt gatgtcggta ttgggacgag tcgcggatgt cgagacggtg tggcccgaga
     7621 cggcggccga cgccgagacc aagaccgcac aggccgtcga gggcggcgcc cgcgtcgtcg
     7681 ttgcgatggg tggggacggc atcgtccacc acgtggcaca gggcctggtc gagaccgatg
     7741 tcgcgctcgg catcatcccg gtcggcacca cgaacgtcat cgcccggctg ctcggagtcc
     7801 ccgccaagcc ggcgaaagcc gcccgtctga tcgtcgacag tgacgccccg atcctgacga
     7861 tgggcacggc gcggatgacc ctctcccgtg gctcggtcga gacgacacat catggcgtct
     7921 ttgcctgtgg gttcggcctg gatgccgtgg tcgtcctcga ggcc
//
LOCUS       DASTXO010000287         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1822908, whole genome shotgun sequence.
ACCESSION   DASTXO010000287 DASTXO010000000
VERSION     DASTXO010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1822908"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1216
                     /locus_tag="VFU96_06205"
     CDS             104..1216
                     /locus_tag="VFU96_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112893.1"
                     /translation="MSSFTKRGSADLEATLRRMVVVFRFLGLIWMLILVAVTVLNDPP
                     PVMWIVWAAVGLAVFWTFLTWLVAKTQPEFMAGWWWFALDAVAMLAIGAASVASGAEE
                     LFHGGLPLSLVFTGALLGGLPGSLIAALLLGVEQFLVHVIADLGAVRAAGSVIFFVVG
                     AIVGWTFDKLRDYDLARQAAQDELAAEQAAVVLHEERAALADRLHDSVLQTLHAIRMG
                     ADDPGQSRYLARRQERELRRNIEEWRSEFRDSFRANLLAVRDEVEDTHRVEIEAVIRD
                     DAPIDPNLSAAIEAAREAMANAAKHSGSPNITLYSEFANGEAHIHVRDSGTGFADDGQ
                     RNRVHERLARRVENVGGLVEIDTAESGTEVKITVQK"
     gene            1213..1863
                     /locus_tag="VFU96_06210"
     CDS             1213..1863
                     /locus_tag="VFU96_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5112894.1"
                     /translation="MTDRLRIIIVDDHDVVRAGMRAILAPSFDIVGEADNVDSAIELI
                     KERLPDLVVLDVKLPGGGGAAVIPAVRRDFPDIRFMALTVSTSKEDVARLLDAGVDGY
                     ITKTTLGADLPDLVRQTYVGARPVSPDVAAYLLDIDEDITGETPISRLTPREREVVNL
                     IARGYTYRESAERLGITVKTLENHMAHIFDKLSVASRHELTALAYEEGYVRPDDER"
BASE COUNT          381 a          681 c          677 g          363 t
ORIGIN      
        1 ggaccgcgga agcggtcccc ctttgaacgc gaaaacggac gccatccaac tctcacctct
       61 cccaaagacc aacccgccac ccaccccgct agtttcgggt gaaatgagca gcttcaccaa
      121 gcgaggcagc gccgatctcg aggcaaccct ccgacggatg gttgtggtct tccgtttcct
      181 cggtctcatc tggatgctga tcctggttgc ggtgaccgtc ttgaatgacc cgccccccgt
      241 catgtggatt gtgtgggcgg cagtgggcct cgccgtcttc tggaccttcc tgacctggct
      301 cgtcgccaag acccagcccg agttcatggc cggctggtgg tggtttgccc tggacgcggt
      361 ggccatgctc gccatcggag cggcatccgt cgcgtcggga gccgaggagc ttttccacgg
      421 tggcctgccc ctgtctctcg tcttcaccgg ggctctgctg ggtggcctcc ccgggtcgct
      481 gatcgcggcg cttctgctcg gagtcgagca gtttctcgtc cacgtgatcg ccgatcttgg
      541 agcggtgcgg gctgcgggca gcgtgatctt cttcgtggtc ggcgcaatcg tcggttggac
      601 cttcgacaaa cttcgtgact atgacctcgc ccggcaggcg gctcaggacg agcttgccgc
      661 cgagcaggcc gcggtggtgc tccacgagga gcgggctgcg ctcgcagacc gtctccacga
      721 ctcggtgctg cagacccttc acgccatccg aatgggggcg gacgacccgg gccagtcccg
      781 atatctggcc agacgtcagg aacgcgagct acggcgaaac atcgaggagt ggcgctccga
      841 gttcagggac agcttccggg cgaatctgct cgcggtgaga gatgaggtgg aggacaccca
      901 cagggtcgag atcgaggcgg tgatccggga cgacgccccg atcgacccca acctgtcggc
      961 cgccatcgag gccgcccgtg aggctatggc caacgcggcg aaacattcgg gctcacccaa
     1021 catcacgctg tattccgagt tcgccaacgg tgaggcccac atccatgtcc gggacagcgg
     1081 gaccggcttc gcagacgacg gccagcgaaa tcgggtgcac gagcgtcttg cccgccgggt
     1141 cgagaacgtc ggtggcctgg tggagatcga caccgccgaa tcgggcactg aggtcaagat
     1201 cacggtccag aaatgaccga tcgccttcgc atcatcatcg tcgatgatca cgatgtcgtc
     1261 cgcgccggaa tgcgggcaat cctcgctcct tcgttcgaca tagtcggtga agccgacaac
     1321 gtggacagcg cgatcgagtt gatcaaggag cggctcccgg acctggttgt cctcgacgtc
     1381 aaactgcccg gcggtggagg cgcggccgtg atccccgcag tgcgcaggga cttccccgat
     1441 atccgcttca tggcccttac cgtctcgacg tcgaaggagg atgtggcgag gctcctcgac
     1501 gccggcgtcg atggctacat cacgaagacg acgctggggg ctgatcttcc ggatctcgtc
     1561 cgccagacat acgtcggggc ccggccggta tcaccagacg tggccgccta tctcctcgac
     1621 atcgatgagg acatcaccgg ggagaccccc atctcgcgcc tgactccgcg ggagcgggaa
     1681 gtggtgaacc tcatcgcccg cgggtacacc tatcgggaga gcgccgagcg gctcgggata
     1741 accgtgaaga ccctggagaa ccacatggcg cacatcttcg acaagttgag cgtcgccagc
     1801 cgtcacgagc tcaccgccct cgcctatgag gagggctacg tccggcccga cgacgagagg
     1861 tgacacggat agggggaatc ccccattccc gcttggtgaa agcggatgta gaaatacggt
     1921 gggtgggcaa gggaccttcc tcccggagcc acatcccgga ggggggagga caagagaacg
     1981 gcttctctga agccagcaag cccccgtcgt aagacggggg cttttgcatc agaactggac
     2041 cgcggccgcg gcccaatgaa agccggctcc gaaagccgtc aggacgacca gatcgccatc
     2101 cc
//
LOCUS       DASTXO010000288         2304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1307495, whole genome shotgun sequence.
ACCESSION   DASTXO010000288 DASTXO010000000
VERSION     DASTXO010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307495"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VFU96_06215"
     CDS             complement(<1..309)
                     /locus_tag="VFU96_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112895.1"
                     /translation="MSQEPEVLVAAGTAEDGVRGQQKIYEIASSGSGRVRGRTREVTI
                     SEPELNAFLSRHLAEAAKTPFASGTVRLVGDGFIEFKGQLPLRRVVVIADLLPARWLE
                     R"
     gene            736..1044
                     /locus_tag="VFU96_06220"
     CDS             736..1044
                     /locus_tag="VFU96_06220"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEU5112896.1"
                     /translation="MHETKDDLVLNFELPGVTDKDVSLSITGDLLTVKGERAFNRDSH
                     AENTHHIERVYGKFERSVQLPMPVQADKVKATYRDGVLEVRLPKVEAVKPKAIKIDIA
                     "
     gene            1134..>2304
                     /gene="dnaK"
                     /locus_tag="VFU96_06225"
     CDS             1134..>2304
                     /gene="dnaK"
                     /locus_tag="VFU96_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEU5112897.1"
                     /translation="MAKVIGIDLGTTNSVVAVVEGGNPTVIANQEGSRLTPSVAGFTK
                     DGEILVGQVAKRQAITNPENTVFSIKRFMGRRHDEVLQEIKLVPYKVVKAANGDARIE
                     VRGKQYSPPEISAMILRKLKEAAEAYLGEKVTQAVITVPAYFNDSQRQATKDAGRVAG
                     LEVLRIINEPTAAALAYGLDKKKDETIAVYDLGGGTFDISILEIGEGVFEVKATNGDT
                     HLGGDDFDQRVMDWIAGEFKKEHGIDLLKDRMALQRLKEAAEKAKCELSTTLQTEINL
                     PFITADASGPKHLVLTLTRAKLEALVADLVERTLGPCRQAMQDAGVSPKDINEVILVG
                     GQIRMPKVQEAVKQLFEKEPHKGVNPDEVVAVGAAVQGAVLTGEVKDLLLLDVTPL"
BASE COUNT          505 a          693 c          722 g          384 t
ORIGIN      
        1 ccgctcgagc caccgagcag gcaggaggtc agcaattacc acgacgcgac ggagcggcaa
       61 ctggcccttg aattcgatga agccatcccc gacgaggcgg acagttcccg acgcgaaggg
      121 agtcttagcg gcttcagcca agtgacggga gagaaacgcg ttcagttctg gttcggagat
      181 ggtgacttca cgagtgcgcc cccggacccg acccgaccca ctactcgcga tctcatagat
      241 cttctgctgc ccacgaacac cgtcttcggc cgttcctgcg gcaaccagga cctctggctc
      301 ttgggacatt cgaaggccca cccagacgga gccagcagcc agcccggcta cgcctaggag
      361 gcctatgaca atgatcgtac atcccagtcg catcgcccca ccctatcacc cgagccttca
      421 aaaacccttt gatattacgt cgatgctgcc caatctcaag ctggaccgtc gcccatcttg
      481 acatgatcat actcagatga tatataacta tcgtactcat aacaggccgg gcgagggaga
      541 ccggtcagag ccaataggag ggcttgcgtc atggcgattg aacgttggcg gccgttcgta
      601 tccgttgaga ggtgggatcc attccggaac atgtccgata tccagggaga ggtcaatcgc
      661 ctcttcgaca gtttcctcgg aaggccgatg gcctcgacgg gatcgaccgt acgaagctgg
      721 gccccggttc tcgacatgca tgagaccaag gacgatctcg tcctgaattt cgagcttccg
      781 ggcgtcaccg acaaggacgt gtccttgtcg atcaccggag acctgctaac agtgaagggt
      841 gagcgcgcat tcaaccgcga ctcgcacgcc gagaatacgc accacatcga gcgtgtctac
      901 ggcaagtttg agcgtagcgt gcaactcccg atgcccgtgc aggcggacaa ggtcaaggcg
      961 acctaccgcg atggcgtgct cgaggtcagg ctcccgaagg tggaggcagt gaagcccaag
     1021 gcgatcaaga tcgacatcgc ctaggtcggt tgcaccgcac gaacgtgtga ttcgacggag
     1081 cgggaacgac ccgctccgtc gccgtgagac caaggagaaa gggagattca accatggcga
     1141 aggtgatcgg cattgacctc gggacgacca actccgtggt agcggtcgtc gagggcggca
     1201 acccgacggt gatcgcgaac caggagggca gccgactcac gccctcggtc gcgggattca
     1261 cgaaggacgg cgagattctc gtgggccagg tcgccaaacg gcaggcgatc acgaatccgg
     1321 agaacaccgt gttctcgatc aagcggttca tggggcgtcg ccacgacgag gtgctccagg
     1381 aaatcaagct cgtcccgtac aaggtggtca aggccgccaa cggcgatgcc cggatcgagg
     1441 tgagaggtaa gcaatactca ccgcccgaga tctcggccat gattctgcgc aagctgaagg
     1501 aggccgccga ggcgtatctg ggtgagaaag tgacccaggc cgtcatcacc gtgcccgcct
     1561 acttcaacga cagccagcgg caggcaacca aggatgccgg ccgggtggca ggtctcgaag
     1621 tgctgcggat catcaacgag cctaccgccg cagccctggc gtacggcctc gacaagaaga
     1681 aggacgagac gatcgccgtg tacgacctcg gtggcggcac cttcgacatc tccattcttg
     1741 agattggcga gggtgtattc gaggtcaagg cgaccaacgg tgacacccac ctgggcggcg
     1801 acgacttcga ccagcgcgtc atggactgga tcgcggggga attcaagaag gagcacggca
     1861 tcgacctcct caaggaccgc atggcgcttc agcgtttgaa ggaggccgcc gagaaggcga
     1921 agtgtgagct gtccacgacg ctgcagaccg agatcaatct gcccttcatc accgcggatg
     1981 ccagcgggcc gaagcatctc gtcctcacgc tgacgagggc caagctcgaa gcgctcgtgg
     2041 cggaccttgt cgagcgcact ctcggcccat gccggcaggc catgcaggac gccggtgtga
     2101 gcccgaagga tatcaatgag gtcatcttgg tgggcggcca gatccgcatg ccgaaggtgc
     2161 aggaagccgt caagcagctc ttcgagaagg agccgcacaa gggcgtcaac ccggacgaag
     2221 tcgttgcggt cggtgcggcc gtgcagggcg cggtgctcac gggtgaggtg aaggatctgc
     2281 ttctgctcga tgtcactccg ctct
//
LOCUS       DASTXO010000289         2625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1197076, whole genome shotgun sequence.
ACCESSION   DASTXO010000289 DASTXO010000000
VERSION     DASTXO010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197076"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1207)
                     /locus_tag="VFU96_06230"
     CDS             complement(11..1207)
                     /locus_tag="VFU96_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5112898.1"
                     /translation="MKELAPDSSAEPGRSIWALDPSVRHLNHGSFGGVLNEVLEAQTE
                     WRRRFEADPSRFVFRELQPAMDRTREAVGDFVGAEAGGLVPVRNATTGIASVLRSFEP
                     RLRPGDQLLTTAHDYNAIRQILRFTAERTGAEVVIAPVPFPIELPGQVTDAILGAVSP
                     KTRLAVIDHITSPTALVFPVEDIVAALEPEIPVVVDGAHGPGQLPLALDDLGASWYTG
                     NLHKWVCAPKGAAFLHTRADWIEITVPAVISHGYNAEVESPQARYRALFDWLGTDDFS
                     AWAVVPDALRLVGGLDAGGWDGLMKRNHDLVLEGRRLIAETIALSLPAPDQMVGSMAA
                     VVLPDARGPDPGGDLSPMMDDLFDAGLSAIVMNWPSWPRQLLRISAHLHNTLEDYAAL
                     AARLRS"
     gene            1265..>2625
                     /locus_tag="VFU96_06235"
     CDS             1265..>2625
                     /locus_tag="VFU96_06235"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEU5112899.1"
                     /translation="MTKLDFGFSQVTEDKVSTGIDLAIEQAQQILEDLVAPKETRTFE
                     NTMLPLDQIADVLSKANARYAFMGYVHPEKEVRSAAKAAEEKLETFGVEMVFRDDLNA
                     AITEYAATDEAEGLEGERARLVEFTLRDLRHAGHELSPEARARVKERTQRLVELGVRF
                     QENIDEWEDHILVDPEELDGLPQSFADSLETDEETGKLKVTLAYPHLIPFVENATRRD
                     LREVLSFKFNTQAVEANRKILEEARDLRHQIAEEFGVPSWAHHRLEERMAKDPERVAA
                     FYEDLVPPLTKQGEIDLATMGGLLDSDVGDSQIQTWDWRYYDTQQRRTDYGVDPFEVA
                     NYFPLDLVLGGMFDLVQETFGLEFREVPDPDVWHPDVRLFAIHDAASGDELAHFYLDL
                     FPREGKYGHAAEFPLIMSRRLEDGSYQNPVCAMVANFTKPTATAPSLLQHGEVETLFH
                     EFG"
BASE COUNT          520 a          837 c          838 g          430 t
ORIGIN      
        1 caaggtcgac tcaggaacgc aatcgcgcgg ccaatgccgc atagtcctcc aacgtgttgt
       61 ggagatgagc cgagatgcgg agcaactgcc ggggccagct cggccagttc atgacgatgg
      121 ccgataaccc ggcatcgaac agatcatcca tcatcgggga cagatcgcca cccggatcgg
      181 ggccgcgggc gtcggggagc accacggctg ccatggagcc caccatctga tccggcgccg
      241 gcagtgacag ggcgatggtc tccgcgatga gacgccggcc ctccaggacg aggtcgtggt
      301 tgcgtttcat caaaccgtcc catccccctg cgtccagacc ccccactagg cgcagcgcgt
      361 cggggactac cgcccaggca ctgaaatcgt cagtgcccag ccagtcaaag agcgcccggt
      421 aacgggcctg ggggctctcg acctcggcgt tgtatccatg ggagatcacg gctggaaccg
      481 tgatctcgat ccaatcggcc cgagtgtgga ggaaggccgc cccttttggc gcacagaccc
      541 acttgtgcag gttgccggtg taccaggagg cacccagatc atccagtgcc agtggcaact
      601 gcccagggcc gtgcgccccg tcgacgacca cagggatctc cggctcgagg gcagccacga
      661 tgtcttccac cgggaacacg agcgcggtcg gcgaggtgat gtggtcgatg accgccagcc
      721 tcgtcttcgg cgacaccgct ccgaggatcg catctgtgac ctgcccgggg agctcgatcg
      781 ggaagggaac cggcgcaatg acgacttcgg ccccggtccg ctcggccgtg aaacgcagga
      841 tctgacgaat ggcgttgtag tcgtgggcgg tcgtcagcag ctgatccccg gggcggagcc
      901 gtggctcgaa cgagcgcaga accgaggcga tcccggtagt cgcgttgcga accggcacca
      961 gaccgccggc ctcggcgccg acgaaatcac cgacagcctc ccgcgtgcgg tccattgccg
     1021 gctgcaattc ccggaacacg aaacggctcg gatcggcctc gaatcgccgc cgccactcgg
     1081 tctgtgcctc gagcacctcg ttcaacaccc cgccgaatga cccatggttc agatgacgca
     1141 cagagggatc gagtgcccag atcgaccggc ccggctcggc actcgaatct ggggcgagtt
     1201 ctttcatagc tgaaacgtag aagctgtgat tggctgccgc ggcctcctcg ctagcgtgcc
     1261 gtgcatgacg aagctggatt ttgggttctc ccaggtgacc gaggacaagg tgagcacagg
     1321 gatcgacctg gcgatagaac aggcccagca gatactcgag gacctcgtgg cgcccaagga
     1381 gacccggacc ttcgagaaca ccatgctccc cctcgaccag atcgcagacg tgctgtccaa
     1441 ggccaacgcc aggtacgcat tcatgggcta tgtgcatccg gagaaggagg tccgctcggc
     1501 ggcgaaagcg gccgaagaga agctggagac ctttggcgtc gagatggtgt tccgtgacga
     1561 cctcaatgcc gcaatcactg agtacgccgc caccgacgag gcagaaggcc tggagggcga
     1621 gcgggcacgc ttggtcgagt tcacgctcag agacctacgt catgcgggtc acgagctgtc
     1681 ccccgaagcc cgggccagag tcaaggagag gacacagcgc ctggtcgagc tcggggtccg
     1741 cttccaggag aacatcgacg aatgggagga ccacatcctc gtcgaccccg aggagctcga
     1801 cggcttgcca cagagctttg cggactcatt ggagaccgac gaggagaccg gcaagctgaa
     1861 ggtgaccctc gcctacccac atctgatccc cttcgtcgag aacgcgacgc gtcgtgatct
     1921 gcgcgaggtg ctgtccttca agttcaacac acaggcggtc gaggccaatc gaaagatcct
     1981 cgaggaggcg cgcgaccttc gccaccagat cgcggaggag ttcggcgtgc cgagttgggc
     2041 ccatcaccgt ctcgaggaga ggatggcgaa ggatccggaa cgggtggcgg ctttctatga
     2101 ggatctcgtg cccccactca ccaagcaggg ggagatcgat ctggcaacga tgggtggtct
     2161 gctcgacagc gacgtgggtg acagtcagat ccaaacctgg gactggcgct actacgacac
     2221 ccagcagcga cgcaccgact acggcgtgga cccattcgag gtggccaact acttcccgct
     2281 cgatttggtg ctcgggggca tgttcgatct agtgcaggag acatttggcc tcgagttccg
     2341 agaggttccc gatccggatg tctggcatcc cgatgtgcgt ttgttcgcca tccacgacgc
     2401 cgcaagcggc gacgagctcg ctcacttcta tctcgatctc ttcccccgcg agggcaagta
     2461 cgggcacgcc gccgagttcc ctctgatcat gagcaggcga ttggaggacg ggtcgtatca
     2521 gaacccggtg tgcgccatgg tcgccaactt caccaagccg acggcgacag ctccttcctt
     2581 gctccagcac ggcgaggtcg agacactctt ccacgagttc ggcca
//
LOCUS       DASTXO010000290         3246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1123385, whole genome shotgun sequence.
ACCESSION   DASTXO010000290 DASTXO010000000
VERSION     DASTXO010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123385"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VFU96_06240"
     CDS             <1..324
                     /locus_tag="VFU96_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009466640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEU5112900.1"
                     /translation="GTLGGMRETLAKRQNWVDQGKQVAKATVVKAYGSAPRGPGSLML
                     VSSAGEMEGSVSGGCVENDVVVRAINVLETGEPELVTYGIADDDAFAVGLACGGTIQV
                     YIEPW"
     gene            321..1076
                     /locus_tag="VFU96_06245"
     CDS             321..1076
                     /locus_tag="VFU96_06245"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEU5112901.1"
                     /translation="MTSPVLEATQTLVEKDAFGVVISVIAGPEAGSKAVLDDTGGILA
                     GSLPDRVTESAQADALALMSRETSTTLAYGEDELFFEVIAPRPRLFVFGAVHIAQELV
                     QHAALLGFHTVVADPRPAFVTKERFPDADELRVGWPGDVVDAGSFDARTFVVVLTHDR
                     RFEDPLWPLLLPTDVRYIGAMGSSKTSAARRQRLLDSGFTAEQVDRIHGPIGLDIGSR
                     AAGEVAIAILGEMISARYLHDRELSLQGRPVRV"
     gene            1076..1678
                     /locus_tag="VFU96_06250"
     CDS             1076..1678
                     /locus_tag="VFU96_06250"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU5112902.1"
                     /translation="MTTAAVVLAAGASRRLGRSKQLEPWGDSNLLSNVVARTRAFPVD
                     EVWVVLGHDYERILEETDFGDAGVIENPEWAEGIASSIRVALDALTRLSRVDKALIVM
                     GDQPSVPVDLVDQLIDSHARSDHPVSVPKYRYSWGNPVLVDRALWPRLMSLEGDEGAQ
                     RLWQAHPEWVNEVWFSDLAPRDVDTETDIVELRPKHPGST"
     gene            1731..2129
                     /locus_tag="VFU96_06255"
     CDS             1731..2129
                     /locus_tag="VFU96_06255"
                     /inference="COORDINATES: protein motif:HMM:NF025589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HEU5112903.1"
                     /translation="MARIDQVAGIAHKDATKLRKAGVRTSKALIEVAAERKGRADLSA
                     TTGIDARHLQLWVNHADLLRVKGVGAEYAELLTAAGVDTLRDLRRRNPTALLAKIIGL
                     NGSNRVVQRLPTESMVEVWIEAAKELDPSS"
     gene            complement(2188..2673)
                     /locus_tag="VFU96_06260"
     CDS             complement(2188..2673)
                     /locus_tag="VFU96_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112904.1"
                     /translation="MKTKNVLYAVVGAPVVAARKVGSKAGDFRSKLSEGATEYSQVAE
                     KAIDSWAVEGEKVVGRITDGKVVEEISSKVDLDQAKEQVSKLRDQLEDMLATWRTSFR
                     PEKASSKDSAKETSAKAPAKKPATKAATTPKASAAKTTTSSAAKTSPAKTNSASAEKA
                     S"
     gene            complement(2676..3044)
                     /locus_tag="VFU96_06265"
     CDS             complement(2676..3044)
                     /locus_tag="VFU96_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5112905.1"
                     /translation="MNESKQNDLGEFIHQQRERANLSLRRLAERAGISNPYLSQIERG
                     IRRPSAEILSRLSRALEISANTLYSRAGLIDEDLDSPTIYEAVDADSLLSANEKKLLL
                     DMYRALIHSNGSVDKQSEQE"
BASE COUNT          544 a         1018 c         1048 g          636 t
ORIGIN      
        1 ggtactcttg gaggcatgcg tgagacgctg gcaaagcggc agaattgggt ggaccagggg
       61 aagcaggtgg caaaggccac cgtggttaag gcgtacgggt cggcgcccag aggcccgggc
      121 tcgctgatgc tggtctcctc cgcgggggag atggaagggt cggtgtcggg gggctgtgtc
      181 gagaacgacg tcgttgtccg tgcgatcaac gtcctcgaaa ccggcgagcc agagctggtc
      241 acctacggca tcgccgacga cgacgccttc gcggtgggtc tcgcctgcgg cgggaccatc
      301 caggtctaca tcgaaccgtg gtgacatcac cggtactcga agccacccaa accctggtcg
      361 agaaggatgc cttcggcgtc gtgatctcgg tcatcgccgg cccggaagct gggtccaagg
      421 cggtgctgga cgacaccggc gggatccttg ccggatcact tcccgaccgg gtgacggaat
      481 cggcgcaggc ggatgctctg gccctgatgt ctcgagagac gagcacaact ctcgcctacg
      541 gcgaggacga gttgttcttc gaggtgatcg ccccccgtcc acgtttgttc gtgttcgggg
      601 cagtgcacat cgcccaggag ttggtgcagc atgctgccct gctcggcttt cacacggtgg
      661 tggccgaccc gcggcccgcg ttcgtgacga aggagcgctt ccccgacgcc gatgagctgc
      721 gggtgggatg gcccggcgac gtagtcgacg cgggctcatt cgatgcccgc accttcgtcg
      781 tggtgctgac ccatgaccgg cggttcgagg acccgctgtg gccccttctt ctcccgaccg
      841 atgtgaggta tatcggggcg atggggtctt cgaagacttc tgccgcccga cgccagcgcc
      901 tgctcgactc gggtttcacg gccgagcagg tcgaccgcat tcacgggccg atcggtctcg
      961 atatcggttc ccgggctgcg ggtgaggtcg cgatagccat cctgggggag atgatctccg
     1021 cccgctacct gcacgatcga gagctgagtc tccagggccg gccggtgcgg gtctgatgac
     1081 caccgcggcc gtcgtcctcg ccgctggggc gtcccgccgc ttgggccgtt ccaagcagct
     1141 cgagccctgg ggtgactcca atctcctctc caacgtcgta gcgaggaccc gggctttccc
     1201 cgtggacgaa gtctgggtcg tgctcggtca cgactacgag cggatcctcg aagagaccga
     1261 cttcggagat gccggtgtga tcgagaaccc ggaatgggcc gagggcatcg cctcatccat
     1321 ccgggttgcg ctcgacgcct tgacgagact ctcccgcgtc gacaaggccc tgatcgtcat
     1381 gggtgatcag ccttcggtcc cggtggacct cgtcgaccag ctgatcgact cgcatgctcg
     1441 ctcagaccat ccggtgagtg ttcccaagta ccgctactcc tggggcaacc cggtgctggt
     1501 cgaccgtgcc ctgtggccgc gcctcatgtc tctagagggc gatgagggag cgcagcgtct
     1561 ctggcaggcg caccccgaat gggtgaacga agtttggttc tccgacctcg ctccgcgcga
     1621 tgtggacacc gagaccgaca tcgtcgaatt acggcccaag caccccggtt ccacctgatt
     1681 tcatctgtct agagtcgcgt cgtctggttt cttttgcgct gaagggacac atggctcgga
     1741 tcgatcaggt agcggggatc gcccacaagg acgccaccaa gctgcgtaag gcaggggttc
     1801 ggaccagcaa ggcactcatc gaagtcgcgg cggagcgaaa ggggagagcc gatctctctg
     1861 ctaccaccgg catcgatgcg aggcatcttc aactctgggt gaatcatgct gaccttctcc
     1921 gtgtcaaggg tgttggcgcc gagtacgccg agctcctgac cgccgccggc gtcgacacct
     1981 tgcgtgatct gaggcgccga aacccaaccg ccctcctggc caagatcatc gggctgaacg
     2041 gcagcaatcg cgtggtgcaa cggctcccca ccgaatcgat ggtcgaggtc tggatcgagg
     2101 cggccaagga actcgatccc agcagctgag aagcgcatga agaaggggcc ccgaagggcc
     2161 ccttcttctc tttgaggagg agtgagctca ggaagccttc tcggctgacg ccgagttggt
     2221 cttcgcaggg ctcgtcttcg ctgcggacga cgtggtcgtc ttggcagcag aagccttggg
     2281 ggtagtggca gccttggtgg ccggcttctt ggcaggtgcc ttggccgagg tctccttggc
     2341 tgagtccttg ctggacgcct tctcgggtcg gaaggatgtc ctccaggtgg ccagcatgtc
     2401 ctcgagctgg tcgcgcagct tgctcacctg ctctttggcc tgatcgaggt cgaccttcga
     2461 ggagatctcc tcgacgacct ttccgtcggt gatccggccg acgaccttct caccttcgac
     2521 ggcccagctg tcgatcgcct tctcggcgac ctggctgtac tcggtggcac cctcgctcag
     2581 cttcgagcga aagtcccctg ccttggagcc gaccttgcgg gcggcgacca ccggggcacc
     2641 tacgacggca tacagcacgt tcttcgtttt catcgtcact cctgttcgga ttgtttgtct
     2701 actgacccat tggaatggat caacgctcga tacatgtcga gaagcagctt cttctcattg
     2761 gcgctcaaca ggctgtctgc gtcgaccgcc tcgtagatcg tgggcgagtc caagtcctcg
     2821 tcgatgagac ccgctcggga atagagcgtg ttcgccgaga tctcgagggc ccgcgacaga
     2881 cggctgagga tctcagctga aggccggcgg ataccacgct cgatctggga cagatagggg
     2941 ttggagatcc cggcccgctc cgccagacga cggagcgaga ggttggctct ttccctctgc
     3001 tgatggatga actcgccgag gtcgttctgc ttgctctcgt tcactttgcg caagcttaca
     3061 gcaagctgct aactattgca agcatgtgct agcggagagc ggactcagac cagggcggtg
     3121 ccgaccgcac tcccgatcag ccaggcgatc accccgccga tcccggcgat ggccatgttc
     3181 tcgagacccg agatcagcca gctgtgcttc gccaccctcg ccttgatcac accaaccagg
     3241 aagagg
//
LOCUS       DASTXO010000291         3486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1639040, whole genome shotgun sequence.
ACCESSION   DASTXO010000291 DASTXO010000000
VERSION     DASTXO010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1639040"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /gene="dctP"
                     /locus_tag="VFU96_06270"
     CDS             <1..519
                     /gene="dctP"
                     /locus_tag="VFU96_06270"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEU5112906.1"
                     /translation="LRPIRTPEDCREMTVRLQPNAIHEELIRSWGAIPVAVELSAGIE
                     LISRLEVDAQENPLANTVAYGVDRVHRHVTMTGHLYGARGLFSHRPTWEGFAPDLRDV
                     VSNAARSAIDVQRRAARAMESTLRAKLEDDGVEFVDLTDDERSVFVEGSSPAISLAHR
                     DLPEDLFELARS"
     gene            516..1262
                     /locus_tag="VFU96_06275"
     CDS             516..1262
                     /locus_tag="VFU96_06275"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5112907.1"
                     /translation="MTHDFEDRVVVVTGAAVGIGKATAAAFGDAGATVYLVDVDADAG
                     PASAEAVTGATFIHCDLTSEEEVTATVEAIDRASGRVDVLVNNAGGFPETLGLEETSL
                     GEWHRILDVNLTSVFLMTRATLPLLRRSDGGRIVNLGSLAGQTAGWQTAPPYVAAKAG
                     VHGLTRALATELAREGITVNAVAPSAVLTERIARLRDESQLAATAASIPLGRYQSPEE
                     VAEWVMFLASPAAGFMTGQTVSVNGGRFMA"
     gene            1279..1662
                     /locus_tag="VFU96_06280"
     CDS             1279..1662
                     /locus_tag="VFU96_06280"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5112908.1"
                     /translation="MARLIRFDEADPVARGDGIESVRLTPTPLEGQGFTVGITSFPPG
                     TSIRLHSHNTIEQVTVLEGEGLAELNGEQVPARPYDTTQIAPGEWHRFVNTGGGTMRI
                     LWVYGSTEVTRTFADTGETVDQFER"
     gene            1659..2696
                     /locus_tag="VFU96_06285"
     CDS             1659..2696
                     /locus_tag="VFU96_06285"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEU5112909.1"
                     /translation="MRFAFERLVAFSRDVLVALDVPPDHAEVTARRLLEADLRGRTGH
                     GLIRIPQYVSRIEAGGINLRPEITVPRETPVSALVDGDNGLGQVVMTLAAETAMAKAA
                     ESGMAWVGTVHSNHAGAAGVYTNMAVRRNLIAMYIAVASANVMPPWGGRDRLLGTNPI
                     SIAIPAGSSDPFVLDIATTVTSHGTIKVLAQAGEQMPEGWVVDMEGRPILDPNRADEG
                     YLMPIGGYKGAGLNIALGLLAGVLNGAAFGADVIDHRKVPGRAANTGQAMFVMRPDLF
                     VDLDDFRAGIDHHLDSLRGAGAPGEVHLPGEVAAALEDEQLVEGIPVGDVLLGQLRDL
                     GARLGLDDPLE"
     gene            complement(2703..>3486)
                     /locus_tag="VFU96_06290"
     CDS             complement(2703..>3486)
                     /locus_tag="VFU96_06290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEU5112910.1"
                     /translation="LAAAQAAAHRGTSSFCALLTGSLWEEVAATAPGRHFFQIYPMGD
                     RTWMTDVADRAEQAGFAGICVTVDSPVIGRRDRSLESGFSWAVPAEGTNSLSRHGFDY
                     AYRATFTWPDLAWLCAHTALPVILKGVMTGDDAIAAVECGVAAVYVSNHGGRMVDQGV
                     STIEVLAEVIEAVGPRAEVAIDSGFTRGAEVCKALALGARAIGIGRLQCWGLALGGAG
                     GLARVLEILEQEIGSTMANLGSSSIADMTPDLVRWSIAAAPE"
BASE COUNT          605 a         1147 c         1144 g          590 t
ORIGIN      
        1 ctccgtccca tccgcactcc cgaagactgt cgcgagatga cggtgcggct gcagccaaac
       61 gccattcatg aggagttgat tcgctcgtgg ggagcgatcc cggtggcggt agagctcagc
      121 gcgggcatcg agttgatctc gcgtctcgag gtcgacgccc aggagaaccc gctggccaac
      181 acggtggcct atggggtcga tcgagtccac cgtcacgtga ccatgaccgg gcacctctac
      241 ggggcgagag ggttgttctc ccatcggcca acgtgggaag gtttcgcccc cgatctgcgg
      301 gacgtggtgt ccaacgccgc tagatctgcc atcgacgtcc agcgacgcgc cgccagggcc
      361 atggagtcga cgcttcgggc aaaattggag gatgacgggg tcgagttcgt ggatctgacc
      421 gacgacgagc gttcggtctt cgtggagggc tcttcgccag ccatctccct ggcccatcga
      481 gatcttccgg aggacctctt cgagctggct cggtcatgac gcacgacttc gaagaccggg
      541 tcgtagtcgt caccggcgcc gcagtgggca tcggaaaagc cactgctgcc gccttcgggg
      601 acgccggggc cactgtctac ctcgtcgatg tggatgccga cgccggaccg gcctcggccg
      661 aggcagtcac cggcgccaca ttcatccatt gcgacctgac ttctgaggag gaggtgacgg
      721 ccaccgtcga ggccatcgac cgggcctcgg ggagagtcga cgtcctcgtg aacaatgcag
      781 gcgggttccc ggagaccctc ggcctggagg agacctcgct gggtgaatgg catcgaattc
      841 tcgacgtcaa cctgacttcg gtgtttttga tgactcgggc cacgcttccc ttgctgcgcc
      901 gatcggatgg cgggcggatc gtcaacctcg ggtcgctggc gggtcagaca gcgggctggc
      961 agacggcgcc gccgtacgtc gccgccaagg ccggggtcca tggcctgacc cgggccctcg
     1021 ccaccgagct cgccagagaa gggatcaccg tgaatgccgt cgccccctcc gcagtcctca
     1081 ccgagcgcat cgccagactt cgcgacgaat cccaactcgc tgcgaccgcg gcatccatcc
     1141 ccttaggtcg ctatcagtcc cccgaagagg tcgccgaatg ggtgatgttc ctggcctcgc
     1201 ccgccgccgg gttcatgacc ggccagaccg tatcggtgaa cggcggtagg ttcatggcat
     1261 gagaccaggg gagggatcat ggcccggttg atccgattcg acgaggccga cccggtggcg
     1321 cgtggggatg ggatcgagag cgtccgcctc accccaacgc ccctcgaggg acaggggttc
     1381 acggtgggca tcacgagctt tccaccagga accagcatcc gcctccacag tcacaacacc
     1441 atcgaacagg tgacggtgct cgagggcgaa ggtctcgccg agttgaacgg tgagcaagtc
     1501 ccggcccgtc cctacgacac gacacagatt gcccccgggg agtggcaccg gttcgtcaac
     1561 accgggggcg ggacgatgag gatcctctgg gtgtatggga gcaccgaggt cacccggacc
     1621 tttgccgaca caggggagac cgtggaccaa ttcgagcggt gagattcgcc ttcgagcgtc
     1681 tcgtcgcatt ctcccgagac gtcctcgttg ccctcgatgt gccgcccgat cacgcggagg
     1741 tcaccgcccg ccgcctgctc gaggctgatc tacgggggag gacgggtcac ggcttgatcc
     1801 ggatcccgca atacgtatcg cggatcgagg cggggggcat caatctccgc cctgagatca
     1861 ccgtccctcg cgagacaccg gtgagcgcac tcgtggacgg tgacaacggc ctcggccagg
     1921 tggtgatgac cctggccgcc gagaccgcga tggccaaggc ggctgagtcg ggcatggcgt
     1981 gggtgggcac tgtccattcc aatcatgccg gcgcggccgg ggtgtacacg aacatggctg
     2041 ttcgaaggaa cctcatcgcc atgtacatcg ccgtggccag tgccaacgtg atgccgccct
     2101 ggggcggtcg agaccgattg ctggggacca atccgatctc gatcgccatc ccagcaggct
     2161 cgagcgaccc gtttgttctc gacatcgcca ccacggtgac atcgcacggc acgatcaagg
     2221 tgctggctca ggccggggag cagatgcccg agggctgggt ggtggacatg gagggacggc
     2281 ccatcctcga cccgaaccgc gccgatgagg gctacctgat gccgattggg ggctacaagg
     2341 gggcgggcct caacatcgcc ctcggactgc tcgccggtgt cctcaacggc gccgcattcg
     2401 gggccgacgt gatcgatcac cgcaaagtgc cgggtcgagc ggccaacacc gggcaggcga
     2461 tgttcgtgat gcgccccgac ctcttcgtgg atctcgacga ctttcgcgcc gggatcgatc
     2521 accatctcga ttcgctccgg ggtgccgggg cgccgggtga ggtccatctc cccggtgagg
     2581 tggccgccgc gttggaggac gaacagttgg tggaggggat cccggtcggt gacgtgcttc
     2641 tcggccaact ccgtgacctc ggagcccgat tggggttgga cgatccactc gagtgatctg
     2701 gatcattccg gtgccgcggc gatcgaccaa cgaaccaggt cgggggtcat gtcggcgatc
     2761 gagctggatc ccagattggc catcgtgctg ccgatttcct gctccaggat ctcgagcaca
     2821 cgcgccagcc cgccggcgcc accgagtgcc agcccccagc actgaagacg cccgatccca
     2881 attgctcgtg ctcccagggc gagcgccttg cacacctctg ccccgcgggt gaacccactg
     2941 tcgatggcca cttcggcgcg aggcccgacc gcctcgatga cctcggccag cacttcgatc
     3001 gtgctcacac cctgatcgac catgcgacca ccgtgattgg agacgtacac ggccgctacc
     3061 ccgcattcga cggctgcgat cgcgtcatct ccggtcatga ctcccttgag gatcacgggc
     3121 aatgcggtgt gggcacagag ccaggcgagg tcaggccagg tgaaagtcgc ccggtaggcg
     3181 taatcgaatc cgtgtcgact caggctgttc gtcccttccg ccggcactgc ccaagaaaac
     3241 ccactttcca gactccggtc acgacgcccg atcaccggag agtcgacggt cacacatatg
     3301 cccgcgaacc cggcttgctc cgccctgtcg gcgacatcgg tcatccaggt tcgatctccc
     3361 atcggataga tctggaagaa atgtcggccg ggagcagtgg ctgccacctc ttcccagagc
     3421 gatccggtga gcagggcaca gaacgacgag gtgccccggt gggccgcggc ctgcgcagca
     3481 gccagg
//
LOCUS       DASTXO010000292         2185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1675953, whole genome shotgun sequence.
ACCESSION   DASTXO010000292 DASTXO010000000
VERSION     DASTXO010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1675953"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VFU96_06295"
     CDS             <1..820
                     /locus_tag="VFU96_06295"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5112911.1"
                     /translation="GAMADLVTSDGVQLHYQETGSGSPLVFTAGWAMDGTWWRHQLPL
                     ATRHRIVVFDPRSQGQSEKVVRGLRMGRQAADLRELLDMLDLSDVTLVAHSRSTSIAL
                     AYWELFGAHRLSRLVLIGVTPSMSRREDWEWGFMMDPIAFQDLILADHEAVVRQVITD
                     LLLTAPPPDEVEDMVRNTMLTPPLAGARMLEDHGVIDWRDMLGTISIPTLVCVGRFDR
                     NAPLPAAEHVAHSVQQGRLVIFEHSAHVPFYEEPDRFNEVLERFINEHRESGAR"
     gene            complement(846..>2185)
                     /locus_tag="VFU96_06300"
     CDS             complement(846..>2185)
                     /locus_tag="VFU96_06300"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5112912.1"
                     /translation="SMDPNVGDHERGDVLIEGETITRIGPSLQAESASEVTTIVDAEG
                     CIVMPGFVDTHRHMWQGQLRRMIPNVDISMYLGLRNAFAVQYEPRDNYAGTLAIALGA
                     LYSGITSIQDYAHNTRSSSHADAEVEALTTAGIRAVYACAPPEAGEWERQWPEDLSRL
                     NDELAGHPLMTLRMGQRCFSDEDNLTPERIRIARDLGIGMTLDPVAWEEGTNAILDVA
                     SAGLLGPDLTFVHCFDLSAEAWKSMGDADVRVSLSPFVDELLGWGPEGLPTVQRALDV
                     GIDPGLSVDIETTVPCDVFTQMRALLAVHRMRGSLGNTEVERPQMTAKEALAVATTHG
                     ARTIGLEDVCGTLTPGKKADVVLIDCSTPNIFPLSNAYGTVALGADVNAVRAVLVAGE
                     FRKWGDHLVGVDLAEIHEMIETSRDSLCAKVGFEIDLFADYPSIDLGTHTLRM"
BASE COUNT          386 a          715 c          681 g          403 t
ORIGIN      
        1 cggggcaatg gctgacctgg tcaccagcga cggggtgcag ctgcattacc aggaaaccgg
       61 ctccgggtcg ccgctggtat tcaccgctgg ctgggccatg gacgggacct ggtggcgaca
      121 ccagctcccc ctggcgacga ggcatcgaat cgtggtcttc gacccgcgct cccaggggca
      181 gtccgaaaag gtggtccgcg ggctgcggat gggacgacag gccgccgacc tgcgtgagct
      241 gctcgacatg ctcgacctga gcgacgtcac cctggtcgcc cattcccggt ctacgagcat
      301 cgccctggcc tactgggagc tgttcggcgc ccatcgcctg agccgactcg tgctcatcgg
      361 ggtcaccccg agcatgtcga ggcgggagga ctgggaatgg ggattcatga tggaccccat
      421 cgccttccag gacctgatcc tcgccgacca cgaggcggtc gtgcgccagg tgatcaccga
      481 tctgctcctc accgctcccc cacccgacga ggtggaagac atggtgcgga acacgatgct
      541 gaccccaccc ctggccgggg cgcgcatgct cgaggaccac ggtgtcatcg actggcgtga
      601 catgctcggg acgatcagca tcccgacgct ggtctgcgtc gggcgattcg accgcaacgc
      661 gccgcttcca gccgcagaac atgtcgccca ttcggtgcag cagggtcggc tggtcatctt
      721 cgaacacagc gcccatgtgc cgttctatga ggaaccggac cggttcaacg aggtcctcga
      781 gagattcatc aacgagcacc gtgaaagtgg cgctcgatga ttcccccagg gcacagccct
      841 catcctcaca tgcgcagagt gtgtgtcccg aggtcgatgc tcgggtagtc ggcgaacaag
      901 tcgatctcga agccgacctt ggcacaaagg ctgtcccgcg aggtttcgat catctcgtgg
      961 atctcggcga gatcgacccc gacgagatga tcgccccatt ttctgaactc cccagcgacc
     1021 agcacggccc gcaccgcatt cacgtcggca cccagggcga cagtgccata cgcattgctg
     1081 aggggaaaga tattgggagt cgagcaatcg atgaggacga cgtctgcctt cttcccggga
     1141 gtgagtgtgc cgcagacgtc ttcgaggcca atggttcgcg ctccgtgcgt cgtcgcgacg
     1201 gcgagcgctt ccttggccgt catttgcggg cgctcgacct cggtgttgcc cagcgagccc
     1261 cgcatgcgat gcacggcgag cagcgccctc atctgggtga acacatcgca ggggacggtg
     1321 gtctcgatgt ccacactcaa ccctgggtcg atcccgacgt cgagggcacg ctgcaccgtc
     1381 ggcaatccct ccgggcccca cccgagaagc tcatcaacga atggcgacag ggagactcga
     1441 acatcggcgt cacccattga cttccaggcc tcggctgaca gatcgaagca gtggacaaag
     1501 gtgaggtccg ggcccagcaa cccggcggac gccacgtcga gaatggcgtt cgtgccttcc
     1561 tcccatgcga ccgggtcgag ggtcatgccg atgcccaggt cccgggcaat gcggatcctc
     1621 tcgggtgtga ggttgtcttc atcggagaaa caccgctgcc ccatccgcag agtcatcaat
     1681 ggatgtcctg ccagctcatc gtttagacgc gacagatcct caggccattg acgctcccac
     1741 tctccggcct ctggaggagc acaggcgtag acagctctga tcccggccgt ggtcagtgct
     1801 tccacttccg catctgcgtg gctcgacgac cgggtgttgt gcgcatagtc ctggatgctg
     1861 gtgattccgc tgtagagagc cccgagggcg atagcgaggg ttcccgcgta gttgtctctc
     1921 ggctcgtatt ggacggcgaa cgcatttcgc agcccgaggt acatcgagat gtctacgttg
     1981 ggaatcatcc tccgcagttg gccctgccac atatggcggt gggtgtcgac gaacccgggc
     2041 atcacgatgc atccctcggc gtccacgatc gtcgtgacct cgctcgccga ttcagcctgg
     2101 aggctcggcc caatccgggt gatcgtctcc ccttcgatca ggacgtcgcc tctctcgtgg
     2161 tcgcccacgt tggggtccat cgaga
//
LOCUS       DASTXO010000293         2021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1565281, whole genome shotgun sequence.
ACCESSION   DASTXO010000293 DASTXO010000000
VERSION     DASTXO010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565281"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="VFU96_06305"
     CDS             <1..566
                     /locus_tag="VFU96_06305"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEU5112913.1"
                     /translation="SMKDHLCEACAEHYDQVRKSLEGLEVPFVEDARLVRGLDYYTRT
                     AFEYLATDLEAAQNAVGGGGRYDGLAESIGGRRAPGVGFALGVDRIVLASTREPEGRV
                     DVYLVSESGPVEALIAASMLRREGLRVELDTEGRKVEAQFKSASRLEARAVVVLSGDR
                     DEVEVRIEGERARMPIASVAKWLEGRL"
     gene            566..>2021
                     /gene="aspS"
                     /locus_tag="VFU96_06310"
     CDS             566..>2021
                     /gene="aspS"
                     /locus_tag="VFU96_06310"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEU5112914.1"
                     /translation="MRSPAGSLRPSDIGTSVSISGWVQRRRDHGGIAFIDIRDASGVV
                     QVVADPTEHEMVPDLKMEYCISVTGVVRARPEGTENPDLPTGAVEVGVDDLVVLSEAD
                     TLPFMIDDRVEIDERARLRYRYLDLRRPRMGANLRARSRATAAIRRTLDEQGFLEVET
                     PTLINSTPEGARDMLVPSRLREGEFYALPQSPQLFKQLLMIAGVERYFQIARCYRDED
                     FRADRQLEFTQLDLEGSFWDRDDVLDTIESVLVAVVEELRGAEIARPFPRLTWQESMD
                     RYGTDKPDVRFGAEIVDLSEVFDGSEFGVFGDTLKGGGTIRGINAGHQEWSRARADAM
                     TERAKELGGKGLVWMVAEEDGSLRSPVAKFLDERELAGVREALAVKPGDVLLIAADRW
                     RRAVEVLGTLRNDLLRPSDHDDLSFLWVIDFPLFEEAEDGSITFSHHPFTSPASLDEM
                     RDNPTGAVSKAYDAVLNGVELGSGSVRIHDPDVQR"
BASE COUNT          361 a          600 c          708 g          352 t
ORIGIN      
        1 cctcgatgaa ggatcatctc tgcgaggcgt gcgccgagca ctatgaccag gtgaggaagt
       61 cgctcgaggg tctcgaagtc ccgtttgtcg aggatgcgcg gctggtacgc gggctcgatt
      121 actacacgcg cacggcgttc gaatatctcg ccaccgacct cgaggcggcc cagaacgctg
      181 tcggaggcgg tggacgctat gacgggctgg ccgaatcgat cgggggcagg cgcgctccag
      241 gcgtcggctt cgcccttggc gtggatcgga tcgtccttgc ctcgaccagg gaacccgagg
      301 ggcgggtcga cgtctatctg gtctcggagt cgggaccggt cgaggcgctg atcgctgcgt
      361 cgatgttgcg acgagaaggg ctccgggtcg agttggacac agaagggcgg aaggtggagg
      421 cccagttcaa gtcggcaagt cgcctcgagg cccgtgccgt tgtggtgctg tccggagaca
      481 gagacgaggt cgaggtgcgg atcgaaggcg aaagggcgag aatgccaatc gcctcggtgg
      541 caaagtggct ggaagggcgt ctctgatgcg aagccccgca ggatcgttgc ggccatcgga
      601 catcgggacg tcggtgtcga tctcgggatg ggtccagcgg cgtcgcgacc acggtgggat
      661 cgcattcatc gacatccggg acgcctccgg cgtcgttcag gtcgtggctg accccaccga
      721 gcacgagatg gtccccgacc tgaaaatgga gtactgcatc tccgtgaccg gtgtcgtcag
      781 ggcgcgcccg gagggaaccg agaatcccga cctgcccacg ggagcggtgg aggtcggtgt
      841 cgacgatctg gtcgtcctct cggaggccga cactctgccg ttcatgatcg atgacagggt
      901 ggagatcgat gagcgagccc ggctccgcta ccggtacctc gacctgcgtc gcccgcggat
      961 gggagcgaac ctgagggctc gctcacgggc caccgcggcg atacgacgca cgctcgacga
     1021 gcaggggttt ctcgaggtcg agacccccac tctgatcaac agcacgcccg agggggccag
     1081 agacatgctg gtgccctccc ggctgcgtga aggagagttc tacgccctac cccagtctcc
     1141 ccagctcttc aagcagctgc tgatgatcgc cggcgtggag cggtacttcc agatcgcccg
     1201 ctgctaccgg gacgaagact tccgggcgga ccgtcagctc gaattcaccc aactggatct
     1261 cgaagggtcg ttctgggacc gcgatgacgt cctcgacacg attgagagtg tgcttgtcgc
     1321 cgtcgtcgag gagttgcgtg gagccgagat agcccggccc ttcccccgtc tcacctggca
     1381 ggagagtatg gatcgctacg gcacggacaa gcccgacgtc agatttggcg ccgagatcgt
     1441 cgacctttcg gaagtgttcg acgggagtga gttcggagtg ttcggcgaca ccctgaaggg
     1501 cggagggacc attcgtggca tcaatgccgg gcatcaggag tggtcccggg cccgggcgga
     1561 tgcgatgacc gaacgggcca aggagctggg cggaaaaggg ctggtgtgga tggtcgccga
     1621 ggaagacggg tcgctccgat caccggtggc caagttcctc gatgagcgag agctggctgg
     1681 ggtccgagag gccctggcgg tgaagcctgg tgacgttctc ctgatcgccg ccgatcgctg
     1741 gaggcgggct gttgaggtcc tgggaacact acgaaacgac ctgctccggc cgtcagatca
     1801 cgacgatctg tccttcctct gggtgatcga cttcccactg ttcgaagaag ctgaggacgg
     1861 cagcatcacc ttctcccacc atccattcac atcaccggcc tcactcgatg agatgcggga
     1921 caacccgacc ggggcggtct ccaaggcata cgacgcggtg ctcaacggcg tcgagctggg
     1981 ctcgggctcg gttcggatcc atgatccaga cgtgcagcgg c
//
LOCUS       DASTXO010000294         2298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_607804, whole genome shotgun sequence.
ACCESSION   DASTXO010000294 DASTXO010000000
VERSION     DASTXO010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607804"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VFU96_06315"
     CDS             <1..671
                     /locus_tag="VFU96_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEU5112915.1"
                     /translation="QVADDARWKFGKPPASNANYAWIQHFIHHLAPDGRAGFVMANGS
                     LTSNQSGEGTIREAIVRDDLVDCIVACPGQLFYTTQIPVCLWFLDRNKKSGGERDRGG
                     ETLFIDARQMGQKISRTQIEFSEEEIARIADTYHAWRGTTSDTYEDEKGFCAAVTLDE
                     IEKHGFALTPGRFVGAPESEEDEIEFEEKMAGLVDRLADELAESERLTGEVKKALGSV
                     GYDV"
     gene            661..1950
                     /locus_tag="VFU96_06320"
     CDS             661..1950
                     /locus_tag="VFU96_06320"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU5112916.1"
                     /translation="MTFDQIPADWVMATLGEVATDGGLVGGPFGSMLGRKDYVADGVP
                     VIRGTNISDSGRFKLDDLVFVTQEKVTQDLLRNTAAPGDIVLTQRGTLGQAGVIPDDT
                     YPLWVVSQSQERLRVDRAKADRDFVYFVLRSPAMVNEIARRAITTGVPHINLGITQEL
                     PIPLPPLEEQHRIALILNYFDDKIENSQRIAEILEEIAGTLFKARFVDFVDHDDLVES
                     EIGWIPQEWKVASLSEITRVLTRGRGPAYVDEGGVQVLNQKCIRDGRVSFELARRNDE
                     DRRSSTDRRVELGDVLINSTGVGTLGRVAQVSWLPEATTIDSHVTMVRADDTVTEQDY
                     LAMALLMRQGDLERLGHGSTGQTELTRSRLAEFSIPVPPMDEQRALSKSSGPIRGARA
                     ALERESLTIATLRDALLPKLISGQIRVPATGPDEPAA"
     gene            1947..>2298
                     /locus_tag="VFU96_06325"
     CDS             1947..>2298
                     /locus_tag="VFU96_06325"
                     /inference="COORDINATES: protein motif:HMM:NF016220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HEU5112917.1"
                     /translation="MTPPPPGNSKEDIYAEQPALEWLAELGWEVRNGPDLGPGGAAPE
                     RETWRDVVLIRRLRSAVAGLNPELPEDAVEYVVQQVLTTTSPSVIDDHADFHRLLVDR
                     VPVTYEDGNGIERTV"
BASE COUNT          464 a          684 c          731 g          419 t
ORIGIN      
        1 cccaggtcgc cgacgacgcc cgctggaagt tcggcaagcc ccccgcctcg aatgccaact
       61 acgcctggat ccaacacttc attcaccacc ttgcacccga cggacgcgcc gggttcgtga
      121 tggcgaacgg gtccctgacc tcgaaccagt ccggtgaggg aacaatccgc gaagcaatcg
      181 ttcgcgacga tctcgtcgac tgcatcgtcg cctgccccgg ccagctcttc tacacgaccc
      241 agatcccagt gtgcctctgg ttcctcgacc gcaacaagaa gtctggcggc gagcgcgacc
      301 gtggcggcga gacactcttc atcgacgccc ggcagatggg gcagaagatc agtcgcactc
      361 agatcgagtt cagcgaggag gagatcgcgc gcatcgccga cacgtaccac gcctggcgcg
      421 ggacgaccag cgacacgtat gaagacgaga aaggcttctg tgccgcagtg actctcgacg
      481 agatcgagaa gcacggcttc gcgctgaccc ccggccgctt cgtcggtgcg ccagagtccg
      541 aggaggacga gatcgagttc gaggagaaga tggcgggcct cgtcgatcgg ctagccgacg
      601 agctggccga gtccgagcgc cttacgggtg aggtgaagaa ggcgctgggg tctgtcggat
      661 atgacgtttg atcaaattcc ggccgactgg gtaatggcaa cgctcggtga ggttgcaact
      721 gatggcggat tggtcggtgg accctttggg tccatgcttg gacggaagga ttacgttgcc
      781 gatggcgtcc cggtcattcg tggcaccaac atcagtgact ctggacggtt caagcttgac
      841 gaccttgtat ttgtgaccca ggagaaggtc acccaggact tgcttcgcaa caccgcagcc
      901 ccgggagata tcgtcctgac acagcgaggc actctaggcc aagctggcgt gattcccgat
      961 gacacatatc cgttgtgggt ggtttcgcag tctcaggagc gtctccgcgt ggaccgggcc
     1021 aaggccgacc gggacttcgt gtacttcgtg ctccggagcc cggcgatggt caacgagatt
     1081 gctcgtcggg ccataaccac gggcgttccg cacataaatc tcggcattac ccaggaactc
     1141 ccgataccac tgccacccct tgaggagcag caccggattg ccttaatcct taactacttc
     1201 gacgacaaga tcgagaacag ccagcgaatt gcagagattc tcgaggagat cgcagggacg
     1261 ctattcaagg cccgcttcgt cgacttcgta gatcacgacg atctcgtcga aagcgagatc
     1321 ggatggattc cgcaggagtg gaaagtggca tcgctatctg aaatcacccg tgtgcttact
     1381 cgcggtcggg gacccgccta cgttgatgag gggggtgttc aggtcttgaa ccagaagtgt
     1441 attcgtgacg gccgcgtctc gttcgagctg gcccgtcgca acgacgagga caggcgaagt
     1501 agcactgatc gccgggtaga gttgggtgat gtactgatta actcaacggg cgtgggcact
     1561 cttggccgcg tggctcaagt gagctggctc cccgaggcaa cgactatcga cagccacgta
     1621 acgatggtca gggccgacga caccgtcacg gaacaggatt atctggcaat ggctttgctc
     1681 atgaggcaag gtgacttgga gcgccttggg cacgggagca ctgggcaaac ggagttgacg
     1741 cgttcgcgtc ttgctgagtt ttcgattcca gttccgccca tggacgagca acgggcactc
     1801 tccaagagta gtggaccaat ccgcggcgcc agagccgcac tggagcgtga aagccttacc
     1861 atcgccaccc ttcgcgacgc gctgcttcca aagctcatct ccggtcagat tcgcgttccc
     1921 gccaccggtc cggacgaacc cgcggcatga ccccgccgcc gcccggcaat tcgaaggagg
     1981 acatctacgc cgagcaaccg gcgcttgagt ggctggctga gctcggctgg gaggtaagga
     2041 atgggccgga tcttggccca gggggcgcag cgccggagcg tgaaacctgg cgagacgtcg
     2101 tcctgatcag gcgcttgcgc tcggctgtcg cgggcctcaa cccggagctg ccggaggacg
     2161 cggtggagta tgtggttcag caggtgctga cgaccacgtc gccgagcgtg atcgacgatc
     2221 acgccgactt ccaccgcctg cttgtagaca gggttccggt tacctacgaa gacgggaacg
     2281 ggatcgagcg gacggtgc
//
LOCUS       DASTXO010000295         3292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1602043, whole genome shotgun sequence.
ACCESSION   DASTXO010000295 DASTXO010000000
VERSION     DASTXO010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602043"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..1187
                     /locus_tag="VFU96_06330"
     CDS             105..1187
                     /locus_tag="VFU96_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012801473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEU5112918.1"
                     /translation="MEPDLYYGSYLRLDKLLDSQELESTKAGSTVHDEMLFIVVHQSY
                     ELWFKQILWEMDEVRRIFGQTVVDEADMGTAVSLLRRITEIQKLLIQQVSVLETMTPL
                     DFLDFRDYLFPASGFQSAQFRLVENKLGLRPGDRMKLGGATYTSRFSPEDTESVAASE
                     QEPSVFDLVERWLERTPFLDHKDFSFWDAFQRAVKERLEADLAVVESNPRLSADEKAA
                     QAKDFETTMEQYEALFDPAKHAEQQAAGKHRLSHRAFQAALFITLYRDQPALQEPFRL
                     LELLMDIDEGFTTWRYRHAQMVLRMIGRRIGTGGSAGAKYLEKSAERSRIFGDLYNLS
                     TFLIPRADRPPLPAGIVESMRFKYEG"
     gene            1184..2317
                     /locus_tag="VFU96_06335"
     CDS             1184..2317
                     /locus_tag="VFU96_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5112919.1"
                     /translation="MNLKPGFSRFLGARPDRLHFAAHSHHPWPDVSFEAQQRAWLDAA
                     EMMDDKWDHIFGKVIPDARNRAAAILGIADPASLTFGPNTHSFLLRIFSCLEPPVRVL
                     STDAEFHSFTRQSRRWEEAGLAIVDRIPTEPYDTFADRFTREARAGVHDLIYLSQVFY
                     DSGFVVPDLDQIVSSVPENRTFIVIDGYHAFMARPMNLGAIQDRVFFLAGGYKYAMSG
                     EGACFLHSPPGYAPRPLDTGWYAGFGQLETGVSATIAYGNDGSRFAGATFDPSGVYRL
                     GAVLGWLDSEGATPTRIHDHAIALQERFLAGAPDLGDLLVGDAAQRGNFLTFRSGRAD
                     VIYEHLHERGVIADFRADRLRIGFGVYQDEEDVDRLLDVQVSL"
     gene            complement(2314..2709)
                     /locus_tag="VFU96_06340"
     CDS             complement(2314..2709)
                     /locus_tag="VFU96_06340"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017584560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU5112920.1"
                     /translation="MSAHQFLNPTGLAPARGFSHGAVPAKGKTLYIAGQTGHHEDMTL
                     DDDLVEQFAHACRSVAAVITEAGGAPTDLVSMTIYTTDVPGYRDRLGPIGEAYRRVFG
                     KHFPPMALIGVAELFDPEAKVELVGVAVV"
     gene            complement(2706..>3292)
                     /locus_tag="VFU96_06345"
     CDS             complement(2706..>3292)
                     /locus_tag="VFU96_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU5112921.1"
                     /translation="IAPLLEMDARGLLEFTRMTGAVVERIRRLPIPVIAAVNGVAAGA
                     GAVIALAADLRILAESASFSFLFTRVGLAGADMGSAYLLPRVVGLGRATELLLLGESV
                     DAERAVGIGLATRVVADGDLSASARDLAQDLASGPALAYGATKVLIGRELDMDLGSAI
                     ELEAFTQALMMTTEDHAEFYRAFTEKRPPEWRGR"
BASE COUNT          589 a         1098 c         1007 g          598 t
ORIGIN      
        1 ggcccgggag ctgtgggaca agtcggacga atggtgcgga tgagcgaaag cgatctgtcg
       61 gccgaccggg ggatcgaagc accgcgccgg gtaccgtccg ggtcatggag cccgacctct
      121 actacgggag ctatctgaga ctggacaagc tcctcgactc ccaggagctg gagagcacca
      181 aggcagggag caccgttcat gacgagatgc tcttcatcgt ggtccatcag tcctacgagc
      241 tctggttcaa gcagatcctg tgggagatgg acgaggtgag acggatcttc gggcagacgg
      301 tcgtcgacga ggctgacatg ggcactgcgg tgtcattgtt gcgacgcatc accgagatcc
      361 agaagctgct catccagcag gtctcggtgc tcgagacgat gacgccactc gatttcctcg
      421 acttcaggga ctacttgttc ccagcctcgg ggtttcagtc ggcgcagttc cggctggtcg
      481 agaacaagtt gggcctccgc ccaggcgacc gcatgaagct gggtggcgcc acttatacct
      541 cccgcttcag ccccgaggac acggagtcgg tggcagccag cgaacaggag ccgtccgtgt
      601 tcgacctggt cgagcgatgg ctggagcgaa ccccattcct cgaccacaag gacttctcct
      661 tctgggacgc cttccagagg gcggtgaaag agcgtctgga ggccgatctg gccgtggtgg
      721 agtccaaccc gagattgtcc gccgatgaga aggctgccca ggcgaaagac ttcgagacca
      781 cgatggagca atacgaagcc ttgttcgacc ccgccaaaca tgccgagcaa caggctgcgg
      841 gcaaacaccg cctctcccat cgtgccttcc aggcggccct cttcatcacg ctgtatcggg
      901 accagcccgc gttgcaggag ccgttccggc tgctcgagtt gctaatggac atcgatgagg
      961 ggttcaccac ctggcgctac cgccacgccc agatggtcct ccgcatgatc gggcggcgga
     1021 tcgggaccgg aggctccgcc ggagccaagt acctggagaa gtcggcggag cggagccgga
     1081 tattcggcga cctctacaac ctctccacgt tcctcatccc aagagccgat cggccccccc
     1141 tgcccgcggg catcgtggag tcgatgcggt tcaagtacga aggatgaatc tcaagcccgg
     1201 attctcccgg tttctcgggg ccaggcccga tcgtcttcat ttcgcggctc attcccatca
     1261 cccctggccc gacgtctcat tcgaagctca gcagcgtgcg tggctcgacg ctgccgagat
     1321 gatggatgac aagtgggacc acatcttcgg gaaggtgatc cccgatgcgc gcaaccgggc
     1381 tgccgccatc ctcgggatcg ccgacccggc aagtctcacc ttcggcccca acacccattc
     1441 ctttctcctc aggatcttct cgtgtctgga gccaccggtg cgtgtcctct ccaccgacgc
     1501 cgagttccac tcgttcacgc gccagtcacg gcgttgggag gaggcggggc tcgccatcgt
     1561 cgaccggatc ccgaccgagc catacgacac cttcgccgac cgtttcaccc gcgaagcgag
     1621 ggcgggcgtc cacgacctca tctacctgag ccaggtcttc tacgactccg ggttcgtcgt
     1681 gcccgatctc gaccaaatcg tctcatcggt gccggaaaac cggaccttca tcgtcatcga
     1741 tgggtaccac gccttcatgg cccgacccat gaacctcggc gccatccagg atcgggtgtt
     1801 cttcctggcc ggcgggtaca aatacgcgat gtccggtgaa ggggcctgtt tcctccattc
     1861 cccaccgggt tatgcccccc gccctctcga caccggatgg tatgcgggat tcggccaact
     1921 cgaaaccggg gtcagcgcga cgatcgccta cggcaacgat ggctcgcgtt ttgccggtgc
     1981 caccttcgac ccgtccggtg tgtatcgatt gggggcggtg ctcggctggc tcgactcgga
     2041 aggagctacg ccgacccgga tccacgatca cgccatcgcc ttgcaggagc gattcctcgc
     2101 cggcgctccc gatcttggcg acctgctcgt cggtgacgcc gctcagcgag gcaacttcct
     2161 cacctttcgg tcaggtcgag ccgatgtgat ctatgagcac ctccatgagc gaggtgtgat
     2221 cgccgatttc cgggccgacc ggcttcgcat cgggtttggc gtctatcagg acgaagaaga
     2281 tgtcgaccgg ttgttggatg tgcaggtgag tctctagacc acggcgacgc cgaccaattc
     2341 gacctttgcc tcgggatcga acagctccgc aaccccgatc agggccatcg gggggaaatg
     2401 cttgccgaac acccgacgat aggcctctcc gatggggccg agacggtccc ggtacccggg
     2461 cacatccgtc gtgtagatcg tcatcgagac cagatcggtc ggagcccccc cggcctccgt
     2521 gatgaccgcc gccaccgatc ggcaggcatg ggcgaattgc tcgacgaggt cgtcgtcgag
     2581 cgtcatgtct tcgtgatgac cggtttgccc ggcgatgtag agcgtctttc ccttggcggg
     2641 taccgcccca tgggaaaacc cacgcgccgg ggcaagaccc gttggattca ggaattggtg
     2701 cgcgctcatc ggcctctcca ttccggtggc cgcttctcgg tgaaggcccg gtagaactcg
     2761 gcgtggtctt ccgtggtcat catcaatgcc tgggtgaacg cctccagctc gatggccgag
     2821 ccaaggtcca tgtccagctc acgcccgatc agcaccttcg tcgccccgta ggccagagcc
     2881 ggtcccgatg caaggtcctg tgccaggtct ctcgccgagg cagacaggtc gccgtcggcg
     2941 accacccgtg tcgccaaccc aatgccgacc gcccgctccg cgtcgacact ctctcccagg
     3001 agcaatagct cggtggcgcg gcccagaccg accacccgag gaagcagata ggccgagccc
     3061 atgtccgccc cggccaggcc gaccctggtg aagagaaagc tgaaggaggc cgactcggcc
     3121 aggatcctga gatccgcggc cagggcgatg actgccccgg ctcccgctgc gaccccgttg
     3181 accgccgcta tcaccgggat gggaagccgt cggatcctct ccaccaccgc gccggtcatc
     3241 cgtgtgaact cgagaagccc tctggcgtcc atctcgagca acggggcgat ga
//
LOCUS       DASTXO010000296         2848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_423873, whole genome shotgun sequence.
ACCESSION   DASTXO010000296 DASTXO010000000
VERSION     DASTXO010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423873"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1461)
                     /locus_tag="VFU96_06350"
     CDS             complement(<1..1461)
                     /locus_tag="VFU96_06350"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5112922.1"
                     /translation="MVEVPPIPDLELALRVVDSVQDHPQRARDEAGLLVARTDIDRET
                     LAVAWWGLGLAARQLNDLEEADSALRTALGIAMEGGLTRRVGQIRSSLALVLLYGGDT
                     QAALDETKLATVNLSGPDLARNEMQIGLILQRLGHLDEALVHYRTALTGLRRSGDRLA
                     EARLLSNRGVLHGYRGELDLGVSDLEAARIIAEDLGQKLIVAASAHNLGFLEGRRGDV
                     PAALAWFDRAEAAYEALGRPPGMTEVLWANRGELLLTVGLYSEAEQAVRAAIAGLERI
                     DNKTDLSETRLLAAEVALANRRPNEALEAASVAASEFTDQDRLAWRLLADYAALRARF
                     EAHISDGATASDAARLAGGLAAIGLSSESQHCHLLAGRIAMRDGSLENARRELAAASG
                     ARRRGTSLDRAKAWYAEALLRQAEGNVAGSSRAIEAGFRAIQDYRLTLGAADLRSYAA
                     SHGLDLAVLAIEQGVASGSPWRVLRAVESWRAESSQV"
     gene            1526..>2848
                     /locus_tag="VFU96_06355"
     CDS             1526..>2848
                     /locus_tag="VFU96_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU5112923.1"
                     /translation="MPIDPEAVAASIEARFEERNIAVSAAGGFAYRRDEIIVTVPAGT
                     SSDSLPIVNGEPIADPQNHGGEFDHHLFQVADPVAEIQSVRDQASRASFNHVLFGEPV
                     YLGNPVYLGNPVYLGNPVYLGNPVYLGNPFARNCARPAAEPNLWPEPTGCAGEPPNVL
                     VLDTGLATVPCETGRMPANERLRDHAVVHPAWTDANALAATPIDISRTDEDEKGMDGD
                     GHLDRQAGHGTFIAGIIRRHAPDAKILTQGVISSFGDGDDWDISAGLAEALARDRVDV
                     VNMSLGCYTDDDLPPLTPEGLPCLSDAVARAKDQGAVVVAAAGNNGSCRKFWPAALDG
                     VISVGATFCGRRAWFSNFGPWVTACAPGVDVISTFFEHSGPASSVAGVDPDEYAGWAM
                     WSGTSFAAPQVAAVIAKTMCETGGTADQAAAIVLRSGVDIEDLGIHIVA"
BASE COUNT          495 a          921 c          923 g          509 t
ORIGIN      
        1 aacctggctg ctctccgctc gccatgactc cactgccctg agcacccgcc agggcgatcc
       61 cgacgcgaca ccttgttcga tggcgaggac tgccaggtcg agaccgtggg acgcggcgta
      121 tgaacgaaga tctgccgccc cgagtgtcag gcggtaatcc tggatcgccc tgaatccggc
      181 ttcgatagcc cggctcgagc ccgctacgtt gccctcggct tgccggagca gggcctcggc
      241 gtaccaggct ttggcccggt ccaaggacgt tcccctccgc cgggcgccgg aggcggcagc
      301 caactcgcga cgggcgttct cgagtgaccc atcacgcatg gcgatccgcc ctgccaacag
      361 atggcaatgc tgcgactccg aggaaagtcc gattgcggcc aaacctccgg cgagtcgggc
      421 tgcgtccgat gcagtcgcgc cgtctgagat gtgggcttcg aaacgagctc gaagcgcggc
      481 gtaatcggcg agcagccgcc acgccagccg atcttgatcg gtgaattccg aggcggcgac
      541 ggaggcggct tcgagcgcct cgtttgggcg ccgattggcg agggcgacct cggcggcgag
      601 gagacgggtc tcagagaggt cggtcttgtt gtcgattctc tccaagccgg caatggctgc
      661 tctcacggcc tgctcggcct ccgagtacaa cccaacggtg aggaggagct cgccgcgatt
      721 ggcccagagc acctcggtca ttcccggtgg gcgtcccagc gcttcatatg ccgcctcggc
      781 tcgatcgaac caggcaaggg ctgcaggcac gtcacccctt cttccttcga ggaaaccgag
      841 gttgtgtgca ctggctgcga cgatcagctt ctgacccagg tcttcggcga tgatccgggc
      901 ggcttcgaga tctgacactc ccagatcgag ctcaccccga tagccgtgaa ggacgccacg
      961 gttggagagc agacgcgcct cggcaagccg gtccccgctg cgtctgagcc cggtcagggc
     1021 ggtgcgatag tggacgaggg cctcgtccag gtgccccagc cgctgcagga tcagtccaat
     1081 ctgcatctcg tttcgggcca ggtccgggcc cgaaagattg accgtggcca gcttcgtctc
     1141 atcgagagct gcctgcgtat ccccgccgta gagaaggacc agagccagac tcgacctgat
     1201 ctggccgacc cgtctggtga gaccgccttc catcgcaatg cctagggcgg ttcgcagagc
     1261 gctatctgcc tcctccaggt cgttgagttg tctggcggcc agcccaaggc cccaccacgc
     1321 tacggccagt gtctcccgat cgatgtcggt ccgggcgacg agcaatccgg cctcatcccg
     1381 tgctcgctgg gggtgatcct gcaccgagtc gacgacccgg agcgcgagct cgaggtcggg
     1441 gatcggaggc acttcgacca tcggcccgag actaggtttc ggcggtcgcg ctgggagcgc
     1501 gtttcgatga aagtggggca agctgatgcc gatcgatcct gaggctgtcg cggcgagcat
     1561 cgaggcgcgg ttcgaggaac gaaacatcgc ggtgtcggcc gccggcggat ttgcctaccg
     1621 ccgggacgag atcatcgtca ccgttcccgc tggcacgtcg agtgactcgc tacccattgt
     1681 caacggcgaa ccgatcgctg atccacagaa ccacggcggg gaattcgatc atcacctctt
     1741 ccaggtggcc gacccggtgg cggagatcca gtcggttcgt gatcaggcat cccgggcctc
     1801 cttcaatcac gtcctgttcg gcgaaccggt gtacctggga aacccggtgt atctggggaa
     1861 ccccgtctac ctcgggaatc ccgtgtacct gggcaatccg gtatatctgg gcaacccctt
     1921 tgccaggaac tgtgcccgac cagccgccga gcccaatctg tggcctgagc cgaccggttg
     1981 tgccggggag ccacccaacg tattggttct cgacactggc ctggccaccg tgccctgtga
     2041 aacaggtcgg atgccggcca acgagcgtct cagagatcac gccgtggtgc atcccgcttg
     2101 gaccgatgcc aacgcgttgg ccgcaacgcc aatcgatatc tcacgcacgg acgaagacga
     2161 gaagggaatg gacggcgacg ggcacctcga cagacaggct ggacacggca cattcatcgc
     2221 ggggatcatc cggcgccacg cccccgacgc gaagatcctc acccagggtg tcatctccag
     2281 ctttggcgac ggggacgatt gggacatctc agccgggctt gcggaggctc ttgccagaga
     2341 tcgggtcgac gtggtcaaca tgtcgctggg ttgctacacg gacgacgacc ttcccccgct
     2401 gacacccgag gggcttcctt gtctgtctga tgccgtcgcc cgcgcgaaag atcagggtgc
     2461 ggtggtcgtt gcagcggccg ggaacaatgg ctcttgtcgc aagttctggc cggccgccct
     2521 ggacggtgtc atctccgtcg gggcgacgtt ctgcggcaga cgagcatggt tctccaactt
     2581 cgggccgtgg gtgaccgcat gcgctcccgg agtcgacgtg atcagcacct tcttcgagca
     2641 cagcggacct gcttcgagcg tggctggtgt agaccccgat gaatacgccg gatgggccat
     2701 gtggtcgggg acctcctttg cggcaccgca agtggcagcc gtcatcgcaa agaccatgtg
     2761 tgaaaccggg gggactgccg atcaggctgc agccatagtc ttgagatcag gtgtagacat
     2821 cgaggatctc gggatccaca tcgtcgcg
//
LOCUS       DASTXO010000297         2324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1676031, whole genome shotgun sequence.
ACCESSION   DASTXO010000297 DASTXO010000000
VERSION     DASTXO010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676031"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /gene="nusG"
                     /locus_tag="VFU96_06360"
     CDS             <1..815
                     /gene="nusG"
                     /locus_tag="VFU96_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010315508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEU5112924.1"
                     /translation="METPDVDTEVEESPEAESPSAETPQLEVDVAEADAPGGDVDTTQ
                     VDTVELEPVDAEEPTVEESVAAEETEATEESDVEADEAIPTLSASPYDMPGDWYVIHS
                     YSGYENKVKANLETRIQSMHMEDRIFEVHIPMEDMVEFKGGKKQTVSKKVFPGYILVR
                     MTLDDDSWYAVRNTPGVTGFVAGNAQKPTPLSRREVERFLGVQEEEEKAKPRFKPEWE
                     VGEQVRVVTGPFADFNGVIEDMNIDQQKIVVLVNIFGRDTPVELGFEDIQKS"
     gene            840..1268
                     /gene="rplK"
                     /locus_tag="VFU96_06365"
     CDS             840..1268
                     /gene="rplK"
                     /locus_tag="VFU96_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010550349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEU5112925.1"
                     /translation="MGRRRLVTTLKLQLPAGQATPAPPVGTALGPHGVNLMDFVKAYN
                     DATANQRGQIIPVEISIYEDRSFDFVMKTPPAAALLRQAARVDKGSGEPNKEKVGKVT
                     RAQVKEIAETKMPDLNAIDIEGAMKIVEGTARSMGITVEG"
     gene            1347..2063
                     /gene="rplA"
                     /locus_tag="VFU96_06370"
     CDS             1347..2063
                     /gene="rplA"
                     /locus_tag="VFU96_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEU5112926.1"
                     /translation="MAKHGKKYREAAMRYDKQTPYGPEEALELVKTLAPAKFDETVEA
                     VFGLGIDPKKADQLVRGTVSLPHGTGKDVRVAVFAPADKEKEATEAGAAVVGGKELVE
                     KLQAGGELDFDVAIATPDMMAEVGKLGKILGPRGLMPNPKAGTVTPDVAKAVSEFKAG
                     RIEYRNDGYGNVHVPVGKVSFGLDQLSSNLAALSSELLRAKPAASRGRYLQQISLSST
                     MGPGVKVDVGSLEDFAKKAG"
BASE COUNT          493 a          708 c          777 g          346 t
ORIGIN      
        1 agatggagac cccggacgtc gacaccgagg tcgaggagtc gccggaggcc gaaagcccgt
       61 cggcagaaac accccaactc gaggtagatg tcgccgaggc cgatgcacct ggaggcgatg
      121 tcgacaccac ccaggtggac accgtcgagc tcgagcccgt tgacgccgag gagcccaccg
      181 tcgaagagtc tgttgccgcc gaggagaccg aggcgacaga agagagcgat gtcgaggccg
      241 atgaggccat cccgacgctg tcggccagcc cctatgacat gccgggcgac tggtatgtca
      301 tccactccta ctcgggatac gagaacaagg tcaaggccaa tctcgagaca cggatccagt
      361 cgatgcacat ggaggaccgg atcttcgagg tccatatccc gatggaggac atggtcgagt
      421 tcaagggcgg gaagaagcaa acggtctcga agaaggtctt ccccggctac atcctggtcc
      481 ggatgaccct cgacgacgac tcctggtatg cggtccgcaa caccccgggg gtcaccgggt
      541 tcgtcgccgg caacgctcag aagccaacac ctctttcccg cagggaggtc gagcggttcc
      601 tcggcgtcca ggaggaagag gagaaggcca agccacgctt caagcccgag tgggaggtcg
      661 gagagcaggt gcgggttgtc accggcccat tcgccgactt caacggggtc atcgaggaca
      721 tgaacatcga ccagcagaag atcgtggtcc tcgtcaacat cttcggccgg gacacaccgg
      781 tcgagctcgg tttcgaagac atccagaaga gttagtgccc caccggcacg gagagaatca
      841 tgggacgaag acgacttgta accacgctca aattgcagct ccccgccggg caggccactc
      901 cagccccacc ggtcggcacc gccttgggcc cacacggagt caacttgatg gacttcgtga
      961 aggcctacaa cgacgccacg gcgaaccaga gggggcagat catcccggtc gagatctcga
     1021 tctacgaaga ccggtcgttc gacttcgtca tgaagacacc gccggccgcg gcgctgctcc
     1081 gtcaggcggc gcgggtcgac aagggctcgg gtgagccgaa caaggagaag gtcgggaagg
     1141 tgaccagggc tcaggtcaag gagatcgccg agaccaagat gcccgatctc aacgccatcg
     1201 acatcgaagg cgccatgaag atcgtcgagg gcacggcccg atcgatggga atcaccgtcg
     1261 aaggctgaat ctcgacaacg aacgaacggc gcccctggcg ccgccagtgg gaggccgcga
     1321 ggccgcctgg accacaagga gagacaatgg ccaagcatgg caagaagtac cgcgaggccg
     1381 cgatgcggta cgacaagcag accccgtacg ggccggaaga ggccctggag ctcgtgaaga
     1441 ccctggctcc cgccaaattc gacgagacgg tcgaggctgt gttcggcctt ggaatcgatc
     1501 ccaagaaggc agaccagctc gtccgcggca ccgtctcgct cccgcacgga accggcaaag
     1561 acgtcagggt ggcggtgttc gctccagcgg acaaggagaa agaggcaacc gaggccggcg
     1621 ccgccgtggt cgggggcaag gagttggtgg agaagctcca ggccggcggc gagctcgatt
     1681 tcgacgtcgc tatcgccacc ccggacatga tggccgaggt gggcaagctg gggaagatcc
     1741 tcgggccccg cggcctcatg ccaaacccca aggccgggac ggtgactccg gacgtggcca
     1801 aggcagtgtc cgagttcaag gcggggcgta tcgagtatcg caacgacggc tatgggaatg
     1861 tccacgtccc ggtaggcaag gtctcgttcg gcctcgacca attgtcgtcg aatctcgctg
     1921 cgctctcctc ggagctgctc agggcgaagc cggcagcatc gcgcgggcgt tacctgcagc
     1981 agatttccct ttcctcgacc atgggcccgg gggtcaaggt cgatgtcgga agcctggagg
     2041 atttcgccaa gaaggccggc tagggccgcg actcgagtcg ggtaacacag aagagccccc
     2101 cttgggggct cttctctttg tgaaacaggg gggatctccg cgcttcggat tcccgtgggc
     2161 ttagagggct tcggctgcca ggtccaccgt ccgcacgctt ccgacgactc gtctgtggac
     2221 cagggtaggg gctggtctca tctgtcggtt acgcgaccct caggttattt ggggcccgaa
     2281 gagccgggtg ggggcggtca gggaacccac ccggtcacac ggag
//
LOCUS       DASTXO010000298         2608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_350307, whole genome shotgun sequence.
ACCESSION   DASTXO010000298 DASTXO010000000
VERSION     DASTXO010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350307"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1128
                     /locus_tag="VFU96_06375"
     CDS             91..1128
                     /locus_tag="VFU96_06375"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEU5112927.1"
                     /translation="MTSTDPETRRGMPADGIASRATKPAEPGSRLWLYSGDGEDAPVR
                     LDTELPDLGKNQMLWADVDLEVADDLDQLWKNLEIEDLIAGLGKLEEGPALVQHNGIL
                     QLAVTVLLDEFELEPMTLHCLVGPNWVVTLHRGELDLVDEFNKPFHGETRLGDLDGPA
                     FLSLVLDWQVSGYFKIIEGLQGDIDHLDEDLLSKSPEEGSFLDRLQEFRRRVRTLRNT
                     LSAHREVLGLLSHPRSDAVVGAEATEDYQRLEDRLQQAIEAVDTTREMIVGSFDIFMT
                     RTAQTTNDIMKRLTIVSVLLLPTAAIAGIMGMNFKIGIFERPWMFWVTLGLMAVVAGI
                     TLLVAKRNDWL"
     gene            complement(1130..2077)
                     /locus_tag="VFU96_06380"
     CDS             complement(1130..2077)
                     /locus_tag="VFU96_06380"
                     /inference="COORDINATES: protein motif:HMM:NF041045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor RsbA family regulatory
                     protein"
                     /protein_id="HEU5112928.1"
                     /translation="MVITQEGPGFRHEALPYGADATMVDQVTRFLSPALEKGEPVLIA
                     TKADSLSSLEKAFHGHDLVDLTPMEEVGRNPGRLIWLWQDFLDRNGAEERIVWGIGEP
                     IYAGRDAAELAEGQIHERLLNRAFNGSQFALHLGCPYDMSSLPGDVADEMGRSHPFIA
                     NGAGSRDNEGFQPADQEAPPAPLGPALIPDEAFIPFDEQSLGRMRRQLAGEARICGVD
                     ETRIPDLLLAVNEIATNSILYAGAGSLGVWQDSGRIICEVRDQGFIDDDLVGRLRPAV
                     TEEQGRGIWFAHQVADLLQFRSAPSGTQWRVIFDCGSPR"
     gene            complement(2327..>2608)
                     /locus_tag="VFU96_06385"
     CDS             complement(2327..>2608)
                     /locus_tag="VFU96_06385"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEU5112929.1"
                     /translation="GQPCPPVGVVPAIHPIETTRWLDHGDTVVLYTDGYAAPGLHPPE
                     TVELALSKCEPDIPGGLLDQMLDLLFADLAPEGARDDIALVALQATDAV"
BASE COUNT          506 a          829 c          791 g          482 t
ORIGIN      
        1 actccggtcc caaatccacc atctcagcct ccctcgggcg aacgggctcg gtttgcggcc
       61 ctccggacga gggtaagtat ccctcccttg gtgacctcca ctgaccccga aacccgtcgc
      121 ggcatgcctg ccgatggtat cgcgtcgcgt gcaaccaagc ccgctgagcc ggggagtcgt
      181 ctctggctgt acagcggcga cggcgaagac gcccctgtca ggctcgacac ggagctcccc
      241 gacctgggca agaatcaaat gttgtgggcc gatgtcgacc tcgaggtcgc cgacgacctg
      301 gaccagctct ggaagaacct cgagatcgag gatttgatcg cgggtctggg aaaactcgaa
      361 gaaggcccgg ctcttgttca gcacaacggg atcctgcagc tcgcagtgac cgtgctgctc
      421 gacgagttcg aactcgagcc catgaccctg cattgcctgg taggcccgaa ttgggtggtg
      481 acgcttcatc gaggggaatt ggatctggtc gatgagttca acaagccgtt ccatggtgag
      541 actcgcctcg gagacctcga tggcccggcc ttcctgtcgc tggtgctcga ctggcaggta
      601 tccggatatt tcaagatcat cgagggcctt cagggtgata tcgaccatct cgacgaagac
      661 ctgctcagca agtcacctga ggaggggtca ttcctggacc gcctgcagga gtttcgccga
      721 cgggtgcgga cgctgcggaa cacactcagc gcccatcgcg aggtcttggg cttgctcagc
      781 cacccgagat ccgacgccgt cgtaggggca gaggcaactg aggactacca gcgtctcgag
      841 gatcgccttc agcaggcaat cgaggcggtc gacacgactc gggagatgat cgtcggatcc
      901 ttcgacatct tcatgaccag gaccgcccag accacgaacg acataatgaa gcgactcacc
      961 atcgtctccg tgctcctact cccgaccgcg gcgatagctg ggatcatggg gatgaacttc
     1021 aagatcggca tcttcgaacg accctggatg ttctgggtca cgctcggtct gatggctgtg
     1081 gtggccggga taaccctgct cgtggccaaa cgcaacgact ggctctgaat tagcgcggcg
     1141 agccgcagtc gaagatgacg cgccattgtg tgcccgaggg cgcacttcga aattgcaaga
     1201 ggtccgccac ctgatgagcg aaccagatcc cccgtccctg ttcctcggtg accgcagggc
     1261 gaagccgacc aaccaggtca tcgtcgatga atccctggtc gcgcacctcg cagatgattc
     1321 gcccgctgtc ctgccaaact cccaacgagc ccgcccccgc atagaggatg ctgttggtcg
     1381 cgatctcgtt cacagccaac aacaggtcgg gtatccgggt ctcatcgaca ccgcagatgc
     1441 gtgcctcgcc ggccaactgt ctccgcatcc tcccgagact ctgctcgtcg aaggggatga
     1501 aggcttcgtc ggggatcaac gccgggccca agggagccgg gggagcttcc tgatcggccg
     1561 gttggaaccc ctcgttgtcc cgtgatccgg ccccgttggc gatgaatgga tgagaacgtc
     1621 ccatctcatc agccacatcg ccaggcaacg agctcatgtc gtagggacaa ccgagatgca
     1681 gggcgaactg cgatccattg aaggcgcgat tgaggagtcg ctcgtggatc tgcccctccg
     1741 ccagttcggc ggcatcgcgg cccgcgtaga tcggctctcc tattccccaa acgatccgtt
     1801 cctcggctcc gtttcggtcg aggaagtcct gccacagcca gatcagccgc cccgggttgc
     1861 ggcccacctc ttccatcgga gtcaggtcga cgagatcgtg accgtggaag gccttctcca
     1921 gactcgacaa actgtctgcc ttcgtggcaa tcaacaccgg ctcgcccttt tccagagccg
     1981 ggctgaggaa tctggtgact tgatcgacca tggtcgcatc tgccccgtag ggcaaagcct
     2041 catgacggaa tccggggcct tcctgggtga tcaccatgca ccgcgaagat cgagactgag
     2101 agacctgtgc ccccccgcaa agcagaagcg caagccaact ctaaacctgg ccagaggtgg
     2161 atcgcgggga gccgaaaacc ggctgaaccg gtgccccatc agcgagtcga cgaaatcctc
     2221 agctgacggc ggggtgtcgc ctgatagagc agctcggtca cctgtgcatc gtccaccagt
     2281 cgtctcccgt gtcgggtgga gaagagttac ccgtccgggc tgtgtgtcaa acggcgtccg
     2341 tggcctgcaa ggcgaccagc gcgatgtcgt cgcgtgctcc ctccggggcc aggtcggcaa
     2401 acaagagatc cagcatttga tcgagcagac caccgggaat gtctggctca catttcgaca
     2461 gggcgagctc cacggtctct ggtggatgaa gcccgggggc tgcataccca tcggtgtaga
     2521 gaaccactgt gtccccgtga tccagccatc gtgttgtctc gatcgggtgg atcgccggca
     2581 ccacgccgac gggcgggcag ggctgtcc
//
LOCUS       DASTXO010000299         3194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1639315, whole genome shotgun sequence.
ACCESSION   DASTXO010000299 DASTXO010000000
VERSION     DASTXO010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1639315"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VFU96_06390"
     CDS             complement(<1..240)
                     /locus_tag="VFU96_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112930.1"
                     /translation="MPKTDEEGALINLVSKRQLLLALAVVSTLALAPAALAQTEETPT
                     TLPATALTVSTPFPSVAVEPGDQVSFVLTVAAPAQA"
     gene            complement(336..1208)
                     /locus_tag="VFU96_06395"
     CDS             complement(336..1208)
                     /locus_tag="VFU96_06395"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEU5112931.1"
                     /translation="MRLEIRYLNEFNYSEPAWESHNLLRACPANDDHQRLITYRVEVN
                     PRARVHSYTDYWGTRVDEFGIVEPHRDLRIMAESTVETTTPLTPSGEAPVEALDTVRE
                     ELSSFLRPSPHAIWDGLIEETAREVVSGAGGVVDAAAAVSDRVTSTLDYQPGATYVGV
                     DVADVMSQGKGVCQDFAHLGIAMYRSIGIPARYVSGYLYAADQTSAVAPDEPELSVQT
                     HAWLEVFVPGYGWWGLDPTNAQPVGELHVKIGHGRDYQDVMPLRGVYHGGAEHQLGVQ
                     VRISRERLSQMAEQ"
     gene            complement(1205..2158)
                     /locus_tag="VFU96_06400"
     CDS             complement(1205..2158)
                     /locus_tag="VFU96_06400"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEU5112932.1"
                     /translation="MLSRHAEDLYWVGRYVERAEDTARMLDVTYHNLLESPGDGAATT
                     WNELLEVLYVNALYSGPLESSAVSSYLVADRTNPGSIMSAVERARDNARGLRDRISTE
                     LWEAINVFHLDLARADFHLDLDRRAYEVFRNIKSSCQLISGVAAQTMPRDEGYRFMLL
                     GLLLERAEMTCRLLSVRYARLAGGEGQSGFHAWVSVLKSVSAYEAYLKAHDASLDPTD
                     VLQFLLLDQEFPRSVLYCLQQVERLIDRLTGSSRQTSMERAVGRVRAHAEFTDIPTIV
                     DGRLDPFLEELQKEIYEVSDEVEAHFFRAGTDLQLHFQRWA"
     gene            complement(2139..>3194)
                     /locus_tag="VFU96_06405"
     CDS             complement(2139..>3194)
                     /locus_tag="VFU96_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013137417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEU5112933.1"
                     /translation="GIPAPRGSRCVVAGIDIVRDDEGTYRVLEDNLRVPSGISYVLEN
                     RVAMTRILPVGFASHVIRPVDHYGASLLRALRAIAPPNTDDPTVVVLTPGVANSAYFE
                     HAFLARQMGIELVEGRDLLVDDRQVFMRTTKGLKQVDVIYRRVNDEFLDPVVFRQDSL
                     LGVPGLHGAARAGTVTIANAIGNGAVDDKGVYPYLPALIEYYLGEEPILPNVTTYLPW
                     EPDQLEIILRRLDQLVVKPVAEAGGYGIVIGNMADEATLEATAAALRANPRGYVAQEI
                     VQLSTHPTFVDGHLEPRHVDLRPFVLSGDRIEVVPGGLTRVALRKGSLIVNSSQGGGS
                     KDTWVLGNGSGHAVTAR"
BASE COUNT          551 a          997 c         1096 g          550 t
ORIGIN      
        1 ggcctgggcc ggggcggcga cggtgagaac gaagctcacc tggtcgccgg gctcgaccgc
       61 taccgagggg aacggggtgc tgaccgtcag agctgtggcc gggagcgtcg ttggcgtctc
      121 ctccgtctgc gcgagagccg caggggccag agccagcgtg gacaccacgg ccaacgccaa
      181 gagcaattgc ctcttcgaca ccaagttgat cagagctcct tcttcatcgg ttttgggcac
      241 aaacactcga tcgtgacggc agagacccta aaacgatgat aaatccattg agaaaacggt
      301 tcccaatgca gcgcgtcatg cgaatcgggt ttggatcact gctcggccat ctgggagagg
      361 cgctcccggc tgatccgcac ctggacgccg agctggtgtt ctgccccgcc gtgatagaca
      421 ccacgcaacg gcatcacgtc ctgataatcc cttccgtgcc cgatcttgac gtggagctcg
      481 ccgaccggtt gggcgttggt tgggtcgagg ccccaccatc cgtatcccgg cacgaagact
      541 tcgagccagg catgggtctg cacactcagc tccggctcat ccggcgcaac tgccgaggtc
      601 tggtccgctg cgtacagata gccggacaca tagcgggccg ggatgccgat gctgcgatac
      661 atcgcgatgc ccaggtgggc gaagtcctgg cacactcctt tgccctggga catcacgtcg
      721 gccacatcga cgccgacata cgtggcgccc ggctggtaat ccagggtgga ggtgaccctg
      781 tcggagacgg cagcggcggc gtcgacaacc cctccggcgc cgctcacgac ctccctggcc
      841 gtttcctcga tgaggccgtc ccagatggcg tggggtgagg ggcggaggaa gctcgacagc
      901 tcctcccgca ccgtgtcgag cgcctcaacc ggggcctcac cagatggggt caggggcgtc
      961 gtcgtctcga cggtcgactc ggccatgatc cggagatcgc ggtgaggttc gacgatcccg
     1021 aattcgtcga ccctcgtgcc ccagtagtcg gtgtaggaat ggacccgggc cctggggttg
     1081 acctcgaccc ggtaggtgat caaccgctgg tggtcgtcat tggccgggca ggcccggagc
     1141 aggttgtggg actcccaggc cggttcgctg tagttgaact cgttgagata gcggatctcg
     1201 agcctcatgc ccacctctgg aagtggagtt ggagatcggt cccggcacgg aagaaatggg
     1261 cctccacctc atcagagacc tcgtagatct ccttctgcag ctcctcgagg aacgggtcga
     1321 gccgcccgtc gacgatggtg ggtatgtcgg tgaactcggc atgggccctg acccgtccca
     1381 cggcgcgctc catgctggtc tgtcggctcg atccggtgag gcgatcgatc agacgctcca
     1441 cctgttggag gcagtagagg acgctccgtg ggaactcctg gtcgagcagc aggaactgga
     1501 gcacgtcggt cgggtcgagc gatgcgtcgt gcgccttgag ataggcctcg taggcggaga
     1561 ccgacttgag caccgacacc caggcgtgaa acccggattg tccttccccg ccggcgagcc
     1621 gggcataacg cacggagaga aggcggcagg tcatctcggc gcgctccagc aggaggccga
     1681 ggagcatgaa gcggtaaccc tcgtcccggg gcatggtctg ggcggcgact ccgctgatca
     1741 gctgacagct gctcttgatg ttgcggaaca cctcgtaggc ccgtcggtcg aggtcgagat
     1801 ggaagtcggc gcgggccagg tcgagatgga agacattgat cgcttcccac agctcggttg
     1861 agatccgatc ccgcaggccg cgggcgttgt cacgggcccg ctccactgcc gacatgatcg
     1921 agcccgggtt ggtccgatcg gcgaccaggt agctgctgac cgcgctgctc tccagcggac
     1981 ccgagtacag ggcgttgacg tagaggacct cgagtagctc gttccaggtg gtggccgcac
     2041 cgtcaccggg ggattcgagg aggttgtgat aggtcacgtc gagcatgcgg gcggtgtcct
     2101 cggcccgctc gacgtagcgg cccacccagt agagatcctc agcgtgccgt gacagcatgc
     2161 ccgctcccgt tgcccagcac ccatgtgtcc ttggatccgc cgccctgtga ggagttcacg
     2221 atcaacgagc ccttccgcag cgccacccgg gtcaggccgc caggcaccac ctcgatcctg
     2281 tcgccgctga ggacgaatgg gcgcaggtcg acgtggcgag gctcgaggtg cccgtcgacg
     2341 aaggtgggat gcgtggacag ctggacgatt tcctgggcga catagcccct ggggttggcg
     2401 cggagtgcgg cggcggtggc ctcgagggtg gcctcgtcgg ccatgttgcc gatcacgatg
     2461 ccgtaaccgc cggcctcggc caccggtttg accaccaact ggtcgagccg gcggaggatg
     2521 atctccagct ggtcgggctc ccaggggaga taagtggtga cgttggggag gatcggctcc
     2581 tcgccgaggt agtactcgat cagggcgggg aggtagggat acacgccctt gtcatcgacc
     2641 gccccgttgc ctatggcatt ggcaatggtc acggtcccgg cccgcgccgc tccgtggagc
     2701 ccggggacgc cgagcaggct gtcctgacgg aagacgaccg ggtcgaggaa ctcgtcgttg
     2761 acccggcgat agatcacatc gacctgcttg aggcccttgg tcgtgcgcat gaacacctgc
     2821 cgatcgtcaa cgaggaggtc ccgcccctcc accagctcga tgcccatctg ccgggctaga
     2881 aaggcgtgct cgaagtaggc cgagttggcc acgcccgggg tgagcaccac cacggtggga
     2941 tcgtctgtat tcggtggggc gatggcacgc agagcacgga gaagcgatgc cccgtagtga
     3001 tcaacgggtc tgatgacgtg actggcgaac ccgaccggga ggatccgggt catcgccacg
     3061 cggttctcca ggacgtagga gatgccgctc ggcactcgca ggttgtcttc gagcacccga
     3121 taggtgccct cgtcgtcacg gacgatgtcg atcccggcga cgacgcagcg ggagccccgg
     3181 ggagcgggga tgcc
//
LOCUS       DASTXO010000300         4833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1639320, whole genome shotgun sequence.
ACCESSION   DASTXO010000300 DASTXO010000000
VERSION     DASTXO010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1639320"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VFU96_06410"
     CDS             <1..181
                     /locus_tag="VFU96_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112934.1"
                     /translation="MAADVLMAAPNQGLGRGSKLLIDRTHQSLADLGDDRSTAAAQRA
                     VVEALRSPPGATDDS"
     gene            178..1506
                     /locus_tag="VFU96_06415"
     CDS             178..1506
                     /locus_tag="VFU96_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5112935.1"
                     /translation="MTEAETRYVLTPDGVHIAYQVTGGGDIDLVMMHGTVSHLEIAWE
                     DPQLRRLYDRLGSFARLIRFDRRGMGMSDGLRELPTVWQQVDDWRVVMEAVGVARPAL
                     FGTTDAGLLATLFAAEHPDRVRALVIFESAPRLMQSPGDSYGVDMSMMDRITDASQRI
                     DLDAHLEIVAPSRKDEPGFPAWFRRYNRSASSGIPAGAFFRDQADWDAVDRLPEVVAP
                     TLLLHRRENHVLPIRSSRAWADALPNGRLVELDGTGTAIYSDDVDPIADEIEAFLTGT
                     RPLPRQDRVLTTVLFTDIVDSTGRAATLGDHAWRELLARHHAVLRDHLARFGGREMHT
                     AGDGFLATFESPRHAIQCATAVRDAVDAIGLAIRAGVHTGEVELTEDDVQGIAVHIGA
                     RVSALAGPGQVFVSSTVRELVIGSGLEFEDRGSHELKGVPGEWRVFEVVG"
     gene            complement(1541..2179)
                     /locus_tag="VFU96_06420"
     CDS             complement(1541..2179)
                     /locus_tag="VFU96_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007454953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEU5112936.1"
                     /translation="MGKIRFNVSISLDGYMAGPDQTEENPLGVGGMELHNWLFDLEAW
                     RESHGESGGRVDASTPVVHELETGYGSVVMGRNMFGPIRGEWGADPWRGWWGDNPPYH
                     KPVFVLTHYGREPLEMDGGTTFHFVTDGFDSAMDQALTAADDADVLVAGGASVIRHYL
                     ANGVIDEFWLSLVPLLLGSGERPLDGIPSSTNVELVDVIAAPKATHLKYVVR"
     gene            2324..3022
                     /locus_tag="VFU96_06425"
     CDS             2324..3022
                     /locus_tag="VFU96_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014690302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HEU5112937.1"
                     /translation="MATARTTGLLYLGVAVTGMFGFLLIRPEIYVVGDAAATYANLVE
                     REMLARLGVAFEFLVVLTQALAAVWFYKLFRSVSATAAGALMAFGLINSTAVLASSAF
                     LVTALGVALDPALAPGGDAAATVQLMYELSGAFWGVGALFFGLWLIPMGHLVRASQWM
                     PRPLGWILVGGGVGYILSAFVVYLIPGTPDSVELALTLLATVGEFWMIGYLLVKGVRS
                     DVALHTTVPEQVPV"
     gene            3116..>4833
                     /locus_tag="VFU96_06430"
     CDS             3116..>4833
                     /locus_tag="VFU96_06430"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5112938.1"
                     /translation="MGMSTSDTPRFEVVAGDQGRVFVGRQRELALLLTGLGAASSGRG
                     RLFLIGGEPGIGKTRLADEFAQEAKSQDALVMWGRCWEQGGAPAYWPWTQSLRGLLTE
                     VDVETLRLAAAGKGGVLAQIFPELRDHLPDLPEPPSLSPEAARFQLFDALAGFLVRLS
                     QHRMLVFVLEDLHAADDPSLLLLQFLAGELANGSVLVLGTYRDVEVSRDHPLAPVLAE
                     LSRSQATTRIDLMGLSEPDVARFITSLEGQVPPEELVRSITRETEGNPLFIEEVVRLL
                     VAEDRLWEHVGDRRVPIPVGVREVIGRRLESLPESCHRMLTIGAVLGREFSVEMLQRI
                     SGHEAGEVLENLRDAVAAKLIHNHPDEPGRFRFAHALIRESLYEELMPADRIRLHREV
                     GQALETTYAPNPEPHIGELAYHFFEAAPGGDVRKAVEYAMTAGKRATDQLAFEEAVRL
                     LNLALRVLEDYPDEKQRCEALLRLGEAQARSGATPTAKETFLEAAEIATRLDLAEMSA
                     RAAMGYAGRFPWHRAATDRLVIPLLKQALEALGRDDSVLRATLLSRLAGALRDQPSLE
                     PRVSLAR"
BASE COUNT          803 a         1509 c         1589 g          932 t
ORIGIN      
        1 gatggccgcg gacgttctga tggccgcgcc gaaccagggg cttggccgcg ggagcaaatt
       61 gctgatcgac cgcactcacc aatccctcgc cgacctcggg gatgacaggt cgacggcggc
      121 cgcgcaacgg gcggtagtgg aggctctccg gtcgccaccg ggggcgacgg atgactcatg
      181 accgaggcgg aaacccgata cgtgttgacc cccgacggtg tgcacatcgc ctatcaggtc
      241 accggcgggg gcgacatcga cctcgtgatg atgcacggca cggtctcgca cctcgagatc
      301 gcctgggaag acccacagct ccgccggcta tacgatcgat tgggctcctt tgcccggctg
      361 atccggttcg accgcagggg catgggcatg tccgacggac tgcgcgagct gcccaccgtc
      421 tggcagcagg tcgacgattg gcgcgtcgtg atggaagcgg tgggcgtcgc ccgacctgca
      481 ttgttcggca cgacggacgc cggcctgctc gcgaccctgt tcgcggcgga gcatccggac
      541 cgagttcggg ctcttgtgat ctttgagtcc gctcccagac tcatgcagtc ccctggcgac
      601 tcgtacgggg tcgacatgtc gatgatggat cgcatcaccg acgcctcgca acgtatcgac
      661 ctcgacgcgc acttggagat cgtcgcgcct agtcgcaaag acgagccggg atttccggcc
      721 tggttccgcc gctacaaccg atccgcgtcg agtggcatcc cggcaggagc gtttttccgc
      781 gatcaggccg actgggacgc ggtcgaccgc ttgcctgagg tcgtcgcgcc cacccttctc
      841 ctccatcgcc gggagaacca cgttctccct attcggagct cgcgtgcctg ggcggatgcc
      901 ttgcccaacg gacggcttgt cgagctcgac ggcaccggca cggcaatcta ttcagacgac
      961 gtggacccca tcgccgacga gatcgaagcg ttcctcaccg gtacccggcc gctgccacgg
     1021 caggaccgcg ttctgacgac ggtgttgttc accgacatcg tggactcgac agggcgggcg
     1081 gcgactctcg gtgaccacgc ctggcgcgag ctgttggccc gccaccacgc tgtgctccgg
     1141 gaccatctgg cccggttcgg cggtcgcgag atgcacaccg caggcgacgg gttcctcgcg
     1201 acattcgaat cgccgcgaca cgcgattcag tgcgcgacgg cggtgcgcga tgccgtcgac
     1261 gccatcgggt tggcgatacg agctggggtg cacacgggcg aggtggagct gactgaagat
     1321 gacgtgcaag ggatagcggt gcacatcggt gcccgtgttt cggcgctcgc cggccccggg
     1381 caggtcttcg tctcgtcgac cgtccgcgag ctggtgatcg gctccgggct tgagttcgaa
     1441 gaccgtgggt cacacgagct gaagggtgtc cccggcgagt ggcgcgtctt cgaagtggtc
     1501 ggttagccct cgtagaccac aagcccttcc ggcggtgttc tcagcggacg acgtatttga
     1561 ggtgagtcgc ctttggggcc gcgatcacat cgaccagttc gacattggtg ctggagggga
     1621 ttccatccag cggacgctcg ccggagccga gcagaagggg gacgaggctg agccagaact
     1681 cgtcgatgac gccgttggcc agatagtggc ggatcaccga agcgccacct gcgaccagga
     1741 cgtcggcgtc gtctgcggcc gtcaacgcct ggtccatcgc agaatcgaag ccgtcggtga
     1801 cgaagtggaa tgtcgtgcca ccgtccatct ccagtggctc tcgtccgtag tgggtgagca
     1861 cgaagacagg tttgtggtac ggcgggttgt ccccccacca accccgccag gggtccgcac
     1921 cccattcgcc acgtatcggg ccgaacatgt tgcggcccat gaccacggag ccatacccgg
     1981 tctcgagctc atgcacgacc ggtgtgcttg cgtcgactcg cccgccggac tcaccgtggg
     2041 actcccgcca ggcctccaaa tcgaatagcc agttgtgcaa ttccatcccc ccgacaccga
     2101 gtgggttctc ttcggtctgg tcgggcccgg ccatgtagcc gtccagtgag atgctcacgt
     2161 tgaatcggat cttccccatc gatctcctgg tgctgaccga cgaagggtag acgggccgtc
     2221 caaatcttcc gaggttgaca ctcttacgct gtacgactac cttacaaggt acgacttacg
     2281 tagtaagaca agccaaagga ggagggagat tatgacatcg tcaatggcca cggctcgaac
     2341 cacggggttg ctctatctgg gagtggcggt caccgggatg ttcgggttcc tgttgatccg
     2401 acccgagatc tatgtggttg gtgacgccgc cgccacctat gccaacctcg ttgaacgcga
     2461 gatgctggcc cgactcggtg tcgccttcga gttcctggtc gtgctcaccc aggcgcttgc
     2521 ggcggtctgg ttctacaagc tgttccggtc ggtcagcgcc actgcggcgg gcgcactgat
     2581 ggcgttcggc ctgatcaaca gcaccgcggt cctcgcaagc agcgcttttc tcgtgaccgc
     2641 ccttggggtc gctctcgacc cggcgcttgc tccgggtggc gatgccgccg cgaccgtgca
     2701 gttgatgtat gagctgagcg gcgcgttctg gggcgtcggt gccttgttct tcgggctgtg
     2761 gctgatcccg atgggacacc tggttcgagc ttcgcaatgg atgccccggc cgctgggctg
     2821 gatcctggtg ggcgggggag tcggctacat cctgagtgct ttcgtcgtct atctcatccc
     2881 cgggacgccg gattcggtgg aactcgcact gaccttgctg gcgactgtcg gagagttctg
     2941 gatgatcggc tacttgttgg tcaagggtgt gcggagcgac gtcgcgctac acacgaccgt
     3001 gccggagcaa gttccggtgt gacagccgca cacacgcggt ggcgcggcag ggtgttccca
     3061 agacgacctg aagaaaggca caccggtagt ctgtcggcgg gacaggaggc gcggcatggg
     3121 gatgtccacc agcgacactc ctcgcttcga ggtggtcgca ggcgatcaag gtcgtgtgtt
     3181 tgtcgggcga cagcgagagc tcgcgcttct tctgaccgga ctgggcgcgg catcttcagg
     3241 tcgtggacgt ctcttcctga tcgggggcga gccggggatc ggtaagaccc gccttgcaga
     3301 tgagttcgca caggaggcga agtctcagga cgccttggtg atgtggggac gctgctggga
     3361 gcaaggtgga gccccggcgt attggccttg gacccagtcg cttcgtggtc tgctgactga
     3421 ggtggacgtc gagaccttgc gtctagccgc agcggggaaa ggcggcgtgc ttgcccagat
     3481 ttttcccgag ctccgagacc atctgcccga tcttccagaa cccccctctc tgtctcccga
     3541 agcggcgcga tttcagcttt tcgacgccct cgccggcttc ctcgtgaggc tttctcagca
     3601 tcgaatgctg gtattcgtcc tcgaggacct gcacgcggcc gatgaccctt cgctcctttt
     3661 actccagttc ctcgctgggg agttggcgaa tgggagtgtt ctggtgcttg gaacctatcg
     3721 ggatgtggag gtcagtaggg accatccgct cgcacctgtt ctcgccgagt tgtcccgcag
     3781 tcaggcaacg acgcgaatcg atctcatggg gctgagcgaa ccagatgtgg ctcgattcat
     3841 cacgtctttg gagggccagg tgcccccgga ggagctcgtc cggtcgataa cgcgcgagac
     3901 cgaaggcaat cccctcttca tcgaagaggt cgttcgactc ttggtggctg aggatcgtct
     3961 gtgggagcac gtgggagatc ggcgggttcc gatccccgtc ggggttcgtg aggtcatcgg
     4021 tcgccgcctg gaaagcctgc cggaatcgtg tcaccgaatg ctcacgatag gagccgtcct
     4081 cggccgcgag ttttccgtcg agatgctcca acgcatctcc gggcacgaag ctggagaagt
     4141 cctcgagaac ctacgcgacg ccgtcgcggc gaaattgatc cataaccatc cagacgagcc
     4201 gggccgattc cgattcgccc acgcccttat ccgtgaatcc ctctacgaag agctgatgcc
     4261 ggctgaccga atacgtcttc atcgtgaagt aggtcaggct ctggaaacga cctacgcccc
     4321 caaccccgaa ccgcatatcg gggagctcgc ctaccacttc ttcgaggccg cccctggagg
     4381 cgatgtccgg aaggcggtcg agtatgcaat gacggccgga aagcgcgcca ctgaccaact
     4441 cgccttcgaa gaggcggtcc gtctactcaa cctggcgttg cgcgtgctgg aggattaccc
     4501 ggacgagaag cagcgctgtg aggcgctgct ccggttgggt gaggcccagg cgcgatccgg
     4561 ggccacaccg acggctaagg agacatttct cgaggctgca gagatcgcaa ctcgactcga
     4621 cctggcggag atgtcggctc gggccgccat gggctacgcc ggcaggtttc catggcaccg
     4681 ggctgcaacg gaccgactcg ttatccccct gctgaagcag gctctcgagg ctcttgggcg
     4741 tgacgacagc gtgctcaggg cgacgctgct cagccgtctg gctggagcgt tgagggatca
     4801 gccatccctc gagccccggg tgtcgctagc tcg
//
LOCUS       DASTXO010000301         2446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1455675, whole genome shotgun sequence.
ACCESSION   DASTXO010000301 DASTXO010000000
VERSION     DASTXO010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455675"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VFU96_06435"
     CDS             <1..372
                     /locus_tag="VFU96_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5112939.1"
                     /translation="AMKELGMETSFADRYLNEGFSGGERKRNEVLQMAVLKPDLAIMD
                     ETDSGLDIDALRTVADGVNKLTGPERGFLIITHYQRLLDYVTPDRVHIFMDGRVLESG
                     GPELAGRLEQDGYDSFRTVLT"
     gene            375..1592
                     /locus_tag="VFU96_06440"
     CDS             375..1592
                     /locus_tag="VFU96_06440"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HEU5112940.1"
                     /translation="MTDLSHLKADFPVLGNGVHFLDSGASSQKPIPVLEAMDRFARTS
                     YANVHRGAYKLSVASTEAYEAARARVAGFIGAADPREVVFTRGTTTALNTVAFGWGLE
                     HLGPGDRILATEIEHHANLVPWQMVAKRTGATLDHVPMSDDFRLDLVAYEDKLGPEVK
                     IVAVTGMSNVLGILPPIRFISDLAHRHGARVVVDAAQLVPHTSVDVKALGADFVAFSS
                     HKMLGPTGIGVLWGKMAALEETEPFEGGGEMISDVTLHDATWAEIPHRFEAGTPPIIE
                     AVGLHAAIDYLESIGMDQVLAHDLELTGIALDRLSDVPGIQIQGPTSLEDRGGVVSMS
                     MDGVHAHDLATILDEGGVAVRSGHHCAKPLMRRLGVAATARASFYVYNTEEDVDALVG
                     GLRRAGELFGVGS"
     gene            1589..2038
                     /locus_tag="VFU96_06445"
     CDS             1589..2038
                     /locus_tag="VFU96_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEU5112941.1"
                     /translation="MSLEDLYREVILDHYRNPRNKGHLENPDAKAEGVNPLCGDELTI
                     ELNFEDERVTAVAIDGQGCSISQSSASMMTEAIKGKTRSEIEALTQQFRDMMSLDGET
                     EIELDPDRPGSVLGDLESLQGVRKYPVRIKCASLGWNVLLEALEVTG"
     gene            complement(2047..2244)
                     /gene="xseB"
                     /locus_tag="VFU96_06450"
     CDS             complement(2047..2244)
                     /gene="xseB"
                     /locus_tag="VFU96_06450"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU5112942.1"
                     /translation="MTDSQQTYREALEELRVIHARLREDEVDIDTLLADVQRASDLLA
                     FCRERLNSVGERLEEVLTDFE"
     gene            complement(2241..>2446)
                     /locus_tag="VFU96_06455"
     CDS             complement(2241..>2446)
                     /locus_tag="VFU96_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014507983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU5112943.1"
                     /translation="LQRHREHLVVLAEMFAAIGVEPTLRRGFALVTRPDGSVVRSAGE
                     LGAGDRVLVKLADGSVPVVVEAN"
BASE COUNT          463 a          797 c          739 g          447 t
ORIGIN      
        1 gccatgaaag agctgggaat ggagacctcc ttcgccgacc ggtacctcaa cgagggattc
       61 tccggcggtg agcgaaagcg aaacgaggtc ctgcagatgg ccgtgctcaa gcccgacctc
      121 gcaatcatgg atgagaccga ctccgggctg gacatcgacg ccctccgcac cgtggccgat
      181 ggcgtcaaca agctcaccgg ccctgaacgt gggttcctca tcatcaccca ctatcagcgt
      241 ctcctcgact acgtgacccc ggaccgggtt cacatcttca tggacggccg ggttctggag
      301 agtggtggtc ccgagctcgc cggccggctc gaacaagatg gatacgattc gttccggacg
      361 gtgttgacct agacgtgaca gatctctcgc atctgaaagc cgatttcccc gtcctcggca
      421 acggggtcca tttcctcgat tcaggtgcgt cgtcacagaa gccgattccc gtgttggagg
      481 cgatggaccg cttcgcgcgg acgtcgtatg ccaatgtgca ccgcggcgcc tacaagctct
      541 ccgtcgcatc tacagaggcc tacgaggcgg cacgggcccg tgttgccggc ttcatcggcg
      601 ccgccgaccc gagggaggtg gttttcacca gaggcacgac caccgccttg aacacggtcg
      661 ccttcggttg ggggttggaa cacctcggtc ctggcgaccg catcctcgcc accgagatcg
      721 agcatcacgc gaacctcgtc ccgtggcaga tggtcgccaa gcgaaccggc gccacgctcg
      781 accatgtgcc catgagcgat gatttccggc tggatctcgt cgcctatgag gacaagctag
      841 ggcccgaagt gaagatcgtg gcagtgaccg ggatgtcaaa tgtcctcggg atcctgcctc
      901 cgatccgttt catctccgat ctcgcccatc gtcatggcgc ccgggtcgtg gtggatgcgg
      961 ctcaactcgt cccgcacacg tccgtcgatg tcaaagccct cggggccgac ttcgttgcgt
     1021 tctcctcgca caagatgctc ggtcccaccg ggatcggggt cctctggggc aagatggccg
     1081 cactcgagga gaccgagccg ttcgaaggtg gcggcgagat gatctccgac gtgactctcc
     1141 atgatgcgac ctgggcggag atcccgcatc gcttcgaggc ggggacccct ccgatcatcg
     1201 aagccgtggg cctacacgct gcaatcgact acctcgaatc gatcgggatg gaccaggtgc
     1261 ttgcccacga cctggagctc accggtatcg cactcgatcg tctcagtgac gtccctggca
     1321 ttcagataca aggtcccacc agcctcgagg accgaggggg agtcgtctcg atgtcaatgg
     1381 atggagtcca tgcccacgat ctggccacga tcctcgatga aggcggtgtc gcggttcgct
     1441 ccgggcatca ttgcgccaag cccctgatgc gtcgtctcgg cgtggcggcg acggcccggg
     1501 ccagcttcta cgtctacaac accgaagagg acgtcgacgc cctggttggt ggccttcgcc
     1561 gggccggcga gttgttcgga gtcggatcgt gagcctcgag gacctctacc gcgaagtcat
     1621 cctcgaccac taccgcaacc cccggaacaa agggcatctg gagaacccgg acgccaaagc
     1681 cgagggggtc aacccccttt gtggtgatga gctcaccatc gagctcaact tcgaggacga
     1741 gcgcgtcacg gcggtggcga tcgacggaca gggttgctcg atcagccaat cgtcggcttc
     1801 gatgatgacc gaagcgatca aaggcaagac ccggtccgag atcgaggctc tcacccagca
     1861 gttccgggac atgatgtccc tggacggcga gaccgagatc gagctcgacc ctgatcgccc
     1921 cggctccgtg ctcggtgatc tcgagtcgct tcaaggcgtc cgcaagtatc cggttcgaat
     1981 caaatgcgcc agcctcggat ggaacgtcct gctggaggcg ttggaggtga ccgggtagcc
     2041 gtctcgctac tcgaagtcgg tgaggacctc ctcgagacgc tcaccgaccg agttgagacg
     2101 ctcgcggcag aaggcgagta gatccgatgc ccgttgcacg tcggcaagca gggtgtcgat
     2161 gtccacctcg tcttcccgaa gccgggcatg tatcaccctg agctcctcaa gcgcctcccg
     2221 ataggtctgt tgcgaatcgg tcaatttgcc tccactacca ccggcaccga gccgtcggcc
     2281 agtttcacca ggactcgatc accggcgccc aattcaccgg cactgcgcac caccgatcca
     2341 tcgggtctgg tgacgagggc aaaacctcgt ctcaaagtcg gctcaacacc gatggcggcg
     2401 aacatctcag ccaacaccac cagatgctcc cggtgtcgct gcagac
//
LOCUS       DASTXO010000302         9695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_386917, whole genome shotgun sequence.
ACCESSION   DASTXO010000302 DASTXO010000000
VERSION     DASTXO010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 9695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9695
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386917"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VFU96_06460"
     CDS             complement(<1..253)
                     /locus_tag="VFU96_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEU5112944.1"
                     /translation="MPTVRLDLAYDGSGFRGYAFQEGLRTIQGDLDRALQTVLGMPVE
                     TVVAGRTDAGVHARGQVISALVDHEVDLERLSRSLNGIVG"
     gene            complement(253..765)
                     /locus_tag="VFU96_06465"
     CDS             complement(253..765)
                     /locus_tag="VFU96_06465"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HEU5112945.1"
                     /translation="MFSRAGRAAILDRKPFTEAYFDGDSAADAAILVALVAAITYVGR
                     LLITGAIGAFSLPGLLESVIAGVVSWLILGFASWFAATRLFQSTGRPQTFIALQGLAV
                     LPLVLELFGRIGGGIGLIWYLVILGVGTREAANIDTRNAAVSVLIGFAIAAIFRTLLG
                     VPFVVFSALF"
     gene            complement(796..1728)
                     /gene="coaA"
                     /locus_tag="VFU96_06470"
     CDS             complement(796..1728)
                     /gene="coaA"
                     /locus_tag="VFU96_06470"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEU5112946.1"
                     /translation="MVSLEPLSEPNYLDFTSDEWAALRANTPLTLTESDVEKIRGINV
                     NLDIDMVEHSFLPLSRLLNLHVQGSHQLATVTDTFLGAPPRPRPFVIGVAGSVAVGKS
                     TISRVLQELLARWPQHPRVELVTTDGFLHPNAVLEDRGLMDRKGFPESYDRRALLEFV
                     ARAKGGAEHLEVPVYSHFAYDVLVGETRVITRPDILILEGLNVLQAVGVGAFVSDYFD
                     FSIFVDADPEAVRGWYIERFITLRDTAFTQPDAYFHRYAELDDEQATAVASDIWARIN
                     EVNLFENILPTRERASLILRKAPDHSIERVRLRR"
     gene            1784..2464
                     /locus_tag="VFU96_06475"
     CDS             1784..2464
                     /locus_tag="VFU96_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112947.1"
                     /translation="MGQFQGSLRLPGDSRPLPATVQLSEGRLQVASGEHVIGDWPVDA
                     IDISHVPEGIKVKAEGEVLLLELPDRDAFAQAAASQTQKPKRLSVPKRAAKTPAAVSP
                     KPAAAKAVKVKTAKSKTPKVRGESKLDAFLARAQDRYGAKLPNWVFGRWGIAAAVALV
                     AFCVIFAELVSNLLLIAGVIVLLVGGVTMLDSVIARRLLHHKITPIQVVLGGGGIFVV
                     GLIVGFAA"
     gene            complement(2484..2834)
                     /gene="rplQ"
                     /locus_tag="VFU96_06480"
     CDS             complement(2484..2834)
                     /gene="rplQ"
                     /locus_tag="VFU96_06480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU5112948.1"
                     /translation="MPRPRKGPRFGGSPSHDRKIMANLAIELFLHEKVTTTLPRAQAV
                     RPLAEKLITKARKGDLHAKRTVMRTIPNREAVVKLFDDVAPRYTDRQGGYTRITKLGP
                     RRGDGAEEAVIELV"
     gene            complement(2834..3775)
                     /locus_tag="VFU96_06485"
     CDS             complement(2834..3775)
                     /locus_tag="VFU96_06485"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016444112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU5112949.1"
                     /translation="MIVQRPQIEEEAISDTRSKIVVEPLEPGFGYTLGNTLRRTLLSR
                     IPGAAITSIQIEGVEHEFSTIPGVVEDVVDIILNIKRIVVRLEDTDTQTLYLSAKGAG
                     EVKAGDMKLPAGVEIVNPELHVATLSASGRLEMEMTVAPGVGYRTADGNKGSSSVIGV
                     IPVDSIFSPVRKVSYKVENTQVGQMTNFDRLVLDVETNGAIEPGEAVSSAGKTLRELM
                     GLFADMGEGQGLELGEVQPEFSGSPDLELPIEALDLSERPRNCLRRAQIKTVGELVER
                     TSDDLLNITNFGQKSLEEVTAKLDELGLSLADAGGNH"
     gene            complement(3788..4393)
                     /gene="rpsD"
                     /locus_tag="VFU96_06490"
     CDS             complement(3788..4393)
                     /gene="rpsD"
                     /locus_tag="VFU96_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017976781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU5112950.1"
                     /translation="MARYTGPRHKACRRARTPLCQSKRCPVDRRPYPPGQHGRGRIRE
                     SDYLIQLREKQKLRAMYGVLERQFRLYYKEAARRPGITGDNLLQLLEQRLDNVVWRAG
                     LAATRPQARQLVNHGHFRVNGRKVDIPSYQVRPGDVVTLKDKSRDAIVIQHAVDTQGR
                     EAPAWLHVDLGERKVDVTDHPRRDQIDTAINEQLIVELYSK"
     gene            complement(4397..4801)
                     /gene="rpsK"
                     /locus_tag="VFU96_06495"
     CDS             complement(4397..4801)
                     /gene="rpsK"
                     /locus_tag="VFU96_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU5112951.1"
                     /translation="MQRVAKQQGAKRVRRRERRNISHGQAHIKASFNNTIISITDQTG
                     ATVAWTSGGSVGFKGSRKSTPYAAQVAAEEAARRAGEHGIRKLDVIVSGTGSGRDTAV
                     RTLQNMGMEVTWIRDVTPIPHNGCRPKKRRRG"
     gene            complement(4806..5183)
                     /gene="rpsM"
                     /locus_tag="VFU96_06500"
     CDS             complement(4806..5183)
                     /gene="rpsM"
                     /locus_tag="VFU96_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018224910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU5112952.1"
                     /translation="MARIAGVDLPRDKRLEIGLTYIYGIGLTRAQKVCSETGVDPDTR
                     VRQLTDDEVTRIRRYIEANFEVEGDLRREVQQNIKRKIEIGSYQGIRHRRGLPVRGQR
                     THTNARTRKGRRVAIAGKKKVTK"
     gene            complement(5189..5302)
                     /gene="rpmJ"
                     /locus_tag="VFU96_06505"
     CDS             complement(5189..5302)
                     /gene="rpmJ"
                     /locus_tag="VFU96_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006042225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEU5112953.1"
                     /translation="MKVRPSTKKMCEKCRIIRRRGRVWVICSNPRHKQRQG"
     gene            complement(5444..6238)
                     /gene="map"
                     /locus_tag="VFU96_06510"
     CDS             complement(5444..6238)
                     /gene="map"
                     /locus_tag="VFU96_06510"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEU5112954.1"
                     /translation="MITIKSRREFAKMARAGAAVAAIHEAVREAAVPGVSLLALEEIS
                     ARILEERGCTSSFLGYHGTYPATLCLSPNDVIVHGIPSEKRLEEGDILSVDAGAIYEG
                     FHGDAAFTVAIGTVSSDARRLIDVTEEALWVGIHEVRKGGHLGDIGAVVADIGKSNGF
                     GVVEEYVGHGIGRQMHEEPQVPNYGRRGQGLRLRTGMALCIEPMFNLGSRHSTVDDDG
                     WTVRTADGTLSAHWEHTVCLTPDGTQVFTAGEEPVPVEFEQSAAHG"
     gene            complement(6235..6780)
                     /locus_tag="VFU96_06515"
     CDS             complement(6235..6780)
                     /locus_tag="VFU96_06515"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEU5112955.1"
                     /translation="MRILFIGPPGAGKGTQAARVAERLGIPHISTGDMFRHHVSTGTE
                     LGRLVDAIMKAGEYVPDEITVEMLRERLDEPDAATGYILDGFPRTAGQVAALDGLIGA
                     DGLDTVVVFDVDEDALVERLLGRGRADDTEDTIRTRFKVYQEQTTPLLDEYRNRGIVV
                     TIDGLGDIEDVTERILQVLAP"
     gene            complement(6777..8090)
                     /gene="secY"
                     /locus_tag="VFU96_06520"
     CDS             complement(6777..8090)
                     /gene="secY"
                     /locus_tag="VFU96_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014674383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEU5112956.1"
                     /translation="MLSIYRNMFKIPDLRGKILFTLAMFAVYRLGATIPVPGIDLQAV
                     ELFQSQQQAGGIYGLLNLFSGGALEAFSVFALGIVPYITASIIMQLLTVVIPRLQALQ
                     EEGESGRKVITQWTRYLTVILALIQSTGFTYLFHVGRFTGGIDLIPNYTPGRVILIVL
                     TMTAGTAMVMWLGELITQRGVGNGMSLIIFVSILSQFPFVFGQIWSQTVGAAQAFRFG
                     VIVAIFLAMIVAIIYVDQAQRRIPIQFAKRVRGRRVMGGQSTYIPLKVNTAGVIPVIF
                     ATSVMLIPSLIITAIPSDAAWVQSIYRWIDGNLGGGTGGVSWWYILILFALIIFFTYF
                     YTAIQFDPEKQAEMIQRQGGFVPGVRPGRQTARHLAFILNRITLPGSIFLALIAILPA
                     IAGAVWGINIAFSGVSILIVVGVALETMKQIESQLTMRNYEGILT"
     gene            complement(8132..8569)
                     /gene="rplO"
                     /locus_tag="VFU96_06525"
     CDS             complement(8132..8569)
                     /gene="rplO"
                     /locus_tag="VFU96_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004888697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEU5112957.1"
                     /translation="MKLHHLQPAPGSKKERIRVGRGEGGRRGKTAGRGTKGLKARNTL
                     RPGFEGGQTPLSMRIPKLPGFTNPNKEEFAIVNLAALDVFDKGSEVTPEELRNRGLIR
                     HRGRVKVLAQGELDKALTVKAHAFSAAAKAKIEKAGGTAEVIS"
     gene            complement(8595..8780)
                     /gene="rpmD"
                     /locus_tag="VFU96_06530"
     CDS             complement(8595..8780)
                     /gene="rpmD"
                     /locus_tag="VFU96_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006828946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEU5112958.1"
                     /translation="MADKIVRVTLRRSVIGEKPKTRATVESLGLRKLHQSREHVDSPS
                     LRGMLHKVRHLVEVEEG"
     gene            complement(8788..9336)
                     /gene="rpsE"
                     /locus_tag="VFU96_06535"
     CDS             complement(8788..9336)
                     /gene="rpsE"
                     /locus_tag="VFU96_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEU5112959.1"
                     /translation="MAQDQQTQFDERVIQINRVAKVVKGGRRFSFTALVVVGDGAGKV
                     GIGYGKAKEVPAAIQKGMETARRAMVEIPMAGQTVVHQATGEHGASRVLIKPAAPGTG
                     VIAGGAVRQILEAAGVKDALAKSLGSPTHLNVAKAAMNALTGQRRPDEVARLRGKQAE
                     DIVPPGLLAAYRSTESMRAHTK"
     gene            complement(9339..9692)
                     /gene="rplR"
                     /locus_tag="VFU96_06540"
     CDS             complement(9339..9692)
                     /gene="rplR"
                     /locus_tag="VFU96_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU5112960.1"
                     /translation="MRGSREDARSRRHTRVRKRVHGTGERPRLAVYRSNKYIYAQVID
                     DVEGRTLAAASSQESNLRSDNLNLAAAAKVGEAIASRAKDAGVKTVVFDRGGYKFHGK
                     VKALADAAREAGLEF"
BASE COUNT         1715 a         3069 c         3056 g         1855 t
ORIGIN      
        1 gcccgacgat tccattgagg gagcgactga gccgctccag gtcgacttcg tggtcgacga
       61 gggctgaaat cacctgtcct ctcgcatgca caccggcatc ggtgcgcccg gctacgacgg
      121 tctccaccgg cattccaaga acagtttgca acgccctgtc gagatcaccc tgaatggttc
      181 tcagcccttc ctgaaaggca tagcccctga aacctgagcc gtcgtaggcc aggtcgagcc
      241 gaaccgtggg catcagaaca gggcggagaa gacgacgaag gggacgccga gcagggtgcg
      301 gaagatggcg gcgatcgcaa acccgatcaa taccgacact gcggcgttcc tggtgtcgat
      361 gttggctgcc tcacgcgtcc cgactccgag gatcaccaga taccagatca ggccgatgcc
      421 cccaccgatc ctgccgaaca actccaaaac cagcggtagc acggcgagac cctggagcgc
      481 gatgaatgtc tgcggccggc ctgtcgactg gaacagtcgg gttgcagcga accaggaggc
      541 gaaaccgagg atcaaccacg acacgacacc ggcgatcacg gattcgagca ggccgggcag
      601 tgaaaatgcc ccgatcgcgc cggtgatcaa gagcctgccg acgtaggtga tcgcggcaac
      661 cagcgccacc aggatggcgg catcggcggc cgaatcaccg tcgaagtagg cctcggtgaa
      721 cggtttgcga tcgaggatgg cggcccgacc cgcccgagag aacattctgc tcacagtcgg
      781 gcgagggtaa ccgggtcagc gacggagccg gaccctctcg atcgagtgat ccggggcctt
      841 cctcaggatg agactcgccc tttctcgggt ggggaggatg ttctcgaaga ggttgacctc
      901 gttgatccgg gcccagatat cggaggccac cgcggtcgcc tgctcatcat cgagctcggc
      961 gtaacgatgg aagtaggcat cgggctgggt gaaggcggtg tcacgaagcg tgatgaatcg
     1021 ttcgatgtac cagccccgca cggcctccgg gtccgcatcg acgaagatcg aaaagtcgaa
     1081 atagtcacta acgaaagcgc cgacgcctac tgcctggaga acgttcaatc cctcgaggat
     1141 caagatgtcg ggccgggtga tcacccgggt ctcccccacg agcacgtcgt acgcgaaatg
     1201 tgagtacacc ggcacctcga gatgctcggc tcctcccttg gcccgggcga cgaactcgag
     1261 gagggcgcgc cggtcgtagc tctcggggaa gccttttcgg tccatcagcc cccggtcctc
     1321 caacacggcg ttggggtgga ggaacccatc cgtggtcacc agctcgacac ggggatgttg
     1381 gggccatctg gcgagaagct cttgcagcac gcgggagatc gtcgacttcc ccaccgccac
     1441 cgaaccggcc accccgatga cgaatggtct cggacgtggg ggtgcgccca gaaaggtgtc
     1501 ggtgacggtc gccagttggt ggcttccctg gacgtgcaga ttcaggagcc gagagagggg
     1561 gagaaagctg tgctcgacca tgtcgatgtc gagattgacg ttgattcccc tgatcttctc
     1621 gacgtcagac tccgtgagtg tcaggggagt gttggcccgc agcgcggccc attcgtcgct
     1681 ggtgaaatcg agatagttgg gctcgctcaa cggctcaagg ctaaccatcg agatttctca
     1741 tttttcgcta agccccgacc cgaaacggcc gataaagccg tcgatgggac agttccaggg
     1801 ctctctgcgg ttgcccggcg acagccgtcc gctccccgcg accgtccagc taagtgaagg
     1861 ccgtctccag gttgcatcgg gcgaacacgt catcggcgac tggccggtcg acgcaatcga
     1921 catctcccat gtccccgagg ggatcaaggt caaggcagag ggggaggtcc tcctcctcga
     1981 gctccccgat cgtgacgcct ttgctcaggc cgcagcttcc cagacccaga agcccaagcg
     2041 tctctccgtg cccaagcgag ctgcgaagac gcctgccgcc gtgtccccga agccggcagc
     2101 tgccaaggcc gtgaaggtca agaccgccaa gtcgaagacc cccaaggtgc ggggggagtc
     2161 gaagctcgat gcctttctag ccagagccca agatcgatac ggagccaagc tgccgaactg
     2221 ggtcttcggt cgctggggaa tcgcagccgc cgttgcgttg gtcgctttct gcgtgatctt
     2281 cgccgagctc gtttccaacc tgctcctgat cgcaggtgtc attgtcttgc tagtcggcgg
     2341 ggtgaccatg ctggacagcg tgatcgcccg tcgtctcctc catcacaaga tcacgccgat
     2401 ccaggtggtc ctcggtggtg gtggcatctt cgtggtcgga ctgatcgtcg gcttcgccgc
     2461 ctgaagttct cccgactctc ttctcagacg agttcgatga ccgcttcctc ggcgccgtct
     2521 ccacgacgtg ggccgagttt ggtgattcgg gtgtagccgc cctgacggtc ggtgtaccga
     2581 ggggccacgt cgtcgaagag cttgacgacg gcttcccggt tggggattgt ccgcatcacg
     2641 gttcgcttgg cgtgtaggtc acccttgcgt gccttggtga tcagcttctc ggccagtggc
     2701 cgcaccgctt gggcgcgggg cagggtggtc gtcaccttct cgtgcaggaa gagctcgatg
     2761 gcgaggttgg ccatgatctt ccgatcgtgc gagggcgagc caccgaagcg ggggcccttg
     2821 cgggggcgtg gcatcagtgg tttccgccgg cgtcggccag gctcagtccg agctcgtcga
     2881 gcttggccgt cacctcttcg aggctcttct gcccaaagtt ggtgatgttg aggagatcgt
     2941 ccgacgtccg ctccacgagt tccccgaccg tcttgatctg ggcacggcga aggcagttgc
     3001 ggggacgctc tgagagatcg agtgcctcga tcgggagctc gaggtccggg gacccggaga
     3061 actcgggctg gacctcgccc agctcgagac cctggccctc gcccatgtcg gcgaacagcc
     3121 ccatgagctc acggagggtc ttgcccgctg aagaaaccgc ctcgccgggc tctatggctc
     3181 cattggtctc cacatcgagc acgagccgat cgaagttcgt catctggccc acctgggtgt
     3241 tctccacctt gtaggacacc ttgcgcacgg gcgagaagat cgagtcgacc gggataacgc
     3301 caatgaccga gctggagccc ttgttgccgt cggcggtgcg ataccccact ccgggggcca
     3361 cggtcatctc catctccagc cggccgctgg cgctcagggt ggcgacgtgc agttcgggat
     3421 tcacgatctc gactccggca gggagcttca tatcgcctgc cttgacctca ccggcaccct
     3481 tggccgacag atagagggtc tgggtgtcgg tgtcctcgag acgaaccacg atccgcttga
     3541 tgttgaggat gatgtcgacc acgtcctcga ccacgccggg gatggtcgag aactcatgct
     3601 cgactccttc gatctggatc gaggtgatgg cggcaccggg gatgcgcgac aggagcgtgc
     3661 gacgcaaggt gttgccgagg gtgtatccga atccaggttc gagaggctcg acgacgatct
     3721 tcgacctggt gtctgagatc gcttcctctt cgatctgggg gcgctgaaca atcaacaagg
     3781 cattacctca cttcgagtag agctcgacga tcaactgctc gttgatggct gtatcgatct
     3841 ggtccctgcg cgggtgatcg gtcacatcca ccttccgctc gccgaggtcg acatggagcc
     3901 aggccggagc ctcgcgtccc tgcgtgtcca cggcgtgctg gataacgatt gcgtccctgc
     3961 tcttgtcttt cagggtgact acgtcaccgg gacgaacctg ataggacggg atgtccacct
     4021 tccttccatt gacccggaag tgcccgtgat tgacgagctg gcgggcctgg ggtcgggtgg
     4081 cggcgaggcc ggctcgccaa acgacattgt ccagcctctg ctcgaggagc tgaaggaggt
     4141 tgtcacccgt gatcccgggc cgacgggcgg cctccttgta gtagaggcga aactgccgct
     4201 cgaggactcc atacatggca cgcagcttct gcttctcgcg aagctggatg agatagtcac
     4261 tctcacggat gcggccacgt ccgtgctggc ccggagggta agggcgacga tcgaccgggc
     4321 accgcttcga ttggcagagc ggagtgcggg cacgacggca tgccttgtga cgagggccgg
     4381 tgtaacgagc catgactcag cccctccgtc tcttcttcgg acggcagccg ttgtgcggga
     4441 tcggtgtgac gtcacgtatc caggtgactt ccatccccat gttctgcagg gtcctcaccg
     4501 cggtgtcacg acccgagccg gttccgctca cgatgacgtc gagcttgcgg ataccgtgct
     4561 cgccggcccg gcgagcagcc tcctcagcgg cgacctgcgc cgcgtacgga gtcgacttgc
     4621 gcgacccttt gaaaccaacc gacccgccgg acgtccaggc gacggtcgca ccggtctgat
     4681 cggtgatcga aatgatcgtg ttgttgaaac tggccttgat gtgggcctgg ccgtgggaga
     4741 tgtttcttcg ctcccgacga cgcacacgct tggcgccttg ttgctttgcc actctctgca
     4801 aactcctact tggtgacctt cttcttgccg gcgatggcca cacgccgccc cttgcgggtg
     4861 cgggcgttgg tgtgcgtgcg ctgtccgcgc acgggcaggc cgcgacggtg gcggatgccc
     4921 tggtagctgc cgatctcgat ctttcgcttg atgttctgct ggacttcccg tcgcaggtcg
     4981 ccttcgactt cgaagttcgc ctcgatgtaa cggcggatcc gggtcacctc gtcatcggtt
     5041 agctgccgga ccctggtatc ggggtcgacg ccggtctcgg agcacacctt ctgggcgcgg
     5101 gtcagcccga tcccgtagat gtaagtcagc ccgatctcga gcctcttgtc cctgggaagg
     5161 tcaactcctg cgatacgtgc cactcagatc atccttgccg ttgcttgtga cggggattgc
     5221 tgcagatcac ccagacgcgg ccacgacgtc tgatgatccg acatttctca cacatcttct
     5281 tggtggaagg ccttaccttc atcttctgct ccaaaactcg aataggcccg tcgcagggga
     5341 caacctgaac ccagaatctg attcgggggt aggggacgat ttcgtcgtcc tggcctgaat
     5401 gcgcgttgaa cgacgcgaaa aagcgccgca acaactcgca atgttacccg tgggctgcac
     5461 tttgctcaaa ttccaccggg acaggctcct cgccggccgt gaacacctgg gttccgtctg
     5521 gcgtgaggca aaccgtgtgc tcccagtgcg ccgaaagcgt gccatcggca gtgcggaccg
     5581 tccacccgtc atcatcgacc gtgctgtgtc tggaaccgag gttgaacatg ggctcgatgc
     5641 agagcgccat gccagtgcgc aggcggagac cctggccgcg ccgcccatag ttggggacct
     5701 gcggctcctc gtgcatctgc cttccgatgc cgtgacccac gtactcctcg acaaccccga
     5761 acccgttgct cttcccgata tcggcgacga cagccccgat gtcaccgaga tgaccgccct
     5821 tccgcacctc gtggattccc acccacagcg cctcctcggt cacatcgatg agtcggcgag
     5881 cgtcggaact gactgtgccg atcgcaacgg tgaaggccgc gtcgccatga aagccctcgt
     5941 agatcgctcc ggcatccacc gagaggatgt ccccctcctc gagacgcttc tccgagggga
     6001 tcccgtggac gatcacgtcg ttgggtgaga ggcagagggt ggccggatac gtcccgtgat
     6061 aaccgagaaa cgatgacgtg cagccgcgct cctcgaggat ccgggccgag atctcctcca
     6121 gtgccagcag ggagacgcca gggacagcgg cttctctcac cgcctcgtgg attgcggcta
     6181 cggcggcgcc ggccctggcc atcttggcga actcacggcg tgacttgatc gtgatcatgg
     6241 tgccagcacc tggaggatgc gctcggtgac gtcttcgatg tcacccaacc catcgatggt
     6301 gacgacgatg ccccgatttc gatattcgtc gagaaggggg gtggtctgct cctggtagac
     6361 cttgaatcgg gtgcggatgg tgtcttcggt gtcgtctgct cgaccccggc ccaagaggcg
     6421 ctccacgagc gcgtcctcgt cgacatcgaa gacgacgacc gtatcgagcc catcggcccc
     6481 gatcagccca tcgagcgcag cgacctggcc cgcggtgcgg gggaatccgt cgagaatgta
     6541 tcccgtcgcg gcatcgggct cgtccagccg ctcacgaagc atctcgacgg tgatctcgtc
     6601 gggcacgtat tcgccggcct tcatgatcgc atcgaccagc cggccgagct cggttcccgt
     6661 cgatacgtga tgccggaaca tgtctccggt cgagatgtgg gggatgccga gccgctccgc
     6721 cacccgggcc gcctgggtgc ccttgccggc gccaggcggt ccgatgaaca gaatcctcac
     6781 gtgaggatcc cctcatagtt gcgcatcgtc agctgggatt cgatctgctt catcgtctcg
     6841 agcgcaactc cgaccacgat caggatcgag accccggaga aggcgatgtt gatcccccac
     6901 accgccccgg cgatggcggg gaggattgcg atcagcgcca ggaagatgga gccgggcagg
     6961 gtgatccggt tgagtatgaa cgccagatgc cgcgcggttt gccgtcccgg gcgcaccccg
     7021 ggcacgaacc cgccttgacg ctggatcatc tcggcctgct tctcgggatc gaactggatg
     7081 gcggtgtaga agtaagtgaa gaagatgatc agcgcgaaca ggatcaggat gtaccaccag
     7141 ctgacgccgc ccgtcccccc gccgaggttt ccgtcgatcc accggtagat ggattgcacc
     7201 caggccgcat ccgacggtat tgcggtgatg atcagtgagg ggatgagcat caccgaggtg
     7261 gcgaagatga ccgggatcac ccctgcagtg ttgaccttga gcgggatgta ggtcgactgg
     7321 cctcccatta cccggcgtcc gcggactcgt ttggcaaact ggatcgggat ccgtctctgg
     7381 gcctgatcca cgtagatgat ggcgacgatc atcgccagga agatggccac gatcacaccg
     7441 aaccggaacg cctgggccgc accgacggtc tggctccaga tctggccgaa cacgaacggg
     7501 aactggctca ggatggagac gaagatgatc agcgacatgc cgttgcccac tccgcgctgg
     7561 gtgatcagct caccgagcca catcaccatg gcggtaccgg cagtcatcgt cagcacgatg
     7621 aggatgacac ggccgggggt gtaattgggg atgaggtcga ttccgccggt gaagcggccg
     7681 acgtggaaca agtaggtgaa gccggtcgac tggatcaatg ccagaatcac cgtcaggtac
     7741 ctggtccact gggtgatcac cttccgcccc gactcaccct cctcctggag ggcttggagc
     7801 cggggaatga ccacggtcag caactgcatg atgatcgagg cggtgatgta ggggacgatg
     7861 ccaagggcaa acaccgaaaa ggcctcgagg gcgccacccg agaacaggtt gagcaaccca
     7921 tagatgccgc cggcttgttg ctgtgactgg aacagctcca ccgcctggag gtcgatcccg
     7981 ggcacgggga tggtggcacc tagccggtag accgcgaaca tggccagggt gaagaggatc
     8041 ttcccccgga ggtccgggat cttgaacatg ttgcggtaaa tgctgagcat tgcgtttgtt
     8101 gatcctgcgt cttacgcgtt gtgggacaaa gtcatgaaat gacttcggcg gtgccgccgg
     8161 ccttttcgat cttggccttg gcggccgcac tgaaggcatg agccttcaca gtcagggcct
     8221 tgtccagctc accctgagcc agcaccttga cccgaccccg atgccgaatg aggccgcggt
     8281 tgcgaagctc ctcgggagtc acctccgagc ctttgtcgaa aacgtccagc gcggccagat
     8341 tcacgatcgc gaactcttcc ttgttggggt tggtgaaccc gggaagcttg ggaattcgca
     8401 tcgagagcgg ggtctgcccg ccctcgaaac cgggacggag cgtgttacgg gccttgaggc
     8461 ccttggtgcc ccggcccgcg gtcttgccac gtcggccacc ctcaccccgg cccacccgga
     8521 tcctctcctt cttggagcca ggtgccggct gcagatgatg cagcttcagt tgttcctttt
     8581 tttcttcggc catctcagcc ttcctcgacc tcgacgagat gccgcacctt gtgcagcatc
     8641 ccgcgcaggg atggggaatc gacgtgctcc cgggactgat ggagcttgcg caatcccagc
     8701 gactcgacgg tggcccttgt cttgggcttc tcgccgatca cggatctcct caatgtcact
     8761 ctcacgatct tgtcagccat cgaatcctca cttggtgtgg gcccgcatcg actctgtcga
     8821 gcggtaggcg gcgagcaggc cgggagggac gatgtcctcc gcctgcttgc cccgaagacg
     8881 ggcaacctca tcaggtcgac gctgcccggt caacgcattc atcgcggcct tggccacgtt
     8941 gaggtgggtg ggagagccca gtgacttggc cagggcgtcc ttgactccgg ctgcctccag
     9001 gatctgacgc acggcacccc cggcgatgac accggtgcca ggcgccgccg gcttgatcag
     9061 gacccgtgag gcaccgtgtt caccggtcgc ctggtggacc acggtttgac ccgccatcgg
     9121 gatctcgacc atggcgcgcc gtgccgtctc catacccttc tgaatcgctg ccggcacttc
     9181 cttggccttg ccgtagccga tgccaacctt gcccgccccg tcacccacca ccacgagggc
     9241 ggtgaagctg aaccgccgac cacccttgac cactttggcc acgcggttga tctgaatcac
     9301 acgctcgtcg aactgggtct gctgatcttg ggccatgcct aaaactccaa tccggcttcg
     9361 cgtgctgcat cggcaagggc cttgaccttg ccgtggaact tgtacccgcc ccggtcgaag
     9421 acgaccgtct taacccccgc atccttggcc cggctcgcga tcgcctcacc caccttggcc
     9481 gcggcggcca ggttgaggtt gtccgatcgc aggttcgact cctgtgagga agcggcagcc
     9541 agagtgcggc cctcgacatc gtcgatgacc tgggcgtaga tgtatttgtt gctccggtag
     9601 acggcgagac gagggcgctc ccccgtgccg tggacgcgct tgcgcacccg ggtgtgacgc
     9661 cggctccggg cgtcttctcg tgagcctcgc atcac
//
LOCUS       DASTXO010000303         6112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_865987, whole genome shotgun sequence.
ACCESSION   DASTXO010000303 DASTXO010000000
VERSION     DASTXO010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6112
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865987"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..681)
                     /locus_tag="VFU96_06545"
     CDS             complement(82..681)
                     /locus_tag="VFU96_06545"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5112961.1"
                     /translation="MIRPVEPGDLDALYDVCLRTGDAGEDASHRFADPKLLGEVYVGP
                     YLMMPSGIGFTAVERGVPSGYVLAAVDTRQFEAECEAEWWPPLRTRYPRPVVADSSTD
                     DAEVITLIHDPESPPDSIVATYPAHLHIDLLPVLQGKGLGRAMMSRIFEDLRVRGVAG
                     VHLGVDARNLRAVGFYEHLGFEHLDDTDDVVMGIRLDED"
     gene            781..1515
                     /locus_tag="VFU96_06550"
     CDS             781..1515
                     /locus_tag="VFU96_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU5112962.1"
                     /translation="MTVSSPDGKTQEAVRQLRHLVGTLEAGSRIPSERDLAAHWGVAR
                     MTARKAIETLTAEGWLDRRHGSGTYVAQMPYAKTLGLSSFTADMERRGLIPSTRVIDF
                     DRVPADSATAARLDIDEGEEVVRFTRLRLADDEPIAVETTWIPAHLVPGLGKDDLSGS
                     LFETLSQKFGITLGQASSTIDPALADPTTAAELDIEDHEPCLRIQMGYSDHRRRTVMA
                     ATCLYRGDRYQIHVVLTPAAFNSAPR"
     gene            1512..2246
                     /locus_tag="VFU96_06555"
     CDS             1512..2246
                     /locus_tag="VFU96_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU5112963.1"
                     /translation="MSAGERYLAGAGRILEQIAADEWPSIRIAAETVADSIAGDGLVH
                     VFGTGHSHMLAEEMFYRAGGLAAINPILIEGLMLHAGAEKSTHLERQHGLAGQIFSDL
                     PLKPGDTFLVASNSGGNAVCEELAQLAGEAGAAVIAIVSSRHAEHSAAERNGSRLADL
                     ADIVIDNHGDIGDAGVDIDGIARRVGPTSTVAGAAIVNAIVAEAVEILLERGVEVDVF
                     TSSNLPEGDAANAELVARYRDRVRAL"
     gene            2243..3838
                     /locus_tag="VFU96_06560"
     CDS             2243..3838
                     /locus_tag="VFU96_06560"
                     /inference="COORDINATES: protein motif:HMM:NF019193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HEU5112964.1"
                     /translation="MSHTVRWLSVPPEIDREHLDGMARQCGLTVADRADTNLTVAVTL
                     SADPDLTYDSAHRRISDETYILRLGEEGHAGLDVTGSRSLRWGLVDLAGKAGGEDWES
                     GERRQSPGFGIRGIIEGFYGPPWEDQARLDMIDFASRHRFNTFFYSPKDDPYLRRDWR
                     TPFEGKSLRRLEEVIERCRDRDIDSMIGVSPGLSMRYSDDKDNDLLAEKISSLIDLGA
                     TSIALLFDDIPDRLQHDADLSTFPDLAAAHAQTANRVFDGIAGPFVVCPTIYWGAGDE
                     EYISRLGDQVDPRIDLFWTGRAVCSPAITVTEAAHFARVNRRPPLYWDNYPVNDASMA
                     NEMHIGPFQNRDGALDRFCRGLMANAMEYPEASKIGLATIADYLWDPVGYDPETSWQR
                     AIVEIAGVTDAPALSLFADTVRASCLSDPDPLGLTAALQAYAFQIESGHPRAAATGLA
                     AYGLELTGAADHLLGRDMENRVLQAELAPWLAKFRSGAEAVTAIAARRGEGDLTESLK
                     HLREDPRRVFGDVLEMTLADLIK"
     gene            3853..5163
                     /locus_tag="VFU96_06565"
     CDS             3853..5163
                     /locus_tag="VFU96_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU5112965.1"
                     /translation="MKQRYGPLALLLALGLVVGACAEGATDDASDTTSTGETTAETTG
                     SEEEVVLRIAMGSPGEEQIGVWEQAKAAFEEENPNVSVELNFQDDDLYQTIGLLNLLN
                     GGEAPDIYFEWAGARLQSRYDEGFVADITEAVDGELGSYFDEGAFNGMVIDDKNVMVP
                     ANADVTTVFWYNTEIFDAVGVSVPTTWEELIDVSNSIKEAGIIPIAIGNKDLWPVGNW
                     TSHIVSRVIGEEAYDQMMRQEIPLDSPEVIEALGYVQQLADAGIVNESANAIDDNEGA
                     QLFFQGQAAMHPIGSWLVSWALEEAPDLSFDYFNLPEVPGAGTQDSVIGVSTGFVINE
                     NSEHKDVAIDFMTLFSSPEFTSLMVDAGGTPIATGAFDRDDIDPRVTAMMELLQGASA
                     LVAPPDTGYDLEVADALYAAEAEVLGGVATPEEAAAAAQAKLDQ"
     gene            5193..6074
                     /locus_tag="VFU96_06570"
     CDS             5193..6074
                     /locus_tag="VFU96_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU5112966.1"
                     /translation="MRPQRRITPYLLVAPALALFTFAVLGPIVATGVFSFFEWDGFGP
                     FDPVGFANYLRAAADPLFRASFVHVAIYIVLTIFLEVFVGLLLAGLVTARPKGTGLFR
                     VAFFIPVLLPMVVVAVLWDFVYNPDFGLLNSTLIAFGLEDFTQIWLGDPRFALTAVSV
                     VSGWVFAGFYMAIFYAGFRGIPGDVLEAARIDGASEIQTFWRVKVPMIRHVIEIALLL
                     CITGGFQAFDLFYVLTNGGPFNATEIPTTYLVRVVFRNQEVGYGSAMAVVMTLVVLII
                     GGLYVRLRRRTAVETVS"
BASE COUNT         1114 a         1969 c         1924 g         1105 t
ORIGIN      
        1 cattctctgt cggcacttgc ccgctcatcg cgtcacattc cgagtgtatt cgacgtggat
       61 cccgtgcccg ggcctcggac atcaatcctc atcgagtcgg atgcccatca cgacgtcgtc
      121 cgtgtcgtcg aggtgctcga acccgaggtg ttcgtagaac ccgacggcac gcaggttgcg
      181 ggcgtcgact ccgagatgga ccccggccac tcccctgacc cgaagatcct cgaagatgcg
      241 gctcatcatc gctctcccca aacccttccc ctgaagcacc ggaagcagat cgatatggag
      301 atgagccgga tacgtcgcca cgattgagtc gggcgggctc tctgggtcat ggatgagcgt
      361 gatgacctct gcgtcatccg tcgacgaatc tgccaccact ggacggggat agcgagtccg
      421 caacggcggc caccactctg cctcacattc agcctcgaat tgtcgggtgt cgaccgcggc
      481 caggacgtag cccgatggaa cgcccctctc gacagcggtg aacccgatcc cactcggcat
      541 catcagataa ggcccgacat agacctcgcc caggagcttt gggtcggcga accggtggga
      601 cgcatcctcc ccggcgtccc cggtgcggag acagacgtca tagagagcat cgagatcgcc
      661 tggctcaacc ggtcggatca acatggtcta atcggactat accaattcct ggcatgccca
      721 tcatggaaac gttgggattc catccaccaa gttgagacca atgcggtata gtcgccgccg
      781 gtgactgtat ccagccccga cggcaagacc caggaggcgg tgcgtcagtt gcgccatctc
      841 gtcggcacac tggaggcagg cagccgcatc ccgagcgagc gcgacctggc cgcccactgg
      901 ggtgtcgccc ggatgaccgc tcgcaaagcc atcgagacgc tgaccgcaga gggctggctg
      961 gatcggcgtc atggttcggg cacctacgtc gcccagatgc catatgccaa gacgctcggg
     1021 ctctcgtcgt tcaccgccga catggagcgc cgcggtctta tcccttccac tcgggtcatc
     1081 gactttgacc gggtacctgc ggattccgcc acggcagcac gtctcgacat cgacgaggga
     1141 gaggaggtgg tgcgattcac aaggctccgc ctcgccgacg acgagcccat tgcagtcgag
     1201 accacgtgga tcccggccca tctcgttccc ggcctcggca aagacgacct ttctggctct
     1261 ttgttcgaga cgctgagtca gaagttcgga atcactctcg gccaggcgtc gagcacgatc
     1321 gaccccgccc tggccgaccc gaccaccgca gctgagctcg acatcgaaga ccacgagccg
     1381 tgtctgcgga ttcaaatggg gtacagcgat caccgccggc gaacggtgat ggcagcgaca
     1441 tgcctctatc ggggcgaccg ctaccagatc cacgtcgtgc tcacccctgc cgccttcaac
     1501 tcggcgccga gatgagcgca ggagagcgat atctggcagg cgcgggcagg atcctcgaac
     1561 agattgccgc cgatgaatgg ccatcgattc gcatcgcggc ggagacggtt gcggactcga
     1621 tcgccggcga cggattggtc cacgtcttcg gaaccggtca ctcccacatg ctggccgagg
     1681 agatgttcta ccgagccggt ggactcgctg cgatcaatcc gatcctgatc gaggggctca
     1741 tgctccacgc tggggcggag aagagcaccc atctcgagcg acaacatggg ctcgccggcc
     1801 agatcttcag tgaccttccg ctgaagccgg gtgacacatt cctcgttgcc tccaactcgg
     1861 gaggcaatgc cgtgtgtgaa gagctggccc agctggccgg cgaagccggg gcagcagtga
     1921 tagccatcgt cagcagccgt cacgccgagc actcggcggc cgagcggaac gggtcccgtc
     1981 tcgccgacct ggccgatatc gtcatcgaca accacggcga tatcggggac gccggggtcg
     2041 acatcgatgg aatcgcccga agggtcgggc ccacttcgac ggtggccggc gcagcaatcg
     2101 tcaacgcaat cgtggcggag gcggtggaga tcctgctcga gcgaggagta gaagtcgacg
     2161 tcttcacctc ctccaatctg cccgagggcg acgccgcgaa cgcggagctg gtggcccgct
     2221 accgggatcg ggtgcgggcg ttgtgagcca cacggtgcgc tggctttcgg tcccacccga
     2281 gatcgaccgc gagcatctcg acgggatggc ccgccagtgt ggcctcactg tcgcagaccg
     2341 ggccgacacc aatctcactg tcgccgtcac cttgtcggcg gacccagacc tcacctacga
     2401 ctcggcccac aggcggatct ccgacgagac ctacatcctc cgcctcggtg aggaaggaca
     2461 tgcggggttg gatgtcaccg ggtcccgctc ccttcgctgg ggtctggtcg atctcgccgg
     2521 caaggccggc ggggaagact gggagagcgg tgagcgccga caaagccccg ggttcgggat
     2581 ccgaggcatc atcgagggct tctacggccc gccgtgggag gaccaggctc ggctcgacat
     2641 gatcgacttc gcttcgcgtc atcgcttcaa cactttcttc tactcaccca aggacgaccc
     2701 gtatttgcgt cgggactggc gcaccccctt cgaggggaag agcctgcggc gcctcgagga
     2761 agtgatcgag cgctgtcgtg accgcgatat cgattccatg atcggagtct cccccggcct
     2821 ctccatgcgc tactccgacg acaaggacaa cgatctgttg gcggagaaga tctcctcgct
     2881 catcgacctg ggcgctacca gcatcgccct cctgttcgac gacatcccgg accgcctcca
     2941 gcacgacgcc gacctgtcga cattccccga cctagccgcg gctcatgctc aaaccgccaa
     3001 cagggtcttc gacgggatag ccgggccctt cgtggtctgc ccaacgatct attggggagc
     3061 gggtgacgag gaatacatca gccgtctcgg cgatcaggtc gacccacgga tcgacctgtt
     3121 ctggacaggg cgcgcagtct gctcgcctgc catcacagtc accgaggccg cccacttcgc
     3181 ccgggtaaat cggcgaccgc cgctgtactg ggacaactac ccggtgaacg atgcctcgat
     3241 ggcgaatgag atgcacattg ggccctttca gaatcgggac ggtgccctcg accggttctg
     3301 ccgagggctt atggccaacg ccatggagta cccggaggct tcgaagatcg ggctcgccac
     3361 catcgccgac tatctgtggg acccggtcgg gtatgacccg gaaaccagct ggcaacgggc
     3421 catcgtcgag atcgccggcg ttacggatgc cccggctctg tctctgtttg ccgacacggt
     3481 ccgggccagc tgtctttccg atcccgaccc actcggtctc accgcggcgc tgcaggctta
     3541 cgccttccag atcgagagtg gccacccccg ggccgcggca acaggcttgg ccgcctatgg
     3601 actcgagctg accggcgccg ccgatcacct tcttggacgc gacatggaaa accgcgtgct
     3661 ccaggccgag ctcgccccat ggctggccaa gttccggtcc ggcgcggagg ctgtcacggc
     3721 gatcgcggca cgcaggggcg agggggatct caccgaatcg ctgaaacatc ttcgggaaga
     3781 cccccgtcgg gtcttcggcg acgtgctcga gatgacactc gccgatttga tcaaatgaga
     3841 gaaaggaaac caatgaaaca gagatatggt ccgctggcac tcttgctggc attgggcctc
     3901 gtcgtcgggg cgtgtgccga gggcgcaacc gatgacgcaa gcgacaccac gtcaactggt
     3961 gaaaccactg cggaaaccac gggctccgaa gaggaggtcg tcctccggat cgccatgggc
     4021 tctcccggcg aggagcagat cggggtctgg gaacaggcca aggccgcgtt cgaggaggag
     4081 aaccccaacg tcagtgtgga gctcaacttc caggacgacg atctctacca gaccatcggg
     4141 ctgctcaacc tgctcaacgg cggcgaagct cccgacatct acttcgagtg ggcgggagcc
     4201 cggctccaga gccgctacga cgaagggttc gtggccgaca tcaccgaggc ggtggacggc
     4261 gaactcggga gctatttcga cgagggcgcc ttcaacggca tggtgatcga cgacaagaac
     4321 gtgatggtgc cggccaacgc cgacgtgacc acggtgttct ggtacaacac cgagatcttc
     4381 gacgcagtgg gtgtcagcgt acccaccacc tgggaagagc tgatcgacgt ctccaactcg
     4441 atcaaggagg cagggatcat ccccatcgcc atcggcaaca aggacttgtg gccggtgggc
     4501 aactggacct cacacatcgt gtcccgcgtc atcggtgagg aggcctacga ccagatgatg
     4561 cgccaggaga tcccgctcga ctccccagag gtgatcgagg cgctgggcta tgtccagcaa
     4621 ctggctgatg ctgggatcgt caacgagagc gcaaacgcaa tcgacgacaa cgagggagct
     4681 cagctcttct tccagggtca ggcggcgatg catccgatcg ggagctggct cgtctcgtgg
     4741 gccctcgagg aggcgcccga tctcagcttc gattacttca acctgcccga ggtgcccggc
     4801 gcgggcaccc aggactcggt gatcggcgtc tcgacaggct tcgtcatcaa cgagaactct
     4861 gagcacaagg acgtcgccat cgacttcatg acgttgttct cttcgcccga gttcacctcg
     4921 ctgatggtcg atgccggtgg cacccccatc gccaccggtg ccttcgatcg ggatgatatc
     4981 gatcccaggg tcactgccat gatggagctg ctgcagggag cgagcgcctt ggtggcaccg
     5041 cccgacaccg gctatgacct cgaagtggcc gatgccctct acgccgccga ggcagaggtg
     5101 ctcggcgggg tggcgacacc ggaggaagcc gcggcggcgg ctcaggccaa gctggatcaa
     5161 taggaggaat cgagaggtag gagcagagga ggatgcggcc gcaacgccgg atcaccccct
     5221 acctgctggt ggcgccggcc ctggcactgt tcaccttcgc agtgctgggg ccgatcgtcg
     5281 ccaccggtgt gttcagtttc ttcgaatggg atgggttcgg cccgttcgac ccggtgggtt
     5341 tcgccaacta cctgcgggct gccgcagatc ccctgttccg ggcttcgttt gttcacgtcg
     5401 ccatctacat cgtcctgacc atcttcctcg aggtgttcgt cgggctgctt ctggcaggac
     5461 tcgtcaccgc gcgaccgaaa ggcaccgggc tcttccgggt ggccttcttc ataccggtgc
     5521 tgctgcccat ggtcgtcgtg gctgtgctct gggacttcgt ctacaacccc gacttcgggt
     5581 tgctcaactc gacactgatt gcgttcggcc tcgaggactt cacccagatc tggctcggtg
     5641 acccccgctt cgcactgacc gcggtgtctg tggtgtcggg ttgggttttt gccgggttct
     5701 acatggccat cttctacgcc gggttccggg gaatccccgg cgacgtcctg gaagcggcca
     5761 ggatcgatgg ggcgtcggag atccagacct tctggcgggt gaaggtgccg atgatccgcc
     5821 atgtcatcga aatcgcgttg ctcctgtgca tcaccggcgg tttccaggca ttcgaccttt
     5881 tctatgtgtt gaccaacggg ggtccgttca acgcgaccga gatcccgacc acctatctgg
     5941 tgcgggtggt gttccgaaac caggaagtcg gctacgggtc ggcgatggcg gtggtgatga
     6001 ctctggtggt gctgatcatc ggtggcctct acgtacgact gcgccggcgt actgcggtgg
     6061 agacggtgtc atgagacggg tcctggggag ccgggccgtg ttctatacgg tg
//
LOCUS       DASTXO010000304         2870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1823790, whole genome shotgun sequence.
ACCESSION   DASTXO010000304 DASTXO010000000
VERSION     DASTXO010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1823790"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VFU96_06575"
     CDS             <1..269
                     /locus_tag="VFU96_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEU5112967.1"
                     /translation="GERLHACILAAAVSTPFIGIEYRPKLRDFAESVDRGDLVVRTDS
                     LSGDSIVELAAKATSLGTEATDSLVAIYRSRLRQASAFLQKAVR"
     gene            320..1714
                     /locus_tag="VFU96_06580"
     CDS             320..1714
                     /locus_tag="VFU96_06580"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5112968.1"
                     /translation="MGHLTRQLAIALAIGDRAETTMFSLSPGFPLAMDFGIRGEYCPS
                     FTTQFIVRKEWDNYLQDRLAAFADEVRPDVLLFDGVAPYSGFMRAIAQLPDCVVGWLR
                     RGMWREGSNEHQLQKTAGFDFVIEPTDLASDIDTGATIGAAALRVPPISLLEVMDQLD
                     RETAALELGIDPDRPTLLLTLGSGRLGDVAGPGSTALDVVLGNTDWQVAVTNSPVAVN
                     LVPLVDDSRVIEIKGVYPLLRYFNAFDATVSAAGYNSVHELIPAGVPTLLIPNTSSKT
                     DNQEGRAQYLADLGVALSARDDDLTAVARETARLLDPAVRAQLIDNHHTTGLANRVGG
                     AKAVASELLTPPQPSGRERAQNHWDAPLSSRIAGAVLGERGKATVKKLLGRTVPDYTT
                     ERRRVRLGDPPVSPEDGILSLVITSERDRVDPRASYPIEHVLDGATPGYRQRRMEIVE
                     KYYEVVRPESAVSP"
     gene            complement(1704..>2870)
                     /locus_tag="VFU96_06585"
     CDS             complement(1704..>2870)
                     /locus_tag="VFU96_06585"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU5112969.1"
                     /translation="QSARWEVRLPKIQESGEVRRYRFGVRFSTRLLAGFVLALGSFLS
                     TSAPALAAPFDPSGMTFPLDGEYRLTDSFGDCRGSGCSRAHEGVDIMTDKGVPVLAVA
                     DGNVSWISSGPTDCCYLGIDHGGGWVTRYIHLNDDKKDAAGNYIDNTDGQGWGIAQGL
                     AEGIAVERGQVIGWAGDSGNAAETVSHLHFELRKNGTAIDAYPYLVNAIGGWDGRFRD
                     DDGNVHEKNIDIIAGWGITIGCNPPLNNMFCPGDHITRGQMAAFIARALDLPDGGHIP
                     YTDVAGTTFDAAIRAIELAGIGFGCTETKFCPDQDLLRHEMAELLVRAFGYDNPDGTD
                     FFVDDDGNRFENSINALANHRITMGCNPPANTNFCPDMALTRAQMATFFVRAMG"
BASE COUNT          517 a          931 c          897 g          525 t
ORIGIN      
        1 taggcgagcg gctccacgcc tgcatcctgg ccgcggcggt gtcgacgccg ttcatcggca
       61 tcgaataccg cccgaagttg agggatttcg ccgaatcggt ggatcgcggc gatctcgtgg
      121 tccggaccga cagcctgtca ggagactcca tcgtggagct cgcggcgaaa gcgacgagcc
      181 tcggcacgga agccaccgac tcgctcgttg ccatttatcg gagccgcctg cggcaggctt
      241 ccgcttttct gcagaaggca gtgcgctgac cgggccggct catccgcgca tcctcctcgt
      301 caccagcaac ggctgggggt tggggcatct cacccgtcag ctggcgatag ctctcgccat
      361 tggcgaccgg gccgagacca ccatgttctc gctatccccc ggatttcctc tcgccatgga
      421 cttcgggatc cggggcgagt actgcccgag cttcaccaca cagttcatcg tgcgaaagga
      481 atgggacaac tatctccaag accggctcgc ggctttcgcc gatgaggtga ggcccgacgt
      541 tctgctcttc gacggggtcg cgccgtattc cggattcatg cgggcgatcg ctcaactccc
      601 cgactgtgtg gtgggttggc tgcgccgcgg catgtggcgc gagggatcca acgagcatca
      661 gctccaaaag acagccgggt tcgacttcgt gatcgagccc accgatctcg cttctgacat
      721 cgacacgggg gccaccatcg gcgccgccgc cctgcgggtg ccgccaatca gcctgctcga
      781 ggtgatggac caactcgacc gcgagacggc ggcgctggag ctcggcatcg acccggatcg
      841 cccgacactt ctgctgaccc tgggctcggg ccggctcggc gatgtggccg ggcctggctc
      901 caccgccctc gacgtggtcc ttggaaacac ggactggcag gtcgcggtga ccaattcccc
      961 ggtcgcggtg aatctggtcc cgttggtcga cgattcacgc gtgatcgaga tcaaaggggt
     1021 gtatccgctg cttcgctact tcaacgcctt cgacgccacc gtcagtgccg ccgggtacaa
     1081 ctcggtgcat gaactgatcc cggccggcgt gcccactttg ctcattccca acacctccag
     1141 caagaccgac aaccaggagg gacgggctca gtacctagcc gacctcggtg tcgccctgtc
     1201 agctcgtgac gacgatctga ccgcggtggc gagggagacg gcacggctcc tcgacccggc
     1261 ggttcgggcc cagttgatcg acaaccatca cacgactggc ctcgccaacc gtgtcggagg
     1321 cgcaaaagcg gtggcttccg agctcttgac gcctccacaa ccgtcggggc gggaacgggc
     1381 ccagaaccat tgggatgcgc ccctctcgag tcggatcgcc ggcgcggtcc tcggcgagag
     1441 aggcaaggcc acggtcaaga aactgctggg ccgcaccgtc cccgactaca cgaccgaacg
     1501 acggagggtc cgcctcggtg acccgccggt ttcgccggag gacgggatcc tcagcttggt
     1561 gataaccagc gagcgtgacc gggtcgatcc acgcgcctcc tatcccatcg agcacgtttt
     1621 ggacggggcg actccgggct acagacaacg tcggatggag atcgtcgaga agtactacga
     1681 ggtggtgcgt cccgagtctg cggtcagccc atagcccgga cgaagaacgt cgccatctgg
     1741 gcgcgggtca aggccatatc aggacagaag ttggtgttgg ccggagggtt gcaacccatg
     1801 gtgatccggt ggttggccag cgcgttgatc gaattctcga atcggttgcc gtcgtcgtcg
     1861 acgaagaagt ccgtgccatc cgggttgtca tacccaaagg cccgcaccaa aagctccgcc
     1921 atctcgtgtc ggagcaggtc ctggtcgggg cagaacttgg tctcggtgca tccgaagccg
     1981 atgcctgcca gttcgatggc cctgatcgca gcatcgaatg tggtcccggc aacatctgtg
     2041 tacgggatat gacctccatc gggaaggtcg agggcacgcg cgatgaacgc cgccatctgc
     2101 cctctggtga tgtggtcccc cgggcagaac atgttgttga gcggtgggtt gcagccgatg
     2161 gtgatccccc atccagcgat gatgtcgatg ttcttctcgt ggacgttccc atcgtcgtca
     2221 cggaagcgcc cgtcccagcc accgatggca ttgaccaggt aggggtaggc atcgatcgct
     2281 gtcccgttct tgcgcaactc gaaatgcagg tgagaaaccg tctctgcagc gttgcccgaa
     2341 tcaccggccc agccgatgac ctgaccgcgc tccaccgcta tgccttcggc gagaccctgg
     2401 gctatacccc aaccctggcc gtcagtgttg tcgatgtaat tgccggcagc gtccttcttg
     2461 tcgtcattca gatggatgta gcgagtgacc catccgccgc cgtgatcgat ccccaggtag
     2521 cagcaatcgg tggggccgga gctgatccag ctgacattcc catcggcaac tgcgagcact
     2581 ggcacgcctt tgtccgtcat gatgtcgacg ccctcgtgag ccctggagca tccagatcct
     2641 cggcaatcgc cgaagctgtc ggtcagccgg tattcaccgt cgaggggaaa ggtcatgccg
     2701 ctggggtcaa agggtgcggc gagagcgggc gcgctcgtag aaagaaatga cccgagggcc
     2761 aacacgaacc ccgctaacaa acgcgtcgaa aacctcacgc cgaaccggta tcggcgcacc
     2821 tccccggact cttgaatctt cggaagacgg acctcccatc gcgcgctttg
//
LOCUS       DASTXO010000305         2308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1381648, whole genome shotgun sequence.
ACCESSION   DASTXO010000305 DASTXO010000000
VERSION     DASTXO010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381648"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="VFU96_06590"
     CDS             <1..933
                     /locus_tag="VFU96_06590"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III-family protein"
                     /protein_id="HEU5112970.1"
                     /translation="DRWASLLATGTALFGRLDWWPTPQVEGDVRSSVLASFASVGDLP
                     GGRPAGRPVTFPESGMTILRTAPEAAFEIWCRCDGGPLGYMATAAHGHDDALSVEIRH
                     DGVDVLADPGTYCYQSEPFWRSYFRSIRAHNTLELAKARHARQTGPFMWSSSERSDVV
                     EISEGPETSWTGICRRDHVDGGEIEHRRTVVLDEAGVTIEDSVDRSTPASLRFHLGPL
                     VECVLDGNLAQLTWASAEGQARSATMELDGAMTWRAVRGDEKAPLGWYSPGFDVRVAS
                     FTLEGAGELDPDVVVTTRLHMVDGEMAEAGESLR"
     gene            complement(937..2169)
                     /locus_tag="VFU96_06595"
     CDS             complement(937..2169)
                     /locus_tag="VFU96_06595"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5112971.1"
                     /translation="MASAGRRVLIIVQNLPVPFDRRVWLEATTLQAHGYQVSVICPKL
                     KGFNESRETIEDVDVYRYPMPFDPKSKLGFIAEFSWAWLATLVLSFKVGRLGRGFDVI
                     HACNPPETYWALALLWRIAGKRFIFDHHDLSPEMFEAKFGVKSGIVQRLLLFMERRSF
                     RTAEVVLATNESHRTVAIERGGMSAERVAIVRSGPDLKRLTVYPPDPAWAKSKKHVIA
                     YLGDISAQDGVDGLVRVVARLSEKRDDFHTVVIGGGSAWEDVKRYAHEQGVDDLFTFT
                     GVVSDDVLCRALSSATVGVDTVPKNSWSDRSTMNKIMEYMYFGLPVVAFDLTETIRSA
                     EDAVLTARSGDESDFAEILDQLLNDPERRMQMSTAGRRRLESNLSWEHSVPHLLRAYE
                     RAFANLPRGRLAARSRPV"
BASE COUNT          413 a          740 c          740 g          415 t
ORIGIN      
        1 gaccgatggg cctcgctgct cgccaccggg accgcgttgt tcgggcggct cgactggtgg
       61 cccacaccgc aggtcgaggg cgatgtccgg agctcggtcc tcgcctcgtt tgcctcagtc
      121 ggcgatctgc cagggggtcg gcccgcgggc cggccggtca cctttcccga atccgggatg
      181 acgatcctga ggacagcgcc agaggcggcg ttcgagatct ggtgtcgctg tgacggtggc
      241 ccgctcgggt acatggctac cgcggctcac ggccatgacg acgccctgtc tgtcgaaata
      301 cgacacgacg gtgtcgatgt gctggctgat ccgggtacct actgctacca gtcggagcct
      361 ttctggcgct cgtacttccg ctcgattcgc gcccataaca cgctcgagct cgccaaggcg
      421 aggcacgccc ggcaaacggg gcccttcatg tggtcctcgt ccgagcgatc tgatgtggtg
      481 gagatctctg aaggcccgga gacgagctgg acagggatct gcaggcgaga tcatgtggac
      541 ggaggggaga tcgagcaccg gaggaccgtc gtcctcgacg aagccggcgt gacgatcgag
      601 gacagtgtcg atcgatctac cccagccagc ctgcgcttcc acttgggccc actcgtcgaa
      661 tgtgtgctcg acgggaacct ggcgcagctg acgtgggcgt cagccgaagg ccaggcccgt
      721 tcggcaacga tggagctgga cggggccatg acctggcggg ccgtgcgcgg cgacgagaag
      781 gcgccgctcg gctggtactc gcccggcttc gacgtgcgtg tcgcctcctt caccctcgaa
      841 ggtgcaggcg agctggatcc cgatgtggtt gtgacgacac ggctccacat ggtcgacggg
      901 gaaatggctg aggccggtga atccttacgc tgaaggtcag acgggccgag acctcgccgc
      961 gagtcggcct cgcgggagat tggcgaacgc gcgctcatag gccctcagca gatgagggac
     1021 cgagtgctcc caactcaggt tgctctccag gcgacgccga cccgcggtgg acatctgcat
     1081 ccgtcgttcg gggtcgttga ggagctggtc gaggatctca gcgaaatccg actcgtcacc
     1141 ggatcgagcg gtgagcaccg catcctcggc ggaccggatc gtctcggtga gatcgaaggc
     1201 caccaccggc aggccgaagt acatgtattc catgatcttg ttcatggtgg accgatccga
     1261 ccagctgttc ttgggaaccg tgtccacgcc cacggttgcc gacgacagcg cccggcacag
     1321 cacgtcatct gacacgacac cggtgaaggt gaagagatcg tccacaccct gctcgtgtgc
     1381 gtaccgtttc acgtcctccc aggcggaccc gccaccgatg acgaccgtgt gaaagtcgtc
     1441 ccgtttctcg ctcaaccggg ccaccaccct gacgagaccg tcgacgccgt cctgcgccga
     1501 gatgtccccg aggtaggcga tgacgtgttt cttcgatttc gcccatgccg ggtcgggggg
     1561 atacacagtg agccgtttca gatcaggccc tgatctcacg atcgcgaccc tttccgcgct
     1621 catgccgccg cgttcgatgg cgacggttcg gtggctctcg ttggtggcca ggacgacttc
     1681 ggctgtccgg aaagagcggc gctccatgaa gagcaagagc cgttgcacga tgccactctt
     1741 cacgccaaac ttggcttcga acatctccgg agacaggtcg tggtggtcga agatgaacct
     1801 cttccctgct atccgccaaa gcagggccag ggcccaatat gtctccggtg ggttgcaggc
     1861 gtgaatcacg tcgaagccac gacccaggcg cccaaccttg aaggagagaa ccagggtggc
     1921 gagccacgcc catgagaact ccgcgatgaa gcccagcttg gactttgggt cgaacggcat
     1981 cgggtagcga tacacgtcga cgtcctcgat cgtctcccgg gactcgttga atcccttcag
     2041 cttggggcag atcaccgata cctggtagcc gtgagcctgc agcgtggtcg cttcgagcca
     2101 gacccgccgg tcgaaaggca ccgggaggtt ctggacgatg atcaggacac gcctaccagc
     2161 cgatgccaac gtaactccca tctaccggtc tgaccgacat ccctgccaga tcgaccaggt
     2221 gctgagccgg ccccagcttt agcgctgcgt cgaccaaggg ctcgtcggtc ttccccagga
     2281 cgactatgtc cccgaaagcc atcacctc
//
LOCUS       DASTXO010000306         3064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_129376, whole genome shotgun sequence.
ACCESSION   DASTXO010000306 DASTXO010000000
VERSION     DASTXO010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129376"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="VFU96_06600"
     CDS             complement(<1..978)
                     /locus_tag="VFU96_06600"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5112972.1"
                     /translation="MRVTPLDLSDAAAFAELHAVYRARMEHDLPDQAVLDTESLRAAM
                     SAAQPHTDLEHWVARIDGRIVGYVDLVLPLTENRTNIHSAGYVHPGQRRRGIGTALYR
                     QIVARAEALGRTNLTAMVDESTPGGFVRHDGAGAAFLLKQGLERVLVEYRRRLDIASV
                     DEQALESLDNEAQKKASDYEILTWSDRADDALAADLAHLEERLHIDLPTGDLDWDPLR
                     YDVDRWRQLEDMGARRLRTVHYAAARHRFSGKLAAYTGFMLPKQPRTHARQVTTVVLP
                     EHRGHRLGLLVKLANLRYAREREPRLRYIDTYNAEGNSHLIAINEAMGFV"
     gene            complement(1093..2775)
                     /locus_tag="VFU96_06605"
     CDS             complement(1093..2775)
                     /locus_tag="VFU96_06605"
                     /inference="COORDINATES: protein motif:HMM:NF021588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-hydrolase family protein"
                     /protein_id="HEU5112973.1"
                     /translation="MSSLALMEQTRVTKVFSRPVSRPGVLTGVLMFASSLQPSLLPKL
                     SITQGIVSGISFMVGYGLGAGWEWVWNFLGMPKPRGLWWTVLRWLALGLITFLFLVGM
                     WGFVGWQNDVRDTFGMDPISPMFWLTLIPVAFVVAALILIVTRALWRLFLLLIKWLDK
                     LFPRRLAVVLSGVGLLLTIWGGWSGVLVNSFFAVANQVFAPRDASTAEGITQPASELR
                     SGSPASLVGWATLGEKGRTFVATGPSVDELNAFHGDGAMEPIRVYVGLKSAATPEARA
                     ELVLDELIRTGAFDRDVLVVASTTGTGFLEPNAMTALEYVHNGNVAVAGAQYSYLPSW
                     ISLLADQDEVKETSQEVFATIHAYLSTLPEDERPEIYLYGLSLGAYGVEAILTSINIV
                     NEPIDGALMVGPPFVNELWNELVANRDPGSSPPTPLYQGGRTVRFTNEQNALERPGED
                     WGDTRVVYLQHASDPVVFFSPSLFLSQPDWLLEGQRGVEISEQFQYARLITGWQVMMD
                     LPAAGSIPEGYGHLYTKQANAEAWIAMTRPEGWSVGDTERLTEFLADLGPAD"
     gene            complement(2829..>3064)
                     /locus_tag="VFU96_06610"
     CDS             complement(2829..>3064)
                     /locus_tag="VFU96_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015659018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HEU5112974.1"
                     /translation="PDESGALGSAEAAWARRFLHALQPHRAGVYVNFLDSDDDPTRVR
                     EAYGDHTYRRLAEVKAKYDPDNVFHHNKNIPPG"
BASE COUNT          580 a          951 c          980 g          553 t
ORIGIN      
        1 aacgaagccc atcgcctcat tgatcgcgat caggtgagag ttgccctcgg cgttgtaggt
       61 gtcgatatag cgcagccgcg gttctcgttc acgtgcgtat cgcaggttgg cgagcttgac
      121 gagcagaccc agccggtggc cccggtgttc ggggagcacg acggtggtga cctgtcgggc
      181 gtgggtgcgg ggctgcttgg gcaacatgaa accggtgtag gcggccagct tgccgctgaa
      241 cctgtgccgg gcggcggcgt agtgaaccgt gcgcaatcgc cgggcaccca tgtcctccag
      301 ttgccgccac cggtccacgt cgtatctcaa cgggtcccaa tccaggtctc ctgtcggcaa
      361 gtcgatatgg agacgttcct ccaaatgggc aaggtccgcg gccaacgcgt cgtcggcgcg
      421 gtcgctccag gtcaggatct cgtagtcgga cgcctttttc tgtgcctcgt tgtccagcga
      481 ttccagggct tgctcatcca cgcttgcaat gtcgaggcgg cggcgatact cgacgaggac
      541 tcgctcgagt ccctgtttga gcaagaacgc cgcaccggca ccgtcatgcc gcacgaagcc
      601 accgggtgtc gactcgtcca ccattgcggt gagattggtg cggcccaggg cttctgctcg
      661 ggcgacgatc tgacggtaaa gcgcagtgcc gatgccccgt cggcgctgcc cgggatgcac
      721 atacccggca ctgtggatat tggtccggtt ctcggtcaag ggcagcacca ggtcgacgta
      781 acccacgatc cgaccgtcga tgcgggccac ccaatgctca aggtcggtgt ggggctgtgc
      841 ggcactcatg gccgcgcgca gagactcggt gtcgaggacc gcttggtccg gcagatcgtg
      901 ctccatgcgc gcccgataca cggcgtgcag ttcggcgaac gcagcagcat cggaaaggtc
      961 gagcggggtt acgcgcatcc agacgatcct ggctgtgccc tcggcgggat gccagcgaat
     1021 tatccgagtc gccgggttcg ggtcgcggtt ttcgatcccg acccttgccc tttcggcctg
     1081 gtcgatttcg ttctagtccg ccgggcctag atcagcgaga aactccgtca gtctttccgt
     1141 gtcgccaaca gaccaacctt cggggcgcgt catcgcgatc catgcctctg cattggcctg
     1201 cttggtgtag aggtggccgt aaccctcagg aatcgaacca gcggcgggga gatccatcat
     1261 cacctgccat ccggtgatga ggcgggcgta ctggaactgt tcactgatct ctacgccgcg
     1321 ctgcccttcg aggagccagt ccggctggct gaggaacaac gagggcgaaa agaagaccac
     1381 cgggtcggat gcgtgttgca ggtagacgac cctcgtgtct ccccaatctt caccaggtcg
     1441 ttcgagtgca ttctgttcgt tggtaaaacg aaccgttcgt cctccctggt agagaggtgt
     1501 cggcggcgag ctacccggat ctcgattcgc cacgagctcg ttccacaact cattgacgaa
     1561 cgggggaccg accatgagcg ctccgtcgat tggttcgttg acgatgttga tcgaagtgag
     1621 aatcgcctcg accccgtacg ctccgagcga caggccgtac aggtagatct cggggcgttc
     1681 gtcctcggga agagtcgaca ggtaggcgtg gatggtggcg aagacctcct gcgatgtctc
     1741 cttcacctcg tcttgatcgg cgagaagcga gatccaactg ggcaggtagg agtattgggc
     1801 cccggccact gcgacattgc cgttgtggac atattccaag gctgtcatcg cgtttggttc
     1861 gagaaagccg gtccctgtgg tgctcgccac gacgagaaca tcccgatcga aagctccggt
     1921 ccggatcagc tcgtcgagga cgagttcggc ccgcgcctcg ggagtggcag ccgacttgag
     1981 gccgacgtac acccggatcg gctccatcgc cccgtcacca tgaaacgcat tcaattcatc
     2041 gactgacggg ccggtagcca cgaacgtgcg tcccttctcg ccgagtgtgg cccaaccgac
     2101 aagcgaggcg ggacttcccg acctgagctc ggatgcaggc tgcgtgatcc cttcggctgt
     2161 cgaagcgtcg cgcggggcga agacctgatt ggccacggcg aagaaagagt tgaccaggac
     2221 gccgctccac ccgccccaga tcgtgagcaa gagcccgact ccactgagca ccactgcgag
     2281 acggcggggg aagagcttgt cgagccattt gatcaaaagg agaaagagcc gccacagcgc
     2341 tctggtgacg atcaggatca aggccgctac gacaaaggcg accgggatga gcgtcagcca
     2401 gaacatgggg gagatcggat ccatcccgaa ggtgtcccgc acatcgttct gccagccgac
     2461 gaaaccccac atcccgacca ggaaaaggaa cgtgatcagg ccgagagcca gccagcggag
     2521 cacggtccac cagagcccgc gcggcttcgg catgcccagg aagttccaga cccactccca
     2581 accggcaccc agcccatacc ccaccatgaa cgagatcccg gagacgattc cttgggtgat
     2641 cgagagcttc ggcaaaagtg atggctgcag cgacgaggcg aacatcagga cgccagtgag
     2701 cacgcctggc ctcgagaccg gcctcgagaa caccttcgtg accctggtct gctccatcaa
     2761 cgccaacgac gacacaccag gaacttacgc cgcctgcccg aacgctcacg cgacccgccg
     2821 accaagggtc agccgggagg aatgttcttg ttgtggtgga atacgttgtc ggggtcgtat
     2881 ttggccttga cctcggcgag tctccggtac gtgtgatcgc cgtaggcctc tcgaacgcgg
     2941 gtgggatcgt cgtcggaatc gaggaagttg acgtagacgc cggctcggtg cggctggagt
     3001 gcgtggagga accgccgcgc ccacgcggcc tcggccgaac cgagcgcgcc ggactcgtcg
     3061 ggga
//
LOCUS       DASTXO010000307         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_755733, whole genome shotgun sequence.
ACCESSION   DASTXO010000307 DASTXO010000000
VERSION     DASTXO010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755733"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1467
                     /locus_tag="VFU96_06615"
     CDS             <1..1467
                     /locus_tag="VFU96_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU5112975.1"
                     /translation="GGVAAPRQASFMAYFSSRGPTGLPASPDIIKPDVTAPGQQILAG
                     NSPAEGFDVGGELFQAIQGTSMSSPHGAGLFALLKQAHPDWSAAAAKSAMMTTARQDV
                     TKEDATTPADPFDMGAGHVDPSGPADAANSMFNPGIVYDAGFNEYLGFLCDAAPEVFA
                     NPAGTCGSLDAAGIPIESENLNYPSIGASGVPGTLTVERTVTNVSGGTLRLDAVVEEP
                     SGYDVTVDPSRLTIPAGQSRTFEVTFDNLSGPVGEWRFGSLTWEGQGFSARSPIAVAA
                     SKLDAPEVVTGTGVEGTASFDITFGYEGEYDATAHGLAADVPIVGSVAQDPDQVFDPN
                     DATGTTAHDFSLSSTAFFRLTLNTEDLTPPDEAIDIDLYLYKDGELVASSTAGGTVEV
                     IELTQPEDGTYTLFVHGWQTTGVTVGYQIHTWDVPLAADAGSLSIVSEPPDAVLGATE
                     TIVAGWSGLDPGTNYLGAVGHNDGAGLFDLTLVEVSTQ"
     gene            complement(1519..1971)
                     /locus_tag="VFU96_06620"
     CDS             complement(1519..1971)
                     /locus_tag="VFU96_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112976.1"
                     /translation="MRLATGVAVVVLMLVACGSARDSAATGETSTSTTVAPTTENENT
                     TMPTSEQEQLPIVAPAREDLARRLRVDPEDLEVVSVEEVTWPDGSLGCPEPGMAYTEA
                     LVEGSKVVLGYDERVYVYHAGDDDQPFLCPSDDKDGGYEFLPPPGLNE"
BASE COUNT          370 a          667 c          679 g          370 t
ORIGIN      
        1 ggcggcgtcg ccgcaccgag acaggcttcc ttcatggcgt acttctcgtc gcgtggtccc
       61 accgggttgc cggcatctcc cgacatcatc aagccggacg tgaccgcgcc ggggcagcag
      121 atcctggccg ggaactcacc ggccgagggg ttcgatgttg gcggggagct gttccaggcg
      181 attcagggca cgtcgatgtc gagcccacac ggggccggct tgttcgccct gctcaagcag
      241 gcgcatcccg actggtcggc ggccgcggcc aaatcggcga tgatgaccac ggcgaggcag
      301 gacgtcacca aggaggacgc cactacgcct gccgacccgt tcgacatggg agcgggtcac
      361 gtcgacccga gtggaccggc agacgcagcc aactcgatgt tcaaccctgg catcgtttat
      421 gacgccggtt tcaacgagta cctgggtttt ctctgcgacg cagcaccgga ggtgttcgcc
      481 aatccagcag gcacctgtgg ctcgctcgat gcagccggga tccctatcga gtccgagaac
      541 ctcaactacc cgtctatcgg ggcatcaggg gttccgggca cgctcaccgt ggaacggacc
      601 gtcaccaacg tgtccggagg gacacttcgg ctggatgccg tggtcgagga gccgagcggc
      661 tatgacgtga cggttgaccc gtctcgtctg acgatcccgg cgggccagag ccgcacgttc
      721 gaggtcactt tcgacaacct gagtggccct gtcggagagt ggaggttcgg ttcattgacc
      781 tgggaagggc agggcttctc ggcgcgaagc ccgatcgcag tggccgcctc caagctggac
      841 gcacccgaag tcgtgaccgg aacgggcgtc gaaggcacag cctcgttcga catcactttc
      901 ggttatgaag gcgagtacga cgcaaccgct catgggttgg cggccgatgt cccgatcgtc
      961 ggatcggtgg cacaagaccc cgaccaggtc ttcgacccca atgacgccac cgggacgacg
     1021 gctcatgact tctcgttgag cagcacggcc ttcttcaggc tcacgctcaa caccgaagac
     1081 ctgacgccgc cggacgaggc catagacatc gacctctacc tgtacaagga cggggaattg
     1141 gtagccagct cgacggcggg gggcacggtc gaggtgatcg agctgaccca gcccgaggac
     1201 ggcacctaca ccctcttcgt gcacggttgg caaaccaccg gcgtgaccgt cggctatcag
     1261 atccacacct gggatgtccc gctggcggcc gatgcaggat cgctcagcat cgtctctgag
     1321 ccgcccgacg cagtcctggg tgcgaccgag accatcgtgg ccggatggtc aggtttggat
     1381 cccgggacca actacctcgg cgcggttggt cacaacgacg gcgccgggtt gttcgatctc
     1441 accctggtcg aggtcagcac ccagtaacca gtgcacagcc atgacatgga ggggcgtctc
     1501 gggacgcccc tccattgctc actcgttgag tcccggagga ggtaggaact cgtagccgcc
     1561 gtccttgtcg tccgagggac ataggaacgg ctggtcgtcg tcaccggcgt gatagacata
     1621 gacccgctcg tcgtagccga ggacgacctt ggatccctcg accaacgcct cggtgtaggc
     1681 catgcccggc tccgggcacc cgaggctccc atcgggccag gtcacctcct cgacggagac
     1741 gacctcgagg tcctcgggat cgacgcgaag acgccgggcc agatcctctc gggccggagc
     1801 gacgatggga agctgttcct gttcgctggt gggcatggtg gtgttctcgt tctcggtggt
     1861 tggtgcaacc gtggtcgacg ttgatgtctc gccagtcgcg gctgaatcgc gggcggaccc
     1921 gcacgccaca agcatcaaaa cgaccacggc aacaccggtc gcaagtcgca tggttctctt
     1981 catcaaagat aggacgccgt gggggtatcg acggttccga ccggcctttg ccccgtccct
     2041 ggtctgttgt gtagggtccc gacgggtcgg tatggggttg ctatcc
//
LOCUS       DASTXO010000308         3914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1345421, whole genome shotgun sequence.
ACCESSION   DASTXO010000308 DASTXO010000000
VERSION     DASTXO010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345421"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VFU96_06625"
     CDS             complement(<1..249)
                     /locus_tag="VFU96_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112977.1"
                     /translation="MTAETVGRIREVDWSTWLRRRGWTVGVWVLLVLMVAWYAALIPK
                     FGGFQIASISKNSLPLVYLAIGQAIVVIAGGIDLSVGAM"
     gene            complement(246..1715)
                     /locus_tag="VFU96_06630"
     CDS             complement(246..1715)
                     /locus_tag="VFU96_06630"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEU5112978.1"
                     /translation="MTLLATRAVAKSFGNVIALRSVDLEVAPGEVHALLGANGAGKST
                     FVKILSGVHQADSGEVEVEGKSVRLASPSAAVEAGLATVFQDPALIPDLTVAQNLRLT
                     DIDETDVREWLKRMEVTGLDFDALIRELPLPVLRLLDLARALALEPSLLLLDEITAAL
                     PADQAEHVFTVMEDWRKRGRSVLFITHRLGEVMRMCDRATVLRDGRTVASVIPLQVGE
                     SGLVEAMLGEVAHVEPGAAVRTDRRGVGHIALEAVDLASGDKVNGVSFQLHAGEVLGV
                     AALEGQGQDRLFEVLAGDRRPDGGEILVNGEPLQARSPFDAVSRGVVLVPPDRLLALL
                     PKRPVRENLGLPLYNRMKSWFSLARDEKERVANAIDRLSIDTRAARQAGRLSGGNQQK
                     VVVGRWLAAGFRVLLCFDPTRGIDVKTKAQIYDLLRELAADGAAILLYTSELAEIPLV
                     CDRVQVLYRGAIVDEQAADTATEESLLTAAHGISAEEVA"
     gene            complement(1787..2824)
                     /locus_tag="VFU96_06635"
     CDS             complement(1787..2824)
                     /locus_tag="VFU96_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007254619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5112979.1"
                     /translation="MKKRTGLRTAALIGVLALIAAACGGGESAEDETYTIGVSNTLAG
                     NGWREQMICAIKAQALASGVVDEVVLQNENTDTTGQIGQIEALISQGVDAIIINPTER
                     EGLNSVIEEAINQGIVVVAVDQAVTAEGAYVATNDQVAYGELGARWLFEQLGGEGNVV
                     EMRGIDGVPADTDRHTGFEAALADFPGITVTQETFTGWDPTTGAQQALDIISAGDVDG
                     IWTSGIDYTVVEQFQNADAPLVPIVGADNNGFVDQLLNVEGLEGAAVTNPPAIGGVGA
                     AIAIQALQGEEPDQTVTLTPEVFASDDQAALEELFDPEQQVGWSTYVEIDPWTTYEPD
                     QVSACVGPGEE"
     gene            complement(2902..>3914)
                     /locus_tag="VFU96_06640"
     CDS             complement(2902..>3914)
                     /locus_tag="VFU96_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU5112980.1"
                     /translation="GGAPSTGGRPPSLLGFNADAGLILAGDLGAPHSRIAVCNLLAES
                     LAETTAEIDINSGPDVVLAWLEETFDGLLADIRRSPREVRGVGIGVPGPVDYAHGVAV
                     NPPIMAGWHMHPIKDRFAARYGAPVLVDNDVNIMALGEYWVMEPKVDDFVFVKVGTGI
                     GSGLILGGVLHRGANGAAGDIGHVRASSDDVVCRCGNNGCLEALAGGAAIAGRLSDSG
                     LEAAGSRDVVQLVERGNELATRSVRDAGRLIGQVLASMVNLLNPSMIMIGGDLAKAEQ
                     QLLAGIREVVYQRSTTLSTTDLTITVSSLGDRSGVIGAAAMAIDHIMTPRAVDEALAG
                     AG"
BASE COUNT          675 a         1330 c         1228 g          681 t
ORIGIN      
        1 catcgcaccg accgagaggt cgatcccgcc ggcgatgaca acgatcgcct gcccgatcgc
       61 cagatacacg agcggcaggc tgttcttcga gatcgaggcg atctggaagc cgccgaactt
      121 ggggatcaga gcggcgtacc aggcaaccat gaggaccagg aggacccaca ccccgaccgt
      181 ccagccacgg cggcgcagcc aggtcgacca gtcgacctcc cggatgcgac caaccgtttc
      241 ggcggtcatg ccacctcctc tgcgctgatg ccgtgcgccg cagtcagcaa cgactcttca
      301 gtggcggtat cagctgcctg ctcgtcgacg atggcgccgc ggtagaggac ctgaacgcga
      361 tcgcacacga gagggatctc cgccagctcg ctggtgtaga gcaatatggc tgcgccatct
      421 gcggctagct cgcggaggag gtcatagatc tgagccttgg tcttgacgtc gatcccgcgg
      481 gtgggatcga agcagagcag aacccggaac ccggcagcga gccagcgccc gaccaccacc
      541 ttctgttggt tgccgccgga taagcgaccc gcctggcgcg ccgccctggt gtcgatggag
      601 agtcgatcga tggcattcgc cacccgctcc ttttcgtccc gtgccagcga aaaccaactc
      661 ttcattcggt tgtagagggg aagcccgaga ttctcccgca ccgggcgctt gggcaggaga
      721 gcaagaagac ggtctggcgg gaccaacacg acaccgcgcg aaaccgcgtc aaagggcgac
      781 cgtgcctgca gcggctcccc gttcaccaag atctctccgc cgtcaggtcg gcggtcaccc
      841 gccagcacct cgaacaatcg atcctggccc tgaccctcga gagcggccac gcccaacacc
      901 tcaccggcat ggagttggaa ggacaccccg ttcaccttgt ccccggaggc gaggtccacg
      961 gcctcgaggg cgatatggcc gacccctctc cggtctgtcc tcaccgcagc gcccggctcg
     1021 acgtgggcga cttcaccaag catcgcttcg accagacccg actcgccgac ctggagaggt
     1081 atgactgaag cgaccgtccg cccgtccctg aggaccgtcg cccgatcaca catccgcatc
     1141 acctcaccga gtcggtgggt gatgaagagg accgagcggc cccgcttcct ccaatcctcc
     1201 atgaccgtga acacgtgctc ggcttggtcg gcgggaaggg ccgcggtgat ctcgtcgagc
     1261 agaagcagcg atggctcgag ggcgagtgcc cgggccagat ccagcagccg aagaacgggc
     1321 aggggcagtt cccggatgag ggcatcgaaa tcgaggccgg tcacttccat ccgcttcagc
     1381 cattcacgaa cgtcggtttc atcgatgtcc gtgagtcgca ggttctgggc aaccgttaga
     1441 tccgggatca aggccggatc ctgaaacact gtggccaagc cggcctcgac ggcggcggat
     1501 ggcgacgcca ggcggaccga cttgccctct acctcgactt ccccgctgtc cgcctgatgc
     1561 accccggaga ggatcttgac gaaggtcgac ttgccggcgc cattggcgcc gagcaaggcg
     1621 tgcacctcac cgggggcgac ctcgaggtcg acagagcgca atgcgatgac gttgccaaag
     1681 ctcttggcaa ctgcgcgcgt ggctaacagg gtcatcgccg gctaatactc ccaggtcgac
     1741 cgttgaccgg ggcgccgaaa cgccccggtc aactggccgt tctcggctat tcctcgcccg
     1801 ggcccacgca ggccgaaacc tgatcgggct cgtaggtggt ccaagggtcg atctcgacgt
     1861 aggtgctcca cccgacctgc tgttccgggt cgaagagctc ctcgagcgcg gcctggtcat
     1921 cggaggcgaa gacctccgga gtgagagtca ccgtttgatc cggctcctcg ccctgcagcg
     1981 cttggatcgc gatcgcagca ccaacgccgc cgattgccgg agggttggtc actgcggcgc
     2041 cttccagccc ctccacgttg aggagttggt cgacgaaccc gttgttgtcg gcgccgacga
     2101 tcggcaccag cggggcatcc gcgttctgga actgctccac gacggtgtag tcgatcccgg
     2161 acgtccagat cccgtcgacg tcaccagcgc tgatgatgtc cagcgcttgt tgtgcgcccg
     2221 ttgtcgggtc ccaaccggtg aatgtctcct gggtgactgt gatcccgggg aagtcggcga
     2281 gagccgcttc gaagccggtg tgacggtcgg tgtccgcggg gacaccgtcg atgccacgca
     2341 tctcgaccac gttgccctcg cccccgagct gctcgaagag ccaacgagca cccagctcgc
     2401 cataggcgac ttggtcgttg gtggccacgt aggcaccctc ggcggtcacg gcctggtcga
     2461 ccgctaccac cacgatgccc tggttgatgg cctcctcgat gaccgagttg aggccctcac
     2521 gctcggtggg gttgatgatg atcgcgtcga ccccctgcga gatcagagct tcgatctggc
     2581 cgatctggcc cgtggtgtcg gtgttctcgt tctggagcac cacctcgtcc acaacgccgc
     2641 tggccagggc ctgggccttg atcgcgcaga tcatctgctc acgccagccg ttaccggcca
     2701 gggtgttgct gacaccgatc gtgtaggtct cgtcctcggc gctctcgccg ccgccacaag
     2761 ccgctgcgat cagagccagg acaccgatca gggccgcagt gcgcagtcct gtcctcttct
     2821 tcatccttcc tcctcttcct ttgttctgcc gctctcgcgg cgtttgctca ttcatcccgc
     2881 gtggcgggag aagactttgg ctcatccggc accagccaga gcttcgtcga cggccctcgg
     2941 cgtcatgatg tggtcgatgg ccatggcggc ggcgccgatg acaccggatc ggtctccgag
     3001 cgacgacacg gtgatggtca agtcggtcgt gctgagggtg gtcgatcgct ggtagacgac
     3061 ttctctgatc ccggccaaga gttgttgctc ggccttggcg aggtctccgc cgatcatgat
     3121 catcgacggg ttgagcagat tgaccatcga ggccagcacc tggccgatga gccggcccgc
     3181 gtccctcacc gagcgggtag ccagctcgtt ccctcgctcc accaactgca ccacgtcgcg
     3241 actgcccgcg gcctcgagac ccgaatcact caaccgaccg gcgatggcgg ccccgccggc
     3301 gagtgcttcg agacagccgt tgttgccgca tcggcacaca acatcgtccg agctggcccg
     3361 gacgtggccg atgtcaccgg cggccccatt tgcgccgcga tgcaggactc ctccaaggat
     3421 caatcctgag ccgatgccgg ttcccacctt gacgaagacg aaatcgtcca ctttcggctc
     3481 catgacccag tactcgccca gggccatgat gttcacgtcg ttgtcgacca ggaccggcgc
     3541 cccgtacctc gcggcgaaac ggtctttgat cgggtgcatg tgccagccgg ccatgatcgg
     3601 tgggttgacc gcaacgccgt gggcgtaatc caccgggccg ggcaccccga tcccgacgcc
     3661 gcgcacttct ctcggtgatc tcctgatgtc ggcgagaaga ccgtcgaagg tctcctcgag
     3721 ccaggccagc accacgtcgg gcccgctgtt gatgtcgatt tcggccgtgg tctcggccaa
     3781 agactcggcg aggagattgc acaccgcaat ccgggagtgg ggagcgccca ggtcgccggc
     3841 gaggatcaac ccggcgtctg cattgaagcc aaggagcgag ggtgggcggc cgccggtgga
     3901 tggagcgcca ccga
//
LOCUS       DASTXO010000309         4684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1824184, whole genome shotgun sequence.
ACCESSION   DASTXO010000309 DASTXO010000000
VERSION     DASTXO010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1824184"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VFU96_06645"
     CDS             complement(<1..546)
                     /locus_tag="VFU96_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5112981.1"
                     /translation="MKPIRWGRVLVAAVPALFIGYFFIYPLARILTLGLSELNIGSDG
                     VEARLFQIGWFTLWQAVVSTVLTLLAAVPMTWAVSHYEFRGRKLATALVTVPFVWPTV
                     VVGTAFVALGWRDSVWAILAAHVFFNIAVVVRTVGALWSRIDPDLHDAGRVLGAGEWK
                     VFRTVTLPLLRPAIAAAAAIVF"
     gene            complement(546..1586)
                     /locus_tag="VFU96_06650"
     CDS             complement(546..1586)
                     /locus_tag="VFU96_06650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA];
                     GO_process: GO:0030974 - thiamine pyrophosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5112982.1"
                     /translation="MKLRKTLALLVMIAVTAACGPDEAETPETLRLMAHDSFAGSVNE
                     ETFAPFTEATGIAVEVIAAGDAGSMVNQAALSKDNPLADVLFGVDDTFLSRAVEEGIF
                     AEHSSDLLVDVIGGLHSEIDVVTPIDFGDVCLNYDKAWFEENQTPVPTDLDQLRDPAY
                     AKALTVEHPATSSPGLAFMLATVRDFGEEGWLDFWSDLREGGVRVVPDWDTAYYNDFT
                     RYGGGSPMVVSYASSPPAEVIFATEPLEEAPTGVIEAGCYRQVEYAGVLAGTEYPQYA
                     GDLIDFMLSVEFQETIPLKWFVFPANRNADLPPEFTENTIVPDATERFTPETIARNRD
                     RWIDEWVAVMEG"
     regulatory      complement(1603..1714)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1734..2906)
                     /locus_tag="VFU96_06655"
     CDS             complement(1734..2906)
                     /locus_tag="VFU96_06655"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEU5112983.1"
                     /translation="MSRRKVVVTGIGVVAAPGVGMDDLWKGLQHPPSAPGFQLVANWD
                     PEPWLPKREHRRLDRFTQFALVAAHEALEQAGPLDADPTRVTVSVATGIGGLESLEKL
                     VHIADADEPRASPFLIPMMMANAAAAGISIKYGFGGQVSTPSVACAAGTQAVLDGLRQ
                     IQWGYADVAVVGGTEAAARKSAAEGFKAARALSPSGIARPFDVDRDGFVMGEGAGILV
                     LETEEAAIERGATILAEIRGGASTADAHHITAPHPGGEGAERAIRLALADAGLTPHDV
                     HYVNAHGTGTELNDRTEGGVIDRVFDVQPAVSSIKGTTGHAMGASGSIEAAVVVEAIR
                     RHELPPNVGLTNLDPEIPLDDVVYEPREWEPAAAITNSFGFGGHNTVLAFTPPATP"
     gene            complement(2929..3699)
                     /locus_tag="VFU96_06660"
     CDS             complement(2929..3699)
                     /locus_tag="VFU96_06660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019608244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5112984.1"
                     /translation="MNITDRVVIVTGGGSGIGAAMARRFAEERPRGLIVADLDLVAAE
                     MVASGIGATPLHVDVSDPDSNDRLIDETENRYGPVDLFCANAGIGFAGDEQTAPDRWD
                     FMWQVNLMSHVHAARRLIPGWVARGEGYFLSTASAAGLLTNLKAAQYSVTKHAAVAFA
                     EWLAVTYGDQGIKVSCLCPMGVKTPLLDSADEFHALLDPLALEPDVVADAVVAGLSEE
                     HFLILPHSEVSQFFQNKANDYDRWIGGMKKLQRTVFPT"
     gene            complement(3696..>4684)
                     /locus_tag="VFU96_06665"
     CDS             complement(3696..>4684)
                     /locus_tag="VFU96_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEU5112985.1"
                     /translation="VMSYGDDGTGAVGHLIGEEQEGMRNMFTMMNMARVGVGMEGVAI
                     GERAFQQALHHARQRVQGRSIGGESKTSVPIVEHPDVRRMLLTMKAYVEAMRSLLYVT
                     AAEADHLEHADSEDRRKLAADRLALLTPIVKAWCTDVGVEIASLGVQVHGGMGYIEET
                     GAAQFLRDSRIAPIYEGTNGIQAIDLVLRKIPLENGAVVAGVIGEMTAELAAMEGHDQ
                     LTGFREELSVAIQGLAETSSWLGEKLGAGDIQAALAGASPYLKQFGIVLGGWLMARTA
                     VAALRAPPGFEPSTLADKVTTARFYGEQLLPMANGLAPAVKGGASLLESASF"
BASE COUNT          851 a         1548 c         1478 g          807 t
ORIGIN      
        1 gaaaactatg gcggctgcgg cggcgatcgc cggtcgtaac aggggcaggg tgaccgtccg
       61 aaacaccttc cactcgccgg ctcccaacac gcgaccggca tcgtggagat ccgggtcgat
      121 tcgtgaccag agcgccccta cggtccggac gacgacggcg atgttgaaga agacgtgggc
      181 ggcgaggatg gcccataccg aatctctcca gcccagcgcc acaaaggcgg tgcctacgac
      241 cacggtgggc cacacgaagg ggaccgtcac cagcgccgtc gccagcttcc gcccgcggaa
      301 ctcgtagtgg gagacggccc aggtcatcgg caccgccgcc aataaagtca gcactgtcga
      361 cacgacggcc tgccagagag tgaaccatcc gatctgaaag agccgggcct cgacgccatc
      421 tgagccgatg ttcagctcgg agaggccgag ggtcaggatc ctggccaggg gatagatgaa
      481 gaaatacccg atgaaaaggg ccgggacagc tgcgaccagc acccggcccc agcgaatcgg
      541 cttcatcaac cctccatcac ggcgacccat tcgtcgatcc accggtcgcg gtttcgggcg
      601 attgtctccg gggtgaatcg ctcggtggca tcggggacga tcgtgttctc ggtgaactcg
      661 ggcggcaagt cggcgttccg gttcgccggg aagacgaacc atttcagggg aatcgtctcc
      721 tggaactcga ccgacaacat gaaatcgatc aggtcacctg cgtattgcgg gtactcggtc
      781 ccggccagca cgccggcgta ctcgacctgg cgatagcacc ctgcctcgat cacgccggtc
      841 ggcgcctcct cgagcggctc ggtggcgaag atgacctcgg ctggaggcga cgaggcgtag
      901 gacaccacca tcgggctccc gccgccgtat ctggtgaagt cgttgtaata ggcggtgtcc
      961 cagtccggca cgaccctcac tccgccctct cgcaaatcgc tccagaagtc gagccagccc
     1021 tcctcgccga agtcacgaac cgtggccaac atgaaggcca gacccggcga agaggtcgcc
     1081 gggtgttcca cggtgagcgc ctttgcatat gcgggatctc gcaattggtc gagatccgtc
     1141 gggaccggtg tctgattctc ctcgaaccag gccttgtcgt agttgaggca gacatctccg
     1201 aaatcgatcg gggtgaccac atcgatctcg gagtggagcc caccgatgac atccacgagt
     1261 agatcgctcg aatgctcggc aaagatcccc tcctcgacgg cccgggacaa gaaggtgtcg
     1321 tccacgccga acaggacgtc ggcgaggggg ttgtccttgg acagggcggc ttggttaacc
     1381 atcgacccgg cgtcaccggc ggcgatcacc tccacggcga tcccggtggc ctcggtgaag
     1441 ggggcgaatg tctcctcgtt caccgaacct gcgaacgagt cgtgggccat gagacgcagc
     1501 gtctccggcg tctcggcctc gtccggacca cacgccgcag tgacagcgat catcaccaac
     1561 aaggccagag tcttccgcaa cttcatcagc tcctcgttca tcgggagcga tgaggcgggt
     1621 ccctacgccg gcattacccg ggtcaggttc atcggtcggg atctccacag atcccctctc
     1681 agcccggtct caccgagctc ccgttcagag gcccaggtta aacgtcttgc gcactacggc
     1741 gtcgctggag gggtgaaggc aaggacggtg ttgtgcccgc cgaagccgaa agagttggtg
     1801 atggcggcgg caggttccca ctctcggggc tcgtagacga cgtcatcgag cgggatctcc
     1861 gggtcgaggt tggtcaggcc gacgttgggt ggcagctcgt ggcgccgtat cgcctcgacg
     1921 acgactgcgg cctcgatgga gcccgatgct cccatcgcat gcccggtggt cccctttatc
     1981 gatgacactg ccggctgaac gtcgaaaacg cggtcgatga caccgccttc ggtgcggtcg
     2041 ttgagctcgg tcccggtgcc atgggcgttc acgtagtgca cgtcatgggg ggtgaggccg
     2101 gcatcagcca gagcgagacg gatcgcccgt tccgctccct ccccacccgg gtgaggagcg
     2161 gtgatgtgat gggcgtcggc tgtcgaggca ccgcctctga tctcggccag gatcgtggct
     2221 ccgcgttcga tagcggcttc ctcggtctcg agtaccagga tccctgcccc ttctcccatg
     2281 acaaacccgt cgcggtcgac gtcgaacggt cgggcgatcc cgctcggcga cagcgccctc
     2341 gccgccttga acccctccgc ggcggacttg cgcgccgccg cctcggtgcc tccaaccacc
     2401 gccacatcgg cataacccca ctggatctgt cgaagcccgt cgagcacagc ttgggtgcct
     2461 gcggcgcagg ccactgaagg cgtggacacc tggcccccga atccgtactt gatcgaaatc
     2521 ccggccgcag cggcgttggc catcatcatc gggatgagga agggggaggc ccggggctcg
     2581 tcggcatcgg cgatgtgaac cagcttctca agtgattcga ggccgccgat gcccgtcgcc
     2641 accgacacag tgacccgggt tgggtctgca tcgagcgggc cggcctgctc cagggcctca
     2701 tgagcggcga cgagggcgaa ttgggtgaat cggtcgagac gtcggtgctc gcgcttgggc
     2761 agccagggct cgggatccca gttggcgacc aattggaacc caggtgccga tggagggtgc
     2821 tgcaggcctt tccacagatc atccatcccg acaccgggtg cggcgaccac accgataccg
     2881 gtgaccacca cctttcgcct gctcatctgc tcaattctgc ctgaaacatc acgtcgggaa
     2941 aaccgtccgc tgcagtttct tcattccccc aatccagcgg tcgtaatcgt ttgccttgtt
     3001 ctggaagaac tgactcacct ccgaatgggg gaggatgagg aagtgttcct cggacaaccc
     3061 agccaccacg gcgtcggcca ccacatcagg ctcgagggca agcgggtcga gcagcgcgtg
     3121 gaactcgtcg gccgaatcga gaagcggcgt cttgaccccc atagggcaca aacagctcac
     3181 ctttatcccc tggtcgccgt aggtcacggc gagccattcc gcgaaggcga ccgcggcgtg
     3241 cttcgtcacc gaatactgcg ctgccttcag attcgtcagg agcccagccg ccgaggccgt
     3301 ggagagaaag tacccctctc ccctggccac ccatcccggg atgagacgac gcgcggcatg
     3361 gacatgtgac atcaggttca cctgccacat gaagtcccag cgatccggcg cagtctgctc
     3421 atcaccggcg aacccgatac cggcgttggc acagaagaga tcgaccggcc cgtagcggtt
     3481 ctccgtctca tcgatgagac ggtcattcga atccgggtcg gagacgtcga cgtgcagagg
     3541 cgtcgcaccg atcccggatg ccaccatctc ggctgcgaca agatcgagat cggccacgat
     3601 gagaccgcgc ggcctctcct cggcgaatcg ccgtgccatg gcggcaccga tgccgctgcc
     3661 cccaccggtg acgatgacca cccgatccgt gatgttcaaa aggaggcgct ttcgagcagc
     3721 gaggcaccgc ccttgaccgc gggtgccagg ccgttggcca tcggcaacag ctgctcaccg
     3781 tagaaacgcg ccgtggtgac cttgtccgcc agagtggagg gctcgaagcc gggtggcgcc
     3841 ctcagggcgg cgaccgcggt gcgggccatc agccatccac cgaggacgat cccgaactgc
     3901 ttcagatatg gcgaggctcc ggccagcgcc gcctggatgt cgccagcgcc gagcttctcg
     3961 ccgagccagc tcgaggtctc ggccagcccc tggatggcga ccgacagctc ctcgcggaag
     4021 ccggtcagct ggtcgtggcc ctccatggcg gcaagctctg cggtcatctc gccgatgacg
     4081 ccggccacga cggccccgtt ctcgaggggg atctttcgga ggacgaggtc gatggcctgg
     4141 atgccgttgg tgccctcata tatcggtgcg atcctcgagt cgcgcaggaa ctgggcggct
     4201 ccggtttcct cgatgtatcc catgccgccg tggacctgta ccccgagtga ggcgatctcg
     4261 acccctacat cggtacacca ggccttgacg atcggagtga gcaaagcgag gcgatcagct
     4321 gcgagcttgc gtcggtcctc actgtcggca tgctcgaggt ggtcggcctc ggctgcggtg
     4381 acatagagga gagaacgcat cgcctcgacg taggccttca tcgtgagcag catgcgccgg
     4441 acatccgggt gttcgacgat tggaacgctt gtcttcgact cgccaccgat ggagcgtccc
     4501 tggaccctct gtcgggcgtg atggagggct tgctggaagg cacgctcgcc gatggctact
     4561 ccctccatgc cgacaccgac ccgagccatg ttcatcatcg tgaacatgtt cctcatgccc
     4621 tcctgctcct ccccgatgag atgaccaacg gctcccgtgc catcgtcgcc gtacgacatc
     4681 acac
//
LOCUS       DASTXO010000310         2162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_498325, whole genome shotgun sequence.
ACCESSION   DASTXO010000310 DASTXO010000000
VERSION     DASTXO010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498325"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VFU96_06670"
     CDS             complement(<1..236)
                     /locus_tag="VFU96_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017240657.1"
                     /note="catalyzes the thiolytic cleavage of
                     beta-ketoadipyl-CoA to succinate and acetyl-CoA; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HEU5112986.1"
                     /translation="MSGDIYIVDAARTPMGRIGGQLAHVRPDDLAAHVVAAVLRRQPD
                     LDPMAIEDIFWGAANQAGEDNRNVARMAGLLAGLP"
     gene            454..1014
                     /locus_tag="VFU96_06675"
     CDS             454..1014
                     /locus_tag="VFU96_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014741922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="HEU5112987.1"
                     /translation="MDRPDLLPFTESDEANRLLAADGFALLLGMLLDQQFPMERAFYG
                     PQLLKDRLGEELSPESVVSKPIEDLEVMFRGPPAIHRYPASMAKRAQELAHAVIDDYD
                     GRAENLWDTAEDGADLLSRLRALPGFGAEKSRIFVGLLGKRLGVRPEGWEEKAADWPS
                     IADVANFEDVLALREKKKAMKAAKKA"
     gene            1076..1936
                     /locus_tag="VFU96_06680"
     CDS             1076..1936
                     /locus_tag="VFU96_06680"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEU5112988.1"
                     /translation="MHLSGQLSDWSINDLLQIMQVTKKTGSLDIEGQRRGRVHFREGR
                     VTGAELTGFEGAHAGSDLASVADILYVLSTVAEGSFSVGAADGPDAKGWTVEEVLVDV
                     ESLQTLEGEVAETGLFEANGVKLINEIGESVTLDPTDWQTFANLVPAFSFGALEARYG
                     RGTAVRMFHTLSRMGLVEAVAIPEEEAEWLDRLAEGVAANSSWNGSDGETEVDTAAEP
                     ELEIDEEPIEPAAVEEPEITEPEPISVSISKPGRANVELKGVSAPASTTLTDGVYDEI
                     RRLRSRVGEK"
     gene            1948..>2162
                     /locus_tag="VFU96_06685"
     CDS             1948..>2162
                     /locus_tag="VFU96_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEU5112989.1"
                     /translation="MPARTNLGSDLPLIVGIAGGSGSGKTTIAESLVEKLNGHVALVQ
                     HDAYYRHTPELSFEARSQVNYDHPASL"
BASE COUNT          412 a          648 c          722 g          380 t
ORIGIN      
        1 gggaggccgg cgaggaggcc cgccatacgg gccacgttgc ggttgtcctc ccccgcctgg
       61 ttggccgccc cccagaagat gtcttcgatc gccatcgggt cgagatccgg ctgacgtctg
      121 agcaccgcgg cgacgacatg agcggcgagg tcgtcgggtc tgacatgtgc gagctgccca
      181 ccgatcctgc ccattggggt gcgggccgca tcgacgatgt agatgtcacc actcattctc
      241 gatcagttca cattctcgcc aattgctccg ggtcaaatgg gggggaaccc ctatatcccg
      301 cggaatcttc gagccgtagg ttcggttcaa gaggacgata gagatgaacg agccccgcag
      361 agacccgacc agcatggtgg cctacgcacc agcgggcctg ctcgtcctct acgtcatcat
      421 tcgcctcatg gtctgagggc tacggttggt ccgatggacc gacctgacct cctccctttc
      481 accgaatccg acgaggcaaa tcgactgctc gcggccgacg ggtttgctct gctcctgggc
      541 atgcttctcg accagcagtt cccgatggag cgcgccttct atggcccaca gctcctcaaa
      601 gaccggctcg gagaggaatt gtccccggag tcggtggttt ccaagccgat cgaggatctc
      661 gaggtcatgt tccgggggcc gccggcgatc catcgttacc ccgcctccat ggcgaagcgg
      721 gctcaggagc tggcgcatgc ggtgatcgac gactacgacg gcagggcgga gaatctctgg
      781 gataccgccg aggacggagc ggatctgctg agccggctgc gagcgcttcc cgggttcggg
      841 gcggagaagt cgaggatctt cgtcggactc ctcggcaagc gcctcggggt ccggcccgag
      901 ggctgggagg aaaaggcagc agactggcct tcgatagccg acgttgccaa cttcgaggac
      961 gtgctggcgc tgcgcgagaa gaagaaggcg atgaaggcgg ccaagaaagc ctgaacggct
     1021 caaccgccca ccgtttcctg ccgatagggg ggagcaaggg aacggaaggg tatcgatgca
     1081 tctctcgggc cagctctctg attggagcat caacgatctg ctccagatca tgcaggtcac
     1141 caagaagacc gggtcactcg acatcgaggg acagcgtcgc gggcgtgttc atttccgcga
     1201 ggggcgggtc acaggtgcgg aactgaccgg gttcgaagga gcacacgcag gatccgacct
     1261 cgccagtgtt gccgacattc tctacgtgct ctcgacggtc gccgagggct cgttctcggt
     1321 aggcgcggcc gatggccccg atgccaaagg ctggacggtg gaagaggtgc tcgtcgatgt
     1381 cgagtcattg caaaccctcg agggagaagt agccgaaacg ggtctcttcg aggcgaacgg
     1441 tgtaaagctg atcaacgaga tcggcgaatc ggtgacactg gatcccactg actggcagac
     1501 cttcgccaat ctggtgccgg cgttcagttt cggcgccctg gaggcgaggt acgggcgcgg
     1561 aaccgcggtc cgcatgttcc ataccctgag tcggatgggg ctggtcgagg ccgtcgccat
     1621 tcccgaggaa gaggcggagt ggctcgatcg tctggccgag ggcgtcgctg cgaacagctc
     1681 ttggaacggg tccgacggag aaaccgaagt cgacactgcc gccgagccgg agctcgagat
     1741 cgacgaagag ccgatcgaac cggcagcagt cgaggagccg gagatcaccg aacccgagcc
     1801 gatttcggtg tcgatttcaa aacctggtcg ggccaacgtc gagctcaagg gtgtttccgc
     1861 accggcttcg acgaccttga ccgatggcgt ttacgacgag atacgacggc ttcggagccg
     1921 ggtcggcgag aagtaacgtc ggcgggcatg cctgcccgca cgaacctcgg gtctgatctc
     1981 cccttgatcg tggggattgc gggcgggtcc gggtcgggca agaccaccat cgccgaatcg
     2041 ctggtcgaga agttgaacgg acatgtcgcc ctcgtccagc acgatgcgta ttaccgccac
     2101 acgccggagc tgtcgttcga ggccaggtca caggtcaact acgaccaccc tgcgtcgttg
     2161 ga
//
LOCUS       DASTXO010000311         3008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_240024, whole genome shotgun sequence.
ACCESSION   DASTXO010000311 DASTXO010000000
VERSION     DASTXO010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240024"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1464)
                     /gene="nuoG"
                     /locus_tag="VFU96_06690"
     CDS             complement(<1..1464)
                     /gene="nuoG"
                     /locus_tag="VFU96_06690"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEU5112990.1"
                     /translation="MTDQAAPELVTITIDGVAHEVAAGSLLIKAAQDKGTYIPRFCWH
                     ERMNPVGMCRMCLVEVQGPRGPMLVTACTNNVADGMVVDTRNDTVKKAQEGVLEFLLA
                     NHPLDCPVCDKGGECPLQDQAFSHGPGESRFVEIKRNYEKPIAISELVLLDRERCILC
                     ARCTRFADEISGDPLIEFKSRANVTEVNTFPDLPFSSYFSGNTIQLCPVGALTAAQYR
                     FRARPWDLQMVESSGLHDTAHPRLSVQVSQNEILRLVGVDNDATNHGWLSDKERFGFQ
                     YLGSEERLVSPLVRRDGELVETTWADALDRVAERIGLIREEDGGGAFAVIGGAHGTNE
                     DAYALSKFARVVLGTNNVDVRLDDALASHFLAATVDRALISDIDTADTIVVWGPDLKE
                     EHPTLYLRVRRAAQELGVRLVVVHPRATGLDDRASLKLGYRPGGGFTLLDEIADGKHP
                     EVRQALSAERVVAIVGQAAFADGPKLAESVAAYVRNKA"
     gene            complement(1461..2774)
                     /gene="nuoF"
                     /locus_tag="VFU96_06695"
     CDS             complement(1461..2774)
                     /gene="nuoF"
                     /locus_tag="VFU96_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEU5112991.1"
                     /translation="MTTPLVVTKRMTEFPADSHTIDRYEATGGYEQARRATGMTRDEL
                     VEEIKASGLLGRGGAAFSAGLKWSFLAPARPSYLVINADESEPGTFKDRQLLERDPHQ
                     MLEGVIITAIANEVHQAFIYIRGEYPKPARRVQAAVDEAYARGYLGKGILGSEYDLEV
                     TVHLGAGAYICGEETALINSLEGLRGEPRLKPPYFPAAKGLYMQPTIVNNVETVSDLP
                     HILAMGAEKFIELGHPLDTGTFMLSVSGHVGKPGNYELPHGITWRELIFEVAGGIPNQ
                     RELKAIIPGGASSPWFVPEIHLDHQITKDMCAEAGSMLGSGAVIVMDDTTCMVRAAER
                     IVRFFAHESCGQCTPCREGTTWMEMILRRIEEGQGRSIDLDLLLDVSDNISVGLGWPP
                     KMTTICPLGPSAVSPVLALRDYFRDEVDAHVELGGCPLKRVEAHA"
     gene            complement(2771..>3008)
                     /locus_tag="VFU96_06700"
     CDS             complement(2771..>3008)
                     /locus_tag="VFU96_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112992.1"
                     /translation="APAVQVNYELIEGVTPARARELVRWLDSARPEVINTDEMQELFG
                     GVRSFDWAIREESGAVGPYPAFQPLGTVGSEGNA"
BASE COUNT          509 a          983 c          984 g          532 t
ORIGIN      
        1 ggctttgttg cgcacgtacg ccgcaaccga ctcggcgagc ttgggaccgt cggcaaacgc
       61 ggcctggccg acgatggcca ccacacgttc ggcgctcagc gcctgtctca cttcggggtg
      121 tttgccatcg gcaatctcgt cgaggagcgt gaagccgcct ccaggccgat agccgagctt
      181 caacgaggcc cgatcgtcga gaccggtggc acgggggtgg accacgacga gccggacacc
      241 gagctcctgt gccgcgcggc ggactcggag atagagggtg ggatgctcct ctttgagatc
      301 aggcccccag accacgatcg tgtcggcggt gtcgatatcg gagatgaggg cgcggtcgac
      361 ggtcgccgcc aggaaatgcg aggcgagggc gtcgtcgagg cggacgtcga cattgttggt
      421 cccgagcacc accctggcga acttggacag tgcgtaggcg tcctcgttgg tgccatgggc
      481 cccgccgatc acagcaaacg caccgccccc atcctcctcc cggatgagcc cgatcctctc
      541 ggccacccga tcgagggcat ccgcccaggt cgtctcgacc aactctccgt ctcgtcgcac
      601 cagcggcgaa acgagacgtt cctccgagcc gaggtactgg aagccgaagc gctccttgtc
      661 cgacagccat ccgtgattcg tggcgtcgtt gtccaccccc accaggcgaa ggatctcgtt
      721 ctggctgacc tggaccgaga gccgcgggtg cgcggtgtcg tgaagccccg agctctcaac
      781 catctgcagg tcccaggggc gggcgcgaaa ccgatactgg gccgcggtga gggcgcccac
      841 cgggcacagc tggatcgtat tcccggagaa gtaggaggaa aaggggaggt cagggaaggt
      901 gttgacctcg gtgacattgg ccctcgattt gaattcgatg agcgggtccc cacttatctc
      961 gtcggcgaag cgcgtgcatc gggcgcagag gatgcatctc tcgcggtcga ggaggaccag
     1021 ctcggagatc gcaatcggct tctcataatt gcgcttgatc tccacgaaac ggctctcccc
     1081 cgggccgtgg gagaaggcct ggtcctgaag cgggcactca ccgcccttgt cgcacaccgg
     1141 gcaatcgagg gggtggttgg cgagcaggaa ctccaggact ccctcctggg ccttcttcac
     1201 cgtgtcgttc ctggtgtcga ccaccatgcc atcggcgacg ttgttggtgc acgccgtgac
     1261 cagcatcggg ccgcgcgggc cctgcacctc caccaggcac atccggcaca tccccaccgg
     1321 gttcatccgc tcgtgccagc agaaccgggg tatgtaggtg cctttgtcct gggcggcctt
     1381 gatcagcagc gagccggcag ctacctcatg ggcgacaccg tcgattgtga tcgtgaccag
     1441 ctcaggagca gcctgatcgg tcacgcgtgc gcctcgaccc tcttcaaggg acaaccgccc
     1501 aactccacgt gggcgtccac ctcgtcccgg aagtagtcgc ggagggcgag gaccggtgag
     1561 acggctgatg gccccagcgg gcagatggtg gtcatcttcg ggggccatcc caggccgaca
     1621 ctgatgttgt ccgagacgtc gagcaggagg tcgaggtcga tcgatcggcc ctgtccctcc
     1681 tcgatgcgcc gcaggatcat ctccatccac gtggtgcctt cccggcacgg cgtgcattgc
     1741 ccgcatgact cgtgggcgaa gaagcggacg attcgctccg cggcgcggac catgcaggtg
     1801 gtgtcgtcca tgacgatcac cgcccccgat ccgagcatcg aacccgcctc ggcgcacatg
     1861 tccttggtga tctgatgatc gagatggatc tcggggacga accacggcga cgaggcgccg
     1921 ccggggatga tcgccttcaa ctcccgttga ttcggtatgc ctccggcgac ctcgaagatg
     1981 agctcccgcc acgtgatccc gtgaggcagc tcgtagttcc cgggcttgcc gacatggcca
     2041 gacaccgaga gcatgaacgt gccggtgtcg agaggatggc cgagctcgat gaacttctcc
     2101 gcccccatcg ccaggatgtg gggcagatcg gagaccgtct ccacattgtt gacgatggtg
     2161 ggctgcatgt agaggccctt ggctgcgggg aaatagggcg gtttgagccg cggctcgccg
     2221 cgcaggccct cgaggctgtt gatgagggcg gtctcctcgc cacagatgta ggcccccgcc
     2281 ccgaggtgca cggtcacctc caggtcgtat tcggagccga ggatgccctt gcccagatac
     2341 ccccttgcgt atgcctcgtc gaccgcagcc tgcacccgac gcgccggctt ggggtactcg
     2401 cctctgatgt agatgaaggc ttggtgcact tcgttggcga tggcagtgat gatgacgccc
     2461 tccagcatct gatgggggtc gcgctcgagc agctggcggt ccttgaaggt cccgggctcc
     2521 gattcatctg cgttgatgac cagataagaa gggcgggcgg gggcaagaaa gctccatttg
     2581 aggccggccg agaaggcagc accgccccgg cccaacaaac ccgacgcctt gatctcctcg
     2641 accagctcat ctcgggtcat cccggtggcg cggcgggcct gctcgtagcc gccggtcgcc
     2701 tcgtagcggt cgatggtgtg cgagtcggca gggaactcgg tcatccgctt ggtgacaacg
     2761 aggggtgtcg tcaagcgttg ccctcgctgc ccaccgttcc caagggctga aaagccggat
     2821 acggccccac cgctccggat tcctccctga tggcccaatc gaagcttcga accccgccga
     2881 acagctcttg catctcgtcg gtgttgatca cctcgggccg ggcgctgtcg agccaccgca
     2941 ccagctcccg ggcgcgggcc ggagtgacgc cttcgatcag ctcatagttg acctggactg
     3001 ccggcgcc
//
LOCUS       DASTXO010000312         3851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_756189, whole genome shotgun sequence.
ACCESSION   DASTXO010000312 DASTXO010000000
VERSION     DASTXO010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756189"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="VFU96_06705"
     CDS             complement(<1..979)
                     /locus_tag="VFU96_06705"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEU5112993.1"
                     /translation="MELFETWALTLIVFLPLAGALLIGIIPKTNETALKGTGLLVAVA
                     AFVLSLGVIAQFDFSGLPYNTYQFEVDVPWIGAINANYHLGVDGISLPLLVLSTVVMV
                     LAVIYSWNHWEEPHNPKAFLILMLILATGLNGTFLALDLVLFFIFFEVVLLPMYFMIG
                     IWGDRSPRKIPGFGREVQTRLYAAIKFFLFTLFGSAFMLLGFLALYFQSGESGERTFS
                     IPELTDLGASGAFTGRFAFLVFLAMFLGFAVKVPMWPLHTWLPDAHTAAPTVGSVLLA
                     AIMLKLGSYGFIRISLPILPEEAASWAPAIAILAVIGIIYGSLACLAQTD"
     gene            complement(995..3013)
                     /gene="nuoL"
                     /locus_tag="VFU96_06710"
     CDS             complement(995..3013)
                     /gene="nuoL"
                     /locus_tag="VFU96_06710"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEU5112994.1"
                     /translation="MIATEAEHAVEAGVTAGFWVDTAGIMLVLPLLAFLLILAVGKRM
                     KYHGAELAIGALAINSLWATVLFVMNMTDGVLAQRTFEIGRIGSDLVFELGWVVDGLS
                     IMMYFLVNVVGLIVFIYAVGYMHGDRRVDWFFAAFSLFAGSMLILVGAPNLIQLIIGW
                     EGVGLASYFLIGFYWEDMANVNAGNKAFMVNKVADIGLILGAIILGLSIGTFDFFEMS
                     TAAFDHSEPLGAVAVVGGALLFFGAMGKSAQFPLHIWLPDAMAGPTPVSALMHAATMV
                     TAGVYLMGRVFPLFEYLASDIRPWMVAIGAITLFAIGLLALVADDIKKVLAYSTVSQL
                     GYMMTAVAAGGYTAGLFHLFTHAFFKALLFLGAGAVIHAVHSNNMSDMGGLRKTMPRT
                     YWTFVVGSLSLAGILPLAGFWSKDEILVALGNEGYTAVMWIGIAGAFVTAFYMTRAVA
                     LTFHGEYKGHGHPHEAPGIMTVPLVALAIPSILFGFFNVPGWEIPGIGNFSEWLAVRV
                     VPMGDEHSEAIDLVLAGSGLFAALLGIALGWMVWGKDRATQAERDRFEVPALYPLLRN
                     KYYVDDLADGITAGTMGPLARFVNWVNDYVIDSIVNGVAFLTKGLGSFVYGVVDQRGV
                     DGVIHGLGAGADGAGSALRKMQTGRIQQYAASFVAGALALIILFVFVI"
     gene            complement(3027..3332)
                     /gene="nuoK"
                     /locus_tag="VFU96_06715"
     CDS             complement(3027..3332)
                     /gene="nuoK"
                     /locus_tag="VFU96_06715"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009343500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEU5112995.1"
                     /translation="MLLNQFLILAAAMFSLGIYGLLVRRNIVLILLSVELMLAAVNIN
                     LVAFEAVLRTPEAIGQVFTIFVITVAAAEVGIGLAIVLMIFRNRKSANVDELDLMRW"
     gene            complement(3332..>3851)
                     /locus_tag="VFU96_06720"
     CDS             complement(3332..>3851)
                     /locus_tag="VFU96_06720"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEU5112996.1"
                     /translation="NWVFLVVGLLTLLSAVKVVTAKNVVHAALFLVAALGGSAILFLM
                     LSAEFVAWVVVLVYIGAVLVLFLFGIMITRAPTGKDVGLDSPNKVWPAILSGVTFLLL
                     GWASLDAFGTTEIVDLGEATSTEVLGLSLLGRFVIPFEVVSFVLLAALIGGITIARRD
                     LSPLEEEQRGLV"
BASE COUNT          876 a         1198 c         1208 g          569 t
ORIGIN      
        1 tgtcggtctg agccagacag gccaacgagc cgtagatgat tccgatcacg gccaggatgg
       61 ctatggccgg ggcccagctg gcggcctcct cgggaaggat ggggagggag atcctgatga
      121 agccgtagga ccccagcttc agcatgatcg cggcgaggag caccgacccc accgtggggg
      181 cggcagtgtg ggcgtcagga agccaggtgt ggagcggcca catcgggacc ttgaccgcga
      241 agccgagaaa catggcgagg aagaccagga aggcgaacct cccggtgaac gctccggagg
      301 cgcctaggtc ggtcaactcc gggatagaga aggtgcgctc ccccgattca cccgactgga
      361 agtagagggc gaggaatccc aacagcatga atgcagaccc gaacagggtg aagaggaaaa
      421 acttgatggc ggcatagaga cgggtctgca cctcgcgtcc gaacccgggg atcttccggg
      481 gtgaccgatc accccagatc ccgatcatga agtacatcgg gagcagcacc acctcgaaga
      541 agatgaagaa cagcaccagg tcgagggcga ggaaggtgcc gttgagcccg gtggccagga
      601 tcagcatcag gatcaggaac gccttggggt tgtgaggttc ctcccagtgg ttccagctgt
      661 agatgaccgc caggaccatc acgacggtgg aaagaaccag aagaggcagg ctgatcccgt
      721 ccactcccag gtgatagttg gcgttgatcg ccccgatcca gggcacatcg acctcgaact
      781 ggtacgtgtt gtaggggagc ccggagaaat cgaactgtgc gatgacccca agggaaagga
      841 caaacgcggc cacggcgacc agcaaccccg ttcccttcaa ggcggtctcg ttcgtcttcg
      901 ggatgatgcc gatgagcagc gctcccgcca ggggcaagaa cacgatgagg gtgagggccc
      961 aggtttcgaa cagttccatt tgctctcagc tctcctatat cacgaaaacg aacaggatga
     1021 tcagggccaa tgcgcccgca acaaaagacg ccgcgtactg ctggatccgg cccgtctgca
     1081 tcttgcgcag cgccgacccg gcgccatcgg ctcccgcgcc cagaccatgg atcacaccgt
     1141 ccacgccgcg ctgatcgacc acgccataca cgaacgagcc gaggcccttg gtgaggaatg
     1201 cgacaccgtt gacgatcgag tcgatcacat agtcgttgac ccagttgacg aacctggcga
     1261 gcgggcccat cgtccccgcc gtgatgccgt cggcgaggtc gtccacgtag tacttgtttc
     1321 gcaacagcgg atataaggct gggacctcga atcgatcgcg ctctgcctgg gtggcccggt
     1381 ccttgcccca gaccatccat cccagggcga tgccgagcaa tgccgcgaac aacccgctcc
     1441 ctgccagcac gagatcgatc gcctcggagt gctcgtcgcc catcgggacc acgcggacag
     1501 ccagccactc gctgaagttc ccgatgcccg ggatctccca gccaggcacg ttgaagaaac
     1561 caaagagaat cgacgggatg gcgagtgcga ccaagggaac ggtcatgatg cccggcgcct
     1621 cgtgagggtg gccgtggccc ttgtactcgc cgtggaaggt gagcgccacc gccctggtca
     1681 tgtagaaggc ggtgacgaag gcaccggcga tcccgatcca catgacggcg gtgtatccct
     1741 cgttgccgag ggcgacgagg atctcgtctt tggaccagaa cccggcgaga ggcaggatcc
     1801 ccgccagcga cagggagccg acgacgaagg tccagtaggt ccgcggcatc gtcttgcgca
     1861 gcccacccat gtcgctcatg ttgttggagt gcacggcatg gatcaccgcc ccagcgccga
     1921 ggaagagaag tgccttgaag aaggcatggg tgaagaggtg gaagaggccc gccgtgtacc
     1981 cgccggccgc caccgccgtc atcatgtacc cgagctggga cacggtcgag taggcgagga
     2041 ctttcttgat gtcatcagcg acgagggcga gcagaccgat ggcgaagaga gtgatcgccc
     2101 cgatggccac catccagggc cggatgtccg aggcgagata ctcgaacaag gggaacaccc
     2161 gtcccatcag atagaccccg gccgtcacca tcgtggccgc gtgcatcagt gccgacaccg
     2221 gcgtcggccc ggccatcgca tcggggagcc agatgtgaag cggaaattgg gcgctcttgc
     2281 ccatcgcacc gaagaagagg agagcaccac cgacgacggc gactgccccc aagggctcgg
     2341 agtgatcgaa cgcagccgtg ctcatctcga agaaatcgaa ggtcccgatg gacaggccga
     2401 ggatgattgc tcccaggatc aacccgatgt cggccacctt gttcaccatg aacgccttgt
     2461 tgcccgcgtt cacgttggcc atgtcctccc agtagaaccc gatgaggaaa taggaagcga
     2521 ggccgactcc ttcccacccg atgatcagct ggatcagatt cggggcgcca accaggatca
     2581 gcatcgaccc cgcaaagaga gagaaggcag cgaagaacca gtcgactctg cggtcgccat
     2641 gcatgtaccc gaccgcgtag atgaacacga tcagcccgac cacgttgacc aggaagtaca
     2701 tcatgatcga cagcccgtcg accacccatc ccaactcgaa gacgaggtcg ctcccgatcc
     2761 ggccgatctc gaaggtcctt tgggcgagga ctccatcggt catgttcatc acgaacagga
     2821 cggtcgccca gaggctgttg atcgcaaggg ccccgatggc gagctccgcc ccgtgatact
     2881 tcatcctctt cccgacggcg aggatgagga ggaaggcgag caggggaagc accagcatga
     2941 tcccggcggt gtcgacccag aaccccgcgg tcacaccggc ttctaccgcg tgctccgcct
     3001 cggtggcaat cacaatcaca atccacctac caccgcatca gatcgagctc gtcgacattg
     3061 gccgacttcc ggttgcggaa gatcatcaag acgattgcga ggcctatccc aacctcggcg
     3121 gcagccactg tgatcacaaa gatcgtgaac acctggccaa tcgcctccgg agtccggagc
     3181 accgcctcga aggccacgag attgatgttc accgcggcga gcatcagttc gaccgagagc
     3241 aggatcagaa cgatgtttcg ccgcaccagc aacccgtaga taccgagcga gaacatcgcc
     3301 gccgccagga tcaggaactg gttgagcagc atcagaccaa tccccgctgc tcttcttcga
     3361 gggggctcag gtcgcgtcga gcgatggtga tgccaccgat gagggcggcg aggaggacga
     3421 aggacacgac ctcgaagggg atgacgaacc ggccgagcaa cgacagcccg agcacttcag
     3481 tcgaggtcgc ctcgccgaga tcgacgatct cggtggttcc gaaggcatcg agagacgccc
     3541 acccgagaag gaggaacgtt acgcccgaga ggatggctgg ccagaccttg ttgggcgagt
     3601 cgagcccgac gtccttcccg gtcggggccc gggtgatcat gatcccgaac aggaagagga
     3661 cgagaaccgc gccgatgtag accaacacca ccacccaggc gacgaactcg gcggagagca
     3721 tgaggaacaa gatggcggag ccgccgaggg cggcgacgag gaacagggcg gcatgcacga
     3781 cgttctttgc cgtcaccacc ttcaccgccg acagcaacgt cagcagaccg acaaccagga
     3841 acacccagtt g
//
LOCUS       DASTXO010000313         6658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1676965, whole genome shotgun sequence.
ACCESSION   DASTXO010000313 DASTXO010000000
VERSION     DASTXO010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6658
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676965"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VFU96_06725"
     CDS             <1..695
                     /locus_tag="VFU96_06725"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU5112997.1"
                     /translation="TDNNVRMAALVHLVRHAEVDNPGHLVYADLPGFGLSPHGVEQAR
                     RVGRYLGPRPVVAIWSSPLERSLRTAEEIAARTGVPVKVDPDLTEWAVIGRWKGHAWR
                     DIPSAFPGELEAYLEGPESFAFSGETLEELADRMSAVARRLDSEHPHGDVVIVSHQDP
                     IQAGILRLIGSPLSGLHQDKPGHGTVVTLRPGPAWRVETVWEPGDSPRFGEKRGLRVL
                     EHAGSPDGPTSA"
     gene            723..1469
                     /locus_tag="VFU96_06730"
     CDS             723..1469
                     /locus_tag="VFU96_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112998.1"
                     /translation="MTVPVLACTPLVAALLWALAMGVDSGPLAPGSVLLIVTGLLGLT
                     TVGMVGVTVTGGRWAHRTLLAAVAAMLVLAVVRPIDVLWGLALATTLIAAIAVFSPPL
                     VASLRRLPVAAELPTRAVLLPLLLIGIPFPLGISAWDETSPGTLIVGLSAPLTALWFA
                     RVFPGGLYAVRIIWPVLAVGLSFTQWWAPAIVSVVSGAAVALLAWHPSVRVAFHPPRE
                     VGTVYPIPPELAPREVLDSAELDEHGRPIQ"
     gene            1466..1717
                     /locus_tag="VFU96_06735"
     CDS             1466..1717
                     /locus_tag="VFU96_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5112999.1"
                     /translation="MRRRPNPWIAVPALGLGLIAGFLGWIVTDVSCRQPDGAGGFTTC
                     SGWAVLVAIVSFLVVTVGVALVLVLVYRSLAEWREKQQT"
     gene            complement(1748..2998)
                     /gene="fabF"
                     /locus_tag="VFU96_06740"
     CDS             complement(1748..2998)
                     /gene="fabF"
                     /locus_tag="VFU96_06740"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017719391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU5113000.1"
                     /translation="MPPERRRVAVTGLGAITPLGEDVGSTWDGMLSGQSGIGPISLFD
                     TDGYRTTIAGEVRGWEPEKHFDRREVRRLDRFAQFFLVAVRQSLQQAGLSFGEDDPAA
                     ARAGVVVGAGFGGMGSFIDEIVTLTERGPDRVSPTGVPRVIPNMAAGLASIEHNLLGP
                     VSCVVTACSASANAIGDGAELIRRGAADVVVAGGSEAAITTFGIATFAQARALSSRND
                     EPQRASRPFDAERDGFVMGEGGAALVLEDMDLAIERGAPVLAELTGYGMSADAYHITL
                     PRPGGSGAARAMVNAVEDAGLIPDDIGYINAHGTSTEANDSTETAAIRIAMGPVADSV
                     PVSSTKSMTGHILGGAGAVEAVACIMALRDQVLPPTINYEHPDPECDLDYVPNVKRPA
                     SLQHAMSNSFGFGGHNVSLIFSKV"
     gene            complement(3004..3249)
                     /gene="acpP"
                     /locus_tag="VFU96_06745"
     CDS             complement(3004..3249)
                     /gene="acpP"
                     /locus_tag="VFU96_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008287891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU5113001.1"
                     /translation="MERSEVAGRLSEVLVSELGLEESKISEEAHFEEDLDVDSLGVVE
                     LLMALEDEFGVKIPDEEAESIMTVGQAIDVVYGKLGG"
     gene            complement(3283..4032)
                     /gene="fabG"
                     /locus_tag="VFU96_06750"
     CDS             complement(3283..4032)
                     /gene="fabG"
                     /locus_tag="VFU96_06750"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEU5113002.1"
                     /translation="MSDKAEGRVALVTGGSRGIGRAIAHRLASAGHPVAVNYAQKPDA
                     AAEVVESIGRAGGKAVAYPADVSDGSQVEEMFAAIEADLGQVAVLVNNAGITADNLLF
                     RMSSDDFDRVIATNLKSVYFCTRLALRPMLRARWGRVVSIASVAGITGNAGQSNYAAS
                     KAGMIGFSKAVSKEVGSRGITVNVVAPGFIATDLTQDLSEAVKHEAVSRTALRRFGEA
                     DEVAGAVEFLVSDAASYISGQVLAVDGGLAL"
     gene            complement(4029..5120)
                     /locus_tag="VFU96_06755"
     CDS             complement(4029..5120)
                     /locus_tag="VFU96_06755"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEU5113003.1"
                     /translation="MTMRNAEITGWGKCMPPNVLTNHDLEQLVDTSDEWITTRTGIKE
                     RRISHVEASELAAVAGLRALAAAGKTVNDVDLLLVATCSGDSIIPSTASVVQKHMGAW
                     NAAAMDLNAACSGFVYGLVVGTNMIRGGTNNTVLLVGTEKLHFVTDFTDRTTCVLFGD
                     GAGAVVLEPTDEPIGLLSSELGMDGSVGHILAVPRDGSSGDPGPIDPRESAIGMEGPE
                     VFRRAVTTMGDSAARAIKDAGLSLEDVDLVIPHQANTRIIDATARRLGIDESKVFVNI
                     ESYGNTSAATIPVALTEALEQGRVNPGDNIVFTAFGGGLSWGSAVYRWGARVEPLGES
                     DAELPPADLNTMELLQANFDFFGLPEKLT"
     gene            complement(5125..6066)
                     /gene="fabD"
                     /locus_tag="VFU96_06760"
     CDS             complement(5125..6066)
                     /gene="fabD"
                     /locus_tag="VFU96_06760"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU5113004.1"
                     /translation="MSVGILFPGQGSQTVGMGADLFDAKDDLLGDRADEILGFSLRAL
                     CLDGPEEELTRTQHAQPALFAISFALWEMVVADGDLAPGGAAGHSLGEYTALAAAGVF
                     DFDTALRLVSLRGKAMARAADREDSGMAALIGVDLDQAREVTVARRELGGRLEVANVN
                     APGQVVVAGGSNDIEWLLESGSGLGIRRVVPLSVAGAFHSQFMIPAQEELTAALETIE
                     MSDPRFPVWSNTTARRHESREIADLLARQMVEPVLFSDSLLDMSAAGIDTFLHVGPGD
                     VTAGLARRTVRGAAVETISGVDDIRSALDALVTMDEH"
     gene            complement(6097..>6658)
                     /locus_tag="VFU96_06765"
     CDS             complement(6097..>6658)
                     /locus_tag="VFU96_06765"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEU5113005.1"
                     /translation="MEATSARARAHDLAIHIDGARIWNAVAATGIDGPAWAALADTIQ
                     FCFSKGLGAPVGSIVCGPADLISEIRYLRKRLGGGMRQAGVIAAAARVALADRDRLSE
                     DHVLARSLGDAIAERFPDALDASLVETNMVRVQFGALGIEWPEMKERLDGAGIKVNPP
                     LGGGWRIVTHRDVDSSDVERLLLILP"
BASE COUNT         1106 a         2272 c         2093 g         1187 t
ORIGIN      
        1 cgaccgacaa caatgtccgg atggctgcac tggtacatct ggttcgccac gccgaggtgg
       61 acaacccggg gcatttggtc tacgccgatc tccccgggtt cggactctcg ccccacggcg
      121 tggaacaggc gaggcgagtt ggccgctatt tgggcccgcg gccggtcgtg gccatctggt
      181 cctcccctct cgagcgctcc ctacgcaccg ctgaggagat cgcggcgcga accggagtcc
      241 cggtcaaggt cgacccggac ctcactgaat gggccgtcat cgggcgatgg aagggccatg
      301 cctggcggga cattccttca gcattcccgg gagagctgga ggcgtacctc gagggccctg
      361 agagctttgc cttctccggt gagaccctgg aggaattggc tgaccgaatg tcggccgtcg
      421 cacggaggct cgactctgag cacccccacg gtgatgtggt catcgtctcg caccaggacc
      481 ccatccaggc cgggatcctg cggctgatcg ggtcgccttt gagcgggctc catcaggaca
      541 agcccggtca cgggacggtg gtgacgcttc gccccgggcc ggcgtggcgg gtggaaacgg
      601 tctgggagcc gggtgactcc ccgcgcttcg gtgagaaacg agggcttcgg gtcctggagc
      661 acgccgggag ccccgacggc ccgacttctg cctgatcctc gtcgcctgcg aaactcgacc
      721 ccgtgacagt ccccgtgctg gcatgcacac cactcgtcgc ggcgctgctt tgggcccttg
      781 cgatgggagt ggatagcggt cccctcgccc cggggtcggt ccttctcatc gtcaccggtc
      841 tactcgggct gaccactgtg ggcatggtcg gagtgacagt gaccgggggt cgctgggccc
      901 accggacatt gctcgccgca gtcgcggcca tgttggtgct ggcagtggtg cgaccgatcg
      961 atgtgttgtg gggcctcgct ctcgccacca cgctcatcgc cgcgatcgcg gttttctccc
     1021 cgcccctcgt tgcctcgctg cggaggcttc ccgtcgcagc cgagctgccg acgcgtgctg
     1081 ttctcctccc cctcttgctg atcggcatcc ccttcccgct cggaatctca gcgtgggacg
     1141 agacgtctcc ggggacgctc atcgtggggc tgagcgcgcc gttgactgcg ttgtggttcg
     1201 cccgggtgtt ccctggcggg ctatacgcgg tccggatcat ttggcccgtg ttggccgtcg
     1261 ggctctcctt cacgcaatgg tgggccccgg cgatcgtttc tgtggtctcg ggggctgcgg
     1321 tggcactgct ggcatggcac ccgtcggtgc gagtcgcatt tcacccgcca cgtgaggtgg
     1381 gtacggtcta cccgatcccg cccgagcttg cccctcggga ggttctcgat tcggcggagc
     1441 tcgacgagca tgggcgcccg atccaatgag gagaagaccg aacccctgga tcgcggtccc
     1501 cgccctcggt ctcggcctga tagccggatt cctgggctgg atcgtcaccg atgtgagctg
     1561 tcgtcagccc gacggcgcgg gtggcttcac cacgtgttct ggctgggccg tcctcgtcgc
     1621 catcgtctct ttcctcgtgg tcacggtcgg cgtcgccctg gtgttggtcc tggtctatcg
     1681 ctcgctggcc gaatggagag agaagcaaca gacctgatcg ccggttggga tgtggaccgg
     1741 tgagagatca aaccttgctg aagatcaatg acacgttgtg cccgccaaag ccaaacgagt
     1801 tcgacatcgc atgctgcagt gacgccggcc gcttgacgtt cgggacatag tcgagatcac
     1861 attccggatc gggatgttcg taattgatgg tcgggggcaa cacctgatca cggagagcca
     1921 tgatgcaggc aaccgcctcc actgcccctg cgccgccgag gatgtggccg gtcatcgact
     1981 tcgtcgacga gacagggacg gaatcggcga caggccccat tgcgatacga atcgcggcgg
     2041 tctccgtcga gtcgttcgcc tctgtcgatg tgccgtgtgc attgatgtag ccgatgtcgt
     2101 ctgggatgag acccgcgtcc tcgacggcat tgaccattgc ccgcgccgca ccgctccccc
     2161 ccggacgggg caaggtgatg tgataggcat ctgccgacat cccatagccg gtcaactccg
     2221 caagcaccgg cgcaccacgt tcaatcgcca ggtccatgtc ctcgaggacg agcgctgcgc
     2281 caccctcacc catcacgaac ccatcacgtt cggcgtcgaa gggccgcgac gcccgctgtg
     2341 gctcgtcatt ccgactcgag agcgccctgg cctgagcgaa ggtggcgata ccgaatgtgg
     2401 tgatcgctgc ctccgagcca ccggcgacca cgacgtcagc cgccccgcgg cggatcaatt
     2461 ccgccccatc tccgatggcg tttgccgatg ccgaacacgc tgtcaccaca caagagaccg
     2521 gtccgagcag gttgtgctcg atcgaggcca gccccgccgc catgttgggg atgacccgtg
     2581 gcacgccggt cgggctgacc cggtccggcc ccctctcggt cagagtgacg atctcgtcga
     2641 tgaaggaccc catgccacca aatccggcgc caacgacgac accggctcgg gcggccgcag
     2701 gatcgtcctc gccgaaggac aacccagcct gctgcaggga ctggcgaacc gcgacgagga
     2761 agaactgggc gaatcggtcg agtctccgga cctcgcggcg atcgaagtgt ttctccggct
     2821 cccagccccg gacctcgccg gcgatggtcg tgcggtaccc gtcggtgtcg aagaggctga
     2881 tcgggccgat cccggactgg cccgagagca tgccgtccca ggtcgagccg acatcttctc
     2941 ccagcggagt gatcgccccg agcccggtga cggccacccg ccgtcgctcg ggtggcatct
     3001 agatcaaccc ccgagctttc cgtacaccac gtcgatggcc tggccgactg tcatgatcga
     3061 ctctgcctcc tcatcgggga tcttcacccc gaactcgtcc tccagggcca tcaagagctc
     3121 gaccactccc aggctgtcca cgtcgaggtc ctcctcgaag tgggcctctt cggagatctt
     3181 cgactcctcc aggccgagct ccgagacgag cacctcgctc agtcgaccgg ctacttcact
     3241 tctttccact gttcctcctt cctggaattg tttggcggaa agctacaagg cgaggccgcc
     3301 atcgacggcc aacacctgac ccgagatgta agaggcggcg tcggatacga ggaactcgac
     3361 cgccccggcc acctcgtcgg cctcaccgaa tcgtcgcagg gcggtacggc tcaccgcctc
     3421 gtgcttgacc gcctcgctca ggtcttgagt gaggtcggtg gcgatgaacc cgggggcgac
     3481 cacgttgacc gtgatccccc gcgatccgac ttctttcgaa accgccttgg agaacccgat
     3541 catccccgcc ttcgacgcgg cgtaattgga ttgtcccgca ttgccggtga tccccgccac
     3601 cgaggcgatc gacaccaccc ttccccaccg ggccctgagc atgggacgca aggcaagacg
     3661 cgtgcagaaa tagacggact tcaggttggt ggctatgacc cggtcgaagt catccgagct
     3721 cattctgaac aggaggttgt cggccgtgat ccccgcattg ttgaccagca ccgccacctg
     3781 gccgagatct gcctcgatcg cggcaaacat ctcctcgacc tgcgagccgt cggagacatc
     3841 tgcgggatag gcgacggctt tacccccggc ccggccgatc gactccacga cctcggccgc
     3901 ggcatcgggc ttctgtgcgt agttgaccgc caccgggtgg ccggctgagg cgagacgatg
     3961 ggcgatcgcc cgaccgatcc cccgcgaacc gcccgtgacc agggcgaccc tcccctcggc
     4021 cttatcactc acgtgagctt ttctgggagg ccgaagaagt cgaagttggc ctgaagcaac
     4081 tccatcgtgt tgaggtcggc agggggcaac tcggcatcac tctcgccaag gggctcgaca
     4141 cgagcacccc accggtacac cgccgagccc cagctgagcc cgccgccgaa tgccgtgaac
     4201 acgatgttgt cgcccggatt caccctgccc tgttccagtg cttcggtcag agcgaccggg
     4261 atggtcgccg cagacgtgtt gccatacgac tcgatgttga cgaacacctt gctctcatcg
     4321 atgccgaggc ggcgggcggt ggcatcgatg atcctcgtgt tggcctgatg ggggatgacg
     4381 aggtcgacat cctccagcga gaggccggcg tctttgatgg cacgggcagc ggagtccccc
     4441 atcgtggtga ccgctcgccg aaagacctcc ggcccttcca tgccgatcgc cgactcgcgg
     4501 ggatcgatcg ggcccgggtc gcctgagctg ccatccctgg gcacagcgag gatgtggccg
     4561 acagatccgt ccatccccag ttccgaggag agaagcccga tcggctcatc ggtcggctcc
     4621 aacaccacag ctccggcgcc atctccgaag aggacgcacg tggtcctgtc ggtgaagtct
     4681 gtcacgaaat ggagcttctc ggtcccgacc agcaggaccg tgttgttggt cccaccccgg
     4741 atcatgttgg tcccgaccac cagtccatag acgaacccgg agcatgcggc gttgaggtcc
     4801 attgcggccg cgttccaggc ccccatgtgc ttctgcacga ccgacgccgt ggacggaatg
     4861 atcgagtcac cggaacatgt cgcgaccagg agcaggtcga cgtcattcac cgtctttccg
     4921 gcagcggcca gcgcccggag ccccgcgacc gcggcgagct cggacgcctc gacgtggctg
     4981 attcgtcgct ccttgatccc ggttcttgtg gttatccatt catcggaggt gtcgacgagc
     5041 tgctcgagat cgtggttggt gagcacgttg gggggcatgc acttgcccca gcccgttatc
     5101 tcggcgttcc gcatggtgat ctcctcagtg ttcgtccatc gtaacgaggg cgtccaacgc
     5161 ggaccggatg tcgtcgacgc ctgaaattgt ctccaccgca gcaccccgga ccgttctgcg
     5221 ggcgagacct gcagtcacat cccccgggcc gacgtggaga aacgtgtcga tcccggcggc
     5281 cgacatgtcc agcagggagt cggagaagag gaccggctcg accatctgac gggcgaggag
     5341 gtcagcgatc tcccggctct cgtgccgtct agctgtcgtg ttcgaccaga ccgggaacct
     5401 ggggtcggac atctcgattg tctcgagggc cgccgtcaac tcctcctgtg cggggatcat
     5461 gaactggctg tgaaatgccc cggccacact gaggggcact actcgccgaa tcccgagccc
     5521 ggaaccgctc tcgagaagcc actcgatgtc gttgctccca cctgcgacca cgacctggcc
     5581 cggggcattg acattggcca cctccagcct gccacccagc tccctcctgg ccacggtcac
     5641 ctcccgcgcc tgatcaaggt cgacgccgat caaggccgcc atacccgagt cctcccggtc
     5701 tgcagcacgc gccatggctt ttcccctcaa tgacacgagg cggagagcgg tgtcgaaatc
     5761 gaacactccg gctgcagcca aagccgtgta ttcccccagc gagtgcccgg cagctccacc
     5821 gggagccagg tcgccatcgg caacgaccat ctcccagagg gcgaaggaaa tggcgaagag
     5881 cgcaggctgg gcatgctggg ttcgggtcaa ctcttcctcg ggcccgtcca ggcacagagc
     5941 tcgcagggag aaaccgagga tctcgtcggc gcgatcgccg agcaggtcgt ccttcgcatc
     6001 gaagaggtcg gcccccatgc ccacggtctg gcttccctga cccgggaaca ggattccgac
     6061 actcaccagg ttcagactcg ggccggccgt cccccgttac gggaggatca agagaagacg
     6121 ctccacgtct gaggagtcga cgtcgcggtg ggtgacgatc cgccacccgc cgccaagcgg
     6181 aggattgacc ttgatgccgg cgccgtccaa tcgctccttc atctcgggcc actcgatgcc
     6241 caatgcgccg aattgaaccc tgaccatgtt ggtctccacc aaagacgcgt ccagggcgtc
     6301 ggggaatcgc tcggcgatgg cgtcgccaag cgagcgagcc aggacatgat cctcgctgag
     6361 ccgatcacgg tcggccagag cgacacgggc cgcggcggca atcacacccg cctgacgcat
     6421 cccaccgccg agtctcttgc ggagataccg gatctcggaa atcaggtcgg ccgggccgca
     6481 gacgatcgat ccgaccggcg ccccgaggcc tttcgagaag cagaactgga tcgtgtcggc
     6541 gagggcggcc caggcagggc cgtcgatgcc cgtcgccgca accgcgttcc aaattcgggc
     6601 cccgtcgatg tggatagcga ggtcgtgcgc acgggcccgc gccgacgtcg cctccatg
//
LOCUS       DASTXO010000314         3191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1235060, whole genome shotgun sequence.
ACCESSION   DASTXO010000314 DASTXO010000000
VERSION     DASTXO010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235060"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VFU96_06770"
     CDS             complement(<1..563)
                     /locus_tag="VFU96_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5113006.1"
                     /translation="MDLVEQVREAVIGKDTAVMTPFGLRRVTYADYTASGRSLAMIED
                     FIRAQVLPLYANTHTETSGTGLQTTRFREEAREIIRRAVGATHDEHAVIFAGSGSTGA
                     VDRLVGLLGIRIPKGLSETFGLEDRIPEDERPVVFVGPYEHHSNELPWRESIAEVIEI
                     KEDADGHVDLVELETRLVEHSHRPLIIG"
     gene            581..1606
                     /locus_tag="VFU96_06775"
     CDS             581..1606
                     /locus_tag="VFU96_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009191582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEU5113007.1"
                     /translation="MIHKTRKTMGDETTVSVGGEEIRISSPGRVIFPDQGWTKLDVAN
                     HFALVSQGAIRGVFGRPTMLKRFMGTVDENPIYHKRAAKNTPFETVQIRFPSQRPGMM
                     NVPRTEGDVVRLVQLGCLDFHPWPVRAEDIDHPDELRFDFDPTPGFDFEKVKEAAAGM
                     RALLEEVGLVGWPKTSGSRGIHVYIRIEPLWDFFQTRRAVLAAGRELERRMDGLVTTK
                     WWKEERNGVFIDYNQNARDKSVCSAYGVRPTGYVSTPITWDELADVAIEDFPLGSFAT
                     RYEAVGDLTEGIDESAGRIDTLMEWVARDQENGLGDAPWPPHYPKMPDEPPRVQPSKQ
                     RRPSEDY"
     gene            complement(1634..2362)
                     /locus_tag="VFU96_06780"
     CDS             complement(1634..2362)
                     /locus_tag="VFU96_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113008.1"
                     /translation="MTLALHLYRRIMRRGRVIGLLALAAVPGLVFWLSAFDAPDDELI
                     TLYTDVLASVGYTFAIAALILTTATLRDERDSGTLPYIYMRPIARVNMAAQSIAAGTV
                     AALVIGVGGWIASAVALLAVGGDLQAAVPALALFVAAGFGYAAIFVPLGYLVPRALLV
                     GLGYILVVETILAFAVEGLAQISIWRISLSIYAGLEDGFGEVARQAMTPVTPGVWGGV
                     AKLAGVLVIGLVVLTWALRRRDAL"
     gene            complement(2370..>3191)
                     /locus_tag="VFU96_06785"
     CDS             complement(2370..>3191)
                     /locus_tag="VFU96_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5113009.1"
                     /translation="GLLGPNGAGKTTLLRVMAGLQRPSQGEVRILGVDPRQDTEIYRK
                     ISLVPEDESVYDRLTGRQFVELAARLAKIDHPVERAQTVLDTVGLTDAADRALGGFSK
                     GMRQRAKVGAALVSDPEILLLDEPLNGADPVQRAQLIGLFKQLGAEGKTLLVSSHVLT
                     EVERVSDRVVAMVDGRLAAVGDVATIRAAMTDKPRRIYVDTTNPRKLAAALIAGSDVL
                     GVQVDGGTVHVETTDAGDLARRLPAIAIDHSIPISKVEPVDESLESVFRYLVEGR"
BASE COUNT          576 a         1031 c         1061 g          523 t
ORIGIN      
        1 ccgatgatca gcggacgatg ggagtgctcg acgagcctcg tctccagttc gacgaggtct
       61 acgtgcccat cggcatcctc tttgatctcg atcacctcgg cgatcgactc ccgccacggg
      121 agctcgttcg aatggtgctc gtaaggccct acgaagacca cgggtcgctc gtcctcgggg
      181 atccggtcct cgaggccgaa cgtctcgctg aggccttttg ggatcctgat gccgagaaga
      241 cccaccaatc tgtcgacggc gcccgtggat ccgctcccgg cgaagatcac cgcgtgctcg
      301 tcgtgggtgg ctccgacggc tcgcctgatg atctcccgag cctcctctct gaaccgcgtt
      361 gtctgcagcc cggtaccgga cgtctcggtg tgggtgttgg catagagagg gaggacttgc
      421 gcccggatga agtcctcgat catggccagt gatcggcccg aggcggtgta gtcggcgtag
      481 gtgacccgac gcagcccgaa cggggtcatc actgcggtgt ccttgccgat caccgcctct
      541 ctcacctgct cgaccagatc catccttcgc ccttcccggg atgatccaca agactaggaa
      601 gacgatgggc gacgagacca ctgtcagtgt tggcggggag gagatcagga tctccagccc
      661 ggggcgggtc atcttccccg atcagggttg gacaaagctc gacgtcgcca atcacttcgc
      721 cctggtttcc caaggcgcga tcaggggagt cttcggtcgt ccgacgatgc tgaagcggtt
      781 catggggacc gtcgacgaga acccgatcta tcacaagcgc gccgccaaga acacgccgtt
      841 cgagacggtc cagatcagat tcccctcaca acggccgggg atgatgaatg tcccccggac
      901 ggagggcgac gtcgttcgcc tggtccagct cggctgtctc gactttcatc cgtggccggt
      961 ccgggcagag gacatcgatc atccagatga gctccgcttc gacttcgatc cgacgccggg
     1021 cttcgacttc gagaaggtga aggaggcggc ggcggggatg cgggcgctgc tcgaggaagt
     1081 gggcctggtt ggttggccca agacgtcggg gagccgtggc atccatgtct atatccggat
     1141 cgaaccgcta tgggacttct tccagacccg acgggcagtc ctggcggcgg gccgcgagct
     1201 ggagcggcgg atggacggcc tggtcaccac caagtggtgg aaagaggagc ggaatggggt
     1261 cttcatcgac tacaaccaga acgcccggga caaatcggtg tgctcggcgt atggcgtccg
     1321 ccccaccggg tacgtctcga cgccgatcac gtgggacgag ctggcggacg tcgcaatcga
     1381 ggacttcccc ctcggatcgt ttgccacgcg gtacgaggcg gttggtgacc tcaccgaggg
     1441 tatcgacgag tcggcgggtc ggatcgacac gctgatggag tgggtggccc gggatcaaga
     1501 gaacggtctc ggcgacgcgc cctggccgcc gcattatccc aagatgcccg acgagccgcc
     1561 ccgggtccag ccctccaaac agcgtcgacc gtccgaggac tattagggac caggggtccc
     1621 gtcggacaca cgtctagagc gcgtcccgtc tccgcagggc ccaggtgagg accacgaggc
     1681 caatgacgag cacgccggcc agcttggcca ccccgcccca gactccaggg gtcacgggtg
     1741 tcatcgcctg gcgggccact tcaccgaaac cgtcctcgag cccggcgtag atggagaggg
     1801 agatccgcca gatggagatc tgggccaggc cttccaccgc gaaggcgagg atcgtctcca
     1861 cgaccaggat gtagccgaga ccgacgagaa gggcgcgggg caccaggtat ccgagcggga
     1921 cgaatatggc ggcgtatccg aaacccgccg ccacgaacag ggcaagcgcc gggaccgccg
     1981 cctgcagatc gcctccgacg gccagcaggg ccacggcgga ggcaatccag ccaccgaccc
     2041 cgatcacgag ggcggcaacc gtcccggcag cgatcgattg tgcggccatg ttcaccctgg
     2101 cgatcggtcg catgtagatg tacggcagag tcccgctgtc gcgctcgtcg cgcagcgtgg
     2161 ccgtagtcag gatgagggcg gcgatggcga aggtgtaacc gaccgacgcc aggacgtcgg
     2221 tatagagcgt gatgagctcg tcgtcgggcg cgtcgaatgc ggaaagccag aacaccaggc
     2281 ccgggaccgc ggcaagggcg aggaggccga tgacccgtcc ccggcgcatg atccgtcggt
     2341 agagatggag ggccagggtc acgagaccac taccgaccct cgaccaggta gcgaaacacc
     2401 gattccagcg actcatcgac cggctcgacc ttgctgatgg ggatcgaatg atcgatcgcg
     2461 atcgcgggca gacggcgggc gaggtcgccg gcatccgtgg tctcgacgtg gaccgtcccc
     2521 ccgtccacct gaacgccgag gacgtcggac ccggcgatca aagccgcggc gagcttgcgc
     2581 gggttcgttg tgtcgacata gatccggcgg ggcttgtcgg tcattgcggc gcgaatcgtg
     2641 gcgacatcgc cgactgcggc cagacgccca tccaccatcg ccaccacccg gtctgacact
     2701 cgctccacct cggtcaagac atgtgatgac accagcaacg tctttccctc ggctccgagc
     2761 tgcttgaaga ggccgatcag ctgagcccgt tgcaccggat cggcaccatt gagaggctcg
     2821 tcgaggagga ggatctccgg gtcggagacg agggccgctc ccaccttggc ccgctgtctc
     2881 atccccttgg agaatcctcc cagcgcccga tcggcggcat cggtgaggcc gaccgtgtcg
     2941 agaacggtct gcgcccgctc caccggatga tcgatctttg ccagacgagc cgccagctcg
     3001 acgaactgtc tcccggtgag ccggtcgtac accgactcgt cctcgggcac cagggagatc
     3061 ttgcgataga tctcggtgtc ctgacggggg tcgactccca ggatcctcac ctcgccttgc
     3121 gacggccttt gcagaccggc catgactcgg aggagggtgg tcttgccggc gccgttgggc
     3181 ccgaggagcc c
//
LOCUS       DASTXO010000315         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_608845, whole genome shotgun sequence.
ACCESSION   DASTXO010000315 DASTXO010000000
VERSION     DASTXO010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608845"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="VFU96_06790"
     CDS             complement(<1..1161)
                     /locus_tag="VFU96_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEU5113010.1"
                     /translation="MATLSQFPPGFLWGVATASYQVEGAVAEDGRAPSIWDTFSHTPG
                     KTADGDTGDRAVDHYHRYAEDVALMAEIGVNAYRFSIAWSRLLPQGTGRVNQAGIDFY
                     RRLCKELADAGITPMATLYHWDLPQVLQDRGGWRNRESVDWFTEYAAVAKDALGDMVA
                     TWATLNEPWCVAFLGHSSGEHAPGINDTPGSLVVAHHLMLAHHSAIGVMRDTSPREDD
                     RLGIVLNLIPAWPQNETEEDRAAASAVDLVQNRLFADAVIHARYPEEVLALFERYGVS
                     DAIDVDELARARQPIDFLGVNYYNINHIEQVGGSESMRAWPGTWGARIVRPPGHLTEM
                     GWGVEPEGLTWMLERIGREHPGLPLVICENGAAYADEVGPDRSIDDPERTDYV"
     gene            complement(1184..>2103)
                     /locus_tag="VFU96_06795"
     CDS             complement(1184..>2103)
                     /locus_tag="VFU96_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU5113011.1"
                     /translation="EQMLSAFEGPILPQSAATFEYEGEQLDIYTVPMPDGTERELAML
                     QPGRQTSQFIDPADPSAGPIEWEGNWRLLEPAYALDPQWQNYSQAWDLMDFPLLLRNT
                     LVIAGLGTIGAVGSSILVAYGLARFHVPFGRSIMVVLIATIILPRYATLVPTYTIFLR
                     IGWVGTWLPLIVPHFFANAFNVFLLRQYFLTIPKELDEAATIDGAGPFRTMWSVLLPQ
                     MKAPIVAVSLFHFYFAWNDFFEPLIYLSARPELQPIAVGLRTFNTLYDDTPSLIQSGA
                     MLALIIPVMVFLFAQRIFLKGIDLSGVQK"
BASE COUNT          416 a          648 c          674 g          365 t
ORIGIN      
        1 gacatagtcg gtgcgttccg ggtcgtcgat agatctgtca ggacccacct cgtcggcata
       61 ggcagcgccg ttctcgcaga tcaccagagg caacccagga tgctccctcc cgatgcgttc
      121 cagcatccag gtcagaccct ccggctcgac gccccagccc atctcggtca gatgcccggg
      181 aggccgcacg atcctggccc cccaggtccc gggccatgcc ctcatcgatt ccgacccgcc
      241 aacctgctcg atgtggttga tgttgtagta attcaccccg aggaagtcga tcggctgccg
      301 ggctctggcg agctcatcca cgtctatcgc atcggacacc ccgtaccgct cgaacagcgc
      361 caggacctcc tcgggatagc gggcatggat gaccgcatcc gcgaacagcc ggttttgaac
      421 tagatcgaca gccgaagccg ccgcccgatc ctcctcggtt tcgttctggg gccaggcggg
      481 gatcagattc aggacgatgc cgagacggtc gtcttcccga ggtgaggtgt cccgcatcac
      541 tccgatcgcc gagtgatggg ccagcatcag gtgatgagca accacgagcg agcccggagt
      601 gtcgttgatc cccggggcgt gctcgcccga gctgtggccc aggaatgcaa cacaccaggg
      661 ctcgttgagg gtggcccaag tagcgaccat gtcgcccagg gcgtctttgg ccaccgctgc
      721 atactctgtg aaccagtcca ctgactcccg gttgcgccag ccgccgcggt cctgaagcac
      781 ctgaggcaga tcccagtgat acagggtcgc catcggggtg attcccgcgt ccgcaagttc
      841 cttgcacagc cggcgataga agtcgatgcc cgcctggttg acgcgaccgg tgccctgagg
      901 gaggagacgg gaccaggcga tcgagaagcg gtaggcattg accccgatct cggccatcag
      961 tgcgacgtcc tcggcataac ggtggtagtg gtccaccgcc cgatcgccgg tatcgccatc
     1021 ggctgtcttg cccggtgtgt ggctgaacgt gtcccagatc gacggagccc gcccgtcttc
     1081 ggccacggcg ccctcgacct gataggaagc ggtggccacc ccccacagga aaccaggggg
     1141 aaattgcgac aaagttgcca tctagtgctg cgtcctctct tccctatttc tggacgccgg
     1201 agagatcgat gcccttcagg aagatccgtt gggcgaagag gaacaccatc accgggatga
     1261 tcaacgccag catggcaccc gactggatca ggctgggcgt gtcgtcgtag agcgtgttga
     1321 aagtgcgcaa gcccactgcg atcggctgca attcgggtcg tgccgagagg tagatcaagg
     1381 gctcgaagaa atcattccag gcgaagtaga aatgaaaaag ggatacagcc acgatcgggg
     1441 ctttcatctg ggggaggagg accgaccaca tcgtgcggaa cgggccggcg ccatcgatgg
     1501 tcgctgcttc gtcgagctcc ttggggatgg tgaggaaata ctgtctgagc aggaacacgt
     1561 tgaaggcgtt ggcaaagaag tgggggacga tcaacggcag ccaagtgccc acccatccaa
     1621 tacggaggaa gatcgtgtag gtggggacaa gcgtggcgta acgagggagg atgatcgtgg
     1681 cgatgagcac caccatgatc gacctgccaa aaggcacgtg gaagcgggcc aacccatagg
     1741 cgaccaggat cgaggacccg accgccccga tcgtccccag tccggcgatg accagcgtgt
     1801 tcctcagcag gagcgggaag tccatcaagt cccaggcctg gctgtagttc tgccattgcg
     1861 ggtcgagcgc gtaagcgggc tcgagcaacc tccagttgcc ctcccattcg atgggcccag
     1921 ccgacggatc ggcggggtca atgaattgcg aggtctgccg ccccggctgg agcatcgcca
     1981 gctctcgttc cgtgccatcg ggcatgggaa cggtgtagat gtcgagctgc tcgccctcgt
     2041 actcgaatgt ggccgccgac tggggaagga tcggaccctc gaaggcggag agcatctgct
     2101 cgg
//
LOCUS       DASTXO010000316         2196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_240196, whole genome shotgun sequence.
ACCESSION   DASTXO010000316 DASTXO010000000
VERSION     DASTXO010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240196"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VFU96_06800"
     CDS             complement(<1..731)
                     /locus_tag="VFU96_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5113012.1"
                     /translation="MTDVLQPTDVALADERAGRFRIRGWWIAVIVGIIALQILVTSPT
                     VPALWDTYISGPVDQFSAWLRENRQIHPVFTGFFIPLSAAVDWGLTTVESFLKWLPWF
                     VLPLAVFLVIFRTGDWKMAMVATAAMLYPGLVGLWETTMETLSLMTIAVLIALAIGIP
                     LGVWAARLPRVDRAIRPVLDAMQTIPAPVYFIPIVLLFGIRRVPATIATVIYSLPPAV
                     RLTTLGIQGVPRQSVEAAEMYGSTGR"
     gene            complement(728..1750)
                     /locus_tag="VFU96_06805"
     CDS             complement(728..1750)
                     /locus_tag="VFU96_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017621443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEU5113013.1"
                     /translation="MTGDVCLSVEHIWKVFGAKPDKAIEMAGTGASREEVQQATGCTV
                     GVRDVSFTVGRGETFVVMGLSGSGKSTLIRCVSRLNDVTRGKVVLDGEDLTAMSDDQL
                     RQVRRHKMSMVFQHFGLFPHRRVIDNAAYGLEVQGIDKTERYERATKVLEVVGLKGWE
                     QSFPQQLSGGMQQRVGLARALTVDPEIMFFDEPFSALDPLIRRDMQDELIRLQETLQR
                     TIVFITHDFSEAIKLGDRIAIMKDGGFDQVGTATELISDPATDYVREFTKEIPKAKVL
                     TTAAVLKEGTGTPNGRTVQLDDTIETVLPALLDDDAEIWVEDPDGKQVGVVDRRAVAK
                     AIEPDK"
     gene            1833..>2196
                     /locus_tag="VFU96_06810"
     CDS             1833..>2196
                     /locus_tag="VFU96_06810"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU5113014.1"
                     /translation="MKKRIWILISAMALLLAACGDDGGTATTEAAGDGAATTEAADGG
                     TDTTAAAGGEGETINIVANAWTASALNAEIAAQLIEQELGNPVEIVAIDENTMFTGLS
                     DGSLDAVLEVWPSGVVAEE"
BASE COUNT          426 a          693 c          703 g          374 t
ORIGIN      
        1 cgcccggtgg agccgtacat ctccgccgcc tcgaccgatt gacgagggac cccctggatc
       61 cccagcgtcg tgagcctcac cgccggcggc agggagtaga tgacggtggc gatcgtggcc
      121 ggaacccggc ggattccaaa caagaggacg atcgggatga agtagacagg agccgggatg
      181 gtctgcattg cgtcgaggac cggcctgatg gcgcggtcca ctcgagggag acgcgccgcc
      241 cataccccga gggggatccc gatggcaagt gcgatcagga cggcgatggt catcaacgag
      301 agggtctcca tcgtggtttc ccagagacca accaacccgg gatagagcat ggcggctgtc
      361 gcgaccattg ccatcttcca gtccccggtc ctgaaaatga cgaggaacac agcgaggggg
      421 aggacgaacc agggcagcca tttgaggaag gactcgaccg tcgtgagacc ccaatcgacc
      481 gccgcagaca gcgggatgaa gaaaccggtg aaaacggggt ggatctggcg gttctcccgc
      541 aaccatgcgg agaattggtc caccgggccc gaaatgtagg tgtcccagag agcaggaacg
      601 gtgggcgagg tcaccaggat ctggagcgca atgatcccga cgatcaccgc aatccaccac
      661 ccccggatcc tgaatcgccc cgctcgctcg tcggcgagag cgacgtcagt gggctggagg
      721 acgtcggtca tttgtcgggc tcgatcgctt tggccactgc ccgacgatcc acgactccga
      781 cctgcttgcc gtccggatcc tcgacccaga tctcggcgtc gtcgtcgagc agtgccggga
      841 ggaccgtctc aatcgtgtcg tcgagctgaa ccgtccgacc attgggtgtg cctgtgccct
      901 ccttcagcac ggccgccgtg gtcagcacct tggccttggg gatctccttg gtgaattccc
      961 ggacgtaatc ggtggctgga tcactgatca gctccgtcgc ggtgcccacc tgatcgaacc
     1021 ctccgtcttt catgatcgcg atccggtcac cgagcttgat cgcctctgag aagtcgtggg
     1081 tgatgaagac gatggttcgc tgcagggtct cctgcagcct gatcagctcg tcctgcatgt
     1141 cgcgccgaat cagcgggtcg agtgcggaga acggctcgtc gaagaacatg atctccgggt
     1201 cgaccgtcaa cgcccgggcc agaccaaccc gctgctgcat cccgccggac aattgctggg
     1261 ggaagctctg ctcccaaccc ttgagaccaa cgacctcgag caccttggtc gcccgctcgt
     1321 aacgctcggt cttgtcgatg ccttgtacct cgagcccgta tgcggcgttg tcgatcaccc
     1381 gccggtgggg gaagaggccg aaatgctgga acaccatcga catcttgtgt cggcggacct
     1441 gacgcaattg atcgtcgctc atggcggtga ggtcctcgcc gtcgaggacg accttgcccc
     1501 gggtcacatc gttgagacgg gagacacaac ggatgagggt cgatttgccc gagcccgaca
     1561 agcccatgac gacgaaggtc tcgcctctgc ccactgtgaa tgagacatca cgcacgccga
     1621 ccgtgcagcc ggtggcctgc tgcacctctt cgcgggatgc gccggtcccg gccatctcga
     1681 ttgccttgtc gggcttagca ccaaagactt tccagatgtg ctcgaccgac aggcacacgt
     1741 cgccggtcac agccccttcg tgacagccac gtcggtaatg taccagcggc cccttccggc
     1801 cgcgatcgtc ggccggcgag cctaggagaa ccatgaaaaa gagaatctgg atactgatat
     1861 cggccatggc gttgctgctg gccgcatgtg gtgacgacgg cggcaccgcc acgacggagg
     1921 cagccggaga cggcgccgcc acgaccgagg cagcagacgg tggtaccgac accaccgcag
     1981 cagcaggtgg ggaaggtgaa accatcaaca tcgtggcgaa tgcgtggact gcatccgcgc
     2041 tcaatgcgga gatcgccgcc cagctgatcg agcaggaact gggcaacccg gtcgagatcg
     2101 tcgccatcga cgagaacacg atgttcaccg gtctctcgga cggctccctc gacgcagtgc
     2161 tcgaggtgtg gccttccggt gtggtggccg aggagc
//
LOCUS       DASTXO010000317         2275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_203424, whole genome shotgun sequence.
ACCESSION   DASTXO010000317 DASTXO010000000
VERSION     DASTXO010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203424"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..514)
                     /locus_tag="VFU96_06815"
     CDS             complement(23..514)
                     /locus_tag="VFU96_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113015.1"
                     /translation="MPQVRVSEHSDLAPELVLASARDFSGRRAEMWPDVHIEYLEIHE
                     VGEDYAEVTEGNPWPIGYVWERLHYDWSEPNVLRGRVVESNLFKPGSTWELWATPEDE
                     GSLVEIRAVRHLRGRGWLLAPFFLPFIPGSAADTVRGHLRHFLASIDRTNTEAREEKG
                     EAR"
     gene            complement(577..732)
                     /locus_tag="VFU96_06820"
     CDS             complement(577..732)
                     /locus_tag="VFU96_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113016.1"
                     /translation="MGRRVAFLGLAVFLALVLFASMTLSSSLDGCRDIMSRVVCEPGA
                     TLIVPPS"
     gene            877..1668
                     /locus_tag="VFU96_06825"
     CDS             877..1668
                     /locus_tag="VFU96_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113017.1"
                     /translation="MSSRLEDLRDLARMLDEGKISQGEYDIVKTEIIQAPAEEWEPDA
                     DPALPGVDEESPDEEPTAGWIGLVGQVPKTYRVAAAGALVVLVAGFVLGGRGDAAGSV
                     TVDPASLAAPMSSAAPDSLGFRLDQLAEGWNAVPDPPLITAGVMTTPEPGRLDSFLHR
                     FDRFSLVAGAYDPSDGSVYALMAQSKTDDASVSSLYVHLCYLLYPGSQGCLDTFIEET
                     GTFGKSHADVADIDHRANWVFEGHKWQLDIRDDLETIRVHTATSP"
     gene            complement(1674..>2275)
                     /locus_tag="VFU96_06830"
     CDS             complement(1674..>2275)
                     /locus_tag="VFU96_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016867483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEU5113018.1"
                     /translation="PEFRGAAQPATGISGRGRMSEAPVIHLTTDQLALGLPEILSSPQ
                     DDGVIEMLVVRPEENERVTPETVELTSVQGIDGDHWSKGKYREMPDIQIAIINSRLLD
                     LVSGDRRRWALAGDNIVADLDLSQENLVPGQKLRAGSAVLEITDVPHEGCNKFSARFG
                     AAALRFVNVGPGKELRLRGIYARVVQDGTVSVGDRIRKL"
BASE COUNT          425 a          725 c          707 g          418 t
ORIGIN      
        1 cagttgggca ggctagccag ggtcagcggg cctcaccctt ctcctcgcgg gcttcggtgt
       61 ttgtcctgtc gatgctggcc agaaagtgac gcaaatgacc ccgcaccgta tccgcggccg
      121 aaccgggtat gaaggggaga aagaaggggg ccagcaacca gccgcggcct cgtaggtgcc
      181 gcacggcccg gatttccacg aggcttcctt catcctcggg ggttgcccat agctcccaag
      241 tgctccccgg cttgaacagg ttggactcga cgacacgacc acgaagcacg tttggctccg
      301 accagtcgta gtgcagccgc tcccagacgt agccgatcgg ccacggattg ccctcggtca
      361 cttcggcgta gtcctcaccg acttcgtgta tttccaggta ttcgatatga acgtccggcc
      421 acatctccgc acgccgtccg gagaagtccc gcgccgacgc aagaacgagt tctggggcga
      481 gatcgctgtg ctcgctcacc ctgacctgtg gcatcacagg atcgtagaag ccgccgcgaa
      541 agcggtgctc agcactcgcg gtcgaatcca ggccggtcag ctggggggaa ctatgagagt
      601 tgctcccggc tcgcatacca cccgggacat gatgtcccga caaccatcca ggctgctgga
      661 aagggtcatc gaggcgaaca ggaccagagc caggaacacc gcgagcccca gaaacgccac
      721 tctcctcccc atcgacgttc attactagcg cgtcggcttt cattccaggc catcccggcc
      781 gcgtcattcc cggccgtcgg gtggggacta gtccgaatca cctatggatt tcgcctgcat
      841 ctgccgatgg atatgggttg ccgccaaggg agcagcgtga gcagccgact cgaagacctg
      901 cgagatctgg cccggatgct ggacgaaggc aagatctcac aaggcgagta cgacatcgtg
      961 aagaccgaga tcatccaggc tccggcggag gagtgggagc cggacgccga tccagccctt
     1021 cctggcgtgg acgaggagag cccggatgag gagccgaccg caggttggat tgggctcgtt
     1081 ggccaggtcc cgaagacgta tcgagtagcg gcggccggag ccctggtggt cctggtcgcg
     1141 gggttcgtcc tcggaggccg tggtgacgcc gccggcagcg tcacggtgga ccctgcatcg
     1201 ctggctgctc caatgtcgag cgcggcaccg gattcgttgg gatttcgtct tgaccaactg
     1261 gccgagggtt ggaacgccgt gcctgatcca ccgttgatca ccgcaggggt catgacgacg
     1321 ccggagccgg ggcggctcga cagcttcctt cacaggttcg accggttctc gctcgtcgcc
     1381 ggtgcctacg accccagtga cggctcggtc tatgccctca tggctcagtc caagaccgac
     1441 gacgcttcgg tgtccagcct gtacgtgcat ctctgctacc tcctgtatcc cggatcgcag
     1501 ggttgcctgg acacgttcat cgaggagacg gggacctttg ggaagagtca cgccgatgtc
     1561 gccgacatcg atcaccgtgc gaactgggta ttcgaaggac acaaatggca gctcgacatc
     1621 cgcgatgacc tcgagacgat cagggtccac acagccacca gcccttgacc gattcagagc
     1681 ttgcggatgc gatctccgac cgagacggtt ccatcctgca cgactcgggc atagatcccg
     1741 cgaagacgta actccttgcc agggccgaca ttgacgaagc ggagtgctgc ggcgccaaac
     1801 cgcgccgaga acttgttgca gccttcgtga ggcacgtcgg tgatctcgag gacggccgac
     1861 ccggccctca gtttctgacc agggaccaga ttttcctgac tcaggtcgag atccgcgacg
     1921 atgttgtcac cggccaacgc ccaacgccgg cgatcgccgg agaccagatc gagaagccgc
     1981 gagttgatga tcgcaatctg gatatcgggc atctctcgat acttgccttt cgaccagtga
     2041 tctccgtcga tcccttggac cgaggtcagc tccacggtct ccggcgtcac cctctcgttc
     2101 tcttctggtc ggacgaccag catctcgatg acgccatcat cctgagggct ggacaggatc
     2161 tcaggtaagc caagggcgag ctggtcggtg gtgagatgga tcacgggcgc ctcgctcatg
     2221 cggcctcggc cggagattcc ggttgccggc tgtgccgccc ctcgaaattc gggcc
//
LOCUS       DASTXO010000318         3284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1013671, whole genome shotgun sequence.
ACCESSION   DASTXO010000318 DASTXO010000000
VERSION     DASTXO010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013671"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2971
                     /locus_tag="VFU96_06835"
     CDS             <1..2971
                     /locus_tag="VFU96_06835"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5113019.1"
                     /translation="IGDAVMSVFGVPSAHEDDALRAVRAAWDLRTALVDLNDRLEERS
                     GVPIVIRTGVNTGLVVAGDSSAGQSFVTGDAVNVAARLEQAASPGEILIGDSTHRLVR
                     DSVRVEPLGALPVEGRDLPVTAFRLLDVSPEGVGLSRRLDSPLVGRQRELAVLDDVLN
                     RTDRERVCSLVSLIGSPGIGKSRLTGEFLAGAGDRARVVRGRCLPYGEGITFWPVTEV
                     VRDAAGIGEDDSPDQALARIEAILADEPEAALVAQRVAGVIGLAGGGAAIQEAYWAVR
                     KLLELLARDRPLIVVFDDIHWGQTAFLDLLEYLGGSAHQVRVMLICMARPDLLDARPS
                     WGTGQPNATTVHLEPLTEEESRQLISNLLAGAQVPEEALVRITQAAEGNPLFVEEMLR
                     MLVDDGLLRKEGPTWIAIGDLSSLSMPPSIQAVLASRLDRLLPEEQAAIQRASVVGKV
                     FWWGAVAELSPEEARPRIGTNLQSLVRKELIRPDPSEFAGEDAFRFGHILTRDAAYDS
                     IPKQIRGELHAQLADWLERMAGDRISEFEEIVGYHLEQAYRYQTELGAAGLRENALAE
                     RAGSVLGEAGFRAAQRGDSSAAENLLRRSVSLLPRADRRRLEIVPDLSLALMDLGQFR
                     EAEELLRDVDIDARKSGDRRAEWWSVTVRSLWQYLTHPAFWVRQAPHELKQALQVFDE
                     MGYERGLALAGFLLGSFHLLNGHAAESAAVAERGVEEARKADLPRDEARSLSLLAEAL
                     LVGPTPIEEGLERCASMIAKARGNQLLEAQVLLRAARLHAHLTKFDQARELNDRAKGI
                     LEELGLVSDVALAEWNGGVIEFLAGELGTAAMHLEIASRTFEGRGERGRSSRAGLALA
                     EAYYALGRFEDALAWAQFAERTGTPDDVEIMVRARLLRARLVARRGWLEDAEGRGREA
                     LILAERTDLLDLQTEALLGLADVLIAEGKSADAESLLESARSLCERKGNLVMLGRTRQ
                     LHANQTSSQSDGPRPVI"
BASE COUNT          575 a         1000 c         1117 g          592 t
ORIGIN      
        1 catcggagac gcggtcatgt ccgtctttgg cgtcccttca gctcacgagg acgacgccct
       61 tcgagccgtc cgagccgctt gggatctgcg cactgccctg gttgacctga acgaccgcct
      121 cgaagagcgc tcgggggtcc ctatcgtcat caggacgggg gtcaataccg ggctcgtggt
      181 ggcgggcgac tcctccgccg ggcagtcgtt cgtcacaggc gacgcggtga acgtggcagc
      241 caggttggag caagctgctt cacctggaga gatcctcatc ggcgatagca cgcatcgttt
      301 ggtacgggac tctgtccgcg tagagccctt gggagccctg cctgtcgagg gcagggacct
      361 tcccgtgacc gcttttcgcc ttctcgacgt ctcccccgag ggggtcggct tgagccggcg
      421 ccttgactca cccctggtcg gccgccaacg cgagctcgct gtcctggacg atgtcttgaa
      481 ccggacggat agggaacgcg tctgctcgct tgtttcactg atcggttcgc ccggtatcgg
      541 caagtcccgc ctgaccgggg agttccttgc tggggccggg gaccgggctc gggtggttcg
      601 agggcggtgc ctcccttacg gggaggggat caccttctgg ccggtcaccg aagtcgtgag
      661 ggacgccgct gggatcggcg aggacgactc ccctgatcaa gcactcgcga ggatcgaagc
      721 catcttggcc gacgagccgg aggcagcgct cgttgctcag cgcgttgcag gcgtgatcgg
      781 gctggcgggg ggcggggccg cgattcagga ggcctattgg gcggtgcgca agctgttgga
      841 gttgctcgct cgagaccgac ctctcatcgt cgtgttcgac gacattcact gggggcagac
      901 ggccttcctc gatctcctcg aataccttgg cggatcagca caccaagtcc gggtcatgct
      961 catctgcatg gcccggcctg acctactcga cgcgcgccct tcctggggta ccggccagcc
     1021 gaatgccacc acggtccacc tcgagccgct gacagaggaa gagagccgcc agctgatcag
     1081 caaccttctg gcgggcgccc aggtgcccga agaagccctc gttcgcatca cccaggcggc
     1141 cgaaggaaac cctttgttcg tcgaggagat gctccggatg ctcgtcgatg acggcttgtt
     1201 gcggaaggaa gggccgacgt ggatcgccat cggagacctc tcgagcctat ccatgccgcc
     1261 ttcgatccaa gctgtgcttg cctcgcgcct cgaccgactc ctccccgaag aacaggcggc
     1321 gatccagcga gcctcggtgg tgggaaaggt gttctggtgg ggtgcggtcg ccgagctgtc
     1381 gccggaagag gcccggccca ggatcggcac caatctccag agcctggtca ggaaggaact
     1441 tatccgcccg gacccctcgg agttcgcggg ggaggatgca ttcaggttcg ggcacatcct
     1501 cacacgagat gccgcctacg actcgatccc taagcagatc aggggggaac tgcacgcaca
     1561 gttggccgac tggctcgaac gaatggcggg agacaggatc agcgagttcg aggagatcgt
     1621 ggggtaccac ctcgagcagg cctaccggta tcaaaccgag cttggggcag ccggccttcg
     1681 tgaaaatgca ctagctgaac gggcggggag tgtgctcggc gaagccggtt tccgggctgc
     1741 ccaacgcgga gactccagtg ccgctgagaa cctgctccgg cgatccgtgt cgctccttcc
     1801 acgagccgac cgccgaaggc tggagatcgt cccggacctt tcgctcgctc tcatggatct
     1861 ggggcagttc cgggaggcgg aagagttgct cagggacgtc gacatcgacg cgaggaagag
     1921 cggcgatcgc cgggcggagt ggtggtcggt gaccgtccgt tccttgtggc aatacttgac
     1981 ccaccctgcc ttttgggtgc gtcaggctcc ccacgagttg aagcaggctc ttcaggtatt
     2041 cgacgaaatg gggtacgagc ggggactcgc gcttgccggg ttcctgctcg ggtcgttcca
     2101 tctcctgaac gggcacgcag ccgagtcggc ggcagtagcc gaacgtggag tggaggaggc
     2161 ccggaaggcg gatctacctc gtgacgaggc gaggtcgctg tctctcctgg cagaagcctt
     2221 gctggtcggg ccaaccccga tcgaagaggg cttggagcgg tgcgcatcca tgattgcgaa
     2281 ggcgcgaggg aaccaactgc tggaggctca agtgctcctc cgggctgccc gactccacgc
     2341 tcacttgacc aagttcgacc aggccaggga actcaatgac cgagcgaagg ggatcttgga
     2401 ggagctgggg ctcgtctccg acgtggccct ggctgagtgg aacggcggtg tcatcgagtt
     2461 cctggccggt gaactgggca cggctgcgat gcatcttgag atcgcatccc ggaccttcga
     2521 gggccgagga gagaggggcc gatcatccag agccgggctc gcattggcgg aggcctacta
     2581 tgcgctcggc agattcgagg acgccctggc ctgggcccaa ttcgctgagc ggaccggtac
     2641 gcccgatgac gtggagatca tggtcagggc gcgcctccta cgggcaaggc tcgtggcccg
     2701 gcggggttgg ttggaggacg cggagggtcg gggacgagag gctttgattc tcgcggagcg
     2761 aacagatctc ctcgacctcc agaccgaagc gttgctcggc ttggccgatg tcctgatcgc
     2821 cgaaggtaag tcagcagatg cggaatcgct tcttgagtcc gcgcgaagcc tctgtgagcg
     2881 gaagggcaat ctcgtcatgc tggggcgaac ccgacagctc catgcgaacc aaacctcgag
     2941 tcagtccgac gggccacgcc ccgtcatcta aggagcctcg ttcgaagacg agggatgagt
     3001 agccgccggt gacggtggag gcgaatccat cgcgtgtggc atttcggtta ctggccggtt
     3061 tcaggaagcc actggccagg ctagccccct ctaaacgctc gatccccagt gaagcgaacc
     3121 gcgtgtcatt gatcgagacg accgcgggca ccaacagggt gtgatcacgc ggagcgggat
     3181 ggctatcgat atcgatcggt gcccacaagt tgcgctcacc tatcggcgaa gaagctcttg
     3241 ctccacgacc aagaagacct cgtccagatc gagggccgtc cctt
//
LOCUS       DASTXO010000319         2674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_977036, whole genome shotgun sequence.
ACCESSION   DASTXO010000319 DASTXO010000000
VERSION     DASTXO010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977036"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..>2674
                     /locus_tag="VFU96_06840"
     CDS             43..>2674
                     /locus_tag="VFU96_06840"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5113020.1"
                     /translation="MSGQVPTENAVGPSFEVYLPMDRRRELAGLGTLPATSVGAALLA
                     DISGFTHLTEVLSRNLGPRRGAEELSRLLNQVFGPTTSAIHEHGGSVISFGGDSVISW
                     FAGDDGSQATAAALSLRAFIEVFREQEAGGPAGDIDIKVAVVHGTSRRVRVGRPEHAY
                     MDLLAGEVVESLSSEARLLQRGEVAVSREVAEALGFGAELRTISSDGHVRFLVDSLAS
                     EPDRVISIDDYSIDEAVTRQWLLPDVHERIEQRLQGLLAELRDVVAIFVGFEGIDHVN
                     DDDAGAALDTSVSWAQDVLAKYEGVVLQTLIDDKGCHLYAVFGASVSHEDEARRAVIA
                     ALELTEPSSESGAITAVRVGIAQGMAYVGSYGGPSRMTYGAHGPVVSLAARIMQQTPP
                     GMVYVTEQIASEPSTRVEFSEIGPTEFKGVDREVTIYRATAVDGHGAPGTPRRLSDGG
                     LVGRDTERGIVNARLAQLHGGQGGTILIEGTAGIGKSRLLRAFVEQAELLGTTVITTS
                     GEEIEQATAFYAWRPVLRQLFGGDGIGAERALLEFVAGDPWLEERKGLLAPIVSIGVE
                     DSNLIAAMEPELRGENTLRFLTEIIRAAPRASSPFVIVIDDGHWVDSASWAMAERAVR
                     DVPSLLLVVATRPFGEGSGRDTPDEYRHLLENEDTDHVLLSEMEPAEVMQLIENCLGV
                     RSVPGPVIEMIIEQAEGHPYFSEEIAFALRDAGLLVIENGESRLAPNVQDLRDVDFPS
                     TVQDIIIGRFDRLSARQQSVLKVASVIGREFSVDTLVEVYPADLDVSEAIESLQTLVV
                     LDLVHEVTLDRSRYRFRHAITRDIAYGLLLFSQKSQLHRAVALSMEAAHADDLDSVAT
                     TLAYHWRNSLSAESESGDVDK"
BASE COUNT          467 a          836 c          873 g          498 t
ORIGIN      
        1 ggggcacggg ctccacgtcg aatacactcg gaatgtgacg cgatgagcgg gcaagtgccg
       61 acagagaatg ccgtcggtcc atcgttcgag gtgtacctac ccatggatcg tcggcgtgag
      121 ctggccggcc tgggcacatt gcctgcgaca tctgtcggcg ccgcccttct cgccgacatc
      181 agcggattca cccacctcac cgaggtcctg agtcggaacc tcggcccgcg ccggggagcg
      241 gaagagctgt cccggctgct caatcaggtc ttcggcccga ccaccagtgc gatacacgaa
      301 catggtggga gcgtcatctc cttcggtggc gattccgtga tcagttggtt cgcgggggac
      361 gacggatccc aggccaccgc ggccgcgctc agcctgcgcg ccttcatcga ggtattccga
      421 gaacaggagg caggtgggcc tgccggtgac atcgacatca aggtcgccgt agtgcatggc
      481 acctcgcggc gggtgcgagt gggccgcccc gagcacgcct acatggatct gttggctggt
      541 gaggtcgtcg agtccctctc gtcagaggca aggttgctgc agagggggga ggtggccgtg
      601 agtcgggagg tggcggaggc gctcggattt ggggccgagc tgcgcaccat ctccagtgac
      661 gggcatgtcc gctttctggt ggattcgctg gcctccgagc cggatcgggt catctcgatc
      721 gacgattact ccatcgatga ggccgtcacc cgccagtggc tcctccccga cgtccacgag
      781 cgaatcgagc aacggcttca gggtctactc gcagagctac gggatgtcgt tgccattttc
      841 gtcggattcg agggcatcga tcatgtcaac gacgacgacg cgggagcggc cctcgataca
      901 tccgtctcct gggcgcagga cgtgctcgcc aagtatgagg gcgtagtcct ccagactctc
      961 atcgacgaca agggctgtca tctctatgca gttttcggcg cttcggtctc ccacgaggac
     1021 gaggcacgac gggcggtcat cgcagcattg gagctcactg agccttcctc cgaatccggc
     1081 gcaataaccg cggtccgggt cggaatcgcc cagggaatgg cctatgtcgg ctcgtatggc
     1141 ggtccgagtc gaatgaccta cggtgcccac ggccccgtgg tgagtctcgc tgcccggatc
     1201 atgcagcaga cgccacccgg gatggtctac gtgaccgaac agatcgcctc agaaccaagt
     1261 acgcgggtcg agttctcgga gatcgggccc actgagttca agggtgtgga ccgcgaggtg
     1321 accatctacc gcgctactgc cgtcgacggc cacggcgcac caggcacccc ccgtcgtctc
     1381 agcgacgggg gattggtcgg tcgggacacc gaacgcggca tcgtgaatgc ccgcctggct
     1441 caactccatg gaggccaggg gggcacgatt ctgatcgaag ggactgcggg catcggcaag
     1501 tcccgtctcc tcagggcttt cgtcgaacag gccgagttgc tcggcaccac ggtcatcacc
     1561 acctcgggtg aggagatcga acaggccacg gccttctacg cctggaggcc ggtgttgcgt
     1621 cagttgttcg gcggtgatgg gatcggggcc gaacgcgccc tcctcgagtt cgtcgccggc
     1681 gacccctggc tagaggagcg gaagggcctt ctcgccccga tcgtctccat cggtgtcgaa
     1741 gattccaact tgatcgccgc gatggagcct gagcttcgtg gtgagaacac cctgcgcttc
     1801 ctcaccgaga tcatccgagc ggctccccgc gcctcctccc cattcgtgat agtcatcgac
     1861 gacggccatt gggtcgactc cgcgtcctgg gccatggccg agagggcggt gcgcgacgtc
     1921 ccgtcattgc tgctcgtcgt ggccacccgg ccgtttgggg agggatcggg gcgcgacacg
     1981 cccgacgagt accgacatct gctcgaaaac gaggacacgg atcatgtcct cctctccgag
     2041 atggaaccag ccgaggtcat gcagttgatc gagaactgcc tgggtgtgag gagtgttccc
     2101 gggccggtga tcgagatgat catcgaacag gccgagggtc atccctactt cagcgaggag
     2161 atcgcgttcg cgctgcgcga tgccggcctg ctggtcatcg agaacggaga gagcagactc
     2221 gcacccaacg tccaggacct gcgtgacgtg gacttcccga gcaccgttca ggacatcatc
     2281 atcggtcgct tcgatcggtt gtcggcccgt cagcagtcgg tcctgaaggt ggcgagcgtg
     2341 atcggccgag agttctcggt cgacaccctg gtcgaggttt acccggccga cttggacgtg
     2401 agcgaagcca tcgagagcct gcagactctc gtggttctcg atctcgtgca tgaagtgacc
     2461 ctcgaccgat ccaggtatcg cttccggcat gccatcaccc gcgatatcgc gtacgggttg
     2521 cttctcttct cgcagaagag tcagctccat cgcgcagtag ccctatcgat ggaggcggct
     2581 cacgccgacg atctcgactc ggtggccacc acgctcgcct accactggcg taacagcctg
     2641 agtgcggagt cggagagtgg tgacgtcgat aaag
//
LOCUS       DASTXO010000320         3732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_645759, whole genome shotgun sequence.
ACCESSION   DASTXO010000320 DASTXO010000000
VERSION     DASTXO010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645759"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VFU96_06845"
     CDS             complement(<1..247)
                     /locus_tag="VFU96_06845"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU5113021.1"
                     /translation="MIRRYALGVGLAAFVVVVDAITKRWASIELVDGDIVIIPGFLVL
                     TFTENPGAAFSMFENAGPFFGIAALTVTAALLWSLRHD"
     gene            complement(244..615)
                     /locus_tag="VFU96_06850"
     CDS             complement(244..615)
                     /locus_tag="VFU96_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEU5113022.1"
                     /translation="MARQLAKSTVEKFQARLEEEQTRLQGLIDDYEQELEEARLSESS
                     SDRSPDPGNAEASSTKLEYAKELSIEQNTLDLLSKVERALEKIEQGTYGICESCGKSI
                     PLARLDVLPYATLCVDCARKA"
     gene            721..>3732
                     /gene="ileS"
                     /locus_tag="VFU96_06855"
     CDS             721..>3732
                     /gene="ileS"
                     /locus_tag="VFU96_06855"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU5113023.1"
                     /translation="MTNNEQDRPDSSAGFTRVEPTVDIPAMEQGIIDFWRETKAFEES
                     VLRRPADKEYIFYDGPPFPTGSPHYGNLLAGVIKDMVPRYWTMRGYRVDRRFGWDTHG
                     LPIEMEVEKRLGISGPKAIAEYGVDRFNEAARSVVQANTENWEVLTERIGRWVDFEND
                     YKTLDPEFMETVWWVFKQLWDKGLVYKDFKVLPYSWGAATPLSNFEANKDYRDVEDPS
                     ITVRLEVLESAGPMTAGDYILIWTTTPWTLPGNLAVAVGDEIAYSRITDGDDHYWIAT
                     ARAATVFGEDPNVVAEAKGSEILGVAYLPPFDYFSAEREAGAFRIISSPEVSVDEGTG
                     LVHMAPAYGEADFYALKGAGIDSLVDPVDAEGDFTDQVPEVAGQNVKVADETLIELLR
                     ASGSLVHSDRITHSYPFCERTDTPLIYKAIPTWFIRVEDIKDRMVEINDTIHWVPDYV
                     GSRRFGNWLVNARDWAVSRNRYWGSCIPVWECTGCDEAVAVGSLDELEALSGHRPADL
                     HKHVVDDIGWQCSSCGGSMIRVPEVLDGWFESGSMPYGMLHYPFENAERFEAAFPAKF
                     IAEGLDQTRGWFYTLIVLSTALFDAPAFENCVVNGLILAEDGRKMSKSLKNYPDPSEI
                     FDHMGADALRAFLINSPVLRAEPLRFSSDGVAEVVRTVLLPMWNTYSFFTTYAEADGM
                     TVADLGMAPPPADRPEMDRWILSVLQSLVSEVNTQMEGYYLYNVVPPTLGFIDHLTNW
                     YVRRSRRRFWRARGESGEGDTDKLAAFATLYEVLTRFIEVLAPVLPFMTERLYQDLVA
                     RHHPDAVPSVHHRDFPEAAIPLIDRDLEAAMEVVRDVVRLGRNLRKPEGLRVRQPLSK
                     LTVLTRSSSVALAVETHSDVIADELNVKEVATSSDDGSLVHLSAKANFRTLGPVFGPR
                     MPEAASAIADLGPADLEVVLDGGTVSIGGHDLGMGDLIIDRTPREETVVEAGSDLAVA
                     LDTHVSDELLLEGIARELISRVQRLRREASLD"
BASE COUNT          694 a         1186 c         1160 g          692 t
ORIGIN      
        1 gatcgtgacg aagggaccag agcaatgccg cagtcacagt cagggcagcg atgccgaaga
       61 aggggccggc attctcgaac atggagaacg ccgccccggg attctcggtg aaggtgagaa
      121 cgaggaagcc ggggatgatc acgatgtccc cgtcgacgag ctcgatcgaa gcccaccgct
      181 tcgtgattgc gtcgacgacg acgacgaacg cggccagccc gactccgagg gcgtacctgc
      241 ggatcaagcc ttgcgagcgc agtcgacgca gagcgttgcg tagggaagga cgtcgagtcg
      301 agccaggggt atcgacttgc cgcagctctc gcatatgccg taggtgcctt gttctatctt
      361 ctccagcgcc cgctcgacct tgctgaggag atcgagcgtg ttctgctcga tggagagctc
      421 cttggcatat tccagcttgg tcgagctggc ctccgcattg cctgggtctg ggctgcgatc
      481 ggaggagctc tccgagagcc tcgcctcttc cagttcctgc tcgtagtcgt cgatgagccc
      541 ctgcagccgc gtctgctctt cttccagcct tgcctggaat ttctccacgg ttgacttggc
      601 gagctgtcta gccatgagaa cccctgctaa agggcggaaa ccctaccaac gcatggcggc
      661 tatctggcga gtttcgccga agccggcaat ccgcggctcg ccctagcatt tccgactccc
      721 atgacgaaca acgagcagga cagaccggac tcgagcgccg gtttcacccg cgtcgagccg
      781 acggtggaca tacccgccat ggagcagggc atcatcgact tctggcgtga gacaaaggct
      841 ttcgaggaat cggtcctgcg acgtcctgcc gacaaggagt acatcttcta cgacgggcca
      901 ccgttcccca ccgggagccc gcactacggc aacctcctcg ccggggtgat caaggacatg
      961 gttccccggt actggacgat gcgcggctat cgcgtcgaca ggcgatttgg ctgggacacc
     1021 cacggacttc ccatcgagat ggaggtagaa aagcgcctcg ggatctccgg tccgaaagcg
     1081 atcgccgaat atggcgtcga ccgattcaac gaagctgccc gcagtgtggt tcaggccaac
     1141 accgagaact gggaggtcct gaccgagcgc atcggccgtt gggtcgactt cgagaacgac
     1201 tacaagactt tggaccccga gttcatggag acggtctggt gggtcttcaa acagctctgg
     1261 gacaaggggc tcgtctacaa ggatttcaag gtcctgccct actcgtgggg ggcggccacg
     1321 ccgctctcca acttcgaggc gaacaaggat tatcgagatg tagaagatcc ctcgataacg
     1381 gtcagactcg aagtgctcga aagtgccggt ccgatgacgg ccggcgacta catcctcatc
     1441 tggacgacga ctccttggac gcttcccggg aacctggcag tggctgtcgg ggacgaaatc
     1501 gcctactccc gaatcaccga cggcgatgac cactactgga tcgccacggc gcgagccgcg
     1561 accgtgtttg gcgaagatcc gaacgtggtc gccgaggcca agggatccga gatcctgggg
     1621 gtcgcctatc tacctccatt cgattacttc agcgccgagc gagaggcggg ggcgttccgc
     1681 atcatctcct ccccagaggt gagcgtcgac gaggggacag ggctggtgca catggcgccg
     1741 gcctacggag aggccgattt ctacgctctc aagggagccg gcatcgactc attggtcgac
     1801 ccggtcgacg ccgaagggga cttcaccgat caggtgcccg aagtcgccgg tcagaacgtg
     1861 aaggtggccg acgagacact catcgagctc ctgcgggcct cggggagcct ggttcattcc
     1921 gaccggatca cccactcata ccccttctgt gagcggaccg acaccccact gatctacaag
     1981 gcgatcccca cctggttcat ccgggtcgag gacatcaagg accgaatggt ggagatcaac
     2041 gacaccatcc actgggttcc cgactacgtg ggaagtcgcc gcttcggcaa ctggctggtg
     2101 aatgcgcggg actgggcggt cagtcgcaat cgctactggg gctcatgcat cccggtctgg
     2161 gaatgcactg ggtgcgacga agctgtcgcc gtcggctccc tcgacgagtt ggaggctctc
     2221 tccggccatc gacccgccga tctccacaaa cacgtcgtcg acgacatcgg ttggcagtgc
     2281 tcctcctgcg gtggttcgat gatcagggtc cccgaggtcc tcgatggctg gttcgagtcg
     2341 ggctcaatgc cctacgggat gctccattac ccgttcgaga acgccgagcg attcgaggcc
     2401 gcattcccgg ccaaattcat cgccgaggga ctggaccaaa cccggggctg gttctacacc
     2461 ctcatcgtcc tctccaccgc cttgttcgac gctccggcgt tcgagaactg cgtggtcaac
     2521 ggtctgatcc ttgccgaaga cggccggaag atgtcaaaga gcctgaagaa ctaccccgac
     2581 ccttcggaga tcttcgacca catgggggcg gacgcattgc gcgcttttct catcaactcg
     2641 ccggtgctcc gggccgagcc actccgcttc agcagtgacg gggtggcaga agtcgtgcgc
     2701 acagttctcc tcccgatgtg gaacacatac agcttcttca ccacctacgc cgaggcggac
     2761 ggcatgacag tcgccgacct cgggatggca cctcctcccg ccgaccggcc ggagatggat
     2821 cgatggatcc tgtccgttct gcagagcctg gtgtccgagg tcaacaccca gatggagggc
     2881 tattacctct acaacgtcgt gcccccgacc ctgggtttca tcgatcacct caccaactgg
     2941 tacgtgcgtc ggtcccgccg ccgtttctgg cgggcccgtg gcgagtccgg tgagggcgac
     3001 accgacaagc tggcggcctt tgccaccctc tatgaagtgc tcaccaggtt catcgaggtg
     3061 ctcgctccgg tgctgccgtt catgacggag cgcctctacc aagatctagt cgcccgtcat
     3121 catcccgacg ccgttcccag tgtccatcac cgcgatttcc ccgaagccgc gatccccctc
     3181 atcgaccggg atctggaggc tgcgatggag gtggtccggg acgtggttcg cctcgggcgg
     3241 aacctgcgca agccagaggg gctccgcgtg cgccagccgt tgtcgaagct gacggtgctc
     3301 acccgtagct cgtccgtggc cctggcggtc gagacccatt cggatgtgat cgccgatgag
     3361 ttgaacgtca aggaggtggc gacttcgtcc gacgacgggt cgttggttca cctgtcggcc
     3421 aaggccaact tccgcacgct gggccccgtt ttcgggccca ggatgcccga ggcagccagc
     3481 gccatcgctg atctggggcc ggcggatctc gaagtcgtcc tcgacggcgg cactgtctcc
     3541 atcggcggac atgacctcgg catgggggac ctgataatcg accggacccc ccgagaggag
     3601 accgtcgtag aggcgggctc cgatctcgcg gtggctctcg acactcacgt atccgatgag
     3661 ttgctcctcg agggcatcgc cagggaactg atcagccggg ttcagaggct gcggcgggag
     3721 gcgagcctgg ac
//
LOCUS       DASTXO010000321         3386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_240300, whole genome shotgun sequence.
ACCESSION   DASTXO010000321 DASTXO010000000
VERSION     DASTXO010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240300"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VFU96_06860"
     CDS             complement(<1..578)
                     /locus_tag="VFU96_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113024.1"
                     /translation="MAQVLDPPAPVEVELRPAPPLDMRRAVKLGLAGGAAMVLVAATG
                     IVATFQPRMVIDPVVPLGIMFLYLLAFGFGWMAGQPPATLEGYEPATAGSRNVLAGAL
                     AGLLAGLSLGLLLIVITVFDLRGVFINLSPELADILGFGLPVAAAVPLIALVSSAVGA
                     LGAAVHLLNRRVRRTLMGALTAVIVFSLLTDL"
     gene            complement(582..1670)
                     /locus_tag="VFU96_06865"
     CDS             complement(582..1670)
                     /locus_tag="VFU96_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU5113025.1"
                     /translation="MTEAATVEARPARRASRITFVGVFLWAARIAILAIILYGSYLSI
                     SSGRLSAAQWRDLFVFGISTGSMYALIALGYTMVYGVLRFINFAHGEVFMVGAMMAFY
                     VANALAITPLWTSSPAVSLLIVLVAAMFTSTAIAVIIERIAYRPLRGAPRLIPLITSI
                     GASFTIQYLVRGLVGPATKSFPRMEPLRGTVEFGGFVILKTQLVVIVAALVMMGGLYL
                     FVEKTRTGKSMRAVAEDPETAALMGINVDRTIVNVFAIGGAMAGVAGALWGLVFPSIN
                     FFSGFLPGIKAFTSAVLGGIGNIVGAMLGGVSLGIFESVGPSLVLAGYEIPAFSQLKD
                     VVAYAALVLVLIFRPTGILGERLAQERT"
     gene            complement(1750..3096)
                     /locus_tag="VFU96_06870"
     CDS             complement(1750..3096)
                     /locus_tag="VFU96_06870"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU5113026.1"
                     /translation="MKMKRGLKLLALVGVLSLVIAACGGGEGGDTSTTAAEAETTTSA
                     AAEETTTSAAGETTTAPAEGLGTIEVAAGDPIKIASLQSISGETASLGTDQVRAIEIA
                     IADKGEILGHPVELAFNEDDGCNSEGGQTGSTRIIADPQVVAVIGTSCSGAGVPASNV
                     ITSAGRVLISGSNTSPVLTSDLAGNEASEHHVGYYRTAHNDAIQGAAAAKFVFEELGL
                     TKVATINDGDPYTQGLTSAFEGPFTELGGEIVLSTAVTADQTDMRSVLTEVAAAGAEL
                     IFFPIFQPAGDFIAEQALEVAGLEETVLMGADGLLSDTYVVLPQTENMYFSGPQTPTT
                     DAYAEFVTKYTEAYGEAPIQSFHAHSYDATNMVFNAIETASTDDGGTLTIDLQAFRDA
                     LNEMTYEGLTGTLSCDDFGDCASPNIDIVQNTAETADIEGVRGNVLISYSREDLGL"
BASE COUNT          646 a         1044 c         1120 g          576 t
ORIGIN      
        1 aacaggtcgg tgagaaggga gaacacgatc acggcggtga gcgctcccat caaggtccgg
       61 cggacacgtc ggttgaggag atggactgcg gcccccaggg cgcccacggc cgacgacacc
      121 agtgcgatca ggggaactgc cgcagcgacc ggtaatccga agccgaggat gtcggccagc
      181 tccggagaca ggttgatgaa cacgcctcgt aggtcgaaga cggtgatgac gatcaagagc
      241 aagccgagtg acaaacctgc caggagcccg gcgagtgcgc cggcgaggac gtttcgagac
      301 cctgctgtgg ccggctcgta gccttccaga gtcgccggtg gctggcccgc catccaaccg
      361 aagccaaagg cgagcagata gaggaacatg atccccagcg gaacgaccgg gtcgatcacc
      421 atccggggct ggaaggtggc cacgatgccg gtggcggcga cgaggaccat cgctgcacct
      481 cccgccaatc ccagtttcac tgcccttcgc atatcgagag gaggcgccgg ccgtagctcg
      541 acttcgaccg gtgcaggtgg gtccaagacc tgagccatgg ttcaggtcct ttcctgggcg
      601 agtcgctcgc cgaggatgcc ggtgggtcgg aagatgagca ccagaaccag ggcggcgtag
      661 gccacgacgt ctttgagctg actgaacgca gggatctcgt agccggcgag gacgaggctt
      721 gggccgaccg actcgaagat cccaagactg acgccaccga gcatggcgcc gacgatgttg
      781 ccgatgccgc cgaggacggc tgaggtgaaa gctttgatgc cgggaaggaa ccctgagaag
      841 aagttgatgc tggggaacac cagaccccag agtgcgccgg ccacaccggc catcgctccc
      901 ccgatcgcga agacattgac gatcgtgcgg tcgacgttga tgcccatcaa tgcggcggtc
      961 tcaggatcct cggcaacggc tcgcatcgac ttgccggtcc ttgtcttctc cacgaagagg
     1021 tagaggccgc ccatcatcac aagagcagcg acgatcacca ccagctgggt cttgagaatg
     1081 acgaacccac cgaactcgac ggtgcctcgc aacggctcca tgcgtgggaa cgacttggtg
     1141 gccgggccta cgagcccgcg aacgaggtac tggatcgtga acgatgcgcc aatggaagtg
     1201 atcaaaggga tgaggcgcgg ggccccacgt agaggccgat atgcgattcg ctcgatgatg
     1261 accgcaatcg cggtcgaggt gaacatggct gccaccagga cgatcaggag agagaccgcg
     1321 gggctggacg tccagagcgg ggttatggcc agggcgttgg ccacgtagaa ggccatcatc
     1381 gcccccacca tgaagacctc gccatgggcg aagttgatga agcgaagcac gccatagacc
     1441 atcgtgtagc ccagggcgat aagggcgtac atcgagcccg tggagatccc gaagacgaag
     1501 aggtctcgcc actgtgcggc actgagtctt cccgacgaga tgctcagata cgacccgtag
     1561 aggattatcg cgaggatcgc gatccgagca gcccagagaa agacacctac aaaggtgatc
     1621 cgtgaggcgc gccgtgccgg gcgggcttcg acagtggcgg cttctgtcat ctagcgggcg
     1681 gcctccccgt ttgttagagg cccggcgccg tgttggtcca cggcgccggg caatagggcg
     1741 agccccaggt cagagaccca ggtcctccct cgagtagctg atgagaacgt tgccgcgaac
     1801 cccttcgatg tcagcggttt cggcggtgtt ctgaactatg tcgatgttcg gggaggcgca
     1861 gtcgccaaaa tcatcgcagc tcagcgtgcc ggtcagaccc tcgtaggtca tttcattcaa
     1921 ggcatcccgg aacgcctgca ggtcgatggt cagcgtccca ccgtcgtccg tcgatgcggt
     1981 ctcgatggcg ttgaacacca tgttggtcgc gtcgtacgaa tgagcgtgga acgactggat
     2041 cggagcctca ccgtacgcct cggtgtactt ggtgacgaac tcggcatagg catcggtggt
     2101 cggagtctgc ggaccagaga agtacatgtt ctccgtctgc ggcaacacca cgtaggtgtc
     2161 ggacagcaga ccatcggcgc ccatcaggac cgtctcttcc agcccggcca cttccagggc
     2221 ctgctcggcg atgaagtcgc cggcgggctg gaagatcggg aagaagatca gctccgcacc
     2281 tgcggctgcg acctcggtca gcaccgaacg catgtcggtc tggtcggccg tcacagccgt
     2341 gctgaggacg atctccccac cgagctccgt gaaggggccc tcgaaggcgc tggtgaggcc
     2401 ctgggtgtag ggatcaccat cgttgatcgt cgcaaccttg gtcagcccga gctcctcgaa
     2461 cacgaacttg gcggcggcgg caccctggat ggcgtcgttg tgagccgtcc ggtagtagcc
     2521 gacatgatgc tccgaggcct cgttaccagc caggtcggaa gtcagcaccg gcgaggtgtt
     2581 ggaccctgag atcaggaccc ttcctgccga ggtgatgacg ttcgatgccg ggacgcctgc
     2641 gcccgagcag ctggtgccga tcacggcaac gacctgcgga tcggcgatga tccgggtgga
     2701 acccgtctgt ccgccttccg agttacagcc gtcgtcctcg ttgaaggcga gctcgacggg
     2761 atggcccagg atctcaccct tgtcggcgat ggcgatctcg atcgcccgca cctggtcggt
     2821 gccgaggctg gccgtctcac cgctgatcga ctgcaacgac gcgatcttga tggggtcgcc
     2881 cgcggcgacc tcgatcgtgc ccaggccttc agccggagcc gtcgtcgtct cgccggccgc
     2941 cgaggttgtg gtctcctccg cggcggcgct ggtggtcgtc tcggcctcag cggccgtcgt
     3001 gctggtgtct ccgccctctc cgccgccaca agcggcgatg acgagggaga gaacacccac
     3061 gagcgcgaga agctttaggc ctctcttcat tttcatatgt ctttcctcca tgtggaatgc
     3121 gcggcgagac agggagccac gccgcgctgc gactttatac tcaaattcgc actcagtcac
     3181 ccgggaacgg ccctctcatc acccctgcgc gcgtaccgtt gcgcggtggt gtcggtgccc
     3241 aaagtcgtcg aggatgcccg cgaatctccc gggccgtggg gcccaaaacc cgctctgggc
     3301 tggctcgtcg taggtctgat tctgggtgcc ggtctgaccg tgttggtgct gcggtcaggc
     3361 ccgccggagg aatcggtcac gagcac
//
LOCUS       DASTXO010000322         2403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_19632, whole genome shotgun sequence.
ACCESSION   DASTXO010000322 DASTXO010000000
VERSION     DASTXO010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19632"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..1285
                     /locus_tag="VFU96_06875"
     CDS             257..1285
                     /locus_tag="VFU96_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113027.1"
                     /translation="MGNDQRHADTVRVKKQARPWTGFFLGLLLGLAIAVLLQQAGVWP
                     LDRLLLYGSAGLFALIGILMGGAGRERAGAFASIVPLVLAVALIGWGATGIADINESG
                     EVNGGCTVQAESSVDSTVVTDTSRQDPFQIDPEGSLAWVAASPAPITNHFWEIYVDVG
                     GFPIVVASNDEAELNTDEDTENTGEVADISAYVAEVSNYAGLELDGVFEVGGSIEGDG
                     GTCDGFGFVELTAGPLTTLVSQIAAAVGLLALIGLLVLVFNRTREASVVPETVVDDDM
                     APDFDETDAAPPVDGPTAATAAGGVAGSAGGGAHVRPEDVETERPPQPSEEPEPDDRA
                     EPDSDGER"
     gene            1337..1621
                     /locus_tag="VFU96_06880"
     CDS             1337..1621
                     /locus_tag="VFU96_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004012668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEU5113028.1"
                     /translation="MSTGPVQLTEELAAAWRERAACSGYRHTLFFPVGDTDAATIDRA
                     LEICMGCAVIDDCLEYALETNQRAGIWGGTTEDERRSLRRKWLATRRRVG"
     gene            1672..>2403
                     /locus_tag="VFU96_06885"
     CDS             1672..>2403
                     /locus_tag="VFU96_06885"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5113029.1"
                     /translation="MALLFFVNGVVVGSWLPRLPEIRDRLGIDLGALGLTLALGGLGS
                     LVGSSLSGLVVGRFGARRTAVIAGAAVYFVLPLIAIAPVAAVLAIVLAVIGFIDAQAD
                     VGMNAVGVRVEEAVGRSVMTRLHGLWSLGTLIGSGLSALAVLVGLGLGLQLLIVSVIG
                     VGSAVYAGRLIPDATPRRREGQRSGRLAVGLMLAGAFAIVIEGAPFDWSALYLADFTG
                     SSDALAGVGVIVFTAGMLIGRLAGDH"
BASE COUNT          387 a          764 c          824 g          428 t
ORIGIN      
        1 tcatacagcg tggtgcgtcc gacgccggac gcgagggcga tctcgcccaa cgggatctcg
       61 gcggtgcccg cgtcggcgat cagcgacctt gccgatgcca gcagagaggc acgggtcagt
      121 gccttgtgct cgtcgatcga cgtcgcgcgg atccggggca tgtgcagaga gtatcggccc
      181 cgccagggaa cggcagccag ttgtcgccga tcggatcgag gcaggacgta gattctcacg
      241 cagacaggag gacccgatgg gcaacgacca acgacacgct gacacggttc gcgtcaagaa
      301 gcaggcccga ccctggaccg gtttcttctt ggggctcctc ctcgggctcg cgatcgcggt
      361 gctccttcaa caagcgggcg tctggcccct ggacaggctt ctcctctacg ggtcggccgg
      421 cctcttcgcg ttgatcggga tcctgatggg cggggcggga agagaacggg cgggagcatt
      481 cgcctccatc gtgcctctcg tgctggccgt cgccctcatc ggatggggcg caactggcat
      541 cgccgacatc aacgagagcg gcgaggtcaa cggcggatgc accgtgcaag ccgaatcatc
      601 agtggacagc accgttgtca ccgacaccag ccgccaggat ccgttccaga tcgacccgga
      661 aggaagcctt gcctgggtgg ctgcgtcacc ggctccgatc accaatcatt tctgggagat
      721 ctacgtggac gtaggcggct tcccgatagt ggtggcctcc aatgacgagg ccgagctcaa
      781 caccgacgag gacaccgaga acacaggtga agtcgccgac atatccgcct acgtggccga
      841 ggtctcgaac tacgccggtc tggagctgga tggcgtgttc gaagtcggag gcagcatcga
      901 gggcgacggt gggacgtgcg acggtttcgg attcgtcgag ctgaccgcgg gcccgttgac
      961 gacgctcgtc tctcagatcg ccgcggcagt cggtctgctg gccttgatcg gccttctggt
     1021 cctcgtcttc aaccgcaccc gggaagcctc cgtagtcccc gaaactgtcg tcgatgacga
     1081 catggcaccc gacttcgacg aaacggatgc cgcccccccg gtcgatgggc cgactgccgc
     1141 cacggccgcc ggcggggttg ccgggtccgc gggtggagga gcccatgtcc gaccggaaga
     1201 cgtcgagacc gagcgacccc cgcagccgtc tgaggaacca gagcctgacg atcgggccga
     1261 gcccgactcc gacggtgaga gatgacgaga aactcgtcgt cttggcaaac aaggggatca
     1321 acgcgaaact cgaggcatga gcacgggccc agtgcagctg accgaggagc tggcggcagc
     1381 gtggagagag cgagccgcct gttcgggtta ccgccacaca ctcttcttcc cggtcggcga
     1441 cactgacgcc gccaccatcg atcgcgccct cgagatctgc atgggctgcg ccgtcatcga
     1501 cgactgcctc gaatacgccc tggagacgaa tcagagggca gggatctggg gtggcaccac
     1561 cgaagatgaa cgacgttcgc tgagacggaa atggctggcc acgcggcgtc gcgtcggctg
     1621 actcgctgag aggatcccga tctgaacggc accaggaggg ccattctcgg ggtggcgctc
     1681 ttgttcttcg tgaacggggt cgtggtcggc agctggcttc cccgtctgcc cgagatccgg
     1741 gaccggctcg gaatcgacct cggcgcgctc ggcctgactc tcgctctcgg tgggctcggg
     1801 tccttggtcg ggtcttcatt gagcggtctc gttgtcgggc gttttggcgc tcgccgcact
     1861 gctgtgatcg ccggtgccgc tgtctacttc gtcctcccgc tcatcgcgat cgccccggtg
     1921 gcggcagtgc tggccatcgt cctggccgtc atcggcttca tcgacgccca agccgatgta
     1981 ggcatgaacg ccgtcggcgt ccgcgtggag gaggcggtgg gtcggtcggt tatgacgaga
     2041 ctccacgggc tgtggagtct gggcaccctg atcgggtccg ggctgagcgc tctggccgtc
     2101 ctcgtcggcc tcggtctcgg cctccagtta ctgatcgtct ccgtaatagg ggtcggatcc
     2161 gctgtgtacg ccggacgtct gatcccggat gcgaccccgc gtcggcgcga gggccagcgg
     2221 tcgggccgcc ttgccgttgg actgatgctg gcaggcgcgt tcgccatcgt catcgaggga
     2281 gcgccgttcg attggagcgc cctgtatctg gccgacttca ccggcagtag tgacgccctc
     2341 gccggcgtgg gtgtcatcgt cttcacggcg gggatgctga tcgggcgact ggcaggggat
     2401 cat
//
LOCUS       DASTXO010000323         4003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1309035, whole genome shotgun sequence.
ACCESSION   DASTXO010000323 DASTXO010000000
VERSION     DASTXO010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309035"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VFU96_06890"
     CDS             complement(<1..836)
                     /locus_tag="VFU96_06890"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEU5113030.1"
                     /translation="MEVSNPDRVVFPEDAITKGDVVDYYIGVADRMLPFIEGRLLSVE
                     RFPRGIAGQGFMQKNVPDHAPEDLIGRHEVPKEGGGTTVYPMVDSAEGIAFFANLGVI
                     TFHVPPVRADDEEHPDWVIWDLDPPADRVELVREAAREMHAILETFGIETVLMTSGSK
                     GYHLRALLEPTTNGEDVAFVARGTAALGAAANEDLLTLAFRKADRGDRVFVDWMRNTP
                     RSTSVVPWSLRPKTGAPIATPIGWPELGDVEPAGVRLTNVRDRSRKRPWTKLESIDLS
                     PAV"
     gene            complement(914..1942)
                     /locus_tag="VFU96_06895"
     CDS             complement(914..1942)
                     /locus_tag="VFU96_06895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5113031.1"
                     /translation="MFSPIRTERLLIRPFHEDDLAAYFDRRNDPDVARYQDWELPYPV
                     KRAEKVVSAVVAMDGPEDEHWWMAVVCDPVTGEVFGDVGAELSWQGRTAEVGYTFAKE
                     HWGQGYATEALEALVAYLFDEIDVTRVCGMLHPDNPASAMVLERTGLLFEGETKSSFW
                     VGDELSDDWIYGMTRPDWETWRDRPRHTPEEVHLVEVTTENEAIVSGLTTHRTQEEFV
                     APMLRSFADALFPEVVDGAPVVPWMRAVVADRAIVGFVMLALTTEHHPEPFLWRLLID
                     RLHQRRGIGARALDLVGAECVAMGDKTLLTSWGEGKGSPRPFYLAQGFVPTGRSIDEE
                     TEGRKRLS"
     gene            1975..2199
                     /locus_tag="VFU96_06900"
     CDS             1975..2199
                     /locus_tag="VFU96_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010063153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HEU5113032.1"
                     /translation="MSDDKIERVQGPASYFPSIEKKYGRPIAEWQALIRSSDLTKHME
                     LVNWLKSEYGMGHGHANALVAYTLAQDAGA"
     gene            2268..2621
                     /locus_tag="VFU96_06905"
     CDS             2268..2621
                     /locus_tag="VFU96_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009632747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113033.1"
                     /translation="MRIQTGQLVALGYGAYVRSDDVTAVAPITEGRGPGRRSLVWVRG
                     MTDPLVASRAEGSIVDDMVTPADEAARMKAQRSVLNKMATVLDKVSPGIRRLLAEESG
                     TDIDDLLAEASRVLG"
     gene            complement(2623..3063)
                     /locus_tag="VFU96_06910"
     CDS             complement(2623..3063)
                     /locus_tag="VFU96_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017947349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEU5113034.1"
                     /translation="MTIEERAVDPSRPHAAEHVRQYLASDGASVDHPAAGHLILLYTT
                     GRVSGSIRRTPLRFFEIEGEAVVAASFGGSPRHPDWYLNLLDDPRVWVRRDAELYEAD
                     ATPIEGGERDLLWNTVVVPMAPQFAAYQEKTERIIPLVRLVARS"
     gene            3101..>4003
                     /locus_tag="VFU96_06915"
     CDS             3101..>4003
                     /locus_tag="VFU96_06915"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113035.1"
                     /translation="MRRLAAFLLIASAACTQAAVENPSTSSTPAAGSATGAPTGWVEL
                     APMAEPRSEHPAAVLGGEVLVAGGLIEVGVGTTGVTPTVEAYSPETDTWRALEDLPEA
                     RHHGMAASVGERLFFIGGYTPSGDPSESVWELVEDEWVDRNILPGPVAAGAAVTVGDA
                     IFVVGGVPEGNVYRYDVSDDTWSDAPPPGTRREHVAAVALDGDIWAIAGRWEGEIFDT
                     TEIYDPDAQAWRAGPSLSDARSGFGAVVVDQAIVVAGGEVFSPDEALDSVERLEDAGE
                     QWASIESLPHGRHGNPLVAIGHEIY"
BASE COUNT          725 a         1316 c         1280 g          682 t
ORIGIN      
        1 acggccgggc tcaagtcgat tgattcgagc ttcgtccacg gtcgtttccg cgaccggtcc
       61 cggacattcg tgagcctgac tccggccggc tcgacgtcgc ccagttccgg ccagccgatc
      121 ggtgttgcga tgggtgcacc cgtcttgggc cggagggacc aggggaccac cgacgtcgac
      181 cgcggggtgt tccgcatcca gtcgacaaag acccgatcgc cgcggtcggc cttgcgaaac
      241 gccaaggtca acagatcttc gttggcggct gctccgaggg cagctgtgcc tcgtgcgacg
      301 aaggcgacgt cttcgccgtt ggtggtcggc tccagcaggg cacgaaggtg ataacccttc
      361 gagccggatg tcatgaggac ggtctcgatg ccgaacgtct cgaggattgc gtgcatctcg
      421 cgggccgcct cccggaccag ctcgacccgg tcggccggcg ggtcgaggtc ccagatcacc
      481 caatccggat gctcctcgtc gtcggctcga acaggcggca cgtgaaaggt gatcaccccc
      541 agattggcga agaacgcgat cccctccgca gagtcgacca tcgggtagac agtcgtcccg
      601 cctccctcct tgggcacctc atgacgcccg atcaggtcct ccggggcatg gtcgggaacg
      661 ttcttctgca tgaatccctg acctgcgatc ccgcgcggga accgctcgac cgagaggagg
      721 cggccctcga tgaaggggag catgcgatcg gccacgccga tgtaatagtc gacaacgtcg
      781 cctttcgtga tcgcgtcctc ggggaagacc acccggtctg gattggagac ctccacggac
      841 ccaaacctag gtcgacccga cctgcagcgt cacaaagccg gatcgaggag ctgcggctcg
      901 tggcgaggcc ttctcacgac agccgtttcc gcccttcggt ttcctcgtcg atcgacctcc
      961 cggtcggaac gaagccctga gcgagataaa agggccgcgg agagcctttg ccctcacccc
     1021 aactcgtcaa gagggtcttg tcacccatgg cgacgcactc ggcgccgacc aggtccaggg
     1081 cacgcgctcc gatcccgcgt cgctggtgga gacggtcgat caggagacgc cacaggaacg
     1141 gctccggatg gtgctcggtg gtcagggcca gcatcacgaa accaacgatg gcccggtcgg
     1201 cgacgacagc tcgcatccac ggcacgactg gtgctccgtc caccacctca gggaacagcg
     1261 cgtcggcgaa cgagcgaagc atcggggcga cgaactcttc ctgcgtcctg tgggtggtga
     1321 gaccggacac gatcgcttcg ttctcggtgg tcacctcgac gaggtggacc tcttcgggag
     1381 tgtggcgggg ccggtctcgc cacgtctccc agtccggtcg cgtcatcccg tagatccaat
     1441 cgtccgacaa ctcatcgccg acccagaacg aggatttcgt ctcgccttcg aagagcaatc
     1501 cggtgcgctc caggaccatc gccgaggccg gattgtccgg atggagcatt ccgcacaccc
     1561 tggtcacatc gatctcgtcg aataggtagg ccaccagggc ttccaaagcc tcggtcgcat
     1621 atccctggcc ccaatgttcc tttgcgaacg tatagcccac ttcagcagtg cgaccctgcc
     1681 aggagagctc tgctcccacg tcaccgaaga cctcgcccgt gacggggtca cacacgacgg
     1741 ccatccacca atgttcgtcc tcagggccgt ccatcgccac caccgcggac acaaccttct
     1801 cggctcgctt cacgggatag ggaagctccc agtcctgata gcgagccacg tcgggatcgt
     1861 tacgtcggtc gaagtaagcc gcgaggtcgt cctcgtggaa aggccggatg agcagccgct
     1921 cggttcggat cggggagaac accccgccga ttcaaccagc gatagccttc gcctatgtct
     1981 gatgacaaga ttgagcgcgt ccaaggaccc gcgtcctact tcccttcgat cgagaagaag
     2041 tacggccggc ccatcgcaga atggcaagcc ttgatccgct ccagtgacct caccaagcac
     2101 atggagctcg tcaactggct caagtccgaa tatggcatgg gccatgggca cgccaacgcc
     2161 ctcgtcgcct acacgcttgc gcaggatgcc ggcgcgtgag ccccgcacag ggggcgcctg
     2221 atcctgtccg gcgatgccgg tagcttccga catcgacagg aggaaagatg cgcatacaga
     2281 ccggtcaatt ggtcgccttg gggtacgggg cctatgtgcg ctccgacgac gtgaccgcgg
     2341 tggcgccgat caccgaggga cgagggccgg gaagaagatc cctggtttgg gtccggggaa
     2401 tgaccgaccc tctcgtcgcc tcacgggccg aggggtccat cgtcgacgac atggtgactc
     2461 cggccgatga ggcggcgcgg atgaaggcac aacgctctgt gctcaacaag atggcgaccg
     2521 tcctcgacaa ggtctcaccg ggaatcaggc ggctactggc ggaggaatcg ggcaccgaca
     2581 tcgacgacct gttggccgag gcaagccggg tcctcggctg actcagctac gagcgaccag
     2641 ccgcaccaga gggatgattc gctctgtctt ctcctgatag gccgcgaatt ggggcgccat
     2701 cggcaccacc accgtgttcc acagcaagtc ccgctcccct ccctcgatcg gagtggcgtc
     2761 agcctcatag agctccgcgt cccgtctcac ccagaccctg gggtcatcga gcaggttgag
     2821 gtaccaatcc gggtgccggg gtgaccctcc gaacgaggcc gccacgaccg cttcaccttc
     2881 gatctcgaag aaccgcaacg gtgtgcgtcg gatcgagcct gaaacccgac cggtggtgta
     2941 gagcaggatc agatggcctg ccgccgggtg gtccacagac gctccatcgc tcgcgaggta
     3001 ctgacggaca tgttcggcgg cgtggggacg cgaagggtcg acggcgcgtt cttcgatcgt
     3061 catcgctcga acctatcacc gccgttcgta ggctggtgga gtgcgccgtc tcgccgcatt
     3121 cctcctcatc gcttcagcag cttgcaccca agccgcggtc gagaatccct ccacttcctc
     3181 cacaccggcc gccggatcgg ccacgggagc tcccaccggc tgggtagagc tggctccgat
     3241 ggccgaaccc cgaagcgagc acccggctgc ggtcctcggg ggagaagtct tggtcgcagg
     3301 agggctcatc gaggtcgggg tcgggacaac cggcgtgacg cccacagtgg aggcctactc
     3361 gcccgagacc gacacctggc gtgccctcga ggatctcccc gaggcacgtc atcacgggat
     3421 ggcggcatcg gttggagagc gtctcttctt catcggtggg tacacgccgt ccggagatcc
     3481 ctcggagtcc gtatgggagc tggtggaaga tgagtgggtc gaccgcaaca tcttgcctgg
     3541 gccggtggct gcgggcgctg cagtgacggt gggcgacgcg attttcgtcg tcggtggggt
     3601 ccccgaaggg aatgtgtacc gctacgacgt gtcagacgac acgtggagcg acgcaccccc
     3661 cccgggcaca cggcgggaac atgtcgccgc cgtcgcactc gatggcgata tctgggccat
     3721 cgccggacgc tgggaaggcg agatcttcga caccactgag atctacgacc cggatgcgca
     3781 ggcctggcgg gccggccctt ctctcagtga tgcacgttcc gggttcgggg cggtggtggt
     3841 cgatcaagcg atagtcgtgg ccggaggtga ggtgttctcg cccgacgagg ccctggactc
     3901 ggtggaaaga ctggaagatg ccggcgaaca atgggcgtcg atcgaatcgc tcccccacgg
     3961 ccggcacgga aacccactgg ttgccatcgg ccacgagatc tac
//
LOCUS       DASTXO010000324         2193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1750747, whole genome shotgun sequence.
ACCESSION   DASTXO010000324 DASTXO010000000
VERSION     DASTXO010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1750747"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..711
                     /gene="pyrR"
                     /locus_tag="VFU96_06920"
     CDS             178..711
                     /gene="pyrR"
                     /locus_tag="VFU96_06920"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014134540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEU5113036.1"
                     /translation="MPQVMNGQDIQRAVRRIAHEILERHRGAETVVIVGIHTRGVHLA
                     RLLGKLIAEFEGVQVPVGELDIGNYRDDHALRPVHEAAVTEIPADISDRSVIVVDDVL
                     YTGRTVRAALDALADIGRARQTELAVLVDRGHRELPIRADYVGKNLPTSQEEVVRVRI
                     LPVDQAEGVWVERNGAA"
     gene            708..1601
                     /locus_tag="VFU96_06925"
     CDS             708..1601
                     /locus_tag="VFU96_06925"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU5113037.1"
                     /translation="MSGLISLRGLTRERLEDLLDRAQSFVGSPVTDRHAGATVTTAFF
                     EPSTRTRLSFERAASRLGVHVMDLSPDLSSTTKGESFKDTILTLTAIGTDLFVIRHWL
                     ADACEMAAQWSGRPVINAGVGKREHPTQTLIDALTLRQEFGHLDGLRMAIVGDISNSR
                     VARGHLDALPVLGVELTLVGPTPFLPIANPWGVKVSIDLDDELGDTDVVYLLRIQSER
                     GGSDGIPANEAYARRYGMSDERLSMLKPAAVIMHPGPINRGVEIEDAAADGERSLILA
                     QVANGVPVRMAVISEALGGLG"
     gene            1598..>2193
                     /locus_tag="VFU96_06930"
     CDS             1598..>2193
                     /locus_tag="VFU96_06930"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5113038.1"
                     /translation="MIVVRNARLLRPDGIEQATVTIEDDTIVSVGTDPPSNGARVIDA
                     KGSWLGPGLVDLHVHLRDPGQTWKEDVASGADAAAAGGFTAVVAMPNTHPPVDGGSSV
                     IGASERAKTVDSVDIVVAGTLTKGRAGVEMADFDAMYEAGVRVFSDDGDSVPDAGLLR
                     RVMAYLRDLPGALVAEHAEDRSIAAGGHMHEGMVSSRLG"
BASE COUNT          384 a          683 c          743 g          383 t
ORIGIN      
        1 cggcacgaag ctgacgttcg ccttcgatca cagcctgccg gcgatgtagt tgctccacat
       61 agaagtttgg gatcgcaatc gttgccgggc taaggtcgcc acgcctttaa aacgcggtcc
      121 ggtgaggccg gaaaggagtg acatgggccc tgtcccatcg gcgtcggagt cgagccgatg
      181 ccccaggtga tgaacggcca ggacatccag cgagccgtgc gccgcatcgc ccatgagatc
      241 ctcgagcgtc atcgcggcgc cgagaccgta gtcatcgtcg gcatccacac ccgtggtgtc
      301 caccttgccc gtctgctcgg aaagctgatc gccgagttcg aaggcgtcca ggtcccggtc
      361 ggcgagctcg acataggcaa ttaccgcgat gaccacgccc tgcgtccggt tcacgaagcc
      421 gccgtgaccg agatccccgc ggacatctcc gatcgcagtg tgatcgtcgt cgatgacgtg
      481 ctctacaccg gcaggaccgt gcgggctgcc ctcgatgcgc tcgccgacat cggccgggcg
      541 cggcagaccg agctggctgt cctcgtcgac cgcggtcacc gggagctccc gatcagggcg
      601 gattacgtcg gcaagaacct gccgaccagc caggaggagg tcgtccgggt tcggatactc
      661 ccggtcgacc aggccgaggg ggtttgggtg gagaggaacg gagcggcatg agcgggttga
      721 tcagcttgag gggcttgacc cgggagcgcc tggaggactt gctcgacaga gctcagtcgt
      781 tcgtgggaag cccggtgacg gatcggcatg ccggcgccac ggtgaccacc gcattcttcg
      841 agccatcgac ccgaaccagg ctgtcgtttg agcgggctgc atcccggctc ggtgtccacg
      901 tcatggatct cagccctgat ctgtcttcga ccacgaaagg cgaatcgttc aaggacacga
      961 ttctgaccct cacggccatc ggaaccgact tgttcgtgat ccgccactgg ttggccgacg
     1021 cttgcgagat ggcggcccaa tggtcggggc gtcccgtgat caacgccggg gtagggaaga
     1081 gggagcaccc aacgcagact ctgatcgacg ccctcaccct ccgccaggaa ttcgggcatc
     1141 tcgacgggct tcgaatggcc atcgtcggcg acatcagcaa cagcagggtg gctcgcggcc
     1201 atctcgacgc gctcccagtg ttgggggtgg agctgacgct ggtcgggccg accccgtttc
     1261 tccccatcgc caacccctgg ggggtgaaag tcagcatcga tctcgacgac gagctcggtg
     1321 acaccgatgt ggtctatctg ctccggatcc agagcgagcg tggggggtcg gatgggattc
     1381 cggccaatga ggcatacgcc cggcgctacg ggatgagcga tgagcgcttg tcgatgctga
     1441 aacccgctgc cgtcatcatg cacccgggtc cgatcaatcg cggcgtcgag atcgaagacg
     1501 ctgcagccga tggggagcga tcgctcatcc tcgcccaggt ggccaacggt gtgccggtgc
     1561 gcatggccgt catctccgag gctctcggag gactcggatg atcgtcgtcc gaaatgccag
     1621 gctgctgcgc cccgatggga tcgagcaagc caccgtgact atcgaggatg acaccatcgt
     1681 ctctgtcggc acagatccgc cctcgaacgg agccagggtg atcgatgcca aagggtcctg
     1741 gctcggtccg ggcctggtgg atctccacgt tcatctgcgc gacccgggac agacctggaa
     1801 ggaagacgtg gcctctggcg ccgatgcagc agcggctggg ggtttcacag cggtcgtggc
     1861 catgcccaac acccacccgc cagtcgatgg ggggtcgtcg gtgatcggcg catccgagcg
     1921 ggccaagacc gtcgactccg ttgacatcgt cgttgcgggg actctgacca agggccgcgc
     1981 aggtgtggag atggcggatt tcgacgccat gtacgaagcg ggggtgagag tgttcagcga
     2041 tgacggcgat tcggtgcccg acgccggatt gctgaggcgg gtcatggcct atctgcgcga
     2101 tctgcccggc gccctcgtcg cggagcacgc cgaagatcgc tcgatagcgg ctggcggtca
     2161 tatgcacgaa gggatggtct cctcgcgtct ggg
//
LOCUS       DASTXO010000325         2361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_240384, whole genome shotgun sequence.
ACCESSION   DASTXO010000325 DASTXO010000000
VERSION     DASTXO010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240384"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1722)
                     /locus_tag="VFU96_06935"
     CDS             complement(118..1722)
                     /locus_tag="VFU96_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113039.1"
                     /translation="MVLRLVELKARLTWNGFRHDRQRRIGLPIATILLSWLGLWLAQS
                     HYQNIILLRESNPTALAEYLGWTALIAFLVWVTLPVIIFPLDENLDPQQLATLPLAPN
                     QMITGLAASSLVAPSTVVPIALLAANALALGGAWWMAIPASLVFLALLAVGGQLFSAA
                     VSGVLRTRRGRDIATFLILGLAAGSFFGYRMVSAAVAEQGLAGAVTETPITGWWALLP
                     PVAAQKAIVDASSGRAGAGLLALAAASVGLILLAITWRRLLGWLLSTPVQQSRPAARA
                     RRGGMTIGPWGVVPTMARKELRFYVRDPRQRLVWTGTVIFVGLAVGGAVMNATGFFDL
                     RDRAWAPLIAPILVLFVGLPIALNLFGWERNAASYLFVLPPKPHQILLGKNLAVAGAL
                     VVETTVLTLGLAIFTGQWTWVWLVVPLTVAAIGCQLAVGNVVSVLTPLRLPREGTDVF
                     AQSTEQGCLAIVSQTVSFFAIGFLLVPPASVVVLTVDFGQVLAPWVAVMSSIAWGLIL
                     YGISLFVSSRLLRRRMPEVLTWVQVT"
     gene            complement(1704..>2361)
                     /locus_tag="VFU96_06940"
     CDS             complement(1704..>2361)
                     /locus_tag="VFU96_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5113040.1"
                     /translation="LLGQNGAGKTTTIKTIVGLLRPDEGSVGIGEFDTWKSPTEVKAR
                     IGVLPEEFNLYERLTGAELLDFTAAMHGLDRMEAAKRRNELLDLLDLVDAGSKLVGDY
                     SRGMRKKIALGAAIIHRPPVLFLDEPFEGVDAVSARLIRRLLQQAAHGGTTIVFSSHE
                     MHLVEKLCTRIGIMVAGRLTIEETPEGLCRRMGVDNVEDAFIAAVGGRTPDSSWYSGS
                     "
BASE COUNT          478 a          733 c          794 g          356 t
ORIGIN      
        1 agcaactcgt gctcgagccg ctcccgatcg acgcgggtgc gtcgaagcat caaatcctcc
       61 gatgccacat gccggtgaaa ctagacgcga gctggaaccg aggacgaaag cgctgcgtca
      121 ggtcacctga acccacgtga gcacttcggg catccggcgc cggagcagac gactggagac
      181 gaacaatgag atcccgtaca ggatcagacc ccaggcgatg gaagacatca ccgccaccca
      241 gggggccagg acctggccga aatcgacagt cagcacgacg accgaggccg gggggaccag
      301 caagaagcca atggcgaaga aggagaccgt ctggctgacg attgccagac aaccttgctc
      361 tgtcgattgg gcgaagacgt cggtcccttc tcgggggagg cgaagcggcg tgaggacaga
      421 gaccacgttg ccaacggcga gctgacagcc gatggcggcc accgtcaggg ggacgacgag
      481 ccagacccat gtccactggc cggtgaagat ggcgagaccg agtgtgagca cggtggtctc
      541 gacgacgagg gcgcctgcca ccgccagatt cttcccgaga aggatctgat gtggcttcgg
      601 cggcaggacg aacaaatagc tggcggcgtt gcgttcccat ccgaacaggt tgagggcgat
      661 cggtagaccg acgaagagga cgaggatcgg ggcgatcaac ggggcccagg cccgatctct
      721 caggtcgaag aacccggtcg cgttcatgac cgcaccgcca acggcgagac cgacgaaaat
      781 taccgtcccg gtccacacca gccgctgtct tggatccctc acgtagaaac gcagctcttt
      841 gcgagccatc gtgggcacga ccccccacgg accgatcgtc atgccaccac gacgagcacg
      901 tgccgccggt ctgctttgct gaacgggcgt cgacaggagc catccgagca ggcggcgcca
      961 ggtgatggcc aggaggatga gaccgactga cgcggcagcc agggcgagga ggcccgcacc
     1021 cgcccgccca ctggaggcgt cgacgattgc cttctgggct gccaccggtg ggagaagagc
     1081 ccaccagccc gttatcgggg tctctgtcac cgccccggcc aggccctgtt cggccactgc
     1141 cgcgctcacc atgcgatatc cgaagaagga gcccgcggcg agaccgagga tgaggaacgt
     1201 ggcgatgtcg cgccctcgtc tggtgcggag cacgccagag accgcagccg aaaacagttg
     1261 gccgcccacc gcgagcagcg cgaggaagac gagtgacgct gggatcgcca tccaccaagc
     1321 acctcccagg gcgagggcat ttgcggcgag tagagcgatc ggcactacgg tcgaaggtgc
     1381 gacgaggctg gaagcggcga ggccggtgat catctgattc ggcgcgagcg gaagcgtggc
     1441 cagctgctgt gggtcgagat tctcgtcgag tgggaagatg atgaccggga gcgtcaccca
     1501 gaccagaaac gcgatgagcg cggtccagcc gaggtactcg gcgagagctg tcggattgga
     1561 ttccctgagc aggatgatgt tctgatagtg ggattgggct agccagagcc cgagccaact
     1621 caggaggatc gtcgcgatcg gcaggccgat ccgcctctga cgatcgtggc ggaacccgtt
     1681 ccaggtgagg cgagccttga gctctacgag ccggagtacc acgacgagtc cggagtccgc
     1741 ccgcccacgg cggcgatgaa ggcatcttcg acgttgtcca cacccatccg tcggcacagg
     1801 ccttcggggg tctcttcgat cgtgagcctg cccgccacca tgatcccgat cctggtgcac
     1861 aacttctcca ccaggtgcat ctcgtgcgag gagaacacga tggtcgtgcc cccgtgcgcc
     1921 gcctgttgga gaagacgacg gatcagccgc gcggagaccg catccacgcc ttcgaaaggc
     1981 tcgtctagaa acagcaccgg tggacgatgg atgatggccg cacccagggc gatcttcttc
     2041 cgcattccgc gtgagtagtc gccgacgagc ttcgagcccg cgtcgacgag atcgaggaga
     2101 tcgaggagct cgttgcggcg cttggccgcc tccatgcggt cgagaccgtg catggctgcg
     2161 gtgaagtcga ggagctcggc gccggtcaga cgctcgtaga ggttgaactc ctcaggcagg
     2221 acaccgatcc gagccttgac ttccgtcggg ctcttccagg tgtcgaattc gcctatccca
     2281 acgctgccct cgtccgggcg gaggagcccg acaatggtct tgatggttgt cgtcttcccg
     2341 gcgccgtttt ggccgaggag g
//
LOCUS       DASTXO010000326         2432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_278140, whole genome shotgun sequence.
ACCESSION   DASTXO010000326 DASTXO010000000
VERSION     DASTXO010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278140"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VFU96_06945"
     CDS             complement(<1..684)
                     /locus_tag="VFU96_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase cyclase
                     subunit"
                     /protein_id="HEU5113041.1"
                     /translation="MASRVIPCLDIKDGRVVKGVRFQGLRDAGDPVECAELYEAQGAD
                     EIVLLDVSATPEGRAHALGTVREVRAMVSIPLTVGGGVRRAEDAGRLLEAGADKVGVN
                     TAAVERPALIEKIAGQYGNQCTVLAVDAASTGQGWEVVIESGRSRTGVDVVEWCRKAT
                     TMGAGEILLTSWDRDGTREGYDLALIAAVSDAVTVPVIASGGANEPGHLIDALRAGAS
                     AALVASILHD"
     gene            complement(687..1295)
                     /gene="hisH"
                     /locus_tag="VFU96_06950"
     CDS             complement(687..1295)
                     /gene="hisH"
                     /locus_tag="VFU96_06950"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEU5113042.1"
                     /translation="MTEVRIVPTGTANIASVRAAMTRLGARVSDATTPREVTDAAGVI
                     LPGVGAFGAAMSRVEDRGLGRALRQRIRQGLPTLMVCVGMQLMCRESEESPGAKGLDI
                     LDTTVIRFGDGVRVPQLGWNQIEPGPGSRFVEPGWAYFANSYRINQVPDGWVGATTEY
                     GGGFVSALERGHVLACQFHPELSGAWGARLLGRWLTTAEEAA"
     gene            complement(1292..1876)
                     /gene="hisB"
                     /locus_tag="VFU96_06955"
     CDS             complement(1292..1876)
                     /gene="hisB"
                     /locus_tag="VFU96_06955"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012289034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEU5113043.1"
                     /translation="MTRRSEINRETTEVTITGYLDLDGTGTSEVKTGLGFLDHMLATL
                     ARHGGFDLGLVATGDTDVDDHHTVEDCAIVLGRALDGALGDRTGIARFGYGYAPLDES
                     LSRAVVDLSGRPWPEVSIDFSRPTIGEVATENIIHFLRSFAIEGRMALHVDLIRGEND
                     HHKAESAFKALAIALRAAVSQSGAGIPSTKGTLS"
     gene            complement(1873..>2432)
                     /locus_tag="VFU96_06960"
     CDS             complement(1873..>2432)
                     /locus_tag="VFU96_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEU5113044.1"
                     /translation="TPSDIEAAKADGNSNDDWELTRRLCLERGVDAPIEAVTDRFETL
                     YQCTGASPGLKMAERSLVEVPTWQRWAARWPLAVVTGRPRSDAVEFLERFALMEDITA
                     LITREDAPLKPDPAPVLLALDSMGVRQAWMLGDTPDDLAAARAAGVVPIGVIAPGDDP
                     AGARERLGRAARILDKTTDLEEILP"
BASE COUNT          388 a          852 c          757 g          435 t
ORIGIN      
        1 atcgtggagg atgcttgcca cgagagccgc cgaggcgccg gctcggagcg cgtcgatcaa
       61 gtgtccgggc tcgtttgccc caccggatgc gattactggc accgtcacgg catcggagac
      121 tgctgcgatc agggcgaggt catagccctc gcgcgtcccg tcccggtccc agctggtgag
      181 caggatctca ccggcgccca tggtcgttgc ctttcggcac cattccacga catcgacacc
      241 ggtgcggctc cgtccgctct cgatgaccac ctcccatccc tgcccggtcg atgcggcgtc
      301 aaccgccagc accgtgcact gattgccgta ttgcccggcg atcttctcga tgagggcggg
      361 acgttccact gcggccgtgt tcaccccaac cttgtcggca ccggcctcga gaagtcgtcc
      421 cgcgtcttcg gctcgacgca cgccaccacc tacggtcagc gggatggaaa ccattgcgcg
      481 cacctctcga acggtgccca aggcgtgggc tcttccctcc ggtgtggccg agacgtcgag
      541 gaggactatc tcatccgctc cctgcgcctc atagagttcg gcgcactcca cgggatcgcc
      601 cgcgtcgcga agaccctgga aacggacccc cttgaccacc ctcccgtcct tgatgtcgag
      661 acacgggatc acacgggaag ccagggtcac gccgcctcct ctgccgtcgt cagccatcgg
      721 cccaagagac gtgctcccca tgcccccgac aactcggggt ggaactggca ggcgagcacg
      781 tgaccccgtt ccagggccga gacgaatcca ccgccgtact cggtcgtggc accgacccag
      841 ccgtcgggca cttggttgat tcggtagctg ttggcgaaat aggcccagcc cggctccacg
      901 aacctcgacc ccgggcccgg ctcgatctga ttccatccca attggggcac cctgaccccg
      961 tctccgaaac gaatcaccgt cgtgtcgagg atgtcgaggc ctttggcacc ggggctctct
     1021 tcgctctccc ggcacatcaa ttgcatgccg acacaaacca tgagcgtcgg gagtccttgt
     1081 ctgatccgct gacgcagcgc tcggcccaag cctcggtctt ctacccgcga catcgcggcc
     1141 ccgaaagcgc cgaccccggg gaggatcacc ccggcggcgt cggtgacctc tcgaggcgtc
     1201 gtcgcgtcgg agaccctggc accgagacgg gtcatcgcgg cccgcaccga ggcgatgttg
     1261 gccgtgccgg tggggacgat cctcacttcg gtcacgacag ggtccccttg gtgctcggga
     1321 ttccggctcc cgactggctc accgccgcac ggagggcgat tgccagagcc ttgaacgccg
     1381 actcagcctt gtggtggtcg ttctctccac ggatcaagtc gacgtggagc gccattcgac
     1441 cctcgatggc gaatgatctg aggaagtgga tgatgttctc ggtggcaacc tcaccgatgg
     1501 ttggtcggga gaagtcgatc gacacctcag gccaggggcg tccggagagg tcgaccaccg
     1561 ccctgctcag cgactcgtcg agcggggcat agccgtagcc gaatcgggca atgcccgtgc
     1621 gatctcccaa agccccgtcc agggcccggc ccaggactat ggcgcaatcc tccaccgtgt
     1681 ggtggtcgtc cacgtcggtg tcgccggttg ccaccaggcc gaggtcgaat ccgccgtgtc
     1741 gcgccagggt cgccagcatg tggtcgagaa agccaagtcc ggtcttcacc tcggatgtcc
     1801 ctgtcccgtc caggtccaga tatccggtga tggtgacctc ggtggtctcc cgattgatct
     1861 cggatcgtcg tgtcatggca ggatctcctc caggtcagtg gtcttgtcga gaatgcgggc
     1921 ggccctcccg agccgctccc tggcacctgc tgggtcgtca ccgggtgcga tgaccccgat
     1981 cggcacgaca cccgcggccc tcgccgcagc caggtcgtcc ggagtgtcac caagcatcca
     2041 tgcctgcctc acccccatgc tgtcgagagc gagcagcacc ggtgccgggt cgggcttgag
     2101 cggggcgtcc tccctggtga tgagtgccgt gatgtcttcc atcagggcga agcgttcgag
     2161 gaactcaaca gcatcggatc gcggccgccc ggtcaccaca gcgagaggcc acctggccgc
     2221 ccaccgttgc caggtcggga cctcgaccag ggaccgttcc gccatcttga gcccgggcga
     2281 tgccccggtg cattgataca gggtctcgaa ccgatctgtc actgcctcga tgggggcgtc
     2341 cactccgcgc tcgaggcaga gccggcgcgt gagctcccaa tcgtcgttgc tgttgccatc
     2401 ggccttggca gcctcgatgt cggaaggtgt ga
//
LOCUS       DASTXO010000327         2691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1088169, whole genome shotgun sequence.
ACCESSION   DASTXO010000327 DASTXO010000000
VERSION     DASTXO010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088169"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..1166
                     /locus_tag="VFU96_06965"
     CDS             198..1166
                     /locus_tag="VFU96_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113045.1"
                     /translation="MSDLEVIEAGVDAFYSGNAERAAELFELADRTDDQIRAESAYQA
                     AIGGQVALSCTESAPGSFNCSTPYRNAMTEAIGEGGGHDVWPVVVEDGVITQFGFTEH
                     TGMLIEMGTFLASEGRFDGYEDCVSGPFPESCATIQMENLDAWAAWHDTSEPADRVET
                     VVESWYGGNCEAVAVMSWGNEDCSASSLPAQTMAYESILGAQVSVENCATTSGGDNPV
                     LSCEVHYSNAMNSAVGKPPSVTAREFVLRNGRSAFLVGPDDEPWYQADYPEDTELRDS
                     FRLFAEGGELADEYADASCASARSPECANLIVDNLDAWATWYETNG"
     gene            1418..2050
                     /locus_tag="VFU96_06970"
     CDS             1418..2050
                     /locus_tag="VFU96_06970"
                     /inference="COORDINATES: protein motif:HMM:NF014301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA domain-containing protein"
                     /protein_id="HEU5113046.1"
                     /translation="MALSLLSGLMVVTAGSAVAKPSGGNNSADAKLCKNWQSLYKEDG
                     SGFADKGDCTSHAAEGGTILTSPPPPPALPYQVTILPPSPGSGNYEAAGATFGPTPTQ
                     AGVTGPLFLANDGNGTSQGCSTASFAGMPAGSIAIVDAGGCEFTFKALNAQANGAVAV
                     IVLNNVPGDPVTMEGTAPSLGIPAVMVSQEDGFVIKATLIGGSGIVSAAP"
     gene            complement(2194..>2691)
                     /locus_tag="VFU96_06975"
     CDS             complement(2194..>2691)
                     /locus_tag="VFU96_06975"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU5113047.1"
                     /translation="KLHEPVEFTEDLGLFASFLVWAIFGALLLAPQLRGGVSPTAIIY
                     AVLSLTLVRIVPVAVAMIGTDLRPASLLFMGWFGPRGLASVVFTLIAFESLREGGLAP
                     DALIEVATWTVFLSVIAHGITAGPLAIRYGRRVSDEPVEQLEAGAHLGDPVPRALTDR
                     PASQS"
BASE COUNT          511 a          807 c          907 g          466 t
ORIGIN      
        1 gatccattcc cgctgatgtg attgagcggc tagtcgagaa agagggagac atgatgagaa
       61 ggtcgagatc ggcaggagtc gcagcggctc tgctcgtgtt gacggcgtgc gcgagcgggg
      121 gagccgcaac aacgacggaa cccaccacaa ccgccgcacc gaccacaacc gccgcacctg
      181 ccaccagcgc gcccaccatg tccgatctgg aggtgatcga ggccggtgtg gatgcgttct
      241 actccgggaa tgcggaacgg gccgccgagt tgttcgagct cgcggaccgc accgacgatc
      301 agatacgcgc cgagtcggcc tatcaggcgg cgataggtgg gcaagtggcc ctgagctgca
      361 cggagagcgc gcccgggtca ttcaactgca gcacgccgta ccgcaacgcg atgacggagg
      421 cgatcggcga aggcggcggc catgacgtgt ggccggtcgt ggtcgaggac ggggtgatca
      481 cacagttcgg attcaccgag cacacgggga tgctgatcga gatggggacc ttccttgcga
      541 gcgaaggtcg cttcgacggt tacgaagact gcgtctctgg tccgttcccc gagtcgtgcg
      601 ccaccatcca aatggagaac ctcgacgcct gggcggcgtg gcacgatacg tcggagccgg
      661 cagacagggt ggagaccgtc gtggagtcct ggtacggagg gaactgtgag gcagtcgccg
      721 tgatgtcgtg gggcaacgaa gattgctcgg catcgtcgtt gccggctcaa acgatggcgt
      781 acgagtcgat cctgggtgca caggtctccg tcgaaaactg tgcaaccacc tctggcgggg
      841 ataacccggt tctctcatgt gaggtgcatt actcgaatgc catgaacagc gcggtgggta
      901 agccgccctc ggtgaccgcc cgagagttcg tcctcaggaa cggccgcagt gcattcttgg
      961 tagggccgga tgatgagccc tggtatcaag ctgactaccc ggaagacacc gagctccggg
     1021 acagcttccg gctgttcgcc gaagggggcg aactggctga cgagtatgcg gacgcaagct
     1081 gcgcatccgc acgctcaccc gagtgcgcca acctcatcgt ggacaacctc gacgcctggg
     1141 cgacctggta cgagaccaac ggctgaccaa ccagtcgacc gatcgctcct gagcgagcgg
     1201 tgatggcgcc ctgggcagga ttcgaacctg cgagaccccg ctcatgcacg acgacccagc
     1261 cggactcgtc ccgcagcatt gcggtgcggc ggccgttacc ttgccttcgg catctgcttt
     1321 cgcaggtgca gggacctggg acattcccgg gcgggtgaat ggaggaatct tggtagggag
     1381 taaccgtacg cggtcacgcc gcgtcgttcc gttgttggtg gctttgagcc tgttgtccgg
     1441 gttgatggtg gtgacggcag ggtcggcagt tgcgaagccg tcggggggca ataacagcgc
     1501 cgatgccaaa ctgtgcaaga actggcagtc cctctacaag gaggacgggt cgggattcgc
     1561 cgacaaaggc gactgcacca gccacgcggc tgagggtggc accattctga cctcgccgcc
     1621 tcccccacca gcactgccct atcaagtgac aatccttccg ccttcgcctg gctcgggaaa
     1681 ctatgaggcc gcgggcgcga cctttggtcc gaccccgacc caggccgggg tgactggccc
     1741 actgtttctc gccaacgatg gcaacgggac gtctcaggga tgttccacgg cttccttcgc
     1801 cggcatgcct gctggctcga tcgcaattgt cgatgctggc ggatgtgaat tcactttcaa
     1861 ggctttgaac gcccaggcga acggagcggt cgctgtcata gtcctcaaca atgttccggg
     1921 cgacccggtc accatggagg gcaccgcgcc gtctttggga atcccggcgg tgatggtgtc
     1981 acaagaagac ggattcgtca tcaaggccac gctcatcggc ggctcgggca ttgtgtctgc
     2041 cgccccgtaa cgggcgaatc gtctcgcctg gtgctgctca ggcaaaatcc gcgatgggat
     2101 ttcgggcgcc tcggcagggt tcgacctgcg acacccggtt caagtctcgg gccttcccga
     2161 tggatgacgg cgtgcgttgg gtcctgttca tggtcaagat tgcgatgccg gcctatcggt
     2221 gagggcgcgg gggaccgggt cgccgagatg tgctccggct tcgagttgct cgaccggctc
     2281 gtcggagact cgcctcccat atctgatggc cagggggccg gcggtgatgc cgtgggcgat
     2341 gaccgagaga aaaaccgtcc acgtggcgac ctcgatgaga gcgtctgggg cgaggccacc
     2401 ctcacggagt gattcgaagg cgatcaaggt gaacacgacg gaagccaatc cgcggggacc
     2461 gaaccacccc atgaacagga ggctggccgg ccgtaagtcg gtcccgatca tcgccacggc
     2521 caccggcact atccggacca gggtgagact caggaccgcg tagataatcg ccgtgggcga
     2581 caccccgcct ctcagttggg gggcgaggag taaggccccg aagatggccc agaccaggaa
     2641 cgaggcgaat aaaccgaggt cctcggtgaa ttcgacaggc tcgtgcaatt t
//
LOCUS       DASTXO010000328         3084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1346468, whole genome shotgun sequence.
ACCESSION   DASTXO010000328 DASTXO010000000
VERSION     DASTXO010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346468"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VFU96_06980"
     CDS             complement(<1..356)
                     /locus_tag="VFU96_06980"
                     /inference="COORDINATES: protein motif:HMM:NF013383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEU5113048.1"
                     /translation="MSRRIAVLGAGAIGSSVGADLAEAGEDVVLIDQWPAHVDAMRNG
                     GLRVVMPGKDLRVKVRALHLGELAAERPVFDVVLLAAKSYDTVWMCHLIARYLANNGV
                     FVGLQNGMNEETITPIV"
     gene            complement(353..1285)
                     /locus_tag="VFU96_06985"
     CDS             complement(353..1285)
                     /locus_tag="VFU96_06985"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU5113049.1"
                     /translation="MKDLSVDALMPTGIFERLDPHFAELSSTHLSTLLRRPELDSRTR
                     FLILTGQYTVTGKHHPLEVTIEAALDAGVRPSDLLEVILQCYVYTGPWVVAAAGEVYL
                     GVLERRPELADDLAARVDDGAGRSLEKERATWNEADRDDPRLAPLLEKYGWDGISTGL
                     RLRPRHHINLVATLDALDPEYLQNWLDAIYGGMYVRRVLDDRTRILCVVGNTLAAGEQ
                     HQSRRHMRSALRSGATPKELLEVIFQTTGLFGHTHLMPAAIDDLVRIADEEGRLDQLL
                     APDRIDEVRRIVAARVASRGGVAEIDLNTLEGKE"
     gene            complement(1282..2292)
                     /locus_tag="VFU96_06990"
     CDS             complement(1282..2292)
                     /locus_tag="VFU96_06990"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5113050.1"
                     /translation="MIDSSHGKVVDVHAHLLPRSAIAAAESGAEWFGSRIETDSLGRP
                     EIVTGDYRVSMGGPAHWESIEQRITKMDAIGVDRQVISLNPILFRYYLDSSDAIECSK
                     AVNDEIAGIVADRPDRFAGFGTLPMQDVGAAMKELERVMSTKGMVGVSVGTHVGGLNW
                     DEPDLFPILEAAEQSGGMVFIHPAASRLKEALPRYHLRNFIANPTETTIAIGSLIFGG
                     ILDRLPALRLLLAHGGGYACWAYARFDHGHEVRAEARNHIARLPSDYLSSMWYDSLVH
                     GYGNLRRLVDVVGIDRVVLGTDFPADMGQPDPVSWIEGSDLSTAEKDAVLGSNAEVML
                     PW"
     gene            complement(2289..>3084)
                     /locus_tag="VFU96_06995"
     CDS             complement(2289..>3084)
                     /locus_tag="VFU96_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEU5113051.1"
                     /translation="ERNVMDALEQVSFAASQGAEVVAFPETYPGPWRTPMTFDPTEAM
                     AAAAKEHGVTVQFGTLEAVAEEPEAAYNVLKLVGPDGTISGVYRRTHPPGPWIYAGGV
                     WDFDYRVGDEYPVFGSEHGTVGLAMCSEAFMPEVARALALRGAEIIFMPAGIDKKKLW
                     AAWRNLIWARATENLAVVITTQNLFDESERGLAMVAQPEEIVFEADGPGTFLIDVDLT
                     RIREMRGQEDGPKSSEEYGAKPGVLTQWQRPEMHDKVYPAVTRTPT"
BASE COUNT          524 a         1030 c          978 g          552 t
ORIGIN      
        1 acgatcgggg tgatcgtctc ctcgttcatc ccgttctgca gcccgacgaa cacaccgttg
       61 ttggcgaggt agcgggcgat gagatggcac atccagaccg tgtcatagga cttggcggcg
      121 aggagcacca cgtcgaagac cggtcgctcg gcggccagtt cgccgaggtg gagcgcccgc
      181 accttcaccc tcagatcctt gcccggcatg acgacccgca gcccgccatt ccgcatcgcg
      241 tcgacgtggg ccggccattg gtcgatcagg acgacgtcct caccagcctc ggccaggtcg
      301 gcgccgacac tgctcccgat agccccggcg ccgagcaccg cgatcctcct gctcattcct
      361 tcccctccag ggtgttcaga tcgatctcgg cgacaccacc ccgactggcc acccgtgctg
      421 ccacgatccg ccgaacctca tcaatgcggt ccggagccaa cagctggtcg agacgaccct
      481 cttcgtcggc aatccgcacc aagtcgtcga tcgccgccgg catgagatgg gtgtgcccga
      541 acagaccagt ggtctggaag atcacctcga gcagctcttt cggtgtcgcc ccgctgcgga
      601 gggccgaccg catgtgacgg cgcgactggt gctgctcccc cgctgccagc gtgttgccta
      661 ccacgcagag gatcctggtg cgatcgtcga gcactctcct gacgtacatc cctccgtaga
      721 tggcgtcgag ccagttctgc aggtattcgg gatcgagggc atcgagggtg gccaccagat
      781 tgatgtggtg ccggggtcgg agccggagcc cggtcgagat cccgtcccag ccgtacttct
      841 cgaggagcgg cgcgagccgg ggatcgtcgc ggtcggcctc gttccaggtg gcacgttcct
      901 tttcgaggct gcggccggcg ccatcgtcga cacgagccgc caggtcatcg gctagttccg
      961 gtcttcgctc caggactccg aggtagacct cgccggcggc ggccaccacc cagggaccgg
     1021 tgtagacgta gcactggaga atgacttcca gaaggtcaga ggggcggacc ccggcatcca
     1081 gggcagcctc gatcgtcacc tcgagtgggt ggtgcttccc ggtgacggtg tactggccgg
     1141 tgaggatcag gaacctggtc cgggagtcga gctccggtcg gcgaaggagc gtcgacagat
     1201 gggtcgagct cagctcggcg aaatgaggat cgagtcgctc gaagatgccg gtcggcatca
     1261 acgcatcgac tgacagatcc ttcaccatgg gagcatcacc tctgcgttcg agccgagcac
     1321 cgcgtccttc tccgcggtgg acaggtcact tccctcgatc caggacaccg ggtcgggctg
     1381 acccatgtct gccggaaagt cggtccccag caccacccgg tcgatcccga cgacgtcgac
     1441 gagccggcgc aagttgccgt acccgtggac cagcgagtcg taccacatcg agctcagata
     1501 gtcggaggga agccgggcga tgtggtttcg agcctcggcc cgaacctcgt gcccgtgatc
     1561 gaaccgggcg tacgcccaac atgcgtagcc gccgccatgg gccaagagga gacgaagggc
     1621 gggaaggcga tcgagaattc cgccgaagat gagggacccg atggcgatcg tggtctcggt
     1681 gggattggcg atgaaattgc gcaagtgata tcgaggtagc gcctctttca gacgggatgc
     1741 ggccgggtgg atgaacacca tccctccgga ttgctcggcg gcttccagga tcgggaagag
     1801 gtcgggctca tcccagttca gcccaccaac gtgggtgcca acgctgaccc cgaccatccc
     1861 cttggtggac atcacccgct ccagttcctt cattgccgca ccgacgtcct gcatcgggag
     1921 agtcccaaac ccggcgaacc gatctggacg gtcggcgacg atgccggcga tctcgtcgtt
     1981 gaccgccttg gagcactcga tggcgtcgct cgagtcgagg tagtagcgga acaggatcgg
     2041 attgagcgag atcacctgcc ggtcgacacc gatggcgtcc atcttggtga tcctctgctc
     2101 gatcgactcc caatgggccg gccctcccat cgacacccgg tagtcgccgg tcacgatctc
     2161 gggtcggccc aaggaatctg tctcgatgcg ggacccgaac cattcggccc cgctttccgc
     2221 cgcagcaatc gccgatcgtg ggagaaggtg agcgtggacg tcgaccacct tcccatggga
     2281 gctgtcgatc acgtcggggt gcgggtcacc gcgggataga ccttgtcgtg catctcgggt
     2341 cgttgccatt gggtgagcac accaggcttg gccccatact cctccgacga cttggggcca
     2401 tcctcctgcc cccgcatctc ccggatcctg gtgaggtcta catcgatgag gaaggtgccg
     2461 ggcccgtcgg cctcgaacac gatctcttcg ggttgcgcca ccatggccaa tccgcgctcg
     2521 gattcgtcga agaggttctg ggtcgtgatc acgacggcga ggttctccgt ggcccgtgcc
     2581 cagatcaggt tccgccaggc cgcccacaac ttcttcttgt cgattccggc gggcatgaag
     2641 atgatctccg cccctcggag cgccagggct cgggccactt cgggcatgaa cgcttcgctg
     2701 cacatcgcca gcccgacggt cccgtgctcg ctaccgaaca ccggatattc gtcgccgacc
     2761 cggtagtcga agtcccaaac gccaccggcg tagatccagg gccccggcgg gtgagtacgc
     2821 cggtagacgc cgctgatggt cccgtcgggc ccgaccagct tgaggacgtt gtaagccgcc
     2881 tcgggctctt ccgcgactgc ttccagcgtt ccgaactgga ccgtgacacc gtgctccttc
     2941 gccgcggcgg ccatcgcttc cgtggggtcg aatgtcatcg gcgtccgcca cggtcctgga
     3001 tacgtctctg ggaacgcaac tacttcggca ccctgggagg cggcgaacga gacctgctcc
     3061 agtgcgtcca tcacgttccg ctcg
//
LOCUS       DASTXO010000329         4960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_315167, whole genome shotgun sequence.
ACCESSION   DASTXO010000329 DASTXO010000000
VERSION     DASTXO010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315167"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VFU96_07000"
     CDS             complement(<1..294)
                     /locus_tag="VFU96_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113052.1"
                     /translation="MIEGLTEYAREHWPFWFGHFPESVGIAIGSGGPAHRTRAAVFVF
                     ENRRTRPSAVMKIAFTELEAGFLRHEHDALTNVRPLLPERMRPTLPRVLGLAEI"
     gene            complement(291..803)
                     /locus_tag="VFU96_07005"
     CDS             complement(291..803)
                     /locus_tag="VFU96_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113053.1"
                     /translation="MSEAPLDESLNPIWVLLSRGERPNIWMSPPGGTDWAGSLHSALT
                     ALGDDELIVSIGRPLARSALARLSGRGASSGKGPSPAEVRDAFGDESLEVVETYGLWP
                     SARSPRIAFPWGHIHMLYWLQTSGVLGGGGSRLWARILARSVLFTPVAAALAPGIAFV
                     VRRSGRGDTS"
     gene            complement(800..2050)
                     /locus_tag="VFU96_07010"
     CDS             complement(800..2050)
                     /locus_tag="VFU96_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5113054.1"
                     /translation="MSTGGVLFLNPNRARGQTAPVASWISVASWAAAARRLWGKSWIV
                     TPEGVLEPEEIMRRASDPVTLKGPPGPPSLRRKVPTTVKTGIKDARKALEARQFREAG
                     LDGPWRSEDVRFVWQRHDLFHDAGVTAARALKRPLVVFTPALIVQEAAGWGVKRPGWQ
                     GILERSAEAPLLRAADVVACGSVEIAEQVGRLGVPGERLLVTPNGVDPEMFSPERHGH
                     EVRRRLGLEERFVIGWVGSFRKFHGLDMALEAMKRIETEIPEATLLLVGDGLERAHVE
                     DSVRDLGLRNVVMTGTVGYLEIPEHVAAMDVAVITDRGTGSFHYSPLKLREYMASGKA
                     VIAPRIGEMARWLVDGEDAILIESGDTEGLASAIQELHGRPDLRSRLGTAARAKILDQ
                     ATWDIQLLRIDEALQAAPRSRSPE"
     gene            2148..3938
                     /locus_tag="VFU96_07015"
     CDS             2148..3938
                     /locus_tag="VFU96_07015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5113055.1"
                     /translation="MPETAEGRAFRVAHLTTVDLSLRFLVRPQLLAVNEAGGEAVGVS
                     APGPWVEELERDGIRHVALTASTRGMSVGNDLKAIIQLWRVLRTEEFAVLHTHNPKPG
                     VYGRILGRLAGVPLVVNTLHGLYATEDDTPVKRGVVYALEAVAARFSDVELHQNEEDL
                     AFCRRTRILPAGKGQLLGNGVDLVRFDRDKVDPGARTRLRAEFGAGDSDVVVGMVGRL
                     VAEKGYPELFEAATKLGEGYVVVVIGPDDPEKSDALPPDAVANAQAAGVRFLGMRTDV
                     EDLYSAMDLFVLPSHREGFPRAAMEAAAMGLPIIATDIRGCRQVVDDGVNGLLVPVLD
                     PDRLGEAIRKVGDDPTRREKMSEASARIARERFDEQRVVDIVMDAYRDGLVAKGLGHL
                     MPPAMIAPAPRRQMRLATSRDARALAELHTSQIATGFLPLLGRGFMNVLYQALIAWKG
                     AVVVVVDDGGGPVGFTAGVENVGEFYSHFARHYGWRAGIAALPRLIRPSNLRRAWETF
                     RYGQNQVDVPAELLSMVVAPRARGKGLSLMLGARLLDDLSRRGVPAVKVTVGSENETA
                     ISVYRKMGFKDTERIQVHAGESSEVLVWRL"
     gene            3926..4888
                     /locus_tag="VFU96_07020"
     CDS             3926..4888
                     /locus_tag="VFU96_07020"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEU5113056.1"
                     /translation="MAALIVAGVVSLAWCFFAIWLGPVFGYVDRPDDSALTAHERPAV
                     PLGGVGVFLGVNAAGFMRSDLELGLLIATTMVLILGLIDDRRGIDPKMRLGVELVAAV
                     VLVVNVAGDPGIVYVVLGIALAVFAINSVNLFDGLDGLVGSVGLVTAAGLAVIAQGRG
                     LDFVSPLELGAALAGFLVLNWHPARVFMGDAGAYFLGLYLTHLIIDSTNDSALELVLT
                     AGVLGVFAIDMLVTLLRRRLNHRPLFLGDRSHIYDQLRDRGLGVRSIVVIAATTQAIL
                     LAFVIAAERLLGVEWGLVTLSGLLALLLAGLARLGFLRVDQART"
BASE COUNT          811 a         1601 c         1631 g          917 t
ORIGIN      
        1 gatctcggcc aatcccaaca cccggggcag ggtcgggcgc atgcgctccg gcagcagagg
       61 gcgaacattg gtgagggcat cgtgctcatg cctgagaaac ccggcctcga gctcggtgaa
      121 cgcgatcttc atcaccgccg agggccgagt tcgtctgttc tcgaacacga acacggccgc
      181 cctcgtccgg tgagccggcc ccccgctccc gatcgcgata cccaccgact cgggaaagtg
      241 accgaaccag aacggccaat gttccctggc gtactccgtc agcccttcga tcacgaggtg
      301 tccccacgac ctgaacggcg gacaacgaag gcgatgcctg gggccagggc ggcggccacc
      361 ggagtgaaaa ggaccgacct ggccaggatt cgagcccaca gcctgctccc cccgcccccg
      421 agcaccccgg aggtctggag ccagtagagc atgtgaatgt ggccccaggg gaaggcgatt
      481 cgcggagacc gggccgacgg ccagaggcca tatgtctcga ccacttccag gctctcatct
      541 ccgaaggcat ctcgcacctc ggccgggctg gggcccttgc cggaggaggc tccccggccg
      601 gaaagccggg ccagcgcgga gcgggccaag ggacggccaa tcgacacgat cagctcgtca
      661 tcgccgaggg cggtcaatgc cgagtgcagc gaacccgccc agtccgtccc tcccggaggc
      721 gacatccaga tatttgggcg ttctcctcgg gagaggagaa cccaaatcgg attcagagac
      781 tcgtctagcg gtgcctcgct cattccggag accgggatct cggcgccgct tgaagcgcct
      841 cgtcgatgcg aagcagctga atgtcccagg tcgcctgatc gaggatcttg gcgcgagcgg
      901 cggtaccgag gcggctcctg agatcgggcc gcccgtggag ctcctgaatc gctgaggcca
      961 ggccctcggt gtcgccgctc tcgatgagga tggcgtcttc cccgtccacc agccacctgg
     1021 ccatctctcc gattcgagga gcaatgactg ccttgcccga tgccatgtat tccctcagct
     1081 tgagtggtga gtagtggaac gatccggttc cgcgatcagt gattaccgcc acatccatgg
     1141 ccgccacgtg ctcgggaatc tcgagatagc caactgtccc ggtcatcacg acgttgcgga
     1201 ggccgaggtc gcgcacgctg tcttcgacat gtgcccgctc gaggccatcg ccgacgagga
     1261 ggagagtcgc ctctggtatc tccgtctcga tccgcttcat ggcctccagt gccatgtcca
     1321 gcccgtgaaa cttgcgaaag ctgcccaccc agccgatgac gaagcgttcc tcgagcccga
     1381 ggcgacgcct gacctcgtga ccgtgccgct ccggggagaa catctcggga tcgaccccgt
     1441 tcggtgtcac cagcaaacgc tccccgggta ccccgagccg accgacctgc tcggctatct
     1501 cgaccgagcc gcaagcaacg acatcggcag ccctgagcaa cggagcctcc gcggagcgct
     1561 cgaggatgcc ctgccaaccg ggtctcttga ctccccagcc ggcggcttcc tggactatga
     1621 gcgcaggggt gaagaccacg agtgggcgct tcaacgccct ggccgcggtc accccggcgt
     1681 cgtgaaacag gtcgtggcgc tgccacacga atctcacgtc ttccgaccgc caggggccgt
     1741 ccaacccggc ctctctgaac tggcgagcct ccagagcctt gcgtgcgtct ttgatcccgg
     1801 tcttgaccgt ggtcggcacc ttgcgtcgta gcgagggtgg gcccggtggg cccttcagcg
     1861 tcaccggatc ggatgcccta cgcattatct cctcgggctc gaggacgccc tcgggagtga
     1921 cgatccagga cttcccccag agcctcctcg cagccgccgc ccagctggca acactgatcc
     1981 aggaggcgac cggtgccgtc tggccacggg cccgatttgg gttcagaaag agcactccac
     2041 ccgtgctcac gcgtctgttc gctcaatgcc gggtgggaaa ttcacgggcc gtattctggc
     2101 atccatgccg gcgattgaag tcgtgaccat tctggctgtc ggccgacatg cctgagaccg
     2161 cagagggccg ggccttccgg gttgcacacc tcaccacggt ggatctcagt cttcgttttc
     2221 tcgttcggcc ccagctactc gcggtcaacg aggcaggagg cgaggcggtc ggtgtgagcg
     2281 ccccgggtcc ctgggtggag gagctcgagc gggacggcat ccggcacgtc gctctgaccg
     2341 cttcgacgag ggggatgagc gtgggcaacg atctcaaggc gatcatccag ctctggagag
     2401 tgcttcggac ggaggagttc gccgtgctcc atacccacaa cccgaagcca ggtgtctatg
     2461 gacggatcct cggtcgtctt gccggtgtgc cactggtggt gaacaccttg cacggtcttt
     2521 atgcgaccga ggacgacacg ccggtcaagc gcggcgtggt ctatgcactc gaggcggtcg
     2581 ccgcccgatt ctccgacgtg gagctgcatc agaacgagga ggatctcgcg ttctgccgcc
     2641 gcacccgaat cctccctgcc ggcaaaggcc agctcctcgg caatggcgtc gacctggtga
     2701 ggttcgaccg ggacaaggtc gatcccggtg cccgcacccg actcagggcc gagttcgggg
     2761 ccggagacag tgatgtcgtg gtggggatgg tgggccgtct cgtggccgag aagggctatc
     2821 ccgagttgtt cgaggccgcc acgaagctgg gtgagggtta tgtggtcgtg gtcatcgggc
     2881 cagacgaccc tgagaagtcg gatgccctgc cgcccgatgc cgtcgccaat gcccaagcgg
     2941 cgggagttcg ctttctcggc atgcgtaccg atgtggagga tctctactcg gcgatggatc
     3001 tcttcgtcct gccctcgcac cgggagggct tcccaagagc ggccatggag gcggccgcca
     3061 tgggcttgcc gatcatcgcc accgacatca ggggatgtcg tcaggtcgtc gacgacggcg
     3121 tcaacgggct tctcgtccct gtgctcgacc cggacagact gggagaagcc atcaggaagg
     3181 taggcgacga cccgacacgt cgagagaaga tgtcggaggc cagtgcccgc atcgctcgtg
     3241 agcgcttcga cgagcaaagg gtggtcgaca tcgtcatgga cgcctaccgg gacgggctcg
     3301 tcgccaaggg cctcggccat ctgatgccac cggcgatgat tgcccccgcg ccacgtcggc
     3361 agatgcggct cgcgacgtcc cgtgatgctc gagccctagc cgagctccat acgtcccaga
     3421 tcgcaaccgg attcctgcct cttctcggcc gcgggttcat gaatgtcttg tatcaggcgc
     3481 tgatcgcctg gaagggagca gtggtcgtgg tcgtcgacga cggcggcggg ccggtcggct
     3541 tcacggcagg agtggagaac gtgggcgagt tctactcaca cttcgccagg cactacggat
     3601 ggcgggccgg catcgcggcc ctgccccggt tgatcaggcc aagcaacctg cgccgggcct
     3661 gggagacgtt ccgatacggc cagaatcagg tggacgtgcc ggcggagttg ctctcgatgg
     3721 tggtggcccc gagagctcgg ggaaagggcc tctcgctcat gcttggggcg cgacttctcg
     3781 atgacctgag ccgacggggt gtccccgccg tgaaggtgac agtcggctcc gagaacgaga
     3841 cggccatcag cgtctatcga aagatgggct tcaaggacac cgagcgaatc caggttcacg
     3901 ccggcgagag ctctgaggtg ctcgtttggc ggctctgata gttgcgggtg tcgtcagcct
     3961 cgcctggtgt ttcttcgcga tctggttggg gcccgtcttc ggctatgtcg atcggcccga
     4021 cgactcggcg ttgacagcac acgagcgccc tgctgttccg ttgggcgggg tgggtgtctt
     4081 cctgggggtc aatgcggccg gtttcatgcg gagcgatctg gagctcgggc tcctcatcgc
     4141 aacgacgatg gtcttgatac tgggtctcat cgatgaccgg agggggatcg atccaaagat
     4201 gcggctcggt gttgaactgg tggccgcggt cgtgctggtg gtcaatgttg ccggcgaccc
     4261 cggcatcgtg tacgtcgttc ttggcatcgc gctcgccgtg ttcgccatca acagcgtcaa
     4321 cctgttcgac ggcctcgacg gattggttgg ttcggtgggg ctggtgacgg ctgcgggctt
     4381 ggccgtgatc gcccaaggcc gtggtctcga tttcgtgtcg cccctggagc tgggggcggc
     4441 attggccggc ttcctggttc tcaactggca cccagcccgg gtgttcatgg gcgatgccgg
     4501 cgcctacttc ctaggtctgt acctgacgca cttgatcatc gactcgacca acgattctgc
     4561 gttggagctg gtgctcacgg ccggcgtgct gggagtgttc gccatcgaca tgctggtgac
     4621 ccttctccgc cgccgtctga atcatcgccc cctgttcctc ggtgaccgca gccacatcta
     4681 cgaccagctc cgtgaccggg ggttgggggt tcggtcgatc gtcgtgatcg ccgccaccac
     4741 ccaggcgatt ctgcttgcct tcgtgatcgc cgccgagcgt cttctcgggg tcgaatgggg
     4801 tctggtgacc ctcagtggcc tccttgccct gcttctggcg gggctggccc gactcgggtt
     4861 cctcagggtc gaccaagctc gaacctgacg tctcgggact cgatcagata gagactcgac
     4921 ctggtgtgct caacgcaatc tgaagcgatc gaaaggcctc
//
LOCUS       DASTXO010000330         2305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_352128, whole genome shotgun sequence.
ACCESSION   DASTXO010000330 DASTXO010000000
VERSION     DASTXO010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352128"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VFU96_07025"
     CDS             complement(<1..873)
                     /locus_tag="VFU96_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5113057.1"
                     /translation="MNVPLPEPESLGNGIHLIPAPLPFKSPAWVNTYAVEADGGILLL
                     DCGTDWEPGRDAIHDGFQRLGLDESAVHTLVVSHLHLDHVGMSARLVAELGCRFVMHE
                     RAAKLVDRYNDTPAYVDRLLRIVRTHGVPARVLGSVTGDLATRPDYMPPIERPDHLVA
                     DGERIDIGDGRWLEVIHTPGHEPAHICLRDSRTGILFSGDHVLPRISPVIMWDEDLGD
                     PLGNYLASLQKLLGMNPGITYPAHGTLIERGDERARQILLHHDRRLLDMAELVRSRDS
                     TAWEVMLKSFRPNLT"
     gene            complement(892..1383)
                     /locus_tag="VFU96_07030"
     CDS             complement(892..1383)
                     /locus_tag="VFU96_07030"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEU5113058.1"
                     /translation="MDEPILTDHEIMTLEAQPTLAVRVRGPMDELDLGAMFATHLPRL
                     HAAAPGAGPAYARYHEFGPSHADIEIGVPIGDISPDLPDPEGGLDPIEPSELPGGLTA
                     VVIHHGSYETLGAAYDMFHDWIHSQGHEDGPGAWESYIEVPDDVEDVASLRTVLHWPL
                     EEE"
     gene            complement(1376..1600)
                     /locus_tag="VFU96_07035"
     CDS             complement(1376..1600)
                     /locus_tag="VFU96_07035"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEU5113059.1"
                     /translation="MSSITKTIRLSGQAADSIEDAVRTVLARAASTVSDIQSFRIVEV
                     GGSVDDSGVPTKFSVTLDITFGIKDPVEHG"
     gene            1686..>2305
                     /locus_tag="VFU96_07040"
     CDS             1686..>2305
                     /locus_tag="VFU96_07040"
                     /inference="COORDINATES: protein motif:HMM:NF016013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HEU5113060.1"
                     /translation="MVKVRGQRRTLPELSEELFLDIGDSATKRWRFAFLLLLAAVIAT
                     TGLLADSTATVIGAMIVAPLGVPIIGTALGIVIADGRRLVASAVTVMLGGAAVVAIGW
                     FLAAVLPEIVPITRNSQVTSRTSPNLIDLVTAVATGFAGAFGLARKDVSDVMPGVAIA
                     ISLVPPLAVVGITLQAGDPNSAAGAFELFASNMLAMIVAGSILFTA"
BASE COUNT          396 a          710 c          753 g          446 t
ORIGIN      
        1 ggtcaggttg ggccggaagg atttgagcat cacctcccac gccgtggaat cgcgggatcg
       61 gaccaactcg gccatgtcga gaagacggcg gtcgtggtgg agcaggatct gtcgtgccct
      121 ctcgtctccc cgttcgatga gagtgccgtg cgccggatag gtgatcccgg ggttcattcc
      181 gagcagcttc tggagtgagg cgaggtagtt cccgagcggg tccccgaggt cttcatccca
      241 catgatcacc ggcgagatcc ggggcaacac gtggtccccc gagaacagaa tccctgttct
      301 cgagtcacgc aggcagatgt gggccggttc gtgccctgga gtgtggatca cctcgagcca
      361 gcgcccgtca ccgatgtcga ttcgctcacc atcggccacc aagtgatcgg gccgttcgat
      421 cggcggcatg taatcggggc gggtggcgag gtctccggtc accgacccca agacgcgtgc
      481 cggcacacca tgggtccgga cgattcggag cagccggtct acatacgccg gcgtgtcgtt
      541 gtaacgatcg accagcttgg cggcccgctc gtgcatcacg aatcggcaac ccagttccgc
      601 gacgaggcgg gccgacatgc cgacatggtc gagatggaga tgcgagacga ccagcgtgtg
      661 cactgccgac tcgtccagac cgagccgctg gaacccatcg tggatcgcgt cacgtcctgg
      721 ttcccaatct gtcccacagt cgaggagcag gatcccgcca tcggcctcga cggcgtaggt
      781 gttgacccag gccgggctct tgaaagggag cggggccggg atcaggtgga tgccgttgcc
      841 gagtgactcc ggctcgggca gcggcacgtt catcgccggc gtctcaccgg gtcattcctc
      901 ttcgaggggc cagtgcaaca ccgtccggag actcgccacg tcctccacgt cgtcgggaac
      961 ttcgatgtat gactcccaag ccccaggacc gtcttcgtgt ccctgggagt ggatccaatc
     1021 gtgaaacatg tcgtaggcgg ccccaagcgt ctcgtagctc ccgtgatgaa tgacgacagc
     1081 ggtcaaaccg ccggggagct cggagggctc gatcgggtct aggccacctt cgggatcggg
     1141 gagatcgggg gagatgtcgc cgatcgggac tccgatctcg atatcggcat gggagggccc
     1201 gaactcgtga tatcgggcat acgcaggtcc agccccgggt gcggcggcgt gcagccgagg
     1261 gagatgcgtg gcgaacatcg ccccgaggtc gagctcatcc attggtcccc gaactctcac
     1321 cgcgagagtg ggctgcgcct cgagggtcat gatctcgtgg tcggtcaaga tcggctcatc
     1381 catgttcgac cgggtccttg atcccgaagg tgatgtcgag ggtcacgctg aacttggtgg
     1441 gcacaccgga gtcgtccacc gagcccccga cctcaacgat cctgaagctc tgtatgtccg
     1501 agacggtcga ggcggcccgg gccaagactg tcctgacggc atcctcgatc gagtcggcgg
     1561 cctggcccga caacctgatg gtcttggtga tggatgacat tgctacttcc gattttcgaa
     1621 gctagccggg tggtacggtc cccgccgtgg atggcgctcc tgatattcgt cggggacgcg
     1681 gtgccatggt gaaggtgcga ggacagcgtc gaactctgcc tgagctcagt gaggagctct
     1741 tcctcgacat tggcgactcc gcgaccaaac gctggcgatt cgcgttcctt ctcctccttg
     1801 ccgctgtcat cgccaccacc ggattgttgg cggattcgac tgcaaccgtc ataggagcca
     1861 tgatcgtcgc ccctctcggg gtcccgataa tcgggacggc tctcgggatc gtgatcgcag
     1921 atggcaggag actggtcgcc tcggctgtga cggtcatgct gggtggggcc gccgtggtgg
     1981 cgatcggctg gtttctggct gccgtcctac ccgaaatcgt tccgatcaca cgcaacagtc
     2041 aggtgacgag tcggacctca ccaaatctga tcgacctcgt gaccgcagtc gctaccggct
     2101 tcgccggggc gttcggattg gcaaggaaag acgtgtcgga tgtgatgccc ggagtagcca
     2161 tcgccatttc gctggttccg ccactggccg tagttggcat cactttgcaa gccggcgacc
     2221 cgaacagcgc agcgggcgcc ttcgagctct tcgcctccaa catgttggcg atgatcgtgg
     2281 ctggatcgat cctcttcact gccta
//
LOCUS       DASTXO010000331         2362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_646539, whole genome shotgun sequence.
ACCESSION   DASTXO010000331 DASTXO010000000
VERSION     DASTXO010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646539"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VFU96_07045"
     CDS             <1..709
                     /locus_tag="VFU96_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113061.1"
                     /translation="GKKWISTTYTMVLLRRMGLPRSDSRALKSCQLFLKEGLGTRRNS
                     ETCVSAMVLALLYWFDVEDRKREPVLEHLLGKQLKDGGWNCEIRSSHGSFHTTSSALE
                     ALRECVVAGAPGINEIEAAEARGREFFLEHRMFRSHRSGDIVNQRFLRFSFPPRWHYD
                     ILRGLDYFWSAGALSDARLEDAISVLVEKQDRDGRWPLQQRWPGETWFEMETVGEPSR
                     WNTLRAMRVLDGWSGEK"
     gene            721..1179
                     /locus_tag="VFU96_07050"
     CDS             721..1179
                     /locus_tag="VFU96_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEU5113062.1"
                     /translation="MHRILASLFGAGLILRKVRGSDAGSGTIGGLVALPFALLVGETW
                     GWPGQLVATVLVTAAAIWASAGVVESEGDAGWIVIDEAAGVFVATIGLLGWAAVVGFV
                     LFRIVDIFKRPFPGVRQADSLPGATGIVLDDLVAGAWALAMAHLVNATLM"
     gene            1234..1674
                     /locus_tag="VFU96_07055"
     CDS             1234..1674
                     /locus_tag="VFU96_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5113063.1"
                     /translation="MDWNPKAIVVGVDGSETSTRAAGIATEIARSRQAKLLLVTVVRP
                     PEGWWGIGGAPPTPEALSAALVEGQQQVIRETEEQLSLDGMDYETVEELGDPTSRLIA
                     VAEEREAGLIVIGKRGAGLAERVMLGSVADRLCHHSPVPVMVVP"
     gene            1700..2287
                     /locus_tag="VFU96_07060"
     CDS             1700..2287
                     /locus_tag="VFU96_07060"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2064 domain-containing protein"
                     /protein_id="HEU5113064.1"
                     /translation="MPVVAILPVKSFRLGKGRMADHLTDDDRSTLGRALAERTAALAV
                     DAGLIPILVAGDGDVGEWALMNGFPSIPDPGEGLDGAALAGTEWATEASSEWVVLHTD
                     LPLLTASELEELHESLARAGTVIAPSADGGTSALGGEGPANFSYGPGSFHRHLGRFPA
                     AAIVARPGLLHDIDTFADLESAREHPRGAWLAHLR"
BASE COUNT          384 a          772 c          812 g          394 t
ORIGIN      
        1 cggcaagaag tggatctcga ccacctacac gatggttctg ctcaggcgga tgggcctccc
       61 acgctccgac tcgagggctc tcaagtcatg tcagctcttc ctgaaggagg gcctggggac
      121 ccgtcgcaac agcgagacct gcgtctctgc gatggtcctc gccctgctct attggttcga
      181 tgtcgaagac cggaaacgag aaccggtgct cgagcatctg ctcggcaagc aattgaagga
      241 cggtggatgg aactgcgaga tacggtcgag ccacggctca tttcacacca cgtcgtccgc
      301 cctcgaggca cttcgcgagt gcgtggtcgc cggcgccccg ggcataaatg agatcgaggc
      361 ggccgaggct cgggggcggg agttcttcct cgagcaccgg atgttccgat cccaccgaag
      421 cggggacatc gtcaatcagc ggttcctccg gttctcattc ccaccccgct ggcattacga
      481 catcctgcgc gggctcgact acttctggtc ggccggcgcg ctgagcgacg cccgtctcga
      541 ggacgcaatc agtgtgctgg tcgagaagca agatcgcgac ggccgatggc ccctccagca
      601 gcgctggcct ggcgagacct ggttcgagat ggagaccgtg ggcgagccga gcagatggaa
      661 caccctgagg gcgatgcgag tcctcgatgg ctggagcggc gagaagtagc ctcgccgccg
      721 atgcaccgaa tcctcgcttc tctgttcggg gcaggcctga tcttgcgcaa ggtccgaggc
      781 tcggatgccg ggtcgggaac gattggcggt ctggtcgccc tcccgttcgc tctcctcgtc
      841 ggcgagacgt gggggtggcc gggacagctg gtggcaaccg ttctcgtcac cgccgcggcg
      901 atttgggcgt ccgcgggcgt tgtggaatcg gagggagacg ccggctggat cgtgatcgac
      961 gaggccgccg gagtcttcgt cgccacgatt ggcctgctcg gatgggcggc agtggtgggc
     1021 tttgtcttgt ttcgcatcgt ggacatcttc aaacggccct tcccgggggt ccgacaggcg
     1081 gactcgctgc ccggcgccac gggcatcgtg ctcgacgatt tggtcgccgg agcctgggcg
     1141 ctggccatgg cgcatctcgt caacgccacc ctcatgtgac ccggccgaca gcgaccaacc
     1201 tgcgtatggt ctgctgagga ggtatcagag cagatggatt ggaacccgaa agcaatcgtc
     1261 gtcggcgtcg acggctcgga gaccagtacg cgtgcagccg ggatcgccac cgagatcgcc
     1321 cgctcccggc aggcaaagct cctgctggtc accgtggtgc gaccgcccga gggatggtgg
     1381 ggcataggtg gcgccccgcc cactccggag gcactctcgg ccgccctcgt cgaaggacaa
     1441 cagcaggtca tcagggagac cgaagagcag ctctcactcg acgggatgga ttacgagacg
     1501 gtggaggaac tgggcgaccc cacgtcccgc ctcatcgccg tggccgagga gcgcgaggca
     1561 gggctcatcg tcattggcaa gcggggagcc gggctggcgg agcgggtgat gctcggaagc
     1621 gtcgccgatc gcctctgcca ccattcaccg gtgccggtga tggtggtgcc ctgacccagg
     1681 gacaaccccc accagccccg tgcccgttgt cgccatcctc ccggtcaagt cgtttcgact
     1741 gggcaaaggg cggatggcag accacctcac cgacgacgat cgatcaacgc tgggccgcgc
     1801 cctcgccgag aggaccgcgg ccctggcggt cgatgccgga ttgatcccga tcctggtcgc
     1861 cggtgacgga gatgtcggcg aatgggcact gatgaatggg ttcccatcga tacccgaccc
     1921 cggtgagggc ctggatgggg cggcgctggc ggggaccgaa tgggccaccg aggcgtcgag
     1981 cgagtgggtc gtcctccaca cggatctccc gctcctcacc gcatccgagc tcgaggagct
     2041 gcatgagtcg ctggccaggg cggggaccgt catcgccccc tcggccgatg gcgggacctc
     2101 cgcccttggg ggggaaggtc cggcgaactt ctcgtacggc cccggcagct ttcatcgcca
     2161 tctcggcaga ttcccggcgg cggcgatcgt cgccaggccg ggcctcctcc acgacatcga
     2221 caccttcgcc gacctcgaat ccgctcgtga gcacccgcgc ggggcctggc tcgctcatct
     2281 gaggtgacca accgcttcga ccccagggtg ctggccccag accccgaggc cagagcccag
     2341 gtgctgaggc ggctggaggc cg
//
LOCUS       DASTXO010000332         2130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_757201, whole genome shotgun sequence.
ACCESSION   DASTXO010000332 DASTXO010000000
VERSION     DASTXO010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757201"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VFU96_07065"
     CDS             complement(<1..738)
                     /locus_tag="VFU96_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU5113065.1"
                     /translation="MTAQTDYTDATRATGEAIIELEGVDKFFGDFQALKGIDMRVGRQ
                     EVVVVIGPSGGGKSTLIRCINRLEKHDGGRVAVDGIDLSDDVRNIQEVRRETGMVFQQ
                     FNLFPHLTVLDNVTLAPRQVRKLPKKEAEDLAMQMLTRVKIPEQARKYPGQLSGGQQQ
                     RVAIARALAMQPKVMLFDEPTSALDPEMIKEVLDVMVELARGGMTMIVVTHEMGFARA
                     VANRVVFMADGEIVEVGSPEHFFTNPQE"
     gene            complement(735..1982)
                     /locus_tag="VFU96_07070"
     CDS             complement(735..1982)
                     /locus_tag="VFU96_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU5113066.1"
                     /translation="MTVDAPVFDETALEVEPARGPMDWVRKNLFNRWYNVVLTLVLSA
                     LLVWAVVALARGLLGADFEIIRVNLRLFMIGDFPGDQLWRPWTATYLIATLIGVVAGS
                     VAAGARDRANEAGLPLVRTSLRQVVFRFWPLLALVIVILAFTTTITPALLTVGAVGVG
                     VGSYFLGRVMPRAVRRWTWLIALLLLFSAYVALAGFGGVGWEGWGGLQLNLFLTVAGI
                     MFAFPLGLLLAMGRRSSLPVIRTISVGYIEFVRGVPLITLLLAGAFALGFLIPSDLRP
                     GLVTRMLIAITAFEAAYIAEVVRGGFQAVPHGQVEAAQAVGLAPWKTMRLIVLPQALR
                     ATIPAMVGQFISLFKDTTLVAILGVTELLDATFIVNSQPAFLGQGLFVLTLGFAALVF
                     WVGSYTMSRESRRLERRLGVGER"
BASE COUNT          436 a          705 c          650 g          339 t
ORIGIN      
        1 ctcctgtggg ttggtgaaga agtgctcggg ggaaccgacc tcgacgatct ccccgtctgc
       61 catgaacacc acccgattgg ccacggctcg ggcgaagccc atctcgtggg tgacgacgat
      121 catcgtcatc ccgccccggg ccagctcgac catgacatcg agaacctcct tgatcatctc
      181 ggggtcgagc gccgaggtgg gctcatcgaa gagcatgacc ttcggttgca tggccagggc
      241 ccgggcgatg gcaacccttt gttgctggcc ccctgacagc tggcctgggt acttgcgggc
      301 ctgctcaggg atcttgaccc tggtcagcat ctgcatggcc agatcctctg cctctttctt
      361 gggcagcttg cgcacctggc gcggggcgag agtcacgttg tcgagaaccg tcagatgggg
      421 aaagaggttg aattgttgga agaccatgcc tgtctcccga cgtacctctt ggatattgcg
      481 cacgtcgtcc gagaggtcga tgccgtcgac cgccacccgc ccgccgtcgt gcttctcgag
      541 acggttgata caccggatga gagtcgattt gccacctccc gagggcccta tgaccaccac
      601 cacctcctgg cggccaaccc gcatgtcgat ccccttcagc gcctggaaat ccccgaagaa
      661 cttgtccacc ccctccagct cgatgatggc ctcgccggtg gcccgggtcg catccgtgta
      721 atcggtctga gcggtcatcg ctcacccacc cccaatctcc tctccaatcg ccgggactcg
      781 cgcgacatgg tgtaggagcc gacccagaag acgagggcgg cgaatccaag ggtcagcacg
      841 aacaggccct gtccgaggaa ggcaggctgg gaattgacga tgaaggtcgc atccagcagc
      901 tcggtgacac cgaggatcgc caccagagtc gtgtccttga acaggctgat gaactgcccg
      961 accatcgcgg ggatcgtcgc acgaagcgcc tgtgggagca ctatcaggcg catcgtcttc
     1021 cacggagcga gcccgacggc ctgggccgcc tccacctggc cgtgaggaac tgcctgaaaa
     1081 cctcctctca ccacctcggc gatgtatgcc gcctcgaacg cggttatggc gatgagcatc
     1141 cgggtgacca gacccggtct caggtcactc gggatgagga aacccagggc gaaggcgccg
     1201 gcgagaagca acgtgatcag gggcactcct ctgacgaact cgatgtatcc caccgagatg
     1261 gtgcggatca ccggcagcga cgaccgcctt cccatcgcca ggagcagacc gagggggaag
     1321 gcgaacatga tcccggcaac ggtcaggaac aggttgagct ggaggccgcc ccagccctcc
     1381 caacccacgc caccaaaccc ggcaagggcg acataggcgc tgaagaggag caagagggcg
     1441 atcagccaag tccatctccg caccgcccgg ggcatcaccc gtcccaggaa atacgaccca
     1501 actcccaccc cgacggcgcc aacagtcagg agcgccgggg tgatcgtcgt cgtgaacgcc
     1561 aggatgacga tcaccagcgc caggagcggc caaaaacgga agacaacctg gcggagcgag
     1621 gttcggacca agggaagacc cgcttcgttg gcccggtcac gggctccggc ggccacggac
     1681 ccggcgacga cgccgatcag cgtcgctatg agatatgtgg ccgtccaggg ccgccacagc
     1741 tggtcgccgg ggaagtcccc gatcatgaac agcctcagat tgacccggat gatctcgaag
     1801 tcggccccca gcaggccacg ggccagcgcg accaccgccc acaccaggag cgcggacagc
     1861 accagggtga gcacgacgtt gtaccacctg ttgaacaggt tcttccgaac ccagtccatc
     1921 ggcccccgcg ccggctccac ctcgagggcg gtctcatcga agactggtgc gtcgacggtc
     1981 atcgggtcac caactgcaga cgacgattga tcagattgac gatcaaggag atgaccagtg
     2041 agaacaccag atagatcccc atcaggattg cgatcatctg tggggcgggt tgtccgttgc
     2101 cgatcatctg gaacgtcaac cgcgtgacct
//
LOCUS       DASTXO010000333         2781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_684035, whole genome shotgun sequence.
ACCESSION   DASTXO010000333 DASTXO010000000
VERSION     DASTXO010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684035"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VFU96_07075"
     CDS             <1..424
                     /locus_tag="VFU96_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEU5113067.1"
                     /translation="LYINSPGGSITSLFAIYDTMQYIKPDVNTVCMGMAASAAAVILA
                     GGAEGKRYALPHARVMLHQPHGGAQGQASDIEIQARLIVQMREQLNQILAEHTGQEYE
                     KIAVDTERDYWMLADAAKEYGVIDGILTRRELAAVASK"
     gene            441..1703
                     /gene="clpX"
                     /locus_tag="VFU96_07080"
     CDS             441..1703
                     /gene="clpX"
                     /locus_tag="VFU96_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003938129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEU5113068.1"
                     /translation="MARLGDGGELLKCSFCGKSQKQVKKLIAGPGVYICDECIDLCNE
                     IIEEEFSATEEITFTELPKPVEINSYLDDYVVGQEDAKKKLSVAVYNHYKRIRGGEVQ
                     RDVELQKSNILLIGPTGSGKTLLAQTLARQLNVPFAIADATSLTEAGYVGEDVENILL
                     KLLQAADYDVNRAQQGIIYIDEIDKVSRKAENPSITRDVSGEGVQQALLKILEGTVAS
                     VPPQGGRKHPHQEFIQLDTTNILFICAGAFGGLEDIIANRVGRRGVGFGAEVRGKADG
                     RPSDLLNQVMPEDLMKYGLIPEFIGRLPVMATVEDLDMDALIRILTEPKNALTRQYAR
                     MFEMDDVELVFTPDSLDAIAAQAIKRGTGARGLRAILEEVLLNTMYDLPSRTDVERVV
                     IDRDVVAEKVNPTLVPAREEDGPAEKSA"
     gene            1940..>2781
                     /locus_tag="VFU96_07085"
     CDS             1940..>2781
                     /locus_tag="VFU96_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113069.1"
                     /translation="MRVSVRRVRFVVALMMALMLLASLSIVLSGAAAATTGQQSNSDH
                     QGEDCTDFDLKSDGSGDHEDCDSEECPDLAVQSSSGDDSKDCEPDGEECPGIALQTSG
                     GDHEDCDSEECPDLAVQTGGGDHDDCDSEECPDVTIQGGGDHDGDDEDCTSTTTTTTT
                     TVPTVAPPTTVPGLDLAAAGAECISDIPYLRYEIDYPPGQSATITFLNPTGPDVVYED
                     VPLSGAVLWPGASENPPDWPGWVREDGVWVEADDGFLWARGTISVLFEVNPSTMVTVS
                     YQQGT"
BASE COUNT          571 a          828 c          887 g          495 t
ORIGIN      
        1 cctctacatc aactcgccgg gaggatcgat cacgtccttg ttcgcgatct acgacacgat
       61 gcagtacatc aagccagacg tgaacaccgt gtgcatgggg atggccgcct cggccgccgc
      121 cgtcattctc gccggtgggg cggagggcaa gcggtacgcg ttgccccacg ctcgggtcat
      181 gctccaccag cctcatggcg gcgctcaggg ccaggcatcc gacatcgaga tccaggcccg
      241 cctcatcgtc cagatgcggg agcagctcaa ccagatcctt gctgagcaca ccggacagga
      301 atacgagaag atcgcggtgg acaccgaacg ggactattgg atgctcgccg acgcagccaa
      361 ggaatacggg gtcatcgacg gaattctcac ccgtcgggag ctcgcggcgg tagcctccaa
      421 gtaaggaggt tcacaactcg atggcacgac tgggtgacgg cggtgagctg ctcaagtgca
      481 gtttctgcgg aaagtctcaa aagcaggtca agaaactcat cgctggaccg ggcgtctaca
      541 tctgtgacga gtgcatagat ctctgcaacg agatcatcga ggaagagttc tccgctaccg
      601 aggagatcac cttcaccgag ctgcccaagc cggtcgagat caactcctat ctcgacgact
      661 acgtggttgg tcaggaggac gccaagaaga agctgtcggt cgctgtctac aaccactaca
      721 agcggatccg gggcggcgag gtccagcgtg atgtcgagtt gcagaaatcg aacatcctgc
      781 tcatcggccc caccgggtcg ggcaagacac tcctcgccca aaccctcgcc cgccagctca
      841 acgtcccatt cgccatcgcc gacgcgacca gtctcaccga agccggctac gtgggcgagg
      901 acgtcgagaa catcctcctc aagttgctcc aggccgccga ctatgacgtc aatcgggccc
      961 agcaggggat catctacatc gacgagatcg acaaggtctc ccgcaaagcc gagaacccat
     1021 caatcactag ggacgtgtcg ggtgagggcg tgcaacaggc gctcctcaag atcctcgagg
     1081 gcactgtcgc ctcggtaccc ccccaagggg ggcgcaagca cccccaccag gaattcatcc
     1141 agctcgacac gaccaacatc ctcttcatct gtgccggagc cttcggtggg ctcgaggaca
     1201 tcatcgccaa ccgagtggga agacgtggcg ttggctttgg agcagaggtc cgtggcaagg
     1261 ccgatgggcg gccgtccgac cttctgaacc aggtgatgcc cgaggatctg atgaagtacg
     1321 gcctgatccc cgagttcatc gggcgtctgc cggtgatggc caccgtggag gatctcgaca
     1381 tggacgcgct gatccgaatc ctcactgagc cgaagaacgc cctgactcgt cagtacgcac
     1441 gcatgttcga gatggatgac gtcgagttgg tgttcacacc cgattcgctc gacgcgatcg
     1501 ctgcccaggc gatcaagcgt gggaccgggg cacgagggct acgagccatt ctcgaagagg
     1561 tccttctcaa cacgatgtac gacctccctt ctcgtacgga cgtggagaga gtggtcatcg
     1621 atcgcgacgt cgtcgccgag aaggtaaacc ccacactggt gcccgcccgc gaagaggacg
     1681 gtcccgccga gaagtcggcc tgacgtcatc gcccactctg ggtgaggtga ctagcgcatt
     1741 cggactagtt ccgacagttc gtctcattga cactggcggg taacccgcgc cagttagggt
     1801 gtcgtggatc ctcctttggt gggtcataag gggaaaaagg gaggctttga ccccacggcc
     1861 ttttaggcgg tcacctgggt cgtgggttag ccagtggtga gaccggcccg tcccaacgcg
     1921 caatgaaggg atgggattca tgcgggttag cgtgcgacgg gttcgcttcg tcgtcgcgtt
     1981 gatgatggct ctgatgctgc tggcctcgtt gtcgatcgtt ctctcgggag cggcggcagc
     2041 aaccaccggg cagcaatcga acagcgatca ccaaggcgag gactgtacgg atttcgattt
     2101 gaagagtgat ggcagcggcg atcacgagga ttgcgattcc gaggagtgtc ccgatctggc
     2161 ggttcagagc tccagtgggg acgattcgaa ggattgtgag cccgacgggg aggagtgccc
     2221 cgggattgca cttcagacca gcgggggaga tcacgaggac tgtgattccg aggagtgccc
     2281 ggatctggcg gttcagaccg gcggtgggga tcacgacgat tgtgattccg aggagtgccc
     2341 ggacgtgacg attcagggtg gtggggatca cgacggtgac gacgaggact gcacgtccac
     2401 gacgacgacg accacgacca ccgtgcccac ggtggcacct ccgacaacgg ttcctgggct
     2461 ggacttggct gcggccgggg ctgagtgcat cagcgacatc ccatatctca ggtacgagat
     2521 cgactatccc ccggggcaat cggccacgat caccttcctc aaccccaccg ggcccgacgt
     2581 ggtttacgag gatgtgccgt tgagcggcgc cgtgctatgg cctggggcga gcgaaaatcc
     2641 ccctgattgg ccgggatggg tccgcgagga cggagtctgg gtggaagccg acgacggttt
     2701 cctctgggcc aggggaacga tcagcgtcct cttcgaggtg aacccgagca cgatggtcac
     2761 ggtctcctac caacagggga c
//
LOCUS       DASTXO010000334         2157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1199462, whole genome shotgun sequence.
ACCESSION   DASTXO010000334 DASTXO010000000
VERSION     DASTXO010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199462"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /gene="carB"
                     /locus_tag="VFU96_07090"
     CDS             complement(<1..529)
                     /gene="carB"
                     /locus_tag="VFU96_07090"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005961555.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEU5113070.1"
                     /translation="MPARRDLRSILLIGSGPIVIGQGCEFDYSGTQAAKVLRAEGYRV
                     ILVNSNPATIMTDPEFADATYIEPITPEVVAEIIAREQPDALLPTLGGQTALNVASDL
                     ARRGILDRLGVEMIGAGLEVIERAEDRGLFKETMEKVGLAVPRAGYARSMTEAVKIAH
                     EIGFPVMIRPSFILGG"
     gene            complement(513..1691)
                     /gene="carA"
                     /locus_tag="VFU96_07095"
     CDS             complement(513..1691)
                     /gene="carA"
                     /locus_tag="VFU96_07095"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU5113071.1"
                     /translation="MTTLDHALLCTADGETFRGRSVGAAGVTTGEAVFNTSMTGYQEI
                     LTDPSYAGQVVVMTSSHIGNYGVFAGDDQSSRPAVSGFVVRSLSRRDSNWRSQGALGE
                     YLRGHGVVAIADVDTRRLTRHIRDRGAMPVAVGVGVDEPELLEMAASAPSMVGQDLAS
                     SVTTPHAYTLDAEGERRGRVVAIDLGMKRDILANLTGRGFDVEVVPAGTNADHVLALS
                     PDGVFVSNGPGDPEPLETQIETLRGILGKVPLFGICLGHQVLGLAVGASTYKLPFGHH
                     GGNHPVRRLEDGKVEITAQNHGFAVDLWSLAGRPAPERSGLASPDLLPGVVDTGFGPL
                     VPTHQNLNDGTLEGMQLVDIPAFSVQYHPESAPGPRDARGLFDRFARMMEDEADAGQT
                     "
     gene            complement(1688..>2157)
                     /locus_tag="VFU96_07100"
     CDS             complement(1688..>2157)
                     /locus_tag="VFU96_07100"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5113072.1"
                     /translation="RLDSNLKMYPPLRGPEDRAALRAALREGVIDAVATDHAPHAPAE
                     KAVPFEEAPRGVIGLETAAAVAATHVLGDDQRAFFDRMSVAPARIAGLARQGRDVEPG
                     SPANLVLFDPRRRWVPGSFVSKSQNSPFAGVELTGRVLATIYEGRISYEGGLG"
BASE COUNT          381 a          724 c          692 g          360 t
ORIGIN      
        1 cgccaccgag gatgaaggag ggtcgaatca tgaccgggaa cccgatctcg tgcgcgatct
       61 tcaccgcctc ggtcatcgac cgggcgtagc cggctcgcgg gactgcgagg cctaccttct
      121 ccatcgtctc cttgaacagg ccgcgatctt cggcacgctc gatgacctcc aggccggccc
      181 cgatcatctc gacccccaat cggtcgagga tccctctgcg ggcaaggtcg gatgcgacgt
      241 tgagggcggt ttggccgccg agcgtgggga ggagggcgtc aggctgctct ctggcgatta
      301 tctcggccac cacctcgggc gtgatcggct cgatataggt ggcgtctgcg aactccggat
      361 ccgtcatgat cgtggccggg ttggaattga ccaggatcac ccgatatccc tcggctcgca
      421 ggaccttggc cgcctgagtc cccgagtagt cgaactcgca tccctgtccg atgacgatcg
      481 gcccggagcc gatgaggagg atggatctca ggtcacgtct ggccggcatc agcctcgtcc
      541 tccatcatcc gggcgaaacg gtcgaagaga ccacgcgcgt cgcggggccc gggggcggat
      601 tcggggtggt actggaccga gaacgcgggg atatccacca gttgcatccc ctccagcgtc
      661 ccgtcgttga ggttctgatg ggtcggcacc agcggcccga acccggtgtc gacgaccccg
      721 gggagcagat cggggctcgc caaacccgat ctctccggcg ccgggcggcc agcgagcgac
      781 cacaggtcca cggcaaaccc atggttttga gcggtgatct cgaccttgcc atcctcgagg
      841 cgtcggaccg ggtgattgcc accgtggtgc ccgaacggga gcttgtaggt gctggcgccg
      901 acggcaaggc cgagcacctg atgaccgagg cagatcccaa agaggggcac ctttccgaga
      961 atgccgcgga gcgtctcgat ttgtgtctcc aacggctcgg ggtcgccagg gccgttcgac
     1021 acgaacacgc catcgggaga cagggccagc acatgatccg cattggttcc cgccggtacc
     1081 acctcgacat cgaacccgcg tccagtcagg ttggcgagaa tgtccctttt catgccgaga
     1141 tcgatcgcaa ctacgcggcc gcgccgctcg ccttcggcgt cgagtgtgta ggcatggggc
     1201 gtagtgacgg aagaggccag gtcctgaccc accatcgagg gcgcagacgc cgccatctcg
     1261 agcaactcgg gctcatcgac cccgacaccg acggcgaccg gcatcgctcc ccggtctcgg
     1321 atgtgcctcg tcagacgccg ggtgtcgacg tcggcgatgg ccaccacccc gtgcccgcgc
     1381 aagtactcac ccagggcgcc ctgggatcgc cagttggaat ctcgcctcga caggctgcga
     1441 accacgaagc cggacactgc cgggcgagac gactggtcat cacccgcgaa cacgccgtag
     1501 ttgccgatgt gagacgaggt catgaccacg acctgacctg catatgacgg gtcggtcagg
     1561 atctcctgat atcccgtcat cgaggtattg aaaactgcct cgccggtggt gaccccggcg
     1621 gccccaaccg agcgtccccg gaaggtctcc ccatctgccg tgcacaacag agcgtggtcg
     1681 agtgtggtca accgagtccc ccttcatagg agattcgacc ttcgtagatc gttgccagca
     1741 ccctgccagt caactccacg ccggcaaatg gactgttctg tgatttggac acgaacgacc
     1801 cgggcaccca ccgacgtcgg ggatcgaaga ggaccaggtt ggcgggtgac cccggttcca
     1861 cgtcccgacc ctgccgcgcc agacccgcga tacgtgcagg ggcgacgctc attcggtcga
     1921 agaaggccct ctggtcatca ccgagaacgt gagtggccgc gactgctgct gccgtctcga
     1981 gcccgatcac tccgcgtggc gcctcctcga acggcaccgc cttctcggcg ggggcatgcg
     2041 gagcgtgatc ggtcgcgacc gcatcgatca cgccctcgcg gagcgccgcg cgcaacgccg
     2101 cacgatcctc gggcccgcgc aacgggggat acatcttgag attcgagtcg agtcggt
//
LOCUS       DASTXO010000335         2479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1457614, whole genome shotgun sequence.
ACCESSION   DASTXO010000335 DASTXO010000000
VERSION     DASTXO010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1457614"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="VFU96_07105"
     CDS             complement(<1..1009)
                     /locus_tag="VFU96_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006140051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEU5113073.1"
                     /translation="MKAVTVIPGDPGSLRLDDIPDPRPGRDDLLVSVLMAGICGTDHE
                     ILEGDLGYPPSGRDRLTLGHEALGRVRVAPDGSPFAPGDLVAPIVRRPDPVPCPNCAA
                     GEWDMCRNGRFTEAGIRGLDGYTAEMLALPVEFAVKVGTDPGLLGVLVEPASVVAKAW
                     EHIMHIGGRARWAANRVLVTGAGPIGLLAALFATRVADEIHVLDRAANGPKPGLVGDL
                     GAVYHDESLDRIGGGFDVVLECTGDPDLMMSVPGVTNPNGIVCLAGVADEGRELSVGS
                     DLVRSLVLENQTVFGTVNANRRHYEEAARVLAEASQPWLGRLISRRVPLSRFDEAYVR
                     HP"
     gene            complement(1006..>2479)
                     /locus_tag="VFU96_07110"
     CDS             complement(1006..>2479)
                     /locus_tag="VFU96_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015620829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEU5113074.1"
                     /translation="RVEMRMDLTIRFDYGHLVPWVTSRAGRLDAVGGPDALTLVTPVE
                     FHGENLSTVAEFAVSEGDRVPFVLTWHPAHRPAPKEVDAEEALEETEKWWRQWTDANT
                     YEGEWQDAVDRSLITLKALTYAPTGGIVAAPTTSLPEEIGGVRNWDYRFVWLRDATFT
                     LQTLLLAGHRREAIAWRDWLLRAVGGDPEDLQVMYGVAGERRLPELELGWLSGYEGSR
                     PVRIGNAAHQQIQVDVYGELMDAIYTARRLGMDHEETIWDLQQLLLENLEGKWVEPDH
                     GLWEVRGDPRPFTHSRLMSWVAFDRAVKCVEQWTFDGPVDHWRELRTEIRAEIEQQGY
                     DSERNTFTQSFGSKALDASLLLIPQVGFLPPTDERVLGTIEAIERELSINDNLIRRYD
                     PDEADDGLPGGEGAFLLCSFWMVDALAMADRGDEARRRFEFLLGLRNDVGLLSEEYEP
                     VSQRMLGNFPQAFSHLGLIDSAYNLQAEGRGPARDRASDS"
BASE COUNT          424 a          870 c          786 g          399 t
ORIGIN      
        1 ccgggtgacg gacatacgcc tcgtcgaatc ggctgagggg gacccgtcga ctgatcagcc
       61 gccccaacca gggctggctc gcctcggcca agacccgggc ggcctcctca tagtgccgcc
      121 ggttggcgtt caccgttccg aagacggttt gattctccag caccagactc cggaccagat
      181 cgctgccgac actcaattcc cgcccctcgt cggcgacccc ggcgaggcaa acgatcccgt
      241 tggggttggt cacaccgggc acggacatca tcagatcggg atccccggtg cattccaaca
      301 ccacgtcgaa ccctcctccg atccgatcca gggactcgtc gtgatagacg gcgccgagat
      361 caccgaccaa acccggcttg ggcccgttgg cggcccggtc gaggacatgg atctcatcgg
      421 caacccgggt ggcgaagagc gcggcgagga gcccgatcgg gcctgccccg gtcaccagca
      481 cccggttggc cgcccatcgc gcccggcccc cgatgtgcat gatgtgctcc caggccttgg
      541 ccaccacgct ggccggctcc accaggaccc caaggagccc cgggtcggtc cccaccttca
      601 ccgcgaactc gacggggagc gccagcatct ctgcggtgta accgtccagg cccctgatcc
      661 cggcctcggt gaatcggccg ttgcggcaca tgtcccactc cccggcggcg cagttggggc
      721 atggaacagg gtcgggccgg cgcacgattg gggcgaccag gtcgccgggt gcgaaggggc
      781 tcccgtccgg cgccaccctc acccggccca gggcctcgtg acccaaggtc agcctgtcgc
      841 gaccggacgg tggatagccg agatcccctt ccaggatctc gtggtcggtc ccgcatatcc
      901 ccgccatcag cacggagacg agcaggtcat cgcgaccggg cctcgggtcc gggatgtcgt
      961 cgagtcgtag tgacccggga tcccccggga tcacggtcac cgccttcacg aatcggaagc
     1021 ccggtcccga gccgggcccc ggccctcggc ttgcaggttg taggcggaat cgatcaagcc
     1081 gagatggctg aacgcctggg ggaagttgcc cagcatccgc tgcgatacgg gttcgtactc
     1141 ctcggagagg agccctacgt cgttgcggag cccgagcagg aactcgaacc gtcgtcgagc
     1201 ctcgtcccca cggtcggcca tcgccagggc gtcgaccatc cagaacgagc agaggagaaa
     1261 ggcaccttcg ccacccggca ggccgtcgtc ggcctcgtcg gggtcataac ggcgaatcag
     1321 gttgtcgttg atcgacagct cgcgctcgat ggcctcgatg gtgcccagca cacgctcatc
     1381 cgtgggaggg aggaaaccca cctgcgggat gaggagaaga gacgcgtcca gcgccttcga
     1441 cccgaaggac tgggtgaagg tgttgcgctc ggagtcgtaa ccctgttgct cgatctcggc
     1501 gcgtatctcg gttcgaagtt cccgccagtg atcgaccggc ccatcgaagg tccactgctc
     1561 gacgcacttc accgcccgat cgaatgcaac ccaactcatc aggcgggagt gagtgaaggg
     1621 tcgtgggtct ccgcgcacct cccacaagcc gtgatcgggc tcgacccatt tcccctctag
     1681 gttctccagg aggagctgct gcaggtccca gatcgtctcc tcgtgatcca tcccgaggcg
     1741 acgggccgtg tagatcgcat ccatcagctc cccgtatacg tccacctgga tctgctgatg
     1801 agccgcgttg ccgattcgaa ccggacggga gccctcatac cccgacagcc aacccagctc
     1861 cagctccggc agccggcgtt cgccggccac cccgtacatg acctgcagat cttcgggatc
     1921 gcccccgacc gcccgaagca gccagtcccg ccaagcgatg gcctcacgcc gatggcccgc
     1981 caggagcaac gtctggagag tgaacgtggc gtcgcgcagc cagacgaacc ggtagtccca
     2041 gttccggacc ccaccgatct cctcggggag ggacgtggtc ggggcagcga cgatgccacc
     2101 ggtcggggcg taagtgaggg ctttgagcgt gatcagtgac cgatcgaccg cgtcttgcca
     2161 ttcaccctcg taggtgttgg cgtcggtcca ctgtcgccac cacttctcgg tctcttcgag
     2221 tgcctcctcg gcgtcgactt ccttgggggc agggcgatgc gccggatgcc atgtgagcac
     2281 gaaggggact cgatctccct ctgacacggc gaactcggca accgtgctca ggttctcgcc
     2341 atggaactcg accggggtga ccagggtgag ggcgtcgggg ccgccgaccg cgtccagcct
     2401 ccccgcacga ctcgtcaccc agggcacgag atggccgtag tcgaagcgaa tggtgaggtc
     2461 catccgcatt tccacgcgg
//
LOCUS       DASTXO010000336         4050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1457620, whole genome shotgun sequence.
ACCESSION   DASTXO010000336 DASTXO010000000
VERSION     DASTXO010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1457620"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VFU96_07115"
     CDS             <1..334
                     /locus_tag="VFU96_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEU5113075.1"
                     /translation="GQLAGEIVDGPRKAAFLRSIAEAEGIPLSQVVAVGDGANDLDML
                     ATAGLGIAFNAKPVVKERADTAISVPYLDAILFTMGIRRQHVEEADEADPGFEVGERI
                     EVPGTPPV"
     gene            complement(341..1741)
                     /gene="gnd"
                     /locus_tag="VFU96_07120"
     CDS             complement(341..1741)
                     /gene="gnd"
                     /locus_tag="VFU96_07120"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HEU5113076.1"
                     /translation="MSGQADIAVAGLGVMGANLALNLADKGHRVGAFNRTASVTDEFM
                     AGEAGELGILPATSIEEMVGMLSRPRVVLIMVTAGKPVDAVIDSLLDHMEEGDIIIDG
                     GNSLYSDTERRQAFVESRGLLYLGMGVSGGEEGARHGPSIMPGGSRKAWEHVEPMLKS
                     IAAKAPDGRPCCEWIGTGGAGHFVKMVHNGIEYGDMQVIAEAYDLMRRGMGMTPMEMS
                     KVFAKWDEGRLRSYLIEITSEILTRELEGQPVVDVILDAAGQKGTGKWTVISSLDLGQ
                     PTTLVAEAVYARIVSSDPDARTRAAVIYPNAPHGLDEVTPDDVESALYASKIISYAQG
                     FRLMRAASDEYGWDLDLATIASIWRAGCIIRAAFLEDITAAFLRDPGLPDMMEDGFFS
                     KALLEAETSWRDVVARAALSGIPAPAYSSALAYFDGMRSERLPANLIQAQRDFFGAHT
                     FERVDGPRGEFFHHDW"
     gene            1793..2536
                     /locus_tag="VFU96_07125"
     CDS             1793..2536
                     /locus_tag="VFU96_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007271730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU5113077.1"
                     /translation="MTTRIGVDVGGSGVKAGVVDVEAGELVSERLRVDTPEPSTPPAV
                     AAAVTELVDQLDAPGRIGLGFPSVMRQGWVTTANNIDRSWIGVNALELFETAVGREIS
                     LLNDADAAGLAEVRYGAAKGVTGKVLVLTFGTGIGSALIVDGKLLPNIELGQLELGGV
                     RPAEKRYSAKSRRAENLSWDEWGARAREFIVLANEVFTPDLIVIGGGVSKFWDEFASH
                     VTFDDVKTTKAALANNAGIVGAAALAFSG"
     gene            complement(2540..3847)
                     /gene="hflX"
                     /locus_tag="VFU96_07130"
     CDS             complement(2540..3847)
                     /gene="hflX"
                     /locus_tag="VFU96_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014447000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEU5113078.1"
                     /translation="MTQEHASRRRRRLTATITDLEVAIQRAFLVGVELPGMTTVDSER
                     SLEELALLTDTAGSEPVDQELVRRTRIDPATYIGSGKAEEIANLTNALDIDVVVFDNQ
                     LTPAQQRNLQKLFGCDVVDREGLILDIFAQHAHTKVGALQVELAMNRYNLPRLRGRGT
                     SLSQQGAGVLARGPGETKLETDRRRILERNSKLERELKETVKHRATQRKARRKSDIAQ
                     ISIVGYTNAGKSTLLNALTDADVLVEDRLFATLDSTVRRLELPDQQTVLLADTVGFVR
                     RLPHHLVEAFRSTLSEVGEADLLLHMVDGTDPDPAGQIAAVREVLLEIGAAEIPELIA
                     INKADRVADHALTRLGNLYPDAVFISALKGTGLEDLLERIGSVLERAMVTLTLEVPYS
                     RGDLVAAVHRVGEVIEEKHDDNGMILEVRIPETARGQFAPFSR"
BASE COUNT          682 a         1285 c         1345 g          738 t
ORIGIN      
        1 cggccagctc gccggggaga tagtcgacgg gcctcgcaag gcggctttcc tcaggtcgat
       61 cgccgaggcc gagggcatcc ccctgtcaca ggtcgttgcc gtgggcgacg gagccaacga
      121 cctcgacatg ctggccacgg cagggctggg tatcgcgttc aacgccaagc cggtggtcaa
      181 ggagagggcg gacaccgcca tctcggtgcc ttatctggac gcgatcctgt tcacgatggg
      241 gatccgtcgc cagcacgtag aggaggccga cgaggccgat cccgggttcg aagtcgggga
      301 gcgtatcgag gttcccggta ccccacccgt ctgagtgccc tcaccagtcg tggtggaaga
      361 attcacccct ggggccatcc acacgttcaa aggtgtgcgc cccgaagaag tctcgctgcg
      421 cctggatcag gttggccggc agccgctccg atcgcatccc gtcgaagtag gcgagcgcgc
      481 tggagtaggc cggcgccggg atgcccgaga gggcggctcg cgccaccacg tcccgccatg
      541 aagtctccgc ttctaggagg gccttggaga agaaaccgtc ctccatcatg tcgggtaggc
      601 ccgggtctcg caggaaggcg gcggtgatgt cctcgaggaa cgccgcccgg atgatgcatc
      661 ccgcccgcca gatcgaggcg atcgtcgcaa gatcgaggtc ccagccgtat tcgtcagacg
      721 ccgctcgcat cagccggaat ccctgggcat aggagatgat cttcgaggcg tagagggcgc
      781 tctccacgtc gtcgggcgtc acctcatcca gaccgtgggg cgcattcgga tagatgacag
      841 cagctctcgt gcgcgcatcg gggtcggagc tgacgatccg ggcgtaaacc gcctcggcga
      901 ccagcgtcgt cggctggccc aggtccaacg acgagatcac tgtccatttg ccggtgcctt
      961 tttgcccggc ggcgtccagg atcacgtcga ccaccggctg gccctccagc tctctggtca
     1021 ggatttcgct ggtgatttcg atgagatagg agcggaggcg gccttcgtcc cactttgcga
     1081 agaccttgga catctccatc ggcgtcatgc ccatgccccg acgcatcagg tcgtaggcct
     1141 cggcgatgac ctgcatgtcc ccgtattcga tgccgttgtg gaccatcttg acgaaatggc
     1201 cggcgccacc tgttccgatc cactcacagc aaggcctgcc atccggagcc ttggcggcga
     1261 tcgatttgag catcggctcg acgtgctccc acgccttcct gctcccgccg ggcatgatcg
     1321 acgggccgtg acgcgccccc tcctcaccac ccgagacgcc catgccgaga taaaggaggc
     1381 cccgggactc gacgaatgcc tgacgacgct cggtgtcgct gtagagcgag ttcccgccgt
     1441 cgatgatgat gtcgccttcc tccatgtggt cgagaaggct gtcgatcacc gcgtccaccg
     1501 gtttgcctgc ggtcaccatg atcaggacca cgcgcggtcg gctcaacatc cccaccatct
     1561 cttcgatcga ggtggcgggg aggatgccca attccccggc ctctccggcc atgaactcgt
     1621 cagtgaccga cgccgtcctg ttgaaggcac cgacccgatg ccctttgtcg gcgagattca
     1681 gagcgaggtt tgctcccatc actccaagcc cggcaaccgc gatatcggcc tgaccgctca
     1741 tgcaaatcga gggtagctgg cctcgaattc gcacccacta gtctcgcccg ccgtgacaac
     1801 gaggatcggc gtcgacgtgg gtgggtccgg cgtgaaggcc ggagtggtcg acgtggaagc
     1861 aggtgagttg gtcagcgagc ggctccgggt ggacacaccg gagccgtcca cgcctccggc
     1921 ggtggctgct gccgtcacag agttggtgga ccaactcgac gcacctgggc gcatcggtct
     1981 cggctttccc tcggtgatgc gccagggatg ggtgactacc gccaacaaca tcgacaggtc
     2041 ctggatcggc gtcaacgctc tcgagctgtt cgagaccgcg gtcgggcggg agatcagctt
     2101 gctcaacgat gccgacgccg caggtctggc cgaagtccgg tacggggcgg ccaagggggt
     2161 gactgggaaa gtgctggtgc tgacctttgg caccggaatc ggcagcgccc tcatcgtcga
     2221 cggcaagctc ttgcccaaca tcgagctggg ccagctagag ctcggtgggg tgcgtccggc
     2281 ggagaagcgt tattcggcca agtcgagacg ggctgagaac ctcagctggg acgaatgggg
     2341 cgcgcgggcc cgtgagttca tcgttctggc caacgaggtc ttcactcctg atctcatcgt
     2401 gatcgggggc ggtgtctcca agttctggga tgagttcgcc tcacacgtca ccttcgatga
     2461 cgtcaagacc accaaggcgg cgctggccaa caacgccggg atcgtcgggg cggctgctct
     2521 cgccttcagc gggtgaaatt caacgactga acggtgcgaa ctggccgcgg gcggtctcgg
     2581 ggatcctcac ctcgaggatc attccgttgt cgtcgtgctt ctcctcgatc acctcgccca
     2641 cgcgatggac ggcggccacc agatcccccc gcgagtaggg gacttcgagc gtgagagtga
     2701 ccattgcccg ctccagcacc gaaccgatcc tctccaacag gtcctcgagc ccggttccct
     2761 tgagagcaga gatgaacacg gcgtccgggt agagattgcc caagcgcgtc agagcgtggt
     2821 ccgcgacccg gtcggccttg ttgatcgcga tcagctcggg gatctcggcc gccccgatct
     2881 cgaggagcac ctcgcgcacc gcggcgatct gcccggcggg atcagggtcg gtgccgtcga
     2941 ccatgtgaag aaggaggtcg gcctcgccca cctcggacag tgtggatcgg aaggcctcca
     3001 ccagatgatg gggcaggcgc cgaacgaacc caaccgtgtc ggcgaggagc actgtctgtt
     3061 ggtcgggcaa ctcgaggcgg cggaccgtcg aatcgagcgt ggcgaagagc cggtcctcga
     3121 cgaggacatc ggcatcggtg agggcattca gcagggtgga ctttccggcg ttggtgtacc
     3181 cgacgatcga tatctgggcg atatcggact tcctccgggc cttgcgctgg gtcgccctgt
     3241 gcttcaccgt ctctttcaac tctcgttcga gcttcgagtt gcgctccagg atgcgtcgac
     3301 ggtcggtctc gagcttggtc tcaccgggtc cgcgagccag aacgccggcg ccttgctgag
     3361 agaggctggt gccccggccg cgaaggcggg ggaggttgta ccggttcatc gccagctcca
     3421 cttggagcgc accgaccttg gtatgggcgt gctgggcgaa gatgtccagg atgagaccct
     3481 cccgatcgac gacgtcgcag ccgaacaact tctggagatt gcgctgctgg gccggggtga
     3541 gctggttgtc gaaaacgacg acatcgatgt ccagggcgtt ggtcaggttg gcgatctcct
     3601 ccgccttccc ggacccgatg taggttgccg ggtcgatccg ggtgcgtcgc accagctcct
     3661 gatcgaccgg ctccgatcct gcggtatcgg tgaggagagc aagctcctcg aggctgcgct
     3721 cggaatcgac agtggtcatt ccgggcagct cgacgccgac caggaaggct cgctggatgg
     3781 cgacttcgag gtcggtgatg gtcgcggtga ggcgacgacg gcgtctggag gcgtgttcct
     3841 gggtcatgag tctcgccgac cgagatgttt gcgggctcgc ccgcccgatg ccgggtgatt
     3901 taggccgctc agaggtactg gccctcgtct cccacgtgat cggcttcttc ctcggcgatg
     3961 atctgggact cgcgcaccgc gatcactggc ttaccggtgg aaggccaaat gaaccgccac
     4021 gcaccggtgg gcacggccca gaagcgttgg
//
LOCUS       DASTXO010000337         3379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1751547, whole genome shotgun sequence.
ACCESSION   DASTXO010000337 DASTXO010000000
VERSION     DASTXO010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1751547"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="VFU96_07135"
     CDS             complement(<1..985)
                     /locus_tag="VFU96_07135"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5113079.1"
                     /translation="MTPERVQRTYLLLLLLQTLAASLIWGINTLFLLDAGLSITQAFV
                     ANAGYTAGMVIFEVPTGVIADTFGRRISFMLGAGTLLVTTAAYLGLWYLEASIGWWIL
                     VSVLIGLGFTFFSGATEAWLVDALHATGFDGAIENVFGRGQIVGGAAMLVGTVGGGFL
                     GQINLGIPYALRSLLLLAVIGAAWLWMHDLGYEPTRGVAVGQQVRGILRASIKHGFGN
                     PPIRMFMFAAPFAAGVSIWAFYAFQPYLLELFGDPNATYLAGIAAAVFAVAQMAGGAS
                     VKYVRGLVETRTAVIILEVVLGAIALVGVGLAEMLQIPVGFWVAIGLLTLLS"
     gene            1025..2032
                     /gene="speB"
                     /locus_tag="VFU96_07140"
     CDS             1025..2032
                     /gene="speB"
                     /locus_tag="VFU96_07140"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEU5113080.1"
                     /translation="MYTPYEQRKGEIPFASSEYISFASRNSFLGVPAVTMDDLGEGSV
                     VILGAPFDWGTTYRPGARFGPQAIRNADYGAMDGYRPHLPTGLDPFETLGVVDVGDVY
                     VRPGELEISIGRIADAVEQVSAAGKVPIVLGGDHTITWPDALGVARVHGFGEIALIHF
                     DAHADTGFTQNGSLYGHGTPMRRLIESGAVPGHRFVQIGLRGYWPEPEVMQWMREQKM
                     RSFLMNEIVDRGLNDVVDEAVAYSLEGGAKGVFISVDIDVVDPGLAPGTGTPEPGGLG
                     SRELLDTVRRLSRDLNVLGADIVEVSPPYDGPGEQTSYLANRVVLEILNGMAERKTAT
                     S"
     gene            complement(2022..2570)
                     /locus_tag="VFU96_07145"
     CDS             complement(2022..2570)
                     /locus_tag="VFU96_07145"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEU5113081.1"
                     /translation="MTQGPPTGPSTGDDLQPLPPPESPAEDPVPEPSMGQLAASGQAG
                     PPSAPIEPRPQARPPARTGDQQMWQVLAHASAFVQVIGIPSLVGPLVVWLMKRDDPAV
                     EPHARGALNFQLSLLIYFIVGGIIGVIFAITVIGLVVAIPIFLFLLVLVVLELVFALM
                     ASLAASRGELYDYPMSIELIKK"
     gene            complement(2567..3208)
                     /locus_tag="VFU96_07150"
     CDS             complement(2567..3208)
                     /locus_tag="VFU96_07150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5113082.1"
                     /translation="MSEVTIINLQAEMAADLERIELTCFPMANPDDLLSKEDIVAYAE
                     VFPEGYFVAIADGRPVGMGAGIYLDFDFDHPQHDIAGITGEHQCANHDPDGDWYYGTD
                     ITVLPEYRGRGIGGMLYEKRKGLVIRDGKRGIVAGGSLPGYFQHKSTMPIDEYVSRVV
                     AGELHDPTLSFQLSHGFEVRGLLENYLEDESDDGWAALIVWENTSSPPRTIDR"
BASE COUNT          654 a         1079 c         1080 g          566 t
ORIGIN      
        1 cggaaagcag tgtgagcagc ccgatcgcca cccagaatcc gaccgggatc tgaagcatct
       61 cggccagacc cacacccacc aacgcgattg cgccgagaac gacctcgagg atgatgaccg
      121 cggtccgggt ttcgaccaaa cctcgcacat atttcaccga ggcgccgccg gccatctggg
      181 cgacggcgaa caccgcggct gcgatcccgg ccagataggt ggcattgggg tcgccgaaca
      241 actcgagcag atagggctgg aaggcgtaga aagcccagat gctcaccccg gccgcgaagg
      301 gcgcggcgaa catgaacatc cggatcggag gattgccgaa tccgtgcttg atcgatgccc
      361 tcaggatccc gcgaacctgc tgtccaaccg ccacgcctcg ggtgggctca tacccgagat
      421 cgtgcatcca gagccacgcc gctccgatca ccgcgagtag gagcagcgag cggagggcat
      481 agggaattcc cagattgatc tgtccgagga agccacctcc aaccgtgccc accagcatgg
      541 ctgccccgcc cacgatctgg ccccggccaa agacgttctc gatcgcccca tcgaagccag
      601 tggcgtggag agcgtccacc aaccatgcct cggtggcgcc ggagaagaag gtgaacccga
      661 gaccgatcaa caccgagacc aggatccacc agccgatcga ggcctcgaga taccagagcc
      721 cgagataagc ggccgtggtc accaggagag ttcccgcacc gagcatgaat gagatccgcc
      781 tcccaaaggt gtcggcgatc acccccgtgg gcacctcgaa gatgaccatg cccgcggtgt
      841 accccgcgtt ggctacgaac gcctgcgtga tgctgagccc cgcatcgagc aggaacaacg
      901 tgttgatccc ccagatcagg gatgctgcga gcgtttgaag aaggaggagc aggagatagg
      961 tgcgttgaac gcgttctggc gtcatggctc accgaggggg tctggcagtt agcgtaacga
     1021 tccgatgtac accccctacg aacaacgaaa gggcgagatt cctttcgcct cctcggaata
     1081 catctctttc gcctcacgca attccttcct cggtgtcccc gccgtcacca tggacgatct
     1141 cggagagggt tcggtggtca ttctcggcgc accgttcgac tggggaacca cctaccgccc
     1201 gggggctcgg ttcggccccc aagcgattcg aaacgccgac tacggggcca tggacggcta
     1261 ccggccccac ctccccaccg ggctcgaccc attcgagaca ttgggggtgg tggacgtagg
     1321 cgatgtctac gttcggccgg gcgagctcga gatctccatc gggaggatcg ccgacgccgt
     1381 cgaacaggtg tctgccgccg gcaaggtgcc catcgtcctc ggtggtgacc acaccatcac
     1441 ctggcccgac gccctcggcg tggcccgggt gcatgggttc ggcgagatcg ccctgattca
     1501 tttcgacgcc cacgccgaca ccgggttcac ccagaacggc tccctctacg ggcacggcac
     1561 gccgatgcgg cggctgatcg agtcgggagc ggtgcccggc caccgtttcg tccagatcgg
     1621 gttgcgcggc tactggcctg agccggaggt gatgcaatgg atgcgggagc agaagatgcg
     1681 ctcgttcctg atgaacgaaa tcgtcgaccg gggtctcaac gacgtggtcg acgaggcggt
     1741 ggcctattcg ctcgagggtg gcgcaaaggg tgtcttcatc agtgtcgata tcgacgtggt
     1801 cgaccctggc ctggccccag ggaccggaac ccccgagccc ggtgggctcg ggtcacgtga
     1861 gcttctcgac acggtacgga ggctctcccg cgatctgaat gtgctcggag cggacatcgt
     1921 cgaggtctcg ccgccctatg acgggcccgg tgagcagacc tcctatctgg ccaatcgggt
     1981 cgtgctcgag atcctgaacg ggatggcaga gcgcaagacc gctacttctt gatcagctcg
     2041 atgctcatcg gatagtcgta cagctctccc cgtgacgccg ccaacgacgc catgagggca
     2101 aagaccaatt cgaggaccac cagcaccaag aggaacagga agatcgggat cgccaccacc
     2161 agcccgatca ccgtgatcgc aaagatgacc ccgatgattc ccccaacgat gaaatagatc
     2221 aggagcgaga gttggaaatt gagggcgccg cgggcatggg gctccaccgc tggatcgtca
     2281 cgcttcatca gccagaccac caacggaccg acgagtgagg ggatcccgat gacctgaacg
     2341 aaggccgacg cgtgggcgag cacctgccac atctgctggt caccggtcct cgccggtggt
     2401 cgagcctgtg gtcgtggctc gatcggtgcg gacggcggcc ctgcctgccc ggaggcagcg
     2461 agctgtccca tcgaaggctc gggtaccggg tcttctgccg gggactcagg cggcgggagc
     2521 ggctggagat cgtcgccggt gctaggcccc gtgggcggtc cttgggtcat cggtcaatcg
     2581 tacgcggcgg cgaggaggtg ttctcccaga cgatcaaggc ggcccagcca tcgtcggact
     2641 cgtcctcgag atagttctcg agcaaaccgc ggacctcgaa tccatgcgag agctgaaagc
     2701 tgagcgttgg gtcgtgaagc tccccggcga cgacacggct cacgtactcg tcgatcggca
     2761 tcgtcgactt gtgctggaag tagccgggca atgagccgcc ggcgacgatg ccccgctttc
     2821 catctcggat cacgaggcct ttccgcttct cgtacaacat gccgccgatg ccccgacccc
     2881 ggtattcggg gaggacagtg atgtcggtcc cgtagtacca gtcaccgtca gggtcgtgat
     2941 tggcacactg gtgttcgccg gttatgccgg caatgtcgtg ctgggggtga tcgaagtcga
     3001 agtcgagata gatgccggcg cccatcccga cggggcggcc atctgcgatt gccacgaagt
     3061 agccctccgg gaacacctcg gcgtaggcga cgatgtcctc tttcgacagc aggtcgtcgg
     3121 ggttggccat cggaaagcac gtgagctcga tgcgctccag gtcagcggcc atttccgcct
     3181 ggaggttgat gatcgtcacc tcgctcacag acggaaggac tcccatgggc tgtcccccgg
     3241 gataccccag accgattcga gcttcttctc cctggggcgg atgaccaccg caccacacgt
     3301 ggccacgtcg ccgggcgatt gggctctgcg tgctatcgac ggatcggaga agaagtggtc
     3361 gaggtcctgg gagagctct
//
LOCUS       DASTXO010000338         2198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_204589, whole genome shotgun sequence.
ACCESSION   DASTXO010000338 DASTXO010000000
VERSION     DASTXO010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204589"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VFU96_07155"
     CDS             <1..410
                     /locus_tag="VFU96_07155"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU5113083.1"
                     /translation="IEIDAQQERVWGVLTDLEAWPRRIETVEVVELLTPAPVGDGSRI
                     RLKQPKLGEGTWDITVWDAPSYFEMRQKSGGITTDAGHRVESIGEGRSRLTLTLDMRG
                     PLTVIARFFKGLTNRYMSIEAQGMRVAAESAET"
     gene            complement(535..621)
                     /locus_tag="VFU96_07160"
     tRNA            complement(535..621)
                     /locus_tag="VFU96_07160"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(585..587),aa:Leu,seq:gag)
     gene            complement(646..888)
                     /gene="secG"
                     /locus_tag="VFU96_07165"
     CDS             complement(646..888)
                     /gene="secG"
                     /locus_tag="VFU96_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU5113084.1"
                     /translation="MAVVEAIAIVIHIILSLAVVALVLLHSGKGGGLSDVFGGGMSTS
                     SLGGSTVVERNLDRLTVIVGILFGISTFGLTFLLNS"
     gene            complement(964..1707)
                     /gene="gpmA"
                     /locus_tag="VFU96_07170"
     CDS             complement(964..1707)
                     /gene="gpmA"
                     /locus_tag="VFU96_07170"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEU5113085.1"
                     /translation="MTYRLVLLRHGQSTWNLENLFTGWTDVGLTEQGEEEAREAGRLI
                     VSEGLDPGILHTSVLVRAIKTAELTLEEMGRQYLPVRRSWRLNERHYGALQGLNKKET
                     ADLHGAEQVLLWRRSYDIPPPALDLADERHPRHDLRYDDLAPDLLPATECLKDVVVRM
                     LPYWHDTIVPDLLAGREPLVVAHGNSIRALVKHLDGISDEEIVDLNIPTGVPLLYELD
                     DSFSPVSSRYLGDPEAAEAAAAEVARQAG"
     gene            complement(1704..>2198)
                     /locus_tag="VFU96_07175"
     CDS             complement(1704..>2198)
                     /locus_tag="VFU96_07175"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU5113086.1"
                     /translation="VDHVIVGHSERRQGFGETDEMVAKKAKAVAQAGMHPIVCVGETL
                     EQREAGEAESLVVSQVIASLARLDADALATSAIAYEPIWAIGTGRTALPDDAGTMASI
                     IRETVADKVSSDTAESVRILYGGSVNAANIKDFMAKADVDGALVGGASLDPDSFAAIV
                     RYWV"
BASE COUNT          414 a          699 c          714 g          371 t
ORIGIN      
        1 cgatcgaaat cgacgcgcag caagagcgcg tatggggggt cctcaccgac ctagaagcgt
       61 ggccgcgacg tatcgagacc gtcgaggtcg tcgagctact gacgccggcg ccagtgggcg
      121 acggtagccg gatacgcctc aagcaaccga agctgggaga gggcacctgg gacatcacgg
      181 tctgggacgc gccgtcctac ttcgagatgc gccagaagtc gggcggaatc accactgatg
      241 cgggccatcg cgtcgagtcg atcggggaag gccgctcgcg cctgacgctg accttggaca
      301 tgcgaggtcc gctgacggtc atcgcccggt tcttcaaggg cctgaccaac cgctacatgt
      361 caatcgaggc ccagggcatg agggtcgctg ccgaatccgc cgagacgtga ggtaacaccc
      421 cgcctagggc gtgtcggaga gaggactcga acctccacgg ggagtaatac gccaccggcc
      481 ctcgacctga agcgtccatc cagcaccgtc gccactcggt ggcggtcgac gaagtgtcgg
      541 agagaggact cgaacctcca cggggagtaa taccccacca ggccctcaac ctggcgcgtc
      601 taccaattcc gccactccga ctgaagcggg gcgagtctac gcccgtcagc tgttcaagag
      661 gaaggtcagc ccgaaggtgc tgatgccgaa aaggatcccg acgatcacgg tgagccggtc
      721 gagattcctc tcgacgacgg tgctaccacc gaggctggag gtcgacatcc cgccgccaaa
      781 cacgtccgag agaccgccac ctttgcccga gtggagaagg acgagcgcca ccacagccag
      841 cgagagaatg atgtggatga cgattgcgat tgcttcgacg acagccattg cctgcctgtt
      901 gagatcgagg gatgacacgg tttcccgtgc gtcggggatt ctaccagcga ctccccgctt
      961 cacctacccg gcctggcggg caacctccgc tgccgcggcc tcggcggcct ccggatcgcc
     1021 gaggtagcgg ctggacactg gcgagaaaga gtcatccagc tcgtagagca gcgggacgcc
     1081 cgttgggatg ttgagatcga cgatctcctc atcgctgatc ccatcgagat gcttgaccag
     1141 ggcccgtatc gaattgccgt gagcgacgac cagtggctcc cgaccagcga ggagatcggg
     1201 aacgatggtg tcgtgccagt agggaagcat ccgcaccacg acgtctttga ggcactccgt
     1261 ggcgggcagg agatcaggag ccaggtcgtc gtagcgcagg tcgtgacgtg ggtgacgctc
     1321 gtcagcgagg tcgagcgcgg gaggtgggat gtcgtaactg cgacgccaca gcagcacctg
     1381 ctcggcgccg tgcaggtcgg cagtctcctt cttgtttagg ccttgcaggg cgccgtaatg
     1441 acgctcgttg agacgccagg accgacggac cggcagatac tgcctcccca tctcctcgag
     1501 agtcagctcc gcggtcttga tcgcccgcac caggaccgac gtatgcagga tgccgggatc
     1561 gagcccctcg ctcacgatga gccgccccgc ctcacgagct tcctcttcgc cctgctcggt
     1621 caggcccacg tcggtccagc cggtgaacag gttctccaga ttccatgtcg actggccgtg
     1681 gcgaaggagt accaggcgat aggtcatacc caataccgga cgatcgcggc gaagctgtcg
     1741 gggtcgagtg atgcgccgcc gacgagggca ccgtcgacgt ccgccttggc catgaagtct
     1801 ttgatattcg cggcgttcac cgacccgcca tacagaattc gaacactttc ggcggtgtcc
     1861 gaggagacct tgtccgcgac ggtttccctg atgatgcttg ccatggtgcc ggcgtcgtcg
     1921 ggcagagcgg tgcggccggt gccgatagcc catatcggct cgtaggcgat tgccgacgtg
     1981 gcgagggcgt ccgcgtcgag gcgagccagg gaggcgatca cctgggatac gacaagactc
     2041 tcggcttcgc cggcctcacg ctgttccaag gtctcgccga cgcagacgat ggggtgcatg
     2101 ccggcctgtg ccaccgcttt tgccttcttg gccaccatct cgtcggtctc gccgaagccc
     2161 tgacgacgct ccgagtggcc aacgatcacg tgatcgac
//
LOCUS       DASTXO010000339         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_20783, whole genome shotgun sequence.
ACCESSION   DASTXO010000339 DASTXO010000000
VERSION     DASTXO010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20783"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VFU96_07180"
     CDS             <1..597
                     /locus_tag="VFU96_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902789.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU5113087.1"
                     /translation="VIPGGFGYRGVEGKVQAIRYARENRVPLLGLCLGLQCSVIEFGR
                     NVLGLTEANSTEFDPTTPHPLIDLMASQEDVEEKGGTMRLGLYAAKLAEGSKTREMYG
                     QELVYERHRHRWEVNNRYRKDLEKAGMRLSGVSPDDNLVEYIELVDHPFFVGTQAHPE
                     FRSRPDNPHPLFVGLVRAAAARGDQVVELPEPVDLTVE"
     gene            662..1783
                     /gene="ald"
                     /locus_tag="VFU96_07185"
     CDS             662..1783
                     /gene="ald"
                     /locus_tag="VFU96_07185"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEU5113088.1"
                     /translation="MIVGVPKEIKTEEHRVAITPVGTRELTERGHTVLIEKGAGEGSS
                     IVDDEFEAQGATIVPNAGEVFGGAEMILKVKEPQDSEIELFRPGQILFTYLHLAAYPR
                     MAAALLGRDAIAIAYETVQLPDRSLPLLAPMSEIAGRMATQVGAYFLGKAEGGRGVLL
                     GGVTGVRSGKVVVIGGGIAGSNAAVIAVGMQADVVVLDTDINKLRALDSLYLGRVRTL
                     HSNRLTLEEEVLDADVVIGTVLVPGASAPKLVTEDMVRAMKRGAVLVDVAIDQGGCFA
                     TSRETTHTDPVYLIHDVVHYAVGNIPGAVPNTSTYALTNATLRYAVSLANEGVSGAVD
                     KHPELVGGINVVGESVCHPAVAESLGVAHVPPREALASV"
     gene            1780..>2128
                     /locus_tag="VFU96_07190"
     CDS             1780..>2128
                     /locus_tag="VFU96_07190"
                     /inference="COORDINATES: protein motif:HMM:NF014898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU5113089.1"
                     /translation="MSAGALTLPEGVDEYLLALDVERGLSTNTIEAYRRDLAQYLDFL
                     EGSEPDRSAVERYVAHLRGKELASSTVGRKLAAIRGLHRFLVIEGLRQADPTLLLESP
                     RRPEPFPKALTIDE"
BASE COUNT          389 a          676 c          698 g          365 t
ORIGIN      
        1 gtgatccccg gcggctttgg atatcggggc gtcgaaggca aggtgcaggc gatccgctac
       61 gcacgtgaga accgggtgcc gttgctcggt ctctgtctcg ggctgcagtg ctcggtgatc
      121 gagttcgggc gcaacgtcct cggtctgacc gaggccaaca gcaccgagtt cgatccgacc
      181 acaccacatc cgctgatcga cctgatggcg agtcaggagg atgtcgagga gaagggcggg
      241 accatgcgtc tcggtctcta cgcggccaag ctggctgaag gctcgaagac cagggagatg
      301 tatggccagg agctcgtgta cgagagacat cggcaccggt gggaggtcaa caatcgctac
      361 cggaaagacc tggagaaggc gggaatgaga ctctccggtg tctcacccga tgacaatctc
      421 gtcgaataca tagagctcgt ggaccaccca ttcttcgtgg gcacccaggc tcaccccgag
      481 ttccgttccc gtcccgacaa cccccacccc ctcttcgtgg ggctggtccg cgccgccgcg
      541 gccaggggag atcaggtggt cgagctgccg gagccggttg atctgaccgt cgagtgaggc
      601 acggccggcc ctgcgccgat agcctgccgt caaagctgcc aagcgtcagg gaggacgttt
      661 cttgatcgtc ggtgtcccca aggaaatcaa gaccgaagag caccgcgttg ccatcacgcc
      721 agttggcacg cgcgagctca ccgagcgtgg tcataccgtt ctcatcgaga agggagcggg
      781 cgagggatca tcgatcgtcg acgacgagtt cgaagcgcaa ggggccacca tcgtgcccaa
      841 cgccggcgag gtgttcggcg gggcagagat gatcctcaag gtcaaggagc ctcaggactc
      901 cgagatcgag ctgttccggc cgggccagat cttgttcacc tatctccatc tcgcggcata
      961 tccgcgtatg gctgcggccc tgctgggacg ggacgccatc gccatcgcat atgagacggt
     1021 gcaactgccg gaccgatcgc tgccactcct cgctccaatg tccgagatcg cgggtcgcat
     1081 ggccacccag gtcggtgcct acttcctcgg gaaggcagaa ggcgggcggg gagtgctcct
     1141 cgggggtgtc accggcgtcc ggtcgggcaa ggtcgtggta attggaggcg ggatcgctgg
     1201 atccaacgcc gccgtcatcg cagtcgggat gcaggcggac gtggtggtgc tcgacaccga
     1261 catcaacaag ctgcgcgccc tggactcgct gtatctcggc cgggtgagga cgcttcattc
     1321 caaccggctg acactggagg aggaggtgct cgacgccgac gtggtgatcg gcacagtcct
     1381 ggttccaggt gcgtctgccc ccaagctggt caccgaggac atggtccggg cgatgaagcg
     1441 tggggcggtt ctcgtcgacg tcgccataga tcagggcggc tgcttcgcca cgtccagaga
     1501 gacgacacac acggatccgg tgtacctgat tcacgacgtg gtccactacg cggttgggaa
     1561 catcccgggc gcggttccca acacgtcgac gtacgcactc accaatgcaa cgcttcgcta
     1621 cgcagtcagc ctggccaatg aaggcgtatc cggtgccgtc gacaagcatc cggagctggt
     1681 gggaggcatc aacgtcgtcg gggagtcggt ctgccacccg gcggttgccg agagcctcgg
     1741 cgtcgcacac gtgccgccac gagaggcact cgcctcggtt tgagcgccgg cgcgctgaca
     1801 ttgccggagg gcgtcgacga atacctcctg gccctcgacg tcgaacgcgg cctctcgacc
     1861 aatacgatcg aggcctacag gcgggacctc gcccagtatc tcgacttcct cgaggggtcc
     1921 gagccagacc ggtcggcagt ggagcgctat gtagcccacc tccgaggcaa agagctggcc
     1981 tcgtcgactg tcggtagaaa gctcgccgcg atccgcggtc tgcacaggtt cctggtgatc
     2041 gaggggctcc gccaggccga ccccactttg ctcctcgaat ctccccgtcg ccctgagccc
     2101 ttccccaagg ccctcaccat cgacgaag
//
LOCUS       DASTXO010000340         4207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_388937, whole genome shotgun sequence.
ACCESSION   DASTXO010000340 DASTXO010000000
VERSION     DASTXO010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388937"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..803)
                     /locus_tag="VFU96_07195"
     CDS             complement(189..803)
                     /locus_tag="VFU96_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113090.1"
                     /translation="MTLEVLEPPSVNRLYFWAVQASFLDGAGSHGAGHLGLQWNPRFP
                     DHKAVNWGGYATRGVILPGSGSPLPSTPQDANTRDFPWLPKRKYSLRIYPSPDMGWRG
                     EVTDLDTGVPTVVRDLHAGGDHLGRVVVWSELFCQCSDPRSVVAWSEPVAIGLSGDQL
                     SPRALRVNYQVDGCPNTNVYSDGHRVYQATASERLTPQGSLVMV"
     gene            complement(954..1385)
                     /locus_tag="VFU96_07200"
     CDS             complement(954..1385)
                     /locus_tag="VFU96_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HEU5113091.1"
                     /translation="MTDASLYDRLGGVFAIAAVVDRFSDALIENPIVGQESSNPALRE
                     WHTNNLGRLPGLKFMRTLWVCNVSGGPFEYSPTEPGSTTLGLEEAHREFRITSAEFDE
                     VAAELARTLDAFEIPAREKDEVLAAFAAHKDEVTEGSMATA"
     gene            1510..2904
                     /locus_tag="VFU96_07205"
     CDS             1510..2904
                     /locus_tag="VFU96_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014441930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU5113092.1"
                     /translation="MAEPERDQLSSPTADEAALQQFLLYLGSALTAAGEAVNEVQDHL
                     HAVAAAYGANRARFAVLPTFVVVALDPSRPATLEPTPQLRGVLRLDQTSALFEILKRA
                     TRAEIDPAEGSRQVLEVIARPSKYRTLSTLAGHVVLTVGICLILQPTWADVALAALFG
                     LVVGLLRLIGSRWVPAQTLMPIAAAFIVSALTFLLANQGWTNANLRTMIPPLVTFLPG
                     AALTMAVIELSAAQMVAGASRLVSGGLQLVLLAFGIVSAAQAFGLPSVEILVDDPQNL
                     IGWWAPWLGMVVFGIGVAVFHSAPSGSLPGLFLVLLVAFVGQQVGSALFGGYVGGFIG
                     AFAMTPTARLVERTRFGPPALVSFLPGFWLLVPGALGLIGVTEYIASGPSLGVQDFLG
                     TVGAMVAIAVGVLCGYLLVDAVKPLYTHTVRPALDLLPFQLVPPSWDAAFGEHEADRG
                     KRKETRPDPDEEDA"
     gene            2917..>4207
                     /locus_tag="VFU96_07210"
     CDS             2917..>4207
                     /locus_tag="VFU96_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020513885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5113093.1"
                     /translation="MTHPDPAPDVRSTDVKAGLGVLLATCISALVVNANTSAVSILLP
                     SIGEDVGAPVSTLQWAVTGYSLVGAAFIVTAGALGDVFGRRRIFLGGLALFFVSCALI
                     ALSDTAAGVIGGRAIQGAAGSTILACGLSLLTVASSGAERLRAVSYWGAASAIGAAAG
                     PLVGGVLVDTAGWQALFWIDAGIAALCVPLTLRRVEESLDPNRSRSIDWLGTFLVAAI
                     LAPFILAVSKGSAWGWTSPATLGSFLVSVVAAIAFVYVERRSEAPLVDLDLMRNRALI
                     GATLGILIGAGTINGLMFVFSLYFQDPSTLGMSALEAGLATLPATVGLVILAPLVPRF
                     AIKMGTRQVVGLGFIIMTAGFAVLIGTSSSWAYAAFVLPLIAVAAGMALSNGPCSSVA
                     TSAVPEEQVGSASGISNMARYVGAAVWTAVVATLYADV"
BASE COUNT          635 a         1377 c         1373 g          822 t
ORIGIN      
        1 gtgctcgaca cctgcggtat tgatattcgg ctcgccaagt tggacagccg ggcgggtgag
       61 gtgatcgaca cgttccattt cggcacaggt ccggcgacag ccggtctcga cctgcgcgga
      121 ttggagcaac ggatcgccga cggcgtcacg tcctgatctg acgactcccg atcaccggaa
      181 gggggcggtc agaccatcac cagagaccct tggggcgtga gccgctcgct ggcggtggcc
      241 tggtacaccc gatgaccatc ggagtagaca ttggtgttcg ggcaaccatc gacctggtag
      301 ttgaccctga gggctcttgg gctcagctgg tctccgctca ggccgattgc gaccggctcg
      361 gaccaagcca cgacgcttcg gggatctgag cattgacaga agagctcgga ccagacgacc
      421 acccggccga gatggtcgcc accggcgtgg aggtcgcgga cgacggtcgg cacgccagtg
      481 tcgagatcgg tgacctcgcc cctccagccc atgtccgggg aggggtagat ccgcaggctg
      541 tattttcgct ttggcagcca gggaaaatcg cgcgtattcg catcctgtgg agtgctgggg
      601 aggggtgagc cagaacccgg gagtatgacg cctcgagtgg catagccccc ccagttgaca
      661 gccttgtggt cggggaatct ggggttccac tgcagcccca gatgtcctgc tccgtgcgat
      721 ccggcgccgt ccaagaagga ggcctgcacg gcccagaagt agagacgatt caccgacggc
      781 ggctccagga cctcgagcgt caccgccacc tcacgagcgg gtggagcgag atcccatgcg
      841 agatggaacg agaaggccat gccggttttc gggcgacgtg agccgccgcg gctcagcgta
      901 ccggcgaccg cgcccggcgc cgggctgaat caatccacgg cgttggacgc gccctaggcc
      961 gtggccatgg agccctcggt cacctcgtcc ttgtgtgcgg cgaacgcggc gaggacctcg
     1021 tccttctccc gcgccggtat ctcgaaggcg tcgagcgtcc gagccagttc ggcagccact
     1081 tcgtcgaact cggcagatgt gatccggaat tcgcgatgag cctcctcgag ccccagcgtt
     1141 gtgctccccg gctcggtcgg gctgtactcg aacggtccgc ccgacacgtt gcaaacccac
     1201 agagttcgca tgaacttgag accgggaagc ctgcccaggt tgttcgtgtg ccactcgcgc
     1261 agagccgggt tgctcgactc ctggcccacg atgggattct cgatcagggc atcactgaat
     1321 cggtcgacca ctgcggcgat ggcgaatacg ccacccaacc tgtcgtacaa actcgcgtcc
     1381 gtcatgactc gtcctcctgc tcgcttaccc agggacccct ctattagcgg tcaatctggt
     1441 ttttacaaca gaacgctgga ggagtcaacg gtgcggtcag ccagcccctc gtcgtggcat
     1501 gattttcctg tggccgagcc agagcgagac caactgtcct ctcccactgc agacgaggca
     1561 gcgctgcagc agttcctcct ctacttgggc agtgccctga ccgccgccgg cgaagccgtg
     1621 aacgaagtcc aggatcacct ccatgcagtc gccgcggcct acggcgcaaa cagagctcgc
     1681 ttcgcggtgc tgccgacgtt tgtggtggtc gcactcgatc cgagtcggcc ggcaaccctg
     1741 gagcccactc cccagctcag aggggtgctc cgactcgacc agacgtccgc cctcttcgaa
     1801 atcctgaaac gcgccacccg ggccgagatc gatccggccg aaggaagccg tcaggtgctc
     1861 gaggtgatcg cccgcccctc gaaataccgg acactgtcga cactggccgg gcatgtggtg
     1921 ctcacggtgg ggatatgcct gatcctccag ccgacctggg ccgacgtggc gctggccgct
     1981 ctcttcgggc tggtggtcgg cctcctcaga ctcatcggat ctcgctgggt gccggcgcaa
     2041 accttgatgc ccatcgctgc ggcgttcatc gtgtcggcgc tcaccttcct gctcgcaaat
     2101 cagggatgga ccaacgccaa cctgaggacg atgatccccc ccctcgtaac ctttctcccc
     2161 ggagccgccc tcaccatggc ggtgatcgaa ttgtcggcgg cacagatggt ggccggcgcc
     2221 agtcgcctcg tctccggtgg gttgcaactc gtcttgctcg cgttcgggat cgtgagcgcg
     2281 gcccaggcct ttggcctgcc ctcggtagag atcctcgtgg acgatccaca gaatctgatc
     2341 ggctggtggg cgccatggct gggcatggtc gtgttcggga tcggagtcgc cgtcttccat
     2401 tcggcgccgt cgggttcgct gcccgggctt ttcctggtcc ttctggtggc ctttgtgggt
     2461 cagcaggtgg gcagcgcact cttcggcggc tacgtcggtg gcttcatcgg agccttcgcc
     2521 atgacaccca ccgcgcgtct cgtagagcga accagattcg gccctccggc tctggtctcg
     2581 ttccttcccg ggttctggct gctggtccca ggtgctctcg gcctgatcgg ggtgacggag
     2641 tacatcgcgt ccggtcccag cctcggggtg caggacttct tggggacggt tggcgcgatg
     2701 gtcgcgatcg cggtgggggt cctctgcggg tacttgctgg tggatgcggt caagcccttg
     2761 tacacacaca cggtgcgtcc ggccctcgat ctcctcccgt tccagttggt gccaccgagc
     2821 tgggatgcgg cattcgggga acatgaggcc gatcgaggga agcgcaagga aaccaggccc
     2881 gatcctgatg aggaagatgc ataggatcgg ggacccatga cccaccctga cccggcgccc
     2941 gacgttcgca gcactgacgt caaagcaggt ctcggtgttc tcctcgccac ctgcatctcg
     3001 gcattggtgg tgaatgccaa cacctcagcg gtgtcgatcc tgctcccctc gattggagag
     3061 gatgtcgggg cgccggtgag caccctgcag tgggcggtca ccggctattc cctggtggga
     3121 gcagcgttca tcgtcaccgc cggcgccctc ggtgacgtct ttggccgtcg gcgcatattt
     3181 ctcggcggcc tcgccctgtt cttcgtctcc tgtgcgctca tcgccctgag cgacaccgcg
     3241 gccggggtca tcggcggccg tgccatccag ggggcggccg gctcgacgat cctggcgtgt
     3301 ggcctgagcc tcctcaccgt cgcttcctcc ggagcggagc gtctcagggc cgtctcatat
     3361 tggggagcgg cgtcggcgat cggcgccgcc gccggccccc tggtcggtgg ggtcctcgtc
     3421 gatacggcgg gatggcaggc gttgttctgg atcgacgccg ggattgccgc cctatgcgtg
     3481 ccccttactc tgcggcgggt cgaggagtcg ttggacccga accgatcgcg cagcatcgat
     3541 tggctgggca ccttcctcgt cgcggcgatc ctggccccgt tcatcctcgc ggtgagcaag
     3601 gggtccgcat ggggttggac gtcaccggcg acactcggct cgttcttggt ctcggtggtg
     3661 gcggcgatcg ccttcgtgta tgtggaacgc cgatcggagg cgccattggt cgatctcgac
     3721 ctgatgcgca accgcgctct gattggggcc acgctgggaa tcctgatcgg tgcgggcacg
     3781 atcaacgggc tcatgttcgt gttcagtctc tacttccaag acccgtcgac gctcggcatg
     3841 agcgctctcg aggcgggcct cgccacactc ccggccacag ttggtctcgt gatcctggcc
     3901 ccactggttc ccaggtttgc gatcaagatg ggcacccggc aggtagtggg tctgggtttc
     3961 atcatcatga ctgcagggtt cgccgtgctc atcgggacgt cgtcgtcgtg ggcatacgcc
     4021 gccttcgtcc tccccctgat cgccgtcgcc gccggaatgg ccctttccaa tgggccatgc
     4081 tcgtcggtcg ccacgagcgc ggtccccgag gagcaggtcg ggtccgcctc cgggatatcc
     4141 aacatggccc ggtacgttgg ggctgcggtc tggaccgcgg tggttgccac gctgtacgcc
     4201 gatgtca
//
LOCUS       DASTXO010000341         2070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1052097, whole genome shotgun sequence.
ACCESSION   DASTXO010000341 DASTXO010000000
VERSION     DASTXO010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052097"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..571)
                     /locus_tag="VFU96_07215"
     CDS             complement(125..571)
                     /locus_tag="VFU96_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011981165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEU5113094.1"
                     /translation="MGRKLQAGNKVFLPRVGAVMDTTVREALDRGGTVDITTIGRKSG
                     LPRRTEIYFHQFDGKFYLTGRPGFKRDWVANIVANPEFTLHLKHGVTADIPVRGEPEP
                     DPEERARILFRALTESWGSDPERSRAALDRWVETAPFIRFAPVDQT"
     gene            572..1792
                     /locus_tag="VFU96_07220"
     CDS             572..1792
                     /locus_tag="VFU96_07220"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEU5113095.1"
                     /translation="MGQLIQVEASPLGAVTLFSTDRSLTGQDGVSLAPGASDGADPPH
                     ELARRLFDADDLIDHVHVLSNTVAVRRRESWDEEAVDRARDVIANLFVHYGTGPAPSG
                     ADEFEQLRVQNYNATLSHIRAHNEDLWVMRVTPDQPIDPFLPGQYTTLALGYWEPRAD
                     DAHDNLKPDQDQKMARRSYSVSSSMIDETGELLPPHTPDVEFYIVKVKPGQEEIPALT
                     PRLFMKDEGDRLYMGRKFTGHYTLEGVSPDDNIVFLSTGTGEAPQNAMIAELLRKGHE
                     GHMLNVVCVRYRSDLAYTEQHAVLVDRYSNYKYVTITTREPENEGKKVYIQDLVTSSQ
                     IEKELGAPMDPDHTHVFLCGNPAMIGLPKWTDDGMVFPETLGVCQQLHEKGFTIDHRK
                     ERGNVHYEEYWTDR"
     gene            1875..>2070
                     /locus_tag="VFU96_07225"
     CDS             1875..>2070
                     /locus_tag="VFU96_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018530605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113096.1"
                     /translation="MSTTKKTDGFSTAERAAMKARAQELKAQGRSGQKQADNEQAVLD
                     AIAEMPDADRTIAERIHALVT"
BASE COUNT          413 a          671 c          637 g          349 t
ORIGIN      
        1 catcgctctt cacctcggtg gttgccttcg gcgtggccgc catggctgtc gcagtcggcg
       61 ttctcttcgt cctgatcgga ctcggcatcc gggacgtggc ccagcgaacc accgagccgt
      121 cgactcaggt ctgatcgact ggcgcgaagc gaatgaaggg agcggtttcg acccaccggt
      181 cgagcgccgc tctcgaccgc tccgggtctg agccccagct ctcggtcagc gctcggaaga
      241 ggatccgggc gcgttcctcg ggatcgggct caggctcacc gcgaaccggg atgtcggcgg
      301 tgaccccgtg cttgagatgc agggtgaact ccggattggc cacgatgttg gcgacccagt
      361 cacgcttgaa cccgggccgg ccggtgaggt agaacttccc gtcgaactga tggaaataga
      421 tctcggttct ccgaggcaga cccgacttcc gaccgatcgt cgtgatgtcc accgtgccac
      481 cgcggtcgag tgcttccctg acggtggtgt ccatgaccgc accaaccctg gggaggaaca
      541 ccttgttccc ggcctgtaac ttgcgtccca catggggcaa ttgatccagg tcgaggccag
      601 tccgctgggg gcggtgacgc tgttctcaac ggatcgctcc ctcaccggcc aggacggtgt
      661 cagcctcgcc cctggagcct cggacggcgc cgacccgcct catgagctcg cccgccgcct
      721 gttcgacgcc gatgatctca tcgaccacgt ccatgtcctg tccaacacgg tcgcggtgcg
      781 ccggcgcgaa agctgggatg aagaggcggt ggaccgcgcc cgcgacgtca tcgccaatct
      841 cttcgtccac tacggcactg ggccggcacc ttcgggagcc gacgagttcg agcagctacg
      901 ggtgcagaac tacaacgcga ccttgagcca catcagggcc cacaacgagg acctgtgggt
      961 gatgcgcgtc acccccgacc agccgatcga cccgtttctc cccggccagt acacgacgct
     1021 cgcgttgggt tactgggagc cgcgggcgga cgacgcccac gacaacctga agcctgatca
     1081 ggatcagaag atggctcgcc ggtcctattc ggtgtcgagc tcgatgatcg acgagacggg
     1141 tgagcttctc ccaccacaca ccccggatgt cgagttctac atcgtgaagg tcaagcccgg
     1201 ccaggaggag atacccgctc tcactcctcg ccttttcatg aaagacgagg gggatcgtct
     1261 ctacatgggt cgcaagttca ccggtcacta cacgctggag ggggtcagtc ccgatgacaa
     1321 catcgtcttc ctcagcaccg ggaccgggga ggccccacag aacgccatga tcgccgagtt
     1381 gttacggaag gggcacgaag ggcacatgct gaacgtcgta tgcgttcgtt accggagcga
     1441 cctcgcctac accgaacagc atgcggtatt ggtcgaccgc tattccaact acaagtacgt
     1501 caccatcacc actcgggagc ccgagaacga gggcaagaag gtctacatcc aagatctggt
     1561 cacctcgagc caaatcgaga aggagctcgg cgcccccatg gaccccgacc acacccacgt
     1621 gttcctgtgt ggcaacccgg cgatgatcgg gttgccgaaa tggaccgacg acggaatggt
     1681 cttccccgag accctcggtg tctgccagca gctgcacgag aagggcttca ccatcgatca
     1741 tcgcaaggaa cgaggcaacg tccattacga ggagtattgg acggatcgct gagcgacacg
     1801 atcgagcagg attcgagaag cgtctcccac gcatagcccg taggttcagg ggtgactcat
     1861 acaggaggta agagatgagc accaccaaga agacagacgg cttctcgacc gcagagcgtg
     1921 ccgccatgaa ggcacgcgct caggagttga aggcgcaggg ccggagcggt cagaagcagg
     1981 ccgacaacga gcaggccgtt ctcgacgcga tcgccgagat gccggatgcc gaccgcacga
     2041 tcgccgaacg catccatgcg ctcgtcaccg
//
LOCUS       DASTXO010000342         2412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_647105, whole genome shotgun sequence.
ACCESSION   DASTXO010000342 DASTXO010000000
VERSION     DASTXO010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647105"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1800)
                     /locus_tag="VFU96_07230"
     CDS             complement(<1..1800)
                     /locus_tag="VFU96_07230"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEU5113097.1"
                     /translation="MNIEKGHLFIGLEGGEAKEPLVYDSADFTTHGVIVGMTGSGKTG
                     LGITILEEALLNGIPCLVIDPKGDMGNLLLNFPDFAPADFRPWVDEAEAERQGVTADQ
                     LAADIASSWKEGLAGSGIAPERMAELRDGAEITIYTPGSGAGVGLNVLGSMMAPTLDW
                     ETEGELINDEIEAFVSSLLSLADIDADPVSSPEHILLSTIISTFWREGRDLDLATLVG
                     QVPRPPFRKLGVFDIDTFFPEKDRMALAMKLNGLLASPSFAAWLQGAPLDVGEMLTGG
                     DKVRAAIVYLTHLSDAERSFVVTLLLSKVVSWFRTQPGTSDLRALIYMDEVFGFVPPT
                     AEPPSKKPILTILKQARAHGVGMILSTQNPVDLDYKAMSNAGTWMVGRLQTENDKKRI
                     LEGLASATGAVDVAVYDRLISDLEKRQFVLSTTKVSEPTLFGTRWAMSYLAGPLTRDQ
                     VSALMKGRSAPTTVEEAVTTDVAASTQASLVTEPIGQEDVPVMPKVSEGVATVYLDPA
                     AAWAGEVGADPTGKRLTAAIAATVKLTYDDTKAGVNHQEAFEAVVFPVGPVLQPDSVR
                     EIDHDPRDFRSDPPEGAAYVLPEQKIDTKSFWTG"
     gene            complement(1849..>2412)
                     /locus_tag="VFU96_07235"
     CDS             complement(1849..>2412)
                     /locus_tag="VFU96_07235"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HEU5113098.1"
                     /translation="VTWLSPRKLLDKLSHDQDRELIAGTDFKKLAQTGNLYLFRHTTA
                     GERSRWKGRDEDRSLTKKGWREADAIAGSLADAGIERILSSPYERCVQSVKPLSKLIK
                     APVEISPLLAEEPDMDAAYALIDGLIGTNAVVCSHGDVIPALINRMMWAGLTLDSRFY
                     CSKGSIWVVGVEEGKFTNAHYRPPPEV"
BASE COUNT          428 a          766 c          750 g          468 t
ORIGIN      
        1 gccagtccag aaggacttgg tatcgatctt ttgctccggg aggacatatg ccgccccctc
       61 cggtgggtcg gaacggaagt ctctcggatc gtgatcgatc tccctgaccg aatcgggctg
      121 caacacgggc ccgaccggga agacgacagc ctcgaatgcc tcctggtgat tgacaccagc
      181 cttcgtatcg tcgtatgtca gtttgaccgt tgctgcgatg gcagccgtta gccgcttgcc
      241 tgtcgggtcc gcgcccacct ccccggccca ggccgcagcc gggtcgagat acacagttgc
      301 cacaccctcc gacaccttgg gcattaccgg cacgtcttcc tgcccgattg gctcagtgac
      361 taaggaagcc tgggtcgaag cagcgacgtc ggtcgtcacc gcttcctcca cggtcgtggg
      421 cgccgaccgt cccttcatca gggctgacac ctgatccctg gtcagcgggc cggccaggta
      481 cgacatggcc caacgcgtcc cgaacagtgt gggctcggag acctttgtcg tgctcagcac
      541 gaattggcgt ttctccaggt ccgagatgag cctgtcgtag accgcgacgt cgacggcgcc
      601 ggtggcgctg gcgaggcctt cgagaattcg cttcttgtcg ttctctgtct gcagacgccc
      661 gaccatccag gttcccgcgt tggacatcgc cttgtagtcc aggtcgaccg ggttctgggt
      721 ggagaggatc atccccacgc cgtgagctcg tgcctgcttg aggatggtca ggatcggctt
      781 cttcgaggga ggctcggcgg tcgggggcac gaatccgaac acctcatcca tatagatcag
      841 ggcgcggagg tcggaggtcc cgggctgggt ccgaaaccag gagaccactt tggagaggag
      901 aagggtcacc acgaacgacc gctcggcgtc cgacagatga gtcaggtaga cgatggctgc
      961 cctgaccttg tcgcccccgg tgagcatctc gccaacgtcg agtggagctc cctgtagcca
     1021 ggctgcgaac gacggcgacg ccaacaaccc gttgagcttc atcgcaagtg ccatgcggtc
     1081 cttctcgggg aagaacgtgt cgatatcgaa cacacccagc ttgcggaacg gcggtctggg
     1141 cacctggccc accagtgtgg ccagatcgag atcccgacct tcacgccaga aggtggagat
     1201 gatcgtggac aaaaggatgt gctccgggct cgacaccggg tcggcgtcga tgtccgccag
     1261 ggagaggagc gatgacacga aagcttcgat ctcgtcgttg atcagctcgc cttccgtctc
     1321 ccaatcgagt gtgggcgcca tcatcgagcc gaggacgttg agcccgactc cggccccgga
     1381 gcccggcgtg tagatcgtga tctcggctcc gtcccggagt tcggccattc tctccggggc
     1441 gatcccggat cccgccagac cctccttcca ggagctcgca atatcggcag ccagctggtc
     1501 cgcggtgaca ccctgtcgtt ccgcctcggc ctcgtccacc cagggccgga agtccgccgg
     1561 ggcaaagtct gggaaattga ggaggaggtt tcccatgtct cctttggggt cgatgacgag
     1621 gcaggggatg ccgttgagca gcgcttcctc gaggatcgtg atgccaagac cggtcttgcc
     1681 cgagccggtc atgccgacga tcacgccatg cgtggtgaaa tcggcgctgt catagacaag
     1741 tggctccttc gcctcgccac cctccagccc gatgaacaaa tgtcccttct cgatgttcat
     1801 ggaccggaat atatgccagc ggtcgggccg gctccccgcc tcggcggatc agacctcggg
     1861 tggcggccgg taatgggcgt tggtgaattt cccttcctcc accccgacca cccagatgga
     1921 gcccttggag caatagaacc gggagtcgag ggtgagcccg gcccacatca tccggttgat
     1981 gagggcgggt atgacgtcgc cgtgagagca gacgacggca ttcgtcccga tcagcccatc
     2041 gatcagcgcg taggcggcat ccatatcagg ctcctcagcc aagagtggcg atatctcgac
     2101 cggcgccttg atcagcttgg acaaaggctt gaccgactgc acgcatctct cataggggct
     2161 ggagaggatc cgttcgatgc cggcatcggc caaagagcct gcgatggcat cggcctcccg
     2221 ccaacccttc ttggttagtg agcgatcctc atcgcggcct ttccacctgg agcgttctcc
     2281 ggcggtggtg tgcctgaaca agtacaggtt cccggtttga gccagcttct tgaagtcggt
     2341 ccccgcgatc agctcccggt cctgatcgtg gctgagcttg tccagcaact tgcgtggtga
     2401 cagccacgtc ac
//
LOCUS       DASTXO010000343         5452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1494405, whole genome shotgun sequence.
ACCESSION   DASTXO010000343 DASTXO010000000
VERSION     DASTXO010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494405"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="VFU96_07240"
     CDS             complement(<1..535)
                     /locus_tag="VFU96_07240"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU5113099.1"
                     /translation="MITLADIEEARARFEGNVTETPLVWSQSFSRLTGRDVYLKAECM
                     QRAGSFKIRGALNVMTRLDGKPVVAASAGNHAQGVALAAAFTGSPCTLFMPETAPIPK
                     VKATEDYGADVRLVGISLADAVEAAVEHSRQTGAHFVHPYDDPLIISGQGTLGLEILE
                     QLPETGTVVIPTGGGGLL"
     gene            648..1100
                     /gene="rplM"
                     /locus_tag="VFU96_07245"
     CDS             648..1100
                     /gene="rplM"
                     /locus_tag="VFU96_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017598565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU5113100.1"
                     /translation="MNKTYSPKPGDVERAWLVVDASDLPLGRLASEIATVLRGKHKPT
                     YAPHVDGGDFVIVVNAERVAVTSDKSQTKIYYRHSGYPGGIKAETFDRLRERRPEAII
                     ERAVNGMLPKTKLGRQMARKLKVYAGPDHPHVAQKPQVLELKIRKVEL"
     gene            1100..1492
                     /gene="rpsI"
                     /locus_tag="VFU96_07250"
     CDS             1100..1492
                     /gene="rpsI"
                     /locus_tag="VFU96_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014262207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU5113101.1"
                     /translation="MAKPLVQATGRRKASVARVRLVDGSGQVILNGRPLEDYFPTMAT
                     RLRVMEPLQITQTQGRYDVHATLEGGGQTGQADALRLGIARALIVLDEELRPVLKKAG
                     LLTRDARVVERKKYGLRKARRAPQYTKR"
     gene            1495..2850
                     /gene="glmM"
                     /locus_tag="VFU96_07255"
     CDS             1495..2850
                     /gene="glmM"
                     /locus_tag="VFU96_07255"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009710963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU5113102.1"
                     /translation="MADLRRLLDEGKPIFGTDGVRGVAGAELSAELALLIGRAAGSYL
                     RGGPVVVGRDTRRSGEMLSSALQAGFHSMGVDTVDVGILPSGGISYLTATSGATMGAI
                     VSASHNPAEDNGIKLLAARGTKLPDDVERELEDRLRSPEARSKVGPDIGTRFVDSDAL
                     ERYVDHLASVSKYNLSSVEIALDTANGAAFKAAPLLFNRLKADVHVLADAPDGTNIND
                     RCGATHPEMLAREARGRIGLSFDGDADRLIAVDEDGRVANGDVILAIVARYMKAQGSL
                     KKNLVVATVMSNLGFRKSMAEADIELIETKVGDRYVMEAMLEHKAILGGEQSGHIIFL
                     DKGHTGDGLLTAVRLLDVVAGTGKELRELRAEAITEYPQILQNVPVGRGASLDDAGAL
                     WDEVRAVETELGAEGRVLVRASGTEPLIRVMVEAPSEEAARRYADRLSSVTQTSMGKE
                     G"
     gene            3177..5003
                     /gene="glmS"
                     /locus_tag="VFU96_07260"
                     /pseudo
     CDS             3177..5003
                     /gene="glmS"
                     /locus_tag="VFU96_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
     gene            5042..>5452
                     /locus_tag="VFU96_07265"
     CDS             5042..>5452
                     /locus_tag="VFU96_07265"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU5113103.1"
                     /translation="MKLGIELPEDRPVVALGAHADDIEIGAGGTLLQLSIDHPDSRFL
                     FAVAASDTERSGEAIASARDLLGDRASVQVGGFEDGLLPYTDPAGVKRWLRSVVESIR
                     PAVVFSPWSGDEHQDHRLMGQLAWQVFRGSTILEY"
BASE COUNT          995 a         1699 c         1803 g          955 t
ORIGIN      
        1 cgaggaggcc gccgccccct gtcgggatga ccacggtccc ggtctcgggc agctgctcca
       61 ggatctcgag cccgagtgtg ccctgcccgg agatgatcag agggtcgtcg tagggatgga
      121 cgaaatgcgc gccggtctgt ctggagtgct cgaccgcagc ttcgacggcg tcggccagtg
      181 agatccccac cagcctcaca tctgccccat agtcctcggt ggctttgacc ttggggatgg
      241 gcgccgtctc tggcatgaac agtgtgcatg gtgatcccgt gaacgctgcg gcgagggcga
      301 cgccttgggc atgattgccc gcggaggcgg ctacgacggg cttgccgtcg agtctggtca
      361 ttacattcag cgccccgcgg atcttgaacg acccggcccg ctgcatgcac tccgccttga
      421 gatagacgtc acgtccggtg agacgactga aggattggga ccagacgagc ggcgtctcgg
      481 tgacattccc ctcgaaccgg gcccgcgcct cctcgatgtc ggcgagggtg atcaacggga
      541 ttggcctagc ggtgacagtc ggcgtagcat atgcgggctc cctgatcggg agccgtttcc
      601 ccaaacgctc cccggtatct tcaacctgcc gaggattact catgaccatg aacaagacct
      661 attcgcccaa gccgggcgat gtcgagcgcg cctggctggt ggtggacgcc agcgacctcc
      721 cgttgggccg tctcgcctcc gagatcgcca cggtgctccg tggcaagcac aagccgacct
      781 acgccccaca cgtcgatggt ggtgatttcg tcatcgtggt gaacgccgag agagtggccg
      841 tgacctcgga caagagccag accaagatct actaccgcca ctccggctat ccgggaggca
      901 tcaaggcgga gaccttcgac cgcctccgcg aacgccgccc cgaggcgatc atcgagcgtg
      961 ctgtcaacgg catgctcccc aagaccaagc tgggccgcca gatggcccgc aagctgaagg
     1021 tctacgccgg gcccgatcat cctcatgtcg cccagaaacc ccaggttctg gagctgaaaa
     1081 tccgaaaggt ggaactctga tggccaagcc actcgtccag gccacaggtc gtcgcaaggc
     1141 ctcagtggcc agagtccgtc tcgtcgacgg ctcgggccag gtgatcctca acggccggcc
     1201 cctcgaggac tatttcccga ccatggccac ccggctcagg gttatggagc cgctccagat
     1261 cacccagacc cagggccgtt atgacgtcca tgccactctc gagggcgggg ggcagaccgg
     1321 tcaggccgat gcgctgcgtc tcggaatcgc aagggccctc atcgtgctcg acgaggagct
     1381 tcggcccgtt ctcaagaagg ccgggctgct cacccgtgac gctcgggttg tcgagcgcaa
     1441 gaagtacggg cttcgcaagg ctcgtcgggc gcctcagtac acgaagcgct gacattggcc
     1501 gacctccgtc gtctcctcga cgagggcaaa cccatcttcg gcaccgatgg cgtgcgcggg
     1561 gtggctggcg ccgagctctc cgccgaactg gcccttctca tcgggcgcgc cgccggctcc
     1621 tatctccgcg gtggcccggt ggtggtgggc cgcgacacga ggcgctccgg ggagatgttg
     1681 tcttctgctc tccaggccgg gtttcattcg atgggtgtcg acaccgtcga cgtcggcatc
     1741 ctccccagcg gggggatcag ctatctcact gcgacgtcag gtgcgacgat gggtgccatc
     1801 gtctccgcgt cgcacaaccc ggcggaggac aacgggatca agctgcttgc cgcccgcggc
     1861 accaagcttc ccgacgacgt ggagcgggag ctcgaggacc gactgcgctc tcctgaggca
     1921 aggtccaagg ttggccccga catcgggacc cggttcgtcg atagcgatgc cctcgagcgg
     1981 tacgtcgatc accttgcttc ggtctccaag tacaacctga gcagcgtcga gatcgcactc
     2041 gacaccgcca acggcgccgc cttcaaagcg gcgccgctgt tgttcaacag actcaaggct
     2101 gatgtacatg tcctggccga cgcccccgat ggcaccaata tcaacgacag gtgcggggcg
     2161 acacatcccg agatgctggc gagagaggcc cggggccgga tcggcctgtc attcgatggg
     2221 gacgccgacc ggctgatcgc cgtcgacgag gacggccggg tggcgaatgg cgatgtcatc
     2281 ctcgccatcg tcgcccgata catgaaggcc cagggctctc tcaagaagaa cctggtggtg
     2341 gcaaccgtga tgtcaaacct cggtttccga aagtcgatgg cggaggccga catcgagctg
     2401 atcgagacca aggtcggcga ccgctacgtg atggaagcca tgctcgagca caaggcgatc
     2461 ctgggtggcg agcagtcggg ccacatcatc ttcctcgaca agggccacac cggggatggt
     2521 ctgcttaccg cagtgcggct tctcgacgtg gtcgccggca ctggaaaaga gctgcgggag
     2581 ctcagggcgg aggcgatcac cgagtatcca cagatcctcc agaacgtgcc ggtgggccgg
     2641 ggggccagcc tcgacgacgc cggggcactc tgggacgagg tgcgcgccgt cgagaccgaa
     2701 ttgggagcgg agggcagggt cctagtccgg gcatcgggca ccgagcccct gatcagggtg
     2761 atggtagagg ccccttccga agaggcggcg aggcgctacg ctgatcgctt gtcgagcgtc
     2821 acacagacgt cgatggggaa ggagggataa acaccgatag caaacatctg cgtgcgatca
     2881 actaacagcg cccggcctca ttcacggtga aatctgggga ggacctcaga ctcccgcagt
     2941 gaccccccgc agtgaggtga cgaggaggaa ggtcatcgaa atcttcggcg gatgccttcc
     3001 cggccagctc aggtcgttag ggatgggagc aaaacccgga agcgattccg gaacagagac
     3061 ccactcatga gcgccgattg atcggccccc gcacgcatca cccgcgttct cgatgcaggg
     3121 cgtcatgcct acgtgacgcg ttgcatccga acaaatagct atagcgagga tgggatatgt
     3181 gcggaatcgt tggatatgtc gggccgaagc ctgcgatcga cgtcttgatg ccgggccttg
     3241 cccggctgga atatcggggg tatgactcgg ccggagtggc cctcaaccac ggcggatcgc
     3301 tgaccgttct caagcaggcc ggtcggattg ccgacctcca agggctggtg gatcgggagg
     3361 atctggcgga ggcccatacc gggattggtc acacccgctg ggccactcat ggtgcgccga
     3421 acacgggcaa tgcccacccg catctcgatt gcactcggga cgtggtggtg gttcacaacg
     3481 ggatcatcga gaactgggtc gatctcaagg ccaagctcgt cgacaacggg cacgaattcg
     3541 agagcgacac ggacaccgag gtcatcgccc acttgatcga ggacatggcc gacatttctc
     3601 tagccgacgc ggtccgcaac gtgatgaatc aggccgacgg cgccatggcc ctggtggtca
     3661 tgcggaattc cgagcccgat gtcttggtcg gggcaaggcg tgggtaaccc ttggtcgtgg
     3721 gccggggcaa gggtgagaac ttccttgcat cggatatccc cgcattcctc gagcacaccc
     3781 gtcacatggt gatcgtggag gacgaccggg tcgtcgagat ccgcgccgat caggtgaccc
     3841 tgaccgatct cgccggaaac ggcatgccac tcgacgagcg tctcgtggag tgggacttcg
     3901 aagcggcgga gaaaggtggg ttcgacacct tcatgctcaa ggagatcttc gagcagccgc
     3961 acgccatcac cgacaccctt gccggccgcc tcgagttccc gggtcgggtg gtgctcgacg
     4021 aattgaccct cgatcccgag atcctgcgcg aggtcgacaa ggttttcgtc gtggcgtgcg
     4081 gcacctcata tcacgcggcg atgatggcca aatatgccat cgagcgttgg gcgcgccttc
     4141 ccgtggagat cgacatcgct tctgagttca gataccgcga ccccgtgctc gacgatcgct
     4201 ccctcgtcat cgggatcagc caatctggcg agaccatcga cactctggcc gcggttcggt
     4261 acgccagggc acagggttcg gccgtgatcg gcgtgtcgaa tgtggtggag tcagctctcg
     4321 cgcgtgagtc ggacgccgtc ctctacacca gggccggccc cgagatcggt gtggcggcta
     4381 ccaagacctt caccactcag ctcgtggcga tgcagctgct cggcctgtat ctcgcccagg
     4441 ttcggggcgc actcgacccc ggggccatcg agcttcaggt gaaggagctc ctccgcctcc
     4501 ccgaccaggt cgaggcggta ctggctggcg ccgaggagtt gagggccatg gcggagccct
     4561 ggtcggagac ccgggactgg ttcttcctcg gccgttctgg cgatttcccg gtagccatgg
     4621 aaggtgctct caagctgaag gagatcgcat acgtccgggc cgagggatat gcggccgggg
     4681 agatgaaaca cggcccgatc gcgctcatcg aaccgggcgt cgtggtggtc ggtgtcgcca
     4741 cagacagcca cgtccaggcc aagaccctgt ccaacatggc ggagatgaag gcgaggggtg
     4801 ggacgatcgt gttggtcgcc gaagagggag acgatgtcgg ccacgtcgcc gaccatgtga
     4861 ttcgggtccc ggcggggcct gacctgctct gcactgtcac ggccgtggtg ccgctccaac
     4921 tactcgccta ctacgtggcg acagctaagg gaatggacgt ggacaagccg cgcaatctgg
     4981 ccaagacggt gacggtggaa tagctggacg acgttccgga gactgaggaa ggagaaggcc
     5041 ggtgaagcta ggcatcgagc tccccgagga tcgcccggtg gtggctttgg gcgcccatgc
     5101 agacgacatc gagatcggtg ccggcggcac tcttctccag ctgtctatcg accaccctga
     5161 cagccggttc ctgttcgctg ttgctgcatc tgacaccgaa cggtccggcg aagcaatcgc
     5221 cagcgcccgt gatctgctgg gggatcgagc atcggtgcag gtaggggggt tcgaggatgg
     5281 ccttctccct tacaccgatc cggcgggagt caagcgttgg ctgcgctcgg tggtcgagtc
     5341 gattcgacct gccgtggtgt tctccccttg gtccggtgac gagcaccagg atcatcggtt
     5401 gatgggtcaa ctggcgtggc aggtctttcg gggctcgacc atccttgagt ac
//
LOCUS       DASTXO010000344         2950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_204933, whole genome shotgun sequence.
ACCESSION   DASTXO010000344 DASTXO010000000
VERSION     DASTXO010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204933"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="VFU96_07270"
     CDS             complement(<1..1146)
                     /locus_tag="VFU96_07270"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEU5113104.1"
                     /translation="MRGITISFPGVLALDNVDFFLRPGEVHSLMGENGAGKSTLIKAL
                     TGVYAIDSGSIDVFGTSRVFHSTFEAEAAGISTVYQEINLCTNLSVGENVMLGHEPRR
                     RGRIDWKAVHRLAAEHLEGLGIAIDTRSLLSNHSIAIQQLVAISRAMVLDAKVFVLDE
                     PTSSLDRGEVEQLFTVIRDLRAKGAAILFVSHFLDQVYEISDRITVLRNGKLVGEYVV
                     KDLPRDELVTKMIGRQLDDLEAISETAAREIDRTATPVLNASDLGKRGVLEPADLDVF
                     QGEIVGIAGLLGSGRTELVRLLYGADRADSGQIEVGGVPAKMTSPRHAIDRGVAFSSE
                     DRRAEGIVSDLTVAENIVLGIQARAGIRRIRRAKQESVVSEYMQALDV"
     gene            complement(1294..2313)
                     /locus_tag="VFU96_07275"
     CDS             complement(1294..2313)
                     /locus_tag="VFU96_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015253084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5113105.1"
                     /translation="MRLRERKRQFLALLTVLAVAIAACGGDGSSDTTAGGEGAATTQG
                     EASGELTTVGFAQTGSESGWRSANTESMNAAFSEENGFELIFNAADNDPAAQIAAVRS
                     FINQGVDAIVIAPIVEDGWEDVLQEAADAGIPVILEDRTVTAPEDLYATWVGLDFRNE
                     GVMAGEWAAETFGDTPTNMVVLEGTTGSAPANDRAEGFDEAIADTAIEKIDSQTGDFT
                     RDGGKTVMEGFLQAHGVDGIDLVYAHNDDMALGAIEAIEAAGGVPGEDIQIVSIDAVH
                     DGMQALIDGKINYIVECNPLLGELAAGLVNDVLAGTEVEKRYYVEDQTFTQEQAEEAL
                     PDRLY"
     gene            2671..>2950
                     /locus_tag="VFU96_07280"
     CDS             2671..>2950
                     /locus_tag="VFU96_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HEU5113106.1"
                     /translation="MINNRPDVAPDTRERVEDAIASLGYRPSAVARTLVARRSNAIGV
                     LLAGMGYTGTTLTLAGIVDEAANLGLTVLIAELPEGDSLDDPRPAISAL"
BASE COUNT          513 a          933 c          924 g          580 t
ORIGIN      
        1 cacatcgagc gcctgcatgt actcggaaac gaccgactcc tgcttggcgc ggcggattct
       61 ccggatgccc gcgcgcgcct ggatgccgag cacgatgttc tcggcgacgg tcaggtcgga
      121 cacgatgccc tcggcgcgtc ggtcttccga cgagaaagcg accccgcggt cgatggcatg
      181 gcgcggtgac gtcatcttcg ccggcacgcc ccccacctcg atctggcccg agtcggcgcg
      241 gtcggcgccg tacagaagtc gcacgagctc ggtgcgaccg gatccgagga gcccggctat
      301 gcccacgatc tcaccttgga atacgtcgag gtccgccggc tccaagaccc cgcgcttgcc
      361 caggtcagac gcgttgagga ccggagtcgc ggtacggtcg atctcacggg ccgccgtctc
      421 ggagatggct tcgaggtcgt cgagttggcg accgatcatc ttggtcacca actcgtcgcg
      481 gggcaggtcc ttgacgacgt actcgcccac gagcttgccg ttgcggagaa ccgttatgcg
      541 gtccgagatc tcgtagacct ggtcgaggaa gtggctcacg aagagaatgg ccgcaccctt
      601 ggcccgcaga tcacggatca ccgtgaacag ctgctcgacc tcaccacgat ctaggctgga
      661 cgtgggctcg tcgaggacga agaccttggc gtccagcacc atggcccggc tgatcgcgac
      721 cagctgctgt atggcgatcg agtggttgga gagcagcgat cgggtgtcga tggcgatgcc
      781 gagaccttcg aggtgctcgg cggccaatcg atgcacggcc ttccagtcga tgcgtccgcg
      841 cctgcggggc tcgtgaccga gcatcacgtt ctcgcccacg gagaggttcg tgcacaggtt
      901 gatctcctgg tacacggtcg agatacctgc tgcctcggcc tcgaaggtgc tgtgaaagac
      961 ccggctggtg ccaaacacgt cgatcgaacc actgtcgatg gcatagacgc cggtcaaagc
     1021 cttgatgaga gtcgactttc ctgcgccgtt ctcacccatg agggagtgca cttcaccggg
     1081 ccggaggaag aagtccacat tgtcgagggc gagcacgcca ggaaagctga ttgtgatccc
     1141 ccgcatgtcg acgattgggg ggccggcggt gtccgaggcc aaggtcacag cttcactctc
     1201 gatctttgat ggagtgccca accgggagcc ggtgacgaac ccgcccccgg tcagacactc
     1261 gcaagctatc tactcgggat gatcccgtga agatcagtac agccgatcgg gaagagcctc
     1321 ttcggcctgc tcctgggtga aggtctgatc ctccacgtag tagcgctttt cgacctcggt
     1381 gccggcgagc acgtcgttga ccagacctgc tgcgagctca ccgaggagag ggttgcactc
     1441 cacgatgtaa ttgatctttc catcgatgag ggcctgcatg ccgtcgtgga cggcgtcgat
     1501 ggagacgatc tggatgtcct cgcccggaac cccgccggca gcttcgatgg cctcgatggc
     1561 accgagcgcc atgtcgtcat tgtgtgcgta gaccaggtcg atgccgtcga caccatgggc
     1621 ctggaggaag ccctccatca cggtcttgcc gccgtcacgc gtgaagtcgc cggtctgcga
     1681 gtcgatcttc tcgatcgcgg tgtcggcgat ggcctcgtcg aagccctcgg cacgatcgtt
     1741 ggcaggggcc gaacctgtcg tgccctcgag caccaccata ttggtgggtg tgtcgccgaa
     1801 cgtctcggcc gcccactcgc cagccatgac accttcgttg cggaagtcca gacccaccca
     1861 cgtggcgtac aggtcctccg gagccgtcac ggtgcggtct tccagaatga ccggaatgcc
     1921 cgcatcggcc gcctcctgca gcacgtcttc ccatccgtcc tcgacgatcg gtgcgatgac
     1981 gatggcgtcg acgccctggt ttatgaagct gcgaactgcg gcgatctggg ccgccggatc
     2041 gttgtcggct gcgttgaaga tcagctcaaa gccgttctcc tcggagaaag ccgcattcat
     2101 cgactcggtg ttggccgaac gccagcctga ctccgacccg gtctgggcga agcccactgt
     2161 cgtgagctct ccgctcgctt ctccttgggt ggtggcggct ccctcgccgc ccgcggtggt
     2221 gtcgctcgag ccgtcgcctc cgcatgcggc aattgccact gcgagcaccg tcaacagagc
     2281 cagaaactgc cgtttccgtt ctctcaatct catctcgttc ctcctcgatg atcggggttt
     2341 ccgcttttcc gcctagtcga tgaacactgt ctctctctgc tggttgatgg tctcctcctg
     2401 tacttggctc agtggtgggt tcttggcgat acgactgccg ctatgttatc gttaacggga
     2461 acgctaacat agcgggagaa tcctgtctac tcaatcgagt ttccacactg ttcacggtcc
     2521 cgttatcgat aacatgggat cgaagacggg gtgggaggcc gggtgttcga gtccaccacg
     2581 aggagccaac tgatgctggg gtcgcgtcac cgagtcacga tcaaggatgt ggctgcgata
     2641 tgtggtgtgt cgacacagac ggtttcccgg gtgatcaaca acagacccga tgtggcgccc
     2701 gacacacgag agcgagtgga agacgcgatc gcgagtttgg gctacaggcc gagtgcggtt
     2761 gcccgaaccc tcgtggccag acgcagcaac gcgatcggtg tccttctcgc cgggatgggg
     2821 tacaccggca ccactctgac cctggccggc atcgtcgatg aggcggccaa cctcgggctg
     2881 acggtgctga tcgcagaact tcccgaaggc gacagcctcg atgatccgcg tcctgccatc
     2941 tcagccctgg
//
LOCUS       DASTXO010000345         2058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_831321, whole genome shotgun sequence.
ACCESSION   DASTXO010000345 DASTXO010000000
VERSION     DASTXO010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831321"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1242
                     /locus_tag="VFU96_07285"
     CDS             <1..1242
                     /locus_tag="VFU96_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU5113107.1"
                     /translation="GMRGRPLLYTSYEADQGLLNTTTKKVMLGLFLLVLVLIPFQVIP
                     SLRFLGEDDWLRLQVTTAIFVIGALGLNLLTGLAGQVSLGHAFFMGVGAYTAAWLGGP
                     VDGPLLGLGLPIWIWLPAAGIVAALIGVAVGPTAVRVRGLYLAIVTLGLVFIGDYVFR
                     NWESLTGGAQVGRDFPPLVFRWWKEDPPVIDFTTPSIWFGVELSGLAKTFFLLAMLAG
                     IFTLLAKNLQRTRVGRAFMSIRDRDIAAEIMGVNEFAYKLLAFGVSSFYAGIAGALLA
                     SFVTRVIPERWDLFLSVQFIAIILIGGAGTVAGVILGSAFVVLLPRVVQDFTLWLQSV
                     ALEGEGIGSAIANVFVATTPNDFGLVNTFAGVGPGLSVAQLNLVLYGLLIIGFLIFEP
                     LGLFGIWLRIRNYWKGWPFTY"
     gene            1296..>2058
                     /locus_tag="VFU96_07290"
     CDS             1296..>2058
                     /locus_tag="VFU96_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5113108.1"
                     /translation="MRRKPKWLALLLVLGLVLAACGDGDGGETTTTAAEEEEATTTAA
                     PEEEPTTTAGEAAGGDVATDVGVDLEAGTITIGLLSDLSGPFAPLVTVINTGHELYWA
                     SVNEAGGINGLNVELAVRDTAYVPDTHVQMYEELKSDVVAFGHSTGSPHTMAINPSLQ
                     ADGILAIPLTWYSGWSDPAINANLVPHGTPYCIEAMNMIEYLTLQDPEATTIAIATNG
                     GDYGLDSSAGAVMVAEALGLEVVYDGSGVIVATDPA"
BASE COUNT          340 a          653 c          652 g          413 t
ORIGIN      
        1 ggtatgagag gccgtcccct tctgtacacg agttacgagg ccgaccaagg gctgctcaac
       61 accacgacca agaaggtgat gctcggcctt ttccttctcg tcttggtctt gatccccttc
      121 caggtcatcc cgagtctgcg cttcctcggc gaagacgatt ggctcaggct ccaggtgacc
      181 accgcgatct tcgtgatcgg cgccctcggg ctgaacctcc tcacgggtct ggccggccag
      241 gtgtcgttgg ggcacgcgtt cttcatgggt gtcggggcat acaccgccgc ctggctcggc
      301 ggcccggtgg acggcccgct cctcggcctc gggcttccga tctggatctg gctacccgcc
      361 gccgggatcg tcgccgcgct gatcggggtg gcagtcggcc caacggcagt gagggtcagg
      421 ggtctctacc tggccatagt cacgctcgga ctcgtcttca tcggggacta cgtcttccgc
      481 aactgggaat cactgaccgg cggggcacaa gtaggccggg attttccacc gctcgtcttc
      541 cgatggtgga aggaggaccc gcccgtgatc gacttcacga cgccgagcat ctggttcgga
      601 gtcgaattgt cgggcctcgc caagaccttc tttctcctcg cgatgctcgc cgggatattc
      661 accttgctgg ccaagaactt gcagcgaacc cgggtcgggc gggcgttcat gtcgattcgg
      721 gaccgcgata tcgccgctga gatcatgggg gtcaacgagt tcgcatacaa gctgttggcc
      781 ttcggtgtct cctcgttcta tgccgggatc gccggggcct tgctggcctc gttcgtcacc
      841 cgggtcatac ccgaacgctg ggacctgttc ctgtcggtcc agttcatcgc catcatcttg
      901 atcggcggcg ccggaactgt ggccggcgtc atcctcggtt ccgctttcgt ggtgctcctg
      961 cccagggtcg tccaggactt caccctttgg ctccagtccg tcgccctgga aggggagggg
     1021 atcggctcgg cgatcgccaa cgtcttcgtc gctacgaccc ccaacgactt cgggttggtc
     1081 aacaccttcg ccggcgtcgg gccgggcctg tcggtggctc agctgaacct cgtcctatac
     1141 ggtctactga tcatcggctt tctcatcttc gagccgctcg gcttgttcgg catctggctt
     1201 cgcatacgca actattggaa gggttggccg ttcacctatt gaacggtcac gacagggctc
     1261 agtgccctca cgagaaacag aggaggaagg aacgaatgag aagaaagccc aagtggctgg
     1321 ctctgctctt ggtccttgga ctggtcctcg ccgcctgcgg tgacggtgac ggcggtgaaa
     1381 cgaccaccac cgcagccgaa gaggaggaag ctacgacgac ggctgcgccg gaggaggaac
     1441 cgacgaccac ggccggcgaa gcggccggag gggacgtcgc cacggatgtc ggcgtcgatc
     1501 tcgaggccgg caccatcacc atcggtctcc tatccgacct gagtgggccg ttcgccccgc
     1561 tggttacggt gatcaacacc gggcacgagc tctattgggc ctcggtgaat gaggcaggcg
     1621 ggatcaatgg tctcaatgtc gagctggcgg ttcgtgatac cgcgtacgtc cccgacacac
     1681 atgtgcagat gtacgaggag ctcaagtccg acgtcgttgc gttcggacat tcgacagggt
     1741 cgccccacac catggcgatc aacccgtcgt tgcaggcgga tgggatcctg gccatccctc
     1801 tgacctggta ctcgggttgg tccgacccag ccatcaacgc caacctcgtg ccgcacggga
     1861 cgccgtactg catcgaggcg atgaacatga tcgagtacct gacgctccag gatcccgagg
     1921 cgacgaccat cgcgatcgca acgaatggtg gcgactacgg gctggattcg agcgctggcg
     1981 ccgtgatggt tgcagaagcc ctcggcctcg aggtggtcta cgacggcagc ggcgtgatcg
     2041 tggccaccga tccggcca
//
LOCUS       DASTXO010000346         4338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1383966, whole genome shotgun sequence.
ACCESSION   DASTXO010000346 DASTXO010000000
VERSION     DASTXO010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383966"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /locus_tag="VFU96_07295"
     CDS             <1..1147
                     /locus_tag="VFU96_07295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEU5113109.1"
                     /translation="DVELARAAARGLEDLDGATAESELVCVAPATAFLETCELALAEA
                     DTGAEAAEAMVFGMATFGLDPHSVYLDPEAVQLLEEEQAGEIQGIGALVTAEDSATGE
                     ECNVISDTCRIQIVSTIDGTPADTAGLQPEDLILGVDGTDISGWTVDEVTAMVRGSAG
                     SDVVLTIGRDGDVFDMTITRGLFVVPTVVSEVVGETGYIGLNTFTDNADELFLEHVQA
                     LLDQGVESLVLDMRNNPGGLLDTAIEVASAFLPDGDVVVTKGPDSSTSYPTSGDPIVP
                     EDMRVIVIVNEGSASASEVVSAVLQERGRATVVGENTFGKNTVQQRFALSNTGALKLT
                     IARWLTPGGHDFGSVGVTPDVSADIEADLPVETVVQEALAASVTLEG"
     gene            complement(1170..1805)
                     /gene="msrA"
                     /locus_tag="VFU96_07300"
     CDS             complement(1170..1805)
                     /gene="msrA"
                     /locus_tag="VFU96_07300"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEU5113110.1"
                     /translation="MFGRRKTEMPEAGAALPGRATAVDVPPAHFINGNPLVGPFPGDT
                     ETAVFGMGCFWGAERRFWKMDGVYTTAAGYAGGHTPNPSYEEVCTGRTGHSEVVLVVY
                     RPEEVSYEELLKVFWETHDPTQGMRQGNDLGSQYRSAIYTTTDAQLEIAKRSRDKYQA
                     ELTPLGFDDITTEITPLGEFYYAEDYHQQYLGKNPHGYDCHANTGIAYPVG"
     gene            complement(1826..2206)
                     /locus_tag="VFU96_07305"
     CDS             complement(1826..2206)
                     /locus_tag="VFU96_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU5113111.1"
                     /translation="MERVNVQSGSRFEDEIGFSRAVRVGGRVVVSGTAPIWPDGTVDP
                     DPKAQASRCLEIMLTALGEAGGRAEDVVRTRTYLLDAADANAVGEAHREVFSGIRPAS
                     TMVVINGLLDPRWKVEMELEAVLA"
     gene            2284..3453
                     /locus_tag="VFU96_07310"
     CDS             2284..3453
                     /locus_tag="VFU96_07310"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEU5113112.1"
                     /translation="MRALRSLTLLALFSTFALAVATVGHHAARAQTVGESEQEAEAAE
                     RQSEVAAGLVDTAVARRAEIEAELAVSIARINDLSSQLSAVSSDLDSLKAQIAFGDAE
                     LTGIEADIEIHAIDAYMDALVGPGVSFVNSANVEQAMVTGQVVGEVVTAGAARVNELV
                     VRREALEDLQDVYLIQQDRVAALRAEVDMEVEHLAALYDEADAAVGNAVREANAADAA
                     HREALSAVDAAQAREEERRRQEERAPGTTTPPPEEEEDTPTTTTTPETPETTDGGGGG
                     SSGGGEWDFPPSVEQWRSLVQSYFPSSRVEEALAILQCESLGDADAYNPYSGASGLFQ
                     FLPSTWASTSPQAGFGGASVFDGGANIGTAAWLANRYQELGKGYWSPWSCRRVLG"
     gene            3450..3650
                     /locus_tag="VFU96_07315"
     CDS             3450..3650
                     /locus_tag="VFU96_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113113.1"
                     /translation="MTTGYGQGVEEEWFYCLDHRTVEPADGCRAAVRIGPFPSRDEAA
                     KALETVQRRNREWDNDPEWSED"
     gene            3715..>4338
                     /locus_tag="VFU96_07320"
     CDS             3715..>4338
                     /locus_tag="VFU96_07320"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton exchanger"
                     /protein_id="HEU5113114.1"
                     /translation="METAQPDSRQQILFMALALAATLPGVFLRITGIHPDPIVAAICY
                     GLAIVGAAFILSWAAEVIQMDIAAGLALALLALIAILPEYAVDFVFTAKAGQEFAETG
                     QASQYGPLALANMTGGNQLLIGLGWPMVILIGTWRVRKTKTSPEEAEGTTATEIHLSR
                     GQSVDIAYLTIASLYGLTLFLKPTLSLFDAVVLVTIYVLYLRRLSGAP"
BASE COUNT          753 a         1459 c         1386 g          740 t
ORIGIN      
        1 cgacgtcgag ctggctcgag cggccgctcg aggtctggag gacctcgacg gggcgaccgc
       61 cgaatccgag ctcgtctgtg tcgcacccgc taccgccttc ctcgagacgt gcgagctcgc
      121 gctcgccgag gccgacaccg gcgccgaggc tgccgaggcc atggtcttcg gcatggccac
      181 cttcggcctc gatccccatt cggtgtacct cgaccccgaa gccgtccagc tcctggagga
      241 agaacaagcc ggcgagatcc agggcatcgg ggccctggtc acggccgagg actcggcgac
      301 gggagaggaa tgcaacgtga tctccgacac ctgccgcatc caaatcgtct cgaccatcga
      361 tggcaccccg gctgacacgg ccggtcttca gccggaagat ctgatcctgg gcgtcgacgg
      421 gacggatatc tcaggatgga ccgtcgacga agtcacggcc atggtgcggg gctcggccgg
      481 ctccgacgtg gtcctcacca tcgggcgcga cggcgatgtc ttcgatatga ccatcacccg
      541 agggctcttc gtggttccaa ccgtcgtgag cgaagtggtc ggagagacgg gctacatcgg
      601 gctcaacact ttcacagaca acgcggacga gttgttcctg gagcacgtac aggcacttct
      661 cgaccagggt gtcgagtcgc tcgtgctcga catgcgtaac aaccccggcg ggctcctcga
      721 cacggcgatc gaggtggcct ccgccttcct gcccgacggt gacgtcgtcg tgaccaaagg
      781 ccccgactcc tcgacctcgt acccgacttc aggcgatccg atcgtgcccg aggacatgcg
      841 ggtgatcgtc atcgtcaacg agggaagcgc ctccgcgtcc gaagtggtat cggcggtcct
      901 tcaggaacga ggcagagcga ccgtggtggg cgagaacacc tttggcaaga acaccgtcca
      961 gcagagattc gccctctcca acactggagc gctcaagctc acgatcgccc gttggctgac
     1021 tcccgggggc catgatttcg ggtcggttgg ggtcaccccg gacgtgagcg cggacatcga
     1081 ggccgacctg ccggtggaga ccgtggtcca ggaggccctg gcggcgtcgg tgacgctaga
     1141 ggggtagccg cgaccgaacg ccgggccggt caccccaccg ggtaggcgat gccggtgttg
     1201 gcatggcagt cgtacccatg ggggttcttg cccagatact gctggtggta gtcctcggcg
     1261 taatagaact cacccagcgg tgtgatctcc gtggtgatgt cgtcgaagcc gagcggtgtc
     1321 agctccgcct gatacttgtc tcgagaccgc ttcgcaatct ccagttgggc gtcggtggtg
     1381 gtgtagatgg cggatcgata ctgcgacccg aggtcgttgc cctgtcgcat tccttgcgtc
     1441 gggtcatggg tctcccagaa caccttcagc agctcctcgt aggagacctc ctccggccgg
     1501 tatacgacca gcacgacctc ggagtgcccg gtgcggccgg tgcacacctc ctcataggaa
     1561 ggattgggcg tgtggccacc cgcgtagcca gcggccgtgg tgtagacgcc gtccatcttc
     1621 cagaaccgcc gctcggcgcc ccagaagcaa cccatcccga acaccgcagt ctcggtgtca
     1681 cccgggaacg gtcccaccag tgggtttccg ttgatgaaat gggccggcgg gacatcgaca
     1741 gccgtggccc gccccgggag agcggcgccg gcttcgggca tctctgtctt tctccgtccg
     1801 aacatctgta cctccagtgt ggcagtcagg ccaagactgc ttcgagctcc atctcgacct
     1861 tccaacgagg gtcgagcaat ccattgatga caaccatcgt cgacgccggt ctgattcccg
     1921 agaagacctc acgatgcgcc tcccccacgg catttgcatc ggcggcgtcg agcagatagg
     1981 tcctggtccg aaccacgtcc tcggcccggc cgccggcctc tccaagagcg gtgagcatga
     2041 tctcgagaca gcgactggcc tgcgccttgg ggtcggggtc cacagtgccg tcgggccaga
     2101 tcggagcggt tccggacacc accactcgcc ctcccacgcg gaccgcacgg ctgaagccga
     2161 tctcgtcctc gaaccggctt cccgactgca cattgacccg ttccatagac cggccaacct
     2221 agcccaccgt ctgaatccgc acttctcgtg ccccggcctt ttcattacgc tccacgggtt
     2281 ctcatgcgtg cgctgcgctc cttgaccctc ctcgccctct tctcgacttt cgccctcgcg
     2341 gtcgcgaccg tcggccacca cgcggcgcgc gcccagaccg tcggtgagtc ggagcaggag
     2401 gccgaggctg cggagcgaca gtcagaagtc gccgccgggt tggtcgacac ggcggtggcc
     2461 cggagggccg agatcgaggc cgagctggcg gtcagcatcg ccaggatcaa cgatctgtcc
     2521 tcacagttgt ccgccgtctc gtcagatctc gacagcctga aggctcagat cgccttcggc
     2581 gacgccgagc tgacggggat cgaggccgac atcgagatac acgccatcga tgcgtacatg
     2641 gacgctctgg tcgggcccgg agtcagcttc gtgaactcag cgaacgtcga acaggcgatg
     2701 gtcaccgggc aggtggttgg agaggtggtc accgccggtg cggccagggt caacgagctc
     2761 gtcgtcagac gagaggccct cgaagacctc caggacgtct acctcattca acaggaccgg
     2821 gtggcggccc tccgggcaga agtcgatatg gaggtcgagc acctggccgc cctctacgac
     2881 gaggccgatg ctgcggtcgg caacgcggtg agggaggcca acgccgccga tgccgcccat
     2941 cgagaggcgc tcagcgccgt ggacgccgcg caggcacgcg aggaagagcg aagacgtcag
     3001 gaggagagag caccggggac cacgactccc ccgcccgagg aggaagagga cacgccgacc
     3061 accacaacga cgcccgaaac cccagaaacc accgacggcg gtgggggtgg gagcagcggc
     3121 gggggtgaat gggatttccc accctctgta gagcaatgga gatcgctggt tcagtcctac
     3181 ttcccctcga gtcgtgtcga ggaggcgctg gccatcctcc aatgcgagag cctcggtgac
     3241 gcagatgcct acaaccccta ctcgggcgcc tcgggcctct tccagttcct cccctcgacc
     3301 tgggcctcca cctcccccca ggccggcttc gggggagcga gcgtcttcga tggcggggcg
     3361 aacatcggga ccgcggcgtg gctggccaac cgctatcagg aactggggaa gggctactgg
     3421 agcccttggt cctgccggag ggtcctgggt tgacgaccgg ttacggccag ggagtggaag
     3481 aggaatggtt ctactgcctc gaccatcgaa ctgtcgagcc cgccgacggt tgccgagcgg
     3541 cggtgcgaat cggtccattc cccagcagag acgaggcggc caaggctctg gagacggtgc
     3601 aacgtcgcaa ccgagagtgg gacaacgatc cggagtggtc ggaagattga tcctccggtc
     3661 tgacccgccc ggaatcggca ataccgggcg gctctcgtag aatctcgccg ctcgatggag
     3721 actgcccagc ccgactcacg ccagcagatc ctgttcatgg cgttggccct ggccgcaacc
     3781 ctgcccggtg tcttcctccg catcaccggc attcaccccg accccatcgt cgccgcgatt
     3841 tgctacgggc tggccatcgt cggtgccgcg ttcatcctct cctgggcggc cgaggtgatt
     3901 cagatggaca tcgccgccgg gcttgccctg gccctgctcg cgctcatcgc catcctcccc
     3961 gagtacgccg tcgacttcgt gttcaccgcc aaggcgggcc aggagttcgc cgagacgggc
     4021 caagcctccc aatacggccc tctggctctg gccaacatga ccgggggcaa ccagctcctc
     4081 ataggcctgg gctggccgat ggtgatcctg atcggcacct ggcgggtccg gaagacgaaa
     4141 acatctcccg aggaagcaga ggggaccacg gcgacggaga tccacctctc gcgaggccaa
     4201 tccgtcgaca tcgcctatct caccatcgcg tccctctatg ggttgaccct tttcctcaag
     4261 cccacgcttt ccctgttcga cgcggtcgtc ctggtgacga tctatgtgct ctacctgaga
     4321 cggttgtcgg gagcgcct
//
LOCUS       DASTXO010000347         3556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1641946, whole genome shotgun sequence.
ACCESSION   DASTXO010000347 DASTXO010000000
VERSION     DASTXO010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641946"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VFU96_07325"
     CDS             complement(<1..386)
                     /locus_tag="VFU96_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012795738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5113115.1"
                     /translation="MEHVLEVRGLVHRYGDNTALAGVDLTVNSGECVALLGPNGAGKT
                     TLVRNVIGLIEGNADLIEAAGGNPRSAETRRRLGVVQQAVGFPRSLTVGEIVGGAAAR
                     RGCPPLAASQAIAEMGLAGLEKRRAA"
     gene            complement(529..602)
                     /locus_tag="VFU96_07330"
     tRNA            complement(529..602)
                     /locus_tag="VFU96_07330"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(566..568),aa:Ala,seq:cgc)
     gene            complement(636..1256)
                     /locus_tag="VFU96_07335"
     CDS             complement(636..1256)
                     /locus_tag="VFU96_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HEU5113116.1"
                     /translation="MGPDFADDARAVLDARFAAREKGIAGSRKVIRSSANGIRALHRG
                     EWDQAAGLISEAGTVLADITVALADHPDILYAGFVTDAAKEYAEARITEALFRGEPVP
                     GFVELDIDPVPFLHGLGEAVGEMRRRLLDLLRAGDVEQAQTTLEAMDRIVDLLAEMDY
                     PDGMTNGLRRTTDVSRSLVERSRSDLTATVVQERLRDDLGRLNDLA"
     gene            complement(1260..1700)
                     /locus_tag="VFU96_07340"
     CDS             complement(1260..1700)
                     /locus_tag="VFU96_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113117.1"
                     /translation="MSTSVIVGLLLIAAVWSVYLLPSVFGDRQNAPKNSTEEFDRWSH
                     LMADVQKRPYDRKAVGQRDVIRLRRRRTFAVLGFLALVTAGLALWQNSLALLLVHLFI
                     DSLIALYVAALAQMKQQRQWRLKVTHVSERPAEWDEPQIKVIAQ"
     gene            complement(1761..2240)
                     /gene="moaC"
                     /locus_tag="VFU96_07345"
     CDS             complement(1761..2240)
                     /gene="moaC"
                     /locus_tag="VFU96_07345"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEU5113118.1"
                     /translation="MADERELSHLNEAGEVRMVDVGDKPVTDREAVAEGFVVMSPDLT
                     ERFFAGDLPKGDAAAVARVAGIMAAKRTAELIPLCHPIPLTGVDLGLEPSGRGIRVIA
                     SVRTTDRTGVEMEAMTAVAVASLTIYDMVKGVERDVTIETVRLVSKSGGRSGKWERG"
     gene            complement(2230..3459)
                     /gene="glp"
                     /locus_tag="VFU96_07350"
     CDS             complement(2230..3459)
                     /gene="glp"
                     /locus_tag="VFU96_07350"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEU5113119.1"
                     /translation="MKPLEEARSEVLSSVGILGVERVPIWQAHGRVLAADVVAAENVP
                     PFANSAMDGFAVRGTDVSAAGAILEVVGDLPAGMSPDIEVGPGEAIKIMTGAPMPAGA
                     DTVARVEDTTTEGSKVTIATAVDPGTSVRPAGGDVPEGQLVFTAGTRLTPMHVGVLAT
                     IGVGHPKVFKRPRVAFMSTGDELTPPEAEGLEPGAIRDSNRPMLLALLEDAGVEPVDL
                     GIIPDDSDVLRDALTQAAAADAVVSTGGVSMGDYDVTKMVLQEDTHVDFWQVAIQPAK
                     PFAFGRIGDALFFGLPGNPVSVLVSFEQFARPSFLKLQGARHVLRPQLVATAGETLAT
                     DPEKTVFVRVLVKGLEGGSPTVVKSGGQASNVLSAAAAADGFALVPRGTATIERGDAV
                     TVEMFKWPESREWIDGR"
BASE COUNT          643 a         1197 c         1113 g          603 t
ORIGIN      
        1 ttggccgccc gacgtttctc caaaccggcc aggcccatct cagcgatggc ttggctggct
       61 gctaacgggg gacaaccccg gcgggcggcc gcaccgccga cgatctcacc gacggtgagt
      121 gaccgcggaa agccgaccgc ctgctgaacg acaccgagcc tgcgccgggt ttcggcgcta
      181 cgagggttgc cacctgctgc ctcgatgagg tcggcattgc cttcgatcaa cccgatcaca
      241 ttgcgaacga gggtcgtctt accggcccca tttgggccga gaagtgcgac gcactccccc
      301 gagttgacag tcagatcgac tccggcgaga gccgtgttgt caccgtatcg gtgaacgaga
      361 cctcgtactt cgagcacatg ttccatctac tacctccttg acaccgatca tgcggaacaa
      421 ccggtgcggc gggtagtgag atggatcacg acttcggtgt gacagatgtc acgggatctg
      481 gccagccctg cactgggccg cggaccgacc agccagagga ccttgaggtg gagctgacgg
      541 gatttgaacc cgtgaccccc tcgttgcgaa cgaggtgctc tgccaggctg agctacagcc
      601 ccttgcggga agcgaattgt accgaggggt gattctcagg ccagatcgtt cagcctgccc
      661 aagtcatccc gcagtcgttc ctgtaccact gtcgcggtga ggtcggagcg ggagcgctcg
      721 acgagggaac gggagacgtc ggtggtgcgg cggagaccgt tggtcatccc gtcgggatag
      781 tccatctcgg ctaggagatc cacgatccga tccatggcct cgagggtggt ctgggcttgc
      841 tcgacatcgc cggcccgcag aaggtcgagc aggcgccgtc gcatctcccc caccgcctcg
      901 ccaagaccat gaaggaaggg gaccgggtcg atgtccagct ccacgaaccc gggcaccggc
      961 tcgccccgga aaagggcttc ggtgatccgg gcctccgcat actccttggc ggcatcggtg
     1021 acgaacccgg catagaggat gtcgggatgg tcggcgaggg cgaccgtgat gtcagcgagg
     1081 acggttcccg cctcggagat gagcccggct gcctggtccc actcgccccg atgcaaggcg
     1141 cggattccat tggccgacga gcggatcact ttcctggacc cggcgatccc cttctcgcga
     1201 gcggcgaacc gggcgtcgag aacggctctt gcgtcgtcgg cgaaatcggg gcccatcgac
     1261 tactgggcga tgactttgat ctgcggctcg tcccactcgg cgggacgctc ggagacatgg
     1321 gtgaccttga gccgccactg acgctgctgc ttcatctgag ccagagccgc cacatagaga
     1381 gcgatcagtg agtcgatgaa gagatggacc agaagcagag ccagcgagtt ctgccacagg
     1441 gcgagcccgg cagtgaccag ggccaggaat ccgagtaccg cgaaggtgcg gcgacgacgg
     1501 agacggatga cgtcgcgttg acccacggcc ttcctgtcgt acggccgctt ctggacatcg
     1561 gccatcagat ggctccagcg gtcgaattcc tcggtcgagt tctttggtgc gttctgtcgg
     1621 tcgccgaaaa ccgagggcag gagatagaca ctccacacag cggctatcag cagtaggccg
     1681 actatgacgc ttgttgacat ttggcttacg actccgtcca cgggtgagcg tagttgccag
     1741 gaccctgtcc gatcaacgac tcaacctcgt tcccacttgc cggaccttcc acctgatttt
     1801 gagaccaacc tgaccgtttc gatcgtcaca tcgcgctcga cgcccttcac catgtcgtag
     1861 atcgtcaatg aggccacagc gacggctgtc attgcttcca tctcgacacc ggtccgatcg
     1921 gtcgtccgca cgctagcaat gacgcgaatg ccacgcccac tcggctcgag gcccaagtcc
     1981 accccggtca gcgggatggg gtgacaaagg gggatgagct ccgccgtccg cttggccgcc
     2041 atgatcccgg cgacgcgggc caccgcggcc gcatcaccct tgggcagatc gcccgcgaag
     2101 aaccgctcgg tcaggtcggg cgacatgaca acgaaaccct ctgccaccgc ctcacggtcg
     2161 gtcaccggct tgtcccccac gtcgaccatg cggacttcgc ccgcctcgtt gagatggctc
     2221 agctcacgct catcggccat cgatccactc cctcgattcg ggccacttga acatttcgac
     2281 tgtcaccgcg tcgccgcgct cgattgtcgc cgtgccacgt ggcaccaacg cgaatccgtc
     2341 tgcggcggca gcggccgaga gcacgttcga cgcctggccc cccgatttga ccacggtggg
     2401 cgagccccct tcgagaccct tgaccaggac gcggacgaac accgtcttct caggatccgt
     2461 ggcaagggtc tccccggctg tggccaccaa ctgcgggcga agcacatgac gcgccccctg
     2521 caacttgagg aacgagggcc gggcgaactg ctcgaaggag accaggaccg agacggggtt
     2581 accgggcaac ccgaaaaaca gggcatcgcc gatgcgcccg aacgcgaagg gcttggccgg
     2641 ctggatcgcc acctgccaga agtcgacatg agtgtcttcc tgcagcacca tcttggtcac
     2701 gtcgtaatcg cccatcgaca cgcctccggt agacaccacc gcatcggccg cggccgcctg
     2761 cgtgagcgcg tcgcgcagca catcggaatc gtcggggatg atgcccaggt cgacaggctc
     2821 gaccccggcg tcctcgagaa gagcgagcag cattggccgg ttcgagtccc tgatcgctcc
     2881 aggctccaga ccttctgcct cgggaggggt caactcgtcg ccggtggaca tgaacgccac
     2941 ccgcggcctc ttgaacacct tcgggtggcc gactccgatg gttgcgagca caccgacgtg
     3001 catcggggtg agacgtgtac cggcggtgaa caccaattgg ccctcgggca cgtcaccgcc
     3061 ggccgggcgg accgaagtgc ccggatccac cgcagtcgcg atggtgacct tcgagccttc
     3121 ggtagtggtg tcttcgaccc tggccacggt gtcggcgccc gccggcatcg gggcgccggt
     3181 catgatcttg atcgcctctc caggtcccac ctcgatgtcg gggctcatgc ctgcgggcag
     3241 gtcgccgacc acctcgagaa tcgctccggc ggcggagaca tctgtgccgc gcaccgcgaa
     3301 tccatccatc gcggagttgg cgaatggggg cacgttctcc gcagccacca cgtccgcggc
     3361 gaggacacgg ccatgggctt gccagatcgg gacgcgctcg actccgagaa tcccaaccga
     3421 agagagcacc tcggagcgcg cctcctccag cggtttcacc cgcttcgctc cttgagccag
     3481 gagctgaact cctgcccgaa ctcgtgctca cccagctcga ccgaagcctc gagcaacccg
     3541 atgggcgtgc ccacgt
//
LOCUS       DASTXO010000348         3004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_57778, whole genome shotgun sequence.
ACCESSION   DASTXO010000348 DASTXO010000000
VERSION     DASTXO010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57778"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="VFU96_07355"
     CDS             <1..1180
                     /locus_tag="VFU96_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5113120.1"
                     /translation="VHRETEEAERNYDLDRAAQLRHGELPELERKVKEAEEKLATEQG
                     EHRLLREVVTEDEIAGIVSRWTGIPVNRLQEGEREKLLRLDSVLHERVVGQDEAVRLV
                     ADAIIRARSGIKDPRRPIGSFVFLGPTGVGKTELAKALAAALFDTEDNMVRIDMSEYQ
                     ERHTVSRLIGAPPGYVGYEEGGQLTEAVRRKPYSVVLFDEIEKAHADVFNTLLQVLDD
                     GRLTDAQGRTVDFRNTVIIMTSNIGSQYLLEGITPAGEIKPEAREMVTSELRRHFRPE
                     FLNRVDDVVLFKPLTLEEIERVVDLMLDDVRSRLSDRSMKLEVSESAMEFIAEQGFDP
                     VYGARPLRRFISHELETRIGRALIGGEVEDGATIRVGMAQGDLVIEFENPSRAEANGG
                     "
     gene            1373..2569
                     /locus_tag="VFU96_07360"
     CDS             1373..2569
                     /locus_tag="VFU96_07360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011896037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5113121.1"
                     /translation="MKQSPAILSSIGFVSTYPPTVCGLATFTAALRGAMADERGSVED
                     LDVVSLGESRFENSRPEVVFQHVNGDRASLQQAVRILNRRDIVFFQHEYGIYGGPDGA
                     EILDFISGLEVPSVVTLHTVLDKPTLRQKRILEKIVEMAGQTVVMSDTALRRLRDRYD
                     LDSERVTMVPHGASPGLAVPGVGTGPRPVALTWGLIGPGKGLETALDAFAGVKDLEPL
                     PRYVILGKTHPKVQATQGDSYLDGLKSKAHSLGLEDVVEFDSRYLDTDSLASEIRRAD
                     VVLLPYESTEQVTSGVLVEALAAGKPVVATDFPHAVEMLGTGAGALVPHSDPEAMATA
                     LRGVFADPGVARRMATIATSIGSTLHWPVVADQYEAIVSTRIERQALMAGLSSERRAA
                     DLAKVG"
     gene            2665..>3004
                     /locus_tag="VFU96_07365"
     CDS             2665..>3004
                     /locus_tag="VFU96_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5113122.1"
                     /translation="MTDHVGLWEHARYTTPRPEHGYCTDDNARALILLSRELEPSAGL
                     IGLARVYFRFVADAALPRGGFHNRRGADESWGDDVGSDDSQGRAIWSLGTAARLGPEE
                     WMRAAALDLFH"
BASE COUNT          552 a          949 c          981 g          522 t
ORIGIN      
        1 cgtgcaccgg gagaccgagg aggcggagag gaattacgac ctggaccgcg ccgcccagct
       61 gcggcatggc gagctccccg agctggagcg caaggtgaag gaagccgagg aaaagctcgc
      121 caccgagcag ggtgagcatc gtctgctgcg cgaagtggtg acggaggatg agatcgccgg
      181 gatcgtctca cgatggaccg gcatcccggt caatcggctc caggagggcg agcgagaaaa
      241 gctcttgcgt ctcgactcgg tgctccacga gcgggtcgtc ggtcaggacg aagcggtccg
      301 tctcgtcgcc gacgccatca tccgtgcccg ttccgggatc aaggaccccc gccgacccat
      361 cggctcattc gtgttcctcg gccccaccgg ggtggggaag accgagctgg ccaaggcgct
      421 ggccgccgcc ctattcgaca ccgaggacaa catggtccgc atcgacatga gcgaatacca
      481 ggagcggcac acggtgagcc gattgatcgg ggcgcctccg ggctatgtcg gctacgagga
      541 gggtggacag ctgaccgagg cagtgcgcag aaagccctac tcggtggtgc tcttcgacga
      601 gatcgagaag gcccatgcgg acgtgttcaa caccctgctc caggtgctcg acgacgggag
      661 gctgaccgat gcccagggca ggacggtcga cttccgcaac acggtcatca tcatgacctc
      721 gaacatcggc tcccagtatc tactggaggg aataaccccg gccggggaga tcaagcccga
      781 ggcgagagag atggtgacca gcgagttgcg ccgtcacttc cggcccgagt ttctcaatcg
      841 ggtcgacgac gtcgtcctgt tcaagccgct caccctcgaa gagatcgaga gggtcgtcga
      901 cttgatgctc gatgacgtga ggagccgcct ctccgacagg agcatgaagc tcgaggtgag
      961 cgagtccgcc atggagttca tcgccgagca gggcttcgat cccgtatatg gggcccgtcc
     1021 cctgcgccgc ttcatctctc atgagttgga gacccgcatc ggccgcgcct tgatcggtgg
     1081 ggaagtggag gacggggcga ccatccgcgt cggaatggcc caaggcgacc tcgtcatcga
     1141 gttcgagaat ccttcccgag cggaggccaa cgggggctga ccgtcgcggg ccaggccatc
     1201 tcgatcgggg cttggagagg atcgctggta cactggtggc tcaaagcggc ctcggtgcca
     1261 gtctttcgtt atcccgcagg gctcgtggaa tgcagctctc cgtagccggc gaattgctgt
     1321 ggctcaacaa cagccgacgc attcaccggt cgttcacacc agggagatct gtatgaaaca
     1381 aagcccggcc atcctttcgt cgatcggatt cgtcagcacc tatccgccca ccgtttgtgg
     1441 gctggccacc ttcaccgccg ccttgcgcgg ggcgatggca gatgagcgcg gatcggtaga
     1501 ggatctcgac gtggtgagtc tcggggaaag ccgattcgag aattcacgac ccgaagtcgt
     1561 gttccagcat gtcaacggtg atcgagcctc gttgcagcag gcggttcgaa tcctgaaccg
     1621 ccgcgacatc gtcttcttcc aacacgagta cgggatctat ggcgggcccg acggcgccga
     1681 gatactcgac ttcatctccg gcctggaggt cccgtcggtc gtcacgctgc acaccgtgct
     1741 ggacaagccg acactgcgac agaagcggat tctggagaag atcgtagaaa tggcggggca
     1801 aacggtcgtc atgagcgaca cagccctgcg ccgattgcga gacaggtatg acctcgactc
     1861 cgaaagagtg acgatggtgc cccatggcgc gagcccaggg ctcgcagttc cgggtgtcgg
     1921 gaccgggcca cgacccgtcg cgctgacctg gggactgatc gggccaggga aaggcttgga
     1981 gacagctctc gacgccttcg ccggcgtgaa agacctggag ccccttcccc gttacgtgat
     2041 ccttggcaag acccacccca aggtgcaggc cacccaaggc gattcgtatc tggatgggct
     2101 gaagtcaaag gcgcactcac tgggtctcga agatgtcgtc gagttcgaca gccgttatct
     2161 ggacaccgac tccctcgctt cggagatccg acgggccgac gtcgttcttc tgccctacga
     2221 gtccacggag caagtcacct cgggcgtgtt ggtcgaggct ttggctgccg ggaagccggt
     2281 ggtcgccacg gacttccccc atgcggtaga gatgctcggc accggtgccg gcgccctcgt
     2341 cccccattcc gatcccgaag cgatggcgac cgccttgcga ggagtcttcg ccgaccccgg
     2401 tgtggcgcgt cggatggcga cgatcgcaac gtccattggc tcgaccctcc actggcccgt
     2461 tgtggcggat cagtacgaag ccatcgtctc cacgcgcatc gagcgtcagg ctctgatggc
     2521 tggcctttcc tccgagcgcc gtgccgcgga tctggccaag gtcgggtgag ctcgacgaaa
     2581 ctgccaccgc tggctttttc cggcgctgac acccttgaag acatcgaggc ccttcccgag
     2641 ccccgcttcc agcatctgcg gcgcatgaca gaccacgtgg gtctctggga gcacgcccgc
     2701 tacacgacgc cacgacccga gcacggctac tgcaccgacg acaacgcccg cgctctgatc
     2761 ctgctcagcc gtgagctcga gccctctgcg gggctgatcg gtctggctcg ggtctacttt
     2821 cggttcgttg ccgacgcagc cctcccgcga gggggtttcc acaacagacg aggagcggac
     2881 gagtcgtggg gcgacgacgt cggatccgac gactcacagg gtcgggcgat ttggagtctg
     2941 ggcaccgctg cccggctcgg accggaggag tggatgcgtg cagccgcact ggatttgttc
     3001 catc
//
LOCUS       DASTXO010000349         4116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_353218, whole genome shotgun sequence.
ACCESSION   DASTXO010000349 DASTXO010000000
VERSION     DASTXO010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353218"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VFU96_07370"
     CDS             <1..747
                     /locus_tag="VFU96_07370"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5113123.1"
                     /translation="AIGYRRVWRGTVISSFVNPVLFLTAMGLGLGTLVDSSSDDLGVP
                     YLTFVATGLMAATAMQTSAGEGSFPVMAGIKWRKGFHGAITTPLSPTDIVRGMMIWGS
                     IRLTFVLTVFALVSVAFGALDLGPALLAVPPAILTGLAFQASVTAFTVTRENEISLST
                     LFRFGIIPLFLFSGTFFPISQLPEQLQVVAYFTPLFHGVELVRKVALPGADASIVTSL
                     PIWISVVYLVAMAAAGIVLGSRWLDRRLKP"
     gene            744..1553
                     /locus_tag="VFU96_07375"
     CDS             744..1553
                     /locus_tag="VFU96_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5113124.1"
                     /translation="MSVSAPSRLTPPGRMTPFRAQRIWERNYLVYRRVWKVVITGFFE
                     PVFYLFSIGIGIGSLVGEVTGPGGVTVSYTAFVAPALMAASAMNGTVLETTFNIFFRL
                     KFDKVYDGILTTPMGPRDIAIGEIGWALLRGALYATAFLIVMVVMGLTASWWAILTIP
                     GAVLIGFAFGSVGLAATTYMRSWQDFDLVSLVTMPLFLFSATFYPLDVYPPFIQQIAR
                     LSPLYHGTEMLRAFSLGIFDWSLAGHIAFLLVMGLIGARIAARRLDGLLRK"
     gene            1576..3231
                     /gene="hutU"
                     /locus_tag="VFU96_07380"
     CDS             1576..3231
                     /gene="hutU"
                     /locus_tag="VFU96_07380"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020490248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEU5113125.1"
                     /translation="MLDLDFPLHSPHGPRLHTKGWLQEAALRCLLNNLDPEVAEDPEH
                     LIVYGGRGKAARDLASLHAIVRTLERLDDDETLLVQSGKPVGVFRTHEMAPRVLIANS
                     LLVPRWATLEHFWELEAAGLTMYGQMTAGSWIYIGTQGILQGTFQTFAAIAEQKFEGT
                     LAGKLTLTAGLGGMGGAQPLAITMNGGVALCVEVDPHRIERRLETGYLDESFDDLDAA
                     VGRALEAKRNREAVSIGLLGNAADVFPRLLEMGMDVDIVTDQTSAHDPLYGYIPTGHD
                     VDDASRLRDDDPDTYVKAARESMAAHCEAMVGFQRSGVETFDYGNNLRGEAATAGYTD
                     AFSYPGFVPAYIRPLFCEGSGPFRWVALSGDPADIAVTDRAIAELFPENESLHRWLGL
                     ASEKVHFQGLPARICWLSYGERHLAGLLFNDLVAAGSVSAPIVIGRDHLDSGSVASPY
                     RETEAMADGSDAIADWPILNAMLNVSSGAAWVAVHHGGGVGIGNSIHAGAQVVADGTE
                     QGALRVERVLTNDPGTGVMRHADAGYDIARSVARNRGVDIPML"
     gene            3336..>4116
                     /locus_tag="VFU96_07385"
     CDS             3336..>4116
                     /locus_tag="VFU96_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEU5113126.1"
                     /translation="MPINDPHWPRADVWLAQDHPDPDILVVGVPSSRASLSPSRADLS
                     PLEVRDRLSRFSTFHGEWGIDFGGVTVRDEGNWPVTDLDMHRMPDVVEELAGKLARGS
                     LTIYLGGDNAITRPLVRSLSPDLDRIGLITFDAHHDVRTLDLGPTNGTPVRGLIEGDG
                     LPGANVAQVGIHSFANSSEYRAYCDEQGITVLTVDDIAQRGIGSVVEDALTVVAVKTD
                     VVHVDVDIDVLDRAFAPGCPGARPGGMALRDLAEGVRRCARN"
BASE COUNT          613 a         1417 c         1286 g          800 t
ORIGIN      
        1 gcgatcggat atcggcgggt ttggcgcggg acggtcatct ctagcttcgt gaatcctgtt
       61 ctgttcctga cggccatggg cctgggactg ggcactctcg tcgactcctc cagcgacgac
      121 cttggtgtgc cctacctcac cttcgtcgcg accggcctca tggcggccac agccatgcag
      181 acctcggcgg gggaaggctc cttcccggtc atggccggga tcaagtggcg caagggcttt
      241 cacggcgcca tcaccacacc tctctccccc accgacatcg tgagggggat gatgatctgg
      301 gggtcgatcc ggctgacctt cgtcctcacg gtcttcgccc tggtgtcggt cgcattcggg
      361 gcgctcgacc tgggacccgc cctcctcgcg gttccaccgg caatcctcac cgggctcgcc
      421 ttccaggcct cggtcactgc attcacggtc acccgtgaga acgagatcag cctgtccacg
      481 ttgttccgct tcgggatcat ccctctgttt ctcttctccg ggaccttctt cccgatcagc
      541 cagctccccg agcagctgca ggtcgtcgcc tacttcaccc cgctctttca cggcgtggag
      601 ctggtgcgca aggtcgccct ccctggtgcc gacgcctcga tcgtcacctc tctgccgatc
      661 tggatcagtg tcgtctacct ggtggccatg gccgccgccg gcatcgtcct cggctcgcgc
      721 tggctcgacc ggagactcaa gccatgagtg tctcggctcc gagcaggctc accccgcccg
      781 gccggatgac accgttccgc gcacaacgaa tctgggagcg gaactacctt gtctaccgcc
      841 gggtgtggaa ggtggtgatc accggcttct tcgagcccgt cttctatctc ttctcgatcg
      901 ggatcggcat cggatccctc gtcggtgagg tgacgggacc cggtggcgtc acggtgagct
      961 acacggcctt cgtcgccccg gccctcatgg cggcctcggc gatgaacggg acggttctgg
     1021 agaccacgtt caacatcttc ttccggctca agttcgacaa ggtctacgac ggcatcctca
     1081 ccaccccgat ggggccgcgc gacatcgcaa tcggggagat cgggtgggcg ttgctccggg
     1141 gtgcgttgta cgccactgct ttcctgatcg tcatggtggt catggggttg accgccagct
     1201 ggtgggccat cctcaccatc ccgggtgcgg tcctcatcgg attcgccttc gggtcggtcg
     1261 gtctcgccgc gaccacgtac atgcgctctt ggcaggactt cgacctcgtc tccctggtga
     1321 cgatgcccct cttcctcttc tctgcgacct tctacccgct cgatgtctac ccgcccttca
     1381 tccagcagat tgcgcggctg tcgcctctgt atcacggcac cgagatgctc cgcgccttct
     1441 cgctcggcat cttcgattgg tccctggccg gccacatcgc cttcctgttg gtgatggggc
     1501 tcatcggcgc ccgcatcgcc gcccggcgtc tcgacggcct gctccggaaa taggcccgga
     1561 taggctcgaa tctcgatgct cgacctcgac ttccctctcc attccccgca cgggcctcgg
     1621 ctccacacca aaggctggct ccaggaggcc gcccttcgct gtctcctcaa caatctcgac
     1681 cctgaggtcg ccgaggaccc cgagcatctg atcgtgtacg gaggccgggg gaaggcagcc
     1741 cgggatctcg cgtccttgca tgccatcgtc cggacgctcg agcgcctcga cgacgacgag
     1801 accctcctgg tgcagtcggg caagccggtc ggggtgttcc gcacccacga gatggcgccg
     1861 cgtgtcctga tcgccaacag cctcctcgtg ccgcggtggg ccaccctgga gcatttctgg
     1921 gagctcgagg ctgctggtct gaccatgtac gggcagatga ccgccgggtc ctggatctac
     1981 atcgggactc aggggatcct ccagggcacc tttcagacat tcgcggcgat cgccgagcag
     2041 aagttcgagg ggacactcgc cgggaagctg accctcaccg ccgggctcgg cggcatgggg
     2101 ggcgcccagc cgctggcgat cacgatgaac gggggtgtcg ccctctgcgt cgaggtggac
     2161 ccgcaccgga tcgagcgacg tctggagacc gggtacctcg acgagagctt cgacgacctc
     2221 gacgcggcgg tggggcgcgc gctcgaggcg aagcggaatc gggaggcagt gtccatcggc
     2281 ctgctcggca acgcggccga cgtgttcccc cgattgctcg agatgggcat ggacgtcgac
     2341 atcgtcaccg accagacctc ggctcacgat ccgttgtacg gatacatccc cactggtcat
     2401 gacgtcgatg acgccagccg gctccgcgat gacgaccccg acacctacgt caaggcggcg
     2461 cgggagtcga tggctgccca ctgtgaggcc atggtcggct tccagcgctc cggggtggag
     2521 accttcgact atggcaacaa tctgcgaggt gaggccgcga ccgccggcta caccgacgcc
     2581 ttctcctatc cagggttcgt ccccgcttat atccggccgt tgttctgtga gggctccggc
     2641 ccgttccgct gggtggccct gagcggcgat ccagccgaca tcgcggtcac cgaccgggcc
     2701 atcgccgagc tgtttcccga gaacgagtct ttgcaccgct ggttgggtct ggcttcggag
     2761 aaggtccatt tccagggtct gcccgcccgg atctgctggc tctcctacgg cgagcgccac
     2821 ctggccggtc tcttattcaa cgatctcgtc gcggcgggct ccgtctcggc gccgatcgtc
     2881 atcggccgcg atcatctcga ctcgggatct gtcgcctcgc cctatcggga aacagaagcg
     2941 atggccgacg ggtcggatgc gatcgccgac tggccgatcc tcaatgccat gctcaacgtc
     3001 tcctcgggag cggcttgggt ggctgtccat catggaggcg gcgtcggaat cggcaactcg
     3061 atacatgccg gcgcccaagt ggtcgcggac ggcactgagc agggcgcgct gcgggtggag
     3121 cgggtcctca ccaacgaccc gggcaccggg gtcatgcgcc atgccgacgc cggctacgac
     3181 attgcccgca gtgtcgcccg gaaccgcggc gtcgatatcc ccatgctgtg acacccgatt
     3241 cctgaggcaa gacgtcacgc atccgtgacg gcgaccatcc gttggcgccg ggtcggttac
     3301 gggtggtcgc gcgagcggct atacgctgac cctcaatgcc gatcaatgac ccgcactggc
     3361 cacgcgccga tgtctggctc gcccaggatc acccggaccc cgacatcctg gtggtcggcg
     3421 tcccgtcgtc tcgggcatct ttgtcgccat cgcgagccga cctgagcccg ctcgaggtcc
     3481 gcgatcgtct gagccgtttc tctaccttcc atggggagtg gggcatcgat ttcggtggtg
     3541 tcacggtgcg tgatgagggc aactggccgg tcacagatct cgatatgcat cggatgcccg
     3601 acgtcgtcga ggagctggcc ggcaagctcg ccagaggctc gctgaccatc tatctcggtg
     3661 gagacaacgc catcacccga cccctggtcc gatcgctgtc gcccgacctg gatcggatcg
     3721 ggctcatcac cttcgacgcc caccacgatg tccggaccct cgatctaggc ccgacgaacg
     3781 gcactccggt gcgaggcctc atcgaaggcg acgggctgcc aggtgcgaat gtggcccagg
     3841 tcggaatcca ctcctttgcc aactcctccg aatatcgggc ctattgcgac gagcagggca
     3901 taacggtcct gaccgtcgac gacatcgcac agcgcggcat cgggtctgtg gttgaggatg
     3961 ccctcacagt cgtcgctgtg aaaaccgacg tcgtccacgt ggatgtggac atcgacgtcc
     4021 tcgatcgtgc cttcgcaccc ggttgcccgg gggcgagacc gggggggatg gccctgcgcg
     4081 atctggcgga aggtgttcgt cgttgtgccc gcaacc
//
LOCUS       DASTXO010000350         4323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_57831, whole genome shotgun sequence.
ACCESSION   DASTXO010000350 DASTXO010000000
VERSION     DASTXO010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57831"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="VFU96_07390"
     CDS             <1..945
                     /locus_tag="VFU96_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEU5113127.1"
                     /translation="QVVTTVLVSEYLPDAYVEMLRARHEAIYDPDLCTDRSRLLDQMG
                     GVEAIFIRNRTRIDDELIAAGADLRVVGRLGVGLDNIDLSACERAGVRVFSAVGANAV
                     SVAEYVIGAMLVLVRGVFGMSPSMVAGEWPRQGHAFGFELMGKAIGLVGFGSIAREVA
                     TRAAALGMDVLAHDPFLPDDDPAWAIVRSVDMDTLLASADVISLHVPLTDETRNLIDA
                     RVLGRMRPTAVLINTARGGIVDESALAEALREGRLGGAVLDVFAAEPLGPEPAAVFAG
                     IPNLLLTPHVAGNTRESVDRVARMIVEKVLEELGPNSD"
     gene            complement(942..2198)
                     /locus_tag="VFU96_07395"
     CDS             complement(942..2198)
                     /locus_tag="VFU96_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEU5113128.1"
                     /translation="MIRDLVADQEGAISRYAKGPFAVDWEDRYDFAELRRQRVSKAHE
                     ALAGSDADAVLVWKDENVRYLTSLRAQLIAGKTTSLNGALLTADDDPVLLGSGGEIDK
                     ARFGMSWIGEAHAVPIMEQRELVDGFVTDILIPLLQTRGLTTGRLAVDQANISFIDSM
                     RAHLPDLELVDGDTVMQQARWIKTDLEVAVIEEACAIGDSVTQRALDETRAGRRELDV
                     AADAMQTLFQLGGEMAHVITPFVATGEHMSPPHRLATDKIIRNQDLCFIDIGAMWNGY
                     FADIGRTTIVGKPSREQQDIYTAVYEGLMAGLDSMRPGNTNQDCADAIIGKIAEHGLE
                     DHLFSLFIGHGIGMGANEPPYIGETLPGATTYDFEPNMVFAVEPLVWVPNVRGGGGVR
                     LEDMILITEGDPHLLSRVEYEERLLV"
     gene            2227..2901
                     /locus_tag="VFU96_07400"
     CDS             2227..2901
                     /locus_tag="VFU96_07400"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU5113129.1"
                     /translation="MEDRLRLDVSRSRRVTAVDLVRDALRTAILRGDLPGGSRLVQTD
                     IATQLEVSTTPVREAMRDLASEGLITLDSHRIGTVRMADWDEMVEIVEMRRSLEGLAI
                     HRAMAQITPEELKEAEQVATQLSEEEDLGSWVQTNILFHSIFHKATRSRRLSGVLLAL
                     EEAGGVFVAQAQQLHPEIRRRAIADHFALLDAFAAREVDRAIEIQHGHINLPLEAFEL
                     DGGRPS"
     gene            complement(2906..3679)
                     /locus_tag="VFU96_07405"
     CDS             complement(2906..3679)
                     /locus_tag="VFU96_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEU5113130.1"
                     /translation="MPDLMKHRLQRGERLVVFAVGRMFHPNMIRYLGMAGDFDGFWID
                     VEHGGLTTHDIEIAATAGLAHGLDCFVRVPPTDYATVTRCFESGATGVMAAQIKSAEE
                     AEEFVRWAKFAPRGRRGLNPLGYDGRYGTIPLAEFAEQANRDTFVAVQIETAEAVEEV
                     DEIAAIDGVDLLFVGPSDLSQAVGVIGDFMGDASLAAVDRVAAACRAHGKQWGAVTPT
                     PDYAALLIEKGCTLISPVNDVKLVTTGLGAMKETYSLLW"
     gene            3731..3934
                     /locus_tag="VFU96_07410"
     CDS             3731..3934
                     /locus_tag="VFU96_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113131.1"
                     /translation="MPVIDMHAHVTPERYKKAIRERGEWHGLDAEAGELGLGGFDKML
                     DERLEEMDSLGVEMQLFTPTVGF"
     gene            3956..>4323
                     /locus_tag="VFU96_07415"
     CDS             3956..>4323
                     /locus_tag="VFU96_07415"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5113132.1"
                     /translation="MTRRIARECNDEIAEMVGAHPTRFAGLATLPMQDPPSAIVEMER
                     AMTGLGLLGVIVSDHVAGRTYDEPDFLPFFSAAEELGAIVFFHQGGDTVVNHRIRRYK
                     LGNAVGNLTERALVFATLVFG"
BASE COUNT          756 a         1345 c         1410 g          812 t
ORIGIN      
        1 caagtagtga ccaccgtcct ggtctctgag tacctgcccg atgcatacgt ggagatgctt
       61 cgagctcgtc atgaggccat ttacgacccc gacctctgca ccgaccgaag ccggctcctc
      121 gatcagatgg ggggcgtcga agccattttc atccgcaatc gcacccggat cgacgatgag
      181 ttgatcgctg ccggtgccga tctgagggtg gtcgggcggc tcggggtggg tctggacaac
      241 atcgatctga gcgcctgtga gcgagcaggt gtgcgcgtgt tctcggccgt cggagccaac
      301 gccgtctcgg tcgccgagta cgtcatcggg gcaatgctgg tcctggtcag aggcgtcttc
      361 gggatgtcgc catcgatggt cgccggcgag tggccccgtc aaggccacgc cttcggattc
      421 gagctgatgg gcaaggccat cggtctcgtc ggcttcggct ccatcgccag ggaggtggca
      481 acgcgcgccg ccgcgttggg aatggacgtc cttgcccacg atccgttcct gccagatgac
      541 gacccagcgt gggcaattgt tcgcagcgtc gacatggaca cattgctcgc ctcggcggac
      601 gtgatcagtc ttcatgtccc gctgacggat gagacccgca acctgatcga tgcccgcgtg
      661 ctggggcgta tgagaccgac cgctgtcctg atcaacaccg cccggggtgg catcgtcgac
      721 gagtcggctc ttgccgaggc tcttcgcgag gggaggttgg ggggtgccgt cctcgacgtg
      781 tttgccgccg agcctctagg accggagccg gcagccgttt tcgccggaat ccccaacctg
      841 ctcttgacgc ctcatgtcgc cgggaacacc cgcgagtcgg tcgaccgggt ggcgaggatg
      901 atcgtggaga aggtgctcga ggagctgggc ccgaattcgg actagacgag aaggcgctcc
      961 tcgtactcga cccgtgagag gaggtgtgga tcgccttcgg tgatgagaat catgtcctcg
     1021 agacggacgc cgccgccgcc ccgaacattc ggcacccaaa ccaacggctc gactgcgaac
     1081 accatgttgg gctcgaagtc gtaggtcgtg gccccgggga gggtctcccc gatgtagggg
     1141 ggctcgttgg cgcccatccc gatcccatgg ccgatgaaga gactgaatag atgatcctcg
     1201 agaccgtgct cggcgatctt gccgatgatc gcatcggcgc agtcctggtt ggtgtttccc
     1261 ggtcgcatcg agtcgagtcc ggccatcaac ccctcgtaaa cggcggtata gatgtcctgt
     1321 tgctcccgtg agggcttgcc cacgattgtc gtgcgcccga tgtcggcgaa gtagccgttc
     1381 cacatggcac ctatgtctat gaagcagagg tcctggttgc ggatgatctt gtcggtggcg
     1441 aggcggtggg gtggcgacat gtgctccccg gtggcaacga aaggcgtgat cacgtgagcc
     1501 atctcaccgc cgagttggaa caaggtctgc atggcgtcgg cggccacgtc gagctcgcga
     1561 cgaccggctc gggtctcatc cagggcgcgc tgggtcaccg agtcaccgat ggcgcacgcc
     1621 tcttctatca ccgccacttc gagatcggtc ttgatccatc gcgcctgctg catgacggtg
     1681 tccccgtcga cgagctccag gtcggggagg tgagcacgca tcgagtcgat gaaggagatg
     1741 ttggcctggt ccaccgcgag tcttcccgtg gtcaagcctc tcgtttggag aagggggatc
     1801 aggatgtcgg tgacgaaacc atcgaccagc tctctctgct ccatgatggg gacggcatgg
     1861 gcttcaccga tccagctcat cccgaaccgg gctttgtcga tctcgccacc cgatccgagc
     1921 aagaccgggt cgtcgtcggc ggtgagaagc gctccattca atgaggtggt cttgccggcg
     1981 atgagctggg cccgcaacga ggtgagatag cggacgttct cgtccttcca gaccaacacg
     2041 gcatcggcat ccgaaccggc aagggcctca tgagccttgc tcacacgctg ccgacgcagc
     2101 tcggcgaagt cgtaccggtc ctcccagtcg acggcgaaag gccctttggc gtatctggag
     2161 atggctccct cctggtctgc gacgaggtcg cggatcatac gctttatatt cggtagagta
     2221 cagaaagtgg aggaccgact ccgcctcgat gtttcgcgct cccgacgagt caccgccgtc
     2281 gacctggttc gggacgcctt gcgcaccgcg atcttgcggg gtgacctccc cggagggtcg
     2341 cgtttggtgc agaccgacat cgccacccag ctcgaagtga gcactacccc ggttcgggag
     2401 gcgatgcggg atctggcgag tgaggggctg atcaccctcg acagtcaccg aatcggcacg
     2461 gttcgcatgg cggattggga tgagatggtg gagatcgtcg agatgcgccg gtctttggag
     2521 ggccttgcca tccaccgggc gatggcgcag atcacccctg aggagctgaa ggaggccgaa
     2581 caggtggcga cccagctctc ggaggaggag gatctgggtt cctgggtgca gacgaacatc
     2641 ctctttcatt cgatctttca caaggcgacg cgaagtcggc gcctgagtgg agtcctgctt
     2701 gctctggagg aggcaggcgg cgtgttcgtg gcccaggcgc agcaacttca cccggagatc
     2761 cgacgaaggg ccattgcgga tcatttcgcc ctgctcgacg ccttcgccgc gcgagaggtc
     2821 gatcgggcca tcgagatcca gcacggacac atcaacctgc ctctggaggc tttcgagctc
     2881 gacggcggcc gcccgagctg ataactcacc agagcaggga gtaagtctcc ttcatggccc
     2941 cgagacccgt ggtcaccaat ttcacgtcat tcaccggcga gatcagggtg catcccttct
     3001 cgatgagcag ggcggcgtag tcgggggtgg gcgtcaccgc cccccactgt ttgccatgcg
     3061 cccggcaggc cgcggccacc cggtccaccg ccgcaagcga ggcatcgccc atgaagtcgc
     3121 cgatcacgcc cactgcctga ctcaggtcgg aaggcccgac gaacaagagg tccacgccgt
     3181 cgatggccgc tatctcgtcg acctcctcga cggcctcggc cgtctcgatc tgcacggcca
     3241 cgaaggtgtc acggttcgcc tgttcggcga actcggccag cgggatcgtc ccgtagcggc
     3301 cgtcgtaacc gagcgggttc agcccgcgcc ggccccgcgg ggcgaacttg gcccatcgga
     3361 cgaattcctc ggcttcctcc gcgctcttga tctgggcggc catcaccccg gtggcaccac
     3421 tctcgaagca ccgggtgacc gtggcgtaat cggtgggagg gacccggacg aagcaatcga
     3481 gaccatgggc caaccccgcc gtcgccgcga tctcgatgtc atgtgtggtg agaccaccgt
     3541 gctcgacgtc gatccagaag ccgtcgaaat caccggccat tccgaggtag cggatcatgt
     3601 tgggatggaa catcctcccg acggcaaaaa ccaccagtcg ctcccctcgc tggagacggt
     3661 gtttcatcag gtcgggcatc ggtttttccc cagtcggttc ggtagaatgc ataatgtaat
     3721 atcgatccac atgcccgtga tcgacatgca cgcccacgtc accccggagc ggtacaagaa
     3781 ggcgatcagg gaacggggcg aatggcacgg tctcgacgca gaagccggtg agctcggcct
     3841 gggcggcttc gacaagatgc tggatgagcg tctcgaggag atggattcgc tcggtgtcga
     3901 gatgcagctc ttcacgccca ccgtcggctt ctaacagtac gggaccgagc tcgacgtgac
     3961 acggaggatc gcccgggaat gcaacgacga gatcgccgag atggtggggg ctcacccgac
     4021 caggttcgcc gggttggcca cgttgccgat gcaggacccc ccgagtgcca tcgtggagat
     4081 ggagcgggcc atgactggtc tcggcctcct cggcgtgatc gtcagtgatc acgtcgccgg
     4141 tcggacctac gacgagccgg atttcctccc cttcttcagc gcggccgagg agcttggagc
     4201 catcgtgttc ttccatcagg gcggtgacac ggtggtcaac caccgcatcc ggcgctacaa
     4261 gttgggcaat gccgtgggca atctcaccga gcgagccctg gtctttgcga cgctcgtctt
     4321 tgg
//
LOCUS       DASTXO010000351         2626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_500411, whole genome shotgun sequence.
ACCESSION   DASTXO010000351 DASTXO010000000
VERSION     DASTXO010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500411"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VFU96_07420"
     CDS             <1..515
                     /locus_tag="VFU96_07420"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEU5113133.1"
                     /translation="RAGLDRLGRLLKVSGPEVPGRVEGLLERIDTLEGELDALRSQRR
                     GELAIELAGQAKEIGGTSLLVGSAPDLDANSLRQVALGIRDRLGSPSVAVVGSIVAGK
                     GSLIAVVTKDLVGAGVSAAEIISDAATELGGGGSRDPELAQAGGPHGDRLDDALDQAR
                     DTAERLLGSL"
     gene            512..916
                     /gene="ruvX"
                     /locus_tag="VFU96_07425"
     CDS             512..916
                     /gene="ruvX"
                     /locus_tag="VFU96_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEU5113134.1"
                     /translation="MSRALGVDYGTKRVGLAISDSLGITARPLSVVPRSVVVEEVVNL
                     VKEHDVGTIVVGLPTGLSGDEGVSASEARKLADELGVAAGVDVVLVDERYTSRLAEDA
                     LLESGMKRRKRRESVDKVAAAIILQDYLDRTK"
     gene            913..2016
                     /gene="mltG"
                     /locus_tag="VFU96_07430"
     CDS             913..2016
                     /gene="mltG"
                     /locus_tag="VFU96_07430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU5113135.1"
                     /translation="MTYEPAPEQPSPWIRASKIAAIIGLVVLAGIAVVGGGSYLGRLV
                     GSSFNSDDPSASPVSVQPGQDVTIEIPSGSTAEDIGAILAAQGVVRSALEFEVAVRNN
                     EADARLQAGTYAMQTVMDPNEVVAMLVVGPVADVYRATVIEGLKVDEILTALAEASGI
                     PYSDFETALLSGEVDTALAQIPDDPALADWEGLLFPDTYEFASSADPVHILQRLASTM
                     EQRVGSVDWATLEAAGMTPYDGLKIASLIESEAQLDEERPTVSSVIHNRLNMGMKLDI
                     DATVLYALGTDDPALFDREVDSPYNTYRVNGLPPTPISSPGRASLEAAAAPEQTDFLY
                     YVLSDLDGHHAFAATLDEHNANVAQAREDGVLP"
     gene            2047..>2626
                     /gene="aroE"
                     /locus_tag="VFU96_07435"
     CDS             2047..>2626
                     /gene="aroE"
                     /locus_tag="VFU96_07435"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="AroE; catalyzes the conversion of shikimate to
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEU5113136.1"
                     /translation="MPSNLRLAVLGDPIEHSRSPAIHNAAMAHLGIDGSYVARRVEAG
                     SLGGVIEELRNGSLTGVNVTMPLKTEAAALADVLTEEASASESVNTIRMRSGLVEGHS
                     TDVVASARAYSDDRFDPSVPILVLGAGGAAAAALIAGAGRDLYIAARDIQQASALVAR
                     IDDGAAVVPFGVGVSRALLVNATPLGKDGTSLP"
BASE COUNT          457 a          847 c          868 g          454 t
ORIGIN      
        1 tgcgggccgg gctcgatcgc ttgggccggt tgctcaaagt ctctggcccg gaggtcccgg
       61 ggcgcgtaga gggcctcctc gagcggatcg acacgctgga aggcgagctc gacgcactgc
      121 gctcccagcg acgaggagag ctggccatcg aactcgccgg gcaggccaaa gaaatcggtg
      181 ggacgtcatt gctcgtgggc tccgcccccg acctcgacgc caactcactg cgccaggtgg
      241 cgctggggat tcgggaccgc ctcggatctc cgtctgtggc cgttgtggga tcgatcgtgg
      301 caggcaaagg ctcattgata gccgtggtta cgaaggacct ggtgggggcc ggtgtttctg
      361 ccgccgagat catctccgat gccgcgaccg agctcggggg cggcggatcc cgcgatccgg
      421 agttggccca ggccggtggt ccgcacgggg accgcctcga cgacgcgctc gatcaggccc
      481 gggacaccgc cgaacgtctt ctcgggagtc tgtgagccgg gccctcggtg tcgattacgg
      541 gacgaagcgg gtcggtttgg cgatatccga ttccctcggg atcaccgccc ggccgctatc
      601 cgtcgtgccg aggtcagtgg ttgtggagga ggtggtgaac ctcgtcaaag aacacgatgt
      661 cggtaccatt gtcgtcggcc tccccaccgg gctgagtggt gatgaagggg tgtccgcatc
      721 cgaagccagg aaacttgcgg atgagctcgg cgtcgcagcc ggggtcgatg tcgtcctggt
      781 tgacgagcgt tacacctcga gattggccga agacgcgctc ctcgaatcgg gcatgaaacg
      841 ccggaagcga cgagagagcg tggacaaggt ggcagccgca atcatccttc aggactatct
      901 ggaccgaacc aaatgacata cgagcctgcg cccgagcaac cctccccgtg gatccgggcg
      961 agcaagatcg cagccatcat cgggctggtc gtgctcgccg ggatcgcggt agtgggcgga
     1021 ggctcctacc tgggacgtct cgtcgggtct tctttcaact ccgacgaccc ttcggcgagc
     1081 ccggtgtctg tccagcccgg gcaggacgtg acgatcgaaa tcccatcggg gtcgacggcc
     1141 gaggacattg gagccatcct cgccgcccaa ggagtcgttc gctcggcact cgaattcgag
     1201 gtggcggtcc gcaacaacga ggcagacgca cgtctccagg ccggtactta cgcgatgcag
     1261 accgtgatgg atcccaacga ggtggtggcc atgctcgttg tcggtccggt ggcggacgtc
     1321 tatcgggcga cggtgatcga gggcctgaag gtagatgaga tcctcaccgc cctcgccgag
     1381 gcatcaggga ttccctattc cgacttcgaa actgcgctgc tcagcggtga agtcgacacg
     1441 gccttggctc agattcccga tgacccggcg ctggcagact gggaaggctt gcttttcccc
     1501 gatacgtacg agttcgccag ctcggccgac ccggtgcaca tcctgcaacg cctcgcatcc
     1561 accatggagc agcgggtggg aagcgtcgac tgggccacct tggaggcggc cgggatgacc
     1621 ccatacgatg ggttgaagat cgcctcgctg atcgagtccg aggcgcagct ggacgaagag
     1681 cgcccgacgg tctccagcgt gatccacaac cgcctgaaca tgggcatgaa gctcgatatc
     1741 gacgccacgg ttctctacgc cctcggcacc gacgatcccg cgctcttcga tcgggaagtg
     1801 gactcgccct acaacaccta tcgggtcaat gggttgcctc cgactccgat ctcctccccg
     1861 ggccgagcct cgctggaggc ggcagctgcg ccggaacaga ccgacttcct gtattacgtg
     1921 ctgtccgacc tcgacggcca tcacgccttc gcagccactc tcgatgagca caacgccaac
     1981 gtggcccagg cccgggagga tggcgtcctt ccctaggcgc tgactaccgc tgcaacactg
     2041 gcggccatgc cttccaacct tcgtctggcg gtgctcggcg atcccatcga gcactctcga
     2101 tcgcccgcca tccacaacgc tgccatggcg catctcggga tcgatggctc gtatgtggcg
     2161 agacgagtcg aagcagggtc actgggcggc gtcatcgagg agctcaggaa cggctcgctc
     2221 acgggcgtaa acgtgaccat gccactgaag accgaggcgg ccgcactcgc agatgtccta
     2281 accgaggaag cgtcggccag cgagtcggtc aacaccatcc gcatgcgatc aggcctggtc
     2341 gaaggtcaca gcaccgatgt cgtcgcatcg gcacgggcct actcagacga ccggttcgac
     2401 ccgtcggtcc cgatcctggt tctcggagct ggtggggcag cggcggcggc gctcatcgcc
     2461 ggcgccgggc gcgatctgta catcgccgca cgcgatattc aacaggcctc cgccctcgtc
     2521 gcgaggatcg acgatggggc agcggtggtc ccgtttggtg tcggggtcag ccgcgccttg
     2581 ctcgtgaatg ccacgccact cggcaaggac ggaacgtccc tgcccg
//
LOCUS       DASTXO010000352         5373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_95222, whole genome shotgun sequence.
ACCESSION   DASTXO010000352 DASTXO010000000
VERSION     DASTXO010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95222"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VFU96_07440"
     CDS             complement(<1..527)
                     /locus_tag="VFU96_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113137.1"
                     /translation="MPYSLMGLAALVGSFFYSEMPDWGYWLPFAALFVLLEFFAVEVN
                     DRMLQSSSLMVAMTAGVILAMAPDSDAMFGLALMAALGPFTVVDFREKRWFQPLANFG
                     QMVTAGAVAGFILDLFLEGVEASTNTLILVLIVSAAAALAYTTVQTVLVRHAVKVVFG
                     RDNLEPWSQMPVLFTG"
     gene            1083..1703
                     /locus_tag="VFU96_07445"
     CDS             1083..1703
                     /locus_tag="VFU96_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113138.1"
                     /translation="MDLIALQDKICRIEGVEAARIVAGNGHIEEIHVLARRNKPPKQV
                     VRDVQSLSQALFGEEIDRRIVSVVQLADADLDMGFRPALVDVSEILDGSRAQVSVTLR
                     WQNEVFTGTSKGAAAMTTRSRQIAEATLEAIRQAIHQEAALAVSSMDIPVLGSRKVAV
                     AQVVVVTEASERHLIGCAYVEDEESRAVVRSVLDALNRVLPDLKTS"
     gene            1754..3244
                     /locus_tag="VFU96_07450"
     CDS             1754..3244
                     /locus_tag="VFU96_07450"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEU5113139.1"
                     /translation="MLVGGGNAVDAAIAATATQGVVAPETCGIGGDLFALVHRPGSEQ
                     PRALNASGRAGSNANPEPHRGLESIPRDHPLSVTVPGCVDGWSALSAELGRLPLKECL
                     SPAIEHATEGFTVSTEQAVACAKEASVYAGHPAIREFYPEGRPVTRGDLVRRPALAAT
                     LTSIAESGREAFYLGQPAQDIVEELGGVITADDLARPHADWVRPISCPVGGFTAWTIP
                     PNSQGYLGPATLAVFEMLDPPRDPAEPGWWHLLIEAFRSVAWERDDLVADPDHVPLPA
                     DLLLDSERLARAAATVDKERTGTWPRRMGAASSTAFLCVVDAEGMAVSIIQSNYHGTG
                     SHFGAGRSGFLLHNRGSGFTLTPGHPNELLPGKRPLHTLSPTLWTSGTSTRWVLGTRG
                     ASLQPQILGQVGARAILAGQTLEVAQDAPRWAISEFGPFSDARFQVEPGVPAGTLSEL
                     RRRGHIIEEVAGRQPGWGPVSIIEAQKDHRSTHRDPRVDTTSAVVF"
     gene            complement(3248..4261)
                     /locus_tag="VFU96_07455"
     CDS             complement(3248..4261)
                     /locus_tag="VFU96_07455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEU5113140.1"
                     /translation="MKVDFYYPPSPPEGAPEAAMAAVDLGYDGFFTAETQFDPFLPLA
                     LAAGAQPELELGTAIAVAFPRSPMITAMTGWDLARMSRGRFMLGLGTQVRAHIVRRFS
                     TTWDRPVPRLRDYILALRAIWESFQNSSPLAFEGDFYQFSLMTPFFNPGPIPHPDVPI
                     YIAGVGPYLSRLAGELCDGFHVHPFHTVRYLDEVVLPGIAAGSAGAGRTIDDVELATT
                     VFVMTGATESEVEQAMGPVRQQIAFYASTPSYLPVLEAEGWDFGGQLNAMSKQGRWEE
                     MARLVPDEAVLTVGVAATSDRLAEVIQDRYRDRVQRLGFYTIGPTLDTDPEGLREVIR
                     RLK"
     gene            4309..4629
                     /locus_tag="VFU96_07460"
     CDS             4309..4629
                     /locus_tag="VFU96_07460"
                     /inference="COORDINATES: protein motif:HMM:NF025873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEU5113141.1"
                     /translation="MKTLAKVVGIVGAAAALVWAMRDRFISVAASREPEPPVLRTATP
                     VATAEGIDAIDGIGPVFASRLKDAGIDDVSKLASATPDNIAEAAGVSGARARTWIEQA
                     RSRG"
     gene            4632..>5373
                     /gene="rlmN"
                     /locus_tag="VFU96_07465"
     CDS             4632..>5373
                     /gene="rlmN"
                     /locus_tag="VFU96_07465"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine(37)-C2)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine(2503)-C2-)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEU5113142.1"
                     /translation="MPYLHRPEDLGDVLEGQPSYRVDQLRNWLYRTPVLSSTDMTNLP
                     ADVREKLEPLWPFEVEAEQSADGATTRKWLFRAPDGAAIEAVLMGYPRRATLCISSQA
                     GCALGCTFCATGQFGFERHLDAGEITAQVAYAQAFLRQIGMPGTPGHLTNVVFMGMGE
                     PLANYDRVREAIRRMIEIMGLSARSITVSTVGIVPGIRRLAEEPWPVSLAVSLHAADD
                     ELRSTLVPINRRYPLAEVEEAAGLYFEKK"
BASE COUNT         1010 a         1730 c         1726 g          907 t
ORIGIN      
        1 cccgtgaaca gaactggcat ctgcgaccag ggctccaggt tgtcacggcc gaagacgacc
       61 ttcaccgcgt gccgcacgag gacggtttgc acggtggtat acgccaaagc cgcggctgcc
      121 gacacgatga gcaccaggat caacgtgttc gtggatgcct cgacaccttc gagaaacaaa
      181 tcgaggatga agccggcgac ggcgccggca gtcaccatct ggccgaagtt ggcgagaggt
      241 tggaaccatc gcttctccct gaaatcgacc accgtgaatg ggccaagggc cgccatcagg
      301 gcgaggccaa acatggcatc ggaatcgggt gccatggcga ggatcacccc ggcggtcatc
      361 gccaccatca gcgaagagga ttggagcatg cggtcgttga cctcgaccgc gaagaactcg
      421 aggaggacga agagggcggc aaatgggagc caatagcccc agtccggcat ctcggagtag
      481 aagaacgaac cgacgagggc ggccagaccc atgagcgagt aaggcacagc gatcctcaat
      541 cgacgctgca ccgtggggcg gagacgacca aaccatcccc gcaacggtct cttcctacct
      601 agcctcattg agcccctcga cgacatgtat gaccgtggtt tggtgagccg gcgcctgtta
      661 tcagccgatt tttatgccgg cgagcactaa ctccaggctc gcgacacatc tgtgattgcg
      721 ggccagaaat gccttaatca aggaacgctc acctgttggt gccgtatttc accgacgttg
      781 tatgccggtg ttgacgatcc gctccgctgg gggtcgatgg gcgtccagtt acaaggccga
      841 cgtctgacgc cggtctcacc gctttccccg gcaagtcttg acggggaacc tttaacatgt
      901 caaaggcccc tgacttcgcc cgccataggc caggcagacg ggtcagagac ctcggctgcc
      961 ccacgtttcc aataactgcc ttccagcaag gcgcaatcta gagggttcgc gaactgtgga
     1021 caagaggaaa tggggtcggt tatcccaata gaccttcctc gttgcctgta cgctgagtag
     1081 atatggacct aatcgcgctc caggacaaga tctgtcgcat cgagggtgtc gaagcggcga
     1141 ggatcgtcgc cggcaacggc cacatcgagg agattcatgt cctggcccgt cgcaacaagc
     1201 ctcccaagca ggtcgtgaga gacgttcagt ccctcagcca ggcgctcttt ggcgaggaga
     1261 tcgaccgccg gatcgtgtcc gtggtccagc tcgccgacgc cgatctcgac atggggttcc
     1321 gcccggctct cgtcgatgtg agcgagatcc tcgacggatc ccgcgcccag gtctcggtga
     1381 cactgcgatg gcagaacgag gtcttcaccg gcacttcaaa aggcgcagca gccatgacga
     1441 cccgatcccg acagatcgct gaagcgaccc tcgaggccat tcgccaggca atccatcagg
     1501 aggccgcgct tgcggtctcg tcgatggaca tacctgtgct gggaagccga aaggtggcag
     1561 tggcccaggt tgtcgtggtc accgaggcat ccgagcgaca cctgatcggg tgcgcctacg
     1621 tcgaggacga ggagtcgaga gcggtggttc gctccgttct cgatgccctc aatcgggtgc
     1681 tccccgatct caagacatcc tgacgcacgc ggtctccacc ccccatcatc tctccaccga
     1741 cgccgccagg cgtgtgctgg ttggaggagg caacgcagtg gacgcggcaa tcgcggccac
     1801 tgcaacccag ggcgtcgttg ccccagagac atgcgggatc ggcggcgatc tcttcgcctt
     1861 ggtgcaccgg ccgggatcgg agcagccgcg ggcactcaat gcttccggcc gggccggttc
     1921 caacgccaat ccagaacctc atcgtggcct cgaatccatt cccagagacc acccactgtc
     1981 tgtcaccgtc ccgggttgtg tcgacggctg gtcggccctc tccgccgagc tggggcgcct
     2041 gccgctgaag gagtgcctat caccggcgat cgagcatgcc accgagggat tcaccgtgtc
     2101 caccgagcag gccgtcgcct gcgccaagga ggcaagcgtc tacgccgggc accctgcgat
     2161 ccgcgagttc tatccggagg ggaggccggt gaccagagga gatctggtca gacgccccgc
     2221 attggcggcg accctcacgt cgatcgccga atcgggccgt gaggccttct atctcgggca
     2281 gccggcgcag gacatagtgg aggagctggg tggtgtgatc acggcagatg atctggcccg
     2341 gccccatgcc gattgggtga ggccgatctc gtgtccggtc ggcggcttca ccgcctggac
     2401 gatcccaccc aattcacagg ggtacctcgg tcccgccacg ctcgcggtgt tcgagatgct
     2461 ggacccgccc cgtgatccag ccgagccggg ctggtggcac ctgctcatcg aggcgtttcg
     2521 ctcggtggcc tgggagcggg acgacctcgt ggcggatccc gatcatgtcc ccctccccgc
     2581 tgatctgcta ctcgatagcg aacggctggc tcgcgccgcg gcaaccgtgg acaaagaaag
     2641 gacaggaacc tggcctcgga ggatgggggc ggcttcgagc acggcatttc tctgcgtcgt
     2701 tgatgctgag gggatggccg tatcgatcat ccagtcgaac tatcacggga ccggctccca
     2761 cttcggggcg ggccgcagcg ggttcctcct ccacaatcgg ggcagcggct tcaccctgac
     2821 tccgggacat cccaacgagc tcctgcccgg caagcggccg ctccacaccc tgtcccctac
     2881 cttgtggacc tccggcacat cgacacgctg ggtgttggga acccggggcg cctcgttgca
     2941 accacagata ctgggccagg tcggggcacg agcaatcctg gccgggcaga cactcgaggt
     3001 ggcgcaagac gccccgaggt gggcgatctc ggagttcggg ccattctcgg atgcccgatt
     3061 ccaggtggag cctggcgtcc ccgccggcac cctctctgag ctccgacgcc gcggtcacat
     3121 catcgaggag gtagccggcc gccaaccggg ctggggcccg gtctcgatta tcgaggccca
     3181 aaaggaccat cgctccacac atcgggatcc gcgcgtcgac accacgagtg cagtggtctt
     3241 ctgacgtcta cttgagccga cgaatcacct cgcggagacc ctccgggtcg gtgtccaacg
     3301 tgggtccgat cgtatagaag ccgagtcgct gcactcgatc gcgatagcgg tcctgaatga
     3361 cctcggccag ccgatcggag gtagccgcca cgcccacggt gagaactgct tcgtcgggga
     3421 cgagccgagc catctcctcc catcgcccct gcttcgacat cgcgttgagc tgaccgccga
     3481 agtcccagcc ctccgcctcc agcaccggca gatatgaagg cgttgaggcg tagaacgcga
     3541 tctgttgtcg gacaggtccc atggcctgct cgacctcgga ttcggttgca ccggtcatca
     3601 cgaacacggt cgtcgccagc tcgacgtcat cgatcgtccg cccggcgcct gccgacccgg
     3661 ccgcgatccc ggggaggacc acttcatcga gatagcgcac agtgtgaaac ggatggacgt
     3721 ggaacccatc gcagagctcc ccggccagac ggctcaggta cggccccacg ccggcgatgt
     3781 atatcggcac gtctggatgc gggatgggac ccgggttgaa gaagggtgtc atcagggaga
     3841 attggtagaa atcgccttcg aaggcgagcg ggctcgagtt ctggaacgat tcccagatcg
     3901 cgcgtagggc aaggatgtaa tcccgcagac ggggcaccgg gcgatcccag gttgtcgaga
     3961 agcgccgaac gatgtgcgcc ctcacttgag tccccagccc gagcatgaac cgcccgcgcg
     4021 acatccgggc gaggtcccaa ccggtcatcg ccgtgatcat gggagacctg gggaaggcga
     4081 cggcgattgc cgtcccgagt tcgagctcgg gttgcgcgcc tgcggccagg gccagcggca
     4141 ggaaggggtc gaactgggtc tcggcggtga agaagccgtc gtagccgaga tccaccgccg
     4201 ccatcgcagc ctcgggagca ccttctggcg ggctgggcgg gtagtagaag tcgactttca
     4261 tcagccggcc agggtagatc gggggacgaa tgggtacgat caacagcgat gaaaacgctg
     4321 gccaaagtcg tagggatcgt gggcgcagca gccgccctgg tctgggcgat gcgcgaccgg
     4381 ttcatctccg tggccgcgtc ccgggaacca gagccacccg tgctacgaac cgcgaccccg
     4441 gttgcgaccg ccgaaggcat cgatgcgatc gacggaatcg gccctgtctt cgccagccgt
     4501 ctcaaagacg ccgggatcga cgatgtctcc aagctggctt cggcaacacc cgacaacatc
     4561 gccgaggcgg ccggagtcag cggcgcccgg gccaggacct ggatcgagca ggctcgctcg
     4621 cggggctgac ggtgccctac ctccaccggc cggaggatct cggcgacgtc ctcgaggggc
     4681 agccctccta tcgagtcgac caattgcgca actggctcta tcgaacgccg gtcctgagca
     4741 gcacggacat gaccaatctc ccggccgacg tgagggagaa gctggagcct ctctggccct
     4801 tcgaggtgga agcggagcaa tccgcggatg gcgctaccac tcgcaaatgg ctgtttcgcg
     4861 ctcccgacgg tgcggccatc gaggcggtgc tgatggggta tccacggcgg gcgacgcttt
     4921 gcatctccag ccaggccggt tgtgccctcg gatgcacctt ctgtgcgaca gggcagtttg
     4981 gcttcgagcg gcatctcgac gccggcgaga tcacggcgca agtggcatac gcacaagcgt
     5041 tccttcgtca gatcggcatg cccggcaccc ctggccatct gaccaatgtc gtgttcatgg
     5101 gaatgggtga gccactcgcc aactacgacc gcgtcagaga ggcgatccgg cggatgatcg
     5161 agatcatggg attgtctgcg agatcgatca ccgtgtcgac ggtcggcata gtgccgggaa
     5221 tccgtcgact cgctgaagag ccttggccgg tctctcttgc ggtcagcctt catgcagcgg
     5281 acgacgagct ccggagcacg ctcgtcccga tcaatcggcg ataccccctt gcagaggtcg
     5341 aggaggccgc cggcctgtac ttcgagaaga agc
//
LOCUS       DASTXO010000353         6451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1642326, whole genome shotgun sequence.
ACCESSION   DASTXO010000353 DASTXO010000000
VERSION     DASTXO010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6451
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642326"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /gene="selA"
                     /locus_tag="VFU96_07470"
     CDS             complement(<1..779)
                     /gene="selA"
                     /locus_tag="VFU96_07470"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEU5113143.1"
                     /translation="MASSKNPYRGLPPVDQLVDGLGETGLPRPLVVDSARSALELART
                     EIAGGGVPDVHRIASDLVAGLRRSALHKVINAAGVLLHTNLGRAPLSVRAAEAALEAA
                     VSYTNLELELTVGDRGGRGAYLTALLRTLTGAEDALVVNNNAAAVLLALAATASGRAV
                     PVARGELIEIGGSFRLPLVMEAGGARLVEVGTTNRTRLDDYITALQIHDCGALLKVHP
                     SNYRIEGFVTEVSVEEMASLARDRAIPLIHDLGSGLLDAETP"
     gene            823..1515
                     /locus_tag="VFU96_07475"
     CDS             823..1515
                     /locus_tag="VFU96_07475"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEU5113144.1"
                     /translation="MEAPLVEARPAATVMLIRDSEDGVEVLMVRRRPRGFFGGLTVFP
                     GGALDPVDDSDLASEVVPGDHPDQVFRAAALRELAEETGLALTNDGVVSAPEGRGESL
                     LAAMRGMDIHLDASALTLISRWVTPEGAPTRYDTRFYMADAGDTPDVRLDGDELVEHK
                     WVKPGDVLAIHADGGLEMFLPTIAHLRWLERWATVLDALSAAMGADGRSLVEPRRMED
                     GSFVPVHLPGDV"
     gene            1515..2288
                     /locus_tag="VFU96_07480"
     CDS             1515..2288
                     /locus_tag="VFU96_07480"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5113145.1"
                     /translation="MRIQRVLANNPGPFTGPGTNTWLVDDGGECIVIDPGPVDDRHRD
                     QILGQAAGLTPLGVLVTHTHADHAPLANPLARELGVPAYGYAPGPDFDPDLRLGEGSV
                     VDIGPSSLEVIHTPGHAEDHLCFRLVDVLFTGDHIMGGSSVMVEDMGSYLASLEKLRG
                     TGLSRLNPGHGEEMDHPDQVIDWYLAHRRQRHEEILEAIGGGAGSVDEIVEVVYHEVD
                     RALHPLAARSVEAHLGLLFEEGRIASSGHELVALPTNPT"
     gene            2324..3430
                     /locus_tag="VFU96_07485"
     CDS             2324..3430
                     /locus_tag="VFU96_07485"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEU5113146.1"
                     /translation="MALALPAIGQVTDEDIDRARDEVNEIMAEAQALGDEVQDAWAHQ
                     FALEREITDLTASIELARVKIVETEKKLEEVAVEMYMGSSTAASMQVLFSASDESYGA
                     GVEYLRNVTGNDADVINQLRSFRVELDRQTGRLEEASAEQEVLTAELEAKAGDLQGQL
                     VEAQAVYDGLVAQQAAEEAARRAAEEAARRAAEEAARNATTTTGPGSPDTTSGESNEP
                     TATTPPEETPDATPPPPSSSGGACPVAGAVSFSDSWGDARSGGRSHQGVDMIAARNTP
                     IVAIYSGRIQRLTDGNLSGLAIWLSSDGGDSFFYAHLESFAVSEGQSVSEGEVIGYNG
                     SSGNAPEFLPHLHFEYHPGGGGAVNPYPLVRDLC"
     gene            3672..4508
                     /locus_tag="VFU96_07490"
     CDS             3672..4508
                     /locus_tag="VFU96_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113147.1"
                     /translation="MTTEPPTPPESGGPIEPVPPPPSSEPGVPPAPPPPPPSSEPAVP
                     PPPPPPPPPQVPGGTPFQVGDAFNYGWKKFQEHLGPILIAMLIFLVVGGAVNFIATLL
                     GGGFGELTDPDDIGIGMFFGVGYLFFSLLSALVSLLIQAAVVRGALEITKGERIDLST
                     FLSTEHLGQVVLAAILLAIGTAIGLILCIIPGLLLIFFSMFTYQFILDKGLPAFDAIK
                     ASFALVNQNLGSVVGLFLASILAIIIGALLCGIGLIVAIPVTIIATAYAYRILSGQTV
                     VP"
     gene            4580..5179
                     /locus_tag="VFU96_07495"
     CDS             4580..5179
                     /locus_tag="VFU96_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003940105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HEU5113148.1"
                     /translation="MDPVDEILSQIPIDQLAQELGVDEGTAEQAVRQAVPALLGGMKA
                     NAEDPGGAASLERALGQHAATPTGGLDLGSVDTADGQAIVGNVFGDNSEQVINKLGSE
                     TGGSSVMAKLLPILAPIVLNMLAGKFLGQGQGQTAPGQATTSPGGLDDLIGSVTGSGG
                     AGGIGDLLGGLLGGGGGSGGSSDAIGDLLGGLLGGGTKS"
     gene            complement(5186..6325)
                     /locus_tag="VFU96_07500"
     CDS             complement(5186..6325)
                     /locus_tag="VFU96_07500"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEU5113149.1"
                     /translation="MVTLDDVIKTLEGVRDPELGGNIVELGMVTDVGIADSSVEVGIA
                     LTVAECPLRNQIENDTRRRVQAMPGVDHVQVRTTAMTKKQRAGLMSVARARARAGAEP
                     TQVSPTTRVIAIGSGKGGVGKSSISVNIAVAMAQAGFRVGLLDADIWGFSTPRMLGVE
                     DRLEADQDSKLIVPVEAQGVKLVSTGLIIETEETALMWRGLMLSKALEQFLKQVEWGD
                     LDYLVIDLPPGTGDIQMALTRMLPQAEMVVVTTPQRAAQKVAIRVADMARRSHMPVVG
                     VIENMAGLACACGRVHEVFGEGGGLELANDLGVPLIASIPLDPEVVPGGDQGMPVVAT
                     DTGSPAGVAMRDAAAKLVELVPPADLETCTGRIAKILIELEELPA"
BASE COUNT         1117 a         2097 c         2099 g         1138 t
ORIGIN      
        1 ggcgtctccg cgtccaggag gccggagccg aggtcatgga tcagcgggat ggcccggtca
       61 cgggccagcg aggccatctc ttcgaccgac acctcggtga cgaatccctc gatccggtag
      121 ttggagggat gcaccttcaa cagggctcca caatcatgga tctggagcgc cgtgatgtag
      181 tcgtcgagtc gagtgcggtt ggtggtcccg acttcgacga gacgcgcacc gccggcctcc
      241 atcaccaggg ggagccgaaa ggacccgccg atctcgatca actcccctct ggccacgggg
      301 acggcgcggc ccgaggcggt ggcagcgagg gcgagcaaga ccgcggccgc attgttgttg
      361 accacgagag cgtcttcggc tccggtcagc gttcgcaata gggcggtgag ataagccccg
      421 cgacccccgc gatcccccac agtcagctcc agctcgagat tggtgtagga gacagctgcc
      481 tcgagggcag cctcggcggc tcgcaccgac agcggggctc tgccgagatt ggtgtggaga
      541 aggactccgg cggcgttgat caccttgtgg agcgccgatc gccgcagccc ggcaaccaaa
      601 tcggaagcga tgcgatgcac atcgggcaca ccgccaccgg cgatctcggt gcgggccagc
      661 tcgagggccg atcgggcgga atccaccacc aacggtcttg gcaggcccgt ctccccgagc
      721 ccatcgacca gttggtcgac cggcgggagg ccccggtagg gatttttgct cgaagccacg
      781 gggcaagtct gccaggtcga tccttgtccg taggatgccg ggatggaggc gcctctggtc
      841 gaagcccgcc cagcggcgac ggtcatgctg atcagggatt ccgaagatgg ggtcgaggtg
      901 ctgatggtgc gccgtcggcc acgtggcttc tttggtgggc tcaccgtctt tcccggcggg
      961 gcgctcgacc cggtggacga ctccgacctc gcctccgagg tagtgcctgg cgatcacccg
     1021 gaccaggtgt tccgtgccgc ggctctccgc gagcttgccg aggagaccgg gctggccttg
     1081 accaacgacg gggtggtgtc tgccccggaa gggcgcgggg agagtctctt ggcggcgatg
     1141 cgggggatgg atatccatct ggacgcctct gccctcactc tcatctcgag atgggtcact
     1201 cccgagggtg ccccgacccg ctatgacacc cgcttttaca tggccgacgc cggcgacacg
     1261 ccggacgtcc gcctcgatgg cgatgagttg gtcgagcaca agtgggtcaa acccggggat
     1321 gtcctggcga ttcacgccga tggagggttg gagatgttcc ttcccactat cgcccatctc
     1381 cgctggctgg aacgatgggc aacggttctg gatgcccttt ccgccgcaat gggagcagac
     1441 ggtcgctccc tcgtagagcc ccgacgcatg gaggatggca gcttcgtgcc cgtccacctc
     1501 cctggcgacg tctgatgcga atccagcgcg tcctcgccaa caacccgggg ccgttcaccg
     1561 ggccgggaac caacacatgg ctggtggatg acgggggcga atgcatcgtg atcgaccccg
     1621 ggccggtgga cgaccgtcat cgggaccaga tcctggggca ggcggcgggg ctcactcccc
     1681 tcggagtcct ggtgacccac acccatgccg accacgcccc gctggcaaat ccgctggctc
     1741 gggagttggg tgttcctgca tacggctacg cccccggccc cgatttcgac cccgacctcc
     1801 gcctcgggga gggatccgtg gtcgacatcg gcccgtcatc gctcgaggtg atccacactc
     1861 cgggtcatgc cgaagaccat ctgtgttttc gattggtcga cgttctgttc accggggatc
     1921 acataatggg cgggtcctcg gtgatggtgg aagacatggg cagctatctg gcctctctgg
     1981 aaaaactccg cgggaccggc ctgagcagac tcaatcccgg tcatggcgag gaaatggacc
     2041 atcccgatca ggtgatcgat tggtatctgg cccatcgtcg acagcgacat gaggagatcc
     2101 tcgaggcgat cggtggaggc gcaggttccg tcgatgagat cgtcgaagtc gtgtatcacg
     2161 aggtcgatcg ggctctgcac cccctcgccg cccgctccgt ggaggcccac ctggggttgc
     2221 tgtttgagga agggcggata gcctcctccg ggcacgaact cgtcgcactg cccacgaacc
     2281 ccacgtgaca cgcttcgccc ggactctgct agctctgctc gtcatggctt tggcactccc
     2341 tgccatcggc caggtgaccg acgaggacat cgaccgcgcc cgtgacgagg tcaacgagat
     2401 catggccgaa gcgcaggctc tgggggacga ggtccaggac gcgtgggcac accagttcgc
     2461 gctcgagcgc gagatcaccg atctgaccgc ttcgatcgag ctggcgaggg tgaagatcgt
     2521 cgagaccgag aaaaagctcg aagaggtggc ggtcgagatg tacatgggat cgtccaccgc
     2581 cgcgtcgatg caggtcctgt tctcggcgag tgacgagagc tatggggccg gcgtcgagta
     2641 cctgaggaat gtgaccggca acgatgccga tgtgatcaac cagctgcgat cgttccgggt
     2701 ggagctcgat cgccagaccg ggcgtctcga agaggcttcc gccgagcagg aggtcctcac
     2761 cgccgagcta gaggcaaagg caggcgatct ccagggccaa ttggtcgagg cacaggcggt
     2821 gtacgacggt ctcgtcgcac aacaggcggc cgaagaggcg gcgcgacgtg ctgccgagga
     2881 ggcggcgcga cgtgctgccg aggaggcggc gcgcaatgcc accaccacga caggtccggg
     2941 ctcacccgac acaaccagcg gcgaatccaa cgagccaacg gcaaccacgc caccggaaga
     3001 gacacccgat gcgacacctc cgccaccgtc atcgagcggt ggggcatgtc cggtggctgg
     3061 ggccgtctcc ttctccgact cctggggcga cgcgcggtcg gggggccgtt cccatcaggg
     3121 cgtggacatg atcgcggcca ggaacacgcc gattgtcgcg atctactccg gcaggatcca
     3181 gagactcacc gacggcaatc tgagtggtct cgccatctgg ctcagctccg acggtggcga
     3241 cagcttcttc tacgcccatc tcgagagctt cgccgtcagc gaagggcagt cggtgtccga
     3301 gggcgaggtg atcggctaca acgggtcatc gggcaacgcg cccgagttcc tgccccatct
     3361 ccatttcgag tatcaccccg gtggcggggg agcggtgaac ccctatcccc tggttcggga
     3421 tctctgctga tctgcctctg aacgccgaaa gaggaccctt tcgggtcctc ttccgatctg
     3481 gcctccctgc ccctagcccc cacgcaccga ggcacgttct gtgggcatct tcggtcacgg
     3541 agagtggcgg tggaaggaca gttcggatta gcacacattg tgactagtca gttgaagaca
     3601 gttgcgaccc tggccgtcgc ggtggagcgt tagtcgttac cctctccgcc acctgacagc
     3661 ggagacgaca gatgaccact gagccgccca cgccgcccga gtccggaggg ccgatcgagc
     3721 cggtcccccc acccccatcg agcgagccgg gagttccgcc cgcaccgccg cctccacccc
     3781 catcgagtga gccggcggtt ccgcccccac cgccgccccc gcccccgcct caagtgccgg
     3841 gcggcactcc gttccaggtg ggagacgcct tcaactatgg atggaagaag ttccaggagc
     3901 acttgggccc catcctgatc gcaatgttga tcttcctcgt ggtgggcgga gcagtgaact
     3961 tcatcgccac cctcctcggc ggaggcttcg gagaactgac cgatccggat gacatcggca
     4021 tcggcatgtt cttcggggtc ggttacctgt tcttctccct cctcagcgcc ctggtgagcc
     4081 tgctcatcca ggccgcggtg gtacgtggtg cgctggagat caccaaggga gagaggatcg
     4141 atctcagcac tttcctgtcg acggagcacc tcggtcaggt ggttctcgcc gcgatcctgc
     4201 tcgcgatcgg gaccgcgatc ggcctgatcc tgtgcatcat ccccgggctt ctcttgatct
     4261 tcttctcgat gttcacctat cagttcatcc tcgacaaggg gctcccggcc ttcgatgcga
     4321 tcaaggccag cttcgccctg gtcaatcaga acctcggttc ggtggttggg ctgttcttgg
     4381 cctcgatctt ggccatcatc atcggcgccc tcctctgtgg gatcggcctc atcgtcgcca
     4441 tcccggtgac gatcatcgcc acggcctatg cctaccggat cttgagcggc cagacggtgg
     4501 ttccctgatc gcgctggttc caagctcggc gtaggctcgg ggcgcggttc gccacccggc
     4561 agccgcggaa ggaggtctca tggaccctgt agatgagatc ctgtcccaga tccccatcga
     4621 ccaactcgcg caagagcttg gcgtggacga ggggaccgcc gagcaggcag tgcgtcaagc
     4681 cgtgccggca ctgctcggag gcatgaaggc gaatgccgag gacccgggtg gcgctgcctc
     4741 actggagaga gccctcggcc aacacgccgc gaccccaacc gggggtctag atctcggatc
     4801 ggtcgacacg gcagatggcc aggccatcgt cggcaatgtc ttcggcgaca acagtgagca
     4861 agtgatcaac aagttgggca gtgaaaccgg cggctccagc gtcatggcca agctgctccc
     4921 catcctggcc ccgatcgtgc tcaacatgct ggccggcaag ttcctcgggc agggtcaggg
     4981 tcagacggca cccgggcagg caacgacatc ccccggtggg ctcgacgacc tgatcggtag
     5041 cgtcactgga tcgggtggag ccggaggcat tggcgacctg ctcggcggtc tgctcggcgg
     5101 tggtggaggg tctggaggct cctccgatgc gatcggcgac ctcctcggtg ggctgctcgg
     5161 aggcggcacc aagagctgac cttcgtcagg ccggcaactc ctccagctcg atgaggatct
     5221 tggcgatgcg accggtgcag gtctcgagat cggctggggg caccagctcg acgagcttgg
     5281 cggcggcatc ccgcatcgcg acaccggcgg gtgatccggt gtcggtcgca accacaggca
     5341 ttccctgatc gccaccaggg acgacctcgg gatcgagtgg tatggaggcg atcagaggca
     5401 cacccaggtc gttggccaac tcgaggccgc ccccctcacc gaacacttcg tggacgcggc
     5461 cacaagcgca ggcgagaccg gccatgttct caatgacacc gacgacaggc atgtgtgagc
     5521 gtcgtgccat gtcggctacc cgtatcgcca ctttctgggc ggcccgttgt ggcgtggtga
     5581 ccacaaccat ctcggcttgg ggcagcatcc gggtcagcgc catctgaatg tcgcctgtgc
     5641 cggggggaag gtcgatgacc aggtagtcga gatcgcccca ttccacctgc ttgagaaact
     5701 gctccagggc cttggacaac atgagcccgc gccacatgag tgccgtttcc tcggtctcga
     5761 tgatgagccc ggtcgacacg agcttcaccc cctgagcctc gacgggcacg atcaacttgc
     5821 tgtcctgatc cgcctcgagt cggtcttcga cacccagcat ccgcggtgtg gagaaccccc
     5881 aaatgtcggc gtcgagaaga cctacccgga aaccggcttg cgccatcgcg acggcgatgt
     5941 tgaccgagat cgatgacttg ccgacaccgc ccttcccaga tccgatggcg atgacccgcg
     6001 tcgtcgggga gacctgcgtc ggctcggcgc cagcccttgc cctggccctg gcgaccgaca
     6061 tgaggccggc gcgctgcttc ttggtcatgg cggtggttcg cacctggacg tgatctaccc
     6121 ccggcatggc ctgcacccgg cgcctggtgt cgttctcgat ttggtttcgc agcgggcatt
     6181 cggcgacggt gagggcgatg ccgacttcca ccgaggagtc ggcgatgccg acgtcggtga
     6241 ccatccccaa ctcgacgatg ttgccgccca actccgggtc gcggacacct tccagcgtct
     6301 tgatgacgtc atcaagggtg accattgccc tctcaatggt aatttccact accgatatag
     6361 ggggaatggt ccacgggccg gagacggtta atatggacga cacaggccac caagttcagg
     6421 accagagtta agagcgatga cacagattct c
//
LOCUS       DASTXO010000354         2191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_647775, whole genome shotgun sequence.
ACCESSION   DASTXO010000354 DASTXO010000000
VERSION     DASTXO010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647775"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VFU96_07505"
     CDS             complement(<1..383)
                     /locus_tag="VFU96_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5113150.1"
                     /translation="MPTFRPDVASLSPYVPGRSIEDVASESGLTPNEIVKLASNESPD
                     GPFPGVVEAAITALAESNRYPDDDLRELTAALSEYHAVPKDHLWFGSGSVGLLTHIAL
                     AAGGPGTSAVYAWPSFVMYRIASRWA"
     gene            497..>2191
                     /gene="pdhA"
                     /locus_tag="VFU96_07510"
     CDS             497..>2191
                     /gene="pdhA"
                     /locus_tag="VFU96_07510"
                     /inference="COORDINATES: protein motif:HMM:TIGR03181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEU5113151.1"
                     /translation="MIDRRGLLVGEDPELDPDLYQRMYRNMVLSRELDRRMLALQRQG
                     RIGTYAMLEGQEAVQIGSALALEADDFVFPSYREHGVQVTRGLPIEVLLAYWKGLPNA
                     RWDMEKYRTAIVSVPIATQLPHAVGYSYITKLRGESTVTAAYFGDGATSEVDFHSGMN
                     FAGVWKTPTVFICANNLYAISVPYEKQTASETIAQKAGAYGFDGVRVDGMDVVAVYLA
                     TRQAVTRARNGGGPTLIEAMTYRYGPHATADDPTLYRSREEEESWRQRDPIDRLRRFL
                     EERGEWDEQVEEKVAMETSDLVAAAITEIEAQPLPGRDDAVRHGFHRIPAHVVEQLHA
                     MQRSHDEAPTDFDPDEIWSIEHDAIPQMSTRSMTMAEAINAALHESMERDPGVIVLGE
                     DVGLTGGVFRVTEGLQERFGEARVIDSPLNESGIVGAAVGMALAGARPVAEIQFDGFV
                     YPAFDQIVSHLGRYRFRTRGTSEVPVVVRFPSGAGIGAHEHHCDSPEAYFVHAPGLVV
                     ICPSTPIDAKGLLASALTGHDPVIFLEPKVLYRAGREEVPVEHYTLPIGRARRRREG"
BASE COUNT          414 a          696 c          706 g          375 t
ORIGIN      
        1 gcccagcggg aggcgatgcg atacatcacg aacgacggcc aggcgtagac ggcgctcgtt
       61 ccgggaccgc ccgcggcgag ggcgatgtga gtgagcaggc ccacgctccc actgccgaac
      121 cagagatggt ctttcggaac cgcgtgatac tccgacagcg cggcggtgag ctcgcgcaga
      181 tcgtcgtccg gatagcgatt cgattcggcc agggccgtga tcgcggcctc gaccacgccg
      241 ggaaacgggc catccgggct ctcgttcgat gccagcttga cgatctcgtt aggcgtcaag
      301 ccgctctcag aggcaacgtc ctcgatcgat cggccgggga cgtacgggct gagagaggcg
      361 acgtccggcc gaaacgttgg catcgaaggg aaaggtaatg gtgtcgggac ggggttgttg
      421 ccgctccacc gaaagtaggg tttcgtcgga attggagaca ccgatcccag gcgagaacgc
      481 gataacgacc ctcgaggtga tcgatcggcg gggccttctc gtcggcgagg acccggagct
      541 ggaccccgac ctctatcagc gaatgtatcg caacatggtc ctctcccgcg agctcgaccg
      601 acgcatgctc gccctccaac gtcaggggcg gatcggcact tacgccatgc tcgagggaca
      661 ggaggcggta cagatcggat cggccctggc tctcgaagcc gatgacttcg tcttcccgtc
      721 ctatcgggag catggcgtcc aggtcacacg tgggctccct atcgaagtgc ttctcgccta
      781 ctggaagggt ctgcccaatg ctcgttggga catggagaag taccgcacgg cgattgtgtc
      841 cgtccccatc gcgacccaac tcccccacgc cgtcgggtac tcctacatca ccaagctgag
      901 gggcgaaagc accgtcaccg ccgcctactt cggcgatggg gccacatcgg aggtggattt
      961 ccactcgggc atgaactttg ccggggtgtg gaagacaccc accgtgttca tctgcgccaa
     1021 caatctgtat gcgatatcgg tgccctacga gaagcagact gcctctgaga cgatcgccca
     1081 gaaagccggt gcatacggat tcgacggcgt ccgggtggac gggatggacg tggtcgccgt
     1141 ctatctggcc actcgacagg ccgtcacccg ggcccggaat ggcggaggtc caaccctgat
     1201 cgaggcgatg acctaccgat atgggcccca cgccaccgcc gacgatccca cgctctaccg
     1261 atcgagagaa gaggaagagt cgtggcgcca gcgggatccg atcgaccgac tgcgccgatt
     1321 cctcgaggaa cgtggcgagt gggacgaaca ggtggaggag aaggtggcca tggagacctc
     1381 cgatctcgtc gccgccgcca tcaccgagat cgaagcacaa ccgcttccgg ggcgggatga
     1441 tgccgtccgg cacggctttc accggatccc cgcccatgtc gtggaacaac tccatgcaat
     1501 gcagcgctcc catgacgagg cgccaactga tttcgatccc gacgagatct ggtcgatcga
     1561 gcatgatgcg attccccaaa tgtcgactcg gtcgatgacc atggcggagg cgataaatgc
     1621 cgctctccac gagtcgatgg agcgcgaccc cggcgtcatc gtcctcggcg aggacgtcgg
     1681 cttgaccggt ggggtcttca gggtgaccga gggcctccag gagagatttg gcgaagccag
     1741 ggtgatcgac tcaccactga acgagtcggg catcgtgggg gccgcagtgg ggatggcgtt
     1801 ggccggggcc aggcccgtgg ccgagatcca attcgatggt ttcgtgtacc cggccttcga
     1861 ccagatcgtc agtcatctag gccgataccg gttccgcacc cggggcacgt ccgaagtccc
     1921 ggtggtggtg cgcttcccca gcggggccgg cattggggcc cacgagcatc attgcgactc
     1981 acccgaggcc tatttcgtcc atgcgcccgg actggtcgtg atctgcccat ccaccccgat
     2041 cgatgccaaa ggtctcctcg cttccgccct taccgggcac gatccggtga tcttcctcga
     2101 gcccaaggtc ctctaccgcg ccggtcggga agaagtccca gtcgagcatt acaccctccc
     2161 catcggtcgt gcccggagga ggagagaagg c
//
LOCUS       DASTXO010000355         2059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_353493, whole genome shotgun sequence.
ACCESSION   DASTXO010000355 DASTXO010000000
VERSION     DASTXO010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353493"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..327)
                     /gene="clpS"
                     /locus_tag="VFU96_07515"
     CDS             complement(46..327)
                     /gene="clpS"
                     /locus_tag="VFU96_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014375004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEU5113152.1"
                     /translation="MSHPAPVIHPDTGEDQDTDRPWQVIVWNDPVNTMSYVVYVLRKL
                     FGYDEPKATMLMLQVHHEGRAAVSDGPREKAEVDCYRLHGQGLWATMEQ"
     gene            complement(365..895)
                     /locus_tag="VFU96_07520"
     CDS             complement(365..895)
                     /locus_tag="VFU96_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113153.1"
                     /translation="MVALLVACTAGSDAPSATDAPATTTTSPPTTTTTVLSNVAVEAF
                     RTCLADRGIEIEEIPLDATGHPRLDLVMATLDFGDPETAEAVSVCSEHLETGALDLGD
                     DDVLRQAILQQLQEFSQCMVDLGVEDFPDPVPGFTGVGSPFPVAEIPYSDPGFATAVV
                     ACRDALLEALAGADEG"
     gene            complement(922..>2059)
                     /locus_tag="VFU96_07525"
     CDS             complement(922..>2059)
                     /locus_tag="VFU96_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEU5113154.1"
                     /translation="EMVIKTGEVAASVAGVSPPVSSLAVLDGEGHLVDRVQPEEVFAG
                     VACRACDEICPVNIEILDRILDMRRYLTLMESEFPSELGRAFVSMENQANPWGLSRQG
                     RADWTKELDFEVPIFGENGKVSADYLWFIGCAGSYDDRNTAVSVSLARLLHRAGIDFA
                     ILGSREACTGDPARRAGNELVYQQLALENIATFDEIGVTRVITQCAHCFNAIANEYPQ
                     LGGSYEVVHHSQLLAELLTQGRLEAPVRADGAARRIAYHDPCYLGRHNDIYRAPREVA
                     GSGSMELVEMPRHGTRALCCGAGGARFWMEEQTGKKINIERAEEALATGADEIAVACP
                     FCFVMLDDGVKELGREDVRVRDLAMILADGLTEGAAEGHSAGSD"
BASE COUNT          349 a          682 c          680 g          348 t
ORIGIN      
        1 tgagatcgac gtcgatccca ccccgggccg acttgaaccg gcgcatcact gctccatcgt
       61 ggcccacagc ccctggccgt gaaggcggta gcagtcgact tcggctttct ccctgggtcc
      121 atcggaaacc gctgctcggc cctcgtgatg cacctggagc atgagcatcg tggcctttgg
      181 ctcgtcgtaa ccgaagagct ttcgcaggac atagacgacg taggacatgg tgttgaccgg
      241 gtcgttccag acgatgacct gccagggacg gtctgtgtcc tggtcctcgc cggtgtctgg
      301 atgaatgacg ggagcggggt gtgagatcac caggggcgcg atcgtaggcc gaatcccggg
      361 ccactcaccc ctcgtcggcg cccgccaatg cctcgaggag cgcatcccgg cacgcgacca
      421 ccgctgtggc gaagccgggg tcggaatatg ggatctcggc aaccgggaac ggggagccga
      481 caccggtgaa cccaggaacc gggtccggga aatcctcgac tccgagatca accatgcatt
      541 ggctgaactc ctgaagctgc tggaggatgg cctggcggag cacgtcgtcg tcacccaggt
      601 ccagggcccc cgtctcgagg tgctctgagc agacggagac cgcctccgcc gtctccgggt
      661 caccgaagtc gagggttgcc atgaccaggt cgagacgcgg gtggccggtg gcgtcgagcg
      721 ggatctcctc gatctcgatc cccctatcgg cgagacaggt gcgaaaggct tccacggcca
      781 cattcgacag cacggtggtg gtggtggtcg ggggactcgt cgtggtcgtc gccggtgcgt
      841 cggtggcgga tggcgcgtct gagcctgccg tgcacgcgac caggagcgcg accatcagca
      901 aggtgaggac ggaccgtcgg atcagtcgct cccggcggag tgaccttcgg cagctccctc
      961 ggtgaggcca tcggcgagga tcatcgccag atcccggacc cgcacgtcct cccggcccag
     1021 ttccttgacc ccgtcgtcga gcatgacgaa gcaaaacggg cacgcgaccg cgatctcgtc
     1081 cgccccggtg gcgagagcct cctcggccct ttcgatgttg atcttcttgc cggtctgctc
     1141 ctccatccag aacctggcgc ctcccgcgcc acagcacagg gccctggtgc catgacgggg
     1201 catctctacc aactccatcg accccgatcc ggcaacctcc cgaggggcgc gatagatgtc
     1261 gttgtgcctc ccgagatagc aggggtcgtg gtaggcgatc cgcctggcag ccccgtcggc
     1321 ccggaccggg gcctcgagtc ggccctgcgt caggagctcg gccagcaact ggctgtgatg
     1381 gaccacctcg tagctgcccc cgagctgggg gtactcgttg gcgatggcat tgaagcaatg
     1441 ggcgcactgg gtgatcaccc gggtgacccc gatctcgtcg aaggtggcga tgttctccag
     1501 agccagctgc tggtagacga gctcgttccc ggcgcgtcgc gccgggtcgc cagtgcaggc
     1561 ctcccttgac ccgaggatgg cgaaatcgat gccggctcga tggagaagac gggccagtga
     1621 cacggagacg gcggtgtttc ggtcgtcgta cgagccggca cacccgatga accacaggta
     1681 gtcggcgctg accttgccgt tctcgccaaa gatcgggacc tcgaagtcga gctccttcgt
     1741 ccagtccgcc cgcccttggc ggctcaggcc ccacgggttg gcctggttct ccatggagac
     1801 gaaagcgcgc ccgagctcgc tggggaactc cgactccatc agcgtcagat agcggcgcat
     1861 gtcgaggatt cgatccagga tctcgatgtt gaccgggcag atctcgtcgc aggcccggca
     1921 cgcgacaccg gcgaacacct cctctggctg gacccggtcg actagatgtc cttcgccgtc
     1981 gagcaccgcc agcgaagaaa ccggggggga gaccccggca accgaggcgg caacctcacc
     2041 ggtcttgatg accatctcc
//
LOCUS       DASTXO010000356         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1495174, whole genome shotgun sequence.
ACCESSION   DASTXO010000356 DASTXO010000000
VERSION     DASTXO010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495174"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /gene="hrcA"
                     /locus_tag="VFU96_07530"
     CDS             complement(<1..856)
                     /gene="hrcA"
                     /locus_tag="VFU96_07530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU5113155.1"
                     /translation="MLEDRRSEILRALVEEHIRSGEPVSSRAIVDRAHLSVSSATVRN
                     DLAALESEGFVIQPHTSAGRIPTAAAYRYYVDHLGPGRLRSTAQAKINSFFGEVHLEL
                     SKLLKETSDLLAEVTDLPAVVVAPALRGDRVRDLHSVQVTADQMLLIVVTDGGRVLQQ
                     RARVRVPITATELEEAHRVVSAAVVGSDLAATPMISDEVLGRLDERTREAVLTMNDGL
                     HLAAAAGTEIFIGGTQRLTTVWEDLSAVQRILEVLAREAEVMKILAVASGTSIQIGAE
                     LPLQDVDLA"
     gene            complement(862..2037)
                     /gene="hemW"
                     /locus_tag="VFU96_07535"
     CDS             complement(862..2037)
                     /gene="hemW"
                     /locus_tag="VFU96_07535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEU5113156.1"
                     /translation="MKPLEVDTHAAMSAAYVHIPFCSAICPYCDFAVVAGADDRIDRY
                     VDCVITEIGISDPWRELEAVYFGGGTPSHVGSHLLGRVLDALRKRHGIAPDAEISLEA
                     NPEDFDQERAEELLSAGFNRVSFGAQSFDDEVLARLGRRHRAEDIDGSVDHARRAGFD
                     NVSLDLIFGTPLETGDSWATSLQHAVELGPDHVSCYALTVEPGTPLHREVKAGAEAPD
                     PDTQADRYELAEQLLPLAGLDRYEVSNWSRPGRQCRYNLVVWAQGEYEAYGNGAHRFQ
                     GGVRSHNVRRLEAYMERVEAGTRPIMGVDPIEGWDVEIDRLFVGLRRTVGVGPGPGVE
                     SLLQTGGGQMLLREGVIEVADGRLVVRRPLLTDEVHRQVLDLAPPQGWVESLNADNL"
BASE COUNT          384 a          722 c          658 g          364 t
ORIGIN      
        1 cggcgaggtc gacgtcctgc aacggaagct cggcgccgat ctggatcgac gtccccgaag
       61 cgaccgccag gatcttcatg acctcggcct cacgagccaa aacctcgagg attctctgaa
      121 ccgcgctcag gtcctcccac acggttgtca ggcgctgggt tcccccgatg aagatctcgg
      181 tgcctgccgc tgcagccaga tgaagcccgt cgttcatggt gagcacggct tcgcgggtgc
      241 gttcgtcgag tcggcccagc acctcatcgc tgatcatcgg agtcgcagca aggtcggacc
      301 cgacgaccgc ggcgctgacc acccgatggg cctcctccaa ttcggtggcg gtgatgggca
      361 cacgaaccct ggcccgctgc tgaagtaccc ggccaccgtc ggtcacgacg atcagaagca
      421 tctggtcggc cgtcacctgg acgctgtgca gatcacgaac ccgatctccg cgcagagccg
      481 gcgccacgac cacggcagga agatcggtca cctctgccag gagatccgaa gtctctttga
      541 gaagcttgga cagctcgagg tggacttcgc cgaagaagga attgatcttc gcttgcgccg
      601 tcgatcgaag ccgccccggg ccgagatgat cgacgtagta gcggtacgcc gccgcggtag
      661 ggatcctgcc ggcggaggtg tggggctgga tgacgaatcc ctccgactcg agcgcggcga
      721 ggtcgttcct cacggtggca ctcgacaccg agaggtgggc ccggtcgacg atggcgcgag
      781 agctgaccgg ctcccccgag cgaatgtgct cctcgaccag ggcgcgaagg atctcggacc
      841 ggcgatcttc aagcatttcg actagaggtt atccgcgttg agcgattcca cccacccctg
      901 gggtggtgcg agatcgagca cctgacggtg tacttcgtcg gtcagcaacg gtcgtcggac
      961 aacgagtctc ccatcggcga cctcgatgac cccctcccgg agcagcatct gccctccgcc
     1021 ggtctggaga agggattcga cccctggccc gggaccgaca cccacagttc gccgcaggcc
     1081 gacgaacaga cggtcgatct cgacatccca tccctcgatc ggatcgacac ccatgatcgg
     1141 cctcgtaccg gcctcgaccc gctccatgta ggcctcgagc cggcgcacat tgtgacttcg
     1201 caccccgccc tggaaccggt gggccccatt cccgtaggcc tcgtactccc cctgtgccca
     1261 gaccaccagg ttgtacctgc attgccgacc aggccgggac cagttggaca cctcatatct
     1321 atccaggccg gccaggggga ggagctgctc tgcgagctcg tagcgatcgg cctgggtgtc
     1381 gggatccggg gcttcggccc cggccttcac ctctcgatgt agcggggtgc ccggctcgac
     1441 cgtcagggca taacaagaca cgtggtcggg gccgagttcc accgcgtgtt gcagcgaagt
     1501 cgcccacgaa tctcctgtct ccaagggagt cccgaagatc aggtcgagcg agacattgtc
     1561 gaaacctgcc cggcgtgcgt ggtcgacaga tccgtcgatg tcctccgctc ggtgacgccg
     1621 gcccaagcgc gccaggacct cgtcatcgaa gctctgggcg ccaaacgaca cccggttgaa
     1681 cccggccgaa agcaactcct ccgcccgctc ctgatcaaag tcctcggggt tggcctccag
     1741 cgagatctcg gcatcaggtg cgatgccgtg acgcttccga agtgcgtcga ggactcgacc
     1801 gagaagatgc gagccgacgt ggcttggtgt gcccccgccg aagtacaccg cctcaagctc
     1861 ccgccaaggg tccgagatcc cgatctcggt gatgacgcag tccacgtagc gatcgatccg
     1921 atcatcggcg cccgcaacga cagcgaagtc gcagtagggg cagatagccg agcagaacgg
     1981 tatgtgaacg tacgcagcgc tcatcgcggc gtgggtgtcg acctcgagag gtttcatgcg
     2041 tcgtcgtcga cgcggagggc ggccacgaat gcctcttggg gcacctccac cgagcccacc
     2101 atcttcatcc gcttcttgcc ttccttct
//
LOCUS       DASTXO010000357         2596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1384579, whole genome shotgun sequence.
ACCESSION   DASTXO010000357 DASTXO010000000
VERSION     DASTXO010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384579"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..1958
                     /gene="dxs"
                     /locus_tag="VFU96_07540"
     CDS             105..1958
                     /gene="dxs"
                     /locus_tag="VFU96_07540"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEU5113157.1"
                     /translation="MSILESISGPADLRGLSKEELDVLADEIRTFILDKVSKTGGHLS
                     PNLGVVELTLALHRVFDSPNDRFIWDVGHQAYVHKLVTGRMEGFDHLRHTGGLSGYPS
                     REESDHDFVENSHASTSLSYALGHALAGYPGYTVAIIGDGALTGGMAYEALNHIAVTQ
                     PERLIIVVNDNGRSYAPTVGGLAALATLAHYRFDPRYERTKRTLGRMLRGLPLVGETA
                     DELAGRLKDAVKQILEPFTVFDALDLKYTGQIDGHDIELLEETFQAAKHFDRPVVIHV
                     VTQKGNGYQPAIEDELEKLHGVGSFDLATGKAIKEELRLTDVAGNAVAEAAASRPDLV
                     GISAAMVTTAGLMQMWQRFPDRVFDTGIAEQHAVTLAAGMALAGKRPVVAVYSAFLQR
                     AFDQIIADVALHDLPVTFLVDRSGITGPDGPSHHGVFDLSYLRLIPNMVVGVPADATE
                     LCAMIETAVDHDGPIAIRYPKGAAVSVPAVPVAALAVGEAQLLSEGNDVLLVAAGRMV
                     EVAEKAAVDIRTKGFSVGVVNARWVKPLDPRILEWAAPARHVVTLEDNVVAGGFGAAV
                     MEAFAAAGLVKEITCIGVPDHFLPFGSPSDVAQLVGLDPDSVASTVVALLG"
     gene            complement(1955..>2596)
                     /locus_tag="VFU96_07545"
     CDS             complement(1955..>2596)
                     /locus_tag="VFU96_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018313773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU5113158.1"
                     /translation="RSGSGKSTLLRCINFLEEPTSGTVEVGGIRLEPGPRTHRQREAI
                     RSVRIKAGMVFQDFNLFPHLTVLQNVIEAPVTVKGQERAHAVAVAEELLAQVGLADKK
                     DEHPIRLSGGQKQRVAIARALAMEPEVMMFDEPTSALDPELISEVLSVMKSLATETHM
                     TMIVVTHEMGFAREVATTMAFFHEGNIVEKGPPNSVIADPQHAQTKRFLEAVL"
BASE COUNT          440 a          857 c          816 g          483 t
ORIGIN      
        1 tctcggtcga gagcgagttg ggtgccggct cgacgttcac cgtccatttg ccggtcctcg
       61 aacggcaaat tcctgcatcc cactagcctg aaccggttcc aaccatgtcg attctcgaat
      121 ccatctctgg tcccgcggac ctgcgtgggc tgagcaaaga agaactggac gttctcgccg
      181 atgagatccg caccttcatc ctggacaagg tgtccaagac cggcgggcat ctctccccga
      241 acctcggtgt tgtcgagctg acccttgcct tgcaccgcgt gttcgactcg cccaatgatc
      301 ggttcatctg ggacgtcggc caccaggcct atgtccacaa attggtcacg ggacgcatgg
      361 agggcttcga ccatctccgc cacaccggtg ggctgtcggg ctacccgagc cgtgaggagt
      421 ccgatcacga cttcgtcgag aacagccacg cgtcgacctc cctttcctat gccctgggcc
      481 acgctctcgc cggctatccc gggtacaccg tcgccatcat cggggacggc gccctcaccg
      541 ggggcatggc ctacgaggcg ctcaaccaca tcgcggtgac tcagccggag cgactgatca
      601 tcgtggtcaa tgacaacggc cggtcatacg ccccgacggt gggtggcctc gccgccctcg
      661 ccaccctggc ccattaccgc ttcgaccccc gttatgagag gaccaagcgc accctgggcc
      721 ggatgctgag agggctccca ttggtcggcg agaccgccga cgagctggct ggacggctga
      781 aggacgcggt caagcagata ctggagccct tcaccgtctt cgatgcactc gacctcaagt
      841 acaccgggca gatcgacggg cacgacatcg agctgctcga ggagactttc caagccgcca
      901 agcactttga ccgcccggtc gtgatccacg tcgtcactca gaaaggcaac ggctaccaac
      961 ccgccatcga agacgagctg gagaagctgc atggtgtggg ctcgttcgac ctggccaccg
     1021 gcaaggccat aaaagaggag ctcagactca ccgacgtcgc cggtaacgca gtcgccgagg
     1081 ccgccgccag caggccggac ctcgtgggta tatcggcggc gatggtgact actgccgggc
     1141 tgatgcagat gtggcagcgg tttccggatc gagtgttcga caccgggatc gccgaacagc
     1201 atgcggtgac gctggccgcc gggatggccc tggccggcaa acgcccggtg gtcgccgtct
     1261 attcagcctt ccttcagcga gccttcgacc agatcatcgc cgacgtcgcc ctccacgacc
     1321 ttccggtgac attcctcgtg gatcgatcgg ggatcaccgg gcccgatggg ccctcgcatc
     1381 acggggtgtt cgatctctcc taccttcgcc tgatccccaa catggtcgtc ggggtgcctg
     1441 ctgacgccac cgagctctgc gcgatgatcg agacggcggt ggaccacgat ggtccgatcg
     1501 ccatccgcta cccgaagggg gcggcggtgt ccgtcccggc tgtccccgtt gccgcgctgg
     1561 cggtgggtga ggcgcagctg ctctccgaag ggaatgatgt cctcctggtg gcggcgggcc
     1621 ggatggtgga ggtcgcggag aaagccgccg tggacatcag gaccaagggg ttctcggtgg
     1681 gcgtggtgaa cgcgcgatgg gtgaagccgc tcgatccccg gatcctcgaa tgggcagccc
     1741 cggctcgcca cgtagtgact ctcgaggaca atgtggtcgc cgggggattt ggcgccgcgg
     1801 tgatggaggc attcgccgcg gcaggtctgg tcaaggagat cacctgtatc ggggttcccg
     1861 accacttcct ccccttcggc tccccgtctg acgtagccca gttggtgggt ctcgatcccg
     1921 actcggttgc gtcgaccgtc gtcgccttgc tcggctagag aaccgcttcc aggaacctct
     1981 tggtctgggc atgctggggg tcggcgatca ccgagttcgg cgggcccttt tcgacgatgt
     2041 tcccttcgtg gaaaaaggcc atcgtggtgg ccacctcgcg ggcgaaaccc atctcatgag
     2101 tgacgacgat catcgtcatg tgcgtctcgg tcgccaggct cttcatcacc gaaagcacct
     2161 ctgatatcaa ctcggggtcg agcgccgagg ttggctcgtc gaacatcatc acctcaggct
     2221 ccatggccag ggctcgggcg atggcgaccc gctgcttctg ccccccggac agccggatcg
     2281 ggtgctcgtc cttcttgtcg gccaaaccca cctgggcaag gagctcctcg gccaccgcga
     2341 ccgcatgggc gcgctcctgg cctttgaccg tgaccggggc ctcgatgaca ttctgaagga
     2401 cggtcaagtg ggggaagagg ttgaagtcct ggaacaccat ccccgctttg atccggacac
     2461 ttcggatcgc ctctcgctgg cgatgggtcc ggggcccggg ctcgagccga attcctccga
     2521 cctccaccgt ccccgaagtt ggctcctcca agaagttgat gcagcgaaga agagtgctct
     2581 tgcccgagcc ggagcg
//
LOCUS       DASTXO010000358         3628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_353699, whole genome shotgun sequence.
ACCESSION   DASTXO010000358 DASTXO010000000
VERSION     DASTXO010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353699"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VFU96_07550"
     CDS             <1..666
                     /locus_tag="VFU96_07550"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5113159.1"
                     /translation="TEPAGLDVLGSMLGEDVTLVHCCDFDDPDLDTVASSGAHIVLTP
                     SSEMAAGLQPLQIQKLVDRGIRPGLGVDTERMAPGDIFAQMRAAISLQHATLFDMKLA
                     GKAGLPKLLSTRDVIRYATIDGAAAVGLGSATGSLAPGKQADIVILRADTPNIYPVND
                     AIGAVVWGMDTSNVDWVFVAGRPVMRNGVLDADLDRARGLAAAAQQRVAPPLVEIGVG
                     GRG"
     gene            663..1709
                     /locus_tag="VFU96_07555"
     CDS             663..1709
                     /locus_tag="VFU96_07555"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5113160.1"
                     /translation="MTQTRVPSSAVTSFVVGSRYMPVYLALILLFVVASIWAPATLSS
                     AGLSEIAPLGTFLAITALGQMLVIMTGGIDLSVPGTFLLGGLLMVGVGQQSDERMWIA
                     YLTAVGAAVVIGLLNGILIGGLKLNALIVTLAVGQIVVGVANRYYTTVAIQKPVPPAL
                     SEWANGTYLGVTPIFWIGVGLAIVLMVLFRFTGLGRRFQVVGANPVASNVLGLRVNLN
                     QMLAYVVAAVLYATAGVLLAADLRAPGVTVGAPYLLGPIAAVVIGGASLTGGLASPIS
                     TWAAAFFRAGLNQMMRVLGLPTALQFVVFGLVIIGGMLVSGDRIIKAVERVLRDRSRP
                     RRREGRLAVEGGEP"
     gene            1778..3127
                     /locus_tag="VFU96_07560"
     CDS             1778..3127
                     /locus_tag="VFU96_07560"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU5113161.1"
                     /translation="MKPRVIALIAVLALVLAACTGDVAETSTTAAPEEEPATTVAAEP
                     TTTAAGEVTTTAATEEDPLAAYGETTAADPALVETALGPVDEVPDIVLASIARASQDL
                     DQATIDKAMECFTNAVECDTGTGGEVIMGYADGGGLNVWRQVTRMEAILQALTYPEIG
                     TMVFRDAQWSTDPAVPIGDINYMVERGVTFIIGYPDWGVALGDAIKAAEAAGIPYVPY
                     SAGWVGLPGQDGALTPGTDYLSIVGEELCALGESFADIINTGVGEGQIGVLGGTPGNA
                     LSLGWQQCHIPALGPGIEVVNPPADPNSTTGDTYWANDIAAEVVSGMLSANPDIGGFS
                     YEYADGLYTALEGVEIDELTLAIRTDEQTLFCDWAERNNPNYHIWYSAGGNFQSRTAV
                     TAQMMNLQGAEIPAEIVVPHVMREVTAADCNPDRVTPTVSNTSLVPDSVLALMYPEG"
     gene            3124..>3628
                     /locus_tag="VFU96_07565"
     CDS             3124..>3628
                     /locus_tag="VFU96_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5113162.1"
                     /translation="MSIGGPGGGPATAETVLELAGVSRQFGAVRALTDVSFDCRAGEV
                     HALVGENGSGKSTLLGIASGFVEPDRGTVRIAGKTLRTDSPALARKLGLAMAYQDTSL
                     VLAQPVKNNLYMAAPPDERPPFWRRKKWARRLLGEFDLDMELFPDSPAGFLTLAERQM
                     FEVAKALV"
BASE COUNT          623 a         1180 c         1200 g          625 t
ORIGIN      
        1 accgagccag ccggcctcga tgtgctgggg tcgatgctcg gtgaggacgt cactctggtg
       61 cactgctgtg acttcgacga tcccgacctc gataccgttg ccagctctgg tgcgcacatc
      121 gtcctgactc cgtcgagcga gatggcggcc gggctgcagc ccctgcagat ccagaagctc
      181 gtcgatcgcg gcatccggcc cgggctgggc gtagacaccg agagaatggc cccaggcgac
      241 atcttcgccc agatgcgtgc cgccatctct ctgcagcatg caacgctctt cgacatgaag
      301 ctcgcgggca aggccggcct ccccaagctc ctcagcaccc gtgacgtgat ccgctacgcc
      361 accatcgacg gtgccgccgc ggtcgggctg ggaagcgcca cggggtcgct ggcccccggc
      421 aagcaggctg acatcgtcat cctgcgcgcc gatacgccca acatctatcc ggtcaacgac
      481 gcaatcgggg ccgtggtatg ggggatggac acatcgaacg tcgactgggt gttcgtcgcc
      541 gggcggccgg tgatgcgcaa cggggtcctc gatgcggacc tggatcgagc acggggcctg
      601 gcggccgccg cgcagcagcg tgtggccccc cctctcgtcg agatcggcgt cggggggagg
      661 ggatgaccca gacccgagtc ccgtcatcgg ccgtgacatc cttcgtggtg ggctccaggt
      721 acatgccggt gtacctggca ctgatcttgt tgttcgtagt ggcctccatt tgggcgccgg
      781 ccaccctcag cagcgccggc ctcagcgaaa ttgctccctt gggcacgttc ctcgctatca
      841 ccgcactcgg ccagatgctg gtgatcatga ccggggggat cgacctgagc gtgcccggga
      901 ccttcctgct cggcggcctg ctcatggtcg gtgtgggcca gcaatcggac gagcggatgt
      961 ggattgcata cctcaccgcc gtcggggccg cggtcgtgat cggcctgctc aacgggatcc
     1021 tcatcggtgg gctcaagttg aacgccctga tcgtgaccct cgcggtagga caaatcgtgg
     1081 tgggtgtcgc caaccgctac tacaccaccg tggccatcca gaaaccggtt ccgccggccc
     1141 tctcggagtg ggccaacggg acctacctcg gtgtgactcc tatcttctgg atcggagtgg
     1201 ggctggccat cgtcctgatg gtgttgttca gattcaccgg actggggcgg agattccagg
     1261 tggtcggggc caacccggtg gcctccaacg tgctcgggct gagggtgaac ctgaaccaga
     1321 tgttggccta tgtggtcgcc gcagtcctct acgccaccgc cggcgttctc ctcgccgccg
     1381 acctccgcgc cccgggggta accgtcgggg ccccctacct cctcggcccg atagccgccg
     1441 tcgtgatcgg cggcgcctct ctcaccggag ggctggcgag ccccatctcc acctgggcgg
     1501 cagctttctt ccgagctggg ctcaaccaga tgatgagggt gctgggcctt cccaccgcac
     1561 tccagttcgt ggtgttcggc ctggtcatca ttggcgggat gttggtctcg ggagaccgga
     1621 tcatcaaagc cgtcgaaagg gtcctgcgtg accggtctcg accgcggcgc agggaaggaa
     1681 ggctcgccgt cgaaggcggc gaaccgtgag aaaaggagaa tcgacgatcg aacccgaaag
     1741 cgggagagca acaaaacgaa gagaacggag gaggatgatg aaacccaggg ttatcgccct
     1801 gatcgccgtg ttggcactcg tcctggccgc ctgtacgggc gatgtagccg agaccagcac
     1861 caccgcggca ccagaagagg agccggcgac caccgtcgca gccgagccaa ccaccaccgc
     1921 ggctggtgag gtgaccacga cagccgccac ggaagaggac cccctcgccg cctacggcga
     1981 gaccacggca gcagacccgg cgctggtgga gacggcactc ggaccggtgg acgaggttcc
     2041 ggacatcgtc ctggcgtcca ttgcccgggc gagtcaggat ctcgatcagg ccaccatcga
     2101 caaggcgatg gagtgcttca ccaacgccgt cgaatgcgac accggcaccg gtggcgaggt
     2161 catcatgggt tacgccgacg gcggcgggct caacgtgtgg cgtcaggtga cacgcatgga
     2221 ggccatcctc caagctctca cctatccgga gatcgggacc atggtgttcc gcgacgccca
     2281 gtggtctaca gacccggcgg ttcccatcgg cgacatcaac tacatggtgg agcggggagt
     2341 cacgttcatc atcgggtatc ccgactgggg cgtcgccctc ggtgacgcaa tcaaggccgc
     2401 cgaggcggcg ggcatcccgt atgtccccta ttcggcggga tgggtcggcc ttcccggtca
     2461 ggatggggca ctgacccccg gcaccgacta cctctccatc gttggcgagg agctgtgcgc
     2521 cctgggtgag agcttcgcag acatcatcaa caccggggtc ggggaaggac agatcggtgt
     2581 cctcggtggc acacccggca acgccctttc gctcgggtgg cagcagtgcc atatcccggc
     2641 gctgggcccg gggatcgaag tggtcaaccc gcccgcagat ccgaactcga ctaccggtga
     2701 cacgtattgg gccaatgaca tcgccgccga ggtcgtaagc ggcatgttgt cggcgaatcc
     2761 cgacatcggc gggttcagct atgagtacgc cgacgggctc tacaccgcat tggaaggggt
     2821 ggagatcgac gagttgacgc tcgcgatccg gaccgatgag cagacactgt tctgtgactg
     2881 ggcggagcgc aacaacccga attaccacat ctggtactcg gctggcggca acttccagtc
     2941 acggactgcc gtcactgcgc agatgatgaa tctccaaggg gccgaaatcc cggccgagat
     3001 cgtcgtcccc cacgtaatgc gtgaggtcac ggcggcggac tgcaaccctg accgtgtcac
     3061 cccgaccgtg tccaacacgt cgttggtccc ggactcggtt ctcgcgttga tgtatccgga
     3121 aggatgagca tcggcggtcc cggaggaggg ccggccaccg cggaaaccgt cctcgagctg
     3181 gcgggtgtat cccgccagtt cggggcggtc cgtgcgctga ccgacgtcag cttcgattgc
     3241 cgcgccggcg aggtacatgc tctcgtcggc gagaacggat cggggaagtc gaccctcctc
     3301 gggatcgcta gcggattcgt cgagcccgat cggggcacgg tgcgcatcgc cgggaagacc
     3361 ctgcgcaccg attccccggc tttggcccgc aagctcggtc tggccatggc ctatcaggac
     3421 acctcgctgg tcctggctca accggtgaag aacaatctct atatggcggc gcccccggac
     3481 gagaggccac cattctggag gcgaaagaag tgggcgcggc gcctcctcgg cgagttcgac
     3541 ctcgacatgg agctgtttcc cgattctccg gcgggttttc tcaccctggc cgagcgccag
     3601 atgttcgagg tggccaaggc gctggtct
//
LOCUS       DASTXO010000359         3300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1274300, whole genome shotgun sequence.
ACCESSION   DASTXO010000359 DASTXO010000000
VERSION     DASTXO010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274300"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..392
                     /locus_tag="VFU96_07570"
     CDS             165..392
                     /locus_tag="VFU96_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113163.1"
                     /translation="MSDAEFAWEPVDGSWRVFRDDEGRCATTTRIPTSQPAPFTTIGW
                     RLVHIALCKVMHHECAFGPRELNVDHDRDPR"
     gene            760..1206
                     /locus_tag="VFU96_07575"
     CDS             760..1206
                     /locus_tag="VFU96_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113164.1"
                     /translation="MRARWSDAWILAAVDWAADADLAKIIQAADAINHAIPTIEEVEH
                     AIQFLGAAGLVRYSDHSLQLTDEGRRVCSRTEAQDIFTTLDRLHAFLKKFPEVNDASN
                     AESVLDRSTYISALERYETLMVEELPELYGRGTSSANRDGDDGSTA"
     gene            complement(1447..1791)
                     /locus_tag="VFU96_07580"
     CDS             complement(1447..1791)
                     /locus_tag="VFU96_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEU5113165.1"
                     /translation="MPHTDLWTFQASLPGTREALVGSDVEARDGSIGKVDEATPDTGR
                     AYIVVDTGPWIFGRKVVIPAGTVDRIDLDDKKIFVALTKDQIKDSPEYDPAQFDEDYR
                     TRLGTYYEPYLH"
     gene            complement(2065..2565)
                     /locus_tag="VFU96_07585"
     CDS             complement(2065..2565)
                     /locus_tag="VFU96_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113166.1"
                     /translation="MPTRTAHSVRSSSQCPRLDVHPELLARRSPFEQVAGFEGLTPGI
                     EGLTSLKENRSSVMGFREERIAKNEAVVREINESIQQAHGSAPRSSFMHIVCECGYEE
                     CDSIIALTKDEYERIRNDPRQFCVIEEHVIQDVEIVVEDNDRFAIVAKRQGTPAEVAI
                     RTDPRG"
     gene            2867..>3300
                     /locus_tag="VFU96_07590"
     CDS             2867..>3300
                     /locus_tag="VFU96_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5113167.1"
                     /translation="MNAPASITITPVLVADLHVGGERMPVYVHVIDHPDARVLVDTGM
                     TELHPAVADLDPRLRPLSEQDFDLAGIDIVVNTHLHYDHCGGNHLFAGRPIYVQRREL
                     EDALGEDDYTIREWVEAPGVRYVPVDGELELLPGLRLVPAPG"
BASE COUNT          592 a         1065 c         1037 g          606 t
ORIGIN      
        1 gaatcgtgac gcgcagccgc cgcctgcggg gagcgcctgg acgcgctggg accggctcca
       61 cgcgccaagt cgcgtaccgt cgtgggcgag gtggtcacga ccccggacgt ccttgctcgc
      121 cagatggccg aggcctacga cgagctagga cagaggctct gggcgtgtca gatgccgagt
      181 tcgcctggga acccgtggac ggaagctggc gcgtgttccg ggacgacgag ggccggtgcg
      241 cgacgactac gcgcattccg acctcccagc cggccccgtt caccacgatc ggctggcggc
      301 tcgtccacat cgccttgtgc aaggtgatgc atcacgaatg tgccttcggg cctcgggaac
      361 tgaacgtgga tcacgatcga gacccccggt gacgtcgcag cctcgatcga aacgctcgaa
      421 cacggccacg gaccgctggc agatgatctc gcagcgttgt acgacgacga cttggagcgg
      481 cgggtcctta cgaactgggg cgacgagtgg ccagcttgcc gcatcttcac gaccatgacc
      541 aaacacaccg ccatcacgga gcggagatcg gagtcctgcg cgacctgtat cgaatcgccg
      601 ggcccttccg cgtatggatc gctcggccga tcctcccggt cttcgggcat ggcagaacgt
      661 cgcctgcttc gtcgccaccg gaccgatgat tctcgggggg ttcgttttcg ttcctggccc
      721 tcccgggcgg cttcgaagag gatgggagat gattccgcca tgcgggcacg gtggagcgac
      781 gcttggatcc ttgcggccgt tgattgggcg gctgatgctg acctcgccaa gatcatccaa
      841 gcggcagatg ccatcaacca cgccattccg acgatcgaag aagttgaaca cgcgatccaa
      901 ttccttggcg ctgccggtct ggtgcgctat tccgaccatt ccctgcagct aacggacgag
      961 ggaagacgcg tgtgtagtcg gactgaggcg caggacatct tcaccaccct tgaccgtcta
     1021 catgcgttcc tcaagaagtt cccggaggtc aacgacgcgt ctaacgccga atcggtcctc
     1081 gaccgttcca cgtatatcag cgccctggag cgctatgaga cactgatggt tgaagagtta
     1141 cctgagctgt acggccgagg aacgtcgtcc gcgaatcgag atggtgacga cggttccacg
     1201 gcgtagcctc acggcaatga gagccgatga gctggcgctg accccgtaaa cgcttggacg
     1261 ccctcggcgc gctcccatga cgaacctgct gcacgtctca tgctccccga attcgagtga
     1321 gccgaccgga tcggcgagtt ctggggcgag gtggaagggt cctagccgcc gtgatgatgc
     1381 tccgactaga caaacggaac ggaggcggtc ccgcctccgt tccgcgtatt acctgccggg
     1441 gtgacgctaa tggaggtacg gctcgtagta cgtaccgagc cgagtccgat agtcctcatc
     1501 gaactgggcc gggtcgtact cgggcgagtc cttgatctga tccttcgtca gggccacgaa
     1561 gatcttcttg tcatcgaggt cgatccggtc aaccgtccca gccgggatga cgaccttccg
     1621 accgaagatc cacggaccgg tgtcgacgac gatgtaggcg cgaccggtgt ccggtgttgc
     1681 ctcatcgacc ttcccgatag agccatctcg cgcctcaacg tcagacccca ccagggcctc
     1741 cctcgttccc gggaggctcg cctggaatgt ccacaggtcg gtatgcggca tgagtgacgc
     1801 ctcctttcgt gttgcgctca atctgtgtat acccagggcc cgaaaaggga ttcgccgaga
     1861 tcccccttgg cgctcgctgc gctgcagagg ctccggagca gttctccatc tacccaggtc
     1921 gcacctgcgg tttcgcagcc aagattcttg gatctggccg gtacgacccg gatgttcgcc
     1981 gagcaactga tcgactgcga ggaggaccgg gtgctgcggg cagtagtgtt cgggctgctt
     2041 gcgaagatgg agcggaagag cggcctaacc ccgagggtcg gttcgaatgg ctacctccgc
     2101 aggcgtcccc tgccgcttgg ccacgatagc gaatcggtcg ttgtcctcaa cgactatctc
     2161 gacgtcctga atgacatgtt cctcgatgac acagaactgg cgagggtcgt tacggatccg
     2221 ctcgtactcg tccttggtca acgcaataat ggaatcacac tcctcgtatc cgcactcgca
     2281 gacgatgtgc atgaacgagc tgcggggagc ggacccgtgg gcctgctgga tcgactcgtt
     2341 gatctcccgg acgacggcct cgttcttggc tatgcgttcc tccctaaagc ccataacgct
     2401 gctcctgttc tccttcaatg atgtcaaccc ctcgataccc ggagtcagcc cctcaaaccc
     2461 cgccacttgc tcgaatgggg atctcctggc tagaagttca gggtgcacgt cgagcctcgg
     2521 gcactgcgag gaggatcgga cgctgtgagc ggttctggtc ggcatgcagc tggaaacagc
     2581 cgagaagccc tggcgcttac gaatcggctt ctgcgctgca gggagtgggt aggagcgcac
     2641 gccggcctgt gaagccatgc cgagcgggtt cgattgtcca cggttgctgc cgtgacgtgt
     2701 tcgagacctg acaagcagga cggggctccc tgctctacca gccaacggac tgagggagct
     2761 gctcggccag cgcgcgaggg agtgcggccg ggccgcgtca gccagtagcc ggtaaggagg
     2821 tcatcatggc tcacatatgg cccaccaccg cggcggtcgg tacacgatga acgcccccgc
     2881 ctcgatcacc atcacgccag tcctcgttgc cgacctgcac gtcgggggcg agcggatgcc
     2941 ggtctacgtg cacgtcatcg accatcccga tgcgcgcgtg ctggtcgaca ccggcatgac
     3001 ggagctgcac ccggcggtgg ccgacctcga tccccgcctc cgtccactga gcgagcagga
     3061 cttcgacctc gccggcatcg acatcgtcgt caacacgcac ctgcactacg accactgcgg
     3121 cggcaaccac ctcttcgccg gcaggccgat ctacgtccag cgccgcgagc tcgaggacgc
     3181 gcttggcgag gacgactaca ccattcgcga gtgggtcgag gcgcccggcg tacgatacgt
     3241 gccggtcgac ggcgaactcg agctgcttcc cgggctcagg ctcgtcccgg cgccgggcca
//
LOCUS       DASTXO010000360         2765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1459371, whole genome shotgun sequence.
ACCESSION   DASTXO010000360 DASTXO010000000
VERSION     DASTXO010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459371"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VFU96_07595"
     CDS             complement(<1..536)
                     /locus_tag="VFU96_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113168.1"
                     /translation="MRSGTAPLTAGSVAGVALLLSEEIFPCADDVVVVADTNLNDVAT
                     AAQLAAALGGPLLHPHPQLAAELARLRPLRLYLIGDVKVVAPADSEVLRPNTGAAVEM
                     AKRELNVSAEVRLPALPDTSTVIETIGALTRRDRVVLPQTTPAVTPTTAPAAPVIDRA
                     AMIRGLAVPSGSEFAWMVP"
     gene            complement(762..1961)
                     /locus_tag="VFU96_07600"
     CDS             complement(762..1961)
                     /locus_tag="VFU96_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016872175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU5113169.1"
                     /translation="MNQVLRREMGLGGAIVTGLGSILGTGAFVAIGVASGLWGDAVLV
                     AIPIAGVVAIFNGMSSAFLAARFPVAGGTYEYGYRTLGPSWGFTAGWLFLLAKTASAA
                     AAALGVSTFLDTPWDPRISAAAAVVALTGLVLAGLRRTIAVNVVLVTVTVVAIVAFGI
                     AGLTQPGPASPIDREAIRLGLLPAITFLFVAYTGYGRIATLGEEVRRPAITIPRAVMV
                     TLVVAVCLYGLVAIAGRAIGGEYWGASGENDTTLVDLVAEPVSTVVLIGGVTAMLGAL
                     LNLILGLSRVWLAMGRRHDMPERLSKLDHRSTPVAAISISAVPVLLVVLLGDISIAWS
                     FSAFTVLLYYGITNLSAVVVDRKRWTAWLGLISCALLSFYVTPIVWVIGAGLVALGLV
                     WKAQRRD"
     gene            complement(1961..2644)
                     /locus_tag="VFU96_07605"
     CDS             complement(1961..2644)
                     /locus_tag="VFU96_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEU5113170.1"
                     /translation="MCGRFSITGDLDFYAEYFGVDEVLSERLEPNWNVAPTDPVYVIA
                     ERDGMRSLGSMPWGLVPHWAKDARSIHINARSETVATTSAFRESFSRRRCLIPADGFY
                     EWEPKDQGRTPHWVYRADGHPMAFAGIWASRLDPDTEIWNRTCSIITTGADGAVSALH
                     DRMPVSLVPSVWDAWLDRDLRDPEVVAGLLQPIDPEMIMEHPVSSRVNSVKNNGPELR
                     EKVEPNTLF"
BASE COUNT          535 a          856 c          952 g          422 t
ORIGIN      
        1 gggaccatcc aggcgaactc cgagcccgac ggaacagcga gccctctgat catcgccgcg
       61 cggtcgatca ctggcgcagc gggtgctgta gtcggcgtca cggcgggggt ggtctgggga
      121 aggacgactc ggtcgcgacg ggtcagggca ccgatggtct cgatgaccgt ggaggtatcg
      181 ggcagcgcgg ggagacgtac ctccgcggag acgttcaact cccgcttcgc catctccaca
      241 gcagcgccgg tgttcggtcg caacacctcg gagtccgcag gtgcgaccac cttcacgtcc
      301 ccgatcaaat agagccggag cggtctgagc cgcgccaact ccgcagccag ttggggatgg
      361 gggtggagca gcgggccgcc caaggccgct gcgagctggg cggcggtggc gacatcgttg
      421 agattcgtgt cggccacgac caccacatcg tctgcgcacg ggaagatctc ttccgagagg
      481 agtagggcaa caccggcgac cgatccagcc gtgagaggtg cggtgcccga cctcacgggc
      541 gtgcaatcgc gtggggcggg agccgggagg gtcgtgaccg ttgtggtagg ggaggtggtt
      601 gtcgtcacga acgttggcgt aggtcggagt tcggtggtct cgaccggggt ctcgcccacg
      661 atcgcgctgc atgcggtgag ggcgagggcg cacaggatcg agcgaatgac gtggttcatg
      721 gggaggctag gagccgaccg gcagggtaac ggtgccgggt gtcaatctcg gcgttgtgcc
      781 ttccagacga ggcccagggc gacgagccca gccccgatca cccacacgat aggggtcaca
      841 tagaacgaga ggagggcgca agaaatgagg cctaaccacg ctgtccatcg cttgcggtcg
      901 acgacgacgg ccgacaggtt ggtgatcccg tagtacagga ggacggtgaa tgccgagaag
      961 ctccaggcga tcgagatgtc acccagcaga acgacgagga ggacaggcac ggcgctgatc
     1021 gagatcgccg ccacaggcgt ggaccggtga tcgagtttgg aaagacgctc cggcatgtca
     1081 tggcggcggc ccatcgccag ccacacgcgc gacaggccga gtatcagatt gagaagggcg
     1141 ccaagcattg cggtgacgcc gccgatcagg accaccgtcg agaccggctc ggccacgaga
     1201 tcgaccaacg tcgtgtcgtt ctcgccggac gctccccaat attcaccccc gatcgctcgt
     1261 cctgcgatgg cgaccaatcc gtaaaggcac acggcgacga cgagggtcac catcaccgcc
     1321 ctggggatcg tgattgccgg tcggcgcact tcctcgccga gtgtggcgat ccggccgtag
     1381 ccggtgtagg cgacgaagag gaaggtgatt gcggggagaa ggccgaggcg aattgcttcc
     1441 ctgtcgattg gtgatgctgg gcctggctgg gtgaggccgg cgatcccgaa ggccacgatt
     1501 gctacgacgg tcacagtgac cagcaccaca ttgaccgcaa tcgtgcgtct cagaccagcc
     1561 agcaccaacc cggtcagcgc caccaccgcc gccgccgccg agattcgagg atcccagggc
     1621 gtgtcgagga atgttgagac gccgagggcc gcggcggctg ccgacgcggt cttggccagc
     1681 aggaacagcc acccggcagt gaatccccag gacggtccga gagtgcgata cccgtattcg
     1741 taggtgccac cggcgaccgg aaatcgtgcg gcgaggaacg ccgacgacat tccattgaag
     1801 atggcaacca ccccagcaat cgggatcgcc acgagcactg cgtctcccca gagcccggag
     1861 gcaacgccga tggcgacaaa ggctccagtt ccgagtatcg agcccagccc ggtgactatc
     1921 gccccgccca ggcccatctc gcggcggagg acctggttca tcagaagaga gtgttcggct
     1981 ctaccttttc ccgcagctcc ggcccgttgt tcttcaccga gttcacccgg ctcgaaaccg
     2041 ggtgctccat gatcatctct gggtcgatcg gctggaggag gcccgccacc acttcggggt
     2101 cgcgcaagtc ccgatcgagc caggcgtccc ataccgacgg cacgagggaa accggcatcc
     2161 gatcatggag agcggacact gccccgtcgg cgccggtggt gatgatggaa caggtgcgat
     2221 tccagatctc ggtatcgggg tcgagcctcg aagcccagat cccggcaaaa gccatcgggt
     2281 gaccatcggc ccggtagacc cagtgggggg tgcgtccctg atccttgggc tcccactcgt
     2341 agaagccgtc ggccgggatc aggcagcgcc tgcgggagaa ggattcccgg aaggcagagg
     2401 tcgtggcgac ggtttcggac cgggcgttga tgtggatcga gcgcgcatcc ttggcccagt
     2461 ggggaaccag cccccacggc atcgagccga gtgatctcat gccgtcgcgc tcggcgatga
     2521 catagacggg atcggtcgga gcgacgttcc aattcggctc gagccgctcg gagaggacct
     2581 catcgacacc gaagtactcg gcgtagaagt cgaggtcacc cgttatcgag aaacgaccgc
     2641 acacagcccg agattaggag tcgtggatgc caacacctcc catctcgacc cgagttccgg
     2701 ctgagtgaac ccgggttcgg tctcacactc cggaccgcag ggcgacgagg gtctcgagga
     2761 ggtcg
//
LOCUS       DASTXO010000361         2066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_242915, whole genome shotgun sequence.
ACCESSION   DASTXO010000361 DASTXO010000000
VERSION     DASTXO010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242915"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="VFU96_07610"
     CDS             complement(<1..817)
                     /locus_tag="VFU96_07610"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5113171.1"
                     /translation="MKPFPEGDGPKVAVLGGGVAGMSAAHELIERGFRVAVFEKQPEL
                     CGGKARSIPVPVGGLSGSCPDVAGAKGYLPGEHGFRFFPRFYKHVTDTMSRIPFGNGH
                     VIDNLVDTTRVQLSLYGKASIEMPARFPRDEGDIEKILQDLLSDLPAEMGLEEGEVDF
                     FVDRLWQVYSSCDARREEIYETMGWWEFVDADNKSKGYQKFLAEGLTRSLVAANAQLA
                     NAHVEGDIGLALILDLIKPGPSTDRVLNGPTNEVWLHPWLKYLQASGVEYQFDR"
     gene            975..1895
                     /locus_tag="VFU96_07615"
     CDS             975..1895
                     /locus_tag="VFU96_07615"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5113172.1"
                     /translation="MNRSKAFALVSVPVAVGMVWRRHRHTVRAVNSHPSVAPPLPGEC
                     REITTSWGSIAYRWREGDPDRPALVLVHGWGRTADSTWWPLIATSDRTLAVIDLPGHG
                     RSRLDQPFTFELAAEAVNRAIEHSGLVRPVLVAHSMGGPVLLTALRGSDPGQFSGLVA
                     MATSVYWGRPRLRAILAMAPYLLANGSPVLIHREHAELRHAPESASHIAWAYTQRPLR
                     PRLREAAAALRRFDARGWTDLELPDTTWVVAGRDRVLAPDNQRASARHFGAEMVELDV
                     EHSMVVPAHRDLIEILDTVGCSPLSAGETA"
     gene            complement(1875..>2066)
                     /locus_tag="VFU96_07620"
     CDS             complement(1875..>2066)
                     /locus_tag="VFU96_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113173.1"
                     /translation="GAGTCIALAPSAFQWRKGDFLKPELLDATTVDEDTLREVAAACP
                     TQAIVLEEVIESLSGGLTG"
BASE COUNT          337 a          666 c          664 g          399 t
ORIGIN      
        1 gacggtcgaa ctggtactcg acacccgagg cttgcagata cttcagccag gggtggagcc
       61 agacctcgtt ggtcggcccg ttgagcaccc ggtcggtgct cgggccaggt ttgatcaggt
      121 cgaggatcag ggccaagccg atatcgcctt cgacgtgagc attggccagt tgggcattcg
      181 cggctaccag ggagcgcgtg agcccttcgg cgaggaactt ctgatacccc ttcgacttgt
      241 tgtcggcgtc gacgaactcc caccagccca tggtctcgta gatctcttcc cgtcgcgcat
      301 cacagctcga atacacctgc cagagcctgt cgacgaagaa atcgacctct ccttcctcga
      361 gacccatctc cgcaggcagg tcggagagga ggtcctggag gatcttttcg atatccccct
      421 cgtcgcgggg aaagcgggcc ggcatctcga tcgaggcctt gccatagagc gagagctgca
      481 ccctcgtggt gtcgaccagg ttgtcgatca catggccgtt cccgaatggg atccggctca
      541 tggtgtcggt gacgtgtttg tagaaccggg ggaagaaccg aaagccgtgc tctccgggca
      601 ggtatccctt cgcaccggcg acgtcggggc aggacccgct cagcccgccg accggcactg
      661 ggatactccg ggccttgcct ccgcacagct cgggctgttt ctcgaagact gctacccgaa
      721 agccacgttc gatcaactcg tgggcagcac tcatcccggc gacgccacca ccgaggaccg
      781 cgaccttggg gccatcccct tcggggaatg gtttcaatgc gactggttcg attggagtgt
      841 cgatgatcgg ggcgaagtta cacgcgcgga ctttgaaggg acccggatcg gcaggtctag
      901 tccccagggg gtcgaatgcg gatggtccgt gcgctccata aacccaagcc caggaccgtg
      961 ccgattacac acggatgaac cgctccaagg cattcgccct ggtctcggtg ccggtggccg
     1021 tcgggatggt ctggcgtcgg catcgacaca cggttcgggc ggtgaacagc cacccatcgg
     1081 tggctccacc gttgcctggc gagtgtcggg aaatcaccac ctcatggggg tccattgcct
     1141 accggtggcg agagggtgac cccgatcggc ctgccctggt cctcgtgcat ggatggggga
     1201 ggaccgctga ctcgacctgg tggcccttga tcgccacctc cgatcggact ctcgcggtca
     1261 tcgaccttcc cggccacggt cggtccagac tcgatcaacc gttcacgttc gagctggccg
     1321 ccgaggcggt caaccgtgcc atcgagcatt caggtttggt ccgtcctgtg ctcgtagccc
     1381 actcgatggg cggccccgtg ctgctgactg cgctgcgggg atccgacccg gggcaattct
     1441 cgggcctggt ggcgatggcc acctccgtgt actggggacg gccccggctt cgtgccatcc
     1501 tggccatggc cccctatttg cttgctaacg gctcgccggt cttgatccat agggagcatg
     1561 ccgagttgcg tcatgccccg gaatcggctt ctcacatcgc ctgggcatac acccaacgcc
     1621 cgcttcgccc caggctccgg gaggcggctg ctgccctgcg tcgcttcgat gcgcgtggtt
     1681 ggaccgatct cgaactgccc gacaccacct gggtggttgc gggtcgagac cgggtgctgg
     1741 ccccagataa tcaacgggcg tccgcccggc attttggcgc cgagatggtc gagctggacg
     1801 ttgagcattc catggtcgtc ccggcacatc gagatctgat cgagatcctc gacacggtcg
     1861 gctgctcgcc gctgtcagcc ggtgagaccg cctgacagcg attcgatcac ttcctcgaga
     1921 acgatcgcct gggtagggca ggcagcggcc acctcccgca gagtgtcctc gtcgaccgtt
     1981 gtcgcatcga ggagctcggg tttgaggaag tcgccctttc tccactggaa ggccgagggg
     2041 gcgagggcga tgcaggtgcc ggcgcc
//
LOCUS       DASTXO010000362         3329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_575050, whole genome shotgun sequence.
ACCESSION   DASTXO010000362 DASTXO010000000
VERSION     DASTXO010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575050"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VFU96_07625"
     CDS             complement(<1..424)
                     /locus_tag="VFU96_07625"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU5113174.1"
                     /translation="MPTLRSRLWWLISLPVIRLGARIMWRLRVDRGLEFPHPPFVLAA
                     NHYSFLDPPLVGAVFGKRARFIALIDLYGNHRSLDWALDSWEAITVRRGVVPLGTVRQ
                     CLEHLESGGVVGLFPEGIRVEKFGDAGFKRGAAWLAART"
     gene            complement(442..1410)
                     /locus_tag="VFU96_07630"
     CDS             complement(442..1410)
                     /locus_tag="VFU96_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEU5113175.1"
                     /translation="MKAITVPALGGPENLVLDDLPEPEGGPGDLVVEVAAAGVNYVDV
                     YHRSGLYDITLPFVPGVEGAGTVIAVGEAVDGFVIGDQVGWADVRGSYEERHVIPASR
                     AVPIPGGLDSKIVAAALLQGLTAQYLSTDTFPLHEGHRCLIHAGAGGVGLLLTQIAKL
                     RGAEVFTTVGSEDKAELSRGAGADHVIVYTQVDFQDAVEELAGPKPLDVVYDGVGADT
                     FMKGLDLLRPRGMMVTFGNASGPVPEIAPLLLSQKGSLFLTRPSMGHYLLTTEELLTR
                     SNDVFGWIQSGELEVRIGREFPLADAADAHRALEGRQTTGKVLLLP"
     gene            complement(1407..1826)
                     /locus_tag="VFU96_07635"
     CDS             complement(1407..1826)
                     /locus_tag="VFU96_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113176.1"
                     /translation="MGDTVLVAVDVPLVGDERHKNWAKVVESVDDSRASGWAFEGPFV
                     AAGGIQDLPMGATLLVYGERGSRANPQITARVYTVNPDSTLSLEAEAKGKAWARTLRD
                     PVERCLGADSRPPDLSFISDELLALELRRRGWRVEQP"
     gene            1892..2890
                     /locus_tag="VFU96_07640"
     CDS             1892..2890
                     /locus_tag="VFU96_07640"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEU5113177.1"
                     /translation="MAKLRLFANLRELAGTSRLDVPSETVGGVIEAASEKFGPDFRRG
                     VETSRVWVNGEAASMDDTVTEHDEVVLIPPVSGGDQPTATFSAVDLMAFLPAAVAVAA
                     VLANTQGQDIWAAVLVLIVAIWALDIGSAFEARGREFASLAVVVTAAGSAMAAHVAGG
                     GGYALSLVIAVVVGLGWAVAFPQYRQVEVFSPTLMVALLAGLGTASLVLARSAHSPEA
                     SAVDVFLVSVIAAVGLSAILERINPIPLLDPFSVTALGAVLAAVAAALIWDLSVVSYL
                     LVGLGIAVALLAGRGLSSMLRMGRVVLTERPPGALVSLDGVVLAAAIYYPLLLLVL"
     gene            2956..>3329
                     /locus_tag="VFU96_07645"
     CDS             2956..>3329
                     /locus_tag="VFU96_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113178.1"
                     /translation="MRAQTTPVRVITLLTLIGLIAVLFPLAGQAVTKTQVEEACADSR
                     AQLEDYREAQAAFEVAAHEYEDAVIEVDRVERKQESVAGSVSSHTEELDQIQAGIEEQ
                     AVELYMRGGVAGPGIIFSASSV"
BASE COUNT          544 a         1147 c         1061 g          577 t
ORIGIN      
        1 cggtccgagc cgccaaccag gctgcgccac gcttgaaccc ggcatcaccg aatttctcca
       61 cccggatgcc ctccgggaaa agaccgacga cacctccgga ttcgagatgc tccaggcatt
      121 gccggaccgt tcccaggggg accacgcccc gtcgcaccgt gatggcttcc caggaatcga
      181 gggcccagtc gagcgaccgg tggttcccat acagatcgat gagagcgatg aaccgtgccc
      241 gcttcccgaa cacggccccg acgagtggag ggtcgagaaa cgaatagtgg ttggcggcga
      301 gcacgaacgg cggatgggga aactctagtc ctcgatccac ccggagcctc cacatgatcc
      361 gggcgccgag cctgatcacc ggcagcgaga tcagccacca caggcgactc cggagggtgg
      421 gcatcgacga ctcgggttgg gtcaagggag caacaggacc ttgcccgtgg tctgcctccc
      481 ctccagcgca cgatgggcgt cggcggcatc ggcgagaggg aactcgcggc cgatgcgcac
      541 ctcgagctca ccagattgga tccagccgaa aacgtcgttg ctgcgtgtca ggagctcttc
      601 ggtggtcagc aggtaatggc ccatcgacgg ccgcgtcagg aagagcgacc ctttttggga
      661 gagcagaagc ggcgcgatct cagggaccgg gcccgaggca ttgccaaacg tcaccatcat
      721 cccgcggggc cggagcagat cgagtccctt catgaacgtg tcggcgccaa ctccgtcgta
      781 cacgacgtcg agcggctttg gaccagccaa ttcctcgacg gcgtcctgga aatcgacctg
      841 cgtgtagacg atgacatgat ccgctcccgc cccgcggctc agctccgcct tgtcttcgct
      901 tcccaccgtg gtgaacactt cggccccacg cagcttggcg atctgggtga gcaggagacc
      961 gacgcccccg gccccggcgt ggatcaggca tcgatgtccc tcgtgtagcg ggaacgtgtc
     1021 ggtggacaga tattgggcgg tgagaccctg caacagtgcc gcggccacga tcttggagtc
     1081 gagaccgccc gggatcggca ccgcgcgact cgccgggatc acatgccgtt cttcgtagga
     1141 accacggacg tctgcccagc ccacctggtc gccgatgacg aacccatcga ccgcctcgcc
     1201 caccgcgatc acggtgcctg ccccttccac ccccgggacg aagggaaggg tgatgtcgta
     1261 gagcccgctg cggtggtaga cgtcgacgta gttcaccccc gccgcagcca cctcgaccac
     1321 gagatccccg ggcccgccct cgggctcggg caaatcgtcg agcaccaggt tctcgggccc
     1381 gccgagggca gggacggtta tggctttcac ggttgctcca ccctccaccc ccgtcttcgc
     1441 agctcgagtg ccagcaactc atcgctgatg aacgagaggt caggcggcct cgaatcggcc
     1501 ccgaggcagc gctcgaccgg gtcgcggagt gtgcgggccc aggccttccc cttggcctct
     1561 gcctccaggc tcagcgtgga gtcgggattg accgtgtaga cccgtgctgt gatctgagga
     1621 ttggcccgcg agccccgctc gccatagacg aggagagtgg cgcccatcgg caggtcctgg
     1681 atgccgccgg ccgccacgaa cggcccttcg aacgcccacc ccgaagccct cgagtcgtcc
     1741 accgactcga ccaccttcgc ccagttcttg tgacgctcat ctcctaccag cggcacatcg
     1801 accgccacca agaccgtgtc gcccataggt cgagagtagg cgagggcagc ccggactcgc
     1861 gaggtgaagt cggtacgctc taactcgaca catggccaag ctccgcctct tcgcaaacct
     1921 ccgggaactg gccggcacat cccggctcga tgtcccgtcc gagaccgtcg gaggggtgat
     1981 cgaggccgca tcggagaagt tcgggcccga tttccggcgc ggcgtggaga cttcacgggt
     2041 ctgggtcaac ggtgaggccg cctcgatgga cgacaccgtg accgagcacg acgaggtggt
     2101 gctgatacca ccggtctcgg gtggggatca gccgaccgcc acgttctccg cagtcgacct
     2161 gatggcattc ctgcccgcgg cggtggccgt ggccgcggtc ctggccaaca cccaggggca
     2221 agacatctgg gccgccgtcc tcgttctgat cgtcgccatc tgggcgctcg acatcggctc
     2281 ggcgttcgag gcgagggggc gcgagttcgc gtcactcgcc gtcgtggtca cggcggcggg
     2341 gtctgccatg gcggctcacg tcgcgggtgg aggcgggtat gccctctccc tggtgatcgc
     2401 agtcgtcgtg ggcctgggtt gggcggtcgc cttcccccaa taccggcaag tagaagtatt
     2461 cagccccacc ctgatggtcg ctctcctcgc cgggctgggc acggcttctc tggtgcttgc
     2521 ccgctccgca cattcccccg aggccagcgc cgtcgacgtc ttcctcgtgt ccgtcatcgc
     2581 cgcggtcggc ctcagcgcca tcctggaacg gatcaacccg atccccctcc tcgatccgtt
     2641 ctcggtgacc gctctgggag ccgtcctggc cgctgtggcc gccgctctga tctgggatct
     2701 ctctgtcgtc tcgtatcttc tggtcggtct gggtatcgcc gtcgcactgc tggcgggccg
     2761 aggtctgtcg tcgatgctgc gcatggggag ggtcgtcctg accgaacgac ccccgggagc
     2821 gttggtcagc ctggacgggg tcgttttggc ggccgcgatc tactacccgc tgctgctctt
     2881 ggtcctctga cactcgtttg caaataggcg gttccgacta gagcagatcg ccgtctcggg
     2941 ttagccttcc cgtcgatgcg tgcccagacg acgcccgtcc gcgtcatcac cctcctcacc
     3001 ctcatcggcc tcatcgctgt cctattcccc cttgccggcc aggccgtgac caaaacccag
     3061 gtcgaggagg cctgtgccga ttctcgtgct caactcgagg actaccgcga ggcccaggcg
     3121 gccttcgagg tcgccgccca cgagtacgaa gacgccgtga tcgaagtcga tcgtgtcgag
     3181 cggaaacaag aatccgtcgc cggctcggtg tccagtcaca cggaggaact ggatcagatc
     3241 caggccggga tcgaggagca ggcggtagag ctctacatgc gaggcggtgt ggccggcccc
     3301 gggatcatct tctccgcgtc ctcggtcga
//
LOCUS       DASTXO010000363         2478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_206273, whole genome shotgun sequence.
ACCESSION   DASTXO010000363 DASTXO010000000
VERSION     DASTXO010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206273"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /locus_tag="VFU96_07650"
     CDS             <1..1251
                     /locus_tag="VFU96_07650"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5113179.1"
                     /translation="VPLLVNEALTSEGDGPSTPGMARYMEARLLEVGDLGRQILTAAA
                     ALNGICDATLLRETSGRSEEEVVEAVEELVAAGLLREIPESDGLSFTLDALERVTYES
                     TSLVRRRLLHRRAARALSERPYARSDARLAAAVAGQYRSAGDTEAAAWYHLAGDLARS
                     VFANDSAVQFYETALALDDPDVESARLALGELAMAAGDYDRARRELTLAASHAGPGSI
                     GVIEHRTGEVERLLGRFEDAERHFQRSIENHPAPAAVLADWALLGYRTGDVAKATTMA
                     RRARDAAEKENDPAQMSRVRNILAVVTPDHGEALEHVEEAIRLAGDDDLLRMAALNNK
                     ALLLSENGDVGRSTALVEEAIGIAERTGHRHREAALRNHMADLHHRAGRDDDARQSLT
                     TAVSLFADIDAGDLEPELWLLSRW"
     gene            1323..1595
                     /locus_tag="VFU96_07655"
     CDS             1323..1595
                     /locus_tag="VFU96_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113180.1"
                     /translation="MTVSTITTLDEWAAHPLGKAARIGLAVLGLASAVLVLMVATMIS
                     QAAWLLVLLGVALAATSVRAASKPSVIRLTTLGAVMVSVLYVGQTF"
     gene            1922..>2478
                     /locus_tag="VFU96_07660"
     CDS             1922..>2478
                     /locus_tag="VFU96_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113181.1"
                     /translation="MSSFTKRGSADLEATLRRMVVVFRFLGLIWMLILVAVTVLNDPP
                     PVMWIVWAAVGLAVFWTFLTWLVAKTRPEFMAGWWWFALDAVAMLAIGAASVASGAEE
                     LFHGGLPLSLVFTGALLGGLPGSLIAALLLGVEQFLVHVIADLGAVRAAGSVIFFVVG
                     AIVGWTFDKLRDYDLARQAAQDELAA"
BASE COUNT          398 a          823 c          802 g          455 t
ORIGIN      
        1 gtgcccctcc tggtcaacga ggctctcacc tcagaagggg acggcccatc gacccccgga
       61 atggctcgct acatggaggc gcgactgctg gaggtcggtg atctgggtcg acagatcctc
      121 accgccgccg cggcgctcaa cggcatctgc gacgcgaccc ttcttcggga gacgagcgga
      181 cgcagtgaag aggaggtggt ggaagcggtc gaggagctgg tcgccgcggg tctgctgcgg
      241 gagatccccg aatccgatgg gctcagcttc accctcgatg ctctcgaacg agtcacctac
      301 gagtcgacgt cgctggtaag gcgccgactg ctccatcgcc gagctgcccg agcactctcg
      361 gagcggccat acgctcgatc cgacgcccgg ctcgccgccg cggtcgccgg ccagtatcga
      421 tcggccggcg ataccgaagc cgcagcctgg tatcacctgg ccggcgacct ggcccgttcc
      481 gttttcgcca atgactccgc agtccagttc tacgagaccg ctcttgcgct ggatgacccc
      541 gacgtcgaaa gtgcccgcct cgcgctgggt gagctcgcca tggcggccgg cgactatgac
      601 cgcgcccgac gtgagctgac cctggcggcg tcgcacgcgg gacccggatc gataggggtc
      661 atcgagcatc ggaccggcga ggtagagcgt ttgctcggcc gcttcgaaga cgcggaacgg
      721 cattttcagc gatccatcga gaaccatccg gccccggccg ccgtgctcgc cgattgggcg
      781 ctcctcggat accgcaccgg ggacgtggcg aaggcgacga ccatggcccg ccgggcacgg
      841 gatgcggccg agaaggagaa cgacccggcc cagatgtccc gcgtccgaaa catcctcgcc
      901 gtggtcaccc cggatcatgg agaggcactg gagcacgtcg aggaggcgat tcgcctcgcc
      961 ggcgacgacg atctactccg gatggctgcg ctgaacaaca aggcgctgtt gctctcggag
     1021 aacggagatg tcgggcgttc gaccgctctg gtcgaagagg cgatcggcat cgcagagcgc
     1081 accgggcacc gtcatcgaga ggctgctcta cggaaccaca tggccgatct ccatcatcgc
     1141 gccgggcgtg acgacgatgc gcgccaatcc ctcacaaccg cggtgtcact cttcgctgac
     1201 atcgacgccg gcgacctgga accggagttg tggctcctct cccgatggtg agatttcggt
     1261 ccgatcggcc caggccggcg gactcaagaa atcgggggcc tctgccgaca tttcccggca
     1321 cgatgacagt ctccaccatc accaccctcg acgagtgggc agcgcatccg ttggggaagg
     1381 cggcccggat cgggctcgcc gtcctgggtc tggcatcggc ggtacttgta ctcatggtgg
     1441 caacgatgat cagccaggcc gcctggctcc tcgtgctcct gggcgtcgcg ttggccgcta
     1501 cctcggtgcg ggccgcctcg aagccgagcg tcatccgact caccacactc ggcgcggtca
     1561 tggtctcggt cctctacgtg ggacaaacct tctagagctc ttagcgaagg gccacaacgg
     1621 ccccggcgcc ctgcgcacac ggccaccggc taacggtggc cgttgtgttt tcaggactgc
     1681 cccgtgtcct tggcctgtat gagtggatcg gatgggggtg atcccctatt gggttttcca
     1741 cgggaatcgt catgatcagt ggtgcgggga cttcagggca taactccgtc cctcgaagcg
     1801 acaatgggga ccatcggggg accgcggaag cggtccccct ttgaacgcga aaaaggacgc
     1861 catccaactc tcacctctcc caaggaccaa cccgccaccc accccgctag tttcgggtga
     1921 aatgagcagc ttcaccaagc gaggcagcgc cgatctcgag gcaaccctcc gacggatggt
     1981 cgtggtcttc cgtttcctcg gcctcatctg gatgctgatc ctggttgcgg tgaccgtctt
     2041 gaatgacccg ccccccgtca tgtggatcgt gtgggcggca gtgggtctcg ccgtcttctg
     2101 gaccttcttg acctggctcg tcgccaagac ccggcccgag ttcatggccg gttggtggtg
     2161 gtttgccttg gacgcggtgg ccatgctcgc tatcggagcg gcatccgtcg catcgggagc
     2221 cgaggaactt ttccacggtg gcctgcctct gtctctcgtc ttcaccgggg ctttgctggg
     2281 tggcctcccc gggtccttga tcgctgcact cctgctcgga gtcgagcagt ttctcgtcca
     2341 cgtgatcgcc gatctcggag cggtgcgggc tgcgggcagc gtgatcttct tcgtggtcgg
     2401 cgcaatcgtc ggttggacct tcgacaagct tcgtgactat gacctcgccc ggcaggcggc
     2461 tcaggacgag cttgccgc
//
LOCUS       DASTXO010000364         2007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1311884, whole genome shotgun sequence.
ACCESSION   DASTXO010000364 DASTXO010000000
VERSION     DASTXO010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311884"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="VFU96_07665"
     CDS             <1..939
                     /locus_tag="VFU96_07665"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5113182.1"
                     /translation="NPLEVARAIVVGALGNPFNIGQTVVITGILILTALAAAIPFTAR
                     LWNVGGEGQMTVGAIAAGVLGIVLPATWSRWLLVPVVLIGSMVAGSLYGAIPGWLKAR
                     FDASEIVTSLMLNFVAASLAAWVIFEVYPEGFVQRTKAIHVNAELPRPIEGSLIDIGI
                     ILAVASAVIAWFVMRHTRLGFSIRAIGANNPASRLSGIRTDRVTVLTFLMAGAFAGLA
                     GALIVQGRDHSLSQDFSANLGFLGIGVALVARLHPLWIIGSAAFFAILRVGSNSLQAG
                     AGLSPVVGEIVIATFVILLMVTKVIKFRYPESVDVH"
     gene            929..>2007
                     /locus_tag="VFU96_07670"
     CDS             929..>2007
                     /locus_tag="VFU96_07670"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5113183.1"
                     /translation="MFTEALVLAFFVIGIQLMVPIVMAALGEAVAEKSGVLNVGIEGM
                     MLIGAFATAYMGITRRSAGLGILAAILAGCVFGALLAFLYVNRGTDQIVTGLMFNIFA
                     LGLTGTLHSLYLAGQNGETLTGIVLPGIGSIPWIGEILNKQSLMLYLAIALAFGVWYL
                     LNKTWYGLYARAAGERPLAAESGGLNVLRLRYPAVIVASAMAALGGSALVLATSGGFV
                     PGMTAGRGFIALGVVVLAKWRPLWIMIYALGFGLTQGLQFLAGQVPALQSVPTQFWVA
                     LPYLVTVIAVVFAPGSTYPAAVGIPYRRAGSRPSAPRVGRRPLGRRSFDVDKVGRVLL
                     HGGRSNMGEGDARDKVEVGSAMAVAG"
BASE COUNT          291 a          635 c          651 g          430 t
ORIGIN      
        1 aatcctctcg aagtggcgag ggcgatcgtc gtcggcgcct tgggcaatcc gttcaacatc
       61 ggtcagacgg tggtgatcac cgggatcctc atcctgaccg cattggcggc ggcgattccg
      121 tttactgccc gtctctggaa cgtgggtggt gaggggcaga tgacggtcgg agcaatcgcc
      181 gccggcgtgc tcggcattgt ccttcccgcc acttggtcgc ggtggctgct ggtgccggtg
      241 gtgctcatcg gatcgatggt ggctggttcg ctgtacggcg cgatccctgg ctggctgaaa
      301 gcccgcttcg acgccagcga gatcgttacc agcctcatgc tcaacttcgt cgccgccagc
      361 ctggcggcat gggtcatctt cgaggtctac cccgaggggt tcgtgcagcg gaccaaggcg
      421 atacacgtga acgcagagct cccccggccg atcgaaggct ccctgatcga catcgggatc
      481 atccttgccg tcgccagcgc ggtcatcgcc tggtttgtga tgaggcacac cagactgggc
      541 ttctcgatcc gggcgatcgg ggccaacaac ccggcgtcac ggttgtccgg gatcaggacc
      601 gaccgggtca ctgtcctcac cttcctgatg gcaggcgcct tcgcaggcct cgccggggct
      661 ctgattgtcc agggtcgcga tcactctctc tcgcaggact tctccgccaa cctgggtttc
      721 ctcggcatcg gcgtggcgct cgtcgcccgg ctgcatccgc tgtggatcat cggctcggcc
      781 gcctttttcg ccattctccg tgttggctcg aacagccttc aggccggcgc cgggctgtca
      841 ccggtggtgg gcgagatcgt gatcgccacg ttcgtgatcc tcttgatggt gaccaaggtc
      901 atcaagttca gatatccgga gagcgtcgat gttcactgag gcactggtcc tggcgttctt
      961 cgtgatcggg atccaattga tggtgccgat cgtgatggca gcgttgggtg aggccgtcgc
     1021 cgagaagtcg ggggtgctca acgtcggcat cgagggcatg atgctgatcg gcgcattcgc
     1081 caccgcctac atggggatca cgcgccggag cgcagggctc gggatcctcg ccgcaatcct
     1141 ggccggatgt gtgttcggcg ccttgctcgc cttcctctac gtgaaccggg gcaccgacca
     1201 gatcgtcacc ggcttgatgt tcaacatctt cgccctcggc ctcaccggaa cgcttcactc
     1261 gctctacctg gccggccaga acggggagac gttgaccggg atcgtcctcc ccggcatcgg
     1321 atcgatcccc tggatcggcg agatcctcaa caagcagagc ctcatgctgt acctcgcgat
     1381 agctttggcc ttcggggtct ggtacctgct caacaagacc tggtacggcc tgtacgcgag
     1441 ggcggcaggt gaacggcccc tcgccgcgga gtcgggtggg ctgaacgtcc tgcgactccg
     1501 ctacccggca gtgatcgtcg cctcggcaat ggccgcgctc ggcggctcgg ccttggtcct
     1561 cgccacgtcc ggcggcttcg taccgggcat gaccgccggt agggggttca tcgccctggg
     1621 ggtggttgtg ctcgccaagt ggcggcctct ttggatcatg atctatgccc ttgggttcgg
     1681 tttgacccag ggtcttcagt ttctggctgg tcaggtgccc gccctgcaat cggtacccac
     1741 acagttttgg gtggctcttc cctatctcgt cacggtgatc gctgtcgtct tcgctcccgg
     1801 ctcgacctat ccggccgccg tggggatccc gtaccgccgc gccggttcta gaccctccgc
     1861 gcctcgagtt ggcagacgtc ccctcggacg ccgctcgttt gatgtagata aagtaggtcg
     1921 agtgctgctg cacggcggca gatccaacat gggagaagga gatgcgagag acaaagtcga
     1981 ggtgggctcc gcgatggctg ttgctgg
//
LOCUS       DASTXO010000365         4550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_796013, whole genome shotgun sequence.
ACCESSION   DASTXO010000365 DASTXO010000000
VERSION     DASTXO010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796013"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VFU96_07675"
     CDS             <1..748
                     /locus_tag="VFU96_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113184.1"
                     /translation="AQGSPFYFSWSPDSSQVAAHVQSQFFGTIALDGEGADLGATDPG
                     YQAPHWIPAGILHLSADGLELRQVDGEARLLATVPGPIAFVANRQGTSVAVQSFVEEE
                     TPPGVNAAVTETPAIPSNTVVVIDIESGEMSEVIASTSIGLFWSPDGESLLVLNPDEG
                     GAPELTTLVWRDGSTTELSPMSPDPAFVADVLRFFDQYGQSLQLWSPDSAAFALPGAI
                     AGDSGIWVHLVAGGDPVNVHEGSWVSWSSM"
     gene            817..2322
                     /locus_tag="VFU96_07680"
     CDS             817..2322
                     /locus_tag="VFU96_07680"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEU5113185.1"
                     /translation="MAVFTGLPDFDPVPSLRVPASVTVATKAPSERPVIGYLVAAEGD
                     IPDRIGLNRSALEAAGFDGEVGKTIVLPAADGPMTVLVGVGEVSALDADRLRDAGAAY
                     ARATQQQGDLALDLGEIGAVAPDVAGQALVEGALLARYRYDVLKEKPTVKPVSDLALV
                     VDGRRSAAVRRGAARGVITAEATNIARDLANTPPALLTAIDITEVAIRLGKERGLDVE
                     TFDKRALIDLGCGGLLGVNAGSAGEPRMIRLSYRPARPRGHLTLVGKGIMYDAGGMAL
                     KPADDVHATMKNDMSGAGAILAAMAQLEALGCKTAVTGYLMCTDNRPSDTSIAMGDVL
                     TVYGGKTVEVMNTDAEGRLVMADALVLATEDETDAIVDVATLTGAAMRALGTQVAGLI
                     GNNAGVVAQVEAAADATGESVWELPLERRYRGDLNSDIADIKNLGGANAGAITAALFL
                     EEFVAGKPWAHIDIAGTAQNDRDTSWRPPGCTGFGARLLVELALNFKRPTD"
     gene            2337..3992
                     /locus_tag="VFU96_07685"
     CDS             2337..3992
                     /locus_tag="VFU96_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HEU5113186.1"
                     /translation="MTSGTAVVKKSFSERMLDGVERVGNKVPHPVLMFLYLILFIIVL
                     SHILYLLDVSATEEITVAVPTEEQIDEHGGLGGSDEPYPETPILGNYDPEYEIVTETI
                     PVVSLLTLDGIRFIFTSFVDNFASFTVVAVVFVAMMGVGVAEHAGMMGALIRKLVNVA
                     PRQFIAFILIFVGVLSSIASDAGYLILIPLSAAAFMTLGRHPLAGMAAAFAGVGAIFA
                     VNLLITPVDSMITEISNEAIALAGGEPLTVVANYFFSVVSSVVIAIVAWWVTVRMVEP
                     RLGNYDVSEANADYGQDAAVDEEGEARGLRYALYGALAVIAVVALFTIPSWGPLRHPE
                     TGEVIGNTPFMDSLIFIITLIFLVAGICYGRGAKTLSGSNDVIAGVTKTFAGLSGLVF
                     MLLMISQFIAYFNYTNMPRVAAIEMAHLLEAAPFGPLPLLVAFILVIILLDFIIPGVV
                     PKWAIFAPVFIPIFLRLDVAPQTLLAAYRVGDSPVNVLTPLMVYLPFIVTVAQRYKKD
                     AGLGTVISLMIPYAVAITVVWIILFVAWFALGLPLGPGYPVST"
     gene            3996..>4550
                     /locus_tag="VFU96_07690"
     CDS             3996..>4550
                     /locus_tag="VFU96_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019744656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEU5113187.1"
                     /translation="MSDAAIVYLVLVAVVALFIWNKLPVEVVAVGSALTLAATGVLDL
                     DQALAGFGDTTVLFIAALFIVSEGIDSTGVTTWAGQQLINRAGTSRTRLVVFMMLLVA
                     LLTAVISVNGAVAALLPMVVVIALRVGRAPSSLLMPLAFGAHAGSLLALTGTPVHILV
                     SDAAIEAGEAGFGYFEFGLVGVPVV"
BASE COUNT          742 a         1461 c         1449 g          898 t
ORIGIN      
        1 ggcgcaggga tccccgttct acttctcgtg gagccccgac agcagccagg ttgccgccca
       61 tgttcagtcg cagttcttcg ggaccattgc tctcgatggc gaaggagccg atctcggcgc
      121 gaccgacccg gggtaccaag ccccgcactg gatccctgcc gggatcctcc atctgtccgc
      181 cgacggtctc gagctgcgcc aggtcgatgg cgaggctcgt ttgttggcca cggtccccgg
      241 acccatcgca ttcgtggcca accgccaggg caccagcgtg gccgtccagt cattcgtcga
      301 ggaagagacc cctcccggtg tcaacgcggc cgtcaccgag acaccggcga tcccttccaa
      361 caccgtcgtg gtgatagaca tcgaaagcgg ggagatgtcc gaggtgatcg cctccacctc
      421 catcgggctt ttctggagcc ctgacgggga gtcgctgctc gtcctgaatc ctgacgaggg
      481 cggcgctcct gagctgacaa ccctggtctg gcgcgacggc agcaccaccg agctctcgcc
      541 catgtccccg gatccggcgt ttgtcgccga cgtgcttcgt ttcttcgatc agtacggcca
      601 atcactgcaa ctttggtcac ctgactcggc ggcgttcgcc ctgcccgggg ccatcgcggg
      661 cgattccggg atctgggtcc acctcgtcgc cggtggtgac ccggtcaacg ttcacgaagg
      721 atcttgggtt tcctggtcca gcatgtgatt cccaagcggc gaacttgata gtttcggcgg
      781 ccgcgtctcc atccgcgtct ggaaaggtcg ttcttagtgg ctgtcttcac cggtctcccc
      841 gacttcgacc cggtcccctc cctgcgtgtc ccggcttcgg tgacagtggc gaccaaggct
      901 ccttccgaaa ggccggtgat tggctatctg gtcgccgcgg aaggtgatat ccccgatcgc
      961 atcggattga accgttccgc gctcgaagcg gccggcttcg acggcgaggt cggcaagaca
     1021 attgtcctac cggcagccga cgggccgatg acggttctgg tcggtgtcgg tgaggtgtct
     1081 gcgctggatg ccgacaggtt gcgtgacgct ggagccgctt atgcccgcgc cactcagcag
     1141 caaggagatc ttgccttgga cctgggcgag atcggcgcag tcgcacccga cgtcgccggc
     1201 caagccttgg tggaaggagc actgctcgct cgctatcgat acgacgtctt gaaagagaag
     1261 cccaccgtca agccggtcag cgatctggcc ctcgtggtgg acggaagacg atcggcggca
     1321 gtcagacggg gcgcggcccg tggggtgatc accgccgagg cgacgaacat cgcccgtgac
     1381 ctggccaaca ccccgccagc ccttctcacc gccatcgata tcaccgaggt ggcgatcagg
     1441 ctgggaaagg aacgcgggct cgacgtggag accttcgaca agagggcatt gatcgacctg
     1501 ggctgcggcg gcctgctcgg ggtcaacgcg ggcagcgccg gggagccgag aatgatcagg
     1561 ctgagctacc ggcccgcccg tccgagaggg catctgactc tcgtgggcaa gggaatcatg
     1621 tacgacgccg ggggaatggc gctgaagcca gccgacgacg tgcacgcgac gatgaagaac
     1681 gacatgtcgg gcgctggtgc catcttggcc gcaatggccc agctcgaggc gctcggctgc
     1741 aagaccgcgg tgaccgggta tctgatgtgc accgacaatc ggccctcgga cacctccatc
     1801 gccatgggtg atgtgctgac ggtgtacggc ggcaagaccg tcgaggtgat gaacaccgac
     1861 gccgagggac ggctggtgat ggccgatgcc ctcgtgctgg ccaccgagga cgagaccgat
     1921 gccatcgtgg acgtcgcgac gctgacgggg gcggcgatgc gagccctcgg aactcaggtg
     1981 gccggcctga tcggcaacaa cgccggtgtt gtcgcccagg tagaggcggc tgccgatgcc
     2041 accggggagt cggtttggga gctgccgctc gagcgccgtt atcggggcga tctcaactcc
     2101 gacattgccg acatcaagaa cctgggcggg gccaacgccg gcgccatcac cgccgctctg
     2161 ttcctcgaag agttcgtcgc cggcaagccg tgggcccaca tcgatatcgc gggcaccgcc
     2221 cagaacgacc gggacacatc ctggcgccca ccaggctgca ccgggtttgg agcccggctc
     2281 ctcgtcgagc tcgccttgaa cttcaaacga cccacggact gaaagggggc tcagatatga
     2341 ccagcggcac agccgtcgtg aagaaatcct tctcggagag gatgctcgac ggtgtcgaac
     2401 gtgtggggaa caaggtgccc catcccgtct tgatgttcct ctatctcatc ctgttcatca
     2461 tcgtcctcag ccacatcctc tatttgttgg acgtcagcgc caccgaggag atcactgtcg
     2521 cggtgcccac tgaagagcag atcgatgagc acgggggtct cggcgggtcc gacgagccat
     2581 accccgagac gcccatcctg ggcaactacg accccgagta cgagattgtg accgagacca
     2641 tcccggtggt gagtctcctc accctcgacg gcatccggtt catcttcacc tcgttcgtgg
     2701 acaacttcgc cagctttact gtggtggcgg tggtcttcgt ggccatgatg ggtgtcggcg
     2761 tggccgagca cgccggaatg atgggggcgc tgatacgcaa gctggtgaac gtggcaccgc
     2821 gtcagttcat cgccttcatc ctgatcttcg tgggagtcct ctccagcatc gcctcagacg
     2881 ccggctatct gatcctgatc ccactgtcgg ctgccgcctt catgactctg gggagacacc
     2941 ctttggccgg gatggcggca gccttcgccg gagtgggagc gatctttgcc gtcaacctcc
     3001 tgatcacacc tgtcgacagc atgatcaccg agatcagcaa cgaggcgatc gccctcgccg
     3061 gcggggagcc gctgacggtg gtcgccaatt acttcttctc ggtggtatcg agcgtcgtca
     3121 tcgccatcgt tgcctggtgg gtgacggtgc ggatggtcga gccgagactc ggcaactatg
     3181 acgtgtcgga ggccaacgcc gactacgggc aggacgccgc agttgacgag gagggtgaag
     3241 ctcggggcct gcgttacgcg ctttacggtg cgcttgctgt catcgcggta gtggcgctgt
     3301 tcacgatccc ctcgtggggc cccctgcgcc atcccgaaac gggagaggtg atcggcaaca
     3361 ccccgttcat ggacagcctg atcttcatca tcaccctgat tttcctcgtc gcggggatct
     3421 gctacgggag aggggccaag accctttccg gcagcaacga cgtcatagcc ggggtgacca
     3481 agacattcgc cggcttgtcc ggcctggtgt tcatgctgtt gatgatctcc cagttcatcg
     3541 cttacttcaa ctacaccaac atgccccggg tcgccgccat cgaaatggcc cacctgctgg
     3601 aagcggcgcc attcggcccg ctgccgctgc tggtggcgtt catcctggtg atcatcctcc
     3661 tcgatttcat cattcccgga gtggtgccga agtgggcgat cttcgcccca gtgttcatcc
     3721 ccatattcct caggctcgac gtcgcgcccc agaccctgct cgccgcttac cgcgtcggcg
     3781 attccccggt gaatgtgctg accccattga tggtgtatct gcccttcatc gtcaccgtcg
     3841 cccagcgcta caagaaagac gccggattgg gcacggtgat ctcgttgatg atcccgtatg
     3901 ccgtagcgat cacggtcgtc tggatcatcc tgttcgtcgc ctggttcgct ctcggcctac
     3961 cgctaggtcc ggggtaccca gtctcgacct agatcatgtc ggacgccgcc atcgtctatc
     4021 tggtgctggt ggcggtcgtc gcccttttca tctggaacaa gctgccggtc gaggtggtgg
     4081 ccgtcgggtc ggccctcacc ctggccgcga ccggagtcct cgatctggat caggcgctgg
     4141 ccgggttcgg tgacaccacc gtgctcttca tcgccgccct gttcatcgtt agcgagggaa
     4201 tcgactcgac cggggtcacc acgtgggcgg gtcaacagct gatcaaccgg gctgggacca
     4261 gcaggacgag gctcgtcgtc ttcatgatgc tgctcgttgc cttgttgacc gccgtgatca
     4321 gcgtcaatgg ggcggtggcg gcgttgttgc ccatggtcgt ggtgattgct ctacgagtcg
     4381 gccgggcacc gtccagcctg ctgatgccgc tggcgtttgg ggcccatgcc gggtcactgc
     4441 tcgcgctgac cgggactccg gttcacatcc tcgtctccga cgccgctatc gaggctggtg
     4501 aggcgggatt cggctacttc gagtttggtc tggtcggcgt tccggtcgtg
//
LOCUS       DASTXO010000366         3235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_796015, whole genome shotgun sequence.
ACCESSION   DASTXO010000366 DASTXO010000000
VERSION     DASTXO010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796015"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VFU96_07695"
     CDS             complement(<1..300)
                     /locus_tag="VFU96_07695"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEU5113188.1"
                     /translation="MTEVVVDGGPLTPGQVVEVAYRRARAIPGPDLSDRMEPARAVVD
                     AAVASGDVVYGITTGFGALASTRIDPDEAETLQYNLIRSHASGVGPPLPDVLVRAI"
     gene            366..794
                     /locus_tag="VFU96_07700"
     CDS             366..794
                     /locus_tag="VFU96_07700"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU5113189.1"
                     /translation="MTDLTSLLGEPGASVAVIGATDHSGKYGGIIYRDLKRRGLRVFA
                     VNPYRESVDGDPCWKSVLDLPETPTIADFVIPARRGLDVVDDCQQAGILNIWLQPGAE
                     SPELMEKLDAGPFNWLAHACIMVRAGLMTGGRRPEQGKVS"
     gene            complement(802..1947)
                     /locus_tag="VFU96_07705"
     CDS             complement(802..1947)
                     /locus_tag="VFU96_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5113190.1"
                     /translation="MTPNTSDRLDFSPRVTRPIVEECERLGHALIEIAETGSPGRVNR
                     QLVKALADQGLFDRLFTEQTVTATDLCAIRQGLARVSTAAETAFAVQGLGAIPIHLSG
                     NHGLRDGVMPRIRSGDTVAAFALTEPGAGSDAAALSTLAEPDGDGYRVSGEKSYISNA
                     PDADIYTVFARTTPGAGSRGITAFVVDREMPGVSGEPIPMLSPHPLGRIHLDGVFVPS
                     SNVLGEVDHGFRVAMQTLDLFRPSVGAFALGMAEAALRIATGHAMTRTAFGTPISDFQ
                     GVSHQLANVRLDIEAAELLVYSAAAAHDGGETETLTGKAAMAKLHATETAQRAVDTAI
                     QVLGARGLEADSTLAHLYLEVRAPRIYEGTSEIQRNIIARELFNGRI"
     gene            2004..2945
                     /locus_tag="VFU96_07710"
     CDS             2004..2945
                     /locus_tag="VFU96_07710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEU5113191.1"
                     /translation="MGNMHAVRIHGYGGTEALAYEEAPRPLPQASEVLIRVHATAVNP
                     FDVALRSGYLVGYFNHTLPLVLGTDVAGVVEEVGAGVDGFSPGDSVYARSGVTRDGAY
                     AEYVVVPAADVAFKPQTLDFVHSAALPHVTLTAWQALFEAADVAEGQTVLIHAAAGGV
                     GHVAVQLAKLRGATVIGTASENIDFLGELEVDQAINYATTPFEDVVSDVDVVLDTVGG
                     DTQERSWSVLKPGGILVSSVQPPSEDEAQAHGVRQHFIFSVPPIGEVLTQVAAMADAG
                     DVTPKVSTILPLTDIRKAHELIETGHTRGKIGLQVVP"
     gene            3005..>3235
                     /locus_tag="VFU96_07715"
     CDS             3005..>3235
                     /locus_tag="VFU96_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003860 - 3-hydroxyisobutyryl-CoA
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEU5113192.1"
                     /translation="MSPFRASAGLTDSWEHFGFEVAHGVGTVTFDRPDKLNALTFDVY
                     ADLRDLLGEIEHRDDIRVLVITGTGRGFCSGGD"
BASE COUNT          556 a         1039 c         1044 g          596 t
ORIGIN      
        1 gatcgcccgg acgaggacgt ccggcaaagg cggcccgaca ccggacgcgt gggaccggat
       61 caggttgtac tggagggtct ccgcctcgtc gggatcgatg cgcgtcgagg cgagggcgcc
      121 gaatccggtg gtgatcccgt agacgacgtc gcctgatgcg accgctgcgt cgaccacggc
      181 gcgggccggc tccatccggt cggagagatc tggcccgggg atcgcacgtg cccggcgata
      241 ggcgacctcg accacctggc caggagttag tggcccgcca tcgacgacaa cctcggtcac
      301 gacgaggacg gtaccgggtt gattcccgac aagccgggcg aggccgatac cgtgccgggc
      361 acatgatgac cgacctcacc tccctcctgg gcgagccagg cgcctcggtt gcggtgatag
      421 gggcgaccga ccattccggc aagtacggcg ggatcatcta tcgggacctc aagcgaaggg
      481 ggctccgcgt gttcgcggtc aatccctacc gggagagcgt cgatggtgac ccatgctgga
      541 agtccgtcct cgatctgccc gagacgccca ccatcgccga cttcgtgatc ccggcgcgtc
      601 gtgggctgga cgtcgtcgac gattgccagc aggccggcat tctcaacatc tggcttcagc
      661 ccggagccga gtccccggag ttgatggaga aactcgatgc cggcccgttc aactggctgg
      721 cacacgcctg catcatggtc cgggcaggtt tgatgaccgg cggccgtcgc cctgagcaag
      781 gcaaagtctc gtgaccttgt ctcagatcct cccgttgaac agctcccgcg cgatgatgtt
      841 gcgctggatc tccgaggtgc cttcgtagat ccggggggcg cgaacctcga gatatagatg
      901 ggccaatgtg gagtcggcct cgagacctct agccccgagc acctggatcg cggtgtctac
      961 cgcacgttgg gcggtctcgg tcgcatggag cttggccatc gcggccttcc cggtcagcgt
     1021 ctcggtctca ccaccgtcgt gggcggctgc cgccgaatac accagaagct cggcggcttc
     1081 gatgtcgaga cggacattgg ccagttgatg agacactccc tggaagtcgg agatcggtgt
     1141 cccgaatgcc gtgcgcgtca tggcatgccc ggtggcgatg cgcagcgccg cctctgccat
     1201 ccccagggca aacgccccga cgctgggtcg gaagagatcc aatgtctgca tggcgactct
     1261 gaacccgtga tcgacttcgc ccagcacgtt tgagctcggc acaaacaccc cgtccaggtg
     1321 aatccggcct agcggatgcg gcgagagcat tgggatcggc tccccgctga cgccaggcat
     1381 ctccctgtcc actacgaagg cggtgatgcc acgggatccg gcgccgggtg tggtccgagc
     1441 gaacactgtg tagatgtcgg cgtccggcgc gttggagatg taggacttct ccccgctgac
     1501 ccggtaccca tccccatccg gctcggcaag tgtggagagg gcggctgcat ccgaccccgc
     1561 ccccggctcg gtcaaggcga aggcggcgac ggtgtcaccg ctccgaattc ggggcatgac
     1621 gccgtccctc aacccatggt tcccggagag atgaattggg atcgccccca gaccctgtac
     1681 tgcgaacgcc gtctctgccg cggtggacac ccgggccaac ccttgacgaa tcgcgcacag
     1741 gtcggtggcg gtgacggttt gctcggtgaa cagccggtcg aagaggccct gatcggcgag
     1801 agccttcacc agctggcggt tgacccgccc gggcgatcct gtctccgcga tctcgatcaa
     1861 ggcgtggccg agtcgctcac attcctcgac gatcggccgg gtcactctcg gcgagaaatc
     1921 gagacgatcc gaggtgttcg gggtcatcga acgaataccc tacggcggtc acgtttcgat
     1981 cgcgagcgaa ggggagttag tcgatgggca acatgcacgc ggtccgaatt catgggtatg
     2041 ggggcacgga ggctctcgcg tacgaggagg ctccccgacc ccttccgcag gcttccgagg
     2101 tgctgatccg agtacatgcg acagcggtca atccgtttga tgtcgccttg cggtccggat
     2161 acttggtcgg ctatttcaac cacacgctgc ccctggtctt gggaactgac gtggctggcg
     2221 tcgtcgagga ggtgggcgcc ggggtagatg ggttctcgcc cggagacagc gtctatgcaa
     2281 ggtccggtgt cacccgcgac ggtgcgtacg ccgaatacgt cgtcgtccct gcggccgatg
     2341 tggcattcaa gccccaaacc ctcgatttcg ttcattcggc tgctctccct catgtcaccc
     2401 tcaccgcctg gcaggccctg ttcgaagccg ctgacgtggc cgaagggcag accgttctca
     2461 tccacgccgc ggccggcggc gttgggcacg ttgccgtgca actcgccaag ttgcgcggtg
     2521 ccaccgtgat tggaactgcg tctgagaaca tcgactttct tggcgaactg gaggtcgatc
     2581 aagccatcaa ctacgcgacc actccgttcg aggacgttgt cagcgacgtc gatgtcgttc
     2641 tcgacacagt gggcggcgac acccaggagc gttcatggtc cgttctcaag ccaggaggaa
     2701 tcctcgtctc ctccgtgcaa ccgccatcgg aggacgaggc gcaggcccac ggcgtccgtc
     2761 agcatttcat cttttccgtg ccgcccatag gtgaagtgct gactcaggtt gcagccatgg
     2821 cggatgcggg agacgtcacc ccgaaggttt cgacgattct ccctctgacg gatatccgaa
     2881 aggcccacga gttgatcgag acaggacata cgcggggcaa aatcggatta caggtcgtcc
     2941 cctgacagac cgctcgctgg tcgcaggcgt tcaacttcag cctcgcggtt agcctcctcg
     3001 ccctgtgagc ccatttcgcg cttctgcagg cctcaccgac agctgggagc atttcgggtt
     3061 cgaggtggcg cacggtgtgg ggacggtcac cttcgaccgt cctgacaagc tcaatgccct
     3121 cactttcgac gtctatgcag acctgaggga cctgctcggg gagatagagc atcgcgacga
     3181 catccgcgtt ctcgtcatca ccggcaccgg ccgaggcttc tgctccggtg gggac
//
LOCUS       DASTXO010000367         2775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1053767, whole genome shotgun sequence.
ACCESSION   DASTXO010000367 DASTXO010000000
VERSION     DASTXO010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053767"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFU96_07720"
     CDS             complement(<1..462)
                     /locus_tag="VFU96_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113193.1"
                     /translation="MTRQETFKRRIRQRMEKTGERYGAARRVLIEQSTTTEPRPWASE
                     PEMTDDTLRGATGRGWDEWCSILDAWPGHGDGHTAIATYLREEQAVEGWWAQTITVGY
                     ERITGLRLPHQQPDGTFTAGKSRTVAADAALIREMLLDDEARADLFPGLETE"
     gene            complement(665..2191)
                     /locus_tag="VFU96_07725"
     CDS             complement(665..2191)
                     /locus_tag="VFU96_07725"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEU5113194.1"
                     /translation="MARADSELRVTELLASLSLATDLGTGQPMGHGLATSLLAVAVAR
                     ELECRPEQIRHVQQVSLIRFLGCNADAGGTARMAGGNELNLMAAMSLAHMGSSGETLV
                     ALLRTAGAGQTLTTRARLVASALADTDADARSLSEHCEVGAMLARRLGLDQEVVIALQ
                     HSYERWNGGGHPGGLKGDEIPLETRIGIVARDVDLFARADRDVTGLLRDRQGKAYDPM
                     IVDVLIGLAQPHRDAEWNEVLGAEPPPIAYVDDIDQALAAVADYVDIKSAWTRGHSRQ
                     VADLANDAGRALGMSDQRRRDLRRAALVHDLGRVGVENGIWDKPGPLATGEREKVRLH
                     PYLTERILSRCPALARLGDVASSHHERLDGSGYHRQTTKVQTTEETMILAAADVMAAL
                     TADRPHRRQYDLEEAARLMEDEVAAGRLDHEAVTAVITAAGGQTTLVRAANPGSLTDR
                     EVEVLRLLARGETNRRIGEALFISPKTVGRHVENIYLKIEVSTRAGAALYAMEHRLLG
                     "
     gene            complement(2230..>2775)
                     /locus_tag="VFU96_07730"
     CDS             complement(2230..>2775)
                     /locus_tag="VFU96_07730"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU5113195.1"
                     /translation="GSVLAVIGIGWMALGPGEATPAVLPLLALFAATVCVGESVIVSK
                     MISANHPAITSAVSLTVGALTLLGMSALSGEHWALPRQSDVVWSLVYLVTLGSVGVFA
                     LVLLVVRRLTASATSYMFVLFPLVAMVAGPWLVDEPVTAPGVAGAVLVMIGVWLGALS
                     PGSSRSRPRPVDTTAVVLDSG"
BASE COUNT          477 a          957 c          861 g          480 t
ORIGIN      
        1 ctcggtctcc aggcccggga acaggtcggc tcgggcttcg tcgtcgagca gcatctccct
       61 gatcagcgcg gcgtcggccg ccaccgtcct cgattttccc gcggtgaacg tcccatcggg
      121 ctgctggtgg gggaggcgaa gcccggtgat ccgctcgtat cccacggtga tcgtctgggc
      181 ccaccagccc tcaacggctt gctcctcgcg tagatatgta gcgatggcgg tgtgaccgtc
      241 cccatgaccg ggccaggcgt cgaggatcga gcaccactcg tcccagccac gcccggtggc
      301 tcctcgcaaa gtgtcgtctg tcatctcggg ctcggacgcc cacggcctgg gctccgtggt
      361 cgtcgactgc tcgatgagga cacgacgggc ggcgccgtac ctttctccgg tcttctccat
      421 acgctgccgt atccggcgct tgaaggtctc ctgtcgggtc atttcaactg ctttctcgtg
      481 cctcaggccg catccaaccg gtgactcgcg ctcaccgacg catgcgtgga cacgcacagc
      541 tagaccgaca gttgccgagc actcgaggtc ccctttgcct tccgggtcgg ctgcgcgagc
      601 gccgagacga aggtcaggcg gggcaatccg cccacgacgc cagcttactt gcgtggcctc
      661 cccgtcatcc caggagacgg tgctccatgg cgtagagggc ggctccagct cgtgtcgaaa
      721 cctcaatctt gaggtagatg ttctcgacat gtctccccac cgtcttcggc gagatgaaca
      781 gcgcctcacc gatgcggcga ttggtctcgc cgcgtgccag caagcggagg acctccacct
      841 cccggtcggt cagacttccc ggattggccg ccctcaccaa cgtcgtttgc cctccggccg
      901 ccgttatgac cgccgtcact gcctcatgat cgagcctccc ggccgccacc tcatcttcca
      961 tcaaccgggc tgcctcctcg aggtcgtatt ggcgccggtg tggccgatct gcggttaggg
     1021 ctgccatgac gtctgctgcg gcgaggatca tcgtctcctc ggtggtctgc accttggtcg
     1081 tctgacggtg ataaccggac ccgtcgagcc gctcgtgatg actcgaggcg acgtccccca
     1141 gtctggcgag ggctggacat cgggaaagga tccgttcggt caggtaagga tggagcctga
     1201 ccttctcccg ttcaccagtc gcaagcggcc ctggcttatc ccagatccca ttctccacgc
     1261 cgacgcgacc caggtcatgg accaacgccg cccggcgcag gtcgcgacgt cgctggtcgc
     1321 tcattcccaa agcgcgtccg gcgtcattgg cgagatcggc cacttgccgt gagtgccctc
     1381 gagtccacgc cgatttgatg tccacatagt cggcgaccgc ggccaacgcc tgatcgatgt
     1441 catcgacgta cgcgatcggt ggtggctcgg caccgagcac ctcattccac tcggcgtctc
     1501 gatgcggctg cgcaagaccg atgaggacat cgacgatcat tgggtcgtag gcctttccct
     1561 gccgatcgcg cagcagtccg gtcacgtctc gatcggcccg ggcgaagagg tcaacgtcac
     1621 gagcaacgat tccgatgcgt gtctcgagcg gaatctcatc acccttgagg ccaccgggat
     1681 gcccaccacc gttccatcgc tcgtaggagt gctggagcgc gatgactacc tcctgatcta
     1741 gtcctagccg tcgggcaagc atcgccccga cctcgcagtg ctcggacagg cttcgggcgt
     1801 cggcatcggt gtcggccagc gccgatgcca cgagtcgggc gcgagttgtg agggtttgcc
     1861 ccgcgccggc tgtgcggaga agagccacca aggtctcacc ggagctaccc atatgggcca
     1921 gtgacatcgc cgccatcagg ttgagctcgt tgcctcccgc cattcgggcg gtgccgccgg
     1981 catcggcgtt gcacccgaga aaccgaatca gcgagacctg ctgcacatgg cggatctgtt
     2041 cgggcctgca ctccagctct cgggccacgg ctactgccag caggctggtg gcgagaccat
     2101 ggcccatcgg ctggcccgtc ccgagatcgg tggccagcga cagtgaggcc agcagctcgg
     2161 tgacacgaag ctccgagtcg gcgcgggcca tgagtccgac cctaccgacc gtcggatgag
     2221 tcgccctggt cagcccgaat cgaggaccac ggccgtcgtg tcgaccggtc tgggcctcga
     2281 gcggctcgag ccgggagata gtgcgccgag ccagacccca atcatcacca atacggctcc
     2341 agcgactccc ggcgcagtca caggctcatc gaccaaccac gggccggcca ccatcgccac
     2401 gagagggaag aggacgaaca tgtaggaggt ggcggacgcc gtcaaacgtc tgaccacgag
     2461 aaggaccaag gcgaacactc cgaccgaccc gagcgttacc aggtacacga gggaccacac
     2521 gacgtcggat tgacgtggca gggcccaatg ctccccggag agggccgaca tcccgaggag
     2581 agtcagcgcc ccaaccgtca gcgaaacggc gctggtgatc gccgggtgat ttgcagagat
     2641 catcttcgaa acgatgacgc tctcgccgac gcacaccgtc gcagcaaaga gagcaagcag
     2701 cgggagcacc gcgggcgtgg cctctccggg ccccagggcc atccagccga tgccgatcac
     2761 ggccagaacc gatcc
//
LOCUS       DASTXO010000368         3002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1127825, whole genome shotgun sequence.
ACCESSION   DASTXO010000368 DASTXO010000000
VERSION     DASTXO010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127825"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VFU96_07735"
     CDS             complement(<1..449)
                     /locus_tag="VFU96_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113196.1"
                     /translation="MNRTLRTLVVYGLSIIMLAILAQWWFDQVQGPEVIELSQFIDNV
                     EAGDYETVEMLARSNELQGRVTGSTLPEDTFEQVSSYPEGAEGDLVTVIRESGTEFSS
                     DPQPPGFWEVLVSFLPWLVLLGFMVLIFMQMQGGGIRVMQFGKSRAML"
     gene            complement(558..1079)
                     /gene="hpt"
                     /locus_tag="VFU96_07740"
     CDS             complement(558..1079)
                     /gene="hpt"
                     /locus_tag="VFU96_07740"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEU5113197.1"
                     /translation="MDIKPLITAEEIDSKLAELGRKITADYSGKDVLLVGVLRGAFMV
                     MADLARHIDLPVEFDFMAVSSYGASTQTSGVVRILKDLDEEIEGRHVLIVEDIIDSGL
                     TLNYLMKSLNVRKPASLEIAALLLKEGIQRVPLEVKYVGFPIGPEFVVGYGLDYAGKY
                     RNLPYVGVMGAEA"
     gene            complement(1061..2305)
                     /gene="tilS"
                     /locus_tag="VFU96_07745"
     CDS             complement(1061..2305)
                     /gene="tilS"
                     /locus_tag="VFU96_07745"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEU5113198.1"
                     /translation="MVETRRLKELRRRSTGLLRLPDGDLVIALSGGADSATLAYLVSR
                     LSREARGVHVNHGLPSSDRLEAAAGAVAAAVELALEVRHIDIPSGPSIEGQARKARYD
                     ALSGALRPGETLLTAHTLDDQAETVLMSLLRGAGPTGLAGIPPRTGSLARPMLAVSRS
                     DTRELAGLAGLPFYDDPSNLDRGLRRNEVRLEVIPDLAARFNPKLVESLARSAEVVRS
                     DETFMQREAASVPIVEAGASLSLPLGALFAVPRPVADRVLRRCLARMRPPHGGIAEEM
                     AAIWVVARRERQSVVLGGGLAVAHEGPMLVFQPVTTPPPKARVDLEIGTNAVGPFRVV
                     VDRVDRACRVAPIGLWSAVFPPDVTLAARLDDNGRLVIAANEETAWLPGERRLPVAWY
                     EPGTNGYLSVFAREESGWTSSP"
     gene            complement(2284..>3002)
                     /locus_tag="VFU96_07750"
     CDS             complement(2284..>3002)
                     /locus_tag="VFU96_07750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEU5113199.1"
                     /translation="AELTAVLGVIARRVLGQYELVLPTGEDADVVAFVGPNIIQLERA
                     HQFRPEEFRFWVTLHELTHRAQFQGVPWLREYFMSLVTELVEHSRPEPGRMNRIIDEV
                     SRRRGEGTPIIDERGLLGLFASSEQTVVIDKVQALMSLLEGHGHVVMDRLGSQHLRTQ
                     ERMSNVLKARRKDKRTAAFFRLTGMEMKMNQYRMGERFIEAVEREAGWEAVGMAFRGA
                     SSLPTLEEIESPERWLRRVA"
BASE COUNT          565 a          999 c          937 g          501 t
ORIGIN      
        1 agcatggcgc gggacttgcc gaactgcatg acccggatgc cgccgccctg catctgcatg
       61 aagatcaaca ccatgaaacc gagcaggacg agccagggca ggaagctgac caggacttcc
      121 cagaacccgg gtggctgggg gtccgagctg aattcggtgc ccgactcccg gatcaccgtg
      181 accagatcgc cctccgcccc ctcgggatag ctggaaacct gttcgaacgt gtcctcaggc
      241 agcgtcgagc cggtgacacg gccctggagc tcattggagc gggccagcat ctcgaccgtc
      301 tcgtagtcgc cggcctcgac gttgtcgatg aactgagaga gctcgatgac ctctgggcct
      361 tggacctggt cgaaccacca ctgggcgagg atcgccaaca tgatgatgga cagtccgtaa
      421 acgacgagcg tgcgcagggt tcggttcact gaggcctcaa gacaaactcc ggggaagact
      481 ccgagacgtc aatgctacgg gaatcccttg gatagtcgaa ccccttctcg gcagcggggg
      541 aggacgacga gtcgggctca ggcttcggca cccatgacac cgacataagg caggttccgg
      601 tacttgcccg catagtcgag gccgtaaccg actacgaact cggggccgat cgggaatccc
      661 acgtacttga cctcgagcgg cactcgctgg atgccttctt tgaggaggag tgctgcgatc
      721 tcgagggagg ccggtttgcg cacgttgagg ctcttcatca gatagttcaa ggtcagaccc
      781 gagtcgatga tgtcctccac gatcaagacg tggcgtccct cgatctcctc gtccaggtcc
      841 ttgaggatgc ggacaacacc cgaggtctgg gtcgacgccc catacgaaga caccgccatg
      901 aagtcgaact ccaccggcag gtcgatgtga cgggccaggt ccgccatgac catgaacgcc
      961 ccccggagca cgccgacgag caagacatcc ttccccgagt aatccgccgt gatcttccgg
     1021 ccgagctcgg cgagcttgct gtcgatctcc tcggcggtga tcaggggctt gatgtccatc
     1081 cgctttcctc tctggcgaac acggaaaggt agccgttggt ccctggctca taccaggcca
     1141 cagggagccg ccgctcgccc ggcaaccagg ccgtctcctc gttggccgcg atcacgagcc
     1201 gcccgttgtc gtccaggcgt gccgccagtg tcacgtcggg agggaagacc gccgaccaca
     1261 ggccgatggg cgccacacgg catgcacgat caacccgatc gacaacgacg cgaaacgggc
     1321 cgaccgcgtt ggtgccgatt tccaggtcga cccgggcttt tggaggcgga gtggtgacag
     1381 gctgaaagac gagcatcggg ccctcgtggg ccaccgccaa tcccccgccc aggacgacgg
     1441 actgtcgttc acgccgggca accacccaga tggccgccat ctcctcggcg atcccaccgt
     1501 gtggcggtcg catccgggcc aggcatcgtc tcaagacccg gtccgcaacc ggtcggggga
     1561 cggcgaacag cgctccgagt gggagggaca acgacgcccc ggcctcgacg atgggaacgg
     1621 aggctgcctc acgctgcatg aaggtctcat cagaacgtac gacttccgcg gacctggcca
     1681 gcgactcgac cagcttcggg ttgaaccgtg cggccagatc cgggatgacc tccaacctca
     1741 cctcgttccg gcgcaggcca cggtcgaggt tggacgggtc gtcgtagaaa gggagtccgg
     1801 ccagaccggc caactccctc gtgtcggagc ggctaaccgc gagcatgggc cgggccaagg
     1861 agccggtgcg gggcgggatt ccggccagcc cggtagggcc ggctcctcgg agcaggctca
     1921 tcaggacggt ctcggcctgg tcatcgagcg tgtgagccgt gagcaacgtc tctcccggtc
     1981 gcaaggctcc tgagagggcg tcatacctcg ccttgcgcgc ctgaccctcg atcgaagggc
     2041 ccgacgggat gtcgatatgt cggacctcca atgcaagctc gaccgccgcc gcaacggctc
     2101 cggccgccgc ctccaatcga tcggacgagg gcaagccatg gtttacgtgt actcccctgg
     2161 cctctcggct cagccggctg actagatatg cgagcgtggc cgagtcggcg ccaccggaga
     2221 gggctatgac cagatcgccg tcgggtagcc ggaggagacc cgtagacctc ctcctcagct
     2281 ctttcaggcg acgcgtctca accatcgctc gggactctcg atctcctcga gggtgggcag
     2341 gctgctcgcg cccctgaagg ccatgccgac ggcctcccag ccggcctcgc gctcgacggc
     2401 ttcgatgaat ctctccccca tccggtactg gttcatcttc atctccatgc ccgtgagcct
     2461 gaagaacgcc gcggtccgct tgtctttccg tcgggctttc aagacgttgc tcatccgctc
     2521 ctgcgtgcgc agatgctgcg agccgagacg gtccatcacc acgtggccgt gaccttcgag
     2581 cagcgacatc agcgcctgga ccttgtcgat cacaacggtt tgctccgagg aggcaaacaa
     2641 cccgagcagc ccacgctcgt cgatgatcgg cgttccctct ccacgacgac ggctgacctc
     2701 gtcgatgatc cgattcattc gcccgggctc gggacgggag tgctcgacca gctcggttac
     2761 cagactcatg aagtactcac gaagccaggg gacaccctgg aactgagcgc ggtgggtcaa
     2821 ctcatgaagg gtgacccaga acctgaactc ttccgggcgg aactgatggg cgcgctcgag
     2881 ctggatgatg ttcggaccca cgaaggcgac gacatcggca tcctcgccgg tcggaagaac
     2941 caactcgtac tgacccaaca cgcggcgagc aatgactccg aggaccgccg tcagctcggc
     3001 ct
//
LOCUS       DASTXO010000369         2118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1532795, whole genome shotgun sequence.
ACCESSION   DASTXO010000369 DASTXO010000000
VERSION     DASTXO010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1532795"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..973
                     /gene="cofD"
                     /locus_tag="VFU96_07755"
     CDS             62..973
                     /gene="cofD"
                     /locus_tag="VFU96_07755"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEU5113200.1"
                     /translation="MTRVVELSGGVGGARLARGMDALPDVDLTVVVNVGDDLPLHALH
                     ICPDLDTMVYTLAGVEGPYGWGRAGDSFRANTELARFGVENGFQLGDLDLALKIYRSD
                     RLSQGDTLTEVTALVCAGFGIRSRILPASDDPVRTMVTTTDGSRLTFREYFVDRGHRD
                     HVGKLEFDGAQDAKPAPGVLEAIDSADLLVIGPSNPPLSIWPILAIEAIEQAVRRHPN
                     VIAVSPLIGGRTMKGPADVVMADLGLGSGSGAVLASYDGLVDTLVVDGTDSADEGEFD
                     GVHVAALETQIADPADAATLARAILGL"
     gene            970..1668
                     /gene="cofE"
                     /locus_tag="VFU96_07760"
     CDS             970..1668
                     /gene="cofE"
                     /locus_tag="VFU96_07760"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEU5113201.1"
                     /translation="MTLQIVPLTGIGPVHPGDELTDILIDALAPLSPGSGDVLVVTHK
                     IVSKAEGRVVTIEGDEEEFKRTLVESEAVAVVRRRGSLIIAETEHGFICANAGVDRSN
                     AEPGTMVLLPRDPDRSAHRIRVRLRQSFDVDLPVIISDTFGRPWRRGLTDIAIGVSGL
                     EAVIDLKGTNDWAGRELEVTEIAVADELAAAADLVMGKADGIPAALIRGYQGPRGEGR
                     GSDLVRPPDEDMFR"
     gene            complement(1693..>2118)
                     /locus_tag="VFU96_07765"
     CDS             complement(1693..>2118)
                     /locus_tag="VFU96_07765"
                     /inference="COORDINATES: protein motif:HMM:NF015665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HEU5113202.1"
                     /translation="EADEEQRRRVADKVRQAAGGGLRGRRVGMWGVAFKAGTDDVRES
                     PAVRIASLLQADGAEVVAYDPEATTDLLTMAPDPIAAVTEADVLLIATEWPEFLTVDM
                     AEVSKVMRGHRVVDARNILDPKKVRSVGLDYWGLGRPGA"
BASE COUNT          370 a          693 c          677 g          378 t
ORIGIN      
        1 gcacttgtca tgcgggagga cattgtgaac cggagcgcgt cactgagcaa tatcgagagc
       61 cgtgaccagg gtcgttgagc tttccggagg cgtcggaggt gcgcgtctgg cgcgtggcat
      121 ggatgctctc cccgacgtcg atctcacggt tgtggtcaac gtcggagacg atctgcccct
      181 tcacgcactg catatctgcc ccgacctcga caccatggtc tacaccctgg ccggcgtcga
      241 aggcccctac ggctggggac gtgccggcga tagcttccga gccaacaccg agctggcccg
      301 cttcggcgtc gagaacgggt ttcaactcgg agatctcgat ctggccctca agatctatcg
      361 cagcgaccgt ctttcgcagg gtgacacttt gaccgaggtg accgctttgg tgtgcgccgg
      421 tttcgggatc cgatccagga ttctgcccgc ctccgacgat ccggttcgga ccatggtcac
      481 gaccacagac gggagcaggc tcaccttccg ggaatacttc gttgaccgag gtcatcggga
      541 tcatgtgggc aagctcgaat tcgacggagc acaagacgcg aagccggccc ctggcgtact
      601 cgaggcaatc gacagtgccg acttgttggt gatcgggcca tccaatcccc cgctgtccat
      661 ctggccgata ctcgccatcg aggcgatcga acaagcggtc cggcgccacc ccaatgtgat
      721 cgcggtcagc cccttgatcg gcggccgcac catgaaaggc ccggccgatg tggtgatggc
      781 tgacctgggt ctgggatccg ggtcgggcgc agtgctcgct tcctacgacg gtctcgtcga
      841 cacgctcgtg gtcgatggca ctgacagtgc cgacgaaggt gaattcgatg gtgtccacgt
      901 cgcggcgctc gaaacccaaa tcgccgaccc ggcggatgcg gcaacgctgg cccgggcgat
      961 actgggcttg tgaccctcca gatcgtcccg ctgaccggga tcggccctgt tcatccgggc
     1021 gacgagctca ccgacatcct catcgacgcg ctcgctcctt tgagccccgg gagtggagac
     1081 gtgctggtcg taacccacaa gatcgtctcc aaggcagaag ggagggttgt caccatcgaa
     1141 ggggacgaag aggagttcaa gcgcacactc gtggaatctg aagcggtcgc cgtggtgaga
     1201 cgacggggca gcctcatcat cgccgagacc gagcacgggt tcatttgtgc caatgccggt
     1261 gtcgaccgtt cgaatgccga gccgggaacc atggtccttc tccccaggga cccggatcgg
     1321 tcagcccatc gcatcagagt tcgcctccga cagagtttcg acgtcgacct cccggtcatc
     1381 atctccgaca ccttcgggcg accatggcgc cgcgggctga ccgacatcgc gatcggggtg
     1441 tcgggcctcg aggcagtgat cgacctgaag ggcacgaatg actgggccgg gcgcgagctc
     1501 gaggtgaccg aaatcgcagt agccgacgag ctcgccgccg ctgccgatct cgtcatgggt
     1561 aaggcggatg ggatccccgc cgcattgatc cgtggttacc agggcccgcg tggcgagggt
     1621 cggggctcgg atctggtccg gccacccgat gaggacatgt tccggtgatg acggacctgg
     1681 gcgtgggacc ctctatgcgc ccggacggcc cagaccccaa tagtcgaggc cgaccgacct
     1741 gacctttttc gggtccagga tgttgcgcgc atcgaccaca cggtgtcctc gcatcacctt
     1801 ggagacctca gccatgtcca ccgtgaggaa ctcgggccac tcggtggcga tgagcaacac
     1861 gtcggcttcg gtcaccgccg cgatcggatc tggtgccatc gtcagcaaat cggttgtcgc
     1921 ctcggggtcg tatgcgacca cctcggcgcc atcggcctgg agcaaggaag cgatccgaac
     1981 ggccggcgac tcgcggacgt cgtccgtgcc cgccttgaag gcgacgcccc acatgcccac
     2041 ccggcgccct cgcagaccac cacccgcagc ctgccgaacc ttgtcggcga ctcgccggcg
     2101 ctgctcctcg tcggcttc
//
LOCUS       DASTXO010000370         2148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_280835, whole genome shotgun sequence.
ACCESSION   DASTXO010000370 DASTXO010000000
VERSION     DASTXO010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280835"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2148
                     /gene="secA"
                     /locus_tag="VFU96_07770"
     CDS             <1..>2148
                     /gene="secA"
                     /locus_tag="VFU96_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU5113203.1"
                     /translation="FRGRIAAGQDLDDLQVEAFAAVREAAKRVLGQRHFDVQIIGAAA
                     LHRGMVAEMKTGEGKTLVSTMPAYLNALNGNGVHLVTVNDYLASRDADWMGAVHRFLG
                     LKVGLIQNGMSSAERHPAYDSDITYGTNNEFGFDYLRDNMAMRADDRVQRGHTYAIVD
                     EVDSILIDEARTPLIISGRVSDSARWYRDFAKIVNRLQKDIHYEVDERKRQVLTTEEG
                     VTRVEQVLGIENLFDHAAVDFVHHLEAALRAKELYLRDVDYLIDHGEVKIVDEFTGRV
                     LEGRRYSEGLHQAIEAKEGVAIKDENQTLATITLQNYFRLYDKLAGMTGTAETEAAEL
                     AQIYSLEVIGIPTNEPVAREDEGDLVYKSEDGKYGALVADVAARAENGQPVLLGTVSI
                     EKSEKLSKELEHRGVKHEVLNAKHHAREADIIAQAGRPGAVTVATNMAGRGVDIKLGG
                     NPDGMARTELKRRGITHDDPSYEELEQKLIQEFDEQITPDKQRVIDAGGLYVVGTERH
                     ESRRIDNQLRGRSGRQGDPGESRFYLSLEDDLMRRFQGEWVTGIMDRLRLPEDQPIEA
                     NMVSKAIERAQRQVESQNFEIRKNVLKYDEVMNRQREVVYEWRRSILEGTAGDDLIGE
                     WIDSVVTETVESEISSALPRSDWDWEALSRELNQVYPTSIDESKVAGTADDVVEFAVE
                     EALDAYAKREEELGAELLRQVERSVMLSVIDNK"
BASE COUNT          456 a          621 c          723 g          348 t
ORIGIN      
        1 gttccgcggt cggatcgctg ccggccagga tctcgacgac ctgcaggtcg aagcctttgc
       61 cgccgttcga gaggcggcga agcgcgtgct tggccagcgg catttcgatg tgcagattat
      121 cggggcggcc gcactccacc gggggatggt ggcggagatg aagaccggcg agggcaagac
      181 tctggtgtcg acgatgcccg cctacctcaa cgctctcaac ggcaacgggg ttcatctggt
      241 caccgtcaac gactacctgg catcccggga cgccgactgg atgggcgccg tccatcgctt
      301 cctcggattg aaggtgggtc tcattcagaa cgggatgtcc tcggctgagc gtcatcccgc
      361 ctacgactct gacatcacct acgggaccaa caacgagttc ggcttcgact acctcagaga
      421 caacatggcg atgcgcgccg acgaccgggt gcagcgaggg cacacctatg ccatcgtcga
      481 cgaggtcgat tcgatcctga tcgacgaggc gcgaacgcca ttgatcatct ccgggcgagt
      541 atctgacagc gccaggtggt accgcgattt cgccaagatc gtcaaccggc tccagaagga
      601 catccactac gaggtcgatg agcgaaagcg acaggtgttg acgaccgagg aaggcgtgac
      661 gcgggtcgag caggtgcttg ggatcgagaa cctcttcgac cacgccgcgg tcgacttcgt
      721 ccaccacctc gaggcggcat tgagggcaaa ggagctctat ctgcgcgatg tcgactatct
      781 gatcgatcac ggcgaggtga agatcgtcga cgagttcacc ggccgggtcc tcgaaggtcg
      841 gcgttattcc gaagggctcc atcaggcgat cgaggccaag gaaggtgtgg cgatcaagga
      901 cgagaaccag actctggcca cgatcacact ccagaactat ttccggctct acgacaagct
      961 ggccgggatg accggcaccg ccgagacgga ggcggccgag ctggcccaga tctactcact
     1021 cgaggtcatc ggaatcccga ccaatgagcc ggtggcgcgg gaggacgagg gtgacctggt
     1081 ttacaagagt gaggatggga agtacggggc cctggtggcc gatgtggccg cccgggccga
     1141 gaatgggcag ccggtgttgc tcggcacagt gtccatcgag aagtccgaga agctctccaa
     1201 ggagctggaa catcgcgggg tcaagcacga ggtgctcaac gccaaacacc acgcccggga
     1261 ggccgacatc atcgcccagg cggggaggcc cggggcggtc accgttgcca ccaacatggc
     1321 cggccgtggc gtcgacatca aactcggggg caatcccgac ggaatggccc ggaccgagct
     1381 gaagcgacga gggatcactc acgacgatcc ctcctatgaa gagctggagc agaagctgat
     1441 ccaggagttc gatgagcaga tcacacccga caagcaacgg gtcatcgatg ccggtggtct
     1501 atatgtggtc gggacggagc gtcacgagtc acgacgcatc gacaaccagc tcagaggccg
     1561 ttccgggcgg caaggtgacc cgggggagtc ccgtttctac ctatcgctcg aagacgacct
     1621 gatgcggcgg ttccaggggg agtgggtcac tgggatcatg gaccgtttgc gtctcccgga
     1681 agaccagccg atcgaggcga acatggtctc gaaggcgatc gaacgggcgc agcgccaggt
     1741 cgagtcccag aacttcgaga tccgcaagaa cgtcctgaag tacgacgagg tgatgaaccg
     1801 ccagcgtgag gtggtgtacg aatggcggcg ctcgatcctc gagggaaccg ccggagacga
     1861 cctgatcggc gagtggatcg actcggtagt gacggagacg gtcgagtcgg agatctcatc
     1921 ggccctccca cgttcggact gggactggga ggcgctgagt cgcgagctca accaggtcta
     1981 tccgacctct atcgatgaat cgaaggtcgc cggcaccgcc gatgacgtcg tggaattcgc
     2041 agtcgaggag gcgctcgacg cctacgccaa acgtgaagag gagttggggg cggagctgct
     2101 ccgccaggtg gaacgatcgg tgatgctgtc ggtgatcgac aacaaatg
//
LOCUS       DASTXO010000371         2774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_22648, whole genome shotgun sequence.
ACCESSION   DASTXO010000371 DASTXO010000000
VERSION     DASTXO010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22648"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VFU96_07775"
     CDS             <1..249
                     /locus_tag="VFU96_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEU5113204.1"
                     /translation="HAVATNRDWAEDNDFHFPILSDYWPHGEVSRAYDTFDETFGYAK
                     RTTYVLDSDGVIRDVIASDVLKEARPFASYLPALESIR"
     gene            417..2132
                     /locus_tag="VFU96_07780"
     CDS             417..2132
                     /locus_tag="VFU96_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020108608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEU5113205.1"
                     /translation="MTARISAPLQAWWSSVRPGRGELRREAVAGLPGAIGGVPDGMAA
                     AVLAGVSPVFGLYASFAGPVAGGLTSSTGLMVITTTTASALAAGSSLAAVSSEDRAAS
                     LFMLVILAGALMVLAGILRLGRFTRFVSHSVMIGFLTGVAANIVFGQLPDLTGVDAEG
                     ANSVAKGIDVILHPGSIDLPSLLAGLGTILLIVVLGRTKVAPYASIVALAIPTILVLG
                     IDSVATVDDIGEIPSGIPLPALPDISVINGDLILVAFAIAAIVLVQGSGVAEAAPNRD
                     GSLSNPNRDFIAQGAGNIASGFFKGMPVGGSVGQTALNVSAGARGRWASIFSGIWMFV
                     ILALFSGIVGVVAMPTLAAILIVAAIGSLRVGQIQSILRTGPTSRIAMIATFVATLIL
                     SIPQAVGVGVIISLLLQLNQEALDLRVVELVPQPGGSFEERPAPDRLSSHSVTLLDVY
                     GSLFYAGARTLQANLPEVGTAIQPAVVLRLRGRTMLGATAFKVFSDYSEALRRAQGKL
                     YLSGVDAALMEQFSRAGHVEAGPVEVVEATPNLGESSRTAYHQAETWVIGHNAAEGPE
                     SRSDR"
     gene            complement(2129..>2774)
                     /locus_tag="VFU96_07785"
     CDS             complement(2129..>2774)
                     /locus_tag="VFU96_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113206.1"
                     /translation="GEEPEPRYTVDEGTETEYLGREATLYRMTGDGVVRAELVFDVEA
                     GALLRVVTFNADGSVFCERRFVTFDPGAPPPTDAEAAPSVESAEGGVESSLPETLSNF
                     DRLDVYQDEEGFIFAYYSDGFFSFAVFQTPAIVASDGGSLVTIGERTYIRSSGAGHVT
                     YAWETSSGGMALVGDLPPDMHQDVLSGLPAPEDPGWFRRLWRNLFGQTGSARPL"
BASE COUNT          463 a          908 c          896 g          507 t
ORIGIN      
        1 cacgccgtgg ccaccaaccg ggattgggcg gaagacaacg acttccactt cccgatcctc
       61 tccgactatt ggccacacgg cgaggtctcc cgggcgtacg acacgttcga cgagacattc
      121 ggctacgcca agcggactac atacgtcctc gattccgacg gcgtgatcag agatgtgatc
      181 gcgtcagacg ttctgaaaga ggcacggcct ttcgccagct atctgcctgc tctggaatcg
      241 atcaggtaac tcagatcacc cactctgggt gaggccggca cgcctgagag ctgtgcaagt
      301 accggtgagg ccttggcgac ccaggcacga tcaacgtcgg tttgcgagga tacgagcatg
      361 accgaagcca cgccgtcacg atgatcgcca tcatccctga aggtgcctga cgggcgatga
      421 cggcacgaat ctcagcgccg ctccaggcat ggtggtcatc ggttcgaccc ggccggggtg
      481 agctacgacg ggaggctgtc gccggcctgc ctggggccat cggcggtgtt cccgacggga
      541 tggcggctgc tgtcctcgcc ggcgtgagcc cggtattcgg gctctacgcg agctttgcgg
      601 gccctgtcgc cgggggtctc acctcgagca ccgggttgat ggtcatcacc accacgacgg
      661 cctcggccct cgccgccgga tcgtccctgg cggcggtgtc gtccgaggac cgggccgcct
      721 cattgttcat gttggtgatc ctggccggcg ccctcatggt gctagccggc atcctcagat
      781 tgggacggtt cacccggttc gtctcccatt cggtgatgat cggattcttg accggagtcg
      841 ccgccaacat cgtgttcggt cagctccccg acttgaccgg tgtcgatgcg gagggcgcca
      901 attcggtggc caaaggcatc gatgtgatcc tgcatccggg cagcatcgac cttccgtcgc
      961 tccttgcggg tctgggcacc attctgctca tcgttgtcct cgggagaacc aaagtcgccc
     1021 cgtacgcctc gatcgtcgcc ctcgccatac cgacgatcct ggttctgggc atcgacagcg
     1081 tggccacggt cgacgacata ggtgagatcc ccagcggaat tccgcttcct gccctccccg
     1141 atatcagcgt gatcaatggg gacctgatcc tcgtcgcctt cgccatcgca gccatcgtgc
     1201 tcgtgcaagg gtcgggggtg gccgaggcgg cgccaaaccg tgatggctca ttgtccaatc
     1261 cgaaccggga tttcattgcc cagggagcag gcaacatcgc atcggggttc ttcaaaggga
     1321 tgccggtcgg gggctctgtc ggtcagaccg cgctgaatgt cagcgcggga gcccggggcc
     1381 gttgggcgag catcttctca ggcatctgga tgttcgtgat cctggctttg ttctcgggca
     1441 tcgtcggagt cgtcgcgatg ccgacgctcg cggcgatcct catcgtcgcc gccatcggct
     1501 ccctgcgcgt gggtcagatc cagtcgatcc tccgcacggg cccgacctcg aggatcgcga
     1561 tgatcgccac gtttgtcgcc accctcatcc tgtccatccc gcaggcggtg ggtgtgggcg
     1621 tgatcatctc cctgttgctc cagctgaacc aggaagcact cgatctgcgg gtagtcgagc
     1681 tcgtccccca accgggcgga tccttcgagg agaggcctgc tccggatcgg ctttcgagtc
     1741 attcggtcac gctgctcgac gtctacggga gcctcttcta cgccggggcc aggacgctgc
     1801 aggccaacct gcccgaagtg ggcacggcca tacagccggc cgtggtgctc cgcctccggg
     1861 ggcgaacgat gctcggcgca accgcgttca aggtgttcag cgactactcg gaggcgttgc
     1921 gacgcgctca aggcaagctg tacctgagcg gggtcgacgc ggccctgatg gaacagttca
     1981 gccgtgccgg acatgtcgag gccggtcccg tggaggtggt cgaagcgacg ccgaacctgg
     2041 gcgagtcgag tcgcaccgcc taccaccaag ccgagacctg ggtgatcggg cacaacgcgg
     2101 ccgaaggccc ggagtctcga tccgaccgct agagaggccg ggccgaacct gtctggccga
     2161 acaggttccg ccagagccgg cggaaccaac cgggatcctc gggggcgggg agaccggaga
     2221 gcacgtcttg gtgcatatcg ggcggcaggt caccgaccag cgccattcct cctgagctcg
     2281 tctcccaggc gtaggtgaca tggccggcgc cggatgatcg aatgtaggtc cgctctccga
     2341 tcgtgaccag tgagccgcca tcggaagcca cgatcgccgg cgtctggaac acggcaaacg
     2401 agaagaaccc atccgagtag taggcgaaga tgaagccttc ctcgtcctga tagacgtcga
     2461 gccggtcgaa gttgctcaag gtctccggaa gactcgactc gacgccacct tcggcagact
     2521 cgacgctcgg ggcggcctcg gcgtcggtgg gcggtggcgc gccgggatca aaggtgacga
     2581 agcgacgctc gcagaagacg cttccgtctg cgttgaaggt caccacccga agcagggccc
     2641 cggcttcgac gtcgaagacg agctcggctc ggaccacccc gtcgccggtc atccgataca
     2701 aggtggcttc gcggcccaag tattcggtct cggtcccctc gtccaccgtg tagcgcggct
     2761 ccggctcctc gccc
//
LOCUS       DASTXO010000372         3939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1274997, whole genome shotgun sequence.
ACCESSION   DASTXO010000372 DASTXO010000000
VERSION     DASTXO010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274997"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VFU96_07790"
     CDS             <1..429
                     /locus_tag="VFU96_07790"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU5113207.1"
                     /translation="LFRVPVLAQGMRMVGIIEVDRQARGAVHSEVNRQSRELIEKGRS
                     LIIYAEGTRPRNGVMKPFKKGAFTMAISSGLPVLPLSIHGSYEAWPPGTPLVRGGLIK
                     VVLDKPVETEGMTTSDTGDLRNQVREVIAGRVEAMGGAVG"
     gene            511..636
                     /locus_tag="VFU96_07795"
     CDS             511..636
                     /locus_tag="VFU96_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113208.1"
                     /translation="MANKDKGGKSTKKVAAKDLKEKRQAKKDKKAVAASASRVTR"
     gene            complement(652..1800)
                     /locus_tag="VFU96_07800"
     CDS             complement(652..1800)
                     /locus_tag="VFU96_07800"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEU5113209.1"
                     /translation="MRIGILTGGGDVPGLNACIKAVVNRAAEEGHQVVGIRRGWGGLA
                     HFNPEDAESNGDLLISLDPGVVRTIDRTGGTFLHTSRTNPGKMRPEEVPAFLGDKSFE
                     EGSVDITPHVLGVLEDQKIDVLVPIGGDDTLSYGLRLHEEGVPVIAIPKTMDNDVHGT
                     DYCIGFSTAVTRGVQFIHSLRTAAGSHERIAVVELFGRYSGETSLVTAYLSGVDRAII
                     SEVPFDIEKLAGMLLADRASNPSNYAMMTISEGARLQEGDMMLAGEEDAYGHRKLGGI
                     GALTGELLKERTGEPVLYQQVAYLMRSGAPDSLDLMVATNYAVMAADLAVENSHGRMV
                     ALRGGTYTHVPIGVTKEGVKRVDVDELYDPETYLPKVRHVLGKPMFLY"
     gene            complement(1834..2391)
                     /locus_tag="VFU96_07805"
     CDS             complement(1834..2391)
                     /locus_tag="VFU96_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEU5113210.1"
                     /translation="MGKVIYGMNVSIDGYIEDSSGSIAFSGPPADDVHRHWNERTEAA
                     AASLYGRRLFEVMDEHWTAAAMQDDLPDVEAEFARAYVGTPRFVFSDTLESVPSGVEL
                     VRSRDAAAVVSRLKDEIEGPLALGGAGLAASLLDLIDEFQLVVLPVVVGSGKPFFPAG
                     SELPLRLVDQRVFDSGAVYLAYVRR"
     gene            complement(2409..2717)
                     /locus_tag="VFU96_07810"
     CDS             complement(2409..2717)
                     /locus_tag="VFU96_07810"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEU5113211.1"
                     /translation="MSDLAVVVAVAAITYGTRVTFLLRPRSVPEGALGRFLDVFPLAL
                     FVAIAITGLVAPEGEPAVTPALAAALGGIIGAVVFRRNLWGVLVVGMVFFYITRALTA
                     "
     gene            complement(2710..3378)
                     /locus_tag="VFU96_07815"
     CDS             complement(2710..3378)
                     /locus_tag="VFU96_07815"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEU5113212.1"
                     /translation="MLDNPSFRQGIRDSVLIILAVAGFGVAYGVLAVRAGFPPWLAVF
                     SSVIIVSGAAQFAMIGLLSSGPVAVLLAATGLGLRHIPMSASLSNMIGPRPLGTRLRL
                     AFILVDETFGLTVRAADAGVEDLVAYKTAADLMLYSGWVVGTTIGAWFGGSIDTEAVG
                     IGVLFPLLFLGLAAPMIKSRKDLLVAGAAVVASVVATAVLPAAWQITVAAVVASMAGV
                     FVGE"
     gene            complement(3431..>3939)
                     /locus_tag="VFU96_07820"
     CDS             complement(3431..>3939)
                     /locus_tag="VFU96_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5113213.1"
                     /translation="GDPTDPATAIGPLINQRAVVKVHTQVEEAVAAGANLVTGGRHDN
                     LVYHPTVVTDVTRDMQLFREQTFGPVAPIVVVDDAEEALAVANDSDYGLSSGILTSDF
                     NRALDMAMRLETGMVHIGDQTVNDEPQVPFGGVKGSGYGRFGGQSALDEFTELRWINV
                     QRVPRTFP"
BASE COUNT          784 a         1262 c         1257 g          636 t
ORIGIN      
        1 ctgtttcggg tcccggtgct ggcacaggga atgagaatgg tcgggatcat cgaggtggac
       61 cggcaggcga ggggcgcggt gcattcagaa gtgaatcgtc agtcgcgaga gctgatcgag
      121 aagggtcgaa gcctcatcat ctacgccgag gggacccggc cccgcaatgg cgtgatgaaa
      181 ccattcaaga agggcgcctt caccatggcg atcagctcgg gcctgccagt gctccccctg
      241 tcgatccatg gttcgtacga agcctggcct ccgggcacgc cgttggtcag gggcgggctg
      301 atcaaagtcg ttctcgacaa gccggtggag accgaaggaa tgacgacctc ggacacaggc
      361 gacctgcgaa atcaggtgcg tgaagtgatc gcgggtcggg tagaggcgat gggcggcgcc
      421 gtcggctagc agggggaaca tccgctggct ctcgatacgt gacacactgg aggagaatcg
      481 acttcggttc gcccaacaga aaggccagaa gtggctaaca aagacaaggg cggcaagagc
      541 accaagaagg tggcagccaa ggacctgaag gaaaagcgcc aggccaagaa ggacaagaag
      601 gctgttgccg cctcggccag ccgcgtgacg cgctagacga atgtcactct gtcagtagag
      661 gaacatcggc ttgcccagca cgtgccgcac cttgggcagg taagtctccg ggtcatagag
      721 ctcgtcgacg tcgacccgct tcacgccttc cttggtcacc ccgataggga catgggtgta
      781 tgtgccgccg cgaagggcca ccatccgacc gtggctgttc tcaaccgcca ggtctgcggc
      841 catgaccgca tagttggtgg ccaccatcag gtcgagcgag tcgggcgctc ccgaccgcat
      901 caggtatgcc acctgctgat agagcaccgg ctcaccggtg cgctccttga gcagctcgcc
      961 ggtgagggcg ccgatcccac ccagcttgcg atggccgtag gcgtcctcct cgcccgccag
     1021 catcatgtct ccctcttgga gccgggcgcc ttcggagatc gtcatcatgg cgtagttcga
     1081 tgggttcgat gcccgatccg ccaaaagcat gcccgcgagc ttctcgatgt cgaaaggaac
     1141 ctcggagatg atggcccgat ccactccgga caggtacgcg gtcaccagtg aggtctcgcc
     1201 gctgtaccgc ccgaacagct cgactaccgc aattcgctcg tgggagccgg cggcggtgcg
     1261 cagactatgg atgaattgca ctcctcgggt caccgccgtc gagaagccaa tgcagtagtc
     1321 ggtgccgtgg acgtcgttgt ccatcgtctt ggggatggcg atgaccggca ctccctcctc
     1381 gtggaggcgc agcccgtagg acagggtgtc atcgccaccg atcgggacca ggacgtcgat
     1441 cttctggtct tcgaggaccc ccagcacatg tggggtgatg tcgacgctcc cctcctcgaa
     1501 cgacttgtcg cccaggaagg caggaacctc ctccggcctc atcttgcccg ggttggtccg
     1561 ggaggtgtga aggaaggttc caccggtgcg atcgatggtt cggaccactc cgggatcgag
     1621 tgagatcagc aagtcaccgt tcgattcggc gtcttcgggg ttgaaatggg ccagccctcc
     1681 ccatccacgg cggatgccga ctacttgatg cccctcttca gccgcccggt tcactaccgc
     1741 tttgatgcaa gcgttgagcc cgggcacgtc gccaccaccg gtcaagattc cgattcgcat
     1801 ggagcgggag cgtaacgccg tggggagggc gagctaccgc ctcacgtatg cgaggtacac
     1861 ggcccccgaa tcgaacacac gctggtcgac cagacggagt gggagctcac tcccggcggg
     1921 gaagaacggt ttccccgagc cgaccaccac aggcaggaca accagctgga actcgtcgat
     1981 gaggtcgagc agagacgccg ccaggccggc cccgcccaga gcgagggggc cttcgatctc
     2041 gtccttcaga cggctgacca ccgccgccgc gtcccggctg cgaaccagct cgacaccgga
     2101 tgggacgctc tccagcgtat cggagaacac gaaacgcggc gtgccgacat aggcacgtgc
     2161 gaattctgcc tcgacgtcgg gtaggtcatc ctgcatcgca gccgcggtcc agtgctcgtc
     2221 catcacctcg aaaagccggc gcccgtacag ggacgcggct gccgcctcgg tacgttcatt
     2281 ccagtgccta tgcacgtcgt ctgcaggcgg ccctgagaac gcgatactcc cactggaatc
     2341 ctcgatatag ccgtcgattg acacattcat tccatagatg accttgccca ttctctgctc
     2401 ctttgtgtct acgcggtcag cgccctggtg atgtagaaga aaaccatccc caccaccaag
     2461 acgccccaga gattgcgccg gaagacgacg gccccgatta tcccacccag cgcggcagcg
     2521 agggccgggg tgacagcggg ctcaccttcc ggagccacga gcccagtgat ggcgatggca
     2581 acgaataggg cgaggggaaa gacatcgagg aaacggccca atgcgccttc cggcaccgaa
     2641 cgagggcgaa gcaggaaggt gactctcgtt ccgtaggtga tcgccgccac ggctaccact
     2701 acggccaggt cactcaccga cgaacacccc ggccatggac gccaccaccg cggccacggt
     2761 gatctgccaa gcggcgggaa ggacagcagt ggcgacgacg gaggccacca ccgctgcgcc
     2821 ggccacgagc aggtccttcc ggctcttgat catcggtgcg gcgaggccga ggaacagcaa
     2881 ggggaacaag actccgatcc cgaccgcttc tgtgtcgatg gagccgccga accaggcgcc
     2941 gatagtggtc ccgaccaccc agcccgagta gagcatgaga tcggccgccg tcttgtatgc
     3001 caccaggtct tcgacaccgg cgtcggctgc ccgaacggtg agaccgaaag tctcgtcgac
     3061 gaggatgaat gcgagccgca gcctggttcc aagggggcga ggcccgatca tgttggacaa
     3121 cgatgccgac atcgggatgt gacgcaaccc gagaccggtg gctgccagca gaacggcgac
     3181 cgggccggag gagaggagcc cgatcatggc gaactgcgcc gcaccggaaa cgatgatcac
     3241 cgatgagaag acggcgagcc atggcggaaa cccggcccgt accgccagga ctccgtaagc
     3301 cacgccaaac ccggcgacgg cgaggatgat gaggaccgag tcccgtatcc cctggcgaaa
     3361 tgacgggttg tcgagcaccc aggcacgcta atgactgccg gcccggctca aatcaccggc
     3421 ccggctcaaa tcaagggaag gtgcgcggca cccgctggac gttgatccag cgaagctcgg
     3481 tgaattcgtc gagggctgac tggcccccga atcgcccgta gccgctcccc ttgaccccgc
     3541 caaagggcac ttgaggctcg tcgttgacgg tctggtcccc gatgtggacc atcccggtct
     3601 cgagacgcat cgccatgtcc agagcccggt tgaagtcgct ggtgaggatc cccgacgaaa
     3661 gcccgtaatc cgaatcgttg gccacggcga gcgcctcctc ggcatcgtcc accacgacga
     3721 tcggcgccac cggcccgaat gtctgctccc tgaacaactg catgtctcgt gtgacgtccg
     3781 tgaccacggt cgggtgatag acgagattgt cgtgccggcc accagtgacc aggttggctc
     3841 cggcggccac cgcctcttcg acctgggtgt ggaccttcac gacggcgcgt tggttgatca
     3901 atgggccgat cgctgtggcc gggtcagtcg ggtcaccca
//
LOCUS       DASTXO010000373         2311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_759701, whole genome shotgun sequence.
ACCESSION   DASTXO010000373 DASTXO010000000
VERSION     DASTXO010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759701"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..89)
                     /locus_tag="VFU96_07825"
     CDS             complement(<1..89)
                     /locus_tag="VFU96_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEU5113214.1"
                     /translation="MARVLVLNASFEPLSVVTTRRAVVLVLNQ"
     gene            complement(117..725)
                     /locus_tag="VFU96_07830"
     CDS             complement(117..725)
                     /locus_tag="VFU96_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113215.1"
                     /translation="MASKDAGDLMKEKLVERLQDIPGVASVSLNLEDFESGIKVKLDE
                     DADEAEVLEAVRALLVAYGVRSLEDPTLEIGNRLSQARAALGVDVRISEVKGGARVEV
                     IGTAVRSFRLVPSTPMAIAQGVSDAWCQVLGRIPPEITRVALSDEGGLTVTATDGSQE
                     RIGHADIEEGWTMALPLAVGRAIGVLEPVSEAGEPSLAHTDW"
     gene            1023..2003
                     /locus_tag="VFU96_07835"
     CDS             1023..2003
                     /locus_tag="VFU96_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010987513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU5113216.1"
                     /translation="MAEVSAQQIEAFSRRFDLIATNVEKVIQGKRDVVELALLALVAE
                     GHVLVEDVPGVGKTQLAKSVARSIDSEFQRVQFTPDLLPSDVTGVSVWDRNRNEFEFK
                     PGPIFANIVVGDEINRASPKTQSALLEAMEERQVTSDGVTRALPLPFMVMATQNPLEH
                     EGTYPLPESQLDRFMMRVVIGYPSRQKELEILDTHGVRSTFLDLEPVVTVEEIEELIV
                     VAREVTVSSSVKSYIIDLVDGTRQHPDVLLGASPRSALFLQRLSRARAASQGREYVTP
                     DDIKMLAHPVLEHRMGLRPEAQMRGETLPDVIEDLMTRLRVPGTTSRIAT"
     gene            2003..>2311
                     /locus_tag="VFU96_07840"
     CDS             2003..>2311
                     /locus_tag="VFU96_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113217.1"
                     /translation="MPTLRGWALSGAGLALLVLWYALGEVELLLTGVFLLLAQAAALI
                     HVRRRRPSLEVSRRLGSSTVHDGETTTVTLLIENTSRHSVSRLAIEDDVNRLGVAVFE
                     V"
BASE COUNT          437 a          757 c          714 g          403 t
ORIGIN      
        1 ttctgattga ggacgaggac gactgcccgg cgtgtggtca ccaccgagag tggctcgaag
       61 ctggcgttca atacgaggac ccgtgccatg agaaacgctg ttcctgcccc gagacgttac
      121 caatcggtgt gggccaaact cggctcccca gcctcagaaa ccggttccag cacaccaatc
      181 gccctgccca ccgccagggg cagcgccatc gtccatcctt cttcgatgtc ggcgtggccg
      241 atccgctcct gactcccgtc ggttgccgtg acggtcaagc ccccttcatc ggaaagggcc
      301 accctggtga tctccggcgg gatacggccg agcacctgac accacgcgtc ggacacaccc
      361 tgggcaatgg ccattggtgt cgatgggacc aggcggaacg agcggacggc ggtgccgatg
      421 acctccaccc gggcgccacc tttgacctcg gagatccgga catcgacccc caacgcggct
      481 ctggcctggg agaggcggtt cccgatctcc agggttgggt cctccaggga ccgcacgccg
      541 taggcgacga ggagggctcg gaccgcctcg aggacttcgg cctcgtcagc atcctcgtcg
      601 agcttgacct tgatccccga ttcgaaatcc tcgagattca gcgagaccga agcaacgccg
      661 gggatgtcct ggagccgctc gaccagtttc tccttcatga gatcgcccgc atccttcgac
      721 gccacgacag ataagacgcc aaatcctccg gccggggttc cgccgctgac tggccgtagg
      781 ccgtgtgtat cctccagccc aggtacgcgt cggagggaaa cgcgccgtga cgccgtctca
      841 tggcaatcgc agccctgacc gcttcccaca acaaaatcgg cctgccagcc aaggcaaaca
      901 gacccctgcc cagtccgggc ccattgcgca tggatcggtt ggacactccc gtgggaactc
      961 tagctacgta taaaaccgtc tctcattaga gtcgaagcca ccaaagcgag acgggagggt
     1021 cattggccga ggtatccgcc cagcaaatcg aagcattctc gagacgattc gacctgatcg
     1081 ccaccaacgt cgagaaggtg atacagggaa agagggacgt cgtcgagttg gcactgctcg
     1141 ccctggtggc ggagggacac gtcctggtcg aggacgttcc cggagttggg aagacgcaat
     1201 tggccaagtc ggtggcccgg tcgatcgaca gcgagttcca gcgggtccag ttcaccccgg
     1261 atctcctgcc gtccgatgtc acaggcgtct cggtatggga ccggaaccgg aacgagttcg
     1321 agttcaagcc gggcccgatc ttcgccaaca tcgttgtcgg cgacgagatc aacagggcca
     1381 gccccaagac gcaatctgcc ctgctcgaag cgatggagga gcgacaggtc acatctgatg
     1441 gcgtgacgcg tgctctgccg ctcccgttca tggtcatggc gactcaaaac ccgctggagc
     1501 acgaggggac ctatcccctg cccgaatcgc agctggacag gttcatgatg agggtggtca
     1561 tcggctatcc aagccggcag aaggaattgg agatcctcga cacgcatggc gttcgctcca
     1621 ccttcctcga cctggagccc gtggtaacgg tcgaggagat cgaggagctc atcgtggtgg
     1681 ctcgtgaggt gacggtttcc tcgtcggtga agagctacat catcgacctg gtcgatggga
     1741 cccgccagca tcccgacgtg ctgctcgggg cctccccccg ctcggccctc ttcctgcaac
     1801 gtctgtcgcg agctcgtgcc gcctcgcagg gcagagagta cgtcacaccc gacgacatca
     1861 agatgctggc ccaccccgtg ctggagcatc ggatggggct gcggcccgag gcccagatgc
     1921 gaggcgagac gctccccgac gtcatcgagg atctgatgac caggctgaga gtgccgggca
     1981 ccacgtcccg gatcgctacc tgatgccgac cctgcggggc tgggcccttt ccggggccgg
     2041 tctcgccctt ctcgtcctct ggtacgccct gggcgaggtc gaattgcttc tgactggcgt
     2101 cttcctcctg ctggcccagg cagctgccct gatacacgtg cggagacgac ggccaagtct
     2161 ggaggtcagc cggagactgg gctcgtcgac cgttcacgac ggagagacca cgacggtgac
     2221 cctcctgatc gagaacacct cacgacacag tgtcagccgt ctagccatcg aggacgacgt
     2281 gaaccgtctc ggagtggcgg tcttcgaggt g
//
LOCUS       DASTXO010000374         3270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_575524, whole genome shotgun sequence.
ACCESSION   DASTXO010000374 DASTXO010000000
VERSION     DASTXO010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575524"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..444
                     /locus_tag="VFU96_07845"
     CDS             46..444
                     /locus_tag="VFU96_07845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU5113218.1"
                     /translation="MPHQQQPVEMILLRQLASYLAIPMWMMDDTGNLIYYNEAAEDLL
                     GIRFDDVGPITSENLVGAWKVTQLDGSALPEYDFPVVTALSKRKPAHRAIRFQGMDGV
                     WRAVEVTALPIEGQGERFLGVLATFWEVED"
     gene            531..1268
                     /locus_tag="VFU96_07850"
     CDS             531..1268
                     /locus_tag="VFU96_07850"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5113219.1"
                     /translation="MVGNEGRLIVLDAGSGMRALGANVEAAEQVDVLITHLHMDHIQG
                     LPFFAPLHDPDVRVDVWGPISTTQTLSERLNRYLSPPLFPLRVRDLSNVVFHDVPPGT
                     FEIDGIQVTADLVSHPGPTLGYRLEENGKVLTYLPDHEPALGMHNFPKNPEWTSGYGL
                     ASGADLLIHDSQYTDEEYSERVGWGHSSYSHLLAFAAMTGCRTLMTFHHDPDHSDQKL
                     DEAHERLRATGPEFEIVAGTHGAVLDV"
     gene            1273..2307
                     /locus_tag="VFU96_07855"
     CDS             1273..2307
                     /locus_tag="VFU96_07855"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5113220.1"
                     /translation="MTILFIVIDGLGSRSVTPQVMPTLTDWGHRGLIRPKGATSVMCS
                     STYPNFASIVTGRLATEHGIMTNQVVVGGVARPASEIGPRTETFLDHDCEVVVGDQHL
                     IGVMAAPTAGRHWPPDGIVPGGVVLDLFGYVTDNEVATRVLDALDRRPALIFAQLNSP
                     DTFAHVHGPDSEEAIESYRALDSTLDVIGSAMRWDRDLVLVTSDHDQETVDPSARIDL
                     QGVATDRGLDVTIVDEGTAAMLAGPSAVQPTWIEGIPGIAGWDQVASDVVLVSSIPGW
                     WFADADLPDLRGAHGGPRTRATVAVAAGAEAQLQPIADRFLGDRLGAEDWFDLVQIAR
                     DTSLNVESGT"
     gene            2317..2676
                     /locus_tag="VFU96_07860"
     CDS             2317..2676
                     /locus_tag="VFU96_07860"
                     /inference="COORDINATES: protein motif:HMM:NF021525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2255 family protein"
                     /protein_id="HEU5113221.1"
                     /translation="MSLTAEQLQEIGDSQLVIIETRRGDRVTGTVIWVVVVDDVVYVR
                     SVRGESGRWYQRAVADAAVALRVGDDRFEFRAIPANDPDSVESASEGLRRKYRGRSLE
                     NMLLPETLGTTLRLEPR"
     gene            complement(2691..>3270)
                     /locus_tag="VFU96_07865"
     CDS             complement(2691..>3270)
                     /locus_tag="VFU96_07865"
                     /inference="COORDINATES: protein motif:HMM:NF016196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HEU5113222.1"
                     /translation="AGELVFERIIASFRLLVDEVASDPSHPIRADIEMMLSRIADDLR
                     NSADLAERIEESKLHILDSPELAAAVQTQWQVVQESLGRAASQPGSDLEKRLSELIQW
                     WANRVLEDEALRMRIDTWIAEAAERLAHQWDDEVIGMIETTVAGWDATEISHRLELQL
                     GRDLQFVRINGTVVGALVGIAIHALLVLTGNV"
BASE COUNT          585 a         1040 c         1050 g          595 t
ORIGIN      
        1 ctaccgagat gacagtcgag cgtgggtggc ggtaggttct cccccatgcc gcatcagcaa
       61 cagcctgtgg agatgatcct cctgaggcag ctcgccagtt atctggccat cccgatgtgg
      121 atgatggatg acacggggaa cctgatctat tacaacgaag cggccgagga cctgctcggc
      181 atccggttcg atgatgtcgg cccgatcacc tcggagaacc tcgtcggcgc ctggaaggtc
      241 acacagctcg acgggtcggc cctgcccgag tacgactttc cagtcgtgac ggccctcagc
      301 aagagaaaac cggcccacag ggcgatccgg ttccagggga tggacggagt gtggcgggcg
      361 gtggaggtga ctgctctccc gatcgagggt cagggggaga ggttcctcgg cgtcctcgcc
      421 acgttctggg aggtggagga ctgagggcca aactgtgggg aacgcgcggg tcgatcgcca
      481 gcgccgggtc ggagacggcc aaatacggtg gcgacacatc cgcggtcgag gtggtcggga
      541 acgaggggag gctcatcgtc ctcgacgccg gtagcgggat gcgggcgctg ggggcaaatg
      601 tcgaagctgc agagcaggtc gacgtgctca tcacccacct ccacatggac cacatccagg
      661 ggttgccctt cttcgcccca ttgcacgacc ccgacgtccg tgtcgacgta tggggaccga
      721 tctcgacgac tcagacgctt tcggagcggc tcaaccgcta tctgtccccg cccctgttcc
      781 ctttgcgtgt gcgagatctc tccaacgtcg tctttcacga tgtcccacca ggcacgttcg
      841 aaatcgatgg gatccaggtc acagccgatc tggtctccca tcccggcccc accctcggct
      901 accgactcga ggagaacggg aaggttctca cctatctccc tgatcacgag cctgcgctgg
      961 gaatgcacaa cttccccaag aacccggagt ggacgtctgg ctacggtctg gcatccgggg
     1021 cggacctcct gatccacgat tctcagtaca ccgacgagga gtattcagag cgtgtggggt
     1081 gggggcacag ttcctacagc catctgctcg ctttcgcggc gatgacgggg tgtcggaccc
     1141 tgatgacgtt ccaccacgac cccgatcaca gcgatcaaaa gctcgacgag gcgcacgaac
     1201 ggcttcgggc aacgggtccg gagttcgaga tcgtcgccgg cacccacgga gcggtcctgg
     1261 acgtatgacg aggtgacgat cctcttcatc gtcatcgacg gcctcgggtc tcgctcggtc
     1321 acgccccagg tcatgccgac tttgactgat tgggggcacc gcggcctgat tcggccaaag
     1381 ggcgccacca gcgtcatgtg ctccagcacg tatcccaact tcgccagcat cgtcaccggc
     1441 aggctcgcca ccgaacacgg gataatgacc aatcaggtgg tggtcggagg cgtcgccaga
     1501 ccagcgtccg agatcggccc tcgcaccgaa acctttctcg accacgactg cgaggtggtt
     1561 gtcggcgacc agcatctgat cggagtgatg gcagctccga cggctggtcg gcattggcct
     1621 cctgacggca tcgttcccgg tggggtcgtg ttggatctgt tcggatacgt gactgacaac
     1681 gaagtggcga cgagagtcct cgacgctctc gatcgcaggc ccgcgttgat cttcgcccag
     1741 ctgaacagcc ccgacacctt cgcccatgtg cacgggcctg actcagagga ggccatcgag
     1801 tcttaccggg cgctcgattc gaccctcgac gtcatcggat cggcgatgcg ctgggaccgc
     1861 gacctggtcc tggtcacatc ggaccatgac caggagacag tagacccgag tgcacgaatc
     1921 gacctgcagg gagtcgccac ggaccgcggc ctcgacgtca ccatcgtgga tgagggaacc
     1981 gccgcgatgc tcgctgggcc ttcggctgtc cagccgacct ggatcgaagg gatcccgggt
     2041 attgccgggt gggatcaggt ggcctctgac gtcgttctcg tctcgtccat tccaggctgg
     2101 tggttcgccg acgccgacct tcccgacctc cggggcgccc acggcgggcc ccgaaccagg
     2161 gcgacggtgg ccgtcgcggc cggcgccgaa gcgcagctcc aacccatcgc tgaccgtttc
     2221 ctgggtgatc ggttgggggc tgaggactgg ttcgacctgg tccagattgc tcgcgacacc
     2281 tcgctcaacg tggagtccgg cacgtagtct cggatgatgt cgttgaccgc agagcaactt
     2341 caggagattg gggactcgca gctggtcatc atcgaaacca ggagaggcga ccgggtgacc
     2401 ggcacggtga tctgggtcgt tgtcgtcgat gatgtcgtct atgtgcgctc ggtccgaggg
     2461 gagtcgggac gttggtacca aagggcggta gccgacgcgg ctgtggcgct cagggtcggg
     2521 gatgaccgct tcgagttcag agcgattccg gcgaacgacc ccgattcggt cgaatcggct
     2581 tccgaagggc tccgccgcaa gtatcggggc cgctcactcg agaacatgct cctccccgag
     2641 actctgggca ccactctgcg tctcgagccc cggtagccgt ccgcagacgc tcacacgttt
     2701 ccggtgagca cgagaagagc gtggatagcg atcccgacca gggcccccac gacggttccg
     2761 tttatccgca cgaactggag gtccctgccc agctggagct cgaggcggtg agagatctcg
     2821 gtggcatccc acccggccac tgttgtctcg atcatcccga tgacctcgtc atcccattga
     2881 tgggcgagcc gctctgcggc ttcggcgatc caggtatcga tgcgcatgcg tagagcctcg
     2941 tcctcgagca cgcggttggc ccaccactgg atcaactccg acaggcgctt ttccaagtca
     3001 gaacctggct gagacgctgc ccggcccaga ctctcctgga caacctgcca ctgagtctgg
     3061 accgccgcgg ccaactccgg tgagtccagg atgtgcagct tcgattcctc tatgcgctcg
     3121 gccagatcag cagagttgcg caggtcgtcg gcgatccgcg agagcatcat ctcgatgtcg
     3181 gcgcggatcg gatgtgacgg atcggacgcg acctcgtcga cgagtagccg gaaggaggca
     3241 atgatccgct cgaagacgag ctctcccgcc
//
LOCUS       DASTXO010000375         2555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_980519, whole genome shotgun sequence.
ACCESSION   DASTXO010000375 DASTXO010000000
VERSION     DASTXO010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980519"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VFU96_07870"
     CDS             <1..673
                     /locus_tag="VFU96_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113223.1"
                     /translation="NGDSYNIESCTSLGTCSSIISLIMVLVGVVSAITSLFFRWFKSQ
                     GVERLQMKWLVLAFVIFMIGVLAEFGGFQYSIVAEIFLPVGLFLIPITIVIAVTRYRL
                     YEIDRIISRTVAYVVVIALLGAAYFLGLTAMTNWLPSDSPLAVAASTLAVAALFNPVR
                     KRVQALVDRRFNRSRFDAQKVMDGFSGSLRDQMDPNGVIEGWVGVVSETMQPASAAVW
                     VREHR"
     gene            670..1710
                     /locus_tag="VFU96_07875"
     CDS             670..1710
                     /locus_tag="VFU96_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113224.1"
                     /translation="MKARLVTVLPWVLLIMTTVTWGLAFWFDRGSEEVTGLLDKAWAL
                     SFFAFPIVGVFLARRIPSNAVGWLFTAGPLLVGTGVALQEMAETLDLPSLVTPSEYVF
                     DAGLLTLFSSLTLFPDGRYRRPWWPWAHGIAIVVLSLVAGSFIAVILILGLLPLVYRL
                     IKGSPLVRRQIAGPIVVLILGVVSLVFVNVAFGGTALAQTLITALTMLITIGVPVSIA
                     VAITRYRLYEIDRLVSRTVSYLLVIVMLGAVFVGVVTMAGSLLETESDLAIAASTLAV
                     AALFNPLRKRVQGWVDRRFNRSRYDAQKVMDGFSGSLRDQVDPGRVIEGWVGVVSDTM
                     QPASAGVWVRER"
     gene            1707..>2555
                     /locus_tag="VFU96_07880"
     CDS             1707..>2555
                     /locus_tag="VFU96_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113225.1"
                     /translation="MSRRLLKAATVVWVILMVIAAVIWLTFPGDIDSMVGILFASVCS
                     VVVGAILTLKVPSNSIGPLALIAGSAWVLYLFGSGYGAASLASSDALPLAYFFGWLGA
                     WTGAFFPIGMSALILCFPTGKPVSRWRVVGLGPLVAALSTVAGAVLLWGIPLEMFQTE
                     VVSQSRWYELVDAGFIIGFVSVIPATLSVVARYRRAESVERQQIKWLLAATTLLALAF
                     IAGAFSDDSNETAWWIVSLAMAAIPISILFAVLRYRLYDIDRILSRTVSYVIVIGLLA
                     GVYLLGL"
BASE COUNT          347 a          759 c          808 g          641 t
ORIGIN      
        1 gaacggcgac agttacaaca tcgagtcctg cacttccctg ggcacctgct cgagcatcat
       61 cagcctcatc atggtgttgg tcggggtcgt gtccgcgatc acctcactct tctttcggtg
      121 gttcaagtct cagggcgtgg agcgacttca gatgaagtgg ctggtcctcg ccttcgtgat
      181 cttcatgatc ggcgtcctcg ccgagttcgg cggcttccag tactcgatcg tggccgagat
      241 cttcttgccg gtcgggctat tcctgatccc gatcacgatc gtcatagccg tcaccagata
      301 ccgactgtat gaaatcgatc ggatcatctc ccgcaccgtc gcttacgtag tcgtgatcgc
      361 gctgctcggc gcggcctact tccttggtct gacggcgatg actaactggc tgccctccga
      421 ttcaccgctg gcggtcgccg cctcgacgct ggcagttgct gccctgttca atccggtccg
      481 gaagcgggtg caggcgttgg tcgaccgccg gttcaaccgc tcccgcttcg atgctcagaa
      541 ggtgatggat gggttttccg gctcgttgcg ggaccagatg gacccgaatg gggtaatcga
      601 aggctgggtt ggcgtcgtgt cggagacgat gcagcctgcg tcggctgcag tatgggttag
      661 ggagcaccga tgaaggccag actcgtcacg gtccttccct gggtacttct gatcatgacc
      721 accgtcacct ggggcctggc gttctggttc gatcgcggct ctgaggaagt gaccgggctt
      781 ctcgacaagg cttgggccct ctccttcttt gctttcccaa tcgtcggggt gttcctcgcc
      841 cgccggatcc cgtctaatgc cgttggctgg ctcttcaccg ccggcccatt gctggtgggg
      901 acgggagtcg ccctccagga gatggctgag actctcgacc tcccgagctt ggtgacgccg
      961 tccgagtatg tcttcgatgc gggtttgctc acattgtttt cgtccttgac gcttttcccc
     1021 gacgggcgct atcgacggcc gtggtggccg tgggcccacg gaattgccat cgtggtcctg
     1081 agcctggtgg cgggcagctt tattgcagtg atcctgattc tgggtctctt gcccctggtc
     1141 tatcgattga tcaagggctc gccgctggtt cgccgtcaga tcgccggtcc gatcgtggtg
     1201 ctgatacttg gtgtcgtttc actggtattt gtgaacgttg ccttcggtgg cactgctctc
     1261 gcccagacct tgatcaccgc gttgaccatg ctcatcacca ttggtgtccc ggtgtcgatt
     1321 gccgttgcga tcacccgata tcgcctctac gagatcgacc gactcgtgtc cagaaccgtc
     1381 agctacctgt tggtgatcgt gatgttgggt gcggtcttcg tcggtgtggt gacgatggcc
     1441 ggctcgttgc tggagacgga gagcgatttg gcgatcgctg cctccactct ggccgtggcc
     1501 gcgctgttca atccgctacg gaagcgggtc caaggttggg tcgaccgccg gttcaatcga
     1561 tcgcgttacg acgctcagaa agtgatggac ggcttttcgg ggtcactgcg ggatcaggtg
     1621 gaccctggtc gtgtcatcga gggctgggtc ggtgtggtct ccgacacgat gcagcccgct
     1681 tcggcgggag tgtgggtgag ggagagatga gccggaggct cctcaaagcc gcgaccgtgg
     1741 tgtgggtcat cctgatggtc atcgcggccg tcatctggct gacgtttccc ggagatatcg
     1801 actccatggt cgggatcctc ttcgcttcgg tgtgctcggt cgtcgtcggg gcgatcctga
     1861 ccctcaaagt gccgtcgaat tcgatcgggc ccctggctct catcgccggc tcggcctggg
     1921 tcctctacct gtttggcagt gggtacgggg cggcctcgct tgcctcgtcc gatgccctgc
     1981 cccttgctta ctttttcggg tggctggggg cctggactgg agcgttcttc ccgatcggaa
     2041 tgtcggcctt gatcctgtgc ttcccgaccg gcaaacccgt ttctcggtgg cgtgtcgttg
     2101 gcttggggcc attggttgcg gcgctttcga ccgtggccgg ggcggtcctc ctttggggga
     2161 tcccgctcga gatgttccag accgaggtgg tgtcccaatc gaggtggtac gagctcgttg
     2221 atgctggctt catcatcgga ttcgtctccg tgatcccggc cacattgtcg gtggttgctc
     2281 ggtatcggag ggcagagtcg gtggaacgcc agcagatcaa gtggttgctg gccgcaacca
     2341 ccctcctcgc gctggccttc attgcgggcg cgttctccga cgactccaac gaaacggcct
     2401 ggtggatcgt ctccctggcg atggccgcca tcccgatctc gatcctgttt gccgttctgc
     2461 gatatcgcct ttacgacatc gatcggatcc tgtcacggac cgtgtcatat gtcatcgtga
     2521 tcggccttct tgccggggtg tatctgctcg gactc
//
LOCUS       DASTXO010000376         2442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1128222, whole genome shotgun sequence.
ACCESSION   DASTXO010000376 DASTXO010000000
VERSION     DASTXO010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128222"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..139)
                     /locus_tag="VFU96_07885"
     CDS             complement(50..139)
                     /locus_tag="VFU96_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HEU5113226.1"
                     /translation="MGSLVKKRRKKMSKHKYRKRLKANRHKRK"
     gene            complement(224..985)
                     /locus_tag="VFU96_07890"
     CDS             complement(224..985)
                     /locus_tag="VFU96_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113227.1"
                     /translation="MNRPIEQTLLELAHHIDWPEPSDQGVDLRSRLAAAPARRARRRW
                     IPATAIVLIMVASLLLFSPRARQAVADLLGVAGIEIRFDPDPAKIVGGELDLGEAMTL
                     EEAAEAVDFELTVPANLPPPDGVFLSDRPSSGRVSMVWEGTEALPASGESGIGVVYSQ
                     FALELAEDADFVKSVMPDTSVRAVEVDGAIGLWIEGAPHVISYEDAAGNRVEEETRLA
                     GNVLMWESQGVTHRIETTLGLGATLALAGSLQPVG"
     gene            complement(978..1553)
                     /locus_tag="VFU96_07895"
     CDS             complement(978..1553)
                     /locus_tag="VFU96_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU5113228.1"
                     /translation="MEGRPLNEQDLIERAKSGDVAAFEALVQIHRPIALRVAFLVVGD
                     RTEAEDVTQEAFVKAYRAMSRFQVGAPFRPWLLRIVRNEALNQRRRRGRQERLSLRLA
                     NDPVSGGAAPSPETTVLTGVERTTVLDAVGALPDRYRDVITHRYLLGLTEAETSATLR
                     IPVGTVKSRTARALDRLEAALSGLSLGGGGE"
     gene            1668..2180
                     /locus_tag="VFU96_07900"
     CDS             1668..2180
                     /locus_tag="VFU96_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113229.1"
                     /translation="MKRLTRVLIPTTLLVLLLAPTALAGSVAVVTFAELPSDFDAGTP
                     YTLDYSILAHGIEPMPFSETSLHFQGPNGEDLTFPAESTGDGRWTAEVTLPESGEWQW
                     EVLAGDQVIQPLGMMPVLPAPASIPTGLINSLRIALPIATLLALVLLVDQIRTRPRSE
                     RQPSTAPDAV"
     gene            2197..>2442
                     /locus_tag="VFU96_07905"
     CDS             2197..>2442
                     /locus_tag="VFU96_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU5113230.1"
                     /translation="MTAVIVALLAGATVAVYALDPDAQPEAAEAGPIEGRTVFLTKGC
                     TGCHSREGASDTGFAGPDLTGLSDRAGDRVEGRSAEEY"
BASE COUNT          415 a          841 c          757 g          429 t
ORIGIN      
        1 ttgaagggcc aatagcgaag gaccggctga cgccggtcct ttcacgaagt cacttccgct
       61 tgtggcgatt cgccttcaga cgcttgcggt atttgtgctt cgacatcttc ttgcgacgct
      121 tcttgaccaa cgatcccatg caaaaacctc ggttccgggt ggggagacga cacatggtcg
      181 caccgatccg tcccctattc aaaccggatc cgcaggtcga gcgtcatccc accggctgca
      241 acgagcccgc cagcgccaac gtcgcaccca agcccaatgt cgtctcgatg cgatgggtga
      301 caccctggct ctcccacatc agcacgttgc cggccagcct cgtctcctcc tcgacgcggt
      361 tgccggctgc gtcctcgtag gagatgacgt gtggcgctcc ttcgatccaa agcccgattg
      421 cgccgtcgac ctccaccgcc cgaaccgacg tgtccggcat gacgctcttg acgaagtctg
      481 cgtcctcggc caactccagc gcgaactggc tgtagaccac cccgattcct gactcgccgg
      541 aggcggggag cgcctcggtg ccttcccaca ccatggagac ccgaccggac gagggccggt
      601 cggagaggaa cacgccgtcg ggtgggggca gattcgccgg gaccgtcaac tcgaagtcca
      661 cagcctcagc tgcctcctcg agggtcatcg cttcgcccag atcgagctcc ccgcccacga
      721 ttttggcggg atcgggatcg aagcggatct cgatcccggc cactccgagc aggtcggcaa
      781 ccgcctgtcg cgcccggggt gagaagagaa gcaatgacgc caccatgatg aggacgatgg
      841 ccgtcgccgg gatccagcgt cggcgagccc gccgagcagg ggcggcggcc aggcggctgc
      901 gcaggtcaac accctgatcc gacggctccg gccagtcgat gtgatgtgcg agttcgagga
      961 gggtctgctc gatcggccta ttcaccgccg cctcccaggc tgagacccga cagcgcagct
     1021 tcgaggcgat cgagggcgcg cgcggtccgc gacttgaccg tccccaccgg gatccgcaaa
     1081 gtcgccgacg tctcggcctc ggtgagaccg agcagatagc gatgcgtgat cacatcccgg
     1141 tatcgatcgg gcagtgcgcc gaccgcatcg agcactgtcg tcctttcgac gccggtgagg
     1201 accgtcgtct cgggagatgg ggccgcacct cccgagacgg ggtcgttggc caggcgcagg
     1261 ctgagccgtt cctgacgtcc gcggcgacgt cgctgattca gggcctcgtt gcggacgatc
     1321 cgcaatagcc agggtcggaa tggggcgcct acctggaatc gcgacatggc gcgatatgcc
     1381 ttgacgaacg cttcctgagt cacgtcctcg gcctcggtcc ggtctcccac gaccaggaaa
     1441 gccacccgca gcgcgattgg ccgatggatc tgcaccaagg cttcgaacgc agcgacatcg
     1501 ccactctttg ctctctcgat caggtcttgt tcgttcagtg ggcggccctc caccgacgct
     1561 ccgtccaatg cctacaccga ccgggccgga tcggttccct cgaatttcat ggaacccggg
     1621 gcatgcatcc ggtgtatgga ctgatgacca cgatccgagg agatcacatg aagcgcctga
     1681 cccgggtact catacccacc actctcctgg ttctgctgct ggccccgacc gccctcgccg
     1741 gctcggttgc ggtggtcacg tttgcagagc tcccttccga tttcgatgcc ggcaccccct
     1801 acaccctcga ttactcgatt ctggcccacg gcatcgagcc gatgcccttc agtgagacat
     1861 ccctccactt tcaagggccc aacggcgagg acctgacctt tccggccgaa tcgaccggag
     1921 atggccggtg gacagccgag gtgaccctcc ccgaatcggg agagtggcaa tgggaagttc
     1981 ttgcaggaga tcaggtcatc cagccgctgg gcatgatgcc ggtcctcccc gctccggcgt
     2041 cgattcccac cgggctgatc aactcactcc ggatcgcctt gcccatcgcc accctgctgg
     2101 ccctggtgct gctggtcgac cagatcagaa cacgacctcg cagcgagcgg cagccgtcga
     2161 cagcaccaga tgcggtctag tggtgccagt ctgctggtga cggccgtcat cgtggccctg
     2221 cttgccgggg ccacggtggc ggtctacgcc ttggacccgg atgcgcagcc ggaggcggcc
     2281 gaagcaggcc cgatcgaagg ccgcacggtc ttcctcacca agggctgcac gggatgtcac
     2341 agccgggagg gtgcctccga taccggtttc gccgggcccg acctcacggg tctctccgat
     2401 cgggcgggtg atcgggtgga ggggcgctcc gccgaagagt ac
//
LOCUS       DASTXO010000377         4507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_539164, whole genome shotgun sequence.
ACCESSION   DASTXO010000377 DASTXO010000000
VERSION     DASTXO010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539164"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VFU96_07910"
     CDS             <1..588
                     /locus_tag="VFU96_07910"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5113231.1"
                     /translation="FAFPALTRQPEAFNLGARCVNPDITFTQQYIETWTDTGIAKSAT
                     QAEIAGGADVVLSATDSAVFGILEGAAEADGQVWVVPSYYESQSLDPDHVMTSAIHGL
                     TFASKSIIESGAAGEIGESAFIDFDATNDPEIDAPLYDNIASMLDADQLALYEDILAK
                     VRSGEIAIPDETTGDPTIGTEGSGAEIDPASIGCG"
     gene            628..1431
                     /locus_tag="VFU96_07915"
     CDS             628..1431
                     /locus_tag="VFU96_07915"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEU5113232.1"
                     /translation="MKTDMYCYMENRNKLTLPSGVPGYSDAFESWLDPAHTAVLEIDM
                     HRGHVGEDDRLTLAVPRAKERVPAHNRFQAECRSVGIPIIHVQHWQRHGGIDDLNNRK
                     HNRMANWRVLYYLYVDDNEMMDEHSWEGTKWLDLITEEEEGDYYIRTKKRLSAFYPTD
                     LEFLLQNLDIHNVVLTGTMTDACVLSTAFDAANRDFRVIVPRDVVGGFSEEAEHGALQ
                     VISLHLGLVVDAPALLAEYFARAGLELPSDLVGVSDINEVTGLLEKSPV"
     gene            1481..2854
                     /locus_tag="VFU96_07920"
     CDS             1481..2854
                     /locus_tag="VFU96_07920"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU5113233.1"
                     /translation="MASDRKAIWGDEPKPLMPYSPAIKAGGWVFFAGQLASDFKDGLA
                     PEAGGDGRNPNVDNQLELQSRFVLQNLKKTAAAAGIDLEKDIVRIYQWFTSPFPTMEE
                     FADGNTWPRISITPYLDTRNEFIFEPRPASTGMGIRETGLLVKDTILEVDMIGMDPAV
                     VPGPSTGTAVPEGTPSPLAGYSPAIERGDWVFLAGEIPVDWVGDWGRPEYYGTPSGVA
                     LAARTNPNFWYDSDIERQTDYTLEKQAAIAEAAGTSLDRAVKATVYIGHPSEFEGMDK
                     VWKRWFPENPPARTVIPYMGLGGRGSRVEVAFKLLSGKSELSVETIEAPGVPPGLGHE
                     PQAVKAGKFLFFSTVAPFDHMGTLEESTKRHPSFPFYGQPPKLEMRYMLDKAAKICEA
                     AGTSLDQICRRQAFHDDFTHFAQSIEEWAAHFDGDKPASTTLEIGGPLLVPGSHVLLD
                     LIGYCPD"
     gene            2873..4069
                     /locus_tag="VFU96_07925"
     CDS             2873..4069
                     /locus_tag="VFU96_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEU5113234.1"
                     /translation="MTVPTPYWAPDDFEQVNGFPRYPVSEMERRHRWVADTMSEHELD
                     VVIVGGPTGPLETSVQYFSNWPSQVQSYVLFVPGEAPLLVLRLWNHVPDAERIAMIDD
                     VRYGGDTPREQGEKVATFASEHNARRIGMIGVVPHADLAMIQQSNPEAILVDLNEAYQ
                     RFRLVKSPAEMVFVNIASRMNDAAIDSLAAQLRPGVNEYEVARIVEDVYLANRGWNLI
                     HFSLSTPMAAPEFCVPHQYHPDRTLQAGDVFVTEISTTFWGYAGQILRTFTIASDPTP
                     RYERLHEVALDAYQSIVEVLRPGATIGEVLDAAEVITESGFDIWDDLVHGFGGAYLPP
                     IIRTRANRGATHPEEFAYPDGTLLVVQPNVIDGPAGVQAGNSLQITPDGAVSTQRQPT
                     TMIRCA"
     gene            4080..>4507
                     /locus_tag="VFU96_07930"
     CDS             4080..>4507
                     /locus_tag="VFU96_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5113235.1"
                     /translation="MSGSPAVEMRGISKAFGDTVANDSVDFSVRSGDIHVLLGENGAG
                     KTTLMNILFGHILPDAGVILVDGEEVEISAPNDAIAHGIGMVHQHFSLVPSFTVAENV
                     MMGVNRVTDLSFSDDAIEQAVADRAEELHMPIKPDATVEVL"
BASE COUNT          892 a         1489 c         1359 g          767 t
ORIGIN      
        1 ttcgccttcc ccgccctgac ccgtcagccc gaggcattca acctcggagc gaggtgtgtc
       61 aaccccgata tcaccttcac ccagcagtac atcgagacct ggaccgacac cggcatcgcc
      121 aagtcggcga ctcaggcgga gatcgcgggt ggagcagacg tagtcctgtc ggctaccgac
      181 tcggcggtgt tcgggatact cgagggtgcc gcggaggccg acggccaggt gtgggtggtg
      241 ccgtcctact acgagagcca gtcactcgac ccggatcatg tgatgacctc ggcgattcat
      301 ggattgacct tcgcctccaa gtcgatcatc gaatccggcg ccgccggtga gatcggtgaa
      361 agcgcattca tcgacttcga cgccaccaac gatccagaga tcgacgcgcc tctgtacgac
      421 aacatcgcct cgatgttgga cgcggatcag ctggccctct acgaagacat cctggccaag
      481 gtgcgctccg gtgagatcgc catccccgac gagaccacgg gcgatcccac tatcggcaca
      541 gaggggtccg gagcagaaat cgacccggcg tccatcgggt gcggatgatg acatgaccca
      601 gaggagaccc gacgaggagg tggaagcatg aagacggaca tgtattgcta catggagaat
      661 cggaacaaac tgaccctccc gtcgggtgtc cccggctatt cggacgcatt cgagagctgg
      721 ctcgaccccg cgcacaccgc ggtcctcgag atcgatatgc accgcgggca tgttggagag
      781 gacgatcggt tgaccttggc ggtgccacgt gccaaagagc gtgtccccgc ccacaaccgc
      841 ttccaggccg agtgccgaag tgtcggcatt ccgatcatcc acgtccagca ctggcagcgg
      901 cacgggggga tcgacgatct caacaaccgc aagcacaacc ggatggcgaa ttggcgggtc
      961 ctctactacc tctacgtcga cgacaacgag atgatggacg agcactcctg ggagggcacg
     1021 aagtggctcg acctgatcac tgaagaagaa gagggcgact actacatccg caccaagaag
     1081 cggttgtcgg cgttctatcc caccgacctg gagtttctcc ttcagaacct cgatatccac
     1141 aacgtggtgc tcacgggcac gatgaccgac gcctgcgtgc tgtccaccgc cttcgatgcc
     1201 gccaaccgcg acttccgggt catcgtgccc agggacgtcg tcggtggttt ttcggaggag
     1261 gcggaacacg gcgcactcca ggtgatctca ctccatctcg gtttggtggt ggacgcgccg
     1321 gctctgctcg cggagtactt cgcgcgggct ggcctcgaac tcccatcgga tctagttggg
     1381 gtcagcgaca tcaacgaggt caccggcctg ctcgagaagt ccccggtcta gaccactcca
     1441 ggcccgaccc ccgagactcc caccgaagag aggaaacacc atggcaagtg accgcaaggc
     1501 gatctggggc gacgagccca agccgttgat gccctacagc ccggcgataa aggccggcgg
     1561 atgggtgttc ttcgccggcc aactggcttc tgacttcaag gacggtctgg ctcccgaagc
     1621 cggtggtgac gggcgcaacc ccaatgtcga caaccagctc gagttgcagt cacgattcgt
     1681 gttgcagaac ctcaaaaaga ccgccgccgc ggccggcatc gacctcgaaa aagacatcgt
     1741 ccggatctat cagtggttca cctcaccgtt cccgacgatg gaagagttcg ccgacgggaa
     1801 cacctggccc cgcatctcga tcactccgta tctggacacg cgaaacgagt tcatcttcga
     1861 accgcggccc gcctccaccg gaatgggaat ccgcgagacc ggtctcctgg tgaaagacac
     1921 gattctcgaa gtcgacatga tcggaatgga tcctgccgtc gtgcccgggc cgagcaccgg
     1981 gaccgccgtc cccgaaggga caccctcccc cctcgccggc tactcccccg ccatcgaacg
     2041 cggtgactgg gtgttcctcg ccggggagat cccggtcgac tgggtcggag actggggccg
     2101 ccccgagtat tacgggaccc ccagcggcgt cgccctcgct gccaggacca accccaactt
     2161 ctggtacgac tccgacatcg agcgtcagac cgactacacg ttggagaagc aagcagccat
     2221 cgccgaggcg gcaggcacgt ccctggaccg agccgtcaag gcaaccgtct acatcgggca
     2281 tccgtccgaa ttcgagggca tggacaaggt ctggaaacgc tggttcccgg agaatccccc
     2341 ggcccgtacg gtcatcccct acatgggatt gggtggccgt ggctcccgcg tcgaggtggc
     2401 gttcaagctg ctctccggca agtccgagct ttcggtcgag acgatcgagg cgcccggcgt
     2461 cccacccggc ctcggccacg aaccgcaggc ggtgaaggca gggaagttcc tcttcttctc
     2521 gaccgttgcg ccgttcgatc acatggggac gctggaggag tcgacaaaac gccatcccag
     2581 cttccccttc tacgggcagc caccgaagct ggagatgagg tacatgctcg acaaggccgc
     2641 caagatctgt gaggcggccg gcacgtcgct cgaccagatc tgccgtcgcc aggctttcca
     2701 cgacgacttc actcatttcg cccagtcgat cgaggaatgg gcagcccatt tcgatggcga
     2761 caagccggcc tcgacgaccc tcgagatcgg cgggccgctg ttggtgccgg gctctcatgt
     2821 gctcttggac ctcatcggct actgccccga ctgaccgccg gggaggccgc agttgacggt
     2881 tccaacgccc tactgggcgc ctgatgactt cgagcaagtc aacggctttc cccgctaccc
     2941 ggtgtccgag atggaacgtc ggcatcgctg ggtcgccgac acgatgagcg agcacgagct
     3001 cgacgtcgtc atcgtcggtg ggcccaccgg cccgctggag acgtcagtcc aatacttcag
     3061 caattggccg agccaggtgc agagctacgt gttgttcgtc ccgggtgaag cccccctcct
     3121 tgtgctccgc ctgtggaacc acgtcccgga tgccgagcga atcgcgatga tcgacgacgt
     3181 ccggtacggc ggggacaccc cgcgtgaaca gggcgagaaa gtcgcgacct tcgcctcgga
     3241 gcacaacgcc cgtcgcatcg ggatgatcgg cgtcgtcccc cacgccgatc tcgccatgat
     3301 ccagcagtcc aacccggaag ccatcctcgt cgacctcaat gaggcctatc aacgattccg
     3361 gctggtgaag agcccggcgg aaatggtgtt cgtgaacatc gcgtcgagga tgaacgatgc
     3421 ggcgatcgac tccctcgctg cgcagctccg tcccggtgtc aacgaatacg aggtcgcgcg
     3481 aatcgtcgag gacgtctacc tggcaaaccg gggctggaac ctcatccact tctccctgag
     3541 cactcccatg gcggcccctg agttctgcgt gccacaccag taccaccccg accggactct
     3601 tcaggcaggt gacgtgttcg tgacggagat aagcacgacg ttctggggct acgcgggaca
     3661 gatcctgcgc actttcacca tcgcctccga cccgacgccc cgctacgagc gactgcacga
     3721 agtggccctg gatgcgtatc agagcatcgt cgaggtcctt cgccccggcg cgaccatcgg
     3781 tgaagtcctc gacgccgccg aggtgataac cgagtcaggt ttcgatatct gggatgacct
     3841 cgtccacgga ttcggcggcg cctatctgcc gccgatcatc cgcactcgtg ccaaccgagg
     3901 cgctactcat cccgaggaat tcgcataccc ggatggcacg ctgctggtgg tgcagccgaa
     3961 cgtcatcgac gggccggccg gagtgcaggc cggcaacagc ctccagatca cgcccgatgg
     4021 cgccgtcagc acgcaacgcc aaccgacgac aatgatcaga tgcgcatgaa gagaaagtct
     4081 tgagcggctc gcctgccgtc gagatgcggg gaatctccaa ggcgttcggc gatacggttg
     4141 ccaacgattc ggttgatttc tccgtccgct ccggggacat acacgtcctc ctcggtgaga
     4201 acggtgccgg gaagaccacc ctcatgaaca tcctcttcgg gcacatcctg cccgacgcgg
     4261 gagtgatcct ggtcgacggc gaggaagtcg agatcagcgc accgaacgat gcgatcgcgc
     4321 acgggatcgg catggtccac caacacttca gcctcgtacc ctcgttcacc gtggccgaga
     4381 acgtgatgat gggcgtgaac cgggtcaccg acctctcctt cagcgacgac gccatcgaac
     4441 aggcagtggc cgatcgagcg gaagagttgc acatgcccat caagcctgac gcgacggtcg
     4501 aggtgct
//
LOCUS       DASTXO010000378         3708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1422918, whole genome shotgun sequence.
ACCESSION   DASTXO010000378 DASTXO010000000
VERSION     DASTXO010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422918"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VFU96_07935"
     CDS             complement(<1..676)
                     /locus_tag="VFU96_07935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5113236.1"
                     /translation="MDIAGKVVVVTGGGNGIGQQVVLELLRRGARVAAVDIREESLNE
                     TVEMAGPGDWLATFVVDITDRTATEALPGQVIAAHGVVDGLINVAGIIQPFVKLNELD
                     YEVIERVLNVNLYGTIHMVKSFLPHLLKRPVAHVANVSSMGGFLPVPGQTIYGASKAA
                     VKLMTEGLYAELLDTNVGVSVIFPGAVATEITSNSGVEIPGNIPNEDEMRARTTSPED
                     AARIILD"
     gene            870..>3708
                     /locus_tag="VFU96_07940"
     CDS             870..>3708
                     /locus_tag="VFU96_07940"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5113237.1"
                     /translation="MQTFLFTDIEGSTRLWEEHGEAMASALARHDDILNAAVGAAGGS
                     VLKTTGDGLIAVFDSVTDGVAAAIDGQRSLEEEPWGQTGPLRVRMGVHSGDTESREGD
                     YFGPAMNRAARIMAAGHGGQVLVSGTAAGAGYLPLGAELLDLGMHRLKDLTEPEHLYQ
                     LVHDALRSEFPAPKTLDGRPNNLPQQATEFLGRKDELKAIHLMLSSPTTRLLTVTGPG
                     GAGKTRISLQVAAEQLDQFPDGVFFVDLSAERDPDSVFDAIAGTLDIPVSGGSDALDV
                     LEARLRDSRLLLVLDNFEQVTRAAVGLSELLQRAPGIKTLVTSRETLRVRAEKVFPVP
                     PMALPHPQDPPSVIADSEAVTLFTDRARAVRPDFAVTEDNAATVADICLRLDGLPLAI
                     ELAAARLNVFTPSDLLGRLRDRLDVLGAGGRDLPDRQRTLWGAIGWSYELLDETEREV
                     FELLSVFSTTDLGALESVATEVLGPINALDSLGSLVDKSLMRSADTGATQRFSMLLMI
                     KEFAMSKLAESPDREEAVRRAHATHFCGLVAGTGGRLHSSERQTALADLEPELGNLRV
                     AWDYWVERDRVEQVFKMIEGMWALHESRGWYRAAIDLANDAIGVLDRADPAPELEAEQ
                     LALRMSLARAMMAVRGYGPETEAAYRRAMEVSETVGTPEQRFPVLRALATYYMGIAQF
                     ATVIEFGRQLLELGEQAGDDSMLIEGHYVFGTATAFSGDLEAGLPHIDRAIELHDPQV
                     HDSGRFRLGPNTGVVARTASGILLWQCGKLEQAVNRVEAALDVARQIGHPYSIAYGLH
                     HNGLLALYRRRFEDAQRWARELAGVSEESDYPVWRTLATVFEGAATSFLGDPETGLSM
                     TEKGIELYHGLTTPPVFWPDLLRLRAQVHAIAGLPERALELIEDALAIGGPDDLTNPE
                     FHIVKGDVLMSFPDPDRAAAEVSYLTAARGAG"
BASE COUNT          636 a         1206 c         1216 g          650 t
ORIGIN      
        1 catcgaggat gatcctggcc gcgtcctcgg gcgacgtggt acgcgctcgc atctcgtcct
       61 cattggggat gttccctggg atctctaccc ccgaatttga tgtgatctcg gtggccacgg
      121 cgccagggaa gatcaccgag acaccgacat tggtgtcgag aagctcggcg tagagcccct
      181 ccgtcatcag cttgactgct gccttggatg ctccgtagat cgtctgacca ggcacaggga
      241 ggaagcctcc catgctcgaa acgttggcga catgggccac gggtcgcttc aggaggtgag
      301 ggaggaacga cttgaccatg tggatggtcc cgtagagatt gacgttgagg acccgctcga
      361 tgacctcgta gtcgagctcg ttgagcttca cgaacggctg gattatgccg gcgacgttga
      421 tcaatccatc gaccacgccg tgggcggcga tcacctgccc ggggagcgct tcggtggcgg
      481 tccgatcggt gatgtcgacc acgaatgtgg caagccaatc accggggccg gccatctcga
      541 cagtctcgtt gagactctcc tcacggatgt cgaccgcggc aaccctggcg ccgcgccgga
      601 gaagctcgag aaccacctgc tgccctatcc cgttcccccc accggtgacg acgacgacct
      661 tgcctgcgat gtccatcccc cgaggctacg cgccggaccg atgagtttga ctcttccgac
      721 agatcggtaa tggagactct cgaggacaaa gggaggcctg atgaccgagc cgacaaccaa
      781 aacgctggag gtgcccggcg cagtgctgac ctacgtgctc gacactggac cctgggtggg
      841 ttagcccccc tttcctatat ttcgggtcga tgcagacatt cctcttcacc gacatcgagg
      901 gaagcacgcg tctttgggag gagcacggtg aggccatggc ctcggccctc gcccgccacg
      961 acgacatcct caacgcggcc gttggcgcag ccgggggctc ggtgctgaag acgactgggg
     1021 atgggctcat tgccgtcttc gactcggtca ccgacggcgt cgccgcggca atcgacggcc
     1081 aacgctccct ggaggaagag ccatgggggc agaccgggcc gcttcgggtg cggatggggg
     1141 tccacagcgg agatactgag agccgcgagg gcgattactt cgggccggcg atgaaccggg
     1201 ccgcccggat aatggcggcg gggcacgggg gtcaggtcct ggtctcgggg accgctgccg
     1261 gcgccggtta tctgcccctt ggcgccgagc tgctcgatct cggcatgcac cgtctcaagg
     1321 acctcaccga gccggagcac ctctatcagt tggtccatga cgcactgcgg tccgagttcc
     1381 cagctcccaa gacactcgac gggcgtccca acaaccttcc tcagcaggcc accgagttcc
     1441 tggggaggaa agacgagctc aaagcgatcc acttgatgct gagctccccc accacgaggc
     1501 tgctcaccgt gaccggcccg ggcggcgccg gcaagacccg catcagtctg caggttgccg
     1561 ccgagcagct cgaccaattt ccagacggtg tcttcttcgt cgatctctcc gccgagcgag
     1621 acccggactc cgtgttcgac gccatcgccg gcactctcga catcccagtg tcgggaggca
     1681 gtgatgctct ggacgtcctc gaggcgcggc ttcgcgactc tcgcctgctg ctcgtcctcg
     1741 acaacttcga gcaggtgacc agggctgccg tcggtctctc cgagctcctc cagcgtgccc
     1801 cgggaatcaa gaccctggtc accagccggg agacactccg cgtacgggcg gagaaggtgt
     1861 tcccggtccc gccaatggca ctgccgcacc cgcaggaccc gccttcggtc atcgccgact
     1921 cggaggcagt gactctgttc accgatcggg cacgggcggt gcgacccgac ttcgcggtga
     1981 ccgaggacaa cgccgccacc gtcgccgata tctgtcttcg tctcgatggg cttccgctcg
     2041 ccatcgagct cgcagcggcc cggctcaatg tgttcacgcc atcggacctg ctcgggcggc
     2101 tccgggatcg gctcgatgtg ctcggagccg gtggccgtga tctccctgat cgtcagcgga
     2161 cgctttgggg ggcgatcgga tggagctacg agctgctcga cgagaccgaa cgtgaggtgt
     2221 tcgaactgct ctcggtgttc tccaccaccg atctcggggc gctcgagtcg gtcgccaccg
     2281 aagtgctggg cccgatcaac gcccttgact cgctcggctc cctggtcgac aagagcctga
     2341 tgcgctccgc ggacacgggg gccacgcagc gcttctcgat gcttctcatg atcaaagagt
     2401 tcgccatgtc gaaactggcc gagtcaccgg atcgggagga ggcggtgcgc cgagcccacg
     2461 ccactcactt ttgcgggctg gtcgcaggaa ccggtggacg gctgcacagc tccgagcgcc
     2521 agaccgcact ggcagacctg gagccggagc tgggcaacct gagggtcgcc tgggattact
     2581 gggtcgagcg ggaccgtgtg gagcaagtgt tcaagatgat cgaggggatg tgggccctgc
     2641 acgagtcgcg gggctggtat cgggcagcta tcgatctggc caatgacgcg atcggcgtcc
     2701 ttgatcgagc cgacccggca ccagagctgg aagccgagca actggcgctt cggatgagcc
     2761 tggcccgagc gatgatggct gtccgtggct acgggccgga gactgaggcg gcgtacagac
     2821 gggccatgga ggtctccgag accgtcggca ctccagagca gcggttcccg gtccttcgtg
     2881 ccttggccac gtactacatg ggcatcgccc aattcgccac ggtcatcgaa ttcggccgtc
     2941 aactgctcga gctcggtgaa caggccggtg acgactcgat gctgatcgaa ggccattacg
     3001 tcttcgggac ggcaaccgct ttcagcggag acctggaggc cgggctgccc cacatcgacc
     3061 gtgccatcga gcttcatgat ccccaggttc acgactcggg ccggtttcgc ctcggcccca
     3121 acaccggggt ggtggcccgg accgcctcgg gcatccttct ctggcaatgc ggaaagctcg
     3181 aacaggcggt gaatcgagtg gaggcagccc tagacgtggc acggcagatc ggccacccct
     3241 acagcatcgc ctacggcctc caccacaacg gtctcctcgc cttgtaccgg aggcgcttcg
     3301 aggatgcaca gcgttgggcg agggagttgg ccggagtctc ggaggagagc gactatccgg
     3361 tatggaggac gctggccacg gtgttcgagg gggcagccac gtcttttctg ggtgaccccg
     3421 agaccggtct cagcatgacc gagaagggaa tcgagctcta ccacggcctg accacaccgc
     3481 cggtgttctg gccggatctg ctccggctgc gcgcccaggt gcacgcaatc gctgggttgc
     3541 cggagcgggc tctggagttg atcgaggacg cgctcgccat cggagggccc gacgacctga
     3601 ccaatcccga gttccacatc gtcaaggggg acgtcctgat gtcgttcccc gaccccgatc
     3661 gcgcagccgc tgaggtgtcg tacctgaccg cggcgcgcgg cgcagggg
//
LOCUS       DASTXO010000379         5007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1054326, whole genome shotgun sequence.
ACCESSION   DASTXO010000379 DASTXO010000000
VERSION     DASTXO010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054326"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VFU96_07945"
     CDS             complement(<1..516)
                     /locus_tag="VFU96_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HEU5113238.1"
                     /translation="MRVVLAYSGGLDTSVILAWLREERGAEVIAYTADVGQGAEAAEA
                     KEKAIATGAEEAVVENLTHRFVEGAVFPALRAGAVYEGHYLLGTSLARPIITEGLVRT
                     ARAFGADAISHGATGKGNDQVRFELSVAALAPDLEVIAPWREWDLDGRTALIEYARER
                     GIPVPVTREEPY"
     gene            complement(519..1931)
                     /locus_tag="VFU96_07950"
     CDS             complement(519..1931)
                     /locus_tag="VFU96_07950"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR02692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEU5113239.1"
                     /translation="MIPKRLEFLVSPDLPPAQLHRLFGAAGHQLFLVGGSVRDAFLER
                     PLEDYDFTTDARPERIAEILEPWADVVYRVGATFGTVAARKDGHTVEVTTFRNEVYRD
                     DSRKPQVTFSDDIETDLARRDFTVNAIALSLMEMEPIDPHGGLPDLAAGVLRTPLEPD
                     ISFSEDPLRMLRLFRFEGTLGFKAEARTVEAVAEMGDRLEIVSAERIRDELSKLVVAP
                     AAGKALGRVVASGLADRFLPELPMLAMEQDPHQRHKDVLAHTLAVVDKTRPDLVLRLA
                     ALFHDIGKPATRQFGKDGVSFHHHEVVGARMTRRRLKALRYSGDVVDAVSRLVFLHLR
                     PHTLKMGWTDSAVRRYVRDAGDLLTDLNELVRCDVTTANPRRARAVQNRIDELEERIS
                     ELSAKEEIARLRAPIDGNQVMEYLGIGPGPEIGDIMGQMLERRIDDGPYSVGEAYAMV
                     RDWAIDRGIPDPGPAPDEEE"
     gene            complement(1990..3069)
                     /locus_tag="VFU96_07955"
     CDS             complement(1990..3069)
                     /locus_tag="VFU96_07955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEU5113240.1"
                     /translation="MEVNIEDLLRRAVSLGATDLHLKVGSPPVARVGGDLERLEGFEA
                     LKPADTQAYAEALFTPGGARDFKDTGTADFAFGRQDIGRFRVTAFRQRGSVSVVLRRV
                     FPGTMSFADLGLPRVVEKLAGESSGLLLVTGASGSGKTSTMASILDWINTNRAVSILT
                     VEDPIEVLHPDKRSVVEQREVGVDTPSAADAVRSAMRHDNDVLMISEFDDAETARAAI
                     AAAETGHLVISSMRTSDPADTIDRIVSLFPEPQQRVIRGQLAAQLLAIISQRLLESHD
                     KGKSLACEVLTNNERVQEWIIGGAEASTLVDIMKESEFFGMQTMDAALLRLVIEKSIE
                     LPAAVPYARNVHELRAKAMAAGIQL"
     gene            complement(3134..4138)
                     /locus_tag="VFU96_07960"
     CDS             complement(3134..4138)
                     /locus_tag="VFU96_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEU5113241.1"
                     /translation="MEVVVAASPMAALHDTGRNHPERPERLGAVLHGLSESGLKVIEI
                     EAPMIERADLALVHDPAYVDAIERFCRLGGGALDMDTYVSSESWGAAVTAVGGVKAVV
                     EELEGRADATGFALCRPPGHHALSSRAMGFCVFNNVAVVAAWLRSQDHRVAILDWDVH
                     HGNGTQALMTDDPGTLYASIHQGGFYPYEGHSDDIEMGAAGTTVNIPLPAGTAGDVFR
                     VAWEKLVIPVVSQFQPDWVLVSAGFDGHTEDPLADLDLLDADYGWMASRLARVHPPHR
                     TVFALEGGYDLDGLRHSARSTVRGMGGEDEFGPPLTSAPGASAAIDVAGIAVARHWSL
                     "
     gene            4156..4881
                     /locus_tag="VFU96_07965"
     CDS             4156..4881
                     /locus_tag="VFU96_07965"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5113242.1"
                     /translation="MPDRLHLDGVWPGPVTIRKGWAKAQARPWNDEFPDPALRLERGS
                     HEFLKAVAQFLGPKGSGMVYSPALYPSATKVWISAGFTLFRRLDVMELPLGGEIRPPD
                     HMIERTGAPDWRALLTIDDLAFDGFWRMTEAGLVEAMRATPRSTVLTALIDDRLAGYA
                     LVGSQMTISFLQRVAVAPSFGGQGIGGSLVRASAVWASKRGARALVLNVRPENDRARR
                     LYEREGFQDRETQLHLLRYQHVA"
BASE COUNT          874 a         1650 c         1621 g          862 t
ORIGIN      
        1 atacggctcc tcacgggtga ccggcacagg gatcccgcgt tcccgggcgt attcgattag
       61 ggcggtgcga ccgtcgaggt cccattcccg ccacggtgcg atgacctcga ggtcgggtgc
      121 cagcgccgcc accgagagct cgaagcgaac ctggtcgttt cctttgcccg tcgcaccatg
      181 ggagatggcg tcagccccga aggcgcgcgc cgttcgcacc aatccctccg tgatgatcgg
      241 ccgagccagc gaggtgccca gcaggtagtg gccctcgtac accgctccgg cgcgaagagc
      301 gggaaacacg gcaccctcga cgaagcgatg ggtcaggttc tcgacgacgg cctcctcggc
      361 accggtggcg atggccttct cctttgcctc ggcggcctcg gcgccctgac caacgtcagc
      421 tgtgtaggcg atcacctccg cgcctctctc ttcgcggagc caggccagga tcaccgaggt
      481 atcgagaccg ccggaatagg cgaggacgac cctcaaggtc attcctcctc gtcgggcgcc
      541 ggcccgggat ccgggatgcc tcgatcgatg gcccagtccc tgaccatggc gtatgcctcc
      601 cccacgctgt aggggccatc gtcgatcctt cgctccagca tctggcccat gatgtcaccg
      661 atctcgggcc ccggcccaat ccccagatac tccatcacct ggttgccgtc gatcggggcg
      721 cgtagccggg cgatctcctc cttggcactc aactcggaga tccgctcctc cagctcgtcg
      781 atgcggttct gcaccgcccg cgcccgccgc gggttggcgg tggtcacatc acacctgacc
      841 agctcgttga gatctgtgag caaatcgccg gcatcgcgca cataacggcg caccgccgaa
      901 tcagtccaac ccatcttgag tgtgtgcggg cgcaggtgga ggaagacgag ccgggagacg
      961 gcatcgacga cgtcaccgga gtaccgcagc gccttgagtc gtcgtctggt cattcgggcc
     1021 cctacgacct cgtgatgatg gaatgagacg ccgtccttgc cgaactggcg ggtggcgggc
     1081 tttccgatgt cgtggaatag ggcagcgaga cggagcacga gatcgggtcg tgtcttgtcc
     1141 acgacggcca gagtgtgggc cagaacgtcc ttgtgccgct gatgagggtc ctgttccatg
     1201 gccaacatcg gcaactcggg caggaagcga tcggccaacc cagaggcgac gacgcgaccg
     1261 agtgccttcc cggcggccgg tgcgacaacg agtttcgaga gctcgtccct gatccgctcc
     1321 gccgacacga tctcgagccg atcccccatc tcggcgacgg cctccaccgt cctggcctcc
     1381 gccttgaacc cgagcgtgcc ctcgaacctg aagaggcgaa gcattcgcag cggatcctcc
     1441 gagaacgaga tgtcgggttc gagcggagtc ctcagcactc cggcggccag atcgggaaga
     1501 ccaccgtgtg ggtcgatcgg ttccatctcc atgagggaga gggcgatggc gttgaccgtg
     1561 aagtcgcggc gggcgagatc ggtctcgatg tcgtcggaaa acgtgacctg gggctttcgg
     1621 gaatcatcgc gatagacctc attgcggaac gtggtgacct ctacggtgtg cccgtccttc
     1681 cgagcggcga ccgttccaaa ggtggcaccg acccggtaaa ccacgtccgc ccagggttcc
     1741 aggatctcgg cgatccgctc cggccgggca tcggtggtga agtcgtagtc ctcgagtggg
     1801 cgctcgagga aggcatcacg caccgaaccg ccaaccagga acagctgatg accggccgcg
     1861 ccgaagagac gatgcaactg ggccggtgga agatccggtg agacgaggaa ttcgagccgc
     1921 ttcgggatca ccggagcgag gttatcgacg cgccgttgac cgcatcggcc gggtcgctgc
     1981 agtcaggacc tacaactgaa tgccggctgc catggctttg gcccgcagct cgtgaacgtt
     2041 tctggcgtag ggaacggcgg ccgggagctc gatcgacttc tcgatcacca ggcggagcag
     2101 tgccgcgtcc atcgtctgca tcccgaagaa ctcgctctcc ttcatgatgt cgacgagtgt
     2161 ggacgcctcg gcgccaccaa tgatccattc ctggacgcgc tcgttgttgg tgaggacctc
     2221 acaagccagc gacttgcctt tgtcgtggct ctcgagcaaa cgctggctga tgatcgcgag
     2281 gagctgcgcc gccagctggc cgcggatcac gcgttgctgc ggctcgggga acagcgacac
     2341 gatgcggtcg atggtgtcgg cgggatcgga cgtccgcatc gaggagatca cgaggtggcc
     2401 ggtctcggcc gcggcgatcg ccgctctggc ggtctcggca tcgtcgaact ccgagatcat
     2461 gagaacgtcg ttgtcgtggc gcatcgcact ccggaccgcg tcggctgcgc tcggggtgtc
     2521 gacgccgacc tcacgttgct ccacgacgct tcgcttgtcc ggatgaagca cctcgatcgg
     2581 gtcctccacc gtgagaatcg aaaccgcccg gttggtgttg atccagtcca ggatcgacgc
     2641 catcgtcgaa gtcttgcccg atccagatgc cccggtgacg aggaggaggc ccgacgactc
     2701 accggccagc ttctcgacga cccgcggcaa accgaggtct gcgaacgaca tcgtcccggg
     2761 gaagacacga cgtagaacga ccgacaccga ccctcgctgt cgaaaggccg ttacccggaa
     2821 acggccgatg tcctgacggc cgaaggcgaa gtcggccgtt ccggtgtcct tgaagtcgcg
     2881 ggcgccgcca ggcgtgaaga gtgcctcggc gtaggcctgg gtgtcggcgg gtttgagagc
     2941 ctcgaatccc tcgagtctct ccagatcacc gccgacgcgg gccaccggtg gggagccgac
     3001 cttgagatgc agatctgtcg cccccaagga gacagcccgg cggagaagat cctcgatgtt
     3061 gacttccatg ttcctttccc agttgaccca gggtgtggtt atcggcgcgg gtcaccgggg
     3121 aactgaagac ttctcagagg ctccaatgcc tcgccacagc aatgccggcg acatcgatgg
     3181 cggcggaggc cccgggcgcc gatgtgagag gagggccgaa ctcgtcctct ccccccatgc
     3241 cacgcaccgt ggagcgagct gaatggcgga gcccatcgag gtcgtacccc ccttcgaggg
     3301 cgaacacggt gcggtggggt gggtggaccc gggccaggcg cgaagccatc cacccgtagt
     3361 cggcatcgag caggtcgaga tcggccagtg ggtcttcggt gtgcccgtcg aacccggcgc
     3421 tcaccagcac ccaatctggc tggaactggg agacgacggg gatcaccagc ttctcccagg
     3481 ccacacggaa cacatccccg gcggtgccgg ccggaagcgg gatgttgacg gtggtgccgg
     3541 ccgcccccat ctcgatgtca tcggaatgac cctcgtaggg gtagaagccc ccttgatgta
     3601 tggatgcata gagagtgccc gggtcgtcgg tcatcagagc ctgggttccg ttcccatggt
     3661 ggacatccca gtccaggatc gccacccgat gatcctgtga cctgagccat gccgccacga
     3721 ctgcgacgtt gttgaacacg caaaacccca tcgcccgtga gctgagggca tgatggccgg
     3781 gagggcggca cagggcgaag ccagtggcat cggcgcggcc ctccaactcc tcgaccaccg
     3841 ccttgactcc gccgactgca gtcacggccg cgccccaact ttcgctcgag acatacgtgt
     3901 ccatgtcgag ggccccgccg ccaaggcgac aaaatctctc gatggcatcg acataggccg
     3961 ggtcgtggac cagggcgaga tcggcgcgtt cgatcatcgg tgcttcgatc tcgatcactt
     4021 tcagcccgct ctcggaaaga ccgtggagca ccgcgcccaa tcgctccggc cgttccgggt
     4081 ggtttcgccc ggtgtcatgc agcgctgcca tcgggctggc ggccaccacc acttccatgc
     4141 cgtactctcc cacaggtgcc cgatcgcctg catctcgacg gcgtctggcc cggcccggtg
     4201 accattcgca agggctgggc caaggcacag gcacggccct ggaacgatga attccccgac
     4261 cccgcccttc gtctggagcg aggctcccac gagtttctga aggcagtcgc gcagttcctc
     4321 ggtccaaaag ggtcgggcat ggtctattcc cctgcgctct atccgtcggc gacgaaggtg
     4381 tggatctccg ccggattcac cctgttccgt cggctcgacg tcatggagct gcccttgggg
     4441 ggagagatcc gcccgccaga tcacatgatc gagcgcaccg gggcccccga ctggagggct
     4501 ctgttgacga tcgacgacct ggcgttcgac gggttctggc ggatgaccga ggccggcctg
     4561 gtcgaggcga tgcgagccac accacgctcc accgtcctca ccgccctgat cgacgaccgg
     4621 ctcgccggtt acgcgctggt gggttcgcag atgacgatct cgttcctgca acgggtcgct
     4681 gttgccccct cgttcggcgg tcagggcatc gggggctcac tagtgcgagc gtcagcggta
     4741 tgggcctcga agcgcggggc gcgggcgctg gtgctcaatg ttcgccccga gaacgatcgc
     4801 gcccggcgtc tctacgaacg tgagggattt caggaccgag agacgcaact tcatctgctg
     4861 cgataccagc atgtcgcatg acggatgaac ccatgaacca ggagccagtc gcccaacgtc
     4921 ggccggccat agaagatgcc accgccggtc tctgatcgct accgcctcga gatgcgactc
     4981 gggcgagaca acgacatcga ggaatgg
//
LOCUS       DASTXO010000380         4262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_686625, whole genome shotgun sequence.
ACCESSION   DASTXO010000380 DASTXO010000000
VERSION     DASTXO010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686625"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="VFU96_07970"
     CDS             complement(<1..1091)
                     /locus_tag="VFU96_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016352126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5113243.1"
                     /translation="MRSRILLLTMVMAVIAAACTSSGGEEDGAELPTEVGDGEGSVVI
                     VSWAGYIERGDTDPAFDWVTEFEEETGCAVENKIAGTSDEMVALMTGSDEYDLVTASG
                     DSSLRLISGGSVQEINIDLIPSWDTVDERLQDAEWHTVDGAHYGVPYSSGFNVLGYNT
                     EVFGDTPPDSWSVVFEETTLPDGESNAGRVQAYDGPIYVADAALYLSATQPDLGIEDP
                     YQLNTEQYQAALELLRQQRELVSRYWHDAFVQIDDFTNEGVAASSTWPFQTNVLQAGG
                     APIDKVVPVEGATGWADTTMMSSNAPHPNCAYKWMEWSLNPKLQGDLAAWFGTVPAVP
                     AACDGNELLGPDGCANNGSESFDEILFWKT"
     gene            complement(1088..2194)
                     /locus_tag="VFU96_07975"
     CDS             complement(1088..2194)
                     /locus_tag="VFU96_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004559642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5113244.1"
                     /translation="MPPAVTLDDVAKSFGDVVAVDGVTLDIADGEFFSMLGPSGSGKT
                     TCLRMIAGFEIPTTGRVLLHGKDMTGLPPYDRDVNTVFQDYALFPHMTVAQNVGYGPM
                     VQKMSEAERSDRVSSALAMVHLDAFGDRKPSQLSGGQRQRVALARALINRPAVLLLDE
                     PLGALDLKLRQAMQLELKTLQREVGITFIYVTHDQEEALTMSDRLAVFNHGKVEQVGT
                     PSEVYENPRNSFVAGFVGTSNVMSGPLAERILGSPAPFTVRPEKITIESPSGEPRTDG
                     LAVNGTVVEAVYLGMYTRYRVDVGDGVIEVAAQNTGISHSEAQQQVGRQVRLTWAKSS
                     CRPLEESPRDGEPDEKAPVLTGAGNNTSEEETEV"
     gene            complement(2247..3788)
                     /gene="eat"
                     /locus_tag="VFU96_07980"
     CDS             complement(2247..3788)
                     /gene="eat"
                     /locus_tag="VFU96_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine permease"
                     /protein_id="HEU5113245.1"
                     /translation="MSTERKVGSVTYRDVDQGYFDQRGLKRHAGVWMLWALGVAAVIS
                     GDFSGWNFGLDVGGFGGLFIATIVITIMYIGLCYSISEMSPALPHTGGAYSFARSSMG
                     PWGGFITGVAENIEYVVTPVVVVYFASIYAQSVFNDLFGFELGLPWWWVIFYAIFVGL
                     NIIGIEATMRFSVVIAVISLAVLAVFFVLAIPNFDASLLTNIVAEEGGTSFLPFGIAG
                     IFAALPFAIWFYLAIEELPLAAEESMDPKRDVPRGTMWGLWTLVLTGMGVLFLNTGIG
                     GGAEGIRTSGEPLLDGFRQVLGSDTSAAILGLFALTGLIASFHTIIYAYGRNIYSLSR
                     AGYFPHWMSVTHGKRKTPYVALLLGAVVGLALAFLLDSQYSGTAGAALLSMAVFGAVI
                     SYFMQMLSFIRLRRNMPNIQRPYRSPVGVFGAALAGLIALVSLVALFLNDAYRPGVYG
                     VAIWFVLALLYFALSGRNKLVLSPEEEFAMTQGEHGHPEEEGYGTTSVADTTATDTTV
                     PDPQA"
     gene            3947..>4262
                     /locus_tag="VFU96_07985"
     CDS             3947..>4262
                     /locus_tag="VFU96_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEU5113246.1"
                     /translation="MGELRGLISIDELEALVASDEVDTVVTAFTDHYGRVHGKRFTAR
                     HFLDEIFDHGTHGCDYLLTVDMEMEPVPGYTYASWDLGYGDFHMVPDLSTLRVATWLD
                     RTA"
BASE COUNT          800 a         1360 c         1328 g          774 t
ORIGIN      
        1 gtcttccaga acaggatctc gtcgaaggac tcgctcccgt tgttggcgca tccatccggg
       61 ccgagcaact cgttgccgtc acaggcggcc gggaccgcgg ggacggtgcc gaaccaggca
      121 gccaggtcac cctggagctt ggggttcaac gaccactcca tccacttgta ggcgcagttc
      181 gggtgtggag cgttggagct catcatcgtg gtgtcggccc atccggttgc cccctccacc
      241 gggaccacct tgtcgatggg cgcgccgcct gcctgaagga cattggtctg gaacggccag
      301 gtcgatgagg cggccacgcc ttcgttggtg aagtcgtcga tctggacgaa ggcgtcgtgc
      361 cagtagcgac tcaccaactc gcgttgttgc ctgagcagtt cgagagccgc ctggtattgc
      421 tccgtattga gctggtacgg gtcctcgata cccaggtcgg gttgcgtggc gctcaggtac
      481 agggcggcgt cggcgacgta gatcgggccg tcgtaggcct gaacacgccc ggcgttggac
      541 tcaccatcgg gcaaagtcgt ctcttcgaag acgacgctcc acgagtccgg tggcgtgtcg
      601 ccgaacacct cggtgttgta gccgagcaca ttgaaaccgg acgaataggg cactccgtag
      661 tgcgccccgt ccactgtgtg ccattccgcg tcctgaagtc gctcgtcgac cgtgtcccaa
      721 ctcgggatga ggtcgatgtt gatctcctgg acggacccac ctgaaatcag cctgaggctg
      781 gagtcgcccg aggctgtgac caggtcgtac tcgtccgatc cggtcatcaa agccaccatc
      841 tcatctgacg tgcccgcgat cttgttctcg accgcacacc cggtctcttc ctcgaactcg
      901 gtcacccagt cgaaagccgg gtcggtatcg ccccgctcga tgtatccggc ccaggagacg
      961 atgaccacgc ttccttcgcc atcgcccacc tcggtgggca gctctgcgcc gtcttcctcc
     1021 cctcctgagc tggtacaggc ggccgcgatg accgccatga ccatggtcaa gagaagtatt
     1081 cgtgatctca tacttcggtt tcctcctctg aggtgttgtt cccggctccg gtgagcaccg
     1141 gagccttttc atccggctcc ccatctcgag gggattcctc gagtggtcgg cacgaactct
     1201 tcgcccaggt gagcctgacc tggcgaccca cttgctgttg ggcttcgctg tggctgattc
     1261 ctgtgttctg ggcggcaacc tcgatgacgc catcgccaac gtcgacccgg tatcgggtgt
     1321 acatgcccag gtagaccgct tcgaccacgg tcccgttcac tgcgaggccg tcggtccgtg
     1381 gttcacccga cggtgattcg atcgtgatct tctcggggcg gacggtgaac ggtgccggcg
     1441 atccaagaat gcgctctgcc agcggcccgc tcatcacgtt ggaggtgccc acgaatccgg
     1501 cgacgaagga gtttcggggg ttctcgtaca cctcggacgg cgtgccgacc tgctcgacct
     1561 tgccatggtt gaacacggcg agtcggtcac tcatggtgag ggcctcctcc tggtcgtggg
     1621 tgacgtagat gaaggtgatc ccgacctcgc gctgcagggt cttcaactcc aattgcatgg
     1681 cctgacgcag cttgaggtcg agggcgccca ggggctcatc gaggaggagc acggccgggc
     1741 ggttgatcaa ggctcgcgcc agcgcaactc tctgtcgctg cccgccggat agctgactcg
     1801 gcttgcgatc cccaaaggcg tcgagatgga ccatcgccag cgcgctcgag acgcgatcgg
     1861 accgctccgc ctcgctcatc ttttgcacca ttggcccgta gcccacgttc tgcgccacgg
     1921 tcatgtgggg gaagagggcg tagtcctgga agacggtgtt gacgtcccgg tcataggggg
     1981 gcagcccggt catgtccttt ccgtggagca gcactctccc cgtggtcggg atctcgaagc
     2041 ccgcgatcat ccgcagacaa gtggtcttcc ccgaacccga agggccgagc atcgagaaga
     2101 actcgccgtc cgcgatgtcc agagtcaccc cgtcgaccgc gacgacgtcg ccaaacgact
     2161 tggcgacatc gtccagggtc accgcgggcg gcatcagcgg ggcccccggt cgtcgggccg
     2221 gtggccgatc ggggagggca gctgtctcag gcctgagggt ctggcaccgt cgtgtcggtc
     2281 gcagtcgtgt ccgctaccga cgtcgtgccg tatccctcct cttcgggatg accgtgctcg
     2341 ccctgagtca tggcgaactc ctcttcgggt gatagaacca gcttgttccg cccggagagg
     2401 gcgaaataga gcagggcgag gacgaaccag atggcaaccc cgtacacccc tgggcgatag
     2461 gcgtcgttca agaacaacgc caccaacgac accagggcga tcaggccggc caaggccgcc
     2521 ccgaagaccc ccaccgggct ccgatacggt cgttggatgt tcggcatgtt ccgcctgagc
     2581 cggatgaagg acagcatctg catgaagtag gagatcaccg ctccgaacac cgccatgctc
     2641 agcagagcgg ctcctgcggt cccgctgtac tgactgtcca gcaagaaagc cagtgcgagc
     2701 ccgacgaccg ctccgaggag aagcgccaca tacggtgtct tccgcttccc gtgagtcacc
     2761 gacatccagt gggggaagta gcccgccctc gacagcgagt agatgttccg cccgtaggca
     2821 tagatgatcg tgtggaagct ggcgatcagc ccggtgagag cgaagagccc aaggatggcg
     2881 gcactggtgt cactgcccaa gacctgccgg aagccgtcca gcaagggctc acccgacgtc
     2941 ctgatcccct cggcgccacc accgatcccg gtgttgagga agagaacccc cattccggtg
     3001 aggaccaggg tccacagacc ccacatcgtc cctctcggaa cgtcgcgctt ggggtccatc
     3061 gattcctcgg cggcgagggg cagttcctcg atcgccaggt agaaccagat cgcgaagggc
     3121 aaggcagcga atatcccggc gatgccaaat gggaggaacg atgtgccgcc ctcctcggcg
     3181 acgatgttgg tcagcaacga agcatcgaaa ttggggatcg ccagcacgaa gaaaacggcg
     3241 agaaccgcga gcgatatcac ggcgatcacc accgagaacc gcatcgttgc ctcgatcccg
     3301 atgatgttga gcccgacgaa gatcgcatag aagatcaccc accaccaggg caggccgagc
     3361 tcgaagccga acaggtcgtt gaacacgctc tgggcataga tcgaggcgaa atagaccacc
     3421 acgacgggtg tcaccacgta ttcgatgttc tcggccacac cggtgatgaa tccgccccac
     3481 ggtcccatcg acgaccgggc gaacgaatag gcccctccgg tgtggggtag ggcaggcgac
     3541 atctccgaga tggagtagca gagcccgatg tacatgatcg tgatgacgat cgtggcgatg
     3601 aacagaccgc cgaatccgcc gacgtctagg ccgaagttcc atccagagaa gtcacccgag
     3661 atgactgcgg ctaccccgag cgcccagagc atccacacgc cagcgtgacg cttcaagccg
     3721 cgctgatcga agtacccctg gtcgacatcg cgataggtga ccgagccgac cttgcgttcg
     3781 gtcgacatag acgtacctcc cttggtgagt gggcatggag aataggaatg cacgtgtccc
     3841 cgatgccgtc cgcatcagta gttttttgat tcgccattca gcgacagcag gtggctaccg
     3901 cggcctaccc tccctgcacc gagaccgagg gcaaggagcg cgccgaatgg gcgaattgcg
     3961 gggattgatc agcatcgacg agctggaggc gctggtcgcc tccgacgagg tcgacacggt
     4021 ggtgaccgcg tttaccgacc actacgggag ggttcacggg aaacgcttca ccgcacgtca
     4081 ctttctcgac gagatcttcg atcacgggac ccacggttgc gactacttgc tgacagtcga
     4141 catggagatg gagccggtgc cgggctacac ctacgccagc tgggatctcg ggtacgggga
     4201 cttccacatg gtgcccgacc tgagcacgct tcgtgtcgcc acctggctgg atcgaacggc
     4261 ct
//
LOCUS       DASTXO010000381         5521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1202176, whole genome shotgun sequence.
ACCESSION   DASTXO010000381 DASTXO010000000
VERSION     DASTXO010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5521
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202176"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VFU96_07990"
     CDS             complement(<1..321)
                     /locus_tag="VFU96_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEU5113247.1"
                     /translation="MSGDLARALLTGADFEVIPLKGVMERALSIEPGSTVSVTASPTR
                     GMTPTVDLASELQARGYRAVPHRSARTIENRGDLSSSIKRLDHGGVGQVFITGGDGEP
                     SGAYR"
     gene            complement(324..1862)
                     /locus_tag="VFU96_07995"
     CDS             complement(324..1862)
                     /locus_tag="VFU96_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEU5113248.1"
                     /translation="MEQTSTGRRGGGRAARQAARSARAIESVPFLTRKLAPVEVLDEE
                     GLSLIEANADMLLEKVGIEVGNFPEALDIFRDGGCEVSETRVRFPTGLARSLLATAPS
                     KFTQHARNPDHDVVIGDPHLVFAPAYGSPFVRDLEEGRRYATLADFENFARLTHVTPA
                     LHHGGGTLCEPVDLPVNKRHFDMVLAHIKCHDRAFMGSVTAPERAQDTVDMVRIVFGD
                     EFDQGKTVLLSLCNANSPMSWDYNMLGSAKVYAENNQACLITPFILSGAMAPVTVAGT
                     ATQTLAEAMAGMAFCQLVRPGAPVVFGSFASSISMQSGAPTFGTPEPAMVLFVMAALA
                     RRLGVPFRSGGNLTAAKTPDYQAAFESAATFLPTLLAGVNFVLHTAGWLEGGLVMGYE
                     KFVLDADQASMAEALIGGVDLSDNGLALEALLDNPPGQHFLGNSHTLANFENAFWRSA
                     TADNNSFEQWLEAGSQEAATRANGIWRRMLAEYEPPPLDESIDAELAEWVAQRKASFP
                     DSDV"
     gene            complement(1914..3866)
                     /locus_tag="VFU96_08000"
     CDS             complement(1914..3866)
                     /locus_tag="VFU96_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018240079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HEU5113249.1"
                     /translation="MTRQVVFSPSGRRGAFPEGTTVLDAARALGVDLDSVCGGRGICG
                     RCQVVIGSRPGVEARADRLSELGPTETDYQGRRPLGSGHRLGCAARIVDDVVIDVPPD
                     SQVHRQVVRKRAGVAALPVDPVVRLHYIEVPEPDLASPTGDLERLLGALEQEWGLTGL
                     VVDSAVLRILQATLRQDGWAVTVAVRQGRDLIGIWPGFRDLALGVAFDVGSTTIAGHL
                     CDLLTGAVLATNGAMNPQIGFGEDLMSRVSYVMMNDDGAQRLTRVVRDALDELLGELV
                     TEAGVDRGDVLELSIVGNPIMHHLALGLDPRELGTAPFALATTSALHIPASELGLRAH
                     DGARVYSLPCIAGHVGADAAGAVLSEGPHRAERLQLLVDVGTNAELVLGNKDRLLAAS
                     SPTGPAFEGAQISSGQRAAPGAIERVRIDPSTLEPRFSVIGQEVWSDEPGFDSGAVTG
                     ICGSGIIEVIAELHLAGVIDSDGVIRPPVEPSPRVVASDRTFSYVLCVEPLIEVTQND
                     VRAVQLAKAALHAGARLLMDHYGVEKVDEIRLAGAFGSHIDPLHAMVLGMIPDCDISR
                     VSAAGNAAGVGAMMALLSGVARTEIEEVALMIEKVETAVEPRFQEHFVAALAIPHKSD
                     PYPLLSKVVDLPPRPDPGERRRRRPG"
     gene            complement(3873..4769)
                     /locus_tag="VFU96_08005"
     CDS             complement(3873..4769)
                     /locus_tag="VFU96_08005"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007232234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU5113250.1"
                     /translation="MTDTVVSSETREVVIGFERPFVLIGERINPTGRRLLAEEMKAGD
                     FSRVETDAISQVGAGAQMLDVNAGIPLADEPALLAQAIELVQSLTEVPLSIDSSIVAA
                     LESGLLAYRGKALVNSVTGEEERLEQVLPLVAKHGAAVVAISNDETGISEDPDVRFEV
                     AKKIVGRAADHGIPREDVVVDPLVMPIGAMATAGRQVFHLIRRLREELGVNTTCGASN
                     ISFGLPNRAGINAAFLAMAIGAGMTSAITNPLEPEIRQAILAADVLGGRDPNCALWIK
                     TFREPPAAGEEGRRRENRRRRG"
     gene            complement(4766..5092)
                     /locus_tag="VFU96_08010"
     CDS             complement(4766..5092)
                     /locus_tag="VFU96_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HEU5113251.1"
                     /translation="MSPKEARHRDRGPQLTVIRWREIPAQVLARRGDETAKRELSARF
                     QVAIDRAAMYAGLFGTDEYLAEWARDTRPCGDDLETEVTAEAARLEAEYDSDVLNELA
                     ANGGAR"
     gene            complement(5089..>5521)
                     /locus_tag="VFU96_08015"
     CDS             complement(5089..>5521)
                     /locus_tag="VFU96_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HEU5113252.1"
                     /translation="GRYRSVFVGYADCGTGGLLDRMLERRGVGRLPGAHCYELYSSPS
                     GFMALHDREPGTFYLTDFLTRHFDRLVWVGLGLDRWPGLRDTYFGNYRRLVYLSQVPT
                     PKLVAGATAAARRLGLTFEHRHTGYGDLASTLVEVTRRVPA"
BASE COUNT          987 a         1840 c         1787 g          907 t
ORIGIN      
        1 tctataggcc ccgcttggct cgccgtcgcc gcctgtgatg aagacctgtc ctactcctcc
       61 atggtcgagc cgcttgatgc tcgaggagag atcgccgcgg ttctcgatgg tcctggccga
      121 ccgatgaggc accgcccgat agccccgagc ctggagctcg gacgccagat ccaccgtggg
      181 ggtcattcca cgagtcggcg aggcggtgac cgagaccgtc gatcccggct caatcgataa
      241 tgccctctcc atcacgcctt tcaacgggat cacctcgaaa tcggccccgg tcagaagcgc
      301 ccgagcgaga tcgccgctca tgctcaaacg tccgagtcgg ggaaggaggc tttgcgctgg
      361 gcgacccact cggccaattc cgcgtcgatc gactcgtcca gagggggcgg ctcgtactcg
      421 gccagcatcc ggcgccagat gccattcgca cgtgtggctg cctcctggga tcctgcctcg
      481 agccactgct cgaagctgtt gttgtccgcc gtggcactac gccagaaagc attctcgaaa
      541 ttggccaggg tgtgtgagtt gcccaggaag tgctggccag gcgggttgtc gaggagggcc
      601 tcgagggcca gtccgttgtc agagaggtcg acacccccga tcagagcctc cgccatcgag
      661 gcctgatccg cgtcgagaac gaacttctcg tatcccatca ccaatccccc ctccagccac
      721 ccggcggtat ggagtacgaa gttgaccccg gcaaggagag tggggagaaa ggtggcggcc
      781 gactcgaatg ccgcctggta gtcaggggtc ttcgccgccg tgaggttccc cccgctccgg
      841 aaggggacgc cgagccgccg ggccagcgcg gccatcacga acagaaccat tgccggctcg
      901 ggggtgccga atgtcggcgc cccggattgc atcgagatcg acgacgcgaa cgatccgaac
      961 acgaccggcg ccccgggtcg caccaactgg cagaacgcca taccggccat cgcctcggcc
     1021 agcgtctggg tcgcggtccc ggccacggtg accggggcca tcgctccgga aaggatgaac
     1081 ggcgtgatga gacacgcctg gttgttctcg gcgtagacct tcgccgagcc cagcatgttg
     1141 tagtcccagc tcatcgggct gttggcattg cacagggaga gcaggaccgt cttgccctgg
     1201 tcgaactcgt cgccgaagac gatcctgacc atgtccacgg tgtcctgggc tcgctcgggg
     1261 gcggtgaccg agcccatgaa cgctctgtcg tggcacttga tgtgcgccag aaccatgtcg
     1321 aaatgccgct tgttcaccgg caggtcgacc ggctcgcaca gggtgccgcc gccgtgatgg
     1381 agcgccggcg tcacgtgggt caaccgcgcg aaattctcga agtcggcaag ggtcgcgtag
     1441 cggcgaccct cctcgaggtc ccgtacgaag ggtgaaccat aggcgggggc gaaaacgagg
     1501 tgggggtcac cgatcacgac gtcgtggtcg ggattacgcg cgtgctgggt gaacttcgat
     1561 ggcgctgtcg ccaggagact cctggccagg ccggtcggga atctgacccg ggtctcggag
     1621 acctcacaac ccccgtcccg gaatatgtcg agagcttcgg gaaagttgcc cacctcgatg
     1681 ccgactttct cgaggagcat gtcggcgttg gcctcgatga gggacagacc ttcctcgtcg
     1741 agaacctcga ctggcgcgag tttgcgcgtc aggaatggca ccgattcgat ggcccgggcg
     1801 gatcgtgccg cctgacgggc ggcgcggcca ccgccccgac gtcccgtgct ggtctgttcc
     1861 atgatgcggt accagactac atcatggaac aagcaccgga aagggcccga gcatcatccg
     1921 ggccgtcgtc gtcgcctctc gcccggatcg ggtcggggcg gcagatccac gactttggac
     1981 aagagtgggt agggatcgct cttgtggggt atggccagag cggccacgaa gtgctcctgg
     2041 aatcgtggct cgaccgcagt ctccaccttc tcgatcatca gggccacctc ctcgatctcg
     2101 gtgcgggcga cgccggagag aagggccatc atcgccccga ccccggcggc attcccggcc
     2161 gccgagactc gtgagatatc gcagtcgggg atcatcccca gcaccatggc gtgcagggga
     2221 tcgatgtggc tgccgaaggc tccggccagc ctgatctcgt cgaccttttc cactccgtag
     2281 tggtccatga gaagacgtgc cccggcatga agcgcagcct tggccaattg gacggccctc
     2341 acgtcgttct gggtcacttc gatcaacggc tcgacgcaca ggacatagga aaaggtgcga
     2401 tctgaggcaa ccacccgagg cgaaggttcg accggcggtc ggatcacgcc atcggaatcg
     2461 atcacgccgg caagatggag ctcggcgatg acctcgatga tcccggatcc acagatgccg
     2521 gtcacggcac ctgagtcgaa accgggctcg tccgaccaca cctcctgtcc gatgacggag
     2581 aagcggggct cgagggtcga agggtcgatt ctcacccgtt cgattgcccc cggcgcggca
     2641 cgctgcccgg acgagatctg cgctccctcg aacgccggcc cggtcgggct cgaagcggcg
     2701 agcagccggt ccttgtttcc cagcaccagc tcggcattgg tcccaacgtc gacgagcagc
     2761 tgcagtcgct cggcacgatg tgggccttcc gacaacaccg caccggccgc gtcagcgccc
     2821 acatgacccg cgatgcaggg cagcgagtag accctggcgc catcatgggc acgcagcccg
     2881 agctccgagg ccgggatatg gagggccgag gtggtggcaa gggcaaaggg agcggtgccc
     2941 agttcccgag gatcgagccc gagagccaga tgatgcatga tcgggttgcc gacgatgctc
     3001 agctcgagga cgtcaccgcg gtccacccct gcctccgtga ccaactcccc gagcagctca
     3061 tctagggcgt ctcgaactac tcgcgtgagg cgttgcgccc cgtcgtcgtt catcattacg
     3121 taggagaccc ggctcatgag gtcctcgcca aacccgatct ggggattcat cgccccattg
     3181 gtcgccagga cggcgcccgt cagcaggtca cagaggtggc cggcgatggt ggtggagccg
     3241 acatcgaagg cgacaccgag ggcgagatcg cgaaaaccgg gccagatccc gatcagatct
     3301 ctcccctgcc gcacggccac cgtcaccgcc caaccatcct gacgcagggt ggcctggagg
     3361 atcctcagca ccgcggaatc gacgacgagc ccggtcagcc cccactcttg ttcgagggcg
     3421 cccaggagac gctcgagatc accggtgggg ctggccagat cgggctcggg gacctcgatg
     3481 tagtgcagcc ttacgacggg gtcgaccggc agggcagcta cacctgcgcg ctttcgcacc
     3541 acctgacgat gaacctggct gtcgggcgga acatctatga ccacgtcatc cacgatccgg
     3601 gcggcacagc ccagccgatg tccggatccc aacggtcgac gaccctgata atcggtctcg
     3661 gtcggtccca actcggagag ccgatccgct cgggcctcga cgccaggtcg gctaccgatc
     3721 accacctggc agcggccgca gatgcctctt cccccacata ccgaatcgag gtccacgcca
     3781 agcgctcgag ccgcgtcgag aaccgtggtg ccctccggaa acgcaccgcg tctcccggaa
     3841 gggctgaaca ccacctgacg ggtcacggcc agtcagccgc gtcggcggcg gttctcacga
     3901 cgacgacctt cctcgccggc ggcgggtggc tctcgaaagg tcttgatcca gagcgcgcaa
     3961 ttggggtcgc ggccaccaag gacgtcggcc gcaaggatcg cctgacggat ctcgggctcg
     4021 agagggttgg ttatggccga ggtcatgccg gccccaatcg ccatcgccag gaaggcggcg
     4081 ttgatcccgg cgcggttggg taggccgaag ctgatgttcg aagctccgca ggtggtattc
     4141 acccccaact cctcgcgcag ccggcgaatg aggtgaaaca cctgtcttcc ggcggtggcc
     4201 atggcgccga tcggcatcac cagcggatcg acgacgacat cttctcgggg tatgccgtga
     4261 tccgcggccc ggccgacgat tttcttcgct acttcgaacc ggacgtccgg gtcctccgag
     4321 attccggtct cgtcgttgga tatcgccacc acggccgccc catgtttcgc gacgagcggc
     4381 aacacctgct ccagacgctc ctcctccccg gtgaccgagt tcaccaacgc cttgccccgg
     4441 tatgccaaga ggccagactc gagcgcggcg acgatcgacg agtcgatgga cagaggaacc
     4501 tcggtcaatg actggaccag ctctatggct tgggcgagaa gggcaggctc gtcggcgagg
     4561 gggatgccgg cattgacgtc gagcatctgc gcgccggccc cgacctggct gatcgcgtcc
     4621 gtctccaccc gggagaaatc gccggccttc atctcttcgg cgagcaggcg ccggccggtc
     4681 gggttgatcc tctctccgat cagcacgaaa ggacgctcga acccgatgac cacctcacgg
     4741 gtctccgagg agacgacggt gtcggtcatc gcgccccccc gtttgcggcg agctcgttga
     4801 gcacgtcgga gtcgtattcg gcctcgagac gcgccgcttc ggccgtgact tcggtttcga
     4861 gatcatcccc acagggcctg gtgtcccggg cccactccgc cagatactcg tcggtcccga
     4921 acaagccggc gtacatggca gcccgatcga tggcgacctg gaaccgggcc gagagctcgc
     4981 gtttcgcggt ctcgtctccc cgccgtgcca acacctgggc ggggatctcc cgccagcgaa
     5041 tgacggtcag ctggggacca cgatcgcggt gcctggcttc tttggggctc acgccggcac
     5101 ccgtcgcgtg acctcgacga gggtggaggc cagatcgccg tatccggtgt gccggtgctc
     5161 gaaggtgaga ccgagtcggc gggccgccgc cgtcgcgccg gcgaccagtt tgggggtagg
     5221 aacctgcgag aggtagacca gccgtcgata gttgccgaag taggtgtcac gaagacccgg
     5281 ccatcgatcg agccccagac ccacccagac cagacgatcg aagtgtcggg tcaagaaatc
     5341 ggtcagatag aacgtgccgg gctctcggtc gtgaagggcc atgaatcccg agggagacga
     5401 atacaactcg tagcaatgcg ccccggggag ccgacccacc ccccttcgtt cgagcatccg
     5461 gtcgagaagt ccgccggtcc cgcagtcggc ataacccacg aagacgctcc ggtatcgccc
     5521 t
//
LOCUS       DASTXO010000382         3242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1017267, whole genome shotgun sequence.
ACCESSION   DASTXO010000382 DASTXO010000000
VERSION     DASTXO010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017267"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VFU96_08020"
     CDS             <1..550
                     /locus_tag="VFU96_08020"
                     /inference="COORDINATES: protein motif:HMM:NF014486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HEU5113253.1"
                     /translation="VPSVRGKPPVLVGEEDNPIAVLVGAVGLFVALVLVGVVGPALPT
                     ENPGARTSDLPYTETALHGQEIYQSVGCASCHTQMVRPVVADVGLGAVSLNDTNQVLA
                     SRRFGPDLSNVGTRITAGQIEAIILGLDDHPSLSLSTDDLDALVAYLVESATLEEAPP
                     PPPGDEEPAPEGETPTEETTAS"
     gene            547..1590
                     /locus_tag="VFU96_08025"
     CDS             547..1590
                     /locus_tag="VFU96_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU5113254.1"
                     /translation="MSDLSAAAAALGIPEAIVQRSAAARAAETGMTVDEVLAAWAGGG
                     SVSGAPAEASEPAPAAEQTATASETAGEPEEETVAEEVPAGLAAAPPSAPSGPVTRSP
                     IPAEVTAAEAAGLPEVITVPTAGIRERTNLAIPRWLAAVMLIIPAFALFALGGSATGE
                     CGEDTELATNVITGQVVNCDGTEFTGSGAGGGGGDFVAMGEDIYLNVASCDGCHGAQG
                     QGGVGPGFSGVLTTFGSCADHIEWVTLGSSGFQEAGEATYGDTGKPIAGGMPSFGGTL
                     TEEQLASVVAFERVRFGGADPAETLTDCGLAPAEGEEGGAPGETTPDGESPADGETTT
                     TVSGAEAAAARVP"
     gene            complement(1613..1867)
                     /locus_tag="VFU96_08030"
     CDS             complement(1613..1867)
                     /locus_tag="VFU96_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEU5113255.1"
                     /translation="MNNSAFRFLIRPGCHLCDEARPVVEKAVARVGAALNEIDIDADD
                     ALTRDYGLRVPVLLGPGDVVLAEGRIEAGALRRALRRSRT"
     gene            complement(2144..2977)
                     /locus_tag="VFU96_08035"
     CDS             complement(2144..2977)
                     /locus_tag="VFU96_08035"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEU5113256.1"
                     /translation="MKHNALPISFLSDFGHADEFVGVVHGVLAKLAPESRVIDVGHGS
                     ARGDVRGAALSLTRAIQYLPEGVALVVVDPGVGSDRLALALETDWGFFVGPDNGVLSP
                     AVAMVGGARVMVSIDNPEARIPSPGETFHGRDVFAPAAALLASGEAAIQDLGPEVGPD
                     AVQSLLLPLPETQGSLVTGEAWWVDRFGNVQTNIGPEDLAAVGLSPGSVLTMKVGATL
                     HTILWAVAYSEVEPGEPLVHVDSAGMIAIAVRDGAADAELNLSAGVAVTLAGTGSTST
                     G"
     gene            2999..>3242
                     /locus_tag="VFU96_08040"
     CDS             2999..>3242
                     /locus_tag="VFU96_08040"
                     /inference="COORDINATES: protein motif:HMM:NF027889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp1/GlmU family protein"
                     /protein_id="HEU5113257.1"
                     /translation="MSETSHDGVAREVSRARVAMLMGSVDTGKTTLALQAARLALGAG
                     RTVAIVDADVGNSTIGPPACVGLKVLRSPDDLDDVNT"
BASE COUNT          526 a         1145 c         1059 g          512 t
ORIGIN      
        1 ggttccttcc gtccggggca agccaccggt gctggtcggc gaggaggaca acccgatcgc
       61 ggtgctggtc ggggcggtgg ggctgttcgt tgccctcgtc ctggtcggtg tggtcggccc
      121 ggcattgccc acggagaacc cgggggcacg taccagtgac ctgccctaca cggagacggc
      181 cctgcacggg caggagatct accaatccgt tggctgcgcc tcttgccaca cgcagatggt
      241 tcgcccggtt gtggcagacg tcggtctcgg agccgtgtcc ctgaacgaca ccaatcaggt
      301 cctggcctcg cggcgattcg gcccggatct ttccaacgtg ggaacgcgga tcacggccgg
      361 tcagatcgag gcgatcatcc tcgggctcga cgaccatccc tcgctttcgc tctcaacgga
      421 tgatctcgat gccctggtgg catacctggt cgaatcggcg accctcgagg aagccccgcc
      481 tccccctccc ggtgatgagg aaccggctcc cgaaggggag acacccaccg aggaaaccac
      541 ggcctcatga gcgatctgag cgccgccgct gccgctctcg gcatccccga ggccatcgtc
      601 cagcgctccg ccgctgcccg tgccgccgag acggggatga cggtcgacga ggtcctggcc
      661 gcatgggcgg ggggtgggtc ggtgtccggt gccccggcag aggcatccga gccggcaccc
      721 gctgccgagc agacggcgac tgcctccgag actgctggcg agcctgagga ggagaccgtt
      781 gctgaggagg tccccgccgg gctggccgcg gcccctccca gtgcgccctc cggcccggtg
      841 acccgctccc cgatcccggc cgaggtgact gcggccgagg ctgccgggct tcccgaagtg
      901 atcacagttc ccaccgcggg catccgggag aggacgaacc tggccatccc ccgctggctg
      961 gccgccgtga tgctgatcat ccccgccttc gcactcttcg cactcggcgg ctccgccacc
     1021 ggcgagtgcg gtgaggacac cgagctcgcc accaacgtca tcaccggcca ggtggtcaat
     1081 tgcgacggca ccgagttcac cggatctggt gccggtggcg ggggcggcga cttcgtcgcc
     1141 atgggcgagg acatctacct gaatgtggcc agctgcgacg ggtgccatgg cgcccagggg
     1201 cagggcggcg tcggcccggg cttcagtggt gtcctcacca cgtttgggtc gtgtgccgat
     1261 cacatcgaat gggtcaccct cggctcgagc ggcttccagg aagccgggga ggccacttat
     1321 ggcgacaccg gaaagcccat cgccggtggc atgccctcgt ttggcgggac tctcaccgag
     1381 gagcaattgg cctcggtcgt cgccttcgaa cgggtccgct tcggcggcgc cgatccagcc
     1441 gagaccctga ccgactgcgg tctggccccg gcggaaggcg aggagggggg cgctcctggc
     1501 gagaccacac ccgacgggga gtcgcccgcg gacggggaga cgacgaccac ggtgagcggc
     1561 gcggaagccg ccgcggcacg ggtgccctga cccccggcca gagatttttt ttctaggttc
     1621 tcgatctccg caaggcccgg cgcaatgccc ccgcctcgat ccttccctcg gccaggacga
     1681 cgtctcccgg cccgagcagg acgggcacac gcaacccata atccctggtc agagcatcat
     1741 ctgcgtcgat atcgatctca ttgagggcgg cacctacccg cgccaccgcc ttctcgacta
     1801 ccggccgcgc ctcgtcgcag agatggcatc cgggccgaat caggaatctg aacgcagaat
     1861 tgttcaagta accaccgtcg gtgaccgata atggacacgc atgcaagaag gtgggtcaac
     1921 cgttcccctc atacctaggg cacgttcgtc caaggtgtga acggatagcc tcccagcccg
     1981 ccccacggta cggaaccccc ttcgtgtgta gatgaggccc ccgccccggc gggggcctca
     2041 tcgtttttcg acacacaaac ggggtctgcc atcgtgtggg agttgagacc tgccaccgcc
     2101 gtcgtcctgg tcatcctcct gcttctcatc gccgtcgcgg ggatcatcca gttgacgtcg
     2161 atcctgtccc cgccagggtg actgccaccc ccgccgagag gttcaactcc gcatcggcgg
     2221 cgccgtcccg cacagcgatc gcaatcatcc ctgccgagtc gacatgcacc agcggctcac
     2281 ccggttccac ttcggagtag gcgacagccc acaggatggt gtggagagtc gcgcccacct
     2341 tcatcgtcaa caccgacccc ggtgagagcc cgaccgcggc cagatcctcg ggcccgatgt
     2401 tggtctggac gttcccgaac cgatccaccc accaggcctc gccggtgacg agagaccctt
     2461 gggtctcggg cagagggagc agcaacgact gcaccgcatc cggccccacc tcggggccca
     2521 ggtcctggat cgccgcctca cccgatgcga ggagagccgc cgcgggggcg aacacatcgc
     2581 ggccatgaaa ggtctctcct ggggagggaa tccttgcttc cgggttgtcg atggagacca
     2641 tgacccgggc cccgcccacc atcgccaccg caggcgacag gactccgttg tccgggccta
     2701 cgaagaatcc ccagtccgtc tccagtgcca gcgccagccg gtctgagccg acccccgggt
     2761 cgaccacgac gagggcgaca ccctcgggca gatactggat cgcccgggtc aggctgaggg
     2821 cggcgcccct cacatcgccc ctcgccgatc cgtggcccac gtcgatgact cgtgattccg
     2881 gagccagctt ggccagcacc ccatgcacga cgccgacgaa ctcgtcggca tgcccgaagt
     2941 cggagaggaa gctgatcggg agtgcgttgt gcttcatgcg tggtcctata ttgccgcgat
     3001 gtcggagacc agccacgacg gagtcgcgcg ggaggtgtcc cgggcaaggg tggcgatgct
     3061 catgggcagc gtcgacaccg gaaagacgac cctggctctc caggcggccc gtctcgccct
     3121 tggcgccggg cgaacggtcg cgatcgtcga cgccgacgtg ggcaactcga ccatcgggcc
     3181 tccggcgtgt gtcggattga aggtgctccg ttccccggac gacctcgacg acgtgaacac
     3241 ac
//
LOCUS       DASTXO010000383         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_907430, whole genome shotgun sequence.
ACCESSION   DASTXO010000383 DASTXO010000000
VERSION     DASTXO010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907430"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VFU96_08045"
     CDS             <1..787
                     /locus_tag="VFU96_08045"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEU5113258.1"
                     /translation="NAARGVDMRMGVEVESLEQDGGSAVVDFTDGSSGEFDLLVGADG
                     LNSSVRRHMSGKSAPSLVGQVSWRFITAGYAELSNWTVMLGGEKTFLAVPLGGGRVYC
                     YLDVSSHDSTDPTEGDPGRLEEMFGEFAAPATAFIRAATEAGSVHFGVIEEVVTDDWV
                     TGRVVLVGDAAHATSPNMAQGAAMAFEDAVVLAECLTDQATIPQALETYVRRRLPRIR
                     WIRRQTHRRDRIRSLPATVRNLSLRLAGNRIYRSNYAPLMTEP"
     gene            complement(789..>2102)
                     /locus_tag="VFU96_08050"
     CDS             complement(789..>2102)
                     /locus_tag="VFU96_08050"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEU5113259.1"
                     /translation="QIAYIVNHAGDRVLFVDPDLAPLLGPLTPELKTVETFVVMGESA
                     SDAIPGSIAYEDLIGDAEPHGHWPQLDERSPMMFCYSSGTTGHPKGVAYTQRSTYLHT
                     ISNLANFNMLPSDNVLPVVPMFHAAAWGYPFMAVTQGAKLTYPGPDLTPQGLVPLLER
                     EKVTFSAGVPTVWLGIKQYLEANPEVDLSSIRAFLCGGSAVPRSMIEWYWKVQGIRVV
                     QGWGMTETNPVASTSLLKPEMEEWDLDRQLDFLETAGIPVAGLQVKIVDEIGEELPHD
                     GEAFGELLIRGPWIAAEYYQDTRSAQSFEDGWLRTGDVCKITPEGYIKITDRAKDVIK
                     SGGEWISSIDLENALMAHPDVSEATVVGLKHVKWQERPVAFVVLREGVDVSEDDLNSF
                     LEERVAKWWLPDKILFIDQIPKTGTGKFDKKVVRNEYADLLMEEV"
BASE COUNT          374 a          651 c          682 g          395 t
ORIGIN      
        1 gaacgcggca cgcggcgtcg acatgcgtat gggtgtcgag gtcgagtcgc tggagcagga
       61 tgggggctct gcagtggtcg atttcaccga cggaagcagt ggcgaattcg atttgctggt
      121 cggggccgac ggcctcaact cctcggtccg caggcacatg tcggggaagt ctgcgccgag
      181 cctggtaggc caggtcagct ggaggttcat caccgctggt tacgccgagc tcagcaactg
      241 gactgtcatg ctcgggggcg agaagacgtt cctggccgtc cccctcggtg ggggccgcgt
      301 gtactgctac ctcgatgtgt cgagtcacga cagtaccgac ccgaccgaag gcgacccggg
      361 ccgtctcgag gagatgttcg gcgagttcgc cgccccggcc accgccttca ttcgggcagc
      421 aacggaggct ggctccgtcc atttcggagt catcgaagag gtcgtcaccg acgattgggt
      481 gaccgggcgg gtcgttctgg tgggtgatgc ggcccatgcc acgtcaccga acatggcgca
      541 aggagccgcc atggctttcg aagacgccgt agtccttgcc gaatgcctca ccgatcaggc
      601 gacgatcccg caggccttgg agacatatgt caggcgccgc ttgccacgca tccggtggat
      661 caggagacaa acgcaccgca gggacaggat caggtcgctt cccgcgacgg ttcgcaatct
      721 ctcgctccgg cttgcgggga atcggatcta tcgctccaac tacgcgccgc ttatgaccga
      781 accgtgagtc agacctcctc catgaggagg tcggcgtatt cgttcctcac cactttcttg
      841 tcgaacttgc cggtgcccgt cttcgggatc tggtcgatga acaggatctt gtcgggcagc
      901 caccacttgg cgacccgctc ttcgaggaac gagttcaggt cgtcctcaga cacatcgacg
      961 ccttcgcgga ggactacgaa cgccacgggg cgctcctgcc acttgacgtg cttgagaccg
     1021 acgacggtcg cctcgctcac atcagggtgc gccatgaggg cgttctccag gtcgatcgat
     1081 gagatccact ccccgcccga cttgattaca tctttggcgc ggtcggtgat cttgatgtag
     1141 ccctcggggg tgatcttgca gacgtcgccg gtgcggagcc acccgtcctc gaacgactgg
     1201 gcggagcgag tgtcctggta gtattcggcg gcgatccagg ggccgcggat gagcagctca
     1261 ccgaacgcct ccccatcgtg gggcagttcc tctccgatct catccacgat cttgacctgc
     1321 agccccgcca ccgggatgcc agcagtctcg aggaagtcga gctgccggtc caggtcccac
     1381 tcctccatct ctggcttgag gaggctggtg gacgcgacgg gattggtctc ggtcatgccc
     1441 cagccttgca cgacccgaat tccctggacc ttccaatacc actcgatcat cgaacggggc
     1501 accgccgatc caccgcagag gaaggcgcgg atcgaggaga ggtccacctc cgggttggct
     1561 tcgagatact gcttgatccc cagccagacg gtgggcactc cggcgctgaa ggtcaccttc
     1621 tcccgctcga gcaggggcac caggccctga ggggtgaggt cgggaccggg ataggtgagt
     1681 ttggcgccct gggtgaccgc catgaagggg tagccccaag ccgcggcgtg aaacatcggc
     1741 acaacgggca ggacattgtc actggggagc atgttgaagt tggccaggtt ggagatcgtg
     1801 tgcagatacg tggatcgctg cgtgtaggcg actcccttgg gatggccggt cgtgcccgag
     1861 gagtaacaga acatcatcgg cgaccgctcg tcgagctggg gccagtgacc gtgaggctcg
     1921 gcgtccccga tcaggtcttc ataggcgatc gatcccggaa tcgcgtccga cgccgactcc
     1981 cccatcacca cgaaggtctc cactgtcttc aactcgggtg tgagcggtcc aagcagtggg
     2041 gcaagatcgg gatccacgaa caacaccctg tcaccggcgt gattgacgat gtaggcgatc
     2101 tg
//
LOCUS       DASTXO010000384         2447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_687002, whole genome shotgun sequence.
ACCESSION   DASTXO010000384 DASTXO010000000
VERSION     DASTXO010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687002"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1513
                     /locus_tag="VFU96_08055"
     CDS             <1..1513
                     /locus_tag="VFU96_08055"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU5113260.1"
                     /translation="AAPKPKPVPMPQTDTATSGYKLPPLSLLDEGTGGEINKRALEDT
                     ARQLEETLIQHGVDANLTKIVPGPTVTRYEIELAPGVKVNRVSSLSHDIAYALATPDV
                     RILVPIPGKSAIGVEVPNTKRRLVSLGDILSSAEAAVDTHPLTVGLGMDISGLPKLLN
                     LSELPHVLIAGATGAGKSSCINSIVTSLLMRTHPDDVRVIMVDPKRVELGQYNGVPHL
                     LTRVITNPKKANDALKWAVAEMDRRYDLLADAKVRDIEGYRLKWEAGGLDPEGFDRFP
                     YIVILIDELNDLMMVAGREVEESIVRIAQMARAVGIHLVIATQRPSVDVITGVIKANI
                     PSRLAFSVASQTDSRVIIDGAGAEKLIGMGDMLVVTAKEPRPHRIQGAWVRESEVHAV
                     VDWVRKQQEAKYEDQEVLVAAAAAAAAQAEEVDDEEEALVRQAMDLVVRSELGSTSML
                     QRKLRIGFARAGRVMDILESRGVVGPSHGSTARDVLMTVDEMEEVLAAQGAGV"
     gene            1569..>2447
                     /locus_tag="VFU96_08060"
     CDS             1569..>2447
                     /locus_tag="VFU96_08060"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU5113261.1"
                     /translation="MLTLISPDGTVTDRLPITLDEMRRLYSDMVEARTYDHKCMALQR
                     QGRLATYAPFEGQEAAQIGAAAALRSDDWVAATYRDAALMWRAGYPWSLLVAGRTGDE
                     RGGQPPEGVNVLPPSITVGGHMIHAVGLAWAEALNGSDRIALTSFGDGATSEGDFHEA
                     MNFAAVYNTPTVFLCQNNGFAISYPRAEQTRSESIAIKAEGYGMPGVLVDGNDVAAVL
                     DAVGSAVARARVGEGPTLIEAVTYRLGPHTTSDDPGRYRDEDEAEGWRERDPLQRVRL
                     LLERAGGWDPGWQSEIE"
BASE COUNT          462 a          796 c          815 g          374 t
ORIGIN      
        1 cgccgccccc aagccgaagc cggtcccgat gccgcagacc gatacggcga ccagcggata
       61 caagcttccc ccgctcagtc tcctcgatga gggaaccggc ggcgagatca acaagcgagc
      121 gctcgaggac accgcccgcc agctggagga gacgctgatc caacacggcg tcgacgccaa
      181 cctgaccaag atcgttcccg ggcccacggt caccagatat gagatcgagc tcgcaccagg
      241 agtgaaggtg aatcgggtct cgagcctctc gcacgacatc gcctatgccc tggcgacccc
      301 cgatgtgcgc atcctcgttc caataccggg caagtcggcg atcggggtcg aggtccccaa
      361 caccaaacgc cgtttggtgt ccctcgggga catcctctcc agcgccgagg cggcggtcga
      421 cacccatccg ctgaccgttg ggctgggcat ggacatctcg gggcttccca agctgctcaa
      481 cctttcggag ctgcctcacg tcctcatcgc tggcgccacc ggtgccggga agtcctcctg
      541 catcaactcg atcgtcacct cgttgttgat gaggacccac cccgacgacg tgcgggtgat
      601 catggtggac cccaaacgcg tcgaactggg ccagtacaac ggcgtgcctc atctgctgac
      661 cagagtcatc accaacccga aaaaggcaaa cgatgccttg aagtgggcgg tggcggagat
      721 ggatcgccgc tacgacctcc tcgccgacgc caaggtgcgc gacatcgagg gttatcggtt
      781 gaaatgggag gccggcggcc tcgatccaga aggattcgac cgtttcccct acatcgtgat
      841 cctgatcgac gagctgaacg acctcatgat ggtggcgggg agggaagtcg aagagtcgat
      901 cgtccgcatc gcgcagatgg cgcgggcggt cggaatccac ctggtcatcg ccactcagcg
      961 tccatcggtc gacgtgataa ctggtgtcat caaggccaac atcccgagcc gtctcgcctt
     1021 cagcgtggcc tctcagaccg acagccgggt catcatcgac ggtgcggggg ccgagaagct
     1081 gatcggcatg ggcgacatgc tggtggtcac ggccaaggaa ccacgcccac accgcatcca
     1141 gggggcctgg gttcgggaga gtgaggtcca cgcggtcgtc gattgggtgc ggaaacagca
     1201 ggaggccaag tacgaggatc aggaggtcct ggtggctgcg gccgccgccg cggccgctca
     1261 ggcggaagaa gtcgacgacg aggaggaggc cctggtccgc caggccatgg acctggtggt
     1321 ccgcagtgag ctcggctcca cgtcgatgct gcagcggaag ctgcgcatcg gcttcgctcg
     1381 ggcgggacgg gtgatggaca tcctcgagag tcgtggagtc gtggggccgt ctcatgggag
     1441 cacggcccgc gacgtcctca tgaccgtcga tgagatggaa gaggtgctcg ccgcccaagg
     1501 cgccggggtc taggccgctt ccggccccaa ccggaatgaa ccgggcggga gataagctct
     1561 ccgccggcat gctcaccctg atcagcccgg acgggactgt caccgatcgc ctccccatca
     1621 ctctcgacga gatgcggcgg ctgtacagcg acatggtcga ggcccgcacc tatgaccaca
     1681 agtgcatggc gctgcagcgg caggggcgac tggccaccta tgccccattc gagggccagg
     1741 aggcggcaca aatcggggcg gcggccgccc tgcgatcaga tgactgggtc gccgccacct
     1801 accgcgatgc cgcactcatg tggcgggccg gatatccctg gagcctcctg gtcgcgggtc
     1861 ggaccggcga cgagcggggg ggacagccgc ccgagggggt caacgtcctc cccccgtcca
     1921 tcaccgtggg cgggcacatg atccacgccg tgggactggc ctgggccgaa gcgctcaacg
     1981 gatccgaccg gattgcgctg acatcgttcg gtgatggagc cacgtcggag ggagacttcc
     2041 acgaagcgat gaacttcgcc gcggtctaca acacaccgac cgtcttcctc tgccagaaca
     2101 atggctttgc catctcctac ccccgagcag agcagacacg ctccgagtcg attgcgatca
     2161 aggctgaggg ctacggcatg ccgggagtcc tcgtcgacgg aaacgatgtg gcggccgtcc
     2221 tcgacgcggt cggttccgcc gttgccaggg cccgcgtcgg cgaaggcccg accctcatcg
     2281 aagcggtgac ctaccgactg gggccacaca cgacttcgga cgacccgggc cgatatcgag
     2341 acgaggatga agcagagggc tggcgagagc gcgatcccct gcagcgggtg cggctcctac
     2401 tcgagagggc aggcggctgg gacccgggat ggcagagtga gatcgag
//
LOCUS       DASTXO010000385         3232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_870976, whole genome shotgun sequence.
ACCESSION   DASTXO010000385 DASTXO010000000
VERSION     DASTXO010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870976"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..265)
                     /locus_tag="VFU96_08065"
     CDS             complement(50..265)
                     /locus_tag="VFU96_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113262.1"
                     /translation="MNLAIGFPCPKCGHEEANLLAFERNRNAEVVGLRLLCTKCESVF
                     RENGLVDDAVPVDVSAHSGKGARSWVA"
     gene            complement(386..1669)
                     /locus_tag="VFU96_08070"
     CDS             complement(386..1669)
                     /locus_tag="VFU96_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016470645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEU5113263.1"
                     /translation="MGAHAELFERHKAVLPGWAPLYYEQPIAIVDGDGRYVTDAEGNQ
                     YLDFFGGILVTMSGYKVPEVVAAIKAQADKMLHTSTLYLIESQIELAEKIAGLSGIPD
                     AKVFFTNSGTEANDAALMLATTYKQSNQVLAIRGSYHGKSHSAIAVTGQRAWSGTSFS
                     PFQVTYVHGGYKLRSPFGHLPDEEFIAACVADLRDLLEVAAAGSPAVMIAEPIQGVGG
                     FVTPPDGFFKAMKDVLDENGILFISDEVQTGWGRTGEHFWGYQAHGVIPDLLTFAKGI
                     GNGLSIGGVVAPAEIMDSYPALGISTFGGNPLATAGALANLDFLLDNDLQTNALKVGN
                     RLLQNLVPLSEELELIGEVRGKGLMIGLELVQPDGSPSPEAAATVLEAAREEGLLIGK
                     GGLYGNSLRLAPPLSITEEEADEGFARLERALRKA"
     gene            complement(1687..2412)
                     /locus_tag="VFU96_08075"
     CDS             complement(1687..2412)
                     /locus_tag="VFU96_08075"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEU5113264.1"
                     /translation="MLRRTESGEPVDDLVGLLMGDERKAEGRPWVVVNFISSADGGTA
                     IAGKSSSLGDPDDKAVFQALRAVPDVILVGSGTVEAEDYRPVALDEERRERRLAAGLT
                     EVPVLAIVSGRLSFDTEARVFSDPEHRPMVVTGPRADPVKLAMLGDSADVVILEDVTP
                     AAILARLSAAKVILCEGGPTLVGQFVADRMIDELALTIAPLMIGGRSARIAHGETADP
                     PVEMRLDRQLIGDRSLFMRYLKN"
     gene            complement(2429..>3232)
                     /locus_tag="VFU96_08080"
     CDS             complement(2429..>3232)
                     /locus_tag="VFU96_08080"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEU5113265.1"
                     /translation="DGTKEFHLTAAITDWEVEPGKMVEAWTYNGMVPAPKIQVEVGDK
                     VRVVVENQLPMGTDVHWHGVKVPNNMDGVAPLTQPLIKPGESFVYEFTTREPMVAMYH
                     AHHHGQMQVPNGLFGTFMVGEVAMPVGQTISGVEIPADLEVSQEIPMVLNDAGVIGLS
                     LNGKSFPATQPYTGKVGDWVVVHYYNEGLQIHPMHQHQFRQLVYAKDGIPLDNPYWVD
                     TLNVAPGERYSVLMQLEDPGVWVWHCHILTHVERDEGMYGMVTAFIVEE"
BASE COUNT          590 a         1079 c          960 g          603 t
ORIGIN      
        1 gacctacacc gcctgctcta acggtcgcct acgaccagcg ggtcgttgct catgcaaccc
       61 acgatcgggc tcctttcccg ctgtgtgccg acacgtcgac cggcaccgcg tcatcgacga
      121 gaccgttttc acggaacacc gattcgcact tcgtgcagag gagtcgaagg ccaaccactt
      181 cggcgttccg attcctctcg aatgcgagga gattggcttc ctcgtgccca catttggggc
      241 aagggaatcc gatggcaaga ttcatgtagt gatccctcca gatggtcgtg gccctcacga
      301 cgcacctgaa tgatgtgtcg caggagcgcc atccggatag ggggatcaac cctgggggaa
      361 atacccctat gtggcgctct gctccttacg ccttgcgaag ggcccgttcc agccgggcga
      421 agccctcgtc ggcctcttct tcggtgatgg agagaggcgg tgcgaggcga agcgagtttc
      481 cgtagaggcc gcctttgccg atgagaagcc cctcctcccg cgctgcctcc agcaccgtcg
      541 cggccgcctc cggcgaggga gagccgtcag gctggaccaa ctcgagcccg atcatcagac
      601 ccttcccccg aacctcgccg atgagctcga gctcctccga cagcggtacc aggttctgga
      661 ggaggcggtt ccccaccttc aaggcgttgg tctgcagatc gttgtccagc agaaagtcga
      721 gattggccag ggcccccgcc gttgccaacg gattgccgcc aaaggtcgag atgccgagcg
      781 ccgggtaaga atccattatc tcggcgggtg ccaccacacc tccgatgctg agaccattgc
      841 cgatgccctt ggcaaaagta agtagatcgg ggatgacacc atgggcctgg tacccccaga
      901 aatgctcgcc ggtccgcccc catccggtct gcacctcgtc cgagatgaag aggatgccgt
      961 tctcgtcgag cacgtctttc atcgctttga agaaaccatc gggcggcgtc acgaagccgc
     1021 ccacaccctg tatcggctcg gcgatcatca cagccgggct gccggctgcg gccacctcca
     1081 gcaagtcccg caagtcagca acgcaggctg cgatgaactc ctcgtcggga agatggccga
     1141 atgggctccg caacttgtac ccgccgtgga cataggtcac ctggaacggc gagaaactcg
     1201 tccccgacca ggccctctgc ccggtcaccg cgatcgcgga gtgcgacttc ccgtgatagc
     1261 tgcccctgat cgccagcacc tggttcgatt gcttgtaagt cgtcgccagc atcaaggcgg
     1321 cgtcgttggc ttcggtgccg gaattggtga agaagacctt ggcatcgggg attccggaca
     1381 gcccggcgat cttctccgcc aactcgatct gcgattcgat gagatagagc gtcgaggtgt
     1441 gcagcatctt gtcggcctgc gctttgatcg ccgccaccac ctcagggacc ttgtacccgg
     1501 acatcgtgac caggatcccg ccgaagaaat cgaggtattg attgccttcg gcgtccgtga
     1561 catagcggcc gtcgccgtcg acgatcgcga tcggctgctc gtaatagagc ggggcccagc
     1621 cgggcaacac tgccttgtgt cgttcgaaaa gctcggcgtg cgctcccatc gtcgtctccc
     1681 ttggcgtcag ttcttcaaat atcgcataaa caaggatcgg tcgccgatca gttgccggtc
     1741 gagcctcatc tcgacgggcg gatcggctgt ctccccgtgg gcaattcgcg ccgacctgcc
     1801 gccaatcatg aggggggcga tggtcagcgc gagctcgtcg atcatgcgat cggcgacgaa
     1861 ctggccgacc aaagtcggcc cgccttcgca gaggatgacc ttggccgcac tcagacgggc
     1921 cagaatcgct gccggagtca cgtcctcgag gatgaccacg tcggctgaat cccccagcat
     1981 ggccaatttg accggatccg ccctcggccc cgtgacaacc atcggccggt gctccgggtc
     2041 ggagaacacc cgtgcctcgg tgtcgaaact gagcctgccg gaaacaatgg ccaggaccgg
     2101 gacttcggtc agaccggccg cgagtcgtcg ctctcgacgt tcctcgtcga gggcgaccgg
     2161 ccggtagtcc tctgcctcca ccgtccccga cccgacgagg atgacatcgg gcaccgctct
     2221 cagggcctga aaaaccgcct tgtcgtcggg gtcgccgaga ctcgaggact ttcctgcgat
     2281 cgccgtcccc ccgtcggcgg agctgatgaa attgacgacc acccagggcc ggccctccgc
     2341 ctttcgctca tcacccatga ggagccccac gagatcatcg acaggctcgc cggattcggt
     2401 cctccgaagc atcggcggga cggtagccct actcctcgac gatgaaggcg gtcaccatcc
     2461 cgtacatgcc ctcgtcccgc tccacgtgag tgaggatgtg acaatgccag acccagacac
     2521 cgggatcctc gagctgcatc aggaccgagt agcgctcacc cggggcgacg ttcagcgtgt
     2581 ctacccaata gggattgtcc aggggaatgc cgtccttggc gtagaccagc tgacggaact
     2641 ggtgctgatg catcgggtgg atctgcagac cctcgttgta atagtgcacc acgacccaat
     2701 cgcccacctt ccccgtgtag ggctgggtgg caggaaagga cttgccgttg aggctgaggc
     2761 cgatcacgcc ggcgtcgttg aggaccatgg gaatctcctg ggaaacctcg aggtccgccg
     2821 gaatctcgac accggagatg gtctggccca caggcatagc cacctcgccg accatgaacg
     2881 tcccgaacaa cccgttggga acctgcatct gcccgtgatg atgggcgtgg tacatcgcca
     2941 ccatcggctc tctcgtggtg aactcgtaga cgaagctctc acccggcttg atcaatggct
     3001 gggtgagcgg ggccaccccg tccatgttgt tcgggacttt caccccgtgc cagtggacgt
     3061 cggtgcccat cggtagctgg ttctccacga ccacccgcac cttgtccccc acctcgacct
     3121 ggatcttcgg cgccggcacc atcccgttgt aggtccaggc ctcgaccatc ttgcccggct
     3181 cgacctccca gtcggtgatc gcggccgtga gatggaactc cttggtcccg tc
//
LOCUS       DASTXO010000386         3320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1607370, whole genome shotgun sequence.
ACCESSION   DASTXO010000386 DASTXO010000000
VERSION     DASTXO010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607370"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /gene="ccsA"
                     /locus_tag="VFU96_08085"
     CDS             <1..503
                     /gene="ccsA"
                     /locus_tag="VFU96_08085"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEU5113266.1"
                     /translation="DRIAEASVETGVLFTATGLFTGMVWGQAVWGRAWDWADPRMAST
                     AVMFFVYIGYLALRRATPDPVMRARRSAILGAIAVVQVPLVYFSVNLFRSLHQTQSIR
                     PDGATMPDEMVIALLVNFLAFTLLYLALTAARVGVARVEEAKSGEAVPAGDAVSTPLL
                     SEVEDV"
     gene            603..1010
                     /locus_tag="VFU96_08090"
     CDS             603..1010
                     /locus_tag="VFU96_08090"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEU5113267.1"
                     /translation="MVHRRFVYLGIGGVIAIVLALTFTGLNDSLTYYLYPSEAVDQRA
                     DFPDGERFRLAGIVVAGTVVEGDTSTSFDVTDGGADIPVVLSGPKPSLFAEEVPLLLE
                     GAWSGDTFEADSALIRHDENYEVPEEGGAYPEG"
     gene            1010..3019
                     /locus_tag="VFU96_08095"
     CDS             1010..3019
                     /locus_tag="VFU96_08095"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HEU5113268.1"
                     /translation="MLALLGSAGVIAALVGSILLVSRGVRLARGGVGDLRAPGKLVLA
                     GAVISMVAMQVALLTDDFSLAYVANHHSSNTPFPFDVATAWAALEGSIILWGLVLAIF
                     TWVVARREPDRLGAAAVAVMGVVGIFFFGLMVTVANPFEVCVDALATGCAASTAWPCN
                     PVDVPGNGPGPNPLLQNHFLMAVHPPVLYIGYVGLTVPFAYAIGALALGVPGPEWLRR
                     SHRSTLVAWSFLTLGITLGGWWAYEVLSWGGYWAWDPVENASLMPWLAATAFIHSGLV
                     QQRRGMLQAWNFVLVISAFALTILGTFLTRSGTINSVHSFTQSAIGPALLGFLVLVLV
                     GSFTLFSMRSHLVATSPRIETFVSREGTFLVNNLLLTVYAFVVLIGTTYPLLLEAFTG
                     TQVGVGEPFFNRLAVPLSFMLLLTMGFGPVTPWGGAPPGLLWRRLRWPTMVALALGLV
                     VAMTVTRVAWVILAIVLGAFVAAAIFGLLLELARARTAKSGHSLYQEAKTVIRNDQTF
                     WAGQLSHIGVVLVAVGIAFAANLPLHSEVELIPGQSVEFAGYTLTYDAPFRREEPNRV
                     VQGARVEVSRDDRIITVLEPRANFYDTEMGGIVTPAVMSRPGGDLYLTLRNLDDETVQ
                     LGLDTSPMIWLLWLGGITTAAGGFWSLTVRRRARISEEQRVLADV"
     gene            3012..>3320
                     /locus_tag="VFU96_08100"
     CDS             3012..>3320
                     /locus_tag="VFU96_08100"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HEU5113269.1"
                     /translation="MSDRARNIASIAFIVVMAVALILITSTRPAAVDRVEQIGTRIKC
                     PVCQGESIANSPSQMAEDMMDLVSERVSAGVPDEEIIDELLGSYTGAVLLDPPASGAT
                     L"
BASE COUNT          483 a         1107 c         1053 g          677 t
ORIGIN      
        1 gggatcgaat tgcggaggcg tcggtggaaa ccggcgtgct gttcaccgcc accggcttgt
       61 tcaccgggat ggtgtggggg caggcggtgt ggggacgggc ctgggattgg gccgatcccc
      121 gcatggcgtc gacggcggtc atgttcttcg tctacatcgg gtacctggcg ttgcgacggg
      181 caacgccaga cccggtgatg cgggcgaggc gctcggcgat cctcggcgcc atcgctgtgg
      241 tccaggtgcc tctcgtctac ttctctgtca acctttttcg ttccctccac cagacccagt
      301 cgatccggcc tgacggggcc acgatgccgg acgagatggt gatcgccctg ctcgtcaatt
      361 tcctcgcctt cacgctgctc tatctggctt tgaccgcagc ccgcgtcggg gtggccaggg
      421 tcgaggaggc aaagtcgggc gaggccgtcc cggcggggga cgcagtgtcc actcccttgc
      481 tgtccgaggt cgaggatgtc tgaccaggtc atcgtcttcg gcaccttctt catctgctac
      541 tccgccttcg tctggtacgc agcccgcctc catctgcgct atcggcgagc caagggctga
      601 cggtggttca tcgccgattc gtctacctcg gcatcggggg ggtgatcgcc atcgtgctgg
      661 cactgacctt caccgggctg aacgacagcc tcacctacta cctctatccg tcagaggctg
      721 tcgaccaacg ggccgacttc cccgacgggg agcggttccg gctcgccggc atcgtcgtag
      781 ccgggaccgt cgtggaaggt gacacgtcga ccagcttcga cgtgaccgac ggcggagcgg
      841 acatcccggt ggttctcagc ggccccaagc cgtcgctgtt cgccgaggaa gtccccttgc
      901 ttctcgaagg tgcctggtcc ggcgacacgt tcgaggcgga ctcggcgttg atccgccatg
      961 acgagaacta cgaggtcccc gaggagggcg gggcctaccc ggaggggtga tgctggccct
     1021 tctcggatcg gccggagtga tcgcagccct cgtcggttcg atcctgctcg tgtcgagggg
     1081 cgtccgcctc gcccgtggtg gtgtgggtga cctgcgcgcg cccggcaagc tcgtcctggc
     1141 aggagcggtc atttccatgg tcgcgatgca ggttgcgctg ctcacggatg acttcagtct
     1201 ggcctacgtg gccaatcacc actcgagcaa taccccgttt cccttcgatg tcgccacagc
     1261 ctgggccgcc ctcgagggct cgatcatcct gtgggggctg gtcctggcca tcttcacctg
     1321 ggtggtcgcg cggcgggaac ccgatcgtct gggagcagca gcggttgccg ttatgggggt
     1381 cgtaggcatc ttcttcttcg ggctcatggt caccgtggcc aacccgttcg aggtgtgcgt
     1441 cgatgccctg gccaccggat gcgcggcctc gaccgcatgg ccctgcaacc cggtcgacgt
     1501 gccgggcaac gggcccgggc caaatccatt gctccagaac cacttcctga tggcggtcca
     1561 cccgccggtg ctgtacatcg gctacgtcgg gctcaccgtg ccattcgcct atgcgatcgg
     1621 cgccctcgcc ctcggggttc ccggtcccga gtggttgcgc cgcagccatc gctcgaccct
     1681 ggtcgcctgg tcgttcctga ctctggggat caccctcggc ggctggtggg cgtacgaagt
     1741 cctctcctgg ggcggatact gggcttggga tccggtagag aacgcctccc tcatgccctg
     1801 gctcgctgcc acggcgttca tccactctgg gttggtccaa caacggcgag ggatgctcca
     1861 ggcatggaac ttcgttctcg tgatcagcgc cttcgcgctg acaatcctcg gcaccttcct
     1921 cacccgatcg ggcacgatca actcagtcca ctcattcact caatccgcga tcgggcccgc
     1981 gcttctcgga ttcctggtcc tggtcctggt tgggtccttc actctgttct ccatgaggtc
     2041 gcacctggtg gcgacctcac cccggatcga gaccttcgtc tctcgtgagg gcaccttcct
     2101 ggtcaacaac ctgctgctga ccgtctacgc gtttgtcgtc ctcatcggaa ccacctaccc
     2161 actcttgctc gaggccttca cggggaccca ggtgggagtt ggagagcctt tcttcaaccg
     2221 cctcgcagtg cctctgtcct tcatgctgct cttgacgatg gggttcggcc cggtcacgcc
     2281 gtggggtgga gcgccgccgg gcctcctctg gcgccgcctt cgatggccga cgatggtcgc
     2341 actggctctc ggtttggtgg tcgccatgac cgtgactcgg gtcgcctggg tgatcctcgc
     2401 catcgtcctt ggagcattcg tggcagcggc gatcttcggc cttcttctgg agctcgcccg
     2461 agcccggacc gccaaatccg ggcattcgct ctaccaggag gcaaagaccg tcatcaggaa
     2521 cgatcagacg ttctgggcgg gccagctcag ccacatcggt gtcgtcctcg tcgcggtcgg
     2581 gatcgcgttc gccgccaacc tccccctcca ctccgaggtg gagctgatac cgggccagtc
     2641 ggtcgagttc gcgggttaca cactcaccta cgacgcgcca ttccgccgcg aggagcccaa
     2701 ccgtgtcgtc cagggtgccc gcgtcgaggt cagccgtgac gaccgcatca tcacggtgct
     2761 ggagccgcgg gcaaacttct acgacaccga gatggggggc atcgtcacgc cggccgtgat
     2821 gagccggccc ggtggcgacc tctatctgac gctcaggaac ctcgatgacg aaacggtgca
     2881 gctcggtcta gacacgtctc cgatgatctg gctcttgtgg ctcggtggga tcacgacggc
     2941 cgccggtgga ttttggtccc tgacggtgag acgccgggcc cggatcagcg aagaacagcg
     3001 ggtcctggcc gatgtctgat cgagcccgca acattgcctc tattgccttc atcgtggtga
     3061 tggcggtggc gctgatactc atcacctcga ccagaccagc agcggtcgac cgcgtcgaac
     3121 agatcggtac ccggatcaag tgccctgtgt gccagggaga gtcgatcgcc aattcccctt
     3181 cacagatggc cgaagacatg atggacctcg tatcggaacg cgtctccgcc ggcgtgcccg
     3241 acgaagagat aatcgacgag ctgttgggct catacaccgg tgcggtactc ctcgacccac
     3301 cggcgtcagg cgccaccctt
//
LOCUS       DASTXO010000387         2559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1202522, whole genome shotgun sequence.
ACCESSION   DASTXO010000387 DASTXO010000000
VERSION     DASTXO010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202522"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..674)
                     /locus_tag="VFU96_08105"
     CDS             complement(93..674)
                     /locus_tag="VFU96_08105"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU5113270.1"
                     /translation="MIKPAFDRVIGLALLIVFSPVIAASALAVLVTMGGPVFYSQERI
                     GLGGRAFRLYKIRTMIPDRRVGQDAYIGAERRLVHKSVDDPRVTLVGKALRATRFDEL
                     PQFLNVVKGEMSMVWPRPELPEIVRRYEPWQHGRHLVKPGVTGPWQVSNHNGDLMYQH
                     TEIDLEYLEQISFAHDLGILIRTPMAMVGRKGH"
     gene            1127..>2559
                     /locus_tag="VFU96_08110"
     CDS             1127..>2559
                     /locus_tag="VFU96_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEU5113271.1"
                     /translation="MRQIAQNYKTGEVKLVDVPVPACRPGGVVVRSEYSLVSAGTELM
                     KIAESKMSLLGKARARPDQVRKVLESVSQQGVGATYRKVMNRLDSYTPLGYSLAGTVV
                     ESMVPELPVGSRVACGGNEYALHAEFNYVPRNLCALVPDGVKAEHAAFATVGAIAMQE
                     FRRSEATIGETVCVIGLGLIGQLLVQIADAAGVNVVGLDPSEERCGLAERSGARAAGA
                     PEGSSASRVRERLLDLTDGHGADVVFLAAATDSNQPVSTAVEIARDKARIVDVGKMPL
                     DLPWKPYYEKEIDVRFSRSYGPGRYDPRYEEEGFDYPIGYVRWTEQRNMRSFLELVGA
                     GKIQLESLVDEIIPFDSSVEAYERLLSGESRAIGSLFGYPQETSIERVLHLGKQADIA
                     PRTGKVGVGVIGSGNYASTMLLPHLASNPDVHLAAVATATALSGATAQQRFGFDRITS
                     DYRTLIDDGAIDAVIIATRHDLHASMVS"
BASE COUNT          495 a          777 c          800 g          487 t
ORIGIN      
        1 gatcgcgaag gtgcggtcct ccaagtcgtc gggtgacaaa gcaccaagcg atagccggct
       61 cacggctcga ttcttcgcgt ctccgatgcg catcagtggc cctttcggcc aaccattgcc
      121 atcggggtcc tgatcaggat cccgaggtca tgggcgaagc tgatctgctc gagatactcg
      181 aggtcgatct cagtgtgctg atacatcaag tcgccgttgt ggttggaaac ctgccatggg
      241 ccggtaacgc cgggcttgac cagatgacga ccgtgttgcc agggctcgta gcggcggacg
      301 atctcgggga gctcgggccg gggccacacc atgctcatct cacccttgac cacattcagg
      361 aattggggca gctcgtcgaa tcgtgtcgcc cggagcgcct tcccaaccag cgtcactctg
      421 ggatcgtcta ccgatttgtg gacgagccgt cgctcggccc cgatataggc atcctgaccg
      481 acgcgtcggt cagggatcat ggtcctgatc ttgtagagcc gaaaggcacg cccaccaaga
      541 ccgatccgct cctggctgta gaagaccggg ccccccatcg tcaccaatac cgcgagggcc
      601 gaagcagcga tcacagggct gaaaacgatc agcagtgcaa gaccgatcac tcggtcgaac
      661 gcgggtttga tcacgaccgg gtagagaccc gacggtgctg atgttcccct ctcaccccaa
      721 atttcgccgc ccaccggtaa tacgggcgca agcgcctcac ccttacgagc ccacatcgtc
      781 tcgtctcccc tgagttgtct ggatagcccc atggatctgt tcgatccagt ggcgatacta
      841 ccaccaccga gagtggtgag aagaggccaa gttcacgacc cccatggcga gtgcacgtag
      901 gagccgtggt gcgcaacaat tgcggctcgg tggttctcca gcctgaacct tcgggataat
      961 tccgtctggc gtgatgtgat catgtcggtc agaatgaacg gctgcgaggc tggatctggt
     1021 tacaggggag cggggttgtc cagcgtgggg gaagatttcg tttgcggggg ttttcgatga
     1081 tcgtgaggat gacgtctgtc ccgagtcggg gtcggaagga cctcagttga gacagatcgc
     1141 ccagaactac aagaccggtg aggtgaagct cgtcgacgtc ccggtgccgg cgtgccggcc
     1201 aggaggagtg gttgtccgca gcgaatactc cttggtctct gccgggaccg agttgatgaa
     1261 gatcgcggag agcaagatgt ctctcctcgg caaggcccgg gccaggccgg atcaagtcag
     1321 gaaagtgctc gagtcagtct cccagcaggg tgtgggcgcc acctatcgca aggtcatgaa
     1381 ccgcctcgac tcctacaccc cgctcggata ctcactggcg ggcaccgtcg tcgagtcgat
     1441 ggtccccgaa ttgccggtag gcagccgcgt cgcgtgtggc ggaaacgagt acgcactcca
     1501 tgccgagttc aactatgtgc cacggaacct gtgcgccctc gttcccgacg gggtgaaagc
     1561 cgagcacgcc gcctttgcca ccgtgggtgc tatcgcgatg caggaatttc ggcgctcaga
     1621 ggccacgatc ggcgagaccg tctgcgtgat cgggttgggt ctcatcggtc aactgctcgt
     1681 tcaaatcgcc gatgctgctg gcgtaaacgt cgttggtttg gacccgtcgg aggagcgttg
     1741 cgggctcgcc gagcgctccg gcgcccgtgc cgctggagca cccgaggggt cgtctgcatc
     1801 acgggtgcga gagaggcttc tcgatctcac cgatggtcac ggcgccgacg tggtcttcct
     1861 cgccgctgcc accgactcga atcagccggt gagcaccgcg gttgaaatcg ccagggacaa
     1921 ggctcgaatc gtcgatgtcg gcaagatgcc gctagatctc ccatggaagc cctactacga
     1981 gaaggagatc gatgttcgct tctcgaggtc ctacggcccg ggaagatacg acccgagata
     2041 tgaggaagaa ggtttcgact atccgatcgg gtacgtgaga tggaccgagc agcggaatat
     2101 gagatccttt ctcgaactgg ttggcgcggg caagatccag ctggagagcc tggtcgacga
     2161 gatcatcccc ttcgacagct cggttgaggc ctatgagcgg cttctctccg gagaatcgcg
     2221 cgctatcggc agtttgttcg ggtacccaca ggagacatcg atcgagcggg tactccacct
     2281 gggcaagcag gcggacatcg cgcccagaac cggcaaagtc ggcgtcggag tcatcggcag
     2341 cggcaactac gccagcacga tgttgctgcc acatctggcc tcgaaccccg acgtgcacct
     2401 agcggcggtt gcgaccgcca ctgccctctc cggcgccacc gcccagcaga ggttcggatt
     2461 cgaccggatc acgtctgact acaggaccct gatcgacgac ggtgccatcg atgccgtcat
     2521 catcgccaca cgccacgatc tccacgcttc gatggtctc
//
LOCUS       DASTXO010000388         2876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1534037, whole genome shotgun sequence.
ACCESSION   DASTXO010000388 DASTXO010000000
VERSION     DASTXO010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534037"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VFU96_08115"
     CDS             complement(<1..746)
                     /locus_tag="VFU96_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HEU5113272.1"
                     /translation="MRVYELARDLGVESKDLLQQAHDLGIEVKTASSGLDDESAELLR
                     LAVAGDEPEVAEAEPEAEVEAEEEATTEPIPEPEVELDIVSIPSGASVSEFADALGVP
                     AKDVVAQLLTRGQPVGAGQTMPPELIEEVAESFGFIVDIEAPPPPPAVAERPVFEDAE
                     SELAPRPAVVTVMGHVDHGKTTLLDTLRKTSVVEGEQGGITQHIGAYQVDVNGHPITF
                     IDTPGHEAFTSLRARGANITDIVVLVVAAN"
     gene            complement(760..1938)
                     /gene="nusA"
                     /locus_tag="VFU96_08120"
     CDS             complement(760..1938)
                     /gene="nusA"
                     /locus_tag="VFU96_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEU5113273.1"
                     /translation="MKLDTMMEAIEVLARERGVAVDTILDAFANALVAAYKRSPGAAE
                     EARVTIDPDSGEVIVYAQELDEDGNVVSEWEDTPEGEAFGRIAAQSFKQVMQHKLREA
                     KREHVFEMYEGREGDLVTGLVQQVDYRSVILDLGDAEAILPGSERIPFERLERGNRVK
                     ALILEVRNEAKGAQIVVSRSHPDLVRRLLELEVPELTDGTVEIVNIAREPGHRTKIAV
                     ISHDPNVDPKGACVGARGSRVRQVVNELRGEKVDVVQWRDDIGQFIAEALGPAKVKEV
                     HIDEETKTADVVVSEHQLSLAIGKEGQNARLAARLSGYKVDIRSDMEGVEVEGASPEG
                     IPAEQAPVESEVPGDEAAATLEEVVDGVPVEEAVAADVPASDDEDAVPTADDTDDESE
                     "
     gene            complement(1948..2418)
                     /locus_tag="VFU96_08125"
     CDS             complement(1948..2418)
                     /locus_tag="VFU96_08125"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEU5113274.1"
                     /translation="MTTVSDIWTVVEPYLAAERLELDDVELLGRGPGTLLRVVVDGED
                     VNIDRLAELSRGISRLLDNETELVDAYRLEVTSPGLERDLRRPAHYAKSVGREVVVKV
                     ADGDHKATIRGVLADASDSTFTVESDDERRLVDYEDVLTAKTVFRWEKAPKPGH"
     gene            complement(2588..>2876)
                     /locus_tag="VFU96_08130"
     CDS             complement(2588..>2876)
                     /locus_tag="VFU96_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HEU5113275.1"
                     /translation="NIEALDSLHEIGLPAGYDFSGMIEGGESGYFIDSISHVARIDVD
                     ETGTTAGAATDVAIAGSHGPTVPIDRPFFYFIRDRGSGAILFMGHVTDPRG"
BASE COUNT          455 a          979 c          905 g          537 t
ORIGIN      
        1 tcgttggcag ccaccaccag gacgacgatg tcggtgatgt tcgccccccg cgcccgcagc
       61 gaagtgaacg cctcgtggcc cggtgtgtcg atgaaggtga tggggtgccc gttgacgtcg
      121 acttggtagg caccgatgtg ctgggtgatg ccaccctgtt ctccctcgac cacactcgtc
      181 ttgcggagcg tgtcgaggag ggtcgtcttc ccatggtcca cgtggcccat gacggtgacc
      241 accgcgggcc ggggcgccag ctccgattcg gcgtcttcga acaccgggcg ctctgcaacc
      301 gccggtggcg gcggcggcgc ctcgatgtcg acgatgaacc cgaacgactc ggccacctcc
      361 tcgatcagct cgggcggcat cgtctgccct gctcccacgg gctggccgcg ggtcaacagc
      421 tgggcgacga cgtccttggc cgggacgccc aaggcatcgg cgaactcgga aacagaggcg
      481 cccgatggga tggaaactat gtcgagctcg acttcgggct ccgggatggg ctcggtggtg
      541 gcttcctctt cggcctctac ctccgcctcg ggctcggctt ccgccacttc gggttcgtct
      601 ccggcgacgg caagacggag cagctcagcc gactcgtcat cgagcccgga ggaggcggtc
      661 ttgacttcga tccccaggtc atgggcctgc tggagcaaat ccttcgattc gacccccagg
      721 tccctggcca actcgtaaac cctcacgtct ggaaaacccc tattcgctct cgtcgtccgt
      781 gtcgtcggcg gtgggcacgg catcctcatc gtcggatgca ggcacgtcgg cggcgactgc
      841 ttcctcaacc gggaccccgt cgaccacttc ctctaaggtc gcggctgctt cgtcgcccgg
      901 aacctcgctc tcgacggggg cttgctctgc cggaatccct tccgggctcg ctccctccac
      961 ctcgacgcct tccatgtcgg agcggatgtc gaccttgtat ccggagaggc gggcggcgag
     1021 acgggcgttc tggccctcct tgccgatggc gagagagagt tgatgctctg acacgaccac
     1081 atcggccgtc ttggtctcct cgtcgatgtg gacttccttg accttggccg ggcccagagc
     1141 ctcggcgatg aactggccaa tgtcgtcccg ccactggacc acgtccacct tctcaccacg
     1201 cagctcgttc acgacctggc gcacgcggga gcccctggcc cctacgcagg cgcccttggg
     1261 gtcgacgttc ggatcgtgag agatcacggc gatcttggtg cgatggccag gctcgcgtgc
     1321 gatgttgacg atctcgaccg tgccatcggt cagctccggc acctcgagct cgaggaggcg
     1381 acgcaccaga tccggatggc tgcgggagac cacgatttgg gcgcccttgg cctcgttgcg
     1441 cacctcgagg atcagcgcct tcacccgatt gccgcgctcg agacgctcga acgggatccg
     1501 ctcggagccc gggaggatcg cctcggcgtc cccgaggtcg aggatcaccg accgatagtc
     1561 cacctgctgg accagaccgg tcacgagatc gccctctcgc ccctcgtaca tctcgaacac
     1621 gtgctcgcgt ttggcctcac gcagcttgtg ctgcatgacc tgcttgaacg attgggcggc
     1681 gatgcggcca aaggcctcgc cttcaggcgt gtcctcccat tccgacacca cgttgccgtc
     1741 ctcatccagt tcctgcgcgt agacgatgac ctcaccggag tcgggatcga tggtgacgcg
     1801 cgcctcctcc gcagcccccg ggctccgctt gtaggcggcg accagggcgt tggcgaacgc
     1861 gtcgaggatg gtgtcgacag ccacgccccg ttctcgggcg aggacctcga tcgcctccat
     1921 catcgtgtcg agcttcatga ttctccttca gtggcccggc ttgggcgcct tctcccacct
     1981 gaaaactgtc ttcgcagtga gcacgtcttc gtagtcgacg agccgacgct catcgtctga
     2041 ctccacagtg aatgtcgagt cgctggcgtc ggccaggacg cccctgatgg tcgccttgtg
     2101 gtcgccgtct gcgaccttga ccacgacctc acgaccaacc gactttgcgt agtgggccgg
     2161 gcgacggagg tcccgctcca accccggtga ggtcacctcg agccggtagg cgtccacgag
     2221 ctcggtctcg ttgtcgagca gtcgggaaat cccgcgggag agctcggcca gacggtcgat
     2281 gttcacgtcc tccccgtcga ccacgacgcg caggagggtc cctggcccgc ggccgagcaa
     2341 ctcgacatcg tcgagctcga ggcgttcggc ggcgagatag ggctccacca ccgtccaaat
     2401 gtcggaaacc gtcgtcaaaa tgcctgctcc tggctccgtt gtgccaccgg ggtcgcactc
     2461 atcgcgcaaa aaatacaggc gggtgccaaa ggcgcccacc tggttcaaag cgtcctgttg
     2521 tccaatcgca ttataggcgg accgggcacc ttgtggtgtg ggctgatgcc cactagcagc
     2581 gggggcgtca gccccggggg tcggtcacat gacccatgaa caggattgca cctgagccac
     2641 gatcccggat gaaatagaag aacgggcgat cgatcgggac cgtcggcccg tgggatccag
     2701 caatggccac atccgtggcg gctccggcgg tggtcccggt ctcgtcgacg tcgatgcggg
     2761 cgacgtgcga gatcgagtcg atgaagtagc cggactcgcc gccctcgatc atcccactga
     2821 agtcatatcc ggccggcagc ccgatctcgt gtagagaatc gagcgcctcg atgttg
//
LOCUS       DASTXO010000389         2532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_797374, whole genome shotgun sequence.
ACCESSION   DASTXO010000389 DASTXO010000000
VERSION     DASTXO010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797374"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VFU96_08135"
     CDS             complement(<1..894)
                     /locus_tag="VFU96_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113276.1"
                     /translation="MTVPAPVAGGVGQSLLRRGWVRNLGRTVLVLAAVAGVPLLVVVL
                     TGGQSSVIYWIDVAMFSLFAYTIGFWPLWIVLVLPLGLITWLVGKFSQPAADRIGRYF
                     TPVVWILGGTSAFLVMGPTPTEGVALPTGHPRVGALLVLLGGSLLSAGLLVTVRRIQM
                     PTQDLAPLAITRSLTPLPWTAAPQTVEPTEEYWSPDAVVAWRAWQWTGSVLRGVVTEW
                     RTQRFVAWCDYCPEAPGWDHPCGIYGVPRPSEVAAMFPGKPDVVGRVELTGLVIEHES
                     GYRASRARILDLWTRSAFVADQ"
     gene            1005..1976
                     /locus_tag="VFU96_08140"
     CDS             1005..1976
                     /locus_tag="VFU96_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012183913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEU5113277.1"
                     /translation="MQAIVYDRYGPPDVLQLREVADPEIGATDVLVRVRAASANPLDW
                     FAFNGTPYIARPGFGLKKPNSTRLGADLSGVVAEVGSQVTRFVPGDEVFGAGTGSFAE
                     LATVGGTGAISSKPASLTFEQAAAVPVAGVTALQGLRDKGGIHPGQQVLINGAAGGVG
                     TFAVQIAKTFDTEVTAVCSTRNVEAIRALGADRVVDYTREDFTRDNERYHLVLDVAGS
                     RPWSEAKRVLRPDATVVIVGGPKTNRWLGPLGHVIGMSLASLPASQKSTFFISKADSD
                     DLATLTDLIEGGQVTPVVEQTYPLSRVAEAMSYLGTGHARGKLVITV"
     gene            complement(1994..>2532)
                     /locus_tag="VFU96_08145"
     CDS             complement(1994..>2532)
                     /locus_tag="VFU96_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEU5113278.1"
                     /translation="LDLFRGLNDQGELIPGILVGGEALSHPLNPNGFGSPASVELGLP
                     RTDVDLLGEYPNCAGFVGSFVDDDESVFQTAIEWLALEGITKGCNPPVNDRFCPERTL
                     SRGEMAAFLVRAMGYVDDGGGNRFIDDDASVFESAIDKLATAGVTQGCNPPVNDRFCP
                     DDTVTRGQLAAFLKRALG"
BASE COUNT          477 a          829 c          853 g          373 t
ORIGIN      
        1 ctggtcggcg acaaatgcgg agcgagtcca gaggtcgagg atgcgggcac gtgaggcgcg
       61 gtacccgctc tcgtgctcga tcaccagacc ggtcaactcg acccggccca ccacatcggg
      121 cttgccgggg aacatcgcgg ccacctcgct cggccggggc acgccataga tcccgcacgg
      181 atggtcccat cccggcgcct cggggcagta gtcgcaccat gccacgaagc gctgggtgcg
      241 ccactcggtg accacacctc gcaggaccga gccggtccat tgccaggcac gccaggcgac
      301 caccgcatca ggcgaccagt attcctcggt cggctcgacg gtctggggcg ccgcggtcca
      361 gggcagtggg gtgagggatc gggtgatggc gagcggcgcc aggtcctggg tcggcatctg
      421 gatacggcgg actgtcacca gcaggcccgc ggagaggagg ctgccaccca acagcacgag
      481 gagggcgccg acccgcgggt gcccggtagg gagggcaaca ccctcagttg gcgtcgggcc
      541 catcaccagg aaggcgctgg tgccaccgag tatccagacg accggggtga agtaacggcc
      601 gatccggtct gccgccggct gggagaactt tccgaccagc caggtgatga gacccagggg
      661 aaggaccagc acgatccaga gtggccagaa gccaatggtg tatgcgaaca gggagaacat
      721 ggccacgtcg atccagtaga tcaccgacga ctggccgccg gtgaggacca ccaccagcag
      781 ggggacaccg gccaccgccg ccaggaccag gaccgtgcgg ccaagattcc gcacccaacc
      841 gcgacgaagc agggattggc cgacacctcc agcgacgggt gccggaacgg tcatcctcgt
      901 cccatttctt cgcctccgat gtcaaggata cgggagcgcg cgggggcctg tgcccgaatt
      961 ggctatcgat cgaccgcgat cacgaaagga aatagcctga gccgatgcag gcgatcgtct
     1021 acgaccgcta cggcccgcct gacgtcctcc aacttcgtga agtggcagac ccggaaatcg
     1081 gtgcgaccga cgtcctggtc cgggttcggg cggcgtcggc caaccctctc gactggttcg
     1141 ccttcaacgg gaccccctac atcgcccgcc ccggattcgg gctcaagaaa ccgaactcga
     1201 cgaggctggg ggccgatctg tccggcgtcg ttgcggaggt gggcagtcag gtcacccgtt
     1261 tcgtaccggg cgacgaggtg ttcggggccg gcaccgggtc cttcgccgaa ttggccacag
     1321 tcggcggcac cggagcgatc tcctccaagc ccgccagtct gaccttcgaa caagcggcag
     1381 cggtgccggt cgcgggtgtc accgcactgc aagggctccg ggacaaagga gggatacatc
     1441 ccggccagca ggtgctcatc aatggagcgg cggggggcgt cgggaccttc gcagtccaga
     1501 tcgccaagac tttcgacacc gaggtgaccg cggtgtgcag caccaggaac gtcgaagcga
     1561 ttcgcgccct cggcgcggac agggtcgtcg actacacccg ggaggacttc acgcgcgaca
     1621 acgagcggta ccacctcgtg ctcgatgtgg cagggagccg gccctggtcc gaggcgaaac
     1681 gcgtcctccg accagacgcc accgtcgtga tcgtcggcgg gcccaagacc aaccgctggc
     1741 tggggccgct cggacacgtc atcgggatga gtctggcgtc attgcccgca agccagaaga
     1801 gcaccttctt catatcgaag gcggatagcg atgatctcgc caccctgacc gatctgatcg
     1861 aaggcgggca ggtcaccccg gtagtcgagc agacctatcc attgagccgg gtcgcggagg
     1921 cgatgagcta tctgggcacg ggtcatgccc gggggaagct ggtcatcacc gtctgagggt
     1981 gatcggcccc ggtctatccg agcgcccgct tcaggaacgc ggccaactgg ccgcgggtca
     2041 cggtgtcgtc ggggcagaat cggtcgttga ccggcgggtt gcatccctgg gtgaccccag
     2101 ccgtagccag tttgtcgatc gccgactcga agacactggc gtcgtcgtcg atgaatcggt
     2161 tgccacctcc atcgtcgaca tagcccatcg cccggaccag gaaagcagcc atctccccgc
     2221 ggctcagagt gcgctcgggg cagaaccggt cattcaccgg cgggttgcac cccttcgtga
     2281 tgccttcgag ggcgagccac tcgatggccg tttggaaaac cgactcgtcg tcatcgacga
     2341 acgagcccac gaagccggcg caattcgggt actcgccgag caggtccaca tcagtccggg
     2401 gcagacccag ttcgactgaa gccggcgagc cgaatccgtt cgggttgagg gggtgggaga
     2461 gggcttcccc gcccacaaga atcccaggga tcaactcacc ctggtcgttg agtccgcgga
     2521 acagatcgag cg
//
LOCUS       DASTXO010000390         3489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1165636, whole genome shotgun sequence.
ACCESSION   DASTXO010000390 DASTXO010000000
VERSION     DASTXO010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165636"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..534)
                     /locus_tag="VFU96_08150"
     CDS             complement(22..534)
                     /locus_tag="VFU96_08150"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012832683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEU5113279.1"
                     /translation="MSVRIDGRPELPDGYGVKDDGPFLNWASVEDWLVEANEYWLATT
                     RPDGRPHVVPRWGVWMDERFWYDGSPMTRHARNIASNPSCALHLESGTTVTIIEGTAT
                     FAQPIHGELGQRLAAEYTRKYERLGYAPEADAWSGEDAGGMCVLTPEKGIAWSQFPND
                     LTRFMFTEDQ"
     gene            complement(531..1583)
                     /gene="gcvT"
                     /locus_tag="VFU96_08155"
     CDS             complement(531..1583)
                     /gene="gcvT"
                     /locus_tag="VFU96_08155"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEU5113280.1"
                     /translation="MNRSPLHDLHQRLGARFVDFGGWEMPVQYGSVLAEHRAVRDSAG
                     WFDVTHLGRFELTGSGAERSILSLLSNDMSRIAPGGTQYTLMLNDGGGVVDDLVIWWW
                     EPDRYWVFPNAANHQRVMAAFSSEPGCEVRDFQTETVLIAIQGPEAPSVIEELFGDAP
                     RRFRTASHRWEGGEVGLAGTGYTGERGGEICTDPETGRILAERIISFSVVPCGLASRD
                     TLRLEAGLPLWGSDIDESTTPLEAGLDFAVDMDHDFRGRSALESQMRDGPSRRLIGFV
                     LDDKGIPRHGHKVRTPGGEGEVTSGNLSPLLDTGIGLAYVSPPPVSEAPGIEVSIRDR
                     WVKGHIVQPPFHKASP"
     gene            complement(1580..1930)
                     /locus_tag="VFU96_08160"
     CDS             complement(1580..1930)
                     /locus_tag="VFU96_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113281.1"
                     /translation="MPWDYEPRSFPISWDKDGVPTQFFTPDFYLPDDDLFIEITTMSQ
                     KLVTKKNGKLRRLKELYPEINCKLFYQRDYLNLVVKYGLEGPENLEAAPAPTRMPGRP
                     AVVRLEERGERLIG"
     gene            complement(2014..2484)
                     /locus_tag="VFU96_08165"
     CDS             complement(2014..2484)
                     /locus_tag="VFU96_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU5113282.1"
                     /translation="MTQERELGYRAPQVCKLVGITYRQLDYWARTNLIRPSLQQATGS
                     GSQRLYSFSDVVQLKVIKRLLDAGMSLKKIRSAVTILAEQLDSERPLADVTLLSDGQT
                     IYAAHSEQEVVDVFRRGQGVFGIAIGPVQDEVEGDIHQLFPGEELAGVVEAGAT"
     gene            complement(2590..3114)
                     /locus_tag="VFU96_08170"
     CDS             complement(2590..3114)
                     /locus_tag="VFU96_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003959668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEU5113283.1"
                     /translation="MPGEKEALIAMDVVGVRVELPSNQPIVLLREADGPRYLPIWIGG
                     SEATAIATALEGVVPPRPLTHDLMRSVIDALGALAVRVVITEMRDSVFYADLSLDISG
                     REVHVSSRPSDAIALAVRTGTPVFAFPAVLDDAGVVFEEEEENEEEEVARFREFLEEA
                     TIEDFLGEGQEPPD"
     gene            complement(3151..>3489)
                     /locus_tag="VFU96_08175"
     CDS             complement(3151..>3489)
                     /locus_tag="VFU96_08175"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEU5113284.1"
                     /translation="AMDLSTPVLDRDVIVVEDITDTGLTLSVLRRMIMDRGARSVATA
                     SLIDKKVRRVTEVPIEYRGFEVGDEYLVGYGMDWHGLYRNLRSIWAVLDMEAFVKDPA
                     VLARSLGMTT"
BASE COUNT          628 a         1106 c         1109 g          646 t
ORIGIN      
        1 tgcatgtcga tcgcaccgaa actactggtc ctcggtgaac atgaagcggg tcagatcgtt
       61 cgggaattgc gaccaggcga ttcccttctc cggggtaagc acgcacatcc caccggcgtc
      121 ctcccccgac caggcgtcgg cctccggggc gtaacccagg cgctcatact tccgggtgta
      181 ttccgcggcc agtcgttgac cgagctcgcc gtggatcggc tgggcaaacg tcgcggtgcc
      241 ctcgatgatc gtgaccgtgg tgccactctc gagatggagg gcgcacgatg ggttggaggc
      301 gatgttccgg gcatggcggg tcatcggcga cccgtcgtac cagaaccgct catccatcca
      361 gaccccccac ctgggcacga catgaggccg tccatcgggg cgggtggtgg ccagccagta
      421 ctcgttggct tcgaccaacc agtcctcgac gctggcccag ttcaggaagg ggccatcatc
      481 tttcaccccg tagccgtcag ggagctcggg acggccgtcg atccggacgc tcacggcgat
      541 gccttgtgga agggtggttg gacgatgtgg ccctttaccc agcgatcgcg gatcgatacc
      601 tcgatcccgg gggcctcgga taccggcggt ggcgagacat aggcgagccc gatgccggtg
      661 tcgaggagag ggctcaggtt gccggaagtg acctcacctt cacctccagg agtccgcacc
      721 ttgtggccat gccgcgggat gcccttgtcg tcgaggacaa agccgatcag gcgacgtgag
      781 ggcccgtctc gcatctggct ttccagagcg gaccgtcccc ggaagtcatg gtccatgtcg
      841 acggcgaaat cgagccccgc ttccagcggc gtggtcgact cgtcgatgtc cgatccccac
      901 aacggaagac cggcctcgag acgcaacgtg tcacgagacg ccaggccgca gggcacgaca
      961 gagaaagaga tgatcctctc ggcgaggatt cgaccggttt cagggtcggt acagatctcc
     1021 ccgccacgct cgccggtgta gccggtgccg gccagcccaa cttcgccccc ctcccagcga
     1081 tgggatgcag tgcgaaatcg ccgcggggcg tcaccgaaga gctcttcgat caccgacggt
     1141 gcctccgggc cctggatggc gatcaggacg gtctcggtct ggaagtcgcg cacctcgcac
     1201 ccgggctcac tggagaaggc agccatcacg cgttgatgat tcgccgcgtt ggggaacacc
     1261 cagtatcggt ccggctccca ccaccagatg acgaggtcgt cgacgacccc tccgccgtcg
     1321 ttgagcatga gtgtgtactg cgtcccaccc ggtgcgatcc ggctcatgtc gttgctgagg
     1381 aggctgagga tggaccgctc ggcgccggaa ccggtcagct cgaagcgacc gagatgcgtc
     1441 acgtcgaacc agcccgcgct gtccctcacc gctcgatgct cggcgaggac cgacccatac
     1501 tggaccggca tctcccatcc tccgaagtcg acgaatcggg cacccaatcg ctgatggagg
     1561 tcgtgcaggg gcgaccggtt catccgatga gtcgttcgcc tcgctcctcg agtcgaacga
     1621 cagccgggcg tcccggcatc ctggtgggcg ccggggcggc ttccaggttc tccggccctt
     1681 ccagaccgta cttcaccacc agattgaggt agtcacgctg atagaacagc ttgcagttga
     1741 tctccggata tagctctttg agccggcgga gcttgccgtt cttcttggtg accagcttct
     1801 ggctcatcgt ggtgatctcg atgaagagat cgtcgtcggg gaggtagaag tcgggggtga
     1861 agaattgggt cgggacgccg tccttgtccc aggagattgg aaaggacctc ggctcgtaat
     1921 cccaaggcac gtcgtagaag tcgaggagga gtgcgaattg gcgctcactg ttgtgagcga
     1981 actcggtctc gtgggccgac tttcgctggg tgatcaggtg gctcctgctt cgacgacccc
     2041 ggccaactcc tcaccgggaa acagctggtg gatgtcgccc tccacctcgt cctgcaccgg
     2101 cccgatggcg atgccgaaca ctccctggcc ccgccggaac acgtcgacca cttcctgttc
     2161 ggaatgcgcg gcatagatcg tctggccgtc actgaggagg gtcacatcgg cgaggggacg
     2221 ctccgagtcg agctgctcgg caaggatggt cacggccgac cggatcttct tgagactcat
     2281 ccctgcgtcg aggagcctct tgatgacctt tagttggacc acgtcagaga aagagtagag
     2341 cctctgcgac cctgagccgg ttgcttgctg gagcgagggg cggatcagat tggtgcgcgc
     2401 ccaatagtcc agctgccggt aagtgatgcc gaccagcttg cacacttggg gggcgcggta
     2461 gcccagttcc cgctcttgcg tcatgaaagt cctccgggtt acaaccgtgt aactccgccg
     2521 ttgtgagcaa gctaaccctg tggagggacc cgtgtcaacg aaatctgtgg ataaacctgg
     2581 ggaaagagat cagtcggggg gctcctgacc ctctcccagg aagtcttcga tggtggcttc
     2641 ctccaagaac tccctgaatc tggctacttc ttcctcttcg ttctcttcct cctcctcgaa
     2701 gacgacgcct gcgtcgtcga gaaccgccgg gaaggcgaag acaggtgttc cggtgcgcac
     2761 ggccagagcg atggcgtccg agggtcgcga cgagacgtgc acctcccgcc ccgagatgtc
     2821 gagagagagg tcggcgtaga agacagagtc tctcatctcc gtgatgacga cacggaccgc
     2881 gagcgccccg agcgcatcga tcaccgaccg catcaggtcg tgagtgaggg gtcggggcgg
     2941 gacgacaccc tccagagcgg tggcaatcgc cgtggcctcc gagcctccga tccagatcgg
     3001 gagataacgg ggcccgtcgg cctccctgag gaggacgatc ggctgattgg acggaagctc
     3061 cacccgcacg cccaccacgt ccattgcgat gagggcctct ttttcacccg gcattgccca
     3121 atgaggttat caccgggcag gtcttgccct tcaggtggtc atgcccagcg acctggcaag
     3181 gaccgcaggg tctttgacga acgcctccat gtcgaggacc gcccagatcg agcgaaggtt
     3241 ccggtagagc ccgtgccagt ccatcccgta gccgacgagg tactcgtccc cgacctcgaa
     3301 tccgcggtat tcgatgggga cctcggtcac cctgcggacc ttcttgtcga tgagagaggc
     3361 cgttgcgaca cttcttgcgc cccggtccat gatcatccgt cggaggactg aaagcgtgag
     3421 accggtgtcg gtgatgtcct cgaccacgat cacatctcgg tccagcactg gagtggacag
     3481 gtccatcgc
//
LOCUS       DASTXO010000391         5093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1423734, whole genome shotgun sequence.
ACCESSION   DASTXO010000391 DASTXO010000000
VERSION     DASTXO010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423734"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VFU96_08180"
     CDS             complement(<1..668)
                     /locus_tag="VFU96_08180"
                     /inference="COORDINATES: protein motif:HMM:NF040248.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5113285.1"
                     /translation="MSSWRCGIVPLPTNGSERLTTARATGRGRGRLIGRKVLGSVLTL
                     VFVLVFNFFLFRVVQTDPIGSLFRGRNVPQETLDNLRAAFGLDQSLGTQFLLYLRESA
                     QLNFGISYQTRQPVWNEIVAAIPATVALVGVSAILSAVIGTIGGIIAAWRRNTWKDHS
                     ITSGTMVFYSMPDFWLGMILLVLFAVVLGWFPVGGVSTAGSGESGIALLIDQAYHMFL
                     PALTL"
     gene            complement(619..2373)
                     /locus_tag="VFU96_08185"
     CDS             complement(619..2373)
                     /locus_tag="VFU96_08185"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5113286.1"
                     /translation="MRRLIVSMIAALVAVSLVPIASAQEEDDQVTLRVGLTQDWEVLN
                     PTSGYTVPAYEVWNLHYATLTDKAADDFATTPGLAESWEEVEPGIEYVYTLREGLTWS
                     DGEPLTADDVAWNINTGRDQGWDNMVSTVGNIEATAEDERTVRLVSSVPDPKLPTMDV
                     YLVPQHIWEPIATDYDAATQYAAEDGVGSGPFVMTEFTPGQSVVMAANPNYWGWEGEE
                     PPYDQVIFRLFENPDAMVAALQQGEIDAAQGFPASSWDALEADPNIEVVAGQQGGFDE
                     IAINGGAAEGQPHPALLDVEVRRAIAHGIDRPAVIEDLWFGLASPLETLSPSADPKWI
                     PEIPEENQLAYDPDLANQILDDAGYMDTDGDGVREMPDGTNPLVIRHAVNTDGDLAGS
                     IGDLMVGWMEEIGIGVELEAYDQDALFGVIVEGTYDTFFWGWVPFVDPDPMLSYFTEA
                     EIGNYNDANWFDPAYDALYSEQNQEVDADRRLEIVHEMLTMFHDSAVYIPMYVAPDLQ
                     AYRTDTFEGYVKQPAEIGPVIFSNTSPSYAQLTPIGANAGGSGTNWLLWGGIAAGVIL
                     IVGVFLAMRNRSTSDERE"
     gene            2519..3718
                     /locus_tag="VFU96_08190"
     CDS             2519..3718
                     /locus_tag="VFU96_08190"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEU5113287.1"
                     /translation="MIRETPFHPRTSEANQTGLWSHWSGYLATDRYQMSEKFEYFAIR
                     NSAAVIDTSPLYKYRIAGSDAERFLAGVLARDIRKCRPGQAHYTVWCDDDGWVVEDGV
                     ILRHSDDEFLLTAAEPNMAYLHDLAGRMAVQIEDVSDDFGALALQGPYAREILKQLAP
                     EMEEIGYFHLTPAKVGNAAVTISRTGYTGDLGYEIWVERDDAVDVWDTLFEVAAPYGV
                     IPAGQTALLMARIEAGLVLIDVDFYSARYAWNDEQRTTPHELGLGWMLRDLASDDRAF
                     IGRRAIERELADGTSRWKTVGITVDYEDWNRVYNERGVIPPKDHTPEHGGMMIYDEKL
                     ERIGYTPSFVYSPMVQRHVGIARVRPEHAALGTDVSLEVTIDHRYDVVKAAVSRLPFF
                     NPPRKTA"
     gene            3726..>5093
                     /locus_tag="VFU96_08195"
     CDS             3726..>5093
                     /locus_tag="VFU96_08195"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5113288.1"
                     /translation="MPPKKKFDAIVIGGGHNGLVNGAYLAKAGLSTLILERRHLVGGA
                     AITEELHPGFSFTTFSYALSLLRPEIIHELELTKHGFMPLLMPSTFAPTEDGDYILLG
                     QDKNENHHEISRHSPHDADAYDAYGHMMEQVVHAIRPLMDSIPPDITSKDPEERARMA
                     AIGSYLADLPPGVLHDMIRLMTGSAADILEDYFESDLMQGLHASSGIIGTKVGPRSQG
                     SGLVLLYHSFGEHDGAYGAWAFHKGGNGGFTQVLARSAESFGAEIRLESPVDHVVTKN
                     GRATGVVLEDGTELEAKTVVSAVDPRRTFMQLVDPRELPTDLVDNIRRFKFQGTSAKV
                     NFALDANPTYPGLEGRSDQYRGFINMGPSLDYLERAFDDAKYGWYSQKPYIDGCIQSN
                     VDPDMAPPGKAVMSCFIQYAPYHLKESDWDSERDRFGDVVQKTLEGFFPGFSDLVLHR
                     EVVTPL"
BASE COUNT          975 a         1641 c         1564 g          913 t
ORIGIN      
        1 agcgtcagcg ccggaagaaa catgtgatag gcctgatcga tgagcagcgc gatgccggat
       61 tcgccggatc ccgcagtgga cactccccca acggggaacc accccaggac aaccgcaaac
      121 aggacgagga ggatcatgcc aagccagaaa tctggcatcg agtagaacac catcgtcccc
      181 gaggtgatcg agtgatcttt ccaggtgttc ctccgccagg cggcgatgat gccaccgatg
      241 gtgccgatca cagcggagag gatcgccgag actccaacca gggcaaccgt tgccgggatc
      301 gccgccacga tctcgttcca caccggttgc cgggtctgat aggagatccc gaagttgagc
      361 tgtgccgact ccctcagata gagcaggaac tgggtcccca gcgactgatc gaggccgaaa
      421 gccgcccgca ggttgtccag cgtctcctgc gggacgtttc ttccccggaa caaggatccg
      481 atcggatccg tctgcaccac gcggaagagg aagaagttga acacgaggac gaagacgagg
      541 gtgaggaccg acccgagaac ctttcggcca atgagtctgc cccgcccccg tcccgtcgct
      601 cgagccgtgg tcaggcgctc actcccgttc gtcggaagtg gaacgattcc gcatcgccag
      661 gaagacaccg acgatcagga tcacaccggc agcgatgcca ccccagagca gccagttggt
      721 gccggacccc cctgcattgg cgccaatcgg ggtcagttgg gcatagctcg gtgaggtatt
      781 gctgaagatg accggaccga tctcggccgg ttgcttcacg tagccctcga aggtgtcggt
      841 tcgataggcc tggaggtcag gcgcgacgta catcgggatg tagacggccg aatcgtggaa
      901 catcgtcaac atctcgtgca cgatctccag tctccggtcc gcatcgacct cctggttctg
      961 ttccgaatac aacgcgtcgt acgcgggatc gaaccagttg gcgtcgttgt agttgccgat
     1021 ctcagcctcg gtgaaatacg agagcatcgg gtcggggtcg acaaagggca cccagcccca
     1081 gaagaaggtg tcgtaggtcc cttcgacgat gaccccgaac agagcgtcct ggtcgtaggc
     1141 ctccagctcg actccgatgc cgatctcctc catccatccc accatcaaat cgccgatcga
     1201 tccggccaga tccccatcgg tgttgacggc atggcggatg acgagcggat tggtgccgtc
     1261 gggcatctcc ctgacgccgt caccatcggt gtccatgtac ccggcgtcgt cgaggatctg
     1321 gttggcgagg tcggggtcgt aggcgagctg attctcttcg gggatctccg ggatccactt
     1381 cgggtcggcc gaagggctca gcgtctccag tggacttgcc agaccgaacc agaggtcttc
     1441 gatcacggcg gggcggtcga tgccatgcgc gatggcccgc cgcacttcga cgtcgagcag
     1501 cgccgggtgg ggctggccct cggcggcgcc gccgttgatg gcgatctcgt cgaacccacc
     1561 ctgctggccg gccaccacct cgatgttggg atcggcttcc agcgcgtccc atgagctcgc
     1621 cggaaatcct tgggcggcat cgatctcgcc ctgctggagt gcggccacca tcgcgtcggg
     1681 gttctcgaag agacggaaga tcacctggtc gtacgggggc tcctccccct cccatcccca
     1741 gtagttcggg ttggccgcca tcaccaccga ctgcccgggg gtgaactcgg tcatcacgaa
     1801 aggcccgctg ccaacgccat cctcggcggc gtattgcgtg gctgcgtcat agtcggtggc
     1861 gatcggctcc cagatgtgct gcggcacgag atagacgtcc atggtgggca acttggggtc
     1921 ggggaccgaa gaaaccagcc ggacggttcg ctcgtcttcc gccgtcgcct cgatgttgcc
     1981 caccgtcgaa accatgttgt cccaaccctg atctcgccct gtgttgatgt tccaggcaac
     2041 gtcgtcagcg gtgagtggct ctccatcgga ccaggtcagg ccctctctca aggtgtagac
     2101 gtactcgatg ccaggctcga cctcctccca tgattcggcc agcccggggg tggtggcgaa
     2161 atcgtccgcc gccttgtctg tgagggtggc gtagtggagg ttccagacct cgtacgcggg
     2221 caccgtgtag cccgacgtcg ggttcaggac ctcccagtcc tgggtgagcc cgacacgaag
     2281 ggtgacctgg tcatcctcct cctgggccga tgcgatcgga acaagtgaca ccgcgaccag
     2341 ggcggcgatc atcgacacga ttaggcgtct catcaatttg actctccatt caggttgccc
     2401 catcgggcaa tgtatccgca tcggcgggac tgtgacgccg attcgcctga ccgtgaatcc
     2461 tgaatgacga ttcaggcttc cccggttaca gtgatccgac ccagccgagg ttgcagcatt
     2521 gatccgcgag acaccttttc atcccaggac cagcgaggcc aatcagaccg gattgtggag
     2581 ccactggtcg ggatatctgg ccaccgaccg gtaccagatg tcggaaaagt tcgagtactt
     2641 cgcgattagg aactcggcgg cggtcatcga cacgtccccg ctctacaagt accggatcgc
     2701 cgggtcggac gccgagagat tcctcgccgg cgtcctggcg cgcgacatcc gcaaatgcag
     2761 acccggccag gcgcattaca cggtttggtg cgacgacgac ggttgggtgg tcgaggacgg
     2821 tgtgattctc cgtcacagtg acgacgagtt cttgctcacc gcggccgagc ccaacatggc
     2881 atatctgcat gacctcgccg gtcgcatggc agttcaaatc gaggacgtct ccgatgattt
     2941 cggggccctc gccctgcagg gcccatacgc ccgggagatc ctcaagcaat tggctccgga
     3001 gatggaagag atcgggtatt tccacctcac accggccaaa gtcggcaacg ccgcggtaac
     3061 catctctcgc accgggtaca ccggggatct ggggtacgag atctgggtgg agagggacga
     3121 cgctgtcgac gtctgggata cgctcttcga agtggcggcg ccctacggtg tcatcccggc
     3181 cggccagacg gcattgctca tggcccgcat cgaggccggg ctggtcctga tcgacgtcga
     3241 cttctactcg gcccgatacg cctggaacga cgagcagcga accacacctc atgagctggg
     3301 actggggtgg atgctccgtg atctcgcatc cgacgaccgg gcgttcatcg gccgacgggc
     3361 gatcgaacgt gagctggcgg acggcacatc caggtggaag acagtcggga tcacggtcga
     3421 ctacgaagac tggaaccggg tgtacaacga gcgcggcgtc attcccccca aggatcacac
     3481 ccccgagcac ggcgggatga tgatctacga cgagaaactg gagcggatcg gatacacgcc
     3541 gagcttcgtc tactcaccga tggtgcagcg acacgtcggg atagcgcggg ttcgccccga
     3601 gcatgccgcg ctggggaccg acgtgagcct cgaggtgacc atcgatcacc gttacgacgt
     3661 ggtgaaggcg gctgtgagcc gtcttccctt cttcaacccg ccgagaaaga cagcctgagg
     3721 tagtcatgcc cccgaagaag aagttcgacg ccatcgtcat cggaggcggt cacaacggcc
     3781 tggtcaacgg ggcctacctg gccaaggccg ggttgagcac gctgatcctc gagcgcagac
     3841 atctggtcgg cggtgccgcg atcaccgagg aactgcatcc gggtttcagc ttcaccacct
     3901 tctcgtatgc cctgagcctg ctccgtcccg agatcatcca cgagctggag ctgaccaaac
     3961 acggcttcat gcctctcctc atgccgtcga ccttcgctcc gaccgaagac ggcgattaca
     4021 tcctgctggg ccaggacaag aacgagaacc accacgagat ctcccgccac tcaccccacg
     4081 acgccgatgc ctacgacgcc tacgggcaca tgatggagca agtggtccat gccatccggc
     4141 cgttgatgga ctccataccg cccgacatca cctcgaagga tcccgaggaa cgggctcgca
     4201 tggcagcgat tggctcctat ctcgccgatc ttcctcccgg agtcctccac gacatgatcc
     4261 gcctgatgac gggaagcgcc gccgacatcc tggaggacta cttcgagtcg gacctcatgc
     4321 aaggcctgca tgcctcctcg ggaatcatcg ggaccaaggt cgggccccgg tcccagggct
     4381 cgggtctggt cctgctctat cactccttcg gcgagcacga cggggcctat ggggcctggg
     4441 ccttccacaa gggcggcaac ggcgggttca ctcaggtcct cgcccgatcc gccgagtcgt
     4501 tcggcgccga gattcggctc gagtcgccgg tcgatcacgt cgtgaccaag aatggccggg
     4561 ccaccggggt cgtgctcgaa gacgggaccg agctcgaggc gaagaccgtg gtcagcgcgg
     4621 tcgacccccg ccgcacgttc atgcagctgg tcgatccccg cgagcttccc accgatctgg
     4681 tcgacaacat ccgccgattc aagttccagg ggacctcggc caaggtcaac ttcgccctcg
     4741 atgccaatcc cacctacccg ggtctcgagg gccggtcgga tcaataccgt ggcttcatca
     4801 acatgggccc ttcgctcgac tacctggaac gcgccttcga tgacgccaag tacggctggt
     4861 actcgcagaa gccatatatc gacgggtgca tccaatccaa cgtcgacccc gacatggcgc
     4921 ctcccggcaa ggcggtgatg tcctgtttca tccagtacgc gccatatcac ctgaaggaga
     4981 gcgactggga cagcgagcga gatcggttcg gagacgtcgt gcagaaaacg ctcgaaggat
     5041 tcttccctgg gttcagcgac ctggtgctgc accgggaagt cgtcaccccg ctc
//
LOCUS       DASTXO010000392         3827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1423778, whole genome shotgun sequence.
ACCESSION   DASTXO010000392 DASTXO010000000
VERSION     DASTXO010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423778"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VFU96_08200"
     CDS             <1..450
                     /locus_tag="VFU96_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113289.1"
                     /translation="WVKLPNADLKVDARALEAHPGIENRMGEELVVGVRPNSLEAAAV
                     KGPDPDRTLKAKVEVTEMLGAETYVHFTVNRPPVVTPDIEELLADTGQDVSSLGDTTN
                     FIARVSPDARVSRGDEIDLIVDCAKLHFFDPKSGIRVGSEQKVGASV"
     gene            473..1840
                     /locus_tag="VFU96_08205"
     CDS             473..1840
                     /locus_tag="VFU96_08205"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEU5113290.1"
                     /translation="MSEKAASDAGFPGDFAWGVGTSAYQIEGGRHEQGKGDSIWDRFS
                     DLGRLRDPGDVACDHFHRMREDLDLLADLGVSAYRFSISWPRVIPDGDGRVNQAGLDF
                     YRRLVAGLRARGIAPYVTLYHWDLPQALQDKGGWASRATIDAFSRFACVVAGALGEHV
                     THWITQNEPWVTAMLGHRDGVFAPGVSDWPTAITVGHHLLVSHGAATEEIRGVVPGAK
                     VGIGIDCRPAVPATDSDEDRAATRYFDGTRNRWFFDPVFGRGYPDDVMRVYQDRGRIG
                     QGVIARGDMETIASPIDFLGLNYYTTVAVAAGEEEPAESPPPPGADPEPGHTDLGWKV
                     DPEGLTRYLRHLNDTYAPSSIVITENGASYSDGPDADGVIDDRRRIDYLRSHVGAIAD
                     ARAEQVPVDGYFVWSFMDNLEWVDGFSQRFGLVWVDRETLDRTPKRSFDWYRAVVTSG
                     TFANS"
     gene            1837..2577
                     /locus_tag="VFU96_08210"
     CDS             1837..2577
                     /locus_tag="VFU96_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5113291.1"
                     /translation="MTRTMICHCQAVIAAEDTESLIAPAKAHYDEEHPEYEASLSDLR
                     NYLESEDRSTGPTDRLATIGEVEILPMGPESAMDAIEFFDTDAFPDYPQWGACYCMFF
                     PRGGRSNDNWGQEPWQQNRVDQLHRIEEGRTTGVLAWSNGKVIGWCNATPRAELSGLA
                     TGDDEGIVSIVCFVIAPPYRGHGVATSLLEGVINHFTDGGASRLEAYPVRQANDHRKA
                     FHGTLDLFKRFGFEVVSEEPLVVGLDLR"
     gene            2574..2930
                     /locus_tag="VFU96_08215"
     CDS             2574..2930
                     /locus_tag="VFU96_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011693814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5113292.1"
                     /translation="MSERLDDWMEAYIEAWTTNDDHDIVALFTADAIYDPQTAHGEQH
                     GHEEIVEWWQDVGDHPDNWDFEWLPLIETDDMAVITGTTRYLDPPASYRNLFVIRFDE
                     DGRCTDFTEWYIEEEV"
     gene            2978..3805
                     /locus_tag="VFU96_08220"
     CDS             2978..3805
                     /locus_tag="VFU96_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018653859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU5113293.1"
                     /translation="MSTAFVLGGGGRWGAVEVGMLRALDEAGIEPDLVLGTSIGAFNG
                     SVIADRPGADGVEGLTELWIRIAESDLLQGSRIDRLKNVATLRPALHETSELRALLVS
                     AHARDRRIEDLSIPFQCVAASIERAAEHWFTSGPLIEALLASSAVPALFPPVEIDGEH
                     FYDGGLVNSVPLARAVEMGAQDIYVLQVGRIETPLRPPQRLHEAALISFEIARRHTYH
                     AAVASLPEGIGLHLLPSGNPVAFDDARQLKWRDVSATTELIDGAYTASRAYLEGERS"
BASE COUNT          713 a         1185 c         1245 g          684 t
ORIGIN      
        1 tgggtcaaac ttcccaacgc ggatctgaag gtcgacgccc gggcgctcga ggctcatcca
       61 ggcatcgaga atcggatggg tgaagagctg gtagtggggg tccggcccaa ttctctcgag
      121 gcggccgccg tcaagggccc agacccggat cggacgctga aggcaaaggt cgaggtgacc
      181 gagatgctcg gtgccgagac ctatgtccac ttcaccgtga accgtccccc ggtggtcacc
      241 cccgatatcg aggaactgct ggccgacacc gggcaggacg tttcctcact cggcgacacg
      301 acgaatttca ttgcaagggt gagcccggat gcccgggtct cccgaggtga cgagatcgac
      361 ctgatcgtcg actgcgccaa gctccacttc ttcgacccga agagcgggat ccgggtcggc
      421 tcggagcaga aggtaggcgc cagcgtctga ccagcaggct cccccgggtc gtctgagcga
      481 aaaagcggca tccgacgcgg gcttccccgg cgacttcgcc tggggtgtcg gcacctcggc
      541 ctaccagatc gagggtggtc gccacgagca gggcaagggc gactcgatct gggaccgctt
      601 ctccgacctg gggcggctcc gtgacccagg tgacgtcgcc tgtgaccact tccatcgcat
      661 gcgagaggat ctcgatctac tcgccgatct tggagtctcc gcctaccgat tctcgatctc
      721 gtggccgcgt gtcatcccgg acggcgacgg aagggtgaat caggccggtc tcgacttcta
      781 tcgccgtctc gtcgccgggt tgcgggctcg gggcattgcc ccgtacgtca ctctctatca
      841 ctgggatctg ccacaagctc tgcaggacaa gggtggatgg gcatcgaggg ccacgatcga
      901 tgccttctcc aggtttgcct gtgtcgtcgc aggcgctttg ggcgagcacg tcacccactg
      961 gatcacacag aacgagccct gggtgacggc gatgctgggg caccgtgacg gagttttcgc
     1021 gcccggcgtc tcagactggc ctactgcgat caccgtcggc caccatctac tggtgtcgca
     1081 tggcgctgcc accgaggaaa tccgcggcgt cgtgccgggc gcgaaggtgg ggatcgggat
     1141 cgattgcagg cccgcggtgc ccgccactga ttccgacgag gaccgggccg cgacccgcta
     1201 cttcgacgga acccgcaatc gctggttctt cgacccggtt ttcggccgcg ggtatcccga
     1261 cgacgtgatg cgcgtctacc aggatcgtgg tcgaatcggt cagggagtaa tcgcgagggg
     1321 ggacatggag acaatcgcga gcccgatcga cttcctcggg ctcaactact acacgaccgt
     1381 tgccgttgct gccggtgagg aggagcctgc ggagtcgccc ccaccgcccg gcgccgatcc
     1441 ggaaccgggt cacaccgacc tggggtggaa ggtcgacccc gaaggtctga cccgttatct
     1501 ccgccacttg aacgacacat atgctccgtc ctcgatcgtc atcaccgaga atggggccag
     1561 ctattccgac gggccggatg cggacggagt catcgacgac cgaagacgca tcgactacct
     1621 gagatcacac gtcggggcca tcgccgacgc tcgagccgag caggttccgg tcgacggcta
     1681 tttcgtttgg agcttcatgg acaacctgga atgggtggac gggttctcgc agaggttcgg
     1741 actggtctgg gtggatcgcg agacactcga ccggactccg aagcggagct tcgactggta
     1801 ccgagccgtg gtcacctcag gtacgtttgc caactcatga cgaggacgat gatctgtcat
     1861 tgtcaggcgg tgatcgctgc tgaggacacc gagtcgttga tcgcacccgc caaggcccac
     1921 tacgacgagg agcatcccga gtacgaggcg agtctcagcg acctgaggaa ctatctcgag
     1981 tccgaggatc gctctaccgg ccccacggat aggttggcca cgatcggcga ggtggagatc
     2041 ctgcccatgg gtcccgaatc ggcgatggat gcgatcgagt tcttcgacac cgatgccttc
     2101 cccgactacc cgcaatgggg cgcgtgctac tgcatgtttt tcccccgcgg tggaaggtcg
     2161 aacgacaact ggggccagga gccatggcaa cagaaccgag tggaccaact gcatcggatc
     2221 gaggaaggga gaacgacggg ggtgctcgct tggtcgaatg gcaaggtgat cggttggtgc
     2281 aatgcgaccc ccagggccga gctttccggc ctcgccaccg gtgacgatga gggaatcgtg
     2341 tcgatcgtct gctttgtcat cgcgccgcca tatcgggggc atggagtcgc taccagtctg
     2401 ctcgagggag taatcaacca tttcactgat ggtggagcca gccggctcga ggcgtatccg
     2461 gtccggcagg cgaacgacca cagaaaggcg tttcacggca ccttggacct gttcaaacga
     2521 ttcggcttcg aagtcgtttc cgaggagccg ttggtggttg gcttggatct gagatgagcg
     2581 agcgtctcga cgattggatg gaggcctata tcgaggcctg gacgaccaac gatgaccacg
     2641 acatcgtggc cctcttcacc gccgacgcaa tctatgaccc gcaaacggcc cacggcgaac
     2701 agcacggtca cgaagagatc gtcgagtggt ggcaggacgt cggcgatcac cccgacaact
     2761 gggacttcga atggctgcct ctgatcgaga ccgatgacat ggcggtcatc accggcacca
     2821 cccgttatct ggacccgccg gccagctaca gaaacctctt cgtgatcaga ttcgacgaag
     2881 acgggcgctg caccgacttc accgagtggt atatcgaaga agaggtgtga cagccttgag
     2941 tgccggtagc ccggaccttc cgcttgcgtc ctgagaaatg agcaccgctt ttgttctggg
     3001 gggcggaggc aggtggggag cagtcgaggt cgggatgctg cgtgccctcg atgaggcggg
     3061 gatcgagcct gacctcgtgc tcgggacttc gattggggcc ttcaacggat cggtcatcgc
     3121 cgatcggccg ggcgccgacg gggtcgaggg cctgacggag ctctggatcc gaatcgcgga
     3181 gagcgacctc ctgcaaggga gcaggatcga ccgcctcaag aatgtcgcca cgctgagacc
     3241 tgcgctgcat gagacctcgg agctgcgcgc tttgctggtg agcgcccacg ctcgagatcg
     3301 ccgaatcgag gatctgagca tccctttcca atgcgtcgcc gcatcaatcg aacgagcggc
     3361 cgagcactgg ttcaccagcg gtccgctgat cgaggcgctc ctcgcgtcat cggcggtgcc
     3421 cgctctcttc ccaccggtgg agatcgacgg tgagcacttc tacgacggcg gcctggtcaa
     3481 ctcggtgcca ctggcgcggg ccgtcgaaat gggagcccag gacatatacg tgctccaggt
     3541 gggccgcatc gagacgcccc ttcgaccacc gcagcggctc cacgaggccg ctctgatctc
     3601 cttcgagatc gcacgtcgtc atacctacca cgcagcggtc gccagcctgc ccgaggggat
     3661 cggtcttcac ctccttccct cgggtaaccc ggtcgccttc gatgacgcca gacaactgaa
     3721 atggagagac gtgagcgcga cgaccgagct catcgacggc gcatacacag cgtcgagggc
     3781 ctacctggag ggggaacgct cgtgaaggtg ccgccgttgc cgccacg
//
LOCUS       DASTXO010000393         6213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_797474, whole genome shotgun sequence.
ACCESSION   DASTXO010000393 DASTXO010000000
VERSION     DASTXO010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6213
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797474"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..732
                     /gene="rpiB"
                     /locus_tag="VFU96_08225"
     CDS             88..732
                     /gene="rpiB"
                     /locus_tag="VFU96_08225"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEU5113294.1"
                     /translation="MTAPAGPRSPSQPPPTEQEVRSLVERVLDGVIATDPPSAPEITT
                     SPPTDHALDEGSTTRIAIGADHGGFPLKERIGFRLKEAGYEVLDCGTDSHEAVDYPDF
                     AHAVAREVASGACAYGIVVDGAGIGSAITANKVPGVRAALCYDLSTARNSREHNHANV
                     LTLGAGLIGEALAWQIVEEWLSVEWGGDRHARRVAKIDDIERQYSKAEMSQDHR"
     gene            891..1559
                     /gene="deoC"
                     /locus_tag="VFU96_08230"
     CDS             891..1559
                     /gene="deoC"
                     /locus_tag="VFU96_08230"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008879914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEU5113295.1"
                     /translation="MARYIDHTLLKPEATAAEIDQLCREAEQYRFASVCINPAWVKRA
                     AENLRGTSVAVCTVVAFPLGATTPEVKATEARRALRDGAREVDMVLNIGALKSGDYDL
                     VQTDIEKVVDAAHDSGALCKVILETALLTDEEKVIASSLAKKARADFVKTSTGFGPGG
                     ATVYDVALMRETVGPDMGVKASGGVRTTGDAEDMIAAGATRIGASAGIEIVGGKDGGS
                     GERY"
     gene            1543..2178
                     /locus_tag="VFU96_08235"
     CDS             1543..2178
                     /locus_tag="VFU96_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017720546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113296.1"
                     /translation="MAKGIELRSFAFLDSLQPQYAAFLGTVAQGFLPLAGDASLWIEV
                     APGIEINRLTDVALKATSVKPGLQIIERYYGVLEVHSPDQSDTRAAGAAILEGMESSE
                     DDRIKPRILSSQIIRRLDDHHAQLINRTRHGQMIIPGQTLYVLECEPAAYASLAANEA
                     EKAAEINVLEVRSFGSMGRVYLGGEEQDIDVGWQAAVAALEGLSGRDAGQG"
     gene            2227..2529
                     /locus_tag="VFU96_08240"
     CDS             2227..2529
                     /locus_tag="VFU96_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HEU5113297.1"
                     /translation="MAEALGMIEARSFPAMVEAADAMVKAAKVELVSMEKTGGGYVTA
                     VVRGDVAAVKAAVDAGVRGAEKVGEVIATHVIARPHVNIDLVLPLGRQDQAQDDLG"
     gene            2566..2877
                     /locus_tag="VFU96_08245"
     CDS             2566..2877
                     /locus_tag="VFU96_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEU5113298.1"
                     /translation="MHLGRVAGTLVASRKEPLMEGMKLLIVRQLDADNAETGSYVVAV
                     DAVGAGEGEIVLYASGSSARQTDLTRDRPVDAVVMAIVDSWDVGGEERYHKGREEGRT
                     R"
     gene            2880..4337
                     /locus_tag="VFU96_08250"
     CDS             2880..4337
                     /locus_tag="VFU96_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5113299.1"
                     /translation="MLSDAEIQAIIERVRGRVAAAEISGRAAPALLAADELAVSEIEL
                     GDGIHADIDAAVTAARTAFGDYREMGLEKRKTIVEAMRASMLREGERLGYMAAEETGL
                     GRGEDKAIKNRLVTTRTPGPEDLEPQVVTGDSGMMVTEYAPYGVIGSITPTTNPTSTI
                     INNAIAIVSAGNAVTFNVHPNARMVSVENVQLLNRAIVGAGGPPDLVTAIPSPTLESA
                     KELMNHPEVRVLLVTGGPGVVKEALKTSKKAITAGPGNPPAVVDQTADIDAAGRAIVR
                     GASFDNNVICTDEKTTIVVDTVADRLVQAMVAAGAYRLKEHELKRLERVIFREMGPPG
                     KPGLINTEWIGKDASKILAEIGVTTGEDTRLLVAEVPRDHNLVWTEQMMPVMPVVRVS
                     NVDDAIDLAVRSEHGFRHTASIHSTNVETITRMARAMNCSIFVANGSNFNGLGEGGEG
                     FTSFSIASPTGEGLTRPRTFSRVRRVTVVGALRIV"
     gene            4330..4935
                     /locus_tag="VFU96_08255"
     CDS             4330..4935
                     /locus_tag="VFU96_08255"
                     /inference="COORDINATES: protein motif:HMM:NF013129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HEU5113300.1"
                     /translation="MFERDSAIALLEFESISAGIVAGDAIVKTAPLGAIYAGTVHPGK
                     YLVLVTGDTASVDEAVRVGKDSESLVDMVFLPDVHPAVASAIAGNASRAPVGDEALGV
                     IETTTAAAAIDAADAGAKAADTDLAAIRLADGLGGKGYVLFSGVVAEVEAALAAAVER
                     VGDHGTLVRSDLIAQLHEEMAENLRAELRFLSRVSAPTRGG"
     gene            4937..5212
                     /locus_tag="VFU96_08260"
     CDS             4937..5212
                     /locus_tag="VFU96_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEU5113301.1"
                     /translation="MQLGRVVGSVVATEKAPGLEGIRFLILQPLDKRSRPQGETLVAA
                     DAVSMAGPGELVYFVASREAAEALPQQFVPVDHAIVGIVDDVHLEEE"
     gene            5213..5500
                     /locus_tag="VFU96_08265"
     CDS             5213..5500
                     /locus_tag="VFU96_08265"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEU5113302.1"
                     /translation="MKVGRVAGTVISTINIPLLEEHRLLLCDVHDVDGVPSGYTIAVD
                     VVDAGVGDTVLILDEGSSARQILGLDRGAIRAVVVGIVDQLVVDGQVRAIP"
     gene            complement(5497..>6213)
                     /locus_tag="VFU96_08270"
     CDS             complement(5497..>6213)
                     /locus_tag="VFU96_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113303.1"
                     /translation="SDDFGQIVLMSEERLTAAQATADRIDRWVWGIVILTVILIITTM
                     FVSQTRRRTGIQLGIGIAVAFVVSAAVLNRVESALVAEIANPVAGETASALFGHLLSG
                     LRNGIIAVIVISTVIALVLYLIGRPQWVTTTGEKVGTLMPGSLETGGMNAWVAARYDL
                     LRLVAAAIAVLALFLIGFGIVTLLVIGALLALVLWWLAQARDRAVAQPEVVAVSESQQ
                     IEAVSDSEQTDAVTDPESGV"
BASE COUNT         1125 a         1951 c         2147 g          990 t
ORIGIN      
        1 cgcatcataa gaacatcgaa cgccggggtc cttggcgtct ggccatcttc ctaggatggg
       61 ctcggtgagg aggccgtagc caacgcgatg acagcacccg ccgggccccg gtctccttcg
      121 caaccgcctc ccaccgagca agaggttcga tccctcgtcg aacgtgtcct cgacggggtc
      181 atcgccactg atccgccgtc tgcccccgag atcacgacca gcccaccgac cgaccacgct
      241 ctcgacgaag gctccacgac caggatcgcc atcggggccg accatggtgg gttccccctc
      301 aaggaaagga tcgggttccg gctcaaggag gccggatacg aggtgctgga ctgcggaacc
      361 gatagtcacg aggccgtcga ctaccccgat ttcgcccacg ccgttgcccg cgaggttgcc
      421 tcgggcgcct gcgcttacgg aatagtcgtc gacggggccg ggatcgggtc ggcgattacc
      481 gccaacaagg tgcccggagt acgcgcagcc ctctgttacg acctgtccac tgcccggaac
      541 agccgggagc acaatcacgc caacgtcctc acactcggag ccggattgat cggcgaggcc
      601 ctggcctggc agatagtcga ggagtggctg tcggtcgagt ggggtggcga tcgccacgcc
      661 cgtcgggttg ccaagatcga tgacatcgaa cggcaatact ccaaagccga gatgagtcag
      721 gatcaccgat gaatcgggag gcgctcgtcg aggcggtcac ccgcgaggtg ttggcagccc
      781 ttgccctgcg cgacgacgta tgcgacaccg gggagaaggt ccgcgaggtc gtcgccaatg
      841 gggccgatcg agtcgcattc tacggggagg cggccgaggt ccccctggac atggcccgct
      901 acatcgacca caccctgctg aagccggagg caaccgcggc cgagatcgac caactctgtc
      961 gtgaggccga gcaatatcgc ttcgcgtcgg tgtgcatcaa cccggcctgg gtgaagcgcg
     1021 ccgcggagaa cctgagaggc acatcggtcg cggtgtgcac cgtcgtcgca ttcccgctcg
     1081 gcgccaccac tcccgaggtg aaggcgacgg aggcgcgtcg ggcgctgcgt gatggggccc
     1141 gggaagtgga catggtgctc aacatcggcg ccctcaagtc gggtgattac gacctggtgc
     1201 agaccgacat cgagaaggtc gtcgatgctg cccacgacag cggggcgttg tgcaaggtca
     1261 tactggagac ggcgctgctg acggacgagg aaaaggtcat cgcctcctca ctggccaaga
     1321 aggccagggc ggatttcgtg aagacctcga cgggcttcgg tccgggaggg gccacggtct
     1381 acgacgtggc gctgatgcga gagaccgtag gccccgacat gggggtcaag gcgtcgggag
     1441 gggtgcgcac caccggggat gcagaggaca tgattgccgc cggggcgacc agaatcgggg
     1501 cgtcggccgg gatcgagatc gtcggaggga aggacggagg cagtggcgaa aggtattgag
     1561 ctgaggtcgt ttgcgttcct ggacagtctc cagccgcaat acgccgcctt tctggggacg
     1621 gtggcccagg gctttctccc tctcgccggc gacgcctccc tctggatcga ggtagccccc
     1681 gggatcgaga tcaaccggct gacagacgtg gccctcaagg cgacctcggt caagccaggc
     1741 ctccagatca tcgagcgtta ctacggcgtc ctcgaggtgc actctcccga ccagtcggac
     1801 acgcgggcgg ccggagcggc catcctcgag gggatggaga gcagtgagga cgatcggatc
     1861 aagccgcgca tcctctcgtc gcagatcatc cgcaggctcg acgaccatca cgcccagctc
     1921 atcaaccgga cccggcatgg tcagatgatc atcccgggcc agaccctgta cgtgctcgag
     1981 tgcgagccgg ccgcctacgc ctccctcgcc gccaacgagg cggagaaggc ggcggagatc
     2041 aatgtcctcg aggtgcgcag cttcggctcg atgggtcggg tgtacctcgg tggtgaggag
     2101 caggacatcg atgtcggatg gcaggcggcg gtggcggccc tggagggtct ctccggaagg
     2161 gacgccggcc aaggttgatg caacgggatg agcctatgag tcgaacacaa ggaaaggagg
     2221 agcttcatgg ctgaagctct gggaatgatc gaggcacgca gcttcccggc gatggtggag
     2281 gcggcagatg ccatggtcaa ggcggccaag gtcgaattgg tcagcatgga gaagacgggt
     2341 ggcggatacg tcacagcagt ggttcgtggt gatgtcgcag ccgtcaaggc ggctgtcgac
     2401 gccggagttc gcggtgccga gaaggtgggc gaggtcatcg ccacccacgt catcgcccgg
     2461 ccccatgtca acatcgattt ggtgctcccg ttgggtcgcc aggaccaggc gcaggacgac
     2521 ctcggctagc cgagcccacc ttcgaaccgg gcaggaggtc tcaccttgca tctaggccgc
     2581 gtcgccggga ctctggtcgc cagcaggaag gagcccctca tggaggggat gaagctgctg
     2641 atcgtccgcc aactcgacgc ggacaacgcc gagaccggca gctacgtggt ggctgtggat
     2701 gcggttgggg caggcgaggg agagatcgtc ctgtacgcct cgggtagttc ggcgcgccag
     2761 accgatttga cccgcgaccg tccagtcgat gccgtcgtga tggcgatcgt ggattcgtgg
     2821 gacgtgggtg gcgaggagcg gtatcacaag ggccgggagg agggtcgcac ccgatagcga
     2881 tgctgagtga cgcagagatt caggccatca tcgagcgggt caggggccgc gtagccgccg
     2941 ccgagatctc cggtcgggcc gcgccggcgc tcctggctgc ggacgagctc gccgtctcgg
     3001 agatcgagct cggcgatggg atccacgccg acatcgacgc tgccgtgacc gccgcccgga
     3061 ccgcgttcgg cgactatcgg gagatggggc tggagaagcg aaagacgatc gtcgaggcga
     3121 tgcgcgcgtc gatgctgcgg gagggggagc ggctcggcta catggcggcc gaggagacag
     3181 gcctgggacg tggggaggac aaggcgatca agaaccgcct ggtgacgacc aggacgccag
     3241 ggcccgagga tctcgagccg caggtagtga ccggggactc agggatgatg gtcaccgagt
     3301 acgcccccta cggcgtcatc gggtcgatca ctcccactac gaatccgacg tcgaccatca
     3361 tcaacaacgc cattgccatc gtctcggcgg gcaacgccgt caccttcaac gtccacccca
     3421 atgcccggat ggtctccgtg gagaacgtcc agctcctcaa cagggccatc gtcggcgccg
     3481 gtggacctcc cgacctggtg accgccatcc cctccccgac cctcgagtcg gccaaggagt
     3541 tgatgaatca ccccgaggtc agggtcttgc tcgtcaccgg tggccccggt gtggtcaagg
     3601 aggcgttgaa gaccagcaag aaggcaatca ccgccggacc gggcaaccca cctgcggtcg
     3661 tcgatcagac cgcagacatc gatgctgccg gtcgggccat cgtgcggggc gcatcgttcg
     3721 acaacaacgt gatctgcacc gacgagaaga ccacgatcgt ggtcgacacc gtggcggatc
     3781 gtctcgtcca ggcgatggtg gcggccgggg cgtaccggct caaggagcat gagctgaagc
     3841 gcctggagcg ggtcatcttc agggagatgg gcccgccggg gaagccgggg ctgatcaaca
     3901 cggagtggat cggcaaggat gcgagcaaga tcctggccga gatcggtgtg acgaccggag
     3961 aggacacgcg tcttctcgtc gccgaggtcc cccgggacca caacctcgtc tggaccgagc
     4021 agatgatgcc ggtgatgcca gtggtccggg tgagcaacgt ggacgatgcc atcgacctgg
     4081 cggtccgatc cgagcacgga ttccgccata cggcctcgat ccacagcacc aatgtcgaga
     4141 ccatcaccag gatggctcgg gccatgaact gcagcatctt cgtggccaac ggctccaact
     4201 tcaacggcct gggtgaaggt ggggagggat tcacgtcttt ctcgatcgcc agcccgaccg
     4261 gggagggatt gacccgcccc cgcaccttct cccgggtgcg gcgagtgacc gtggtcgggg
     4321 ccttgcgcat tgtttgagcg cgactctgcc atcgccctcc tcgagttcga gtcgatcagt
     4381 gccggcatcg ttgctggtga cgcgatcgtg aagaccgccc cgttgggcgc catctacgcc
     4441 ggcacggtgc atccgggcaa gtatctggtg ctggtgaccg gcgacacggc cagcgtcgat
     4501 gaggcggtgc gggtgggcaa ggactccgag tccctcgtcg acatggtgtt cctccccgat
     4561 gtccatcctg ccgtcgcctc tgccatcgcc gggaacgcat cccgggcccc ggttggcgac
     4621 gaggcattgg gtgtcatcga gaccaccacc gcggcggccg caatcgatgc agccgacgcg
     4681 ggcgcaaagg cggccgacac agatctcgcc gcgattcgcc tcgccgacgg gctggggggc
     4741 aagggctacg tccttttctc gggtgtggtg gccgaggtcg aggcggccct ggccgccgcc
     4801 gtcgaacggg tcggggacca cggcaccctt gtccgcagcg acctcatcgc ccagctccac
     4861 gaggagatgg ccgagaacct ccgtgccgag ctccgattcc tgagccgcgt gtccgccccc
     4921 accaggggag gctgagatgc agctcggtcg tgtcgtgggg agtgtcgtgg cgaccgagaa
     4981 agcgcctggt ttggagggga ttcgctttct catactgcag ccgctcgaca agaggagccg
     5041 gccccagggt gagactctgg tcgctgccga cgcggtctcc atggccgggc ccggcgagct
     5101 cgtctacttc gtcgcctctc gagaggccgc ggaagctctg ccccagcagt tcgtccccgt
     5161 cgaccatgcc atcgtcggga tcgtcgacga tgtccatctc gaggaggaat gaatgaaggt
     5221 tggacgagtc gcggggaccg tgatctcgac gatcaacatc ccgttgctcg aagagcaccg
     5281 gctcctgctc tgcgacgtgc atgacgtcga cggggtccct tcgggctaca ccatcgccgt
     5341 ggacgtggtc gacgccggag tgggcgacac cgtgctgatc ctcgacgagg gaagctccgc
     5401 ccgccagata ttgggcctcg accgtggcgc gatccgcgcc gtcgtagtcg gcattgtcga
     5461 ccagctcgtg gtggacggcc aggtccgggc gatcccttag acgccggact ccgggtcggt
     5521 gacggcgtcc gtctgctcgg agtcgctgac cgcctcgatc tgctgcgatt cgctgaccgc
     5581 aacgacctcg ggctgtgcga ctgcccgatc ccgcgcctgt gccagccacc aaaggacgag
     5641 tgcgagcagc gcgccgataa cgagcagggt gacgattcca aacccgatga ggaagagcgc
     5701 gagcacggcg attgcggccg cgacgagtct gagcaagtcg taacgagccg cgacccaagc
     5761 gttcatgccg ccggtctcga gactgcccgg catcaatgtg ccgaccttct cgccggtggt
     5821 tgtgacccac tggggtcggc cgatcaggta gagcaccagg gcgatgaccg tcgagatcac
     5881 gatcacggcg attatcccat tgcggagccc ggagaggagg tgcccgaaga gggcgctggc
     5941 ggtctctccc gccaccggat tggcgatttc ggcgaccagc gcggactcga cacggttgag
     6001 cactgctgca gacacgacga aagccactgc gatcccgatc ccgagttgga tgccggtgcg
     6061 tcgacgggtt tgcgacacga acatcgtcgt gatgatcagg atgacagtga ggatcacgat
     6121 cccccacacc cagcggtcga tccggtcggc agtcgcctga gcggcggtca gccgttcctc
     6181 ggacatcagg acgatctggc cgaagtcatc gga
//
LOCUS       DASTXO010000394         7328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_908012, whole genome shotgun sequence.
ACCESSION   DASTXO010000394 DASTXO010000000
VERSION     DASTXO010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7328
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908012"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1039
                     /gene="sucD"
                     /locus_tag="VFU96_08275"
     CDS             116..1039
                     /gene="sucD"
                     /locus_tag="VFU96_08275"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEU5113304.1"
                     /translation="MSILVGKDTRVLIQGLGKTGQFHTEKAIAYGTQMVGAVHPSRKG
                     SVAHFEGETDHSGVPGRDDEYSVDLPVFATVEEAAAATGANASVVYVPPPFAADAIME
                     AGAAGLDLVVAITEGVPVADMIKAKRYLADKATRLIGPNCPGVITPGECKIGIMPGYI
                     HERGRVGVISRSGTLTYEAVYQLTLNGLGQTTAVGIGGDPVNGTNFVDMLRMFEDDPE
                     TDGVIMIGEIGGTAEEDAAEFVAASMNKPVAGFIAGTTAPPGKRMGHAGAIVSGGKGT
                     AAAKIEAMTAAGIVMAETPSDMGEAMARALG"
     gene            1026..1688
                     /locus_tag="VFU96_08280"
     CDS             1026..1688
                     /locus_tag="VFU96_08280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEU5113305.1"
                     /translation="MLSAENLAQIRAGGDRVLELARRDRDRPVPQYPDWVMVDLAAHL
                     GSIHARTTQICRELPAERPSAPPPPEHTDVLDWCEDNLAEMLSALGGADPDSRAWGFW
                     PESTLGLWERRMVIETGVHRWDADQAFGDPGPLTDLVALSGLNEFEAMWLPRLGELPV
                     LEVKAVDLGRSWMFASGSPLRTVSGTASDLYLRLMSRPSKVELPVQWAAAVDALEPPP
                     KR"
     gene            complement(1690..2400)
                     /locus_tag="VFU96_08285"
     CDS             complement(1690..2400)
                     /locus_tag="VFU96_08285"
                     /inference="COORDINATES: protein motif:HMM:NF014635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HEU5113306.1"
                     /translation="MNRADQSDALTDLLGLEHPPIAITFHGDLPDDGVPRFDEPMSEP
                     TEDDRSGRVPAPCVFWMHAHEKTFDTMAEDHGNCSVGRFTHGFAEAADILNMNDVAAL
                     LDVGWVTMEAFSGVATLARRPEGVRYGPLAAANALPDVVLLRVTPRQMMEINDAVEVD
                     WSGKPQCQILPKAANEGVIAASMGCALSRARTGMGDDELTVAVPGDRVEELVAALRPV
                     TTADNAVVGYAVADLARF"
     gene            complement(2431..2814)
                     /locus_tag="VFU96_08290"
     CDS             complement(2431..2814)
                     /locus_tag="VFU96_08290"
                     /inference="COORDINATES: protein motif:HMM:NF042760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU5113307.1"
                     /translation="MTSEGRFGAAITCMDGRIQTRTIDQVMTRFGVRYVDNITATGAV
                     QHLDGSVTPTGEGLLQSLTVSEQAHGTNQVAVVAHAGCAGNPVPDAQQKEQLRRARAV
                     VAERFPAMEVIALFFDPRIGFEKVS"
     gene            2876..3430
                     /locus_tag="VFU96_08295"
     CDS             2876..3430
                     /locus_tag="VFU96_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013980979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU5113308.1"
                     /translation="MDLIWIVVIAVLVILVFAFIGIYNGLVQSRNRVDNSWAQVDVQL
                     KRRYDLIPNLVETVKGYASHERETFEQVTRARSAAQAASGPAEQAQAEGFLTQALRQL
                     FAVAEAYPELRATENFQNLQNELAETENKIAVSRQIYNDTVLTYNNKVQQIPTNIVAS
                     MTGFQTREFFEAGPEAVDAPTVDF"
     gene            3513..4067
                     /locus_tag="VFU96_08300"
                     /pseudo
     CDS             3513..4067
                     /locus_tag="VFU96_08300"
                     /inference="COORDINATES: protein motif:HMM:NF021489.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            4293..5294
                     /locus_tag="VFU96_08305"
     CDS             4293..5294
                     /locus_tag="VFU96_08305"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEU5113309.1"
                     /translation="MDYDREYEQEPPTDLKPAEVGALLTQGAVSEKEFTATMFDLIRQ
                     GAIKATPSQIERSTWGGLRDETITDLVLELTDKDTGYRDYEQSVLSVVGRVLDEGPRP
                     LHEFREAIREDASENASSYQSFRGKVSSAIKRAGLLDERGNGVAWFVAIAVGLLVFGA
                     IVFLPNILERRPGGQTMAIFIVIGMVLGALALYIFLMFRRIRVRRSPTGALEAERWDA
                     FRRYLTDFSRLKEAPPISLELWDKFLIYAIVFGVAEEVLQQARLHAPPELEQTSSIYW
                     FGTYGYSGGHTENAFAGLNSALTGAFSPPASSGGGGGFSGGGGGGGGGGGGGGGGAW"
     gene            5342..6163
                     /locus_tag="VFU96_08310"
     CDS             5342..6163
                     /locus_tag="VFU96_08310"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEU5113310.1"
                     /translation="MRSLFAVVVFVVGTSTAVPAQAAEEEFPVYTGDEFKDLYDYAVA
                     KVLPNLMPPSGRLPISGDPVLDGRIWDIATARGYVLRPVAGPELGSADGVPMQPQAAA
                     AWLELKRAARAAGNEFVVSSAYRSPESQRTQFISKLRGTTDEAIDAALTWYSIPGTSK
                     HHSGYALDFRYADGSFGEFRDTPDYAWLAADNFYNAKRFGFIPSYPDDVTAQGPNPEP
                     WEFVWVGVDLVECGMPCVDAMTGPRARHPGGVTPVDFGLDRNARRKHWRRVPVIG"
     gene            complement(6196..6639)
                     /locus_tag="VFU96_08315"
     CDS             complement(6196..6639)
                     /locus_tag="VFU96_08315"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEU5113311.1"
                     /translation="MRDGLLFLHIVGVAGWLGGGVYAMYTYSALATMEPSAAAAALQR
                     LKKVEDRFFGPASGLVLLSGIGLVLTSEAFGWGDAFVLIGLGAFLISAILGATIGKRN
                     SERLIEAANTGSGVEDALRSWRAGTVWDFVILAVVIWAMITKLGA"
     gene            complement(6649..>7328)
                     /locus_tag="VFU96_08320"
     CDS             complement(6649..>7328)
                     /locus_tag="VFU96_08320"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5113312.1"
                     /translation="QNDRAGYVDLLVEEMLPAAAPLADYCDVFVEEGAFTVDEARHIF
                     AAAEKAGMRPRVHADQLSHSGGAALAAEIGAVSADHLDHVTEEDATALAAAGVVGVLV
                     PGASYMMRSPQAPGPMLIRQGVTVAIASDCNPGTSYLESMGLVISLAVVQMGLTTDQA
                     IHAATRGGALALGMEGQGTIAPGSAGDLVVLDAPSPEHIPYRPATSLVWKTIKAGRVV
                     AGDRAPG"
BASE COUNT         1268 a         2354 c         2455 g         1251 t
ORIGIN      
        1 ggctcgaagg aaccaatgtc gaggagggca aacggctcct gaacgagtcg gggctcgacc
       61 tggtcgctgc cgacagcctg aaggacggcg ccgagaagat cgtggggctg gcccgatgag
      121 cattctcgtc ggcaaggaca cccgggtcct catccaaggg ctgggaaaga ccggccagtt
      181 ccataccgaa aaggcgatcg cctacgggac ccagatggtg ggggcggtac atccctcccg
      241 aaaggggagt gtggctcatt tcgagggtga gaccgaccat tccggtgtac cgggtcgtga
      301 tgacgagtac tcggtggacc taccggtgtt tgccacggtg gaggaggcgg cagccgcgac
      361 gggggccaat gcctcggtgg tctatgtgcc gccgccgttt gccgccgacg ccatcatgga
      421 ggcaggcgcc gccggcctcg atctcgtggt tgccatcacc gagggtgtcc ctgttgccga
      481 catgatcaag gccaagcgct atctcgccga caaggcgacc cgtctcatcg ggcccaattg
      541 ccccggtgtg atcacgccgg gagagtgcaa gatcgggatc atgcccggtt acatacacga
      601 gcggggcagg gtgggtgtca tctctcgatc aggaaccctg acctacgaag cggtgtacca
      661 gttgacgctc aacgggctgg gccagacgac agcggtcggg atcggcggag accccgtgaa
      721 cgggaccaac ttcgtggaca tgctccgcat gttcgaggat gacccggaaa ccgacggtgt
      781 gatcatgatc ggggagatcg ggggcactgc tgaggaagat gcagccgagt tcgtcgccgc
      841 ttcgatgaac aagccagtgg ccgggttcat cgccggtacc acagccccac ccgggaaacg
      901 gatggggcac gccggcgcca tcgtctccgg aggcaagggc acggccgccg ccaagatcga
      961 ggcgatgacc gcggccggca tcgtgatggc cgagaccccc agtgacatgg gcgaggccat
     1021 ggcgcgtgct ctcggctgag aacctggccc agatccgggc cggcggggac cgtgtcctgg
     1081 agctggctcg gcgggatcgg gatcgaccgg ttccgcagta ccccgactgg gtcatggtcg
     1141 atttggcagc acatctgggc tcgatccacg ctcggaccac gcagatttgt cgggagctcc
     1201 ccgctgagcg cccctccgct cccccgccac ctgagcacac cgatgtcctc gactggtgcg
     1261 aggacaacct cgcggagatg ctctcggccc tcgggggggc cgacccggac agtcgggctt
     1321 gggggttctg gccggagtcg acgctcggcc tctgggagcg gaggatggtc atcgagaccg
     1381 gggtccaccg gtgggacgct gatcaggcgt tcggggatcc gggcccgttg accgacctgg
     1441 tggctctttc ggggctgaac gaattcgagg cgatgtggct gccacgtctt ggcgaactgc
     1501 cggtgctcga agtcaaggcc gtggacctgg ggcggagctg gatgttcgcc tcgggatcgc
     1561 cattacgaac tgtgagtggg acggcgtccg acctctacct ccgcctcatg tcgcggccgt
     1621 ctaaggtcga gctacccgtt cagtgggcgg cggccgtcga cgctctcgag ccgccgccga
     1681 agcgatgaat cagaaccggg cgagatcggc gactgcgtag cccactaccg cgttgtcggc
     1741 ggtggtcacc ggtcgcaggg cagcaaccaa ctcctcgact cgatcaccgg gcacggccac
     1801 cgtgagctcg tcgtcgccca tcccggtgcg ggcccgggag agagcacagc ccatcgatgc
     1861 cgcgatcaca ccttcgttcg ctgccttggg aaggatctga cactgaggct tgcccgacca
     1921 gtcgacctcc accgcgtcat tgatctccat catctgacgg ggggtgacgc ggaggagcac
     1981 aacgtcgggg agggcgttgg ccgcggccag cgggccatac ctcacaccct cagggcggcg
     2041 ggcgagcgtc gcgacacccg agaaagcctc catcgtcacc caaccgacgt cgagcagggc
     2101 cgctacgtcg ttcatgttga ggatgtcggc ggcttcggcg aagccatggg tgaatcggcc
     2161 caccgagcag ttgccgtggt cttcggccat cgtgtcgaag gtcttttcgt gagcatgcat
     2221 ccagaagacg cagggggcgg gcacgcgacc cgagcgatcg tcttcggtcg gctccgacat
     2281 cggctcgtcg aaccggggga cgccgtcatc gggcaggtcg ccatggaaag tgattgcgat
     2341 agggggatgc tccaggccga ggagatcggt cagtgcatcg gattggtcgg ctcgattcat
     2401 catttcctcg ttcggggtcc ggaacggaac ctagctcacc ttctcgaatc cgattctcgg
     2461 gtcgaagaag agggcgatca cctccatcgc cgggaaccgc tccgccacga ccgccctggc
     2521 tcttcgcagt tgctccttct gctgggcgtc ggggacgggg tttccggcgc acccggcgtg
     2581 ggcaacgacg gcgacctgat tggtgccgtg agcctgctct gacacagtca ggctctgcag
     2641 aaggccctct ccggtgggcg tcaccgatcc gtccagatgc tgcacggccc cggtggcggt
     2701 gatgttgtcc acgtagcgga ctccgaaacg ggtcatcacc tggtcgatgg tccgggtctg
     2761 aatcctccca tccatgcaag tgatggctgc gccgaaccgg ccctccgagg tcatcgcgtc
     2821 attctggcct tccgggaggc aggccgcagg cggcgtggat ctacactgcc cgcccatgga
     2881 tctcatctgg atcgtcgtga tcgccgttct ggtgatcttg gtgttcgcct tcatcggcat
     2941 ctacaacggg ctcgtccagt cacgaaacag ggtcgacaac tcctgggcgc aggtcgacgt
     3001 acagctcaag cgtcgctacg acctgatccc caacctggtg gagacggtga agggctacgc
     3061 ctcccacgag cgggagacat tcgagcaggt cacacgggcg aggagcgcgg cgcaggccgc
     3121 cagcggcccg gcagagcagg cccaggccga ggggttcctc acccaggcgc tgcgtcaatt
     3181 gttcgccgtg gccgaggcct acccggagct gcgggccacc gagaacttcc agaacctgca
     3241 gaacgagctt gcagagaccg agaacaagat cgcggtctcc cgtcagatct acaacgacac
     3301 cgtcctcacc tacaacaaca aggtgcagca gatccccacc aacatcgtgg cgtcgatgac
     3361 cgggttccag acccgtgagt tcttcgaagc cgggcccgaa gcggtggacg ccccgaccgt
     3421 cgatttctga cttgggccgg ctcacccgag ctctgatcgt cgtcctgctc gtcgtcgctc
     3481 cggcaacgac ggccctggcc aaatcgttct ggatgagcga cgcagacgtc gaaatcgtcg
     3541 tcaacgacga cggctccctg tcgatcaccg agagcctcac ctatgacttc gacggggatt
     3601 tctccggggc ctaccgggac atcccccttc gtccgggcga ggccatcacc ggcgtcaccg
     3661 tgagcgacga gtcgatcgtg tacgagccgg gcggctgtac cgaactgggc tgttcctcga
     3721 gcccgggcac ttatggagtc gaagaacagg gcgacctggt acgcatcgtg tggcatcact
     3781 cgtcctcgaa cgaacagagg acatttcaga tcagctagga gatgacgggt gttgccgttg
     3841 catacgacga cggaggtgat gtgaacctgc aggtctgggg tgaccagtgg gcggtgggtc
     3901 tggactcgct cgaagccagg acgaacctgc ccgccggcgc cgaacccggt gaagtcctgg
     3961 tctacggcca cccctacggt gtcgacggcg agacgtcgtt ggggcccgat gaggtatccc
     4021 catccttgga ggccagcaat gttccggcct atcagtgggt ggagctgcgg actgtcttcc
     4081 ctcggtcact gctcgcttcc accgacggag ccactgtcgt ccccggcaac gggctggagg
     4141 cgatcctcgc cgaggaagag gcgttcgccg aggaggcctc ggatgcgaca actgcagctc
     4201 gaacgggcct gatctggggg tcggtcctcg ccgtagctct gtccctcggg gctggcagcc
     4261 tggtctactt ccgatatggc agagaaccga aggtggacta cgaccgggag tacgagcagg
     4321 agcctccaac cgacctgaag ccggccgagg tcggggcgct cctcacccag ggggcggtca
     4381 gcgagaagga gttcaccgcc accatgttcg atctgatccg gcaaggggcg atcaaggcga
     4441 caccgtccca gatcgagcga agcacctggg gtggcctgcg tgacgagacg atcaccgacc
     4501 tcgttctcga gctgaccgac aaggacaccg ggtaccgcga ctacgagcag tccgtgctgt
     4561 ccgtcgtcgg acgggtcctc gacgagggac cccgtcccct ccatgagttc cgggaggcga
     4621 tccgcgaaga cgcctccgag aacgcgtcga gctatcagag cttccggggc aaggtgtcct
     4681 cggcgatcaa gcgagccggc ctcctcgacg agagggggaa tggtgtcgcc tggttcgtgg
     4741 cgatagcagt ggggctgctc gtgttcgggg cgatcgtgtt cctgcccaac atcctcgagc
     4801 gccgacccgg cgggcagacg atggccatct tcatcgtcat cggcatggtg ctcggggcac
     4861 tcgccctcta catattcctc atgttccgtc ggattcgggt gcggcgatct ccgacgggag
     4921 ccctggaggc ggagcggtgg gatgcgttcc ggcgttatct caccgacttc tcacggctga
     4981 aggaggcgcc cccgatctca ctggagctgt gggacaagtt cctgatctac gcgatcgtgt
     5041 tcggagtggc ggaggaagtg ctgcagcagg cccgcctcca cgccccaccc gagctggagc
     5101 agacatcctc gatctattgg ttcggcactt atgggtactc ggggggccac accgagaacg
     5161 cctttgccgg cctcaactcc gccctcaccg gcgccttttc ccctccggca tcgagcggtg
     5221 gcggcggggg cttctccggc ggaggtggtg gcggcggagg tggtggtggc ggaggtggtg
     5281 gcggcgcctg gtagcgcggt cgacgagcaa acgtcgcgcc cggtttagcg tgtccacaag
     5341 gatgagatcc ctctttgccg tcgtcgtctt tgtcgtgggg acatcgaccg ctgtccccgc
     5401 gcaagcggcg gaggaggaat tccccgtcta caccggtgac gagttcaagg acctgtacga
     5461 ctacgcggtg gccaaggtcc ttcccaatct gatgcctcca tcgggtcgcc tccccatcag
     5521 cggggacccc gtgctcgacg ggaggatctg ggacatcgcc acggcccgtg gttatgtgct
     5581 gcggccggtc gccggcccgg agctggggag tgccgatggc gtgccgatgc agcctcaggc
     5641 cgccgccgcc tggctggagc tgaagagagc cgccagggcg gccgggaacg aattcgtcgt
     5701 ctcgtcggca tatcgatccc ccgaaagcca gcgaacgcag ttcatttcca agctgagggg
     5761 caccaccgac gaggcgatcg acgccgccct cacgtggtac tcgatcccgg gcacctcgaa
     5821 gcaccacagc ggatacgcac tcgactttcg atatgccgat gggtcgttcg gcgagttcag
     5881 ggacacaccg gattacgcct ggctggctgc ggacaacttc tacaacgcca agcggttcgg
     5941 gttcatcccc tcgtaccccg acgacgtcac cgcccagggt cccaaccccg agccttggga
     6001 gttcgtgtgg gtcggggtgg acctggtcga gtgcgggatg ccctgcgtcg acgccatgac
     6061 cggcccgagg gcgcgtcatc caggcggggt gacaccagtc gatttcgggc tcgatcgaaa
     6121 cgcccggcgc aagcattggc gccgggtccc cgtcatcggg tgagcagata gaaggccgag
     6181 aacttgttcc cgcgctcagg cccccaactt ggtgatcatc gcccagatga ccaccgcgag
     6241 tatcacgaag tcccacacgg tcccggctcg ccacgatctg agcgcgtcct ccacaccgga
     6301 tccggtgttc gccgcctcga tgagacgctc gctgttccgc tttccgatcg ttgcgccgag
     6361 gatcgcgctg atcaggaagg cgcccaaccc gatgaggaca aaggcatcac cccatccgaa
     6421 ggcctccgag gtgaggacga gccctatccc cgagagcagg acgagaccgc tcgccgggcc
     6481 gaagaaccgg tcctccacct tcttcagacg ctgcagcgcc gccgctgcag ccgagggctc
     6541 catcgttgcc agcgccgagt atgtgtacat ggcgtagacg ccgcccccga gccagccggc
     6601 cactcccacg atatggagga acaagaggcc atctcgcatg gatcgaccct acccgggtgc
     6661 tcgatcgccg gccacgaccc ggccggcctt gatcgtcttc cacaccaggc tggttgccgg
     6721 gcggtagggg atgtgctccg gggacggggc atcgagcact accagatccc cggccgagcc
     6781 gggtgcgata gtgccctgtc cctccatgcc aagggcgagg gctccgcccc tcgttgcggc
     6841 atggatcgcc tgatcggtcg tgaggcccat ctggacaact gcgaggctga tcaccaggcc
     6901 catcgactcc agataggaag tcccggggtt gcagtcgctg gcgatggcga cggtgacgcc
     6961 ctgtcggatc agcatcggcc ctggtgcctg gggtgagcgc atcatgtagg aggcaccggg
     7021 gacgaggact ccaaccacgc ctgctgctgc cagggctgtc gcgtcttcct cggtgacgtg
     7081 gtcgagatga tcggcgctga ccgctccgat ctcggcagcc agcgccgccc caccggaatg
     7141 agacagttga tcggcgtgga cgcggggcct catgcctgcc ttctcggcgg ccgcgaagat
     7201 gtgtcgcgcc tcatcgaccg tgaaggcgcc ttcctcgacg aagacgtcgc aatagtccgc
     7261 caaaggggcg gcggcgggca gcatctcctc gacgaggagg tcgacatagc cggcccggtc
     7321 gttttgga
//
LOCUS       DASTXO010000395         2796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_24050, whole genome shotgun sequence.
ACCESSION   DASTXO010000395 DASTXO010000000
VERSION     DASTXO010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24050"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="VFU96_08325"
     CDS             complement(<1..896)
                     /locus_tag="VFU96_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5113313.1"
                     /translation="MKKGRQRSALAALLAFGLFLAACSSGEPTETTEAGGEPTTTEGT
                     TDTTAAPTTETTEAPAAEGGIITTYIGEPEFLQPPNTNESEGNAVLSALFRGLIDYNP
                     QTSEPQFTGVAESITSPDEGLTWEIVLKDGWTFHDGTPVTAQSYVDSWNYSAYGPNAA
                     QVSGFFASIAGYEDLQCGTVTEPDPETGEDVDVADCEGQPPAAEELSGLSVTDDLNFT
                     VTLAAPETFFMTRLGYTAYYPLPEAFFADPAAYNEAPIGNGPFMMDGTWEHDVEIRTV
                     ANPDFAGEDQAQIDGLTFRIYA"
     gene            complement(1008..1778)
                     /locus_tag="VFU96_08330"
     CDS             complement(1008..1778)
                     /locus_tag="VFU96_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018663168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5113314.1"
                     /translation="MKIEGSVALVTGGVSGLGLGAASRFVRGGGSVVMIDIDEAHGAA
                     ALATLGGNARFVHCDVRDPQDVAEAVRRAAEEWGRIDILVNAAGVAPAQRLISRDGEL
                     FDLDLFKFVIEVNLVGMFDMTRNVAAVMSRNDPGPDGERGVIVNVASIAAFEGQIGQA
                     AYSASKGGIVGMTLPLARDLAGIGVRCVTVAPGIMDTPLLAGAPEELKESLAALHVFP
                     RRLGTPEDFAHLVQAIVENQLLNGEVIRLDAGARMPPR"
     gene            1844..2623
                     /gene="murI"
                     /locus_tag="VFU96_08335"
     CDS             1844..2623
                     /gene="murI"
                     /locus_tag="VFU96_08335"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEU5113315.1"
                     /translation="MIGVFDSGVGGLSVLREIRREMPFADLLYLADQGRAPYGVRTLT
                     EVADLTDQAARWLLDRGATTITIACNTASAAALHALRASHPDVPIVGMEPAVKPAAMS
                     TASGVIGVVATAATFQGELFASVMQRHAAGASVLTRACPSWVELVERAEVDGPEAMSQ
                     VESCLAPILDGGADTLVLGCTHFPFLIPLIERAAGPELTIVDPAPAVARQVRRVATAT
                     KGSGRLTLATSGDPEEFRRVARALAGIEALEPVLACRWNDS"
     gene            2613..>2796
                     /locus_tag="VFU96_08340"
     CDS             2613..>2796
                     /locus_tag="VFU96_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113316.1"
                     /translation="MTAEAFELAVGDALSRAPDSAWIHRDMALFGWGVSARIDVGTGA
                     DRFDRAMRRLSSFDAPL"
BASE COUNT          480 a          939 c          894 g          483 t
ORIGIN      
        1 tcggcataga tccggaaggt caggccatcg atctgggcct ggtcctcacc ggcgaagtcc
       61 gggttggcca ccgtcctgat ctccacgtcg tgctcccagg tgccgtccat catgaacgga
      121 ccgttaccga tcggcgcctc gttgtacgca gccggatcgg cgaagaacgc ctccgggagc
      181 gggtagtacg cggtgtagcc gagccgggtc atgaagaacg tctcgggcgc ggccagggtg
      241 acggtgaagt tgaggtcgtc ggtgaccgac agaccactca gctcctccgc tgcggggggc
      301 tggccctcgc agtcggccac gtcgacatcc tcaccggtct ccggatcggg ctccgtcacg
      361 gtcccgcact gcaggtcttc gtagccggcg atcgaagcga agaaacccga cacctgggcc
      421 gcattgggcc cgtatgccga gtagttccac gaatcgacgt aggactgcgc cgtgaccgga
      481 gtgccgtcgt ggaaggtcca tccgtccttg aggacgatct cccaggtgag accctcgtcg
      541 ggagaggtga tcgactcggc gacaccggtg aactggggct cactggtctg cgggttgtaa
      601 tcgatcaggc cgcggaacag ggcgctgagc accgcgttcc cttccgactc gttggtgttc
      661 gggggctgca agaactccgg ctcaccgata taggtcgtga tgatcccacc ttcggccgcg
      721 ggggcctcgg tggtctcggt agtaggcgcg gcagtggtgt ccgtagttcc ctcggtggtg
      781 gtcggttctc cgcccgcctc cgtggtttca gttggttccc cgctgctaca cgccgccagg
      841 aacagtccaa acgcaagaag agcggcaagc gcgctcctct gtcgtccttt cttcatatga
      901 gttccctcct ttgggcagac gggctgttac gcccatcaag cggggagcat atacctccac
      961 cacgagcaac acatagtgac aacgtatggt tttttggtac cgaacgatca cctgggaggc
     1021 attcgcgccc cggcatcgag acggatcacc tcaccgttga gcagttgatt ctccacaatc
     1081 gcttgcacca gatgggcgaa gtcctcaggg gttccgagac gacgagggaa aacatgaagc
     1141 gcggccaggg attccttgag ctcttcgggg gcaccggcga ggaggggagt atccatgatc
     1201 cccggtgcca cggtgacgca tctgacgccg atcccagcca gatcccgggc gagcggcagc
     1261 gtcattccga cgattccccc tttcgatgcg gagtacgcag cctgcccgat ctgaccctcg
     1321 aacgcagcga tcgatgccac gttgacgatc accccccgct cgccatccgg gcccggatcg
     1381 ttacgggaca tcaccgcggc gacattccgg gtcatatcga acatgccgac gagattcacc
     1441 tcgatgacga acttgaacag atccagatcg aaaagctcac cgtcgcgcga gatcaagcgc
     1501 tgagccggcg caaccccagc ggcgttgacc aggatgtcga tccgtcccca ctcctctgcg
     1561 gctcttcgca ctgcctcggc tacgtcctgg ggatcacgca cgtcacaatg gacgaacctg
     1621 gcgttccctc ccagtgtggc cagcgccgcg gcgccgtggg cctcgtcgat atcgatcatg
     1681 accactgatc ccccgccccg gacgaaacgg gaagcggcac ccaaaccaag cccggacacg
     1741 ccacccgtga ccagcgccac cgagccctcg atcttcacgc aggacctcct ctgggcggcg
     1801 acgctaccac cgggcccctg atcgccctgc tgagatggca ccgatgatcg gggtcttcga
     1861 ctctggggtc ggcggcttga gcgtgttgcg cgagatccga cgggagatgc cattcgccga
     1921 cctgctctat ctcgccgacc agggacgggc tccatacggg gtgcgaacac tgacggaagt
     1981 cgccgacctg acagaccagg ccgccagatg gctcctcgac cgcggggcca ccacgatcac
     2041 tatcgcctgc aacacggcct cggccgccgc gcttcatgcg ctacgggcca gccaccccga
     2101 tgtcccaatc gtgggaatgg agccggcggt gaagccggcg gccatgagca cggccagcgg
     2161 cgtcatcgga gtggtggcga cggccgcgac cttccaaggc gagctcttcg catccgtgat
     2221 gcagcgtcac gccgcaggag ccagcgtgct gacccgggcc tgcccgagct gggtcgagct
     2281 ggtcgagaga gccgaagtcg acgggcccga ggcgatgagt caggtggagt cctgccttgc
     2341 ccccatcctc gatggcggcg cggacacctt ggtgctgggt tgcacccatt tcccgttcct
     2401 gatccctctc atcgagaggg cggcggggcc cgagctgacg atcgtcgacc cggcgccggc
     2461 ggtcgcccga caagtgcgga gagtcgcgac tgccacgaag ggatcgggcc ggctgaccct
     2521 ggccacttcc ggcgacccag aggagttccg ccgcgtcgca cgcgccttgg ccgggatcga
     2581 ggccctcgag cccgttctag cctgcaggtg gaatgacagc tgaagccttc gagctggccg
     2641 tgggcgatgc cctctcccgc gcccccgatt cggcttggat ccaccgcgac atggccctct
     2701 ttggctgggg cgtctcggcc aggatcgacg tcggcaccgg agccgatcga ttcgaccggg
     2761 ccatgcgacg gttgtcgagc ttcgatgccc cgctgg
//
LOCUS       DASTXO010000396         4078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1313427, whole genome shotgun sequence.
ACCESSION   DASTXO010000396 DASTXO010000000
VERSION     DASTXO010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313427"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="VFU96_08345"
     CDS             complement(<1..958)
                     /locus_tag="VFU96_08345"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014143681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU5113317.1"
                     /translation="MSLVVQKFGGTSLADAARIRAVADRVESTRSEGHDVVVVVSAMG
                     ETTDQLVALAAEVSEDPSPREMDMLLSAGERISMALLSISLNSRGIPAVSYTGSQAGI
                     LTDSSHGSAKILDIRGTRVRDSLAQGRVVIVAGFQGLDPESKEIPTLGRGGSDATAVA
                     LAAALGADSVEIYTDVDGVFTADPRMVPDAMKLDEIPFDEMLELSAGGAGVLMSRAVE
                     FGRNFDIPIHVRSSFHDGDGTWVRESPMEQAIVSGIAHDRSEAKVTVNRVPDRPGVAA
                     ALFGSLATAGVNIDMIVQNVSHDGSTDISFTVPRANLVDAERI"
     gene            1043..1831
                     /locus_tag="VFU96_08350"
     CDS             1043..1831
                     /locus_tag="VFU96_08350"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU5113318.1"
                     /translation="MTDRRRRQKEQRAAKREAEKKQEARKELGRRLGTALVFGVVVVG
                     IVVAGAYFGSDEGELPVGYEGFRDQPTACGAEKPDPEPVLSFPAPEPQTDVTPESTVT
                     VTLETSCGDIGIELDPAASPVTANSFVFLVREGFFDGQVFHRIVEDFRIFSGDPEANG
                     SGGPGYRIADEFPPDDLVLAPGMVMMDNVGSGTTGSQFFILTGDAASALNPQFNLLGE
                     VVSGQDTLEAIAAVETAVRPGSTEQSLPLETVYIERATVDVTGT"
     gene            1867..2706
                     /locus_tag="VFU96_08355"
     CDS             1867..2706
                     /locus_tag="VFU96_08355"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEU5113319.1"
                     /translation="MAVDLHTHSTASDGSESPSALVDMAAGLGLSVLALTDHDTLEGI
                     GEARDSAARNGVDLISGTEISLDYLGGMHLLVLWLEPGPGPLQDRLGGLQDGRVGRNA
                     AIVEALTDLGMPMSIEEVIAQSGGGSVGRPHIAAVMMRKGYVDSVAEAFDLWLGTGRP
                     AYAARPRLSPEEAIGLARESGGVPILAHPHTLGINRSHEMADMLVRLRSAGLVGLEAM
                     YAAYHRHEREGYADLARRFGLVASGGSDYHGSYKPGLELGIGYGDLVVPDRVVDSLRD
                     AVS"
     gene            2703..>4078
                     /gene="murJ"
                     /locus_tag="VFU96_08360"
     CDS             2703..>4078
                     /gene="murJ"
                     /locus_tag="VFU96_08360"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEU5113320.1"
                     /translation="MSRPLKMGAAAAVISASILISRLLGLGREALLATLIGVNTNGDL
                     YRQAFLIPDFLNYLLAGAYLTITLIPILSQHLEQDDPEGASRAFTSVFRFVGWAILGL
                     TVAMWLAAEPIVSLIFPRVPEPERLVSLTRLVLPAQVFLVMGALLMAVQYTHRRFLLP
                     ALAPIIYNLGIIIGGLVGAAIGEPSPEAFLWGAVIGSAVGNFGLQWFGARSTGTWLTR
                     VPPHGSSVKEYLVLALPLMLGQSVAVLDEQFVRLFGQLDVGATAALSFARQLNMVPVG
                     VIAQAAGVAAFPFLARLAAGGDLDGLSRTTGRAARNTVFVAAAAMAALIVLARPLVSL
                     LYEHGEFSAADGSLVARLLVLYALSIPAWGLHQILSRHFYAQRMMWRVVLIGTAGTLI
                     AIPVWLGLYSAMGVEGFALASTLVMTAYALALLVAWGFDAGWSPVRALIPSILRGLVA
                     AGLAAAVA"
BASE COUNT          635 a         1364 c         1306 g          773 t
ORIGIN      
        1 caatgcgctc ggcgtcgaca aggttggcgc ggggcacggt gaagctgatg tctgtgctgc
       61 cgtcatgtga gacgttctgc acgatcatgt cgatgttgac ccccgccgtg gccagggacc
      121 cgaacaaggc cgcggcgaca cccggacggt cgggcacccg gttcaccgtc acctttgcct
      181 cggatcggtc gtgggcgatg ccgctcacga tggcttgttc cataggggac tcgcgcaccc
      241 acgtcccgtc gccatcgtga aacgaggacc tgacgtgaat cggtatgtcg aagttgcgcc
      301 cgaactcgac cgctctcgac atgaggacac cggcgccgcc ggcggagagc tcgagcatct
      361 catcgaacgg gatctcgtcg agtttcattg catcgggcac catccgcgga tcagcggtga
      421 acaccccgtc gacatcggtg tagatctcga cgctgtccgc ccccaaagcc gcggctaggg
      481 cgacggcggt ggcgtcggac cccccgcgac ccagcgtcgg tatctccttc gactcgggat
      541 ccagtccctg gaacccggcc acgatcacca cccgcccctg ggccaggctg tcccgaaccc
      601 ttgtccctcg aatatcgagg atcttggccg acccgtggga ggagtcggtg aggatgcctg
      661 cctgagaacc ggtgtagctc accgccggaa tgccacgaga gttgaggctg atcgagagca
      721 aggccatcga gatccgctcc ccggcgctca acagcatgtc catctcgcgg ggcgatgggt
      781 cttcgctcac ctcggcggcc agggccacca gttgatcggt ggtctcgccc atggcggaga
      841 caaccaccac gacatcgtga ccctcactcc gcgtggactc cacgcggtcg gccactgccc
      901 tgatccgggc tgcgtccgcc aaggaagttc cgccgaattt ctggacgacc agagacatga
      961 ccaacagttt ggaacgtttc ccggccaaac acgaacaccg acctcgggat tgcacagggc
     1021 tcgtgcctag agtcgctccg ccatgaccga tagacgcagg cgtcagaagg agcagcgcgc
     1081 cgccaagaga gaggccgaga agaaacagga agcccgtaag gagctggggc gaaggctcgg
     1141 gactgcgctc gtgttcggtg tggtggtggt cgggatcgtc gtcgccggtg cctacttcgg
     1201 aagtgacgag ggcgagctcc ccgttggcta tgaggggttt cgcgaccaac caacggcgtg
     1261 cggggccgaa aaacccgatc cagagccggt tctctccttt cccgcccccg agccacagac
     1321 cgacgtcacc cctgagtcca cggtgacggt cacgctggag acctcatgtg gtgacatcgg
     1381 catcgagctc gatccggcag cctcccccgt gacggccaat tcctttgtgt tcctggttcg
     1441 tgaggggttc ttcgacggcc aggtgttcca ccgcatcgtc gaggatttca ggatcttctc
     1501 tggcgacccc gaggcaaatg gaagtggcgg tcctggttac cggatcgccg acgagttccc
     1561 tcccgacgat ctcgtgctgg cccccggcat ggtgatgatg gacaacgtgg gcagcggaac
     1621 cacggggagc cagttcttca tcctcaccgg ggacgcggcc tcggcgctca atccccagtt
     1681 caatctcctc ggcgaggttg tgtctgggca ggacactctc gaggcgatcg ccgcggtcga
     1741 gactgcggtc cggcccggat cgaccgagca gagccttccg ctggagaccg tctacatcga
     1801 gcgagccacg gtggacgtaa ccggaacttg agggcccgcc gggccatcat ccctgtctga
     1861 gaatcaatgg ccgtcgacct ccacacccat tccacggcgt ccgacgggtc ggagtccccg
     1921 tccgccctcg tcgacatggc ggctggcctg gggctgagtg tgctggccct gaccgatcac
     1981 gacacgctcg aaggcatcgg ggaagcgcgc gattcggcag ccagaaacgg agttgacctc
     2041 atctccggga ctgagatctc actcgattac ctcggcggga tgcacctttt ggtcctctgg
     2101 ttggaaccgg gcccggggcc gctccaggac cggcttggag ggcttcagga cgggcgtgtc
     2161 gggcggaacg ccgccatcgt cgaagccctg accgatctgg ggatgcccat gtctatcgag
     2221 gaggtgatcg cacaatcagg gggtggctcg gtgggacgac cccatatcgc cgcagtgatg
     2281 atgcggaagg gttatgtcga ctcggtcgcc gaggccttcg atctgtggtt gggaacggga
     2341 cggccggcct acgccgcaag gccgcgtctc tcccccgaag aggcgatcgg tctcgctcgc
     2401 gagtcgggcg gcgtgccaat tctggcccac ccccacaccc tggggatcaa tcgctctcac
     2461 gagatggccg acatgctggt acggctccgc tcggcgggtc tggtcggttt ggaggcgatg
     2521 tatgccgcct atcaccgcca tgagcgtgag gggtatgccg acctcgcccg gcgcttcggg
     2581 ctcgtcgcat ccggaggcag cgattaccac ggttcgtaca agccgggcct cgagctcggc
     2641 atcggctacg gggatctagt ggtccccgac cgcgtcgtcg attcactccg ggacgcggtg
     2701 tcatgagccg gccgctcaag atgggtgctg cggcagcagt gatctcggcc agcatcctca
     2761 tctcccgtct cctcggcctc ggccgcgagg cgctcctcgc cacgctcatc ggggtcaaca
     2821 ccaacggcga cctctaccgc caggcctttc tcatccccga cttcctcaac tatctgttgg
     2881 ccggggccta tctcaccatc actctcatcc ccatcctgag ccagcacctg gaacaagacg
     2941 accctgaggg ggcaagccgc gcgttcacct ctgtgttccg gttcgtcgga tgggccatcc
     3001 tggggctcac agtcgccatg tggctggctg cagagccgat cgtctctctg atcttccccc
     3061 gggttccaga acccgagcga ttggtgtccc tgacccggct ggttctcccc gcccaggtgt
     3121 tcctggtgat gggcgccttg ctcatggccg ttcaatacac gcatcgtcga tttctgttgc
     3181 ctgccctggc cccgatcatc tacaacctcg gcatcatcat cggtgggctt gtcggggcgg
     3241 cgatcggcga gccgagcccg gaggcatttc tctggggcgc ggtcatcggc tcggcggttg
     3301 gcaacttcgg actgcagtgg tttggggcga ggtccactgg cacatggctc accagggtcc
     3361 cgccccacgg ttcgtcggtg aaggagtacc tggtgttggc gcttcccctg atgctcggcc
     3421 agtcggtggc cgtcctcgac gagcagttcg tcaggctgtt tggccagctc gacgtgggtg
     3481 ccaccgccgc cctcagtttt gcccgccaac tgaacatggt cccggtcggg gtgatcgccc
     3541 aagcggcagg ggtggctgcc ttcccgttcc tggcccgtct cgccgcggga ggcgatctcg
     3601 atgggttgag ccgaacaacc ggtcgtgcgg cccggaacac ggtcttcgtc gccgcggccg
     3661 cgatggcagc gctgatcgtt ctcgctcgtc ccctcgtcag cctgctctat gagcacggcg
     3721 agttctccgc cgctgatggc agcctcgtcg cccgtctcct tgtcctctac gcactctcca
     3781 tccccgcctg gggtctccac cagatcctct cccgccactt ctatgcccaa aggatgatgt
     3841 ggcgggtggt gctgatcggc accgcgggca ccctcatcgc catcccggtt tggctcggcc
     3901 tctactcggc gatgggcgtc gaggggtttg ccctggcctc cacgctggtg atgaccgcat
     3961 acgccctggc cttgctcgtc gcttgggggt tcgacgccgg gtggtcgccg gtgcgagccc
     4021 tgattccgtc gattctccgg ggcctggtcg ctgccgggct cgcggcggcg gtggccta
//
LOCUS       DASTXO010000397         2474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_134711, whole genome shotgun sequence.
ACCESSION   DASTXO010000397 DASTXO010000000
VERSION     DASTXO010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134711"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /gene="ribD"
                     /locus_tag="VFU96_08365"
     CDS             complement(<1..441)
                     /gene="ribD"
                     /locus_tag="VFU96_08365"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEU5113321.1"
                     /translation="MRRAIELAAPHRTHPNPRVGAILVDESGDVVGEGAHLGAGLPHA
                     EMVALQEAQDRARGSTLYVTLDPCSHQGLTPPCSPAVVEAGVRRVVIGAIDPDPRVSG
                     AGVSWLREAGIEVETGVLEDEAGALDPAYFHHRRTGLPLVTLKTA"
     gene            complement(474..1403)
                     /locus_tag="VFU96_08370"
     CDS             complement(474..1403)
                     /locus_tag="VFU96_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5113322.1"
                     /translation="MSERVLIADDDPDILAVVQVNFELEGFEVETAVDGEDALQKATA
                     TPPHVIVLDIMMPRMDGLTALHRLRSQASTGNIPIILLTARGLPEDRVRGLELGADDY
                     ITKPFDINELVARVRAVVRRTQAARDLSPLTGLPGNFKITGEVEERISSGRDFALVHG
                     DLDNFKAFNDHYGFMRGDEVIRFCAKCFNDAADSLGIAGAFVGHIGGDDFVAIIPPEV
                     SESFCKEVIERFDDGILDLYDTADALRGYIEVIDRRGERFAFPVVSLSLGVASTDVRE
                     ISTQWEASAIAVEMKEFAKKQQGSTYRIDRRTT"
     gene            complement(1400..2062)
                     /gene="rpe"
                     /locus_tag="VFU96_08375"
     CDS             complement(1400..2062)
                     /gene="rpe"
                     /locus_tag="VFU96_08375"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU5113323.1"
                     /translation="MAALKIAPSLLAADFTCLENQILAVEKEADWLHIDVMDGHFVPN
                     ISFGIPVIAGIRPMTTLRFDCHLMTTNPDAYLTDLAEAGADSVTMHIEAMPDPTDAAK
                     QAREIGLGFGLVCNPATPFAAVQPFMELSDLILIMSVEPGFGGQGFMPEVLTKVEEAR
                     EWVEDHGLRTDIQIDGGINLETASQAVAAGANVLVAGTTIFGSDDPPTAAAELRRAIE
                     GK"
     gene            complement(2095..>2474)
                     /locus_tag="VFU96_08380"
     CDS             complement(2095..>2474)
                     /locus_tag="VFU96_08380"
                     /inference="COORDINATES: protein motif:HMM:NF014908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113324.1"
                     /translation="PSYLDGTREPVPQSDEGLTYAPKIEPQERVIRIGGTRSEEVNRV
                     RALSPEPGATLVIDGERHQVVQARARETGPETGRWMEVEGVPVAGLGDGGMELVTLLP
                     AGKRAMSGASWLRGLRRSSGTIA"
BASE COUNT          414 a          802 c          796 g          462 t
ORIGIN      
        1 cgcggtcttc agagtgacca gaggaagccc cgtccgacga tggtggaaat aggcagggtc
       61 cagtgcccct gcctcgtctt ccagcacccc ggtctcgact tcgatcccgg cctctctcag
      121 ccaggaaacg cctgccccgg acactcgggg atctgggtcg atcgccccga tcacgactct
      181 ccgcacaccg gcctcgacca cagcaggaga acagggaggg gtgagaccct ggtgactgca
      241 tggatcgagt gtcacgtaga gggtggaccc tctcgctcgg tcctgtgctt cctggagtgc
      301 gaccatctcg gcgtggggca ggcctgctcc gaggtgggcg ccctctccga cgacgtcacc
      361 cgattcgtcc accaagatgg cgccaaccct cgggttggga tgagtccggt ggggtgcggc
      421 gagctcgatc gctcgtcgca tgtgacgggc atcgcccact cagcggcccg ccactaagtc
      481 gtgcgtcgat cgatgcggta ggtggagccc tgctgcttct tggcgaattc cttcatttcc
      541 accgcaatcg ccgacgcctc ccactgtgtg gagatctcgc gcacgtcggt ggacgctaca
      601 cccagggaca gggaaaccac agggaacgcg aaccgctccc cgcgccggtc gatcacctcg
      661 atgtacccgc ggagagcatc ggcggtgtcg tagaggtcga ggatcccatc atcgaagcgc
      721 tcgatgacct ctttgcagaa ggactcggag acctcgggcg ggatgatcgc gacgaagtcg
      781 tcgcccccga tgtgcccgac aaaggcaccg gcgataccca ggctgtcggc ggcgtcgttg
      841 aagcacttcg cacagaagcg gatcacttcg tcgccacgca tgaacccgta gtggtcgttg
      901 aaggccttga agttgtcgag gtcgccatgg acgagggcaa agtcgcgacc ggaggagatc
      961 cgctcttcga cctccccggt gatcttgaag ttgccgggca gaccggtgag tggcgagagg
     1021 tcgcgggcgg cctgcgtcct gcggaccacg gcacgaaccc gggcgacgag ctcgttgatg
     1081 tcgaaaggct tggtgatgta gtcgtcggcg cccagctcca ggccacgcac gcgatcctcg
     1141 gggaggccgc gggcggtgag gaggatgatc gggatgttcc cggtcgaggc ttggctgcgc
     1201 aaccgatgga gggcggtgag cccgtccatt cgaggcatca tgatgtcgag gacgatcacg
     1261 tgaggagggg tggcagtggc cttctgcagc gcatcctcgc cgtcgactgc ggtctccact
     1321 tcgaacccct cgagctcgaa gttgacctgg accacggcga ggatgtccgg atcatcgtcc
     1381 gcgatgagga ctctttcgct catttgcctt ctattgccct tctcagttcg gcagcagccg
     1441 tcggcggatc atccgacccg aaaatggtcg tcccggccac gagcacgttt gctcccgccg
     1501 cgacagcctg actggcggtt tcgaggttga ttccaccatc gatctgaata tcggtcctca
     1561 atccatggtc ctcaacccat tctctggcct cctccacctt ggtgaggact tcgggcatga
     1621 agccctggcc gccgaatccg ggctcgaccg acatgatcaa gatcagatcg ctgagctcca
     1681 tgaaaggctg gaccgcggcg aacggggtgg ccgggttgca gacgagaccg aagcccagtc
     1741 cgatttcacg ggcctgcttc gccgcgtcgg tcgggtcggg cattgcctcg atgtgcatcg
     1801 tcaccgagtc ggctcccgcc tcggccagat cggtcaggta ggcgtcgggg ttggtggtca
     1861 tcagatggca gtcgaagcgg agcgtcgtca tcggcctgat gcctgcgatg accgggatgc
     1921 caaaggagat attgggcacg aaatgcccgt ccatcacgtc gatgtgcagc cagtccgcct
     1981 ccttctccac cgccaggatc tggttctcca gacaggtgaa gtcggcggcg agcagcgatg
     2041 gtgcgatctt cagcgcggcc atgggggcga gtctatttcc tgtccggggc ccgctcaggc
     2101 gatggtcccg ctgctgcgac ggagcccgcg aagccacgat gcgcccgaca tcgctcgctt
     2161 gccggccggc aagagtgtca ccagctccat tcctccgtca cccaacccgg cgacgggcac
     2221 tccttcgacc tccatccagc gacctgtctc gggaccggtc tcccgggctc gagcctgcac
     2281 cacttggtgt cgctcaccgt ctatgacgag cgtggcccca ggttcgggcg agagcgccct
     2341 cacccggttc acctcctcgc ttcgggtgcc gccgatcctg atcacgcgct cctgcggctc
     2401 gatcttgggc gcataggtca gcccctcgtc actttgtggg acgggttctc gggttccgtc
     2461 gaggtacgag ggca
//
LOCUS       DASTXO010000398         2059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1423918, whole genome shotgun sequence.
ACCESSION   DASTXO010000398 DASTXO010000000
VERSION     DASTXO010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423918"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VFU96_08385"
     CDS             <1..802
                     /locus_tag="VFU96_08385"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5113325.1"
                     /translation="GVIITTSHQSHRAMIEQAAAAGKHVFVDKPFTNTVEDGLAAIAA
                     ARDAGVLLQVGHQRRRTPAKRRIKAMLDAGELGEVETVVANQSIPNGFKMPETAWRWD
                     PEQSPLGSMTSLGVHKIDTINYLAGPVRSVFAFTRPGRIKPIDEATVLAMELDSGALA
                     TLTTSFFTPVVNDTTVFGTDAAAWSTKGGAKLRIQGRDDPDPVEVQLEPIDELADQMA
                     AFASAIHGQIPVEVDGETGLAVIAVMEAAVESATSGRAVEVASVMAGA"
     gene            814..1680
                     /locus_tag="VFU96_08390"
     CDS             814..1680
                     /locus_tag="VFU96_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU5113326.1"
                     /translation="MEYDEILYEKRGDAAWIGINRPEVRNAFREQTLDELAHALESTR
                     EDPTIVAAVVYGVGGHFSAGGDFHAMMKLNKANSYMWNDRMLKVAMTCRNLPIPVIAM
                     VEGACVGGGHELMLWCDLVITADDATFGQTGARVGACPTVGATQYIAKLIGERRAKEM
                     IFLCKRYTGAEAAEIGLANEALPAAAVRGRVEEIVQQMKGYSSQTIRATKVSLNFDSD
                     ALYPSWQHGMELLANIWGTEESLEGMNAFLEKRPADFHQFRVRNKDALDTYLDDFDEG
                     RNQSEASQAART"
     gene            1677..>2059
                     /locus_tag="VFU96_08395"
     CDS             1677..>2059
                     /locus_tag="VFU96_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006401989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU5113327.1"
                     /translation="MTPEGALTDLRVIDLSRVLAGPLCTQTLGDLGAEVIKVERPGEG
                     DDTRNWGPPYAGPLSAYFLGINRNKKSLTLDLSRPEGRTVLDRLIRSGDVVVDNFKQG
                     TLARWGFDDAWFEENAPAVIRCSITG"
BASE COUNT          394 a          649 c          692 g          324 t
ORIGIN      
        1 cggggtgatc atcaccacct cccatcagag ccaccgggcg atgatcgagc aggcggcagc
       61 agccggaaag cacgtgttcg tcgacaagcc gttcaccaac accgtcgagg acggtttggc
      121 cgcgatcgcc gcggccaggg acgccggtgt gctgctccag gtgggacatc agcgccggcg
      181 aactcccgcc aaacgccgga tcaaagccat gctcgacgcc ggagaactgg gggaggtgga
      241 aacggttgtc gccaaccagt cgatccccaa cgggttcaag atgccggaga ctgcctggcg
      301 ttgggacccg gagcagagcc cgctgggctc gatgacctcg ctcggggtgc acaagatcga
      361 caccatcaac tatctcgccg gcccggtacg gagtgtgttc gcgttcaccc gtcccggccg
      421 gatcaagccg atcgacgaag ccacggtcct ggccatggaa ctggacagcg gggccctggc
      481 caccttgacc acgtcgttct tcacgccggt ggtgaacgac accacggtgt tcggcaccga
      541 tgccgccgcc tggagcacca aaggcggggc gaagctgagg atccagggca gggacgatcc
      601 ggacccggtc gaagtccagc tggagcccat cgacgagctc gccgatcaga tggccgcatt
      661 cgcctccgcg attcatggcc agatccctgt agaggtggac ggcgagaccg ggctcgccgt
      721 cattgccgtg atggaagccg ccgtggagtc ggcgacgtcg ggtcgagccg tcgaagtggc
      781 gtcggtcatg gccggggcct gaagggagaa gccatggagt acgacgagat cctctacgag
      841 aagcggggcg atgccgcctg gatcgggatc aacagacccg aagtgcgcaa cgccttccgc
      901 gagcagacac tcgatgagct ggcccatgcc ctcgaatcga cccgggaaga ccccacgatc
      961 gtcgccgcgg tggtctatgg ggtcggaggc cacttctccg ccgggggcga ctttcacgcc
     1021 atgatgaagc tgaacaaggc caactcctac atgtggaacg atcgcatgct caaggtggct
     1081 atgacctgtc gcaatctccc gatcccggtg atcgccatgg tggaaggcgc ctgtgtcggc
     1141 ggcggtcacg agttgatgct gtggtgtgac ctggtgatca cagccgacga cgcaacgttt
     1201 ggccagaccg gtgcccgggt cggcgcttgc ccgactgtcg gggcgactca gtacatcgcc
     1261 aagttgatcg gcgagcgccg ggccaaggag atgatcttcc tctgcaagcg gtacaccgga
     1321 gcggaggcgg cggagatcgg gctggccaac gaggcgttgc ccgccgccgc ggtgcggggg
     1381 cgggtcgagg agatcgtcca gcagatgaag ggctactcgt cacagacgat cagggccacc
     1441 aaggtctcgc tgaacttcga ctccgatgcc ctatacccct cctggcaaca cggcatggaa
     1501 ttgctcgcca acatctgggg cacggaggag agcctcgagg gcatgaacgc cttcctcgag
     1561 aaacgccctg ccgacttcca tcagtttcgg gtccgaaaca aggacgccct cgacacatat
     1621 ctcgacgact tcgacgaggg tcggaaccag agcgaggctt cgcaggccgc tcgaacatga
     1681 cacccgaggg ggccctgacc gatctcaggg tgatcgatct ctcccgggtg ctggccggtc
     1741 ccctctgcac tcagaccctg ggtgacctgg gcgcggaggt catcaaggtg gagcggcccg
     1801 gggagggcga tgacacccgg aattgggggc cgccctatgc cgggccgctc tccgcctact
     1861 tcctcgggat caaccgcaac aagaagagcc tcacccttga tctgtcccgg cccgaagggc
     1921 gcacggtgct cgatcgcctg atccggagcg gggacgtggt ggtcgacaat ttcaagcaag
     1981 gaacgctggc ccgttggggc ttcgacgacg cgtggttcga ggagaacgct ccagcggtga
     2041 tccggtgctc gatcaccgg
//
LOCUS       DASTXO010000399         3132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1386855, whole genome shotgun sequence.
ACCESSION   DASTXO010000399 DASTXO010000000
VERSION     DASTXO010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386855"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="VFU96_08400"
     CDS             <1..368
                     /locus_tag="VFU96_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HEU5113328.1"
                     /translation="YTDEEWKLVNESRKILDIPGLHVEPTCVRVPVLVGHGVVASLRF
                     ERPIEPDEATEILEAAPGVRVWADMVPTPLDSAGIDDVLVGRIRPTVGHPGGINLWAV
                     GDNLRKGAALNAVQIAELL"
     gene            complement(365..688)
                     /locus_tag="VFU96_08405"
     CDS             complement(365..688)
                     /locus_tag="VFU96_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113329.1"
                     /translation="MTAYDARLRLDGTDEAPISVLIDLSDDRLTMMAGEIEVGDWARD
                     EIRISALPDGFHVRAEGEEIILDVTDDARFALDLGLRNAPPLLRRRMSALLRNDGPTE
                     GSPPV"
     gene            813..886
                     /locus_tag="VFU96_08410"
     tRNA            813..886
                     /locus_tag="VFU96_08410"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:847..849,aa:Pro,seq:cgg)
     gene            1053..1823
                     /locus_tag="VFU96_08415"
     CDS             1053..1823
                     /locus_tag="VFU96_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113330.1"
                     /translation="MRGARQALTETFRDVSVGGIASLVSGIVVLGLGGRLVMFLSRVL
                     HPEAIGRLTENGNEIGVFTVDGTLGLLIFGGITGGLAAAPVWVVMKPWIPKRWAVVGI
                     GAVSIGGFQLVASDNPDFDILQGPAVDLVLLLTLVFVFGVVVYLLDRFLDDRLPRGDR
                     AGSMVFYALVTVLGLLFAPLGLVLQFTSGFTDRVYAPIWTAVFVCATALVTLWWWVDR
                     ARGTSEPTPVQRRLGSGFAAGAALVGLLHLTFEIVRIL"
     gene            complement(1848..2453)
                     /locus_tag="VFU96_08420"
     CDS             complement(1848..2453)
                     /locus_tag="VFU96_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOPRIM nucleotidyl transferase/hydrolase
                     domain-containing protein"
                     /protein_id="HEU5113331.1"
                     /translation="MDSPELLVVKRALADGIEPSTVVLVEGASDKAAVETLARRRGRN
                     LGDERVVIVPMGGATTIGHFLGLLGPQGHDHRLAGLCDAGEEGDFRRALEQAGMGAHL
                     TRADMEANGFFVCVSDLEEELIRALGPDTVIAIIEAQGELGSLRTLQRQPYHRDRSVE
                     QVLWRFMGTRSGRKTIYARLLVEALDLDRVPRPLDGVLDYV"
     gene            2519..2962
                     /locus_tag="VFU96_08425"
     CDS             2519..2962
                     /locus_tag="VFU96_08425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5113332.1"
                     /translation="MIRYLHPDDVDDLIRVWLASTTPGQSFLPEDHWREMEPTVRHEL
                     LPAADTWVMEKDGALVAFMSVIDDLIGGLFTHPDHQGRGHGRALVEHARRFHDPVFVE
                     VFEANEKAIGFYRSCGFVDHDRRIEEGSGLPLLILRLGARGWTTS"
BASE COUNT          503 a          996 c         1017 g          616 t
ORIGIN      
        1 ggtacaccga cgaggagtgg aagctggtca atgagtcgag gaagatcctc gacatccccg
       61 gcctgcacgt ggagccgacc tgtgtccggg tgccggtgtt ggttggacat ggagtagttg
      121 cgtcacttcg cttcgagcgc ccgatcgagc cggacgaggc gaccgagatc ctcgaggccg
      181 cgccaggagt ccgagtttgg gccgacatgg tgcccacccc tctggactcg gccgggatag
      241 atgacgtgct cgtggggcgg attcgcccaa cagtgggtca tccggggggc atcaacctct
      301 gggccgtagg cgacaatctg cgaaagggcg ccgccctgaa cgccgttcag atagccgagc
      361 tgctctagac cggaggtgat ccttcggtcg gcccatcgtt ccggagcagg gcagacatgc
      421 ggcgtcgcag cagtggtggg gcgttgcgta accccaggtc gagagcgaat cgggcatcgt
      481 cggtcacgtc gaggatgatc tcctcccctt cggcacggac gtggaaccca tcgggcaggg
      541 cagagatacg tatctcgtcg cgcgcccaat cgccgacctc gatctcccct gccatcatcg
      601 tcaggcgatc gtcgctgagg tcgatcagaa cactgatcgg tgcttcgtcg gttccgtcca
      661 atcggagccg ggcgtcgtag gcggtcatca tcctcccctg agctgtctgc gccagctaga
      721 taagagtatc ccgggtttcc ctggaggaaa gaggttcttt cggcaaccga atgggcatgg
      781 tcccatgcct cgcctaccat tctgagctca atcgggacgt ggcgcagctt ggtagcgcgc
      841 agcgttcggg acgctgaggt cgtgggttcg aatcccgccg tcccgactca gaaaccccag
      901 gtcagggcca tctcaacggg tggccctgat tcgcgccggg acctgtttca taggaagacc
      961 gtcgaccgaa tagcttgacg gacgtgtcgc ggagacaggc gtagcggatg gcgagcggtc
     1021 gacctgcaga tgcccgtgcg cccacccctc cggtgagggg tgcccgtcag gcactgacag
     1081 agacctttcg tgacgtctcc gtcgggggta tcgccagcct cgtctccggg atcgtggtcc
     1141 tgggccttgg tgggcgtctg gtcatgttcc tcagccgggt cctgcacccg gaggcgattg
     1201 ggcggctgac cgaaaatggc aatgagatcg gggtgttcac agtcgacggc acattgggac
     1261 tcctgatatt cggtgggatc accggcgggc tggccgcggc tccggtgtgg gtggtgatga
     1321 aaccgtggat accaaagcgg tgggcggttg tcggcatcgg ggcggtatcc atcggcgggt
     1381 tccaactggt cgcttcggac aaccctgact tcgacatcct ccaggggccg gcggtcgatc
     1441 tcgtcctgct cctgactctg gtgttcgtgt ttggggtcgt tgtctatctg ctggatcggt
     1501 ttctcgatga ccgactgcca cggggagacc gggcggggtc gatggtcttc tatgccctgg
     1561 tgaccgtcct cggactcctg ttcgcacccc tcggtctggt gcttcaattc acgtcgggat
     1621 tcaccgatcg cgtatacgcc ccgatctgga ccgccgtctt cgtgtgtgcc actgccctgg
     1681 tcaccctctg gtggtgggtc gatcgggcca gaggcacgtc agagccgacg ccggtccagc
     1741 gtcgactcgg ctcgggcttc gctgccggcg cggcactggt tggccttctt catctcacct
     1801 tcgagatcgt gcgaatcctc tgagcaaccg gccctgggcc gacgtggtca aacgtaatcc
     1861 aggacaccgt ccaatggtcg cgggacccga tccaggtcga gagcctcgac cagcaatcgg
     1921 gcgtaaatcg tcttccgccc tgatcgcgtc cccatgaatc tccagagaac ctgttcgacg
     1981 cttcggtcgc ggtgataggg ctgtcgctgc agggtgcgta gggacccgag ctctccctgc
     2041 gcctcgatga tcgcaatgac ggtgtccggg ccaagggcac gaatcaactc ttcctccagg
     2101 tcggagacgc agacgaagaa tccattcgcc tccatgtcgg cgcgggtcag gtgggcaccc
     2161 attccggcct gctccaacgc gcgccggaaa tccccctcct ccccggcgtc gcagagaccg
     2221 gcgagtcggt ggtcgtgtcc ctggggaccg agcaggccca ggaagtgacc gatggtcgtc
     2281 gctccgccca tggggacgat gacaactcgc tcgtcgccta ggttccgacc gcggcgtctc
     2341 gccagggttt ccacggccgc cttgtcgctg gccccttcca cgagcacgac tgtgctcggc
     2401 tcgatgccgt cggccagagc ccgtttcacc acgagcagtt ccggtgaatc catgcccatc
     2461 aggttggcac tccgttgtga ttcggccttc cgattgtgga tgcccttagc gttccccggt
     2521 gatccgctat ctccatcccg atgatgtcga cgacctcatc agggtatggc tcgcctccac
     2581 gacccccggt cagtccttcc tccccgagga tcattggcgg gagatggagc ccactgtccg
     2641 gcacgagctg ctccctgccg ccgacacctg ggtgatggag aaagacggcg cattggtggc
     2701 gttcatgtcg gtgatcgacg atctcatcgg cgggctcttc acccaccccg accatcaggg
     2761 gaggggccat ggccgcgctc tcgtcgagca cgctcggcgg ttccacgacc cggtcttcgt
     2821 cgaggtgttc gaggccaatg agaaggctat cggcttctat cggtcctgcg gattcgtcga
     2881 ccacgatcgc cgcatcgagg agggatccgg gctccccttg ctcattctcc ggcttggcgc
     2941 ccgcggctgg actacgtcat gacctttcac ggcgacatgg agagaccctc cgcgatcaag
     3001 cggagggtca gacgggcgag gtggcgccga gggccacccg cagctgatgg gacgagaagg
     3061 gcttgaccac ccatgcgtcg gccccggctc gtttggccag gaacccgtcg acctcccgat
     3121 cgaggagaat ca
//
LOCUS       DASTXO010000400         5496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1386925, whole genome shotgun sequence.
ACCESSION   DASTXO010000400 DASTXO010000000
VERSION     DASTXO010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386925"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VFU96_08430"
     CDS             <1..765
                     /locus_tag="VFU96_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU5113333.1"
                     /translation="KVIELDRARNNVVLSRRAYLEEAQAEERNAFLDGLGEGEVRDGV
                     VSSVVNFGAFVDLGGMDGLVHVSELSWQHVNHPGELVKVGDKVQVKVLEVDRDRERIS
                     LSIRQTSDDPWDVFAREHEVGDVIDGVVTKTVPFGAFVSVADGVEGLVHVSEIASHRV
                     ESPELELSIGQQVKVKITEKEDERRRISLSIKQASPDWKEREAQMEEQKKGRRRPQKE
                     FEREFGPSEEERPPMPVDASLEAILQELKERGIGRK"
     gene            993..1919
                     /gene="cpaB"
                     /locus_tag="VFU96_08435"
     CDS             993..1919
                     /gene="cpaB"
                     /locus_tag="VFU96_08435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEU5113334.1"
                     /translation="MGRRAIVLLVALILAGLAAWAVWNYLQNVENEIVEGQEIVEVYR
                     AGQPIAEGTDGAILLSDFNSGGELITLSEDQAEDVPVGAITTPEQLNQVLTDRVAAGP
                     IAAGSILNESQWASITVNVVPLAEEIPSGKQAMTISTDNIRGINGFVEPGDRVNMIIT
                     IDIPEDLLPDELAGIAEEPVAPEEGAPAEEATASVTYTRFVLQGLPVMATGRDIRPEE
                     DAPETVEVDPETTGDTTPDGQPVPEEEELGNDTTFTLEVTPEQAERIAYATENGSVWL
                     TLVPGDFVEVETTGVTTLNLFGGNLLDDIFGN"
     gene            1939..3114
                     /locus_tag="VFU96_08440"
     CDS             1939..3114
                     /locus_tag="VFU96_08440"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5113335.1"
                     /translation="MAEHNLLVVDKDDDFVSSAKELFEGRLPVARSIEEASHTAESGE
                     LRMLLLGPSFSNDESLEQIRSLHNQDPALVLMMVAETVTADLLRGAMRAGVSDVIEAP
                     LDEAKIEAAIEQFGHDVLKRRSAAKPTTEPVRSEKGSIVTVTSAKGGSGKTVTATNVA
                     LLLNRLPDKKVVLVDADLQFGDVCLLLQLEPRFTMVNAAHELHHLDVEMLDSLLTEHP
                     SGLKVLAAPLEPAFADDITTAGLMQMLDLLQETYDYVVVDTASMLDELILSLIEKADH
                     VLMVVDMDLPSVKNAKLALETLRLLKFSTQNVQLVMNRNNSKAKLDNKEIESALKMEI
                     SASVPSDAAVPNSVNEGRPIVETDPKGKVAKGFESVAELIAGKIPESTSKGGLFGRK"
     gene            3131..4489
                     /locus_tag="VFU96_08445"
     CDS             3131..4489
                     /locus_tag="VFU96_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEU5113336.1"
                     /translation="MPSLSERVAAARQTETQEVSSNRSDALGELRGRLHFKVVEELGP
                     TLYDRQMSDAELRLRVLEMLEWAVDQEQGLPLTSADRRALLNEIASDVLGYGPIDPLL
                     NDPDITEVMANGPYDIYFEKRGKIILSDVKFVDEVHLRRIIDKIVGQIGRRVDEATPM
                     VDARLPDGSRVNAVIHPLAIGGPFLTIRKFAVDPYQEADLINFGTITQRVADFARACV
                     RGRLNVLISGGTGSGKTTTLNVMSSYIPGDERIVTVEDAAELQLHQEHVLTLESRPAN
                     IEGKGAIAIRDLVRNTLRMRPDRIVVGEVRGAEALDMLQAMNTGHDGSLTTVHSNSPR
                     DTLSRIETMVLMAGFDLPVRAIREQIAAAVDLIIHVSRLKDGTRRVTHVTEVEGMEGE
                     IITLQDIFLFDYGMGVDEDGVYKGRLKATGIRPTFSQDLENQGIRLPADLFDPEPFAK
                     GR"
     gene            4486..>5496
                     /locus_tag="VFU96_08450"
     CDS             4486..>5496
                     /locus_tag="VFU96_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEU5113337.1"
                     /translation="MSLRRLSALVAVLVLGLSTAAVAQTPAPEVEIVDVNGSRYAEGG
                     QTQMVVEFRNLPQAPDPAQLSITVDGQPVSDLEVAPLGESSVPVGVVLVIDTSGSMLG
                     DPMDAAKSAANSFIDQKRPEDAIAIVSFADTAVVQTGFTTNAEVLHDRVNALEADGET
                     AFNDGVILGMSQFEGVPENQLNNMIVLSDGEDTASVATPEEAIAAVEAGDARVFGVAL
                     ESPDFNPDAVAAVAEAGGGLFLSTPDPAQLSGLYSQISREISNTLVARFVSPVAAPGE
                     AEFAVSYPGGLTSTVVVPVSGYATTTTIAPTTTTVPVPITGSVVESNSPLSITNLALI
                     AAA"
BASE COUNT         1083 a         1762 c         1693 g          958 t
ORIGIN      
        1 aaggtgatcg agctcgaccg tgccaggaac aatgtcgtcc tctcccgtcg ggcctatctg
       61 gaagaggccc aggccgaaga acgcaacgcg ttcctcgacg gcctcggcga gggtgaggta
      121 agggacggcg tggtctcatc agtcgtaaat ttcggggcgt tcgtggacct cggggggatg
      181 gacggcctcg tccacgtgtc cgagctctcg tggcaacacg tcaatcaccc cggcgagctc
      241 gtcaaggtcg gagacaaggt gcaggtcaag gttctggaag tcgatcgcga tcgggagcgg
      301 atctcgctgt cgatccgaca gaccagcgac gatccatggg acgtgttcgc ccgtgaacac
      361 gaagtaggcg acgtgatcga cggggtcgtg accaaaacgg tgccgttcgg cgctttcgtc
      421 tccgttgccg acggagtcga agggctggtg cacgtatccg agatcgcgag tcatcgggtc
      481 gaatccccgg agctcgagct ctccatcggc cagcaggtca aggtgaagat caccgagaag
      541 gaagacgagc ggcgccgaat ctcgctgtcc atcaagcaag cctctcccga ttggaaggag
      601 agggaggcgc agatggagga gcagaagaag ggccgccgcc ggccgcagaa ggagttcgag
      661 cgtgagttcg gcccttcgga ggaagagcgt cccccgatgc ctgtcgatgc ctccctcgag
      721 gcgatcttgc aggagttgaa ggagcgcggc atcggccgca agtgaccctg gggtcacacc
      781 accaataacc aggttcataa gtcgcgcata tggggcgacc gaactatgga acttgttggg
      841 aaaccctcaa gactcatcac atacggtccg aatcattcct gtggaatcgg acttgaagca
      901 atgggatggc gcctgcccat cggtagcatg cctcgcggca tgcatccgga ccggatcggc
      961 ggcattccca ggggacatac gaaggggact caatgggtag gagagccatc gtgttgctag
     1021 tggccttgat actggccggg ctagcagctt gggcggtttg gaactatctc cagaacgtcg
     1081 aaaatgaaat cgtggagggt caggagatcg tcgaggtcta ccgcgccggc cagccgattg
     1141 cggaaggcac ggacggagcg atcctcctct ccgacttcaa cagtggcggc gagctgatca
     1201 cactcagcga agatcaggcc gaggatgtgc ctgtcggtgc catcaccacc cccgagcagc
     1261 tgaaccaggt gctcaccgat cgtgtcgcag ccggcccgat cgctgcgggg tcgatcctca
     1321 acgaatcaca atgggcttcg atcacggtga acgtggtccc gctcgccgag gagatcccct
     1381 cgggcaagca ggccatgacc atctcgaccg acaacatcag gggtatcaac ggcttcgtcg
     1441 agcccgggga tcgggtcaac atgatcatca cgatcgacat ccccgaggac ctgctccccg
     1501 atgagttggc cggcatcgcc gaggagccgg tcgctccgga agagggtgct cccgccgagg
     1561 aggccacggc ttcggtcacc tacacccggt tcgtcctgca gggcctcccg gtcatggcca
     1621 ccggtcgtga catccggccc gaggaggatg ccccggagac ggtggaggtc gatccagaga
     1681 ccaccggaga caccactcct gatggccagc ccgtccccga ggaggaggaa ctgggcaacg
     1741 acacgacctt cacactcgaa gtcacgccgg agcaggcgga gcggatcgcc tatgccaccg
     1801 agaacggctc ggtttggctg accctggtcc ccggcgactt cgtggaagtc gaaaccaccg
     1861 gtgtgacaac tctcaacttg ttcggcggga atctgctgga cgacatcttc ggaaactgac
     1921 agaggttcga cgatgacgat ggctgagcac aacctgttgg tcgtggacaa ggacgatgac
     1981 ttcgtctcct cggccaagga gctcttcgag ggccgactcc cggttgctcg ctccatagag
     2041 gaagcgagtc acaccgcaga gtccggcgaa ttgaggatgc tcctgctcgg cccatcgttc
     2101 tcgaacgacg agtcactcga gcagatccgg agcctgcaca atcaagaccc cgccttggtc
     2161 ctgatgatgg tggcggagac ggtcacggcc gatctcctcc gcggtgcgat gcgggccggc
     2221 gtatcagatg tgatcgaggc cccgctcgac gaggccaaga tcgaggctgc gatcgagcag
     2281 ttcggccacg acgtcttgaa gcgccgcagc gctgccaagc cgaccacgga gccggttcgg
     2341 tccgagaagg gctccatcgt caccgtcacc tcagccaagg gtgggagtgg caagacggtt
     2401 accgctacca atgttgccct tctcctcaac aggctccccg acaagaaggt ggtcctggtc
     2461 gacgccgacc tccagttcgg cgatgtctgc ctgctgctgc agctcgagcc gaggttcacg
     2521 atggtcaatg ccgctcatga gctccatcat ctcgacgtcg agatgctcga ctccttgctc
     2581 accgagcatc cgagtgggct gaaggtcctc gccgctccgc tcgagcccgc attcgccgac
     2641 gacatcacca ctgccggtct gatgcagatg ctcgatctcc tccaggagac ctacgactac
     2701 gtggtcgtcg acaccgcctc gatgctcgac gagctgatcc tgtccctgat cgaaaaggcg
     2761 gaccacgtcc tgatggtggt cgacatggac ctcccctcgg tgaagaacgc caagctcgcc
     2821 ctggagacat tgcgactgct caagttttcg acccagaacg tccagctggt catgaaccgg
     2881 aacaactcga aggccaagtt ggacaacaag gaaatcgaaa gcgccctgaa gatggagatc
     2941 tcggcttccg tcccttcgga cgcggccgtc ccgaactccg tcaacgaggg gcgccccatc
     3001 gtcgagaccg atcccaaggg caaagtcgcc aagggattcg agagtgtcgc cgaattgatc
     3061 gccggaaaaa tccccgagag cacgtccaag ggcgggctct tcggcaggaa atagtggaag
     3121 gtagatagaa gtgccatcgc tttctgagag agtcgcagcc gccaggcaga cagagacaca
     3181 agaggtcagc tccaaccgct cggacgccct cggcgaactg cgcggtcgtc tccacttcaa
     3241 ggtggtggag gagctgggcc cgaccctgta tgaccggcag atgtcggacg ccgagctgcg
     3301 tcttcgcgtc ctcgagatgt tggaatgggc ggtcgaccag gagcaggggc ttccgctcac
     3361 ctccgccgat cgacgtgccc tgctcaacga gatcgcctcc gacgtcctcg gatacggtcc
     3421 catcgacccg ctgctcaacg acccggacat caccgaggtg atggccaacg gcccctatga
     3481 catctacttc gagaagcggg gcaagatcat cctgtccgat gtcaagttcg tcgacgaggt
     3541 gcacctgcgc cggatcatcg acaagatcgt cggtcagatc ggccgccgtg tcgacgaggc
     3601 aaccccgatg gtcgacgccc gcctccccga tggctcccgt gtcaacgccg tcatccatcc
     3661 gctggccatt ggtggcccgt tcctgacgat ccgaaagttc gcggtcgacc cctaccagga
     3721 agccgacctc atcaacttcg gcaccatcac ccagcgtgtg gccgatttcg cccgggcctg
     3781 tgtccgcggc cggctcaacg tcttgatctc aggtggtacc ggctccggca agacgacgac
     3841 cctcaacgtg atgtcctcgt acatcccggg cgatgagcgg atcgtcacag tcgaggacgc
     3901 cgccgagctc cagctccacc aggagcacgt gctcacactc gagtcacgcc cggccaacat
     3961 cgagggcaag ggcgccattg ccatccgcga cctggtcagg aacaccctgc gtatgcgtcc
     4021 cgaccggatc gtcgtcggtg aggtccgtgg cgcagaggcc ctggacatgc tccaggcgat
     4081 gaacacgggt catgacggct cgctgaccac ggtccactcc aacagcccta gggacacact
     4141 ctcccgtatc gagacaatgg tgctcatggc ggggttcgat ctgccggtgc gagccattcg
     4201 tgagcagatc gccgccgccg tcgacttgat catccacgtc tcccgtctca aggacggaac
     4261 ccggcgtgtc acccatgtca ccgaggtaga aggcatggag ggcgagatca tcacccttca
     4321 ggacatcttc ctcttcgact acggcatggg tgtcgacgaa gacggcgtct acaagggtcg
     4381 tctcaaggcg accgggatcc ggcccacctt ctcccaggac ctcgagaacc agggcatcag
     4441 gctccccgcc gacctgttcg atcccgagcc gttcgcgaaa ggccgatgag cttgagacgt
     4501 ctctctgccc tggtggccgt gctcgttctt gggctgagca ccgccgccgt tgcccagacc
     4561 ccggcccccg aagtcgaaat cgtcgacgtc aacggctcga ggtacgccga aggcggccag
     4621 acccagatgg tggtcgagtt ccgcaatctt ccccaggccc cggatccggc tcagctgtcg
     4681 atcaccgtcg acgggcagcc cgtgtccgac ctggaagtcg cccccctcgg tgaatccagc
     4741 gttcctgtgg gcgtcgttct cgtgatcgac acctcgggct cgatgctggg agatccgatg
     4801 gatgccgcca aatccgcggc caacagcttc atcgatcaga agcgccccga ggacgccatc
     4861 gccatcgtca gcttcgccga cactgccgtg gtccagaccg gcttcaccac gaatgcagaa
     4921 gtgctgcacg atcgcgtcaa tgcgctggag gctgacggtg agacggcttt caacgacggt
     4981 gtgatcctcg ggatgtccca gttcgagggt gtccccgaga atcagctgaa caacatgatc
     5041 gttctcagcg acggcgagga caccgcatcg gtcgccaccc cggaggaggc gatcgccgca
     5101 gtcgaggcgg gcgatgctcg tgtcttcgga gtggccctcg agtctcccga tttcaacccc
     5161 gacgcggtcg ccgctgttgc ggaggccggc gggggcctgt tcctgtccac acccgatccc
     5221 gctcagttgt cggggctcta cagccagatc agtcgtgaga tcagcaacac cttggtcgcg
     5281 cgcttcgtct caccggtcgc cgcaccgggg gaggccgagt tcgccgtaag ctatccgggc
     5341 ggattgactt cgacagtcgt ggttccggtc tccggatatg ccacgaccac gaccattgca
     5401 cctacgacga ccacggtccc cgttccgatc accggcagtg tcgtcgagag caattctccg
     5461 ctgagcatca ccaacctggc cctgattgct gcggcc
//
LOCUS       DASTXO010000401         2007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_60737, whole genome shotgun sequence.
ACCESSION   DASTXO010000401 DASTXO010000000
VERSION     DASTXO010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60737"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VFU96_08455"
     CDS             <1..972
                     /locus_tag="VFU96_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113338.1"
                     /translation="GDATVLTEVLRRLHRAFPHVPWLIVGKEISIEDVRLSLEKLADR
                     FLEHPEMVFVVTNMSYREAPHLTPLREEAEAELKWTTVPLEGTTAFDFSRQIRGLYDS
                     LVDDWAVRTSPKTGNPVYVRPSVLVLYRKDREFILGDVIPRRGTRPGLSYQLVIASQP
                     YRARTSVEQKMGTVIVPLARVLAPGGRMVVVHSHGNDPGLEIVRGVWPEEDPFQTDRH
                     VLATAAPDHFVEPGDEDLTFERATDAEAVFRYDLHAMPSELQEHIGTSLIVAAWNAAT
                     YVAQIDEERLSEAVGSGRYVPATRAVIQEYEGVWFNDEAFVISRTRG"
     gene            1031..>2007
                     /locus_tag="VFU96_08460"
     CDS             1031..>2007
                     /locus_tag="VFU96_08460"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEU5113339.1"
                     /translation="MSERPPFRADHVGSFLRPKTVVDARAQRAEGEIGPEELREVEDE
                     AIAEVVRQQEDVGLKGITDGEFRRFMFHVDFLEQLDGVTVTEGNFLARFHRLDGSEIE
                     FSPPTMGVDAPIRHARPIQGADFDYLSSQVTETPKVSIPAPSMLHFRGGRQGISEDVY
                     PDLEQFFDDLTNAYREEIADLASRGARYVQMDDTNLAYLCDPEVRERTAARGDDPDEL
                     TRLYSRLVNDAFAGRPDDMVAAIHLCRGNFQSAWVTKGGYEPVAEILFNEMDVDAFFL
                     EYDDERSGDFAPLRFVPEGKMVVLGLMSSKLPDVEDADEVKKRIDEAAE"
BASE COUNT          377 a          610 c          660 g          360 t
ORIGIN      
        1 ggggatgcca cggtgctgac cgaagtgctg cgacggcttc atcgggcttt tccacatgtc
       61 ccttggctga tagtgggcaa ggagatcagc atcgaggacg tgcggctctc tctagagaaa
      121 ctcgccgacc gcttcctcga gcaccccgag atggtcttcg tggtcaccaa catgtcctac
      181 cgggaggccc cccatctgac cccgctgcga gaagaggcag aagccgagct caaatggacc
      241 accgtgcctc tcgagggcac gacggccttc gatttctccc gtcagatcag gggtctctac
      301 gactccctgg ttgacgactg ggcggttcgc acgagcccga agacggggaa tcccgtgtac
      361 gtccgtccat cggtgctggt cctctatcga aaggaccggg aattcatcct cggcgatgtc
      421 atcccgagaa ggggcacgcg cccggggctg agctatcagc tggtcatcgc ttctcagccc
      481 taccgggctc gcacctcagt cgagcagaag atgggcacgg tcatcgtccc cctggccagg
      541 gtcctcgctc ccggtggccg gatggtcgtg gtccactccc acggcaacga ccctggcctg
      601 gagatcgtca gaggggtctg gcccgaggag gacccgtttc agaccgaccg gcatgtcctg
      661 gcgacggcgg ctccggatca cttcgtcgag ccgggtgatg aggatctcac cttcgagcga
      721 gcgaccgacg cagaagccgt gtttcgctat gacctccacg ccatgccctc ggagttgcaa
      781 gagcacatcg gcacctcgct gatcgtggcc gcgtggaacg cggcgaccta cgtcgcccag
      841 atcgacgagg agcggctgtc cgaggcggtg ggctcgggtc gttatgtgcc ggccacccgg
      901 gcggtgatcc aagaatacga gggcgtttgg ttcaacgacg aggccttcgt catatctcgc
      961 acgcggggct gatcgtcgaa accgagagcg gtagcctcgc aatgatcgag acccgttcga
     1021 aggagatgaa atgagcgagc ggccaccctt ccgggcagat cacgtcggca gcttccttcg
     1081 cccgaagaca gtggtggacg cccgggcaca acgagccgaa ggtgagattg ggcccgagga
     1141 actacgtgag gtcgaagacg aagcaatcgc cgaggtggtg cgtcagcagg aggacgtggg
     1201 actgaagggg atcacagacg gggagttccg gcggttcatg tttcatgtcg acttcctgga
     1261 gcagctcgat ggggtgacgg tcacggaggg gaactttctc gccaggttcc atcggctgga
     1321 cgggtcggag atcgagttct cgccgccgac gatgggtgtc gacgcgccga tccgccatgc
     1381 gcggccgatc cagggggcgg atttcgacta tttgtcctca caggtgaccg agacgccgaa
     1441 ggtctcgatc ccagcgccct cgatgctcca tttccgtggt gggcgacagg ggatcagcga
     1501 ggacgtctat ccggatctcg agcaattctt cgacgacctg accaacgcct atcgggaaga
     1561 gatcgccgat ctggcgtccc ggggggcgcg gtatgtccaa atggacgaca ccaacctcgc
     1621 ctatctctgc gatcccgaag tgcgggagcg gactgccgcc cgcggcgatg acccggatga
     1681 gctgaccagg ctctacagcc gtctggtcaa cgacgccttc gccggccggc ccgacgacat
     1741 ggttgccgca atccatctct gccggggaaa tttccagagt gcttgggtga ccaagggtgg
     1801 atacgaaccg gtggccgaga tcttgttcaa cgagatggat gtcgacgcct tcttcctcga
     1861 gtacgacgac gagcgctccg gcgacttcgc cccccttcgc ttcgtccccg aggggaagat
     1921 ggtcgtgctc gggttgatga gctcgaaact gcccgacgtc gaggacgccg atgaggtgaa
     1981 gaagcgaatc gacgaggcgg ccgaata
//
LOCUS       DASTXO010000402         2451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_650514, whole genome shotgun sequence.
ACCESSION   DASTXO010000402 DASTXO010000000
VERSION     DASTXO010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650514"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="VFU96_08465"
     CDS             <1..740
                     /locus_tag="VFU96_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5113340.1"
                     /translation="GTELEAKTVVSAVDPRRTFMQLVDPRELPADLVDNIRRFKFQGT
                     SAKVNFALDANPTYPGLEGRSDQYRGFINMGPSLDYLERAFDDAKYGWYSQKPYIDGC
                     IQSNVDPDMAPPGKAVMSCFIQYAPYHLKESDWDSERDRFGDVVQKTLEGFFPGFSDL
                     VLHREVVTPLDIERVVGLTEGNIFAGEFLAPQMFFFRPAPGWNQYRTPIEGYYQCGSG
                     THPGGCVMGAPGRFASQQILGDLAKKK"
     gene            796..2166
                     /locus_tag="VFU96_08470"
     CDS             796..2166
                     /locus_tag="VFU96_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113341.1"
                     /translation="MASRRGLALIVALVLALAACTESASPPQPTAPSDAFAPTPTIGA
                     PTTTPPAPTTTLDVERVVIQRVDPVSLDPLSAFEPIPMGDWLWEPKTSPDGRFLAAAV
                     GDDAAENQLRLVDLQSWVPATTWATFPDLIVHVDDEGTVYFVTYYEAPQLHVSVKDGT
                     GSTVIADLPEGLSIWGADMIGEQVVLFGTRIQSADGSEAALVATVDVGSHEVTEIDLP
                     EVHVGPVEPVSQGPWASYLYNSPSFTIDQTGNRVLVAHANESSVTEVDLATGQAIRHD
                     LADATVTPETGTRRWGVVSPDGRFLYVASRDVELIEDDDDWSVNTIPVGVAAIDTATW
                     EVVARTDEPISDIYISPAGDRLVASGYTTEESDSVSVSDSTGLYVLDPTDLTALTHYP
                     SERPDQNWGAVTFSSAGAIAYAWTWLGAPQIRALELSSGDTLSTADGVETLAMIGPAG
                     VLASNR"
     gene            2176..>2451
                     /locus_tag="VFU96_08475"
     CDS             2176..>2451
                     /locus_tag="VFU96_08475"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEU5113342.1"
                     /translation="MLEKRPELTAQLDEDLVGWLTTVTPDGQPQSSVVWFLRDADDLL
                     VYSRPDATKLTNIATNAKVAFNLRGDDVADAVATFEATAMIVDSPTPP"
BASE COUNT          459 a          799 c          744 g          449 t
ORIGIN      
        1 acgggaccga actcgaggcg aagaccgtgg tcagcgcggt cgacccccgc cgcacgttca
       61 tgcagctggt cgatccccgc gagcttcccg ccgatctggt cgacaacatc cgccgattca
      121 agttccaggg gacctcggcc aaggtcaact tcgccctcga tgccaatccc acctacccgg
      181 gtctcgaggg ccggtcggat caataccgtg gtttcatcaa catgggcccc tcgctcgact
      241 atctggagcg tgccttcgat gacgcaaagt acggctggta ctcgcagaag ccatatatcg
      301 acggctgcat ccaatccaac gtcgaccccg acatggcgcc tccgggcaag gcggtgatgt
      361 cctgtttcat ccagtacgcg ccataccact tgaaagagag cgactgggac agcgagcgag
      421 atcggttcgg agacgtcgtg cagaaaacgc tcgaaggatt cttccctggg ttcagcgacc
      481 tggtgctgca ccgggaagtc gtcaccccgc tcgacatcga gagagtcgtc ggcctcaccg
      541 aaggcaacat cttcgccggc gagttcctcg ccccacagat gttcttcttc cgccccgccc
      601 cgggctggaa ccagtaccgc accccgatcg agggctacta ccaatgcggc tcaggcaccc
      661 accccggcgg ctgcgtgatg ggtgcgcccg gccgcttcgc ctcccaacag atcctcgggg
      721 acctggccaa gaagaagtag gcaggaaccg gtgtcgagtg ggcgccgttg atagcctcgt
      781 cccttggaga agcacatggc gtcacggcga ggactggccc ttattgtcgc cttggtcttg
      841 gccctggctg cctgcacgga atcggcctcg ccaccgcaac cgaccgcccc gtctgatgcc
      901 tttgccccaa caccaacgat tggggcgccg accacgacac ccccggcgcc cactacgacc
      961 ctcgacgtcg aaagagtcgt gatccagcgg gtcgatccgg tatcgctcga tccgctgtcc
     1021 gccttcgagc caatcccgat gggcgattgg ctctgggagc cgaaaacgtc acccgacggc
     1081 cgctttctgg ctgccgctgt cggcgacgac gctgccgaga atcaattgag gctggtcgac
     1141 ctccaatcct gggttccggc cacgacctgg gccacgtttc cggatctgat cgttcatgtc
     1201 gatgatgagg gcaccgtcta tttcgtcacc tactacgagg ccccacagct acatgtctcg
     1261 gtgaaggatg gcaccggatc gacggtgatc gccgacctgc cggagggcct gtcgatatgg
     1321 ggagctgaca tgatcggtga gcaggtcgtt ctgtttggca cccggatcca gtcggctgac
     1381 ggaagcgagg ctgccctggt agccacggtc gacgtcggca gccatgaggt gaccgagatc
     1441 gacttgcccg aagtgcatgt cggcccggtc gagccggtga gtcagggccc gtgggcgtcc
     1501 tacctctaca actccccttc gttcacgatt gaccaaacag gcaatcgggt tctcgtggcc
     1561 cacgccaatg agagctcggt cacagaggtg gatctggcga ccggccaggc aattcggcac
     1621 gatttggccg atgccacggt cacgcccgaa accgggacca ggagatgggg cgtcgtgagc
     1681 cctgacggtc ggttcctcta cgttgccagt cgagatgtcg agctgatcga agacgacgac
     1741 gactggtcgg tgaacacgat cccggtcggg gtcgcggcga tcgacacggc gacttgggag
     1801 gtcgtggcca gaaccgacga gccgatatcg gatatctaca tctccccagc aggagatcgg
     1861 ctcgtcgcct ccggctacac caccgaggaa tccgactcgg tgtctgtgtc ggattcaacc
     1921 gggctgtatg tgttggaccc gaccgaccta acggccctga cccactatcc atccgagcgt
     1981 cccgatcaga attggggggc agtcacgttc agctcggcag gggccatcgc ctacgcctgg
     2041 acatggttgg gagcgccaca gatccgcgcc ttggagctct ccagtggcga cacgttgtcg
     2101 acggcagatg gtgtggaaac cctcgcaatg attgggcctg cgggagtcct cgcctccaat
     2161 cgctagcttg cctcgatgct cgagaagcga cctgagctca ctgctcagtt ggatgaggat
     2221 ttggtcggct ggctgaccac ggtcacgcct gacggtcagc cgcagagctc ggtggtctgg
     2281 ttcctccgag atgcagatga cctgctcgta tacagcaggc ccgatgcgac gaaactgacc
     2341 aacatcgcga ccaacgccaa ggttgccttc aacctccgcg gcgacgacgt ggcagatgcg
     2401 gttgccacgt tcgaggcgac cgcgatgatc gtcgattccc ctaccccacc g
//
LOCUS       DASTXO010000403         2638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_650594, whole genome shotgun sequence.
ACCESSION   DASTXO010000403 DASTXO010000000
VERSION     DASTXO010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650594"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VFU96_08480"
     CDS             complement(<1..250)
                     /locus_tag="VFU96_08480"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU5113343.1"
                     /translation="MTDLTSLLGEPGASVAVLGATDHSGKYGGIIYRDLKRRGLRVFA
                     VNPYRESVDGDPCWKSVLDLPETPTIADFVIPARRGLDV"
     gene            316..1824
                     /gene="hutH"
                     /locus_tag="VFU96_08485"
     CDS             316..1824
                     /gene="hutH"
                     /locus_tag="VFU96_08485"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006932411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEU5113344.1"
                     /translation="MTEVVVDGGPLTPGQVVEVAYRRARAVAGPDLSDRMEPARAVVD
                     AAVESGDVVYGITTGFGALASTRIDRDEAETLQYNLIRSHASGVGPPLPDPLVRAILL
                     LRARTLAQGHSGVRPIVVERLLEMLDRDILPVIPSQGSVGASGDLAPFAHMALPLIGE
                     GRVKVDGTIVPTAGSGLASLALQAKEGLSLLNGTEGMTAMGALSLHEARQLLIASDAA
                     CAMTIEAMMGSARPFREDLHRLRPHPGQIASARRIASLIDASPIGASHEHDFDHAVQD
                     AYSLRCAPQVHGAVADTLDHMEAVLSREMESVVDNPIVFPETGEVVSGGNFHGQPLAF
                     VLDFAAIAVAELASISERRTDRLLDPARSRGLPAFLASNPGVQSGYMIGQYVQAALVA
                     ENKVLAHPASVDTIPTSGNQEDHVSMGWGAGLKLNQILSNARKVVAIEMLCAAQGIEY
                     RSPLLPAPGTAAIVGLVRAHVPPLSEDRSLTDEIEAVAGLIADGSVAAAADR"
     gene            1821..>2638
                     /gene="hutI"
                     /locus_tag="VFU96_08490"
     CDS             1821..>2638
                     /gene="hutI"
                     /locus_tag="VFU96_08490"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEU5113345.1"
                     /translation="MSDLLITGIGHLTTHQGEPLVHAVVKIEDGSVVYAGPGADAPDQ
                     GLSPRVECEGRAVLPGFVDSHTHLVFGGDRAAEFSMKMAGATYSDVAASGGGILSTVA
                     ATRNTAEAALFETAARRVKRMIAAGTTTVEIKSGYGLDLDTELVLLQVARRIGTELPV
                     SVKTTFLGAHALPKEFQNDRAGYVDLLVEEMLPAAAPLADYCDVFVEEGAFTVDEARH
                     IFAVAEKAGMGARVHADQLTHSGGAALAAEIGAVSADHLDHVTEGDAVALAASGV"
BASE COUNT          435 a          906 c          861 g          436 t
ORIGIN      
        1 cgacgtccag cccacgacgc gccgggatca cgaagtcggc gatggtgggc gtctcgggca
       61 gatcgaggac ggacttccag catgggtcac catcgacgct ctcccggtag ggattgaccg
      121 cgaacacgcg gagccctctt cgcttgaggt cccgatagat gatcccgccg tacttgccgg
      181 aatggtcggt cgcccctagc accgcaaccg aggcgcctgg ctcgcccagg agggaggtga
      241 ggtcggtcat catgtgcccg gcacggtatc cgcctcgccc ggcttgtcgg gaatcaaccc
      301 gttaccgtcc tcgtcgtgac cgaggttgtc gtcgatggcg ggccattaac tcctggccag
      361 gtggtcgaag tcgcctatcg ccgggcacgt gcggtcgccg ggccagatct ctccgaccgg
      421 atggagcccg cccgcgccgt ggtcgacgca gcggtcgaat caggcgacgt cgtctacggg
      481 atcaccaccg gattcggcgc cctcgcctcg acgcgcatcg atcgcgatga ggcggagacc
      541 ctccagtaca acctgatccg gtcccacgcg tccggcgtcg ggccgccttt gccggacccc
      601 ctggtccggg cgatcctcct gctccgggcg agaaccctcg cacagggcca ctccggggtc
      661 cggcccatcg tcgtggagcg tcttctcgag atgctcgacc gggacatcct tcccgttatc
      721 ccctcccaag ggtcggtggg agcgtcgggc gatctcgccc cttttgccca catggccctc
      781 cccctgatcg gcgaagggcg ggtcaaggtc gacggcacga tcgtccccac cgccggtagc
      841 ggcctggctt cgctggccct gcaggcgaag gaagggcttt cacttctcaa cgggaccgag
      901 ggcatgacgg cgatgggggc actgtcgctc cacgaagccc ggcagctcct gatcgcatcc
      961 gacgccgcat gcgcaatgac catcgaggcg atgatgggaa gtgctcggcc attccgggag
     1021 gacctgcatc gattgcgtcc gcatcccggc cagatcgctt cggcgcggcg catcgcctcg
     1081 ctcatcgacg ccagcccgat cggagccagc cacgagcacg atttcgacca cgccgtgcag
     1141 gatgcgtatt cactccgctg cgccccgcag gtacatgggg cagtagccga cacgctggac
     1201 cacatggagg ccgtcctctc ccgcgagatg gaatcagtcg tcgacaaccc gatcgtcttc
     1261 ccggagaccg gtgaggtcgt ctccggcggc aacttccatg gccagcccct cgccttcgtg
     1321 ctcgacttcg ctgcaatcgc cgtagccgag ctcgcctcca tctccgaacg ccgcaccgac
     1381 cgactgctcg atccggcacg atcgaggggc ctacccgcgt ttctcgccag caacccgggc
     1441 gtgcaatccg ggtacatgat cggccaatac gtgcaggcgg cattggtcgc cgagaacaag
     1501 gtgctggctc atcccgcctc cgtcgacacc atccccacct ccggcaatca ggaggatcac
     1561 gtttcgatgg ggtggggggc cggcctcaag ctgaaccaga tcctgagcaa tgcgcgaaag
     1621 gtggtggcca tcgaaatgct ctgcgctgcc cagggcatcg aataccgcag cccccttctt
     1681 ccggctcccg gtacggcggc gatcgtgggt ctggtccggg cccatgtccc gccgctgagc
     1741 gaggatcggt cgctcaccga tgagatcgag gcggttgccg gcctcatcgc cgacggatcg
     1801 gtggctgcgg cagccgatcg gtgagtgatc tcctcatcac cgggatcggg catctcacga
     1861 cccaccaagg cgaaccgctg gtgcacgccg tggtcaagat cgaagacgga tcggtcgtgt
     1921 acgccgggcc cggcgcagac gctcccgacc agggcctatc tcctcgcgtc gaatgcgagg
     1981 gacgggcggt cctgcctgga ttcgtggaca gccacactca tctcgtcttc ggaggggacc
     2041 gggcagcgga gttctcgatg aagatggcgg gcgccaccta ctccgacgtg gccgcgagcg
     2101 gaggcgggat tctctccacg gtcgcggcaa cccgcaacac ggcggaggcc gctctgttcg
     2161 agactgccgc gagaagggtc aagaggatga tcgccgccgg gacgacgacc gttgagatca
     2221 agtccggata cggcctggac ctggacacgg agctggtgtt gctgcaggtc gcgaggagaa
     2281 tcggaaccga gctgccggtg agcgtcaaga ccaccttcct cggcgcccac gccctgccga
     2341 aggagttcca aaacgaccgg gccggctatg tcgacctcct cgtcgaggag atgctgcccg
     2401 ccgccgcccc tttggcggac tattgcgacg tcttcgtcga ggagggcgcc ttcacggtcg
     2461 atgaggcgcg ccacatcttc gcggtcgccg agaaggcagg catgggggcc cgcgtccacg
     2521 ccgatcaact gactcattcc ggaggggcgg cgctagctgc cgagatcggg gcggtcagcg
     2581 ccgatcatct cgatcacgtc accgaagggg acgctgtggc cctcgcagca tccggggt
//
LOCUS       DASTXO010000404         5828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_319326, whole genome shotgun sequence.
ACCESSION   DASTXO010000404 DASTXO010000000
VERSION     DASTXO010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5828
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319326"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VFU96_08495"
     CDS             complement(<1..294)
                     /locus_tag="VFU96_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU5113346.1"
                     /translation="MPHMQDFPLNLSTLYDRAVWLYPDQEIVSVEADRSVTRTTYGET
                     DTRVRRLATAFRALGLGQGDPVGTFAWNNRRHHELYWATANTGLVCHTVNIRLF"
     gene            complement(294..902)
                     /locus_tag="VFU96_08500"
     CDS             complement(294..902)
                     /locus_tag="VFU96_08500"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEU5113347.1"
                     /translation="MAYPEKLLSPGEQIMSEFRPHWSGIVKEAILSILALVLIVLIAV
                     QGFSWGLWAIGALILIWLILVARGFIRWFTTQHVITTERVIHRLGLVSKQGKEIPLEV
                     INDVAFNQSAWERIFGTGDLLIESAGTHGQSKYTDIPNPEAIQSLIYQVREDRQRALQ
                     SRGGPAVESSASQLATLSRLHDEGKLSDVEFEAEKQKLLGGA"
     gene            complement(1457..1996)
                     /locus_tag="VFU96_08505"
     CDS             complement(1457..1996)
                     /locus_tag="VFU96_08505"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEU5113348.1"
                     /translation="MIASDQNLALAERRALALVAICQDSLYQTGQGSESTLVDVTVIV
                     EVAAALETNAEKGVSVLAGPRIGPGRFEEILCQGKVDLVGIGENGKPLDLGRRSRTVT
                     RKLRRHVLHRDGGCTVEGCSSRYRLEVHHVLPWSHGGSTDTDNLITLCWYHHHVSVHR
                     EGLHLYRTGTSRIRLKRPR"
     gene            2809..3444
                     /locus_tag="VFU96_08510"
     CDS             2809..3444
                     /locus_tag="VFU96_08510"
                     /inference="COORDINATES: protein motif:HMM:NF021576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HEU5113349.1"
                     /translation="MTTARKRVPAAWGVVFFLIAVVLVFAVIRVTIDSQNIATGVIPE
                     EGEFDRRYAQYPILAYAHILPGVVYLVLAPLQISRRFRNRHLDLHRKMGRILVPIGIL
                     SGVLGVIFGFFFSFGGFAEASAAVVFGTWFVIALALAYSYIRAGDTVRHRRWMIRAFA
                     IGLGVGTIRIWVGLFEATALLEFGDAFGAAFWLSWTLHVIAGEIWLWRRPT"
     gene            3490..4281
                     /locus_tag="VFU96_08515"
     CDS             3490..4281
                     /locus_tag="VFU96_08515"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEU5113350.1"
                     /translation="MTVELELAPLETLSPSRAGDFKACPQLFKFRAIDRLSEPASVYQ
                     ARGTTAHLALQRLFDHPSTQRSPQLLYDLFRDAWTELRGSEEFEDLFGDTDEERQWGV
                     ESLALLTNYFTIEDPTGFDPVDRELDMLEDLDGITIRGILDRIDRDDDGRLVITDYKT
                     GKAPPERYALPAFFALKIYALLIRRRTGETPKELRLLYLNGPTLYKLEIDDRQLDAMD
                     TQLRALWNAIDRALATRNFPTRPGTLCNWCSFKEICPAFAGTIAS"
     gene            complement(4278..5549)
                     /locus_tag="VFU96_08520"
     CDS             complement(4278..5549)
                     /locus_tag="VFU96_08520"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEU5113351.1"
                     /translation="MTAAVGNEFDSPMYKEAAGQFDRVARLMGLDDNIADRLRVPQKS
                     TMVSFPFRRDTYSEVETLFGYRIQHLTTMGPTKGGIRFAPDVDLGEVAALAMLMTWKC
                     SIVGVPFGGAKGGVRVDPMGLSRAELQRVTRRFTMEIINVIGPETDIPAPDLGTNEQV
                     MAWLMDTYSQHVGHSVPAVVTGKPPALGGSVARREATGRGLMFLLPQAAGVREIDPNN
                     LSVAIHGFGNVGRYAAIAGHQMGVKVAAVSDISGALYDPDGLDVDTVAKWVDENRFLE
                     GYPDADFIDGDKIFSLPVDVLVPAAIQNVIRGDNAEEIQAKIIIEGANGPTTIEADDI
                     LREKGVLIIPDILANAGGVTVSYFEWVQDVQKYFWTENEVVARLREIMTRAFSDVHNI
                     AINEDVDLRTAALIKGIRKVADAKLVRGIFP"
     gene            5642..5779
                     /locus_tag="VFU96_08525"
     CDS             5642..5779
                     /locus_tag="VFU96_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113352.1"
                     /translation="MSYFETFETVTEPNADPFADPVRYLAAYGIEAELVAEVARLPEA
                     A"
BASE COUNT         1051 a         1936 c         1725 g         1116 t
ORIGIN      
        1 gaagagccgg atgttgacgg tgtgacagac caaccctgtg ttggcggtag cccagtagag
       61 ctcgtgatgc cgacggttgt tccaggcgaa ggtgccgacc gggtctccct gccccaggcc
      121 tagtgcgcgg aaggcggtgg cgaggcgccg caccctggtg tcggtctcgc cgtaggtggt
      181 gcgagtcacc gacctgtctg cctcgacgga gacgatctcc tgatccggat agagccagac
      241 cgcacggtca tacagcgtgg agagattgag cgggaagtcc tgcatgtgtg gcatcacgcg
      301 cctccgagca gcttttgttt ctctgcctcg aactcgacgt cgctcagctt gccctcgtcg
      361 tggagccggg acagcgtggc gagttgcgac gcggagctct ccacggccgg cccccctctt
      421 gattggaggg ctcgctggcg gtcctctctg acctggtaga tcaacgactg gatcgcctcc
      481 gggttgggaa tgtcggtgta tttgctctgg ccatgggtgc cggccgactc gatcagcagg
      541 tcaccggtgc cgaagatccg ctcccaggcg gattgattga acgccacgtc gttgatgacc
      601 tcgagtggga tctccttgcc ctgtttcgag accagaccaa gtcggtgaat gacgcgctct
      661 gtcgtgatca cgtgctgggt cgtgaaccaa cggatgaagc ctctcgcgac caggatgagc
      721 cagatgagga tgagagctcc gatcgcccac agaccccaac tgaacccttg gacggcgatc
      781 aggacgatga gcaccagggc gagaatcgag aggatcgctt ccttgacgat tccggaccaa
      841 tggggccgga attcgctcat gatctgctcg ccggggctga gcagtttctc cgggtaggcc
      901 atctgcgcag gctaccgatc ggttggtccg gatgacgtct ggcgaaacga gaaagtcccg
      961 agggagcctt ggccgaagcc tcaaccctct cgggactctc gcccattcga ggtcgggccg
     1021 gggacccaac tccgaatgga gttcttgccg atggccgaag ccgtcggcag gtgtgcgatc
     1081 cgaagatcgc gacctccccg tctccgggaa gaccttgcga gtcgtgagac tcgccgttcg
     1141 gtgactgctc gacgatcccg aaggatcccc gtccagtcgc ccgcccgtcc ctggctccct
     1201 cggtttcccg gtggctccag gttctcgcac cgcggcctcg gagcggctct cacctctccg
     1261 aacctctcgg tccgagccgc cgccgggttt ccccttcggc gtgcgtggaa acgtagaccg
     1321 gccccctgac gctggcaact ccaagcagtc attgtttttc gcgaatgatc gaaagtttcc
     1381 gcgacgtggc gcttggagtg tcgtaatcgg gtttggggca cgacgggggt tctatcgggg
     1441 cgggggaagg gcgcccctat cggggtcgtt tcagtcggat ccggctggtc cccgtccggt
     1501 agaggtggag tccttcccgg tgcactgaca cgtgatggtg ataccaacag agtgtgatca
     1561 gattgtcggt atcggtgctt cctccgtggg accaggggag gacatgatgg acttcgagcc
     1621 ggtatcggct ggaacagcct tccacggtgc agcctccatc ccgatgcaga acatgacgtc
     1681 gcagtttccg agtcacggtc ctcgatctgc gacccaaatc gagcggtttg ccgttctcgc
     1741 cgatcccgac caagtcgacc ttcccctgac agaggatctc ttcgaacctt cccggtccaa
     1801 tccggggtcc ggccaggaca ctgaccccct tctccgcatt ggtttcaagt gctgcggcga
     1861 cctcgacgat caccgtcaca tcgaccaacg tcgactctga accctgaccg gtctgataga
     1921 gcgagtcctg acagatggcg accagagcca acgctcgacg ttccgctaaa gccagattct
     1981 gatcagaagc gatgatctca tcggctttct gatctaccgc ctttttccat catccgagca
     2041 tccaaaccag gaagcccacc ccaaaacgaa gtggccaact cgtcgcaaaa tccgaaatgg
     2101 agataaccgt taccggtctt ccgttcccgt tttcgggtca accgtctgtg atcagccacc
     2161 agccgacgca gttgggcgat gtcatacccc tgatgacccg accgatgcga ttccggtaac
     2221 cgggccaaca agccagcccg atcaaaactg atctcaccag ccgccagttg ctcggccacc
     2281 tcaggagcgt cttcgagaca gctcgccgtc caacagatcg accgggccgt ctcatgcgag
     2341 acatccgccc gagccgcggt ccaatccacc aacgaccgat acccatccaa caaatgcgac
     2401 ctgcggcgac gcttctcaga gatcgcagcc aactgcaacc gccggatccg accaatctcc
     2461 aactcacccc cggcaatgat ctgatccaac gggtcctcgg agcaactccc caactccata
     2521 cccaacaacc tacgacccgg gtgtgacaga tttgccaccc ggaacgcgag aaaccccgag
     2581 aaagctttgg ccgaagccga agccctctcg gggctctcag tgccactcga ccgggaaagc
     2641 gggcccccag tcaggtggcg atccggaggg gccgaagcct ctccttcccc gacccgggcg
     2701 aacccgtgtc ggtagacggc aaagtacgaa aggcgttctc acccgtcaag ccataccggc
     2761 gccttgtttt cgcggcctag ccgatctttt cgcgtttagc cttccacgat gacaacggcc
     2821 cggaaaaggg tgccggcggc atggggcgtg gtcttcttcc tgatcgcagt tgttctcgtc
     2881 ttcgccgtga tccgggtgac catcgacagc cagaacatcg ccaccggggt catccccgag
     2941 gaaggcgagt tcgaccgtcg ctatgcccag tacccgatcc tggcctacgc ccacatcctc
     3001 cccggtgtcg tctatctggt cctagccccg ctccagatct cccgccggtt ccgcaaccgt
     3061 catctcgacc tgcatcgaaa gatggggcgg atcctggtgc cgatcgggat cctttccggt
     3121 gtcctcggcg tcatcttcgg gttcttcttc tccttcggag gcttcgccga agccagcgcc
     3181 gctgtcgtgt tcgggacctg gttcgtcatc gccctggccc tggcctacag ctacatccga
     3241 gccggagaca ccgtccgaca tcgtcgatgg atgatccggg ccttcgccat cggactggga
     3301 gtggggacca tcaggatctg ggttggactg ttcgaggcca ccgccctgct cgaattcggc
     3361 gacgccttcg gcgccgcctt ctggctctcg tggacattgc acgtcatcgc gggcgagatc
     3421 tggctgtggc gacggcccac ctgatcgatc tacccattcc tgtcacagcc cggtcttaca
     3481 ctcggcatcg tgaccgtaga actggaattg gccccactgg agaccctctc cccgagccgt
     3541 gccggggact tcaaggcgtg cccccagctc ttcaagttcc gggccatcga ccgtctcagt
     3601 gaacctgctt cggtgtacca ggctcgaggc accacggccc atctcgccct gcaacggctc
     3661 ttcgaccatc cgtccaccca gcgttcaccc cagttgctct atgacctgtt ccgagacgcc
     3721 tggaccgagc tccggggaag cgaggagttc gaagacctct tcggcgacac cgacgaggag
     3781 cgccaatggg gggtggagtc cctcgccctc ctgaccaact acttcaccat cgaggacccg
     3841 accggcttcg accccgtgga ccgggagctg gacatgctgg aggacctcga cgggatcacc
     3901 atcaggggga tactcgaccg catcgatcgc gatgacgacg gtcgtctggt catcaccgac
     3961 tacaagaccg gcaaggcgcc acccgagcga tacgccctcc ctgccttctt cgccctcaag
     4021 atctatgccc ttctgatccg acgtcgtacc ggcgagacgc ccaaggagct acgcctcctc
     4081 tatctgaacg ggcccaccct gtacaagctc gagatcgacg atcgccagct cgatgccatg
     4141 gacacccaac tccgtgcgtt gtggaacgcg atcgaccggg ccctggccac ccgaaacttc
     4201 cccaccagac ccgggacgtt gtgcaactgg tgctcgttca aggagatctg ccccgctttc
     4261 gccgggacga tcgcctccta ggggaagatc cctcgcacca gcttcgcgtc ggccactttg
     4321 cggatcccct tgatgagggc cgcggtcctc aggtcgacgt cttcgttgat cgcgatgttg
     4381 tggacgtcgc tgaaggcgcg ggtcatgatc tcccggaggc gagcaactac ctcgttctcc
     4441 gtccagaagt acttctggac gtcctggacc cattcgaaat aggacacggt gactccaccg
     4501 gcgttggcca ggatgtcggg gatgatcagg acccccttct cgcggaggat gtcgtcggct
     4561 tcgatggtgg tgggcccgtt ggcgccctcg atgatgatct tggcctggat ctcctcggcg
     4621 ttgtccccgc ggatgacgtt ttggatcgcc gccggcacca gcacgtcgac gggcagggag
     4681 aagatcttgt caccgtctat gaaatcggca tcggggtacc cctcgaggaa gcggttttcg
     4741 tccacccact tggcgacagt gtcgacgtcg aggccgtccg ggtcgtagag cgctccagag
     4801 atgtcggaga cggccgcgac cttgaccccc atctgatgac cggcgatggc ggcgtaccta
     4861 ccaacgttgc cgaagccgtg gatcgcgacc gacagattgt tcgggtcgat ctctcgcaca
     4921 ccggccgcct gaggcaagag gaacatcagc ccgcgaccgg tcgcctcccg tcgagccacg
     4981 ctgcccccaa gggctggtgg cttccccgtg accactgccg ggaccgagtg cccgacgtgt
     5041 tgggaatagg tgtccatgag ccaggccatc acctgctcgt tggtgccgag gtctggcgct
     5101 gggatgtcgg tttccggccc gatcacgttg atgatctcca tcgtgaagcg ccgggtcaca
     5161 cgttgcagct cggcacggga cagacccatc gggtcgacac ggaccccacc ctttgcgccg
     5221 ccaaagggga caccgacgat cgagcatttc catgtcatca gcatggcgag ggccgcgacc
     5281 tcgcccaggt cgacgtcggg agcgaaccgg atcccgcctt tggtcgggcc catagtggtc
     5341 aggtgctgga tccggtagcc gaacagcgtc tccacctccg aataggtgtc acgccggaag
     5401 ggaaagctga ccatggtcga tttctggggg acacggagcc ggtcggcgat gttgtcatcg
     5461 agccccatca gccgggccac ccggtcgaac tgaccggcgg cctccttgta catcggtgaa
     5521 tcgaattcgt tgccaaccgc cgcggtcacg aggccctccg ttcattcacc cgcacgccat
     5581 ccatcctatg cgccggggac ccatggggaa tgtgtcacac ccgaccgata gcttgcccga
     5641 tatgagctac ttcgagacat tcgaaaccgt caccgagccc aacgccgacc cgttcgctga
     5701 tcccgtgcgc tatctcgccg cgtacgggat cgaagcggag ctggtggccg aggtcgcccg
     5761 cctccccgaa gcggcctgac cctacgatca gtaggtggcg ttgaccgaga tccggctcga
     5821 gccggaag
//
LOCUS       DASTXO010000405         4187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1645279, whole genome shotgun sequence.
ACCESSION   DASTXO010000405 DASTXO010000000
VERSION     DASTXO010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645279"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VFU96_08530"
     CDS             <1..370
                     /locus_tag="VFU96_08530"
                     /inference="COORDINATES: protein motif:HMM:NF025109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4162 domain-containing protein"
                     /protein_id="HEU5113353.1"
                     /translation="EEAERGKAVVFSSHQLDLVEDICEEVAIINAGQIVVEGDVRDLK
                     DAAPIRHLEVELDGEIGDLFDTLEGVRQIRSDDHIHTAVIDSSIDVRSFLARAQEMGR
                     LRHFAYTTPSLSDLFREAVR"
     gene            367..1578
                     /locus_tag="VFU96_08535"
     CDS             367..1578
                     /locus_tag="VFU96_08535"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5113354.1"
                     /translation="MSSMRTVWRIAMREIREHGRSKSFVITTGVTFILVAALVVIPGL
                     IGGGTDMFTVGSIGDGNEPIVTAAEQLGNAGDEPDAEPSVSIELEEFEDREAAEAAMD
                     EGEVDAILIDGSEVVVESVGGFGESSLLSLLQQGAASVELEAVVAEGGQAAADVIEVM
                     TSDPLETTTLTGPEAGDDTRGAVAYAGLLLLYLAVLLYGNWMLAGVTEEKSNRVVEVL
                     LSSVKPWQILAGKIIGIGSLGIAQFAGTILVAFLAVNLTGAIELPSLDVAAVANLILW
                     FILGFLLFAVMYGAAGSLVSRMEDAQNVAFPMSMIAVAGFFVSIAALGDPDGTAAIVG
                     TFIPFTAPFVVPVRAALGAIPAWQYIAAVVLTIATIVGLVLVAGRIYAGGLLRFGTRV
                     KLREAWRSADA"
     gene            1598..1927
                     /locus_tag="VFU96_08540"
     CDS             1598..1927
                     /locus_tag="VFU96_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEU5113355.1"
                     /translation="MAYDEALAHRVREKLSDNPEISERKMFGGIGFMLSGNMAVGVSK
                     DELMVRIDPDDHDEALAQPGVRISEMGGRPMKGWILVAPEVTEEDSGLRRWIQAGLDF
                     AGSLPPK"
     gene            1937..2746
                     /locus_tag="VFU96_08545"
     CDS             1937..2746
                     /locus_tag="VFU96_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEU5113356.1"
                     /translation="MGYEHIEIERDGHVATLWLNRPAKLNAMSADIWRDIPVAMAEID
                     ADDSARVVILAARGPAFTVGIDIELLASLQPGDGSRAESSMQLYRTIRDLQRTASCFS
                     ESPKPAIAAIHGYCLGGGMDLITACDIRFAARDAIFSIRETRMGLVADVGTLQRLPAI
                     VGPAHTAELAYTGKDIDAGRALAIGLINEVFPTVEATLEAARETALQIAENSPLVIRG
                     IKKALAGNERRTVDQALDIAAQWNAAFLISNDLTEAVSAYLEKRKPDFTGT"
     gene            2871..4067
                     /locus_tag="VFU96_08550"
     CDS             2871..4067
                     /locus_tag="VFU96_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019819508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5113357.1"
                     /translation="MQGHKVTMLGTGLIGDFYTMTLHGPRTRDSVGVVYSRSEERGRA
                     FSDRWDIPEHTTSLEDAINHPKTDVVVVGLPNFLHEEAIALTADAGKAVLVTKPLARD
                     AAEAKRILEKVESSGVFGGYLEDLVYTPKSMKAFDSVASGAIGDVTWVRSRETHPGPH
                     SAWFWDKEMAGGGAIVDLGCHCIEIIRCFVGKENRPVEVMAWADTLVHPIQAEDNAVV
                     MIRFESGALGQFEVSWTFRGGMDLRDEVAGTEGTIWLNHFLRTGYEMYSSGGGGGYVA
                     EKLESTEGWLFPVGDEIGELGYSAMFADMFDSLEAGTPPRETIYDGYVVNAIMDASYR
                     SASSRQWEPVEMEWRGGTTARIGKTAQTHDGHAVVKEEILPDGRRKLILSDKDTGSVF
                     DLVTSG"
BASE COUNT          768 a         1267 c         1401 g          751 t
ORIGIN      
        1 agaagaggcg gaaagaggca aagccgtggt cttctcctcg catcaactcg acttggtcga
       61 agacatctgt gaggaggtcg cgatcatcaa cgccgggcag atcgtcgtcg agggagacgt
      121 gcgcgacctg aaagatgcgg ccccgatccg tcatctcgaa gtcgaattgg acggtgagat
      181 cggggacctg ttcgacacgc tcgaaggcgt gcgtcaaatc cggtcggacg accatatcca
      241 caccgcagtc atcgactcga gcatcgacgt ccggagtttt ctggcccggg cccaggagat
      301 gggacggctg cgccatttcg cctacacgac gccatcgctc tccgacctgt tcagggaggc
      361 ggtgcgatga gctcgatgcg caccgtgtgg cgaattgcca tgcgcgagat ccgcgagcat
      421 gggagatcca agtcgttcgt gatcaccacc ggggtgacat tcatcctcgt ggcggcactg
      481 gtggtcatcc ccgggctgat cggtggaggg accgacatgt tcacggtggg cagcatcggc
      541 gacggcaacg agccgatcgt taccgccgcc gagcaactcg ggaatgccgg cgacgagcca
      601 gatgccgaac cttcggtgtc gatcgagctc gaggagttcg aggatcgtga ggcggcggaa
      661 gccgcaatgg acgaaggcga ggtcgacgcg atcctgatcg acgggtcgga ggttgttgtc
      721 gagagcgtgg gagggtttgg tgagtcgagc ctgctcagcc tcctccagca gggtgcagcc
      781 tcggtcgagc tcgaggctgt cgtggctgag ggcggccagg ccgcggccga tgtgatcgag
      841 gtcatgacat ccgatccctt ggagaccacg acgctcacag gtcccgaggc gggtgacgac
      901 acccggggag cggtggccta tgcggggctc cttctcctct atctcgccgt cttgttgtat
      961 gggaactgga tgctcgccgg cgtaaccgag gagaagtcca accgcgtggt cgaggttctg
     1021 ctctccagcg tcaagccgtg gcagatcctc gccgggaaga tcatcgggat cggctcgttg
     1081 ggcatcgccc agttcgccgg gaccattctc gtggcgtttc tcgccgtcaa tctcaccggt
     1141 gccatcgaat tgcccagtct cgacgtagca gcggtggcca acctcatcct ctggttcatt
     1201 ctcgggttcc ttctcttcgc cgtgatgtac ggggctgcgg gatccctggt gagccgaatg
     1261 gaggacgccc agaacgtggc gttcccgatg tcgatgatcg cggtcgccgg gttcttcgtc
     1321 tcgatcgctg ccctgggcga tcccgacggc acggccgcca tcgtcggcac gttcatcccc
     1381 ttcaccgctc ccttcgtggt accggtcagg gcggccctcg gcgcgatccc ggcctggcag
     1441 tacatcgcag cagtcgtgtt gaccattgcg acgatcgtgg gtctggtgct ggtggcgggc
     1501 aggatctatg ccggcgggct gctcaggttc gggacgcggg tgaagctgag ggaagcctgg
     1561 cgatcggccg acgcctgagg cctagagtcg gcaaccgatg gcctatgacg aggcactggc
     1621 ccaccgggtg cgggagaagc tgagcgacaa ccccgagatc agtgagcgga agatgttcgg
     1681 tggcattggc ttcatgctct ccggaaacat ggcggtcgga gtcagcaagg acgagctcat
     1741 ggtcaggatc gatcccgacg atcacgacga ggcattggca cagccaggcg ttcgcatctc
     1801 cgaaatgggc ggtcgaccga tgaaaggctg gatcctggtc gcccccgagg tcacggagga
     1861 ggacagcggt ctccgccggt ggatccaagc cggcctcgac ttcgccggct cgcttcctcc
     1921 caaataggct gaccgcgtgg gttacgagca catcgaaatc gagcgggatg gacatgtcgc
     1981 cacgctctgg ctgaaccggc cggcaaagct caatgcgatg tcggccgaca tttggagaga
     2041 cattccggtg gccatggccg agatagacgc agacgattcg gcgcgggtgg tcatcctcgc
     2101 ggcccgcggc ccggctttca ccgtgggtat cgacatcgag ctactcgcct cgctgcagcc
     2161 aggagatggg tctcgggcgg agtccagcat gcagctctac cgcaccatca gggatttgca
     2221 gaggactgcg agttgcttct ccgaatcacc caaaccagcc atcgccgcga tccacggtta
     2281 ctgcctgggg ggcggcatgg atctgatcac cgcctgcgac attcggttcg ccgccaggga
     2341 cgcgatcttc tcgatacggg agacccgaat gggcctggtc gccgacgtcg ggaccctgca
     2401 acggctgccg gccatcgtcg ggcctgctca cactgccgag ctcgcctaca cgggaaagga
     2461 catcgatgcc gggcgggccc ttgcaatcgg cctgatcaac gaggtgtttc cgacagtgga
     2521 ggccacgctg gaggcggccc gggaaacggc tctgcagata gccgaaaact cacccctcgt
     2581 gattcgcggc atcaagaagg ctctcgccgg caacgagcgg cggaccgtgg atcaggcctt
     2641 ggacatcgcg gcgcaatgga atgccgcctt cctcatctcc aatgacctga ccgaggcggt
     2701 ctccgcctac ctcgagaagc ggaagcccga cttcaccggc acctgaacac gatcaacggc
     2761 gagcacaggt tcgtcggttc gtgtgtgtcg acctggttcc ctgcgaccac ctctgagccc
     2821 tccgatgtcg gcgattaggt tgcggtttca gacgactgag gagggatgcg atgcaaggtc
     2881 acaaggtcac gatgctcggc accggtctga tcggagactt ctacacgatg accctccatg
     2941 ggccccgcac ccgtgattca gtaggcgtcg tgtactcccg ttccgaggag aggggccggg
     3001 ctttctccga ccgatgggac atccccgagc acaccacttc cctcgaggat gcaatcaacc
     3061 atcccaagac ggacgtggtc gtcgtcgggc tccccaattt cctccacgaa gaggcgatcg
     3121 ccctcaccgc cgatgccggc aaggcggtcc tcgtcaccaa gccactggcg agggatgccg
     3181 ccgaggcgaa acgaatcctg gagaaagtcg agtcgtccgg ggtgttcggg gggtacctcg
     3241 aagacctggt gtacaccccc aagtcgatga aggcgttcga ctcggtggcc tctggggcga
     3301 taggtgacgt gacctgggtg cgttcgcggg agacccatcc cggcccgcac agcgcctggt
     3361 tctgggacaa ggagatggcc ggcggcgggg cgatcgtcga tctcggatgc cactgcatcg
     3421 agatcattcg ctgcttcgtc ggaaaagaaa accggccggt cgaggtgatg gcctgggcgg
     3481 acaccctggt ccacccgatt caggccgagg acaacgcagt ggtcatgatc cgattcgagt
     3541 ccggcgcgct cggccagttc gaggtgagct ggaccttccg tggcggcatg gacctgcgcg
     3601 acgaggtagc cggcaccgaa ggcacgatct ggctcaatca tttcctgcgc accgggtatg
     3661 agatgtacag ctcgggtggc ggcgggggtt acgtggccga gaagctggaa tcgaccgagg
     3721 gatggctgtt cccggtaggc gatgagatcg gtgagctcgg ctacagcgcc atgttcgccg
     3781 acatgttcga ctcgctcgag gcgggaactc cgcctcggga gacgatctat gacggatacg
     3841 tcgtcaatgc gatcatggac gcgtcctacc ggtctgcctc cagccggcaa tgggagcccg
     3901 tcgagatgga gtggaggggt gggacgaccg cccgtattgg gaagacggct cagacccacg
     3961 acgggcacgc cgtggtgaag gaagagatcc ttcccgatgg tcggcgcaag ctgatcctga
     4021 gcgacaagga caccggctcg gtgttcgacc tcgtcacgtc gggttgagcc ccaacctcga
     4081 cgtcttcgcc gtcgaggaat ggcctggcat cgtggcgcaa atgctcaacc ggaggatgtc
     4141 ggagcatccc ggattgaggc tgtgcctcgc cgccggaaac acgacca
//
LOCUS       DASTXO010000406         2734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_834686, whole genome shotgun sequence.
ACCESSION   DASTXO010000406 DASTXO010000000
VERSION     DASTXO010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834686"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="VFU96_08555"
     CDS             <1..797
                     /locus_tag="VFU96_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU5113358.1"
                     /translation="LDQARDARLFILDKMAEAISEPRSEMNEFAPRIESVEIPKDKIG
                     EVIGPKGAVIRELEEVTGAAIEIEETDGKGIVRIASNDGSALAAAKERVMQIAFPPEV
                     EMGAEYDGKVVNITKFGAFVNILPGRDGLLHISRLDGSKRVERVEDYLVDGQELKVRV
                     REIDRGKVSLELVEALEGATLPEPEAPRQDGGDRRRDDGRDRGGRGGGRDRGGRDRDR
                     DRGSRDRDHSPSTADTPSQPVETEAGSERRRAAQSFDEVFEEISER"
     gene            943..1632
                     /locus_tag="VFU96_08560"
     CDS             943..1632
                     /locus_tag="VFU96_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113359.1"
                     /translation="MQMRGDSGPGVKVEVLADGSRISLLHRDELIGEWDVGAIGIQSL
                     NDGFAIRAEGEEFVLRTEDDVGLAREIGIVAASPRMARKVAASHNDDAPLRADPEPVV
                     VKSNLGAIAFALGGAMALAGGFLLRDDPTLSANATSASQGFGAEGSFWFAFTLGGLLM
                     VGVAMAIARRTKWSRLASIIVVSALVVVFGLAARSATPDADHLLAYGFIAGGVVVGVA
                     VVFAGSFVEPD"
     gene            1660..2550
                     /locus_tag="VFU96_08565"
     CDS             1660..2550
                     /locus_tag="VFU96_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011395918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEU5113360.1"
                     /translation="MPELPDIVVYLEALESRVLGEVVRGVRIASPSVLRTYDPPYDAP
                     VGLEIETLQRVGKRIVFGMEDDLYLVIHLMVAGRLRWETPKKPIPKKVGLAAIDFDPG
                     TLILTEQGTHKRAGIWLSRGFEGVSELDRGGVEPLEADAAEFGSALKRENRTLKRALT
                     DPRIFSGIGNSYSDEILWQARMSPVKRTSQMTDEEVEQLRQAVVQALTEWTNRLRDEV
                     GENWPAKVTAFRDDMAVHGKYGQPCPRCGSKVQRIVHADNETNYCATCQTGGKLLADR
                     SLSRLLKDDWPKTIEELEEG"
BASE COUNT          489 a          822 c          932 g          491 t
ORIGIN      
        1 ccctcgacca ggcccgggat gcgcgtctgt tcatcctgga caagatggcc gaggccatct
       61 ccgagccccg ttccgagatg aatgagttcg ctccacgcat cgagtcggtc gagatcccca
      121 aggacaagat cggcgaggtc atcggtccca agggcgccgt gatccgggag ctggaggaag
      181 tgaccggagc cgccatcgag atcgaggaga ccgacggcaa gggcatcgtc aggatcgcat
      241 ccaacgacgg ctcagctctc gccgctgcca aggagagggt gatgcagatc gccttcccgc
      301 cggaggtcga gatgggtgcc gagtacgacg gcaaggtggt gaacatcacc aagttcggcg
      361 ccttcgtcaa catcctcccc gggcgcgacg ggctgcttca tatctcccgt ctcgacggtt
      421 cgaagcgggt cgagcgagtc gaggactacc tggtcgacgg tcaggagctg aaagtcaggg
      481 ttcgcgagat cgatcggggc aaggtcagcc tggagctggt cgaagccctg gaaggggcga
      541 cccttcctga gcccgaggcc ccgcgtcagg acggtggtga ccgtcgtcgt gacgacggcc
      601 gtgatcgtgg tggacgtggc ggaggtcggg accgtggcgg gcgcgatcgt gatcgtgacc
      661 gtggcagccg ggaccgcgac cacagcccca gtactgccga caccccatcc cagccggttg
      721 agaccgaggc ggggtcagag cggcggcgtg ccgcccagag cttcgacgag gtcttcgagg
      781 agatcagcga gcgctagttc gcgctaactc atacgtgcag aaagggacgg gcacagcccg
      841 tccctttctg gttcttagcg tgttggtcga ccggtcatag actcaggtcc accgttgctg
      901 gccaccggag gcaggtaatt ggcggttgag acgcgtggga ccgtgcagat gcgcggtgac
      961 tcgggcccag gagtcaaagt ggaggtgctg gccgacggct cccgcatcag ccttctccac
     1021 cgcgacgagc tgatcggtga atgggacgtg ggcgcgattg gcatccaatc gctcaacgac
     1081 gggttcgcca tccgggccga gggtgaggaa ttcgtgctga gaaccgagga cgacgtcggg
     1141 ctggcccgtg agatcggaat cgtcgccgct tcgccacgca tggcccgcaa ggtcgctgca
     1201 tctcacaatg atgatgcccc gttgagggcc gatcccgagc cggtcgtggt caagtccaac
     1261 ctgggagcga ttgcctttgc tctcggcggg gcgatggcgc tcgccggcgg tttcctcctc
     1321 cgcgacgatc ccacgttgtc ggccaacgcc acatccgcca gtcaagggtt cggggccgag
     1381 ggcagcttct ggttcgcgtt cacgctcggt ggtctcctca tggtgggcgt ggcgatggcg
     1441 atagccagga ggacgaaatg gtcgcgactg gcatcaatca tcgtcgtctc ggctctcgtt
     1501 gtcgtgttcg gcctcgccgc tcgaagcgcg acgcctgatg ccgaccactt gctcgcctac
     1561 ggcttcatcg ccgggggagt cgtggtcggc gtcgcggtcg ttttcgctgg ctcgttcgtg
     1621 gagccggact agtcgggacc cagctgtagc ttccaccccg tgccggagct gcccgacatc
     1681 gtggtctatc tggaagcctt ggagagccgc gtcctcggtg aggtggtgcg cggggtcagg
     1741 attgcctcac cgtcggtgtt gcgcacctac gaccctcctt atgacgcacc ggtgggtctc
     1801 gagatcgaga cgctgcaacg tgtcgggaag cggatcgtat tcgggatgga ggacgatctg
     1861 tatctcgtga tccatctgat ggtggcgggc cgtctccgct gggagacacc gaagaagccg
     1921 attccgaaga aggtcggtct ggccgccatc gacttcgatc cggggaccct gatcctcacc
     1981 gagcagggaa cccacaagcg ggccgggatc tggctgagtc ggggattcga gggtgtgtcc
     2041 gagctggatc gtggcggggt agagccgctc gaggccgatg ccgcagagtt cggcagcgcc
     2101 ctcaagaggg agaaccggac actgaagcgc gcccttaccg atccccgcat cttctccggc
     2161 atcggcaatt cctactcaga cgagatcttg tggcaagcgc ggatgtcacc cgtgaagcgg
     2221 acatctcaga tgaccgacga ggaggtcgag cagctgcggc aggccgtggt ccaagcgctc
     2281 accgaatgga ccaaccggct ccgcgacgag gtgggtgaga attggccggc caaggtgacc
     2341 gcgtttcgcg atgacatggc ggtccacggc aagtacggcc agccttgtcc gcgctgtggg
     2401 tcgaaggtgc agcgcatcgt ccacgccgac aacgagacca actactgcgc cacctgtcag
     2461 accggaggca agctcctcgc cgatcgctcc ctctcccgtc ttctcaagga cgactggccg
     2521 aagacgatcg aggagctcga agaggggtag gacggcttta catcgaacat atgttcgata
     2581 agatggatcg cccgagcgtg gagccggttt gggcctcaaa gaagtcattg cagacgttgt
     2641 cgacgccata cctctcgtcg atctcaccga tcgtcaggat gggtttctcc aactgaagcc
     2701 ggggcatgta accgagttgc tcgggatccc cggt
//
LOCUS       DASTXO010000407         2777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_540606, whole genome shotgun sequence.
ACCESSION   DASTXO010000407 DASTXO010000000
VERSION     DASTXO010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540606"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VFU96_08570"
     CDS             <1..478
                     /locus_tag="VFU96_08570"
                     /inference="COORDINATES: protein motif:HMM:NF015129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU5113361.1"
                     /translation="AEGPGYHPGRSALARLGGSVIGHVGELSPRATREFDMPGRVAIG
                     EVELEPLLARVDAVIATSPSAFPHVDFDLSFLLPAELAVGQLIGATSDAGADLVESAR
                     VFDVFRGPGVDEGSRAVAIRYRLRSADHTLTNEEVAPVRQSMISAAEQLGARLRGA"
     gene            480..1082
                     /locus_tag="VFU96_08575"
     CDS             480..1082
                     /locus_tag="VFU96_08575"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014350420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEU5113362.1"
                     /translation="MGLPEQLAHSPEEAAPRLLGSFLVSDVGGKHVRARITEVEAYKG
                     PDDPASHAFRGRTRRNGSMFEQPGTLYVYRSYGIHWCANVSAGPEGVGWGILFRGAEI
                     VDGLGVARSRRGRERDLANGPGKLTQALGVDHSHDGTFLFDGSAPIRLEPGPTPALVM
                     ATPRVGISQAIDRPWRFVAVSPATGGGKSPDLPDLLSPRQ"
     gene            1079..2266
                     /gene="tyrS"
                     /locus_tag="VFU96_08580"
     CDS             1079..2266
                     /gene="tyrS"
                     /locus_tag="VFU96_08580"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU5113363.1"
                     /translation="MTSDTPLEFLLRHTDQVVPGDDLTRKLELGRPLRIKLGLDPTAP
                     AVTLGWAVVLRKLRHFQELGHTAVLIVGDFTGQVGDPSDRSETRRRLTAEEVNGYAEN
                     VLGQFRRILLPENLEIRRNSEWLGTLDMAGLLELTSAATVAQMLERSDFAKRYDDGSP
                     ISVMEFLYPLLQGYDSVAVEADVELGGSDQLWNLMMGRTVQERYGLEPQVAMTMPLLV
                     GTDGVKKMSQSLGNYIGVEDPPDQMFGKTMSIPDTAMVEWFRLAADLDPSEVEEIEAG
                     LTSGSLHPGETKRRLARAVTALYWGDDGAASAESAFDQVFKEGGMPDEIDSFALPADD
                     PVAVPGLIRDALGLSGSEARRLLAQGAVKLNGSVLEAQEVSRDDLSGRVLQVGKRRFV
                     RLV"
     gene            2652..>2777
                     /locus_tag="VFU96_08585"
     rRNA            2652..>2777
                     /locus_tag="VFU96_08585"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          474 a          869 c          923 g          511 t
ORIGIN      
        1 ggctgagggt cccggctatc acccgggccg gtcggctttg gcgcgtctcg gcgggtcggt
       61 catcggtcat gtcggtgaac tgagcccacg cgccacccgg gaattcgaca tgccgggccg
      121 ggtcgcgatc ggggaggtcg agctcgaacc cttgctggct cgggtcgatg cggtgatcgc
      181 cacctctccg tcggcgttcc ctcacgtcga cttcgacctc tccttcctct tgccggcgga
      241 actcgctgtc ggtcagctga tcggcgccac ttccgatgcc ggcgccgatc tggtcgagtc
      301 ggcccgtgtc ttcgatgtgt tccgcgggcc cggagtcgac gagggcagcc gagcggtggc
      361 aatccgatac cgactgcgtt cggcagacca cacgctcacc aacgaagagg tggcgccggt
      421 ccgtcagagc atgatctcgg ctgccgagca actcggcgct cgtctgcgag gcgcctgaga
      481 tgggtctccc ggagcagctc gcacacagcc ccgaagaggc cgccccccgc ctgctgggct
      541 cgttcctggt ctcggacgtt ggcggcaagc atgtcagggc tcggataacg gaggtcgagg
      601 cttacaaggg ccccgacgac ccggcctccc atgcctttcg aggccggacg agacgcaacg
      661 gctcgatgtt cgagcaaccc gggacgctgt acgtctatcg ctcatatggc atccactggt
      721 gcgccaacgt gtcggcgggc cccgaaggcg tgggatgggg aatcttgttt cgcggagccg
      781 agatcgtcga tgggctcggt gtggctcgtt cgcggcgagg ccgggagcgg gatctcgcca
      841 acggcccagg gaagctgacc caggcactag gcgtcgatca ctctcacgat gggacgttct
      901 tgttcgacgg gtccgcgccg atccgattgg agccaggccc gacgccggcc ctggtgatgg
      961 cgacaccgcg agttggcatc agccaggcga tcgaccgccc gtggcgtttc gtggcggtgt
     1021 ccccggccac tggtgggggg aagtccccgg atcttcccga cctactctcg ccccgccaat
     1081 gacctctgac accccgctcg agttcctgct ccgtcacacc gatcaggtag tgccgggcga
     1141 tgacctgacc aggaaactgg agttgggacg cccgctccgg atcaagctcg gtctggaccc
     1201 caccgcccct gcagtgacct tgggctgggc cgtcgtcctc cgcaagctgc gtcatttcca
     1261 ggagctcggc cacaccgcgg tcctcatcgt cggcgatttc accggccagg tgggtgaccc
     1321 gtcggaccgg tcggagacac gccgaagact caccgcagag gaggtcaacg ggtacgcgga
     1381 gaacgtgctc ggccagttcc ggaggatcct gcttcccgag aacctcgaaa tcaggcgaaa
     1441 ctccgaatgg ctggggacgc tggacatggc cggtctgctc gagctcacct cggcggccac
     1501 ggtggcccag atgctggaga ggtcagactt cgccaagcgc tatgacgacg ggagccccat
     1561 ctccgtcatg gagtttctct atccactcct ccagggctac gactcggttg cggtcgaggc
     1621 cgacgtcgag ctgggagggt cggaccaact ctggaacctg atgatggggc ggaccgttca
     1681 ggagcgatac ggcctggagc cccaggtggc gatgacgatg ccgctcctgg tcggcacgga
     1741 cggagtcaag aagatgtccc agtcgctcgg caactacatc ggtgtcgagg atccgcctga
     1801 ccagatgttt ggcaagacga tgagtatccc ggacaccgca atggtcgagt ggtttcgttt
     1861 ggctgcggat ctcgacccgt ccgaggtcga ggagatcgaa gccggtttga cttcgggctc
     1921 gcttcacccg ggtgagacca agcgacggct ggcacgtgcc gtcacggctc tgtattgggg
     1981 tgacgacggg gccgcgagcg ccgaatctgc gttcgatcag gtgttcaaag agggtggaat
     2041 gcctgacgag atcgattcgt ttgcactacc ggcagacgat ccggtggcgg tcccgggact
     2101 gatcagggac gccctcgggc tgtcgggctc ggaagcccgg agattgttgg cccaaggagc
     2161 ggtgaagctc aacgggtcgg tcctggaggc ccaggaggtc tccagggatg atctctccgg
     2221 ccgggtgctc caggtgggca aacggcgctt tgtccggctc gtctgattga cttgactaac
     2281 tcaggggtgt cgttactatt cgactcccgg ctggggaaca catccccggc cacccacttg
     2341 aacaccggga cggcgcctct ttcgcgtcgc ctcggattca ctttgtatcg gggccacgat
     2401 ggcatccgcg actggatcgc agttgtctcg tcggcccgcc tgacatcagt agtgggagct
     2461 ctgggctcct tgacaactga acaatgtgcc aaaagccagc atgcacaccc actctcgtgt
     2521 cttcggactg acgagtgagg tgagcatctg tatagcaatc agctctaggg cccctcacgg
     2581 gccctggacc caattgcaaa ctcataactg acaggattgg ccggcgccca cgggtgccat
     2641 gccttgaatg tacgttggag agtttgatcc tggctcagga cgaacgctgg cggcgtgcct
     2701 aatgcatgca agtcgagcga cgcacaccgg aggggtaacc caccggtatg acggagcggc
     2761 gaacgggtga gtaacac
//
LOCUS       DASTXO010000408         2001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1608245, whole genome shotgun sequence.
ACCESSION   DASTXO010000408 DASTXO010000000
VERSION     DASTXO010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608245"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VFU96_08590"
     CDS             complement(<1..337)
                     /locus_tag="VFU96_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113364.1"
                     /translation="MSRSRLVVLIVLLIAIVAIVAICNRASDPPTTTDLTTSTLAVTT
                     SASVDPTTTTTSEPAPTTTAGAPTTSPPAEQTATVLLSPFSDMGPAWTEQIFPYGEGE
                     EFLGTSPGGD"
     gene            415..>2001
                     /locus_tag="VFU96_08595"
     CDS             415..>2001
                     /locus_tag="VFU96_08595"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEU5113365.1"
                     /translation="MSAIRFGISRLPPDDVADEEFLDDLVAKGHRALELPFVDGFPWK
                     EKRCEAFGALAVERDLRLSVHAPYFAGLTLPDEDRGRQSLAALEHTMKLGKALSAPVI
                     VAHFGSNYSEEPGVLMDRIRSRLDAVVAKVETLGVGMGLETAGNNRSFGSLGDIAMLA
                     SEYSFVRPVVDWAHVHAMTGGGLTSKEAFVSVFSFIQSQFPGWMIAPLQTQFSDNEFG
                     AGGEIRHIAYGEGTLRVGPLVEAAQEAGVGMVLISEARDLASHDRIWEEAREQMESGP
                     SAAEGRPIFATESPYPTPVTAIKDGDGFRPVGLDRSLRLTNLDKVYFPDDGYTKGDLI
                     QYYASVAPALLPHLEGRPISMSRYPEGIKGPSFYEKRAPGHQPDWMRTTPVVSDSQGG
                     VIDFLVADTVEALMWFANMGCIETHPFHSREGRLEHPDYAIFDFDPAEGSTWDQVVEG
                     GRLVKVALSQLGLTGYPKLSGSRGLHVYVPLEPVHRFSRVRRFVGEVGNYLAAANPSA
                     LTMEWDKPKRKGKVFIDHNRN"
BASE COUNT          341 a          588 c          693 g          379 t
ORIGIN      
        1 cgtcgccgcc cggcgacgtg ccgaggaact cctctccttc accgtaggga aagatctgtt
       61 cggtccacgc cgggcccatg tctgaaaacg gggagaggag gacggttgct gtctgctctg
      121 cagggggact ggtggtggga gcgccggccg tagttgtcgg cgcaggttcc gacgttgtcg
      181 ttgtcgtggg atcgactgat gcagaggtgg tgacagcaag ggtcgaggtc gtgaggtcgg
      241 tcgtcgtcgg tggatcggaa gctcgattgc agatggcgac gatggcgacg atggcgatga
      301 ggagcactat caagaccacg agccggctgc gagacatggg gtcaggttat gacgagggtg
      361 tccaccggag taggactcca agacaaaggc ggtcggctcg atagcctcgc gctggtgagc
      421 gccatccgct ttgggatctc acggttgcct ccggacgatg tcgcagacga ggagttcctc
      481 gatgacctgg tggcgaaagg ccatcgtgcc ttggagctgc cgttcgtcga tggtttcccc
      541 tggaaggaga agcgttgtga ggccttcggg gcgctggccg tcgagcgtga cctccgtctt
      601 tcggtccatg cgccgtactt cgccggcctg acattgcccg acgaggaccg gggccggcaa
      661 tcgctggccg ccctggagca caccatgaag ttggggaagg cgctgtcagc gcccgtgatc
      721 gtggcgcatt tcgggtcgaa ttactccgaa gagcctggcg tgctgatgga tcgaatccgg
      781 tcccggctcg atgcggtcgt cgccaaggtc gagacgctcg gggtggggat gggtttggag
      841 acggccggca acaatcgatc gttcgggagc ctcggcgaca tcgccatgct cgcctccgag
      901 tactcctttg ttcgccccgt cgtcgactgg gcccatgtcc acgcaatgac cggcggcggt
      961 ctcacgtcga aagaagcctt tgtctcggtc ttctccttca tccagagtca attccccggc
     1021 tggatgatcg cgccgctgca gacccagttc tcggacaacg agttcggtgc cgggggtgag
     1081 atacggcaca tcgcctatgg ggagggcacc ctccgtgtcg gcccgctcgt cgaggcggca
     1141 caggaggcgg gagtcggcat ggtcctgatc tcggaggcca gggacctggc cagtcacgac
     1201 cgcatctggg aggaggcccg cgagcaaatg gaatccgggc cttcggcggc ggagggccgg
     1261 cccatcttcg cgaccgagtc cccgtacccc accccggtca ccgcgatcaa ggatggagat
     1321 ggctttcggc cggtgggact cgatcgctcc cttcgcctga cgaatctgga caaggtctac
     1381 ttcccggatg acggctacac gaaaggtgac ctgatccagt actacgcctc ggtggcgccc
     1441 gcgctgcttc cccatctgga agggcgcccg atctcgatga gtcgatatcc ggagggcatc
     1501 aagggcccat cgttctatga gaagcgggcg cccggccatc agccggactg gatgaggacg
     1561 acgcctgtcg tgtcggactc acagggcggt gtgatcgatt ttctcgtcgc cgacaccgtg
     1621 gaggccttga tgtggttcgc caacatgggg tgcatcgaga cgcatccttt ccactcccgt
     1681 gaagggcgac tggagcaccc cgactacgcc atcttcgact tcgatccggc cgaaggctcc
     1741 acgtgggacc aggtggtgga gggaggtcgg ctggtgaagg tggcgttgag ccagctcggg
     1801 ctcaccgggt accccaagct gtccgggtcg agagggcttc atgtctacgt cccgctggaa
     1861 ccggtccacc ggttctcgag ggtgcggcgt ttcgtcggtg aggtgggcaa ttacctggcc
     1921 gcggccaatc cgagtgccct caccatggag tgggacaagc cgaagcggaa gggcaaggtc
     1981 ttcatcgatc acaatcgcaa c
//
LOCUS       DASTXO010000409         2262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_430044, whole genome shotgun sequence.
ACCESSION   DASTXO010000409 DASTXO010000000
VERSION     DASTXO010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430044"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..690)
                     /locus_tag="VFU96_08600"
     CDS             complement(97..690)
                     /locus_tag="VFU96_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113366.1"
                     /translation="MSNRGAEWELEMEESGEGVDGFRAAGEQLHLEKARNQAEKPSST
                     PKRAESRDPTRARRQRARVRRIELREAMERLEATVARPAIPDGWSDAVDASLRQLQEA
                     LEAHIREVEAPDGLLADIVDMAPRLVSEVETIKDEHRELARSGARARETLRGHGVASV
                     TEVRRRVMSFLGRLAIHRQRGSDLVYEAYNDDIGAAG"
     gene            complement(815..>2262)
                     /locus_tag="VFU96_08605"
     CDS             complement(815..>2262)
                     /locus_tag="VFU96_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017681091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEU5113367.1"
                     /translation="QILNLHVFHLLQTVSEHSSALDVGVPARGLHGEAYRGHIFWDEL
                     FIFPFLNYRYPELTRALLLYRYHRLPEARSAARDEGHRGAMYPWQSGSNGREESQLVH
                     LNPESGRWIPDNSRLQRHIGLAVAYNVWQYFQATGDIDFLVVYGAEMLVEIARFWASL
                     AGYDHQSGRYRILGVMGPDEYHDAYPGAGQPGLDDNAYTNVMAVWTLLRAREALDLLR
                     EDRSRRLRDRLGLREEELEEWESITRKMRIPFHDQGIISQFDGYEQLEEFDWEGYRGR
                     YDDIQRLDRVLEAEGDTPNRYKASKQADVLMLFYLLAAEELQEIFARLDYPWDRDIIP
                     RNVAYYRARTSHGSTLSWVVHAWVLSRSNRPESWDLFHAALESDVGDIQGGTTPEGIH
                     LGAMAGTIDLIQRCYAGLEPRGDVLCFNPDLPEQLESIEFRLIYQGRWLDVRIEGRNV
                     AVTAESDGDSPVAVEVGGRVCLLAPGETAQG"
BASE COUNT          383 a          723 c          723 g          433 t
ORIGIN      
        1 cgtgagacga ttgattggat cgaatcgggt ctctgatcag agatcgatgc cagaggttcg
       61 ctcgaggatg gggggtgtga tcggagttgg tcgggtctag ccggcggcgc cgatatcgtc
      121 gttgtaagcc tcgtatacga gatcagaccc tcgttgccgg tggatcgcca gacgaccaag
      181 gaaagacatg acccgtcggc ggacctctgt cacggacgcc acgccgtggc cacgcaaggt
      241 ctcccgagct cgggccccgg atctcgccaa ttcccgatgt tcgtctttga tcgtctcgac
      301 ttcggatacg agtcggggag ccatgtcgac gatgtctgcc agcaggccat cgggtgcctc
      361 gacttcccgg atatgggctt cgagtgcctc ttggagctgt ctcaaggagg catcgacggc
      421 atcggaccaa ccatcgggta tcgctggtct ggcgacagtg gcctccaggc gctccatcgc
      481 ttcgcggagc tcaatgcgac ggacccgtgc cctttgccgg cgagctcggg tcggatcccg
      541 cgactctgct ctcttggggg tcgaactcgg tttctccgcc tggttgcggg ctttctcgag
      601 atggagctgt tcgccggccg cgcgaaaacc atcgactcct tcgcccgact cctccatctc
      661 cagctcccat tccgcgccgc ggttgctcat tgacctcatc atgcaggggc ttcgtcggtg
      721 aagtaagggc caaagggccc gacctcgcaa cgccgacgac aggtggcgcg ggccggaatc
      781 cgttcctgac tggtcttggt tgaggcgccc ctattcagcc ttgtgccgtc tccccagggg
      841 cgagcaagca gacccggccc ccaacctcca cggcgaccgg agagtccccg tcggactcgg
      901 cggtgaccgc cacgttccgc ccctcgatcc ggacgtcgag ccaccggccc tggtagatga
      961 gccggaactc gatcgactcg agctgctcgg gcaggtcggg gttgaagcag agaacgtcac
     1021 ccctgggctc gagaccggca taacagcgct ggatcagatc gattgttccg gccattgccc
     1081 cgagatggat tccttccggt gtggtcccgc cctggatgtc gcccacgtcg gattccaagg
     1141 ccgcgtggaa caggtcccat gactcgggac ggttggaacg tgacagcacc caggcgtgga
     1201 cgacccagga cagggtcgaa ccatgagagg tccgcgcccg gtagtaggcc acattccgtg
     1261 gaatgatgtc tcggtcccac gggtagtcga gtcgagcgaa gatctcctgc agctcctcgg
     1321 ccgcaaggag gtagaacaac atcaggacgt ctgcctgctt cgaggccttg tagcggttcg
     1381 gagtgtcgcc ttcggcctcg agaacccggt ccaggcgctg aatgtcgtcg taacgcccgc
     1441 ggtacccctc ccagtcgaac tcttccagtt gctcgtagcc gtcgaactgg ctgatgatcc
     1501 cctggtcgtg gaacgggatt ctcatcttgc gggtgatgct ctcccattcc tccaactcct
     1561 cctcgcggag cccgagtcgg tcccgaagtc gacggctacg gtcttcgcga aggagatcga
     1621 gcgcctcccg tgctctcagc agggtccaga ccgccatcac gttggtgtag gcgttgtcgt
     1681 ccagacccgg ctgtccggca cccgggtagg cgtcgtggta ctcgtcgggc cccatcaccc
     1741 ccaggatgcg gtagcggccg ctctgatggt cgtatccggc caggctggcc cagaagcggg
     1801 cgatctcgac cagcatctcc gctccataga cgacgaggaa atcgatgtcg ccggtggcct
     1861 ggaagtactg ccatacgttg taggccaccg cgagcccgat gtgacgctgc agtcgtgagt
     1921 tgtccgggat ccacctcccc gattccggat tgagatggac cagctgactc tcctcccgac
     1981 cgttgctgcc gctctgccat ggatacatcg caccccggtg gccctcgtcg cgggcggccg
     2041 agcgcgcctc ggggagacgg tgataccggt agaggagcaa cgcccgggtc aactccgggt
     2101 agcgatagtt gaggaagggg aagatgaaca gttcgtccca gaagatgtgt ccccggtagg
     2161 cctctccgtg gaggccacgg gccggaaccc cgacgtcgag agcgctgctg tgctccgaca
     2221 ccgtctgcag gaggtggaag acgtgcaggt tgaggatctg cc
//
LOCUS       DASTXO010000410         2434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_319531, whole genome shotgun sequence.
ACCESSION   DASTXO010000410 DASTXO010000000
VERSION     DASTXO010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319531"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..1042)
                     /locus_tag="VFU96_08610"
                     /pseudo
     CDS             complement(98..1042)
                     /locus_tag="VFU96_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018787508.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
     gene            1141..2037
                     /locus_tag="VFU96_08615"
     CDS             1141..2037
                     /locus_tag="VFU96_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEU5113368.1"
                     /translation="MTRIARYAWFTLVFNVGVILLGALVRATGSGAGCGKSWPTCQGE
                     VVPRLSGATAVEFTHRAASGVALVLVAVLVVLVWRRVPSGEPARTGAGLSLAAIVGEA
                     LIGAMIVLAEWVAEDASLARVVAVPLHLVNTLFLLAGLSLTAFWLSGGRRLSTKSHPR
                     IWRWVVLGGLAIVLLAATGAVTALADTLFPKAASSQTEPGSAHFLTDLRIIHPVLAVL
                     AASIGWWASGRLGVAPRSRAAQALPLLVGLMLLSGALNVILGVPVWMQLFHLLLADAL
                     WIAYVLASAQALQVPAEVGQPV"
     gene            2037..>2434
                     /locus_tag="VFU96_08620"
     CDS             2037..>2434
                     /locus_tag="VFU96_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5113369.1"
                     /translation="MFDQLADGVFRRRYESLDLNVGVVLGEDGALIIDTRASHVEADE
                     LLDELGTLTSLPVRWVIDTHWHWDHTFGNARFPAAEIWGHERCRDVMLDRADSMKEDA
                     ASWLPDRLAEFAEVVITPPTRFFDTLARIDL"
BASE COUNT          358 a          801 c          787 g          488 t
ORIGIN      
        1 gtccattcgt ggcggcgtct cacgccgaag caaggccaca gctgatattc gaacgaaagg
       61 cccccgatcc atcgggggcc tttcgctgtc aggttgcgaa gcctttgtcg gccatccagt
      121 cgtcgtcgaa gatcttcgac aggtagcccc gccctgaatc gggcaggacc acaaccacca
      181 tcgcatcggg attctctacc gccacctgga gagcaccatg ggcggccatg ccacaggaac
      241 cccccaccag gatgccctct tctctggcca gccttctcgt catggtgaat gagtcggcgt
      301 cattcacctt gatccactga tcgattaggt cgagctccac agtctccggg tagaagtcct
      361 caccgacccc ctccaccgcg tactgatgaa cctcggcgtc gctgtcggcg gtgaagatgg
      421 agccctcggg gtccacgccg atgacccgca ccgacggctt ttgttccttc aagtagcggc
      481 ctgcgccgct gatggtgcct ccggtgccga ccccgacaac gagcgcatcc accgaacctt
      541 ccgtctgccg ccagatctca gggcccgtcg tgtcgtagtg ggcctgggga ttcgcccggt
      601 tcgaatactg gtccgggtgg aaagcccctt cgatttcctc cgtcaaccgt cgcgccaccc
      661 cgtaatagga gtccgggtgc tcggggagga ggttggttgg ggtcaccacg acctcgacgc
      721 cgtaggcgcg gagcaggtct tgcttctcct tggagacctt gtcggggacg gtgcagatga
      781 cccgatatcc ccgaactgcc ccgacaagag cgagccccac accggtgttc cccgaggtcg
      841 gctcgacgat cgtgccccct ggccggagga gcccctgtgt ttcggcgtcg tcgatcatcg
      901 ccaccgcgat tcgctccttg accgacccgc ccgggttcat gtgctcgact ttggccacga
      961 ggttgcccgg aggatggatc cgggcgagcc tgatcatcgg tgtgttgccg atcgcggcga
     1021 ggacgtcggg atggatctcc atgggcggag cgagggtatc gcaccggatg gtgcacctcc
     1081 cgaacgactg actttccgaa gcggcgaagc cgggaagagg gaccttctaa tctttccgcg
     1141 gtgacccgaa tcgcccgcta cgcgtggttc accctcgttt tcaacgtcgg ggtgatactg
     1201 ctcggggcat tggtccgggc caccgggtcc ggggctggat gcggaaagtc gtggccgacg
     1261 tgccagggcg aagtagtgcc ccggttgtcc ggcgcgaccg ccgtcgagtt cacccatagg
     1321 gcggcgagtg gtgtggccct ggtcctggtg gcggttctgg tcgtgttggt ctggcgtcga
     1381 gttccctcgg gagagccggc taggacgggt gccgggctgt cactggccgc gatcgtgggg
     1441 gaggccctca tcggggcgat gatcgtgctc gccgagtggg tcgcggagga cgcatctttg
     1501 gcccgggtcg tcgcggtacc gcttcatctc gtgaacacgc tgttccttct cgccgggctg
     1561 tcactgacgg ctttctggct gtccggagga cgacgtctgt cgaccaaatc ccatccgaga
     1621 atttggcgtt gggtggtgct cggggggttg gcaatcgtcc tgctggccgc caccggcgcg
     1681 gtgaccgccc tcgccgatac cctgtttccc aaggcagctt cgagccagac ggagcctgga
     1741 agcgcccatt tcctcaccga ccttcgcatc atccatccgg tcctggcggt gctcgccgct
     1801 tctatcggat ggtgggcttc agggcgtctt ggcgtggcgc cgcgttcgag ggccgcccag
     1861 gccctgcctc tgttggtcgg gctgatgctc ctgtcgggcg cgctcaacgt gatcctcggc
     1921 gtcccggtgt ggatgcagct gttccatctg ctgctcgcgg acgccctgtg gattgcctac
     1981 gtgctcgcct cggcccaggc cttgcaggtg cccgccgagg tcggccaacc ggtctgatgt
     2041 tcgaccagct cgctgacggc gtgttccgcc gacgttatga atcgctcgat ctcaacgtgg
     2101 gcgtggtcct cggagaggac ggcgccttga tcatcgacac ccgcgcctct cacgtcgagg
     2161 ccgacgagct cctcgacgag ctgggcacgc tcacctcgct gccggtgcga tgggtcatcg
     2221 acacccattg gcactgggac cacacctttg gcaacgcccg cttccccgcg gcagagatct
     2281 ggggacatga gcgctgccgg gacgtcatgc tcgatcgggc cgattcgatg aaggaggacg
     2341 cggcgtcgtg gcttcccgac cggctggcag agttcgccga agtggtcatc acccctccga
     2401 ccaggttctt cgacactctt gcccgaatcg acct
//
LOCUS       DASTXO010000411         3561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1351023, whole genome shotgun sequence.
ACCESSION   DASTXO010000411 DASTXO010000000
VERSION     DASTXO010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351023"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VFU96_08625"
     CDS             complement(<1..643)
                     /locus_tag="VFU96_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007730570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5113370.1"
                     /translation="MTDRTKSAAGIVLLTLASGQFLMTLDSSVMNVSIAQVAEDVGTT
                     VTGIQTAITLYTLVMATLMITGGKIGSLIGRRRAFMIGCVIYGAGSLTTSLAPTLTVL
                     IIGWSFLEGIGAALIMPAIVALVASNFPPEGRPRAYGLVASAGAIAVAAGPLIGGFVT
                     TNWTWRYVFAGEVVVVAAILLLTRRMRDAPPEAKHRLDLVGAALTATGLGLAVF"
     gene            817..1965
                     /locus_tag="VFU96_08630"
     CDS             817..1965
                     /locus_tag="VFU96_08630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5113371.1"
                     /translation="MSRGDGITANASGSGMLAEAVDGDRLVQTFLDLVAIDSPTGHEE
                     EIGKELSARFTTLGCTVTIDDVGNLVAVLPGTRPDTVLLSTHMDTAGTDIGIQPIIEE
                     GVIKTDGTTILGADDKSGIAGCLELLTILRSYPEVEHPTLEFVISVGEESGLLGSRQM
                     DLNRLNSTYGFVFDTAGDIGAITYWTPTSVYITVTIRGKKAHAGVEPEKGINAVKVAA
                     EAIATMPLGRIDEETVANIGTITGGEARNIVPGEVTMQGMARSHDQAKLDAQLEAMRS
                     SLAEAADRHGATLDFEAEEIYRTYHIDESERPYREAARAIRSLGLEVIPRKSGGGTDG
                     NFFNAHGIACVAMATGMVDEHAATEHIAIEDMVMACKILVTIVTQEPE"
     gene            1979..2398
                     /locus_tag="VFU96_08635"
     CDS             1979..2398
                     /locus_tag="VFU96_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113372.1"
                     /translation="MTGFRRRRWVIAMTALALTIVACGNAQERDLCRQYEDLQEAVAQ
                     VESLDPATATAADVVGIVDEVIVQLEQFQAAATEGNYELAVSNLTFALTELRQVAFDL
                     GEEGLEVARPLMQESVETSVAAYNALQERLDVVCGTD"
     gene            2414..>3561
                     /locus_tag="VFU96_08640"
     CDS             2414..>3561
                     /locus_tag="VFU96_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003940851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter family
                     protein"
                     /protein_id="HEU5113373.1"
                     /translation="MDITFILEAVVVLAAIVMGTRAGGVGIGVWGGLGVAVLVFVFGE
                     APGEPPISALLIILAVVTAAALMQVAGGVDWMVKIAAAVIQRHPKQITLIAPLTAFLF
                     TVGAGTSNILYPLLPVIYDVSYQNGIRPSRPLTVTVVQSGMSLAASPVSAAMAAMVTL
                     TAVEPYNFDLAQILSITFPAGVIGIIVTALIVGRMGKDIDEDPEIQERIASGALGQPG
                     SAGQTVELTSTKEGRNAGIIFLLGVLAIVILGLFPDLRPAFEVEGEAVTIDMTTTIEL
                     VMFTVGVLIIMVSKPDVSEVPGASVFKAGMVSAIALFGIAWLTATFIAAHEEFIVTTI
                     GGWVNQAVWIFAIAVFLVAALTTSQSTATRTIVPIGLAAGLAPGLVTG"
BASE COUNT          676 a         1118 c         1147 g          620 t
ORIGIN      
        1 cgaagacagc cagaccgagc ccggtcgcgg tgagggcagc accgaccagg tccaaccggt
       61 gtttggcttc agggggcgcg tctcgcatcc ttcgagtcag caggaggatg gcggccacca
      121 ctacgacctc gccggcgaac acatatctcc aggtccagtt cgtcgtgacg aagccgccga
      181 tcaggggccc cgctgccact gcgatggcgc cggccgaggc cacgaggccg taggcgcggg
      241 gcctcccctc aggagggaag ttcgaagcga ccagcgcgac gatcgccggc atgatcagcg
      301 ccgcaccgat cccctccagg aacgaccagc cgatgatgag cacggtgagg gtgggagcca
      361 acgaggtcgt cagcgagcca gcgccataga tgacgcagcc gatcatgaac gcccgcctgc
      421 gaccgatgag gctgccgatc ttcccgcccg tgatcatcag cgtcgccatg accaatgtgt
      481 agagcgttat cgccgtctgg atgccagtga cggtcgtgcc cacatcttcg gcgacctggg
      541 caatcgagac attcatgacc gagctgtcga gggtcatgag aaactgaccc gacgccagcg
      601 tcagaaggac tatcccggcc gcgctcttgg tcctgtccgt catctcgtcg cctcgaccga
      661 cgtgggtgcg gcgaggggca gcggatccga gagtgggcaa accagcaccg gcgtcacgat
      721 acggatccgg tttgcatctg aggtgttttc gacatcggtc gagctcatcc cgaatgccgg
      781 tcccggcgac gcgcctcgaa gatatagtgc cgcccgatga gtcggggtga cggcatcaca
      841 gccaacgcga gtggatccgg catgctcgcc gaagcggtcg acggggatcg actcgtgcag
      901 acatttctcg atctcgtggc gatcgacagc cccaccgggc acgaagagga gatcggcaag
      961 gagctatctg cgagattcac gacgctcggt tgcaccgtca ccatcgatga cgtcgggaac
     1021 ctggtggccg tcctccccgg gacccggccc gacaccgtgc tgctctcgac gcacatggac
     1081 acggcgggaa ccgacattgg catccaaccg atcatcgaag aaggtgtgat caagaccgac
     1141 gggaccacca tcctcggcgc cgatgacaag tcgggtatcg cgggatgtct ggagctgctg
     1201 accatcctgc gctcctatcc ggaggtagag cacccgactc tcgagttcgt gatctccgtc
     1261 ggcgaggaga gtgggttgct tggatcccgt caaatggacc tcaaccgcct gaactcgaca
     1321 tacggtttcg tgttcgacac cgcgggcgac atcggcgcca tcacctactg gacgccaact
     1381 tctgtctaca tcaccgtgac gatccgcggc aaaaaggccc acgccggtgt ggagccggag
     1441 aaggggatca atgccgtgaa ggtggcggcc gaagccattg ccacgatgcc actgggccgt
     1501 atcgacgaag aaactgtggc caacatcggg accatcaccg gcggtgaggc ccgcaacatc
     1561 gtgcccggcg aggtcaccat gcagggcatg gcccgaagcc acgatcaggc gaagctcgac
     1621 gcgcagctgg aagccatgcg ctcctccctc gccgaggctg cggaccgtca cggggcgacc
     1681 ctcgatttcg aggccgagga gatctaccgg acttatcaca tcgatgaatc cgagcgacct
     1741 taccgcgagg cggcccgggc gatcaggtcg ctggggctcg aggtgattcc gcgaaagagc
     1801 ggaggcggga cagatggcaa cttcttcaat gcccacggaa tagcctgtgt cgccatggcc
     1861 accggcatgg tcgacgagca cgctgccact gagcacattg ccatcgagga catggtcatg
     1921 gcgtgcaaga tcctcgtgac cattgtcacc caggaaccgg aatgaaggga gcgccatgat
     1981 gaccggattc cgccgacggc gatgggtgat tgccatgacg gcgctcgccc tcacgatcgt
     2041 ggcgtgcggc aacgcgcagg agcgggatct ttgtcggcaa tacgaggacc tccaggaagc
     2101 cgtagcacag gtcgagagct tggacccggc gacggccacc gccgccgacg tggtggggat
     2161 tgtcgacgag gtgatcgtcc agttggagca gttccaggcc gccgccacgg agggcaacta
     2221 cgaactggcg gtttccaacc tcactttcgc cctgaccgag ttgagacagg tggccttcga
     2281 cctcggtgaa gagggcctcg aagtggcgag gccactcatg caggaatcgg tggagacatc
     2341 ggtcgccgcc tataacgccc ttcaagaacg actcgacgtc gtctgcggca cggactgacc
     2401 cgaaaggaga gaaatggaca tcactttcat cctcgaggcg gtggtggttc ttgccgccat
     2461 cgtcatgggc acccgtgccg gaggagtagg gatcggcgtt tggggcggtc ttggagtagc
     2521 cgtcctggtc ttcgtcttcg gcgaggcccc cggagagccg ccgatctccg ccctgttgat
     2581 catcctcgcc gtggtcaccg cggcagccct gatgcaggtg gccggcgggg tcgactggat
     2641 ggtcaagatc gccgccgctg tcatccagcg gcaccccaag cagatcacgc tgatcgcgcc
     2701 attgaccgcg tttctcttca cggtgggggc ggggacgagc aacatcctct atccgctgtt
     2761 gcctgtgatc tacgacgtgt cctatcagaa cggcatccgg ccctcacgtc ccctgacggt
     2821 cacggtcgtg caatccggga tgtcgctcgc cgcttcgccg gtctcggcag cgatggcggc
     2881 gatggtgact ctgactgcgg tcgagcctta caacttcgac cttgcgcaga tcctgtcgat
     2941 cactttcccc gccggtgtca tcggcatcat cgtcaccgct ctgatcgtgg ggcggatggg
     3001 caaggacatc gacgaggatc cggagatcca agagagaatc gcctccggag cgctgggaca
     3061 gccggggtcg gcagggcaga cagtcgagtt gacttccacc aaggaggggc gcaacgcggg
     3121 gatcatcttc ctcctcggtg tgctcgccat cgtgatcctg ggtctgttcc ctgacctgcg
     3181 gcccgcgttc gaggtggagg gggaggcggt caccatcgac atgaccacca cgatcgaact
     3241 ggtcatgttc acggttggtg tcctcatcat catggtgtcg aagccagacg tcagcgaggt
     3301 gcccggagct tcggtgttca aggcgggaat ggtctcggca atcgccctgt tcggcatcgc
     3361 ctggctcacc gccaccttca tcgccgccca cgaggaattc atcgtcacca cgatcggtgg
     3421 atgggtcaat caggcggtgt ggatcttcgc aatcgcagtg ttcctggtgg ccgccttgac
     3481 cacgagtcaa tcgaccgcga ccaggaccat cgtcccgatc ggcctcgctg caggattggc
     3541 gcctggcctg gtcaccggca t
//
LOCUS       DASTXO010000412         2182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_945718, whole genome shotgun sequence.
ACCESSION   DASTXO010000412 DASTXO010000000
VERSION     DASTXO010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945718"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..261
                     /locus_tag="VFU96_08645"
     tRNA            187..261
                     /locus_tag="VFU96_08645"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:222..224,aa:Asp,seq:gtc)
     gene            292..364
                     /locus_tag="VFU96_08650"
     tRNA            292..364
                     /locus_tag="VFU96_08650"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:325..327,aa:Phe,seq:gaa)
     gene            467..976
                     /locus_tag="VFU96_08655"
     CDS             467..976
                     /locus_tag="VFU96_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016430414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEU5113374.1"
                     /translation="MQSSDLAEILKHPVSQEMLERDLTRLAYVATDGTPRVVPIGFTW
                     NGTEVVMCTPTNAPKLVSLRQNPAVALTIDTEVHPPHILLIRGEAVLDEVEGIPEEYL
                     QASGSYRMTPEQRVEWEAEIRSLYDSMVRIVVEPTWAKLIDFEQTLPTPVEELIRRRE
                     ERQHSQPVQ"
     gene            complement(1015..>2182)
                     /locus_tag="VFU96_08660"
     CDS             complement(1015..>2182)
                     /locus_tag="VFU96_08660"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HEU5113375.1"
                     /translation="RNHEQTQPNPVGAFGDVSKAYDPAAPGGTTTLEISPTADEVSSW
                     VSLNGTTFNCAGGASPWGTWLTCEETPNGIDQQRSFLIGPNDPDDLTYTQKHGYLFEV
                     PVSRGPGELEIADPIVSAGRFAHEAAAVDPATGDVYMTEDDFAYASGFYRYRPPNRPQ
                     QDKRIADGGALHILGVIPPGASEPVTVDLHAAQVPGTTYQVGWIEIEDPDPTFAPGLS
                     NDEAARVVYQEGESKGAARFSRLEGMDYFDGKIFFVSTEGGGPFDSDPPPNASAGFGG
                     GYGQVWMYDISKETLTLVFESPGPDVLDFPDNITISPRRKSVILCEDSSAGNMLRALT
                     RDGLIFDFALNNVANGVDEFSGATFGPGGQVLYTNMQANGITFAIWGPWSNGLI"
BASE COUNT          413 a          676 c          707 g          386 t
ORIGIN      
        1 gtactggatc gggttcgcgc tgccgattgc gttgatcctg ggggccatcc gcacggtgct
       61 ggtgatcgtc gcactaccgg ggccatgatg agccgagttc cggggcccac cagatgttga
      121 gcagacaacg gtgagatcta tacttccccg gtcgccccga acctcgtcgg aatcggcgac
      181 gtccccgggg gcgtagaaca gcctggagtg ttcgccggcc tgtcaagccg gaggtcgcgg
      241 gttcaaatcc cgtcgctccc gccgaccgaa tccatttagg gttgggttgg aggtcaggta
      301 gctcagtcgg tagagcgccg gtctgaaaaa ccggaggtcg acggatcgac gccgtccctg
      361 accactttcc aaatcggatg cgcgatcgcc cgggtcatac cgcgtcaaca tcgaggcaaa
      421 gcccatgcat gcgtttgatc ggtccggaag cgggtaggag tatccgatgc agtcgagcga
      481 cctcgccgag atcctgaagc atcccgtcag ccaggagatg ctcgagcggg atctaacacg
      541 attggcctac gtggcaacgg acggcacccc gagggtcgtg ccgatcgggt tcacctggaa
      601 cgggacagag gtggtcatgt gcacgccgac gaatgccccg aagctggtgt cactgcgtca
      661 aaacccggcg gtagccctca ccatcgacac cgaggtgcac ccaccgcaca tcctcctcat
      721 ccgaggggag gccgtgctcg acgaggtcga ggggattccc gaggagtatc tccaggcgag
      781 cggcagctac aggatgacgc cagagcagcg agttgagtgg gaggcagaga tccggtcgct
      841 gtacgactcg atggtccgga tcgtcgtcga gccgacatgg gcgaagctca tcgacttcga
      901 gcaaacactg ccgacgcctg tcgaggagct catccgccgg cgtgaggaac gccagcactc
      961 ccagcccgtt cagtaagagg tcgactgatc gacggccacc atgcgtgacg ccggctagat
     1021 cagtccgttg ctccaaggcc cccagatggc gaacgtgatc ccgttggcct gcatgttggt
     1081 atagagcacc tgacccccag ggccaaaggt cgctccagag aactcgtcga ccccgttggc
     1141 cacattgttg agggcgaagt cgaagatgag cccgtctcgc gtcagggccc ggagcatgtt
     1201 gcccgcagag ctgtcctcgc agaggatcac actcttgcga cgaggcgaga tggtgatgtt
     1261 gtcggggaaa tcgagcacat ccggccccgg cgactcgaag acaagggtca gggtctcctt
     1321 ggagatgtcg tacatccaga cctggccgta cccgccaccg aagccggcgc tcgcgttagg
     1381 aggtgggtcg gagtcaaagg ggccgcctcc ctcggtggag acaaagaaga tcttcccgtc
     1441 gaagtaatcc atcccttcca gacgagagaa tcgagccgcc cccttggact ccccctcctg
     1501 gtagaccacg cgcgccgcct cgtcattgct caggccaggg gcgaacgtcg gatcgggatc
     1561 ctcgatctcg atccaaccca cctgatacgt ggtccctgga acctgagcgg cgtgaagatc
     1621 gacggtcacc ggttctgagg caccgggagg aatgacaccc agtatgtgca atgcacctcc
     1681 gtcggcgatt cgcttgtcct gctgtggccg gttgggcggg cgatagcggt agaagcccga
     1741 ggcataggcg aagtcgtcct ctgtcatgta cacatcgccg gtcgccgggt cgacggcggc
     1801 agcctcgtgg gcgaagcgcc cagcggacac gatcggatcg gcgatctcca gctcaccggg
     1861 ccccctcgag accggaacct cgaacaggta gccgtgtttc tgggtgtagg tgagatcgtc
     1921 cgggtcgttg ggcccgatca ggaagctgcg ctgctgatca atcccattcg gcgtctcctc
     1981 gcaggtcagc caggtccccc acgggctggc accaccggca cagttgaatg tggtgccgtt
     2041 gaggcttacc caactgctca cctcgtccgc ggtcggggat atctccagcg tcgtcgtgcc
     2101 gccaggagcg gcgggatcgt aggccttgct gacgtctccg aaggccccca cagggttggg
     2161 ctgagtctgc tcatgattgc gg
//
LOCUS       DASTXO010000413         4903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1130163, whole genome shotgun sequence.
ACCESSION   DASTXO010000413 DASTXO010000000
VERSION     DASTXO010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130163"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..907)
                     /locus_tag="VFU96_08665"
     CDS             complement(146..907)
                     /locus_tag="VFU96_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113376.1"
                     /translation="MFAVALVSCGDTPAAETTTSSSSSTTVVSTSTTTTMPPPGKPVA
                     LSDLVGRWESEAYAMTFDEGGSYELFELDESGTEVPTDVIGFVALEGDQLVFVTNANP
                     NPCAGQTGVYDGELVGEALQLALVEDPCAFREDAFASAFSRVSAPTTTGPEPVISDLS
                     GSWENERSVLRVNDAGDFVVLGPDADPDRPLTGGFVAREEGNFIFVSGVAGECPGQTG
                     VYGADVEEDTLKFIVVDDPCTSRVAWFEPTYTRAS"
     gene            complement(943..2436)
                     /locus_tag="VFU96_08670"
     CDS             complement(943..2436)
                     /locus_tag="VFU96_08670"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009612678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEU5113377.1"
                     /translation="MRQIQHWIDGQLTGGATERVGPVYNPATGEQTGEVVLGTAADVN
                     RAVETARTAFDTWRFSSLTQRQNIMFAFRELVATRKMEFAQLLTAEHGKTIDDALGEV
                     QRGLEVVEFACNIAHLLKGDFSEQVSTGVDTYTIRQPLGVVAGITPFNFPAMVPMWMY
                     PLAIACGNTFVLKPSEKDPSSSLLAAELLAEAGLPEGVFNVVHGDKEAVDALLTHPLV
                     SAISFVGSTPIARYIYETGTSHGKRVQALGGAKNHMIVLPDADMDLAADAAVSAGYGS
                     AGERCMAISAVVAVGDAGDSLIEGVRTRIEKLKVGPGHETGVEMGPLVTRQHLEKVRG
                     YVDAGEKAGARLVADGRDLVVDGHEKGFFLGPTLFDDVTTDMSIYTDEIFGPVLSTVR
                     MDHFEEAIRLINENPYGNGTAVFTNDGGAARKFQSEIEVGMVGINVPIPVPLSFYSFG
                     GWKHSIFGSHAIYGPEGVHFYSRQKVVISRWADPIHRGVDLGFPTHG"
     gene            2530..3405
                     /locus_tag="VFU96_08675"
     CDS             2530..3405
                     /locus_tag="VFU96_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEU5113378.1"
                     /translation="MVKFKRDATGPGQVIDRRGAGGGGYRPGMRTIGVGGGAIGVIIV
                     LLLTLLGGGGDLSGLTEALQPAGEVQPETGNTLDPSVDPEADLARYLEEVLQDTQAMW
                     VGVFEESQLDYRGTDLVVFNGFTQSECGGAQAQSGPHYCPIDELVYMDLDFLTELQRR
                     FGAAGDTAQAYIVAHEVGHHIQHQLGITEQVQSAGTNEASVALELQADCFAGVWLSTL
                     KSGESAALLESGDLEEALNAAAAVGDDAIQQQTSGQVNPETWTHGSSEQRYQWLNQGF
                     TSGSPGSCDTFAGTA"
     gene            3511..4230
                     /locus_tag="VFU96_08680"
     CDS             3511..4230
                     /locus_tag="VFU96_08680"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEU5113379.1"
                     /translation="MSLLTTAIPLIIYAVGFQTLVYWLVPIRFRPTWAPLATSSVGAA
                     LVVVAAVWFGPTTIGLAGPDGGLLASWGSATLVSMCTVGVVMMSRPQLRGHLADPRMG
                     AMSRRTAAAHILVRIPLITVVIEEAVFRGVLHAALIAVYPEPLALWGGAFLFGLWHIG
                     PGLDQAHAVDRRSLARVAHVAITVIATTAAGAFLVWLRIETGSIWVPIAVHAGVNMTL
                     ATFARLATASPASRPAALAVD"
     gene            complement(4258..>4903)
                     /locus_tag="VFU96_08685"
     CDS             complement(4258..>4903)
                     /locus_tag="VFU96_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017567840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5113380.1"
                     /translation="GLNVFAETCPQYLFFDLDNLGNGFEGAKYVCSPPMRDRAAGHQD
                     ALWQGLRTNDLQVVSTDHCPFCFNDHPELGRQKRLGEEDFSKIPNGMPGVEPRMEVLF
                     EGGVNQGRIGVNRFVEITATTPAKMFGMYPRKGTVAPGGDADIVVFDPNEKHTISVDN
                     QHMRVDYSAYEGMEVTGKVKCVLSRGRIIVENGEYLGNPGDGQYLKRGTSQYLI"
BASE COUNT          861 a         1601 c         1538 g          903 t
ORIGIN      
        1 tcgatggggt ccaactacaa catggttcgc cgtccagcgg tggtgttcgt cagcgatggc
       61 caagcgcgcc tcgtggtcag gcgagaaacc gatgacgatc tgttggcacg agacatgatc
      121 gccgggccca actagtcgtc acgcttcagc tcgctcgggt gtatgtgggc tcgaaccagg
      181 ccaccctcga ggtgcaggga tcatcgacga caatgaattt gagcgtgtcc tcctcgacat
      241 ctgcgccgta gacgccggtt tgaccgggac actccccggc gacaccggaa acgaaaatga
      301 agttgccctc ctccctcgcc acgaagcctc cggtgagcgg gcggtccgga tcggcatctg
      361 gcccgaggac gacgaagtcc cccgcgtcgt tgacccgaag aaccgatctc tcgttctccc
      421 aactcccgga caggtcggag atgacaggtt ccggcccggt ggtggtcggc gcggataccc
      481 gggagaaggc tgaggcgaaa gcatcttcac gaaaagcgca agggtcttcg accagtgcca
      541 gttgcaaggc ctctccgacg agctcgccgt catagacccc cgtttgaccc gcacaggggt
      601 tgggattggc attggtgacg aaaacgagct gatcgccctc gagggcaacg aacccgatca
      661 catcggtggg tacctcggtg ccgctctcat cgagctcgaa gagctcatac gaaccacctt
      721 catcgaacgt catcgcatag gcctcgctct cccacctgcc gacgagatcg gagagggcaa
      781 ccggcttgcc cggcggtggc atcgtggtcg tggtcgaggt gctgaccacg gtggtggaac
      841 tcgaagagga tgtggtggtc tcggccgccg gtgtatcccc acacgagacc agagcgaccg
      901 caaacaccac gagcaagggc cagacccgaa gtgggcggcg tctcacccgt gagtcgggaa
      961 cccgaggtcc accccgcggt ggatcgggtc ggcccagcgg gagatcacca ccttctgcct
     1021 gctgtagaaa tgcacgcctt cggggccgta gatggcatgg ctgccgaaga tcgaatgctt
     1081 ccacccgccg aagctgtaga aggagagcgg caccgggatc ggcacattga tcccaaccat
     1141 ccccacctcg atctcggact ggaacttgcg ggccgcgccg ccgtcgttgg tgaacacggc
     1201 ggtgccgttc ccgtatggat tctcgttgat gagcctgatc gcctcttcga aatggtccat
     1261 cctcaccgtg gacaggacgg ggccgaagat ctcatccgtg tagatcgaca tgtcggtcgt
     1321 cacgtcgtcg aagagcgtgg gtccgaggaa gaagcccttc tcgtgtccgt cgacgaccag
     1381 gtcgcggccg tcggcgacga gccgggcgcc tgccttctca ccggcatcga cgtagccacg
     1441 gaccttttcc aggtgttgcc tggtcacgag agggcccatc tcgaccccgg tctcgtgacc
     1501 tggccccacc ttcagcttct cgatcctcgt cctcacccct tctatgagcg agtcgccggc
     1561 gtcgcccacc gcgaccaccg ccgaaatcgc catgcaccgc tctccagccg agccgtaacc
     1621 ggccgagacc gcggcgtcgg ccgccaggtc catatctgca tcggggagga cgatcatgtg
     1681 gttcttggcg ccacccaacg cctgcacccg cttgccgtgg ctggtcccgg tctcgtagat
     1741 gtagcgagcg atcggggtag acccgacgaa ggagatggcg gagaccagcg gatgtgtgag
     1801 cagagcgtcc accgcctcct tgtcaccatg gacgacattg aatacgcctt ccgggagacc
     1861 ggcctcggcg agcagctctg cggccaggag cgacgacgaa gggtccttct cagagggctt
     1921 gagcacgaac gtgttgccgc aagcgatggc aagcggatac atccacatcg ggaccatcgc
     1981 ggggaagttg aacggggtga tcccggcgac gacgccaagc ggctggcgaa tcgtgtaggt
     2041 gtcgacgccc gtcgacacct gctccgagaa atctcccttt aggaggtggg cgatgttgca
     2101 ggcgaattcg acgacttcca atccccgttg cacctcgccg agggcatcgt cgatggtctt
     2161 cccatgttcc gcggtgagga gctgggcgaa ttccatcttc cgcgttgcga ccagttcgcg
     2221 gaaggcgaac atgatgttct gacgctgggt cagtgaggag aaccgccacg tgtcgaacgc
     2281 cgtccgggcc gtctccaccg cacgattcac gtcggcagcc gtgcccagca ccacctcgcc
     2341 ggtctgttcc cccgtggccg ggttgtacac gggcccgacc cgctctgtgg cccctccggt
     2401 gagctgcccg tcaatccagt gctggatctg cctcatagcg cgaatctaca cctggagggg
     2461 acgtacgacc gggtcgggga aatccgcgga aaatgcttag gccggcccgt tcctgggtac
     2521 atatgtcccg tggtcaaatt caagagggac gccaccggcc ccggccaggt gatcgaccgc
     2581 cgcggtgcag gaggcggggg ttaccgcccc ggaatgcgca cgattggggt gggcggtggt
     2641 gccattggcg tcatcatcgt cttgctactc accctcctcg gtggcggggg cgatctgagt
     2701 ggcctcaccg aagccctaca gccggccggc gaggtccagc cggaaacagg gaacacactc
     2761 gatccgtctg ttgatcccga agccgacctg gctcgctatc tcgaagaagt gctgcaggac
     2821 acccaggcga tgtgggtggg cgttttcgag gaatcccaac tcgattacag gggcacggac
     2881 ctggtggtgt tcaacggttt cacccagtcc gagtgtggtg gggcgcaggc gcagtctggc
     2941 cctcactact gcccgatcga cgagctggtc tacatggacc tggatttcct caccgagttg
     3001 cagcgccggt tcggcgcggc cggtgacacc gcccaggctt acatcgtcgc ccacgaggtc
     3061 ggccaccaca tccagcacca gttgggcata accgagcagg tgcaaagtgc gggcaccaat
     3121 gaggcgagtg tcgccttgga gcttcaggcc gactgttttg ccggtgtctg gctctccaca
     3181 ttgaagtcgg gggaatctgc cgccctcctc gaatctgggg acctcgaaga agccctgaac
     3241 gcggcggcgg cggtcggtga cgacgccatc cagcagcaga ccagcggtca ggtgaacccc
     3301 gagacgtgga cccacggcag ctcagagcag cggtatcagt ggctgaacca gggattcacc
     3361 tctggctccc ccggcagctg cgacacgttc gccggcaccg cctagtggcg gcgaaacccg
     3421 tatttgggtc tgttccttta ggtcctgtat accgataccg tggcatcgtg agtagctgac
     3481 cccgggtcgc cgactccatc tcgccaaacc atgtcattac tcaccacagc catcccgctc
     3541 atcatctacg cagtcgggtt ccagaccctg gtctactggc tcgtgccaat ccgcttccgt
     3601 cccacgtggg cgccgctcgc caccagctcg gtgggggcgg cgttggtagt ggtcgcggcg
     3661 gtgtggttcg gcccgacgac catcggcctc gccggccccg acggagggct tctcgcgtcc
     3721 tggggctcgg cgaccctggt ctcgatgtgc acggtcggag tggtgatgat gagccggccc
     3781 cagctgcggg gccatctcgc cgacccccgg atgggggcga tgtcgaggcg aacagcagcc
     3841 gcccacatcc tcgtccgcat cccgctgatc acagtcgtga tcgaggaggc cgtattccgc
     3901 ggtgtgctcc acgccgcctt gatcgctgta tatcccgagc cgctggctct atggggaggg
     3961 gccttcttgt tcgggctttg gcacatcggc cccgggctcg accaagctca tgccgtcgac
     4021 cgacgcagct tggcccgtgt cgcccacgtc gcgatcaccg tcatcgccac caccgccgcc
     4081 ggtgcattcc tggtctggct ccgcatcgag accgggtcga tctgggtgcc gattgcggtt
     4141 cacgccgggg tcaacatgac actcgcgaca ttcgcacggc tcgccaccgc gtcgccggcg
     4201 tcacgaccgg ctgccctcgc cgtcgactga tcgccgtact tttcggccgg caatcagtca
     4261 gatcagatac tgactggtgc cccgcttcag gtactgaccg tcgcccgggt tgcccagata
     4321 ttcaccgttc tcgacgatga tccgaccccg ggacagcacg catttgacct tcccggtcac
     4381 ctccatgccc tcgtaggcgg agtagtcgac ccgcatatgc tggttgtcca ccgagatggt
     4441 gtgcttctcg ttgggatcga agacaacgat gtcggcgtcc ccacccgggg ccaccgtccc
     4501 cttcctgggg tacatgccaa acatcttggc cggggtggtg gcggtgatct cgacgaaccg
     4561 gttgactccg atccgaccct ggttgacgcc accctcgaag agcacctcca tacggggctc
     4621 gacgccgggc atgccgttgg ggatctttga gaagtcctcc tcgccgagtc gcttctgccg
     4681 acccaattcc ggatgatcgt tgaagcagaa cgggcaatga tctgtagaga ccacttggag
     4741 gtcgttggtc cgcagccctt gccagagggc gtcctggtgg cccgccgccc gatctcgcat
     4801 cgggggtgag cagacgtact tcgctccctc gaatccgttg cccagattgt ctaggtcgaa
     4861 gaaaaggtac tggggacacg tctcggcgaa cacattgagc ccg
//
LOCUS       DASTXO010000414         2814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_540949, whole genome shotgun sequence.
ACCESSION   DASTXO010000414 DASTXO010000000
VERSION     DASTXO010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540949"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VFU96_08690"
     CDS             <1..195
                     /locus_tag="VFU96_08690"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5113381.1"
                     /translation="PEHGYVISGKMGIQLGFELFELEPGDSIAFDSTEPHRLFNMGEE
                     PVHAIWFVVGRGADDRAHGD"
     gene            203..1261
                     /locus_tag="VFU96_08695"
     CDS             203..1261
                     /locus_tag="VFU96_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006366457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5113382.1"
                     /translation="MPDDPITGSPVLVTGASGFIASHIVRELLEGGYRVRGTVRDPER
                     ARSEGHLTGLNGAAERLELTAADLLAPGAFDDAVRDCEFVIHTASPYTLDVNDPQRDL
                     VEPAVRGTISILESCRESNTTRRVVLTSSVAAITDQADGHLNTESDWNTRSSLTRNPY
                     YYSKTLAERAAWQFMEAAKSRFDLVAVNPFFVIGPSLGPTVNTSHASLIGLTNGNIPV
                     VLALTWPFVDVRDVARAHILAMENPAAAGRYLVAAETRTMRQVVDLLRANGWAARYKL
                     PSIGLDNAVGVALSRLVAGFQPSGTRDYMRSHLGGEMRFDNSKAKRELGIEFRDVDQT
                     ILDTMDDLERWGHLGKKR"
     gene            complement(1270..1884)
                     /locus_tag="VFU96_08700"
     CDS             complement(1270..1884)
                     /locus_tag="VFU96_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113383.1"
                     /translation="MSRRLIAVVLSLVLGVGCSGSSGVSQTIAPEVTVTTIALSTTAP
                     TVASSTTSTTTVVTTEAPAPTTTAQLSGPSGSGCTPGETIALPEGEWYGSVVSASASA
                     IEFDLACWFTGEAAVDAAAEDGAESPPPNDYYVRNDNALIRSLPVSPDTEVTWYPVGG
                     DPTSEEVATFQDWVEGAAARGYFLAVWLEVIDGEVLRIREQWVP"
     gene            complement(1881..2018)
                     /locus_tag="VFU96_08705"
     CDS             complement(1881..2018)
                     /locus_tag="VFU96_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113384.1"
                     /translation="MSEHPSAALDRRMLPRFEESDVGAFEDAIADDVLRWFPRSIRMI
                     P"
     gene            2152..2475
                     /locus_tag="VFU96_08710"
     CDS             2152..2475
                     /locus_tag="VFU96_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017985163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEU5113385.1"
                     /translation="MALPEVEERTSHGEPAWFIRGKRQFVTYADHHHDDRVAFWCAAP
                     DGGQRVLTGDDPDRYFVPPYVGGRGWVGCYLDVEVDWDALEEIVVDAYVTVAPARLAA
                     QVAQG"
     gene            complement(2494..2679)
                     /locus_tag="VFU96_08715"
     CDS             complement(2494..2679)
                     /locus_tag="VFU96_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113386.1"
                     /translation="MSSDIHQISTERERQSDLEFARKAVEYLGKKGLGPEEVIDYLVE
                     EFNLDRDTARTLAARAA"
BASE COUNT          514 a          905 c          880 g          515 t
ORIGIN      
        1 ccggagcacg gctacgtgat ctctgggaag atggggatcc agctcggttt cgagctattc
       61 gagctggagc ccggggactc catcgcgttc gactccacag agccccatcg tctcttcaac
      121 atgggagagg agccggtgca cgccatatgg ttcgtggtgg gccggggagc tgacgaccga
      181 gcccacggcg actgaccaac ccatgcccga cgatccgatc accggctcgc cggtcctcgt
      241 caccggggct tcgggattca tcgcgtctca catcgtcaga gagctgctcg agggcgggta
      301 ccgggtgcga ggcacggtgc gcgatccgga aagagctcga tctgagggcc acctgaccgg
      361 tctgaatgga gcagccgagc gactcgagct gaccgccgcc gatctcctcg ctcctggcgc
      421 attcgacgat gccgtccggg actgtgagtt cgtgatccac acggcgtcgc cctacacact
      481 cgacgtcaac gacccgcagc gggacctcgt cgaacctgca gtgcgcggaa cgatctcgat
      541 cctcgagtca tgccgggaaa gcaatacgac gcgaagagtg gttctgacct cgtcggtcgc
      601 cgccatcacc gaccaggctg acgggcacct caacacggag agtgactgga acacccgctc
      661 atcgctgacc cgaaatccct actactactc gaagacactg gccgagcggg ctgcctggca
      721 gttcatggaa gccgccaagt cacgattcga cttggtcgcc gtaaacccct tcttcgtcat
      781 cggcccatcc ctcggcccga cggtcaacac cagtcatgcc tcgttgatcg gcttgaccaa
      841 tgggaacatc ccggtggttc tggccctgac ttggccgttt gtggatgtcc gagacgtggc
      901 ccgcgcccac attctcgcca tggagaaccc ggctgccgcc gggcggtacc tcgtcgcggc
      961 cgagacgcga accatgcgcc aggtcgtcga cttgctgcgg gccaacgggt gggcggcacg
     1021 ctacaagctt ccctcgatcg gcttggacaa cgcagtggga gtcgctctca gtcgtcttgt
     1081 cgccggattc cagccgtcgg ggacgaggga ttacatgcga agtcatctcg gtggtgagat
     1141 gcggttcgac aactccaagg cgaaaagaga gctgggtatc gaattccggg acgtcgacca
     1201 gaccattctc gacacgatgg atgatctcga gagatggggg catctgggaa agaagaggta
     1261 ggcagggtct caggggaccc attgctcccg gatgcggagc acctccccgt cgatgacctc
     1321 cagccagaca gccaagaaat atcctctcgc cgctgccccc tcgacccaat cctggaaggt
     1381 ggcgacttcc tcagacgtcg gatcaccacc cacgggatac caggtgacct cggtgtcagg
     1441 cgaaaccgga agcgatctga tcagtgcgtt gtcgttgcgc acgtaatagt cgttcggtgg
     1501 cggtgattcg gctccatcct cggctgccgc atccaccgcc gcctcgcctg tgaaccaaca
     1561 cgccaagtcg aattcgatgg cgctcgccga cgcgctcacc acgctgccgt accactcccc
     1621 ttcggggagg gcgatggtct cccctggcgt gcacccggat cccgagggcc cgctgagctg
     1681 ggcggtcgtc gtgggtgccg gagcctcggt ggtgaccacg gtcgtcgtcg aagtcgtgga
     1741 cgaggcgact gttggcgccg tcgtcgagag ggcgatcgtc gtgaccgtca cttccggcgc
     1801 gatggtctgg gagaccccgc tcgagccgga gcagccgact cccaggacca gtgacaacac
     1861 gaccgcgatc agacgtcggc tcacgggatc atccgaatcg agcgaggaaa ccacctgagc
     1921 acgtcgtcgg ctatcgcatc ttcgaaggcg ccgacgtcgc tttcttcgaa tcgcggcaac
     1981 atcctgcgat ccaacgcggc gctcgggtgc tcgctcatgc cttcctcctt ctcgacagag
     2041 cgacatcgga aaggtagcga atcgccatac attcgtgcct gcaatcgcaa atacgattgg
     2101 gcgggaatgg gcccgacagc agatcaccta tcccgacttc gaggcatttg catggcgttg
     2161 ccggaagtgg aggagcggac gagtcatggc gaacccgcct ggttcataag ggggaaacgg
     2221 cagttcgtga cctacgcaga tcaccatcac gacgaccgcg tcgccttctg gtgtgcggca
     2281 cccgacgggg ggcaacgggt tctcaccggc gacgatcccg atcgctactt cgtcccgccg
     2341 tacgtcggtg ggcggggctg ggtggggtgc tatctggacg tcgaagtcga ttgggacgcg
     2401 ttggaggaaa tcgtcgtgga cgcctatgtc accgtggcgc cggctcgact ggcggcccag
     2461 gtcgcccaag ggtgaaccac cgttggattg gagtcaggcg gccctcgccg cgagggttct
     2521 ggcggtatcg cggtcgaggt tgaactcttc gaccagatag tcgatgacct cctcggggcc
     2581 gaggcccttc ttcccgaggt actcgaccgc cttcctggcg aattcgagat cggactgacg
     2641 ttctctctcg gtcgagatct gatggatgtc gctgctcatc tcgcactccc gtatcgcaga
     2701 atcacttccc acgccccgcc gtcgacaaac ttcagactcg ccggcggtgg atatggttcc
     2761 cgattcttca acggtctcca aggaccctcg gtccgggcgg tcagccggcc accc
//
LOCUS       DASTXO010000415         3695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1424715, whole genome shotgun sequence.
ACCESSION   DASTXO010000415 DASTXO010000000
VERSION     DASTXO010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424715"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /gene="ligD"
                     /locus_tag="VFU96_08720"
     CDS             <1..1126
                     /gene="ligD"
                     /locus_tag="VFU96_08720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEU5113387.1"
                     /translation="VGRTDDRLTFQLKGDRLAGIWHLVHTGLKDGKDQWLAMMSKDLR
                     PASEGRPPPRPMLATLTAEAFDDPEWGYEPKWDGIRAIAMCDEDTRLITRNDKDVTVA
                     YPELHRLHEQTVALEAMLDGEIVAFENGVPSFQRLQQRMHLRDEQKVKEMARQIPVVY
                     IVFDLLYLDSRDLTGRPLEERRRILEDTIVPSDHVQVSPMTEGEGAALFRAAAEQGLE
                     GIMAKRLSSRYQPGERSKDWLKVKVTFDADVVIVGWTEGEGSRAGSLGSLMMAVYDIG
                     ELRYVGNVGTGFGRGLLEEAFDRLNALPESETPFPPEVLRSRPELRRAHWVQPSLVAM
                     AEHRGLTSAGRLRAPSFKGFREDKAPEDCTFDQFAEVPAP"
     gene            1165..2277
                     /locus_tag="VFU96_08725"
     CDS             1165..2277
                     /locus_tag="VFU96_08725"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEU5113388.1"
                     /translation="MTTIRGTEEQGATFVELFFDLVFVFAVTQVTATLAHDLTAAGLL
                     RALIVFWLVWWAWTQYTWSLNEADTEHVSIRLITLVATALAFLMAVTVPLITSPFGWL
                     FPISYLVLRMVGISLQWRLAAGDSVWARSVRTWTVLSSLGLLAVIAAVFLPADLRFVA
                     LGVAALLDVLAAVRAGRGEWRLFPGHFSERHGLFVIIVLGESLIAAGVTVTDQSLSVR
                     LLLVAVTAVAGTCALWWTYFGWAKDALEERMRSQSPQSLGRFARDVYSFGHFPLIFGV
                     IGFAIAIEEAIAHPKEPLPLPGALALCVGVGLIVGSTALAVKLAGVHVPIVRWWVIVV
                     LLALIPVLGAVSAWVALGLVSLLVAGLALAEEASRR"
     gene            complement(2278..3438)
                     /locus_tag="VFU96_08730"
     CDS             complement(2278..3438)
                     /locus_tag="VFU96_08730"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5113389.1"
                     /translation="MTERIRQITAANETLLSFLPLWEQHRHRPGVSNFAFGNPQELVV
                     DGFASAIASATTPLDKDHYAYKFSEVESQRTVANHLAEWRGQQFDPADIALTPGAFGA
                     IAIALGALVDFGDEVVYCDPTWFFYRSMILSAGATPVSVPVDSTSYDLDLDRLSNAIT
                     EKTRLVIVNTPHNPTGRIYPPATLEDLARVLTDASERFGAPIYLLSDEPYSKLVFSES
                     KFTSPAEYYSNTLIAYSYGKILLTPGQRIGWLALSPLMPDPEPVRSAIFMAQIAGGWL
                     FPSAIMQYSLAELDRLSIDLVELERKRDVFVEALARSGYELQPPEGTFYLWVRSPDPD
                     DVAFVERLADDGVLVLPGTTCASPGYFRISLTATAEMIEQSLPTFQTAVSKT"
BASE COUNT          619 a         1108 c         1220 g          748 t
ORIGIN      
        1 ggtcgggcga accgatgacc gtctgacctt ccaactgaaa ggcgaccgtc tcgccggcat
       61 ctggcacctg gtacacaccg gtctcaagga tggaaaagat cagtggctgg cgatgatgtc
      121 gaaggacctg cgcccggcga gtgaggggcg accgccgccc cgaccgatgc tggcgacact
      181 caccgccgag gccttcgacg acccggagtg gggctacgag ccgaaatggg acggaatccg
      241 ggccattgcc atgtgtgacg aggacacccg gctgataacc cgcaacgaca aggacgtcac
      301 cgttgcctat ccggagctgc accgcctgca tgagcaaacg gtcgcgctgg aggcgatgct
      361 cgacggagag atcgtcgcct tcgagaacgg ggttccttct ttccagcgtc tccagcagcg
      421 gatgcatctc agggacgagc agaaggtgaa ggagatggct cgccagatcc cggtggtcta
      481 catcgtcttc gacctgctct atctcgactc tcgggacttg accggtcgtc cgttggagga
      541 gcggcgcagg atcctggagg acacgatcgt cccttcggac cacgtccagg tctcgccgat
      601 gaccgaagga gagggagcgg cgctcttccg ggccgccgcg gaacaaggtc tggaagggat
      661 catggccaaa cgcctctcgt cccgatacca accgggtgag cggtccaagg attggctcaa
      721 ggtgaaggtg accttcgatg ccgatgtcgt gatcgtggga tggaccgagg gtgagggaag
      781 ccgggcggga agcctcggct cgctgatgat ggcggtatac gacatcggcg aactgcgcta
      841 cgtggggaat gtcggtaccg gctttggccg cgggttgctg gaggaggctt tcgatcgact
      901 caatgctctg cccgagtcgg agactccgtt tccgcctgaa gtgctgcgtt cccgtcccga
      961 gctgcgccgg gcccactggg tgcagccttc cttggtggcg atggccgagc atcgcgggct
     1021 cacctcggcc ggccgtctcc gggcaccttc gttcaagggc tttcgggagg acaaggcgcc
     1081 ggaggattgc accttcgatc agttcgcgga agtacccgcc ccatgagctg tcgtgcctcg
     1141 gtacggccgt aggatccacc ggccttgaca acgattcgcg ggaccgaaga gcaaggggcg
     1201 acgttcgtcg agctcttctt cgatctggtc ttcgtattcg cggtgaccca ggtgacggca
     1261 actttggccc acgatctcac cgcggccggt ctccttcggg cgttgatcgt cttctggctg
     1321 gtctggtggg cgtggactca gtacacgtgg tcgctcaacg aagccgacac cgagcatgtt
     1381 tcgatccggc tcatcaccct cgtcgccacc gccctggcct ttttgatggc ggtcaccgtc
     1441 cctctcatca ccagcccgtt tggctggctg tttcccatct cctacctcgt cctccggatg
     1501 gttggcatct cgctgcagtg gaggctcgcc gccggcgatt cggtctgggc ccgatcggtg
     1561 cggacatgga cggtcctgtc cagtcttggc ctgctggcgg tcatcgctgc cgttttcctt
     1621 cccgcggatc tccggttcgt agccctgggc gtggccgccc tactcgacgt gctcgcggcg
     1681 gtccgcgccg ggcgaggcga atggaggctg ttccccggtc atttctcgga gcggcacggt
     1741 cttttcgtca tcatcgtcct cggtgaatct ctgatcgcgg cgggtgtgac ggtcaccgat
     1801 caatcgctca gtgtcaggct gctgctggtg gcggtcaccg ccgttgccgg tacctgtgcc
     1861 ctctggtgga cctacttcgg ctgggccaag gacgccctcg aggagagaat gaggtcacaa
     1921 tctccgcaga gtctcgggcg attcgcccgg gacgtgtact cattcggtca tttcccgctg
     1981 atcttcggtg tcatcggttt tgcgatcgcc atcgaagagg ccatcgctca tcccaaagag
     2041 cctctcccgc ttccaggggc cctagcgcta tgtgtcggcg tcggcctgat cgtggggagc
     2101 acagccctgg ctgtgaagct ggccggggtt cacgtgccaa tcgttcgctg gtgggtgatc
     2161 gttgtgctct tggccctcat cccagtattg ggagcggttt ctgcctgggt ggctttgggc
     2221 ttggtatcgc tcctggtggc cggtttggcg ctggccgagg aggcgtcccg ccgatgatca
     2281 cgtcttggag actgccgtct ggaacgtggg aaggctctgc tcgatcatct cggcggtggc
     2341 ggtgagcgat atacggaaat accccggcga ggcacaggtc gtgcccggca acaccagaac
     2401 tccgtcatcg gcgagtctct ccacgaaggc gacatcgtcg ggatccgggg agcgaaccca
     2461 gagatagaag gtcccctcag gcggttgcag ctcataaccg gagcgcgcaa gcgcttcaac
     2521 gaacacatct cgcttgcgct cgagctcgac gagatcgatg gagagccggt ccaactcggc
     2581 gagtgagtac tgcatgatgg cgctggggaa caaccatccg cctgcgatct gggccatgaa
     2641 gatcgctgaa cgcaccggct cggggtcggg catgagtggc gacagtgcga gccagccgat
     2701 tctctggcca ggtgtgagca ggatctttcc gtagctgtag gcgatgaggg tgtttgagta
     2761 gtactcggcc gggctggtga atttcgactc gctgaaaacg agcttggaat agggctcgtc
     2821 ggatagcagg tagatcggcg ccccgaatcg ttccgaagcg tcggtcaaga ccctggccag
     2881 gtcttcgaga gtcgctgggg ggtagatgcg acctgttgga ttgtgcggcg tgttgacgat
     2941 caccaagcgt gtcttctcgg tgatcgcatt cgagagccgg tcgaggtcga ggtcgtagct
     3001 ggtgctgtcc acgggcacgc tgaccggtgt tgccccggcg gagaggatca tcgacctgta
     3061 gaaaaaccac gtcgggtcgc agtagacgac ctcgtctcca aaatccacca aggcaccgag
     3121 agcgatcgcg atcgctccga aggctccagg tgtcagcgcg atgtcggcag ggtcgaattg
     3181 ctgacccctc cactccgcca gatggttggc cacggtccgc tgtgactcca cctcgctgaa
     3241 cttgtaggcg tagtgatcct tgtccaaggg ggtcgtagcc gaggcgattg cggaggcaaa
     3301 cccatcgacg accaactctt gagggttccc gaaggcgaag ttggagacgc cgggccgatg
     3361 ccggtgctgt tcccacaggg ggaggaacga gagcagtgtc tcgttggctg ccgtgatttg
     3421 ccggatgcgc tcggtcacca tgcctgcagc cgacgatagc tgcagatgag gagcggccaa
     3481 taggggttgt ccccctgccg aaacggccct cggttcacgc cggcgtccag tcagccgacc
     3541 acgtcctcga gaaaaccggc gactctcgcg accatctccg ctccgtgact cagcgatggg
     3601 tcgttgtgat cggcgccctc gatgacgtgg atgcctttcc cgtccgttga caccatttcg
     3661 tacagctgtc tgctctgctc cggggggacg atggt
//
LOCUS       DASTXO010000416         2463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1351299, whole genome shotgun sequence.
ACCESSION   DASTXO010000416 DASTXO010000000
VERSION     DASTXO010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351299"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="VFU96_08735"
     CDS             <1..686
                     /locus_tag="VFU96_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEU5113390.1"
                     /translation="HVTKDGGLAGPKMLEHMVDVVLYLEGDPDRGFRVLRSTKNRFGA
                     THVSGMFDMRSEGLTEVEDPSKMFLDGWEPEVPGTVIYPAVEGRRSILVEIQALVSPT
                     NQAQPRRSIRGLDPTRVNQVLAVLDRHSSLILHDKEVYVNVVGGWRIDEPGCDLPVAL
                     AVASSYSDRPLGRLAAWGEIGLAGEVRSIPMEIRRIEELDRMGLEHRVAPLGGKPLRL
                     SEALDDAGL"
     gene            733..1794
                     /gene="disA"
                     /locus_tag="VFU96_08740"
     CDS             733..1794
                     /gene="disA"
                     /locus_tag="VFU96_08740"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:NF010009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HEU5113391.1"
                     /translation="MGGPSITETLERLAPGTPMRRALERIIQQGKGGLVVLGDGAQVN
                     EASSGGFSLKGAIFSPARLAELSKMDGGIILDDSWSNILEAGVHFIPDGSIPTDETGA
                     RHRTAERMAVQTGKPVVAVSEGRKVATLFYGGQKIELASPTEVAAHVNQELQSLDRLR
                     GRLDEAETKLTRLEVAGLATYRSAVTVLQRAELVERLGRVVEARTLTLGDEGRIVYLQ
                     LSDLLAGVDFTKRALLRDYIKPLRSSTIRQAMEEMSAMSASDLEDPSRVGKELGFPDL
                     DDPADPRGYRLLSQVGRLPDSVRDDIIRHFGSASSLLEARVDELLDVEGVGETRAGQL
                     RAYFDRLRDNADEWEPVLD"
     gene            1826..2338
                     /locus_tag="VFU96_08745"
     CDS             1826..2338
                     /locus_tag="VFU96_08745"
                     /inference="COORDINATES: protein motif:HMM:NF044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEU5113392.1"
                     /translation="MAATATKYSVGDKVVHPQHGAATIQKKVRQEFGGKKQDYFVLDI
                     ATEQLTVMVPLDKVDDLIRPVISKTKSRDVLKSLKGEPEEAGSNWSRWYKVLNEKMTS
                     GDIFQVAEVVRDLSYAQQTKGISPALKRMLSKARLTLSSELAFSLEIDEEEAIKRLDR
                     ALPKVESDEE"
BASE COUNT          467 a          746 c          830 g          420 t
ORIGIN      
        1 gccatgtcac caaggatggc gggctggccg gccccaagat gctcgagcac atggtggatg
       61 tggtcctgta tctggaaggc gaccccgatc gtgggttcag ggtcctccgc agcaccaaga
      121 atcgctttgg cgcgacccac gtctcaggga tgttcgacat gaggagcgag ggcctcaccg
      181 aggtcgaaga cccatcgaag atgtttctcg acggttggga gcccgaagtc cccgggacgg
      241 tcatctatcc ggccgtggag ggccggcggt cgatcctggt cgagatccag gctttggtct
      301 cgccgacgaa tcaagcacag cctcggcgat cgatccgggg gctggacccg acgagagtca
      361 atcaggtgct cgctgtgctc gaccgccaca gctcactgat cctccacgac aaggaggtct
      421 acgtcaatgt cgtgggagga tggcgcatcg acgagcccgg ctgcgatctc cccgttgcac
      481 tcgcggtcgc atcgtcgtat tccgaccggc cactgggccg gttggcggct tggggagaga
      541 tcggcctagc cggggaggtt cgctcgatac caatggagat caggcgaatc gaggagctcg
      601 accggatggg gctggagcat cgggtcgcac cactcggtgg caagccactc cgtctcagtg
      661 aggctctcga tgacgccggc ctctagaact tcccctgggt gagcgtcaac tgaatcgggt
      721 actctgaggg ccgtgggtgg accgtcgata accgagacgc tcgaacgttt ggctccgggg
      781 actccaatgc ggcgagccct cgagcgcatc atccagcagg gcaaaggcgg cttggtcgtg
      841 ctgggcgacg gcgctcaggt gaatgaggcc agctcgggtg gcttcagttt gaagggggcc
      901 atattcagcc cggcccgtct cgccgagctc tccaagatgg atggtgggat catcctcgac
      961 gactcatggt cgaacatcct cgaagccggc gtgcacttca tccccgatgg ttcgatcccg
     1021 accgacgaga cgggcgcacg ccatcggaca gccgagcgca tggccgtgca gaccggcaag
     1081 ccggtggtcg cggtgagcga gggtcgcaag gtggcgaccc ttttctacgg cggccagaag
     1141 atcgagctgg catcaccgac ggaggtggcg gcccatgtca accaggagct gcagagcctc
     1201 gatcggttac gtggccggct cgacgaggcg gagaccaagc tgacccgtct cgaagtggcc
     1261 ggtttggcca cctaccggtc ggcggtcacc gttctgcagc gcgccgagtt ggtggaacgg
     1321 cttgggcgag tggtggaggc gcgcacgctg accctgggag atgagggccg catcgtgtac
     1381 ctgcagctca gcgatctgct cgccggcgtc gatttcacga agagggccct gctcagggac
     1441 tacatcaagc cgctccgcag cagcaccatc cgccaggcga tggaggaaat gagtgcgatg
     1501 tccgcctccg atctggaaga tccgagccgg gtaggcaagg aactgggatt tcccgatctc
     1561 gatgacccag ccgatccacg cggctatcgc cttctctccc aggtcggacg tctccccgat
     1621 tcggtgcgtg acgacatcat ccggcatttc ggctcggcct cgagcctcct cgaagcgcgg
     1681 gtcgacgaat tgctcgacgt ggaaggcgtg ggcgagacca gggccgggca actgcgcgcc
     1741 tacttcgacc ggcttcggga caacgcggac gaatgggagc cggtgctgga ttgagcgaag
     1801 gctcccctac actccgctcc cgctaatggc tgcgacagca acgaaatact cggtagggga
     1861 caaagtggtc cacccccagc acggggcggc caccatccag aagaaggtca ggcaggagtt
     1921 cggggggaag aaacaggact atttcgtcct cgacatcgcc accgagcagc tgaccgtgat
     1981 ggttcccttg gacaaggtcg atgacctgat ccgccccgtg atctccaaga ccaagtcacg
     2041 tgacgtgctg aagtcgctga agggggagcc cgaggaggca gggtccaact ggagccggtg
     2101 gtacaaagtg ctcaacgaga agatgacctc gggcgacatc tttcaggtgg ccgaagtcgt
     2161 gcgtgacctc tcctatgccc agcagacaaa ggggatcagc ccggccctga aacggatgtt
     2221 gtccaaggcg cgtctcaccc tcagctccga gctcgccttc agcctcgaga tcgatgagga
     2281 ggaggcgatc aagaggctgg atcgggcgct gcccaaggtc gaatcggacg aggaatgagc
     2341 tcggtgccgc atgccggcgc ccgtgtcgga tggggattcg acgcacaccc tctcgacggc
     2401 gagccacccc tgatcgttgg tggggtgagc gtctcgagcg ccgttggcgc aagtgccacc
     2461 tcc
//
LOCUS       DASTXO010000417         3494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_872340, whole genome shotgun sequence.
ACCESSION   DASTXO010000417 DASTXO010000000
VERSION     DASTXO010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872340"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VFU96_08750"
     CDS             complement(<1..878)
                     /locus_tag="VFU96_08750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5113393.1"
                     /translation="MSRRAEVVVVGAGIAGVAAAYELTVKHGIGNVVIVDPRPPMSLT
                     SDKSTECYRNWWPNLPMVGLMTRSIDLLERMAAESGNVFALNRRGYLFVTADEENFHR
                     MVEDAHHTSTLGAGPIRLHPGPIPYRNSPSDGYVGAPEGADILVGTEEVLDHFPYLTP
                     EAVGAIHVRRAGWFSAQQLGAWMLDQSKDRGLEVVVDEVAGIGESAGSIRSVSLAGGG
                     EIATETVVDAAGPMAGNVAAMAGVDLPLFSELHLKAAYRDHLGVVPRDAPMVIWSDKQ
                     RLGWSDEERDALGGEGR"
     gene            complement(880..2826)
                     /gene="tkt"
                     /locus_tag="VFU96_08755"
     CDS             complement(880..2826)
                     /gene="tkt"
                     /locus_tag="VFU96_08755"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU5113394.1"
                     /translation="MANNGIEKLAVDTIKALAMDAVQKANSGHPGMPMGMAEIAVTLW
                     GRFLKVDPAVPDWPDRDRFVLSNGHGSMLLYSMLHLSGFPLTMDDIKNFRQWGSHTAG
                     HPEIDQKLGIEMTTGPLGQGFATGVGMAIAETHLRARLGEDLVNHRTFGFVSDGDLME
                     GVSAEAASLAGRFGLGKLIYLYDDNDISIDGSTDITFAEDVPKRFQAQGWHTQEIDGH
                     DPEAIDEALGAAINLEDRPSLIICHTHIAHGAPNAQDTAASHGSPLGDEEVALAKEAM
                     GFPLQPTFHVAPEVYEFFETAMAWGKEERANWANRLETADEDKRALWEQLHSPAPVEL
                     KGPGFGAGEKMATRSASGKLFTEIAEQVPGFIGGSADLVASTKTVIDPERRYSPSDRA
                     ARDIPFGVREHAMGCITNGMAVHGGLKPYAATFFVFSDYMRPAVRLSALMSAPSVWVW
                     THDSVFLGEDGPTHQPIEHLPSLRAMPTLWVIRPADANETLAAWEIALDRHDGPVGLL
                     LTRQDVPVLDTDPAGAKRGGYVLRDGDAVTLIATGSEVHVAVDAAGILEEQGISARVV
                     SLPCWELFFEQDEEYRSGVLGSSPRVAIEAASPFGWERIVGRDGLILGIDHFGESAPD
                     TVIAEKLGFTGAAVAARVRDHLSA"
     gene            complement(2863..>3494)
                     /locus_tag="VFU96_08760"
     CDS             complement(2863..>3494)
                     /locus_tag="VFU96_08760"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU5113395.1"
                     /translation="VVTFNIEGVSSLDLATEGYEKRFAGRHGRRLFRADPPAVWGRHI
                     PEAMEAGNPGRLPVDLRSRTPFQARVLRIAASIPRGEVRPYGWLAREAEKPGASRAVG
                     STMARNPIPLIIPCHRVVRSDGHLGEYSLGGPHNKWELLTHEGVEPARLVELAEHHVR
                     VQGDPTTGVYCHPTCHVIGASGQWNIVDFTSTTAARSAGFQPCEVCRPG"
BASE COUNT          583 a         1132 c         1179 g          600 t
ORIGIN      
        1 cggccctcgc caccgagcgc atcccgttct tcgtcgctcc agcccagcct ttgcttgtca
       61 ctccagatca ccatcggggc gtcgcggggg accacgccga ggtggtcccg gtaggcagct
      121 ttgaggtgga gctcggagaa cagtggtagg tcgactccgg ccatggccgc cacgttgccg
      181 gccatcggcc cggcggcgtc gaccaccgtt tctgtggcga tctcgcctcc gcccgccagc
      241 gacaccgatc ggatcgatcc cgccgattcc ccgatgccgg ctacctcgtc gaccaccacc
      301 tccagaccgc ggtctttgct ctggtcgagc atccaggccc cgagctgctg ggcgctgaac
      361 caaccagccc tcctgacatg aatcgcaccg acggcctccg gggtcaggta gggaaagtga
      421 tcgagcacct cctcggtccc gacgaggatg tcggcacctt cgggtgcccc gacgtaaccg
      481 tccgacgggg agttgcggta gggaatgggg ccgggatgga ggcggatcgg gccggcgccg
      541 agggtcgagg tgtggtgggc gtcctcgacc attcggtgaa agttctcctc gtcggcggtc
      601 acgaagagat agccgcggcg gttcagggcg aagacatttc ccgactcggc agccatcctc
      661 tccagcagat cgatcgacct ggtcatcaag ccgaccatcg ggaggttggg ccaccagttg
      721 cggtagcact cggtggactt gtccgaggtg agtgacatcg gaggtcgggg atcgacgatg
      781 acaacgttgc cgattccgtg cttcacggtg agttcatagg cagcggccac tccggcgatg
      841 cctgccccca cgacgacgac ctcggctctg cgactcacct caggcggaga gatggtcccg
      901 cacccgagcg gccactgccg cgccggtgaa gccgagcttc tcggcgatga cggtgtcggg
      961 agctgactct ccgaaatggt cgatgccgag gatcagcccg tcccggccaa cgatgcgctc
     1021 ccaaccgaac ggcgaggcgg cctcgattgc cacccgcggc gatgacccga gaacgccgga
     1081 tcggtattcc tcgtcttgct cgaagaacag ctcccaacag ggaagactca ccacccgggc
     1141 agaaatgccc tgctcttcga ggatgccggc tgcgtcgaca gcgacatgga cttcggaacc
     1201 ggtggcgatg agagtcactg cgtcgccatc tcggagcaca tagccacccc tcttcgcgcc
     1261 ggcagggtcg gtgtcgagca ccgggacgtc ctgacgggtg aggagaagcc ccaccgggcc
     1321 gtcgtgccga tcgagggcaa tctcccaggc cgccagggtc tcgttggcat cagcgggccg
     1381 gatgacccag agggtgggca tggcacgcag cgaggggagg tgctcgatcg gctgatgggt
     1441 cgggccgtcc tcgccgagga agacggagtc atgggtccac acccacaccg atggtgccga
     1501 catcagagcc gagagacgca ccgcgggccg catgtagtcg ctgaagacga agaaagtcgc
     1561 ggcatagggc ttgagcccgc cgtggaccgc cattccgttt gtgatgcagc ccatggcgtg
     1621 ttccctgacg ccgaatggga tgtcgcgcgc cgcccggtcg ctcggcgagt agcggcgctc
     1681 ggggtcgatc acggtcttgg tcgaggcgac gagatcggcc gatccaccga tgaacccggg
     1741 gacctgctcg gcgatctccg tgaagagctt gcccgaagcc gaccgcgtgg ccatcttctc
     1801 accggcgccg aacccggggc ccttcaactc gacgggggcc ggcgagtgca actgctccca
     1861 gagcgcccgc ttgtcttcgt cggcggtctc cagacgattg gcccagttgg cgcgctcctc
     1921 cttcccccag gccatggccg tctcgaagaa ctcgtagacc tcgggggcga catggaaggt
     1981 gggctgcagg gggaatccca tggcctcctt tgccagggcg acctcctcgt ctcccaaagg
     2041 tgagccgtgg gaggcagcgg tgtcctgggc gttgggtgcc ccatgtgcga tatgggtgtg
     2101 gcaaatgatg agcgacgggc ggtcctcgag gttgatggcg gccccgaggg cttcgtcgat
     2161 ggcttcggga tcgtgcccat cgatctcctg ggtgtgccag ccctgagcct ggaacctctt
     2221 cggcacatcc tcggcgaagg tgatgtcggt ggacccgtcg atcgagatgt cgttgtcgtc
     2281 gtagagatag atgagcttgc ccaggccgaa tcgaccggcc aaggaagccg cctcggcgct
     2341 gactccctcc atgagatcgc catcgctgac gaagccgaag gttctgtgat tcaccaggtc
     2401 ctcgccgagg cgggcccgaa ggtgtgtctc ggcgatggcc atgcccaccc cggtggcgaa
     2461 cccctggccg agtggcccgg tcgtcatctc gattcccagc ttctggtcga tctccgggtg
     2521 ccctgcggtg tgactgcccc attgccggaa gttcttgatg tcgtccatgg tgagcgggaa
     2581 gccgctgagg tggagcatgg agtacaggag catcgagccg tgcccgttgg agaggacgaa
     2641 tctgtcccga tcgggccaat cggggaccgc cgggtccacc ttgaggaaac ggccccacag
     2701 ggtcactgcg atctcggcca tccccatggg catcccggga tgcccgctgt tggccttctg
     2761 caccgcgtcc atggcgagcg ccttgatggt gtcgaccgcc agcttctcga tcccgttatt
     2821 ggccataggc cacgggaggc taccggcgtc ggtaggaggc ggctaaccgg gccggcagac
     2881 ctcacagggc tggaatccgg ccgatctggc agctgtggtc gacgtgaagt cgacgatgtt
     2941 ccattgcccc gatgccccga tgacatgaca ggtcggatgg caatagaccc ctgtggtggg
     3001 gtcaccctgg acccggacgt ggtgctcggc gagctcgacg agacgggcgg gctccacccc
     3061 ctcatgggtg agcaattccc atttgttgtg aggccctccc agggagtact caccgagatg
     3121 gccatcagat cgcacgaccc ggtggcaagg gatgatgaga gggatggggt tgcgagccat
     3181 ggtcgagccg accgctctgg atgcgcctgg cttctccgcc tcccgagcca gccagccgta
     3241 aggccggacc tcgccccgcg ggatcgaggc agcgatccgg agcacacgtg cctggaacgg
     3301 tgttcgcgac ctgaggtcga ccggaagccg gcccgggttc ccagcttcca tcgcctcggg
     3361 gatgtgccgg ccccagacgg ccgggggatc ggcccggaac agacggcgac catgtctgcc
     3421 ggcgaaccgc ttctcgtagc cctctgtggc caggtcgaga gacgagaccc cctctatgtt
     3481 gaaggtcact acca
//
LOCUS       DASTXO010000418         2084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1498515, whole genome shotgun sequence.
ACCESSION   DASTXO010000418 DASTXO010000000
VERSION     DASTXO010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1498515"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VFU96_08765"
     CDS             complement(<1..538)
                     /locus_tag="VFU96_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit D"
                     /protein_id="HEU5113396.1"
                     /translation="MTPEDVYADIRETTEGAVYNVSGQDWDDIVGAAVAGQERVVVNM
                     GPQHPSTHGVLRLILELDGETVTEARAGIGYLHTGIEKNMEYRTWTQGVTFCTRMDYL
                     SPFFNETAYVLGIERLLGITDQIPERATVIRVMMMEINRIASHLVAIATGGMEIGALT
                     VMTIGFRERERSLDIFELV"
     gene            complement(598..1443)
                     /locus_tag="VFU96_08770"
     CDS             complement(598..1443)
                     /locus_tag="VFU96_08770"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEU5113397.1"
                     /translation="MSDDRTPAHDEATAPVGGEAEKTSPPDAPATVDRPSAEATTPGP
                     GDLPEEVPDEGAAGLPPPHPTKRGMFGAAGTGDTSGFGRLVARDTNPIRVESTPRPWG
                     PEFDDVGDSLTGVLEQAGLPFDAAVEKVVVDRGEITFFVRREHLVEVARALRDDPALR
                     YEMCMGVNGVHYPGEAGRELHAVYPFLSVTHNRRVRVEVTCPDDDPHVPSIVELYPSN
                     NWHERETYDFFGIIFDGHPALTRIEMPDDWPGHPQRKDYPLGGIPVEYKGASIPPPDE
                     RRAYT"
     gene            complement(1440..1994)
                     /locus_tag="VFU96_08775"
     CDS             complement(1440..1994)
                     /locus_tag="VFU96_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEU5113398.1"
                     /translation="MGIEEKLPAGVLLTGVERLVGFMRKSSVWPATFGLACCAIEMMA
                     FGGPRHDSARFGMEVFRASPRQADLMIVAGRVSQKMAPVLRQIYDQMPEPKWVIAMGV
                     CASSGGMFNNYAIVQGVDHIVPVDIYLPGCPPRPEMLMDAILKLHDKILHTPLGPHRK
                     RAEAEAEEHALEAAPTHEMRGLLR"
     gene            complement(2009..>2084)
                     /locus_tag="VFU96_08780"
     CDS             complement(2009..>2084)
                     /locus_tag="VFU96_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017948722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEU5113399.1"
                     /translation="MVLFILTVFVAYAYVWRRGGLDWD"
BASE COUNT          339 a          698 c          679 g          368 t
ORIGIN      
        1 tgacgagctc gaagatgtcc agagaccgct cccgttcacg gaagccgatc gtcatcaccg
       61 tgagtgcacc gatctccatg ccaccggtcg cgatggccac caggtgcgag gcgatgcggt
      121 tgatctccat catcatcacc cggatgacgg tggcccgttc cgggatctgg tcggtgatgc
      181 ccagcagccg ctcgatgccc agcacgtagg cggtttcgtt gaagaacggc gacagatagt
      241 ccatccgggt gcagaacgtc acgccctgcg tccacgtccg gtactccatg ttcttctcga
      301 tgccggtgtg tagatatccg atgccggcac gggcctcggt gacggtctcg ccgtcgagtt
      361 cgaggatgag ccggagaaca ccgtgcgtcg acgggtgctg cgggcccatg ttgaccacga
      421 cccgctcctg gcctgccacg gcggcgccga cgatgtcgtc ccagtcctgg ccgctgacgt
      481 tgtagacggc gccttcggtc gtctcccgga tgtctgcgta gacatcttcg ggtgtcacct
      541 cgtccgcggg agccacgaag gtgacgtcat ccacgggcat ctggtcgcga tcggtgctca
      601 tgtgtatgcc ctccgctcgt ccggcggggg aatcgacgcg cccttgtact ccacggggat
      661 tcctccgagc ggatagtcct tgcgttgcgg atggcccggc cagtcgtcgg gcatctcgat
      721 cctggtcagt gctggatggc cgtcgaagat gatcccgaag aagtcgtatg tctcccgctc
      781 gtgccagttg ttcgacgggt agagctccac gatggacggc acgtgcgggt cgtcgtcggg
      841 gcaggtgacc tccactcgta cccggcgatt gtgggtgacc gacaggaacg ggtacacggc
      901 gtgcagttcg cgccctgcct caccgggata gtgcacgccg ttgacgccca tgcacatctc
      961 gtaccgaagc gccggatcgt cacggagagc ccgggcgacc tcgacgaggt gttctcggcg
     1021 gacgaagaag gtgatctctc cccggtcgac gaccaccttc tcgacggcgg cgtcgaacgg
     1081 aaggccggcc tgttcgagga ctccagtcaa cgaatcaccg acgtcatcga actccggtcc
     1141 ccacggccga ggtgttgact cgacgcggat cgggttggtg tcacgtgcga ccagccgccc
     1201 gaaacccgag gtgtcaccgg tgccggccgc accgaacatg ccacgtttgg tgggatgcgg
     1261 cggaggcaac ccggcggcgc cctcgtcggg cacctcctcc ggaaggtcac cggggccggg
     1321 ggtcgtcgcc tcggcactcg gccggtcgac ggtcgccgga gcgtcgggag gagacgtctt
     1381 ctctgcctca ccgcccacag gggcggtggc ctcgtcgtga gccggcgtgc gatcgtcgct
     1441 catcgcagca ggcccctcat ctcgtgggtc ggggcggcct cgagtgcgtg ctcttcggcc
     1501 tcggcctccg cccgcttgcg gtgcggtcca agcggcgtgt gcaggatctt gtcgtgcagc
     1561 ttgaggatcg cgtccatgag catctccgga cgcggcgggc agccgggcag atagatgtcg
     1621 accgggacga tgtggtcgac tccttgcacg atcgcgtagt tgttgaacat gccgcccgag
     1681 ctcgcgcaga cacccatcgc gatcacccac ttgggctcgg gcatctggtc atagatctgc
     1741 cgcagcaccg gcgccatctt ctggctcacc cggcccgcga cgatcatcag gtccgcctgg
     1801 cgcggggaag cgcggaagac ctccatcccg aaccgggccg agtcgtgccg cggcccgccg
     1861 aaggccatca tctcgatggc acagcaggcg agcccgaacg tcgctggcca caccgagctc
     1921 ttgcgcatga atccgaccaa gcgctcgacg ccggtcaaca ggacgccggc cggcagcttc
     1981 tcctcgatac ccatgctcga atcccgtcct agtcccagtc caacccgccg cgacgccaga
     2041 catatgcgta ggcaacgaac acggtgagaa tgaacaagac catc
//
LOCUS       DASTXO010000419         2447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_172080, whole genome shotgun sequence.
ACCESSION   DASTXO010000419 DASTXO010000000
VERSION     DASTXO010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172080"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..954)
                     /gene="sufC"
                     /locus_tag="VFU96_08785"
     CDS             complement(154..954)
                     /gene="sufC"
                     /locus_tag="VFU96_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019006341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEU5113400.1"
                     /translation="MTTALEVRGLSAAVDGKQILEGLDLVVPFGEVHALMGPNGSGKS
                     TLCHVLTGKAGYVVTGEALLEGHELLSLEVDERARLGLLQSFQYPTEVPGVALREFLL
                     EAAEERGISEADATDRIDREAARFDVTRFLDRSINNDLSGGEMKRSEIFQMAVLRPRV
                     ALLDEIDSGLDIDAVKQVARAVEEMRSPNLGVLMITHYNRILRYVTPDRIHVMIGGRI
                     VDSGGAELAVELESGGYKAIQERLGLAEATTEPSRRPADFFTDTPFDV"
     gene            complement(951..1262)
                     /locus_tag="VFU96_08790"
     CDS             complement(951..1262)
                     /locus_tag="VFU96_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEU5113401.1"
                     /translation="MRVEIGAVDTLPRDRGIRVSLGEDRIAMFRIGDEVFAVADRCSH
                     AEASLAEGEVFDHEVECPRHGSEFDLRTGKPLSLPATKPVPTYEVVIEDGVVYLMWEE
                     S"
     gene            complement(1259..>2447)
                     /gene="sufD"
                     /locus_tag="VFU96_08795"
     CDS             complement(1259..>2447)
                     /gene="sufD"
                     /locus_tag="VFU96_08795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEU5113402.1"
                     /translation="AAYEVFAALDEPTGAEEDWRYVDFDHSFSELAPVATAGAALEPG
                     PFVTSLPERSGRILIIDGEVVEHDSDVALVQRFSDLDSDPGFSGMVPTDHNKLAAAHA
                     AFSTDGARVELKSGTVMEAPLVVEIHATTEGTVSFPHLQVVVGENAEASILVDYRSAD
                     GARLLLVPRVDLEVGDSGRLRFLSVQGLDHAATTVVHQRAVLGRDSTSRIGEVGLGAR
                     LGRLDLGVSLVGDGSSSEVVGLYFGEGDQTLDYRMVIDHQGRSTTSDVFLKGAVEDDA
                     QSVFTGLLRIEKDAARTSTFETNRNLVLSENAKAHSVPNLEILCNDVICGHASSVGPL
                     ESEHLYYLMSRGLNRERAERLLIRGFFQQVIDRLPIEGLGEPVSDEVFRRFVAAQEEG
                     RVA"
BASE COUNT          454 a          807 c          760 g          426 t
ORIGIN      
        1 gagtccacag ccatcctcag gtcggagcgc cgatcgaccc aggcccggac ccatcggacc
       61 acttcgtgct cgtgacccgg ggccgtggga agatgggtca gggcgccgag ccatgtctcc
      121 gcagcggcac gggaccccat cagaatcggc ggttcacacg tcgaaggggg tgtcggtgaa
      181 gaagtctgcc ggccgtcgtg acggctcggt tgtggcctcc gccaatccca ggcgctcctg
      241 tatcgccttg tagcctccgg actccagttc gacagcgagc tcggccccgc cggagtcgac
      301 gatccgcccg ccaatcatga cgtggatccg gtcgggagtc acatagcgga ggatccggtt
      361 gtagtgggtg atcatcaaga ccccgaggtt gggcgaccgc atctcctcca ctgccctggc
      421 cacctgcttc accgcgtcga tgtcgagacc ggaatcgatc tcgtcgagaa gcgcgactct
      481 cggtctgagc accgccatct ggaagatctc agaccgtttc atctcgcccc cggagaggtc
      541 gttgttgatg gatcgatcga ggaacctggt cacatcaaaa cgtgcggctt cccgatcgat
      601 ccggtccgtg gcgtccgcct cggagatccc ccgctcctcc gcggcctcga ggaggaattc
      661 ccgaagggcg accccgggca cctcggtggg atactgaaag ctctggagca gcccgagtcg
      721 ggctcgctcg tccacctcga gggacaacag ttcgtggcct tcgaggagag cctccccggt
      781 caccacgtag ccggccttgc ctgtcaacac gtggcacagg gtggacttgc ccgatccgtt
      841 gggccccatc agggcgtgca cctcaccgaa cgggacgacc aggtcgaggc cctccaggat
      901 ctgcttaccg tcgacagcgg ccgacagacc gcggacctcg agcgccgtgg tcatgactcc
      961 tcccacatga ggtaaacgac cccatcctcg atgacgacct cgtaggtcgg caccggcttt
     1021 gtagccggca acgacaaagg cttgccggtc cgcagatcga actcggaacc atgtcgtggg
     1081 cactcgactt catgatcgaa cacctcccct tcggcgagag atgcttccgc atgcgagcaa
     1141 cggtcggcga cggcgaacac ctcgtcgccg atgcgaaaca tggctatgcg atcctcaccc
     1201 aggctgaccc ggatgccacg atcgcggggc aaggtgtcga cagcgccgat ctcgaccctc
     1261 atgcgacccg gccttcctcc tgtgccgcga cgaaccgtcg gaagacctcg tcggacacgg
     1321 gctccccgag accttcgatc ggcaatcggt cgatcacttg ctggaagaac cctcggatca
     1381 ggagtctctc cgctcgttcc cggttcagcc cccggctcat caggtagtag aggtgctccg
     1441 attccaacgg gcccaccgat gaggcgtgac cgcatatcac gtcgttgcag aggatctcga
     1501 ggttgggcac cgagtgtgct ttggcgttct ctgagagaac gagattgcga ttggtctcga
     1561 aagtcgacgt tcgggcggca tccttctcga tccggagcag gccggtgaag acactctgag
     1621 cgtcgtcctc gaccgcacct ttgaggaaga cgtcggatgt tgtgctccgt ccctgatgat
     1681 cgatcaccat tcggtagtcg agggtttgat ccccctctcc aaagtagaga ccgaccacct
     1741 ccgatgagga gccgtccccg acgaggctta cgccgagatc gagccggccg agcctggcgc
     1801 ccagccctac ctcaccgatg cgggacgtgg aatcgcgtcc cagcaccgct ctctgatgca
     1861 ccaccgtggt cgcggcgtga tcgaggccct ggaccgagag aaatcgcaac cgtccactgt
     1921 cccccacctc gaggtcgacc cggggtacga gaagcagtcg ggcgccatcg gccgagcggt
     1981 agtccacgag gatgctggct tcggcgttct cgcccaccac gacctggagg tgcggaaaag
     2041 aaacggtgcc ctcagtggtg gcgtggatct cgaccaccaa aggggcttcc atgaccgtgc
     2101 cggacttcag ttcgacccga gccccgtcgg tggagaatgc ggcgtgggcc gcggccagtt
     2161 tgttgtgatc agtcgggacc atccccgaga agcctggatc ggaatcgagg tcggagaagc
     2221 gctgaacgag ggcgacatca gagtcgtgct cgacgacctc cccgtcgatg atcaggatgc
     2281 gtccggaacg ctccgggagg gatgtgacga acgggccagg ctcgagcgcc gcaccagcag
     2341 tggcgaccgg cgctagctcg gaaaagctgt ggtcgaaatc gacataccgc cagtcttcct
     2401 ccgcccctgt gggctcgtct agtgcagcga aaacctcgta cgcagcc
//
LOCUS       DASTXO010000420         2074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_651307, whole genome shotgun sequence.
ACCESSION   DASTXO010000420 DASTXO010000000
VERSION     DASTXO010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651307"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VFU96_08800"
     CDS             complement(<1..571)
                     /locus_tag="VFU96_08800"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEU5113403.1"
                     /translation="MIEELDGIGDRLRAERTKAGISQRELARRLGLSASLISQLESGL
                     SKPSVGTLYAIVTELDLSLDKLLRGDENAVRDDGESGLPGQNGRGGPLVHPDRRKVID
                     LASGVRWEQLTADTEEGVDFLHAIYEVGGASTPDESLMRHHGREYGYVISGKMGIQLG
                     FELFELEPGDSIAFDSTEPHRLFNMGDEPV"
     gene            complement(593..1285)
                     /locus_tag="VFU96_08805"
     CDS             complement(593..1285)
                     /locus_tag="VFU96_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004923036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5113404.1"
                     /translation="MADGTVLIVGGTGGLGRELAAHYASQGREVIITGRDEGKAKEIA
                     SGLGSNARAIGFDLAAPETIADALRDVGEVDKLVLSAIARDNNPVREYDISSAVTLAT
                     MKVVGYTEVIHTLIGRLSDDSSVVLFGGRAKDRPYPGSTTVTAVNGAVATMITAFAIE
                     LAPIRFNAIHPGIVGDSPFWEGKPLESVLARTPTKRLATMEDVVEATVFLLENRSVNG
                     INLYVDSGWLMM"
     gene            1422..>2074
                     /locus_tag="VFU96_08810"
     CDS             1422..>2074
                     /locus_tag="VFU96_08810"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5113405.1"
                     /translation="MAYDVCIIGAGLVGLGVARALTETHTGIGIVILDKEPSVAAHQS
                     SHNSGVVHSGLYYRPGSLKARLCVEGRRMLKDYCEATGLAFEESGKLVIATKPSELEP
                     LAELERRGTANGLSGLTRLGGQEIRQLEPEATGLEALHVPEAGVVDFPAVAARLAGDL
                     VADGADLRTDFEVAAISHHPDGADIRSADNEIQARVVVNCAGLHSDRVAALAGVRSQV
                     "
BASE COUNT          364 a          676 c          655 g          379 t
ORIGIN      
        1 gcaccggctc gtcgcccatg ttgaagagac gatggggctc cgtcgagtcg aaggcgatgg
       61 agtctccggg ctcgagttcg aacagctcga aaccgagctg gatccccatc ttccctgaga
      121 tcacgtagcc gtactcccgt ccatgatgcc tcatcagcga ctcgtccggc gtcgacgcgc
      181 cacccacctc gtagatggca tggaggaaat ccaccccctc ctcggtgtcc gctgtgagct
      241 gctcccaacg gactcccgag gcgagatcga tcaccttgcg cctgtcgggg tggaccagtg
      301 ggcctccccg gccgttctga cccggcagac ctgattcgcc gtcgtctcga acagcgttct
      361 cgtcgccacg aagaagcttg tccagggaga gatccagttc ggtcacgatg gcgtagagcg
      421 tccccaccga cggcttggag agcccgctct ccagttggga gatgaggctg gccgacagac
      481 cgaggcgacg cgccaattcc ctttgcgaga tcccggcctt ggtccgctcc gcgcggagtc
      541 gatcacctat tccgtcgagt tcctcgatca tctccagagc atcctcatct ggctacatca
      601 tcagccagcc gctatcgaca tagagattga tcccgttcac cgatcggttc tccaggagga
      661 agacagtggc ctcgaccacg tcctccatcg tggcaaggcg cttggtcggc gtccgcgcca
      721 aaaccgattc gagaggcttg ccctcccaga aggggctgtc gccaacgatg ccgggatgaa
      781 tcgcgttgaa cctgatgggc gcgagttcga tcgcgaaggc ggtgatcatg gtggccacgg
      841 ctccgttcac ggcggtgacc gttgtcgacc cgggatacgg gcggtctttc gccctcccgc
      901 caaagagcac cacggagctg tcgtccgaca gccttccgat gagagtgtgg atgacctcgg
      961 tgtaacccac caccttcatg gtggccagcg tcacagcact gctgatgtcg tactcacgga
     1021 ccgggttgtt gtcgcgggcg atggcactca gcaccagctt gtccacctcc ccgacatccc
     1081 ggagggcgtc ggcaatggtc tccggggcgg cgaggtcgaa cccgatagcc ctggcattgc
     1141 tacccagccc cgaggcgatc tccttcgctt tgccctcgtc ccggccggtg atgatcacct
     1201 cgcgtccctg acttgcatag tgggccgcaa gctcgcggcc caagccaccg gtgccgccaa
     1261 cgatcaacac tgtcccatcg gccatcgatc agctcctcat ggtggcgaag tgcggagatc
     1321 ctaacgctcc ggcaggcccg actgtgcagt gagactgagc accactgcac ttcgtggtta
     1381 gtctgcgcca ctcgctcgtg gctgtgtgag gaggatgccg ggtggcttac gacgtctgca
     1441 tcatcggcgc cggtctggtc ggcctcggcg tcgcccgagc cctcactgag acccatacag
     1501 gcatcggcat cgtcatcctc gacaaagagc cgtcggtggc ggcacaccag agcagtcaca
     1561 acagcggcgt cgtccattcc gggctctact accggccagg ctcgctcaag gcgcgtctct
     1621 gtgtggaggg gagacgcatg ctcaaggact actgcgaggc tacggggctc gccttcgagg
     1681 agtcgggaaa actggtgatc gccaccaagc cgtccgagct ggagcccctg gccgaattgg
     1741 agcgacgagg gacggccaac ggcctctcgg gactgacccg gctgggaggc caggagattc
     1801 gacagctcga acccgaagcg acagggctcg aagcacttca cgtcccggag gcaggggtgg
     1861 tggacttccc ggccgtggcg gcaaggctgg ccggcgatct ggtagccgat ggtgccgacc
     1921 tccgcaccga tttcgaggtg gcggcgatat ctcatcatcc ggacggggcc gacattcgtt
     1981 cggcggataa tgagattcag gccagggtgg tggtgaactg cgccggtctc cactccgatc
     2041 gggtggcggc cctggccggg gtgcgaagtc aggt
//
LOCUS       DASTXO010000421         2044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_430534, whole genome shotgun sequence.
ACCESSION   DASTXO010000421 DASTXO010000000
VERSION     DASTXO010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430534"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="VFU96_08815"
     CDS             complement(<1..733)
                     /locus_tag="VFU96_08815"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEU5113406.1"
                     /translation="MTTGARAMAEMLQGYGVTHLFHVPAVLRHTMVELEQHTSIKRLR
                     PHGEASAAYMADGYARASGRPAVCAAQVVGAHNLAAGLRDAFLAHSPVIALTGGRDPH
                     TKFRKVYQEVDDVPAFETVTKMNATVDSVERIPDMLRQAFRVATTGAPGPVHLQFAGN
                     EGQLDAGEAEMDVSCETRYSSVPPFRPIAPDADLNGALDLLGTAERPVIVAGGGVRTS
                     GAGAALVALAERLSIPVATSLNGKDT"
     gene            complement(733..1131)
                     /locus_tag="VFU96_08820"
     CDS             complement(733..1131)
                     /locus_tag="VFU96_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113407.1"
                     /translation="MSAPPAVGAEAPIQVRTPSALDLFMFSASAWLLHRIHYDTPFTV
                     EHDGHPGLLIHGPLQGVYLVQTAESWLGDDARLATITYRHLTPAYLGDSLECGGTVTG
                     SDSDGIELELWARKPDGTVTTSGTARFVTT"
     gene            complement(1128..1616)
                     /locus_tag="VFU96_08825"
     CDS             complement(1128..1616)
                     /locus_tag="VFU96_08825"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEU5113408.1"
                     /translation="MGVITEEARRWADRDYPRFEMQVTATDIARFAHATGETDPIHFD
                     RETAIAAGYADVVAPTLFSYVIRMHASALVPPDQLELDGSPKADVPPLPTRRAMAGET
                     SVALGERVVAGDVITVEKRLARMYEKEGRSGPLVFVEMEFTFTNQRGELVAKENFTRI
                     YR"
     gene            complement(1616..>2044)
                     /locus_tag="VFU96_08830"
     CDS             complement(1616..>2044)
                     /locus_tag="VFU96_08830"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5113409.1"
                     /translation="YLRYERLCLSAARTGAAAAALADSVAYAKEREQFGQPISKFQAV
                     SHRLADMQTMVEISQMLVYRYASRLDRGLATTRDAAILKLYACEAYKQVADMGLQVLG
                     GYGYTMEYDLQRHFRESRLGVIGAGTSDIQRNIIAKTMGL"
BASE COUNT          336 a          630 c          712 g          366 t
ORIGIN      
        1 tggtgtcctt cccgttgagc gacgtggcca ccggtatcga caaccgctcg gcaagagcaa
       61 ccagcgccgc gccggctccc gaggtccgga ccccgccacc ggcgacgatc accggtcgtt
      121 ctgctgtacc gagcagatcg agggcaccgt tcagatcggc gtccggcgcg atgggtcgaa
      181 acggaggcac tgaggaataa cgcgtctcac aggagacgtc catctccgct tccccggcgt
      241 cgagctgacc ttcgttcccg gcgaactgga gatggaccgg gcccggggcg ccggtcgtgg
      301 ccaccctgaa ggcctggcgc agcatgtcgg ggatccgctc gacggaatcg accgtggcgt
      361 tcatcttggt cacggtctcg aaggcgggga cgtcgtcgac ctcttgatac accttgcgga
      421 acttggtgtg cggatcccgg cctcccgtca gggcgatgac cggggagtgg gcgaggaaag
      481 cgtcgcggag accggctgcc aggttgtggg cccccaccac ctgggcggcg cagacggcgg
      541 gacgtcctga cgctctggcg taaccgtcgg ccatgtaggc cgctgatgcc tccccgtggg
      601 gtcggagacg cttgatcgag gtgtgctgct ccagctcgac catcgtgtgg cgaagtaccg
      661 cagggacgtg gaagagatgg gtgaccccgt acccctggag catctcggcc atggctcttg
      721 cgccagtggt catcatgtgg tgacgaacct cgctgtcccg ctggtggtca ctgtgccgtc
      781 cggctttcgg gcccaaagtt cgagctcgat gccgtcggaa tcgctgccgg tcaccgtgcc
      841 gccgcattcc agcgaatcgc ccaagtatgc gggggtgaga tgccggtagg tgatagttgc
      901 gagacgtgca tcgtctccga gccacgattc cgccgtctgc accaggtata cgccctgcaa
      961 cgggccgtgg atcagtaggc cagggtgacc gtcgtgctcg acggtgaacg gggtgtcgta
     1021 gtggatgcga tgaaggagcc aggctgaggc gctgaacatg aacaggtcca gcgccgatgg
     1081 agtacggacc tgaatcggcg cctcggcgcc cacggctggc ggggcgctca ccgatagatc
     1141 ctggtgaagt tctccttcgc caccagctcg ccccgctggt tggtgaaggt gaactccatc
     1201 tcgacgaata cgagcgggcc ggatcgcccc tccttctcat acatccttgc cagccgtttc
     1261 tcgacggtga tcacgtcgcc ggcgacgacc cgttcaccga gtgcgaccga tgtctccccg
     1321 gccatcgcgc ggcgggtggg gagcggtggg acgtcggcct tcggggaccc gtccaattcc
     1381 aactggtcag gcgggaccag ggccgaggca tgcatccgaa tcacgtagga gaacagcgtg
     1441 ggcgcgacca cgtcggcata cccggcggcg atggcagtct ctctgtcgaa atggatcgga
     1501 tcggtctcgc cggtggcgtg ggcgaaccgg gcaatgtcgg tggcggtcac ctgcatctcg
     1561 aagcgggggt aatcccggtc ggcccagcgg cgggcttcct cggtgatgac tcccatcaga
     1621 gtcccatggt cttggcgatg atgttgcgtt ggatgtcgga ggtgccggcg ccgatcactc
     1681 caagacgtga ctcccggaag tggcgttgca ggtcgtattc catcgtgtat ccgtagccgc
     1741 cgagcacttg gagacccatg tcggcgacct gcttgtaggc ctcgcaggcg tagagcttga
     1801 ggatcgccgc gtccctggtg gtggcgagac cccggtcgag tcgtgaggcg tagcggtaga
     1861 ccagcatttg tgagatctcg accatggtct gcatgtcggc gagacggtgc gagacggcct
     1921 ggaacttcga gatcggctgc ccgaactgct cacgttcctt ggcgtaggcg accgaatcgg
     1981 cgagggcggc ggcggcggcc ccggtccgag ccgccgagag acagagccgc tcatagcgga
     2041 gata
//
LOCUS       DASTXO010000422         4665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_725379, whole genome shotgun sequence.
ACCESSION   DASTXO010000422 DASTXO010000000
VERSION     DASTXO010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725379"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VFU96_08835"
     CDS             <1..353
                     /locus_tag="VFU96_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113410.1"
                     /translation="VSGWFPDGIGGGPRGSELPIRMLREAVRRLRPGGRVFLPTGSLQ
                     DETAILEAARGLYSKVAALAEKMIPLPSLLADSETLKNLIADGIVHIVPKGSRFLWET
                     RVWRLSGLTSGESS"
     gene            389..1123
                     /locus_tag="VFU96_08840"
     CDS             389..1123
                     /locus_tag="VFU96_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017592171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEU5113411.1"
                     /translation="MAGPGPTEELASKDAYLAQSEGSVVSVSDDGLVLDRTVFYARGG
                     GQPGDLGVIRWDGGQVDVIDTIRRDGMPFHVVAAGVALPDPGTPVHGLIDWERRYRTM
                     RTHTALHALSGVIFRDFGAKVTGGNMEPGVARMDFELDAISVEFGQEVERRLNEELVR
                     GYPTEVVFMARQEALKDPDLIRTKVNLIPEWVEEIRVIDIVGLDRQADGGTHVASTLE
                     VGQVQVVKTESKGKANKRMRIELSGS"
     gene            1152..1547
                     /locus_tag="VFU96_08845"
     CDS             1152..1547
                     /locus_tag="VFU96_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5113412.1"
                     /translation="MSVIVGYMSAERGRAALELGISEAKLRNTDLVVVHSLHGAGVDD
                     DEDVVASDRELEIIDEELSKEGITYSIHNFVRGNAPADDIVQAAGDLGGELIVIGLRQ
                     RTSAGKFLLGSNAHDILMSAPCPVLTIRV"
     gene            1604..2566
                     /locus_tag="VFU96_08850"
     CDS             1604..2566
                     /locus_tag="VFU96_08850"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEU5113413.1"
                     /translation="MIDFILGLFIAGLLVRGWLRGFVREALDLVALVIGLWVAFRLSG
                     PLGEFITERFSVAPEVATIGAGIVLFLLFGVAMSIAAHYLSRVMSLPGLNLINRLGGA
                     AVAALWGIAIILVVINLARVVPGGWDGRFEDSSVAQAIAGPDAAPQEFFETMAPDGVL
                     GSLAAIQDLFGVNRVVPEGNEVLAIPPAAPDEVRQVREETAGVLARVNEHRTGLGVPA
                     LGRSASLDGVAESRAVGMYTSGRLSRDYPPGGDVAADVAAAGIRLEIVGENLALASSA
                     RAAVDAMLGSPTALAQFAISAYDRTGISVVDGPTGRLVVIVMGG"
     gene            complement(2576..3463)
                     /locus_tag="VFU96_08855"
     CDS             complement(2576..3463)
                     /locus_tag="VFU96_08855"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU5113414.1"
                     /translation="MTLLAALAGAVAISFSAILYALAEVSPITGGFFRVFYALPILFV
                     LWWLRRRDDHRTRNKRLVAIVAGLFLGLDILVWHISIEYIGAGLSTLIANSQVIVVAV
                     IAWIFLGERPSRQVAIAVPIVLLGVSLVSGLGRGDAFGSNPLLGTILALASALLYAGF
                     ILGFRISNDIQAPSAGPLLESTAGAGIALLLAGIVSGGIDLVPTWPAHGWLLVLALTS
                     QVAGWLLIGYALPRLPAVETATIILLQPVLTMIWAVLIFSESPSTIQWVGAALVLSGV
                     GFVAVSRARRTPVIAPAQP"
     gene            complement(3460..3855)
                     /locus_tag="VFU96_08860"
     CDS             complement(3460..3855)
                     /locus_tag="VFU96_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113415.1"
                     /translation="MSNLDLADGLRMMQQAGEGGYFGHEAHLRFAWSVLDETDDLDDA
                     ERLACLTIRHAADIGGNPAKYHATVTMFWIRLVEHLKQAHPDVTSVEQMLHVYPPLGD
                     SSLPQRHWSNLDSEESRTSWVDPDLIPLP"
     gene            complement(3852..>4665)
                     /locus_tag="VFU96_08865"
     CDS             complement(3852..>4665)
                     /locus_tag="VFU96_08865"
                     /inference="COORDINATES: protein motif:HMM:NF018657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HEU5113416.1"
                     /translation="RLSHLSEAVRDGVMLTDRTLDKSAAAVSSLRSGWLRAADRTPLN
                     PDIGPNRRFDWTDTPLEAIKDLKNKLGGSVNDVVLAITAGAVRQFLLDERGYDVSGAE
                     FRVMNPVSTRSPDQRGALGNQLAMWLLDLPIQEPDPRVRFERVKEATSNLKKTNQALG
                     AATLVEISSGTPITLLSLANRVVGQRIRPFNMTVTNIPGPQFPMYLLESEMIANYPMV
                     PLWAQHGIGIALFSYNGRLLWGIHADYDTLPDSDRFLAAIQDSLRELQNLPS"
BASE COUNT          843 a         1388 c         1628 g          806 t
ORIGIN      
        1 aggtcagcgg ctggtttcct gacggcatag gcggagggcc acggggcagt gagttgccga
       61 tccgtatgtt gcgtgaggcg gtgcgccggt tgcggcctgg aggccgggtc tttctcccca
      121 ccgggagtct ccaggacgag actgccattc tcgaggcggc ccgtgggttg tatagcaagg
      181 tggcggccct cgccgagaag atgatcccgc ttccttcgct gctcgccgat agcgagacgc
      241 tgaaaaacct gattgccgac ggcatcgtcc atatcgtgcc caagggctcc aggttcctat
      301 gggagacgag ggtttggagg ctctctggtc tgacgtccgg agagtcttcc tgaggagggc
      361 gcgccccggg cccacctagt ctgccgcgat ggccgggccg ggacccacgg aagagctggc
      421 gtcgaaggac gcatacctgg cccagtccga gggatcggtc gtgagcgtgt cagacgacgg
      481 cctcgtcctt gatcgcaccg tgttctacgc ccgtggcggc ggccaaccgg gagacctcgg
      541 ggtgattcgc tgggacggcg gacaggtcga tgtcatcgat accattcgga gggacgggat
      601 gccgttccac gtcgttgcag ctggtgtcgc cctccctgat cccgggacac cggtccacgg
      661 cctgatcgat tgggaacgtc gctatcggac gatgaggacg cacaccgccc tccacgctct
      721 ctccggcgtg attttccggg acttcggggc caaggtgacc ggaggaaaca tggaacccgg
      781 cgtcgcccgc atggacttcg agctcgacgc catctcggtc gaattcggcc aggaggtcga
      841 gcggaggctc aacgaggaac tggtccgcgg ctatccgacc gaggtcgtgt tcatggcccg
      901 ccaggaggcg ttgaaggacc ccgacctcat tcggaccaag gtcaatctca tcccggagtg
      961 ggtggaggag atcagggtga tcgacatcgt tggcctcgac aggcaagccg acggggggac
     1021 ccatgtggct tcgaccctgg aagtgggaca ggtgcaggtc gtcaagaccg agtcgaaagg
     1081 gaaagcgaac aagaggatgc ggatcgagct ctccgggagc taatttctgc caccagaggc
     1141 gaaaggcaac catgagcgtg atcgtcgggt acatgtccgc cgagcggggc cgggcggcgc
     1201 tggagttggg gatctccgag gcgaagcttc ggaataccga cctggtggtg gttcattccc
     1261 tgcacggcgc aggagtcgac gacgatgagg atgtggtggc cagcgatcgg gagctagaga
     1321 tcatcgacga ggagttgtcg aaggaaggca tcacctactc gatccacaac ttcgtgaggg
     1381 gcaacgcacc cgcagatgac atcgtccagg ccgcaggtga cctcggcggc gaactgatcg
     1441 tcatcgggct caggcaacgg acatcggccg ggaagttcct cctcggctcc aatgcccatg
     1501 acatcctgat gtctgctccc tgtccggttc tgaccatcag ggtgtgaaac ctggaagcgg
     1561 aaagccgctt ccagccacct gatacctgtt acttactacc ttcgtgatcg acttcatcct
     1621 cgggttgttc atcgcgggtc tgctagtccg gggctggctc cgtggattcg tccgggaagc
     1681 gctcgatctg gtggccctcg tcatcggcct ctgggtggcc ttccgcctga gcggcccgct
     1741 cggggagttc ataaccgagc gcttctcggt ggcgccggag gtggccacga tcggggcggg
     1801 aatcgtcctc tttctccttt ttggtgtcgc gatgagcatt gctgcccact atctgtcacg
     1861 ggtgatgtcc ctaccggggc tgaatctcat caaccgtctg ggcggcgccg cagtcgctgc
     1921 gctttgggga atcgcaatca tcctcgtcgt gatcaatctg gcccgtgtgg tcccgggtgg
     1981 atgggacggc cgcttcgagg actcttccgt ggcccaagcc atcgccgggc cggacgcggc
     2041 gccacaggaa ttcttcgaga cgatggcgcc cgacggcgtg ctcggctcgc tggctgccat
     2101 ccaggatttg ttcggggtca accgggtggt cccggagggc aacgaggtcc tggccatacc
     2161 ccccgccgcg cccgatgagg tccgacaggt gagagaagag accgccgggg tcctggcccg
     2221 ggtgaacgag caccggacgg gtctcggtgt accggcgctc gggcgcagcg ccagtctgga
     2281 cggcgtcgcc gaatcgagag cggttgggat gtacacgagc ggccggctct cccgtgacta
     2341 cccgcccggt ggtgacgtgg cagccgatgt tgccgcggcc gggatccgcc tggaaatcgt
     2401 cggggagaac ctggccttgg ccagcagcgc ccgagcggcg gtcgacgcca tgctcggatc
     2461 gccaacggcc ctcgcccagt tcgccatatc cgcctacgac cgcaccggga tctcggtggt
     2521 cgacggcccc accggccgtc tggtcgtcat agtgatgggg ggctgattcc cgccttcagg
     2581 gttgtgctgg agcgatgacc ggggtgcggc gggcacggga gacggcgacg aagccaaccc
     2641 cggagaggac gagggcggcg ccaacccact ggatggtcga cgggctctcg gagaagatga
     2701 gaacggccca gatcatggtc aggaccggct ggaggaggat gatggtggcg gtctcgacgg
     2761 caggaagccg gggtagcgcg tatccgatca gcagccaccc ggccacctgg gaggtgaggg
     2821 ctagaacgag gagccaacca tgggcaggcc aggtcgggac caggtcgatg ccgccgctca
     2881 cgatgccggc aaggagaagg gcaatgcctg cgcccgccgt ggattccagg agcggccctg
     2941 ccgatggagc ctggatgtcg tttgagatcc ggaaaccgag gatgaacccg gcgtagagca
     3001 atgcggaggc caaggcgagg atcgtcccga gcagcgggtt ggagccgaac gcatcccccc
     3061 ggccgagacc gctgacgagg cttactccga gcagaacgat cggcacggcg atcgccactt
     3121 gtcggctcgg acgctcgccg aggaagatcc aggcgataac cgcgaccacg atcacctggg
     3181 agttggcgat gagcgtcgaa agaccggcgc cgatgtattc gatcgagatg tgccagacca
     3241 ggatgtcgag cccgaggaag agcccggcga cgattgccac caagcgcttg ttccgggttc
     3301 gatggtcgtc ccggcgtcgc aaccaccaca ggacgaacag gatcggcagc gcatagaaga
     3361 cccggaagaa gccgccggtg atgggggata cctcggcgag tgcgtacagg atcgcagaga
     3421 aagagatggc cacggcgccg gccagcgcgg caagcagcgt cacggaagcg ggatcagatc
     3481 cggatcaacc caggatgtcc tcgactcctc gctgtcgagg ttcgaccaat gtctttgcgg
     3541 tagcgaagag tcaccgagtg gcgggtacac gtggagcatt tgttccacgg acgttacgtc
     3601 gggatgggct tgtttcaaat gttcgacgag ccggatccag aacatggtca cggtggcgtg
     3661 atacttcgcc gggttgccgc cgatgtcggc tgcatgccga atcgtcaggc aggcgagccg
     3721 ttcggcgtcg tcaaggtcgt cggtctcatc gagcaccgac caggcgaagc gaagatgagc
     3781 ctcgtgcccg aagtagccac cctcgcccgc ctgctgcatc atccgcagcc cgtcggcgag
     3841 atcgaggttg ctcacgaggg caggttctga agctcccgga gcgagtcctg gatggcggca
     3901 agaaagcggt cggaatcggg cagcgtgtcg tagtcggcat gaatgcccca gagcagccgg
     3961 ccgttgtacg agaagagggc gatcccgatc ccgtgttggg cccagagcgg caccatcggg
     4021 tagttggcga tcatctccga ctccaacagg tacatcggga actgcggtcc cgggatattg
     4081 gtgaccgtca tgttgaacgg ccggattctc tggccgacca cccgattggc cagggagagc
     4141 agagtgatcg gggtgcccga gctgatctcg accagggttg ctgctccgag ggcctggttc
     4201 gtcttcttca ggttgctcgt tgcctccttg accctctcga atcgaacccg cgggtcgggc
     4261 tcctgaatag ggaggtcgag gagccacatc gccagctggt tgccgagggc cccgcgctgg
     4321 tcggggctgc gagtggagac cgggttcatc accctgaact cggcgccact gacgtcgtag
     4381 ccacgctcgt cgaggaggaa ttggcgcacc gcgcctgcgg tgatggcgag caccacgtca
     4441 ttgacagatc caccgagttt gttcttcaga tccttgatcg cctcgagagg cgtgtcagtc
     4501 cagtcgaatc gccggttcgg accgatgtca gggttgagcg gggtccggtc ggcggcacgc
     4561 agccagccgg agcggagcga agaaaccgcg gcagccgatt tgtcgagcgt gcgatcggtg
     4621 agcatgacgc cgtcacggac cgcctccgag agatgagaga gccgg
//
LOCUS       DASTXO010000423         3453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1830281, whole genome shotgun sequence.
ACCESSION   DASTXO010000423 DASTXO010000000
VERSION     DASTXO010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1830281"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VFU96_08870"
     CDS             complement(<1..422)
                     /locus_tag="VFU96_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5113417.1"
                     /translation="MIVFNGNAGNRSHRAPLARALNDQGLAVLLTDYRGFGDNPGSPS
                     EEGLALDASAALAYLETRPDVDTDRLVYFGESLGAAVALRLDFARPPAALVLRSPFTS
                     LAEVGAAHYPLLPVSWLLRDRFENTDLISQSDTPLLIIL"
     gene            complement(419..640)
                     /locus_tag="VFU96_08875"
     CDS             complement(419..640)
                     /locus_tag="VFU96_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113418.1"
                     /translation="MARMKALAVVVALAIVGVGAIWLFQRSLIYLPIDSVSPIEAVLP
                     GGEEVTFPTADGLTSPAGICRRRPLRRQR"
     gene            718..2892
                     /locus_tag="VFU96_08880"
     CDS             718..2892
                     /locus_tag="VFU96_08880"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEU5113419.1"
                     /translation="MLENRHETRGRWAGLWGDFSLDRLPATLVTGLVLAVVNSLLCIA
                     LMTLIFDGPLDEALPIGIGLGLAASAVVAMVLAIGSSFPGMYGGVQDASAAILGLSAA
                     SIAASISRPEAVDTVLAMMVVTSLATGLVFLLMGYFGLGEIARFVPFPVIGGLLAGTG
                     YLILVGAVGILGAQSAGDLMSSDALGLFWPGVILAALFSIALHRRWPSWVYLVFLAGG
                     IIGFHAVTQISGVDMAESLNRGWLLGPFPEGGLWPGPALDALAGADWSAIAGEAASLA
                     TILLVVPISLLLYISALEVETKLDIDMNAELRATGWANVAGGAVGGPPGYFYLADTLI
                     TQRLVGRRRGPAVVAALFILGVVTVGSSILEFVPLFIVGGLLLFVGTNFLVEWLWTSR
                     RRMTRPDYALVWGIVVIIATVGFLPGVAAGLVAAIALFVVRYSRIDVVKHSLTAKEHQ
                     SNIERSSIEAEYLHEAGDSVLILELQGFIFFGTASRIIGHVRSHLESSEALRFVIFDF
                     RLVTGVDSSAVVLFERVALLAVDQGLVLLLTGLGPAQRAQFADLISDYDEVVRAEPDL
                     DHGMAWCEDRLLATVGPGQGQEQSLPRGLTDALAPFLAARTIPAGQELMRQGDPTPGI
                     FLILSGRVTVLLEGTDGQRVRLRTLLGGTVLGEISLYRDEPCSATAITETECEVLHLT
                     PEAFNELCRTDPEAAARFHVFVARTLAGRVSYANRAIRALHG"
     gene            complement(2915..>3453)
                     /locus_tag="VFU96_08885"
     CDS             complement(2915..>3453)
                     /locus_tag="VFU96_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU5113420.1"
                     /translation="FRFGGRGLLLGLFAFGLLMPIQSYFIAQNSLFEFLNLKDTRLAL
                     MIPYAAMGLSLAVYLLKVYLDSLPKELFEAARVDGCSDVRMFTTLVLPLLRPGLATVA
                     IFSALSAWNEFLLALLYITDDALKTIPTGLLAFSSRYLTDYQLLFAALTIITLPMVAV
                     YVIFHRQIIAGVTEGSLK"
BASE COUNT          596 a         1065 c         1123 g          669 t
ORIGIN      
        1 aggatgatca acagcggagt gtccgactgg ctgatgaggt ccgtgttctc gaagcgatca
       61 cggagcaacc acgagaccgg gaggagggga tagtgcgcgg ctccgacctc ggccagcgac
      121 gtgaacggcg agcggagcac caaggctgcc ggtgggcggg cgaagtcgag tcgcagcgcc
      181 accgccgcgc ccagcgactc tccgaagtag acgagacgat ccgtgtcgac atcgggcctg
      241 gtctccaaat aagccagtgc ggcggaggcg tcgagggcga gaccttcctc acttggtgat
      301 cccgggttgt ctccgaagcc ccgatagtcg gtgaggagca ccgccaatcc ctgatcattc
      361 agcgctcggg cgagcggtgc acgatgagaa cggttgcctg cattgccgtt gaacacgatc
      421 accgttgtcg cctcagcggc cggcgccggc agataccagc cggtgaggtg agcccgtccg
      481 ccgttgggaa agtgacctcc tcacctccgg gtagcacggc ctcgatcggg gacaccgagt
      541 cgatcggcaa atagatgagg ctgcgttgga aaagccagat ggcccccacc cccacaattg
      601 ccagtgcgac gacgacagct agcgccttca ttcgcgccac gatgccactg attcccagtg
      661 ggcgggagtg gtctcgcctg tggcgttgcc agctacctgc gatgtaatac tcaaacgatg
      721 cttgaaaatc gccacgaaac acgaggccgg tgggccggcc tctggggcga cttcagcctc
      781 gatcgtctcc cggcgacctt ggtcacaggg ctggtgctcg cagtggtcaa ctcgctgctc
      841 tgcatcgcgc tgatgaccct gatcttcgac ggaccgctcg acgaagcact tcccataggt
      901 atcgggttgg ggttggcggc ctcggcggtg gtagcgatgg tgctcgccat cggcagcagt
      961 tttcccggca tgtacggggg cgttcaggat gccagcgcgg cgatcctcgg gctcagcgca
     1021 gcttccatcg cggcctcgat ctctcgtccg gaggcggtcg acacggtgct cgccatgatg
     1081 gtcgtgactt cgttggccac tggcctggtc ttcctgctga tgggttactt cggtctgggc
     1141 gagattgcca gattcgtccc gtttcccgtc atcggcggtc tcctcgccgg cacgggctac
     1201 ttgatattgg tcggcgctgt tgggatcctc ggcgctcagt cggccggcga ccttatgagc
     1261 agcgacgctc ttggcttgtt ttggccgggt gtcatcctgg ccgcactgtt ctccatcgcc
     1321 ttgcatcggc gatggccgtc atgggtctat ctcgtgttcc tggccggtgg catcatcggt
     1381 ttccacgccg tcacgcagat ctccggcgtt gacatggccg aatcactgaa tcggggatgg
     1441 ctgctcggcc cgttcccgga gggcggtctg tggccggggc cggcactcga cgctctcgcc
     1501 ggcgccgact ggagtgcaat cgctggcgag gcagcaagcc tcgccaccat cctcctcgtc
     1561 gtcccgatat ctcttcttct ctacataagc gcgctcgaag tcgagacgaa gttggacatc
     1621 gacatgaatg cggaattgcg ggctacgggt tgggcgaacg tggccggcgg agcggtgggt
     1681 ggcccgccag ggtatttcta tctggcggac acattgatca cacagcggct cgttggccga
     1741 agacgcggcc ccgccgtcgt agccgcactg ttcattctcg gcgtggtgac ggtgggcagc
     1801 tcgatcctcg agttcgtgcc cctgttcatc gtgggtggcc tgctcctgtt cgttggcacc
     1861 aatttcctgg tcgaatggtt gtggacctcc cgccgaagga tgacgcgccc cgactacgcc
     1921 ctggtgtggg ggatcgtcgt catcatcgcc acggtcggct ttctcccggg tgtcgccgcc
     1981 ggattggtgg cagcgatcgc cttgttcgtc gtccgctaca gccggatcga cgtcgtcaag
     2041 cactcgttga ccgccaaaga gcaccagagc aacatcgaac ggtccagcat cgaggccgaa
     2101 tacctccacg aagcgggcga ttcggtcctg atactggagc ttcagggctt catcttcttc
     2161 ggaaccgcta gccggatcat cggacacgtc cgatcccact tggagagctc ggaggcgttg
     2221 cggttcgtca tcttcgactt caggttggtc accggcgtcg attcctccgc agtggtgctg
     2281 ttcgagagag tggccctgct cgcggtggat caaggcttgg tcttgcttct caccgggctc
     2341 ggtccggccc agcgggcaca attcgccgac ctgatctccg attacgacga ggtggtcagg
     2401 gccgagcccg acctcgatca cggaatggcg tggtgcgagg atcgtctgct cgccacggtt
     2461 gggccggggc agggacagga gcaatcgctg ccccgtgggc taaccgatgc gctcgctccc
     2521 ttcctggcgg cccgcacgat tcctgccggg caagagctga tgcgacaggg agatcccacg
     2581 ccggggatct tcctgatcct gtcgggtcgg gtcacggtgt tgctcgaagg gaccgacggc
     2641 cagcgggttc gtctccgcac tcttctcgga ggaacggtcc tcggcgagat cagcctgtat
     2701 cgagatgagc catgctcggc aaccgcaatc acggaaaccg aatgtgaggt cctccatctc
     2761 acgcccgagg cgttcaatga gttgtgccgg accgacccgg aagccgcggc caggttccac
     2821 gtcttcgtgg ctcgtaccct tgccggccgg gtgagctacg cgaatcgcgc catccgcgct
     2881 ctccacggtt gatcctcccg gccccgctgc actactactt caagcttcct tccgtgaccc
     2941 cggcgatgat ctggcggtgg aaaatcacgt agacggccac catcggaagc gtgatgatcg
     3001 tcagggcggc gaagagcagc tggtagtcgg tcagatagcg gctcgagaac gcgagcaagc
     3061 ccgtcgggat cgtcttcagg gcgtcgtcgg tgatatagag gagggcgagg aggaactcgt
     3121 tccaggcgct caacgcactg aagatggcca ccgtggccaa tccgggccgc agcaaaggca
     3181 gcaccagtgt ggtgaacatc ctgacgtcgc tgcagccgtc gacccgggct gcctcgaata
     3241 gctccttggg gagggagtcg agatagacct tgagcaaata gaccgccaga gagagtccca
     3301 tcgccgcata ggggatcatg agggcgagcc gggtgtcctt caggttcagg aactcgaaca
     3361 gtgagttctg ggcgatgaaa taggactgaa tcggcatcag gagcccgaag gcgaacaaac
     3421 ccagcagcag accgcggccg ccgaaccgaa acc
//
LOCUS       DASTXO010000424         2367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1277588, whole genome shotgun sequence.
ACCESSION   DASTXO010000424 DASTXO010000000
VERSION     DASTXO010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277588"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..1203)
                     /locus_tag="VFU96_08890"
     CDS             complement(151..1203)
                     /locus_tag="VFU96_08890"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5113421.1"
                     /translation="MTDHAPIRSQTSSGAIRRRAITRWTGPGWLWLLLLGIIVVASLI
                     AEPFLSSRNLTNVLRQMIPLGIVAVGQTLVILLAGVDLAVAATISMANTALMGIVAGQ
                     AANVPGAIGIVLGIGLLIGLVNGLIIVATRVPALIVTLGTASIVTGITFLYTDQSNFG
                     EPADVMNDIGFGSFGPVPMLFLLYVVVAAVGLIVQNKTAFGTHLFAVGGSEQVARASG
                     VRIARVHVAAYVACGFLAALAGVAMSMRMGAGEPLSGVGFDWDSIAAVVLGGTALTGG
                     RGGIGGTIGGVAIISILNNVMNLAGVSSFTQIVIKGIIVLIAVLLAAGVTRRVAEGVG
                     ARLQRRSTPVEGDVAA"
     gene            complement(1200..>2367)
                     /locus_tag="VFU96_08895"
     CDS             complement(1200..>2367)
                     /locus_tag="VFU96_08895"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEU5113422.1"
                     /translation="EVLPVVGADIDPATPVERLSIARRQMVEIAKALSLRAQMILLDE
                     PTSSLSIAEIELLHNVVRDLKQRGVSVVLVTHNLDEVFALADRVTVLKDGKVTLSVPV
                     PDVTAAEVIRAMIGRDLAHMFPPRTVDDRPRKTVIEVNGLTVPGRAEDISFELREAEV
                     LGFVGLIGAGRTDVAKALIGAIASRGGEVLIDGIDRKIKEPADAVANGIALVPEDRLA
                     DGLVMDLGVKQNISIPQLSRISRMGILDRDGESRLADQQIEALSIRTPSNSTVVHNLS
                     GGNQQKVVLGRWLAQGCRVLVLDEPTRGVDVGAKAEIYKTIRKLADGGTAVMLISSEL
                     PEILHLSDRIVVMSKGRIVAKLENNSVNQSDLASEEDLVKLALGVQSDAKAGES"
BASE COUNT          431 a          789 c          732 g          415 t
ORIGIN      
        1 tggtcgtgct caggaagtca ggcgagatga tggtcgagac gaccaccagc aacagcagga
       61 tggcccatgg gcccactatc gactggtgcc gacgaatggt tcccatccat cccctggcgc
      121 cggacggagc ctcggcttcg ctgaccgcgg tcacgctgcc acatctcctt ccacaggtgt
      181 cgaccgacgc tgcagccggg ctccaactcc ttcggcgacc cggcgtgtga ccccggccgc
      241 cagcaggacg gcgatcaaca cgatgatccc cttgatcacg atctgggtga acgaggacac
      301 gccggccaga ttcatcacgt tgttgaggat cgagatgatc gcgacgccgc cgattgtgcc
      361 cccgatccca ccccggccac cggttagagc ggtgccgccg aggaccacgg ctgcgatcga
      421 gtcccagtcg aaacccaccc ccgatagcgg ctcccctgcc cccattcgca tcgacatcgc
      481 gaccccggcc agggcggcga ggaatccaca ggcgacatac gcggcgacgt ggaccctggc
      541 gatgcggacc ccggaggcgc gggcgacctg ttcggacccg ccgactgcga agagatgggt
      601 gccgaatgcc gtcttgttct gcacgatgag tcccaccgcc gccaccacga cgtagaggag
      661 gaagagcatc ggaaccggtc cgaaggaccc gaaaccgatg tcgttcatca cgtcggccgg
      721 ctcgccgaag ttgctctggt ccgtgtagag aaaggtgatc ccggtgacga tgctggccgt
      781 ccccagggtg acgatcaggg cgggcacccg agtggcgacg atgatcagcc cgttcaccaa
      841 cccgatgagc aggccgatgc ccagcacgat tccgatcgcc ccaggcacgt tggcggcttg
      901 gccggccact atgcccatca gcgcggtgtt ggccatgctg atcgtggcgg cgaccgccaa
      961 gtcgacgccg gcgagcagga tcacaagtgt ttgcccgacc gcgacgatcc cgaggggaat
     1021 catctggcgg agcacgttgg tcaggtttcg cgaggagagg aatggctcgg cgatcaggga
     1081 tgccaccacg atgattccca gaagcaggag ccacagccac ccaggcccgg tccatcgagt
     1141 gatcgctctc cgccggatcg ccccactgct cgtttgcgac ctgatcggtg cgtggtctgt
     1201 catgactcgc ccgctttggc gtcgctctgg acgcccagag ccaacttcac caggtcttcc
     1261 tcgctggcca gatcggactg gttgaccgag ttgttctcca gcttggccac gatccggccc
     1321 ttggacatca ccacgatgcg atcgctcagg tggaggatct cgggaagctc agacgagatc
     1381 agcattaccg ccgttccccc gtcggccagt ttcctgatcg tcttgtagat ctccgctttg
     1441 gctccgacat cgaccccacg ggtgggctcg tcgagcacca ggacacggca accctgggcc
     1501 agccatcgtc ccagcaccac cttctgctgg ttccccccgc tgagattgtg cacgaccgtc
     1561 gagttcgatg gggttcggat gctcagcgcc tcgatctgtt gatcggcgag acggctctcc
     1621 ccgtcccggt cgaggatccc catgcggctg atacgactga gctgggggat gctgatgttc
     1681 tgctttaccc ccaggtccat cacgaggcca tcggccagcc gatcctcggg gacgagagcg
     1741 atgccattgg ccaccgcatc tgccggctct ttgatcttgc ggtcgatgcc atcgatgagc
     1801 acttcgccgc cacggctggc gatagcaccg atcagggcct tggccacatc ggtgcggcca
     1861 gccccgatca atccgacgaa tcccagaact tcggcctctc tcagctcgaa gctgatgtcc
     1921 tcggcgcgcc cgggaacggt cagcccgttg acctcgatca cggtctttct cggacggtcg
     1981 tccacggtcc gaggagggaa catgtgcgcc aggtcacggc cgatcatggc ccggatgact
     2041 tcggcagcag tgacgtccgg gactggaacc gacagggtga cctttccatc cttgagcacc
     2101 gtcacgcggt cggccaaggc gaaaacctca tcgaggttgt gcgtgacgag aacgacgctg
     2161 actccgcgct gtttcagatc ccgaaccacg ttgtggagga gctcgatctc agcaatcgaa
     2221 agtgaggacg tgggctcatc gaggaggatc atctgagccc ggaggctgag cgccttggcg
     2281 atctccacca tctgccgacg ggcgatggac aggcgttcta ccggggtggc cggatcgatg
     2341 tcagcgccga ccaccggcaa cacttcg
//
LOCUS       DASTXO010000425         5400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_430910, whole genome shotgun sequence.
ACCESSION   DASTXO010000425 DASTXO010000000
VERSION     DASTXO010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430910"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="VFU96_08900"
     CDS             complement(<1..904)
                     /locus_tag="VFU96_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005185915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5113423.1"
                     /translation="MSDISFERPDPRDFLDIDSLLSEEEVMLRDTVRTYVQKEIVPNV
                     GDWWDEGVLPQEALAKAFGDIGLLGMHLQGYGCAGTSATAYGLACLELEAGDSGIRSF
                     VSVQGSLVMFPIWKFGSEEQKQTWLPRMASGEIIGCFGLTEPDSGSDPASMRTNARRD
                     GSDWVINGTKMWITNGSIADLAVVWARTDEGVRGFLVPTDTPGFSAPEIKKKASLRAS
                     VTSELILDDVRVPDDFVLPGVSSMRGPLSCLNEARFGIAFGAMGAARACYESALDYSI
                     ERHQFDRPIASFQLTQRKLVEMMVA"
     gene            complement(946..1410)
                     /gene="purE"
                     /locus_tag="VFU96_08905"
     CDS             complement(946..1410)
                     /gene="purE"
                     /locus_tag="VFU96_08905"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEU5113424.1"
                     /translation="MSHKVAIVMGSGTDRPVMEKAAETLESFGISSEVRVLSAHKTPD
                     QALEFAAGASAQGFSVIIAGAGKAAHLAGVMAAKSLLPVIGVPINASLDGMDALLATV
                     QMPTGIPVATVAVDGATNAALLAVAILAIGDEGLTTKLATYRSDLAEKTLAG"
     gene            1476..2078
                     /locus_tag="VFU96_08910"
     CDS             1476..2078
                     /locus_tag="VFU96_08910"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEU5113425.1"
                     /translation="MFAMEPLQPSLFGESEPVPDARFASLERVDLDPESWLDYAPGFL
                     VGDARLYDVLTETVQWRQPEVRMYDRIVKTPRLVGNVAADSHPVIAEMIELLSERYRI
                     TMDQVSAGWYRTGIDSVAWHGDRIARDLPHSIVATVSLGGPRRFLIRPRGGGESMGFD
                     LGRGDLVVMGGACQRLWEHTIPKSASAPPRIALMFRHRYD"
     gene            complement(2069..3283)
                     /gene="purD"
                     /locus_tag="VFU96_08915"
     CDS             complement(2069..3283)
                     /gene="purD"
                     /locus_tag="VFU96_08915"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013349847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU5113426.1"
                     /translation="MRILLVGSGGREHAMGWKLASSPRVEDLISLPGNPGLARLGAVV
                     DGISATDVGAIAAMARVQAVDLVVVGPEAPLAAGVVDAVAALGIPVFGPTRAAARLES
                     SKTFAKEVMARAGVATASSHSFSEPEAAREHLSRRDGPLVVKADGLAAGKGVLVTDDR
                     DEAAAWAERWLPEGPVVVEDFLDGPEVSLFAICTDSGAVPLEPARDYKRLLDGDEGPN
                     TGGMGSFSPVADLPEGLAEETMRAVVEPTIRQMDEEGSPFRGFLYVGLVLTTEGPKVL
                     EFNVRLGDPETQAILPRMTTDLVDVLEGATPEWSRDAAVNVVLAARGYPEAPVKGAAV
                     HGLEAVPDDVLVFHAGTRSDGKRLLVDGGRVVDVVGLGSSVSEARAKAYEAVGAVSWP
                     GMQFRTDIAIQS"
     gene            complement(3280..4554)
                     /locus_tag="VFU96_08920"
     CDS             complement(3280..4554)
                     /locus_tag="VFU96_08920"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEU5113427.1"
                     /translation="MPATVVLGAQWGDEGKGRVVDFFAESADFVVRYQGGNNAGHTIV
                     VGEQRLALSMIPSGILYPDCIPVIASGTVIDPRVLIAEMDMAAEKNLDPSRLRVSSNA
                     HVIMPYHRKLDAAIERYLGKNQIGTTKKGIGPAYTDKYARFGIRVQDLFDPKIFREKV
                     EAALVEKNKVLPRVYNTLPMDPEAIIGEYAGYAERLEPHVTDTSLLLWEGLQAGKQVL
                     FEGAQGTLLDLDHGTYPFVTSSNPTAGGASVGSGIGPRAIDEIIGVAKAYISRVGTGP
                     FPTELHDEVGDRMIELGGEYGTVTGRRRRCGWLDLVALKYACRVNTFTSLFLTKLDIL
                     SAFDTIKVATAYTTQSGRHEHFPAQQSVLYHCEPEYDELPGWQDDITGVRSYSDLPST
                     ARAYIEYIQEAAGVPVGWVSVGPERSQLIEIS"
     gene            4635..>5400
                     /locus_tag="VFU96_08925"
     CDS             4635..>5400
                     /locus_tag="VFU96_08925"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU5113428.1"
                     /translation="MSTTSTRVYRGGKLADEDFPVADVSEYLAEPDTIVWVDLIGPST
                     KELLDLAEELGLHELAVEDAVGQGQRPKVDRYPTHLFLTTYHVAVDLDAARLDETEMN
                     AFFNDRWLITVRKTDGLPMERVRARWDRSPGLMRFGVGFLLYGLLDVIVDDYFTVLAE
                     FDEFYEGVSEAIFAERPLEPSRQRHWFEMRRATVRFHRLVVPMRETVSALMRHEQEIV
                     PEGLYPYFQDVYDHILRISESSDSLRDLVTAIVETNF"
BASE COUNT          952 a         1767 c         1709 g          972 t
ORIGIN      
        1 ccgccaccat catctcgacg agcttcctct gggtcagctg aaaagaggcg atcggccggt
       61 cgaactggtg cctctcgatc gagtaatcca gagccgattc gtaacaggcg cgtgccgccc
      121 ccatcgcacc aaatgcgata ccgaacctcg cctcgttgag acatgacagc ggcccacgca
      181 tcgacgacac acccgggagt acgaaatcgt cgggaactct gacgtcgtcg aggatcaatt
      241 ccgaggtcac agaagcacga agcgacgcct tcttcttgat ctccggcgcc gagaaccccg
      301 gtgtgtcggt gggaacgagg aatccccgaa ccccttcgtc ggtgcgggcc caaacgactg
      361 ccaggtcggc gatcgacccg ttggtgatcc acatcttggt tccgttgatc acccagtcgg
      421 acccgtcccg gcgagcattg gtcctcatcg atgccgggtc ggagcctgag tcgggctcag
      481 tgaggccgaa gcagccgatg atctcgccgc tcgccatccg ggggagccaa gtctgcttct
      541 gctcctcgga gccgaacttc cagatgggga acatcacaag cgagccctgg acggagacga
      601 atgaccgaat cccggagtcc cccgcctcca actcgagaca ggcgagcccg taggcggtgg
      661 ccgacgttcc ggcacatcca tagccctgaa gatgcatccc gagcaggccg atgtcgccga
      721 acgccttggc cagggcctcc tgggggagga caccctcgtc ccaccagtcg ccgacattgg
      781 gcacgatctc cttctgcaca taggtcctga ccgtgtcccg caacatgacc tcttcctcgg
      841 agaggaggga gtcgatgtcg aggaagtccc tcgggtctgg tcgttcgaat gagatgtcac
      901 tcatgcaggg ccgatggtat cggagtcggt attggcagac ctcagtcagc cggcgagggt
      961 cttctcggcc aggtcggatc gataggtggc cagcttggtg gtgagccctt cgtcgccgat
     1021 ggcgaggatg gccacggcga ggagtgcggc gttggtcgcc ccgtccacag cgaccgtggc
     1081 caccgggatc cccgtcggca tctgaaccgt ggcgagcagc gcatccatgc cgtcgaggga
     1141 ggcattgatg gggactccga tcaccggcag cagcgacttg gccgccatca cccctgcgag
     1201 atgcgccgcc ttccccgcgc cggcgatgat caccgagaaa ccctgcgcag aggccccggc
     1261 ggcaaactcg agtgcctggt cgggggtctt gtgggcggag agaaccctca cctcgctgga
     1321 gatcccgaac gactccaagg tctcagcggc cttctccatg accggccggt cggtccccga
     1381 tcccatgacg attgcgactt tgtgactcat gagagggcga cggtaaccga ctccctgctg
     1441 atcactttcg ccacaagacc actatcatcg aacacatgtt cgctatggaa cctctgcagc
     1501 cgtctctgtt cggtgagagc gagccggttc cggatgccag gttcgccagt ctggagcggg
     1561 tcgatctcga tcccgagtcg tggctcgact acgcgccggg tttcctcgtg ggcgacgccc
     1621 ggttgtacga cgtcctgacc gagacagtgc aatggcgcca acccgaagtc cgcatgtacg
     1681 accgaatcgt caagactccc cgcctggtcg ggaacgtcgc agccgactct catcccgtca
     1741 tcgccgagat gatcgagctg ctctcggagc gctaccggat caccatggat caggtcagtg
     1801 ccggctggta ccgaaccggg atcgactccg tggcctggca cggtgatcgc atcgcccgcg
     1861 acctgcccca ttccatcgtc gccactgtct ctttgggtgg tccgcggaga ttcctgatcc
     1921 ggcccagggg tggtggggaa tcaatgggat tcgacctcgg tcggggagat ctcgtggtga
     1981 tgggtggcgc ctgtcagcgg ctgtgggagc acacgattcc caaaagcgcc tccgccccac
     2041 cacgaatcgc cctgatgttt cgccaccgct acgactgaat ggcgatatcg gtccggaact
     2101 gcatgcctgg ccagctcacc gcaccgaccg cctcgtacgc cttcgctcgg gcttcgctca
     2161 ccgaggagcc gagcccgacc acatcgacta cacggccccc gtctaccagc agccttttgc
     2221 cgtctgacct ggtcccggcg tggaacacca gcacatcgtc gggcaccgct tccaggccat
     2281 ggacggcggc tcctttgacc ggtgcctccg ggtatccccg tgcggccagc acgacattca
     2341 ctgccgcatc tcgggaccat tcgggcgtgg cgccctcgag gacgtcgacg agatcggtcg
     2401 tcatgcgggg caggattgcc tgagtctcgg ggtcaccgag tcgaacgttg aactcgagca
     2461 ccttggggcc ttcagtggtg agcaccaggc ccacgtacag gaaaccccgg aacgggctcc
     2521 cttcctcatc catctgtctg atggtcggct cgacgacggc gcgcatggtc tcctcggcaa
     2581 ggccctcggg caggtcggcg actggcgaga acgaacccat cccaccggtg ttcggacctt
     2641 cgtctccgtc gagcagacgt ttgtagtcac gggcaggctc gagcgggaca gcgccggagt
     2701 cggtgcaaat ggcgaacagg ctcacttccg gcccgtcgag gaaatcctcg accaccaccg
     2761 ggccctcggg gagccagcgt tctgcccacg ccgccgcctc gtctcggtca tcggtgacca
     2821 ggacaccctt gccggcggcg aggccatcgg ccttgaccac cagaggtccg tcacgacggg
     2881 agagatgctc gcgggcggct tcgggctcgg agaacgaatg cgatgatgcg gtcgccaccc
     2941 cggccctcgc catcacctcc ttggcgaacg tcttcgatga ctccaatcgt gcggccgccc
     3001 gggtcggccc gaagacgggg atgccgaggg cggccaccgc atcgacgacg ccggcggcga
     3061 ggggagcctc cggcccgact acgaccaggt ccaccgcctg gacccgggcc atcgccgcga
     3121 tcgccccgac atcggtggcc gagatcccgt ccactacggc tccgagccgg gccagacctg
     3181 ggttgcccgg cagagagatc agatcctcca cccggggcga cgacgccagt ttccagccca
     3241 tggcgtgctc acgtcccccg ctgcccacca ggaggatcct catgagatct ctatgagctg
     3301 gctgcgctca gggcctaccg acacccatcc cacgggtacc ccggccgcct cctggatgta
     3361 ctcgatgtag gcgcgggcgg tggatgggag atcggaatag gagcgcacgc ccgtgatgtc
     3421 atcctgccat ccggggagct cgtcgtactc cggctcgcag tgatagagga cgctctgctg
     3481 agccggaaag tgctcatgac gcccgctctg agtcgtgtac gcggtcgcca ccttgatggt
     3541 gtcgaaggcg gagaggatgt ccagcttggt gaggaagaga ctggtgaagg tgttgacccg
     3601 acaagcgtat ttcaaggcga ccagatccag ccagccgcat ctcctcctgc ggccggtgac
     3661 ggtgccgtat tcgccaccga gctcgatcat ccggtccccg acttcgtcgt gcagctcggt
     3721 gggaaacggt ccggtcccga ctcgtgagat gtaggccttg gccacgccga tgatctcgtc
     3781 gatcgccctg gggccgattc ccgagccgac tgacgcccct ccggccgtgg ggttggaaga
     3841 agtgacgaag ggatacgtgc cgtggtcgag gtcgagcaaa gtgccctggg ccccctcgaa
     3901 caggacctgc ttgccagcct ggagaccttc ccagaggagc agggaggtgt cggtgacatg
     3961 gggctcgagc cgctcggcgt agccggcgta ctccccgatg atcgcctcgg gatccatcgg
     4021 cagtgtgttg tagacgcggg gcaggacctt gttcttctcc acgagggcag cctctacctt
     4081 ctcgcggaag atcttggggt cgaagagatc ctggacccgg atcccgaatc gggcgtactt
     4141 gtcggtgtag gccggaccga tgcccttctt cgtcgtaccg atttggttct tgcccagata
     4201 gcgctcgatg gcagcgtcga gcttgcggtg atagggcatg atcacgtgag cattcgagga
     4261 gaccctcaat cgcgacgggt cgaggttttt ctctgcggcc atgtccatct cggcaatgag
     4321 cactctcgga tcgatcactg tccccgaagc gatgacaggt atgcagtcgg gatagaggat
     4381 cccggagggg atcatcgaca gggcgagacg ttgctcgccc acgacgatgg tgtgcccggc
     4441 gttgtttccc ccctgatagc ggacgacgaa gtcggctgac tcggcgaaga agtccacgac
     4501 ccgccccttc ccctcgtcac cccactgcgc gcccaagacg acggtcgctg gcatgacgtg
     4561 gagcgacggt agtcagcacg aggcgtcggt tggcgatcag gcggcgtgga gacctttagt
     4621 ctgtcgccgg gatcatgagc acgacatcga ccagggtgta tcggggcggg aaattggcgg
     4681 acgaggactt cccggtcgcc gatgtgtccg agtatctcgc cgagcccgac accatcgtct
     4741 gggtcgacct gatcgggcca tcgaccaagg agctgcttga cctggccgag gagctcggtc
     4801 tccacgaatt ggccgtggaa gacgcggtgg gtcagggcca gcgcccaaag gtcgaccgat
     4861 atccaaccca tctcttcttg accacgtacc acgtggccgt ggatctggat gcagcccggc
     4921 tcgatgagac cgagatgaac gccttcttca acgaccgctg gctcataacg gttcgcaaga
     4981 ccgacgggct cccgatggag cgggtccgag cccggtggga ccggtcaccg ggtctgatgc
     5041 gatttggcgt cggcttcctc ctctacgggt tgctcgacgt catcgtcgac gactatttca
     5101 cggtcctagc cgagttcgac gagttctacg agggggtgag cgaggcgatc ttcgccgagc
     5161 gcccgctgga gccgtcccgc cagcgccact ggttcgagat gcgtcgagcg accgtgcgct
     5221 tccaccgttt ggtggtcccg atgcgggaga cggtgagcgc attgatgcgt catgagcaag
     5281 agatcgttcc cgaggggctc tacccctact tccaggacgt ctacgaccac attctccgaa
     5341 tcagcgagtc ttccgactcc ctgcgcgacc tcgtcaccgc gatcgtcgag accaacttca
//
LOCUS       DASTXO010000426         2897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_762354, whole genome shotgun sequence.
ACCESSION   DASTXO010000426 DASTXO010000000
VERSION     DASTXO010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762354"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /gene="pstA"
                     /locus_tag="VFU96_08930"
     CDS             <1..753
                     /gene="pstA"
                     /locus_tag="VFU96_08930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU5113429.1"
                     /translation="LSPAAESAGIWQAIMGSLALTGFVIAVAFPFGVAAAVYLEEYAP
                     DNRVTRFINANIRNLAGVPSIVYGILGLFLFVEILMRGVFGASEGIAGRNVVAGGLTL
                     AVLVLPIMIITTSEALRAVPGGLREASLAVGATRWETIRHHVLPVAVPAILTGSVLTI
                     ARAFGESAPLLLAGAQLSLFFSVSSDASFADLMLTQPYTALPIVVFNFARQPQAEFVS
                     LTAASIVVLLVMLLTVNAAAVIARDRYERRYR"
     gene            774..1661
                     /gene="pstB"
                     /locus_tag="VFU96_08935"
     CDS             774..1661
                     /gene="pstB"
                     /locus_tag="VFU96_08935"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018723784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEU5113430.1"
                     /translation="MTTQAETSRKQELPIEVTVADHTTGQDAASTVDAAEERTPIFEL
                     GGLTVFYGSFRAVRDVDMTIRQNRITALIGPSGCGKSTVLRCFNRMNDLIDSARVEGR
                     VEYHGVDLYGEKVDPVEVRRRIGMVFQKPNPFPKSIYENIVYGPRIAGFKGNYDDLVE
                     ETLIKAALWDEVKDKLKESGMALSGGQQQRLCIARAIATSPDVVLMDEPCSALDPIAT
                     LKIEELMLDLKKDYTIVIVTHNMQQAARVSDQTAFFSVNVDEEGQRTGYLVEFSQTEQ
                     IFTAPVQKQTEDYITGRFG"
     gene            1658..2365
                     /gene="phoU"
                     /locus_tag="VFU96_08940"
     CDS             1658..2365
                     /gene="phoU"
                     /locus_tag="VFU96_08940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU5113431.1"
                     /translation="MTSAPDTNLHFHQELEALEHTVLGMVDRAEHMVEMAVDSVTTGN
                     QELADRVIAIDADIDKTYFEVHQMWTTLMARFQPLGSDLRKMSVLLQLNATFERMGDQ
                     CVNIARVTQQNEGLPRVERICDQIQEMGDLVRPMIRTSIEAYLRQDLDEARLLPAMDQ
                     PVDRLNANMFKEIVAVAADPQMLEWATKMMMASRALERVGDQAVDFAEQTAYLITGKR
                     AEFVESGLDKSVDDPDG"
     gene            2349..>2897
                     /locus_tag="VFU96_08945"
     CDS             2349..>2897
                     /locus_tag="VFU96_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015796763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5113432.1"
                     /translation="MTRTDERRRHRVLVVEDEPGLAESVRYALDVEGFEVLVADSGVV
                     GLESARVNQPDLVLLDLMLPGMSGLDVCRQIRMSSDVPIIMLTAKDAESDKVAGLELG
                     ADDYMTKPFSMRELVARIRAQLRRAAKSGVLAESNEVLRGGPIELDIDAHQTRLEGEP
                     VDLRPKEFELLESLMRRRNRLAA"
BASE COUNT          550 a          902 c          911 g          534 t
ORIGIN      
        1 ctttcgcccg cggccgagtc ggccgggatc tggcaggcga taatgggatc gctggccctg
       61 accgggttcg tcatcgcggt cgcgttcccg ttcggagtgg cggcagccgt ctatctcgag
      121 gaatacgcgc ccgataatcg ggtgaccagg ttcatcaacg ccaacattcg caatctggcc
      181 ggggtgccgt cgatcgtcta cgggatcctc ggtctcttct tgtttgtcga gatcctgatg
      241 cggggtgtct tcggagcctc ggaaggaatc gcgggccgaa atgtcgtggc gggtggcctc
      301 accctcgcgg tcctggtgct cccgataatg atcatcacca cctcggaagc cctgcgggcg
      361 gtccccggcg gtttgcgaga ggcttcgttg gcggtcgggg cgacccggtg ggagacgatc
      421 aggcatcatg tcctcccggt cgccgtcccc gccattctca ccggctcggt cctcaccatc
      481 gccagagcct tcggcgaatc ggcgcccctc ttgttggcag gggctcaatt gtcactcttc
      541 ttcagcgtct ccagcgacgc ctcgttcgcc gatctgatgt tgacccagcc ctacaccgcc
      601 ctccctatcg tcgtcttcaa cttcgcccgg cagccccagg ccgagttcgt gtcgctcacc
      661 gcggcgtcga tcgtcgtcct tctggtgatg ttgttgaccg ttaacgccgc ggccgtcatc
      721 gctcgtgacc gctacgagcg gagatatcga tgatcgacca ggaaggcgag ccgatgacca
      781 cacaagccga gacgagccgc aagcaggagc tgccgatcga ggtgaccgtg gccgatcaca
      841 ccaccggtca ggatgccgcg tccacggtgg atgctgccga ggaacgcacc ccgatcttcg
      901 agctcggggg cctgacagtc ttctacggat cgttccgggc cgtgcgcgac gtcgacatga
      961 cgatccgcca gaaccgcatc accgctctca tcgggccctc cggctgcggt aagtccaccg
     1021 tcctccgttg tttcaaccgg atgaacgacc tcatcgattc cgctcgggtc gagggccggg
     1081 tcgagtacca cggggtagac ctctacgggg agaaggtgga tccggtcgag gtgcggcgcc
     1141 ggatcggcat ggtctttcag aaacccaacc cattcccgaa gagcatctat gagaacatcg
     1201 tgtacgggcc gcggatcgcc ggcttcaagg gcaattacga cgacctggtg gaagagaccc
     1261 tgatcaaggc cgccctgtgg gacgaggtca aggacaagct caaggaatcg gggatggcgc
     1321 tgtcgggtgg tcaacaacag cgtctttgca tcgcacgtgc catcgccacg agccccgacg
     1381 tcgtgctgat ggacgaaccc tgctcagccc tcgatcccat cgccaccttg aagatcgagg
     1441 agctgatgct cgatctgaag aaggactaca cgatcgtgat cgtcacccac aacatgcagc
     1501 aggcagcccg ggtttcggat cagacggcct tcttctcggt aaacgtcgac gaggaggggc
     1561 agcgaaccgg ctacctcgta gaattcagcc agaccgagca gatcttcacc gctcctgtcc
     1621 aaaagcagac cgaggactac atcacgggac gattcggatg accagtgcgc cagacaccaa
     1681 ccttcatttt caccaagagc tggaggccct ggagcacacc gtcctcggca tggtcgatcg
     1741 ggccgagcac atggtcgaga tggcggtgga ctcggtcacc accggcaacc aggagctggc
     1801 cgatcgggtg atcgccatcg acgcagacat cgacaagacc tatttcgagg tccaccagat
     1861 gtggacgacc ctgatggctc gttttcagcc actcggctcc gacctgagga agatgagcgt
     1921 cctcctccag ctcaacgcca cattcgagcg catgggcgat cagtgcgtga acatcgcccg
     1981 cgtcactcaa caaaacgagg gtctgccccg ggtggagcgc atctgcgatc agatccagga
     2041 gatgggcgac ctggtgcgcc cgatgatcag gacctcgatc gaggcctacc tgcgtcagga
     2101 cctggacgaa gcgcgtcttc tgcccgctat ggaccagccg gtcgaccgtc tcaacgccaa
     2161 catgttcaaa gagatcgtgg cggtcgccgc ggacccgcag atgctggagt gggcgacgaa
     2221 gatgatgatg gcgtcccggg ccctggagcg ggtcggggac caggcggtgg acttcgccga
     2281 acaaacggca tacctgatca caggcaaacg agccgagttc gtcgaatcgg gcttggacaa
     2341 gtcggtcgat gacccggacg gatgagcgtc gaaggcatcg cgtccttgtc gtagaagacg
     2401 agccggggct tgccgagtcg gttcgatatg ccctcgatgt ggagggattc gaggtgctcg
     2461 tggccgattc cggggtcgtc gggcttgagt cggcgcgggt caaccagcct gacctcgtgc
     2521 ttctcgacct catgctcccc ggcatgtccg ggctggatgt atgccgacag atccggatgt
     2581 cttcggatgt cccgatcatc atgctcaccg ccaaggacgc cgaatcggac aaggtcgccg
     2641 gcctcgagct cggcgccgac gactacatga ccaagccgtt ctcgatgcga gaactggtgg
     2701 cacggatccg ggcacagtta cggagggcag caaagagtgg ggtgctcgcc gagtcgaacg
     2761 aggtgttgcg cggcggcccg atagagctcg atatcgatgc ccatcagacc agactcgaag
     2821 gcgagccggt cgacttgcgt cccaaggaat tcgagctcct cgagtcgttg atgcgtcgga
     2881 ggaatcgact ggctgct
//
LOCUS       DASTXO010000427         3714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_504501, whole genome shotgun sequence.
ACCESSION   DASTXO010000427 DASTXO010000000
VERSION     DASTXO010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504501"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="VFU96_08950"
     CDS             <1..686
                     /locus_tag="VFU96_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU5113433.1"
                     /translation="LIGPNGSGKSTLLGAVAGLIDPVGGSIDVPPEDGLAQRISYVLQ
                     STKVNDTLPVTVREVVTMGRYAGSSRRLDGVDHAAVDEAMGRMGITHLAGNHLRELSG
                     GQRQRVLVAQGLAQEHEILLLDEPLTGLDLTSAQAIDQVIHDETQTGCTIVMTTHDLA
                     EAEVAAHVVLLSGRVVASGTAEEVLTLENLTAAYGPNLLHVEGERLFIDDPAHRPVPG
                     RHDHRDRAV"
     gene            complement(770..1030)
                     /locus_tag="VFU96_08955"
     CDS             complement(770..1030)
                     /locus_tag="VFU96_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEU5113434.1"
                     /translation="MNIYVGNLPFSTTAADLETLFGEYGAVDNAAIINDRETGRSRGF
                     GFVEMANDAGAKAIEELDGADYNGRNLKVNEARPREERGPRR"
     gene            complement(1201..1710)
                     /locus_tag="VFU96_08960"
     CDS             complement(1201..1710)
                     /locus_tag="VFU96_08960"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU5113435.1"
                     /translation="MAKQYPSPPDLTIDLDKGYTATLDTNHGEIVIELDPARSPLTVN
                     NFVFLARDGYYDGVVFHRVIENFMIQGGDPTGTGTGGPGYKFRDEIEGQGSYGRGTVA
                     MANAGPNTNGSQFFICHTDVGLPHSYTIFGKVTSGMDAVDSIATTSTDRGDRPQSEVV
                     INKVTITED"
     gene            complement(1758..2342)
                     /locus_tag="VFU96_08965"
     CDS             complement(1758..2342)
                     /locus_tag="VFU96_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011431553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU5113436.1"
                     /translation="MAVTSTMLALGTKAPDFTLPDVVSGREVSLDDFDGSKGMLVMFI
                     CNHCPYVRHVRDELARFGRDYQDEDLGIVAISANDADQYPDDGPDAMRKEALRTGYTF
                     PYLFDAEQTVASAYTAMCTPDFFLFDADRDLVYRGRLDDSRPNSGTPVTGADLRAAVD
                     AVLSGQLVSTDQRPSMGCSIKWKPGNVPAYFGAA"
     gene            complement(2352..>3714)
                     /locus_tag="VFU96_08970"
     CDS             complement(2352..>3714)
                     /locus_tag="VFU96_08970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEU5113437.1"
                     /translation="IVGSGEDDQQAGTAAFSILEQIVEERMRRRMTTVIDTLGFDRDK
                     RRRWVALAHEADLPAFAVVFDTPPAQARVRNAEQVRPIPKTVLDKQLARFRVVRTEID
                     DDGFDSVLVEQPVALVAPSMVAKQHDAAEERVARPGHRFGLVVNRFDWGEDRSGMAMR
                     LASIASRAETAGFDDLWVMDHFRQIPQVGRQWEDIPEAYTTLAFLAGVTSGIRLGTLV
                     TGIGHRHPVVLGKMVATLDVLSGGRVTLGLGIGWDRKEHEGYGIPFQPVPARFEQLED
                     TLRMLPLLWGKGSPSFAGATFSAPELICYPRPIQDPIPILIGGSGERRTLRLVARYGD
                     ACNLLGRPDVVRRKVEVLHDHCAEIERDPAEVEVTHLVDMLAAPDRKALRDRVEELRG
                     RNMSAEAFMARYNAGVVEDQLDHVAGYRAAGATHSMVAMPDVHLEGSIEAFGEVIGAM
                     RRP"
BASE COUNT          654 a         1166 c         1213 g          681 t
ORIGIN      
        1 tgctgatcgg acccaacgga tcaggcaagt cgaccctcct aggtgccgtg gccggcctca
       61 tcgatcctgt gggcgggtca atcgatgtgc cgccagaaga cgggttggcg cagaggatct
      121 cctacgtttt gcagagcacc aaggtcaacg acactttgcc ggtgaccgtg agagaagtgg
      181 tgacgatggg gcggtatgcc gggtccagtc gccgtctcga tggcgtggac cacgccgcag
      241 tggacgaggc gatgggaagg atggggatca cccacctggc cggcaaccac cttcgcgagc
      301 tatcgggtgg gcaacgacaa cgagttctgg ttgctcaggg cctggcccag gagcacgaga
      361 tcctgctcct cgacgagccg ctgaccggcc tcgacctgac ctctgcacag gccatcgacc
      421 aggtcatcca cgacgagacc caaaccgggt gcacgatcgt gatgacgact cacgacctcg
      481 ccgaggccga ggtcgccgct catgtggttc tcctctctgg ccgtgtggtc gcttcgggca
      541 cggcagagga ggtcctgacc ctcgagaacc tgacggccgc ctacggcccc aatctcctcc
      601 atgtcgaggg agaaaggctc ttcatcgacg atccggcaca ccgccccgtg ccgggaaggc
      661 acgatcaccg ggatcgggcg gtctgagtcg gctaccgacg ctgttgatcg gcgtgggtca
      721 ccgacggtga atatgggcgt cggtcaccga cggtgaatat gggcgtcggt caccgacgcg
      781 ggccgcgctc ctcgcggggc cgagcctcgt tcaccttgag gttgcgcccg ttgtaatcgg
      841 ccccgtcgag ctcttcgatt gccttggcgc cggcgtcgtt cgccatctcg acgaacccga
      901 acccgcgtga gcggccggtc tcgcgatcgt tgatgatcgc ggcattgtcc accgctccgt
      961 actcaccgaa cagagtctcg agatcggcgg cagtggtgct gaagggaagg ttcccaacgt
     1021 agatgttcat gacgtcccgt catcctttct aatgaagggc ctcttggcag cccttcgcca
     1081 tgtgtccggt acgaccctgc cctcgatccg gtcgcaatgc tggacgggaa gccaacgagt
     1141 cgccgccgaa gcagcgggcg gaacgctagt ggaatctgag cgattgtgtg ccgaaatcgg
     1201 tcaatcctcc gtgatcgtca ccttgttgat gaccacttcc gactgaggcc tgtctccccg
     1261 gtcagtcgag gtggtggcga tggagtccac ggcatccatg cccgacgtca ccttgccgaa
     1321 aatcgtgtag gagtgaggaa gtccgacgtc ggtgtggcag atgaagaact gggagccatt
     1381 ggtgttgggc ccggcgttgg ccatagccac cgtaccccgg ccatagctgc cctgaccctc
     1441 gatctcatcg cggaacttgt aaccggggcc accggtcccg gtgccggtcg ggtcaccgcc
     1501 ctggatcatg aagttctcga tgacacgatg gaaaacgacg ccgtcgtagt agccgtcacg
     1561 ggcgaggaag acgaagttgt tgacggtcag cggtgagcgg gccgggtcga gctcgatgac
     1621 gatctcgcca tgattggtgt ccagggtggc ggtgtatccc ttgtccagat cgatggtgag
     1681 gtctgggggt gatggatatt gcttggccac tcagggcctc cttgtgtcgt gaaacgtaga
     1741 aacaagacca gacgctgtca ggcagccccg aagtaggcgg ggacgttgcc cggcttccac
     1801 ttgatcgagc aacccatcga gggccgctga tcggtgctca ccagctgccc ggagagaacg
     1861 gcgtccaccg cggcgcggag atcggcgccg gtgaccgggg tcccgctgtt tggccgcgaa
     1921 tcgtccaacc ggccccggta gaccaggtcc cgatccgcgt cgaacaagaa gaaatccggg
     1981 gtgcacatgg cggtgtaggc ggaggccacg gtctgttcgg cgtcgaagag gtaggggaag
     2041 gtgtagccgg tgcggagggc ttccttcctc atcgcatcgg ggccgtcgtc cgggtattgg
     2101 tcggcatcgt tcgccgatat cgccacgatc ccgaggtcct cgtcctggta gtccctcccg
     2161 aaacgagcca gttcgtctcg cacgtggcga acgtaagggc agtgattgca gatgaacatg
     2221 acgagcatgc ccttcgaccc gtcgaagtca tcgagcgaga cctctcgtcc cgagacgacg
     2281 tcgggcaggg tgaaatcggg ggctttggtg ccgagcgcga gcatcgttga ggtgacagcc
     2341 atgtcgccag tctaagggcg cctcatggct ccgatcacct ccccgaatgc ctcgatgctt
     2401 ccttcgaggt gcacgtcggg catcgccacc atcgagtggg tcgccccggc cgcccggtag
     2461 ccggcgacgt gatcgagttg gtcctcgacg acgccggcgt tgtaacgggc catgaacgcc
     2521 tcggctgaca tgttccgtcc ccgcagctct tccacacggt cccgcaatgc tttccgatca
     2581 ggcgcggcca gcatgtccac cagatgggtc acctcgacct ctgcggggtc acgctcgatt
     2641 tccgcgcaat gatcgtggag cacctcgacc ttgcgacgga ccacgtcggg gcgtcccagg
     2701 agattgcagg catcgccata tcgggcgacc agtcggagcg ttcgtcgttc gcccgatccg
     2761 ccgatgagga tggggatcgg gtcctggatc ggtcgcggat agcagatcaa ctccggagcc
     2821 gagaacgttg ccccggcgaa cgacggtgag cccttgcccc agagcagggg gagcatgcga
     2881 agggtgtcct cgagctgttc gaatcgagcc ggcaccggtt ggaaggggat cccgtatccc
     2941 tcgtgctctt ttcggtccca tccgattccg agaccgaggg tcaccctccc gccggagaga
     3001 acgtcgagcg tggccaccat cttgccgagg accaccggat gccgatgccc gatcccggtc
     3061 accagtgtcc ctagccggat accactggtc acaccagcaa ggaaggcgag cgtggtgtag
     3121 gcctcgggaa tgtcttccca ttggcggcct acctgaggaa tctgccggaa atggtccatg
     3181 acccataggt cgtcgaatcc cgcagtctcg gcccgggagg cgatcgaggc gagacgcatt
     3241 gccatcccgg agcggtcctc gccccagtcg aaccggttga ccaccaggcc gaatcggtgc
     3301 cccggtcggg ccacgcgctc ctcggcggcg tcatgctgct ttgcgaccat cgagggagca
     3361 accagagcca ccggctgttc gaccagcacg ctgtcgaagc cgtcgtcgtc gatctccgtc
     3421 cgaactaccc gaaacctggc gagttgcttg tcgaggacgg tcttcgggat gggccgaacc
     3481 tgttctgcat tccgcacccg agcctgcgcg gggggtgtgt cgaacacgac cgcaaaggct
     3541 gggagatcgg cctcgtgggc tagggcgacc catcgccggc gcttgtcccg gtcgaagcca
     3601 agggtgtcga tcaccgtggt catgcggcgt cgcattcgct cctcgacgat ctgctccagg
     3661 atcgagaaag cagcggtgcc cgcctgctgg tcatcctcgc ctgagcccac gatt
//
LOCUS       DASTXO010000428         3169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_615147, whole genome shotgun sequence.
ACCESSION   DASTXO010000428 DASTXO010000000
VERSION     DASTXO010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615147"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            444..1124
                     /locus_tag="VFU96_08975"
     CDS             444..1124
                     /locus_tag="VFU96_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113438.1"
                     /translation="MPTSDPRESEGPRLTARFWRSRRAAALAGIVFAVLLITAMTMLR
                     IALSEGSLQLLQSDAPRRTLIRISLNLVPFAGIAFLWFVGVVREQIGVVEDRLFSTVF
                     LGSGLLFLAMVFVGAVTSTSLLEMLAVGGVNAEIWAFGRSSTQGLISVYAMRMAAVFT
                     LSVSTLGLRTSAMPRWVSFIGYAVALVLLVAAGEHRWIQLVFPTWVLLVSIVILFTRP
                     PVRTSVTN"
     gene            1967..>3169
                     /locus_tag="VFU96_08980"
     CDS             1967..>3169
                     /locus_tag="VFU96_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016878486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU5113439.1"
                     /translation="MTAPEVSRSVLPIPQRPHVGLTTYDAKDPDTAFPPITPLRPPEG
                     APNVLIVLIDDAGFGSSSAFGGPCNTPTFERLAGGGLRFNRFHTTALCSPSRQALLTG
                     RNHHAVGMGGITEIATSAPGYNSIRPASAAPLAETLKLNGYSTAAFGKCHEVPVWETS
                     PMGPFNQWPLGSGFEYFYGFVGAETNQWYPALYDGVTPIEPPKTPEEGYHLTEDLADK
                     AITWVSQQKSLMPDKPFFVYFAPGATHAPHHVAQEWIERYRGRFDQGWDAIREETFAR
                     QKQLGVIPPETELSARPAQIEAWENVPDDLKPVLARQMEVYAGFLEHTDHQVGRLIDN
                     LEDLGILDDTLIYVMIGDNGASAEGTTTGTFNEIISLNGAADLETTEFMVAHIDGFGS
                     PAAYNHYAV"
BASE COUNT          567 a         1005 c         1012 g          585 t
ORIGIN      
        1 agatcaggtg aacctggatc gatcgacgct tttcctgtcg ggtacccgga tgaggagatc
       61 ttcacgcgat ctgtgtgacg acagccgccg ctgtgtggcg gacgatcatc ctgtcgctga
      121 gtctccacat cggcgacacc cgtcgaggag ccaggatctg atgaccagcg agatgtcgac
      181 gatccgcgct gggtcatcca gcccggtagc cggggtctag gtgacctatg ccgtcgacca
      241 cgcccatgag ggatatcgcg gggagacacg gatggagtat cgatgatgtg cactagcgcc
      301 tccttcgtgg ctcagatgcg cgaagtcgag gacctatacc ggtcccggtc ccgagagatg
      361 gccctggagt ggaagaggca acccctgttc aaaccatggc tcgataacgt catgcgcctc
      421 acctccgccg tcccatgagc agcatgccga cctccgatcc gcgcgagtca gaaggcccga
      481 ggctgaccgc ccgtttttgg cgcagccggc gcgccgcagc ccttgccggc atcgtcttcg
      541 cggtcttgct gatcacggcg atgacgatgc tccggattgc gttgagcgag gggagtctgc
      601 aactgttgca gtccgatgct ccgcggcgga cgctgatccg gatcagcctc aaccttgtcc
      661 cgtttgcggg catcgcattc ttgtggttcg taggcgtcgt ccgcgagcag atcggcgtgg
      721 tcgaggaccg gctgttctcg accgttttcc tgggcagcgg acttctcttc ttggcaatgg
      781 tgttcgtcgg cgccgtcaca tcgacgagcc tcttggagat gttggcggtg ggcggtgtaa
      841 acgccgaaat ctgggcgttc ggtcgcagtt cgacgcaggg actcatttcc gtatacgcga
      901 tgcggatggc ggccgtgttc accctgtcgg tgagcaccct tggcctacgc acctcggcca
      961 tgccccgttg ggtctccttc atcgggtacg ccgtcgccct tgtgctcctg gtggcagccg
     1021 gagagcacag gtggatccag ctcgtcttcc ccacctgggt gctcctggtg agcatcgtca
     1081 tcctgttcac ccgcccgccg gttcgtacga gcgtcaccaa ttgacttacc tccaaccgcc
     1141 ccgagatcgc gcgtccatga tcatggcacc aagaagagcc catcttgcga ccgatagccc
     1201 tagctccgag cccgggagcc gaggagcccg cgacgacggt ggcagcgtga tgaccgacct
     1261 cacgacgggt gcaccttatc ggagcccgtc acatcgaatc ctgaccggca gcgccggaga
     1321 cgggcatcgt tacggacgac ctaactgccg ccgatgaagc ctggcccgat gaggttccgc
     1381 caaggaagac atcgtcatgg gcggaagggg ctacgcccag gctcaagaag cgcttggagc
     1441 acggacacga gtttcgacac tgcgcaaggt tcggctaaat ccgccatcgc acatgaagcg
     1501 cctgtgggtc aagccgtcct acagcagaga agtacagcag catgtgccgt catcgatgac
     1561 gaacgaccag gagccgtgag ccttgcggca ccccttgggt tccgcgctga ggttgatctg
     1621 ccgccgagat cgcgcgagtt cttccatgtc gaagcagtgc cgtggaacgt tgcccggagg
     1681 gtgtccgctt gtgtacgcca gcgaccgcgg aaaggggctg ctgggacttc ggcgatcagc
     1741 ggtctagacg tcgtagagca ccgagacgca tcaggacgac gtgacgtcac ctcacgagta
     1801 cgcctttcac ctcatttgtg tgatgcgatg ccacagccgc ggtggcatcg tttgtccacg
     1861 tggcggcgac tgtcgatttg ggtccgcacc tcaacctgat catggcagaa cgtcattacg
     1921 cgggtcgcct ctgttcgaga gtcggcctcg aacctaggag aggcccatga ctgcaccaga
     1981 ggtttcacgt tcggtccttc cgatcccgca gcggccgcat gtggggctca ccacctacga
     2041 cgcgaaggat cctgacacgg cgttcccgcc gatcacgcct ctgcgcccgc cggagggggc
     2101 accgaacgtg ttgatcgttc tgatcgatga tgccggtttt ggttcctcca gcgcgttcgg
     2161 cggtccgtgc aataccccga cgttcgagcg gctggccggt ggggggctgc ggttcaaccg
     2221 gttccacacc acagcgcttt gctcgccgag ccgtcaggcg ctgttgaccg ggcgtaacca
     2281 tcacgcggtc ggcatgggcg gtatcaccga gatcgcgacg tccgcaccgg gctacaactc
     2341 gattcggccg gcgagcgcgg cgccgctggc cgagacgttg aagctgaacg ggtacagcac
     2401 cgcggcgttc gggaagtgcc atgaggtgcc ggtgtgggag accagcccga tgggtccatt
     2461 caatcagtgg ccgctgggca gcggcttcga gtacttctat ggcttcgtgg gtgccgagac
     2521 caaccagtgg tacccggcgc tctacgacgg tgtgacacca atcgagcctc cgaagacccc
     2581 cgaggagggc taccacctca ccgaggacct cgccgacaag gccattacct gggtgagtca
     2641 gcagaagtcg ctgatgcctg acaagccgtt cttcgtgtac ttcgcgccgg gtgcgaccca
     2701 cgccccgcac catgtcgccc aggagtggat cgagaggtac cggggccggt tcgaccaggg
     2761 ctgggacgcg atccgagagg agacgttcgc caggcagaag cagctcggcg tgatcccgcc
     2821 cgagacggag ctgtccgcca ggcccgcaca gatcgaggca tgggagaacg tgcctgatga
     2881 cctcaagccg gtgctcgccc gccaaatgga ggtgtacgcg gggttcctgg agcacaccga
     2941 ccaccaggtc gggcggctca tcgacaacct cgaggacctc gggattctcg acgacacgct
     3001 gatctacgtg atgatcggcg acaacggcgc cagcgccgag gggactacca ccggcacgtt
     3061 caacgagatc atctccctca acggtgccgc cgatctggag accactgagt tcatggtcgc
     3121 gcacatcgac gggttcgggt ccccggccgc gtacaaccac tatgccgtc
//
LOCUS       DASTXO010000429         3922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1204632, whole genome shotgun sequence.
ACCESSION   DASTXO010000429 DASTXO010000000
VERSION     DASTXO010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204632"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /locus_tag="VFU96_08985"
     CDS             complement(<1..1252)
                     /locus_tag="VFU96_08985"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEU5113440.1"
                     /translation="MGDFLLTVGFVLLFAWVARGLVGARELTWRRTLLAIFVGVTFGM
                     TVAILLLVRDFAEPQEIEGGQILALSLPFALIGTMGAIVVLELLFARPKARRGPHLPH
                     PFKALRRRAGIVRRGWQVTRIIARNGLAPLVGLRRGEVSTRSPDELARRVRIALEESG
                     GVFIKLGQLLVTRPDLLPPEALAELGRLHASAAPLPEDQVAQVIREETGREIEDVFTE
                     VDWDPLGSASIAQAHAARLRDGLAVVVKVRRPGLREQVERDLAIARWLARTAERRTTW
                     GAQYQVTSLVDEFSDVLHGELDFHVEARHAIEAGAAIDRHPRIVAPQIIQSLTSERML
                     VMERLPGVPLSADTGGIDPARGRLLADALCISQVQAMLKGERFHGDPHPGNVLVLESG
                     DLGLIDFGIAGRLDTFERASVFQML"
     gene            complement(1262..2044)
                     /locus_tag="VFU96_08990"
     CDS             complement(1262..2044)
                     /locus_tag="VFU96_08990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEU5113441.1"
                     /translation="MTVICDIDGVVYRGDQLLPGSDVALQRLVSAEVDFYFATNNSTK
                     TPLTVSERMFSMTGVAIAPESIVTSSEAAVGMLDEHEHPVMVLGSDGITGALADAGIG
                     VTDVPSEAKALLVGLDWDLSYERLTRAADAVRAGARFIATNTDPTYPVSGGVLPGGGA
                     MVAAVQTATGVIPEIAGKPHPPMRSLLRSRGIGAAWVIGDRVDTDIALARAEPDWTSI
                     LVLTGVTRPDESLGADHVVPDLAAAVDLVLAKGEETASSRVP"
     gene            complement(2041..3363)
                     /locus_tag="VFU96_08995"
     CDS             complement(2041..3363)
                     /locus_tag="VFU96_08995"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5113442.1"
                     /translation="MTPVSLLQELIRNQCVNDGSTHSGNEARSVSTLVDFIGTEGEVI
                     EPIPGRQSLVYRVPGFDPEAPSLALAPHLDVVPADPEGWSRDPFAAEIEDGFVYGRGA
                     VDMLNVTAAMAAAVRPYLTGEKKPRGDLLFAAVADEEAGGRLGAMHLVEEHWSLVAAD
                     YLLTEIAYPALELGGHRTVPVAIGEKGAYWSFLEARGTPGHGSLPYGADNAVEMMVSA
                     LAGLVSAPAPAAVTEEWLGFVEELGLDGDSVRRLTDIDQLDDEIDRISADDPAMARYI
                     HAATHLTISPNVISAGTKTNVIADKAHAEVDIRGLPGMDRDFVDAHLRKAMGTASDHI
                     EIVPVMDSEPTVSPVGNALWDAIGDAVEELDGHRNLAPTMMMAATDARFWRPKGTVCY
                     GVGLFDDRMGFSEMLSLFHGHDERVSAASVERTALLYERVLERFFTAP"
     gene            complement(3374..3781)
                     /locus_tag="VFU96_09000"
     CDS             complement(3374..3781)
                     /locus_tag="VFU96_09000"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU5113443.1"
                     /translation="MTNDELPLVGVGLVLVDDDRILLVQRGHEPSKGQWAVPGGKVNH
                     GETLRDAAIREVAEETGLTVEIGDVIWVGEHISEHGHIVLIDFLGSVVGGELTAGDDA
                     DRVEWVPLGVATSYQLTSTMYDLIDVLAVRAGS"
BASE COUNT          736 a         1307 c         1228 g          651 t
ORIGIN      
        1 ccagcatctg aaacaccgaa gctcgctcga acgtgtccag gcggcccgcg atgccgaaat
       61 cgatgagacc caggtcgcca ctctcgagga ccaagacatt gcccggatga gggtcgccat
      121 ggaatctctc ccccttgagc atggcttgga cctgagaaat gcagagggca tcagcgagca
      181 gccgaccgcg ggccgggtcg atcccgcccg tgtcggccga caggggcacg ccgggcaggc
      241 gctccatcac cagcatgcgc tcggatgtca aagattggat gatctggggg gcgacaatcc
      301 gcggatgacg atcgatggcc gccccggctt cgatggcatg acgagcctcg acgtggaaat
      361 ccaactcgcc gtggagcaca tccgagaatt catcgacgag tgacgtgacc tggtactggg
      421 ctccccaggt ggtgcgtcgc tctgcagttc tggccagcca ccgggctatc gccagatctc
      481 gttcgacttg ttctctcaga ccggggcgtc tcaccttgac cacgaccgca agcccgtcac
      541 ggagccgtgc tgcgtgtgcc tgcgcaatcg aggccgaccc cagaggatcc cagtcgacct
      601 ccgtgaacac gtcttcgatc tctcgaccgg tttcctcgcg tatcacctgt gccacctgat
      661 cctcgggcag cggcgcggca gaggcgtgaa gacgccccaa ctccgccagc gcctccggag
      721 gaaggagatc cggtcgcgtg accaggagct gaccaagctt gatgaagact cctcccgatt
      781 cctcgagagc gattcgaacc cggcgagcaa gctcgtcggg gctacgcgtt gagacctcac
      841 cccggcgcag gccgaccaat ggggcgagcc cgttgcgggc aatgatccgg gtcacctgcc
      901 agccgcgacg gacgatcccg gcgcgacgcc gcaatgcctt gaacggatga ggcagatgtg
      961 gcccgcgccg cgccttcggc cgggcgaata gcagctccag gacgactatc gcccccatgg
     1021 tgccgatcaa tgcgaagggc agcgacaggg cgaggatctg gcctccctcg atttcctgag
     1081 gctcggcgaa gtcccggacc aagagcagga tggccacggt cattccaaag gtgactccca
     1141 cgaagatggc gaggagggtt cgccgccagg tcaactcgcg ggcgcccacc aaacctcgag
     1201 ccacccacgc aaagagcagg acgaagccca cggtcaacaa gaaatcgccc atggcccaag
     1261 gctacgggac gcggctagaa gccgtttctt ctcctttggc caacacgagg tctacggctg
     1321 cggccaggtc ggggacgacg tgatcggcgc ccagcgactc gtcgggtctg gtgaccccgg
     1381 tgagaacgag gatggaggtc caatccggct cggcgcgggc caatgcgatg tcggtgtcga
     1441 cacgatcacc gatgacccat gccgctccga tgcccctcga ccggagcaaa gatctcatcg
     1501 gcggatgtgg ctttcctgct atctcgggga tgaccccggt ggcagtctgc accgcggcca
     1561 ccatcgctcc cccaccgggg aggacgccgc ccgagaccgg gtaggtgggg tcggtgttgg
     1621 tagctatgaa acgagctccg gcgcgaaccg cgtcggccgc cctggtcaat cgttcgtagc
     1681 tcaaatccca gtcgaggccg accagcagcg cctttgcctc gctcgggacg tcggtcaccc
     1741 cgatcccggc gtcggccagc gcgccggtga tcccgtcgga gccgaggacc atcaccggat
     1801 gttcgtgctc atcgagcatg cccacagccg cctccgagga ggtgacgatc gattcggggg
     1861 cgatggcgac accggtcatc gagaacatcc gctcgctgac agtgagcggg gtcttcgtgc
     1921 tgttgttcgt ggcgaaatag aaatccacct cggcgcttac gagacgttgc agtgcgacgt
     1981 ccgatccggg gagaagctgg tccccgcgat agacgacacc atcgatgtcg cagatgaccg
     2041 tcatggtgcg gtgaagaacc gctccaggac ccgctcatag agcagagccg ttcgctcgac
     2101 agaggccgcc gacaccctct catcgtggcc gtggaagaga gagagcatct cggagaaccc
     2161 catccggtcg tcgaagagcc cgactccgta gcagacggtt cccttcggtc gccagaagcg
     2221 cgcatcggtc gccgccatca tcatcgttgg cgcaagattc cgatgcccgt ccagctcctc
     2281 gacggcatct ccgattgcat cccagagcgc gttgccaacc ggtgagacgg tcggttcgct
     2341 gtccattacc ggcacgatct cgatgtgatc ggacgcggtc cccatcgcct tccggagatg
     2401 agcatcgacg aaatcgcggt ccatgcccgg gagcccgcga atgtcgacct cggcatgagc
     2461 tttgtcggca atgacattgg tcttggtccc tgcgctgatc acattgggcg agatcgtgag
     2521 atgcgttgcg gcatggatgt accgggccat cgccgggtcg tctgcggaga ttcgatcgat
     2581 ctcgtcgtcc aattgatcga tgtcggtgag acgacggacg gaatctccgt cgagacccaa
     2641 ctcttcgacg aagcccagcc actcctcggt cacggcggcc ggggctggag cgcttaccag
     2701 ccccgccaga gccgagacca tcatctcgac cgcattgtcc gcgccgtaag ggagtgatcc
     2761 gtgacctggg gtgccccgcg cttcgagaaa cgaccagtag gcgccctttt ccccgatcgc
     2821 gaccggcacg gtgcgatgcc cgcccaactc gagagccgga taggcgatct cagtcagcag
     2881 atagtcggcg gccaccaggc tccagtgctc ctcgaccaga tgcattgcgc cgagtcggcc
     2941 accagcctcc tcgtcggcga ccgcggcgaa caggaggtcg ccccgtggct tcttctcccc
     3001 ggtcagatac gggcgaacgg cggccgccat cgccgcggtg acgttgagca tgtccaccgc
     3061 accgcggccg tagacgaacc cgtcctcgat ctcggcggcg aaggggtccc gcgaccaacc
     3121 ctccgggtcg gccgggacga cgtccaggtg cggggccagc gcgagcgatg gtgcctcggg
     3181 gtcgaatccc ggcacccgat ataccagtga ctgcctgccc gggatgggct cgatcacctc
     3241 accctccgtt ccgatgaagt cgaccaacgt gctcaccgag cgcgcctcat tgccgctgtg
     3301 ggtcgaaccg tcgttcacgc attggttgcg gatcagctcc tgaaggagcg aaaccggcgt
     3361 catcggagtc agattatgac ccggcccgga cggcgaggac gtcgatcagg tcgtacatcg
     3421 tcgatgtcag ctggtagctg gtggccacac cgagaggaac ccactccacc cggtcggcgt
     3481 cgtcgcccgc agtcagctcg ccgccaacca cagaaccgag gaagtcgatc aggacgatgt
     3541 ggccgtgttc ggagatgtgc tcgccaaccc agatcacatc accgatctcc acggtgagac
     3601 ccgtctcctc ggcgacctcg cgaatcgcag cgtccctgag tgtctcgccg tggttcacct
     3661 ttccgccggg cacggcccac tgccccttgg aaggctcgtg acctcgctga accagcagta
     3721 tccgatcgtc gtcgaccagg accagaccga ccccgacgag tggaagctca tcgttcgtca
     3781 tcgtgatgca tgacgatcga gcgggccgcg aagcctcccg cttcgaagaa gttcttggcg
     3841 aggcgatgac cgggcaggac atgtgcgtcg aatttggaga tcccgcgccg acgcaggaga
     3901 tccatcacga actcacgcat ct
//
LOCUS       DASTXO010000430         2006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1204685, whole genome shotgun sequence.
ACCESSION   DASTXO010000430 DASTXO010000000
VERSION     DASTXO010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204685"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="VFU96_09005"
     CDS             complement(<1..1181)
                     /locus_tag="VFU96_09005"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019855026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEU5113444.1"
                     /translation="MTTDTAPKATSPGRTAPRERPAARPRRKKGEGQWALGYREPLNP
                     NEQSKKDDNPLNVRARIENIYAKRGFASIDPNDLRGRFRWYGLYTQRRPGIDGGKTAT
                     LEPWELDDEYFMLRIRIDGGALTTEQLRVIGQISVDFARDTADVTDRQNVQLHWIRIE
                     DVPEIWRRLEAVGLHTTEACGDCPRVILGSPVAGIAAEEIIDPTPAIREIADRYIGDP
                     EYANLPRKFKTALTGHPVHDVAPEVNDVAFVGVVHPEHGPGFDLWVGGGLSTNPMLAV
                     RLGTWVPREEVAEVWAGVVAVFRDYGYRRLRTRARIKFLVADWGAEKFRQVLETEYLR
                     RRLIDGPAPGVPTEPGDHVGIHRQKDGKYFVGLSPVAGRVSGSNLVRLADLAETHGSR
                     R"
     gene            complement(1484..>2006)
                     /locus_tag="VFU96_09010"
     CDS             complement(1484..>2006)
                     /locus_tag="VFU96_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU5113445.1"
                     /translation="LVFLVAGIVLLPLILAGPSLVSRIVPPGLEFIGSLYWPVVLLGS
                     VFMLASLYHLSVPTRTRWRAALPGAAITVAMWVGGSVLLRLVLSKSVGSPTIYGPLAA
                     PVAFLIWLYVMSIAVLIGAAFNAGLDDVFPRLSGIDHDAPGTEKPAANNMEGNPLRRT
                     LAGVRARRRVKSQ"
BASE COUNT          392 a          610 c          655 g          349 t
ORIGIN      
        1 atgcgacgtg aaccgtgcgt ctcggccagg tcagccagcc gcaccaggtt gctgccgctg
       61 acccgcccag cgactgggga cagcccgaca aaatacttac cgtccttttg acggtgaata
      121 ccgacatgat caccaggttc ggtcggcact cctggcgccg gcccatcaat caggcgccgc
      181 ctcaggtact cggtctcgag tacctgacgg aacttctctg caccccagtc ggcgaccagg
      241 aacttgatcc gggctcgggt ccggagtctg cgatagccgt aatcgcgaaa cacggccaca
      301 acgccggccc agacctcggc cacctcttct cggggcaccc atgtcccgag gcgtacggcg
      361 agcatcggat tggtggaaag gccgccgccg acccacaggt cgaatcccgg tccgtgttcg
      421 ggatgtacaa caccgacaaa ggcgacatcg ttgacctctg gtgcgacgtc gtgaacggga
      481 tgaccggtga gagcagtctt gaacttcctc ggcaggttgg catactccgg gtcgccgatg
      541 tagcggtccg cgatctcccg aatggctggt gtgggatcga tgatctcctc agcagcaatc
      601 ccggcaaccg gagagccgag gatcacgcgc ggacagtcgc cacaggcctc ggtggtatgg
      661 agtccgaccg cctcgaggcg acgccaaatt tcgggaacgt cctcgattcg aatccaatgc
      721 aattggacat tctgccggtc cgtgacatcg gcggtgtccc tcgcgaaatc gaccgaaatc
      781 tggccgatga ctcgcagttg ctcagtcgtc aaggcgccgc cgtcgatgcg gatgcgcagc
      841 atgaagtact catcgtccag ctcccacggc tccagagtcg cggtcttgcc tccatcgatg
      901 ccaggtcggc gctgggtgta gagcccgtac cagcgaaagc gcccacggag gtcattggga
      961 tcaatcgagg cgaagccacg cttggcgtag atgttctcga tccgtgcccg aacgttgagc
     1021 gggttgtcgt ccttctttga ttgctcgttg gggttcagcg gctcgcgata gccaagggcc
     1081 cactgtcctt cgcccttttt gcgacgcggt cgggcagctg gccgttctct aggggcagtg
     1141 cggcccgggc tagttgcctt gggtgcagtg tcggtggtca tgtgggtgtc cccgttctcg
     1201 gagcagcacg agcggtctgg cgggttggac ggtggatgcc aggtgggcgg ctgcgggcca
     1261 gtggatcagg cagcgtcgag gtgacacatc atcgactcgg tccggccgag atcgacgaag
     1321 cgccgcgcag tcaaacccaa ggtccgacga gcaggcgaga gcgccggtcg cgccggcaga
     1381 ggccagacgc ggatagtttc cggagcgata cgcacggacc aacactccgc tagtgacctt
     1441 gatcgaatca aatcgtttct cactatgtgg gaatggcatc caattattgg gatttgacgc
     1501 gtcggcgggc acgtacacca gcgagcgtgc ggcgtagcgg gtttccctcc atgttgttcg
     1561 cggcaggttt ctcggtgcca ggagcatcat gatcgatgcc cgacagccgg gggaaaacgt
     1621 cgtcgaggcc ggcgttgaag gccgcaccga tcagcacggc gatggacatc acgtagagcc
     1681 agatcaggaa tgcgacggga gcggccagcg ggccgtagat cgtggggctc ccgacggatt
     1741 tgctcaacac gagccgcaag agcacgcttc cgccgaccca catggccaca gtgatggccg
     1801 cgccgggcaa agctgccctc cagcgggtac gagtcggaac cgacaggtga tagagcgagg
     1861 ccagcatgaa gacggagccc agcaacacga cgggccaata caggctgccg atgaactcga
     1921 ggcctggtgg aactatccga cttaccaggc tgggaccggc caggatgagg ggcagcaaca
     1981 cgatcccggc gacgaggaag accaga
//
LOCUS       DASTXO010000431        10138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_209557, whole genome shotgun sequence.
ACCESSION   DASTXO010000431 DASTXO010000000
VERSION     DASTXO010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 10138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10138
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209557"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VFU96_09015"
     CDS             complement(<1..421)
                     /locus_tag="VFU96_09015"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEU5113446.1"
                     /translation="MSVRRWPALDGLRGFAILFVLAAHSAIVNVYVAGQVGVTLFFVL
                     SGFLITYLLLDEVDDTGGVDLKAFYGRRALRLLPALVVYLVGIAILMSFLDLALPIWD
                     MTWPPAVYLANYAQILGQDLYAHRHLWSLAVEEHFYLV"
     gene            complement(517..2523)
                     /gene="uvrB"
                     /locus_tag="VFU96_09020"
     CDS             complement(517..2523)
                     /gene="uvrB"
                     /locus_tag="VFU96_09020"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011605724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEU5113447.1"
                     /translation="MPDFKIVSDFAPAGDQPEAIAALAEGIERGDRYQTLLGITGSGK
                     SATIAWTIEQVQKPTLVLAPNKALAAQLANEFRQFFPHNRVEYFVSYYDYYQPEAYMP
                     QTDTYIEKDATINDEIDRLRHSATAGLLVRNDVIIVASVSAIYGLGSPEQYMGQLLRL
                     IQGVEYPMKDAIQRLVDIQYERNEVNLIRGKFRVKGDTLEVFPSYEETILRVQYWGDE
                     VERLIKLNPVTGEVIDELSEAYVFPATHYVTERERMKKAIAGIEVELEERLTELERKG
                     KMLEAQRLRMRTNYDLEMMREVGFCSGIENYSRYLDGRSEGEAPYTLLDYFPKNYLTI
                     VDESHVTVPQIGGQYEGDHSRKEVLVEHGFRLPSAKDNRPLRFDEWLERANQVVFLSA
                     TPSDFETSRSTQVVEQIIRPTGLIDPEVMIRPTKGQIDDLLHEIRERALREQRVLVTT
                     LTKKMAEDLTDYLLEMGVRARYMHSEIDTLERVQIMRELRLGDFDVLVGINLLREGLD
                     LPEVSLVAILDADKEGFLRSETSLIQIIGRAARNVDGQVVMYADNVTESMKRAINETN
                     RRRKAQSEWNAEHGIDPQTVRKRVSDILELVQSEAPKGDRRRREVEPRRPIDLEGDDL
                     TRLIMSLEDEMHEAAKELRFEYAARLRDEVNELRRELREVAEAG"
     gene            complement(2563..3132)
                     /gene="coaE"
                     /locus_tag="VFU96_09025"
     CDS             complement(2563..3132)
                     /gene="coaE"
                     /locus_tag="VFU96_09025"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU5113448.1"
                     /translation="MSSSDGRWLLTGGIGSGKSEVRRLLADRGIHTIDADAVGHAVLA
                     GEGFAPVAKRWPGVVLEGQIDRKTLAEIVFADAGELRALEAITHPLIFGRIEGDLEGF
                     SGVAVVEMPVLAPTLAWPMIVVDAEDEIRVRRAMQRGMDEPDIRRRMASQASRAEWLA
                     AADLVVPNHGDLRQLEATVGVLGDYLEGA"
     gene            3158..4678
                     /locus_tag="VFU96_09030"
     CDS             3158..4678
                     /locus_tag="VFU96_09030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU5113449.1"
                     /translation="MTVSVPAPWEFRLHRSVTIGQFVVLIYAVVFDLISASGSPDGQS
                     LLAMALGTIYVLGTAAVPESWYRLRFGAEAITLIGAFLTITAITLTGGPNSPYILLSM
                     GPPILATVYGGLRTGLMTGFLSVALLILVTISQGDSGAAITDALPVIGLYVAFVLLVT
                     VIRRLLEDAHRQAGELAEATESATQKLENLEEIHGVLIRLSEDISAGRLNAVEVAADT
                     LEAILARIPGSSGRLVLEDEEGPVVLASRGVPPESPHPYQVPLSTADASVGSLELLTP
                     QSLSRPELIDIEAFVQPVSIAFANLQLLQSIVGSAVAEERIRLAREMHDDIGPSLASL
                     GLALDMAAMQQAEHAELSADLKVLRTNVTKLVEDVRASVADLRTAPGPTLTARLLQST
                     SGLDGTPQVVVDLEERRPPRPAVIADLTSIITEATRNAHNHSGGSQILVSGRIDRSFG
                     ICSVIDDGTGFDPTYEPDGHFGLTGMRERADKIGAKISFQPTPGGGTTVTVEWGNR"
     gene            4687..5313
                     /locus_tag="VFU96_09035"
     CDS             4687..5313
                     /locus_tag="VFU96_09035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5113450.1"
                     /translation="MIADDHQLFAEGLSQALNILPDTRVVGVVASGPALEEALMGQPA
                     EVSIVDIEMPGGDGIDTISELGRRTGAIVVSMHASDEQKERAQKAGAKGFFSKGVPLV
                     TLAAAVRAVADGQDLISLDDTARDELLEHYGQARLDPGAASLTEREKEILQLLAVGVS
                     ATDELAERLYISQKTVKNHLASIFQKLAVSDRTQAAIEAIRLGIARPS"
     gene            5392..5586
                     /locus_tag="VFU96_09040"
     CDS             5392..5586
                     /locus_tag="VFU96_09040"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEU5113451.1"
                     /translation="MSKGVEMYARLMRFLSNEKGASLVEYSLLVLLIAIAALAAVAYF
                     GDELSSTYSDISSNLVDAGS"
     gene            5677..5850
                     /locus_tag="VFU96_09045"
     CDS             5677..5850
                     /locus_tag="VFU96_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEU5113452.1"
                     /translation="MFALWTKLTSWMAEEKGASMVEYALLVVLIAIVALVAVRLAGRE
                     VSSTFSEIGSGLT"
     gene            5967..6143
                     /locus_tag="VFU96_09050"
     CDS             5967..6143
                     /locus_tag="VFU96_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEU5113453.1"
                     /translation="MFALWTKLTSWMAEEKGASMVEYALLVVLIAIVALVAVRLAGNE
                     VSSTFSEIGSGLGS"
     gene            6270..6443
                     /locus_tag="VFU96_09055"
     CDS             6270..6443
                     /locus_tag="VFU96_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEU5113454.1"
                     /translation="MLALWTKVVSWMQEEKGASMVEYALLVVLIAIVALVAVRLAGRE
                     VSSTFSEIGSGLT"
     gene            6589..6762
                     /locus_tag="VFU96_09060"
     CDS             6589..6762
                     /locus_tag="VFU96_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEU5113455.1"
                     /translation="MSRLWVKTSNWMAEEKGASMVEYALLVVLIAIVAILAIRLAGNQ
                     VSEAFSEIGSGLQ"
     gene            complement(6769..8043)
                     /locus_tag="VFU96_09065"
     CDS             complement(6769..8043)
                     /locus_tag="VFU96_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113456.1"
                     /translation="MALVIDAWAPIRDNSFLWHIRAGELQASAGEVLVADPFSFTMLG
                     ERWLTQSWLAELLYSWGESNWGLGFVPPMMLLLSSITFLGIGVVSYRRSRSEWSTAII
                     VALTTLLMISFLVPRPVLFSLALFALVVVAWDRPVTRWTVPFLFWIWASVHGSFAIGL
                     AYVGLTIIVERDWKALPTAIVAGLSTFLTAHGLGVVEFLLDFSEAGPALALLSEWRRP
                     ELLSVVFAPFLIGVGLIVIGAFRGKVTPKHLIILVPFLLLALSATRAVPPAWIALVPL
                     MATALAGLAVGARQRFSRAAAVVFGVVVLVVPWFIISDGSIDEEKFPVAASEELADVP
                     TFHDDSAGGYLIWAEGPERLVYIDDRAELYGDRMLEYVEVRDLERDWEPVFERDGIEQ
                     VLLKEDEELIGELEDAGWSTVYDEGPYVVMRP"
     gene            complement(8115..9056)
                     /locus_tag="VFU96_09070"
     CDS             complement(8115..9056)
                     /locus_tag="VFU96_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU5113457.1"
                     /translation="MNVTALDGQMLAVVTVSALVFFVIVWIGLGVTRAREDMNNRLAV
                     YGRTTAGTVRDDELAKPLAQRTIGPIVLKISGFLRRFTPVGYLEKKQHQIVLAGNPGN
                     LDAPSFIVIKLLTTIAGLFAAFFLTDFGSDSLQRIILFAMPIALGFFGPDAWLQRKID
                     DRRQNMLRALPDVLDLLVISVEAGLGFDSALSRVVATVPGPLSEEFFRMLQETRVGVS
                     RRDAMRHLMDRTDLDELRSFILAMIQAEAFGVTIARVLRVQADEMRVKRRQRAQEKAF
                     AAPVKLVFPLVFCIFPALFIVLLGPAAISIAGAFSST"
     gene            complement(9053..>10138)
                     /locus_tag="VFU96_09075"
     CDS             complement(9053..>10138)
                     /locus_tag="VFU96_09075"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU5113458.1"
                     /translation="VVVPVSGYATTTTIAPTTTTVPVPITGSVVESNSPLSITNLALI
                     AAAGIGLTAFLFIVILFGRDEEQGGGRFAKRLQAYGRRSRSGEEEKKPFLQRIPVLNR
                     FSQAAEEEVRKRGLLSGVNAALEQANIPMSPGEAILAMFGLSAVGGVFMAIFSSPLVG
                     LITFGVLLLAFAMLMKYAGNRERKKFEKQLPDTLTLLSTSLRAGYSLLQAIEAVASEA
                     PDPTAREFSRAVVEARLGRSVPEALDGIVSRTSSKDFEWATMAIEIQREVGGNLAEVL
                     QTVADTMMARNRLKGEIKALTAEGRISAIVLGSLPFALAGFLWFNNREYLQPLLDATL
                     GRIAIVAGLVLMAGGIFWLRKIVNIEV"
BASE COUNT         1960 a         3190 c         3118 g         1870 t
ORIGIN      
        1 acaccaggta gaaatgttct tcgaccgcca ggctccagag atggcgatgg gcgtagaggt
       61 cctgaccgag gatctgggcg tagttggcca ggtagacggc tgggggccag gtcatgtccc
      121 agatcgggag cgccaaatcg aggaaggaca tgaggatcgc tatcccgacg aggtagacca
      181 ccagggccgg cagcagtcgg agggcacgcc gcccatagaa cgccttcagg tcgactcccc
      241 cggtgtcgtc gacctcgtcg agtaggaggt acgtgatcag gaatccggag aggacgaaga
      301 acaaggtgac gccaacctgt cccgcgacgt agacgttgac gatcgcggaa tgtgcggcga
      361 gcacgaacag gattgcgaat cccctcaacc cgtcgagggc gggccacctt ctcacagaca
      421 tatatcggga ctatcgcctc gaacattgag cgactgaaga cgaaccctga gccaggaaat
      481 atcgccgttt gacggcccgc cccagccgat gagggctcag ccggcctcgg cgacctcacg
      541 aagctcacga cgtagctcgt tgacttcgtc gcggagccgg gcggcgtatt cgaatcggag
      601 ttccttggca gcctcatgca tctcgtcttc caggctcatg atcagcctgg tgagatcgtc
      661 cccttcgagg tcgatcggcc ggcgtggctc gacctcgcgt cgtcgtcgat ccccctttgg
      721 agcctcggac tgaacgagct ccaatatgtc ggagacgcgt tttctcaccg tttgagggtc
      781 gatcccgtgc tccgcgttcc attccgactg tgctttgcga cgtcgattgg tctcgttgat
      841 cgcccgcttc atcgattcgg tcacattgtc ggcatacatg accacctgac cgtcgacatt
      901 ccgagccgcc cggccgatga tctggataag cgaggtctcc gagcggagga agccctcttt
      961 gtcggcgtcg agaatcgcca ccagcgacac ctcgggtagg tccagccctt cacggaggag
     1021 gttgatcccg acgagcacat cgaaatcgcc cagtcgcagc tcgcgcatga tctgcacccg
     1081 ttccaacgtg tcgatctcgg aatgcatgta gcgagccctc acccccatct ccaggaggta
     1141 gtcggtgaga tcttccgcca ttttcttggt cagagtcgtg accagcacac gttgttcccg
     1201 gagagctcgc tcccttatct cgtgaaggag gtcgtcgatc tggcccttgg ttggccggat
     1261 catcacctcg gggtcgatca gaccggtggg gcgaatgatt tgctcgacca cctgagtcga
     1321 tcgagaggtc tcgaaatccg agggtgttgc ggagaggaag acgacctgat tggccctctc
     1381 cagccattcg tcgaaccgta gtgggcggtt gtctttggcc gaaggaagcc gaaacccatg
     1441 ttcgacgagg acctccttgc gtgagtggtc accctcgtac tgcccgccga tctgggggac
     1501 ggtcacgtga gactcgtcga cgatggtcag atagttcttg gggaagtaat cgagcagggt
     1561 gtacggcgct tctccctcgg atcttccatc gagatagcgt gagtagttct cgatcccgga
     1621 gcaaaagccg acctcacgca tcatctcgag gtcgtagttg gtccgcatgc gaagccgctg
     1681 ggcttcgagc atcttgccct tccgttccag ctcggtgagc cgctcctcga gctcgacctc
     1741 gatgcccgcg atcgccttct tcatgcgctc ccgctcggtg acgtagtggg tggcgggaaa
     1801 gacataagcc tccgagagct cgtcgatgac ctcgccggtc accgggttga gcttgatcag
     1861 cctctcgacc tcgtcccccc aatattgaac tcggaggatt gtctcctcat aagagggaaa
     1921 gacctcgagg gtgtctccct tgacccggaa ctttccccgg atgagattga cctcgttgcg
     1981 ctcgtactgg atgtcgacca gacgctggat tgcgtccttc atcgggtact ccacgccctg
     2041 gatcaggcga agcagctgac ccatgtactg ctcgggcgag cccaagccat agatggctga
     2101 caccgaagcg acgatgatca cgtcgttccg aaccaggaga ccggccgtcg ccgagtggcg
     2161 gagacgatcg atctcatcgt tgatcgttgc gtctttctcg atgtaggtat cggtctgcgg
     2221 catgtatgcc tcgggctggt agtagtcgta gtaggagacg aagtactcga cccggttgtg
     2281 tgggaagaac tggcggaact cattggcgag ctgggcggcg agcgccttgt tgggcgccag
     2341 gacgagtgtc ggtttctgca cctgctcgat cgtccaggcg atggtggccg acttcccgga
     2401 tccggtgatc cccaacaggg tctgatagcg atcgcctctc tctatcccct cggccagggc
     2461 cgcgatcgcc tctggctggt caccagccgg ggcgaagtca gaaacgatct tgaaatcggg
     2521 cacgttcgga gggtaccggt ggccagggac aagatccgtc cgctatgcgc cttcgagata
     2581 atcacccagt acgccaaccg tggcctccag ctggcgcaga tccccgtgat tggggacgac
     2641 gaggtccgcc gccgcgagcc actccgcgcg gctcgcttga gaagccattc gacgacggat
     2701 atctggttca tccatccccc gctgcatagc ccggcgcacc cggatctcgt cctcggcgtc
     2761 caccacgatc atcggccaag ccaaggtggg ggccagcacc ggcatctcga cgactgccac
     2821 tccggaaaac ccctccaaat ccccctcgat tcgaccgaag atcaagggat gagtgatcgc
     2881 ttccagcgcc ctgagctcgc ccgcgtcggc gaaaacgatc tcagccagcg tcttgcggtc
     2941 tatctggcct tcgaggacga cgcccggcca tcgcttggcg accggggcga agccttcacc
     3001 agccaggacc gcatgaccca cggcgtcagc gtcgatggtg tgaatgcccc tgtccgccaa
     3061 tagccggcga acctcgctct tgccagagcc gatcccgccg gtgaggagcc agcgcccgtc
     3121 cgaggaactc atcgcctccg acggtagcct tgccccaatg acggtctccg tcccggcccc
     3181 ctgggaattc cgcctccatc ggagtgtcac catcgggcaa ttcgtcgtcc tgatctacgc
     3241 ggtcgtattc gatttgatct ccgcgagcgg atcccctgac ggccagtcgc tcctggcgat
     3301 ggccctgggc acgatctatg tcctggggac ggcggcggtc ccggagagct ggtatcgcct
     3361 gcgctttggc gctgaggcga tcaccctgat cggtgccttc ctcaccatca ccgccatcac
     3421 tctcaccggg ggacccaaca gcccctacat cttgttgtcg atggggccgc cgattcttgc
     3481 cacggtctac ggcggtctcc gcaccgggtt gatgaccggt tttctctcgg tggcactgct
     3541 gattctggtc actatctcgc agggagactc cggagctgcg atcaccgatg cattgcccgt
     3601 cattggcctc tacgtcgctt ttgtgttgct ggtgacagtg atcaggcgtc tcctcgagga
     3661 cgcccatcgt caggccggcg agctggccga agcaacggaa tcggcaacac aaaaactgga
     3721 gaatctcgag gagatccacg gcgtcctcat ccggctctcc gaagacatct cggcggggcg
     3781 cctcaacgcc gtcgaggtgg ccgccgacac actcgaggcc attctcgccc gcataccggg
     3841 ctcgtccggg cgtctcgttc tcgaagacga agaaggcccc gtggtccttg cctccagggg
     3901 cgtgcccccc gagagccctc acccctatca ggtcccgctg tcgacagccg acgcttcggt
     3961 ggggagtctg gagctcctca ccccccaatc gttgtcgaga ccggagctca tcgacatcga
     4021 ggctttcgtc caaccggtga gtatcgcctt cgccaacctc cagctgctgc agagcatcgt
     4081 cggatcggcc gtcgccgagg agcggatacg tctggccagg gagatgcacg acgacatcgg
     4141 gccttcactg gcgtcactgg gcctggcact ggacatggcg gcgatgcagc aggctgagca
     4201 cgccgagctg tccgccgatc tcaaggtatt gcgcaccaac gtcaccaaat tggtcgaaga
     4261 cgtgcgggcc tccgtcgccg atctacgcac cgccccaggc ccgaccctga cggctcgact
     4321 cctccaatcg acatctggct tggacggaac gccccaagtc gtcgtcgacc tggaggagag
     4381 gcgacctcct cgacccgcag tgatcgccga cctgacatcg ataatcaccg aggccacccg
     4441 taatgcccac aatcactcgg gtgggtcaca gatactggtg tcggggagaa tcgaccgctc
     4501 tttcgggatt tgctcggtca tcgacgacgg caccggtttc gacccgacct acgagcccga
     4561 tggccatttc ggcctcacgg ggatgcggga acgtgccgac aagatagggg ccaagatcag
     4621 ctttcagcca actcccggag gaggaacgac cgtgactgtg gaatggggca accgatgatt
     4681 cgcgtgatga tcgccgacga ccaccaactg ttcgcagaag ggctgagtca ggccctcaac
     4741 atccttcctg atactcgagt ggtcggggtg gtggcgtccg ggcctgcact tgaggaagcc
     4801 ctgatgggac agccggcgga ggtttccatc gtcgacatcg agatgcccgg tggcgacggg
     4861 atcgacacca tttccgagct ggggaggcgc accggcgcca tcgtcgtctc gatgcatgct
     4921 tcggacgagc agaaagagcg cgcccagaaa gcgggcgcca agggcttctt ctccaagggg
     4981 gtgccgctgg tgactctcgc cgctgcggtc agggcagtgg ccgacggcca ggatctcatc
     5041 tcactcgacg acacggcccg agacgagctc ttggagcact acgggcaggc ccgcctcgac
     5101 ccgggagcgg cctcgctcac cgaacgggag aaggagatcc tccagctcct ggcagtcggg
     5161 gtatccgcca ccgacgagtt ggccgagcgt ctgtacatct ctcagaagac ggtcaagaac
     5221 cacctcgcat cgatcttcca gaagctcgcc gtcagcgatc gcacccaggc cgccatcgag
     5281 gcgatacgcc tgggcatcgc acggccgagc tgaccaatga gaacgggcca aatgccatat
     5341 gagccttgag gcccatgagc gttttccggc ttcggcccta cgctgatgcg ggtgagcaag
     5401 ggagtagaaa tgtacgccag gttgatgagg tttctcagca acgagaaggg tgcttctctg
     5461 gttgagtact cgctcctcgt cctcctcata gccattgcgg cccttgccgc cgtggcctac
     5521 ttcggagatg agctcagcag cacatactcc gacatcagtt cgaatctggt ggacgcgggg
     5581 tcgtaacgat agttcccggg acccataggt cccgggggta ggtagggcta ccttgcccga
     5641 ttgcaagcca aggcaactaa cccaaggagg ggttccatgt ttgctctgtg gaccaagttg
     5701 acgagctgga tggctgagga gaagggcgcc tcgatggttg agtatgccct gctcgtcgtc
     5761 ctgatcgcca ttgtcgctct cgtggccgtg cgtctcgccg gtcgtgaggt ctcttcgacc
     5821 ttcagcgaga tcggttctgg cctcacctga gagtcaacga ctcgacagaa aagggcccgg
     5881 ttttccgggc ccttttcaat ttctcctcag gtatcgggct gatctgtcga catctcacca
     5941 gtacgcatac tcaaaggaaa agcttcatgt ttgcattgtg gaccaagttg acgagctgga
     6001 tggctgagga gaagggcgcc tcgatggttg agtacgccct gctcgtcgtc ctgatcgcca
     6061 tcgtcgccct cgtcgccgtg cgtctcgccg gcaatgaggt ctcctcgacc ttcagcgaga
     6121 tcggctccgg cctcggtagc tgagagtcaa cgactcgaca gaaagggccc ggttttccgg
     6181 gcccttttct cttttttccc cttaggtttt gggcgcatcg gccgataaat cacatgcaag
     6241 ccgtattcgc agacccaagg aggggcttca tgcttgcttt gtggaccaag gtagtgagct
     6301 ggatgcagga ggagaagggc gcctcgatgg ttgagtacgc cctgctcgtc gtcctgatcg
     6361 ccattgtcgc tctcgtggcc gtgcgtctcg ccggtcgtga ggtctcttcg accttcagcg
     6421 agatcggctc cggcctcacc tgagctcagc tcagatcaaa cgaagaagga cccgcctcgc
     6481 gggtcctttt tctatttccg tgccataggc ctatcgactc attgcccggt cgccgatgcc
     6541 gacgtaccgt cctttgagaa agacacgacg aacgaccagg gaggcatcgt gtcacggctc
     6601 tgggtcaaga catccaattg gatggccgaa gagaagggcg cttcgatggt ggaatacgcc
     6661 ctcttggtag tgctgatcgc catcgtcgcc atcttggcca taagactcgc cggcaaccag
     6721 gtttccgagg cgttcagcga gatcggatcg ggtctccagt agcggcggtc acggtctcat
     6781 gacgacgtag gggccctcgt catagacggt cgaccaaccc gcgtcctcca gctcgccgat
     6841 cagttcctca tcctccttga gcagcacctg ctcgatcccg tcacgttcga acaccggctc
     6901 ccagtcccgc tcgagatccc tcacctcgac gtactcgagc atcctgtccc cgtagagctc
     6961 ggcccggtcg tcgatgtaga ccagacgctc cggcccttcg gcccagatca ggtacccgcc
     7021 cgcactgtcg tcgtggaatg tcgggacatc ggccagctcc tcgctcgccg cgacgggaaa
     7081 cttctcctcg tcgatagaac cgtccgagat gatgaaccac ggtacgacaa ggaccacgac
     7141 gccaaatacc accgccgctg ccctggaaaa gcgctggcgc gccccgaccg cgagaccggc
     7201 caacgccgtg gccatgagcg gcacgagtgc gatccaggcc ggtgggactg ccctggtggc
     7261 gctcagggcg agcaatagga acgggaccag gattatcaga tgcttgggtg tgacctttcc
     7321 acggaatgcc ccgatgacga tcagcccgac acctatcagg aacggggcga agaccacgga
     7381 cagcaactcg ggccgccgcc attcggagag tagggccagg gcggggccgg cctcggagaa
     7441 gtcgagaagg aactcgacaa cacccaggcc atgtgcggtg aggaatgtgc tcagaccggc
     7501 cacgatcgcc gtgggaagcg ccttccagtc cctttcgacg atgatcgtca gtccgacgta
     7561 ggccagccct atggcaaagc tcccgtgcac tgaggcccag atccaaaaca ggaagggaac
     7621 cgtccatcgc gtcaccgggc gatcccaggc gaccacgacc aacgcaaaca gagcgagtga
     7681 gaacaacacc ggtctcggca ccagaaacga gatcatgagc aaagtggtga gggcgacgat
     7741 gatcgcagtc gaccactcac tcctcgaacg tcggtaagac acgacgccga tgccgagaaa
     7801 cgtgatcgag ctgaggagca gcatcatcgg tggcacgaag ccgagacccc agttcgattc
     7861 tccccagcta tagagcagct cggcgagcca gctctgggtg agccagcgct cccccagcat
     7921 ggtgaacgag aacggatcgg cgacgaggac ctccccggca ctcgcctgca gctcgccggc
     7981 cctgatgtgc cacaggaagg agttgtcccg gatcggcgcc caggcgtcga tgacgagtgc
     8041 cacgaacggc acgaccagaa cgaggtgcgc gagagagaat cgccgttgag acggctccgg
     8101 cgccgccggg tccatcaggt cgaggagaag gcgcccgcga tcgagatcgc ggccggaccg
     8161 aggagcacga tgaacaacgc cgggaagatg cagaacacca aggggaagac cagcttcacc
     8221 ggtgcggcga atgccttctc ctgagcccgc tggcggcgct tcactcgcat ctcatcggcc
     8281 tgcaccctga gcacccgtgc gatggtcaca ccaaatgcct ccgcctggat catggccagg
     8341 atgaacgacc gaagctcgtc gaggtccgtt cgatccatca gatgcctcat cgcatcgcgg
     8401 cggctgactc cgacccgggt ctcctggagc atcctgaaga actcctcgga gagcggaccg
     8461 ggcacggtcg ccaccacgcg ggacagagcc gagtcgaagc ccagaccggc ctcgaccgag
     8521 atgacgagga ggtcgaggac gtcggggagg gcccggagca tgttctgacg gcgatcgtcg
     8581 atcttgcgct gcagccaggc gtcgggcccg aagaatccga gcgcgatggg catggcgaag
     8641 aggatgatgc gttgcaggct gtcactgccg aaatcggtca ggaagaaggc ggcgaacaga
     8701 ccggcgatgg tcgtcaacag cttgatgacg atgaatgagg gggcatccag gttgcccggg
     8761 ttgccggcga ggacgatctg gtgctgcttc ttctccaggt acccgaccgg ggtaaagcga
     8821 cgcaggaaac ctgagatctt gagcacgatc ggcccgatcg tccgctgagc caggggcttg
     8881 gccagctcgt cgtcacgcac cgtcccagcg gtggtacgac cgtagacggc cagcctgttg
     8941 ttcatgtctt cacgggcccg ggtcactccg aggccaatcc aaacgatgac gaagaagacc
     9001 agcgccgaga ccgtgaccac ggcgagcatc tgaccgtcta gtgctgtgac gttcatactt
     9061 cgatgttcac aatcttccgg agccagaaaa ttccaccggc cattaggacg agaccagcga
     9121 cgatggcgat tcttcccaac gtcgcatcga gcaagggctg caggtattcg cggttgttga
     9181 accacaggaa cccggccagt gcaaagggca gagagccgag cacgatcgcc gagatccggc
     9241 cttctgcggt caaggccttg atctcaccct tgagccggtt gcgggccatc atggtgtccg
     9301 ccaccgtctg caggacctcg gccaggttgc caccgacctc acgctggatc tcgatggcca
     9361 tcgtggccca ctcgaagtcc ttggagctgg tccgagagac gattccgtcg agcgcctcgg
     9421 gcacagagcg acccagcctg gcctcgacga ccgctcggct gaattcgcgg gcggtcgggt
     9481 ccggcgcttc ggaggccacg gcttctatgg cttggaggag cgagtaaccg gcacgcaatg
     9541 aggtggagag gagtgtcaag gtgtccggca actgcttctc gaacttcttc cgctcgcggt
     9601 tgccggcata tttcatcagc atggcgaagg ccaggagaag cactccgaat gtgatgaggc
     9661 cgaccagcgg ggagctgaag atggccatga agaccccgcc caccgcggag agaccgaaca
     9721 tggcgagaat ggcctcgccc ggcgacatcg ggatgttggc ttgctccagg gcggcattca
     9781 ccccagagag caggccgcgc ttccgcactt cctcctcagc ggcttggctg aaccggttga
     9841 ggacgggaat acgctgcagg aagggctttt tctcctcctc ccccgaccgc gaccggcgac
     9901 cgtaggcctg gagccgcttg gcgaaccgcc cgccaccctg ctcctcatcg cgcccgaaca
     9961 gaatgacgat gaacagaaag gcggtgagac cgatcccggc cgcagcaatc agggccaggt
    10021 tggtgatgct cagcggagaa ttgctctcga cgacactgcc ggtgatcgga acggggaccg
    10081 tggtcgtcgt aggtgcaatg gtcgtggtcg tggcatatcc ggagaccgga accacgac
//
LOCUS       DASTXO010000432         3423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1499364, whole genome shotgun sequence.
ACCESSION   DASTXO010000432 DASTXO010000000
VERSION     DASTXO010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499364"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VFU96_09080"
     CDS             complement(<1..287)
                     /locus_tag="VFU96_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113459.1"
                     /translation="MRFPDDYMGEMATTKVLDWEIERLLAGTPVAGGRLSGLVPVVEM
                     VRTQWVGRPSESAVQRFAVGASSVLRATRPEASELLKLQRRRVGGRLGLSMR"
     gene            complement(284..865)
                     /locus_tag="VFU96_09085"
     CDS             complement(284..865)
                     /locus_tag="VFU96_09085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU5113460.1"
                     /translation="MSSPHPSLANPRDHDFDTRLRAAKSGAEWAWAAIYRDLAGPVTG
                     YLANRGAAEPDDLTSETFLQIARNISTFEGNESAFRSWVFVIAHRRLIDSRRARQRRP
                     ETITLADPTGGGEGGDVEEEALNRLTTVELQAAFEKLSESQSDVLALRIIGQLTLEET
                     ASVLGKRVGAVKAAQRRGLLALSQHLDLDGVTR"
     gene            1066..1581
                     /locus_tag="VFU96_09090"
     CDS             1066..1581
                     /locus_tag="VFU96_09090"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEU5113461.1"
                     /translation="MLFNEGRANDRGASLVEFAVVAPLLFLLLFGVIEFARLGHGFTT
                     VWTAAREGARYATTVGDNDSDGLPNYLDCASITEAALDKVIGMSLATSNVAITFTDLT
                     DTPVADCDGATPLPAPSPSGSTDIDNGFGVQVEVEGTFNAIVPLLSSFLDGIDLTSRQ
                     NRSVFKGVVGE"
     gene            1574..2026
                     /locus_tag="VFU96_09095"
     CDS             1574..2026
                     /locus_tag="VFU96_09095"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEU5113462.1"
                     /translation="MSRQRSSDRGAAAVEMAFVSMFLLVLLLGIVDVGRAIVANISVR
                     DAVQDGASYFSYTKDATAADVEARVRSAVSSPDLSTAATTVAVYCGPDPRDLLDGSRI
                     RIDVTHDVDMITPIVGPFLGGEITLSPSSEADRFFDTCPADALAFPGP"
     gene            2023..3261
                     /locus_tag="VFU96_09100"
     CDS             2023..3261
                     /locus_tag="VFU96_09100"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEU5113463.1"
                     /translation="MTINRDSHTEKGAAAILVAISLFVLVGFAALAIDGGLGFDQRRG
                     TQNAADNAALAAAWEACNPRDASAPDPVGSALAVAAENGYDDTDPEVVVTVNALDATE
                     YEVIIQRVNDTTFAGPGVGAETLTVISQGVATCEQEPFLGGYAIFAGADACASGGAIE
                     LDLTGATKTINGGIFSNGDLKINGPNTTINGAVEYRGGFTSNAGVPGEKYFGSPKPYP
                     LDLDIAEFRPGGSRQASDPSHYYFSAPSIGIDNGWMVSNGHATGNNSNIVIIHSGIYY
                     SPKTGNSAIDLKGVSTAPGVTVTFVSEGEMHVIGEADITGYAPVDAGGYSPLILFSNA
                     SPTPSCSTNAIQFSGSDMSWAGLIYAPNGQVQMSASSNNATVNGSIIGYSVNVSGSNF
                     EINWQDDTSGEPRFIVELQS"
BASE COUNT          624 a         1144 c         1025 g          630 t
ORIGIN      
        1 cgcatgctga gacccagccg accgcccact cggcgacgct ggagcttcag gagctcgctg
       61 gcttcgggtc gggtcgccct caacacagag gaggcgccga cggcgaaccg ctgcacggcg
      121 gattcggatg gcctgcccac ccattgtgtt cggaccatct ccacgaccgg gacaagaccc
      181 gacagccgtc cgcctgcgac aggcgtgccg gcgagcagcc gctcgatctc ccaatccagg
      241 accttggtgg tcgccatctc gcccatgtaa tcgtcgggga atctcatcgg gttaccccgt
      301 ccaaatcgag atgctggctc aaggcgagga ggccccgtcg ctgtgcagcc ttcaccgcgc
      361 cgactcgctt gcccaagacc gaagcggttt cctcgagcgt cagctgcccg attatccgga
      421 gagcgaggac atcggattgg ctctccgaca gcttctcgaa agcggcttgc agttccacgg
      481 tggtcaagcg attcagcgct tcctcctcca catcccctcc ttccccaccc ccggtggggt
      541 cggccagagt gatggtttcg gggcgacgtt gtcgcgcccg tcgtgagtcg atgaggcgcc
      601 ggtgggctat cacgaagacc caggagcgaa aggccgactc gttcccctcg aatgtcgaaa
      661 tgttcctggc aatctgcagg aaggtctccg acgtcaggtc gtctggctcc gccgcacccc
      721 gattggccag ataaccggta acaggccctg cgaggtctcg atagatcgca gcccaggccc
      781 attccgcccc gctcttggcg gcgcgaagcc tcgtgtcgaa atcgtgatca cgagggttgg
      841 cgaggctggg atggggcgag gacatgggtt gcttgattgt ggaccggtcg attccactta
      901 agggctgctc cggttgcaga atgatatgtc ccttttgtat cgacagtccc ggctctgttc
      961 tgagcaatca tcgctcaacc gggtaacttt tgagtaaccc tctgtcgatc gcggacgata
     1021 acgaacccga agagtcgcgg attgaatccg tgaggggggt gctcggtgct gttcaacgag
     1081 ggtagggcga atgatcgtgg cgcgagcctg gtcgaattcg ccgtggtcgc gcccctcctc
     1141 tttctcctcc tcttcggggt catcgagttt gcccggctgg gacacggctt caccacggtc
     1201 tggactgcgg cccgagaggg ggcgcgttac gccaccacgg tcggtgacaa cgacagcgat
     1261 ggcctgccca actacctcga ttgcgcctcg atcaccgagg ccgcccttga caaggtcatc
     1321 ggcatgagcc tcgccaccag caacgtcgcc atcaccttca ccgatctcac cgacaccccc
     1381 gtggccgatt gcgacggtgc gacccctctg ccggcgcctt cgccatcggg gtccaccgac
     1441 atcgacaatg gcttcggcgt ccaggtcgaa gtggagggca ccttcaacgc catcgttccc
     1501 ctcctctcgt ccttcctgga cgggatcgac ctcacgtcga ggcagaaccg ttcggtcttc
     1561 aaaggagtcg tcggtgagta ggcaacgctc ttctgatcgg ggggctgccg ccgtcgagat
     1621 ggcctttgtg agcatgttcc tcctcgtcct cctcctggga attgtcgacg tcggcagggc
     1681 catcgtcgcc aacatctccg tccgtgacgc agtccaggac ggagccagct atttctccta
     1741 caccaaggac gccactgccg ccgacgtcga ggcaagagtc cggtcagctg tctcctcacc
     1801 cgatttgagc acggccgcga ccaccgttgc cgtctactgc gggcccgacc cgcgtgacct
     1861 cctcgacgga tcacggatac ggatcgatgt cacccacgac gtcgacatga tcaccccgat
     1921 cgtggggccc tttctcggcg gagagatcac cctcagcccg agttccgagg cagatcgatt
     1981 cttcgacacc tgccctgccg acgcattggc atttcccgga ccatgaccat caacagagac
     2041 agccataccg aaaaaggtgc cgccgccatc ttggtggcga tctccctctt cgtgctggtg
     2101 ggtttcgccg ccctggccat cgacgggggt ctcggattcg accagcgacg ggggacgcag
     2161 aacgcagccg acaacgccgc gctggccgcg gcctgggagg catgcaaccc ccgcgacgcc
     2221 tccgcgcccg acccggtggg gtcggccctc gccgtcgccg ccgagaatgg ctacgacgac
     2281 accgatcccg aggtcgtcgt cacggtcaat gccctcgacg ccaccgagta cgaggtgatc
     2341 atccagaggg tcaacgacac cacttttgcc gggcccggcg tcggggcgga gacgctgaca
     2401 gtgatctcgc aaggcgtggc cacctgcgaa caggagccgt tcctgggggg ttacgccatc
     2461 ttcgccggtg ccgatgcctg cgcttccggt ggagccatcg agctcgacct gaccggggcc
     2521 accaagacga tcaacggtgg gatcttctcc aacggcgacc tgaagatcaa cggccccaac
     2581 acgaccatca acggcgccgt cgagtatcgg ggaggattca cctccaacgc aggggtcccg
     2641 ggagagaagt atttcggctc tcccaagccc tacccactcg atctcgacat cgccgaattc
     2701 cgacccggcg ggtcccgtca ggcatcagat cccagccact attacttctc agcacccagc
     2761 atcggcatcg acaacggctg gatggtttcc aacggccatg ccaccgggaa caactccaac
     2821 atcgtgatca tccattcggg gatctactac tcacccaaga ccgggaacag cgcgatagac
     2881 ctcaagggcg tgagcacggc acctggcgtg acggtcacat tcgtctctga gggagagatg
     2941 catgtgatcg gcgaggccga tatcaccggg tacgcaccgg tcgacgccgg tgggtattcg
     3001 cccctgatcc tgttctccaa cgcctcgccg accccgagtt gcagcaccaa tgccatccag
     3061 ttcagtggga gcgacatgag ctgggcaggt ctcatctatg cccccaatgg tcaggtgcag
     3121 atgtctgcct cctcgaacaa tgccaccgtc aacgggagca tcatcggcta ttcggtcaac
     3181 gtgtcgggtt ccaacttcga gatcaactgg caggacgaca catccgggga gccccggttc
     3241 atcgtcgagc tccagagctg atcgccaggg tcagcgggtc caatacagga aggcgacttg
     3301 ctgggcgggg agggccgtcc ccgagaagac cgtgacctca tcgagtgagg cgagaaaggg
     3361 ccggttcgcc gtgatcgtcg agtcggtgcc cgggtctttg ttgccgcccc attggtcgcc
     3421 cca
//
LOCUS       DASTXO010000433         3891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_726078, whole genome shotgun sequence.
ACCESSION   DASTXO010000433 DASTXO010000000
VERSION     DASTXO010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726078"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..1474)
                     /locus_tag="VFU96_09105"
     CDS             complement(200..1474)
                     /locus_tag="VFU96_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5113464.1"
                     /translation="MGIESRASPQTPKTRRPKIHYGWWVVVASGAAVLLAAGIRSAPG
                     VFLLPIENDLALDRSTVSLAVSLGLLVYGLSAPFSGKLIDNIGSRAVATIGLIVIAGS
                     MLLSALSQNAMQLTLFFGILSGVGTGLLGSVLGATIANRWFVRNRGLVIGIFGATTSA
                     GQLLFLPLLATMAAGIGWRQTSVWLAAGAALLVIPVAIFVRDDPSDLGLPPYGGTTPV
                     DPPKPEPRIMRRAVRSIDFWLLVGTFGICGLTSNGIVGTHFITHAVEHGFTATVAAGT
                     LAVMGAFNFVGTLASGWLTDRYDPRRLLLVYYGFRGVSLFLVPVIHDRMGLMAFAVLF
                     GLDYIATVPPTVALAADSFGRQNVGTVYGWIFASHQLGAAVAAWAGGFVRQETGSYGP
                     AFLVAGSMAVLAGMAALAIRASRGRGLGEPAI"
     gene            complement(1495..2205)
                     /locus_tag="VFU96_09110"
     CDS             complement(1495..2205)
                     /locus_tag="VFU96_09110"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5113465.1"
                     /translation="MDTKTLESRISDLTAALGDPTRRAIYVAVRESAEPLTTSAVAEL
                     FGIHPNVARHHLDRLADDGYLKVSHRRPSGKGGPGAGRPAKCYEATTKEVSVHFAPRR
                     FEMLVEMLIRVLDQVAPENVGEVAESVGRAYGAELAAEIGVPDEPGYDGAVQAVARAM
                     TGLGFSVDPDVQGQRLLTSHCPFGEAATSHPEVICSLDRGIVAGMFGALSYDCNPVII
                     PHTDLEEDCVTRVPVTIG"
     gene            2275..3357
                     /locus_tag="VFU96_09115"
     CDS             2275..3357
                     /locus_tag="VFU96_09115"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEU5113466.1"
                     /translation="MPSNDEIFEAVASIESPFLVGRTLGSLGLVKSVEKKIGGRVKVD
                     LLLPTANPPETLNDRLQQALQPYGRGADVTVEVMTEQTAIEWIGKLREEAGPGLGEPG
                     SPTRVLAISSGKGGVGKSSVSSNLAVSVRRGGARVGIVDGDIWGFSIPRMLGVTQPPL
                     LVGEAIVPPTAHGVKVMSMDYFVGDDKAVIWRGPMLHKAIEQFLKDVFWDALEFLIVD
                     MPPGTGDIAISMSQFLPRAQVLLVTTPQPTAQRVARRAALMAAEVDQQVIGVVENMSW
                     FTGDDGKRYELFGSGGGASLAAELGVDLMAQIPLLPAMGRGADEGHPVSIAAPGSEAA
                     EAFDHLAKSVVDKRPRIRTNPALVIN"
     gene            3354..>3891
                     /locus_tag="VFU96_09120"
     CDS             3354..>3891
                     /locus_tag="VFU96_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113467.1"
                     /translation="MSSREQSRRFAVGVAGWVFGLASTVLLVGIWGRAVVVDTGELAD
                     TLSPLAAGDMVSDRLATWLEAELVEAGVDGVGASLAADQVLAHPSVGPVLEQLVAGGV
                     EAASSSAPTGASVDVAAILLPATGQITTGLNEAGVPVSKEQVEAALSQLDPLVIRDPS
                     DQPFVGASSPLAANLGTAA"
BASE COUNT          700 a         1252 c         1321 g          618 t
ORIGIN      
        1 gaggttctca acgccttcga tcgaatcgag gcgggctatc tcccgggacc cgagcggagt
       61 ttcgagcgtt gcgcgggacc cgaatggatc ggtgggcatg agccaaggtt accggcggac
      121 cgggaatccc aagcatgggt ctggccgcga aaagccggcc ggtcttggcg ttatcggaag
      181 gattccgcgt cgctcagcct cagatcgcgg gttctccgag accgcgaccc cgtgaggcgc
      241 ggatggcgag cgcggccatg ccggccagga ccgccattga cccggcaacg aggaaggcgg
      301 ggccgtagct ccccgtttcc tggcgcacga agcctccagc ccatgcagcg accgccgcgc
      361 ccagttgatg agaggcgaag atccacccgt acaccgtgcc gacgttctgg cgcccgaagg
      421 aatcggcggc gagcgccacg gtgggtggga ccgtggcgat gtagtcgagc ccgaacaaga
      481 cggcgaaggc catcagcccc atccgatcgt ggatgaccgg cacgagaaag agcgacacgc
      541 cgcggaaccc gtaatacacc agcaggagcc gccgggggtc gtagcgatcg gtgagccagc
      601 ccgaggccag ggtgcccacg aagttgaacg cccccatcac ggccagtgtt ccagccgcga
      661 ccgtcgctgt gaatccgtgc tccacggcat gggtgatgaa gtgggtgccg acaatgccgt
      721 tggaggtcag tccgcagatg ccgaaggttc cgaccagcaa ccagaagtcg atgcttcgaa
      781 cggcccgtcg catgattctc ggctccggct tgggcggatc gaccggcgtc gtgcccccgt
      841 acggcggaag ccccagatcg gagggatcgt cgcgcacgaa aatggcgacc ggtatcacca
      901 gcagggcggc gcctgcggcc agccaaacag atgtctgacg ccagccgatt cctgcagcca
      961 tcgtggcgag caggggcaag aagagcagtt gtccggccga ggtagtcgcc ccgaagatcc
     1021 cgatcacgag gccccggttg cggacaaacc atcggttggc gatcgtggcc ccgagcaccg
     1081 agccgaggag acctgtaccc acgccactga ggatcccgaa gaagagcgtg agctgcatcg
     1141 cgttctggga gagggcgctg agcagcatcg acccggcgat gacgatcaga ccgatggtgg
     1201 ccacggcccg agatccgatg ttgtcgatca attttcccga aaacggagcc gagagcccgt
     1261 atacgaggag gcccaacgac actgccagtg acaccgtgga ccggtccaac gccaggtcgt
     1321 tctcgatggg aagcaggaat acgccgggag cggatcggat ccccgccgcg agcaggacgg
     1381 cggcaccgga ggcgaccacc acccaccagc cgtagtggat cttggggcga cgggtcttcg
     1441 gcgtctgggg ggacgccctg gattcgatgc ccacgattcg gttaccgcgc tcgactatcc
     1501 gatggtcacc gggacccggg tcacgcagtc ttcctcgaga tcggtgtgag ggatgatcac
     1561 cgggttgcag tcgtaagaaa gggcgccgaa catcccggcg acgatgcccc ggtccagcga
     1621 gcagatgacc tcggggtgac tcgtggcagc ctcgccgaac gggcagtggc tggtgagcag
     1681 gcgctggccc tggacatcgg ggtcgaccga gaacccgagc ccggtcatcg ccctggccac
     1741 ggcctggacc gccccgtcgt atccgggctc atccgggaca ccgatctcgg cggccaattc
     1801 ggcgccgtag gcacggccca ccgattcggc cacctcaccg acgttctcgg gcgcaacctg
     1861 gtcgaggacc ctgatcaaca tctccacgag catctcgaac cgtcgcggcg cgaaatggac
     1921 cgagacctcc ttggtggtcg cctcgtagca cttggccggc cgaccggctc cgggcccgcc
     1981 cttgccggat ggacgacgat ggctgacctt caggtaacca tcatcggcga gacggtcgag
     2041 atggtgacgg gcgacattcg gatggatgcc gaacaactcg gcgacagcag aagtggtcag
     2101 tggttccgcc gattccctga ccgcgacgta gatggcccga cgggtgggat cacccaaggc
     2161 cgcagtcagg tcggagatcc tcgattcgag ggtcttggtg tccatgaggg gtaccaaccg
     2221 gccaaaacta tagtgaccag ggccttctaa ccactgccgg cgtaggggaa atcgatgcct
     2281 tccaacgacg agatcttcga ggccgtcgcg tccatcgagt ctccgttcct ggtcggtagg
     2341 acccttggca gcctcggcct cgtcaagtcg gtcgagaaga agattggggg ccgggtgaag
     2401 gtcgacctcc tcctccctac cgccaatcca ccggagacac tgaacgaccg tctccagcag
     2461 gcgctgcagc cctacggtcg cggagcggac gtgaccgtgg aggtcatgac cgagcagacc
     2521 gcgatcgagt ggatcggcaa gttacgcgag gaggcgggtc cggggctcgg cgagcccggc
     2581 tccccgacga gggtgctcgc catatcctca ggcaagggag gagtcggcaa gtcgtccgtc
     2641 agctccaacc tcgcggtctc ggtgcgacga ggcggggccc gtgtgggaat tgtcgacggc
     2701 gacatctggg gcttctccat cccccgcatg ctcggagtga cccagccccc gctcctggtg
     2761 ggcgaggcga tcgtccctcc cacggcccat ggcgtgaagg tgatgtcgat ggactatttc
     2821 gtcggtgacg acaaagcggt gatatggcgc ggcccgatgc tgcacaaggc gatcgaacaa
     2881 ttcctcaagg acgtcttctg ggacgcgctc gaattcctga tcgtcgacat gccgcccggc
     2941 accggtgaca tcgccatctc gatgtcccag ttcctccccc gtgctcaggt gctcctcgtc
     3001 accacgcccc agccgaccgc tcagagggtg gcccggcgag ccgccttgat ggcggcggaa
     3061 gtcgaccagc aggtgatcgg cgtggtggag aacatgtcct ggttcaccgg tgacgacggc
     3121 aagcgttatg agctgttcgg aagcggtggc ggtgcgtcgc tggccgcaga gctgggggtt
     3181 gacctgatgg ctcagatccc cctcctgccg gcgatgggtc ggggcgccga cgaaggccac
     3241 ccggtctcta tcgcggcccc cggatccgag gcggcagagg cgttcgacca tctggccaag
     3301 tccgtcgtcg acaagcggcc ccgcatcaga accaacccgg ctctggtgat caattgagct
     3361 cccgtgagca gagccggcgg ttcgccgtgg gtgtggcggg ttgggtgttc ggactcgctt
     3421 ctactgtgct cctggtcggg atatgggggc gggctgtcgt cgtcgacacc ggcgagctgg
     3481 ccgacaccct gagcccgctg gcggcaggag acatggtttc cgatcgcctg gctacctggc
     3541 tggaggccga gctagtcgaa gccggggtcg acggggtcgg cgcctcgtta gcggccgatc
     3601 aggtgctggc ccatccttca gtcggaccag tcctggaaca gctggtcgcc ggaggcgtcg
     3661 aagccgcatc ttcgtccgca ccaaccgggg cctcggtcga tgtggcagcc atcctgctcc
     3721 cggcaactgg tcagatcacc acgggcctga acgaggcggg cgtgccggtg agcaaagaac
     3781 aagtcgaggc cgccctgtcc cagctcgacc cactcgtgat ccgcgacccg tcggatcagc
     3841 cttttgtcgg cgcgtcctcc ccgctggcgg ccaatctcgg cacggctgcg g
//
LOCUS       DASTXO010000434         2931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_504859, whole genome shotgun sequence.
ACCESSION   DASTXO010000434 DASTXO010000000
VERSION     DASTXO010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504859"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VFU96_09125"
     CDS             complement(<1..549)
                     /locus_tag="VFU96_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HEU5113468.1"
                     /translation="MLATPILRAEELKKHFPVTRGLLWTKVIGWVQAVDGISFSIPQG
                     ETLALVGESGCGKTTTAKLILRLERPTSGQVFVDGKDVHVLNGDALKEYRTMVQAVFQ
                     DPWSSLSPRMRVQQIVAEPLVVNRQVSSEEVKERVAEILAAVGLRPQQANLYPHEFSG
                     GQRQRIAVASALVSSPKLIILDE"
     gene            complement(543..1532)
                     /locus_tag="VFU96_09130"
     CDS             complement(543..1532)
                     /locus_tag="VFU96_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5113469.1"
                     /translation="MARQTGDVILEVEDLHTYCFTRWGVVKAVDGVSFSLRQGEALGI
                     VGESGCGKTMTALSLLRLVPRPAARIVKGAIRLEGENLLDKSEREMRSIRGRRISMIL
                     QDPQTSLNPVFTVGNQLIEAIKIHHRDGHRSLVQRAIDSLKQVRLAAPERRVQDYPHQ
                     MSGGMKQRVVGAIAISCEPKVIIADEPTTSLDVTIQAQYLRLLREIQEETNLALIFIT
                     HDFGIVAKMCDRVMVMYAGRVVESGSVRDIFNHPSHPYTQALLNSVPSMDEDVERLYS
                     ITGQPPALWDLPAGCRFAARCPYADDRCRKDYPPLFTVGEGHTAGCWRLEQAC"
     gene            complement(1613..>2931)
                     /locus_tag="VFU96_09135"
     CDS             complement(1613..>2931)
                     /locus_tag="VFU96_09135"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5113470.1"
                     /translation="LRKGVPFHFGFGQFTARDVVHSHSMMMRPEATATLAGFWRQVEE
                     VKAVNDHQVVFRFKQPTTVMDYGASRAGDLRIVSKAQSDKEGIDGFDKRPAGTGSYRY
                     AGRQSGLSISFERVDNHWKGEKPAFRQLEFRVVREESTRLAMLLSGEAHIADLPRELQ
                     KDALQRGMKIYSSSFPVDWMTIYLGGMYFMPGDKEFKAEVPWTNKKVRQAMNMAVNRK
                     ELLNTVFAGKGTYTYVTGWLPESEGWNPEWQRRFDGMYGYNPSKAKALLKEAGYGPGQ
                     LKMKILAFTEPGESEGPQVAEALGIYFKDVGIETEIEVLDWAKVREMFRKKTIGCCIW
                     PNIISWRPSEEHVRTFNYSKGPNHHYENEFIDKTYLAWAQAVKPEERQRHARALADHF
                     FEEFADIPLFWFANEVVVNPKIVGDWVYPGLAAGRSTHFHLVQPAK"
BASE COUNT          514 a          927 c          891 g          599 t
ORIGIN      
        1 ctcgtctaag atgatgagct tcggactcga taccagggcg ctggccacgg cgatgcgctg
       61 gcgttgcccg ccgctgaact cgtgggggta gaggttggcc tgctgcggac gcaggccgac
      121 ggcggccaga atctcggcga cgcgctcctt aacctcttca gaggacacct gccggtttac
      181 caccagtggc tcggccacga tctgctgcac ccgcatgcgg ggacttagcg acgaccaggg
      241 atcctgaaag accgcctgaa ccatcgtccg gtactctttc agagcgtcgc cgttcaacac
      301 gtggacgtct tttccatcga cgaacacctg gcctgaggtc gggcgctcca gacgcaggat
      361 gagcttagca gtcgtcgtct tgccgcagcc agactcgccc accagggcga gcgtctcccc
      421 ctgtggaatg gagaagctga tgccatccac ggcttgaacc cagccgatga cttttgtcca
      481 gagtaggccc cgggtgacgg gaaagtgttt tttcagctcc tcggcccgga ggatgggcgt
      541 ggctaacatg cctgttccaa tctccagcaa ccggcggtgt ggccctctcc tacggtgaac
      601 agcggaggat agtctttccg gcaacggtca tcggcatagg ggcagcgagc ggcaaaccgg
      661 cagcccgcgg gaaggtccca gagcgcgggc ggctggccgg tgatcgaata aagccgctcg
      721 acgtcctcgt ccatgctggg gacggagttc aacaaggctt gcgtgtacgg atgggacgga
      781 tgattgaaga tgtcccgcac cgagccgctt tccaccaccc ggccggcgta catcaccatg
      841 acgcggtcgc acatcttcgc cacgatgccg aagtcgtgag tgatgaagat cagcgcgagg
      901 ttcgtctcct cttgaatctc gcggagcagg cgcaggtatt gagcctggat cgtcacgtcc
      961 agtgaagtgg tgggctcgtc ggcgatgatg actttcggct cgcaggagat ggcgatcgcg
     1021 ccgaccacgc gctgcttcat accgccgctc atctggtgcg ggtaatcctg tacccggcgc
     1081 tcgggagccg cgagccgcac ttgcttcaag ctgtcgatcg cccgctgcac cagagatcgg
     1141 tgaccgtctc tgtggtggat tttgatggct tcgatcagct ggttgccgac cgtgaacacc
     1201 ggattgaggg aggtctgcgg gtcctggaga atcatcgaga tgcgtcgccc gcgtatgctt
     1261 cgcatctcac gctcgctctt gtcgaggaga ttctcccctt cgaggcggat ggctcccttg
     1321 acgatccgcg ctgccggccg ggggaccaat ctcaataacg acagggcggt catggtcttc
     1381 ccgcagcccg actcgcccac gatgcccaga gcttctccct gtcgtaagga gaagctcacg
     1441 ccatccacgg ccttcacgac gccccagcga gtgaagcagt aggtgtggag gtcctcgact
     1501 tccaggatga cgtcgccggt ctgtctggcc atgcactacc taccgtcgtc ggatatggca
     1561 gggcatcaag aggaagattg tcttcccctt gatgccttgt gcctcgctgg tcctacttcg
     1621 cgggttgcac gagatggaag tgcgtcgacc gcccggcggc gagccccggg tagacccagt
     1681 cccccacgat cttggggttc acgacgacct cgttcgcgaa ccaaaagagc gggatgtcgg
     1741 cgaactcctc gaagaaatgg tcggcgagcg cccgggcatg gcgctgccgt tcctcgggct
     1801 tcacggcctg ggcccaggcg aggtaggtct tgtcgataaa ctcgttctcg taatggtggt
     1861 tcggtccctt gctgtaatta aacgtccgga catgctcctc cgacggcctc caggagatga
     1921 tgttgggcca gatgcagcag ccgatcgtct tcttgcggaa catctcgcgc accttggccc
     1981 agtcgagcac ctcgatctcc gtctcgatgc ccacgtcctt gaaatagatc cccagggcct
     2041 cggcgacctg cggcccttcc gactccccgg gctcggtgaa cgccaggatc ttcattttga
     2101 gctggcctgg cccgtagccg gcctccttca gaagcgcctt ggccttggag gggttgtagc
     2161 catacatccc gtcgaatcgc ctctgccact ccggattcca gccctctgat tcggggagcc
     2221 agccggtcac gtacgtgtag gttcccttgc cggcgaacac cgtgttgagg agctccttgc
     2281 ggttaacggc catgttcatg gcctgcctga ccttcttgtt ggtccagggc acctcggcct
     2341 taaactcctt gtcccccggc atgaaataca tacctcccag atagatcgtc atccagtcca
     2401 cggggaatga cgacgaatag atcttcatac ccctctgtag cgcgtccttc tgcagctcgc
     2461 ggggcaggtc ggcgatgtgg gcctctcccg acagcagcat cgccagccgt gtcgactcct
     2521 ctcggaccac tctgaactct agctgcctaa atgccggctt ttcgcctttc cagtggttgt
     2581 cgacccgctc gaagctgatg gacaaacctg attggcgccc ggcatagcgg taggagccgg
     2641 ttccggccgg ccgcttgtca aacccgtcga ttccttcctt gtccgactga gccttgctga
     2701 cgatcctgag atcgccggcg cgcgaggcac cgtaatccat gacggtcgtc ggctgcttga
     2761 aacggaacac gacctggtgg tcgttgacgg ccttcacctc ctcgacctgg cgccagaacc
     2821 cggccagggt cgcggtcgct tccggacgca tcatcatgga gtgggaatgg acgacgtctc
     2881 tggcggtgaa ctggccgaat ccgaaatgga agggcacgcc cttgcgcagg a
//
LOCUS       DASTXO010000435         2850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_836218, whole genome shotgun sequence.
ACCESSION   DASTXO010000435 DASTXO010000000
VERSION     DASTXO010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836218"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="VFU96_09140"
     CDS             <1..937
                     /locus_tag="VFU96_09140"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU5113471.1"
                     /translation="RFAETHGIKKTLLDRALTANRKRVLAEQDGASGPMLAGALDSIV
                     LSKVREQLGMDQVEIAITAAAPINPDLILFFQTLGIPLFELYGMSETTGPGTTNVPGA
                     FRLGSVGKALPGVEVELGEDDELLMRGGVITQGYYKLPGDTAEAFDAQGWLHSGDLAR
                     IDDDGFVWIVGRKKEIIITAGGKNIAPAKLETILGNHPLISKACMIGDGRKFLAMIVA
                     LDHEEAPAWAEANGLVYQDLATFSQLPEVNSEIERAIDEANEIVSSVEQVKKWIIVPD
                     EWTPDSGEVTPSLKLKRKVVLDKYANEIESLYVGV"
     gene            957..2105
                     /locus_tag="VFU96_09145"
     CDS             957..2105
                     /locus_tag="VFU96_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5113472.1"
                     /translation="MDRDLFEADHELFRSTAREFVEREVAPYVAKWEAQGKVDKDMFR
                     KAGKAGLLGMALPEEHGGGGIADFRFNMVIGEELMRADAMASGMCITLHNDVCLPYFT
                     HLCDVGQAARWMPGLVSGDLMSAIAMTEPGAGSDLAGIKTTAIRDGEVYVVNGSKTFI
                     TNGINSDLVITAVKTDPNERHGGMSLLVIEEGMDGFSRGRNLEKIGLHAQDTAELFFD
                     DVRVPVSNRLGDEGRGFVYLMENLPQERLSLATGSVAHAQAVFDWTLDYVRERQAFGQ
                     PIGSFQTVKHVMAEMRTELDVSQTYVDRQVIALNRGELSAEEAAKAKWWVTEMEKRVI
                     DRCLQLFGGWGYMEEYPIARAYRDARVQTIYGGTTEIMKEIIGRSLGL"
     gene            2150..>2850
                     /locus_tag="VFU96_09150"
     CDS             2150..>2850
                     /locus_tag="VFU96_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5113473.1"
                     /translation="MGEPITFDGRVAVVTGAGGGLGKEYALELGRRGAMVVVNDLGGA
                     VDGSGSSHSAADQVVDQIKGDGGESVASYDSVATREGGEGIIRTALDAFGSVDIVINN
                     AGILRDKSFANMTLDEVDAVLNVHLRGAFHVSHPAFKVMKEQGYGRFVHTTSASGLFG
                     NFGQANYGAAKAGLVGLSNVLAIEGAKYNIKSNAVAPTARTRMTEDILGPFVEMLDPR
                     QVMPMVVYLCSEQNEL"
BASE COUNT          578 a          818 c          954 g          500 t
ORIGIN      
        1 ccgtttcgca gagactcatg gaatcaagaa gacattgctc gaccgggccc tcaccgccaa
       61 ccggaaacgt gtcctggccg aacaggatgg ggcatcgggc ccgatgttgg cgggtgcgct
      121 cgactcgatc gtcctctcca aggttcgcga acagctgggg atggaccagg tcgagatcgc
      181 gatcacggct gcggcaccga tcaacccgga cctgatcctt ttctttcaga cgctgggcat
      241 ccccctcttc gagttgtacg ggatgtcgga gaccaccggc ccgggcacga ccaacgttcc
      301 cggagctttc cgcctcggct cggtggggaa agccctgccc ggtgtcgagg tggagctcgg
      361 agaggacgac gaactgttga tgaggggagg ggtcataacc cagggttatt acaagctccc
      421 cggtgacacc gccgaggcat tcgatgccca gggctggctt cactccggcg acctcgcccg
      481 catcgacgac gatggattcg tgtggatcgt cggtcgtaag aaggagatca tcatcacagc
      541 cggtggcaag aacatcgccc cggccaagct ggagacgatc ctgggcaacc atcccctgat
      601 ctcgaaggca tgcatgatcg gcgatggccg caagttcctg gcgatgatcg tcgccctcga
      661 tcacgaagag gcgccggcgt gggccgaggc caacggcctc gtctaccagg atctggccac
      721 cttctcccag ctccccgagg tgaattcgga gatagagcgg gccatcgacg aggcgaatga
      781 gattgtctcc tcggtcgagc aggtgaagaa atggatcatc gtccccgacg agtggacgcc
      841 agactcgggt gaggtgacgc cgtctctgaa gctcaagcgc aaagtcgtgc tcgacaagta
      901 cgccaatgag atcgagagcc tctacgtagg agtctgagac ccggccccgg ggggacatgg
      961 atagagacct gttcgaagcg gaccacgagc tgtttcgctc cacggcccgt gagttcgtcg
     1021 agcgagaggt tgctccctac gtcgccaaat gggaggcgca gggcaaggtc gacaaagaca
     1081 tgtttcgcaa ggcgggcaag gccgggctcc tcggcatggc attgcccgag gagcatggcg
     1141 gcggcgggat agcggacttc cgcttcaaca tggtgatagg cgaggaattg atgcgcgccg
     1201 atgccatggc ctccgggatg tgcatcaccc tccacaacga tgtctgtctt ccatatttca
     1261 cccatctatg cgacgtgggg caggcggcgc ggtggatgcc cggcttggtg tcgggggacc
     1321 tgatgtcggc catagcgatg accgagcccg gagccggctc ggatctcgcc gggatcaaga
     1381 cgacggccat tcgcgacggc gaggtatatg tcgtcaacgg gtccaagacg ttcatcacga
     1441 atgggatcaa ttccgacctt gttatcactg cggtcaagac cgaccccaac gagagacacg
     1501 gcggtatgag cctcctcgtc atcgaggagg gcatggatgg attcagtcga ggcagaaacc
     1561 tggagaagat cgggctccat gcacaggaca ccgctgagct cttcttcgac gatgtcaggg
     1621 ttccggtctc gaatcggttg ggggatgaag ggaggggatt cgtctacctg atggagaacc
     1681 tgccccagga gcggctcagt ctcgccaccg gctctgtcgc ccacgcccag gctgtgttcg
     1741 actggaccct cgattacgtc cgagaacgtc aggcatttgg tcagcccatc gggagctttc
     1801 agacggtgaa gcacgtcatg gcggagatga ggaccgagct cgacgtctcc cagacgtatg
     1861 tcgaccgcca ggtcattgcg ctgaaccggg gcgagttgag cgctgaggag gcggccaagg
     1921 cgaaatggtg ggtgaccgag atggagaagc gggtgatcga ccgctgtctc cagctctttg
     1981 gaggatgggg atacatggag gaatacccca tcgcccgggc ataccgggac gctcgtgtcc
     2041 agaccatcta cgggggcacg accgagatca tgaaggagat catcgggcgg tccctcggtc
     2101 tgtagtggcg ggccaccaac caggaatcaa atcggaggga gtcaaacaaa tgggtgaacc
     2161 aatcacgttc gatgggcggg tggcagtggt caccggggcc ggtggcgggc tcggaaagga
     2221 gtacgcgctc gagctgggga gaagaggggc gatggtcgtg gtcaacgacc tgggtggcgc
     2281 agtcgacggg agtggctcgt cccattccgc cgccgaccag gtggtggatc agatcaaggg
     2341 agacggcggc gagtccgtgg ccagctacga ctccgtcgcc actcgggagg gtggtgaggg
     2401 catcatccgg accgccctgg acgcgttcgg atcggtcgac atcgtgatca acaacgccgg
     2461 gatcctcaga gacaagtcat tcgccaacat gaccctcgac gaggtggacg cagtcctcaa
     2521 tgtgcaccta aggggcgcct tccacgtctc acacccggca ttcaaggtga tgaaggagca
     2581 ggggtacggt cgattcgtcc acaccacgtc ggcctcgggg ctgttcggga acttcggtca
     2641 ggccaattac ggcgcggcga aggccggcct ggtcggtctg tccaacgtcc tcgccatcga
     2701 gggggcgaag tacaacatca agtcgaatgc ggtggcgcct acggcccgca cccggatgac
     2761 cgaggacatc ctcggtccct tcgtggagat gctcgacccg aggcaggtga tgccgatggt
     2821 ggtctatctc tgctcggagc agaacgagct
//
LOCUS       DASTXO010000436         9303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1720681, whole genome shotgun sequence.
ACCESSION   DASTXO010000436 DASTXO010000000
VERSION     DASTXO010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 9303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9303
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1720681"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /gene="nhaA"
                     /locus_tag="VFU96_09155"
     CDS             <1..1131
                     /gene="nhaA"
                     /locus_tag="VFU96_09155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEU5113474.1"
                     /translation="DETVLHLINDGLMAIFFFVVGLEIKRELVLGDLRDRRTATLPVV
                     AALGGMVLPALIYIMITFDPGGEALRGWGIPMATDIAFAVGVVALLGPRVPSGAKLFL
                     LTLAIADDIGAITVIAIFYTEEVSLTYLALAIGGLALVRIATTVGIRSLAFYVPLGIL
                     IWFLTLESGVHATLAGVALGFLTPARPMYPAAEFDRRARAILDSFSDEPDSSESREHA
                     DHEALLLSDISSEAVSPLSRLEYRLQTWSSFVVIPVFALANAGVDFRGMDIGRALTSD
                     VALGVAVGLVVGKILGVSLFSFIAVKAGLGRLPEQTTWRHIVGLGAVAGIGFTVALFV
                     TSLAFRDPTLIDLAKVGIYAGSLIAGVLGFALLSRAHGTSTR"
     gene            1128..1316
                     /locus_tag="VFU96_09160"
     CDS             1128..1316
                     /locus_tag="VFU96_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113475.1"
                     /translation="MTRPPENFTCVDCGGVAHLISFLPEDSDPEPGIALAYRCADCLE
                     RFDVVWEEDVDDGEAPSP"
     gene            1392..2006
                     /locus_tag="VFU96_09165"
     CDS             1392..2006
                     /locus_tag="VFU96_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020663829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU5113476.1"
                     /translation="MNTRHRAEPLAPDDRRRAIVEAVIPLLVERGATVTTRQMAEAAG
                     IAEGTIFRVFPDKSALIHEAVRVSIDPGPVQKDLAEIYADAPLETQLAEAARILLERF
                     DIVITLLGVLRTLPHFGIEGKTPGPPAFVTVANGAINQSLAQIFDRHRDRLKIDPARA
                     ASAFRGLLLANAHPTMELTERLTIDEIVSVLLTGIVTPSLERMS"
     gene            2006..3739
                     /locus_tag="VFU96_09170"
     CDS             2006..3739
                     /locus_tag="VFU96_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018786108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5113477.1"
                     /translation="MLIRIVKEYLQPYRRWLTIIVALQLVGTIASLYLPSLNADIIDQ
                     GVVTGDTDYIIRVGAVMLGISLLQIACTIVAVYFGARTAMGFGRDLRSKIFHRVGEFS
                     SREMAKFGAPSLITRNTNDVMQVQTLVFMSLTMMLMAPIMMVGGIVMALREDVGLFWL
                     VAVAVPVLGVAIGFIVSHMVPGYRLMQGRIDIVNRVLREQTSGIRVLRAFVREPFETE
                     RFGRANSELTDVSLSVGRWMAAMFPVLMLVLNVSSVAVLWFGAMRIEAGAMEIGALTA
                     FLSYLIQILWSVMMATFMLVMIPRAAVSSDRIAEVLDTESSVVLPISGVNEVDRRGEL
                     ELTGVGFMYPGATHPVLDDVSFVTRPGQITAIIGSTGAGKTTLLTLIPRLIDATEGRV
                     LVDGVDVRDFDAETLWSKIGLVPQQAYLFSGTVASNLRYGKPDATEEEMWTALEIAQA
                     RDFVAAMDGGLEAPIAQGGTNVSGGQRQRLAIARALIRRPEIYLFDDSFSALDLATDA
                     RLRRALKPYTADSTMVIVAQRVSTIADADQILVLEDGAVVGLGTHESLLATCPTYVEI
                     VESQLRADVAS"
     gene            3736..5712
                     /locus_tag="VFU96_09175"
     CDS             3736..5712
                     /locus_tag="VFU96_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5113478.1"
                     /translation="MSDRTMKTTERVQAGPGPGRGPFGGGMIGQKSLEFGPSAKRLLA
                     RLRPERPKVFAVLLTAVLSVGLSAIGPRVLGWATDIIFAGASGSRLPAGMTKAEAIDA
                     ARATGQGQVADLLSAVDLVPGQGIDFRALATVLLFALAIYVGSALLGYLQGYLLNDVV
                     QKTIFRMRSEVEDKLNRLPLRYFDRQPRGELLSRVTNDIDNVSQSLQQTMSQMLNSLL
                     TVIFVVGMMFSISPTLALIALVTIPLTMVIAGTVMKRSQKHFISQWRRTGSLNAQIEE
                     AFTGHSLVKVFGRQREVQESFEVENEALYESSFSAQFLSGLIMPIMMFIGNLNYVIVA
                     VVGGLRVASGTMTLGDVQAFIQYSRQFAQPITQIASMINLLQSGVASAERVFELLDAD
                     EEVADDDQPMMVDDPHGQVAFEHVSFRYDEDRPLIEDLSLTVEPGQTVAIVGPTGAGK
                     TTLVNLIMRFYELDSGKITLDGVDIADMRRADLRSEIGMVLQDTWLFRGTIRENIAYG
                     RPDATEDEIMEAARATFVDRFVHSLPDGYDTMIDDEGSNVSAGEKQLITIARAFLADP
                     ALLILDEATSSVDTRTEALLQHAMAALRSDRTSFVIAHRLSTIRDADLILVMESGRIV
                     EQGNHDQLIALDGAYAALYNSQFSGADEEPEAAA"
     gene            complement(5716..6588)
                     /locus_tag="VFU96_09180"
     CDS             complement(5716..6588)
                     /locus_tag="VFU96_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015345950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEU5113479.1"
                     /translation="MRLHLLDGTYELFRSHYGRKDTVTGPDGADLKATLGIVESTLAL
                     LREPGVTHLAASFDTVIESFRNIMFPAYKSSAGMDPDLLAQFPVAETALEAIGVVVWR
                     NIEFEADDAIATAAIRWVEDVDQVVIATPDKDLNQLVIGERIVTYNRREQKLLDEAAV
                     VEKFGIHPESIPDYLALVGDTADGVPGLPGWGAKSAATVLSRYPRLELIPELAADWEV
                     TVRGGDKLAAVLRENQEAVHLFRELTTLRLDVPIDESLADLEWKGVPRASFESFCASV
                     GFDPGEIRVHRWAE"
     gene            6577..6924
                     /locus_tag="VFU96_09185"
     CDS             6577..6924
                     /locus_tag="VFU96_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113480.1"
                     /translation="METHRPDPTSNGETDPWDAASLEWGSLKDRLWSTYRQAASTGGP
                     TEEEIKKALATLAGAWDQVAASFSAALSDPDTRDSLKRAAGSFAAALGATVSGLGEDF
                     RRRDEANPPPSEE"
     gene            6929..7732
                     /locus_tag="VFU96_09190"
     CDS             6929..7732
                     /locus_tag="VFU96_09190"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEU5113481.1"
                     /translation="MPGPLIGVFELQQSLDEVKLFDLRWSLTSPTPGRESYETGHIPG
                     AVFVDLDRDLAGDQGDGRHPLPHISEFVDTLGRLGLEPEDDVVVYDDMSGTVAARMWW
                     MLESIGHPGRVRVLDGGLDEWVKRGLTLESGSVTPARAVYPEVAGFTGAVRHDDLEGR
                     FLADVRSPERYRGEVEPVDPKAGHVPGAVNYPVSGNLHDGRFRASSDLAEVYRDFPED
                     GVVSCGSGVNACHGALALVIAGKPMPDVYIGSFSDWSRRDLPVATGPNP"
     gene            7729..8688
                     /locus_tag="VFU96_09195"
     CDS             7729..8688
                     /locus_tag="VFU96_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113482.1"
                     /translation="MITARAIEAIESSDTDELLRVIDGLCASRSWDDLIELRHRCAEA
                     VGRGKQVWGIEEHIRYRLVLEAPAQIAGPVLAEGQSRFALGPLPEVAASTKTWVDLAP
                     HIPPGPERDTVAAERVVRGEPVDVSIPDLPAMLMTWEPAYPTAAYKKDKVEAPAPTIV
                     ETAAAVLPDEVDSIGDADSLAALGDLVEPWTDQSNGRSQAAAVNGDHLAAIAALGMTR
                     ARVAPIDPSHGLAWMGWAAASGGAHGRRRGAAAGRYLAWWAVATLIDLDWPADPESMG
                     KSVAGLSWHWFDDGSPETGWLLRLAISSATLGLSWAIAATDQV"
     gene            complement(8690..>9303)
                     /locus_tag="VFU96_09200"
     CDS             complement(8690..>9303)
                     /locus_tag="VFU96_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003937510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU5113483.1"
                     /translation="RADAEQLVGRSVDDFTVGFPVEAVYDGRDALIAVGMNGEPLPME
                     HGFPARLVVAGLYGYVSATKWLSSIELTGWDDFDAYWIPRGWAKEAPIKTQSRIDTPT
                     EGASMTPGVRQVAGVAWAPNRGISKVEVKLGEEAEWVEARLAEPLSDNCWRQWVVDWD
                     AQAGTHQLQVRATDGEGNVQTDQHRPPAPDGATGWHTIRVSVG"
BASE COUNT         1568 a         3072 c         2976 g         1687 t
ORIGIN      
        1 gacgagaccg tcctccatct gatcaacgac ggcctgatgg cgatcttctt cttcgtggtc
       61 ggtctcgaga tcaagcgaga gctggtactc ggtgacctga gggatcggag gacagcgact
      121 ctccccgtgg tggcggcatt ggggggaatg gtccttcccg ccttgatcta catcatgatc
      181 acgttcgatc ccggcgggga ggcgctgcga gggtggggga tccccatggc cacggacatc
      241 gctttcgcgg tgggagtggt cgcccttctc gggccacggg tgccgagcgg ggccaagttg
      301 ttcctcctca cactggccat cgccgacgac atcggggcca tcaccgtgat cgcgatcttc
      361 tacacagaag aggtgagcct cacctacctc gccctggcca ttgggggcct ggcgctggtt
      421 cggatcgcca ctacggtcgg tatccggtct ctggctttct atgtgccgct tgggatcctg
      481 atctggttcc tgaccctcga atctggagtc catgccacgt tggccggggt cgcactcgga
      541 tttctcaccc cggccaggcc gatgtacccg gcggccgaat tcgaccgtcg ggcacgggcc
      601 atcctcgaca gcttcagcga cgagccggac agctcggaga gccgtgagca cgccgatcac
      661 gaggcgctgc tgttgagcga tatctcgtcg gaagcggttt ctcctttgag ccggctcgag
      721 taccgcctcc agacgtggag ctcgttcgtt gtcatacccg tgttcgcgct ggccaacgcc
      781 ggcgtcgatt tccgagggat ggacatcggc cgcgccctga cctccgatgt cgccctgggg
      841 gtggcggtcg ggctggtcgt cggcaagatc ttgggggtga gcctgttcag cttcatcgcg
      901 gtgaaggcag ggctcggtcg cctccccgaa cagaccacct ggcgccacat agttggcctg
      961 ggggcggtgg ccggcatcgg gttcacggtg gcgctgttcg tcaccagcct cgccttccgg
     1021 gacccgaccc tcatcgacct ggccaaagtc ggcatctacg ccgggtcgct gatcgccggt
     1081 gtcctcggat tcgcattgtt gtctcgggct cacggcacca gcaccagatg accagacctc
     1141 cagagaactt cacttgcgtc gactgtggtg gggtcgctca cctcatctcg tttctccccg
     1201 aggacagcga tcctgagccg ggcatcgctc tcgcctaccg ttgcgccgac tgtctggagc
     1261 gcttcgacgt cgtctgggag gaggacgtcg acgatggcga ggccccttcc ccttgagtct
     1321 cacgggcccg atcctggtca ggcgctggtt ccgatttgtg agtgagcgct cactcactta
     1381 aacttcgccg cgtgaacacg agacataggg ccgagccgct ggctcccgac gaccgacgac
     1441 gggccatcgt cgaagcggtc atccctctcc tcgtcgagcg cggggcgacg gtgacgacgc
     1501 gtcaaatggc cgaagccgcc ggcatcgccg agggcaccat cttccgggtc ttccccgaca
     1561 agagcgccct catccacgag gcggttcggg tgagtatcga tcccggtccg gtccagaagg
     1621 atctggcgga gatctatgca gatgctccgc tggagacgca gttggccgag gcagcccgaa
     1681 tcctcctcga gcgattcgac atcgtcatca cactgctggg cgtcctccgc accctccccc
     1741 atttcgggat cgagggcaag acgccagggc cacctgcgtt cgtgaccgtc gccaacggcg
     1801 cgatcaatca gtcgctcgcc cagatcttcg atcgacaccg ggatcggctg aaaatcgacc
     1861 cggcacgggc cgcctctgcg ttccgggggc ttctgctcgc caatgcccac ccgaccatgg
     1921 agctcacaga acggctgacg atcgatgaga tagtcagcgt cttgctcacc ggcatcgtga
     1981 ctccttccct ggagaggatg agctgatgct gattcgaatc gtcaaggagt atctgcagcc
     2041 ctacaggaga tggctgacga tcatcgtcgc gctccaactg gtcggcacca tcgcctcgct
     2101 gtatctcccc agcttgaacg cagacatcat cgaccagggt gtcgtgaccg gggacaccga
     2161 ttacatcatt cgggttggag cggtgatgct cggcatctcg ctcctccaga tcgcctgcac
     2221 gatcgtcgcc gtctacttcg gcgccaggac cgccatgggc ttcgggcgtg acctccgttc
     2281 caagatcttc caccgagtcg gcgagttctc cagccgcgag atggccaagt tcggcgcacc
     2341 ctcgctgatc acccgcaaca cgaacgatgt catgcaggtg cagaccctcg tcttcatgag
     2401 cctgaccatg atgttgatgg ccccgatcat gatggtgggc gggatcgtca tggccctgcg
     2461 cgaagacgtc gggttgttct ggctggtggc ggtggctgtg ccggtcctcg gagtggcgat
     2521 cggcttcatc gtgtcccaca tggtgccggg atatcgcctg atgcaagggc ggatcgacat
     2581 cgtgaaccgg gtcctccgcg agcagacatc cggcatccgg gtcctccgcg cattcgttcg
     2641 cgagccgttc gaaaccgaac ggtttggccg tgccaacagc gagctgaccg atgtctcgct
     2701 gtcggtgggc agatggatgg ccgcgatgtt cccggtgttg atgctggtcc tcaatgtgtc
     2761 aagcgtggcc gtcctctggt tcggcgcgat gcggatcgaa gccggcgcga tggagatcgg
     2821 ggccctcacc gcattcctct cctatctgat ccagatcctc tggtccgtga tgatggccac
     2881 cttcatgctg gtcatgatcc ctcgggcggc ggtcagctcc gaccgcatcg ccgaggtgct
     2941 cgacaccgaa tcatcggtgg tgctgcccat ctccggcgtg aacgaggtcg atcgtcgcgg
     3001 ggagctggag ctgaccggtg tcggtttcat gtatccgggc gcaacccatc ccgttctcga
     3061 cgatgtcagc ttcgtgactc gacccggcca gatcaccgcg atcatcgggt ccaccggtgc
     3121 cggcaaaacg actctgctca ccctcatccc caggctgatc gacgccaccg agggaagggt
     3181 gctcgtcgat ggcgtggacg tccgtgactt cgacgccgag acgctgtggt cgaagatcgg
     3241 tctggttccc cagcaggcat acctcttctc cgggacggtg gccagcaacc tccgatacgg
     3301 gaagcccgac gcgaccgaag aggagatgtg gaccgccctc gagatcgccc aagcccgtga
     3361 tttcgtggct gcgatggatg ggggtctcga agcgccgatc gcacagggag gcaccaacgt
     3421 gtccggaggt cagcgtcagc ggctcgcgat cgcccgcgcc ttgatccggc gtccggagat
     3481 ctatctcttc gacgattcct tctccgctct cgacctggca acagacgccc gtctgcgtcg
     3541 cgccctgaag ccctacaccg ccgactccac gatggtgatc gtggctcagc gtgtgtccac
     3601 catcgccgac gccgaccaga tcctggttct ggaagacggg gcggtcgtcg ggctcgggac
     3661 ccatgagtcc ctgttggcga cctgcccgac atatgtggag atcgtcgaat cgcaactgag
     3721 ggcggacgtg gcctcatgag cgatcggacg atgaagacca ccgagcgagt ccaggccggg
     3781 cccggacccg ggcgtgggcc gttcggcggc gggatgatcg gtcagaagtc gctcgaattc
     3841 gggccttcgg caaaaaggct gcttgcccgg ctccgccccg agcgccccaa agtcttcgcc
     3901 gtgctcctca ccgcggttct cagcgttggc ctgtccgcga tcgggcccag ggtcctgggc
     3961 tgggccaccg acatcatttt cgccggcgcc agcggcagcc ggctcccggc cgggatgacc
     4021 aaggccgaag cgatcgacgc cgcacgcgcc accggtcagg gtcaggtcgc cgacctgctg
     4081 tccgcggtgg acctggtccc gggtcagggc atcgactttc gcgcattggc gacggtgctc
     4141 ctctttgccc tcgccatcta cgtcggctcg gccctgctcg gctacctcca gggctatctg
     4201 ctcaacgacg tggtccagaa aaccatcttc aggatgcgat ccgaagtcga ggacaagctc
     4261 aaccggcttc cgctccgtta cttcgaccgc cagccgcgcg gtgagttgtt gagccgggtc
     4321 accaatgaca tcgacaacgt gtcccagagc ctccagcaga cgatgagcca gatgttgaac
     4381 tccctcctca ccgtgatctt cgtggtcggg atgatgttct ccatatcacc gactctggcg
     4441 ctgatcgcac tggtgacgat cccgctcacc atggtcattg ccggcacggt gatgaagagg
     4501 tcacagaagc atttcatatc gcaatggcgt cgcaccgggt ccctcaacgc acagatcgag
     4561 gaggcgttca caggtcacag cctggtcaag gtcttcggcc gtcagcgcga ggtgcaggag
     4621 agcttcgagg tggagaacga ggctctgtac gaatccagtt tctccgcaca gttcctctcc
     4681 ggcttgatca tgccgatcat gatgttcatt gggaacctga actacgtgat cgtggctgtg
     4741 gtcggcggac tgcgggtggc cagcggcacc atgaccctcg gagacgtcca ggcgttcatc
     4801 cagtactcac gccagttcgc tcaacccatc acccagatcg cctccatgat caacctcctg
     4861 cagtccggcg tcgcttcggc ggagcgcgtg ttcgagctgc tcgatgccga cgaggaggtc
     4921 gccgacgacg accagccgat gatggtcgac gacccgcacg gacaggtcgc cttcgagcac
     4981 gtgtcgttcc gatacgacga ggatcggcca ctcatcgagg atctctctct gacggtcgag
     5041 cccgggcaaa ccgtggccat cgtcggtccc accggcgccg gcaagacgac gctcgtgaac
     5101 ctgatcatgc ggttctacga gctggactcg gggaagatca ctctcgacgg agtcgacatc
     5161 gcagacatgc ggcgcgccga cctccgctcc gagatcggga tggtgttgca ggacacctgg
     5221 ctgttccggg ggacgatccg tgagaacatc gcctacggcc gccccgatgc gaccgaggac
     5281 gagatcatgg aggcagcgcg tgcgacattc gtcgatcggt tcgtgcacag ccttcccgac
     5341 ggttacgaca cgatgatcga cgacgaggga agcaacgtca gcgccggtga gaaacagctc
     5401 atcaccatcg cccgtgcgtt cctcgccgac ccggctctct tgatcctgga tgaggctacg
     5461 agctcggtcg acacccgcac cgaggccctg ctccagcatg cgatggccgc gcttcgctcc
     5521 gaccgcacca gcttcgtcat tgcccaccgg ctctccacca tccgcgacgc cgatctcatc
     5581 ctcgtgatgg agtcaggacg catcgtcgag caaggcaacc acgaccagct gatcgccctg
     5641 gacggcgcct acgccgccct ctacaactca cagttctcgg gggcggatga ggagcccgag
     5701 gccgcggcct aggacctact cggcccagcg gtggacccgg atctccccag ggtcgaagcc
     5761 gacggaggcg cagaaagatt cgaaggacgc acgtggaacg cccttccact cgaggtcggc
     5821 cagcgactcg tcgatcggca cgtcgagccg gagcgtcgtc agctcgcgaa agagatgcac
     5881 ggcttcctgg ttctccctga gcacggcggc gagcttgtcc cctccgcgca ccgtgacctc
     5941 ccagtcggca gccaattcgg ggatcagctc gagacgggga tagcgagaga gcacggtcgc
     6001 ggccgatttc gcgccccacc cggggagacc cggcacgccg tcggcggtgt caccgaccag
     6061 agccaggtag tcgggtatgg actcgggatg gatcccgaac ttctccacaa cggcggcctc
     6121 gtcgagcagt ttctgctctc gtcggttgta ggtgacgatc cgctcgccga tgacgagctg
     6181 gttcagatcc ttgtcggggg tcgcgatgac cacctggtcc acgtcctcga cccatcggat
     6241 cgcggctgtc gcgatggcgt cgtccgcctc gaactcgata tttcgccaaa cgacgactcc
     6301 gatcgcttcc agagccgtct cagcgaccgg gaactgggcg aggaggtcgg gatccatccc
     6361 cgcggatgac ttgtaggcag ggaacatgat gttgcggaac gactcgatca ccgtgtcgaa
     6421 gctggcagcc aggtgggtga ccccaggctc acgaaggagg gccagcgttg attcgacgat
     6481 tccgagcgtg gccttgagat cggccccatc gggaccggtc accgtgtcct tgcggccgta
     6541 gtgggagcga aacagctcgt aggtaccatc gagaaggtgg agacgcaccg gcccgatcct
     6601 acgtcgaacg gggagacaga tccctgggat gccgcctccc tggaatgggg ctccttgaag
     6661 gatcgcctct ggagcaccta ccggcaggcc gcctccaccg gtggcccgac cgaggaagag
     6721 atcaagaagg cacttgccac cctggccggc gcctgggacc aggtcgcagc ctccttctcc
     6781 gcagcgctga gcgaccccga cactcgggac agcctgaaac gggccgccgg ctccttcgct
     6841 gccgccctcg gcgccaccgt ctccggcctc ggtgaggact tcagacgccg ggacgaggca
     6901 aatcctccac catccgagga gtgagctggt gcccggcccg ctgatcggcg tcttcgagct
     6961 gcaacagagc ctggatgaag tgaagctgtt cgatctgcgc tggtcgctca cgtcgcccac
     7021 cccgggtcgc gagtcctacg agacggggca cattccgggc gccgtcttcg tcgacctcga
     7081 ccgcgatctg gccggagatc agggtgacgg gcggcatccg ctcccccata tctccgaatt
     7141 cgtagacacc ctcgggcgtc tcggtctcga gcccgaggac gacgtcgtcg tgtatgacga
     7201 catgtcggga acggtcgcag cccggatgtg gtggatgctg gaatcgatcg gccatccggg
     7261 ccgggtgagg gtgctcgacg gagggctcga cgaatgggtt aagcggggcc tgaccctgga
     7321 gagcggctcg gtgaccccgg cgcgagctgt ctatcccgag gtggccgggt tcaccggcgc
     7381 ggttcgacac gacgacttgg aggggagatt cctggccgac gtcagatcgc ccgagagata
     7441 tcggggcgag gtcgagccgg tggaccccaa ggccgggcac gttcctgggg cggtgaacta
     7501 tcccgtctcg ggcaatctcc atgatggacg cttccgggcg agcagcgacc tcgcagaggt
     7561 ctatcgggac ttcccggagg atggggtggt ttcctgtggc agtggggtga acgcgtgcca
     7621 tggcgccctg gccctcgtaa tcgccgggaa gcccatgccc gacgtctaca tcgggtcgtt
     7681 ctcggactgg tcgagacgtg atctgccggt tgcaaccggg cccaatccgt gatcacggca
     7741 cgggccatcg aagcgatcga atcgtctgac accgacgagt tgctccgggt catcgacggg
     7801 ctctgcgcca gccgaagctg ggacgacctc atcgagctcc ggcatcgatg cgccgaagcg
     7861 gtgggaaggg ggaaacaggt ctggggcatc gaggagcaca ttcgatatcg cctcgtgctc
     7921 gaggccccgg cacagatcgc cggaccggtg ctggctgagg gccaatctcg ctttgcccta
     7981 ggtccccttc ccgaagtggc ggcgagcacc aagacgtggg tcgacctggc cccgcatatc
     8041 ccaccggggc cggaacggga cacggtcgcc gccgagcggg tggtccgtgg ggaaccggtc
     8101 gacgtgtcga tccccgacct ccccgcgatg ctgatgacat gggagccggc gtaccccacc
     8161 gcggcataca agaaggacaa ggtcgaggca cccgctccga ccatcgtcga gaccgcggcg
     8221 gccgttctgc ccgatgaggt ggattcgatc ggcgacgccg actcactggc cgctcttggt
     8281 gacctcgtcg agccctggac cgatcagtcg aacgggcgct cgcaggcggc cgcagtgaac
     8341 ggagatcacc tggcggcaat cgcggcgctg ggaatgacgc gagcgcgggt tgccccgatt
     8401 gatccgagcc acggtctggc ctggatggga tgggctgccg cgtccggagg ggcgcacggc
     8461 cgccgtcgtg gcgctgccgc cgggcgctat ctggcctggt gggcggtggc gacgctcatc
     8521 gacctggact ggccggcaga ccccgaatcg atgggcaagt ctgtcgccgg cctcagctgg
     8581 cattggttcg acgatgggag cccggagacc ggctggctgc tccgtttggc catctccagc
     8641 gccaccctcg gtctgtcctg ggccatcgcc gccacagacc aggtttgact caacccacac
     8701 tgacccgaat cgtgtgccag cccgtggcac cgtccggagc cggggggcga tgctggtcgg
     8761 tctggacatt gccttcgcca tcggtggcgc gcacctggag ctggtgggtc ccggcctgcg
     8821 cgtcccaatc gaccacccat tgccgccagc aattgtcgga caatggctcg gcgagccgcg
     8881 cctccaccca ctcggcctcc tcgcccaatt tcacctcgac cttcgagatt ccccgattcg
     8941 gtgcccaggc cactccagcg acctggcgca cgcccggcgt catcgacgca ccctcagtcg
     9001 gggtgtcgat gcggctctgg gtcttgatcg gtgcctcctt cgcccaaccc cgcggaatcc
     9061 agtaggcgtc gaaatcgtcc catccggtca gctcgatcga cgaaagccat ttggtcgcgg
     9121 agacgtagcc gtagagaccc gccactacga gacgggccgg aaacccgtgc tccatcggca
     9181 atggctcgcc attcatgccc accgcgatca gggcatcccg accgtcgtag acggcctcaa
     9241 ccgggaatcc gaccgtgaaa tcgtccaccg accgaccaac cagctgctcg gcgtccgcgc
     9301 gga
//
LOCUS       DASTXO010000437         5115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_284232, whole genome shotgun sequence.
ACCESSION   DASTXO010000437 DASTXO010000000
VERSION     DASTXO010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5115
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284232"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VFU96_09205"
     CDS             <1..200
                     /locus_tag="VFU96_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113484.1"
                     /translation="TEILVLGENLEESPYGIRASGGGGSDPARGDYLAVVFDIPEPGC
                     WRISAVYRATGIDYVVWVDGD"
     gene            complement(234..1259)
                     /locus_tag="VFU96_09210"
     CDS             complement(234..1259)
                     /locus_tag="VFU96_09210"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEU5113485.1"
                     /translation="MTVRVGVLGAGTVGGALVNRLLADRDAIRLKSGIDLELRRVAVR
                     DLSKERPFDIKDGRMTDDALSVVTDPSIDVIVEVIGGLEPAGALVLAALRSGKPVVTA
                     NKELIATRGHEITEAAKRAGVNVMYEAAVGGGIPVIRPLAESLAGEDIDVVSGIVNGT
                     TNFILTAMSKEGRGYDDALAEAQRLGYAESDPADDVSGRDAAFKTSILASLAFGERVA
                     PWEVECEGIEGVTSADVASATDRNEVIKLIGSAQRGIDGRVEASVKPVSLPADHPLAG
                     VNGVTNAIFIEGPSVGRLLFTGPGAGGQPTASAMLGDLIQVASRGRGPRTGSAHDPKP
                     RRQVLHR"
     regulatory      1569..1655
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1771..>5115
                     /gene="metH"
                     /locus_tag="VFU96_09215"
     CDS             1771..>5115
                     /gene="metH"
                     /locus_tag="VFU96_09215"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEU5113486.1"
                     /translation="MTDYLEQVRSGLVIFDGATGTNLQTVGLTAEDFGGPSLEGCNEL
                     LNLRRPDVIRDLHRTFLEVGVDVVETNTFGAFAVPLGEYGLEEQSYEIAASGATLARE
                     IVDEFIKADARPRYVAGSIGPGTKFATLGQISYRDLAAAYEVESSGLIDGGADLLIIE
                     TQFDLLGAKAAIEGARRAMRAAGVEIPLQVQVTIELTGRMLPGTEIGAALTTLEALGP
                     DVIGLNCATGPAEMYEPLRHLARHSTVPVSAIPNAGLPHVEDGQMAYDLSPDDLAHHL
                     GQFVTEMGIGVIGGCCGTSPDHLAAVVERTRPLPLHKRNPQTVPSAASIYSQVPFDQD
                     TSFLIIGERANANGSRAFKEALLDDDLDGCVSVGQDQVKEGAHLVDLCVDYVGRDGVA
                     DMVKLAAAFATDVAAPVVLDSTEPEVMEAGLERLGGRSLLNSANLEDGEEPGSRLDRV
                     FGLARKHGAGAICLLIDEEGQARTLEWKMRIAHRIHDLAVERYGLRSEDLIFDALTFP
                     LTTGDDDLRKDAMITIDAIRQIKTELPGVRTVLGVSNVSFGLNPAARRVLNSVFLHEA
                     VEAGLDAAIVHAGKIEPLNRIDPEQRQVCLDLIYDRRAEGYDPLEKLLELFAGVETVE
                     RSESDYEGMEVGERLAKRIIDGDQKNVITDLDVALGEGTEPLDIINDYLLDGMRTVGE
                     LFASGEMQLPFVLKSAETMKKAVAYLEPMMEKAEGSTRGRIVLATVSGDVHDIGKNLV
                     DIILTNNGYEVHNLGIKVGISEMIAKFEETSADAIGMSGLLVKSTLIMRDNLEELERR
                     GLGKVPVLLGGAALTRNYVEADLRSRYQGPLYYGKNAVAGLDAMGKIVAGEPAPEPIA
                     ARQYHERETEGGVIPARSPDVKRDNQIFVPPFTGSRVIRGVSIDEIVPYLNLTALFRN
                     QWQYRPEKGERDLDFKERLAPLLREQIALARHYDLLNPKVVYGYFPANGDGDDLVIWD
                     DVSAQRELTRFSFPRQREDPYLCIADFFRPVGEEVDYAAFHIVTMGDKVSDRTKDLFE
                     QDRYTDYLLLHGLGVEMAEALAEHWHHRIREEWGFVDEDGPSLLGLFRQQYRGGRFSW
                     GYPACPDLEDNEKVAYLLGADRIGIKV"
BASE COUNT          934 a         1628 c         1625 g          928 t
ORIGIN      
        1 gaaccgagat cctcgtactc ggtgagaatc tcgaggagtc gccttacggg attcgtgcaa
       61 gtggtggggg cggctctgac ccggctcgcg gtgactacct ggccgtcgtc ttcgacatcc
      121 ccgaaccggg gtgttggaga atcagcgccg tctaccgcgc cactggcatc gactacgtgg
      181 tttgggtgga cggcgattga gatctcaatc gttctgacct gcgagcagga acctcagcgg
      241 tggagcactt gacgtcgcgg cttggggtcg tgagccgatc cggtcctcgg gccacggcca
      301 cgcgaggcga cctggatcaa atcgccgagc atcgcgctcg cagttggttg accacctgcg
      361 ccgggaccgg tgaagaggag acggcccacc gaaggcccct cgatgaagat ggcgttggtc
      421 acaccgttga ctcccgccaa cgggtggtcg gcaggaaggc tcaccggttt cacacttgcc
      481 tcgacccggc catcgatccc gcgctgcgcc gagccgatga gcttgatgac ttcgttcctg
      541 tcggtagccg atgcgacgtc cgcggaggtc actccttcga tcccctcaca ttcgacttcc
      601 cacggcgcca cacgctcacc aaaggcgagg ctggcgagga tcgatgtctt gaacgcggca
      661 tcgcggcctg atacgtcatc ggccggatcg gattccgcat atccgagccg ctgggcctcg
      721 gcgagcgcgt cgtcgtagcc ccggccctcc ttggacatgg cggtgaggat gaagttggtg
      781 gttccattca cgatcccact caccacatcg atgtcctcgc cggcgaggct ctcggcgaga
      841 gggcgaatca ccgggatccc gccgccgacc gctgcctcgt acatgacgtt gaccccggcc
      901 cgctttgccg cctccgtgat ctcgtggccc cgggtcgcga tcagctcttt gttggcggtc
      961 accacgggct tcccagaccg cagcgcagcc aggaccaagg cgccggcggg ctcgaggccg
     1021 ccgatcacct cgacgatcac gtctatcgat ggatcggtca ccactgacaa cgcatcgtcc
     1081 gtcatccgac cgtctttgat gtcgaacgga cgttccttgg acaggtctcg caccgcgaca
     1141 cgcctgagct cgagatcgat ccccgacttg agcctgatgg catcacggtc ggccaagagg
     1201 cgattcacca gcgcaccgcc tacggtcccg gcaccaagca cgccaacccg aacggtcatg
     1261 gcctctgcct cgcttctctt tgctgtctca tggaccttcg ttttctccga tggccgccgc
     1321 cggggtgggg aatcaaaaag atccgcccct ctgccggtgc ggatctcgtg tctcgtgtgg
     1381 ttgtggcgct ggttacacgg acgagtcgca cggcctctcg gtcgtggtcg tcataccggt
     1441 cgtgagccaa gcgctcatgt gatgagcaag gtatccggga tcgtggatca agtcaatgcg
     1501 ggcagccctt tctcgctcgg gatattgcac aaatccggcg gaatgcaata gccttgtcat
     1561 cgacgaagcg ctcatcttga gcggttgagg gatcaggccc gatgaaaccg cggcaaccgg
     1621 cgcaagcacg gtgccaatgc ctggatcgat gagttgccgg agccatcggc tcccggcgct
     1681 cttcaagcgt ttcgagaaga aacagaggag tggaccggtg agcgaggctc agaagccttt
     1741 ctgcgagtgc aagccccagc ggtgtcgatc gtgaccgact atctcgaaca ggtccggtcc
     1801 ggtctcgtca tcttcgacgg ggccaccggc accaacctcc agaccgtcgg gctcacggcc
     1861 gaagacttcg gcggcccgtc gctcgagggc tgcaacgagt tgctgaacct gcgtcggccc
     1921 gacgtgatca gggacctgca ccgcacgttc ctcgaagtcg gcgtagatgt ggtcgagacc
     1981 aacaccttcg gggctttcgc cgtccccttg ggggagtacg ggctggagga acagtcctac
     2041 gagatcgcgg cgtcaggggc gacgctggca cgagagatcg tggacgagtt catcaaggcg
     2101 gatgcacggc cccgttatgt cgccggatcg atcggtcccg gcaccaagtt cgccactctc
     2161 ggccagatct cctatcgaga tctggctgca gcctacgagg tggaatcttc tggcctcatc
     2221 gacggaggcg ccgacctcct catcatcgaa acccagttcg acctcctcgg ggcgaaagcc
     2281 gcgatcgagg gcgcacgaag ggcgatgagg gctgccggcg tagagatccc actccaggtc
     2341 caggtcacca tcgagctcac cgggcggatg cttcctggta ctgagatcgg cgcggccctg
     2401 accactctcg aggcgctggg acccgatgtg attggcctca actgtgccac cgggcccgct
     2461 gagatgtatg agccgttgcg tcacctggct cgccactcga cggtgccggt gtcggcgata
     2521 cccaacgccg ggctgcccca tgtcgaagac gggcaaatgg cctacgacct gtccccggac
     2581 gatttggccc atcacctggg tcagttcgtc accgagatgg gaatcggtgt gatcggtggc
     2641 tgttgtggca cgagcccgga tcatctggcc gccgtcgtgg agaggacccg ccctcttcct
     2701 ctccacaagc gcaatccaca gaccgtccca tcggcggcct cgatctacag ccaggtccca
     2761 ttcgaccagg acacatcgtt cttgatcatc ggggagcggg ccaatgccaa cgggtcgcgc
     2821 gccttcaagg aggccctgct cgacgacgac ctcgatgggt gcgtgtccgt cggccaggat
     2881 caggtgaagg agggagctca tctcgtcgac ctctgtgtcg actatgtcgg gcgggatgga
     2941 gtggccgaca tggtgaagct ggccgcggct ttcgccaccg acgtcgccgc ccccgtcgtg
     3001 ctcgactcca ccgagccgga ggtgatggag gctggtctgg agcggctcgg tggtcgcagt
     3061 ctgctcaaca gcgcgaatct ggaggacggt gaagagccgg gctcgcgtct cgaccgggta
     3121 ttcgggctgg ctcgcaagca cggggccgga gccatatgcc tgctcatcga tgaggaggga
     3181 caggcgcgca ccctggagtg gaagatgcgg atcgcacatc gcatccacga cctcgccgtc
     3241 gagcgttacg ggctgcgttc agaggacctc atcttcgatg ccctcacctt tcccttgact
     3301 accggggacg acgatctgcg caaggacgcg atgatcacga tcgacgccat ccggcagatc
     3361 aagaccgagc tgcccggggt gagaacggtt ctcggtgtgt ccaatgtctc cttcggcctc
     3421 aacccggccg cacgacgcgt cctcaactcg gtgttcctcc acgaagctgt cgaggccggt
     3481 ctcgatgcgg cgattgtcca cgccgggaag atcgagccgc tcaaccgaat cgatcccgag
     3541 caacgccagg tctgtctcga cctcatctac gaccggcgcg ccgaggggta cgacccgttg
     3601 gagaagctcc tcgagttgtt cgccggggtc gagacggtgg agcgctcgga gagcgattat
     3661 gaaggcatgg aggtggggga gcggctcgcc aagcgcatca tcgacggcga ccagaagaat
     3721 gtcatcaccg atctcgacgt cgctttgggc gagggcaccg agcccctcga catcatcaac
     3781 gactacctcc tcgatgggat gaggacggtc ggcgagctct tcgccagcgg tgagatgcaa
     3841 ctcccgttcg ttctcaagtc ggccgagacg atgaaaaagg ccgtcgccta cctcgagccg
     3901 atgatggaga aagccgaggg gtcgacacgg ggccggatcg tgctcgccac cgtctcaggg
     3961 gacgtccacg acatcggcaa gaacctggtc gacatcatcc tcaccaacaa cggctacgag
     4021 gtgcacaacc tcggcatcaa ggtcggtatc tcggagatga tcgccaagtt cgaagagacg
     4081 tcagccgacg ccatcgggat gagtgggctc ttggtgaaga gcactttgat catgcgcgac
     4141 aacctcgagg agttggagcg acgtggtctg ggcaaggttc ccgtcctcct cggcggggcc
     4201 gccttgacca ggaactacgt cgaagccgat cttcgatccc gttatcaggg cccgctctac
     4261 tacggcaaga acgcggtcgc cgggctcgac gcaatgggaa agatcgtcgc cggcgagccc
     4321 gctccggagc cgatcgcggc ccggcagtat cacgaacggg agaccgaggg gggtgtgatc
     4381 cctgccaggt cgcccgacgt gaagcgggac aaccagatct tcgtgcctcc gttcaccggg
     4441 tctcgagtga tccggggagt ctccatcgac gagatcgtcc cctatctcaa cctgaccgca
     4501 cttttccgca accagtggca gtaccgaccc gagaaagggg agcgcgacct cgatttcaag
     4561 gagcggctgg cgccgctgct ccgggagcag atcgccctgg cccgccatta cgacctgctc
     4621 aaccccaagg tggtctatgg gtacttcccg gcgaacggtg acggcgacga cctcgtcatc
     4681 tgggacgacg tcagcgcaca acgggaattg acccgattca gcttcccgcg gcagcgggag
     4741 gatccgtatc tgtgcatcgc cgacttcttc cgaccggtgg gcgaagaagt cgactacgcc
     4801 gccttccaca tcgtcacgat gggcgacaag gtctccgacc gaaccaagga cttgttcgag
     4861 caagatcgct acacggatta cctgctcctt cacggtctcg gtgtggagat ggccgaggcc
     4921 ctcgccgagc actggcatca tcggatccgc gaagagtggg gtttcgtcga tgaggacggc
     4981 ccatctctcc tcgggctgtt ccgccagcaa tatcggggcg ggcggttctc atggggatac
     5041 ccggcatgtc cggacctcga ggacaacgag aaggtggcct atctgctcgg tgccgaccgc
     5101 atcgggatca aggtg
//
LOCUS       DASTXO010000438         2463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1167667, whole genome shotgun sequence.
ACCESSION   DASTXO010000438 DASTXO010000000
VERSION     DASTXO010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167667"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="VFU96_09220"
     CDS             <1..683
                     /locus_tag="VFU96_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113487.1"
                     /translation="DGRNRVRSPIALNAAAIEFAEEVSGSGAEGTVSIQVAFGYTGAY
                     TAGAHGLSPDVPVPGSVAMDPDQDFDPADPTGTTGHEIAVTDAAFLKIALDTADLTPA
                     NPAIDIDLYLYNSVGEEVAISTSGGTAEEILLEAPANDTYTLYVHGWQTTGITVAYNL
                     HTWLVPAAAGGGSLAINAAPASATQGTTGTVEAGWSGLGPGNYLGAVSHTGDSLLGYT
                     LVEVDNTP"
     gene            complement(726..1766)
                     /locus_tag="VFU96_09225"
     CDS             complement(726..1766)
                     /locus_tag="VFU96_09225"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEU5113488.1"
                     /translation="MTLEQRIRQQIEATGPIPFEVFMDLALYDPDDGFFTAGKLRSER
                     GGDFLTSPEVSPLFGETLSRYVEREQERIGPPFEVVEVAAGSGSLIRPLKAAIDVEVW
                     AVETSPAAREAVGSILDPDRVLSDLPDRIRGVVIANELLDNLPMAVAQMMNGGWRERW
                     VGVHEDRLAMVDAQPRVEVVDWLNAYAGEVPEGGWVEVQLAAGGWLGDVLNRLDAGSL
                     VIFDYGDTAENLIPRRQDGTLRTYRAHHLGPHPLDDPGETDITADVNFTALMAAAGQA
                     GVSVDLHRQDDFLDELGLGDRLSELRHAELEAARSGDEMERLRLRTLKTEAETLLHPR
                     GLGDFRVLVARK"
     gene            1784..>2463
                     /locus_tag="VFU96_09230"
     CDS             1784..>2463
                     /locus_tag="VFU96_09230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEU5113489.1"
                     /translation="MKFGVFMFPTDTAIRPDDLAREVEARGFDSLWIPEHSHIPSSRA
                     TPWGGVEGAPPLPDHYWRSHDAFIALAYAAAVTTRLRLGTGVTLVAQRDPIWTAKEVA
                     SLDSLSGGRVILGVGYGWNREEMAQHGVDYRRRRQLLREKVLLMKALWTEEEAAFTGE
                     LMELEPSWAWPKPTQRPHPPIIMGAAAGPKTIADMVEFCDGWMPLATRMDIVGEVGRV
                     REAVAATGR"
BASE COUNT          432 a          803 c          806 g          422 t
ORIGIN      
        1 tggacggtcg aaatcgggtg aggagcccga ttgcgctgaa cgccgccgcc atcgagttcg
       61 ccgaggaggt cagcggatcc ggtgccgaag gcacggtcag catccaagtc gctttcggat
      121 acaccggcgc ttataccgca ggagctcacg ggttgtctcc agatgttcct gtcccgggga
      181 gtgtcgcgat ggatcccgat caggatttcg acccggctga tccgacgggg accacaggtc
      241 acgagatcgc ggtgaccgac gcggcgttcc tcaagatcgc tctcgacacc gcggatctca
      301 cacctgccaa ccccgcaatc gatatcgacc tctacctgta caactcggta ggcgaagagg
      361 tggccatctc cacgtctggc gggacagcgg aggagatcct cctggaggca ccggccaacg
      421 acacctacac cctctatgtc catggctggc agacgaccgg gatcacggtg gcatacaacc
      481 tccatacctg gctggttccg gcggcggcgg gtggaggatc cctggcgatc aacgcggcgc
      541 cggcctcagc gacgcaaggc acgacaggga ccgtcgaggc cggatggtcc ggtcttggac
      601 ccgggaacta cctcggagct gtttcccaca ccggcgactc cctcctcggg tacaccctgg
      661 tcgaggtgga caacacgcct taggcgacac gggcagggct tcatgtttgt ggcgcccccg
      721 tagcgctact tgcgtgccac caggacccgg aagtcgccga gccctcgggg gtgcaagagc
      781 gtctcggcct ccgtcttcag cgtgcgcaga cgcaatcgct ccatctcgtc ccccgatctc
      841 gccgcctcca actcggcgtg gcggagttcg gataggcgat ccccgaggcc gagctcgtcg
      901 aggaagtcgt cctggcgatg gaggtcgaca ctcacaccgg cctgcccggc agctgccatc
      961 aatgcggtga agttgacgtc ggcggtgatg tcggtctccc ccgggtcgtc gagtggatgg
     1021 ggaccgagat gatgagcccg ataggtgcgg agtgtcccgt cctgacggcg ggggatcagg
     1081 ttctcggcgg tgtccccgta atcgaagatg acgagcgagc ctgcatccag gcggttgagg
     1141 acgtctccca gccagccccc ggccgcgagc tgcacctcga cccaaccacc ctctggcacc
     1201 tcgccggcgt atgcgttcag ccagtcgacg acctcgacgc gaggttgggc atcgaccatc
     1261 gccagccggt cttcgtggac tccaacccag cgctcccgcc agccgccgtt catcatttgc
     1321 gccactgcca tcggcagatt gtcgagaagc tcgttggcga tgacgacacc ccgaatccgg
     1381 tcgggcagat cgctcaacac tcgatcggga tcgaggatcg agcccaccgc ctctcgtgct
     1441 gccggcgatg tctcaacggc ccacacctcg acatcgatcg cagccttcag cgggcggatg
     1501 agagagcccg atccggcggc cacctcgacc acctcgaatg gcgggccgat ccgctcctgc
     1561 tcccgttcca cgtatcggga gagggtctca ccaaacagcg gactgacctc gggtgaagtg
     1621 aggaaatcgc ccccgcgctc ggagcggagc ttgccagcag tgaagaagcc atcatcgggg
     1681 tcgtagaggg caaggtccat gaagacctcg aacgggatcg ggccggtcgc ctcgatctgt
     1741 tgtcggattc tctgctcgag ggtcatcgcc tagcttctcg aagatgaaat tcggagtgtt
     1801 catgtttccc acggatacgg caattcgccc cgacgacctg gcccgagagg tcgaggcccg
     1861 gggcttcgac tcgttgtgga tcccggagca ctcccacatc ccatcgtcgc gggccacgcc
     1921 ttggggcggc gtagaggggg cgcccccact tccagaccat tactggcggt cgcacgacgc
     1981 gttcattgcc ctcgcctatg cggcagccgt caccaccagg cttcgattgg ggactggtgt
     2041 gaccctggtc gcccagcggg acccgatctg gacggccaag gaggtggcat cgctcgattc
     2101 gctgtccgga ggtcgtgtga tcctgggagt ggggtacggg tggaaccggg aggagatggc
     2161 ccagcatggc gtcgattaca ggcgtcgccg ccagttgctt cgggagaagg tgctgctgat
     2221 gaaggccctg tggaccgagg aggaggcagc ttttaccgga gagctcatgg aactcgagcc
     2281 ctcttgggcc tggccgaagc ccacacagcg gccccacccg ccaatcatca tgggtgccgc
     2341 cgccgggccc aagaccatcg ccgacatggt cgagttctgc gatgggtgga tgcccctggc
     2401 gacgaggatg gacatcgtcg gtgaggtggg gcgggttcgc gaagccgtgg ccgccaccgg
     2461 gcg
//
LOCUS       DASTXO010000439         4937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_284412, whole genome shotgun sequence.
ACCESSION   DASTXO010000439 DASTXO010000000
VERSION     DASTXO010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284412"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..452
                     /locus_tag="VFU96_09235"
     CDS             66..452
                     /locus_tag="VFU96_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEU5113490.1"
                     /translation="MLSHESRHLAEGANHAVLTTLFPSGRPQTNVVWIDHDGEHLLVN
                     SETERQKVRNVRRDPRVAVLVFDASDMDNWVEVRGQVVEIVEGEEARRHIDTLAQKYL
                     GVPDYPNPIRSPRVILKIRPDRENVY"
     gene            complement(457..1251)
                     /locus_tag="VFU96_09240"
     CDS             complement(457..1251)
                     /locus_tag="VFU96_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HEU5113491.1"
                     /translation="MTRVAVVGLGAVGRALVVALDRGLPGFELTAVSARDRESAEQLL
                     EGLGVDVPVVDVEQLISLADLVIECAPAKLFPRIAEPLLRAGKEIIVLSAGALLEHPE
                     LIELARAHGGVISVPTGALVGLDAIAAAAEGEISSLRLVTRKPPRGLAGAPGVEGIDL
                     DNLTEPVRVFSGTARDAAKGFPANLNVAAALALAGPGPDETQVEVWADPGVTKNTHTI
                     VMGSDVADLTLTIENIPSENPRTGRITAPSVISMLRKRTATLRVGS"
     gene            complement(1248..2138)
                     /locus_tag="VFU96_09245"
     CDS             complement(1248..2138)
                     /locus_tag="VFU96_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU5113492.1"
                     /translation="MLPTHGRFEYSPITSRPDFTWPGGRRLAVYVAVCIEHFSYGQGG
                     LGLSYSPGIPHPNTYNWAWREYGNRVGGWRLLEVFEQIGVTPTALINTSCYDHCPELI
                     DAYRSAETEFVAHGHTNSIHPNDLDEHEEGEMIRGIVKTMSAHEGKPPAGWMSPGANP
                     SAATEDLLAENGFVYTLDWPMDDQPTWMRTKGGPLLSVPYPHEVNDVPMVVFHHGTAP
                     AFADMMTDNLDEMVEQSRRQSLVFGIVAHTFIMGQPYRLRLFRAALEYLVGLPDIWLT
                     TPGEIASAYAGTFAPETALG"
     gene            2255..2686
                     /locus_tag="VFU96_09250"
     CDS             2255..2686
                     /locus_tag="VFU96_09250"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU5113493.1"
                     /translation="MVERTAITYPGHDAGLGGRIWSPGLKVGPWIFLSGITSVDYNEK
                     KTVGVEERTSMTPPKKDPEAQWRQVLSNIKAMMEEAGGTMADVVMANVFVTDIHYYYD
                     YEWVRKEFFVEPYPVCTAVEVGSLVHPDWMLEIEVIGYIEG"
     gene            2688..3056
                     /locus_tag="VFU96_09255"
     CDS             2688..3056
                     /locus_tag="VFU96_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113494.1"
                     /translation="MRGGGSDSYNLEDFASELEAAPVNRDVGTTLWFENDHVRVWEVR
                     LEPGQRAPFHAHTQTYFWTCIDPGKGFQRFPDGTAQAFDFQLGETVFSEITASDPMIH
                     DLENTDDQLVRFVAIELKDL"
     gene            3071..4357
                     /locus_tag="VFU96_09260"
     CDS             3071..4357
                     /locus_tag="VFU96_09260"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5113495.1"
                     /translation="MQQYDVVIVGGRVAGSTLASLLGAGGLEVLLLDRAEFPADTLST
                     HVIYGDSFGIWERIGAWGAIERLASQKLWGISWIRDGKPDIRGRFWPVRGHDYSLCLR
                     RLLLDDVLFKNAANTPGVTAIEAARVTDILLDGDTVNGVKFETSGSTTESTVAARLVV
                     GCDGRRSTIARAVGANQYVTEPPINFAFYTYIQDADTGPEPEPMFEIWDSEYNGGTPM
                     LAECDAAVWMAIVYLPQEHFDSFRADKDGNFWEAMDRDPRVGPRIRAGRQLTPIKGAG
                     DFVNFVRDPVGDGWALVGDAGQHKDPIFGQGIGDAVRSAEALADCLLEAEAASSDWGS
                     ALKRYRTKRDLDLIPNFQWMIQGKPADLTEGDFNLIMDSLGSDPDQAERFVNVFSHAV
                     SGSEFFGRVNASRLLGRDAATLDSRRMLTSSGKEGD"
     gene            4358..4828
                     /locus_tag="VFU96_09265"
     CDS             4358..4828
                     /locus_tag="VFU96_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU5113496.1"
                     /translation="MGAEDRITDLGYRLGESPPPLGQYVRCVVANGLAFTAGHGPFDE
                     SGKVSIRGQVGGDLSLEQGQEAARLAVVACLASLKAELGSLDEVERIVKMLAFVNCAP
                     GFVNTSGVIDGASDLLVDVFGPAGVHARAAIGTTVLPGGIPVEIELIVEVRDSS"
BASE COUNT          938 a         1523 c         1610 g          866 t
ORIGIN      
        1 gcacgttgaa acacggtttt gagaaagttt ccttcgacgg tgttgcggag cgataacgtt
       61 ctcaaatgct gtcacatgag tcgcggcacc tcgccgaagg tgccaaccac gcggtgctga
      121 ccactctgtt tccgagtgga cggccccaga ccaacgtcgt gtggatcgac cacgacggtg
      181 aacacttgtt ggtgaactcg gagaccgaga ggcagaaggt gcggaacgtg cggagggatc
      241 ccagggtcgc agtgttggta ttcgatgcct ccgacatgga caactgggtc gaggtcaggg
      301 gtcaggtggt cgagatcgtc gagggcgaag aagcgcggcg gcacatcgac acgctggcgc
      361 agaagtacct cggcgtgccc gactatccca acccgatacg atcgccgcgg gtgattctca
      421 agattcgccc agaccgggag aacgtctact gactcttcac gagccgacgc gcagggtcgc
      481 cgttcgcttt cgaagcatgc tgatcacgga cggtgccgtg atacgcccgg tacgggggtt
      541 ctccgacgga atgttctcga tggtcaaggt gaggtcggcg acatctgagc ccatgacgat
      601 ggtgtgagtg ttcttcgtca cccccggatc agcccagact tcgacctgcg tctcatcggg
      661 accaggcccg gccagggcga gcgcggccgc cacattcaga ttggcgggga agcccttcgc
      721 ggcatcccgg gcagtgccgg agaacacacg gaccggttcg gtcagattgt ccagatcgat
      781 tccctcgacc cccggcgccc cggccagtcc acgtggcggt ttgcgcgtga ccagtcgcag
      841 cgagctgatc tcgccctcag ccgccgccgc gatcgcatcg agacccacca gagcgccggt
      901 cggcaccgag atcaccccgc cgtgcgccct ggccaactcg atgagttcgg gatgctcgag
      961 gagagcgccg gcgctcaaga cgatgatctc cttccctgcc cggagtaggg gttcggcgat
     1021 ccgagggaac aatttcgctg gggcgcactc gatgaccagg tcagcgaggg atatgagctg
     1081 ttccacatcg accaccggca cgtccacccc gagcccttcg agcagttgct cagcgctctc
     1141 gcgatctcgc gccgaaaccg cggtgagctc gaagccaggc agtccgcgat ccagggccac
     1201 gaccagagca cgaccgaccg cgcccagacc gacaaccgcg actcgtgtca tccaagcgcc
     1261 gtctcagggg cgaacgtacc ggcataagcg gaggcaatct cgcccggggt ggtcaaccag
     1321 atatcgggga gacccaccag atactccaac gcggcgcgaa acaaccgcaa ccggtagggc
     1381 tggcccatga tgaaggtatg ggcgacgatc ccaaagacaa gcgattgacg ccgcgactgc
     1441 tcgaccatct cgtcgaggtt gtcagtcatc atgtcggcaa aagccggggc ggtcccgtgg
     1501 tggaacacga ccatcgggac atcattcacc tcatgtgggt agggaacgct gagtagcggc
     1561 ccgcccttgg tccgcatcca agtcggctgg tcgtccatgg gccagtcgag ggtatagaca
     1621 aacccgttct cggcgagaag gtcctcggtc gccgcgctgg gattcgcccc cgggctcatc
     1681 caacccgccg gaggtttgcc ttcgtgtgcg gacatcgtct tgacgattcc ccggatcatc
     1741 tccccttcct cgtgctcgtc caggtcgttc ggatggatgg aattggtgtg gccgtgggcg
     1801 acgaactcgg tctcggcgga tcgataggca tcgatcagct cggggcagtg gtcgtagcac
     1861 gatgtgttga tcagcgccgt cggcgtcact ccgatctgct cgaagacctc gagcaagcgc
     1921 cagcccccaa cccggttgcc gtactcccgc cacgcccagt tgtaggtgtt ggggtgcggg
     1981 atgcccggcg aataggacaa gccgaggccc ccctgcccat aggagaagtg ctcgatgcag
     2041 accgcgacgt agacggcgag ccgccgaccg ccgggccacg tgaagtcggg tcgcgacgtg
     2101 atcggggagt actcgaatcg tccgtgcgtg ggcagcatct cgggctctcc ttgcctatcg
     2161 gatcagggag tgtatgaacc cggcccgggt cggcggatcg ggcccaccgt ccgggatcta
     2221 cccttccgcc cggcatcgat cgaggaggac agccatggtg gagagaacgg cgatcacata
     2281 ccctggtcac gacgccggtt tgggcggtcg gatttggagt cctggcctga aggttggccc
     2341 ttggatcttc ctgtcaggga tcacgtcggt tgactacaac gagaagaaga cggtcggcgt
     2401 cgaagagcgg acgtcgatga ccccgcccaa gaaggatccg gaggctcagt ggcggcaggt
     2461 cctgagcaac atcaaggcaa tgatggagga ggccgggggg acgatggccg atgtggtcat
     2521 ggcaaacgtg ttcgtgaccg acatccacta ctactacgac tacgaatggg tccgaaagga
     2581 gttcttcgtg gagccctacc cggtgtgcac tgccgtcgag gtcggttcgc tcgtccatcc
     2641 cgactggatg ctcgagatcg aagtcatcgg atacatcgag ggctgacatg cgcggtggag
     2701 gatctgacag ctacaacctg gaggatttcg cctccgagct cgaggcggct ccggtgaatc
     2761 gggacgtcgg aaccaccctc tggttcgaga acgatcacgt ccgggtctgg gaggtgcgac
     2821 tcgaacccgg ccaaagggct ccgttccacg cccataccca aacctatttc tggacctgta
     2881 tcgatccggg caagggcttc cagcggttcc ccgacggcac tgctcaggcc ttcgacttcc
     2941 agcttggaga aaccgtcttc agtgagatca ctgcatcgga cccgatgatc cacgacctgg
     3001 agaacaccga cgatcaactc gtccgattcg tggcgatcga gctcaaggat ctctgaccgg
     3061 ccgacagtcg atgcagcaat acgacgtcgt catagttggc gggcgggttg ccggttcgac
     3121 cctggccagc ctcttgggtg ctggagggct cgaggtcctg ctgttggatc gagccgagtt
     3181 tcccgcagac accctctcga cacatgtcat ctacggggac tccttcggga tctgggagcg
     3241 catcggtgct tggggcgcaa tcgagcgcct cgcgtcgcag aaactctggg gcatctcgtg
     3301 gatccgggac gggaaacccg atatccgggg ccggttctgg cccgtccgtg gccacgacta
     3361 ctcgctctgc ctgcgacgcc tgcttctcga cgacgtgctc ttcaagaacg ctgcgaacac
     3421 tccgggcgta accgcgatcg aggcagcgcg ggtcaccgac atcctcctcg acggggacac
     3481 tgtcaacggc gtcaaattcg agacctcggg gtcgacaacc gaatcgaccg tcgcggcccg
     3541 gttggtcgtc gggtgtgacg ggcgccgatc caccatcgcc agggctgtcg gcgccaacca
     3601 atatgtaaca gagccgccga tcaacttcgc gttctacacc tacatccagg atgccgacac
     3661 cggacccgag ccggaaccga tgttcgagat ctgggacagc gaatacaacg gcggcacgcc
     3721 gatgctggcc gaatgtgacg ccgctgtatg gatggccatc gtctacctcc cgcaggagca
     3781 tttcgactcg ttccgcgccg acaaggacgg aaacttctgg gaggcgatgg accgcgaccc
     3841 gcgagtcggg ccgcgcatcc gggccgggcg gcagctgacc ccgatcaaag gtgccggcga
     3901 cttcgtcaac ttcgtccgcg acccggtcgg cgatggatgg gccctcgtgg gcgatgccgg
     3961 tcagcacaag gatcccatat tcggccaggg catcggcgac gccgtcaggt cggcagaggc
     4021 ccttgccgac tgcctcctgg aggcggaagc ggcgtcctcc gactggggct ccgcgctgaa
     4081 gaggtaccgg acgaaacggg acctggacct gatcccgaac ttccagtgga tgatccaggg
     4141 gaagcccgcc gacctcaccg aaggggactt caacctgatc atggacagcc tggggtcgga
     4201 cccggaccaa gccgagaggt tcgtgaacgt gttcagtcat gccgtgtccg ggtcggagtt
     4261 cttcgggcgc gtcaatgcgt cgcgacttct ggggagagac gctgccacac tcgatagccg
     4321 cagaatgctc acatcgagtg ggaaggaagg tgactagatg ggagccgaag accgcatcac
     4381 cgacctgggt taccgcctgg gtgagtctcc cccacccctc ggccaatacg tgcgatgtgt
     4441 cgtggcgaat gggctggcct tcacagcggg acacggtcca ttcgacgaat cggggaaggt
     4501 gagcattcgg ggccaggtcg gaggcgattt gtcactcgag cagggccagg aggcggctcg
     4561 cctggcggtg gtcgcctgcc tcgccagtct gaaggcggag ctgggctcac tggacgaagt
     4621 ggaacggatc gtgaagatgc tcgccttcgt caactgcgct cccggattcg tcaacacctc
     4681 cggggtgatc gacggagcgt cggacctgct ggtggacgtc tttggccccg ctggagtcca
     4741 tgcccgagcc gcgatcggga cgacggtgct ccctgggggc atcccggtcg agatagagct
     4801 catcgtcgag gtcagagatt cgagctaggc aggaggagcg ctgccgagga ggcaaggatg
     4861 cgacggcggc ttcaggtcac gagcacgtcg accttttcag gtgggttgac gcgaaccaat
     4921 ccttcctccg acaactt
//
LOCUS       DASTXO010000440         8526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_615769, whole genome shotgun sequence.
ACCESSION   DASTXO010000440 DASTXO010000000
VERSION     DASTXO010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8526
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615769"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /gene="fusA"
                     /locus_tag="VFU96_09270"
     CDS             complement(<1..1284)
                     /gene="fusA"
                     /locus_tag="VFU96_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020491310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU5113497.1"
                     /translation="MSNGNAAISHLRQVPLSRTRNIGIMAHIDAGKTTLTERILYYTG
                     RNYKIGEVHEGAATMDWMEQEQERGITITSAATTCVWHEHLINIIDTPGHVDFTVEVE
                     RSLRVLDGAIAVFDGVAGVEPQSETVWRQADRYGVPRICFINKLDRTGADFYFDVQSI
                     IDRLGARPLVLQLPIGSEADFTGVVDLVEMKAHIWKGDDGKHWDTTDIPADLADRAAE
                     YREKLLEAVAETSEELLDAYLENGDLTSKEIQEGVRHGTIARDFTPVFCGSAFKNKGV
                     QLLLDAVVAYLPSPADLPAIKGFRPGDEEVEVERQPEDGEPFAALAFKIMSDPHVGKL
                     TYFRVYSGHAAKGDTVLNTTTGQKERLGRILRMHANSREDIDDVFAGDIVAGIGFKNT
                     TTGDTLSDQGKAIQLESMEFPAPVISVAIEPKTKSD"
     gene            complement(1390..1860)
                     /gene="rpsG"
                     /locus_tag="VFU96_09275"
     CDS             complement(1390..1860)
                     /gene="rpsG"
                     /locus_tag="VFU96_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEU5113498.1"
                     /translation="MMRRAPAQKRKIDPDPVFRSDLVSKVINKVLWKGKKNTAREIVY
                     GALEVVEQRTSQDPSVILKRAVDNVRPQVEVRSRRVGGSSYQVPVEVSARRQETLAIR
                     WLVNFARQRREPTMAMRLANEILDASNYTGAAIKRKEDVHKMAESNKAFAHYRW"
     gene            complement(1857..2228)
                     /gene="rpsL"
                     /locus_tag="VFU96_09280"
     CDS             complement(1857..2228)
                     /gene="rpsL"
                     /locus_tag="VFU96_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005170078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEU5113499.1"
                     /translation="MPTIEQLVRGGRKPKKKKAKTAALGGAPQRRGVCTRVYTVTPKK
                     PNSALRKVARVRLSSQVEVTAYIPGEGHNLQEHSIVLVRGGRVRDLPGVRYKVIRGAL
                     DASGVNDRKQGRSRYGSKKVS"
     gene            complement(2406..3338)
                     /locus_tag="VFU96_09285"
     CDS             complement(2406..3338)
                     /locus_tag="VFU96_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113500.1"
                     /translation="MNASPGPPPGKGAKRLSPDELAQEVASLLKKDGPRHAERSRRFD
                     VVSLSRVLMAVTVVLFGVSVAVAVPWIGGLAGHATPGIVRHGGPVFGCPGEIAIGEVF
                     KGETVHVVGVSDNGLFYVLRDERGPDDVVYADVRAVTNVENPERLSTRGCEPRDDAVV
                     LAAATTLGLSGSIPTTTTTTPTTLTGEVLTTTTIPEVSPGVTVGRPPRRGTSTPGAPT
                     TTTTPPSTAPAGPGPTTTARPVSPTTTRPPSPPSTSPTTTTTTRPPTTSSSTTSITTS
                     TTTTTQPTTTTTEPTTTTTEATTTTEAEPTTTEP"
     gene            complement(3471..7394)
                     /locus_tag="VFU96_09290"
     CDS             complement(3471..7394)
                     /locus_tag="VFU96_09290"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU5113501.1"
                     /translation="MAQQLFDEIKIGLASSDQIRAWSYGEVKKPETINYRTLRPEKEG
                     LFDERIFGPQRDWECYCGKYKRIRYKGIICERCGVEVTRSKVRRERMGHIELAAPVTH
                     IWFFKGVPSRLGYLLDMSPKELEKVIYFASYIITSVDDEKRQKDLPELEAAMRDEIEV
                     VRSEFSEWHQVRLEALEDELERMEEGGAKSQEINARKKEVEREIKDRQERSDREVELI
                     EDAYDTFRKFKPKDLVESEQLWREIYFRYADYFEGGMGAEAVKALLTHLDLDTEMNSL
                     EENLDAGSAQKRQRATQRMKVVKPFWEGENRPEDMILETVPVIPPDLRPMVQLDGGRF
                     ATSDLNDLYRRVINRNNRLKRLLDLGAPEIIVNNEKRMLQEAVDALFDNGRRGRAVTG
                     PGNRALKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKIMALEL
                     FKPFVMKRLVDLDLAQNIKAAKRMVERQRPQVWDVLSDVIQEHPVLLNRAPTLHRLGI
                     QAFEPVLVEGKAIQIHPLVCEAFNADFDGDQMAVHLPLSAEAQAEARILMLSSNNVLS
                     PASGRPIVTPSQDMVIGIYYLSELIEEAKGAGRSFADFDEAMMAHEIGELSVHAPIKI
                     RLGDLAGDPELHADLKPTLGSLLTDAPLSNQGVSETTLGRVIINQVLPEGFPFVDSVV
                     IKADIRKLIETVIERYPRSAVAETLDGIKELGFKFATKAGLTIALEDVRTPADKAQIL
                     EEYEERAEKVQSLFQKGVVTDEERRQELIEIWTEATEKVTDAMKGGLKSERFNALDMM
                     VGSGARGNMMQVRQIAGMRGLVANPKGDIIPRPIISNFREGLSVLEYFISTHGARKGL
                     ADTALRTADSGYLTRRLVDVSQEMIILEADCETDKGIVISVVDERGSKIRNLRSRIFG
                     RALADDLKEGRTLVATVDDEKLRAGTVLGTKELHAIEGADIESVRVRSPLTCDTRGGV
                     CQACYGLSLATGKMVETGEAVGIMAAQSIGEPGTQLTMRTFHTGGVAGEDITHGLPRV
                     VELFEARTPKGKAVISDASGVVNLVEDSDGRRIVVENGEDEAIYAISRYAKLRVANGA
                     EIEAGTQLTEGPLDPKEVLEIRGVRATQQYLVDQVQEVYRSQGVEIHDKHIEMIVRQM
                     LRRVRVVAPSDSDFLPAALIDDQHFRDANKELVTEGKRPAEGRPELMGITKASLATDS
                     WLSAASFQETTRVLTDAAIQGRSDFMRGLKENVIIGKLIPAGTGAQQYQVLQPAMPGA
                     TTVASLGYLEGGLIAPGEDEGLPEDPAEWLASLGGTLGGGEDEEE"
     gene            complement(7446..>8526)
                     /locus_tag="VFU96_09295"
     CDS             complement(7446..>8526)
                     /locus_tag="VFU96_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU5113502.1"
                     /translation="VTPKGETELTPEERLLRAIFGEKAREVRDTSLKVPHGETGKVIA
                     VKVFKRAEGYDLPPGVNELVRIYVAQQRKISEGDKLAGRHGNKGVISKVLPEEEMPFL
                     ADGTPVDIILSPLGVPSRMNLGQVLETHLGWAAAQGWEPFDGKSPAVGGVKPWTKVAT
                     PVFDGAREDEIMQVIQSARPNRDGDRLVGDDGKAYLIDGRTGEPFDSPVTVGYIYMLK
                     LVHLVDEKIHARSTGPYSMITQQPLGGKAQFGGQRFGEMEVWALEAYGAAYALQELLT
                     IKSDDVQGRVKVYEAIVKGDNIPEPGIPESFRVLVKEMQSLCLNVEMLSAGEEIELRE
                     LEEESYRSVETLGIDLSRPERPDSE"
BASE COUNT         1353 a         2714 c         2760 g         1699 t
ORIGIN      
        1 gtcggacttg gtcttgggct cgatggcgac cgagatcacg ggtgcgggga actccatcga
       61 ctcgagctgg atcgccttgc cctggtctga cagcgtgtca ccagtggtgg tgttcttgaa
      121 cccgatcccg gccacgatgt ccccggcgaa cacatcgtcg atgtcctcgc gggagttggc
      181 gtgcatccga aggattcgcc cgagacgctc tttttggccg gtcgtggtgt tgagcaccgt
      241 gtcgcccttg gcggcatggc ccgaatacac ccgaaaatag gtcaacttgc cgacgtgcgg
      301 gtcggacatg atcttgaagg caagggcggc aaacggctcg ccgtcctcgg gctgccgctc
      361 gacctcgacc tcttcgtcgc cgggccggaa gcccttgatc gccggcaggt cggcgggact
      421 gggcagatag gcgactaccg catcgagaag gagctggact cccttgttct tgaaggcgga
      481 cccgcagaag accggggtga agtcgcgggc gatggtgccg tggcgaactc cctcctggat
      541 ctctttggag gtcaagtcac cattctcgag atatgcgtcg aggagctcct cggaagtctc
      601 cgcgaccgcc tcgagcagct tctcccgata ctcggcggcc cggtcggcga ggtcggccgg
      661 gatgtcggtg gtgtcccagt gcttcccgtc gtcgcccttc cagatgtggg ccttcatctc
      721 gaccaggtcg acgacgccgg tgaagtctgc ctcggacccg atcgggagct ggagcacgag
      781 cggccgagcc ccgagccgat cgatgatcga ctggacgtcg aagtagaaat cggcgccggt
      841 gcggtcgagc ttgttgatga aacagatgcg gggcacgccg taacggtcgg cctggcgcca
      901 caccgtctcc gactgaggct cgactccggc gacgccgtcg aacacggcaa tggctccgtc
      961 gaggacacgg agggaccgct ccacctcgac cgtgaagtcg acgtgacccg gggtatcgat
     1021 gatgttgatc aggtgctcgt gccagacgca ggtggtggcg gcggaggtga tggtgatgcc
     1081 acgctcctgc tcctgctcca tccagtccat cgtggcggcg ccttcgtgga cctcaccgat
     1141 cttgtagttg cggccggtgt aatagaggat gcgctcggtc aaggtggtct tgcccgcgtc
     1201 gatgtgagcc atgatcccga tgtttcgggt tcggctcagc ggcacctggc gcagatgcga
     1261 tatggcagcg tttccgttgc tcattctctt cttctcttac tccgactcga tatgcggttg
     1321 tgaacgtccc acgtcctgcg gttcgggcct gtttcggtgt cgggcccgcc tgctggaacg
     1381 ttcctcgggt taccagcggt agtgggcgaa ggccttgttg gactccgcca tcttgtgaac
     1441 atcttccttg cgcttgatcg cggccccggt gtagttcgag gcgtccagga tctcgttggc
     1501 gagacgcatc gccatggtgg gctcgcggcg ctgtctggcg aagttgacca accagcggat
     1561 ggccagcgtc tcctgacgcc gggccgagac ctcgacaggc acctggtagg agcttccacc
     1621 gacccggcgg gaccgcacct cgacctgggg tcgcacattg tccaccgctc gcttcaggat
     1681 cacgctcggg tcctggctgg tgcgctgctc gacgacttcg agggctccgt acacgatctc
     1741 ccgagcggtg ttcttcttcc ccttccacaa gaccttgttg atgaccttgc tgacgaggtc
     1801 gctgcgaaat accggatccg gatcgatctt gcgcttctgt gccggtgctc gtcgcatcag
     1861 gagaccttct tcgatccgta acgactgcgg ccctgtttgc gatcgttgac acccgatgca
     1921 tcgagagcgc cccggatgac cttgtagcgc acacctggca agtcacgcac ccggccgccg
     1981 cggaccaaga cgatgctgtg ctcctggagg ttgtgaccct cgccggggat gtaggcggtg
     2041 acctcgacct gagaggacag gcgaacacgg gccaccttgc gcagtgccga gttcggcttc
     2101 ttgggcgtca ccgtatagac ccgcgtgcac acgccgcgcc gctgcggggc gccgccgagt
     2161 gcagccgtct tcgctttctt cttctttggc ttcctgcccc cgcggacaag ttgctcgatc
     2221 gtcggcatgt ttctcctata cgccaggaaa cgacacgggc acaccccaag cgtcgtcctg
     2281 atgtgaaacc ttgcgtaccg ggcactggat gtgcccggcc catccggtcg cgctcaataa
     2341 acgagcggcc ccggacgaag cacggattct acgtgccttc tccgattagg gccaaatcga
     2401 tttgatcacg gctcagtggt tgtgggctcc gcctcggtgg tggtggttgc ctcggtcgtg
     2461 gtggtggttg gctccgtcgt ggtagtcgtg ggttgcgtag ttgtcgtggt cgatgtcgtg
     2521 atgctcgtgg tcgacgacga cgtggtggga ggccgggtcg tggtggtcgt ggtcgggctg
     2581 gtcgatggag ggctcggcgg ccgggtggtg gtcggagaca ccgggcgagc ggtggtcgtc
     2641 ggtccgggac cggctggggc ggtggacggc ggtgtggtcg tcgtcgttgg tgcgccaggt
     2701 gtcgacgtgc cacgtcgtgg tggtcgcccg acggtcacac cagggctcac ctccgggatg
     2761 gtggtcgtgg tgaggacttc gcccgtcaga gtcgttggcg ttgtggtggt cgtggttggt
     2821 atggaaccgc tcaggcccag cgtggtggct gcagccagaa cgacggcatc atcccgaggc
     2881 tcacacccac gtgtcgacag tcgctcggga ttctcgacat tcgtgacggc ccgaacgtcg
     2941 gcgtagacga cgtcatcagg cccgcgttcg tcgcgcaaga cgtagaagag cccgttatca
     3001 ctgacgccga ccacgtgcac ggtctcgcct ttgaacacct caccgattgc gatctcccca
     3061 gggcatccga agaccggtcc gccgtgtctc acgatgcccg gggtcgcatg gccggcaaga
     3121 ccaccgatcc agggcacggc aacggccaca cttaccccga aaagtacgac ggtgaccgcc
     3181 atgaggaccc gactgagcga tacgacgtcg aatcgccggg accgctcggc atgccgcggc
     3241 ccgtccttct tcaagaggga cgcgacctcc tgggcgagct catccgggga caggcgcttg
     3301 gcacctttcc cgggcggcgg acctgggctg gcattcatcg acgggacctc gtttgaccgc
     3361 gcagccaccc gtcccgccgc aggaaaagct attgcctgca gattccgtgt caacaaagaa
     3421 agaaccgccc attcgggcgg ttctttcgaa ggtcattcag gcccggggcc ctactcctct
     3481 tcgtcctcgc cgccgcccag ggtgccaccc aaggaggcaa gccattcggc ggggtcctcg
     3541 gggaggcctt cgtcttcgcc aggggcgatg agcccaccct cgaggtaccc gagcgaggca
     3601 acggttgtgg cgccgggcat cgcaggctgg agcacctgat actgctgggc gccggtgccg
     3661 gccgggatca gcttgccgat gatcacgttc tccttgaggc ccctcatgaa gtcggagcgg
     3721 ccctggatgg cggcatcggt cagcactcga gtcgtctcct ggaacgaggc cgccgacaac
     3781 cacgagtccg tcgccagaga ggccttggtg attcccatga gctcgggccg gccctcggcc
     3841 gggcgcttgc cctcggtcac cagttccttg ttggcatcac ggaaatgctg gtcatcgatc
     3901 agagcggccg gcaggaagtc ggagtcgctc ggggcgacca ccctgacccg gcgcaacatc
     3961 tgtctcacga tcatctcgat gtgcttgtcg tggatctcca caccctgcga ccggtatacc
     4021 tcttggactt ggtcgacgag gtattgctgg gtggcgcgga cgccccggat ctcgaggact
     4081 tccttggggt cgagcggacc ttcggtcagc tgagtccccg cctcgatctc ggcgccattg
     4141 gcgacgcgaa gcttggcata acgggagatg gcatagatcg cctcgtcctc gccgttctcc
     4201 acgacgatgc gccggccatc cgagtcctcg acgaggttga ccacgccgct ggcatcggag
     4261 atgactgcct tccccttcgg agtacgagcc tcgaacagct cgaccacccg gggcaggcca
     4321 tgggtgatgt cctccccggc aacgcctccg gtgtggaagg tccgcatggt gagctgggtt
     4381 ccgggctctc cgatcgactg tgcagccatg atgccgaccg cctcgccggt ctccaccatc
     4441 ttccccgtgg cgagggacag gccgtagcag gcctggcaca cgccacctcg ggtgtcacag
     4501 gtgagcggtg agcggacccg aaccgactcg atgtcggccc cctcgattgc gtgaagctcc
     4561 ttggtgccca gcacggtgcc ggcccgaagc ttctcgtcgt cgacggttgc aaccaaggtg
     4621 cggccctcct tgagatcgtc ggccagagcg cgaccgaaga tccgggaccg gaggttgcgg
     4681 atcttgctcc ctcgctcgtc gaccaccgag atgacgatcc ccttgtctgt ttcgcagtcg
     4741 gcctcgagga tgatcatctc ctgggagacg tcgacgaggc gacgggtcag ataaccggag
     4801 tcggctgtgc ggagcgcggt gtcggcgaga ccctttcgag ccccgtgagt cgagatgaag
     4861 tactcgagga ccgacagacc ctcgcggaag ttggagatga tggggcgggg aatgatgtca
     4921 cccttcgggt tggccaccag accgcgcatg cccgcgatct gacgcacctg catcatgttc
     4981 ccgcgggccc cggacccgac catcatgtcg agagcgttga agcgctcgga cttgaggccg
     5041 cctttcatgg cgtcggtcac cttctcggtg gcctcggtcc agatctcgat cagctcctgg
     5101 cgacgctcct catccgtgac gacccccttc tgaaagagac tctgcacctt ctcggcccgc
     5161 tcctcgtact cctcgaggat ctgggccttg tcggccggag tccgaacgtc ctcgagggcg
     5221 atggtgagac cggccttcgt ggcgaacttg aagccgagct ccttgatgcc gtcgagggtc
     5281 tcggccaccg cggaccgcgg gtagcgctcg atcacggttt cgatcagctt gcggatgtcc
     5341 gccttgatca ccaccgagtc gacgaacggg aacccttcag gcaggacctg attgatgatg
     5401 accctgccga gtgtggtctc cgagactccc tggttgctca ggggcgcgtc ggtgagcagg
     5461 ctccccaacg tcggcttgag gtcggcgtgc aactccggat cgccggcgag gtcgccgaga
     5521 cggatcttga tcggagcgtg taccgacagc tcgccgatct cgtgggccat catcgcctcg
     5581 tcgaagtcgg cgaacgaccg accggcgcct tttgcctcct cgatcagctc tgacaggtag
     5641 tagatgccga tgaccatgtc ctgcgaagga gtcacgatcg ggcgcccgga ggcgggcgac
     5701 agcacattat tcgagctgag catgaggatt ctggcctcgg cctgggcctc ggcggagagc
     5761 ggcaggtgga cggccatctg gtcgccgtcg aagtcggcgt tgaaggcctc acacaccagc
     5821 gggtggatct ggatcgcctt gccctcgacc agcacaggct cgaaggcctg gatcccgaga
     5881 cggtgcaggg tgggcgctcg gttgagcaac accggatgct cctggatcac gtccgacaag
     5941 acgtcccaga cctgggggcg ctgccgttcc accatccgct tggcagcctt gatgttctgc
     6001 gccaggtcga ggtcgaccag tcgcttcatc acgaacggct tgaacagctc gagggccatg
     6061 atcttgggca gaccgcactg gtgcagcttg agctgaggcc cgaccacgat gaccgagcgg
     6121 cccgagtagt cgacccgctt gcccagcagg ttctgacgga accggccctg cttgcccttg
     6181 agcatgtcgg acagcgactt gagggcccgg ttgcccggac cggtcaccgc cctgccccga
     6241 cggccgttgt cgaacagggc gtcgacggcc tcctggagca ttcgcttctc gttgttgacg
     6301 atgatctcgg gggcgccgag atcgaggagg cgcttgagac ggttgttccg gttgatgacc
     6361 ctgcggtaga gatcgttgag gtcggaagtg gcgaaacgac cgccatcgag ctgaaccatg
     6421 ggcctgaggt cgggtgggat gaccgggacc gtctcgagga tcatgtcctc gggccggttc
     6481 tcgccttccc agaacggctt gaccaccttc atcctctgag tggcccgctg acgcttctgg
     6541 gccgagccgg cgtcgaggtt ctcctcgagg ctgttcatct cggtgtcgag gtcgagatgt
     6601 gtgagcaagg ccttgaccgc ctctgccccc atgccgccct cgaagtagtc ggcgtaacgg
     6661 aagtagatct cacgccacag ctgctcggac tcgaccaggt ccttcggctt gaacttgcgg
     6721 aaggtgtcgt aggcgtcctc gatcagctcg acctcgcggt cggagcgctc ctgacggtcc
     6781 ttgatctccc gctcgacttc cttcttgcgg gcgttgatct cctggctctt cgccccgcct
     6841 tcctccatgc gctcgagctc gtcctcgagg gcctcgagcc ggacctgatg ccattcgctg
     6901 aactcggagc ggaccacctc gatctcgtcg cgcatcgcgg cctcgagctc gggcaaatcc
     6961 ttctgccgct tctcgtcatc gaccgaggtg atgatgtagc tggcgaagta gatcaccttc
     7021 tccagctcct tgggcgacat gtcgaggaga tagcccagac ggctgggcac acccttgaag
     7081 aaccagatgt gggttaccgg ggcagccagc tcgatgtggc ccatgcgctc gcgcctcacc
     7141 ttggaacgag tcacctcgac accgcagcgc tcacagatga tccccttgta gcggatcctc
     7201 ttgtacttgc cgcaatagca ctcccagtcc cgctggggac cgaagatgcg ctcgtcgaag
     7261 agaccctctt tctcgggacg gagggtgcgg tagttgatcg tctccggctt cttgacctct
     7321 ccgtaggacc aggcccggat ctggtcgctg gaggccaggc cgatctttat ctcatcgaat
     7381 aattgctggg ccattaacgc tcgctttgct cgctgttcta cgtttttacg ttctacgtct
     7441 tgcgactatt ccgagtcggg gcgctcgggt cggctcaggt cgatgccgag ggtctcgacg
     7501 gagcgatacg actcctcctc gagctccctg agctcgatct cctcaccggc ggagagcatc
     7561 tccacgttga ggcagaggct ctgcatctcc ttcaccaaca cccgaaacga ctcgggaata
     7621 ccgggctcgg ggatgttgtc gcccttgacg atcgcctcgt agaccttgac ccggccctga
     7681 acgtcgtcgg acttgatggt gagcagttcc tggagggcat aggcggcgcc ataggcctcg
     7741 agggcccaca cctccatctc accgaacctc tgaccgccga actgggcctt accacctagc
     7801 ggctgctggg tgatcatgct gtacgggccg gtcgaccgag cgtggatctt ctcgtccacg
     7861 aggtggacca gcttcaacat gtagatgtag ccaaccgtga ccgggctgtc gaagggctcg
     7921 ccggtgcgac catcgatgag gtaggccttc ccgtcgtcgc ctaccagccg gtcgccgtcc
     7981 cggttggggc gtgcgctctg gatgacctgc atgatctcgt cctcgcgggc accgtcgaaa
     8041 acgggcgtgg ccaccttggt ccagggcttg acgcccccga ccgccgggct cttcccgtcg
     8101 aagggctccc aaccctgggc agcagcccat ccaaggtgag tctcgaggac ttgccccaag
     8161 ttcatccggg aggggacgcc gagcggggag aggatgatgt caaccggggt gccgtcggcc
     8221 aggaatggca tctcctcctc ggggaggacc ttggagatga cgcccttgtt cccgtgtcgg
     8281 ccggccagct tgtctccttc ggagatcttc ctctgctggg cgacgtagat cctgaccaac
     8341 tcgttgaccc ctggtgggag gtcgtatccc tcggcgcgct tgaagacctt gaccgcgatc
     8401 accttgccgg tctcgccgtg gggaaccttg agtgatgtgt cgcggacctc tcgagccttc
     8461 tcaccgaaga tggcgcgcag aaggcgctct tcgggcgtga gctcggtctc acccttgggg
     8521 gtgacc
//
LOCUS       DASTXO010000441         2167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_394410, whole genome shotgun sequence.
ACCESSION   DASTXO010000441 DASTXO010000000
VERSION     DASTXO010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394410"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..1259)
                     /locus_tag="VFU96_09300"
     CDS             complement(36..1259)
                     /locus_tag="VFU96_09300"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5113503.1"
                     /translation="MPEADSARNRLTAVLFGGVALGSTAYIAAVTIATLAVDEITGSA
                     ALAGVPGATATLGTAVGTSLLTRNVARRGRRPGLAAGYAAAVVGSALSIFGLTSDSLP
                     LLLMGMALLGLGNASGHLARYAAAEMFPEDRRGRALGTVVWAATIGSVLGPALLQPSG
                     RLAIDLGRSELAGGFLVSLVFMGIALVLYLVALRPDPVTLAIDRPQPTDEPPVGLGPA
                     FRLPLVRVALTAMIAGQVVMVMIMTSTPLHIHHHGSDLGIVGLVMSAHTLGMFALSPL
                     TGRLTDRWGGYRVIMGGMVLLGLSALGAAYGPNSSTALLVVVLFALGYGWNLAFVAGS
                     SMLTIGVGSDIRTRLQGRVDSLTWTSGALASVSSGVIYQASDYRAISLIGLFLLVIPV
                     VVVLRHRGSPLAVGA"
     gene            complement(1314..>2167)
                     /locus_tag="VFU96_09305"
     CDS             complement(1314..>2167)
                     /locus_tag="VFU96_09305"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEU5113504.1"
                     /translation="PSTSDTTVPPPGEDPLYAPAAPGPSGDPFAAMPSGAEIVTVISV
                     TDGDTLDVRFPGGSVFEVRIIGTNSPESGECFSDEATQVLAALAPVGSQIGMTSDVSD
                     FDQFDRLLRYLWVGGMSVNEETVRRGAAISRSYPPDTAMTDRFDEAQAAATGAGRGLW
                     SPTACGPRAEANLSVADLNYDAPGDDNQNLNEEWIAIRNDGAAPVDLTGWGIKDESAS
                     HRYSFPPSFTLFPGETVVVRTGCGTDFDTDLYWCNQGSAVWNNGGDTAFLLDPNGNTH
                     VAYAYSG"
BASE COUNT          406 a          718 c          730 g          313 t
ORIGIN      
        1 cgaccactcc ggatcgaggg ctgagctccc tgcactcagg cgccgaccgc cagcggggag
       61 ccccgatggc ggagcacaac cacgacgggt atgaccagta ggaacagccc gatcagagag
      121 atggcccggt aatcgctggc ctggtagatc acgcccgagg agacgctggc cagggcaccg
      181 gaggtccagg tgagcgagtc caccctcccc tggagacgag tcctgatgtc actgcccacg
      241 ccgatggtga gcatcgagct accagcgacg aaggccagat tccagccgta accgagggcg
      301 aacaggacca ccaccagcag agccgtggag gagttggggc catatgccgc ccccaacgcc
      361 gagagcccga gcagaaccat gcctcccatg atcacccgat acccgcccca tcggtcggtg
      421 agcctgccgg tgagagggga caaggcgaac atgcccaggg tgtgggcgct catcacgagg
      481 ccgacgatcc ccagatcgga gccgtgatgg tggatgtgga ggggggtcga ggtcatgatc
      541 atcaccatca ccacctggcc ggcgatcatc gcggtcaggg cgacacgcac caacgggagg
      601 cggaacgccg gccccaaacc aacgggcggc tcgtcggtcg gctgtggtcg atcgatggcg
      661 agggtgaccg ggtcgggccg cagggccacc aggtagagaa ccagggcgat tcccatgaac
      721 accaggctca ccaggaagcc accggccagc tccgaccggc ccagatcgat cgcaaggcgg
      781 cccgatggct gaagcagggc cgggcccaac accgatccga tcgtcgccgc ccagaccacc
      841 gtgcccaagg cccggcccct gcggtcctca gggaacatct ctgccgcggc gtatcgggcc
      901 aggtggcccg atgcgttgcc tagaccgagg agcgccatcc ccatcagtag gaggggaagg
      961 gagtctgagg tcagcccgaa gatgctcaac gcgctgccca cgactgccgc ggcatagccc
     1021 gccgccaatc ctgggcgacg gccacgacgc gccacgtttc gagtgaggag actggtccct
     1081 accgcggttc cgagggtggc cgttgcccca ggcacacctg ccaaggcggc agagcccgtg
     1141 atctcgtcca cggcgagggt ggcgatcgtc accgccgcga tgtaggccgt cgagccgaga
     1201 gcgacgccac cgaacaggac agcggtgaga cgattgcgtg cggagtcggc ttccggcatc
     1261 tgggtcagtt tggtcgcccc agtcttgccc ggagccggat atggaatgcc tcctcacccc
     1321 gaataggcgt aggcgacgtg ggtgttgccg ttgggatcga ggaggaaggc ggtgtcgccc
     1381 ccgttgttcc acaccgccga gccttgattg caccagtaga gatcggtgtc gaagtcggtc
     1441 ccacaaccgg tcctgacgac gaccgtctca cccgggaaca gggtgaacga aggcgggaag
     1501 gagtaccgat ggctggccga ctcgtccttg atgccccatc ccgtgaggtc gaccggggca
     1561 gctccgtcgt ttcggatcgc gatccattcc tcgttgaggt tctggttgtc gtctcccgga
     1621 gcgtcgtagt tcaagtcggc gacactcaga ttggcctcag ctcgcgggcc gcaggctgtc
     1681 ggtgaccaca agccccgccc cgcccccgtg gccgcggcct gggcttcgtc gaaccggtca
     1741 gtcattgccg tgtccggggg atagctcctc gagatggctg ccccacgacg caccgtctcc
     1801 tcgttgaccg acattccccc tacccacaga taacgaagca gacggtcgaa ctggtcgaag
     1861 tcggacacgt cgctggtcat cccgatctgc gacccgacgg gcgccagggc ggcgagaacc
     1921 tgcgtcgcct cgtccgagaa gcactcaccg gactcgggtg aattggtccc gatgatcctg
     1981 acctcgaaca ccgacccgcc ggggaaccga acgtcgagag tgtctccgtc tgtcaccgag
     2041 atcacggtca cgatctcggc accggatggc atcgccgcga aggggtcacc ggagggcccg
     2101 ggggcagccg gcgcgtagag agggtcctcg cccggcgggg gcacagtcgt gtcggatgtc
     2161 gaagggg
//
LOCUS       DASTXO010000442         3603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_578883, whole genome shotgun sequence.
ACCESSION   DASTXO010000442 DASTXO010000000
VERSION     DASTXO010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578883"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /gene="aroA"
                     /locus_tag="VFU96_09310"
     CDS             <1..1019
                     /gene="aroA"
                     /locus_tag="VFU96_09310"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU5113505.1"
                     /translation="VDVGASGLTARSVIAVASLVHGTTTVVGRDRLPERPMGGLVDAL
                     DALGVDVTSVDGPLPVIIEGVGALSGGSVSVDSRHTSQFASALLLTAPLARGELIVTP
                     EGIEGSQRYLDMTVGMMKEFGATVDRVGSGYRVEPHGYTGRDIVIEPDASAAVYPMVA
                     AAITGGRVTIEGLGSESMQPDLQIAVVLERMGCDVRQSGHDTTIVGPLDGQLRPVDVD
                     LSGSPDGSLAVAVAALFAESPSKLRGLGSLRHKESDRLAALAIEIARLGGDAKVVRDE
                     LHITPGKLRPARVQTYGDHRVAMSFALVGLRIPAIEIADPEVVAKTWPAYWGMLSGLA
                     QSIA"
     gene            1168..1818
                     /gene="cmk"
                     /locus_tag="VFU96_09315"
     CDS             1168..1818
                     /gene="cmk"
                     /locus_tag="VFU96_09315"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEU5113506.1"
                     /translation="MSDLIVTLDGPAGTGKSTVSREVASRLGLPRLDTGAFYRAAALA
                     AIRAGVDLADHEAVANVVAGISLDQQDGRMLLDGLDVSVEIRSQAATEASSVVSTHPE
                     VRGLLVVYQREWVARHDNRAVVEGRDIGSVVFPDATVKVYLDARPEVRAQRRADQTGK
                     EHSGVLAELSARDARDSTRQASPLIIPEGAAVIDTSDMTVAEVVDTIVAMAREQAG"
     gene            1869..2918
                     /gene="ispH"
                     /locus_tag="VFU96_09320"
     CDS             1869..2918
                     /gene="ispH"
                     /locus_tag="VFU96_09320"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEU5113507.1"
                     /translation="MTIERVLVAEPRGFCAGVEMAIKALTWMTRIFDGPVYCYHEIVH
                     NEWVVKAFEGVGVVFVSDIDDVPEGAPVMLSAHGSAPEVVAGAAARAAVVIDAVCPLV
                     TKVHHEVKRMARDGFDIIYVGHEGHDEAVGTVAEAPSAIRLVEPETGLGEYAPSDPSK
                     VALLAQTTLGVHEWMAVMDDARVRFPDLQMSRKSDLCYATTNRQDAVKQLAGECDLVL
                     VVGSHNSSNTQALVRVARDHGVRAHRIDSADDIDAVWLDGVSVVGLTAGASAPDHLVQ
                     EVIDRLAPTAGFELWSAIEEQEYFPLPPQLRSFVTTLQNLVEAGVTARHPGRDGWLQA
                     DREWTATEALDVVSA"
     gene            2956..>3603
                     /locus_tag="VFU96_09325"
     CDS             2956..>3603
                     /locus_tag="VFU96_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEU5113508.1"
                     /translation="MSRLPVVVVVGRPNVGKSTLVNRIIGSRSAVVEELSGVTRDRRE
                     FDGDWSGHEFVLVDTGGWEMKPSDHMGASIKEQAEAAVSGADVLIFVVDGTSELTDDD
                     TGVISVLRSAEIPVVLAANKIDNETMEGNIDRLWGLGLGEPYPISAYHGRGMGELLDQ
                     VVAALPQPTDVEKGDEVPRVAIVGRPNVGKSTLLNKLVGERRVIVSETPGTTRDPI"
BASE COUNT          613 a         1118 c         1252 g          620 t
ORIGIN      
        1 cggtggacgt cggggcctcc ggtctgactg ccaggtcggt catcgccgtg gcgtccctcg
       61 tgcatggcac caccaccgtc gtcggtagag atcggctgcc cgagcggccc atgggcggcc
      121 tggtggatgc gctcgacgcc cttggcgtcg acgtgacgtc cgtcgacggc cctcttccgg
      181 tgatcatcga gggagtgggg gccctgtccg ggggttcggt ctcggtcgat tcccgtcata
      241 ccagtcagtt cgccagtgca ttgctcctca ctgctccctt ggcgcggggc gaactgatcg
      301 tgactcccga ggggatcgaa ggatcgcagc gctatctcga catgacggtc gggatgatga
      361 aggagtttgg ggcaacggtg gatcgggtcg gaagtggcta tcgcgtcgag cctcacgggt
      421 acaccggcag agacatcgtt atcgagcccg acgcgtcggc ggctgtctac ccgatggtgg
      481 ccgccgcaat caccgggggc cgcgtcacca tcgaggggct cggatcggaa tccatgcaac
      541 cggatctcca gatcgctgtc gtcctagagc gaatgggctg cgacgtgagg caatcgggcc
      601 atgacaccac gattgttggg cccctcgacg ggcagctgcg acccgtcgac gtcgatcttt
      661 ccgggtcgcc tgatggctcg cttgcagtgg cggtggccgc cctgttcgcc gaaagcccga
      721 gcaagctccg cgggctcgga tcgctccgcc acaaggagtc ggaccggctg gcggcattgg
      781 cgatcgagat cgcccggctc gggggggatg ccaaggtcgt tcgagatgag ctccacatca
      841 cgcccgggaa attgcgccct gcccgggtgc agacgtatgg ggatcaccgg gtcgcgatgt
      901 cctttgccct ggtggggctg cggatccccg caatcgagat tgccgacccc gaagtcgtgg
      961 ccaagacgtg gccggcctac tgggggatgc tgagtggcct cgcccaatcg attgcgtgaa
     1021 gagacggcgc gtcgggcccg cgccgcgcta ggttgcgatc cacggagagg accgacccgg
     1081 tcccacaagc ccccattgtc cgaatacccc cataggacac cctcagggat cgggtccctc
     1141 tccgcctgac cgatatgctc cccgcccgtg agcgacctca tcgtgaccct cgacggcccg
     1201 gcggggaccg ggaagagcac ggtgagcagg gaggtggcgt cacgtctcgg gttgccccgt
     1261 ctagacaccg gggcgttcta tcgggcggct gccctggccg caattcgagc cggtgtcgat
     1321 ctcgccgacc acgaggcagt ggcgaatgtc gttgccggga tcagcctcga tcagcaggac
     1381 ggccggatgc tcctcgatgg cttggatgtc tcggtggaga tccgcagtca ggccgcgaca
     1441 gaggcatcgt cggtggtatc cacgcacccc gaggtacgag gtctcttggt cgtctaccag
     1501 agggagtggg tggcccggca tgacaaccga gctgttgtcg agggccgcga catcgggtcg
     1561 gtcgtcttcc ccgacgcaac cgtgaaggtc tatctcgatg cccgcccgga ggtcagggcg
     1621 cagcgacgag ccgatcaaac gggcaaagag cattccggcg tgctcgcaga gttgagtgca
     1681 agggacgcca gggattccac acgccaggct tcgcctttga tcatccccga aggggcagcg
     1741 gtgatcgaca cttcggatat gacggtcgcc gaggtcgtcg acacgatcgt cgcaatggcc
     1801 cgcgagcaag cgggctgagg gggcgtggtc gaaggaggtt ggagccaggc cccacctacc
     1861 attgacctat gaccattgag cgggttctcg ttgccgagcc ccggggcttt tgcgccgggg
     1921 tggagatggc gatcaaggct ctgacctgga tgacccgaat cttcgatggc cccgtgtact
     1981 gctatcacga gatcgtccac aacgagtggg tggtgaaggc attcgagggc gtaggagtcg
     2041 tgttcgtcag cgacatcgac gacgtgccgg agggcgcgcc ggtgatgctt tcggctcacg
     2101 gttcagcccc cgaagtggta gccggggcag ccgcccgagc ggcagtggtc atcgacgccg
     2161 tttgccccct cgtcaccaag gtgcatcacg aggtgaagcg aatggcccgt gatggcttcg
     2221 acatcatcta tgtcggtcat gagggccatg acgaggcggt tgggacggtg gcggaggcgc
     2281 cttcggccat ccggctcgtc gagccggaga ccggccttgg cgagtacgcg ccctccgacc
     2341 cgagcaaggt cgccctcctg gcccagacga cgttgggtgt ccacgagtgg atggcggtga
     2401 tggacgacgc tcgcgttcgg ttccccgacc tacagatgtc ccggaagtcc gacctctgct
     2461 acgccacgac caaccggcag gacgcggtca agcaactggc cggcgaatgc gacctggtgc
     2521 tggtggtcgg gtcacacaac tcgtccaaca cccaggccct ggtgcgggtg gcgcgtgatc
     2581 atggggtgag agcccatcgc atcgactcgg cagacgacat cgatgccgtc tggctggacg
     2641 gcgtgtccgt ggtcggcctc accgcaggcg catcggcgcc cgatcacttg gtccaggagg
     2701 tcatcgatcg gctcgccccg acggcgggat tcgagttgtg gtcggctatc gaggaacagg
     2761 aatacttccc gcttccacca cagctcaggt cattcgtgac gaccctccag aacctggtcg
     2821 aggccggagt gacggctcgt caccctggac gggacggctg gctgcaggcg gaccgggagt
     2881 ggaccgccac cgaggccctc gacgtcgtca gcgcctgatc ccgcagcacg cgggcagaat
     2941 gcctcaggag ccttgatgag cagacttccc gtcgtcgtag tcgtgggtcg ccccaacgtg
     3001 ggtaagtcga ccctggtcaa tcgaatcatc ggttcgaggt ctgcggtggt cgaggaacta
     3061 tcgggtgtca cccgcgaccg ccgcgagttc gacggcgatt ggtccggcca tgagttcgtc
     3121 ctggtcgaca ccggcggatg ggagatgaag ccatccgatc acatgggcgc ctccatcaag
     3181 gagcaggcgg aggccgcggt atcgggcgcg gatgtgctga tcttcgtggt cgatgggacc
     3241 agcgagctca ccgatgacga caccggcgtg atctccgtgc tcaggtcggc agagatcccg
     3301 gtggtgctgg ccgccaacaa gatcgacaac gagacgatgg agggcaacat cgaccgactc
     3361 tggggcctgg ggctcgggga gccctatccg atcagcgcct accacggcag agggatggga
     3421 gagcttctgg accaggtagt cgccgcgctg ccgcagccga ccgacgtgga aaagggagac
     3481 gaggtgcctc gcgtggccat tgtcggccgg cccaacgtcg gaaagtcgac gctgctgaac
     3541 aagctggtcg gcgagcgccg ggtcatcgtc tccgagacac cggggacaac tcgtgaccca
     3601 atc
//
LOCUS       DASTXO010000443         3203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1131701, whole genome shotgun sequence.
ACCESSION   DASTXO010000443 DASTXO010000000
VERSION     DASTXO010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131701"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VFU96_09330"
     CDS             complement(<1..578)
                     /locus_tag="VFU96_09330"
                     /inference="COORDINATES: protein motif:HMM:NF017046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HEU5113509.1"
                     /translation="MSDPYAERRARLAEQIGDDAIAVIPAAVEVIRSNDTEFEFHQDS
                     DFLYLTGFHEPEAVAVITPGHADGDYTLFVRPRDPELEAWNGYRAGTEGAKERFGADH
                     AYEVGQLDEILTRRMIGREVIWYRTGNPHHDDRIAGIVAKARSHRERYGGSVPSAVRD
                     ITVLLAEMRLFKSREEIEILRRAGELSARGHG"
     gene            complement(629..1573)
                     /locus_tag="VFU96_09335"
     CDS             complement(629..1573)
                     /locus_tag="VFU96_09335"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU5113510.1"
                     /translation="MLPVILVVIPAAVIALISLASFAGAWVWWLDVMANFRAQYVIIL
                     AVLGLIVVASRWRRTGLAILGVALVNLFFVAPLFVGSPGDPVAGAPGMRVMSFNLLST
                     NESYGEVIEYIEATDPDLVLLHESSRPWEVAMESSGLDYEIVRGRSDNLIFGTLVLAR
                     DEVEAVSYGFAEGDGRAISLQYQPEGWPVPVTILSSHPLAPTSGERAALRDAQLAFAA
                     GWAAEQDGAYAVVGDLNASPWSSPFRGLVSEGNLRNSQIGFGLQPSFSAMTIFPLRVP
                     IDHLLHSEDLRVRDRRLGPALGSDHFPLVVDLEHAPGT"
     gene            complement(1575..2933)
                     /gene="argH"
                     /locus_tag="VFU96_09340"
     CDS             complement(1575..2933)
                     /gene="argH"
                     /locus_tag="VFU96_09340"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU5113511.1"
                     /translation="MSLWGGRFESGPGQVLWTFTTDDSDRRLLDDDITGSLAHVRMLG
                     KSGILTELEVNILVGGLETIRSETAGGVFEYSESDEDVHSAVERRLFELIGDTAGKLH
                     TARSRNDQVALDMRLYTRRVCGERIGQLHAYALGLAEIAERHAETVVPSYTHLQQAQP
                     TTLGHHLLAYAWMAVRDADRFAGVSTRIDVSPLGAGASAGSSLPIDIAATAGELGMSG
                     VFENSLDAVGSRDHLAEYVFCCAQAMVNLSRLAEEVIIWATSEFNRAKLGDRVSTGSS
                     ALPHKRNPDIAELVRGRAARVSGLLTSIMTLQKGLPLAYNRDLQEDKRLVFEADDTLA
                     QSLAAMVEMLRQTEFLPVDPNVETASLDLSEALVARGIPFREAHEAVGKLVLVLEGQG
                     RTLQEATADDLISTHPSFAEADLGLLDPAESVQRRLSAGGGSPKSVRDQISDLRRRIP
                     PS"
     gene            complement(2930..>3203)
                     /locus_tag="VFU96_09345"
     CDS             complement(2930..>3203)
                     /locus_tag="VFU96_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEU5113512.1"
                     /translation="QIVYNGFWFSPERTALQGLIDDIQQPVTGEARLVLYKGGAAVSG
                     RRSPNSLYDPGSATFEADDVYRQADAEGFISLNALRIRGFGGAGDG"
BASE COUNT          613 a         1041 c         1032 g          517 t
ORIGIN      
        1 tcgccgtgtc cccgagcgga cagctcgccg gctctgcgca agatctcgat ctcctcgcgc
       61 gatttgaaaa gacgcatctc tgcaaggagg acggtgatgt cacgcaccgc cgacggaacc
      121 gagcccccgt aacgctcgcg atgactcctg gccttggcca cgatccccgc gatccggtcg
      181 tcgtggtgcg ggttgccggt ccgataccag atgacctccc gcccgatcat ccgcctggtc
      241 aggatctcgt cgagctggcc cacctcatag gcgtgatccg ctccgaagcg ctccttggcg
      301 ccctcggtcc cggcgcgata gccattccat gcctcgagct ctgggtcgcg gggtctcacg
      361 aagagcgtgt agtcgccgtc cgcatggcca ggtgtgatga cggcgaccgc ttccggctca
      421 tggaagcctg tcagatatag gaaatccgag tcctggtgga actcgaactc ggtgtcgttg
      481 ctccgaatca cctctaccgc cgccggaatc acggcgatgg catcgtcacc gatctgctcg
      541 gccagacggg cgcgccgctc ggcataaggg tctgacatgc cgcgaacggt accgctgctg
      601 tctgccaggt cgatttcaga cttgcggatc aggtgcccgg agcgtgctcc aggtcgacga
      661 ccagcgggaa gtgatccgag cccagggcgg ggccgagtcg gcgatcgcga acccggagat
      721 cctcgctgtg aagcaggtga tcgatgggga cccgcaacgg aaagatcgtc atcgccgaga
      781 aagaaggctg aagaccgaac ccgatctggg agttgcgcag attgccctcc gagacgagcc
      841 cacgaaacgg cgaagaccaa ggcgaggcgt tcagatcacc caccacggcg taggcgccgt
      901 cttgctcggc tgcccatccg gcggcaaagg cgagctgggc gtcgcgaagg gccgcccgct
      961 cgcccgatgt cggcgccagc ggatgcgaag agaggatcgt caccggcacc ggccagccct
     1021 ctggctggta ctggagggag atggcccggc catcgccttc ggcaaagccg tacgagaccg
     1081 cctcgacctc atcacgagcc aacaccagag tcccgaatat gaggttgtcg gagcgacccc
     1141 ggacgatctc gtagtcgagc ccggacgatt ccatcgcaac ctcccagggc cggctcgact
     1201 catggagcag gaccaggtct ggatcggtcg cctcgatgta ttcgatgacc tcgccgtagc
     1261 tctcgttggt ggagagcaag ttgaagctca tcacccgcat tcctggcgct ccggccaccg
     1321 gatcgcccgg cgaaccgacg aatagcgggg caacgaagaa cagattgacg agggcgaccc
     1381 cgaggatggc gagcccggtg cgccgccagc ggctggcgac aacgatcagg ccgagcacag
     1441 ccaagatgat gacgtattgg gccctgaagt tggccatcac gtcgagccac caaacccaag
     1501 ccccggcaaa ggaggcgagg ctgatcaggg cgatgacggc cgcggggatc accacgagga
     1561 tcaccgggag cacgttagga aggagggatc ctcctccgca gatcggagat ctgatcacgc
     1621 accgatttcg ggcttccccc accggcactg aggcggcgct gcaccgattc ggccggatcc
     1681 aggagaccca gatcggcctc ggcgaacgac ggatgcgtag agatcaagtc gtccgccgtc
     1741 gcctcctgca gggtgcgacc ctgtccctcc aggacgagaa ccagcttgcc aactgcctcg
     1801 tgggcctcgc ggaacgggat gcctcgggcc acgagggcct cggacaggtc gaggctggcc
     1861 gtctccacat tcgggtccac cgggaggaac tcggtctgac ggagcatctc gaccatcgcg
     1921 gccagcgact gtgccagcgt gtcgtcggcc tcgaagacga gccgcttgtc ctcttggaga
     1981 tcacggttgt aggcgagagg aaggcccttc tggagcgtca tgatcgatgt cagcagtccc
     2041 gagacccgtg ctgcacgtcc ccgaaccagc tcggcgatgt ccgggtttcg cttgtgggga
     2101 agagcagacg agcccgtgct cacacggtcg cccagcttcg cccgattgaa ttcagaagta
     2161 gcccagataa tgacttcctc ggcgaggcgg gacaggttca ccatggcttg agcgcagcaa
     2221 aagacgtact ccgcgagatg gtcccgtgag ccgacggcgt cgagtgagtt ctcgaagacg
     2281 ccggacattc ccagctcgcc ggcggtcgca gcgatatcga tcggcagaga cgaacccgcc
     2341 gaagcgccgg cgcccaaagg tgacacgtcg atcctcgtgc tcactccggc gaatcggtct
     2401 gcgtcccgaa ccgccatcca ggcataggcg aggagatgat ggccgagagt cgtcggctgg
     2461 gcctgttgga gatgggtata agagggcacc acggtttccg cgtgacgttc ggcgatctcc
     2521 gccagcccca gggcgtaggc gtggagctgt ccgattcgct caccgcacac acgacgtgtg
     2581 tagagacgca tgtcgagagc gacctggtcg ttgcgagaac gcgcggtgtg cagcttgccg
     2641 gcggtgtccc caatcagttc gaacaatctg cgctccaccg ccgagtgaac gtcctcatcc
     2701 gactcggagt actcgaatac cccacccgcc gtctcggacc ggatcgtctc caatccgccg
     2761 acaaggatgt tcacctccaa ttcggtgagg atcccggact tcccgagcat gcggacatgg
     2821 gccagagacc cggtgatgtc gtcgtcgagg agacgtcggt cggagtcatc agttgtgaag
     2881 gtccacaaga cctggcccgg gcccgactcg aaacggccac cccagaggct catccgtccc
     2941 ccgctccccc gaagccccgg atccgcagag cattgagcga gatgaagcct tcggcgtcgg
     3001 cctggcggta gacgtcgtcg gcctcgaagg tggccgatcc agggtcatag aggctgttgg
     3061 gtgagcgccg ccccgagacg gcggcaccgc ccttgtacaa caccagccgc gcctcaccgg
     3121 tgaccggctg ctggatgtcg tcgatgagac cctgcagcgc agtccgctcc gggctgaacc
     3181 agaagccgtt gtagacgatc tgg
//
LOCUS       DASTXO010000444         3077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_173451, whole genome shotgun sequence.
ACCESSION   DASTXO010000444 DASTXO010000000
VERSION     DASTXO010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173451"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="VFU96_09350"
     CDS             complement(<1..1233)
                     /locus_tag="VFU96_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5113513.1"
                     /translation="MTTAPAPVPIRSTIPARLDRLKWTRFHTRMVFGLGAAWILDGLQ
                     ITIASSVTGVLTQPDTLGMTSTQIGLIASVYLVGQLIGALYFGKRSDALGRKRLLIYT
                     LLLYLLGTGAAAFVTGHHTGWLVFFYATRLVAGMGIGGQYAAINSAIDEMMPSQYRGR
                     VDIWINGSYWAGAILGSFAALFFLNQFPPNEGWRLAFLMGPVLALVVIIVARTLPESP
                     RWLLTHGRVEEAEENVRKIEEVAVQMGQELEPVDDSKALELVPEEPGGYGEFLRLAFR
                     SYRPRAILGASLMITQSFLYNAIFFTYALVLTTFYGVSATAVPLYGLAFSVGNLLGPL
                     ILGPLFDSVGRKKMISGTYILSGALLAISAFLFQAGVLDATTQTVMWVITFFFASAGA
                     SAAYLTVSETWPVEVRAKA"
     gene            complement(1278..2045)
                     /locus_tag="VFU96_09355"
     CDS             complement(1278..2045)
                     /locus_tag="VFU96_09355"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006342072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEU5113514.1"
                     /translation="MSLKDKVAIVTGGNSGIGKAIVLALAEEGANIVIDFVAHPEATD
                     ELEKQIHALGDKAIGVEADVSKVADLQRLMDTAVEKMGRVDIMVNNAGIETRTSVLDT
                     TEEQYDRVLDINLKSAFFGTQIAAKQMIAQGDGGRIINISSVHEDWPMPGNTAYCLSK
                     GGMRMLTRTAAQELGPHNVLIVGVGPGAVATPINLGTMEDPGKMKVLDAAIPLGRMAQ
                     PEEIGSVVAFLAGDGATYMTGTTVFADGGLMHSSPGL"
     gene            complement(2062..>3077)
                     /locus_tag="VFU96_09360"
     CDS             complement(2062..>3077)
                     /locus_tag="VFU96_09360"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5113515.1"
                     /translation="GTKKLYVFGLLANLVAMSLLVVSQFFTGQESTAYALLLLATASL
                     GVGFGLTVPALNTFTAAFNPSKVDASVLVLNALLGLGTALAPVFVALFVGLGFWWGLP
                     VLSGALLVLLLLNSLRLPLETAAPAGGTTGRTTVPPRFWWYAAFAVTYGICETMNGNW
                     SSLAMQDLGASATQASLALTTFWAAVTIGRVGLAMIERRFPERRAYRLLPFLLTAVFL
                     AISALPSDQPGLGILVFGLAGLGCSALLPLTISFGQGELVAMSGAVAGGVIAFYQFGY
                     GIAAFGAGPLEESGIELSTLFGYTAVVALVMGVIALTITGRQTTSPRSVPSNGAGRLA
                     NGH"
BASE COUNT          602 a          941 c         1005 g          529 t
ORIGIN      
        1 cgccttggcc cgcacctcca cgggccatgt ctcgctcacg gtgagatacg ccgcactggc
       61 tccggccgaa gcgaagaaga aggtgatcac ccacatcacg gtctgggtcg tcgcatcgag
      121 cacacctgcc tggaagagga atgcactgat tgccaggagg gctccggaaa ggatgtatgt
      181 cccggagatc atcttcttcc gaccaaccga gtcgaaaagc gggccgagga tcagtggacc
      241 caacaggttg cccaccgaga aggcgaggcc gtagagcggc accgcagtcg cacttacgcc
      301 atagaaggtg gtcagcacca gcgcataggt gaagaagatg gcgttgtaga ggaacgactg
      361 ggtgatcatc agagaagcgc caaggattgc tcgcggccgg taagagcgga acgccagtcg
      421 gaggaattcg ccgtagccac cgggttcctc cggcacgagc tcgagggctt tgctgtcgtc
      481 cacgggctcc agttcctggc ccatttggac cgccacctct tcgatcttcc tcacgttctc
      541 ctcggcctct tcgaccctcc cgtgggtgag cagccaacga gggctctccg ggagggtgcg
      601 cgccacgatg atgactacga gagcgagcac cgggcccatc aggaaagcca gtcgccaccc
      661 ctcatttggt gggaactgat tgaggaagaa gagggcggca aaggagccca agatggcccc
      721 ggcccagtag ctgccgttga tccagatatc gacccggcct cgatactgcg agggcatcat
      781 ctcgtcgatc gccgagttga tggcggcgta ctgcccgccg atccccatcc cggctaccaa
      841 tctcgttgcg tagaagaaga ccagccatcc ggtgtgatgg ccggtgacga acgctgcggc
      901 tccggtcccg agcaagtaga ggagaagcgt gtagatgagc agacgtttgc gaccaagagc
      961 gtccgaccgt ttcccgaagt agagagctcc gatcaactga ccgaccaggt acaccgaggc
     1021 gatgaggccg atctgggtcg aggtcatccc cagcgtgtcc ggctgggtca ggactccggt
     1081 gaccgagctg gcgatggtga tctggagacc atcgaggatc caagcggcac cgagcccaaa
     1141 caccatcctc gtgtggaaac gggtccactt cagtcggtcg agtcgtgccg ggatggtgct
     1201 acgaatcgga acaggtgctg gcgcagtggt cactgatctc ctccttcact catcccgcta
     1261 cggacggacg gatcccctca cagacctggg ctggagtgca tgaggcctcc gtcggcgaac
     1321 acggtggttc cagtcatgta cgtcgcgccg tctccggcga ggaaggcaac gacgcttcca
     1381 atctcctctg gttgggccat gcgaccgaga gggatggcgg cgtcgagcac tttcattttc
     1441 cccgggtcct ccatcgttcc gaggttgatc ggagtcgcca ccgcaccggg cccgacgccg
     1501 acgatcagaa cattgtgcgg acccagctcc tgggcagcgg tgcgggtgag catcctcatc
     1561 cctcccttgg acaggcagta ggccgtgttc ccgggcatgg gccagtcctc gtgaaccgag
     1621 ctgatgttga tgattcggcc cccatcgccc tgtgcgatca tttgcttggc ggcgatctga
     1681 gtcccgaaga aggcgctctt caggttgatg tcgagaacgc ggtcgtactg ctcctcggtg
     1741 gtatcgagca ccgaggtccg cgtctcgatc ccggcgttgt tgaccatgat gtcgacccgc
     1801 cccatcttct ctacggcggt gtccatcagt cgttgcaggt cggcgacctt gctcacgtcg
     1861 gcctccacgc cgatcgcctt gtctcccagg gcgtggatct gcttctccag ctcgtcggtg
     1921 gcctcggggt gggccacgaa gtcgatgact atgttcgccc cttcctcggc cagtgccagc
     1981 acgatggctt ttccgatccc gctgttgccc ccggtgacga ttgcgacctt gtctttgaga
     2041 ctcatgggat tgctccttgt gtcagtgccc gttggcgaga cgtcccgcac cgttggacgg
     2101 gacgcttcga gggctggtgg tttgacggcc cgtgatggtg agtgcgatca cccccatcac
     2161 aagggcgacg acggcggtgt atccgaacaa tgtcgacagc tcgatacccg actcctcgag
     2221 aggaccggct ccgaaagcgg cgatcccgta gccgaactgg tagaaggcga tcactccgcc
     2281 ggccactgcg ccggacatcg cgaccagctc gccttgaccg aagctgatcg tgagcgggag
     2341 gagcgccgag cagccgagcc cggccaggcc aaagaccagg atgccgagac ctggctgatc
     2401 gctggggaga gcggagatag ccaggaagac cgcggtcagc aggaacggca ggaggcgata
     2461 agcgcggcgc tcggggaagc gacgctcgat catggccaag cccactctgc cgatggtcac
     2521 cgccgcccag aacgtggtga gggccagaga ggcctgggtc gccgaggccc cgagatcctg
     2581 catggcgagg gacgaccagt tgccgttcat ggtctcacat atcccgtagg tgaccgcgaa
     2641 cgcggcatac caccagaaac gtggcgggac ggtcgtcctg ccggtggttc cccctgcggg
     2701 cgcagccgtc tcgagcggga gacggagact gttcaggagg agcaaaacga ggagagctcc
     2761 ggagaggacg gggaggcccc accagaatcc gagaccgacg aacagggcga cgaagaccgg
     2821 ggcaagtgcg gtgccgaggc cgagcagtgc attcaggacg aggaccgacg cgtcgacttt
     2881 gctcggattg aacgctgcag tgaaggtgtt gagagcgggc accgtgagcc caaacccgac
     2941 tccgaggctg gcagtggcga ggaggagcag tgcataggcg gtggactcct ggcccgtgaa
     3001 gaattgactc accaccagca aggacatcgc caccaggttg gccaagaggc cgaagacgta
     3061 cagcttcttg gtgccga
//
LOCUS       DASTXO010000445         3232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_542626, whole genome shotgun sequence.
ACCESSION   DASTXO010000445 DASTXO010000000
VERSION     DASTXO010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542626"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="VFU96_09365"
     CDS             <1..1122
                     /locus_tag="VFU96_09365"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU5113516.1"
                     /translation="DDSDADHSAEGVDAESRSKPGQADADYEGMSFPQDLDDFEANQF
                     TGSTTQEYQGLAEEVNRAAGEEWEQQAVAATVPGVDSGLVGFEDVTGARGEAEEDFEA
                     SEQAATSDLAMRVASAVVIFGLFLGSLLLGGWWFAAFVILAMIVSAGEFYATARSEDS
                     RPLALFGLLGVVFMGVGAQLTGAAAIAAWAAAFSVVTILFLTLSPRRQPLEDASVTSS
                     GMVWVGVLSFAILIAQGPHPVAYILFVVLLVAANDTGAYFVGRAFGRHKLSPMISPEK
                     TVEGLVGGLIAGLVIASILATFPAWETIGIAKALVAGAVVGLIAPLGDAVESMVKRSL
                     GVKDMGSVLPGHGGMLDRIDGFLFAVPAIYFLFRGFGLL"
     gene            1119..2264
                     /gene="dxr"
                     /locus_tag="VFU96_09370"
     CDS             1119..2264
                     /gene="dxr"
                     /locus_tag="VFU96_09370"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEU5113517.1"
                     /translation="MTRPVVVLGATGSIGSQTIEVAASLGLDIAALAARSPSAKLAEL
                     AMAHPTAQVMVAGGASDERAEFVAMVGRPVQFGADAVIDAARTPGTIVVNGIVGAAGL
                     RATIAGLESGNRVALANKESLVAGGPVVKRALAEHGGELIPVDSEHSALLQCLAGEEA
                     DSVSRLILTASGGPFRGRSLDSLAGVTPEEALAHPTWEMGPRITIDSATLFNKGLEVI
                     EAHHLFGVDYDRITVLIHPQSILHSAVEFVDGSWKGHLGHPDMRIPIQYALTSPDRAP
                     SLARPFDLAGMDLTFEAPDPVSFPALDLAFEAGREGGSSPAVLNAADEVAVAAFLDGR
                     LGFLGIAEVVSATLETADWRSLDSVEDVVAADREGREIAASFIAGAC"
     gene            2295..>3232
                     /locus_tag="VFU96_09375"
     CDS             2295..>3232
                     /locus_tag="VFU96_09375"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU5113518.1"
                     /translation="MLGGFAAAAAIILFVTAHEAGHFFAAKATGMKVTEFFFGFGPRI
                     FSWRRGETEYGIKAVPLGGYVRIIGMNPLEEVSPADMGRTYREKKFWEKSVVVLAGVG
                     MNFLIAFLLFYGIVLANGVSEPQPVVREVVEEAEVGEDTVDTPAMVAGLEQGDVIISI
                     GDHRVDEWSDVPEALTAVGAGPTVVAVDRDGQLINIDVVLIDADGVEGGAGFLGVSPV
                     IETHDPGVIGAVGVAGSQVWEGIGFTFQAIGDMIKPSSLAQYLGVLAGDTDVDEEIRP
                     VSPIGIVNIGTQTESAAAFLGILAFVNVILATINLLP"
BASE COUNT          501 a         1033 c         1089 g          609 t
ORIGIN      
        1 gatgactccg atgccgacca ctcagcggag ggggtggacg ctgagtcgag gtccaaaccg
       61 ggacaggcag acgccgacta cgaggggatg agcttccccc aggatctcga cgacttcgag
      121 gcaaaccagt tcaccggttc caccacccag gagtaccaag gtttggccga ggaggtgaat
      181 cgggccgcag gagaggagtg ggagcaacag gccgtcgcgg cgactgttcc cggggtggac
      241 agcgggttgg tcgggttcga ggacgtcacc ggcgcccgcg gcgaagccga ggaggatttc
      301 gaagcttcgg agcaggcagc caccagcgac ctggcgatga gggtcgcttc tgccgtcgtc
      361 atcttcgggt tgttcctggg cagtctcctg ctcggtggct ggtggttcgc agccttcgtg
      421 atcctcgcca tgatcgtgtc cgcgggtgag ttctacgcca cggcccgatc cgaagacagt
      481 cgccctctgg cgctgttcgg gctgctcggc gtggtcttca tgggggtggg cgcccaactg
      541 accggcgccg ccgccatagc cgcctgggca gccgcgttct cggtcgtcac cattctcttc
      601 ctgactctgt caccccggcg ccaacccctg gaagatgcgt cggtcacgag cagcggcatg
      661 gtctgggtcg gagtgctctc cttcgccatc ctgatcgcgc agggacccca tccggtcgcc
      721 tacatcctct tcgtcgtcct actcgttgcc gccaatgaca ccggcgccta cttcgttggc
      781 cgcgctttcg gccgacacaa gctgtcaccg atgatctcac cagagaagac cgtggaggga
      841 ctggtgggtg gcctcatcgc cgggctggtg atcgcctcga tcctcgccac gtttcccgcc
      901 tgggagacca ttggcatcgc caaggcattg gtggcgggcg cagtcgtcgg gctgatcgcg
      961 ccactcggcg acgccgtcga gtcgatggtc aagcgctcgc tgggtgtcaa agacatggga
     1021 tcggtcctcc ccggccacgg tggcatgctc gaccgaatcg acggtttcct cttcgcggtc
     1081 cctgccatct acttcctgtt ccgcgggttc ggtttgttgt gacccgcccg gtggtcgttc
     1141 tcggggcgac cgggtccatc gggtcgcaga caatcgaggt ggccgcgtcg cttggcctgg
     1201 atatcgcggc cctcgctgcc cggtcgccat cggcgaagct ggccgagctg gcgatggccc
     1261 accccaccgc tcaggtcatg gtcgcaggcg gcgcctccga tgagcgtgcg gaattcgtcg
     1321 ccatggtggg gcgaccggtc cagttcggcg ccgatgccgt catcgacgca gcccgcaccc
     1381 caggcaccat cgtggtcaac ggcatcgtgg gagcagccgg gctgagggcg acaatcgccg
     1441 gcctggagag cgggaatcgc gtcgcgctgg ccaacaagga gagcctggtg gccggggggc
     1501 cggtggtgaa gcgagccctg gccgagcacg gcggcgagct gatccctgtc gacagcgagc
     1561 attctgccct cctccagtgt ctcgccggag aagaggcgga ttcggtgtcg cggctgatcc
     1621 tgaccgcgtc cggtggcccg tttcgtggca gatccctgga ttcgctggcc ggggtcactc
     1681 ccgaagaggc actcgcccac cccacgtggg agatgggacc ccggatcacc atcgactcgg
     1741 caaccctgtt caacaagggc ctggaggtga tcgaggccca tcacctgttc ggcgtcgatt
     1801 acgaccgaat cacggtattg atccaccccc agtcgatcct ccactccgcg gtcgagttcg
     1861 tcgacggctc ctggaagggc catctgggtc atcccgacat gaggatcccg atccagtacg
     1921 cattgacctc cccggatcgg gcgccgtctc tggcccgccc cttcgatctg gccgggatgg
     1981 atctcacgtt cgaagcgccg gatcccgtgt cgttcccggc cctggacttg gctttcgagg
     2041 cggggcgtga aggtgggtcc tcgccggcgg tcctcaacgc ggcagatgag gtggcggttg
     2101 cggcgtttct cgatggccgc ctcggcttcc tcgggatcgc cgaggtcgtc tcggcgactc
     2161 tcgagaccgc cgactggcga tcgctcgatt cggtggagga tgtggtagcc gccgaccgtg
     2221 agggccggga gattgctgca tcgttcatcg ccggagcctg ctgactatcg acgattcgcg
     2281 ggtagcgtgt ccgcatgctc ggaggctttg ctgcggccgc agccatcatt ctcttcgtca
     2341 ctgcccacga ggcaggtcac ttcttcgcgg ccaaggcgac ggggatgaag gtgaccgagt
     2401 tcttcttcgg ctttgggccc cggatcttct cgtggcggcg cggggagacc gaatacggca
     2461 tcaaggcagt cccgctcggc ggctatgtcc ggatcatcgg catgaacccc ctcgaggagg
     2521 tgtcacccgc cgacatggga cgcacgtacc gggagaagaa gttctgggag aagagcgtgg
     2581 tcgtgttggc cggggtgggc atgaacttcc tcatcgcatt tctcctcttc tacgggatcg
     2641 tgctcgccaa cggggtctct gagccgcaac ccgtggtcag ggaggtcgtc gaagaggcgg
     2701 aagtcgggga ggacaccgtt gacacgccgg caatggttgc cgggctcgaa cagggcgatg
     2761 tcatcatttc catcggggac cacagggtcg atgaatggtc cgacgtcccc gaggctttga
     2821 ccgccgttgg cgccggcccg acggtcgttg ccgtcgaccg tgacggccaa ctcatcaaca
     2881 tcgacgtggt cttgatcgac gccgatggcg tcgagggtgg agcgggattc ctcggcgtga
     2941 gcccggtgat cgagacacac gacccggggg tcatcggtgc cgtgggtgtg gccggaagcc
     3001 aggtatggga ggggatcggg ttcacctttc aggcgatcgg cgacatgatc aagccatcga
     3061 gcctcgccca gtacttggga gttctcgccg gcgacaccga tgtcgacgag gagatccggc
     3121 cggtgagccc aatcggcatc gtcaacatcg gcacccaaac cgagtcggcc gccgcatttc
     3181 tcggcatcct cgctttcgtc aacgtgatcc tggccaccat caacctcctc cc
//
LOCUS       DASTXO010000446         7820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1020630, whole genome shotgun sequence.
ACCESSION   DASTXO010000446 DASTXO010000000
VERSION     DASTXO010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7820
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020630"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>7820
                     /locus_tag="VFU96_09380"
     CDS             <1..>7820
                     /locus_tag="VFU96_09380"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEU5113519.1"
                     /translation="SYRELSKAIREEALLTPAAEWLVDNFHLVESQLHEIRENLPSSY
                     YRELPKLDGGHLAGYPRVYGIVWAYVSHLDSRFDPEALRRFVASYQEVSPLTIGELWA
                     IPITLRLILIENLRRMAESIIWARRLRREAEEIADGLLGVGGGRPGESQAVLDRYGPA
                     KLPQPFAVQLLQRLRDQDPETTPGLTWLLDRLSAQGTSPDEIVGETHRRQAATNVTVR
                     NIVTSLRQVNFFDWADFVEGVSLVDHALAAESSYGEMSFATRDRYRHAIEELSKGSGT
                     SELDVTKTTLRLADPQLADRRESDPGFYLLGEGRTTLEAEIGFRPGVGLVASRFVRRH
                     AAAVYLSSVAVVTALILALPLLTVEQWSPWGWVMAGLALIPASDMGVILVNRAIAALT
                     RPEELPSLALAHGPTADLRTLVAIPAMLTRPDQVEELVERLEVHYLANPDGDLHFALV
                     SDWRDAEGPELADDAELLAAAADGVLRLNERHGPGPGGADRFLLFHRRRLFNPGEELH
                     MGWERKRGKLHELNRLLRGASDTSFIPSDRTPTGVRFVIALDADARLPPGAATRLVAT
                     IAHPLNKARVDESLGRVVEGYGVLQPRVSAFLRASGISYFQRVFFPPGGIDPYAAAVS
                     DLYQDLFGEGSYAGKGIYEIDAFEAALEGRIPENALLSHDLFEGSFARAGLVSNVEVF
                     EEFPSHYEVALSRQHRWVRGDWQLLPWILGSHGPLPPVARLKMVDNLRRSLSGPAALF
                     TLIASFILPGVPVWPWTAFILVALALPGMLPLLERISPRRTSTPGAVRLRRWGTDASR
                     ALGRTLLQAAFLAHQALRMTEAIVVTLWRLVFTRRGLLTWVTAEEAERQFDLELTGFI
                     RRMSPSMVVGIAGSTAAAVVKPEALVAALAWSGMWVLAPLIAHLVSTPRPAEVADPPT
                     LQEQEALRLIARRTWRYFERFVTSDENWLPPDNFQEDLSQIAHRTSPTNVGLYLLAVL
                     SAREFGWIGLEETVTRLENTVTTLNRLERYQGHFLNWYDTTSCEPLEPRYVSTVDSGN
                     LAGHLLAVAQGCERLRELATGPEVAAGIRDSALLLREAADPVAFDAGIEVVSPRELDR
                     ALVEFLDSLRSRGAQGSDRLAEILAEAETVADIARTMATREGAGDLAVSAEALLATVR
                     GHLHDQQAADDAISRELLHSRLSALAVATAGLAADMNFSFLMNRDNKLLAVGYRVGEN
                     ALDQSHYDLLASEARLASFVGIAKGDLPPSHWFRLGRSMTPMGVESALISWSGSMFEY
                     LMPLLVMRSPPESILETTYRAVVRRQIEYGTERRIPWGISEAAYAARDIHLVYQYRAF
                     GVPGLGFERGLSEDLVVAPYATLLATMVEPGDAVTNLVRLNSLGAQGRYGYYESLDFT
                     PSRLPEDGESVLVRAYMAHHQAMSLISVTNVIFAGLCRDLFHSDPWVRAAELLLHERV
                     PRDVEVARPRAEEVAESAHPLEEAKGVVRRISSPHGPGPATHALSNGRLSTIITAAGS
                     GYSQWQGLAVNRWREDATRDNFGSYFFIRDGAGRTWSAGYQPALAEPDSYQVAFSEDK
                     AEISRRDGNVSTTLTVVVSAEDDAEVRRVSLTNQGSVAREVEVTSYAEIVLASARADA
                     AHPAFSNLFVQTEVVTGRDALLASRRPRSDEEETVWAGHVLAVTGEVVGGIQYETDRA
                     RFLGRGNQIHHAAAVDRPLSNTAGRVLDPVFSLRRKVRIEPGATAHLDFTTVVARTRD
                     EALDLVDKFSETAAFERISTLAWTQAQVRLHHLRIEPDQAHIFQQLAGSLIYFDPALR
                     APPAPNVTSSVSRDGLWRHGISGDFPIVLVRIDRPEERGLVRDLLRAQEYWRTKGLVA
                     DLVIINEEAGTYAQELHRAIEELIRESGHLVGQEPDGGVFVLNGPLLTIEDRTTLHAA
                     ARAVLLSHHGTLADQLARRPTNELAPTTRAKASAPDLTGGSHPRPDREFHNGLGGFAA
                     DGSEYVVVLGEGQSTPAPWINVVANRRFGFCVSESGSGYSWSENSRENQLTPWSNDPV
                     SDRPGEVLYVRDEESGEVWGPTALPIRDDVFGYLARHGPGYSEFEHRSRGIALHLHQT
                     VPLEDPIKISRLRIRNLTGRRRRLSVTAYVEWVLGTTRAVSPMQILTERDEETGAVLA
                     RNPWNVDFGDRVAFADLGEGEVTITGNRAEFLGRYGSVEDPAALRVDGKLSGTVGVGL
                     DPCAAIQRSLTLAPNEEAEVVFLLGQGKDEAEARTLVQRYRRTDPEEVKAEVATRWND
                     VLGNIRVKTPDRSFDLMINQWLLYQTLACRVLARTAFYQSGGAFGFRDQLQDVMALVT
                     PMRDTAREHLLRAAAHQFEEGDVLHWWHEPSGKGVRTRISDDYLWLPYAVAHYVEVTG
                     DQDIFEEVVPYLRGMELAPDQDETFFTPERSSIEGTVFDHCRRALDRALSRLGSRGLP
                     LIGTGDWNDGFNRVGREGRGESVWLGWFLEANLSRFADIADRSSETELAATWRRGALR
                     LRASLEAEAWDGDWYRRAFFDDGTPLGSSDNPECRIDSIAQSWSVIHGGAPADRAQRS
                     MDSVEEYLVHRGDGLVLLFTPPFDDWEVDPGYIKGYLPGVRENGGQYTHAAIWAAMAM
                     IEAGRVEEGWR"
BASE COUNT         1311 a         2529 c         2579 g         1401 t
ORIGIN      
        1 tcataccggg agttgtccaa ggcaatccgg gaagaggcgc tgcttacccc tgcggcggaa
       61 tggctggtgg acaacttcca tctcgtggag agccagctac acgagatacg ggaaaacctc
      121 ccttcgagtt actaccgcga gcttcccaag ctcgacggtg gtcatttggc cggctacccg
      181 cgggtttacg ggatcgtgtg ggcatacgtc tcgcacctgg atagtcgctt cgatcccgaa
      241 gcgctgcgca gattcgtggc ttcataccag gaggtctcgc ccctgaccat cggtgagctg
      301 tgggccatcc ccatcaccct tcgcctgatc ctgatcgaga acctgcgccg gatggcggag
      361 tcgatcattt gggccaggag gcttcgtcga gaggccgagg agatcgccga cggacttctc
      421 ggcgtcggcg gcggccgtcc cggcgagagt caggcagtcc tcgaccgcta cggccccgcc
      481 aagctgccac agccctttgc cgtccagctg ctgcagcgtc ttcgcgacca ggacccggag
      541 acgaccccgg gcctcacctg gctgctggat cgtctcagcg cccaggggac gtcacccgac
      601 gagatagtcg gcgaaaccca tcgtcggcaa gcggccacca acgtcacggt acgcaacatc
      661 gttaccagcc tccgtcaggt caacttcttc gactgggccg acttcgtgga gggcgtcagc
      721 ctcgtcgatc atgccttggc cgcggagagc tcctacggcg agatgagctt cgccacccgg
      781 gatcgttacc ggcacgccat cgaagagctg tcgaagggat ccgggacctc cgagctcgat
      841 gtgacgaaga caacacttcg actggccgac ccccaactcg ccgatcgtcg cgagagcgat
      901 cccggcttct acctgcttgg agagggcagg accaccctcg aggctgagat cggcttccgg
      961 cccggtgtcg ggctggttgc gtctcgattc gtcaggaggc acgcggccgc cgtgtacctg
     1021 agctcggttg ccgtggtcac cgctctaatc ctggccttgc ccctgctcac cgtcgagcag
     1081 tggtctccat ggggatgggt gatggccggc cttgccctga tcccggcttc cgatatgggc
     1141 gtgatcctcg tcaaccgcgc cattgcggcc ctgacccggc ccgaggagct gccttcactg
     1201 gcgctggctc atggaccgac cgcagacctg cgcacgttgg ttgcgatccc cgccatgttg
     1261 acgcgacccg atcaggtcga ggagctggtt gaacggctcg aggtccatta cctggccaat
     1321 ccggatggcg atttgcactt cgcgctggtg agcgactggc gagacgccga agggcccgag
     1381 ctggccgacg acgccgagct cttggctgcg gcggccgacg gcgtgctccg actcaatgag
     1441 aggcacggac cagggccggg aggggcggat cgattcctgc tcttccatcg ccggcgtttg
     1501 ttcaatcccg gcgaggaact ccacatgggc tgggagcgca agcggggcaa gctacacgag
     1561 ctcaaccgtc ttctccgggg agcctccgac accagcttca tcccatccga ccggacgccg
     1621 acaggcgtcc gctttgtgat cgctctcgac gccgacgccc ggctcccccc gggggctgcg
     1681 acgaggctgg tggcaacgat tgcccatcca ctcaacaagg cccgcgtcga tgaatcactc
     1741 gggagggtgg tggagggata tggagtcttg caaccgcgag tctccgcctt cctgagagcg
     1801 agcggtatca gctatttcca gcgtgtcttc ttcccgcctg gcgggatcga cccctatgcc
     1861 gctgccgtca gtgacctgta ccaggacctg tttggcgaag ggtcctacgc cggcaagggg
     1921 atctatgaga tagatgcctt cgaggcggcg ttggagggtc gaatccccga gaacgccctg
     1981 ctcagccacg acctcttcga aggaagcttc gcccgggccg gtctggtgag caacgtcgag
     2041 gtgttcgaag agttcccgag tcactacgag gtggccctgt cacgccagca tcgctgggtc
     2101 cgtggcgact ggcagctcct cccttggatc ctcggcagtc acggaccgct gcctcccgtg
     2161 gctcgtctca agatggtgga caacctccgc cgcagtctca gcggaccggc tgccctgttc
     2221 accctgatcg catcgttcat tctgcccggg gtcccggtct ggccatggac cgcgttcatc
     2281 ctcgtcgcgt tggcccttcc cgggatgctg cccctgctcg agcggatcag tccgcgccgg
     2341 accagcacgc cgggggcggt gcggctgaga cgttggggaa cggatgcatc gagggcactg
     2401 ggacggaccc ttctgcaggc cgcattcctc gcccaccagg cgttgaggat gaccgaagcc
     2461 atcgtggtga ccctttggcg cctggtattc actcgtcggg gattgctcac gtgggttacg
     2521 gccgaggaag cggagcggca gttcgacctg gagctgacgg gcttcatccg gcggatgtcc
     2581 ccctcgatgg tggtcgggat tgcgggcagc accgcggccg ctgttgtcaa acctgaggcg
     2641 ttggtggcag ctctggcctg gtccgggatg tgggtgctgg ctcccctcat cgcccacctg
     2701 gtcagcaccc cacgccctgc cgaggtagcg gacccaccaa cgcttcagga acaggaggcg
     2761 ctccggctga tcgcccggcg gacctggcgc tatttcgaga gattcgtgac cagcgacgag
     2821 aactggctgc cacccgacaa tttccaggag gacttgtcac agatcgctca ccgcacctct
     2881 cctaccaatg tcgggctcta tctgctggcg gtgctctcgg cccgggagtt cggctggatt
     2941 gggctcgaag agacggtgac caggctcgaa aacacggtca ccacgctcaa tcggctggag
     3001 cgatatcagg gtcatttcct caactggtac gacacgacct cgtgtgagcc cttggagccc
     3061 agatacgtgt cgaccgtgga cagcggcaac ctggccggcc acctcctcgc ggtagcccag
     3121 ggctgtgaac gccttcgcga gctcgccaca ggcccggagg tggcagccgg tatccgcgac
     3181 agcgccctgc tgttgcggga ggcggccgac cccgtcgcat tcgatgcggg catcgaggtc
     3241 gtgtcgcccc gggagctcga tcgagccctg gtcgagtttc tcgactcgct gcggagcaga
     3301 ggagcccagg ggtcggatcg tctcgccgag atcctcgccg aagccgagac cgtggccgac
     3361 atcgcacgca ccatggcgac gagggagggt gccggggact tggcggtctc ggccgaagcc
     3421 ttgttggcga cggttcgggg tcaccttcac gaccagcagg cagccgacga tgcgatcagc
     3481 cgggagcttc ttcacagtcg tctgtccgcc ctcgccgttg cgaccgccgg gctggcggct
     3541 gacatgaatt tctcgtttct gatgaaccgt gacaacaagc tgctggcagt gggctatcgc
     3601 gtcggcgaga acgccctcga ccaaagtcat tacgacctgc tggcctctga ggcccgcctg
     3661 gccagcttcg tgggcatcgc caaaggagac cttcccccca gccattggtt ccgactgggc
     3721 cggtcgatga ctcccatggg tgtcgagtcc gccctgatct cgtggtcggg ctcgatgttc
     3781 gaatacctga tgccactgtt ggtgatgcgc tcgccacccg agagcatcct cgaaaccacc
     3841 taccgggcgg tcgtgcgacg ccagatcgag tacgggaccg agcgtcggat accgtggggg
     3901 atctccgagg cggcctacgc cgctcgggac atccacctcg tctatcagta ccgggcgttt
     3961 ggggtcccgg ggctcggctt cgaacggggt ctcagcgagg acctggtggt ggcgccgtat
     4021 gcaaccttgc tggcgaccat ggtcgagccg ggcgacgcgg tgacgaacct ggtccggctc
     4081 aacagtctgg gagcgcaggg caggtacggg tactacgagt cgctcgactt cacacccagc
     4141 cggctgcccg aagacgggga gtccgtcctg gtgcgagcct atatggccca ccatcaggcg
     4201 atgtccctga tctccgtgac caacgtcatc tttgccgggc tctgcaggga cctctttcat
     4261 tccgacccgt gggtccgggc ggccgagctg ttgttgcatg aaagggttcc tcgcgatgtg
     4321 gaggtggccc gtccccgtgc cgaggaggtc gccgaatcgg cccatcccct cgaagaggcg
     4381 aagggtgtgg tccgtcggat ctcgtcgcca cacggcccgg gcccggccac tcatgccctg
     4441 tccaacggcc gtctcagcac catcatcacc gccgccggtt ctggttacag ccagtggcag
     4501 gggctggccg tcaaccgctg gcgggaagac gccactcgcg acaacttcgg cagctacttc
     4561 ttcatcaggg acggggcggg gcgcacctgg tcggccgggt atcaaccggc tctggccgag
     4621 cccgactcct atcaggtggc cttcagcgaa gacaaggcag agatctcgcg tcgtgacggc
     4681 aacgtctcca ccacgctgac tgtggtggtg tccgcagagg acgacgccga ggtccgtcga
     4741 gtgagcctca caaatcaggg ctcggtggcc agagaagtgg aggtcacctc ctacgccgag
     4801 atcgtcctgg catcggcccg ggccgatgcc gcccaccctg ctttctccaa tctcttcgta
     4861 caaaccgagg tggtgaccgg acgcgacgcg ctgctcgcca gccgccgacc gcgctcggac
     4921 gaggaggaga cggtgtgggc aggccatgtc ctcgcagtca ccggcgaggt agtcggtggg
     4981 attcaatatg aaacggatcg ggcccggttt ctcggccgcg gaaaccagat ccaccacgcc
     5041 gccgcagtcg atcgtcccct gtcgaacacc gcgggccggg tgctcgatcc ggtcttcagt
     5101 ttgcggagga aggtgcggat cgagccgggc gccaccgctc acctcgactt caccaccgtg
     5161 gtggctcgca cccgggacga ggccctcgat cttgtcgaca agttctccga gacggcggcc
     5221 ttcgagcgaa tctcgacact ggcctggact caggcccaag tccgtctcca tcaccttcgc
     5281 atcgagccgg atcaggccca catcttccaa cagctcgccg gatcattgat ctacttcgac
     5341 ccggcgctga gagccccacc ggctccgaac gtcacgtcct cggtgagccg ggatggattg
     5401 tggcgacacg gtatctctgg tgacttcccg atcgtcctgg tccgaatcga ccggcccgag
     5461 gagcgtggac tggtccggga cctgcttcga gcccaggagt actggcggac caaaggcctg
     5521 gtagccgacc tggtgatcat caacgaggaa gccggcacgt acgcccagga gttgcaccgg
     5581 gccatcgaag agctgatcag agagagtggg catctggtgg gtcaggagcc cgacggcggg
     5641 gtcttcgttc tcaatgggcc gctgctcacc atcgaggatc ggacgaccct ccatgcggcg
     5701 gcccgggcgg tgctgctcag tcaccacggg acgctcgccg accagctggc ccgtcggccg
     5761 accaacgagc tagccccgac cacccgagcc aaggcgtctg cccccgacct gacgggcggt
     5821 tcgcatccga gaccggaccg cgagttccac aacggtctcg ggggtttcgc cgccgacggc
     5881 agcgagtacg tggtggtgtt gggagagggc caatcgacgc cggccccttg gatcaatgtc
     5941 gtcgccaacc gtcggttcgg gttctgtgtc tcggaatccg gctccggata cagctggtcg
     6001 gagaacagcc gggagaacca gctgactccc tggtcgaatg atcctgtctc cgaccgtccc
     6061 ggcgaggtgc tctatgttcg agacgaggag agtggggagg tatgggggcc aaccgccctg
     6121 ccgatcaggg atgacgtctt cggttacctg gctcggcacg gtccgggtta ctccgagttc
     6181 gagcacaggt cccggggaat tgccctccac ctccatcaga ccgtgccgct cgaggatccg
     6241 atcaagatct cgcgtctgcg tattcgcaac ctgaccggcc gaagacgccg cctgtccgtc
     6301 accgcctatg tggagtgggt gctgggaacg accagagccg tttctccgat gcagatcttg
     6361 accgaacgcg acgaggagac cggcgccgtc ctcgcccgca acccctggaa cgtcgacttc
     6421 ggggatcggg tggcctttgc cgatctcggg gagggcgagg tgacgatcac cgggaatcgg
     6481 gccgagttcc tgggtcggta tggcagtgtc gaggacccgg ccgcactgag ggtcgatggg
     6541 aaactgtcgg gaacggtcgg agtggggctc gacccctgcg ccgccatcca gcgctcgctg
     6601 accctggctc cgaacgaaga agctgaggtg gtgttccttc tcggccaggg caaagacgag
     6661 gccgaggccc ggacactggt ccaacgctat cgccggaccg accccgagga agtgaaggcc
     6721 gaagtggcga cccgctggaa tgacgtcctc ggcaacatcc gggtgaaaac gccggaccga
     6781 tctttcgact tgatgatcaa ccagtggctg ctctatcaga ccctcgcctg ccgggtgctg
     6841 gcccgaaccg cgttctatca gtctggtggg gcattcggtt tccgcgatca attgcaggac
     6901 gtcatggcgc tggtgacgcc gatgcgggat acggccagag agcatctgtt gcgtgctgcc
     6961 gcgcatcagt tcgaagaagg ggacgttctc cattggtggc acgagccctc cggtaagggg
     7021 gtgcgaaccc gcatctccga tgactacctg tggcttccct atgccgtcgc ccactatgtg
     7081 gaggtgaccg gcgaccaaga catattcgag gaggtagtgc cctatttgcg tgggatggag
     7141 ctcgccccgg atcaggacga gacattcttc acccccgagc gatcgtcgat agaaggaacg
     7201 gtgttcgacc actgccgacg tgctctcgac agggcgctgt ccaggctggg aagtcgcggc
     7261 cttcccctga tcggcaccgg tgattggaac gacggcttca accgggtggg tcgggagggg
     7321 cgaggtgaga gcgtgtggct cggatggttt ctcgaggcca acctgagccg attcgccgac
     7381 atcgccgacc ggtcgagtga gaccgagctc gccgccacct ggcggcgggg ggcgcttcgt
     7441 ctccgggcga gcctcgaggc cgaggcgtgg gacggcgact ggtaccggcg cgccttcttc
     7501 gacgatggga caccgctcgg ttcgtcggac aacccggagt gtcgaatcga ctcgatcgcg
     7561 caatcgtggt cggtcatcca cggcggggcg ccggcggaca gagcccagcg gtcgatggac
     7621 tcggtggagg aatacctggt ccatcgaggg gacggtctgg tgctgctctt caccccacca
     7681 ttcgacgatt gggaagtcga cccgggatac atcaaggggt acttgcccgg cgtgcgcgag
     7741 aacggcggcc agtacaccca cgccgcgatc tgggccgcga tggcgatgat cgaggccggg
     7801 cgcgtcgagg aaggctggcg
//
LOCUS       DASTXO010000447         4203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_579224, whole genome shotgun sequence.
ACCESSION   DASTXO010000447 DASTXO010000000
VERSION     DASTXO010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579224"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VFU96_09385"
     CDS             <1..619
                     /locus_tag="VFU96_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004671315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5113520.1"
                     /translation="AQKADSGEIFIDGEPVSIGTPRDAKAYGIETIYQTLALADNQDA
                     PSNMFLGRELRTRWGTLDDSAMEDATRKIMARLNPRFQKFKEPVSSLSGGQRQSVAIA
                     RAVHFNARILIMDEPTAALGPAETEQVRELMRQLKSEGIGIIMISHDIHDVFDLADRI
                     SVMLQGKLVGTVNKDDVTMDEVLAMIIIGKMPEEVTTKELEQLHG"
     gene            777..1898
                     /locus_tag="VFU96_09390"
     CDS             777..1898
                     /locus_tag="VFU96_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013094230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU5113521.1"
                     /translation="MPPDTVLLSPSRRNFLKALGMTAAIGLINACTNDGATATTAAGG
                     TGGTGSTAATGGSGAGNFPETPAWNFVFVNHVTTNPFFVPTVYGLEDAAAILGIPTPQ
                     WTGSEDADNGAMVNAMEAAIAAEADGIGVCLVDLEAFNGPTDEALAAGIPVIAYNADT
                     PPGNPNNRLCYVGQDLYGSGFEMGNRIVELVGSGKVALFIATPGQLNIQPRIDGALDA
                     IAESGADIEAIDIATGAALEEELTNVEAWDLGNQDATGMFAVDAGSTQAVGQVVRDQS
                     ARDNGLVGAGGYDLLPETVQLVSDGVLDFTIDQQPYLQGFLPTVYLYLYKLSGGVVTP
                     PETNTGLVFLDSASAQLFLETASRFEGDAEEQAIVEPVG"
     gene            1908..2918
                     /locus_tag="VFU96_09395"
     CDS             1908..2918
                     /locus_tag="VFU96_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5113522.1"
                     /translation="MTDTAVAPPRPQTIWGRVGGFLVARPESTIAIVALALGAYFAVV
                     NPAFLTTANIRVISQYMIATAILAAGQVMLLISGEIDLSMGNVYFMTPFLVFFASEAG
                     LVLPLAILAGVLGAGVVGYLNGAISLGFGIPSFIVTLGMGFVINGFTLNISDGFPKRV
                     PSTGVATEIFGGANFSGLMWVIIIVIAMHLILNHSRWGVYTVAVGGNPLGASEAGVPS
                     RNIKMRNFIVAAMLAGFAGVIEGIRVGSFDPGAGVAGTKVFESVAAAVIGGTALAGGS
                     GTIIGAFFGALVLGILRDGFNLEGVSAFTFTMVLGAAIILAMILNSLANKARIRSQSA
                     RL"
     gene            2915..3682
                     /locus_tag="VFU96_09400"
     CDS             2915..3682
                     /locus_tag="VFU96_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5113523.1"
                     /translation="MTSYANGEAIRAEGIGKTYGPVVALRDINLRLARGEVLGLIGDN
                     GAGKSTLIKILTGFTQPDQGQIFINGEETKLRSVSHARELGIETVYQDLALVNQLPVY
                     ANMSLNKEAVHRPIPFLDKKEMRDLARTHLGDMGVRIPSVDTEVGFLSGGQRQAVAIA
                     RAVYSDARVILLDEPLAAMGAKESAIIIDVIQGLRQKGDVSVILIAHNFAQVVEMCDR
                     VNLVQHGRITFDTQVSETSVQELTDLVSAEYRTRGIG"
     gene            complement(3685..>4203)
                     /locus_tag="VFU96_09405"
     CDS             complement(3685..>4203)
                     /locus_tag="VFU96_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEU5113524.1"
                     /translation="GAAEVPAGSDGLLFLPYLTGERTPHPDPLARGAFVGLTVRHDLR
                     HMTRAVMEGVAFGLRDGLDLMVAAGLVVPRQIRASGGGVRSELWRQILADVLDAEIAV
                     VGTEEGAAYGAALLAAVGVERFEAVEQAASEVIDVKPVAAPSSDRDAYGAAHTRFASL
                     YPALSPSFHALD"
BASE COUNT          769 a         1376 c         1343 g          715 t
ORIGIN      
        1 ggcccagaag gcggactctg gcgagatctt catcgacggc gagccggtca gcatcgggac
       61 cccgcgcgac gcgaaggcct acgggatcga gaccatctat cagaccctgg cgctggccga
      121 caaccaggac gccccgagca acatgttcct cggccgcgag ctgaggacgc ggtggggcac
      181 cctcgacgac tcggcgatgg aggatgccac caggaagatc atggcgaggc tcaacccgag
      241 gttccagaag ttcaaggaac cggtttcctc tctgtccggt ggccagcgcc aatcggtggc
      301 catcgcccgg gccgtccact tcaacgccag gatcctgatc atggacgagc cgaccgcggc
      361 cttgggcccg gccgagaccg agcaggtccg tgagctgatg cggcagctga agtcggaggg
      421 catcggcatc atcatgatca gccacgacat tcacgatgtc ttcgatctcg ccgaccggat
      481 ctccgtgatg ctccagggaa agctggtggg gacggtcaac aaggacgacg tcaccatgga
      541 cgaggtcctg gccatgatca tcatcggcaa gatgcccgaa gaggtcacca ccaaggagct
      601 ggaacagctc cacggctgag cccggctcga catcccactc tgcccaggtc gagatcgacg
      661 cgaccaccgc gacaaacttg tgaacgtata cccgcatacg cttgcaggcc tacggtccca
      721 tgagctatct tccaggattc cggggcaaca tgactgctcg acgaggagga aacgtcatgc
      781 caccagacac cgtattgctc agcccgtcgc ggaggaactt cctcaaggcg ctcgggatga
      841 ccgcggcgat cgggctcatc aatgcctgca ccaacgacgg ggccaccgcc accacggcgg
      901 ccggcgggac cggtggaacg gggagcaccg cggccaccgg cgggtcaggt gcggggaatt
      961 tcccggagac acccgcgtgg aacttcgtct tcgtcaacca cgtcaccacc aaccccttct
     1021 tcgttccgac tgtctacggg ctcgaggatg ccgcggccat cctcggcatc ccgacccctc
     1081 agtggaccgg gtcggaggac gccgacaacg gagcgatggt caacgccatg gaggccgcca
     1141 tcgccgccga ggcggatggg atcggtgttt gcctcgtcga tctcgaggcg ttcaatggcc
     1201 cgaccgacga ggcccttgcc gcgggaatac ccgtcatcgc ctacaacgct gacaccccac
     1261 ccggcaaccc gaacaaccgt ctctgctatg tggggcagga cctctacggc tccggcttcg
     1321 agatgggtaa ccggatcgtg gagctggttg ggtcaggcaa ggtggcgctg ttcatcgcca
     1381 ccccaggcca gctcaacatc cagcccagga tcgacggggc actcgatgcc atcgccgagt
     1441 caggcgccga catcgaggcg atcgacatcg ccaccggcgc cgcccttgag gaggagttga
     1501 ccaacgtcga ggcttgggac ctcggcaacc aggacgccac gggcatgttc gcggtggatg
     1561 ccgggagcac ccaggcagtt ggccaggtgg ttcgggatca gagtgcccgg gacaacggct
     1621 tggtcggggc gggggggtac gacctgctcc ccgagaccgt ccagctggtg tcggacggcg
     1681 tgctcgattt caccatcgac cagcagccct atctgcaggg gttcctgccc actgtctatc
     1741 tctatctgta caagctctcg ggtggtgtgg tcactccacc ggagaccaac accggtctgg
     1801 tgttcctcga ctcggcgtcc gcccagttgt tcctggagac ggcgagccgg ttcgaaggcg
     1861 atgcagagga acaggcgata gtcgagcccg taggctgatc ggaatagatg actgacactg
     1921 ctgtcgcccc gccccggccc cagaccattt ggggccgggt cggtggtttc ctggtcgcgc
     1981 gacccgagtc gaccatcgcc atcgtggcgt tggccctcgg ggcttacttc gccgtggtaa
     2041 acccagcgtt cctgaccacg gccaacatcc gggtcatctc ccagtacatg atcgcaaccg
     2101 cgatcctcgc cgccggccag gtcatgctcc tgatcagcgg ggagatcgac ctctcgatgg
     2161 ggaacgtcta tttcatgacc ccgttcttgg tcttcttcgc cagcgaggcc ggtctcgttc
     2221 tgcccctggc gatcctggcc ggggtgctcg gggccggggt ggtgggctat ctcaacgggg
     2281 cgatcagcct cgggtttggc atcccttcgt tcatcgtcac gctgggcatg ggtttcgtca
     2341 tcaacgggtt caccctcaac atctcggacg ggtttcccaa gcgcgtcccc tccaccgggg
     2401 tcgccaccga gatctttgga ggggccaact tctccgggct gatgtgggtg atcatcatcg
     2461 tcatcgcgat gcatctgatc ttgaaccact cccgatgggg cgtttacacg gtggcggtgg
     2521 gaggcaaccc gttgggagcg tccgaggcgg gtgtccccag ccggaacatc aagatgcgca
     2581 acttcatcgt cgccgccatg ctggccggct tcgccggggt gatcgagggg atcagggtgg
     2641 gctcgttcga tccgggagct ggcgtggccg gaacgaaggt gttcgagtcg gtggccgcgg
     2701 ccgtgatcgg cggcaccgca ctggcaggtg gctcggggac catcatcgga gccttcttcg
     2761 gcgctctcgt cttgggcatc ctgcgcgacg gcttcaacct cgaaggcgtc agcgccttca
     2821 cgttcacaat ggtcctcggg gcggcgatca tcctggccat gatcctcaac tccttggcga
     2881 acaaggcccg gatcagatcc cagagcgccc gattatgacc tcttacgcca atggtgaagc
     2941 gatcagggcc gaggggatcg gcaagaccta cgggcctgtc gtcgccttgc gtgacatcaa
     3001 tctccgtctc gccagaggag aggtgttggg gctgatcggc gacaacggcg ccgggaaatc
     3061 gactctcatc aagatcctca ccggcttcac ccagcccgat cagggccaga tcttcatcaa
     3121 cggcgaggag acgaaactgc gctccgtctc ccacgcccgc gagcttggga tcgagaccgt
     3181 ctaccaggac ctcgccttgg tcaaccagct accggtgtac gccaacatga gcctgaacaa
     3241 ggaagcggtg catcggccga tccccttcct cgacaagaaa gagatgaggg atctggcgcg
     3301 gactcacctc ggggacatgg gggttcgcat cccttctgtc gacaccgaag tcgggtttct
     3361 ctccgggggc cagcgtcagg cggtagccat cgcccgtgcc gtgtactccg acgccagggt
     3421 gatcctgctc gacgagccgc tggcggcgat gggagccaag gagagcgcca tcatcatcga
     3481 cgtgatccaa ggcttgagac agaagggcga tgtctccgtc atcttgatcg cgcacaattt
     3541 cgcccaggtc gtggagatgt gcgaccgggt caatctggtc cagcacggcc gcatcacctt
     3601 cgacacccag gtgagcgaga cctccgtcca ggagctgacc gacctcgtct cagccgagta
     3661 ccgcacccga gggatcggct gagctcagtc gagcgcgtga aaagaggggg agagggcggg
     3721 atagagcgag gcgaagcggg tatgggcggc gccgtaggca tcccggtcgg atgacggtgc
     3781 ggccaccggc ttcacgtcga ttacctcact tgcggcctgc tcgacagctt cgaatcgctc
     3841 gactccgacc gcagccagga gagcagctcc gtaggcagcg ccctcctcgg tgccgacgac
     3901 ggcgatctcg gcatcgagca cgtcggccag gatctgtcgc cacagctcac tccgcacccc
     3961 accccccgag gcccgtatct gccgaggaac gaccagtccc gccgccacca tgaggtcgag
     4021 gccgtcccgc agcccgaagg ccacgccctc catcaccgcc cgcgtcatat gccgcaggtc
     4081 gtgtcgcacg gtgagcccga cgaaggcacc ccgggccaac ggatcggggt gaggtgtgcg
     4141 ctcaccggtg agatagggga ggaagagcag tccatcgctc ccggcaggaa cctcggctgc
     4201 ccc
//
LOCUS       DASTXO010000448         2300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1020665, whole genome shotgun sequence.
ACCESSION   DASTXO010000448 DASTXO010000000
VERSION     DASTXO010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020665"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="VFU96_09410"
     CDS             <1..939
                     /locus_tag="VFU96_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HEU5113525.1"
                     /translation="RVRRPLTPLSPGTDLRVPGLSPFITPNDEFYRIDTALSVPRVNV
                     EEFSLRIFGMVDRELTLSYADLAARPQIESDITLTCVSNEVGGQLVGNARWQGIRLDA
                     LLEEAGVRPGADQVVGRSVDNYTCGFPVAAATDGRDAMIAIGMNGEPLPLVHGFPARL
                     VVPGLYGYVSATKWLKEIEITTFQSFDHYWARREWSAQAPIKTQSRIDTPGPLDNVPA
                     GPGTIAGVAWAQTRGIVKVEVKIDDGPWQVAQLADEVANTTWRQWRLRWDVTPGRHTI
                     TCRATDATGELQAEKRATPMPDGATGWHSIVVLGGD"
     gene            1011..2177
                     /locus_tag="VFU96_09415"
     CDS             1011..2177
                     /locus_tag="VFU96_09415"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEU5113526.1"
                     /translation="MQNTKSTRKPAAIVALLALVLAACGGGESSDATESAPPSTQAAT
                     PTTEATMEPTQPVTGLSAVNSGAACSAVPMDGEGSFDGMADDPAATAASNNPVLSTLV
                     TAVGEAGLVDTLNGEGPFTIFAPTDDAFAAIPEDQLNAILADQEQLTSILTYHVVAGE
                     SLDMQGLVDAGTVETVNGAELTIAPSGDLIEINGTAMVVCGNVQTANATVHIIDSVLV
                     PEAGEAMTDLSATNIGPACSAVPADGEGSFAGMADDPAATAASNNPVLSTLVTAVSEA
                     GLVDTLNGEGPFTIFAPTNDAFAAIPEDQLNAILGDPELLTSILTFHVVAGDDADLAQ
                     LIESGSVTTVQGEDLEIASNGDLASINGASVVCGNVQTANATVHIIDSVLVPAG"
BASE COUNT          424 a          792 c          728 g          356 t
ORIGIN      
        1 agagtgcgcc ggcccctgac cccgctcagc ccgggtaccg atctccgagt gccagggctc
       61 tctcccttca tcacccccaa tgacgagttc tatcggatcg atactgccct ctctgtcccc
      121 agggtgaacg tcgaggaatt ctcgctgcgc atcttcggga tggtcgaccg agagctgact
      181 ctcagctacg ccgacctggc ggcacgtccc cagatcgagt ccgacatcac tctcacgtgc
      241 gtctcgaacg aagtcggcgg ccagctggtg ggcaacgccc gctggcaggg aatccgcctc
      301 gacgcgttgc tcgaagaggc cggtgtgcga cccggagcgg accaggtcgt tgggcggtcg
      361 gtggacaact acacctgcgg attcccggtt gcggcggcga cggacggcag agacgccatg
      421 atcgcgatcg ggatgaacgg ggagccattg cccctcgttc acgggtttcc ggcccggctg
      481 gtcgtccccg ggctctatgg ctacgtctcg gcgaccaagt ggctgaagga gatcgagatc
      541 acgaccttcc agtccttcga ccactactgg gcccgtcggg aatggtcggc acaggcgccg
      601 atcaagaccc aatcccggat cgacaccccg ggcccactgg acaacgtgcc tgccggcccg
      661 ggcacgatcg cgggcgtggc ctgggcgcag acacggggaa tcgtcaaggt cgaggtcaag
      721 atcgatgacg gtccctggca ggtagcccag ctcgccgacg aggtggcgaa cacgacatgg
      781 cggcaatggc gtctgcgctg ggacgtgacc ccgggacggc acaccatcac ctgtcgagcg
      841 accgatgcga ccggcgagct ccaggccgag aagagggcaa ctccgatgcc cgacggcgcc
      901 accggctggc actcgattgt cgtcctcggc ggggactgag accaatccga ccccacgtcg
      961 gcggcgaaca caacgagacc cgaggctgat ccagccggaa aggaagcatc atgcagaaca
     1021 cgaagtcaac gaggaagcct gcggccatag tcgcactcct cgccctagtc cttgccgcgt
     1081 gcggaggcgg ggaatcatcg gatgcgacag aaagcgcccc acccagcacc caggcagcta
     1141 ccccgacgac cgaggcgacg atggagccga cccagcccgt gaccggcctg tcggcagtga
     1201 acagcggagc cgcatgctcg gcggtgccga tggacggtga gggctccttc gacggcatgg
     1261 ccgacgatcc ggccgccacc gcagcctcga acaaccccgt gctgtcgact ctcgtcactg
     1321 ccgtcggtga ggcaggcctg gtggacacct tgaatggtga ggggccgttc acgatcttcg
     1381 ccccaaccga cgacgccttc gcggcgatcc cggaggatca gctcaacgca atcctggccg
     1441 accaggagca gctcacctcg atcctcactt atcacgtcgt ggcgggggaa agtctcgaca
     1501 tgcagggtct cgtggacgcc gggacggtcg agacggtcaa tggcgccgag ctcacgattg
     1561 ccccttcggg tgatctgatc gagatcaacg gcactgcgat ggtcgtctgc gggaacgtgc
     1621 aaaccgccaa cgccacggtc cacatcatcg acagcgtgct cgtccctgaa gccggagagg
     1681 ccatgaccga tttgagcgcg accaatatcg gcccggcctg ctcggcagtg ccggcggacg
     1741 gcgaaggttc cttcgcgggc atggccgacg atccggccgc caccgcagcc tcgaacaatc
     1801 ccgttctctc cacgctggtg actgctgtga gcgaggcggg cctggtggac accctcaatg
     1861 gcgagggccc gttcacaata ttcgccccga ccaacgatgc cttcgccgcc atccccgagg
     1921 atcagctcaa cgccatcctc ggagacccgg agttgctgac gtcgatcctc accttccacg
     1981 tggtggccgg tgacgatgcc gacctcgctc aactgatcga gtccggctcg gtcaccaccg
     2041 tccagggcga agacctggag atcgcctcca acggcgacct ggccagcatc aatggcgcct
     2101 cagtggtctg cggcaatgtc cagaccgcca acgccaccgt ccacatcatc gactcggtcc
     2161 tcgtccccgc cggctagcag cggggggccg acgcggcgga tcccggcctc atcggccggg
     2221 atctgtcgcg cccacgacac gaagcaatgc ctgcctgcag tgctcctggt aatccagatg
     2281 gttggtccgc cagtcgctgg
//
LOCUS       DASTXO010000449         6316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_947938, whole genome shotgun sequence.
ACCESSION   DASTXO010000449 DASTXO010000000
VERSION     DASTXO010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6316
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947938"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="VFU96_09420"
     CDS             <1..1011
                     /locus_tag="VFU96_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24B family metallopeptidase"
                     /protein_id="HEU5113527.1"
                     /translation="PSAVRDITVVLSEMRLFKSHAEIEILRRAGELSARGHGEAMRYA
                     APGMHEYQVQAALEYYWRLGGSRHNGYPSIVASGPNTCILHYVENDRIIEDGDLLLID
                     AAAEVEGYSADITRTFPVNGIFSGPQRAIYEVVYAAFQAGLDMSAPGSSPRAIHDAAT
                     RVLTEGMVELGLLPRSVEESLAMHHYNQFYFHGTGHWLGLDVHDMGTYRVEGVPRKIE
                     PGMAFTVEPGLYIAPHKGEITLTMLEYDTDEWNQRRILEGRAAAMAKEAEAKEKAEKI
                     THSIPPELLGIGIRIEDDVVITEDGHDNLTSSVPSGIEEIEALCAESSALPSPQAVAG
                     RA"
     gene            complement(987..1649)
                     /locus_tag="VFU96_09425"
     CDS             complement(987..1649)
                     /locus_tag="VFU96_09425"
                     /inference="COORDINATES: protein motif:HMM:NF014997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEU5113528.1"
                     /translation="MERITLGRMQNPIRGFLHGAAAIATAVGLVLLMERASGRAEAVF
                     GALVFGGALFLMFIVSSLYHSVPWSDEWRARLQRIDHSMIFLVVAGTFTPIAISSLEG
                     VPLAVALGVVWGIALTGILLKALMPDIRTRLSVTLQMVMGWSALIWVPWIWRELGPGA
                     IGLIVLGGACYVVGTVIYATGRPRLFPRAFSHHELFHVLVIAGSSFHFFAILLYALPA
                     TA"
     gene            1756..2523
                     /gene="uppS"
                     /locus_tag="VFU96_09430"
     CDS             1756..2523
                     /gene="uppS"
                     /locus_tag="VFU96_09430"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEU5113529.1"
                     /translation="MALGVLYRLYERKLVNELDRDRLPRHIGIVLDGHRRHAREERLP
                     TYTESYRVGMRRFEEFIGWAEELEIPAVTAWVLSRENLSRPADELEPYFEVLVELFGR
                     LPALCQSYDMGIRFIGSLDLLPDELVAAAKMAEEAQPFGARRLNIAMGYGGRQEIVDA
                     AKDLVAKLVADGISPDEIGAHIDAGSLASHMYSADLPDPDLLIRTSGESRLSGFLLWQ
                     SAYAEFVFVDVNWPAFRRVDFLRALRDFTSRDRRFGK"
     gene            complement(2536..3222)
                     /locus_tag="VFU96_09435"
     CDS             complement(2536..3222)
                     /locus_tag="VFU96_09435"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU5113530.1"
                     /translation="MDLILVRHGQPDWTPGLIARNDPELTVLGHEQAKRVAHRLGAVA
                     AFDELWASPMLRAMETAAPVASEIDVEAEVYPWLREIQNPASWEGSPAEEIEKALRDA
                     NFRTMEEMWDGLPGGESFRAFHRRVVTGLEETLALHGIRPMHAEHRHLWEVEDPGMRV
                     VIVAHAGTNAVVLGRLLGLEPVPWEWDRFRQPHTGVSRLTMDRISTGWGFSLRQLGDV
                     SHLDEEMISA"
     gene            3282..3827
                     /gene="pyrE"
                     /locus_tag="VFU96_09440"
     CDS             3282..3827
                     /gene="pyrE"
                     /locus_tag="VFU96_09440"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU5113531.1"
                     /translation="MSRQALIDHLRHHALFTDGPYTLRSGQRSDWYLDARQTTFAGKG
                     APIVGAAVLDVLDPEVRAVGGMTMGADPIALAVAVVANLRGRDINAFSIRKTTKQYGI
                     TGRVVGPVTAGMPVAILEDTTTTGGAALDAAEAATAAGLRVVQAIALVDRSDGRAADA
                     FEDVSIPYVALVVPKDLGVKR"
     gene            3824..4411
                     /locus_tag="VFU96_09445"
     CDS             3824..4411
                     /locus_tag="VFU96_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113532.1"
                     /translation="MKVVVQRSAVRMWLLAMGGIPLLVISVDVLTSRRITNWLRELIF
                     TPQDTQIYEPRDVIWAWALLFFSVFLVVWGLKELFLPTKVIEARDEGLAVRLRGPFRH
                     PDLIPWGNIVDIEPGEIVDEGDVLLLLQIELVGRDGLPDHPWGARWVEARTLGLLAED
                     WSIAPARAAEQLGQYAVESAQREDRPRSARIPGPT"
     gene            4411..5172
                     /locus_tag="VFU96_09450"
     CDS             4411..5172
                     /locus_tag="VFU96_09450"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013827627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEU5113533.1"
                     /translation="MPVGAHLPSIDPLAEAQERQADIAQIFLSNPQSWKKPPPRQDAA
                     ALRESTIPIYVHAPYLINVCADNNRVRIPSRKILQDTCDAASEVGALGVIVHGGHVTG
                     DGPQEEGIDRWRKALDSVDTQVPILIENTAGGEKAVARRVEWIARLFEEIGRYEVGFV
                     LDTCHLWAGGEPLEDVVPRIMEATGRIDLVQVNDSKDPFDSRRDRHQSLGKGQIPAEE
                     MARMIIDAGAPVVVETPGGVEEQMADIEWIRALFR"
     gene            complement(5197..>6316)
                     /gene="secF"
                     /locus_tag="VFU96_09455"
     CDS             complement(5197..>6316)
                     /gene="secF"
                     /locus_tag="VFU96_09455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEU5113534.1"
                     /translation="FLAAGAVRGFGVTLTIGVLVSMFSALVVTRVLLEMLSRIDYLQS
                     RPRLMGLTVGRRLQEWLKTSGPNLFAKPQRWLIISGILIVLAVAGIVVNGLNFGIEFS
                     GGRLVEFSTTQEVDLDQLREDLADQGLPRALIQETGQGTIVIRTEELDAQGDQVLEDT
                     VAGVGGDVTLVRDQFVGPTLGEEIRNRALLALGLALLMQLIYLAIRFRWTIGAAAVLS
                     MFHDVIIVVGLFAWLGKTIDGVFLAALLTIIGYSIADSVVIFDRIREHQAERPGEPLG
                     PIANEACLQTVPRTINTGLGAILILASLYVLGGDTLSDFALALLIGILVGTYSSVFTA
                     TPLAILLEDRYPRPITEPAPSERKQHQKQKTGPKRIFE"
BASE COUNT         1181 a         2025 c         2047 g         1063 t
ORIGIN      
        1 ccgtcagcgg tgcgtgacat caccgtcgtc ctatcagaga tgcgtctttt caaatcgcac
       61 gcggagatcg agatcttgcg cagagccggt gagctgtctg ctcggggaca cggcgaggcg
      121 atgcgatacg ccgccccagg catgcacgaa taccaggtcc aggccgccct ggagtattac
      181 tggcggctgg gcggttcacg gcacaacgga tacccgtcca tcgttgcgtc cgggccgaac
      241 acctgcatcc tccattacgt tgagaacgac cggatcatcg aggacggcga tctcctcttg
      301 atcgatgctg ccgccgaggt ggaggggtat tcggcggaca tcacgcggac tttcccggtc
      361 aacggcattt tcagcgggcc tcagcgtgcc atctacgagg tcgtatacgc ggcgttccag
      421 gcgggtctgg acatgtctgc gcccgggtcg agcccgcgtg ccatccatga cgcggcgacc
      481 cgggtcctca ccgagggaat ggtggagctg ggccttctcc cacggagtgt cgaagagtcc
      541 ctggcgatgc accactacaa ccagttctac ttccacggca ccggtcactg gctcggtctg
      601 gatgtgcacg acatgggcac ctatcgcgta gagggggtgc cgaggaaaat cgagcccggc
      661 atggccttca cggtcgagcc tggcctctac atcgccccgc acaagggcga gatcaccttg
      721 accatgctcg agtacgacac cgacgagtgg aaccagcgtc ggatcctcga aggacgtgcc
      781 gccgccatgg ccaaggaagc agaggcgaag gaaaaggcgg agaagatcac ccattcgatt
      841 ccgccggagt tactcggcat cgggatccga atcgaggatg acgtggtgat caccgaagac
      901 ggtcacgaca acctgacgag ctcggtccct tcaggcatcg aagagatcga agccctgtgc
      961 gccgagtcct ccgctcttcc cagccctcag gcagtcgccg gaagggcgta gagcaggatg
     1021 gcgaagaaat gaaagctgct cccggcgatc accaggacgt ggaacagctc gtggtgtgag
     1081 aaggctcgcg ggaataggcg ggggcgcccg gtggcataga tcaccgtgcc gaccacgtag
     1141 caagccccgc cgaggacgat gagaccgatg gcgcccgggc cgagctcacg ccagatccag
     1201 gggacccaga taagcgccga ccaccccatc accatctgaa gcgtcaccga gagcctcgtg
     1261 cggatgtcag gcatgagtgc cttgagcagt atcccggtga gcgcgatgcc ccagacgacg
     1321 ccgagtgcaa cggcgagcgg gaccccttcg agagaggaaa tggcgatcgg ggtgaaggtg
     1381 ccggctacca cgaggaagat catcgaatgg tcgattcgct gcagcctcgc cctccactcg
     1441 tccgaccacg ggaccgagtg ataaagcgac gagacgatga acatcaggaa caacgctcca
     1501 ccgaagacga gagccccgaa gacggcttca gcccgtcccg acgccctttc cattaggagc
     1561 acgagcccga cggcggtggc gatcgctgcc gcgccgtgca gaaagccccg aattgggttc
     1621 tgcattctcc ccagggtgat ccgttccatg tcgcagggaa gggtatcggt cgcaccggtg
     1681 taggtggaaa gggtcggagg acccaggacg aatcgagacc tggttcggac agaccggtac
     1741 catgacggca tcaccatggc tcttggtgtc ctctaccgcc tatacgagcg caagctcgtc
     1801 aacgagctcg atcgagaccg gctgccccgt cacatcggca tcgtcctcga tgggcatcgt
     1861 cgccatgccc gtgaggagcg gctgcccacc tacaccgaga gctaccgagt cggcatgcgc
     1921 cgcttcgagg agttcatcgg atgggccgag gagctcgaaa tcccggccgt caccgcctgg
     1981 gtcctgtcgc gggagaatct gtcgcgaccg gccgacgagc tcgagcccta cttcgaggtg
     2041 ctggtggagt tgttcggccg gctgccggcc ctgtgtcaga gctacgacat gggcatcagg
     2101 ttcatcggga gcctcgactt gcttcctgac gagctggtgg ctgcagccaa gatggcggag
     2161 gaggcccagc cttttggcgc ccgacgtctc aacatcgcga tgggctatgg gggaaggcag
     2221 gagatcgtcg acgctgccaa ggacctcgtg gccaagctcg tggccgacgg catctctccc
     2281 gacgagatcg gcgcgcacat cgacgccggg tcgctcgcct cccacatgta ctccgcagat
     2341 ctgcccgacc cggatcttct gatcaggact tccggcgagt cgcggctctc cggattcctg
     2401 ctctggcagt cggcctacgc cgaattcgtc ttcgtggacg tgaattggcc cgcgttccgt
     2461 cgggtcgact tcctccgcgc tctccgtgat ttcaccagcc gagaccgccg tttcgggaag
     2521 taggcggccg gcgaatcaag cagagatcat ctcctcgtcg agatggctca cgtctccgag
     2581 ctggcgtaac gagaaacccc acccggtgct aatccggtcc atcgtcaatc gggagacccc
     2641 ggtgtggggc tgacgaaacc ggtcccattc ccaggggacc ggctccaacc cgagcagacg
     2701 acccaggacc acggcgttcg tgcccgcatg agccacgatg acgacccgca tcccggggtc
     2761 ctcgacctcc cagaggtggc gatgctcggc gtgcatcggc cggatgccat gaagggccag
     2821 ggtctcctcg agcccggtca ccacccggcg gtggaaggcg cggaaagact ccccgccagg
     2881 gagcccgtcc cacatctcct ccatcgtccg gaagttggcg tccctcagcg ccttttcaat
     2941 ctcctcggcc ggcgatccct cccaggacgc tggattctgg atctccctca gccagggata
     3001 cacctctgcc tcaacgtcga tctcggacgc caccggggct gccgtctcca tcgcccggag
     3061 catcggcgat gcccagagct cgtcgaaggc tgcgaccgca cccagccgat gagccactcg
     3121 cttcgcctgc tcatggccca ggaccgtcag ctccgggtcg ttgcgggcga tcaggcctgg
     3181 ggtccagtcg ggttggccat gtcggacgag gatcaagtcc atcggcccca ccgtacccag
     3241 cccgttgtcc ataccggccg gcgcggcaat acgattgggg ggtgtcccgg caagccctca
     3301 tcgatcacct gcgtcaccac gccctcttca ccgatggccc ctacaccctg aggtcaggcc
     3361 aacggtcaga ttggtacctc gacgcacggc agacgacgtt tgccggaaaa ggggcgccca
     3421 ttgtcggcgc ggccgtgctc gatgtcctcg accccgaagt ccgggccgtc ggaggcatga
     3481 ccatgggggc agatccgatc gccctcgccg tcgcggtcgt tgccaatctc cgaggccgcg
     3541 acatcaacgc tttctcgatt cggaagacaa cgaagcaata cgggatcacg ggtcgggtgg
     3601 ttggcccggt gaccgccggg atgccggtgg cgatcctcga agacacgacc accacaggtg
     3661 gcgccgccct ggacgcggca gaggccgcca ccgcggccgg actgcgggtg gtgcaggcga
     3721 tagctctcgt cgatcggtcc gatgggaggg cagccgacgc cttcgaagac gtgtccatcc
     3781 catatgtggc cctcgtggtg cccaaggacc tgggcgtcaa gaggtgaagg tcgtcgtcca
     3841 gcgctcggcg gtcagaatgt ggctgctggc gatgggcggc ataccactgc tcgtcatctc
     3901 cgtggacgtg ctcacgagcc ggcggatcac caactggctg agggagctga ttttcacgcc
     3961 gcaggacacg cagatctacg agccacggga tgtgatttgg gcctgggccc ttcttttctt
     4021 ctctgttttc ctggtcgtct ggggtctcaa ggagctcttt ctgccgacca aggtgatcga
     4081 ggctcgggat gagggcctcg cggtcaggct gcggggcccg tttcgccacc cggacctcat
     4141 tccctggggc aacatcgtcg acatcgagcc gggcgagatc gtggacgagg gagacgtgtt
     4201 gcttctgctt caaatcgagc tcgtcggccg tgacggcctc cccgaccatc cctggggggc
     4261 gcgctgggtg gaggcacgca ccctgggcct cctcgccgaa gactggtcga ttgctccggc
     4321 ccgggcagcg gaacagttgg gccagtacgc ggtcgaatcg gctcaaaggg aggatcgacc
     4381 ccggagtgct cgcatcccgg gcccgacgtg atcccggtcg gcgcccacct gccgtcgatc
     4441 gatccgttgg ccgaggcgca ggagcgccag gccgatattg cccagatctt tctatccaac
     4501 ccccagtcgt ggaagaagcc gcccccccga caggatgcag ccgccctgcg tgaatcaacc
     4561 atcccgatct atgtccacgc tccatatctg atcaatgtct gtgcagacaa caaccgggtg
     4621 aggatcccat cccggaagat cctccaggac acctgcgatg ccgcgtctga ggtgggtgcc
     4681 ttgggtgtga tcgtgcatgg tggccacgtg accggcgacg gtccgcagga ggagggcatc
     4741 gatcgatgga ggaaggccct cgattcagtg gacacccagg tcccgatcct gatcgagaac
     4801 accgcgggcg gggagaaggc ggttgcgaga agggtggagt ggatagcacg cctcttcgag
     4861 gagatcggcc gctacgaggt tggcttcgtc ctcgacacct gtcatctgtg ggcgggtggg
     4921 gagccgctgg aggacgtcgt gccccgcatc atggaggcga cgggtcggat cgatctcgtc
     4981 caggtcaacg actcgaagga cccgttcgac tcacgacggg atcgtcacca gagcttggga
     5041 aaagggcaga tccctgccga ggagatggcc cggatgatca tcgacgccgg tgccccggtc
     5101 gtggtggaga caccgggtgg tgtcgaggag cagatggccg acatcgagtg gattcgcgcc
     5161 ctcttcaggt gaagaggcag cagacacaaa agccccctac tcgaagatcc gcttcggccc
     5221 tgtcttctgc ttctgatgct gcttgcgctc actcggtgcc ggctcggtga tcgggcgcgg
     5281 gtaacgatcc tcgaggagga tggcaagcgg ggtggcggtg aacaccgagg agtaagtccc
     5341 gaccaggatc cctatcagca aggcgagggc gaagtccgag agcgtgtcac cgccgagcac
     5401 gtacagcgaa gcgaggatga ggatcgcccc cagtccggtg ttgatcgttc tcggcactgt
     5461 ttgcaggcag gcctcgttgg cgatggggcc taagggctcg ccgggccgtt cggcctggtg
     5521 ctcgcggata cggtcgaaga tcaccaccga atcggcgatc gaatagccga tgatcgtcag
     5581 caacgcggcg aggaagacac cgtcgatggt cttgcccagc caggcgaaca gcccgacaac
     5641 gatgatgaca tcgtgaaaca tcgacagaac ggccgccgct ccgatggtcc agcggaacct
     5701 gatcgccagg tagatgagtt gcatgagcag tgccaggccg agggccagca gggcgcggtt
     5761 ccggatctct tcgcccagcg tgggcccgac gaactggtcc cggaccagag tcacgtcacc
     5821 gccgaccccg gccaccgtgt cctcgagcac ctggtccccc tgggcgtcga gctcctcggt
     5881 acggatcacg atggtgccct gcccggtctc ctggatcaga gctcgcggca gcccctggtc
     5941 cgccaaatcc tctcgcagct ggtcgaggtc tacttcttgg gtagtcgaaa actcgacaag
     6001 ccttccgcct gagaactcga tcccgaagtt gaggccattc accacgatgc cggccacggc
     6061 gaggacgatc agaatcccgg agatgatcaa ccagcgctgc ggcttggcga acagattcgg
     6121 ccccgaggtc ttcagccatt cctggagtcg ccgcccgacg gtcaggccca tcaaccgggg
     6181 ccgggattgc aggtagtcga tccgtgacag catctcgagc agcacccggg tgacaacgag
     6241 cgcggagaac atcgagacca gcaccccgat ggtgagcgtg accccgaaac cgcggaccgc
     6301 gccggcggcg aggaag
//
LOCUS       DASTXO010000450         6806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1168467, whole genome shotgun sequence.
ACCESSION   DASTXO010000450 DASTXO010000000
VERSION     DASTXO010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6806
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1168467"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFU96_09460"
     CDS             <1..258
                     /locus_tag="VFU96_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU5113535.1"
                     /translation="IETPLRPPQRLHEAALISFEIARRHTYHAAVASLPEGIGLHLLP
                     SGNPVAFDDARQLKWRDVSATTGLIDGAYTASKAYLEGERS"
     gene            297..1289
                     /locus_tag="VFU96_09465"
     CDS             297..1289
                     /locus_tag="VFU96_09465"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU5113536.1"
                     /translation="MIDPAIVLGVGLAVISLPVWIIGAAFVSRYVPGNWRILRIAWFL
                     FLYLVVEAVSLCVMFLTWVFSGFGWKMRSPTFTEFHYRLLAVMLRTVIRSAEVTFRLR
                     VVQDEQQRPTTAVNGTTRSILVLSRHAGPGDSFLLMDGLVNGYGRDPRIVLKEFLQWD
                     PAVDVILNRLPAAFVPVGRKGGQELIDAISGLARSMDDDDAFVIFPEGANYTELRRKR
                     AIQKLREIGRPDLAERAEELKQTLPPRSLGVITALAAAPADSDVFFVGHAGLEAFITA
                     GDIWRAMPMDTEVEVKIWHFRAEDIPPPEEQETWLYDVWAEIDDWISAELARTG"
     gene            1299..1817
                     /locus_tag="VFU96_09470"
     CDS             1299..1817
                     /locus_tag="VFU96_09470"
                     /inference="COORDINATES: protein motif:HMM:NF008740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEU5113537.1"
                     /translation="MLTIAGNILWLILCGIWMAILYFIGGLLGLILIITIPLGIQSFK
                     LGNYVLWPFGRTVVTEPGASVVWSVIGNVIWVIVAGWWLALFHVALAVIFFITIIGIP
                     FGIANLKLARLALLPFGAIVVTEVPPDAQTIVVVPQLGATAAPQSASPAASSPQMGGT
                     EPPEITGRSSAP"
     gene            complement(1792..2796)
                     /gene="ilvC"
                     /locus_tag="VFU96_09475"
     CDS             complement(1792..2796)
                     /gene="ilvC"
                     /locus_tag="VFU96_09475"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016205830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU5113538.1"
                     /translation="MATIYYDADSDPDLIRAKKVAVIGYGSQGHAHALNLKESGVDVV
                     VGLREGSSSWAEAEGEGLRVMTPEAAAEWADTIMMLVPDQVMGTIYAESVERNLDSGD
                     TLLFAHGFNIHFGEIVPPEDVDVAMIAPKGPGHLVRRTYTEGSGVPCLLAVHQDASGN
                     ARALGLSYASAIGGGRAGILETTFKEETETDLFGEQVILCGGVSELIKASFETLVEAG
                     YQPESAYFETLHELKLIVDLLYEGGLSWMRYSVSDTAEYGDYTRGSRIITDETRAAMK
                     EALADIQSGAFANEWMAQAREGAPFLYEQRAANRAHQIEDVGRELRKMMPFMEPKTAP
                     "
     gene            2857..4524
                     /gene="ilvD"
                     /locus_tag="VFU96_09480"
     CDS             2857..4524
                     /gene="ilvD"
                     /locus_tag="VFU96_09480"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU5113539.1"
                     /translation="MTQKIRSQEVTEGPAKAGARAMLRAVGLTEEDFDRPQVGVVSAG
                     NEVTPCNLTGPELSRHAKTGVAAAGGVGLVFSTIAVSDGISMGHEGMRASLVSREVIA
                     DSVELVMHAERFDALVAIAGCDKSLPGMLMAAARLDLPAVFLYGGSSLPGHYAGRDIS
                     IVDVFEGIGAESEGRITIEELLEIERVACPGAGSCAGMFTANTMASVGEAIGMSLPGS
                     ASIPAVDPRLHEFAERSGEAVMKLLDDRVRPRDIMTPHAFENAITTVMALGGSTNAVL
                     HLLAIAHEAGVELELEDFDRISRHTPHLGDLKPFGKYHMVDLDRVGGVPIVLRALLDE
                     GLLHGDTLTVTGRTMAENLSDSVFNREQDVIAPPSQPLAAEGGIAVLRGNLAPGGAVV
                     KVAGIDLDVFEGPARVFDGEQGAIQALFDHQIKSGDVVVIRTEGPKGGPGMREMLQIT
                     AAIKGAGLGKEVLLVTDGRFSGGTTGLCIGHVAPESEAGGPMAWVEEGDRVRVDVPAR
                     RLDVLVSDDVLADRAAHWSPHPARYETGALAKYARLVGSAETGAVTG"
     gene            4535..5512
                     /locus_tag="VFU96_09485"
     CDS             4535..5512
                     /locus_tag="VFU96_09485"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEU5113540.1"
                     /translation="MSAEPIPQDREKRGFFKRAARGASERVLDVVDPDMVLEHVDVNA
                     LLERIDINSLLDRIDVERLIGRIDIDELLKAADIDALMERVDIKALIDRAGIPDIVAE
                     STSHLTGSALDMFRRPIVGIDEILFKGINRLFRRDLRTFPTGPGALVDWVDDNTEDIG
                     VRTGRYAGPLTRTIALLLDILIVTTGFTVIVAGASYLIELVSPVEVEPTRGLWYIVGF
                     VVWALLYLWLSIAIFGKTLGKTFMGVRVVDSEGSVALHGRQSLIRAVTYPLSFVVLGL
                     GLLGILFGRERRAWHDHFAGTAVVYDWGSRTARLPTPLAAWLERKGMES"
     gene            complement(5514..6620)
                     /locus_tag="VFU96_09490"
     CDS             complement(5514..6620)
                     /locus_tag="VFU96_09490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5113541.1"
                     /translation="MTDFAVIGGGIAGVSAAAHLAPYGSVTLFESERTLAFHTTGRSA
                     AMFVANYGAEGSRPLGLASRAFFEHPPDGSVDTPLLSDRGLLWISDQTRLDRLRTIAS
                     EGDALGAGSVLVDSHEILRLVPKLRPERIAGGLYEPNALDIDVAALHQAFVRIARKAG
                     AEIRVSSPVTGLARLGSGWRVSTPDDNIDCTSVVNASGAWGDIVARLAGIEPVGLQPM
                     RRTAFMVPGDQAYARWPFVVDVEQLFYFRPDGAQLLCSLAEETPSPPLDPRPDMADVA
                     LAIERINEATTLDIRSVNSQWTGLRTFAPDREMVIGEEPTAPGFFWLVGQGGTGIQTA
                     PAYGALVASQVLGSGMRPDLSDIDPAVTSPIRFR"
     gene            complement(6657..6785)
                     /locus_tag="VFU96_09495"
     CDS             complement(6657..6785)
                     /locus_tag="VFU96_09495"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEU5113542.1"
                     /translation="MDWVADGTLRPLVQKTLPLDGAVEALRWVAERKVVGRVVITP"
BASE COUNT         1166 a         2128 c         2246 g         1266 t
ORIGIN      
        1 atcgagacgc cccttcgacc accgcagcgg ctccacgagg ccgctctgat ctccttcgag
       61 atcgcacgtc gtcataccta ccacgcagcg gttgccagcc tgcccgaggg aatcggtctt
      121 cacctcctcc cctcgggaaa ccccgtcgcc ttcgatgacg ccagacaact gaaatggagg
      181 gacgtgagcg cgacgaccgg gctcatcgac ggcgcataca cagcgtcgaa ggcctatctg
      241 gaaggggaac gctcgtgaag gtgccgccgt tgccgccacg ctggttacga cggctggtga
      301 tagaccccgc gatcgtcctc ggcgtgggtc tggcggtgat ctcgctcccg gtttggatca
      361 tcggtgctgc cttcgtctcc cggtatgtgc ccgggaactg gcgcatcctg cgcatcgcct
      421 ggttcctttt cctgtatctg gtggtggagg cggtgagcct ttgcgtcatg tttctcacct
      481 gggtgttctc cggcttcggc tggaagatga gatcgcccac cttcaccgag ttccattacc
      541 gccttctcgc agtgatgctg cggacggtga tcaggtcggc ggaggtgacc ttcagactcc
      601 gagtggtgca ggacgagcag cagcggccga ccaccgctgt gaacggcacc accagatcga
      661 tcctggtgtt gtccagacat gccggtcccg gcgactcttt cctcctcatg gacgggctgg
      721 tcaacggata cgggcgcgac ccgcggatcg tcctcaaaga gttccttcag tgggacccgg
      781 ctgtcgatgt gatcctcaac aggctgcctg cggcgttcgt cccagtcggg cgcaaggggg
      841 gacaggagtt gatcgacgcc atctccggcc tggctcgctc gatggacgat gacgacgcct
      901 tcgtcatctt tccggaaggt gccaattaca ccgagcttcg acggaagcga gcgatccaga
      961 aactgaggga gatcggtcgc cccgacctcg ccgaacgcgc cgaggagctg aaacagactc
     1021 tcccgcctcg ttccctcgga gtgatcacgg cattggcggc ggcgcccgct gattcggatg
     1081 tgttcttcgt ggggcacgcc ggtctggagg ctttcatcac cgccggtgac atctggagag
     1141 cgatgccgat ggacaccgag gtcgaggtga agatctggca tttccgggcc gaggacattc
     1201 ccccacctga ggaacaggag acctggctgt atgacgtctg ggccgagatc gacgactgga
     1261 tcagcgccga attggcgagg acgggttagg ttcaagccat gttgacgatc gctggcaaca
     1321 tcctctggtt gatcctgtgc gggatctgga tggcgatcct ctacttcatc ggcggcctgc
     1381 tcgggctgat cctgatcatc accatcccgc tcggcatcca gtccttcaag ctggggaact
     1441 acgtcttgtg gccattcggg aggaccgtgg tgaccgagcc gggggcatcg gtggtctggt
     1501 cggtgatcgg gaatgtgatt tgggtgatcg tcgccggttg gtggctggcc ctatttcacg
     1561 tggcgctggc ggtcatcttc ttcatcacga tcatcggaat ccctttcggc atcgccaatc
     1621 tgaagctggc acggctggcc ctgcttccgt tcggggcgat agtggtgacc gaggtgccac
     1681 ccgacgcgca gacaatcgtc gtcgtgcccc aattgggagc aaccgcggca ccgcagtcgg
     1741 cgtcgccggc ggcatcgtct ccacagatgg gcgggactga gccgcccgag atcacggggc
     1801 ggtcttcggc tccatgaagg gcatcatctt gcgcagttcc cggcccacgt cttcgatctg
     1861 atgggcccgg ttcgccgccc gttgctcgta gaggaacggc gctccctctc gagcctgagc
     1921 catccattcg ttggcgaagg cgcctgactg gatatcggcc agggcttctt tcatcgccgc
     1981 ccgcgtctcg tcggtgatga tccgagagcc acgtgtgtaa tccccgtact ctgcggtgtc
     2041 tgataccgag taccgcatcc aggagaggcc tccctcgtag aggagatcga cgatgagctt
     2101 gagctcgtgg agcgtctcga agtaggccga ctccggctga tagccggcct cgaccagtgt
     2161 ctcgaaagag gccttgatca actcggacac cccaccacac aagatgacct gctcgccgaa
     2221 gagatcggtc tcggtctcct ctttgaacgt ggtctccagg atgcccgccc tgcccccacc
     2281 gatcgccgat gcgtaggaca gccccagggc gcgggcgttt cccgatgcgt cctggtgcac
     2341 tgcgagcaga cacggaaccc ccgatccctc ggtgtaggtg cgccggacca gatggcccgg
     2401 gcccttgggg gcgatcatcg ccacatcgac gtcctccgga ggcacgatct caccgaaatg
     2461 gatgttgaag ccgtgggcga agagcagggt gtcgcccgaa tcgaggttgc gctccaccga
     2521 ctcggcatag atggtcccca tcacctggtc gggcacgagc atcatgatcg tgtccgccca
     2581 ctcggccgca gcctccggtg tcatcacccg aagaccttcc ccttcggcct cggcccaact
     2641 cgacgagccc tctcgaagtc ccacgacgac gtcgaccccg gattccttca ggttgagggc
     2701 atgggcgtgt ccctggcttc cgtagccgat gacggccacc ttcttggctc ggatgaggtc
     2761 gggatcgctg tcggcgtcgt agtagatggt tgccatgccc ggattgtggt cgttttcgcc
     2821 ttggggaaaa actcggcgtc ataggatgcc tggcccatga cacagaagat cagatctcaa
     2881 gaggtgacgg aaggaccggc caaggctggg gcgagggcga tgctgcgcgc cgtcggcctc
     2941 accgaggagg acttcgatag gccgcaggtc ggggtggtct ccgcaggcaa cgaggtcact
     3001 ccctgcaacc tcaccgggcc cgagctgtcg aggcacgcca agacgggcgt ggcggcggcg
     3061 gggggagtgg gactggtctt ctcgacgatc gccgtctcgg atgggatctc gatgggtcac
     3121 gagggaatgc gggcgtcgtt ggtctctcgg gaggtcatcg ccgattcggt cgagctggtg
     3181 atgcatgcgg agagattcga tgccctggtc gccatcgcag ggtgcgacaa gtcgttaccc
     3241 gggatgctga tggccgcggc gcggttggac ctgccggccg tctttctcta cggaggctcc
     3301 agcctccctg gccactacgc cgggcgtgat atctccatcg tcgacgtgtt cgaggggatc
     3361 ggcgccgaat cagagggccg gatcaccatc gaggagctgc tcgagatcga gcgagttgcc
     3421 tgcccaggtg cagggtcctg tgccgggatg ttcaccgcca acacgatggc gtcggtaggg
     3481 gaggcgatcg ggatgtcgct gcccgggtcc gcctcgatcc ccgccgtcga cccgcgcctc
     3541 cacgaatttg ccgagcggtc gggagaggcc gtgatgaagc tcctcgacga ccgtgtccgc
     3601 cctcgggaca tcatgactcc acacgcattc gagaacgcca tcaccacggt gatggccctg
     3661 ggtggctcga cgaatgccgt gctgcacctc ttggccatcg ctcatgaggc gggtgtggag
     3721 ctcgagttgg aggacttcga ccgtatctcc cgacacacgc ctcatctcgg tgatctcaag
     3781 ccattcggca agtaccacat ggtcgacctc gaccgggtcg ggggagtgcc gatcgtgttg
     3841 cgcgccctgc tcgacgaggg attgctgcac ggggacaccc tgacggtgac cggtcgcacc
     3901 atggcggaga acctgtcaga cagcgtcttc aatcgtgagc aagacgtgat cgcgccaccc
     3961 tcgcagccct tggccgccga gggggggatc gccgttctca ggggcaacct ggctcccggt
     4021 ggcgccgtgg tcaaggtggc cgggatcgac ctcgatgtgt tcgagggccc ggcgagggtc
     4081 ttcgatggcg aacaaggggc gatccaggcg ctgttcgatc accagatcaa gtcgggtgat
     4141 gtagtcgtga tcaggaccga ggggcccaag ggaggtcccg ggatgcggga gatgctccag
     4201 ataaccgctg ctatcaaagg agcgggtctg ggcaaagagg tgctcctcgt gaccgatggg
     4261 aggttctccg ggggcaccac gggcctttgc atcggtcatg tggcccctga gtccgaagca
     4321 ggcggcccta tggcctgggt ggaggagggc gaccgggtcc gcgtcgatgt cccggctcgc
     4381 cgtctcgatg tcctggtttc ggacgacgtg ctggctgacc gcgccgccca ttggtcaccc
     4441 catccggcga ggtacgagac cggcgccctg gccaagtatg cgcgtctcgt cggttcggcc
     4501 gaaaccggag cggtcaccgg ctagcgtctc cagggtgagc gcggaaccga tcccacaaga
     4561 cagggaaaaa cgaggcttct tcaagcgcgc agcccgcggg gccagtgaac gggtgctgga
     4621 cgtcgtcgac ccggacatgg tgctcgaaca tgtcgatgtg aatgcccttc tcgaacgcat
     4681 tgacatcaat tcgcttctcg atcgcatcga tgtcgagcgc ctgatcgggc gaatcgacat
     4741 cgacgagctg ctcaaggcgg ccgatatcga cgcgctcatg gagcgggtcg acatcaaggc
     4801 cctgatcgat cgggccggga tccccgacat cgtggctgag agcaccagtc acctcaccgg
     4861 atcggcgctc gacatgttcc ggaggcccat cgtggggatc gacgagatcc tgttcaaggg
     4921 catcaaccgc ctgttcagac gggaccttcg gacctttccc accgggcccg gggctctggt
     4981 cgactgggtc gacgacaaca ccgaggacat cggggtccgg acggggcgat acgccggccc
     5041 actgacccgg acgatcgccc tgctgctgga catactgatc gtcaccaccg ggttcaccgt
     5101 gatcgtggct ggcgcgagct acctgatcga gctggtcagc ccggtcgagg tcgagccgac
     5161 gcgagggttg tggtacatcg tcgggtttgt cgtgtgggcg cttctctatc tgtggctctc
     5221 catcgcgata ttcggcaaga cgttgggcaa gaccttcatg ggcgtccggg tggtggattc
     5281 cgaggggtcg gtggccctgc acggccgcca gtccctgatt cgcgcagtca cctacccgct
     5341 ttcctttgtc gttctgggtc tgggcctgct cgggatcttg ttcggccggg agcggcgggc
     5401 ctggcacgac catttcgccg ggaccgccgt ggtctatgac tggggaagcc gcactgcacg
     5461 cctcccgaca ccgctcgctg cgtggctgga acgcaaaggc atggagagct gacctaacga
     5521 aagcggatcg gcgaggtgac ggccgggtct atatccgaca gatcaggacg cataccggag
     5581 ccgagcacct ggctggcaac gagcgcccca taggcggggg ccgtctggat gccggtgcca
     5641 ccctggccca ccagccagaa aaagcccggg gcggtcggct cctccccgat gaccatctcc
     5701 cggtccggcg cgaatgtccg caatccggtc cattgcgagt tcaccgacct gatgtcgagg
     5761 gtggttgcct cgttgatcct ctcgatggca agggcgacgt cggccatgtc gggccgcggg
     5821 tcgagcgggg gagagggagt ctcctcggcc agcgagcaga gcagctgggc tccgtcggga
     5881 cggaaataga agagctgttc cacatccact acgaacggcc acctggcgta ggcctgatca
     5941 ccaggaacca tgaacgcagt tcggcgcatc ggctgcagcc ctactggttc gataccagcc
     6001 agcctcgcaa cgatgtcacc ccaggcgcct gaggcgttga cgacagatgt gcagtcgatg
     6061 ttgtcgtccg gagtagacac ccgccatccg ctgccaagcc tggccagacc ggtgaccggc
     6121 gacgaaaccc gtatctcagc ccctgccttt ctggcgatgc gaacgaaggc ctgatggagg
     6181 gcggcgacat cgatgtcgag ggcgttcggc tcatagagac ccccggcgat gcgctctggc
     6241 cgtagctttg gcacgagccg cagaatctcg tggctgtcga ccaacaccga tccggcgccg
     6301 agtgcatcgc cctcggatgc gatggttcgc aaacggtcga gtcgggtctg atcggagatc
     6361 cagaggagac cgcggtccga gaggagcggg gtgtctaccg agccatcggg cgggtgttcg
     6421 aagaacgccc tgctcgccaa cccaagcggg cgtgagccct cggctccgta gttggcgacg
     6481 aacatggctg cggaccgccc agtggtgtgg aaggcgaggg ttcgctcgga ttcgaacagc
     6541 gtcaccgagc cgtagggggc gagatgagca gctgccgaca cgccagcgat cccaccgccg
     6601 atcactgcga aatcggtcat gagaggtccg tcacccgaat ggtcaaacgg ggccggtcat
     6661 ggagtaatca ccaccctccc gaccaccttg cgttcggcga cccaacggag ggcctcaacc
     6721 gcgccgtcca gggggagtgt cttctgtacc aaagggcgca gggtcccatc cgcgacccag
     6781 tccatcacga tctggttgtt ctcggc
//
LOCUS       DASTXO010000451         2252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_247399, whole genome shotgun sequence.
ACCESSION   DASTXO010000451 DASTXO010000000
VERSION     DASTXO010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247399"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1978
                     /locus_tag="VFU96_09500"
     CDS             <1..1978
                     /locus_tag="VFU96_09500"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEU5113543.1"
                     /translation="NRSQIPMKFALRLTTLGVIFTVMFSVIGLRLWFIQVAEGPQIAR
                     AAEEATWVGKTTYAARGDIYDRNGTLLATSRMVPAVMVDRTFVQPDQREPLIQELASI
                     VGVDPVQLDDMYEEAGINGRFQVSTVSNETAYAINESLDELPGVEVVKVPERVYLAGP
                     TMAHVIGHLGLPEEADLAEREDLEPDVRIGKLGVERVYDEVLQGTGGSVEYRVRRGEI
                     IEQRPPVDPVTGSSLVLNLDLALQEQVELALESGVALSNEVKEEERADGEEVFNETQR
                     AAAVVLDVDTFDVLALASFPDFDPQLFVAGIDESTFEGLNETKAFNNLAVSGLYPPAS
                     TFKAITYTAKLEENLPFPTDVEGVDGDLIHCDGTLVLDELADGSAQVKHDWYDPRNLG
                     WLDIHGALSNSCNIYFWNVALGTYTAYQGQAKENILQDWATRVGYGHETGIDLTSEAP
                     GIIPTRQLFEEWKEYQLENPDEPPRLEPARLELASPWLGGDLMDFAIGQGAFTATPLQ
                     VASSYAVLVNGGKVMEPRVVRETIDNEGNVTPVERPGPVTVDIDPATRASLLEDLGGV
                     VNGGTAAAAFSDFGEGVENVGGKTGTAQVSATRDNHAWFVGVAPLDDPKYVVAILIDE
                     GGSGGQVAAPVARHIMQYLTGNEPTPIVEGEEAD"
     gene            1978..>2252
                     /locus_tag="VFU96_09505"
     CDS             1978..>2252
                     /locus_tag="VFU96_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113544.1"
                     /translation="MAVLTRLREVAVNRGRNKSDRILFGTMLALSGFGLLMIYSATSS
                     AGSVGMERQMIFVAAGLTVYAIISNIDYRELKNLIPIVSILVLLGLFA"
BASE COUNT          429 a          708 c          720 g          395 t
ORIGIN      
        1 gaaccgctct cagataccca tgaagttcgc gctgagactc acgactctgg gcgtcatctt
       61 caccgtgatg ttctcggtga tcgggttgcg tctgtggttc atccaggttg cggagggccc
      121 ccagatcgcc cgagccgccg aagaggcgac ctgggtgggc aagaccacat acgccgcacg
      181 gggcgacatc tacgatcgga atgggactct cctcgccacc agccggatgg tccccgccgt
      241 aatggtcgac cgtaccttcg tccaacccga ccagagagag cccttgatcc aggagctggc
      301 ctccatcgtc ggggtcgacc cagttcaact cgatgacatg tacgaggagg ccgggatcaa
      361 cgggcgcttc caggtgagca cggtctccaa cgagaccgcc tatgcgataa acgagtcgct
      421 cgatgagctc cccggggtag aggtggtcaa ggtcccggaa cgggtctatc tcgccgggcc
      481 cacgatggct cacgtcatcg gtcatctcgg cttgcccgaa gaggcagatc tggccgagcg
      541 tgaggacctg gaaccagatg tccgcatcgg gaagctgggg gtggagaggg tgtacgacga
      601 ggtgctgcaa ggcaccggcg gctcggtgga gtaccgcgtg agacgtggcg agatcatcga
      661 acaacgccca ccggtcgacc cggtcaccgg cagctcgctc gtgctcaatc tcgacctcgc
      721 cctccaggag caggtcgagc tcgctctgga gagtggtgtc gccctatcca acgaggtcaa
      781 agaggaggag agagcagacg gggaggaggt cttcaacgag actcagcggg ccgcggcagt
      841 cgtgctcgat gtcgacacct tcgatgtgct cgccttggct tcgttccccg atttcgatcc
      901 tcagctcttc gtggccggga tcgacgaatc caccttcgag ggcctcaacg agaccaaggc
      961 cttcaacaac ctcgccgtca gcggcctcta tccaccggcc tcgaccttca aggcgatcac
     1021 ctacacggcc aagctcgaag agaacctccc gttcccaaca gatgtggagg gggtcgacgg
     1081 cgacctgatc cattgtgacg ggaccctggt cctcgacgag ctcgcggatg gctcggccca
     1141 ggtcaagcac gactggtacg acccccgcaa cctcggctgg ctcgacatcc atggggcact
     1201 gtcgaattct tgcaacatct acttctggaa cgtcgcgctc ggcacctaca ccgcctacca
     1261 ggggcaggcc aaggagaaca tcctccagga ctgggcgacc cgggtgggat atggccacga
     1321 gaccggcatc gacctgacca gtgaggctcc cggcatcatc cctacgcggc agctcttcga
     1381 agagtggaag gaataccaat tggagaaccc ggatgagccg cctcgtctcg agccggcccg
     1441 tctcgagttg gcctcgccat ggctcggcgg tgacctgatg gacttcgcca tcggacaggg
     1501 agcattcacc gccacgcccc ttcaggtcgc ctcctcctat gcggtgctgg tcaatggcgg
     1561 gaaggtcatg gagccgagag tggtcaggga gacaatcgac aatgagggga acgtcacccc
     1621 ggtcgagcgc ccgggtccag tgaccgtcga catcgatccg gcgacgagag catccctgct
     1681 cgaggacctg ggtggcgtcg tgaacggggg gacggctgcc gccgctttca gcgatttcgg
     1741 tgaaggcgtg gagaacgtcg gagggaagac cgggaccgct caggtgtcag ccacccggga
     1801 caatcacgcc tggttcgtgg gtgtcgcccc tctcgacgac cccaagtacg tggtggcgat
     1861 cctcatcgac gaaggtgggt ccggggggca ggtggcagca ccggtggccc gtcacatcat
     1921 gcagtatctg accgggaacg agccgacacc cattgtcgaa ggtgaggaag ccgactgatg
     1981 gcagtcctga cccgccttcg cgaagtggct gtcaaccggg gccgcaacaa gtcggaccgc
     2041 atcctgttcg ggaccatgct cgccctctct ggcttcgggc tgctgatgat ctactcggcg
     2101 accagtagcg ccgggtccgt cggcatggag cgccagatga tcttcgtggc ggccgggctg
     2161 accgtttacg ccatcatctc caacatcgac taccgggagt tgaagaacct gatcccgatc
     2221 gtgtcgatcc tggtgcttct cgggctgttc gc
//
LOCUS       DASTXO010000452         4559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_63396, whole genome shotgun sequence.
ACCESSION   DASTXO010000452 DASTXO010000000
VERSION     DASTXO010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63396"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..744)
                     /locus_tag="VFU96_09510"
     CDS             complement(49..744)
                     /locus_tag="VFU96_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113545.1"
                     /translation="MDSALALVAALAASLFALDLWLDYRRRPRPHIAAYGIGMTMFAV
                     ATWAFFLGVTVGWTGPMYRVFYLFGAILNIPFLALGSMFLVVGRRSGHIMAIALGALT
                     AISTTLTATVPFANPLPETGVPHDVFASGFGPRLFAIIGGAVGATILAVLSLVSIFRF
                     WKKNRRIVWGNLLILGGTLAAASGGTGLALGESSAFALSLLVAVTLIWAGYRVASGGR
                     RAPASNDPALAGL"
     gene            complement(764..1786)
                     /locus_tag="VFU96_09515"
     CDS             complement(764..1786)
                     /locus_tag="VFU96_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5113546.1"
                     /translation="MSSATDRLAIRARDLTRSFGDAVALDHFSLDVWEGGVVTILGPS
                     GCGKTTALRVIAGFENAGGLVEIHGRTVLGPGVHVPPERRRVGMVFQDYALFPHMTVA
                     GNVAYGLPRGDREPRVGEVLELVGLTGLESRMPNELSGGQQQRVALARALAPKPEVIL
                     LDEPFSNLDASLRDRVRRELRTILTEARTTAVFVTHDQEEALATSDIVAVMREGRVLQ
                     ADTPGHLYSNPADRWVAEFLGDADVLESVAKDGRVDTPVGAFDTDIHGRVSVIVRPEN
                     VQISLGETPNAVVAGSEFFGHDQLVTLALHEGTRVRARIGPRPIYRVGQPARVRATDA
                     RIFARG"
     gene            complement(1770..3257)
                     /locus_tag="VFU96_09520"
     CDS             complement(1770..3257)
                     /locus_tag="VFU96_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU5113547.1"
                     /translation="MALLPALIPPISLLFQVIGTGTTLAVPPARLAELMFNTVVLTVA
                     VTVTAVAIGLATAWTTTRVDLPFRKGWMTLAALPLVVPSYVAALTLIAATGPSGVLVE
                     IPTPYGFVGSWLVLSVFTAPLVHLTLIPGLRAIDPATEESATGLGANRWRVFATVTLP
                     QLKPALVSSGLLVGLYTISDFGAVSLLRYDTFTRAIYTLYRGQIDRRPSATLSLILMA
                     LAIVILLIERRYRARVALHSRRPRRARPLLSLMGWPRAASILSLALYTLVALGLPLAV
                     MAITVIGGLEADQIVGSLGAEAVRSLGVALVAALVTVILAFPVAMVTTRRPGRLSGWV
                     ESTMWGAYSLPHITVGVAAVGFALTWARPLYQTLVYLVIIYGAIFLPQAVGAAQDSLM
                     RASPDLEDASRGLGRGPVATLMRVTVPLAIPGLVAGAALVFLSVMKELPATLLLRPNG
                     FETLAIRIWSSTAEGFITRASAASLVLLAVSIVPLFLVMSRELSD"
     gene            complement(3346..4326)
                     /locus_tag="VFU96_09525"
     CDS             complement(3346..4326)
                     /locus_tag="VFU96_09525"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU5113548.1"
                     /translation="MTWVLAVAMVSTACGGSTAETDEPLVIYSGRTEELVGPLLEQFE
                     SESGIDVEIRYADSSELAATLLAEGEETEADVFFAQDPASLGVVADLMSQLPEETLSL
                     VDARLRDGDGRWVGTSGRVRVFVHNTGSDLPLPQTIDDAVDPAWAGQLGVAPTNGSFL
                     AFVSAMILERGEDETREWLEALAANDPVEFDGNAPIVEATDTGELGGGLVNHYYLLRL
                     QAEGQGENTENYFIPAGDVGSLVMPAGVGVLAATQQPEAAQQFVDFLLAEEAQTYFAT
                     ETFEFPLIEGVPQPEGVPPLDEIAAPDIDLSELAGVLDLATSLVAEAGLV"
BASE COUNT          873 a         1501 c         1528 g          657 t
ORIGIN      
        1 tcgactcggc ccttggcaag gtacatcccg gtgagcctca gccgaagatc agagcccggc
       61 cagggccggg tcgttcgagg ctggggccct tcgaccgccc gatgccaccc gatagccagc
      121 ccagatcagc gtgaccgcca ccaagaggga gagggcgaat gcgctcgact caccgagggc
      181 gagaccggtg ccgccggacg ccgccgccag ggtcccgccc aggatgagca ggttgcccca
      241 gacgatccgg cgattcttct tccagaaccg aaagatcgac accagcgaca ggacagcgag
      301 gatcgtggcc ccgaccgccc cgccgatgat ggcgaacagt cggggcccga atccagaggc
      361 gaagacatcg tgtggcactc cggtctcagg tagaggattg gcgaacggca ccgtcgcggt
      421 gagggtcgtg gagatggcgg tgagagcacc cagagcaatg gccatgatgt ggcccgagcg
      481 cctgccgacg accaggaaca tcgagccgag tgccaggaac gggatgttga ggatggcccc
      541 gaacagatag aagacgcggt acatcggacc ggtccagccc acggtgacac ccaggaagaa
      601 cgcccatgtg gcgaccgcga acatggtcat gccgatcccg taggcggcga tgtggggccg
      661 gggccgccgt cggtaatcga gccacagatc gagtgcgaac agggaagcgg ccaacgccgc
      721 taccagggca agtgccgaat ccaccggcgt caggctagga ggatcaaccc ctggcgaaga
      781 tgcgggcgtc ggtcgcccgc actcgcgccg gctggccgac gcggtagatg ggacggggcc
      841 cgatccgggc tcgaacccgg gtgccttcgt gcaatgcgag ggtcaccagt tggtcatggc
      901 cgaagaactc ggacccggcc acgacagcgt tcggggtctc accgagtgag atctgcacat
      961 tctccggccg gacgatcacg gaaacgcggc cgtggatatc ggtgtcgaag gctccgaccg
     1021 gggtgtcgac gcgcccatcc ttcgccaccg actcgaggac gtcggcatca ccgaggaact
     1081 cggcgaccca tcggtcggcc gggttcgagt agagatgacc gggtgtatcg gcctgcagga
     1141 cccggccctc cctcatcacg gccacgatgt cggatgtggc cagcgcctcc tcttggtcat
     1201 gggtgacgaa gacagcggtg gtgcgcgcct cggtcaggat ggttcgcagc tcgcgccgca
     1261 cccggtcacg caggctggca tccaggttgc tgaaaggctc gtcgagcagg atcacctccg
     1321 gtttcggggc cagggcgcgg gccagggcga cgcgctgctg ttggcctccg gagagctcgt
     1381 tcggcatccg gctctccaac ccggtcaaac cgaccagttc tagaacctcg ccaactcgtg
     1441 gttcccggtc cccccgcggg agcccgtagg cgacattccc cgcgactgtc atatgaggga
     1501 acagggcgta gtcctgaaag accatgccca ccctgcgccg ctccgggggg acgtggaccc
     1561 cgggccccag cacggtgcgg ccgtggatct ccaccaaccc gcctgcattc tcaaaacccg
     1621 cgatcacccg caacgcagtg gtcttgccac accccgacgg gccgaggatg gtgaccaccc
     1681 cgccctccca gacgtcgagg ctgaagtgat cgagggcgac cgcgtcgccg aaggacctgg
     1741 tcagatcccg agcccggatg gcgaggcggt cagtcgctga gctcacgtga catcacgagg
     1801 aagagtggca cgatggaaac ggcgaggagg accaggctgg ccgcggatgc cctggtgatg
     1861 aagccctcgg ctgtggagct ccagatcctg atcgccaatg tctcgaaccc gtttgggcgc
     1921 agaaggagcg tcgccgggag ctctttcatg acgctgagaa acaccagcgc ggcgcccgcc
     1981 accaatcccg ggatggccag tgggaccgtt acccgcatca gcgtcgccac cggtccccga
     2041 cccagcccgc gtgaggcgtc ttcgaggtcg gggctggccc tcatgagcga gtcctgggct
     2101 gccccgaccg cctgcgggag gaagatggct ccgtagatga tgaccaggta gaccaacgtc
     2161 tgatagaggg gccgagccca ggtcaaggcg aagccgaccg ccgccacacc cacggtgatg
     2221 tgggggaggg aataggcgcc ccacatggtc gactccaccc agcccgagag gcgccccggc
     2281 cgccgggtgg tcaccatcgc caccgggaag gcgaggatca cggtcaccaa ggcggcgacg
     2341 agggccactc ccaatgatcg aactgcctcc gcgcccagcg accccacgat ctgatcggct
     2401 tcgagccccc cgatgacagt gattgccatg accgcaagcg gaagaccgag tgcgaccagc
     2461 gtgtagaggg ccagcgacag gatcgaagcg gcacgtggcc agcccatcaa ggagagcaaa
     2521 ggacgcgccc ggcgcggccg gcgggagtga agggcgaccc tggcacggta tcggcgctcg
     2581 atgaggagga tgacgatcgc cagggccatg aggatcaacg acaaggtcgc cgatggcctc
     2641 cggtcgatct gccccctgta gagggtgtag atggccctcg tgaacgtgtc gtagcgaagc
     2701 agcgatacgg ctccgaaatc tgagatggtg tagagcccca cgaggagacc ggatgagacc
     2761 agcgccggct tcaactgggg aagggtcacc gtggcgaaca cccgccaccg attcgctccc
     2821 aaccctgttg ccgactcttc ggttgccgga tcgatggccc gcagccccgg gatgagggtg
     2881 aggtggacca ggggggccgt gaacaccgag agcaccagcc atgagccgac gaacccgtac
     2941 ggcgtcggga tctcgaccag gactcccgaa ggcccggttg cggcaatcag ggtcaaggcc
     3001 gcgacatatg acgggacaac tagagggaga gcggcgagcg tcatccaccc tttgcgaaac
     3061 ggaagatcga ctctcgtcgt cgtccaggca gtggccagcc caatggcgac tgccgtgact
     3121 gtcacggcga cagtgagcac gacggtgttg aacatgagct cggcgagccg ggccgggggc
     3181 accgccagtg tcgtcccggt cccgatgacc tggaacaaga ggcttatcgg aggaatgagt
     3241 gcggggagga gggccactcc cgccgctagc caccaccccc acccgccagg atgccgagcc
     3301 ggcccgacct cgggccggcg tgcggtcagc ggcccggctg tcaactcaca ccagaccagc
     3361 ctcggccacg aggctggtgg ccaggtcgag cacgcccgcc agctcgctca ggtcgatgtc
     3421 gggggctgcg atctcgtcca gcggtggcac tccttcgggc tggggcaccc cttcgatcaa
     3481 gggaaactcg aatgtctcgg tcgcgaagta ggtctgcgct tcttcggcga gcaggaaatc
     3541 gacgaactgt tgggcagcct cgggctgctg ggtggcggcc aagaccccga ctccggccgg
     3601 catgaccaac gagccgacgt ccccggccgg aatgaagtag ttctcggtgt tctcgccctg
     3661 accctcagcc tgcaagcgga ggaggtagta atggttgacc aggccacctc ccagctcacc
     3721 ggtgtcggtg gcctcgacga taggggcgtt cccatcgaat tcgaccgggt cattcgcggc
     3781 gagagcctcc agccattctc gtgtctcgtc ctccccgcgc tcgaggatca tggcggacac
     3841 gaaggcgagg aacgaaccgt tggttggcgc cacgccgagc tgaccggccc atgccgggtc
     3901 gactgcatca tcgatggtct gggggagtgg caagtccgag ccggtgttgt gcacgaagac
     3961 cctcacccgc cctgaggtcc cgacccaacg gccatctcca tcgcggaggc gcgcgtcgac
     4021 cagcgagagc gtctcctcgg gaagctggct catcaggtct gccacgacgc caagtgacgc
     4081 agggtcttgg gcaaagaaca catcggcctc ggtctcctcc ccctcggcga ggagggtcgc
     4141 ggcgagctcg ctcgaatcgg cgtacctgat ctcgacgtcg atccccgatt cgctctcgaa
     4201 ctgctcgagg agggggccga ccaactcttc ggttcgaccc gaatagatca ccagcggctc
     4261 atccgtctcc gcagtgctgc cgccgcacgc agtcgacacc atcgccacgg ccaggaccca
     4321 agtcatcacc cttcgcaacg tctgcctcca gatcgttagg tacctaacaa tgtgggaatc
     4381 taattaacac tatggccgta gtgcaaagga gagcgagaca ggtggtcgtc tggccccggc
     4441 tgacaggctg gagtgcccag cctcagggcc gcaaacggct ctcgaggtgc cgaccgcgcg
     4501 gcgtagtcgg ccgccgggcc cgctcccgct cctgcatcca ggtagagacc gcttcgaga
//
LOCUS       DASTXO010000453         3155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_911003, whole genome shotgun sequence.
ACCESSION   DASTXO010000453 DASTXO010000000
VERSION     DASTXO010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911003"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..981
                     /locus_tag="VFU96_09530"
     CDS             247..981
                     /locus_tag="VFU96_09530"
                     /inference="COORDINATES: protein motif:HMM:NF039623.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5995 family protein"
                     /protein_id="HEU5113549.1"
                     /translation="MVEAIADVVQGAKDSESRVGFFAAMYSGVTLQVRAGVESDLFED
                     GARMERLATTFATRYLDAVNRFRSGDQPSRCWDFAFRSAEMWRPLILQHLLLGINAHI
                     NLDLGIAAALTSPGDDLPDLRNDFVEINRVLARQVDGIRETIGKLSPWIGLLDRVDPS
                     AGRAIVNFSIERARDQAWTVAELLAGLPEERWPEHVDVLDRNALSLARLVRDPPGVIL
                     KAGLRIIRVRETNDVRRAIERLERPA"
     gene            complement(1063..2409)
                     /locus_tag="VFU96_09535"
     CDS             complement(1063..2409)
                     /locus_tag="VFU96_09535"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5113550.1"
                     /translation="MEGIPKTRYTQNDGVNLAYQVVGNGDVDLLLVDTWVHHVEAIWD
                     VPDAARFLRRLGAFGRLIHFDRRGTGLSDSVPLDRLPDLETQVGDAVAVLDAAGSKRA
                     VVIGLNDGTIIASLLAIEYPERCHSLVLWTLTSVHALPETLQMTSVDDVVEMIRADAE
                     HDRSGVDLLAPSRAGDSRFDEDLARLQRFSVRVGAFAHYYRQTMDVDLTTVLPSIRCP
                     TLVLNRTGDRIVPVEQSRNAAALIDSARFVELSGTDHLIFSEGIDEVAEEIEEFITGS
                     RTAVDPDRMLTTLLFTDIVGSTDQAEIIGDRRWRDLLDMHNDLTRRELARFGGREVST
                     TGDGFFAVFDRPVSGVRCALSLVSSMPSLGVQVRTGVHTGEVEVRGSDFGGMAVHIAS
                     RIASLAEPGEVLVSSTVRDLLVGSDLGFDDRGVHVLKGVTGDWHVYGASTGVAGPV"
     gene            complement(2442..2514)
                     /locus_tag="VFU96_09540"
     tRNA            complement(2442..2514)
                     /locus_tag="VFU96_09540"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2479..2481),aa:Lys,seq:ttt)
     gene            2610..>3155
                     /locus_tag="VFU96_09545"
     CDS             2610..>3155
                     /locus_tag="VFU96_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113551.1"
                     /translation="MEARILAAHGEVLVMSDDEGITFELAGRKVDLRWPDITGAGLAF
                     QPGADVTFPVDSIELPGGRKVPFQMVPFGGKLTGLARRLAATHRALLIGDGGNGFQVT
                     LPIDDPGTETVIAELRTRLGDRWHHDTQDMEELRKDLRMRTSWSGRLVGLLFVILVGI
                     GGFLAIAAWAGVKAFWDEGDLS"
BASE COUNT          600 a          973 c          999 g          583 t
ORIGIN      
        1 ggtcgcatcg aggtctatga gttcaacgag cacgagggag gcggttcact ccacttcgag
       61 aagcaggtcg cgttcgtccg cgagcacttg ggctgagctg gtccggagtg ccctcggtgg
      121 gaatgcggtc ctggtctcgg cttcctcgct ggacccgtgc caccattggg gccgtggagt
      181 cccgtgcagt gctggccaag gacggtcgtc atcggttccg ttgaacatcc gcagaatctg
      241 gacggggtgg tcgaagccat cgccgacgtc gtgcagggag cgaaggactc ggagagtcgt
      301 gtcgggttct ttgcagccat gtacagcggc gtgacgttgc aggttcgagc gggtgtggag
      361 tccgatctat tcgaggacgg cgctcgcatg gagaggttgg ccaccacgtt cgccacccgt
      421 tatctcgacg ccgtgaatcg attccgcagt ggggatcagc caagtaggtg ttgggacttc
      481 gcttttcggt cggcggaaat gtggcggccc ctcatcctcc agcacctcct gttggggata
      541 aatgcgcata tcaacctcga cctcggtatt gctgccgccc tgacctcgcc tggcgatgat
      601 cttccagacc ttcgcaacga cttcgtggag atcaaccgtg tgctggctcg ccaggtagac
      661 ggaatacggg aaacgattgg aaagctctct ccctggatcg ggctcctcga tcgggtcgat
      721 ccaagtgccg gtcgagccat tgtcaatttc agtatcgagc gagcacgaga ccaggcatgg
      781 accgtggccg agcttctcgc cggcctgccg gaggaacggt ggcccgagca cgtcgatgtc
      841 ctggacagga atgccctgtc acttgcccgg ttggtccgcg accctcccgg cgtgatcctc
      901 aaggctggtc tacgcatcat ccgagttcga gaaacgaatg acgtccgccg ggcgatcgag
      961 aggctcgaac ggccggcata gggtggacgc accatcagcg atcacaccgc gagggggtca
     1021 cagctccttg gtgtcggtga tgtcccaacc actctgaacg aatcagacag gtccggcgac
     1081 cccggtcgac gccccataga catgccaatc gccggtgacg cctttgagca cgtggactcc
     1141 tcgatcgtcg aaccccaagt cggacccaac cagcagatct ctcacagtgc tcgagaccag
     1201 cacctcacct ggctcggcga gactggcgat tcgggaggcg atatgcactg ccattccgcc
     1261 aaagtctgag ccacgcacct cgacctcacc ggtatggacg cccgttcgga cctgcactcc
     1321 gagtgacggc atcgacgaga ccaatgaaag agcgcatcga acccccgaga ccggcctatc
     1381 gaagacggcg aagaacccgt ccccagtggt agagacctcg cgtcccccga aacgggccag
     1441 ttcccgacgg gtgaggtcgt tgtgcatgtc gagcaggtca cgccaacgtc gatcaccgat
     1501 gatctctgcc tggtcggtgg acccaacgat gtcggtgaac agcagtgtgg tgagcatgcg
     1561 gtcggggtcc acggcggtgc gtgacccggt gatgaactcc tcgatctcct ctgcaacctc
     1621 gtctatcccc tcggagaaga tgaggtggtc ggtcccggag agctcgacga acctggcgct
     1681 gtcgatcaac gctgctgcgt tcctcgattg ctcgacgggc acaatgcggt ccccagtgcg
     1741 gttcaacacc aacgtggggc atcgaatgga cgggagcacg gtcgtcagat ccacatccat
     1801 cgtctggcgg tagtaatggg cgaacgcccc gactcgaact gagaaacgct ggaggcgggc
     1861 caggtcctcg tcgaacctgg aatcgccggc gcggctcggc gccagcaaat cgacaccgct
     1921 gcgatcgtgc tcggcatcgg ctcggatcat ctccacgaca tcgtccactg acgtcatttg
     1981 gagtgtttcg ggcaacgcgt ggaccgaagt gagagtccag agaaccaacg agtggcatcg
     2041 ttccgggtac tcgatggcga gcaaggaggc gatgatcgtc ccgtcgttga gaccgatcac
     2101 cacggctcgt ttcgacccgg cggcatcgag cacggcgacg gcgtccccca cctgggtctc
     2161 cagatcgggc aggcggtcca gtgggaccga atcggaaagt ccggtgcccc gtcggtcgaa
     2221 atggatcaga cgtccaaacg caccaagccg acgaaggaac cgggccgcgt cgggcacatc
     2281 ccagatcgct tcaacgtggt gtacccatgt gtcgaccagc agcaggtcta catcaccgtt
     2341 ccccaccacc tgataggcaa ggttgacccc gtcgttttgg gtgtaccggg tcttgggaat
     2401 gccctccacc gcgggaggct accgaaaggg cccgatccag gtgggtcccg tgggactcga
     2461 acccacaacc aacgggttaa aagcccgctg ctctaccaat tgagctagag acccggagcg
     2521 gacaaagggt accgggggcc gtcggaaatc ctgccggatc cgcccacctc tctcatcgaa
     2581 ctttcagggc cttgtcgtac gttcggcgag tggaggcacg gatattggcg gcacacggtg
     2641 aggttctcgt catgtccgac gacgagggga tcacgttcga actcgcgggt aggaaggtcg
     2701 atctccgttg gcccgatatc actggcgcgg gcctcgcctt tcaacctgga gcggatgtca
     2761 ccttcccggt ggactcgatc gaacttcccg gtgggcggaa ggtgccgttc cagatggtcc
     2821 cgtttggcgg caaactcacc gggctcgccc gccggctggc cgccacacac cgcgccctcc
     2881 tgataggaga cggtgggaac ggctttcagg tcacgcttcc catcgacgac cccggcaccg
     2941 aaaccgtcat tgccgagctc aggacccgcc tcggggaccg atggcatcac gacacgcagg
     3001 acatggaaga acttcgcaag gaccttcgaa tgcggacatc gtggagcggc cggctcgtcg
     3061 gtctcttgtt cgtcatcctc gtggggatcg gtgggtttct ggcgatcgcg gcctgggccg
     3121 gtgtcaaggc attctgggat gaaggtgact tgtcg
//
LOCUS       DASTXO010000454         3632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_506156, whole genome shotgun sequence.
ACCESSION   DASTXO010000454 DASTXO010000000
VERSION     DASTXO010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506156"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VFU96_09550"
     CDS             complement(<1..370)
                     /locus_tag="VFU96_09550"
                     /inference="COORDINATES: protein motif:HMM:NF017949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5113552.1"
                     /translation="MATSDPIAEIIGDYRAFAAMQRDRLRARGIDITPYPLSHLAVRV
                     ADWDLYVHQRTLLERHAKTNRESVWNGRPISLLVLAEPLDVLDGTPVSRIELIPPVHQ
                     RVYRMGLEHLGVVVGEEIDEF"
     gene            550..837
                     /locus_tag="VFU96_09555"
     CDS             550..837
                     /locus_tag="VFU96_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113553.1"
                     /translation="MAVIVRIVIDSATQDDHDRLGDAVETRLQDQGGPPDGLMVHLGW
                     PADGGFVIIEAWRTEELFRSYQEQLLGPALREAGLAAREPEIGPAWSIARP"
     gene            complement(1055..2377)
                     /locus_tag="VFU96_09560"
     CDS             complement(1055..2377)
                     /locus_tag="VFU96_09560"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEU5113554.1"
                     /translation="MAAQSGDNVLHSEVEPWIASSNVDRSGDGVPDLIAGYQQDRWSN
                     GGARGVFASVLHGGAWIQVAIPGVSTCLGGTELRATDPWVTISPDGTAYFMTLSIGDP
                     DVDPTSVMYVNRSTNGGLTWGPPIELIRDATPFLFNDKNSMTADPFDSRFVYAIWDRS
                     RFPSDVREFHSLAGFPASLRSDATFTRTTDGGATWEPARAIFQPRANQFGIGHQIVVL
                     SDGTLVDGFMLFHGSGRNKKGQEIAVMISQDRGETWTEPIRVAKALPGFVSDPDDGFP
                     LRTGDIIPEIAAGPNGSVYMVWQEATLAPSGSAIAFSKSLDGGLTWSEPVRINSRPDV
                     QAFTPSVEVLPDGTIAVTHYDLRNNTPDPGTLPTDYWFLHSHDGGTTWTETRVTPTSF
                     DHELAPIARGYFLGDYMGLAVRTGGFMAAFTQTEATDPASIFVRRLIP"
     gene            2983..3555
                     /locus_tag="VFU96_09565"
     CDS             2983..3555
                     /locus_tag="VFU96_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113555.1"
                     /translation="MRLSRTLLLTAGLAVAVSMSTAGLAAAQPIEQEHFHDSGSEVIE
                     EFCGDLTVRHDFEADGYFSATPHGPDGLIYFADRFRLTDAWTNLANDKTFTVTFAGQQ
                     KDQQVTDNGDGTLTIQVLIAGRNFRYGPDGTRLSVDAGIFRFQFLVDHGGTPTDPDDD
                     EEIEGSFEVLQDTAGRAGIDFCDVIHDIIG"
BASE COUNT          637 a         1180 c         1216 g          599 t
ORIGIN      
        1 agaactcgtc gatctcctca ccgacgacga cgccgaggtg ctcgaggccc atccggtaga
       61 cacgctggtg aacgggcggg atgagctcga tgcgcgagac cggagtccca tcgaggacat
      121 cgagcggctc ggcgagcacg agcaaggaga tcggacgccc gttccagacg ctctcgcggt
      181 tcgtcttcgc atggcgctcg agaagcgtcc gctgatggac gtacaggtcc cagtcggcga
      241 ctcggacggc gagatggctg agcggatacg gcgtgatgtc gatcccgcgg gctcgaagac
      301 ggtcgcgctg catcgccgcg aaggctcgat agtccccgat gatctcggcg atcggatcag
      361 acgtcgccat gtggcatcct cctccgccat cgtacgagat aggggacacg acatagctgc
      421 cgctcggcgt gcccggggtc ctgtgccatc cgcctcgcag cggggtgcgc tcagttcggg
      481 caatcggcac gcgcctcgcg ccgcctaccc ggcgataggt ggccggtccc gaccgagtag
      541 cgtccgggga tggcagtgat cgtccggatc gtcatcgact cggcgactca ggatgatcac
      601 gaccgtttgg gggatgcagt tgagactcgg ctgcaggatc aaggcggccc cccggacggg
      661 ctcatggtgc atctcggttg gccggcggac ggcgggttcg tgatcatcga ggcgtggaga
      721 accgaggagc tcttccgctc gtaccaggag cagttgctcg ggcctgcact tcgcgaggct
      781 ggtcttgctg ccagagagcc tgagatcggc ccggcgtggt cgatcgctcg tccgtagagg
      841 actgcctcac atgtgccgat cggggacgca ggacggcgcc atatctgccg atctcgcgcc
      901 gcgtcgttat tcgctgtcaa tcggatcggc gacggccgcc caccgcgggc aatcggtgcg
      961 ggtgcaggac tccgtggcca tcgacccgtg aggcctttcc tgtgggacgg aggggaacct
     1021 ccgccccaca ggctcgattc gaagatgtcc ctgtctacgg gatgagtcgt cgcacgaaga
     1081 tgctggcggg atcggtggcc tctgtctggg tgaaggctgc catgaagcca ccggtgcgca
     1141 ccgccagacc catgtaatcg ccgaggaagt atccgcgggc gatgggtgcc agctcgtgat
     1201 cgaacgacgt cggggtgacc cgcgtctcag tccacgtggt gccgccatcg tgcgagtgca
     1261 ggaaccagta atcggtcggc agcgtgcctg gatctggcgt gttgttccgc aggtcgtagt
     1321 gcgtgacggc gatggtcccg tcggggagca cctcgaccga cggtgtgaac gcctgcacgt
     1381 ccggccggga gttgatgcgc accggctcgg accaagtgag accaccgtcc agtgacttcg
     1441 agaaagcgat cgccgacccg gacggcgcca gcgtcgcctc ctgccaaacc atgtacaccg
     1501 acccgttcgg gccggcggcg atctccggga tgatgtcgcc cgtacgtagc gggaaaccgt
     1561 cgtcggggtc gctcacgaaa ccgggcagcg ctttcgcgac gcggattggc tcggtccagg
     1621 tctcgccgcg gtcctgagag atcatcaccg cgatctcctg gcccttcttg tttctcccgg
     1681 agccgtggaa gagcatgaac ccgtcaacca gggtgccgtc ggacagcacg acgatctggt
     1741 gcccgatccc gaactggttg gcgcggggtt ggaagatggc ccgggccggc tcccaggtgg
     1801 cgccgccgtc ggtggtccgg gtgaacgtcg cgtcgctgcg gagcgaggcg gggaacccgg
     1861 ccagggagtg gaactcgcgg acgtcactgg ggaagcggct gcggtcccag atcgcgtaga
     1921 cgaacctcga gtcgaacggg tcggcggtca tcgagttctt gtcgttgaac aggaacggcg
     1981 tggcgtcccg gatcagttcg atgggtggcc cccacgtcag accgccgttc gtggatcggt
     2041 tgacgtacat caccgaggtc gggtccacgt cgggatcccc gatcgacagc gtcatgaagt
     2101 acgccgtccc gtcgggggag atcgtgaccc acgggtcggt ggcacggagc tccgtgcccc
     2161 ctaggcacgt cgacaccccg gggatcgcca cctggatcca ggcgccgcca tgaaggacac
     2221 tggcgaagac gcctcgggcc ccgccgttcg accagcgatc ctgctggtag ccagcgatca
     2281 ggtctggaac accatcccca ctgcggtcga cgttgctcga cgcgatccac ggctcgacct
     2341 cgctgtgcag gacgttgtcg ccgctctggg cagccacgtc atcggcagtg caccccgcga
     2401 gagggctcgt gcccgaaacc ttgacaagag gccccagggt cccaggcccg gcccccaccg
     2461 gacccgcaaa cagaaacagc gcggccgcgg ccgcgactac agcgcgttga caggagcgag
     2521 acatgcgtgg accccctcac gtagcaccac gaaaaccatt cccgatgcga caccacttgt
     2581 cggtttacgc cccccacctg aaacgatgct gaagctacgc cggcgtccag cggtgtcccg
     2641 gaagggcctc caccagacgc agagggttca gctttggcga tgggccgatc gggcctgaag
     2701 acgcgccata tctgccgcta tgcgcccaca ggtgagcgcc ggatcgggca ttacgcgctg
     2761 cccggatgac ccgacttccc aggacattgg gcataccttg ggcgccggac aacccccgtt
     2821 gtccacaagc gtgggcgtct cgacccgtca tcgggacgcc cacacccaag ggcggcgcta
     2881 tgctctcggc ggcaaaggag cgatgcacgg gtttgtacag ggcgggctgt gccgagccgg
     2941 gcggactcga cgggttccac atcggcggat aggaggcggg ccatgagact gtcgcgcaca
     3001 cttctgttga cggcggggtt agccgtcgcg gtctcgatgt cgaccgcagg tctggccgcg
     3061 gcacaaccga ttgaacaaga gcatttccac gattcgggca gcgaggtcat cgaggagttc
     3121 tgcggcgacc tgaccgtccg ccacgatttc gaggccgacg ggtacttctc ggccacgccg
     3181 cacggcccgg atggtctgat ctacttcgca gaccgcttcc gtctaaccga tgcgtggacc
     3241 aacctggcca acgacaagac cttcaccgtc acctttgctg gccaacaaaa agaccagcag
     3301 gtgaccgaca acggtgacgg cacgctgacg atccaggtcc tcatagctgg gcgtaatttc
     3361 agatacgggc ccgacggcac cagattgtcc gtggatgccg gaatcttccg gttccagttc
     3421 ctggtcgacc atggtgggac gcccaccgac cccgacgacg acgaggagat cgagggttcc
     3481 ttcgaagtcc tccaggacac cgcaggccgc gcaggcatcg acttctgcga cgtcatccac
     3541 gacatcatcg gctgacaaca ccgcgtgccg gcatcgccca cccggcgggt gaggtttccg
     3601 cgccctctag ccaaagtggc gaccacatgc gg
//
LOCUS       DASTXO010000455         4115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1095439, whole genome shotgun sequence.
ACCESSION   DASTXO010000455 DASTXO010000000
VERSION     DASTXO010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095439"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VFU96_09570"
     CDS             complement(<1..383)
                     /locus_tag="VFU96_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019067430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HEU5113556.1"
                     /translation="MSTPLEDYAVIGDTETAALVSTGGSIDWLCLPRFDSGSSLAALL
                     GDEENGRWRLAPAGGPSRVRRQYRPGTLVLETEMEAPEGTVRLIDVMPIRGREGGDNA
                     DVVRIVEGVSGRVEMRMDLTIRFDYG"
     gene            complement(394..789)
                     /locus_tag="VFU96_09575"
     CDS             complement(394..789)
                     /locus_tag="VFU96_09575"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5113557.1"
                     /translation="MVIDVGTWVDGYARAWVEMDAEAAAALFSEDANYRSFIFDEPHV
                     GRAGVRDYWSEVTSTQSDVQVRMGRPIVEGRRAAVEFWTNRQNEGSEVTLVGCLLLTF
                     DDDGLCQSLDEYYEFAPGRMDPPEPWGAL"
     gene            860..2437
                     /locus_tag="VFU96_09580"
     CDS             860..2437
                     /locus_tag="VFU96_09580"
                     /EC_number="1.2.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF006916.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinic semialdehyde dehydrogenase"
                     /protein_id="HEU5113558.1"
                     /translation="MASSDPAPLTPHLGMAVLRELEARARRVGREQVEVVAPFTLEVI
                     GRVPQATNDDVADAAARARAAQPAWESRGAADRARILLEFHDILLDQIDEVTDLIQLE
                     GGKSRMDAWREVVDVIGVTRYIANVAPRATRRRRHQGAMPVFTKTYEYRHPKGLVGFI
                     SPWNYPFILAISDAIAALATGNAVLIKPDEKTPFSALLGARLLEEAGVPGDVVQILTG
                     SGAPIGKAIVDSVDYVMFTGSTEVGRSIAEMAGRRLIGCSMELGGKNAAIVLPDADLT
                     RTVRELASASFANAGQTCVAMERIYAHESIREELTRRLAEHTAALPVSAGFDFSSEIS
                     SMVDQGHLDRVHAHVEDAVAKGATLLTGGKPRPDVGPLFYAPTVLTDVTEEMELCREE
                     TFGPVTAIYGYTDIAEAVARANDSEFGLHFSLWTRDPDRARDIAIRLEAGSVTINDGL
                     VASWGSHEAPMGGMKSSGLGRRHGLEGILKFTEPQTVATQRLIPAYSPFGGITPERYT
                     RLVEFLTTVFKRLPFYK"
     gene            complement(2475..2954)
                     /locus_tag="VFU96_09585"
     CDS             complement(2475..2954)
                     /locus_tag="VFU96_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113559.1"
                     /translation="MTDPVEKPDPAELEAWHHTFAPLAFNEAWALLDGTEPTREQEEE
                     MLAATFAQRYHWYQVGTPRNQAISDWQISRVAAVLGYADLALRFGERSLTVCLDNDLD
                     PFVTGFAHEAIARAAADVDDIDMFTEHLEAAKEKLLEIEDPEDRDVLEADLTEMSER"
     gene            complement(2951..>4115)
                     /locus_tag="VFU96_09590"
     CDS             complement(2951..>4115)
                     /locus_tag="VFU96_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020133872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5113560.1"
                     /translation="GHVDLVELETRLVEHSHRPLIIGSFSAASNVTGIISDTAAISRL
                     LHAHGALTFWDFAASAPYVDIEMGSSADGNGEYKDAIFLSPHKLIGGPGTPGVLVVRR
                     DLLRNEVPVVPGGGTVQYVAPDHHIYLSDPEHREEGGTPAIIESIRAGLAFRLKETVG
                     VDTIRALEHEFIRRAIESWSLNPNIEILGNPNAERLSIVSFVIRHGERTLHHNFVVAL
                     LNDLFGIQARGGCSCAGPYGHRLLGIDLDTSSRIEDAVSAGCEVIKPGWVRVNFNYFI
                     AEDEFAYLIEAVHLVADQGWRLLPMYTYEPETALWRHRDGLPKPPASLRDLDFAGGGI
                     EAPMTAPLSELGAYLAEAKRILAEEPEPGGVSPDLGLAGERLRWFPNPAEIER"
BASE COUNT          731 a         1334 c         1330 g          720 t
ORIGIN      
        1 ccgtagtcga agcggatggt gaggtccata cgcatctcca cgcggcctga gacgccctcc
       61 acgattcgga cgacatcggc gttgtcgcca ccctctcgcc ctcggatcgg catgacgtcg
      121 atgagcctga ccgtcccttc cggggcctcc atctcggtct ccagcacgag tgtcccgggg
      181 cggtactgcc tacgcacccg tgaaggccct ccagccggcg ccagtcgcca gcgtccgttc
      241 tcctcgtccc cgagcaatgc agcaagagaa gagccggagt cgaagcgggg gaggcagagc
      301 cagtcgatcg atccccctgt cgagaccagt gccgcggtct ccgtgtcgcc gatgaccgca
      361 tagtcctcta gcggcgtgct caccatggtc ccgctagagg gcaccccagg gctcgggtgg
      421 atccatccga cccggcgcga actcgtagta ctcgtcgagg ctctggcaga gcccgtcgtc
      481 gtcgaaggtc agaaggaggc acccgaccag ggtgacctcc gacccttcgt tttgcctgtt
      541 ggtccagaac tcgactgcgg cgcgccggcc ctcgacgatg ggtcggccca ttctcacctg
      601 cacgtcactc tgggtggagg tcacttcgct ccaatagtcc ctgactccag cgcgaccaac
      661 gtgcggctcg tcgaagatga acgatcggta gttggcatcc tcggagaaaa gtgcagcggc
      721 cgcctcagcg tccatctcca cccaggcgcg ggcgtaccca tccacccatg tcccgacgtc
      781 gatcaccatc tcgattctcc tcacccgtcc ggcgatagct cgaggccagg ctacgtgaga
      841 gggcgggtag cgttgcccca tggcctcgag tgacccggcc ccactcactc cgcaccttgg
      901 catggcggtc ctccgcgagc tggaggcacg ggcccggcgg gtcggccgtg agcaggtcga
      961 ggtcgttgcc ccgttcactc tcgaggtgat tgggcgtgtc ccccaggcca ccaacgacga
     1021 cgttgccgac gccgcggcgc gtgcccgagc tgcccaaccc gcctgggaaa gtcggggggc
     1081 ggcggaccga gcccggattc tcctcgaatt ccacgacatc ctcctcgatc agatcgacga
     1141 ggtcaccgac ctgatccagc tggagggcgg gaaatcccgg atggatgcct ggagagaggt
     1201 cgtcgatgtc atcggggtca ccaggtacat cgccaatgtc gctcctcggg ccaccaggcg
     1261 gcgaagacat cagggtgcga tgccggtgtt caccaagacc tacgagtacc gccatcccaa
     1321 ggggctggtc ggcttcatca gcccctggaa ctacccgttc atactcgcga tcagtgatgc
     1381 gatcgcggcc ctggcgacag gcaacgcggt gctgatcaag cccgacgaga agaccccttt
     1441 ctcggccctg ttgggggcga gactcctcga ggaggcggga gtgcctgggg atgtggtgca
     1501 gatcctcacc gggtccgggg ccccgatcgg gaaggcgatc gtcgattccg tggactacgt
     1561 catgttcacc ggatcgaccg aagtgggccg gtccatcgcc gaaatggcgg gccgccggct
     1621 catcggctgc tctatggagc tcggcgggaa gaatgccgcc atcgtccttc ccgatgcaga
     1681 tctgacgcgg acggtacgag agctggcttc ggccagcttt gccaacgccg gccagacctg
     1741 tgtcgccatg gagcggatct acgcccacga gtcgatccgc gaggagctca cccgaagact
     1801 cgccgaacac actgccgctt tgccggtgag cgccggcttc gacttctcga gcgagatcag
     1861 ctctatggtc gaccagggtc acctggaccg ggtccacgcc cacgtcgagg atgccgtggc
     1921 caagggagca acattgctca cagggggcaa gccccgtccc gacgtagggc cgctgttcta
     1981 tgcccccact gtcctcactg atgtcaccga ggagatggag ttgtgccggg aggagacatt
     2041 cggccccgtc accgcgatct acgggtacac ggacatcgcc gaggccgtcg cgcgagccaa
     2101 cgactcggaa ttcgggctcc acttcagcct gtggactcgc gacccggacc gcgcacgcga
     2161 tatcgctatc cggttggagg cgggatcggt caccatcaac gacgggctgg tggccagctg
     2221 gggctcacac gaggcgccga tgggtggcat gaagtcctcc ggtttgggtc ggcggcatgg
     2281 cctcgaggga atcctcaagt tcaccgagcc gcagacagtc gccacccagc gcctgatccc
     2341 tgcctattca ccattcggcg gcatcacccc ggagcgttac acccgattgg tcgagttcct
     2401 caccacggtg ttcaagcggc tccccttcta caagtagctg acatttccga ggatcggtac
     2461 agcaatcgaa gcagtcatct ctccgacatc tcggtcaggt cggcctccag gacgtcccgg
     2521 tcctccgggt cctcgatctc gagcagcttc tccttggccg cctccagatg ctcggtgaac
     2581 atgtcgatgt cgtcgacgtc ggcggcggca cgggcgatcg cctcatgggc gaatccggtg
     2641 acgaatgggt cgagatcgtt gtcgaggcaa acggtgagcg atcgctcgcc gaagcggagg
     2701 gcgagatcgg cgtagccgag gacggcggca acccgcgaga tctgccaatc ggagatcgcc
     2761 tgatttcgcg gcgtgccgac ctgataccag tgatagcgct gagcgaaggt cgcggccaac
     2821 atctcctctt cctgttcccg ggtcggctcg gtgccatcga ggagggccca tgcctcgttg
     2881 aaggccagag gggcgaaggt gtgatgccac gcctccagct cggccgggtc gggcttttcg
     2941 acaggatccg tcatcgttcg atctccgctg gattggggaa ccatcgcaga cgttcacccg
     3001 cgagacccag atcgggagaa acccctcctg gttcgggctc ctcggcgagg atccgcttcg
     3061 cttccgccag gtatgccccc aattcgctca gtggcgcggt catcggggct tcgatgccac
     3121 cgccggcgaa gtcgagatcc ctgagcgagg ctggcggctt gggcaggccg tcccgatgcc
     3181 gccacaacgc ggtctcgggt tcgtaggtgt acatggggag cagacgccaa ccctggtcgg
     3241 ccacgaggtg cactgcttcg atgaggtagg cgaattcgtc ctcggcgatg aaatagttga
     3301 agttgacccg gacccacccc ggcttgatga cttcgcaccc cgcggaaact gcatcttcga
     3361 tccgggaact ggtgtcgaga tcgatgccga gcaggcggtg cccgtagggc ccggcgcaag
     3421 aacacccacc ccgggcctga atgccgaaga ggtcgttgag gagggcaacc acgaagttgt
     3481 gatgaagcgt ccgctcgcca tgccggatca cgaaactgac tatcgagaga cgttcggcat
     3541 tcgggttacc caggatctcg atgttcgggt tgagtgacca ggactcgatg gctcgcctga
     3601 tgaactcgtg ctccaacgca cgaatggtgt cgacaccgac ggtctccttc aggcgaaaag
     3661 cgagaccggc ccggatcgac tcgatgatgg cgggcgtacc cccctcctcg cggtgctcgg
     3721 ggtcgctcag gtagatgtgg tggtcggggg ccacatactg gacggtgcct ccgccgggga
     3781 ccaccggcac ctcattgcgg agcaggtcgc ggcggacaac gagcaccccc ggcgtgccgg
     3841 gtccaccaat gagcttgtgc ggggagagga agatggcgtc cttgtattcg ccattgccat
     3901 cggcagagga gcccatctcg atgtcgacat acggcgcgga cgccgcgaaa tcccagaaag
     3961 tgagcgcgcc gtgggcatgg agcaggcgcg atatggccgc ggtgtcggag atgattcccg
     4021 tcacattgga ggcggctgag aaggagccga tgatcagcgg gcgatgggag tgctcgacga
     4081 gtctcgtctc cagttcgacg aggtctacat gccca
//
LOCUS       DASTXO010000456         4094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1279659, whole genome shotgun sequence.
ACCESSION   DASTXO010000456 DASTXO010000000
VERSION     DASTXO010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279659"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VFU96_09595"
     CDS             complement(<1..578)
                     /locus_tag="VFU96_09595"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEU5113561.1"
                     /translation="MLNAIIWGLVQGLTEFLPVSSSGHLILIPAFLNELGMDVQPPSL
                     AVGAVLHLGTLAAVLIYYRAEVMSVLRFRTNPDGRKIALLVGIGTIPALIGLPLADEV
                     ESLQESVAAVGWQIVATGVILVIGQRLAIGARNLLQGRIPDAIVVGIAQAIALIPGIS
                     RSGVTIAAGNWRKFEPAEAARFSFLLGIPAIAG"
     gene            complement(595..1113)
                     /locus_tag="VFU96_09600"
     CDS             complement(595..1113)
                     /locus_tag="VFU96_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113562.1"
                     /translation="MIWLQSPPWARWAVALLIASGALWIEFRPEPMTPHPFAADDVTA
                     GSIVDESNTEVRQVPLGTFTEVELGQVALDTILAGDPVLASDVGETGQTVPPGWWSLE
                     MPLPRGAHRGDSARIVLLDTEVVASGVVVEDPDEDPLGSGLGLVAVEPGLAANVARAA
                     GEGRAVVMVAPP"
     gene            1166..1978
                     /locus_tag="VFU96_09605"
     CDS             1166..1978
                     /locus_tag="VFU96_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113563.1"
                     /translation="MTDTTRTTLLVSGIVALVGSIIGLVLGLFMVNNLSDYLTQSVAV
                     SESALGAVEETLGVLDSVSVEVDEGIGAAAASIAAASEAVLTASGELDDVADFLDGEL
                     QSSIEALLDTMPAAIQTAGVIDRTLSALSFVGVDYDPEQPFDASLMAAADALETIPPQ
                     LTAQAETIRALVPVSEQFGEDAAAMAQAFDALRIELESSQTLIDSYRSTVDQAQSVVE
                     STASSLSTTTWLLRIIVVLMTVTGAAIAIGMITLGRSREPSMPTGFDEAAVG"
     gene            2088..2234
                     /locus_tag="VFU96_09610"
     CDS             2088..2234
                     /locus_tag="VFU96_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113564.1"
                     /translation="MTMGDKKKKKKKVEETAIAETHWVRSTVILLLVVAVTFGVWYFV
                     NERD"
     gene            complement(2244..2549)
                     /locus_tag="VFU96_09615"
     CDS             complement(2244..2549)
                     /locus_tag="VFU96_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEU5113565.1"
                     /translation="MPTYEYTCKRCGENIEVFQSFSEKPLRKHEACDGELQKVFHARG
                     VVFKGSGFYSTDSRSSASSKKSEPAAKKGESSKKSESATSGSTTASSSSSSSSATPD"
     gene            2640..3245
                     /gene="plsY"
                     /locus_tag="VFU96_09620"
     CDS             2640..3245
                     /gene="plsY"
                     /locus_tag="VFU96_09620"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU5113566.1"
                     /translation="MSPAVALPLAIIAAYLVGSVDFAVIVGRMYGVDIHQEGSGNPGM
                     SNVLRTMGRVPAAMVFIGDTLKGTIGAAMGWVASGSPDPAVHWAFLAGLAAVVGHCYP
                     IFHGFKGGKGVATGGGVLLFTVPIVAVIEIAIWFLVFWITKTASIGSMVIVVITIPML
                     IWQGVSGLSLVWVALIIALVVWRHRGNIKRMVSGSEEKVST"
     gene            3242..4054
                     /locus_tag="VFU96_09625"
     CDS             3242..4054
                     /locus_tag="VFU96_09625"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEU5113567.1"
                     /translation="MSIEVAVIPAAGRGTRMRPATRVVPKALLTVIDRPAIQYSVEEA
                     ARAGAKEAVVVVDLDAGHLLAQHFNIEGPLPGLEDIQLRPVVQEEPLGLGHAVNEAAH
                     MVGDRPFFCLLSDNIVRPGHDVLASLGDGSRDGTTSVMCLRELTEEFLSKYGVVVPAS
                     DLEDRYLDLAGAVEKPGPVNAPSRLGLVGRYLFTPEVFEILGSLEPGHGGEIQLTDAI
                     NELGARGRLRGYVAEEDLLDVGTPIGLLEASVELGEHEFGQEFSSWLKERSG"
BASE COUNT          715 a         1281 c         1359 g          739 t
ORIGIN      
        1 ccggcgatgg caggaatgcc gaggaggaat gaaaagcgtg cggcctcggc cggctcgaac
       61 tttcgccagt tgccggcagc gatggtcaca cccgaccgcg atatccccgg gatgagtgcg
      121 atggcctggg cgatgccgac gacgattgcg tccggtatcc ggccttggag cagattccgg
      181 gccccaatgg cgagacgctg gccgatcacg agaatgacac cggttgccac gatctgccac
      241 cccaccgcgg ctaccgactc ctggaggctc tccacctcgt cggccagagg cagcccgatg
      301 agcgcgggta tggtgccgat cccgaccagt agggcgatct tgcgaccgtc cggattggta
      361 cgaaagcgga ggacactcat cacctcggcc cggtagtaga tgaggaccgc ggcgagcgtg
      421 ccgagatgaa gcacggcgcc tacggccagc gacggcggct ggacgtccat gcccagctca
      481 ttgagaaacg ccgggatgag gatcaggtga cccgacgagg acaccgggag gaactcggtc
      541 agtccctgga ccagccccca gatgatggcg ttgagcacgg cggcacggta atgctcaggg
      601 cggggcaacc atgaccaccg ctcgcccctc cccggccgcc ctggcgacgt tggcagcgag
      661 gccgggctcg acggcgacca gacccaaccc ggagccgagc gggtcttcgt ctgggtcctc
      721 gaccaccacc ccggaagcta ccacctcggt gtcgagcaag acgatgcggg ccgagtctcc
      781 ccgatgcgcc ccacgtggca agggcatctc cagactccac caccccggag gcaccgtctg
      841 tcccgtctcg cccacgtccg acgccagcac cggatcgccg gcgaggatgg tgtcgagggc
      901 cacctgcccc agctcgacct cggtgaaggt cccgagaggg acctgtcgga cctcggtgtt
      961 ggactcgtcg acgatcgatc cggccgtcac atcgtcggcg gcgaaggggt ggggtgtcat
     1021 tggctcgggg cggaactcga tccacaaagc cccggaggcg atgagcaggg cgacagccca
     1081 acgtgcccac ggcggagact gcagccagat cacctgttcg aaagtacgta atgcggacgg
     1141 gcctgagaag taggttggct ccccaatgac ggacacgacg agaacgacgc tgctcgtgag
     1201 tggaatcgtg gccctggtcg gcagcatcat cggcctggtc ctcggcctgt tcatggtgaa
     1261 caacttgtcc gactatctca cccagtcggt agcggtctcg gagtctgcat tgggagcggt
     1321 ggaggagact ctcggcgtcc tcgattcggt ctcggtcgag gtcgacgagg gcatcggagc
     1381 ggccgccgcc agtatcgccg cagcctcgga ggctgtcctc accgcatcgg gggagctgga
     1441 tgacgttgcc gacttcctcg atggtgagtt gcaatcgagc atcgaggccc tcctcgacac
     1501 catgccggcc gcgatacaga ccgccggggt catcgatcgc actctgagcg ctctgtcctt
     1561 cgtcggcgtg gactacgacc cagagcagcc tttcgacgcg tccctgatgg cagctgcaga
     1621 tgccttggag acgatccctc ctcagctcac cgcccaggcg gagaccatca gggccctggt
     1681 tccggtatca gagcagttcg gcgaggacgc cgccgccatg gcgcaggcct tcgacgcctt
     1741 gaggatcgag ctggagtcga gtcagacact gatcgattcc taccggtcca ccgtcgacca
     1801 ggctcagagc gtggtcgaga gcaccgcttc gtccctgtcc accacgacat ggctgctgag
     1861 gatcatcgtc gtgctcatga cggtgaccgg agcggccatc gccatcggga tgatcaccct
     1921 cggccggtct cgagagcctt cgatgccgac cggtttcgac gaagctgccg tcgggtaggt
     1981 ggccgaggag aggtgtgtgg acccgcgatt cgtgaatcat cggaggccac tgatcttcca
     2041 cgggttgggg tccacctcct cctgaatgcc gccggaacca ggagatgatg acaatgggcg
     2101 acaagaagaa gaaaaagaag aaggttgaag agaccgcgat cgcggagacc cactgggttc
     2161 gatcgactgt gatcttgctg ttggtcgtgg ccgtgacgtt tggcgtctgg tacttcgtca
     2221 acgagcggga ctaacgggga ctgtcagtcg ggcgtagcag agctgctcga ggagctcgag
     2281 gaagcagttg tcgacccgct tgtggctgac tcgctcttct tcgaggattc gcccttcttg
     2341 gcggcgggct cactcttctt ggacgatgcc gacgagcggg agtcggtcga atagaagcca
     2401 gatcccttga acaccacgcc ccgggcgtgg aacaccttct gaagctcccc gtcgcaagcc
     2461 tcatgcttgc gcagcggctt ctcagagaac gattgaaaga cctcgatgtt ctcgccgcag
     2521 cgcttacagg tgtattcgta ggtaggcatg accgacttct gaccgcttgg tggggtaggg
     2581 gcgaattcta caaccgggac aatgggtctt ccattcctct tgcaatatga tcggcaccca
     2641 tgtcgcccgc tgttgccctg ccgctggcga tcatcgccgc ctatttggtg gggagtgtcg
     2701 acttcgcggt gatcgtcggc cggatgtacg gcgtcgacat ccatcaagag gggagcggca
     2761 atccaggcat gtcgaacgtg ctccgcacga tgggacgggt gcccgccgcc atggtgttca
     2821 tcggagacac cttgaaggga acgattgggg cggcgatggg ttgggtcgct tctggctcac
     2881 ccgaccccgc ggtgcactgg gcgttcctcg ccgggctggc cgcagtcgtc ggccattgct
     2941 atccgatctt ccacgggttc aaagggggta agggcgtagc caccggcggt ggtgtccttc
     3001 tcttcaccgt ccccatcgtg gccgtcatcg aaatcgccat ttggttcctg gtcttctgga
     3061 tcaccaagac tgcctccatc ggctcgatgg tgatcgtcgt catcaccatc ccgatgctga
     3121 tctggcaggg cgtctcgggg ttgtccctgg tctgggtggc cctgatcatc gcgttggtgg
     3181 tgtggcggca ccgtggaaac atcaaacgga tggtctctgg cagcgaggag aaagtctcga
     3241 catgagtatc gaagtggccg tcatccccgc cgccggccgt gggaccagga tgagaccggc
     3301 gacccgcgtg gtgcccaagg ccttgctcac ggtgatcgat cgcccggcaa ttcagtactc
     3361 ggtcgaggaa gcggcgcggg cgggggccaa ggaggcggtc gtcgtcgtcg atctcgatgc
     3421 cggtcatctg ctcgcccagc atttcaacat cgagggccca ctccccggtc tcgaggacat
     3481 ccagttgcgc cctgtggtcc aagaagagcc gctcggcttg gggcatgcgg tcaacgaggc
     3541 ggcgcacatg gtgggcgacc ggcccttctt ctgcctcctc tccgacaaca tcgtgcgacc
     3601 cggtcacgac gtcctcgcca gcctcggcga cggaagtcgc gacggcacca cgtcggtgat
     3661 gtgtttgcgc gagctgaccg aggagttcct cagcaagtac ggcgtggtgg tgccggcctc
     3721 cgacctggag gatcgctatc tcgacctcgc cggcgccgtc gagaagccag gcccggtcaa
     3781 cgcgccctcg cggctcgggc tggtcggccg ctatctcttc actccggagg tcttcgagat
     3841 cctgggctcg ctggagcccg gccatggtgg cgagatccag ctcaccgacg ccatcaatga
     3901 actgggtgcc cgcgggcgac tccgcggata cgtcgccgag gaggacctgc tcgacgtggg
     3961 cacgcccatc gggttgctcg aggcttcggt cgagctgggt gagcacgagt tcgggcagga
     4021 gttcagctcc tggctcaagg agcgaagcgg gtgaaaccgc tggaggaggc gcgctccgag
     4081 gtgctctctt cggt
//
LOCUS       DASTXO010000457         3152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1648212, whole genome shotgun sequence.
ACCESSION   DASTXO010000457 DASTXO010000000
VERSION     DASTXO010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1648212"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VFU96_09630"
     CDS             <1..280
                     /locus_tag="VFU96_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU5113568.1"
                     /translation="AEREVEIILVRAPGATETLARKVVSAVGRLRQLDLAKPPGVAET
                     LDWVATLNVLGAEDLDPRTVEDTLGAVIKERDDLETVLDHVEELVADG"
     gene            273..1439
                     /locus_tag="VFU96_09635"
     CDS             273..1439
                     /locus_tag="VFU96_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU5113569.1"
                     /translation="MADDSVAVGEAGPADRTSFIGMLVGFGDELRAAGLPIGSGDLLT
                     FCAAMALLDPGDVLDLYWAGRTSLVARHDHIPIYDRVFRIYFLDDPGDLDEPARAVLK
                     QSQIENQSVIEVPSAEPTQPGKEEEARLGLMASDIEIWRNKAFSACTDEELAALRRIM
                     NQMKVIPPRRRSRRTTKSRAGSRPDLRRTVRTTMRTHGEPSELFWRQRRLKMRPLVLI
                     LDVSGSMAEYSRNLLQFAYSTRRAATRVEVFCFGTRLTRITRALERRRPDDALDQAAR
                     SVFDWEGGTRIGHAIDTFVRQWGRRGMSRGAIVVICSDGLDRGDPAVLAAAMERLSRL
                     CHKVVWMNPHKGDNPEFIPTTLGMMVAAPHIDLILSGHDLASLEELAERLPELR"
     gene            1444..1737
                     /locus_tag="VFU96_09640"
     CDS             1444..1737
                     /locus_tag="VFU96_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113570.1"
                     /translation="MRWSVSLQAEGDRVIELEEVVELADAVAASNGIASGMGTFGYGV
                     QVVVEADNGDRAVELAMVVLTEAVEKSGLPSWPVVSVETIAEDEDIFYDMVEE"
     gene            1734..2183
                     /locus_tag="VFU96_09645"
     CDS             1734..2183
                     /locus_tag="VFU96_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113571.1"
                     /translation="MIRLGSLAGYPFEGPRVLGGWTAPERSAVFAVMYKPDPARKPEE
                     YGVIYVGHSDDLSSERFPFDHPRAGCWLERVGGKKFDLYIGIYEAPGANARHREQIVR
                     ELIAIYHPGCNGDQYVQAWEEHWIGEYETPGTTGPLTTRRDPSAAGG"
     gene            complement(2193..3059)
                     /locus_tag="VFU96_09650"
     CDS             complement(2193..3059)
                     /locus_tag="VFU96_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113572.1"
                     /translation="MTHDQIDRMVRKANPLPDPTALELVDVPVLMTPLERRTEMQIDD
                     RPGNEVQADRNRRRGPIVGIAAAAVILIAGLVLFLTRDNMPVAEPAPNATEITPEEAL
                     EAESPLEPGAYFVDTDGDDASSLRGTFVIEGSGWGPVPSGAVKGSEGQEFVMLLVLEV
                     DQVYSPVCDVPAPSPVAAGTTAEDLANQFAASGFTVREALAPVNAFGYEGHHMVVEVP
                     EGCAGETYQAWTGGGWSGRYYQSNGNVVEYWFLDVEGTPVMVEADWFPGTSAEEDVAE
                     LQAVVDSLVITP"
BASE COUNT          552 a         1027 c         1026 g          547 t
ORIGIN      
        1 cgccgagcgc gaggtggaga tcatcctggt tcgggccccg ggggccactg agaccctggc
       61 gcgcaaggtc gtgtcggcgg tcggccgcct ccgccagctg gatctggcca agccgcccgg
      121 cgtcgccgag accctcgact gggttgcaac gctgaacgtg ctgggcgccg aggacctcga
      181 ccccaggacg gtggaggaca ccctcggcgc cgtcatcaag gagcgcgacg acctggagac
      241 cgtcctcgac cacgtcgagg agctcgtcgc cgatggctga cgactcggtc gccgtcggag
      301 aggcgggtcc tgccgaccgg acgtctttca tcgggatgct ggtcggcttc ggcgacgagc
      361 ttcgtgcagc cgggctcccg atcggatcgg gtgatctgct cacgttttgc gcggcgatgg
      421 cgttgctcga cccgggcgac gtgctcgacc tctactgggc cggacgaacg tcgctggtgg
      481 cgcggcatga tcacatcccc atctatgacc gggtcttccg catctacttc ctcgacgatc
      541 ccggcgatct cgatgagccc gcacgagcgg tcctgaagca gtcgcagatc gagaaccagt
      601 cggtgatcga ggttccctcc gccgagccga cacagcccgg caaggaagag gaggcccggt
      661 tgggactgat ggcctccgac atcgagatct ggcggaacaa ggcgttttcg gcctgtacag
      721 acgaagagct cgccgccctg cgccgcatca tgaaccagat gaaggtcatc cctccccgtc
      781 gccgttcgcg gcgcacgacc aagtctcggg ccgggagcag acccgacctc cgtcgcacag
      841 tccggaccac gatgcgcact catggagagc catccgagtt gttctggagg cagcgtcggc
      901 tcaagatgcg cccgctggtc ctcatcctcg acgtgtcggg ctccatggcc gagtactcgc
      961 gcaatctcct ccagttcgcc tactcgacac ggcgagcggc gaccagggtg gaagtcttct
     1021 gcttcggcac gcggctcaca cggatcacac gggccctgga gcgccgacgc cctgacgacg
     1081 ccctggacca ggcggccagg tcggtcttcg attgggaggg tggcacccgc atcgggcatg
     1141 ccatcgacac cttcgtccgc cagtggggtc ggagggggat gagtcgggga gcgatcgtcg
     1201 tcatctgctc ggacgggctc gaccgtggtg acccggcagt cctggccgca gccatggagc
     1261 ggctatcccg gctctgtcac aaggtggtgt ggatgaatcc ccacaagggc gataacccgg
     1321 agttcatccc gacgaccctc ggaatgatgg tggccgctcc tcacatcgac ctgatccttt
     1381 ccggccacga tctggccagc ctcgaagagt tggccgagcg gctgccggag ttgaggtgag
     1441 ccattgagat ggagtgtctc cctccaggcc gaaggtgacc gagtgatcga gctcgaagaa
     1501 gtggtcgagc tcgcggacgc ggtggccgcc agcaacggga tcgcctcggg gatgggaacg
     1561 tttggatacg gggttcaggt cgtcgtggag gcagacaacg gcgatcgtgc cgtggagctg
     1621 gcgatggtgg tgctcaccga ggccgtggag aagtccggtc tcccgtcctg gccggtggtc
     1681 agcgtggaga ccatcgccga ggacgaagac atcttctacg acatggtcga ggagtgatcc
     1741 ggctcggctc cctcgccggg tatccctttg aagggccgcg cgtgctcggg ggttggaccg
     1801 cacccgagcg atctgcggtt ttcgccgtca tgtacaagcc ggacccggcc cgcaagcccg
     1861 aagagtatgg agtcatctac gtcgggcact ccgacgacct atcgagtgaa cgcttcccct
     1921 tcgatcaccc cagggctgga tgctggctgg agcgggtggg cgggaagaag ttcgacctct
     1981 acatcggcat ctacgaggca cccggtgcca acgcccggca ccgcgagcaa atcgtccgag
     2041 agctgatcgc catctatcac cccgggtgta acggggatca atacgtccag gcgtgggagg
     2101 agcactggat cggggagtac gagaccccgg gcaccaccgg accgctgacg actcgaaggg
     2161 acccgtcagc ggccggtggt tgaagatacc gactagggag tgatcaccag gctatcgacc
     2221 acggcctgaa gttcggccac atcctcctca gcggaagttc cggggaacca gtccgcttcg
     2281 accatgaccg gcgtgccctc cacgtctagg aaccagtatt ccaccacgtt gccgttgctc
     2341 tggtagtaac gcccactcca tccgccgcct gtccaggcct ggtacgtttc gccggcgcac
     2401 ccttcaggca cctcgacgac catatggtgc ccttcgtagc cgaaggcatt gaccggtgcc
     2461 aacgcctccc ggaccgtgaa tccactggcc gcgaactggt tggccagatc ctccgccgtc
     2521 gtcccggcgg ctaccggaga gggagcgggc acatcacaca ccggtgaata cacctgatcg
     2581 acctcgagga caagcagcat cacaaactcc tgaccctcac ttccctttac cgcccccgat
     2641 ggcactggcc cccacccgct gccttcgatg acgaaagtgc ctcgaaggga cgaagcatcg
     2701 tctccgtcgg tgtcgacgaa gtaagcgccg ggctcaaggg gcgactccgc ctcaagtgcc
     2761 tcctcagggg tgatctcggt cgcattcggg gccggctcgg ccactggcat gttgtccctg
     2821 gtcaggaaca agacgagccc tgcgatcagg atcaccgccg ccgccgcgat ccccacgatg
     2881 ggaccccgcc ggcgattccg gtccgcctgg acctcattcc cgggtctgtc atcgatctgc
     2941 atttccgtcc tcctctccag aggggtcatg aggacaggca catcgacaag ctcgagggca
     3001 gtcggatccg gcagcggatt tgctttgcgg accatgcgat cgatctgatc gtgtgtcatg
     3061 tcgctccttc ctcggcgatc ggaggggaag ggatgggctt gatgagaggc tcgagcgccc
     3121 gagcgagtct ctgataggaa cggtggattc tc
//
LOCUS       DASTXO010000458         2219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1795678, whole genome shotgun sequence.
ACCESSION   DASTXO010000458 DASTXO010000000
VERSION     DASTXO010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1795678"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1411)
                     /locus_tag="VFU96_09655"
     CDS             complement(<1..1411)
                     /locus_tag="VFU96_09655"
                     /inference="COORDINATES: protein motif:HMM:NF023650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEU5113573.1"
                     /translation="MKKHLSAVKTPLGALVVGVAGLLLLLFTAYGLTRLASRGEVMGR
                     VEVAGTQIGGLDEEQALSALLAVEEEYLGRPAVFNIEGKFVSMQPPEAGLDINEQAIA
                     DEALLVGRNGNIVSEFVWWLTHIFDTVQVPVRGSVDDVAIEEVFDVWDAEVIALPPSL
                     GGVVLDDGRPSPVYPEVGTGVDRPPARAIVEATLLALEPEQTSIPTATVVPVLTDEDV
                     DRAVAEAEAMLVEPIQLDHEDVEVVFTVDQLTEAFRSETVTESEPRIVNSFDPEAIDG
                     YLSPIRNEVEAEPVNATYEISGDSISIVPGSSGTRIDADETAVRLADAAKTAGRSGEL
                     PLVEAAEPEVTTEYLEGLNINHLVAQFTTYHDCCEDRVVNIHRIADEVDGAIVLSGQT
                     FSLNDHVGQRTLEDGYLPAGTIIAGEVEDTVGGGVSQFTTTLYNAVFWGGYEDVEHKP
                     HSYYFSRYPEGVEATLNWRS"
     gene            1517..>2219
                     /locus_tag="VFU96_09660"
     CDS             1517..>2219
                     /locus_tag="VFU96_09660"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEU5113574.1"
                     /translation="MTIRFPEMVNGDAIRVSASTYVTFKKCPAQAGARLQGIYGQESR
                     QSFLGILAHRVFSRHLTSGAIPSEEFVQACREEIGGSALNNKMAGLEMRPSALAGIIE
                     EVRGLYERFTRLPGEGFEGSEHSIEATVADGVDLVGTVDAVYREDLGGFRLVDWKTGE
                     LGDTEDQLLFYALLWLLDRGELPAYVEAVSVRTGERYRTVPSSADVREVAVEIGELVD
                     LIRRAWATGVDLPRTG"
BASE COUNT          371 a          702 c          730 g          416 t
ORIGIN      
        1 ggcttcgcca gttgagcgtc gcctccaccc cctctggata gcgtgaaaaa tagtacgagt
       61 ggggcttgtg ttcgacatcc tcgtagcctc cccagaacac cgcgttgtag agcgtcgttg
      121 tgaactggct caccccgcct ccgacggtgt cctcgacttc gccggcgatg atcgtcccgg
      181 ctggtagata cccgtcctcg agggtgcgtt ggcccacgtg gtcgttgagg ctgaaggtct
      241 gtccggagag gacgatcgcc ccgtccactt cgtctgcgat ccggtggatg ttgaccacgc
      301 ggtcctcaca gcagtcgtga taggtggtga actgcgccac caggtgattg atgttgaggc
      361 cctcgaggta ttcggtcgtg acctcgggct ccgcggcttc gacgagcgga agctccccgc
      421 tgcgacccgc agtcttcgcg gcatcggcca gacgcaccgc ggtctcgtcc gcatcgatcc
      481 gggtgccgct cgagccgggg acgatcgaga tgctgtcacc cgagatctcg taggtcgcgt
      541 tcaccggctc ggcctcgacc tcgtttcgta tggggctcag gtatccatcg atcgcctcgg
      601 gatcgaagga gttgacgatg cgtggctcgc tctcggtcac cgtctcggat cggaaggcct
      661 cggtcagctg gtcgacggtg aacaccactt cgacgtcctc gtggtcgagt tggatcggtt
      721 cgacgagcat cgcctcggcc tccgcgacgg cgcgatcgac gtcctcgtcg gtgaggaccg
      781 gtacgacggt tgcggtgggg atggaggttt gctccggttc gagggcgagc aaggtggcct
      841 cgacgatggc cctggctgga gggcggtcga cgccggtccc gacctcggga tagaccgggc
      901 tcggtctgcc atcgtcgagg accacgcctc caagtgatgg gggcagtgcg atcacttccg
      961 cgtcccagac gtcgaacacc tcttcgatcg ccacgtcgtc caccgagccc ctgaccggta
     1021 cctgaaccgt gtcgaagatg tgggtgagcc accagacgaa ctcggagacg atgttgccgt
     1081 tgcgccccac cagcagcgcc tcatcggcga tcgcctgctc gttgatgtcc agtcctgcct
     1141 ccgggggctg catgctcacg aacttgcctt cgatgttgaa caccgccggg cggcccaggt
     1201 actcctcttc gacagccagc agcgcgctca gagcctgctc ttcgtcgaga ccgccgatct
     1261 gggtgcccgc cacctccacc cgccccatca cctcaccacg agaggcgagg cgggtcaggc
     1321 cgtacgccgt gaacaagagc agcagaaggc cggcaacacc cacgacgagc gcgccaagtg
     1381 gagtcttgac tgcggagaga tgctttttca tggaaggccg ccccaaaacg acctttttgc
     1441 ccggggccgg cgatggtaat gagcaagtcg cccttatccg aaaatgtgac agggctgctc
     1501 ggtaccctcg attcgagtga cgatccgatt cccggaaatg gtcaatgggg acgccatcag
     1561 ggtctcggcg tcgacctacg tgacgttcaa gaaatgcccg gcccaggccg gggcgaggct
     1621 gcaggggatc tatggccagg aaagccgtca gtccttcctc ggcatcctcg ctcaccgggt
     1681 cttcagcagg cacctgacct cgggcgccat cccatccgaa gagttcgtgc aggcctgccg
     1741 ggaagagatc ggcggctccg ccctgaacaa caagatggcc gggctcgaga tgcggccgtc
     1801 ggctctcgcc ggaatcatcg aagaggtgcg gggcctgtac gagcggttca cccggctccc
     1861 cggagagggt ttcgaagggt ccgagcacag tatcgaggcc actgtcgccg acggagtcga
     1921 tctggtcggc accgtcgatg ccgtctaccg cgaagacctt ggtgggttcc gcttggtcga
     1981 ctggaagacg ggcgagctcg gggacaccga ggatcagctg ctgttctatg cgcttctgtg
     2041 gctgctcgat cgcggggagc tacccgcata cgtcgaagcg gtctccgtta ggacgggaga
     2101 gcggtatcgc acggtgccgt cgtcggccga cgtgagggaa gtagccgtcg agatcggtga
     2161 gctggtcgat ctcattcgca gggcctgggc aaccggcgtc gatcttcccc ggacgggcg
//
LOCUS       DASTXO010000459         4510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_359311, whole genome shotgun sequence.
ACCESSION   DASTXO010000459 DASTXO010000000
VERSION     DASTXO010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359311"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1574)
                     /locus_tag="VFU96_09665"
     CDS             complement(<1..1574)
                     /locus_tag="VFU96_09665"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEU5113575.1"
                     /translation="MSRVVGVDVGGTFTDVVVLDGSIVGYKVRTTPDQSDGVVRGLSD
                     RVDDTTTILHGTTAGTNALIEGRGARTALVTTHGFEDLVEIGRQARPSLYDSFADRPV
                     ALSPRSLRFGYAGDVEAVGAVVEQAAPEAIAVALVRSYTDPSDEIDLAEKLRELVDIP
                     VSVGALVSPQFREYERIATTVLNAYLTPELAGYLSALDQRVGGGRRLVMTSSGGLLPF
                     SAASAYAGRLVLSGPAAGVVAATAVGRAKGFESIISFDMGGTSTDVCRVDDGAIQSSS
                     RIGSGRVNRVPSIPVRTIGAGGGSLAWVDEGGALRVGPTSAGAHPGPAAYGRGGVQAT
                     VTDANVVLGRIPADLSLGGSVHLDIGAARRALALLGAELGLEIDAVAAGIVEVFDTHM
                     ERALRAVSVEEGVDPRDSVLVAFGGAGGLHATRLARRLGMTRTLIPPLSGVFSALGLL
                     LASPSSDTSRTVMLEEGSARLARMAEEILDEAGRTYLSDHAGDFMQRRAWADVRYAGQ
                     SHELSVSLVPDWSRLRR"
     gene            1595..1852
                     /locus_tag="VFU96_09670"
     CDS             1595..1852
                     /locus_tag="VFU96_09670"
                     /inference="COORDINATES: protein motif:HMM:NF020295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HEU5113576.1"
                     /translation="MAVIVAPSPNPNAMKFTVGKPVGGPTTHIAGTPPEQSFARDLLA
                     IPGVTSIFMTADFVTISKTSDVTWESIIPEATSILEGEFGG"
     gene            complement(1853..2686)
                     /locus_tag="VFU96_09675"
     CDS             complement(1853..2686)
                     /locus_tag="VFU96_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113577.1"
                     /translation="MEPERHETYERIPWETLEEKKPDRQWLMMAVAGAVVLGALAYSF
                     MSSRPAPVPVTSSVAPVDPEITVPLPPPAQAMAPAVPSTAPIVTAEADLYAVHPERVI
                     DRAVAHAEWFVAEYLTVDGSDEGRAALTALTPTGVPLPTATEGARVFVEWVRASAVEE
                     IGQLLYRVTVLARSLAAAPNAEYQRQAPLQIEVDVSVAGEAPQVVMAPRVSPAPSGPA
                     HQLALAPVPDEVGAAALQQAGASEVVGGKQAPDGAWQVVVLAEMPDGVSRPVSVLVPP
                     P"
     gene            2789..3403
                     /locus_tag="VFU96_09680"
     CDS             2789..3403
                     /locus_tag="VFU96_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113578.1"
                     /translation="MAVNPDPKPGRWILPLVILGMIAFTYFFVRSLPEASPDTTLAAG
                     DTTPTTVGGDGPTTTSTPGGTGSIPDAAAAYISELDRINGEFQALNNELVAVNTGFDA
                     DPREIEFGDAETRIEAVATSAQALSDQVAALTPPAGLEANQEALAAAASLAARAAGEA
                     LSGLQSADPGDLRRAAVTAFTGAVNDFDVEVQNARTAAGAAPDA"
     gene            3403..>4510
                     /locus_tag="VFU96_09685"
     CDS             3403..>4510
                     /locus_tag="VFU96_09685"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF002367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5113579.1"
                     /translation="MAVEIRAIGPDEFEAMRRTMGLVFGFDPPEGEGRFLRVLPLDRT
                     RCGFDGGTMISTSGAFSLEMTVPGGQVACGGTTAVAVVPTHRRQGVLREMMRAHLADV
                     KEHGEPIAALWASDSAIYGRFGYGCASICYDFEVEPQHAAANRLAPEPGQSRLIDRKE
                     ALEIVPPLYDRLRMGIPGFFARTPEWWEDRSFRESGTSGGGSTALRYGVVDGDDGISG
                     FASYRINARWDEGHGAGKVTVHDLFAETPEAWAGIWSLVTNQDLVARAVGGLRPPWDP
                     IFDLLAGTRRARAYRYDALWVRLMDVAAALAARSYSAPLDLVLGVEDPLGDATGSYRL
                     HADSDGAACAPTTDEPTVLLDLEDLSAGYMGRPRF"
BASE COUNT          777 a         1539 c         1473 g          721 t
ORIGIN      
        1 cggcgaagcc gcgaccagtc ggggacgagg ctcaccgaga gctcatgtga ctgaccggcg
       61 tacctcacgt ccgcccaggc tcgccgttgc atgaaatcgc ccgcgtggtc ggagaggtat
      121 gtcctccctg cctcatcgag gatctcctcg gccatccttg ccagccgggc cgagccctcc
      181 tcgagcatga ccgttcgact cgtatcgctg ctggggctgg ccagcaacag accaagggcc
      241 gagaacacgc cagataacgg ggggatcagg gtcctcgtca tgccgagacg tcgtgcgagg
      301 cgggttgcat gcagcccacc ggcgcctccg aatgcaacca ggaccgaatc ccggggatcc
      361 actccctcct ccactgacac cgcacgcagg gctctctcca tgtgggtgtc gaacacctcg
      421 acgatgccgg ccgccaccgc atcgatctcg aggccaagct cggcaccgag cagtgcgagg
      481 gcgcgccgcg ccgccccgat gtcgaggtgc actgatccac cgaggctgag gtcggctgga
      541 atgcggccga gtaccacatt cgcgtctgtt acggttgcct ggacaccacc gcgcccatat
      601 gcggccgggc cgggatgagc acccgccgac gtcggcccta cccgaagcgc gcctccctca
      661 tcgacccacg ccagactccc accgccggcc ccgatggttc ggaccgggat cgagggcacc
      721 cgattgactc tgcccgaccc gatacggctc gacgactgga tcgcaccgtc gtcgacccgg
      781 cagacatcgg tcgatgtgcc gcccatgtcg aagctgatga tcgattcgaa tcctttggcc
      841 cggcccacgg cggtggcggc caccaccccg gcggccggac cggagaggac gagccggccg
      901 gcgtaggcgg atgccgcgga aaagggaagc aggccgcctg acgaggtcat gaccagacgc
      961 cgcccgcctc caaccctctg atccagagcg gacaggtatc cggccaactc cggggtcaga
     1021 tatgcattga ggactgtggt ggcgattcgc tcgtactccc ggaactgagg cgacaccaga
     1081 gcgccaaccg acaccgggat gtcgaccaac tcccgcaact tctccgcgag atcgatctcg
     1141 tctgatggat cggtgtatga ccgcaccagg gcgacggcga tggcctcggg agccgcttgc
     1201 tcgacgacgg cccccacggc ctcgacgtcg cccgcgtatc cgaatcgcag gcttcggggg
     1261 gagagtgcca ccggtctgtc tgcgaaggag tcgtacagcg aagggcgagc ctgacggccg
     1321 atctcgacaa ggtcctcgaa cccatgggtc gtgaccaatg cagtcctggc gccccttcct
     1381 tcgatcagag cattggtgcc ggctgtcgtc ccgtgaagaa tcgtcgtggt gtcgtcgacc
     1441 cggtccgaga gaccccggac cacaccgtcg gactggtcgg gtgtcgttcg caccttgtac
     1501 ccgacgatcg agccatcaag cacgaccaca tcggtgaaag tgcccccaac gtccaccccg
     1561 acgacacggg acacccgatt agcctccgcc ggaaatggca gtgatcgtcg ccccctcacc
     1621 aaacccgaat gccatgaagt tcacggtggg caagcctgtt ggcgggccga cgacacatat
     1681 tgccgggaca ccgccggagc agtcgttcgc tcgtgatctc ctcgccatcc cgggcgtgac
     1741 cagcattttc atgaccgccg acttcgtgac catctccaag acttcagacg tcacctggga
     1801 atcgatcatc cccgaggcca cctcgatcct agagggggag ttcgggggct gatcaaggag
     1861 ggggaaccag caccgacacc ggccggctga ccccatcagg catttcggcg agcaccacca
     1921 cctgccaggc accgtcggga gcctgtttgc ccccaacgac ctccgatgcc cctgcctgct
     1981 ggagcgcggc ggctccgacc tcatcgggca ccggtgcaag cgcaagttga tgggcgggtc
     2041 cggacggggc aggggagacc cgtggcgcca tcacgacctg aggggcttcg ccggcgacgg
     2101 acacgtccac ctcgatttgc agtggcgcct gacgctgata ctccgcattc ggggcggcgg
     2161 ccaacgatcg ggcgaggacc gtcacccggt agaggagttg gccgatctct tccacggccg
     2221 aggcacggac ccattccacg aacacccgag cgccctccgt cgccgtcgga agcggcacac
     2281 cggtcggcgt caatgccgtc agggcggccc tgccctcgtc tgagccgtcg acggtcagat
     2341 attcggccac gaaccattcg gcgtgcgcca cggcacgatc gatgaccctt tccgggtgga
     2401 cggcatagag atcggcttct gcggtcacga tcggagccgt cgaaggcacc gccggggcca
     2461 tggcctgagc cggaggaggg agcgggacag tgatctccgg gtcgacgggg gccaccgacg
     2521 acgtgactgg aaccggggcc ggccgactgc tcatgaacga ataggcgagg gcaccgagca
     2581 cgacggctcc ggccacagcc atcatcagcc actgacggtc gggcttcttc tcctccagcg
     2641 tttcccaggg gatccgctcg taggtctcat gtctctccgg ctccatctca cgaacgtatg
     2701 tgctggacgt agccggtgca aggggtctga gagaccccgg agatgaggcg cagggaacta
     2761 ccaggtgcca gctatcgttt cgccggtcat ggctgtcaac cccgacccga aaccgggccg
     2821 ctggatcctc cccctggtca ttctgggaat gatcgcgttc acctatttct tcgttcgctc
     2881 actgcccgag gcatcgccag acacgacgct ggcggccggt gacaccacac cgacgactgt
     2941 cggtggcgac ggccccacca ccacgagcac gccgggcggc acgggctcga tccccgatgc
     3001 cgctgcggca tacatctccg agttggaccg gatcaacggc gagttccagg cgctcaacaa
     3061 tgagttggtc gcggtcaaca ccggattcga cgccgaccca cgcgagatcg agttcggtga
     3121 cgcggagacg agaatcgagg cggtggcgac ctcggcgcag gcgctctccg atcaggtggc
     3181 ggcactcact cccccggccg gtctcgaagc caatcaggaa gcgttggcag ccgcggccag
     3241 cctggccgcc cgggccgccg gcgaggcgct ctccggactc cagtcggcag accccggtga
     3301 tctgcgccgg gccgcggtga ccgcgttcac tggcgccgtg aacgacttcg acgtcgaagt
     3361 gcagaatgct cgcaccgcag cgggggccgc acccgacgcc tgatggccgt cgagatccgg
     3421 gccatcgggc ccgacgagtt cgaggcgatg cgacggacca tgggcctggt gttcggcttc
     3481 gatcctccgg aaggagaagg tcggttcctg cgtgtcctgc ccctcgaccg aactcgctgc
     3541 ggattcgatg gcgggacgat gatcagcaca tccggcgcgt tcagcctcga gatgaccgtg
     3601 cccggtgggc aagtcgcctg cggcgggacc accgcggtgg cagtggtgcc cacccatcgg
     3661 cgccagggcg tgcttcggga gatgatgcgt gcccacctcg ccgatgtgaa ggagcatggc
     3721 gagcccatag ccgcgttgtg ggcttctgac agcgccatct atggccggtt cgggtatgga
     3781 tgcgcctcga tctgttacga cttcgaagtc gagccccagc acgcggcggc gaatcgtctc
     3841 gcgcctgagc ccgggcagtc ccggctgatc gaccgcaagg aggcactgga gatcgttcca
     3901 ccgttgtacg accggctgcg catggggatc cctgggttct tcgccaggac ccccgaatgg
     3961 tgggaggacc gctcgttccg ggagagtgga acctccggag gtggcagcac cgccctccgc
     4021 tatggggtcg tggatgggga tgacggcatc tcgggtttcg cctcctatcg gatcaacgcg
     4081 cgttgggatg aggggcatgg cgcgggcaag gtgaccgtgc acgacctctt cgcggaaacg
     4141 ccagaagcct gggcgggaat ctggtcgctg gtgaccaatc aggatctcgt ggccagggcc
     4201 gtagggggtc ttcggccgcc ctgggatccg atcttcgacc tcctggcagg gacgcgcagg
     4261 gcccgtgctt atcgatatga cgcgctgtgg gtgcgtctga tggatgtcgc cgcagcgctg
     4321 gccgcccgct cttactcggc tccgctcgat ctggtcctgg gtgtcgagga cccgctaggc
     4381 gatgccaccg gctcttaccg cctacacgcc gactccgacg gggccgcatg cgcccccacg
     4441 accgacgagc ccactgtcct gctcgatctc gaggatctca gcgcgggtta catgggacgg
     4501 ccccggttca
//
LOCUS       DASTXO010000460         7476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_691124, whole genome shotgun sequence.
ACCESSION   DASTXO010000460 DASTXO010000000
VERSION     DASTXO010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7476
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691124"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VFU96_09690"
     CDS             <1..328
                     /locus_tag="VFU96_09690"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEU5113580.1"
                     /translation="VKRARREIEGKPHQPTIPTDEFLEATLDIWEIPSESAKRIGHPA
                     PFPVALPRRLIELYTYEGDLVLDPFMGSGTTAVAALRTGRHFVGVETDPDYVELARAR
                     LAEERR"
     gene            325..792
                     /locus_tag="VFU96_09695"
     CDS             325..792
                     /locus_tag="VFU96_09695"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEU5113581.1"
                     /translation="MRILAVCAGNICRSPAAEAAIQEAATGAGVDIETDSAGTGTWHI
                     GQPPHPECVAAGARAGLDVKGRARKVTPADFERFDIILAMDRANLRDLHELAPSREAR
                     ARTRLFRTYDPTASSDEVPDPWGGPEEGYDETVRIVRAAATGLVAEIREHSEV"
     gene            complement(793..1512)
                     /locus_tag="VFU96_09700"
     CDS             complement(793..1512)
                     /locus_tag="VFU96_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113582.1"
                     /translation="MRIGPLPDDFQFARDQLHQLAFYALAPARYAVEERMGLRATPGG
                     FGTPKYDGRVSRVDGNLLVQETGDNVATQAITTIRAAAEFLGVDYEVDWFTEFKDPLP
                     PMDPDRPLKVDEAPAAALGHWFAFGFETLEELRRHGVESDDVSEVQLWPEHFDPAIEM
                     GPESEGRKASYGASPGDRDHIEPYLYVSAWSDIDRDDPFWNDRNFNGASLPFTTLLEA
                     DDQGRVALEFFLEGYRILHSG"
     gene            complement(1509..2021)
                     /locus_tag="VFU96_09705"
     CDS             complement(1509..2021)
                     /locus_tag="VFU96_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU5113583.1"
                     /translation="MAAIDVVLLVFRLWVAIVMIAHGVNHGRTQEGTAKWFASKGFRS
                     PQLNARISAVNEILIGLALIAGLLTSVAAAGVVATMAVAFWSIHRFAGFFNFHRPDEG
                     WEYVATLSVIGTALAVLGPGRYSVDEALGIAGNLDGWIGLAIIGAGILLAAGQLAVFW
                     RKPAGSGGTV"
     gene            2130..2555
                     /locus_tag="VFU96_09710"
     CDS             2130..2555
                     /locus_tag="VFU96_09710"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU5113584.1"
                     /translation="MKTWAEGLVALGITLLLGAGLWLFSGRPLSAEPTDSSSPVSFDS
                     EAAARGEALAAATGCLACHNIDETAGTGPGWGGLAGSEVTLVSGETIVADDAYLFNSI
                     VDPVSQVVAGFEAVMPTTYSDSLSEAEIDDLVEYIKSLG"
     gene            2664..4496
                     /gene="dnaK"
                     /locus_tag="VFU96_09715"
     CDS             2664..4496
                     /gene="dnaK"
                     /locus_tag="VFU96_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEU5113585.1"
                     /translation="MARAVGIDLGTTNSVIAVLEGGDPTIIPNAEGGRTTPSVVSFKD
                     GEVLVGEVAKRQAITNPDQTVRSVKRHMGTSWSIEVDGKKLTAQEISARILMKLKRDA
                     ESYLGDKVTDAVITVPAYFNDAQRQATKEAGQIAGLEVLRIINEPTAASLAYGLDKEE
                     SDHTILVFDLGGGTFDVSVLEIGEGVFEVKATSGDTQLGGDDWDQKVIDWLVEGFKND
                     HGVDLGKDRMALQRLKEAAEKAKIELSTVAETEVNLPFITATSEGPLHLQRKLTRSEF
                     QKLTEELLERTKRPFEAAIKDSGVAVADIDHIVLVGGSTRMPAVQELVKSLTGKDPHR
                     GVNPDEVVSAGAAIQAGVLKGDVKDILLLDVTPLTLGIETKGGVMTRMIERNTTIPTK
                     RSEIFTTAENNQPEVEINVLQGERTMVADNKSLGKFRLTGIPPAPMGVPQIEVTFDID
                     ANGIVNVHAKDLATSKEQKMTITGGTALAQDDIDRMVKDAEAYAEEDAKRREAVETRN
                     QADQLVHQTEKLLEEQAENMNDEEKSGVETALGALRLTLEDENAETTEIRSKMDELIQ
                     ASQAMATRLYEQAAQQSQAAAAGDEAVTEDDDVVEAEIIDDDES"
     gene            4480..5058
                     /locus_tag="VFU96_09720"
     CDS             4480..5058
                     /locus_tag="VFU96_09720"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU5113586.1"
                     /translation="MTTSPEGHPEVVEAELVEAKQPAGAAAFGIDLPEDPEQAIDVLL
                     AHLEESREEASSYLDDLRRVAADFDNYRKRTTREQATTLDRAAERVVRELLPVLDTFD
                     AALGTSAESESERQLLSGMISTREQLLAALEKEGLEVVPTVGEVFDPEIHEPVGAPGG
                     TNDLVVASELRRGYRLRDRVLRPAMVMLEARE"
     gene            5055..6173
                     /gene="dnaJ"
                     /locus_tag="VFU96_09725"
     CDS             5055..6173
                     /gene="dnaJ"
                     /locus_tag="VFU96_09725"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020499452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU5113587.1"
                     /translation="MNRDWVDKDFYGILGVGSEATADEIKKAYRKLAQKHHPDANPDD
                     NQAEEKFKEISEAYATLSNAEQRKEYDQVRRMVETGGFAGYGAPSGGYGGQQVRVEDL
                     SDLLGGLGGLGDLFGFGSRGRAGPARGADAQADLSIGFDDAVAGVTTTLSVRGITTCS
                     HCGGNGAEPGTPVTTCPTCGGQGVVAQNQGFFSFSQPCPQCRGAGRIVETPCRVCRGA
                     GTETRTRDVKVRIPPGVQDGNTLRIPGKGGPGRNGGPNGDLLVKVHVQPSQTFGRKGN
                     DLTLTVPITYPEAVLGTKLEVPTLNGGVKLKVPAGTPSGKTFRVRGKGVTPDRGRTGD
                     LLVTVQVAVPSKVSKEEKKLLEDLAAMETDDVRSHLRT"
     gene            6181..6573
                     /locus_tag="VFU96_09730"
     CDS             6181..6573
                     /locus_tag="VFU96_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5113588.1"
                     /translation="MTETRRAVFVISVAAELAGMHPQTLRIYERKGLLEPARTPGGTR
                     RYSQEDIERLQLIQELTSQGLNLEGVRRVLALQEENRNLKRKVDKLRDKLDDIEEEME
                     QRLEEVHRSYRHDIVLRSQLRQVIGEDR"
     gene            6573..6845
                     /locus_tag="VFU96_09735"
     CDS             6573..6845
                     /locus_tag="VFU96_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113589.1"
                     /translation="MAESFSGPVRVLGDDGILLTTGSADLVTDSEMGGWRGTLETLQG
                     TAVAGKALVVELEIPGNGRGRAQLTPTGVDGDRAISEVVSLGPPFF"
     gene            6855..7466
                     /locus_tag="VFU96_09740"
     CDS             6855..7466
                     /locus_tag="VFU96_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113590.1"
                     /translation="MGAAADCEAVGAGFLGQPINTLTTLGFVAAGIIVIVRRQDMRWI
                     GIGLVSTGIGSFLFHGPMPPGNEWAHDVSLAWLVTMIAGWGTRWEWASRIPALVVIGA
                     VVAVFPLIADPLAAVMSAVAVISILIKDRSAATVGPLLLLASVAVIGRLGATGGPLCD
                     PDSLLQPHAIWHLGSALAVTWWAIGAGTTRSVRVADWLPRRSS"
BASE COUNT         1426 a         2416 c         2448 g         1186 t
ORIGIN      
        1 ggtcaagcga gcgaggcggg agatcgaggg caagccacac cagccgacga ttcccaccga
       61 cgagtttctc gaagccaccc tcgacatatg ggagatacct tcggagagcg cgaaacggat
      121 cggacatcct gccccgttcc ccgtcgccct cccccggcgc ctgatcgagc tctacaccta
      181 cgagggcgac ctggtgttgg acccgttcat gggctcgggt accacagcgg tggctgcgtt
      241 gcgaaccggc cgccacttcg tcggggtcga gaccgatccc gattacgtgg agctggcacg
      301 agctcgactc gccgaggagc gccgatgagg atcctcgccg tttgcgcggg caacatctgc
      361 cggtcgccgg ctgccgaagc ggcgatccag gaggcggcga ccggggcggg cgtcgacatc
      421 gagaccgatt cggccgggac cgggacctgg cacatcggtc aacctcccca ccccgagtgc
      481 gtggccgccg gcgcacgagc ggggctcgat gtcaagggac gcgcgcgcaa agtgacgccg
      541 gccgacttcg agcgtttcga catcatcctg gccatggacc gagccaatct ccgcgatctg
      601 cacgagctcg caccgtctcg cgaggcccgg gcacggaccc ggctcttccg aacctatgac
      661 ccgaccgcga gctcagacga ggtacccgac ccatggggtg gccctgagga gggttacgac
      721 gagaccgtcc gtatcgttcg ggccgcggca acgggtctgg tcgccgagat acgggagcac
      781 agcgaggtct gatcacccgg agtggaggat gcgatatccc tccagaaaga actcgagggc
      841 gacccggcct tggtcatctg cctcgagcag ggtggtgaac ggaagactgg ccccgttgaa
      901 gttgcgatcg ttccagaaag ggtcgtcccg gtcgatgtcc gaccacgccg agacatagag
      961 atagggctcg atgtggtccc ggtctccagg agaggccccg tagctcgcct tccgcccttc
     1021 ggactcaggg cccatctcga ttgccggatc gaaatgctcg ggccacagct ggacctcgga
     1081 tacgtcgtcg gattcgacgc cgtgccgccg aagctcctcg agagtctcga agccgaaggc
     1141 gaaccaatgt cccagagcgg cagccggagc ctcatccacc ttcagcggtc gatccgggtc
     1201 catcggaggc agcgggtcct tgaactcggt gaaccagtcg acctcgtagt cgacaccgag
     1261 gaactcggcc gcggcccgga tggtggttat ggcctgggtg gcgacgttgt ctccggtctc
     1321 ctgcaccagg agattcccat cgacccgtga gacacggccg tcgtatttcg gggtcccgaa
     1381 cccacccggg gtggcccgca atcccatccg ctcctctacg gcgtaacggg ccggagcaag
     1441 ggcgtagaag gccagttggt gtagttgatc acgggcgaac tggaagtcgt ccggaagtgg
     1501 cccgatcctc aaaccgtgcc tcccgatcca gccggcttgc gccagaacac agcgagctga
     1561 ccggcagcga gcaggatccc ggcgccgatg atggcaagac cgatccatcc gtccagattc
     1621 ccggcgatgc ccaatgcctc gtccaccgaa tagcgaccgg ggccgagcac ggccagtgcg
     1681 gtgccgatca cggacagggt cgccacgtac tcccatccct catcgggacg atggaagttg
     1741 aagaacccgg cgaatcggtg aatcgaccag aaggcgacgg ccatcgtcgc gaccacgccc
     1801 gctgcggcca cagaagtcag gaggccggcg atcagggcga gaccgatcag gatctcgttg
     1861 accgccgata tgcgggcatt cagctggggc gatctgaacc ccttcgaggc gaaccatttc
     1921 gccgttcctt cctgcgtcct gccatggttc accccgtggg cgatcatcac gatcgccacc
     1981 cacaacctaa acaccaacag gacgacgtcg atggccgcca tttctcctcc tcggaaacgt
     2041 gcgctctgcg cttcaacgac cccacatccg gagtcatttg gctcaggtaa ccataggatt
     2101 gccgactcga tgtctacggt tccggcggca tgaaaacttg ggcggaaggt ctggtcgcgt
     2161 tgggcataac cctcctgctc ggtgcgggtc tctggctgtt cagcgggcgg ccgctgtccg
     2221 ccgagccaac cgattcgagc tcgccggtct ccttcgacag cgaggccgcc gctcgtgggg
     2281 aggcgctagc tgcggcgacc ggctgcctcg cctgtcacaa catcgacgag accgcaggca
     2341 ccggccccgg gtggggaggt ctggccgggt cggaggtgac gttggtcagc ggggaaacga
     2401 tcgtcgccga cgacgcctat ctgttcaact cgatcgtgga cccggtctcc caagtcgtgg
     2461 ccgggttcga ggcagtgatg cccaccacct attcggacag cctcagcgag gctgagatcg
     2521 acgacctggt cgagtacatc aaatccctcg gctgacgccg atccgccccc tgccatattc
     2581 ccgtcttacg cggaatatga cacaacctca ctcaggttgt agcatcgtaa ccgactcaac
     2641 ctgaactcgg aggatcatta gaaatggcac gtgcagtagg catcgacctg ggcaccacca
     2701 acagtgtcat cgcggtgctg gaaggtggcg acccgaccat catccccaac gccgaaggcg
     2761 ggcggaccac tccgtcggtc gtctcgttca aggatggtga ggtgctggtc ggcgaagtcg
     2821 ccaagcgcca ggccatcacc aaccccgacc agaccgtgcg ctccgtgaag cgtcacatgg
     2881 gcaccagctg gtcgatcgag gtcgacggca agaagctgac ggcacaggag atctcggccc
     2941 gcatcctgat gaagttgaag cgggatgccg agtcctatct cggagacaag gtcaccgacg
     3001 cggtgatcac cgtccccgcc tatttcaacg acgcccagcg ccaggcaacc aaagaggcgg
     3061 gccagatcgc cggtctcgaa gtgctgcgca tcatcaacga gcccaccgcg gcgtcactcg
     3121 cctacgggct cgacaaggag gaatcggatc acacgatcct cgtcttcgac ctcggtggcg
     3181 ggacgttcga tgtctccgtg ctcgagatcg gcgaaggcgt gttcgaggtc aaggccacct
     3241 ccggtgacac ccagctcggt ggtgacgact gggaccagaa ggtgatcgac tggctcgtcg
     3301 aggggttcaa gaacgaccat ggcgtcgatc tgggcaagga tcgaatggct ttgcagcgtc
     3361 tgaaggaagc ggcggaaaag gccaagatcg agctgtccac cgtcgccgag accgaggtga
     3421 acctgccttt catcacggcg acttccgagg ggcccctcca tctccagcgc aagctgaccc
     3481 gttctgagtt ccagaagctc accgaggagc tgttggagcg caccaagcgg ccgttcgaag
     3541 cggcgatcaa ggattccggc gtcgccgtcg cggacatcga ccacatcgtg ctggtcggcg
     3601 ggtcaacccg tatgccggcc gtccaggagt tggtcaagag cctcaccggt aaggaccccc
     3661 accgcggcgt caaccccgac gaggtcgtct ctgccggggc cgcgatccag gccggtgtcc
     3721 tcaagggcga tgtcaaggac atcctccttc tcgacgtcac gcctctgacc ctcggcatcg
     3781 agaccaaggg cggcgtcatg acccggatga tcgagcggaa cacgacgatc cccaccaaga
     3841 ggtctgagat cttcaccacg gccgagaaca atcagccgga ggtcgagatc aacgtgctcc
     3901 agggcgagcg gaccatggtc gccgacaaca agagcctcgg gaagttccgc ctcaccggta
     3961 tcccaccggc accgatgggt gtgccccaga tcgaggtcac cttcgacatc gacgccaacg
     4021 ggatcgtgaa cgtgcatgcc aaggaccttg ccaccagcaa ggagcagaag atgacgatca
     4081 ctggcggcac cgcactggct caggacgaca tcgaccggat ggtcaaagac gccgaggcct
     4141 atgccgaaga ggacgccaag cgccgcgagg cggtggagac ccgtaaccag gcggaccaac
     4201 tcgtccatca gaccgagaag ctcctcgagg agcaggccga gaacatgaac gacgaggaga
     4261 agtcaggtgt cgagaccgca ctcggcgcac ttcggctgac tctggaagac gagaacgccg
     4321 agaccacaga gatcagatcc aagatggatg agctgatcca ggcgagccag gccatggcga
     4381 cccggctcta tgagcaggcc gcccagcaga gccaggctgc cgcagccggg gacgaggcgg
     4441 taaccgagga cgacgatgtc gtcgaagcgg aaatcatcga tgacgacgag tcctgaaggg
     4501 catccggagg tcgtcgaggc tgagttggtc gaggccaagc agccggcagg cgccgccgcc
     4561 ttcggcatcg acctgcctga ggatcccgag caggcgatcg acgtgctgct cgcccacctc
     4621 gaggaatcga gggaggaggc ctcttcgtat ctcgacgacc tgaggagggt tgcggccgac
     4681 ttcgacaact accgcaagcg gaccacgcgc gagcaggcaa cgactctgga ccgtgcggct
     4741 gagcgagtgg tccgagagct cttgccggtt ctggacacgt tcgacgccgc cctgggcaca
     4801 tcggccgaat ccgagagcga gagacagctt ctctccggga tgatcagcac ccgtgagcag
     4861 ctactcgccg ctctggagaa agagggcctg gaggtcgtgc ccaccgtcgg cgaggtcttc
     4921 gaccctgaga tccacgagcc ggtcggggcc ccggggggca ccaacgatct ggtggtggcg
     4981 tccgagttgc gtcgtgggta ccggctccgg gaccgggtcc tccgtcctgc gatggtgatg
     5041 ctggaagccc gagagtgaac cgggactggg tcgacaagga cttctacgga atcctcgggg
     5101 tcggctccga ggccacggcc gacgagatca agaaggcgta tcgcaagctc gctcagaagc
     5161 atcaccccga cgccaacccc gatgacaacc aggccgagga gaagttcaag gagatctccg
     5221 aggcgtacgc caccctctcc aacgccgagc agcggaagga atacgaccag gtccgccgca
     5281 tggtggagac cggcggcttt gccgggtacg gcgctccgag cggcggctac ggcggtcaac
     5341 aggttcgggt cgaggacctc tccgatctgc tcggtggtct tggtggtctc ggcgacctgt
     5401 tcggtttcgg gtcccgggga cgggcgggcc cggcccgggg agccgatgcg caggcggacc
     5461 tgagcatcgg tttcgacgac gcggtcgccg gggtgaccac cactttgagc gtgcgtggta
     5521 tcaccacctg ctcacactgc ggcgggaatg gagcggagcc gggaacaccg gtgaccacct
     5581 gcccaacctg cggtggtcag ggtgtggttg cccagaacca gggcttcttc tccttctccc
     5641 agccttgtcc ccagtgccgc ggggccggtc ggatcgtcga gactccgtgc cgggtgtgcc
     5701 gcggggcggg aaccgagacc aggacccgcg acgtgaaggt gcgcatcccg ccgggtgtcc
     5761 aggatgggaa cacccttcgc atcccgggca agggcggccc cggccgcaac ggtgggccca
     5821 acggtgatct cctggtcaag gtccacgtcc agccgagcca gacattcggc aggaagggaa
     5881 atgacctgac cctcaccgtc ccgatcacgt atcccgaggc ggtgttgggg accaagctgg
     5941 aggtcccgac gctcaatggc ggtgtgaagc tcaaggtccc agcggggacg ccgtcgggga
     6001 agacgttccg ggtccgggga aagggggtca cccccgaccg aggtcgcacg ggagatctgt
     6061 tggtcaccgt gcaggtcgcc gtcccctcca aggtgtccaa ggaagagaag aagctcctcg
     6121 aggacctggc ggccatggaa accgacgatg tccgatccca tctgagaaca tgaggccgac
     6181 gtgaccgaaa cgagaagggc cgtattcgtc atctccgttg ccgccgagct cgcggggatg
     6241 catccgcaga cactgcgcat ctacgagcgc aaaggcctgc tcgaaccggc acgcacaccc
     6301 gggggcaccc gtcgctattc gcaggaggac atcgaaaggc tgcagctgat ccaggagctg
     6361 accagccagg ggctcaacct cgaaggtgtc cggcgagtcc tcgccctcca ggaggagaac
     6421 cgcaacctca agaggaaggt cgacaagctg cgcgacaagc tcgacgacat cgaggaggag
     6481 atggagcaga ggctcgaaga ggtgcaccgc tcatatcggc acgacatcgt cctccgctcc
     6541 cagctccgcc aggtcatcgg agaggaccgc tgatggccga gtccttctcg ggcccggttc
     6601 gtgttctggg cgacgacggg atcctcctca ccactggttc ggcggacctg gtcaccgatt
     6661 ccgagatggg aggttggagg ggcaccctgg agacactcca gggaacggct gtagcgggca
     6721 aggccctggt agtcgagctg gagatcccgg ggaatggacg gggccgcgct cagctcaccc
     6781 ccaccggggt ggacggcgac agggccatct ccgaggtggt cagcctcggc ccacccttct
     6841 tctaagggcc cggcttgggc gccgccgccg attgtgaggc cgtgggggcg gggtttctcg
     6901 ggcagccgat caacaccctg accacgctcg ggttcgtcgc cgccgggatc atcgtcatcg
     6961 tccggcgcca ggacatgcgc tggatcggga tcggtctcgt cagcaccggg atcggctcgt
     7021 ttctctttca cggcccaatg ccgccgggca acgaatgggc ccacgacgtc agcctggcct
     7081 ggttggtgac gatgatcgcc ggatggggca ctcgttggga gtgggcctcc cgcattcctg
     7141 cattagtcgt catcggcgcc gtcgtagccg tcttcccctt gatcgccgac cccctcgccg
     7201 ccgtcatgag cgcagttgcc gtgatatcga tcctcatcaa agaccgctcc gcagcgacgg
     7261 tcggcccctt gttgcttctg gcgtcggtag ctgtcatcgg ccgactcggc gccaccggtg
     7321 ggccgctgtg tgaccccgat tcgctgttgc aaccgcatgc catctggcac ctgggctccg
     7381 ccctcgccgt gacctggtgg gcgatcggcg caggcaccac acgctccgtt agggtcgccg
     7441 actggttgcc aaggaggtcg tcatgatcca ggagtt
//
LOCUS       DASTXO010000461         2600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_248302, whole genome shotgun sequence.
ACCESSION   DASTXO010000461 DASTXO010000000
VERSION     DASTXO010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248302"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VFU96_09745"
     CDS             <1..249
                     /locus_tag="VFU96_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113591.1"
                     /translation="QTETLSLPVIVMAAMACLLSLAQRSLSTPARFVRRRTGESMTVF
                     DGDRSWDRTELLQTWEKPLRLLTWTTITLAIGLLLTHL"
     gene            complement(266..1531)
                     /locus_tag="VFU96_09750"
     CDS             complement(266..1531)
                     /locus_tag="VFU96_09750"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEU5113592.1"
                     /translation="MSLSSLHQDPGQAHADITSHDTFVRSVPHATFARMRREEPVAWV
                     EEEDGHGFWALTKHADISAASRDFGRFTASRGIRIEQMAPDELEARRTMMEFDPPEHA
                     RLRRLVQPGFTPKVVATYERAFRILAGDVLDRVLALGRFDFVTEIARELPIRLLCRLL
                     GVPESDAQQLVTWGDQMISNADPEYTPVVIDKMDTDEYRLLPFRSPAALEVFRYAEEV
                     ARERRDHPADDIITVLLTAEPDGQPLTDLEFKNFFTLMMVAGNETTRHTVSHGLIYLV
                     DHPDQMEQLRDDPAGLSATATEEILRASSVTMHFRRTATEEIVMRGRTILPGDRVVMW
                     YPSANHDEEVFDRPFTFDIRRHPNDHLAFGTGRHVCLGASLARLEVRVVFEELLRRVA
                     SLEITGPPDRLRSNFINGIKHLPVEVTTA"
     gene            1576..2457
                     /locus_tag="VFU96_09755"
     CDS             1576..2457
                     /locus_tag="VFU96_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU5113593.1"
                     /translation="MAEYRRILLDGVVTRVIRDGEHLLAGDGRSVSAELAVHLPPVEP
                     SKIIAVHLNYRSRVDEFMTSLPPAPTFFHKPTTALNSHRGSVVRPAGCRFLNYEGEIA
                     IVIGETCRNVSRSEAESRIAGYTIANDFGIHDFRDTDAGSMLRVKGSDTLCPIGPGLV
                     TDWEFRGKRISTLVNGALRQQGSTDEMEWDMGYLVADLARTITLLPGDVILTGTPAGS
                     RPVEPGDVVTVVVEGLGELTNTIVEGPAGIDANIGAQPSESEEVVSTALGGDWELRGI
                     RAPRGAGSAHYHSELDA"
     gene            2450..>2600
                     /locus_tag="VFU96_09760"
     CDS             2450..>2600
                     /locus_tag="VFU96_09760"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEU5113594.1"
                     /translation="MPEAPWRVVALLRGVNVGGVKVPMGDLRSLLAREDIGEIRTHLQ
                     SGNVIV"
BASE COUNT          467 a          802 c          878 g          453 t
ORIGIN      
        1 caaacagaga cgctgagcct gccggtcatc gtcatggccg cgatggcttg cctgctcagc
       61 ctggcgcagc gatcgttgag caccccggct cgctttgtca ggcggcgcac cggtgagtcg
      121 atgactgtgt tcgacgggga ccggtcctgg gacagaaccg agctcttgca gacatgggag
      181 aagccacttc ggctcttgac ctggacaacc atcacactcg ccatcggtct gctgctgacc
      241 cacctctgaa ggcgagcacg acgcatcagg ccgtcgtcac ttctacgggg agatgcttga
      301 tgccgttgat gaagttggac cgaagacgat cgggcggccc ggtgatctcg agggaagcga
      361 cccgcctcaa cagctcctcg aacaccactc gcacctcgag acgggccaaa gaggcgccaa
      421 ggcagacgtg gcggcccgtg ccaaaggcga gatggtcgtt gggatggcgc cggatgtcga
      481 aggtgaacgg gcggtcgaac acctcctcgt cgtgattggc cgacgggtac cacatgacca
      541 cccggtctcc ggggaggatg gtccgccctc gcatgacgat ctcctcggtg gcggtgcgcc
      601 ggaaatgcat ggtcacagac gaggctcgca ggatctcctc ggtggcggtc gccgaaagtc
      661 ccgccggatc gtcccgcagc tgctccatct ggtcgggatg gtcgacgagg tagatcaggc
      721 cgtggctgac ggtgtgcctc gtcgtctcgt tcccggccac catcatcagg gtgaagaaat
      781 tcttgaactc gaggtcggtg agcggctggc cgtctggctc ggcggtgagg agcaccgtga
      841 tgatgtcgtc ggccgggtgg tcgcgacgtt cccttgccac ctcctccgca tatcgaaaga
      901 cctcgagcgc ggccggggat cggaagggga gcagccggta ctcgtcggtg tccatcttgt
      961 cgatgaccac gggggtgtac tcggggtcgg cgttggagat catctgatct ccccaggtca
     1021 ccaattgctg cgcgtccgac tccggcacgc cgagaaggcg gcaaagaagc ctgatcggca
     1081 gctcacgggc gatctcagtc acgaaatcga accgcccgag ggcgaggaca cggtcgagga
     1141 catcaccggc caggatgcgg aacgctcgct cataggtcgc caccactttc ggagtgaatc
     1201 cgggctggac gagccgccgc agcctggcgt gctccggggg gtcgaactcc atcatggtgc
     1261 gccgcgcctc gagctcgtcc ggcgccatct gctcgatcct gattcccctc gaggcggtga
     1321 atctcccgaa gtcgcggcta gctgccgata tgtcggcgtg cttggtcagc gcccagaagc
     1381 cgtgcccgtc ttcttcctcg acccaggcca cgggttcctc gcggcgcatc cgggcgaatg
     1441 tggcatgagg caccgagcgc acgaaggtgt cgtgtgacgt gatgtcggcg tgggcctgac
     1501 ccggatcctg gtgaagggag ctcagggaca tttcccgaca tgatcgcagg accggtatgg
     1561 gtaccgtgtg gccggatggc ggaataccga aggatcctcc tcgacggagt ggtcacccgc
     1621 gtcatcaggg acggtgagca cctgctcgca ggtgatgggc gctcggtcag cgccgagctt
     1681 gccgtccacc tcccgccggt cgagccgtcg aagatcatcg cggttcacct caactaccgc
     1741 agccgtgtcg acgagttcat gaccagcctc cccccggcgc cgactttctt ccacaagccg
     1801 acgaccgcgc tgaactccca tcgcggcagt gtggttcggc cggccggctg ccggtttctc
     1861 aactacgagg gtgagatcgc catcgtcatc ggggagacct gtcgaaatgt ctcgaggtcc
     1921 gaggctgaat cgcgaattgc cggatacacg atcgccaacg acttcggaat ccacgatttc
     1981 cgcgacacgg atgccggctc gatgctcagg gtcaagggat ccgacacact ttgcccgatc
     2041 gggccggggc tcgtcaccga ctgggagttc agggggaaac ggatctcgac cttggtgaat
     2101 ggggccctcc gacaacaggg gtcgactgac gagatggaat gggacatggg ttatctcgta
     2161 gcggacctgg cacgcaccat cacgctcctg cccggggacg tcatcctgac cgggacaccg
     2221 gcggggtcga ggccggtaga accgggtgac gtcgtaacgg tcgtggtcga gggactgggt
     2281 gagttgacca acacgatcgt cgaagggcca gccggcatcg acgcaaacat cggggctcag
     2341 ccaagcgaat ccgaggaggt ggtctcgacc gccctcggag gggattggga gctccgtggg
     2401 atcagagcgc cccgtggcgc cggatctgcg cactaccact cggagctcga tgcctgaagc
     2461 gccgtggcgt gtcgtggcac tgctccgcgg agtcaacgtg ggaggcgtca aggtgccgat
     2521 gggggacctc cgatcgctgc tcgcccggga ggacataggc gagatccgga ctcacctcca
     2581 gagcgggaat gtgatcgtga
//
LOCUS       DASTXO010000462         2362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_174752, whole genome shotgun sequence.
ACCESSION   DASTXO010000462 DASTXO010000000
VERSION     DASTXO010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174752"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="VFU96_09765"
     CDS             complement(<1..1164)
                     /locus_tag="VFU96_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HEU5113595.1"
                     /translation="MSNFVCIHGHFYQPPRENPFTGMVPEQPTAAPWHDWNERITEES
                     YAANARARILDGNGEVSRTINTYESISFDFGPTLLSWLEDAAPDTYRAIIEADDASAR
                     RFAGHGSAMAQAYNHAILPLSNQRDKMTQVRWGIADFEHRFGRRPEGMWLPETAADTE
                     TLDVLSREGIGFTVLSPYQAAAVEGSDGSWSDVGGGRIDTRVPYLIDLGEDRSIAVFF
                     YDGPLSREIAFDGLLNDGHALAHRLVSAAGDPGESPRLAHVATDGETYGHHHRHGEMA
                     LATALETIEAGSSARLTNYAEFLSLVPPTARAAIIEDSSWSCAHGVERWRADCGCHTG
                     REPTGHQRWRGPLRSALDWLRDELTGPFESVGADLLEDPWRARDAYISVVLGGS"
     gene            complement(1194..>2362)
                     /gene="glgB"
                     /locus_tag="VFU96_09770"
     CDS             complement(1194..>2362)
                     /gene="glgB"
                     /locus_tag="VFU96_09770"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HEU5113596.1"
                     /translation="HGLGLFDGTHLYEHADPRRGFHPDWDTYIFNYGRAEVPAFLISN
                     ALFWLDRYHADGLRVDAVASMLYLDYSRKAGEWVPNRHGGREDLEAIAFIRRLNETVY
                     QEYPAVQTIAEESTSWPMVSRPTFLGGLGFGLKWDMGWMHDTLVHLSREAVHRSYHHH
                     ELTFRAVYAFSENFMLPISHDEVVYGKRALVEKFPGDDWQKMATLRLLLAYMWTQPGK
                     KLLFMGSESAQRTEWNHDEQLDWSLLELPPHSGIHRLVTDLNRVYAVEPALHELDCDP
                     AGFDWAVASDGANSVLAYFRHSGAGDTVLVAVNLTPVPRHVYRVGVPVPGRWVEILNT
                     DAEPYGGSGQGNFGTVETGPLAVPHHGRPHSIDLTLPPLGVVMLRHEPMSGKSG"
BASE COUNT          437 a          743 c          775 g          407 t
ORIGIN      
        1 cgatcctccg agaacgaccg agatataggc gtccctcgcc cgccatggat cctcgagcag
       61 gtcggcgccg acggactcga acggacccgt cagctcgtcg cgcagccagt cgagagccga
      121 gcgaagcggg ccacgccacc gctggtgccc cgtcggctcg cgtccggtgt gacagccaca
      181 gtcggcccgc cagcgttcga cgccgtgggc acaactccaa gaggagtcct cgatgatcgc
      241 ggcccgcgcg gtcggcggga ccaacgagag aaactccgcg taattggtga gacgggctga
      301 agatccggcc tcgattgtct ccagagccgt ggccagggcc atctcaccgt gccgatgatg
      361 gtgaccatag gtctcgccat cggtcgcgac gtgagccagt ctgggggact ctccgggatc
      421 tcccgcagcc gacaccagac gatgagccag cgcatgtccg tcgttcagca gtccatcgaa
      481 ggcgatctcc cgggacaacg gcccgtcata gaagaaaacg gcgatagacc gatcttcacc
      541 caggtcgatc agatagggca cccgggtgtc gatccgccct ccacccacgt cggaccagga
      601 cccatcgctc ccctcgaccg cagccgcctg atagggagag agcacggtga acccgatccc
      661 ctctcgggaa aggacgtcga gagtctcggt gtccgctgcc gtctcaggca gccacatccc
      721 ctcgggtcgt cgcccgaacc tgtgctcgaa atcggcgatg ccccaccgca cctgagtcat
      781 cttgtctcgc tggttggaaa gcgggaggat cgcgtggttg taggcctggg ccatggccga
      841 gccgtggccg gcgaacctcc gggcggacgc gtcgtcggct tcgatgatgg cccgatatgt
      901 gtccggggcg gcgtcctcca gccagctgag cagtgtcggc ccgaaatcga agctgatcga
      961 ctcgtaggta ttgatcgtcc tcgacacctc tccgttgccg tcgaggatcc ttgcccgagc
     1021 gttggcggcg taggactcct cggtgatgcg ctcgttccag tcgtgccatg gggcggcggt
     1081 tggctgctcg gggaccattc cggtgaatgg gttctcccgc gggggctgat agaagtgccc
     1141 gtggatacag acgaagttgc tcaagatgga gtcaactcaa gaacgcctgg gagctagccg
     1201 ctcttccccg acatcggctc atgccgaagc atcaccacac caaggggagg gagagtgagg
     1261 tcgatgctgt gcgggcggcc gtggtggggc accgccagcg gcccggtctc gacggttccg
     1321 aagtttccct gcccgctgcc cccataaggt tccgcgtcgg tgttgaggat ctcgacccag
     1381 cgaccgggga cgggcacacc tacgcgatac acatgtcggg gaaccggtgt gagattgacc
     1441 gcgacgagga ccgtgtcacc tgcccccgag tggcggaagt aggccaggac gctgtttgcc
     1501 ccatcactgg ccacggccca atcgaagccc gccggatcac agtcgagctc gtggagcgcc
     1561 ggctccacgg cgtagacgcg attgagatcg gtgaccagcc tgtggatgcc ggagtgggga
     1621 ggcagctcga gcaggctcca gtcgagctgc tcatcgtgat tccattccgt gcgttgcgcg
     1681 gactccgagc ccatgaacag gagcttcttc cccggctgtg tccacatgta ggcgagaagc
     1741 aggcgaagag tggccatctt ctgccagtcg tccccgggga acttctcgac cagagcgcgt
     1801 ttgccgtaca cgacttcgtc gtgggagatg ggcagcatga agttctcgct gaaggcgtat
     1861 accgcccgaa aggtcagctc atggtggtga taggagcgat gcacggcctc ccgtgagaga
     1921 tggacgagcg tgtcgtgcat ccaccccatg tcccacttga ggccgaagcc gagaccgccg
     1981 aggaaagtag ggcgtgacac catcggccag gacgtggatt cctcggcgat cgtctgcacc
     2041 gcgggatatt cctgatagac ggtttcgttc agccgtcgga tgaaggcgat cgcctccagg
     2101 tcctcccgcc cgccatgacg attggggacc cactcacccg ccttgcgcga gtagtcgagg
     2161 tagagcatcg aggccacagc atcgacgcgt aaaccgtctg cgtgataacg gtctagccag
     2221 aagagtgcgt tgctgatgag gaaggcgggc acctcggcgc gtccgtagtt gaagatgtag
     2281 gtgtcccaat cgggatgaaa gccccgcctc ggatcggcgt gctcatagag atgcgtgccg
     2341 tcgaagagcc cgagaccgtg cg
//
LOCUS       DASTXO010000463         5519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_985326, whole genome shotgun sequence.
ACCESSION   DASTXO010000463 DASTXO010000000
VERSION     DASTXO010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985326"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /gene="ahcY"
                     /locus_tag="VFU96_09775"
     CDS             complement(<1..1075)
                     /gene="ahcY"
                     /locus_tag="VFU96_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012673819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEU5113597.1"
                     /translation="MNYDISNPALAEAGARRVEWAGRRMQVLATVRERFAKEKPLDGL
                     VVAACLHVTAETANLMLALRDGGAEPLLCASNPLSTQDDVAAALVAEGIPVFAIRGED
                     ADTYYKHIHAVLDHAPAITMDDGADLATLLHTDRRDIEVIGSTEETTTGVIRLKAMAA
                     DGTLRVPVVAVNDSATKHLFDNHYGTGQSAIDGIIRATNILLAGQNVVVIGYGDCGRG
                     VANRADGLGANVIVVEIDPVRALSAAMDGFRVMTGADAARVGDVFITVTGNKHVLRME
                     HFDVMKDGVILANAGHFDIELDLAALRAASVARREIRDNLEEFEAQDGRRLLVIAEGR
                     LVNLGAAEGHPADVMDMSFSNQAL"
     gene            complement(1077..2078)
                     /locus_tag="VFU96_09780"
     CDS             complement(1077..2078)
                     /locus_tag="VFU96_09780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HEU5113598.1"
                     /translation="MSEMLDMIRTLGDQIRWARDLEAPDLPVNSEVLVGGMGGSGIAG
                     DFGAALTSRSEGRVSVHKGYSPLPGWAHRVKPTVIAASYSGNTEETLDLVDAAAAAGL
                     RIVTVTTGGQLGELSARNTWPTITVPIGLQPRAAVGYLAGAVSRLLVTLGVLPDQTTA
                     LDEAADLVDAAATEGSDTWDIANGLAKGLVGRVTVIYGGGPISGTAAQRWKTQINENA
                     KMPAWWSVLPELDHNEIVGWETLPSLTRDIIGIVSLTDIGDHARVRDRLDHTSQLTEV
                     AVPWIGEVRARGESEFARLMSLTVSGDLVSYMLAGEAGVDPVPVETIEKLKKLLVKD"
     gene            complement(2071..2250)
                     /locus_tag="VFU96_09785"
     CDS             complement(2071..2250)
                     /locus_tag="VFU96_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEU5113599.1"
                     /translation="MSLIDPQLAAILVCPVDKADLTEDEAASRLVCSACGRRYPVRDG
                     IPVMLVEEAEGGPDE"
     gene            complement(2247..3590)
                     /gene="manB"
                     /locus_tag="VFU96_09790"
     CDS             complement(2247..3590)
                     /gene="manB"
                     /locus_tag="VFU96_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006239414.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEU5113600.1"
                     /translation="MSPDSVFKAYDVRGRTDNGELTPDLYRKIGVAFVSLVDAGEIAV
                     GRDCRESSEALFDALVDGVTAAGADVVDLGLVPTDLVYFYSGRHSVPGAMITASHNPP
                     QYNGLKLCRAGAAPVGADTGLEDIKKVVVEGDEPHAAVPGSVRSVDATGDYVDHLLAI
                     VDGSSIGSLRVAVDGGNGMAGVTIEEVFARLEATLDGIYLEPDGTFPNHPADPIQPEN
                     LEDLIAMVAEGGHDLGVAFDGDADRAFFIDDRAEAVSGSTVTALIARWMLERHPGASI
                     IHNLITSRAVPETVRAAGGTPIRTRVGHSYIKQVMAESGAVFGGEHSGHYYFADNYRA
                     DSGMLAMLILMRVISEAGQPLSALRRDVEPYRASGEINFVVSDPEAAMKRVEAMYPDA
                     DKDHVDGMTIDLGNVWFNLRPSNTEPLLRLNVESADESAMQVTVAELAATLEEER"
     gene            3648..4385
                     /locus_tag="VFU96_09795"
     CDS             3648..4385
                     /locus_tag="VFU96_09795"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5113601.1"
                     /translation="MRPTFSIVVPVYNEADFIPKAVPALIEQMDGLGEPYRILIVENG
                     STDGTAAAAAQAAAHSPVTVMSLDVADYGAAMRHGFLAADGSWVVNFDIDYFSADFLK
                     RVLALDDVDLVIASKRDPGSEDRRPLLRRFATWVFNLLLRSILDSRVSDTHGMKAFRH
                     DLVSTLAPSVRSRQDLFDTELVIRAERAGYRIEEVPVVVEEMRVARSSLVKRVPRTIR
                     GLLRIRRTLADEALDLAPAHTPDSDAD"
     gene            complement(4341..>5519)
                     /locus_tag="VFU96_09800"
     CDS             complement(4341..>5519)
                     /locus_tag="VFU96_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113602.1"
                     /translation="DSTEFPAWAAIPVGIAGALVIGLTFVWESSMSTRTFLTGATVCV
                     VVLGALSGVTFVDGWAKWNYAGYENKPKWPEYEALMAEIDTLPEGRVHWEAANKELGV
                     YGTPMSPMLIPYWTEGSHPSMEGLFFESSLTTPFHFINSSEMSERPSNPIPGLQYHTG
                     EMERGLKHLELFGVDYYVSVTPEATERADGIPEMSKLATTGPFSIYRLPETELVEVAS
                     FLPSVYEVPEHGLLDSLTGSDTVTGSDGEELPSFFDMALTWYEDVDNLDRLVVTDGPA
                     EWPRIESIEQRPDVALDAPADAVSDIVVENHRISFNTTAVGLPHLIKVSYFPNWTATG
                     ADGPWRATPSLMVVVPTSEQVVLEFQDTWAETGGKIATLIGVLGLIGVGIWGVRRRQV
                     "
BASE COUNT          963 a         1823 c         1720 g         1013 t
ORIGIN      
        1 cgagtgcctg gttggagaat gacatatcca tcacgtcggc ggggtgcccc tcggccgctc
       61 ccagattgac caaccgccct tcggcgatga ccaggagacg tcgtccgtcc tgcgcctcga
      121 actcttcgag attgtcgcgg atctcgcggc gcgccactga tgcggcgcga agcgccgcca
      181 gatccagctc gatgtcgaaa tgcccggcat tggccaggat cacgccgtcc ttcatcacgt
      241 cgaaatgctc catgcgaagc acatgcttgt tgcccgtcac cgtgatgaac acatcgccaa
      301 cccgcgccgc gtccgcaccg gtcatgaccc ggaacccgtc cattgccgcc gacagcgcgc
      361 ggacggggtc gatctcgacg acgatcacgt ttgctccgag cccatcagct cggttggcca
      421 cacctcgtcc gcagtcgcca tatccgatca cgaccacgtt ctgcccggcg aggaggatgt
      481 tggtggccct gataatgccg tcgatcgccg actgcccggt cccgtagtgg ttgtcgaaca
      541 gatgcttggt tgccgagtcg ttgactgcaa ccacaggaac gcgaagcgtt ccgtcagctg
      601 ccatcgcctt gaggcggatg actccggtgg tcgtctcctc cgtggatccg atgacttcga
      661 tgtcccgacg atcggtatgg agcagggtgg ccagatcggc gccgtcgtcc atcgtgatcg
      721 caggagcgtg gtcgagcact gcgtggatgt gcttgtaata ggtgtcggcg tcctcgccgc
      781 ggatagcgaa cacagggatg ccctcggcga cgagagccgc cgccacgtcg tcctgggtgg
      841 acagcgggtt ggaggcgcag agcagcggct cggccccacc gtcgcgtagc gccagcatga
      901 gattggccgt ctcggcagtg acatggaggc atgcggcaac gacaagccca tcgagaggct
      961 tctccttggc gaaccgctcc cgcacggttg ccaagacctg catgcggcgc ccggcccatt
     1021 cgactcgtcg ggcgccggcc tcggccagcg cgggattgga aatgtcgtag ttcatctcag
     1081 tccttcacca gtagtttctt cagcttctcg atggtctcga ccggtaccgg gtcgacaccg
     1141 gcctctccgg ccagcatgta agacacgagg tccccgctga ccgtgagtga catcaatcgg
     1201 gcgaattcgg actctccccg cgccctgacc tctccgatcc agggcaccgc gacctcggtg
     1261 agttgcgacg tgtggtcgag gcggtctcgc acccgtgcgt ggtcaccgat gtcggtgagc
     1321 gacacgatgc cgatgatgtc ccgcgtcaac gagggcaagg tctcccaccc cacgatctcg
     1381 ttgtggtcga gctcgggaag caccgaccac caagccggca tcttggcatt ctcattgatc
     1441 tgagtcttcc agcgttgggc ggccgtgcca ctgatcggac cacccccgta gatgacggtg
     1501 acccggccca ccagtccctt ggctagccca tttgcaatgt cccaggtatc cgagccttca
     1561 gtagcggcgg cgtcgaccag gtccgctgcc tcgtcgagag ctgtcgtctg atcggggagg
     1621 acacccaggg tgacgaggag cctggacaca gcaccggcca agtagccgac tgcagcccgg
     1681 ggctgaagcc cgattgggac cgtgatcgtg ggccaggtgt ttcgggccga gagctcgccc
     1741 agctgtcccc cggtggtcac ggtcacgatc cgcaagccgg cagcagcggc ggcatccacg
     1801 aggtccagtg tctcctctgt gttccccgag tacgaggccg cgatgacagt gggcttcacc
     1861 cggtgtgccc accccggaag cggcgaatac cccttgtgaa ccgaaacccg accctcgctc
     1921 cggctggtca gggctgcgcc gaagtctccg gcgatcccgg acccacccat cccgccgacc
     1981 agcacctcgg agttgactgg cagatcggga gcttcgaggt cgcgcgccca acggatttga
     2041 tcacccaagg tccggatcat gtccagcatc tcactcatca ggaccccctt cggcttcctc
     2101 tacaagcatc actgggatcc catcacggac cgggtatctg cggccgcacg ccgagcacac
     2161 cagtcgagac gccgcctcgt cctcggtgag gtcggccttg tcgaccgggc acaccaggat
     2221 cgcagccagc tgtggatcga tcaggctcat cgctcctcct cgagagtcgc cgccaactcg
     2281 gccacggtca cctgcatggc cgactcgtcg gccgactcga cgttcaacct cagcaacggc
     2341 tccgtgttcg acggccgcaa attgaaccag acgttcccga ggtcgatcgt catcccgtcg
     2401 acatggtcct tgtcggcgtc gggatacatc gcctcgaccc gcttcatggc ggcttccggg
     2461 tcggatacga cgaagttgat ctctcccgat gctcgatatg gctcgacatc tcggcgaaga
     2521 gcagagagag gctgccccgc ctcggagatc acgcgcatga ggatcagcat tgccagcatc
     2581 ccggaatcgg cccggtagtt gtccgcgaag tagtaatgac cgctgtgctc cccaccgaac
     2641 acagcgcccg actccgccat gacttgcttg atgtagctgt gtccgactct cgtccgaatc
     2701 ggggtccccc cggcggctct gaccgtctct gggaccgccc gtgaggtgat caggttgtgg
     2761 atgatcgaag ccccgggatg tcgctcgagc atccagcggg cgatcaaggc tgtgacggtg
     2821 cttccgctca cggcttcggc acggtcgtcg atgaagaatg cgcgatcggc gtcaccatcg
     2881 aaggcgaccc ctaggtcgtg ccctccttcg gcgaccatcg cgatcagatc ctccaggttc
     2941 tcgggctgga tgggatccgc cgggtggttg gggaaggtgc cgtcgggctc caggtagatg
     3001 ccgtccagcg tcgcctctag tctggcaaaa acctcctcga tcgtcactcc tgccatcccg
     3061 ttccctccgt cgaccgctac ccgcagggag ccgatcgatg acccatcgac tatcgcgagg
     3121 aggtgatcca cgtaatcgcc ggtggcgtcg acggagcgca ccgagccggg caccgcggcg
     3181 tgaggctcat ctccttcgac gactaccttt ttgatgtcct ccaatccggt gtctgcgccg
     3241 actggcgcgg ctccggcccg gcacagtttc aggccgttgt attggggagg gttgtgggag
     3301 gcggtgatca tcgccccggg cacggagtgt ctccccgagt agaagtagac caggtcggtc
     3361 gggacaagcc caagatccac gacatcggcg ccggcggcgg tgactccgtc caccaacgca
     3421 tcgaacaggg cctcggacga ctcccgacag tcccgtccca cggcgatttc gcccgcatcg
     3481 accagcgaca cgaaggcaac gccgatcttc cggtagagat ccggggtcag ttccccgttg
     3541 tccgtgcgcc cgcgcacgtc ataggccttg aacacggaat cggggctcat cggcgccgga
     3601 gtctaggggt gggcctatcc catgctcatt tctacacttg cgcccggatg cgccccacct
     3661 tctcgattgt ggtccccgtg tacaacgagg ccgatttcat cccaaaggcc gttccggcgc
     3721 tcatcgagca gatggacggc ctaggcgagc cctatcggat cctcatcgtc gagaacgggt
     3781 cgaccgacgg gaccgccgcg gcggccgcac aggcggcggc ccacagcccg gtcacggtga
     3841 tgtccctcga cgtcgccgac tacggcgcgg cgatgcgcca cggattcctg gcggccgacg
     3901 gaagctgggt ggtcaatttc gacatcgact acttctcggc ggacttcctg aagagagtgc
     3961 tggcgctcga tgacgtcgat ctcgtcatcg cttcgaagcg ggatcccggc tcggaggaca
     4021 ggaggccact cctcagacgt ttcgctacct gggtgttcaa tctgcttctg cgctcgatcc
     4081 tcgactcgag agtgagcgac acccatggca tgaaggcctt tcgccatgat ttggtctcga
     4141 ccctggcccc gtccgtgcgc agtcggcagg atctgttcga cacggagttg gtgatccgag
     4201 cggagcgggc cggctatcgc atcgaggagg tgccggtggt ggtcgaggag atgagagtgg
     4261 cgcggtcgtc gctggtgaaa cgcgtgcctc ggacgatcag gggtctgctt cgcatccgca
     4321 ggacgttggc cgatgaggcg ttagacctgg cgccggcgca caccccagat tccgacgccg
     4381 attaggccga gcacgccgat gagggtggca atcttcccac ccgtctccgc ccatgtgtcc
     4441 tggaattcga ggacgacctg ctcgctggtg gggaccacga ccatcaacga cggagtagcc
     4501 cgccaaggcc cgtctgcacc ggtggcggtc cagttgggga aataggagac cttgataagg
     4561 tggggcaatc ccaccgcggt ggtgttgaag gagatcctgt ggttttcgac gacgatgtca
     4621 ctcacggcgt cggccggtgc gtccagagcc acgtcgggtc tctgctcgat cgactcgata
     4681 cgaggccact ctgcggggcc gtcggtcact accaatctgt cgagattgtc cacgtcctcg
     4741 taccaggtga gcgccatgtc gaagaaactg ggcagctcct caccatccga cccggtgacg
     4801 gtgtcggaac cggtgagcga gtcgagcagt ccatgctccg ggacctcgta taccgacgga
     4861 aggaatgagg ccacctcgac cagctccgtc tcggggagcc ggtagatgct gaagggcccg
     4921 gtcgtggcga gcttcgacat ctcggggatc ccgtcggcac gttcggtggc ctcgggcgtc
     4981 accgacacgt agtagtcgac gccgaacagc tccagatgct tgagcccacg ctccatctca
     5041 cccgtgtggt actgcagccc ggggatcggg ttcgacggcc tctccgacat ctccgacgag
     5101 ttgatgaagt ggaacggcgt ggtcagagac gactcgaaga acagcccttc catcgatgga
     5161 tgcgagccct cggtccaata tgggatcagc atcggggaca tgggagtccc gtatacaccg
     5221 agctccttgt tggcggcctc ccagtggacc ctgccctcgg ggagggtgtc gatctcggcc
     5281 atcaacgcct cgtactcggg ccatttcggc ttgttctcgt agcctgcgta gttccacttc
     5341 gcccacccgt cgacaaaggt cacgccggac agagcaccca ggaccaccac gcaaacagtg
     5401 gcaccggtga gaaacgtgcg ggtcgacatg ctggactccc agacgaacgt gagcccgatc
     5461 accagtgccc cggcgatccc gacgggtatc gccgcccacg ccgggaactc agtcgaatc
//
LOCUS       DASTXO010000464         3313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_286232, whole genome shotgun sequence.
ACCESSION   DASTXO010000464 DASTXO010000000
VERSION     DASTXO010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286232"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VFU96_09805"
     CDS             <1..624
                     /locus_tag="VFU96_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5113603.1"
                     /translation="VVDDGSTDGTAAVAEIWSNKLPLQVMTHDQNQGLGATLRDGVEW
                     ACGLAQPSDVIVTLDSDNTHTPELILRMVRMVREGHDVVVASRFVRGSKVRGVPFGRR
                     VLSRVASALFKVVFPIPGIRDYTCGYRAYRAGLLQDVIEKDPGFFDQDGFQVMVDILL
                     KLRRDRDLIFGEVPLILRYDFKEGSSKMDVGGTIRGTLALMWRRRFT"
     gene            621..1280
                     /locus_tag="VFU96_09810"
     CDS             621..1280
                     /locus_tag="VFU96_09810"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5113604.1"
                     /translation="MTKAPISEPAHYFDQVYRDYELQNPERKLDHYLNRIDARTSSGP
                     KDLLDIGCGLGSFLARAAHRHPDWTFFGTDIAAEAISQTEGRLPEGTIVPASAEQTPF
                     AAGSFDIITAWDVFEHVPDLGAVASSVSQMLRPGGLLVFVVPVYDGVTGPVIRRLDKD
                     PTHLHKWSRQDWVDWATSRFGDVEWHGLLRYLVGRTYLHIPTVRARRHTPAILVSCRR
                     P"
     gene            complement(1241..2665)
                     /locus_tag="VFU96_09815"
     CDS             complement(1241..2665)
                     /locus_tag="VFU96_09815"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEU5113605.1"
                     /translation="MAESEPSGGDLTEVISNDGSRMLSRAQLRLLGIALVTLGASHVS
                     TGVSAGLVIVGLVFALAGTFGQFPATNGSEVRWAAATALVMLIGILGPPIGTYPSSIA
                     GIRPGLIVMGGMACVIAFGLGHRYTGRLVAVALVLGVSVVTTGALIAHEWNSAVGTDI
                     YSAHRAAGEALAGGQNPYTDAVRFEDGNPYQEGRVFEGYPYPPVVVGTYGLAASITDP
                     RLISSVAWLFFLGWLGWRALRRRSDEASGFSLAVLLVLGLSPLNSLVWFMAWTEPLTL
                     AFFAVAAILWDRYPKSSGVFLGLALASKQYLVFLAPMLVLHRDRGSKARSIVAAGVAG
                     LTILVGLAPDPPAFLRSTIGNLADIAFRPDTQSLPGLLAALGLEINLPNILWILVGLG
                     VTTLLAKSSSTSSGFMIRAGLGLGTAFVLGMAFPNYWFLVAGLLAIGTVLEPSDTGVL
                     HPVQGRHILDSSQGRRQETRIAGV"
     gene            complement(2658..>3313)
                     /locus_tag="VFU96_09820"
     CDS             complement(2658..>3313)
                     /locus_tag="VFU96_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113606.1"
                     /translation="FLLNPGILVSSVIDTAEVLAMLFVVAAALFLLRERLGVSVVFLT
                     LAALTRETMLLATVGAIVYVWHKKRSVPKVLFVPFVASAAWWLYVRLQLGHLDASVQD
                     TQALGLPFKGFYEAFQIWISEPGSDVDLIVGIVLLVVSVLVAIRAVTDRSLLGLMGAG
                     FVLLAVLMVEGVWRFYFDASRALVPVITIYVLTVSASRQRQKLQPKRIPVPVDQPSG"
BASE COUNT          722 a         1002 c         1032 g          557 t
ORIGIN      
        1 gtcgtggacg atggctcaac tgacgggacc gcagccgtcg ccgagatctg gtccaacaag
       61 ctccccctgc aggtgatgac gcacgatcag aatcaaggct tgggggcgac cttgcgtgat
      121 ggagtcgagt gggcgtgcgg ccttgcccaa ccaagtgacg tcatcgtcac gctcgactcg
      181 gacaacacac atacccccga gctgattctg cgcatggttc ggatggtccg cgagggccac
      241 gacgtggtcg ttgcctctcg atttgtgaga ggcagcaaag tgcggggagt tcctttcggc
      301 cgccgcgtcc tcagccgagt ggccagcgcg ttgttcaagg tcgtgttccc gataccaggc
      361 atccgtgatt acacctgcgg ctatcgcgcc taccgtgccg ggctgctcca ggacgtgatc
      421 gagaaagacc ctgggttctt cgaccaggac ggatttcagg tgatggtcga catcctgctc
      481 aagctccgac gggaccgtga cctcatcttc ggcgaggttc cgctgatcct caggtacgac
      541 ttcaaggaag gttcatccaa gatggatgtc ggaggaacaa ttcgcgggac cctggctctg
      601 atgtggaggc ggaggttcac atgaccaagg cgcccatcag cgagccggcc cattacttcg
      661 accaggtgta tcgggactac gagctccaga atccggagcg caagctggac cactacctca
      721 accggatcga tgctcgcacg agctcgggcc ccaaagatct tctcgacatt ggttgtggtc
      781 tgggctcttt cctggcgcgg gcggcccacc gtcatccgga ttggaccttc ttcggcaccg
      841 acatcgcagc ggaggcgatc tcacagaccg aaggccggct gccagaaggc accatcgtgc
      901 cagcttcggc agagcaaact cctttcgccg ccgggtcgtt cgacatcatc acggcatggg
      961 acgtcttcga gcacgtgccg gacctcggtg ccgttgcctc gtcggtatcc cagatgctca
     1021 ggccgggggg ccttctcgtt ttcgtcgtgc cggtctatga cggcgtgacc ggcccggtga
     1081 ttcggcgtct cgacaaagac ccgacacatc tccacaagtg gtcaaggcaa gactgggtcg
     1141 actgggcgac tagccgattc ggcgatgtcg aatggcatgg gttgctcaga tatctcgtgg
     1201 ggcggacgta tctccacatc ccgaccgtcc gagcacgacg tcacacgccg gcgattctcg
     1261 tttcctgtcg ccgtccctga gaggaatcga ggatgtggcg gccttggacg ggatgcaaca
     1321 cgccagtgtc cgagggctcg agcaccgtgc cgatcgccag aaggccggca accaagaacc
     1381 agtagttggg gaacgccatc ccgagcacga aggcagtacc gaggccgagc ccagcccgaa
     1441 tcatgaagcc tgacgaagtc gatgaagact tggccaaaag ggtggtaacc ccaagcccta
     1501 cgagaatcca aaggatgttg ggcaggttga tttcaagccc cagagcggcc aacaagccgg
     1561 ggaggctctg cgtgtcgggt cgaaaagcga tatcggcgag attgccgata gtggagcgca
     1621 gaaatgcagg ggggtcaggg gctaatccca cgagaatcgt caacccggca acgccggcgg
     1681 ccactattga cctggccttg gacccgcggt cacgatggag cacaagcatc ggtgccagaa
     1741 acaccaagta ctgctttgac gcgagggcca atcccaagaa gacgccggac gatttcggat
     1801 agcgatccca caagatggcg gcgaccgcga aaaaggcgag cgtgaggggc tcagtccacg
     1861 ccatgaacca gaccagactg ttcaacggcg acagtcccag gaccaaaagg accgccaagc
     1921 taaaacccga tgcctcatcg gaccgccggc gaagggcacg ccagcccagc cagccaagga
     1981 agaagagcca agcgacggaa ctgatcagcc gcgggtctgt gatcgacgcc gccaatccat
     2041 aagtgccgac caccaccggc ggatacgggt aaccctcgaa cacccgaccc tcctggtacg
     2101 ggttgccatc ctcgaaccgg accgcatcgg tgtaagggtt ctgtccgccg gcgagtgcct
     2161 cacctgctgc tcgatgagcg ctgtagatgt cggtcccgac ggctgaattc cactcgtgag
     2221 ctatcaaggc gcctgtcgtg acgacggaga ctcccaggac gagtgcgacg gcaacgaggc
     2281 gaccggtgta gcgatggccg aggccgaagg cgatgacaca cgccatccct cccatgacga
     2341 tcaagccggg gcggatgcct gcgatcgatg acgggtaggt gcctattggg ggaccgagga
     2401 tgcctatgag catgactagg gcagtcgccg cggcccagcg cacttcggaa ccgttggtcg
     2461 ctggaaattg cccgaaagtg ccggccaaag cgaagaccaa tcccacgatg accaggccgg
     2521 cactgacacc ggtcgatacg tggctagctc cgagggtgac cagggcgatt ccgagcagac
     2581 gcagttgagc cctcgacagc atcctggagc cgtcgttcga tatcacctcg gtgagatcgc
     2641 ccccacttgg ctcgctttca gccactgggc tgatcgacag gtaccgggat tcgcttcggt
     2701 tggagcttct gtcgctgacg tgacgctgac accgtcagga cgtagatcgt gatcaccggg
     2761 acgagggcgc gcgaggcgtc gaaatagaat cgccacactc cctcgaccat gagcacagca
     2821 aggagtacga agccggctcc catcaagccg agcaggcttc ggtcggtgac tgctcggatc
     2881 gccaccagga ccgaaactac caacaggacg atcccgacga tcaggtcgac gtctgagcct
     2941 ggctccgaga tccagatctg gaaggcttcg tagaagcctt tgaatggaag cccgagcgcc
     3001 tgggtgtcct ggacactcgc gtcgagatgt ccgagctgca atcgaacata cagccaccaa
     3061 gcggcgctgg ccacgaatgg cacgaacaag accttcggaa cggagcgttt cttgtgccag
     3121 acgtacacga tggcaccaac tgtcgccagg agcatcgttt cccgggtaag agcagcgagt
     3181 gtcaggaaga cgacagagac accaagtctt tcccggagga ggaacaatgc ggcagccact
     3241 acgaagagca tggcgagcac ctcagcagtg tcgatcacac tcgagacgag gatcccgggg
     3301 ttgagcagga atg
//
LOCUS       DASTXO010000465         2813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_469781, whole genome shotgun sequence.
ACCESSION   DASTXO010000465 DASTXO010000000
VERSION     DASTXO010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469781"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..674
                     /locus_tag="VFU96_09825"
     CDS             132..674
                     /locus_tag="VFU96_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5113607.1"
                     /translation="MWLLLNNDTDFFDGRAKKVLHFAPERCLEPLLRQRLGAGYRTAD
                     LAREDVDLRLDVTDIQFPDESFDVIICSHVLEHVPDDGRAMEELHRILASDGWALVVV
                     PVTKDKTFEDPTIVDPDERLRVFGQRDHVRRYGPDIADRLRRAGFVVATHRASDAHTV
                     SDISRMGLRPASRPVFTVTK"
     gene            complement(693..>2813)
                     /gene="topA"
                     /locus_tag="VFU96_09830"
     CDS             complement(693..>2813)
                     /gene="topA"
                     /locus_tag="VFU96_09830"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEU5113608.1"
                     /translation="QEARRILDRLYGYEVSPVLWRKVQQGLSAGRVQSVAVRIVVERE
                     RERMAFTSAEYWSVEGTFAPLDDLKRTFEATLLDVDGSRVATGKEFGADGQPKPGTLV
                     LDQTLATELAQGLLDSRFEVRSVEEKPYKRSPYPPFRTSTLQQEASRKLRFSSSRTMS
                     VAQRLYENGYITYMRTDSTSLADSAVASARAQITKLYGQDYLSPKPRTFATKVKGAQE
                     AHEAIRPAGDEQFQTPEQVATLVGPDEGRLYDLIWKRTIASQMADAVGFTVSARLGAQ
                     STDGREAVFSTSGRTITFPGFLRVYVQGSDDPESDPDDKEKALPKLTTGDALAVQRLE
                     PTGHETRPPARYTEASLVQRLEELGVGRPSTYASIMTTIQDRGYVWKKGTALVPSFVA
                     FGVVGLLEKHFPQLVDYSFTASMEDDLDRIAAGEEESVPWLTHFYFGDDGLKHKVATR
                     LDEIDPRAVGSIPIGVDDGGNDVVARIGRYGPYVEIGEERASIPDDLAPDELTVEKAL
                     ELVLTQNAGDKIFGTDPESGLTVYGKTGRFGPYIQLGEPEEGNKTKPKRASLFKTMSM
                     DELSLDMALQLLALPRNVGTDPDGVEIVALNGRYGPYIQRGSDRRSLESEDQLFTVTV
                     DEALRLLAEPPRRRGQQAAGPLKELGPDPVSGKPVTVRNGRYGPYVTDGDVNASLRQG
                     DSPETIDIERAAELLAARRDRVGK"
BASE COUNT          448 a          902 c          908 g          555 t
ORIGIN      
        1 tcccctttcg cgggataggg aggtggtgcc cggtttgcgg gcgatcatcc cgacggttcc
       61 gtccttacgg gaatccgccg cgtgccgaag cccaatgcat ccactgtgga tcgctggaac
      121 gccaccgatt cgtgtggctt ctcttgaaca acgacaccga tttcttcgac ggccgcgcca
      181 agaaggtcct gcattttgca cccgagcgct gcctcgagcc tctcctccgc cagcgtctgg
      241 gtgccggcta tcgaaccgcc gatctcgcac gtgaagacgt tgatctgcga ctggatgtga
      301 ccgacatcca gttcccggac gagtcgttcg acgtcatcat ctgcagccat gtcctcgagc
      361 atgtgcctga cgacggacgg gcgatggaag agctgcaccg catcctcgcc tccgatggct
      421 gggcgctggt cgtcgttccg gtgacgaagg acaagacgtt cgaagatccg acgattgtcg
      481 atcccgatga gagacttcgt gtcttcgggc agcgagatca tgtgagaagg tacggccctg
      541 atatcgccga tcgtcttcgg agggccggat tcgttgtcgc cacccaccga gcctccgatg
      601 ctcacactgt gagcgacatc tcgcgcatgg gattgcggcc ggcctctcgg cccgtgttca
      661 ccgtgaccaa atgaggcgac ggtaggtcgt cgctacttcc ccacccggtc ccgccgtgcg
      721 gcgaggagct ccgcggctcg ctcgatgtcg atggtctccg gcgagtcgcc ctggcgcagc
      781 gaggcgttga catcgccgtc ggtcacgtac gggccgtatc gcccgtttct gaccgtcacc
      841 ggcttcccgc tgaccgggtc ggggcctagc tccttcagcg gcccggccgc ctgctgaccc
      901 cttcggcgcg gcggctcggc caggagccgc agcgcctcgt cgacggtgac tgtgaagagc
      961 tggtcttcac tctcgaggct gcgccggtcg gaccctcgct ggatgtacgg cccgtagcgc
     1021 ccattgaggg cgacgatctc gacgccgtcg ggatcggtgc cgacgttccg gggtagagct
     1081 agcagctgta aagccatgtc cagcgagagc tcgtccatgg acatcgtctt gaagagggag
     1141 gcccgcttgg gcttggtctt gtttccctcc tcgggctcgc cgagctggat gtagggaccg
     1201 aatcgaccgg tcttgccgta tacggtcagc ccggattccg ggtcggttcc gaagatcttg
     1261 tcgcccgcgt tctgagtcag cacgagctcg agagcctttt ccaccgtcag ctcgtccggc
     1321 gccagatcat cggggatgga ggctcgctcc tcgccgatct ccacgtaagg cccgtagcgc
     1381 ccgatcctgg ccacgacgtc gttgcccccg tcgtcgacac cgatcgggat cgagcccacg
     1441 gctcgggggt cgatctcatc gagacgggtg gccactttgt gcttcagccc gtcgtcgccg
     1501 aagtagaaat gagtgagcca cgggacggat tcctcctccc cggcggcaat cctgtcgaga
     1561 tcgtcttcca tcgatgccgt gaagctgtag tcgacgagct gggggaaatg cttctcgaga
     1621 agcccgacga cgccaaacgc gacgaagctc gggaccaggg ccgtgccctt cttccacaca
     1681 tagccgcggt cctggatcgt ggtcatgatc gaggcgtatg tcgatggccg ccccacgccc
     1741 agctcctcga ggcgctggac gagcgaggcc tcggtatacc tggccggcgg cctggtctcg
     1801 tgcccggtgg gctccagacg ctggacggcg agagcgtcac ctgtggtcag tttgggcaga
     1861 gccttctcct tgtcgtctgg gtctgactcc gggtcgtctg atccctgcac gtagacgcgg
     1921 aggaatccgg gaaaggtgat cgttcggccg ctggtgctga acacggcctc gcgcccatcg
     1981 gtcgactggg ccccgaggcg agcggacaca gtgaagccca ctgcatccgc catttgcgat
     2041 gcgatggtcc gcttccagat gagatcgtag agccggccct catcggggcc gacgagggtt
     2101 gccacctgtt cgggtgtctg gaattgctcg tctccggccg gccggatcgc ttcgtgtgcc
     2161 tcctgggctc ccttcacttt ggtggcgaag gtccgcggct tgggcgagag atagtcctgc
     2221 ccgtagagct tggtgatttg ggcccgtgcc gaggcgactg cggagtcggc caacgaggtc
     2281 gagtcggtcc gcatgtaggt gatgtacccg ttctcataga gacgctgggc gaccgacatc
     2341 gttcgtgacg acgaaaaacg gagctttcgg gaggcctcct gctgaagagt cgacgtccga
     2401 aatggcggat acggcgaccg cttgtagggc ttctcctcga ccgaccgcac ctcgaatcga
     2461 gagtcgagga gaccttgggc cagttctgtc gccagggtct ggtccaacac gagagttccc
     2521 ggcttgggct gaccgtcggc accgaactct tttccagtgg ccaccctgga cccgtccaca
     2581 tcgaggagcg tggcttcaaa ggtcctcttc aggtcgtcta ggggggcaaa agtgccttcg
     2641 acgctccagt attcggccga ggtgaacgcc atccgctcac gttcgcgctc gacgacgatg
     2701 cggacggcga cactctgcac ccgcccggca gacaggccct gctggacctt gcgccacagg
     2761 accggggaca cctcatatcc gtagagccgg tcgaggatgc ggcgtgcttc ctg
//
LOCUS       DASTXO010000466         2517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_691581, whole genome shotgun sequence.
ACCESSION   DASTXO010000466 DASTXO010000000
VERSION     DASTXO010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691581"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VFU96_09835"
     CDS             <1..594
                     /locus_tag="VFU96_09835"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEU5113609.1"
                     /translation="ATPDMVDVDVGVSVLANTVDDASRTAAAKANAVASALTAAGIAA
                     DDLTTTEYSIHPEYDYSDSKQRLLGYRVSNTVRAKLRDITSTGPLLDSIASAGGDETR
                     VNGMSFGVADETSLLGRAREAAWNDARAKAEQLAALSGHTLGKATTITEVVQSPVVPM
                     PRMMAADMAMSERASTPIQPGTSSVTVTLQVEFGFRE"
     gene            656..1993
                     /locus_tag="VFU96_09840"
     CDS             656..1993
                     /locus_tag="VFU96_09840"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU5113610.1"
                     /translation="MNDHSRRIYDSMLGLLSNAENPTPLVRLNHVVPFRHTEVYAKLE
                     WYNPFGSVKDRIASNMIQQARDQGELTDEDLVEPTSGNTGLGLIMMGNLLGYDLTTPL
                     SSAIPVEKRTMLRFFGATVEELEDDLCPAPWAPEGAIARATEIAEKPDFHMLNQYRNP
                     SNPDAHYRTTGPEIWRQTDGAITHFVAGMGTCGTITGTGRFLKEQSSGVQVIGVHPGE
                     GHDIPGVRSIRQLKQTEFFLPDEYDGMIEIGNEDAYALTLRLNREESIPAGPSSGMAL
                     AGALATVPDRPGVKVVVMFPDNAFKYASSMAKHIPGLAPTALPRLKSRREELFDAIIE
                     HTRHNPHLTVDVEAAHDKWEAERPVVVDVRSAEEFAQGHIPGAVSIPLLELPDRQDQL
                     PTDLDTPVLTVCARGNISLPAVLYINSLGYRDAHSITGGTNAWVEQGYEVESA"
     gene            2039..>2517
                     /locus_tag="VFU96_09845"
     CDS             2039..>2517
                     /locus_tag="VFU96_09845"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464799.1"
                     /note="catalyzes the conversion of N-acetylornithine to
                     N-acetylcitrulline in an alternative arginine biosynthesis
                     pathway; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine carbamoyltransferase"
                     /protein_id="HEU5113611.1"
                     /translation="MRHFLNTFDWTRPELQSMVDEGRRLKASPYGKTLEGKTVALLFL
                     NNSLRTRTSFDVGANQLGGHAVVLSPSGGMWPLEFEEGAVMLDDAEEHVKEAAKVLSR
                     YCDLIGIRAFPKFENWEEDRKDTILQAFATHSDVPVINMETITHPCQELAHVMALQER
                     "
BASE COUNT          507 a          853 c          758 g          399 t
ORIGIN      
        1 gcgaccccgg acatggtcga tgtggatgtc ggggtctctg tcctcgccaa cacagtggac
       61 gacgcctcgc ggaccgcggc agcgaaggcg aacgcggtgg cgtcagcgct caccgcggcc
      121 ggcatcgccg ccgatgacct gacgacgacg gaatactcga tccaccccga gtacgactac
      181 tcggacagca aacagcgact gttgggttat cgggtcagca acaccgtccg tgccaagctc
      241 cgtgacatca cttcgaccgg cccacttctc gattccatcg cttcggccgg tggcgatgag
      301 acccgggtca acggcatgag cttcggagtg gccgacgaga catctctcct ggggcgcgct
      361 cgtgaagcgg catggaacga tgccagagcc aaagcagagc agctggcggc cctctccggg
      421 cacacgctgg gcaaggccac caccatcacc gaggtggttc agagccctgt ggtgccgatg
      481 ccgaggatga tggcggcaga catggcgatg tcggagaggg cgtcgacgcc gatccagccc
      541 ggcacgtcat cggtgacggt gacgctgcag gtggagttcg gcttccggga gtgaactgcc
      601 cggggggaat acccgccgcc ttcctatcat tgagattggc aacaggcgag cgcaattgaa
      661 cgatcactca cgccgcatct acgactcgat gctcgggctg ctctcgaacg ccgagaaccc
      721 gacccccctg gtgaggctca accacgtcgt cccgttccga cacaccgagg tctacgccaa
      781 gctcgagtgg tacaacccgt tcggctcggt caaagaccgc attgcgtcca acatgatcca
      841 gcaggcgcgg gaccaaggcg agttgaccga tgaagacctg gtcgagccaa cctccggcaa
      901 cacaggtctc ggcctgatca tgatgggcaa cctcctcggt tatgacctga ccactccgct
      961 gtcgagcgca atccccgtcg agaagcggac gatgcttcgg tttttcggag ccaccgtcga
     1021 ggaattggag gacgacctct gcccggcgcc gtgggcaccc gagggcgcca ttgctcgtgc
     1081 gacggagata gccgagaagc ccgatttcca catgctcaac caataccgca acccgtccaa
     1141 cccggacgcc cactatcgca ccacgggacc ggagatatgg cgccagaccg atggggcgat
     1201 cacacatttc gtggcgggga tgggtacatg cggcaccatc accggaaccg ggagattcct
     1261 gaaagagcag agctcaggtg tccaggtcat cggtgtccac ccgggtgagg gacacgacat
     1321 ccccggcgtc cgcagcatcc gccagctcaa gcagaccgag ttcttcctcc ccgacgagta
     1381 cgacgggatg atcgagatcg gcaacgaaga cgcgtatgcc ctgacgctgc ggctcaaccg
     1441 agaggagagc atccccgccg ggcccagctc ggggatggca ctggccggcg ccctggccac
     1501 cgtccccgac cgacccgggg tgaaagtggt cgtgatgttc ccggacaacg ccttcaaata
     1561 cgcctcgtcg atggcaaagc acatccccgg cctggcaccg actgcgctgc cccgactcaa
     1621 gagccgccgc gaggagctct tcgacgccat catcgagcac accaggcaca acccacatct
     1681 caccgtcgat gtcgaggcgg cacacgacaa atgggaagca gagcggccag tcgtagtcga
     1741 tgtccgctcc gccgaggagt tcgcccaggg ccatatcccg ggcgccgtca gcatccccct
     1801 cctcgagctg cccgatcgac aggaccagct gcccaccgac ctcgacaccc ccgtcctgac
     1861 ggtctgcgca cggggcaaca tctcgctccc ggctgtcctc tacatcaact cgctcggcta
     1921 tcgagacgcc cacagcatca ctgggggcac caacgcctgg gtggagcagg gctacgaggt
     1981 cgagtccgcc tagtcctgct cctttggatt tcggggtctg ccttatcaaa ctgcggcgat
     2041 gcggcatttc ctcaacacct tcgattggac ccgacccgag ctccagtcga tggtggacga
     2101 agggcgccga ctgaaggcat cgccgtatgg gaagacgcta gagggaaaga ccgtcgccct
     2161 gctcttcctc aacaactccc tgcgcacccg cacctcgttc gacgtcggcg ccaaccagct
     2221 gggaggccac gccgtcgtgc tctccccctc cggtgggatg tggccgctcg agttcgaaga
     2281 aggggcggtg atgctcgatg acgccgagga gcacgtcaaa gaggcggcca aagtcctctc
     2341 ccgctactgc gatctgatcg ggatcagggc ctttcccaaa ttcgagaact gggaggagga
     2401 ccgcaaggac accatcctcc aggccttcgc cacccactcc gacgtccccg tgatcaacat
     2461 ggagacaatc acccatccgt gtcaggagct ggcgcacgtc atggcccttc aagagcg
//
LOCUS       DASTXO010000467         3805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1207099, whole genome shotgun sequence.
ACCESSION   DASTXO010000467 DASTXO010000000
VERSION     DASTXO010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207099"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VFU96_09850"
     CDS             complement(<1..621)
                     /locus_tag="VFU96_09850"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5113612.1"
                     /translation="MNDDLRAKVAADRPRLTELLGDLVRLPTVSAPGYDQVKVREAAE
                     AIVAILDREGFENAQLLGLEAGNPAVFAEIPAPVGAPTLLLYAHYDVQPPGPAHEWST
                     DPFEPVEREGRLYGRGAADDKGGIVMHLGAIAAHDGRPPVGVQIFFEGEEEAGSQSLA
                     GILDRYSSLLHPDVIVIGDGGNWSVGVPAFITSLRGIVGVSFELRTL"
     gene            644..1462
                     /locus_tag="VFU96_09855"
     CDS             644..1462
                     /locus_tag="VFU96_09855"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU5113613.1"
                     /translation="MSPRLGGWAIMGAMPSFRLFGIPVTIRPSFLVIAALIGFTSRRP
                     IVYLVAWVAIVFLSILIHELGHAITARSYGAEVAIELNGIGGLTSWSVPDRELGPGQR
                     ALVAAAGSGVGVLFGCAVWLFERFTGPYDEVAGFVVSNLILVNVFWGLLNWLPIRPLD
                     GGHLFTSLLQKVIPSRAELIANVVFFITAAAALVVALLTQRIFLALIAGWLLMGELTR
                     GRGGQRRPPTPIPQMTFDPPQGEDDATVAKESVLEQDHESQPNPEPETRAEPDN"
     gene            1486..2724
                     /locus_tag="VFU96_09860"
     CDS             1486..2724
                     /locus_tag="VFU96_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018959574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5113614.1"
                     /translation="MEFAYSPRVEELRARLLAFMDDLVYPAEARYEEELAASGDPHHH
                     PAVIEELKTEARKRGLWNLFLPDPELGAGLSVLEYAPLAEITGRSPEIAPEVINCSAP
                     DTGNMEILHLFGTAEQKETWLAPLLDGEIRSCFSMTEPDVASSDATNIRTRIVRDGEE
                     YRITGRKWFSSGAASSRCKFSIVMGVTDADAEPHRRQSMVLVPMDSPGVEVERQPTVF
                     GYQDRGGHPQIRFDDVRVPVTNLLGEQGGGFAIAQARLGPGRIHHCMRAIGAAERCLD
                     LMVQRSQARVAFGQPLSEQGVIQEWIADARIRIEQARLLVLKTAWMIDEVGVKAARTE
                     IAAIKVVSPALLTEIADRAIQLFGAAGFTGDFPLARFYSMGRWLQIADGPDEVHRRSV
                     ARAELGRLGPGAAPEPYPFD"
     gene            2829..3107
                     /locus_tag="VFU96_09865"
     CDS             2829..3107
                     /locus_tag="VFU96_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005964615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEU5113615.1"
                     /translation="MVGPVTFDVRFSTWRDDAACLDIVDDVSFFPDAEDLSAIARAKA
                     VCATCPVASECLTWAIETNQSEGVWGGHTPKERRSIRRRWIEEIEQAS"
     gene            complement(3146..>3805)
                     /locus_tag="VFU96_09870"
     CDS             complement(3146..>3805)
                     /locus_tag="VFU96_09870"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HEU5113616.1"
                     /translation="RVFPGSPRGSTASRLAHLFMNEVVSHCSVGIDCHTASNHRTNLP
                     QLRADIDDPDTLDLARAFGAPFTIKARVRDGSLRQAATEQGIKMLLYEAGQADRTDED
                     AIALGFEGVMRTLRSMGMIEMRLPRPRPTTLVRRTRWMRARRGGLVEISPKLGDRVEE
                     GDEVARISDAFGDRPTRVKTTTSGWVIARTLNPLVNPGDPLLHVAAEHGPDVDEPAER
                     R"
BASE COUNT          636 a         1234 c         1238 g          697 t
ORIGIN      
        1 cagggtgcgt aactcgaacg acacaccgac gatgccccgc agcgaagtga tgaaagcggg
       61 cacgccgacc gaccaattcc ccccgtcacc gatgacgatg acgtcggggt ggaggaggct
      121 cgagtagcga tcgaggatgc cggcgaggct ctgagatccg gcctcctctt caccctcgaa
      181 gaagatctgg acgccaactg gcggccggcc atcgtgggcg gcaatggcac cgaggtgcat
      241 gacgatccca cctttgtcgt cggccgcacc ccgtccatac agccgtccct cgcgctcgac
      301 gggctcgaaa ggatcggtgg accactcgtg tgcgggaccg ggcggctgca cgtcatagtg
      361 ggcgtagaga agaagcgtcg gggcacccac gggggcgggg atctccgcaa aaaccgccgg
      421 gttgcctgcc tccaacccca ggagttgggc gttctcgaat ccctcccggt cgaggatcgc
      481 gacgattgcc tccgccgcct cccgcacctt cacctggtcg tagccggggg cgctcacggt
      541 cggaagcctg accagatcgc cgaggagctc ggtgagtcga gggcggtcgg cggcaacctt
      601 ggctcgtaga tcgtcgttca tcggccgagc gtaactgggc cccttgtccc caagactcgg
      661 cgggtgggcc atcatggggg cgatgccctc cttccgtctg ttcgggatcc cggtgacgat
      721 ccgcccgtcg ttcctggtga tcgccgccct catcgggttt accagccgga gaccgatcgt
      781 ctacctggtg gcctgggtgg cgatcgtctt cctctcgatc ctcattcatg agctggggca
      841 cgcgatcacg gcccgctcct atggggccga ggttgccatc gaactaaatg gcatcggggg
      901 tctgaccagc tggtcggtgc ccgacaggga actcggcccg gggcagcggg ccttggttgc
      961 cgctgccggc tccggggtgg gggtgctctt tggttgcgcg gtctggttgt tcgagcgctt
     1021 caccgggccg tatgacgaag tcgccggatt cgtcgtctcc aatctcatcc tggtcaatgt
     1081 cttctgggga ctgctcaatt ggctaccgat ccgacccctc gacggcggcc atctcttcac
     1141 ctccctgctg cagaaggtga ttccctcgcg ggccgagctc attgccaatg tcgtgttctt
     1201 catcaccgcc gccgcggctt tggtcgtggc cctgttgacc caacggatct tcctcgccct
     1261 catcgccggg tggctgctga tgggggagct gacccgaggc agaggtggcc agagacggcc
     1321 tcccacgccc atcccccaga tgacctttga tcccccacag ggcgaggacg acgccacggt
     1381 cgcaaaggag tcggtgctcg agcaagatca cgagtcgcag ccaaatccgg agcccgagac
     1441 ccgggccgag cctgacaact agaaggcaag tagcttcccg ccacgatgga gttcgcctac
     1501 agcccccggg tcgaggagct gcgggctcgt ctgctggcat tcatggacga tttggtgtac
     1561 ccggcggagg cccggtacga ggaggagctg gcggcctccg gagaccctca tcatcaccct
     1621 gcggtgatcg aagagctcaa gaccgaggcc cgcaaacgcg ggctgtggaa cctcttcctc
     1681 cctgatcccg agctcggcgc cggactctcg gtcctcgagt acgccccgct tgctgagatc
     1741 accggacgca gccccgagat cgcacccgag gtgatcaact gctcggcgcc cgacaccggg
     1801 aacatggaga tcctccacct cttcggtacg gccgagcaga aggagacctg gctggcgcca
     1861 ttgctggatg gtgagatccg atcctgcttc tcgatgaccg agcccgacgt cgccagctcg
     1921 gacgccacca acatccgcac tcgcatcgtt cgcgatggtg aggaatatcg gatcaccggc
     1981 cggaagtggt tcagttcggg ggccgcctcg agtcggtgca agttctcgat cgtcatgggg
     2041 gtcaccgacg cggacgccga gccgcaccgt cgccagtcga tggtgctggt ccccatggac
     2101 tcccctggtg tagaggtgga gcgtcagccg actgtgttcg gctatcagga tcggggcggt
     2161 cacccgcaga tccgcttcga cgacgtgaga gtcccggtca ccaatctcct cggcgagcag
     2221 ggcggcgggt tcgccatcgc ccaggcccgg ctcggccccg gccgaattca tcattgcatg
     2281 cgggcgatcg gcgccgccga gcgctgcctc gacttgatgg tgcagcggtc tcaggcccgg
     2341 gtcgccttcg ggcagccact gtcggaacag ggggtcatcc aggaatggat cgctgacgcc
     2401 aggatccgca tcgaacaggc ccgtctcctg gtcctcaaga cggcctggat gatcgacgaa
     2461 gtgggtgtga aggcggcgcg caccgagatc gccgcgatca aagtggtgtc gcctgccctt
     2521 ctcaccgaga tcgccgatcg cgccatacag ctctttgggg ctgccggctt cacaggggac
     2581 ttcccattgg cgcgcttcta ttccatgggg cgctggctcc agatcgccga tgggcccgac
     2641 gaagttcatc gtcgatcagt ggctcgagca gagctgggcc gcctcggccc gggggcggcc
     2701 cccgagccct atccgttcga ctgactccaa cgcccaatcc aaagtctctg ccggctccga
     2761 acagtgtttg acatgacgtc ccgaatggga acgaatgagc ttgacattgc gtttacggga
     2821 gttcagagat ggttggccct gtgaccttcg atgtgaggtt ctcgacttgg cgggatgatg
     2881 cggcgtgtct cgacatcgtc gatgacgtgt cgttcttccc cgacgccgaa gacctgtccg
     2941 ccatcgctcg ggcaaaggcg gtctgtgcga cctgcccggt ggcgtcggag tgcctcacct
     3001 gggcgatcga gaccaaccag agcgaaggag tgtggggagg tcacaccccc aaggagcgtc
     3061 gctcgatccg ccggcgttgg atcgaggaga tcgagcaagc cagctgaatc gcccggcatc
     3121 gaggggaggt ttccgccctg ccttcctacc ttctctccgc aggttcatcg acgtcagggc
     3181 cgtgctcggc ggcgacgtga agtagcggat cacccgggtt gaccagtggg ttgagggtgc
     3241 gggcgatcac ccaacccgat gtcgtcgtct tgacccgtgt tggtcgatcc ccgaacgcat
     3301 ccgagattcg ggcgacctca tcgccttcct cgacgcggtc tcccagcttc ggcgagatct
     3361 cgaccagacc gccacgccgc gctctcatcc atcgagtccg tcgcaccaga gtggtgggac
     3421 gcggcctcgg caacctcatt tcgatcatcc ccatcgagcg aagcgtccgc atcactcctt
     3481 cgaagccgag cgcgatcgca tcctcatctg ttcggtccgc ttgacccgcc tcatagagga
     3541 gcatcttgat gccctgttcc gtggcggcct gccgaagcga gccgtcccgg acacgggcct
     3601 tgatggtgaa aggggccccg aatgcccgag cgagatcgag agtgtctggg tcgtcgatgt
     3661 cggcgcgaag ttggggaaga ttggtccggt ggttcgaggc ggtgtggcag tcgatcccga
     3721 ccgagcagtg agagaccacc tcattcatga aaaggtgggc gagacgcgat gccgtcgacc
     3781 cgcggggtga gccagggaag actcg
//
LOCUS       DASTXO010000468         2282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1390901, whole genome shotgun sequence.
ACCESSION   DASTXO010000468 DASTXO010000000
VERSION     DASTXO010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390901"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..1112
                     /locus_tag="VFU96_09875"
     CDS             108..1112
                     /locus_tag="VFU96_09875"
                     /inference="COORDINATES: protein motif:HMM:NF026760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-hydrolase N-terminal
                     domain-containing protein"
                     /protein_id="HEU5113617.1"
                     /translation="MATDSSLITWLLKLANRLNPLTSGPEAPLVTGAALDSFGPSLMP
                     RSSMHQGVAGGLAVISARSIGLAVDRVVRLVAPETAPLAWRIGARAALAGGGAALGRI
                     SETEDETTARASLRSAGKLVNVAAIGGIIYEAATDVSKKVDSPIVPIMTGLGGFGYAM
                     TRWSKELDTRQGVVAKWTAEDKPANIFASVAIAAAVANGGRALGRGYSSSRRSMVGYM
                     GSGVGHAAVGRAMNLALWGVGAVALYQGLVARISRSNSKIEPAFAAPPSNGHVSGGPN
                     SISPYSQMGLQGRRYVSEVVTPEVIEQTMGQPAVAHPIRAYVGVEMSRCTPPGAPSSR
                     "
     gene            1079..2020
                     /locus_tag="VFU96_09880"
     CDS             1079..2020
                     /locus_tag="VFU96_09880"
                     /inference="COORDINATES: protein motif:HMM:NF021588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-hydrolase family protein"
                     /protein_id="HEU5113618.1"
                     /translation="MYATGRSELALTEMERLGAFDRSYLLLVSPTGTGWVDHTMIETA
                     EILTRGDIATACIQYGKAPSFLEVQGVSLGRAQFRQLLWGVKQRLRAVPEEKRPKVLV
                     FGESLGAWSSSDVVMHQGIAGFDHYGIHRALWFGLPGLARWSKTGMREGRNRLTPEGT
                     VAPFDNFEQYQALSKEEREKMRAVIVDHDNDPIAQMSLRMAVKRPPWLNDRRGRGVPE
                     SMKWTPIITFTQVLVDAMNAMVTIPGEYKSFGHDYRADTAAFVQAGYGLPEVTDKQMT
                     AIHETLVRRELDRAARIKAGQDAAGQDTMGRVSSDTQ"
     gene            complement(2025..>2282)
                     /locus_tag="VFU96_09885"
     CDS             complement(2025..>2282)
                     /locus_tag="VFU96_09885"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEU5113619.1"
                     /translation="VLVILATGTTFYTLVEGWTVIEALYFTVITLTTIGYGDLHPTTE
                     FSRLFTIFFVLAGVSTLLGFLNFILGRTVKEQVERRQQKGK"
BASE COUNT          415 a          718 c          755 g          394 t
ORIGIN      
        1 caatgacatc gggcgctcca cctggatgcg catctgcagg ggtgtgatcg acgaggcgat
       61 cgcgatcgac gacccggccc tgggcgatcg gtatagcgtc tagggccatg gccaccgact
      121 catcactcat cacctggctc ctgaagctcg ccaatcgtct caatccgctg accagcgggc
      181 cggaagcgcc cctcgtgacg ggggcagccc tcgattcctt cggtccgtca ctcatgcctc
      241 gctcgtcgat gcaccagggc gttgccgggg gcttggcggt gatatcggcc cgctcgattg
      301 gcctggctgt agaccgagtg gttcgactcg ttgctccgga gacggcacct ctggcttggc
      361 ggatcggggc tcgggccgcg ctcgccgggg gcggtgccgc gcttgggcgg atatccgaga
      421 cggaagacga gacgacggct cgagcctccc tccgcagtgc cgggaagctg gtcaacgtcg
      481 cggcgatcgg cgggatcatc tacgaggccg ccaccgacgt cagcaagaag gtcgactctc
      541 cgatcgtgcc gatcatgacc ggtctcggtg gtttcggata tgcgatgacc agatggtcca
      601 aggagttgga cacccgccag ggagtggtgg cgaaatggac cgcggaggac aagccggcca
      661 acatcttcgc ctcggtcgcc atagccgcgg cggtggcgaa cggggggcgg gcgctgggtc
      721 gggggtattc ctcctccaga cgctccatgg tgggatacat gggcagcggg gtcgggcacg
      781 cggccgttgg gcgggcgatg aacctcgcgt tgtggggagt cggagccgtg gccctgtatc
      841 aaggattggt cgccaggatc tccaggagca actccaagat cgagcccgcc ttcgccgcac
      901 cacccagcaa cgggcacgtc tccggtgggc ccaacagcat ctccccctac tcgcagatgg
      961 ggctccaggg gcgccggtat gtctccgagg tggtcacccc cgaggtgatc gagcagacga
     1021 tgggtcagcc cgcagtggct cacccgattc gagcctacgt gggagtggaa atgagccggt
     1081 gtacgccacc gggcgctccg agctcgcgtt gaccgaaatg gagcgtctcg gggcattcga
     1141 tcgaagctat ctcctcctcg tctcccccac cggaaccggt tgggtcgacc acaccatgat
     1201 cgagacggcc gagatcctca cccgcggaga catcgccacc gcgtgcatcc agtacgggaa
     1261 ggccccgtct tttctcgaag tgcagggggt atccctggga agggcccagt tcagacagct
     1321 gctctggggt gtgaaacagc ggctcagggc ggtgccggaa gagaagcggc ccaaggtcct
     1381 cgtcttcggt gagagtctcg gggcctggtc gagctccgac gtcgtgatgc accagggcat
     1441 cgctggcttc gatcattacg gcatccatcg cgcactctgg ttcggtcttc cggggctcgc
     1501 ccgttggtcg aagacgggga tgcgcgaagg gcgcaaccgc ttgacccccg agggaaccgt
     1561 cgccccgttc gacaacttcg agcaatatca ggcgctgagc aaggaagagc gggaaaagat
     1621 gcgtgccgtg atcgtcgacc acgacaacga tccaatcgcc cagatgtcac tgcgcatggc
     1681 ggtgaagcgc ccgccgtggc tcaacgatcg tcgtggccgt ggagtgccgg agtcgatgaa
     1741 gtggactccg atcatcacct tcacccaggt cctcgtcgat gcgatgaacg ccatggtgac
     1801 catccccggc gagtacaagt cctttggcca cgactaccgc gccgacaccg ccgcattcgt
     1861 ccaggccgga tacgggctcc ccgaagtcac cgacaagcag atgaccgcca tccacgagac
     1921 cctggtccgc cgcgagttgg atcgggcggc tcggatcaaa gccggtcagg atgctgcggg
     1981 ccaagacacc atgggccggg tctcctccga cactcagtag cggcctattt gcccttctgc
     2041 tgtctgcgct ccacctgttc tttgaccgtg cgcccgagga tgaagttgag gaagccgagc
     2101 agcgtgctca ctccggcgag cacgaagaaa atggtgaaaa gcctcgagaa ctcggtagtg
     2161 gggtggagat cgccataccc gatcgtcgtc agcgtgatca cggtgaagta gagcgcctcg
     2221 atgaccgtcc aaccctctac cagggtgtag aaagtcgtcc ctgtggcgag tatcaccagg
     2281 ac
//
LOCUS       DASTXO010000469         2350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1575108, whole genome shotgun sequence.
ACCESSION   DASTXO010000469 DASTXO010000000
VERSION     DASTXO010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1575108"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="VFU96_09890"
     CDS             complement(<1..789)
                     /locus_tag="VFU96_09890"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5113620.1"
                     /translation="MSQSSAGYAPSGDALIAYESGGEGSAVIFIHAGIADRRMWRHQL
                     SEIPPGFRFVSLDLRGHGSTELTDARFSNHEDVLAVMDHFDIDEAVVVGCSMGGGTTF
                     DVALASPDRVSGLVVIGAGAPGFDVDDYQPPQWLEVLKAYEAGQMDRVAELDGEIWVM
                     GYGRGRDEVDPDVWDLVVEMDLAILPNEDRRDELLIPLDPPRAERWDEITAPTLVMVG
                     EHDLPDCRQSAQLLAEALSDRDPIVVADAAHLPSLEQPESFNRYL"
     gene            1024..1635
                     /gene="rplJ"
                     /locus_tag="VFU96_09895"
     CDS             1024..1635
                     /gene="rplJ"
                     /locus_tag="VFU96_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEU5113621.1"
                     /translation="MPRPEKVQAVADIKESLEGSEAVFLTEYRGLSVKAVQELRASLR
                     ASGAEYKVVKMTLARLAADDAGISGLDEHLLGPTALAFAKSDPVATAKALKDFSKSHE
                     VFVLKAGVLSGSILGPEEVSRLAEIEPREVLLAKIAGAAKAPLVQAAGLFASFNRNAA
                     SMFQQLLEKKQSGEIEPAGGESNATLPADEPVDTETAASAEEE"
     gene            1639..2040
                     /gene="rplL"
                     /locus_tag="VFU96_09900"
     CDS             1639..2040
                     /gene="rplL"
                     /locus_tag="VFU96_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020424281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEU5113622.1"
                     /translation="MAAKTKLSTDELIATFEGMTVLELKEFLDAFEEKFEVTAAAPVA
                     VAAAPAAGGDGAAAAAVEEKDEFDVVLTGAGAQKIQVIKEVRGLTSLGLKEAKDLVDG
                     APANVLEAVDKETADKAKEVLEAAGASVELK"
     gene            complement(2119..>2350)
                     /locus_tag="VFU96_09905"
     CDS             complement(2119..>2350)
                     /locus_tag="VFU96_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113623.1"
                     /translation="DRDGKPIDPASVGLPPGIPAQVPHILTFERASRGGTDFTVTEDG
                     YTTPVVVELSRAGTDQCLDKLAALLARKVGSA"
BASE COUNT          449 a          739 c          760 g          402 t
ORIGIN      
        1 gaggtagcgg ttgaatgatt cgggctgctc caagctgggc agatgtgccg cgtcggcgac
       61 cacgatcgga tcgcgatcac tgagagcctc agccaggagc tgagccgatt gacggcagtc
      121 ggggagatcg tgctcgccaa ccatgaccaa ggtcggcgca gtgatctcgt cccaccgttc
      181 ggcccgggga gggtcgagag ggatcagcaa ttcgtcacgc cgatcctcgt tggggagaat
      241 cgccagatcc atctcgacga cgaggtccca tacgtcaggg tcgacctcgt cccgaccccg
      301 cccgtatccc atcacccaaa tctccccgtc caactcggcc acgcgatcca tctggcccgc
      361 ctcgtacgct ttcaacacct cgagccactg tgggggctgg tagtcatcga catcgaaacc
      421 gggtgcgccg gctccgatca ccacgagccc ggagacccgg tcgggtgagg cgagggcgac
      481 gtcgaatgtc gtcccccctc ccatcgagca gccgaccacc accgcctcgt cgatgtcgaa
      541 gtggtccatc accgcgagaa catcctcgtg gttggagaat cgagcatcgg tgagctcggt
      601 cgagccgtga ccgcggaggt cgagactcac aaagcgaaag ccgggtggga tttcggagag
      661 ctggtggcgc cacatccggc gatcggcgat tccggcatgg atgaagatga ccgcgctgcc
      721 ctccccgcca ctctcgtatg cgatgagagc gtctccggat ggggcatagc cagcagagga
      781 ctgcgacatc cagccaccct acccaggagc aggatcccag ccgggggagg taaacctccg
      841 gtgttgcccg aagttgccca gcgtccaccg gccggtacaa ttcatcttgc aactccaata
      901 cctcggcccc agaccgtcgg tgccttctgg ctcaatggga ctctcccgcc aacgcaggtc
      961 gctcctcgcg agccccttgt catgatgccg aggggttttg ttgtctaccg accggaggag
     1021 cgaatgccaa gaccagaaaa ggtccaggct gttgccgaca tcaaagagag tctcgaggga
     1081 tccgaagcgg tcttcctgac cgagtatcgc ggcttgtccg tgaaggcggt ccaggagctg
     1141 cgggcgagtc tgcgtgccag tggtgccgag tacaaggtcg tgaagatgac cctggcccga
     1201 ctcgccgccg acgacgccgg gatctcaggt ctcgacgagc atctgctcgg tccgaccgcg
     1261 cttgcgttcg ccaagagcga cccagtggcg acggcgaagg ccctgaaaga cttctccaag
     1321 tcgcacgagg tcttcgtctt gaaggctggt gtgctctcgg ggagcatcct gggcccggag
     1381 gaagtctccc ggctggccga gatagagccc cgtgaggtgc tgctggcgaa aatcgcaggc
     1441 gccgccaagg ctccgctcgt gcaagcggcc ggattgtttg cgtccttcaa tcgcaacgcc
     1501 gcatcgatgt tccaacagct tctggaaaag aaacagtcgg gcgagatcga gccggcggga
     1561 ggcgaatcga atgccaccct gcctgctgat gagcccgttg ataccgaaac ggccgcatcg
     1621 gccgaggagg agtgagaaat ggccgccaag accaagctca gcaccgatga gctgatcgca
     1681 acgttcgagg gcatgaccgt cctcgagttg aaggagttcc tcgatgcttt cgaggagaaa
     1741 ttcgaagtga ccgccgccgc tccggtcgcc gtagccgctg ctcccgccgc cggtggcgat
     1801 ggtgccgctg ccgcagccgt agaggagaag gacgagttcg acgtcgtcct caccggggcc
     1861 ggggctcaga agatccaggt catcaaagag gtccgtgggc tgaccagcct gggcctcaag
     1921 gaggccaagg atctcgtcga tggcgccccg gccaatgtgc tggaggctgt cgacaaggag
     1981 actgccgaca aggccaagga ggtcctcgag gccgctggtg ccagcgtcga gctcaagtaa
     2041 cgatctccac gttcaccaga agccccccga gcttgccggg gggctttctg tttcttcgaa
     2101 ggtgcttcat gggctgactc aggccgagcc caccttgcgg gcgagaaggg ccgccagctt
     2161 gtcgagacac tggtccgtgc ccgcgcgtga cagctcgacc acaaccggtg tggtgtagcc
     2221 gtcctcggtc accgtgaaat cggtcccacc gcgggatgca cgttcaaagg tcaggatgtg
     2281 aggcacctgc gcggggattc ccggtggcag gccgaccgat gcgggatcga tcggttttcc
     2341 gtcccggtcg
//
LOCUS       DASTXO010000470         3590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_765226, whole genome shotgun sequence.
ACCESSION   DASTXO010000470 DASTXO010000000
VERSION     DASTXO010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765226"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VFU96_09910"
     CDS             <1..366
                     /locus_tag="VFU96_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113624.1"
                     /translation="DGSLTELVHTEQRAVDRRFGFYMELDDASDGGTLIVPRGSFVNP
                     ELAEGFAGLEVLEAEYDPRAGADSLPETPERWLVETDSGDLVFSIVPGSDDVWWLAER
                     PNGEVVVVPNSVVPAPVSP"
     gene            363..1805
                     /locus_tag="VFU96_09915"
     CDS             363..1805
                     /locus_tag="VFU96_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113625.1"
                     /translation="MINEIAVAAALTISGSLLLTAVRRDVTAAARCWLSVPIGAAAYL
                     LVALVSLVFFDTLDPVITLIVVLGLGLVVVAVSLSKGGLGRDDLLWMMIGLGTAIFTA
                     LAARQWHLTRLTPDSLRYLLASNDLVLTGGLREMNATDLVTRQIGLPALQSLSDVVGR
                     GYLASIGPLFGVFGIGLFTWLALDVTRGGQSRRNRLILVGAAILFLASSNRLVYDSFY
                     INTHIQMASYLLIAIAGIWLAVSRGLSRWAIPSGLALAVTLVFRPEAPLVVAIVLVTV
                     AASRADMSIRLATTLPTLAVGTVWYGLGLWRYAAGGDEISPTAPVFGSLLAIVVAVIL
                     CLAGGADRARSWARWLDVAGVAGLALLLAFYVATDPAVFGMSIEATARNLVRDGFWLL
                     TWVAALALLMVAMIVQAIPDARLWTVPIIGFALLYWALPYVREGAWRVGAGDSGNRIL
                     AHYLAVTVIFLVLAAVHNRGSASMERVESV"
     gene            1819..2766
                     /locus_tag="VFU96_09920"
     CDS             1819..2766
                     /locus_tag="VFU96_09920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013154879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5113626.1"
                     /translation="MDQPSVDVIVPAKDAGQTIRPALEAVNQQTYRGVGSVVVAASDL
                     GTAAVARECGAVVVNNPTGRTPIGLNLALAQTTAEVIARVDAHAVIPPGYLARAVQVL
                     VDTGADTVGGMQVPLGSTASEKAIAAAMSSRLGAGDARYRIGGKAGPADTVYLGVFLR
                     STLERLGGYDEHFDRNQDYELNQRIRDSGGIVWFDPGLEVSYRPRGSLIALARQYFDY
                     GRWKREFARRHPGSLRLRQLAPPMLVTGLFVAIVAGFWWPWAWLAVAAYVVLLALFGL
                     TRLPSIGPGALLIPLALATMHSSWGLGFLVGRTRRDTSP"
     gene            complement(2681..>3590)
                     /locus_tag="VFU96_09925"
     CDS             complement(2681..>3590)
                     /locus_tag="VFU96_09925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5113627.1"
                     /translation="RRHWDILVLHDPETIPLGVLARLLKRRPVVFDVHEDIPATALTR
                     PWVPDLVRRPLSVLSRWVLRLGERFLIITLAEAGYSHLFTEEHAVFPNYPDTSDYPDP
                     EPESRREVVHVGDVTVVRGLDTAIEACALAGMPLRLIGPVSTDLRTELSNLTRAVGGE
                     VLFEGPLPNPEAMRIAVGAAVAIVPWKDLPNYRDSVPTKLYEYLSLGVPVVASDLPGI
                     RQAGVGDLAIILVEPGNPAALAAGIIEAVDGDMDMRAKASVDVAMVRDRYRWPDHEVT
                     DLYLALSGRRGSRDPNSNASLPGPEV"
BASE COUNT          562 a         1180 c         1162 g          686 t
ORIGIN      
        1 gacggttcat tgaccgagtt ggtccacacc gagcagcggg ccgtggatcg tcggtttggg
       61 ttctacatgg agctcgacga tgccagcgac ggagggacct tgatcgtccc gagggggtcg
      121 ttcgtcaacc ccgagctagc tgaaggcttt gccggcttgg aggtgctcga ggccgaatac
      181 gacccgagag ccggagcgga cagtctcccg gagacgcctg agcgctggct ggtcgaaacc
      241 gactcgggtg atctcgtgtt ctcgatcgtc ccgggcagcg acgacgtgtg gtggctcgcc
      301 gagcgaccga acggggaggt cgtggtcgtc cccaactctg tcgtgcccgc cccggtgtcg
      361 ccgtgatcaa cgagatagct gttgcagcgg ccctgaccat ttccggcagc ctcctcctca
      421 ccgcggtgcg aagagacgtg accgcggccg cccgatgctg gctatcggtg ccgatcgggg
      481 cggccgccta cttgctcgta gccctggtct ctctcgtgtt cttcgacacc ctcgacccag
      541 tcatcactct cattgtcgtc ctgggactcg ggttggtggt ggttgcggta tccctatcca
      601 aggggggcct cggccgagat gacttgctgt ggatgatgat cggcctcggg accgccattt
      661 tcaccgcact cgccgcgagg caatggcacc tcacccggct gacgccagac tcgctgcggt
      721 acctgctggc atcgaatgat ctcgtactca ccggggggct gcgggagatg aacgcaaccg
      781 atctggtgac ccgtcagatc ggtctcccgg cgctgcaaag cctgtccgac gtcgtcggtc
      841 gcggctacct agcctcaatc ggcccgctgt tcggggtgtt cgggatcggg ctgttcacct
      901 ggcttgccct ggacgtaacg cggggagggc agagccgccg gaaccgcctg atcctggttg
      961 gagcggcgat cctgttcctt gcttcgtcca accgcctcgt ttacgactcg ttctacatca
     1021 acacccacat ccagatggct tcttacctgc tcatcgcaat agccggtatc tggctcgctg
     1081 tctcccgtgg gctatcacga tgggccatcc cttccggtct cgccctggct gtgacactcg
     1141 tgttccgtcc cgaggctccg ctcgtcgttg ccatcgtgct ggtgaccgtt gcggccagcc
     1201 gggcggacat gtcgatcagg cttgccacga ccctcccgac tttggcggtg ggcaccgtgt
     1261 ggtatgggct cgggttgtgg cgctatgccg caggcggcga cgagatctcg ccgaccgcac
     1321 cggtattcgg gagtctcctg gccatcgtgg tggctgtcat tctctgtctc gcaggcggcg
     1381 ccgaccgcgc ccgatcgtgg gcccgctggc tggacgtggc aggagtcgcc ggcctcgccc
     1441 tactgctcgc cttctatgtg gccactgatc cggcggtgtt cggtatgtcc atcgaggcga
     1501 ccgctcgcaa cctggtcaga gacggcttct ggttgctgac ttgggtagcg gccctcgcgc
     1561 tcctgatggt ggcgatgatc gttcaagcca tccccgacgc gaggctgtgg accgtcccga
     1621 tcatcgggtt cgcgctgctc tattgggcgc tcccctatgt gcgagagggg gcttggcgag
     1681 tgggagctgg tgattccggc aaccggatct tggcccacta cttggcggtc acggtgatct
     1741 tcctcgtcct ggccgcagtg cacaacaggg gcagtgcctc catggagcgg gtggaaagcg
     1801 tgtgagaatc cgggcgtgat ggatcagccc tcggtcgacg tgatagtccc ggccaaggac
     1861 gccggccaaa ccatccggcc cgcactcgaa gcggtcaacc agcagaccta tcggggcgtc
     1921 ggatcggtgg tggtggccgc ctcggatctc ggaaccgccg ccgtcgctcg agaatgtggg
     1981 gccgtcgtgg tgaacaaccc gaccggtcgc accccgatcg gcctcaacct ggccctggcc
     2041 cagaccactg ccgaagtgat cgcccgcgtg gatgcccatg ccgtcatccc gcccggctat
     2101 ctggcccgag ccgtgcaagt cctagtcgac acgggcgccg acactgtcgg aggcatgcag
     2161 gtcccgctgg gatccacggc ctcggagaag gcaatagcgg cggcaatgtc gtcccggctc
     2221 ggcgccggcg acgcccggta tcgcatcggc ggcaaggcag gcccggccga caccgtctat
     2281 ctaggcgtgt tcctcagatc gaccttggag cgactcggtg gctacgacga gcacttcgac
     2341 cgcaaccagg actacgagct gaatcagaga atcagagact cggggggcat tgtctggttc
     2401 gatcccgggc tcgaggtgtc ctatcgaccc cggggatcgc tcatagccct ggcccgtcag
     2461 tacttcgact atgggcgatg gaagcgggag ttcgcccgtc gtcatcccgg ctcgctgcga
     2521 ctccggcagc tggcgccgcc gatgctcgtc acgggtctct tcgtggccat cgttgccggc
     2581 ttctggtggc cgtgggcatg gctcgcggtc gcggcctatg tcgtccttct cgccctgttc
     2641 gggctcaccc gactcccaag catcggacca ggcgccctgc tcatacctct ggccctggca
     2701 acgatgcatt cgagttgggg tctcggcttc ctcgtcggcc ggacaaggcg agatacaagt
     2761 ccgtgacctc gtggtcgggc cagcggtacc ggtcccgcac catcgcgacg tcgacacttg
     2821 ccttcgcccg catatccata tccccatcga cagcctcgat gatcccggct gcgagggcgg
     2881 ctggatttcc cggttccacc aggatgatgg cgaggtcgcc gactcccgcc tggcgaatcc
     2941 cgggaaggtc ggaggcgaca accgggaccc cgagtgagag gtactcgtac agcttggttg
     3001 gtaccgaatc gcggtagttc ggcaaatcct tccacggcac gatcgccact gcagctccca
     3061 cggcgatcct catcgcctca gggttgggca gagggccctc gaaaaggact tcaccaccga
     3121 ccgcccgggt cagattcgac agctctgtcc tcagatcggt actcaccggt ccgatcagac
     3181 gcaatggcat tccggccaag gcgcacgcct cgatcgcggt gtccagaccg cggacaaccg
     3241 tcacatctcc gacatggacc acttcccgcc gagactcggg ctccgggtcc ggatagtcag
     3301 aggtgtccgg gtagttgggg aacacagcat gttcctcggt gaacagatgg gagtacccgg
     3361 cctcggccag ggtgatgatc aagaatctct cccccagccg gagcacccac ctgctgagca
     3421 cggagagagg tcgccgcacg agatcaggca cccagggacg ggtgagagcg gtggccggga
     3481 tgtcctcatg tacgtcgaac accaccgggc gccgcttgag cagccgcgcc agcaccccga
     3541 gcgggatggt ctccggatcg tggagaacga gtatgtccca gtggcgccgc
//
LOCUS       DASTXO010000471         4963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1133876, whole genome shotgun sequence.
ACCESSION   DASTXO010000471 DASTXO010000000
VERSION     DASTXO010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133876"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="VFU96_09930"
     CDS             complement(<1..860)
                     /locus_tag="VFU96_09930"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEU5113628.1"
                     /translation="MNWTALLWLVLLLIANGFFVAAEFAYITARRNVLEQVPRRSAQI
                     AVGLNKNLTLSLAAAQLGITMASLVLGAVAEPAIASIFELTLGFLPLSENVIHGISLG
                     IALLIVVFLHMVIGEMAPKNITISAPERTAVLLAFPFRGFILVFRPLIAFLNASANGV
                     LRLFGVRPTDALEVGHSAEDLAVIIGTGRREGVIEDFAHRLLTGAIIFGDLDASEVMV
                     PRPDIATAPLETPVFGIQELMRVTGHSRIPIHEGDLDNVVGLIHVKDLMTDEIDRDLP
                     LSPNRLRKPLV"
     gene            complement(857..2152)
                     /locus_tag="VFU96_09935"
     CDS             complement(857..2152)
                     /locus_tag="VFU96_09935"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEU5113629.1"
                     /translation="MGAVVLLILLTGFFVAAEFALVAVDRSNVERRAQEGDRSARRIV
                     SSLRNLSFELSGAQLGITVTSLILGAIAEPTVAQLIRPVVSSIPFISEPSVPAVSLVI
                     AFILATGAAMVFGELVPKNLAIARPYFSGVWFAIPLQMVNRLLRPLIVFLNESANWTV
                     RKLGIEPREELAGVRSMEELELVIRSSGEEGRLDDDEMELLTRAITFTEKVAADAMVP
                     RVTVVGLSRHDSVAELRRASASTGHSRFPVFDEDLDDIVGVVHVKDSFNVPESRIPLT
                     PVGELSTPALQVPETSTLEYLLTQLQTAGRGMAVVVDEYGGTAGIVTIEDLLEEIFGE
                     IEDEYDTESHPESGTEEVEVLSGLLHRHEVEELIGFEWPEGRYETLGGFLVAELGRFP
                     HVGEMIRVANTAFEVLSMDGHRVDQVRVIRYGDRDGVES"
     gene            2269..2625
                     /locus_tag="VFU96_09940"
     CDS             2269..2625
                     /locus_tag="VFU96_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113630.1"
                     /translation="MPKYLVNEAAVARAEKLIEARQYVVRSEWSEVQPTAADENEFLE
                     RNDWEAYSLWHLGLTDGAHEDTKARYAFVFGDLRRLHRSGLMACRFRATQWDHKEIEL
                     ASHDLIQKLDARRGKS"
     gene            complement(2675..4084)
                     /gene="sufB"
                     /locus_tag="VFU96_09945"
     CDS             complement(2675..4084)
                     /gene="sufB"
                     /locus_tag="VFU96_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEU5113631.1"
                     /translation="MATVDLDLGAYQLGWRDDIEYAFTPRKGLDEDVIREMSAMKKEP
                     EWMLDFRLKAYQRFLRKPIPQWGGGGALNTIDFDDIYYYVKPAGEQQKDWDMVPESIK
                     ATYEKLGIPAAERKYLAGVTAQYESEVVYHRNREDLEKLGVLFTDMDTALREYPDIVK
                     EYFGTIIPPNDNKFAALNSAVWSGGSFIYVPPGVHVDQPLQAYFRINAENMGQFERTL
                     IIVDEDAYVHYVEGCSAPVYTTDSLHAAVVEIVVKEGGRCRYTTIQNWSNNVFNLVTK
                     RAAAYRNATMEWVDGNLGSRLTMKYPAVWLMEPGAHGEVLSIAFAGEGQHQDAGAKII
                     HVAPDTTSTIVSKSISKSGGRAGYRGLVRVEPGAENAKSFVRCDALILDEESRSDTYP
                     YMEIEENDAEIGHEATVSKVGEEQLFYLMSRGLTEDQATSMIVAGFIEPIVKELPMEY
                     AVEMNRLIELNMAEAGAIG"
     gene            4289..4732
                     /locus_tag="VFU96_09950"
     CDS             4289..4732
                     /locus_tag="VFU96_09950"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEU5113632.1"
                     /translation="MPVIVVGADTLQGRAVVEGLVEPGREVRAFVSDPAAGAELRALG
                     VKVALGDVSDDSHVQGSCINCFTAVLVTEAARDGRERSFAANEEQVLQGWAAAAAASG
                     VTRVIWVHDGDPPPARVKEVRTVSPGHPDLAAEVAALDDARVLPR"
     gene            complement(4756..>4963)
                     /locus_tag="VFU96_09955"
     CDS             complement(4756..>4963)
                     /locus_tag="VFU96_09955"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU5113633.1"
                     /translation="HVAAGQHIAYVGDSGNAEWTGSHTHFELAHDGGEVDPYPVLKDA
                     YARAIALRSAATRGSMRYGDAKLA"
BASE COUNT          929 a         1561 c         1562 g          911 t
ORIGIN      
        1 accagcggct tgcgcagacg attcggcgat agtggcaaat cccgatcgat ctcatctgtc
       61 atcaggtcct tgacgtgaat caagccgacg acgttgtcga gatcgccctc atggatcggg
      121 atccgggaat ggccggtgac cctcatcaac tcctggatcc cgaaaacggg ggtctccagg
      181 ggagcagttg cgatgtcggg cctggggacc atgacctcgg atgcgtcgag gtccccgaaa
      241 atgatcgctc cggtgagcaa ccggtgggcg aagtcctcga tgaccccctc cctcctgccc
      301 gtgccgatta tcactgcgag gtcctctgcc gagtgaccga cctccagggc atcggtcggc
      361 ctgaccccga agagacggag cactccattg gcgctggcat tgagaaacgc gatgaggggt
      421 cggaagacga ggatgaaacc gcggaacggg aaggcgagga ggaccgccgt tcgctcgggc
      481 gcggaaatcg tgatgttctt cggcgccatc tcgccaatca ccatgtggag gaacacgacg
      541 atgagaaggg cgatgcccag ggatatgccg tgaatcacgt tctctgatag cggtaggaac
      601 cccaatgtca gctcgaagat cgaggcaatg gccggctccg ccaccgcgcc caacaccaat
      661 gaggccatgg tgatgccgag ttgggcagca gcgaggctca gagtcaggtt cttgttgaga
      721 ccaacggcga tttgcgcgga tctccttggt acctgctcca ggacgttgcg ccgtgcggtg
      781 atgtaggcga actcggcggc gacgaagaac ccgttggcga tgaggagcag gaccagccag
      841 agcagggcag tccagttcac gattcgactc cgtcccgatc gccataccgg atcacccgga
      901 cctgatcgac gcggtgtccg tccatcgaca gcacttcgaa tgccgtattc gcgacccgta
      961 tcatctcacc gacgtggggg aaacggccca gctcggcaac caggaagccc ccgagggtct
     1021 catagcgtcc ctctggccac tcgaagccga tcagctcttc gacctcatgg cggtgcagga
     1081 gcccggacag aacctcgacc tcttccgtcc ctgactccgg gtgggactcg gtgtcgtact
     1141 cgtcttcgat ctctccgaag atctcctcta ggaggtcctc gattgtcacg atcccggccg
     1201 tcccgccgta ttcgtccacg accaccgcca tcccccgtcc tgccgtctgc agttgggtga
     1261 ggaggtattc gagggtgctc gtctctggga cctggagggc gggcgtcgac agctccccca
     1321 ccggtgtgag cgggatccgt gactcgggca cgttgaagct gtccttgacg tggaccacgc
     1381 cgacgatgtc gtcgagatcc tcgtcgaaga ccgggaatcg agagtggcca gtcgaagcag
     1441 acgccctgcg caactcggca accgaatcgt gtcggctgag cccgaccacc gtgacccttg
     1501 gcaccatcgc atccgccgcg accttctcgg tgaaggtgat ggcccgggtc aacagctcca
     1561 tctcatcgtc gtcgagacga ccctcctcac ccgaggatcg gatcaccagc tccaactcct
     1621 ccatggaccg aaccccggcg agctcttctc ttggctcgat ccccagcttt cgcactgtcc
     1681 agttggccga ctcgttgagg aagacgatca gcggccggag cagtctgttc accatctgca
     1741 gagggatcgc gaaccagaca cccgagaaat agggcctggc gatggccagg ttcttgggga
     1801 ccaattcacc gaacaccatg gcggctcccg tggcgaggat gaacgcgatc accaacgaca
     1861 cggccgggac ggatggctcg gagatgaacg gaatcgagga gaccaccggc cggatcagct
     1921 gtgcgactgt cggctcggcg atggcgccca ggatcagaga cgtcaccgtt atccctagct
     1981 gcgccccgga gagctcgaag gagaggttgc gcaacgagga gacgatgcgc cgcgccgacc
     2041 gatccccttc ctgcgccctt cgctccacgt tgctgcggtc caccgccacg agagcgaact
     2101 cggcagccac gaagaacccg gtgagcagga tgagcaagac gaccgcgccc aacccgaggt
     2161 aggtgctaat tgatcggctc cgggctcacg gttcgaacaa ggataagtct gcccggagag
     2221 cgtcgtggtt tgtcagccgg tgcggggtgg taggtgccga ccggatccat gcccaaatat
     2281 ctcgtcaacg aggccgccgt ggccagagcc gagaagctga tcgaggcacg tcaatacgtc
     2341 gtgcgcagtg agtggtccga ggtacagccg accgccgccg acgagaacga gttcctggag
     2401 cgaaacgact gggaggcgta ttccctctgg catctgggtc tcaccgatgg cgcccacgag
     2461 gacacgaaag cccggtacgc cttcgtgttc ggcgacctgc gccgtctcca tcggagcggc
     2521 ctcatggcgt gccggtttcg ggcgacacag tgggaccaca aggagatcga gctggcgtcc
     2581 cacgacttga tccagaaact ggacgcccga cgcggcaagt catagaacgc agggtcgagg
     2641 tgttgtaacg actgcctcgt tcccccgacg gcgcctatcc gatggcgccc gcttccgcca
     2701 tgttgagctc gatgagccgg ttcatctcga ccgcgtactc catgggtagc tccttcacga
     2761 tcggctcgat gaacccggcg acgatcatcg aagtcgcctg atcctcggtg agaccgcggg
     2821 acatcaggta gaacaattgc tcctcgccca ccttggacac ggtggcctcg tggccgatct
     2881 cggcgtcgtt ctcctcgatc tccatgtagg ggtatgtgtc ggaccgactc tcctcatcga
     2941 ggatcagggc gtcgcagcgc acgaacgatt tggcgttctc ggcgccgggc tcgacgcgga
     3001 ccaggccgcg ataaccggcc cgtcccccgc tcttcgagat cgacttggag acgatggtcg
     3061 acgtggtgtc aggggcgaca tggatgatct tggccccggc atcctggtgc tggccttcgc
     3121 cggcgaaggc gatggagagc acctcgccat gggcgcccgg ctccatcagc cacaccgccg
     3181 ggtacttcat ggtgagacgc gatcccaggt tcccgtcgac ccattccatc gtggcattcc
     3241 gataggcggc ggcccgcttg gtgaccaggt tgaacacgtt gttcgaccag ttctggatcg
     3301 tcgtgtagcg acagcgcccg ccctccttga ccacgatctc gaccacggcg gcgtgcagcg
     3361 agtcggtggt gtagaccggc gccgagcaac cctcgacata gtgaacgtag gcgtcttcgt
     3421 cgacgatgat cagcgtcctc tcgaactgac ccatgttttc ggcgttgatc cggaaatagg
     3481 cttgcagggg ctggtcgaca tgcacacccg gcgggacgta gatgaacgac ccacccgacc
     3541 acaccgccga gttgagcgcg gcgaacttgt tgtcgttggg cgggatgatg gtgccgaagt
     3601 attccttgac gatgtcgggg tactcacgaa gcgccgtgtc catgtcggtg aagaggacac
     3661 ccagcttctc caggtcctct cgattgcggt ggtagacgac ctccgactcg tactgcgcgg
     3721 tcacaccagc caggtacttc cgctcggcgg cgggaatccc gagcttctcg taggtggctt
     3781 tgatcgactc gggaaccatg tcccaatcct tctgctgctc gccggccggt ttgacgtagt
     3841 agtagatatc gtcgaaatcg atcgtgttca atgcgccacc gccaccccac tgcgggatcg
     3901 gcttgcgcag gaaccgctga taggccttga gccggaaatc gagcatccac tcgggttcct
     3961 tcttcatggc cgacatctct cggatcacgt cctcatcgag acccttccgc ggcgtgaagg
     4021 catactcgat gtcgtcccgc cagccgagct ggtaggcgcc gagatccagg tcaaccgtgg
     4081 ccacaggcaa tcacctcgat agttcgcttc tcacaggcct cttatatgaa cgggagtggg
     4141 acgtctggaa tttccactat cccgatagtg cgattgtacc ggaagggtgt tcctggcgaa
     4201 accggtcggc tgcggggccg gatcggagct cgctccatta tcgacgcgtc ggggcctgat
     4261 acccgacgtc gaacattagg ttccgggtat gccggtgata gtggtcgggg ccgacacgtt
     4321 gcagggtcgg gcggtcgtcg aagggctagt cgaaccgggc cgcgaggtgc gcgccttcgt
     4381 ctccgatcca gctgcaggcg ccgagctcag ggcattgggg gtcaaggtgg cgttgggtga
     4441 tgtctctgac gacagccatg tccagggctc ctgcatcaac tgcttcacgg ccgtattggt
     4501 cacagaagcg gcgcgagatg ggagagagcg ctcctttgcc gccaatgagg aacaggtgct
     4561 gcagggctgg gctgcggctg ccgccgcttc aggagtcacc cgggtgatct gggtccacga
     4621 tggtgatccc ccgccggccc gggtcaaaga ggtgcgaacg gtgtctccag gtcaccccga
     4681 cctcgctgcc gaagtggcgg ccctcgacga cgcccgcgtc ctcccccgct gagaagggaa
     4741 ccgccgatgg gatcgtcagg ccagcttggc gtctccgtac ctcatcgagc cacgtgtcgc
     4801 cgcagagcgc aacgcgatcg cacgcgcata cgcatctttt aggacaggat agggatcgac
     4861 ctcccctccg tcatgggcga gctcgaaatg ggtgtgagac ccggtccact cggcgttccc
     4921 cgagtcaccc acataggcga tgtgttggcc ggccgcgaca tga
//
LOCUS       DASTXO010000472         3343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_64989, whole genome shotgun sequence.
ACCESSION   DASTXO010000472 DASTXO010000000
VERSION     DASTXO010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64989"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VFU96_09960"
     CDS             <1..381
                     /locus_tag="VFU96_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113634.1"
                     /translation="WVPMLIFLSDFHVTWGYIAAGISGIVGLWGVLIRRRDPPPRMFY
                     YGVGLAISALLLQIVVGVIVANGGIDPGNQHVFYGVVIAVTFTFAYIYRAQFRKRPSL
                     YYGLLLLFTMGLAIRGILTFGGNF"
     gene            392..1174
                     /gene="fabI"
                     /locus_tag="VFU96_09965"
     CDS             392..1174
                     /gene="fabI"
                     /locus_tag="VFU96_09965"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020547548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEU5113635.1"
                     /translation="MLLENKKLVITGVLTDDSIAWHVARIAQEEGAEIVATGFGRGLR
                     LTERSVQRLPSTCPVLELDINDPDQVSSLVSDLGERWGRVDGALHAIAFAPEDALGGN
                     FLNTPPESAQTAFMTSAFSYKTLAVALLPLMPGGGALVTLDFDNSQAWPAYDWMGVAK
                     SALQSVTRYLARDLGPHGVRVNAVSAGPLSTVAAKSIPGFDAFDRVWKERAPLLWDGS
                     NPDPVARMVCVLLSDWAPMTSGEVVHVDGGFHAIASGGERTD"
     gene            complement(1171..2955)
                     /locus_tag="VFU96_09970"
     CDS             complement(1171..2955)
                     /locus_tag="VFU96_09970"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5113636.1"
                     /translation="MDGMTPTTHQAMARFRASLLRRASAVAWVVLIIIAAQAVRQQVF
                     TQPGFWIPMAGLAAMLVVSTATRWDRPMATRYAPWIAVAWLTGLIVGITAFALIDPLE
                     ATATPILYGIAAVSGLLLTWWAHAFMTVLIGLAISYIAIDAGTVAIADLVAPVLTVGV
                     VSAATALVGLEFEREAVRSSSRETQIDQQRLDFERLYEVSATLARAESLAEIVPHLVG
                     TICKYLDAQVGVVLLYNAERHRLEVMSPIWVNGYPLETDPIVLDVASPAIVAQVYRSG
                     RPFFVRDIDQDTDAHVLLRELGLRQALVAPLKVENLRVGAIIVGDPYVGTFNEEQLDQ
                     LGSLAAPAGLVLSQIGRYEAQAEMTRRLEEVAQMKTDFVSTVSHELRTPLTSIIGSLD
                     TVNRPELAPTQATAQQLLSTARRQAGRLQRLIDDLLVVSRIDRGAIPVHLEPVSLKLA
                     FEEVNRVVSIEPTFTIEPPDLTVEADRDHLTRILINLVENAAKYAVGSPVELYAWERP
                     NKAVIAVVDHGPGIPDDERERVFERFTQIDQSDTRSKGGTGLGLSIVKSLAEVMFGAV
                     RVEATEGGGATFVVELPLARALQKVESG"
     gene            3015..>3343
                     /locus_tag="VFU96_09975"
     CDS             3015..>3343
                     /locus_tag="VFU96_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5113637.1"
                     /translation="MALRALSDGALFAEAYGDGPPRVLALHGWGRRGSDFAPSLADFD
                     ALSVDLPGFGATPPPETVLGAEGYARLVASVLDEFEAPPVVIGHSFGGRIAVCLAAAH
                     PDRVGSL"
BASE COUNT          553 a         1040 c         1124 g          626 t
ORIGIN      
        1 tgggtcccta tgctcatttt cttgagcgac ttccatgtca cctggggtta catcgcggcc
       61 gggatcagcg ggatagtcgg gctctggggg gtcttgatca gacgtcgcga tcccccgccc
      121 aggatgttct attacggagt cggcctcgcc atctcggctc tcctcctcca gatcgtcgtg
      181 ggagtgatcg tggccaatgg tggaatcgac ccgggtaacc agcatgtctt ctacggtgtc
      241 gtaatcgcgg tgaccttcac attcgcctac atctatcgag cccagttccg aaagcggccg
      301 tccttgtact acgggttgct tcttctgttc accatggggc tcgccatcag ggggattctc
      361 accttcggcg gcaacttcta aggagatggt gatgctgctc gagaacaaga aactggtcat
      421 aaccggggtc ctcaccgacg actcgatcgc ctggcacgtg gcgaggatcg cccaggagga
      481 gggtgctgag atcgtcgcca cgggcttcgg ccgcggcctc cggctgaccg agcgatcggt
      541 gcagcgcctg cccagtactt gcccggtgct cgagctcgac atcaacgatc ccgatcaggt
      601 ctcctcactg gtctcggatc tgggcgaacg ctggggtcgg gtggacgggg ctctccacgc
      661 cattgccttt gcccctgagg acgctctcgg gggcaacttc ctgaacacgc cgcccgagtc
      721 cgcccaaacc gcattcatga cttcggcgtt ctcatacaag acgctggctg tggctctcct
      781 accgctcatg ccgggcggcg gggcgctcgt cactctcgat ttcgacaact cgcaggcctg
      841 gccggcatac gactggatgg gggtggccaa gtcggccttg cagagcgtca cccggtacct
      901 ggcccgtgac ctggggccac atggagtcag ggtgaacgcg gtgtcggcgg gccctttgag
      961 cacggtcgcc gccaagtcga tccctggctt cgatgccttc gatcgtgtgt ggaaggagcg
     1021 cgccccgctc ttgtgggacg ggtctaaccc agacccggtg gcccggatgg tgtgtgtcct
     1081 cctcagtgac tgggcgccga tgacctcggg tgaggtcgtt cacgtcgacg gtgggtttca
     1141 cgccatcgcc tcggggggcg agagaaccga ctagccgctc tccaccttct gcaaagcgcg
     1201 tgcgaggggc agctcgacga cgaaggtggc cccgccgccc tccgtcgctt cgacacgcac
     1261 cgccccaaac atcacctcgg ccaggctctt gacgatggac aggccgagac cggttccacc
     1321 cttcgacctc gtgtcggact ggtcgatctg ggtgaatcgc tcgaagaccc gttctctctc
     1381 atcgtccggg atgccgggac catggtcgac gacggcgatc acggccttgt tgggacgctc
     1441 ccatgcatac agctccacgg ggctgccgac cgcgtatttc gccgcgttct cgaccaggtt
     1501 gatcaagatc cgggtgagat ggtcgcggtc ggcctcgacg gtgaggtcgg ggggctcgat
     1561 cgtgaaggtg ggctcgatcg agacgacccg gttcacctcc tcgaaggcaa gcttcaaaga
     1621 caccggctcg agatggaccg ggattgcgcc ccggtcgatg cgggaaacca cgagcaggtc
     1681 gtcgatgaga cgctggagcc ggcccgcctg acgccgggcc gtggagagga gctgctgggc
     1741 cgtggcctgg gtcggggcga gctcgggccg gttgacggtg tccagagaac cgatgatgga
     1801 ggtgagcggt gtgcgcaact catggctcac ggtggagacg aagtcggtct tcatctgggc
     1861 gacctcctcc agacgtctgg tcatctccgc ttgggcctcg tatcggccga tctgagaaag
     1921 gacgaggccg gccggagccg ccaatgagcc gagctggtcg agctgttctt cgttgaaggt
     1981 gccgacgtag gggtcgccga cgatgatggc gccaacccgc aggttctcca ctttcagcgg
     2041 cgccaccaag gcctggcgca gccccagttc cctgagtagg acatgggcgt cggtgtcctg
     2101 atcgatgtcc cttacgaaga aggggcgacc tgaccggtac acctgggcga cgatggcggg
     2161 agacgcgacg tcgaggacga tggggtcggt ctcgagggga tagccgttga cccagatcgg
     2221 gctcatcacc tcgagacgat gacgctcggc gttgtagagc aggaccacgc cgacctgcgc
     2281 gtcgaggtac ttgcagatcg tgccgacgag gtggggcacg atctcggcca gagactccgc
     2341 ccgtgccagc gtcgcgctca cttcgtacag acgctcgaaa tcgagccgtt gctggtcgat
     2401 ctgcgtctca cgtgacgatg atcggacggc ctcccgctcg aactccagac caacgagggc
     2461 ggtggcggcg gagaccacgc cgacggtgag gaccggggct accaggtcgg cgatcgccac
     2521 ggtgcctgcg tcaatggcga tgtaagagat cgcgagcccg atcagcacgg tcatgaacgc
     2581 gtgcgcccac caggtgagga gaaggccaga gacggcggcg atcccataga ggatgggcgt
     2641 tgccgtcgcc tcgagcggat cgatcaaggc aaaggcggtg attccgacga tgaggccggt
     2701 cagccaggcg acggcgatcc acggggcgta acgggtagcc atcggccgat cccatcgcgt
     2761 ggcggtggag acgaccagca tcgcggcgag ccctgccatt gggatccaga atccgggctg
     2821 ggtaaacacc tgctgccgaa ccgcctgggc ggcgatgatg atcaggacca cccaggccac
     2881 cgccgaggcg cgtcgcagta gcgaggcccg gaaacgggcc atcgcctgat gcgtcgttgg
     2941 tgtcatcccg tccatgtcac tgaatggaag ccttccgact gtattctcgg catttccgac
     3001 cgaggactag atacgtggct ttgcgcgccc tttccgacgg cgcattgttc gcagaggcat
     3061 acggtgacgg gcccccgcgg gtgctggcgc tccacggttg gggtcgccgt ggcagcgatt
     3121 tcgccccatc attggccgat ttcgatgccc tgtcggtcga tttgcccggg tttggggcca
     3181 caccgccacc cgagacggtc ctcggtgccg agggctatgc acgcctggtt gcgtcggttc
     3241 tggacgaatt cgaggcgcct ccggtcgtga tcggccattc cttcgggggc cgaatcgccg
     3301 tttgtctggc cgcggcccat ccggaccggg tcggttcgtt gat
//
LOCUS       DASTXO010000473         9670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1207471, whole genome shotgun sequence.
ACCESSION   DASTXO010000473 DASTXO010000000
VERSION     DASTXO010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 9670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9670
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207471"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..378)
                     /gene="ffs"
                     /locus_tag="VFU96_09980"
     ncRNA           complement(111..378)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFU96_09980"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            453..1616
                     /locus_tag="VFU96_09985"
     CDS             453..1616
                     /locus_tag="VFU96_09985"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5113638.1"
                     /translation="MSARFGPNFGKVFAAALLQEMSFFLLVHFPGYLATLGITEALIG
                     VLYAAAAGVSLAFRPALGRILDLTNRRTVLLVTGVLNALVVLALAFTSVWGPLVWGLF
                     LAQRVLQIILFTTMLTYGADSIPAEHRTQGLALFGLSGLLPIALGGIAGDLVIDAFGY
                     PALFVLASAASLASWLLVWRLPLLPILGHRPRRGFWAAVAQRNLLPLWWVTLMFGIGM
                     ETVFTFTRTYVGDRAIGSTGLFFGVYSLSAASMRIVGGRHYDRVPQRPFVAGSLLAYG
                     LCLVLMAIAGNAVVIVLAAMAAGLAHGALFPILTSQVVSRARTAERGSAMSIFTSIFD
                     IALLVSAPAVGVVIDGFDYRTAFSAVAVMLGIGAVVYTLWDRWLVSSEATIPV"
     gene            complement(1618..2049)
                     /locus_tag="VFU96_09990"
     CDS             complement(1618..2049)
                     /locus_tag="VFU96_09990"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEU5113639.1"
                     /translation="MRQALKAGAEAAGHGDVPVGAVLLNETGAVVGVDHNRREELSDA
                     TAHAEILVISARARTIGDWRLNGHTLVVTLEPCPMCAGAAVLARLDRIVYGAADLKAG
                     AAWSLYNIPQDRRLNHRVDLIDGVLADECSALLSDFFADRR"
     gene            2138..2231
                     /locus_tag="VFU96_09995"
     tRNA            2138..2231
                     /locus_tag="VFU96_09995"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2174..2176,aa:Ser,seq:gga)
     gene            2243..2333
                     /locus_tag="VFU96_10000"
     tRNA            2243..2333
                     /locus_tag="VFU96_10000"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2279..2281,aa:Ser,seq:cga)
     gene            complement(2373..3518)
                     /locus_tag="VFU96_10005"
     CDS             complement(2373..3518)
                     /locus_tag="VFU96_10005"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEU5113640.1"
                     /translation="MAIGREQVLAFRLASHGLDRRIGGVVEGVARCGIQETPLGSAAL
                     ALHSRVENLTLGKLDRALRKDRSLVTLWSLRGAPYVVPSADIGVFTAGALPVDGKSFR
                     QMLGGWASAVDKAGLDPFLLLDKLVDAAKNVLDGTSMDVNDLRDRLYTRIRSLHKIER
                     PDSARDDMPEPLFRALGLKGTVCIVEGKGTNSVLALTDQWLKPTPGPIDADTMRAELA
                     RRFLHCYGPATAQHFAEWTQRSLADAKRALTLIEDDLLEVAVAGKKSLILAADDPLLD
                     SPPEAKGIRFLPVHDPYLQQRDRATLLPDQAARRRLWQAVRGPGALLVDGALTGTWRG
                     HVQGDRWEVEIEPFGRLPAKVREASSEEAERMAALKGCSSAAVNVSS"
     gene            3583..3670
                     /locus_tag="VFU96_10010"
     tRNA            3583..3670
                     /locus_tag="VFU96_10010"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3617..3619,aa:Ser,seq:tga)
     gene            3731..3820
                     /locus_tag="VFU96_10015"
     tRNA            3731..3820
                     /locus_tag="VFU96_10015"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3765..3767,aa:Ser,seq:gct)
     gene            3855..5033
                     /locus_tag="VFU96_10020"
     CDS             3855..5033
                     /locus_tag="VFU96_10020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU5113641.1"
                     /translation="MTATARAPRPNQWQWWRWAFLDLALAVIATMAGLALYNEVLGDR
                     EPTAFGLILVLVHGGSLAFRRLWPLSVVAVMLITAAIYTFGLNLPAYMLGPGILIALY
                     TAASALERRRSLIALVVVEVAMVILILVGPSFPDFGSLALFALLIGAAWFLGDVVRRW
                     RTAAIQHEQRAAELAAAREELAHRAVSEERLRIARELHDVVAHSMTVVALQAGSGRLA
                     GRDDPEAAQHALETIEKSSREALADMRRLVGVLREGEDSLTEPAPGLEDIERLVEEVG
                     RAGLEVKVSRAGPLERVPPGMALAAYRVAQEALTNVVRHAGVDRAELALDASDGALVL
                     VVDNGPPVRPVELIEGGGHGISGMEERASLYGGSLDAGATGDGGYRVEAQFPFEGDDE
                     "
     gene            5030..5695
                     /locus_tag="VFU96_10025"
     CDS             5030..5695
                     /locus_tag="VFU96_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017539452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5113642.1"
                     /translation="MTRVGIADDQALVRTGLETIIRHSEGLELVGEAGNGLEAVELAR
                     EHRPDVLLMDIRMPMVDGIEATNRIVADPDLTDVRVLILTTFDADEYVYAALRAGASG
                     FLLKDVPPEDLVAGIRVVAEGEALLAPSITRRLISEFAVRPAIADDRTVQGLQLLTGR
                     EREIMVEVGRGLSNAEIAEALVVTHATVKTHVSRILTKLGARDRAQLVVLAYESGLVI
                     PGR"
     gene            5780..6919
                     /locus_tag="VFU96_10030"
     CDS             5780..6919
                     /locus_tag="VFU96_10030"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEU5113643.1"
                     /translation="MSHHVTHTHPDRVRGARGSSPIVASEVVAEAAPVSRGNLSVTRI
                     LRWGLMVLSLVLLVRMGETSHSEISEAIGHLFTRSWWLVGAALLMEGVWVYSLSNVYR
                     SSIRGLGGSLGRGRALRISMGAFSLSRILPGGGAAGSIFAARAIVAQGNTVPRTVASM
                     LISWWVSMSTLALVVGVGTASGLAGDRVSSAHLVGPGLVLGVLAVAGTAAVAALRVPR
                     MRARVARGLSAVGSRLGVSAQPEDWEMQAMTPVPVRRLVSLVGWAALSWIVDAAALWV
                     MFLGFGVHLHPAVLLVGYGLANLINALPELTPGWLGVLESALAATYAALGVPVGVAVM
                     AVLSYRLVSYWLPVAVGIGPALGMLRGRTTNVEDHPAARELEVVA"
     gene            6919..7677
                     /locus_tag="VFU96_10035"
     CDS             6919..7677
                     /locus_tag="VFU96_10035"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEU5113644.1"
                     /translation="MLGPIGLLGGLEHYEPTLPIDRRMLDEVGVFAPEVVILPLASFK
                     GQAAAAGALAQAHWASLGARARVVIPGSGNDRIALEMIGEADVIVLPGGVPNRLMAAL
                     YGTPLLEAIIARWEAGAGVTGSSAGAIDLFERRLNLYPPDPFRLIPGFGLLHGFVVAP
                     HFDRLRVRRWFSPFLKRMDGLAVLGIDESTGLVGRDGFMQVLGSGNVTIATDESIDLY
                     STGDILDLDLVRADPFSEPSRSNHRPLLPAGAAS"
     gene            complement(7619..9295)
                     /locus_tag="VFU96_10040"
     CDS             complement(7619..9295)
                     /locus_tag="VFU96_10040"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEU5113645.1"
                     /translation="MNENLDVLADRFWLAYSEYLPTVATVRGEHRFDDQLPAYTEDWL
                     GRIAGEFGAIESHAQAIDPSGLDLQARITRDLLIQEAAVWANEIRDRFLVAAIDPYLG
                     PHTRLLSDTRQNTVQHAGQAEALLERYSQVGAYLASALDLIQETCTAGMTPARASLDR
                     VLGQLDGYLASTIDTDPFLQLVLPDPGAQEAWLARAGRLVKEVIKPAMEAFRDGLKAS
                     IAPQARPNERAGLVWMTNGQVIYGHMIHKYTQLDRSPEEIHEIGLHWATEILPPEWED
                     IGAKALGTSTMRELFERLQTDPVFRFTSEQEMLDHARATIGRAWAAVDDWFGARPETP
                     CQVVPVPASMAPAMPPAYYMQPPPDRSREGTYFLNTYKPEERDRFQFEATHFHEAIPG
                     HHFDRSLASELEGLPMFRRFSQVYAHTEGWGLYAERLADEIGLYSTEIDRLGMLTADA
                     WRSGRLVVDTGLHALGWSRERAIDWLLEWTPIGRLVIEQEVDRYIGMPGQALSYKMGQ
                     LEITRLRSHAESRLGARFDIKAFHDVMLVSGAMTLPLLGEVVDDWIGSAR"
     gene            complement(9329..9574)
                     /locus_tag="VFU96_10045"
     CDS             complement(9329..9574)
                     /locus_tag="VFU96_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113646.1"
                     /translation="MLPDAEARADLFPGLETELRSRPSSKVLRIEIGPGTAQIALDPQ
                     DDGRVRITIAHDRLPTPKAVEEWKGYWFDWLGAVDEA"
BASE COUNT         1494 a         3122 c         3192 g         1862 t
ORIGIN      
        1 agacgctttt ctcgggccac gccctctccg ccgagcaggt ctggcgcatg ggcccattcg
       61 gcttcgtgta cggggtgcgt ctgcgaaaag gacctctgtc ggaggtttga tcgtgccccc
      121 gccgtcgaga ccttgcggcg ccggtgattg gcgggcgtgg actcgggtca ggccgccctg
      181 cgtcacccgg agcggtccac ttagggctgc ttccttccgg atctgacccg gttcgtggtg
      241 ctccgccgcg caggacccga ccgtcaacgt cccgtgtcca ccatcatccg gcgtcacgtg
      301 gaccctcggg cggggaactc agccccgcat ggagaggatt tcgggtacag ggcaccccta
      361 gcgccccgcc tatcacgatc agtcaccagc gtaggcgagc acggattatt acggatatag
      421 ccatcgcgga cggccctaac gttccgtcgg ctgtgtccgc ccgcttcggt cccaatttcg
      481 gcaaggtctt cgccgctgcc ctgctccagg agatgtcatt ctttctcctg gtccatttcc
      541 ccggatatct ggcgacgctc gggatcacag aggcgctcat cggggtcctt tacgccgcgg
      601 ccgcgggggt atcgctcgct ttccggcctg ccctgggccg aatcctcgac ctgaccaacc
      661 gtcgcactgt gctcctcgtc accggtgtgc tcaacgcctt ggttgtgctg gccctcgcgt
      721 tcaccagtgt ttggggacca ctcgtctggg ggctgtttct ggcccaacgg gtcctccaga
      781 tcatcctctt caccaccatg ctcacctacg gagcggactc gatcccggca gagcaccgca
      841 cgcagggctt ggccctgttc ggcttgtccg gtctcctccc aatagcgctc ggtggcatcg
      901 ccggtgacct ggtcatagat gccttcggct acccggctct cttcgtcctg gcttcagctg
      961 cgagtctcgc ctcctggctc ctcgtgtggc gcttacccct tttgccgatc cttggtcacc
     1021 gtcctcgacg tggcttctgg gcggccgtcg cccaacgaaa cctcctcccc ctgtggtggg
     1081 tgacgctcat gttcggcatc ggcatggaga ccgtgttcac cttcacccgg acgtacgtgg
     1141 gggatcgggc catcggatcg accggcctct tcttcggtgt ctacagcctg tccgcggcct
     1201 cgatgcggat cgtcggcgga aggcactacg accgagtccc gcaacgcccg ttcgtcgccg
     1261 gatcgctcct tgcctacggg ctgtgtctcg tcctgatggc gatagcgggg aatgccgtgg
     1321 tcatcgtctt ggccgccatg gcggcgggtc tggctcacgg ggctttgttc ccgatcctga
     1381 ccagtcaggt ggtcagccgg gcacgaaccg ccgagcgtgg ttcggccatg tcgatcttca
     1441 cctccatatt cgacatcgcc cttctcgtct ccgcgccggc cgttggagtg gtgatcgatg
     1501 gcttcgacta ccgcacggcg ttctcagcag tcgcggtgat gctggggatc ggcgccgtgg
     1561 tctacacgct ctgggaccga tggctcgtct cttcggaagc cacgattcct gtctgagtca
     1621 acggcgatcg gcgaagaaat cgctgaggag ggccgagcac tcgtcggcga ggacgccgtc
     1681 gatcaggtcg accctgtggt tcaaccggcg gtcctggggg atgttgtaga gcgaccaggc
     1741 cgctcccgcc ttgaggtctg cagcgccata gacgatgcgg tcgagacggg ccaggactgc
     1801 ggccccggcg cacatcgggc agggctcgag cgtgaccacc agggtgtggc cattgagccg
     1861 ccaatcgccg atcgttcgtg cccgggccga aatgaccagg atctcggcat gtgcagtggc
     1921 gtcggacagc tcctcacgcc gattgtgatc gaccccgacc acggccccgg tttcgttgag
     1981 tagcaccgcg cccaccggaa catcgccatg gcccgccgct tctgctccgg ccttcagggc
     2041 ctgccgcatc gggtcgatgt agcgatcgga gggcagcggc tcagccatgg gggtcgatta
     2101 tcctctatct gcctgccggg tccggcaagg cagctccgga gggatcgcat agtccggtct
     2161 agtgcgcggc actggaaatg ccgttggggg ttcacgctcc ctcgagggtt caaatccctc
     2221 tccctccgcc aaggccgccc ctggaggggt cgcatagtcc ggtctagtgc gcggcactcg
     2281 aaatgccgta gggggctgac gcctcctcga gggttcgaat ccctccccct ccgcaacgct
     2341 gctaccgcga acggccctgg tgaactccgg ggtcagctcg acacgttgac cgcagccgat
     2401 gagcatccct tgagggcggc catcctctcc gcctcctcgg acgacgcctc tcgcaccttg
     2461 gcagggagcc ggccgaatgg ctcgatctcg acctcccagc ggtctccctg cacgtggcca
     2521 cgccaggtgc cggtgagcgc gccatcgacc aggagggcgc cagggccgcg gactgcctgc
     2581 cagagccgtc ggcgagcggc ctgatcgggc agaagcgtcg cccggtcccg ttgctggaga
     2641 taagggtcgt gaaccgggag gaaacgaatt cctttcgcct cgggcggcga gtcgagcagc
     2701 gggtcatccg cggccaggat gagactcttc ttgcccgcca ccgcgacttc gaggagatcg
     2761 tcctcgatga gcgtcagggc ccgtttcgca tcggccaggc tgcgctgagt ccactcggcg
     2821 aaatgttggg cggttgccgg cccgtaacaa tgcaggaacc ggcgggccag ctccgctcgc
     2881 atcgtgtctg cgtcgattgg ccccggcgtc ggtttcagcc actggtcggt aagggcgagc
     2941 acggagttgg tgcctttccc ctcgacgatg cagacggttc ccttcaaacc gagggctcga
     3001 aacagtggct cgggcatgtc gtcccgggca gagtccggcc gctcgatctt gtgaaggctg
     3061 cggattcggg tgtacagccg gtccctcagg tcgttgacat ccatgctcgt gccatcgagg
     3121 acgttcttcg ccgcgtcgac gagcttgtcg agcagtagga atggatcgag gccggccttg
     3181 tccaccgcgg aggcccagcc acccagcatc tgacggaacg atttaccgtc gaccggtaac
     3241 gcaccggcgg tgaagacccc gatgtcggcg gaggggacga cgtacggagc gcctcgcaga
     3301 ctccacagtg tcaccagaga ccgatccttc ctcagggccc tgtccagctt ccccagtgtg
     3361 agattctcga cccgggagtg caaggcgagc gccgcggagc caagtggtgt ctcctgaatg
     3421 ccgcaccgtg ctacgccctc gaccacacct ccgatccggc ggtcgagccc gtgactggcg
     3481 agacggaagg cgaggacctg ctcgcgccct atagccaccg aaggatgcta cgacgagggc
     3541 gagatcggcc ccccgggtac cctgatgcag cgcacgtcgc ctggaggggt gtccgagtgg
     3601 tctaaggaga cggtcttgaa aaccgttggg cccgatccgg gtctcgtggg ttcgaatccc
     3661 accccctccg cccgactcag ccatggaatc gagaacggat aacgtacaca tagcggccga
     3721 ggccgctcat ggagaggtgg ccgagtggtc gaaggcgctc gcctgctaag cgagtagggg
     3781 gtgttaagcc tcctcgaggg ttcaaatccc tccctctccg ctgatcacgc ccatcgaata
     3841 gaacggcgaa tcgaatgact gccaccgcca gagcaccgcg cccgaaccag tggcagtggt
     3901 ggcgttgggc cttcctcgat ctggcgctcg ccgtcatcgc caccatggcg gggttggccc
     3961 tgtacaacga ggtccttggc gatcgggagc caacagcttt tgggctgatc ctggtgctcg
     4021 ttcacggggg ctcgctcgcc ttcaggaggc tgtggccctt gtcggtcgtc gcggtgatgc
     4081 tgatcacagc cgcgatctac acctttggtc tcaacctgcc ggcctacatg ctggggccgg
     4141 gcattctgat cgcgctctac accgcggcct cggcgttgga acggcgtcga tcgttgatcg
     4201 ctctggtcgt cgtcgaggtg gcgatggtca tcctgattct cgttggtccg agcttccccg
     4261 acttcggctc gctggccctc ttcgcactcc tgattggggc ggcatggttc ctcggcgacg
     4321 tcgtgcgcag atggcgaacg gcggcgattc agcacgaaca gcgtgcggcc gagctggccg
     4381 cggcccggga ggaactggct caccgggcgg tgagcgaaga acggcttcgc atcgcgcgtg
     4441 agctccacga cgtggttgcg cacagcatga ctgttgtcgc cttgcaggca ggcagtggtc
     4501 gcttggcggg ccgcgatgat cccgaggcgg cccagcacgc tttggagacg atcgaaaagt
     4561 cgagccgtga ggcgctcgcc gacatgagac gtctggttgg ggtgctccgg gagggagagg
     4621 attcgctcac cgagcctgcg cccggcctcg aagacatcga gcggctggtc gaagaggtag
     4681 ggcgcgccgg gctggaagtg aaagtcagcc gtgccgggcc cctggagcgg gttcctcccg
     4741 gcatggccct ggccgcctac cgcgtcgctc aggaggcgtt gaccaatgtg gtgcgtcacg
     4801 ccggcgtgga ccgagcggag ctcgcgctcg acgcgtccga cggcgccctc gtgctcgtcg
     4861 tcgacaacgg ccctccggtc aggccggtag agctcatcga gggcggaggc catggcatct
     4921 cggggatgga ggagcgagct tctctatacg gaggatcttt ggatgccggg gcgaccggcg
     4981 acggcggata tcgggtcgag gcgcaattcc cgttcgaggg ggacgacgaa tgacgagagt
     5041 cggcatcgcc gacgatcagg cgttggtgcg taccggtctg gaaaccatca tcagacactc
     5101 ggagggcctc gagctggtcg gcgaggcggg caacgggttg gaggctgtgg agctcgcccg
     5161 agagcatcgg ccagacgtcc tcttgatgga catccggatg ccaatggtcg acgggatcga
     5221 ggcaaccaat cggatcgtgg ccgatccgga cctgaccgat gtccgtgtcc tcatcctgac
     5281 cacgttcgat gccgacgagt atgtctacgc tgccttgagg gctggcgcca gtgggttctt
     5341 gctcaaggac gtcccacccg aggatctcgt cgctggcatt cgtgtcgtcg ccgagggcga
     5401 ggcattgttg gctccttcga tcacccgtcg cctgatttcc gagttcgcag ttcgccccgc
     5461 catcgcagac gaccggaccg tccagggact gcagctcctg acggggcgtg agcgcgagat
     5521 catggtcgag gtcggtcgtg gtctgagcaa cgccgagatc gccgaggctc tcgtagtgac
     5581 tcatgccacc gtgaagacgc atgtatcccg gatcctcacc aaactcggcg cccgcgatcg
     5641 agcccagctg gtcgtgctcg cctatgagtc agggttggtg atccccgggc gctgaaggcc
     5701 gtcgtacatc tggagatgta cgcccgattg cattctcgcg ggcgacgacg cgcgaggccc
     5761 ctctccatac gttggcgaca tgtcacacca tgtcacccac acccaccccg atcgggtcag
     5821 gggagcacga gggtcgtccc cgattgtcgc ctcggaggtg gtcgccgagg ctgctccagt
     5881 gagccgtggc aacttgtcag tgacccgaat cctgcgatgg gggctgatgg tcctttcgct
     5941 cgtcctcctc gttcggatgg gcgaaaccag ccattcggag atcagcgaag ccatcggtca
     6001 cctattcacg aggagctggt ggctggtcgg ggccgcgctc ctgatggagg gcgtctgggt
     6061 ctacagcctg tccaacgtgt atcggtcatc gatacggggt ctgggcggca gcctgggccg
     6121 tggtcgggcc ctccggatct cgatgggcgc cttttccttg agtcggatcc tccccggtgg
     6181 tggagctgca gggagcatct tcgccgccag ggcgatcgtg gcccagggaa acacagtccc
     6241 gagaaccgtc gcctcgatgc tcatctcctg gtgggtctcg atgtcgactt tggctctcgt
     6301 ggtgggagtc ggcactgcct cggggctcgc cggagaccga gtcagttcgg cccacctggt
     6361 cggaccgggc ttggtcttgg gtgtcctcgc ggtggcgggg accgcggcgg tcgctgcgct
     6421 ccgcgttcca cgtatgcgtg cccgtgtagc gcgagggctg agtgcggtcg gctcccggct
     6481 cggcgtgtct gctcagcccg aggactggga gatgcaggcg atgacaccag tgcccgtgcg
     6541 ccggttggtt tcgctcgtcg gttgggcggc gctgagctgg atcgtcgatg ccgcggcgct
     6601 gtgggtgatg ttcctcggct ttggcgtcca cctgcacccg gcggtcctcc tcgtcggcta
     6661 cggcctggcc aatctgatca acgccctccc cgaactgacc cctggctggc tcggcgtcct
     6721 cgaatcggcg ctcgcagcca cctatgccgc cctaggagtg ccggtcggtg tcgcggtgat
     6781 ggcggtgctg tcctaccggc tcgtgtcgta ctggctgccg gtggcagtgg gtatcgggcc
     6841 tgccctcggc atgctgcgcg gtcggaccac gaacgtcgaa gaccatccag cagcccgcga
     6901 gctcgaggtg gtggcgtgat gctcgggcca atcgggctgt tgggcggtct ggaacattac
     6961 gagccgaccc tgcccatcga ccggcgcatg ctcgatgaag tgggtgtgtt cgcgcccgaa
     7021 gtcgtgatcc ttccactcgc ttcgttcaag ggccaggcgg ccgcagccgg tgccctggct
     7081 caggcccact gggcgagtct gggagcgagg gcacgcgtcg tgatccccgg ctccggaaac
     7141 gatcggatcg cgctggagat gattggggag gccgacgtga tcgttcttcc cggaggcgtg
     7201 cccaacaggt tgatggccgc cctctacggc acacctttgc tcgaggcgat catcgcccga
     7261 tgggaagccg gcgccggtgt gaccgggtcg tcggctggcg ccatcgacct gttcgagcgt
     7321 cgtctcaatc tctatccccc cgatccgttc cgcttgattc ccgggttcgg gctcctgcat
     7381 ggattcgtgg tcgctcctca tttcgaccga ctgcgtgtcc gacgctggtt ctccccgttc
     7441 ctgaagcgga tggatggtct cgcggtgctc gggatcgacg aatcgactgg tctcgtcggg
     7501 cgggacgggt tcatgcaggt cctcgggagc ggaaatgtga cgatcgccac cgacgagtcg
     7561 atcgacctct actcgacagg ggacatcctc gatctggacc tggtgcgggc cgaccccttc
     7621 agcgagccga gccgatccaa tcatcgacca cttctcccag caggggcagc gtcatagctc
     7681 cgctcacgag catgacgtcg tgaaaggcct tgatgtcgaa acgggcaccg agccgactct
     7741 ctgcatggga tctcagccgg gtgatctcga gctggcccat cttgtaggac agggcctggc
     7801 caggcatgcc gatgtaccgg tcgacttcct gttcgataac cagcctccca atgggcgtcc
     7861 actccagcag ccagtcgatc gctctctccc gggaccagcc gagggcatga aggccggtgt
     7921 cgacgaccaa acgtccggac ctccaagcgt cggcggtcaa catcccgagc cggtcgattt
     7981 cagtcgagta gagccctatc tcgtcggcca aacgctcggc gtacaacccc catccttcgg
     8041 tgtgggcgta gacctgggag aagcgccgga acatcggcaa gccttcgagt tcgctggcga
     8101 gtgaccggtc gaaatgatgg ccggggatcg cctcgtggaa gtgggtggcc tcgaactgga
     8161 aacggtctcg ctcctcaggc ttgtaggtgt tgaggaagta ggtcccctcc ctcgagcggt
     8221 ctggtggcgg ttgcatgtag taggcaggcg gcatggcagg cgccatcgag gcggggacag
     8281 ggaccacctg acagggtgtc tccggcctcg ctccgaacca gtcatccacc gcggcccaag
     8341 ctcgcccgat ggtggccctg gcgtgatcga gcatctcctg ctcagacgtg aatcggaaca
     8401 cagggtcggt ttgcagacgt tcgaagagct ctctcatcgt gctggttccg agagccttcg
     8461 caccaatgtc ttcccattcc ggaggcagga tctcggtggc ccagtgcagg ccgatctcat
     8521 ggatctcctc ggggctgcgg tcgagttggg tgtacttgtg gatcatatgc ccatagatga
     8581 cctgaccgtt ggtcatccag accaggcccg cccgctcatt gggccgagcc tgtggcgcga
     8641 tgctggcttt caggccatca cggaatgcct ccatggccgg cttgatcact tctttgacca
     8701 aacgccccgc cctcgccaac caagcctctt gcgctccggg atcgggaagc accagctgca
     8761 ggaaaggatc ggtgtcgata gtggacgcca gatacccgtc cagctggccg agtacccgat
     8821 cgaggctggc ccgggcaggg gtcatcccag cggtgcaggt ttcctggatc agatccaggg
     8881 cgctcgccag gtacgccccg acctgggagt atcgctcgag aagggcttcg gcctgaccgg
     8941 cgtgttggac cgtgttctgc cgggtgtcgg agagcagacg ggtgtgaggc cctaggtaag
     9001 gatcaatcgc agccaccagg aatcgatccc tgatctcgtt ggcccatacc gccgcctcct
     9061 ggatgagcag gtcgcgcgta atccgggcct gtaggtcgag tcccgacgga tcgatcgcct
     9121 gagcgtgact ttcgatagcg ccgaactcac ctgcaatccg gcccaaccag tcctcggtgt
     9181 aagcgggcag ttggtcatcg aaccgatgct ctcctcgcac ggtggcgacg gttggcaggt
     9241 actccgagta ggccagccag aaacggtcag ccaggacgtc gaggttttcg ttcacgagca
     9301 cattctgacg cggatcggac gaaccctctc aggcctcgtc gaccgctccg agccagtcga
     9361 accaatagcc cttccattcc tcgacggcct tgggcgtcgg gagccgatcg tgggcgatcg
     9421 tgatcctgac ccggccgtca tcctgaggat cgagggcgat ctgcgccgtt ccgggcccga
     9481 tctcgatgcg gagcaccttg gagcttggcc gcgagcgaag ctcggtctcc aggcccggga
     9541 acaggtcggc ccgggcttcg gcgtcgggca gcatctccct gatcagcgcg ggacttgcgt
     9601 cgcctcaccg tcatcccagg agacggtgct ccatggcgta gagggcggct ccagctcgtg
     9661 tcgaaacctc
//
LOCUS       DASTXO010000474         3400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_912611, whole genome shotgun sequence.
ACCESSION   DASTXO010000474 DASTXO010000000
VERSION     DASTXO010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912611"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VFU96_10050"
     CDS             complement(<1..762)
                     /locus_tag="VFU96_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113647.1"
                     /translation="MTITFLRVRPAGKLAEPTATSRRREGTTAVEMSSPTSAELTAAH
                     KAGILVLSESVTGAEPSAWVVRPDEALALADRGVPGWRLTVVAEGPRESILDALARSG
                     ARFARSTRSEIAEIVALSALPGVDFSVSVFVDDVSQALRAVDQGATDLLLRDWDVEGI
                     GELRTALGARRLVERTAFPPGVEIDEARALLPAESFAAWLAQVDGSGFARPRHEWAPG
                     KDLPPPVPDDRLSAEWLDRSWVDGAAEPFPGSAGEL"
     gene            complement(759..1898)
                     /gene="cofG"
                     /locus_tag="VFU96_10055"
     CDS             complement(759..1898)
                     /gene="cofG"
                     /locus_tag="VFU96_10055"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF004884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HEU5113648.1"
                     /translation="MIHPAPDPDLALRYLEGDLDDAALFAHARALRDEGRGNVVTYSR
                     KVFIPLTTLCRDRCTYCTFAKPPGAGGMYLEPDEVLGIARAGDEAGCTEALFTLGDRP
                     EERWPQAASFLADRGLSSTIDYVAEMSALVRAETSLFPHANPGLLADDDLRQLRPTNP
                     SMGMMLENISPRLMETGMPHYGTLSKDPALRVATIEAAGRVKVPFTTGILVGIGESRR
                     EIVDSLFALRDLARSTGAIQEVIIQNFRAKADTPMRHSAEPVPRWFARVVALARWIMG
                     ADVNVQVPPNLTGHFEIYLDAGINDWGGVSPLTIDWVNPEAPWPHLDELRERTEAAGF
                     ELRPRLPVYPEYIVEDWIDKRLYADVVAAADRDGLALITETSGAR"
     gene            complement(1918..2697)
                     /locus_tag="VFU96_10060"
     CDS             complement(1918..2697)
                     /locus_tag="VFU96_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003935068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5113649.1"
                     /translation="MVEVSVEGKVVLVTGASRGIGQATAAEFANSGAAGITITSRKPE
                     NIASAAAELIESGVEPERLLALPARADSEESAEEAVAATMERFGSCDILINNAGTNPS
                     AGPLMDVDLGALDKTWAVNLRAPLLWVRAAYRASMEKHGGSIVNVSSVGGLRPSPITG
                     AYNVSKAGLVHMTRQLALELAPSIRVNAVAPAVVKTRLSEMLWQDEAAAAGMHPLGRL
                     GTPEDIARAIVFLASDAADWMTGVILPVDGGMSEASSSGIA"
     gene            complement(2713..3210)
                     /locus_tag="VFU96_10065"
     CDS             complement(2713..3210)
                     /locus_tag="VFU96_10065"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEU5113650.1"
                     /translation="MDPELINKVTWKIPNALALIGSASGDEWNGMTASWVTQVSMEPV
                     LIGVGVDNKAVTHRLITEGGSFTVNLWPSDDTRVFVKFSKPATREDGTLNGRPVRPGV
                     TGAPIFEEALAWMDCEVRHAIDLGTHTFFVGEIVDAGVDDDDRQAASMSDTRMKYGGV
                     RRGGH"
BASE COUNT          581 a         1158 c         1064 g          597 t
ORIGIN      
        1 cagctcccct gcggatccag gaaacggctc cgccgcccca tctacccagg accggtccaa
       61 ccactccgcc gacaatcgat cgtccggcac cgggggcgga aggtccttcc cgggcgccca
      121 ttcatggcgg ggccgggcga aacccgagcc atccacctgg gccagccagg cggcgaacga
      181 ctcggcgggt agcagagctc tcgcctcgtc tatctcgacg ccgggaggaa acgcagtcct
      241 ctccaccaat cggcgggccc ccagagccgt ccgcagttca ccgatccctt cgacatccca
      301 gtcgcggagc agcaggtcgg ttgcgccctg gtcgaccgcc cgcaacgcct gagaaacatc
      361 gtcgacgaac accgacaccg agaagtcgac tccgggcaaa gcggacaatg cgacgatttc
      421 ggctatctcc gagcgtgtgg aacgggcgaa gcgggcccct gacctggcga gtgcgtcgag
      481 gatggactcg cggggtccct cggcgacgac tgtcagccgc cagcccggca cccccctatc
      541 ggcgagagcc aaggcctcat cgggcctgac cacccaggca gaaggctccg cgccggtgac
      601 actctccgag agcaccagga ttccggcctt gtgcgcggcg gtgagctcgg cgctcgtggg
      661 cgacgacatc tcgacggcgg tagttccctc ccgacgccgg ctcgttgccg tcggctcggc
      721 cagcttccct gccgggcgca ccctgagaaa cgtgatcgtc atcttgctcc tgatgtctcg
      781 gtgatgaggg ccaggccgtc cctgtcggcg gccgccacta catcggcgta caacctcttg
      841 tctatccaat cctcgacgat gtactccgga tagacgggca gacggggccg cagctcgaag
      901 cctgcagctt ccgtgcgctc ccgcagctcg tcgaggtggg gccatggcgc ctcgggattc
      961 acccagtcga ttgtcagcgg ggagacccct ccccagtcgt tgatcccggc atcgaggtat
     1021 atctcgaaat gcccggtgag attgggtggc acctgcacgt tgacatcggc gcccatgatc
     1081 catcgagcca gggccaccac gcgtgcgaac cagcgcggca cgggctcggc ggaatgccgc
     1141 atcggggtgt cggccttggc cctgaaattc tggatgatca cttcctggat cgccccggtc
     1201 gagcgagcca ggtcgcgcaa ggcaaagagg gagtcgacga tctctctcct cgattccccg
     1261 atcccgacca ggatcccggt ggtgaagggg accttgaccc gcccggccgc ctcgatggtc
     1321 gcaacccgga gagccggatc cttggagaga gtcccgtagt gaggcattcc tgtctccatg
     1381 agccgcggtg agatgttctc cagcatcatc cccatcgacg gattggtcgg gcgcaactgc
     1441 ctcaggtcgt cgtcggcgag gagtccggga ttggcgtgtg ggaagagaga ggtctcggcc
     1501 cggaccaatg cagacatctc ggccacgtag tcgatcgtgg aactgaggcc ccgatcagcg
     1561 aggaacgagg cggcttgcgg ccagcgctcc tcgggccggt caccgagggt gaagagcgct
     1621 tcggtgcacc cggcttcgtc ccccgccctg gcgatgccca gcacctcatc cggctcgaga
     1681 tacataccac ccgccccggg tggcttggca aacgtgcaat atgtgcaccg gtcgcggcac
     1741 agtgtggtca gagggatgaa gactttgcgg ctgtaggtca cgacgttgcc ccgaccctcg
     1801 tcacgcaggg cccgggcgtg tgcgaacagg gcggcgtcgt ccaggtctcc ttccagatag
     1861 cgaagggcga gatcggggtc aggtgccggg tggatcactg ctctgctcgt ttcttgctca
     1921 ggctatgccc gatgacgacg cttcactcat gcccccatca accgggagga ttacgcctgt
     1981 catccaatcg gccgcatcgg aggcgaggaa gacgatggca cgggcaatgt cctccggagt
     2041 ccccagacgt ccgagggggt gcattcccgc cgccgccgcc tcgtcctgcc agagcatctc
     2101 cgagagcctc gtcttgacca cggcgggggc aacggcgttg acccggatcg agggcgccaa
     2161 ctcgagggca agctgtcggg tcatgtgaac caggcccgcc ttcgacacgt tgtatgcgcc
     2221 ggttatgggg cttggcctga ggccgcccac ggaagagacg ttgacgatcg agccgccgtg
     2281 tttttccatc gatgcccggt aggccgccct gacccagagc agcggagccc tcagattgac
     2341 cgcccacgtc ttgtccagag ccccgagatc gacatccatc agtggcccgg cggatggatt
     2401 ggtgccggcg ttgttgatga ggatgtcgca cgagccgaat cgctccatcg tcgccgcgac
     2461 cgcctcctcg gcggattcct cggaatcagc ccgggccggc aacgccagca acctctcagg
     2521 ttccaccccc gattcgatca actcggctgc tgccgatgcg atgttctccg gttttcgaga
     2581 tgtgatcgtg atgccggcgg ccccactgtt ggcgaattcg gcagccgtcg cctggccgat
     2641 tccacgactg gccccggtga cgagcacgac ttttccctca accgagactt ccacgatcct
     2701 cctcgagacg gatcaatggc cgcccctcct cactccgccg tatttcatcc tcgtgtcaga
     2761 catcgacgcc gcctgccggt cgtcgtcgtc gacgccggcg tccacgatct cgccgacgaa
     2821 gaacgtgtgg gtccccaaat cgatcgcgtg tcggacctcg cagtccatcc aggccaatgc
     2881 ttcctcgaat atcggggcgc cggtcacacc cggtcgaacc ggcctcccgt tgagggtgcc
     2941 gtcctccctg gtggccggct tggagaactt gacgaagacc cgggtgtcat ctgaaggcca
     3001 caggttcacg gtgaaggacc caccctcggt gatcagccga tgagtgacgg ccttgttgtc
     3061 gacgccgacg ccgatgagga ccggctccat cgagacctgc gtgacccacg atgcagtcat
     3121 cccattccac tcgtcaccag aggcggagcc gatcaaagcc aacgcgttcg ggatcttcca
     3181 ggtgaccttg ttgatgagtt cggggtccac gtgactcgca tgggcaaaag cggttccgac
     3241 accctaatct cagtcgggtg cgcatcctga tcgccacaac cggggttctt ccacccgccc
     3301 cggtggccga cttgtgccgg cgtctgcact ccgatgaggg gagcgtcacg gtcatgaccg
     3361 tggtccgggt tcctcgctcc ttccttgagg ccctggacga
//
LOCUS       DASTXO010000475         2205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1649327, whole genome shotgun sequence.
ACCESSION   DASTXO010000475 DASTXO010000000
VERSION     DASTXO010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649327"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VFU96_10070"
     CDS             <1..378
                     /locus_tag="VFU96_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113651.1"
                     /translation="EAQIGFTYISSGRLTEARDALSRSFDVFRGMNYREGLCYALEAA
                     ASLSFTEGQTELGMIALGAAEEVRERIGLHPWPLIKWLFDALSSMADSLDDPVLKAAR
                     HNGRQLNPFDAAALVLDAAPVPV"
     gene            complement(399..1445)
                     /locus_tag="VFU96_10075"
     CDS             complement(399..1445)
                     /locus_tag="VFU96_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthetase"
                     /protein_id="HEU5113652.1"
                     /translation="MKLGLVVNDVMSEKPEYSTTRLALAAHKRGHEVWLMGVGDLAHT
                     SSGEVTARARGPLDKTYRSLKNFLEDVQGEDVDPELITVDELDILMLRNDPAEDAIER
                     PWAQASGVLFGQLVTAAGVVVVNDPVHLADAINKTYFQHFPEEIRPRTLISRTVGDIK
                     DFVDELGGRAVLKPLQGSGGQGVFMIGGKKGQNLNQIIETITRDGYVVVQEFLADADK
                     GDVRLFVMNGVPLEVDGKIAAFRRVNEADDVRSNMHVGGKAQKAKITEEMLHVVDLAR
                     PKLIEDGMFLVGLDIVGDKLMEANVFSPGGLGSASGLHEVDFAGAVVEALERKVQIRA
                     TYGRSLPNSSLATM"
     gene            complement(1442..>2205)
                     /locus_tag="VFU96_10080"
     CDS             complement(1442..>2205)
                     /locus_tag="VFU96_10080"
                     /inference="COORDINATES: protein motif:HMM:NF019626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine/phenylalanine carboxypeptidase
                     domain-containing protein"
                     /protein_id="HEU5113653.1"
                     /translation="GPAADVYVDAHELAGRANVELEHYRRRFPELSTLVHVSETVAGV
                     MVENGDLFIGVDTSIAGDRVEQLLHHEIGIHVVTYVNGSAQPIRMLSRGLAGYEETQE
                     ALGVLAEHLSGGLRPKRLRAFALRVVAAHSVSGRATFRQTYDTLIDLGSGRNRAFTTA
                     MRAHRSGGMTKDALYLGGLTRLLTYLQEGGRLDPLLVGKISLADEPLVADLLERGVLV
                     EPPLRPRFLESGEALDRLARIQDGAGILELGGVAA"
BASE COUNT          362 a          747 c          694 g          402 t
ORIGIN      
        1 gaggcgcaaa tcggcttcac ctacatctct tcgggccgtc tcaccgaggc gcgggatgcc
       61 ctcagccgct ccttcgacgt gttccgcggc atgaactatc gagaaggtct ctgttacgcc
      121 ctggaggccg cggcctcact ctcgttcacc gaggggcaga cggagctcgg catgatcgcg
      181 ctgggcgccg cggaagaggt cagggagcgg atagggctcc acccctggcc cctgatcaag
      241 tggctgttcg acgctctctc ctcgatggcc gactcgctcg atgacccggt gctcaaggcg
      301 gcccgccaca acgggcgtca gctcaacccc ttcgatgcgg ccgccctcgt attggatgcg
      361 gctccggtgc cggtctgagg aagacgatgg cttccccgct acatggtggc gagggaggaa
      421 ttgggcagtg accgcccata agtggcccgg atctggacct ttctctccag ggcctcgaca
      481 acggcgccgg cgaagtcgac ctcgtggagg ccgctggcgc tgcccagccc gcctgggctg
      541 aagacattcg cctccatcag cttgtccccg acgatgtcga gcccgaccag gaacatccca
      601 tcctcgatca gcttggggcg tgccaggtcg accacatgga gcatctcctc ggtgatcttc
      661 gccttctgcg ccttgccccc gacgtgcatg ttggaacgaa catcgtcggc ctcgttgacc
      721 cggcggaagg cggcgatctt gccgtccacc tccagcggca ctccgttcat gacgaagaga
      781 cgaacgtcgc ctttgtcggc gtccgcgaga aactcctgga ccaccacgta gccatcccgg
      841 gtgatggtct cgatgatctg attcaggttc tgacccttct tcccgccgat catgaagacg
      901 ccctgtccac ccgatccctg gagcggcttc agcactgctc gcccgcccag ctcgtcgacg
      961 aagtccttga tatcgcccac ggtgcgtgag atcagagttc ggggtcggat ctcttcgggg
     1021 aagtgctgga aataggtctt gttgatcgcg tcggcgagat ggaccgggtc attcaccacg
     1081 acgactcccg ccgcggtgac cagctggccg aacaggactc cggaggcctg ggcccagggc
     1141 cgctcgatgg cgtcttcggc cgggtcgttg cggagcatga ggatgtcgag ctcgtcgacc
     1201 gtgatcagct ccggatccac gtcttcgccc tgcacgtctt cgaggaagtt cttgagcgag
     1261 cgatacgtct tgtcgagggg gccgcgcgcc cgggccgtca cctcgcccga cgaagtgtgg
     1321 gcgaggtcac cgactcccat cagccatacc tcgtgcccgc gcttgtgagc ggccaaagcg
     1381 agccgggtgg tgctgtattc cggcttctcc gacatcacgt cgttgacgac cagacccaac
     1441 ttcatgcggc gacacctccc agctccagga tcccggcgcc atcctggatc ctggcgagtc
     1501 gatcgagtgc ctctcccgac tccaggaatc gaggtctgag tggcggctcg accaggacac
     1561 ctctttcgag gagatcggcc accagcggct cgtcggccaa ggatatcttc ccgaccagca
     1621 gtggatcgag ccggccaccc tcttggaggt aggtgaggag tcgggtcagc ccgccgagat
     1681 agagggcgtc cttggtcatc ccgccggacc gatgggcgcg catcgcagtg gtgaacgccc
     1741 gattccggcc cgagccgaga tcgatcaagg tgtcgtaggt ctgccggaag gtggcccgtc
     1801 cgctgaccga gtgcgccgcg accacccgga gcgcgaaggc acgcaaccgt ttcgggcgga
     1861 ggcccccgct gagatgctcg gccagcacac cgagcgcctc ctgggtctcc tcgtaccccg
     1921 ccagcccgcg tgagagcatc cgaatcggct gtgcgctccc gttgacgtag gtgaccacgt
     1981 ggatgccgat ctcgtgatgg agcaactgtt ccacccggtc gccggcgatc gaggtgtcga
     2041 cgccgatgaa caggtctccg ttctccacca tgaccccggc caccgtctcg ctgacatgga
     2101 ccagcgtcga gagctccggg aagcgccggc gatagtgctc gagctcgaca ttggcccttc
     2161 ccgccagttc atgggcgtcg acatagacgt ctgcggccgg ccccg
//
LOCUS       DASTXO010000476         2905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_249186, whole genome shotgun sequence.
ACCESSION   DASTXO010000476 DASTXO010000000
VERSION     DASTXO010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249186"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1920)
                     /locus_tag="VFU96_10085"
     CDS             complement(<1..1920)
                     /locus_tag="VFU96_10085"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU5113654.1"
                     /translation="MTKPLESIRRLPAVGVLAERQMGGFLAISALVGVGVGLGAAALI
                     YLVEWLEEFFLPFAIDQGHPWIFLTVPTGFLLAWFLARRFAPEVAGDGVPEAVAALEV
                     HSGRMRGRVIPLKILATAATVGTGGSAGREGPIVQIGAAVGSVISRRFNLGEDQVRSL
                     VAAGAGAGIGAAFNAPIAGMLFALEVILSSFAARHMSAIVIASITAAVTTRTIVGAEL
                     ELQTEAYRLQEFSELILYAGLALAVVLIGVLFLNGLGRLDSYLRPRARALGFLRPLLA
                     GLLVAGLIFLEPRLFGTGQAVINGLLAGDVGSSVWWALILLAVGKAVATSLTTTSGAS
                     GGAFMPSLFMGAAVGTGFALLVGEIWPSPIESGAFAVVGMAAMFAVVGRAPLTAILMV
                     FEVTGARDYQLILPLMLTATLGTFLAERFHPDSIYSMILDHMGINVRRTGEVDLLDTI
                     DVGSVMATQPHVCETGDSLAEVEERLHRTRSHGLPVVEGGRLVGIITLSDIARAGNGQ
                     DRVVDAMTHRPATVVQTTPVSTALERMAVLGVGRLPVVAEDDPEHLVGVFRREDAVRA
                     YHEALSANTDVELQRARLAQRTDPGAGYYEFRIPPGSMADGRSIREVAWPEGSTLVSI
                     RRDRDVQVPSGDTVLL"
     gene            1979..2809
                     /locus_tag="VFU96_10090"
     CDS             1979..2809
                     /locus_tag="VFU96_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113655.1"
                     /translation="MQFRSHVLRPIDPESPWNRAVFALAAAAGIIGAYLTLAEDAETL
                     LAIEAGGTAFLSWALARELDPDRQVPALVLSVVGGGWALLGPATAILPFTALLMAARL
                     VVETTGRRPLNTDLAGLALVATVVSFTALGWVMGFGLAVAIYVDDRMAEEPNRAALLA
                     SPAAAVGSSVVASFSGAFRTMPAVDPLVVAALGALALIAVLREPLEPVSFVDSRTKKF
                     IRRDRLQAGRVLSAVLVFFGAVVSGANAPAVVPMAIVIATALASTEVERLQRANPAPG
                     "
BASE COUNT          501 a          968 c          973 g          463 t
ORIGIN      
        1 cagcagaacc gtgtctccac ttggcacctg aacatctcga tcccgccgga tcgacaccag
       61 ggtcgacccc tcgggccagg cgacctcccg aatcgaccgg ccgtcggcca tcgagcccgg
      121 cgggatgcga aactcgtaat agcccgcccc cggatcagtg cgctgggcga ggcgtgcccg
      181 ctgcaactcc acgtcggtgt tggctgacaa ggcctcgtga taggcgcgca ccgcatcctc
      241 ccgcctgaag acccccacca agtgctccgg gtcatcctcg gctacgaccg ggagccggcc
      301 gacaccaagc accgccatcc gctccagggc ggtcgatacc ggcgttgtct gcaccacggt
      361 cgccggtcgg tgggtcattg cgtcgacaac acgatcctga ccgttgccgg cgcgggcgat
      421 atccgagagt gtgatgatcc ccaccagccg gccgccctcg accacgggta ggccatggga
      481 tcgggttcgg tgcagcctct cctcgacctc ggccagcgag tcgccggtct cacaaacatg
      541 aggctgggtg gccatgaccg agccgacgtc gatcgtgtcg aggaggtcga cctcgccggt
      601 ccttcgcacg ttgatcccca tgtggtcgag gatcatcgaa tagatggaat cgggatgaaa
      661 gcgctctgcc agaaacgtcc cgagggtcgc agtgagcatc agcggcagga tcaactgata
      721 gtcacgggcc cccgtgactt cgaaaaccat caggatggcg gtcagcggtg ccctgccgac
      781 gacggcaaac atggcggcca tcccgacgac ggcgaaggcg cccgactcga tgggagatgg
      841 ccagatctct cctacgagga gagcgaatcc tgttcccacg gctgcgccca tgaacagtga
      901 cggcatgaac gcgccgcctg atgcgcccga ggtggtcgtg aggctggtgg ccacagcctt
      961 gccgacagcg aggaggatca gggcccacca caccgatgag ccgacatcac cggcgagaag
     1021 accattgatg acagcctgtc cggtcccgaa caacctcggc tcgaggaaga tcagcccagc
     1081 gacgagcaga cctgcgagga gcggtcggag aaagcccaat gcccgggcgc ggggtcgtag
     1141 atagctgtcg agtcggccca gcccgttgag gaagagcaca ccgatgagga cgacggccag
     1201 ggccagaccc gcatagagga tgagctcgct gaactcttgg agtcggtagg cctccgtctg
     1261 aagctcgagt tcggcgccga cgatggtgcg ggtcgtcacc gccgcggtga tggaagcgat
     1321 gacgatggca gacatgtgcc gggcggcgaa cgaggagagg atgacctcga gagcgaacag
     1381 catgccggca atcggggcgt tgaatgcggc gccgatcccc gctcccgcgc cggctgccac
     1441 cagagacctc acctgatcct cgcccaggtt gaagcggcga gagatcaccg acccgacggc
     1501 ggccccgatc tggacgatcg ggccctcccg gcccgccgag ccacctgtgc cgaccgtggc
     1561 ggctgtggcg aggatcttca gcggaatgac ccggccgcgc atcctgccgc tgtgcacttc
     1621 gagggccgca accgcctctg gcacaccgtc tcccgcgacc tcgggtgcga atctgcgagc
     1681 gaggaaccaa gcgagcagaa agccggtggg tacggtcagg aagatccagg gatgtccctg
     1741 gtcgatggca aagggaagga agaactcctc aagccactcg accagataga tcagcgccgc
     1801 agcccccaag cccactccca caccgaccag ggcggatatg gcgaggaacc cacccatttg
     1861 tctctcggcg aggacaccga cggctgggag acggcggatg ctctccaacg gcttggtcac
     1921 gtggcgtcag ggtattcccc taccggcgtt tccctcgctc tcgtcacaga atgggccggt
     1981 gcagttccga tcccatgtgc tgcggcccat cgatccggag agcccgtgga accgggccgt
     2041 gttcgccctc gcggcggcgg ccgggatcat cggggcatac ctcaccctgg ctgaggatgc
     2101 ggagaccctc ttggcgatcg aggctggagg cacggccttc ctgtcctggg cgcttgcccg
     2161 ggaactcgat cccgatcgcc aggttcccgc cctggttctc tcggtggtgg gtgggggctg
     2221 ggccctgctc gggccggcga cggccatcct gccattcacc gcgctgctga tggcggcgag
     2281 gctcgtggtg gagaccacgg gccggcgacc cctcaatacc gatctggcag gtctggcctt
     2341 ggtggccacc gtggtctcct tcaccgcgct gggctgggtg atgggtttcg ggctggccgt
     2401 ggcgatatac gtcgacgacc ggatggctga agagcccaat cgggcggctc tcctcgcgtc
     2461 gccggccgcc gcggtgggtt cctcggtggt ggccagcttc agcggtgcct ttcggacgat
     2521 gcccgctgtt gacccgctcg tggtggcggc cctgggcgcg ctcgctctca ttgcggtgct
     2581 ccgggagccg ctcgagccgg tttctttcgt cgacagccgg accaagaagt tcatcaggcg
     2641 agaccggctt caggcgggtc gagtgctctc cgcggtcctc gtgttcttcg gggccgtggt
     2701 gtcaggagcg aacgcgcccg ccgtcgtgcc catggccatc gtcatcgcca cggctctcgc
     2761 ctcgaccgag gtggagcgtc tgcaacgggc aaacccggcc cccgggtagc gtggtccgca
     2821 tgtgcagatc gatcaaagtc ctcagggagg gcgccacacc ggctcccacc gatgagatcg
     2881 aggcggcagc gcttcagttc gtccg
//
LOCUS       DASTXO010000477         2614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1723592, whole genome shotgun sequence.
ACCESSION   DASTXO010000477 DASTXO010000000
VERSION     DASTXO010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1723592"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VFU96_10095"
     CDS             <1..665
                     /locus_tag="VFU96_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5113656.1"
                     /translation="NPEAAEETVRDVKAAGGEATSHEADVSSAGDSEAMVGAAESTYG
                     RLDVMFNNAGIMHSADDDAVSTDEGVWDLTMDINAKGVFLGCKYGIPALRRAGGGSII
                     NTASFVALLGAATPQVAYTASKGAVLAMTRELAVVHARENIRVNALCPGPLRTELLMK
                     FLDTDEKKQRRLVHIPMGRFGEAAEMAKAALWLASDESSYVTGADFVVDGGITAAYVT
                     PE"
     gene            662..2092
                     /locus_tag="VFU96_10100"
     CDS             662..2092
                     /locus_tag="VFU96_10100"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5113657.1"
                     /translation="MSTHHPFIDGEASPGTGGEVAIHDPGTGSEIGIVSRASSEDVDR
                     AVRAARRRFDEGVWRKATVRRRRDVLRGIADLIRRDQERLAALESSNAGKPIAAARGE
                     IGAVATTFDYYAGAVDKFHGQTIPGNADGTLMTFREPVGVCAAITPWNFPMLILSWKT
                     APALAMGNSIVVKPAEVTPLTALALAELAIEAGLPPGVFNVVTGKGSEAGEGLVRHPL
                     VSKISFTGSTEVGAGVMAAAAPDIKRVSLELGGKSASVVFADADLDTCVESSIFAVYD
                     NAGQDCCARSRVLVEEPVYEEFTSRFVERAGSLIVGDTADESTEMGPLITPAQRDSVE
                     GYIQVGVNEGAKLVCGGERVGTMGNFLSPAVFTGAESGMRIVQEEIFGPVVAVSQFNG
                     EDEAIALANDSRYGLSGSIWTRDIGRALRVARAVETGMLSINSSSSVHIEAPFGGVKA
                     SGVGREQGMAALDHYSEYKSVFIANA"
     gene            2113..>2614
                     /locus_tag="VFU96_10105"
     CDS             2113..>2614
                     /locus_tag="VFU96_10105"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEU5113658.1"
                     /translation="MKLGLLVCDHVRPEFEHISGDYPDMFRRLFAGHPEVELEVYDVI
                     GGESPKGPRECDAWITTGSRYSVNDDEPWIHHLEQFVREVAKEEVPFIGVCFGHQLIA
                     KALGGSVVKSERGWGVGAKEVEVREDLDLGPAYTVLTSHQDQIEDLPPGAEILGWNEH
                     CPVSMLG"
BASE COUNT          477 a          794 c          897 g          446 t
ORIGIN      
        1 tcaacccgga ggccgcggag gagaccgttc gggatgtgaa ggcagccggt ggcgaggcaa
       61 cctcccatga ggccgacgtt tcgtcggccg gcgacagcga agcgatggtg ggcgctgcgg
      121 agagcaccta cgggcgtctc gacgtgatgt tcaacaacgc cgggatcatg cactcggccg
      181 acgacgacgc ggtgtccacc gacgaagggg tctgggacct gaccatggac atcaacgcca
      241 aaggggtgtt cttgggttgc aagtacggga tcccggccct gcgccgcgct ggaggtggct
      301 cgatcatcaa caccgcctcg ttcgttgccc tgctcggggc ggccacaccg caggttgcgt
      361 ataccgcgtc caagggagcg gtgctggcga tgaccaggga gctggcggtt gtgcacgccc
      421 gggagaacat cagggtgaac gcactctgcc ccgggccgct gcggaccgag ttgttgatga
      481 agttcctcga caccgacgag aagaaacagc gccggctggt tcacatccct atgggccgct
      541 tcggcgaggc ggcggagatg gcgaaggcgg ccctgtggct ggccagcgac gagtcgtcct
      601 atgtgaccgg agccgacttc gtcgtagacg gtgggataac ggccgcatac gtgactccag
      661 agtgagcacg catcatccct tcatcgacgg ggaggcgagc cctggtaccg gaggagaggt
      721 cgcgatccat gatccgggaa ccgggagtga gatcgggatc gtcagtcgag cttcgtccga
      781 ggatgtcgat cgcgcggtgc gcgccgctcg gcgacgcttc gacgaggggg tgtggcgcaa
      841 ggcgacggtt cgccgtcgcc gcgatgtcct tcgaggcatc gccgatctga ttcgaaggga
      901 ccaggagcga ctcgcggccc ttgaatcgtc caatgccggg aagccgatcg ccgctgcccg
      961 gggagagatc ggcgccgtcg ccaccacatt cgactactac gccggggccg tcgacaaatt
     1021 ccacggccag accattcccg ggaacgccga cgggaccctg atgacattcc gggagcctgt
     1081 cggcgtatgc gccgccatca ctccgtggaa cttcccgatg ctgatcctca gctggaagac
     1141 ggccccggcc ctggcgatgg gcaactcgat agtcgtcaag cccgccgagg tgacaccgct
     1201 cacggcactg gctctggcgg agctcgccat agaagccgga ttgcctccgg gtgtgttcaa
     1261 tgtcgtcacc gggaaaggct ccgaggccgg cgagggcctg gttcggcacc cactggtgag
     1321 caagatctcc ttcaccggtt ccaccgaggt cggagcgggc gtgatggcgg cggctgctcc
     1381 cgatatcaag cgggtatcac tcgaactggg tgggaaatcg gcctcggtgg tgttcgccga
     1441 tgccgatctc gacacctgtg tggagtcctc gatcttcgcc gtctacgaca acgccggcca
     1501 ggattgctgt gcccgaagcc gagtcctcgt ggaagaaccg gtgtatgagg agttcaccag
     1561 ccgattcgtg gagcgcgcgg gatctctgat cgttggtgac accgccgacg agtcgaccga
     1621 gatggggccg ctgatcacac cggcgcaaag ggactccgtc gagggctaca tccaggtcgg
     1681 ggtcaacgaa ggagccaagc tggtctgtgg cggtgagagg gtcggcacca tgggcaactt
     1741 cctctcgccc gcagtgttca ccggggccga gtcgggcatg aggatcgtcc aggaggagat
     1801 cttcgggccg gtcgtcgcag tcagccagtt caacggcgag gacgaggcga tcgcgctggc
     1861 caacgactcg aggtacgggc tctccggatc gatctggacc cgggacatcg ggcgggcact
     1921 gcgggttgcg cgcgccgtcg agaccgggat gctctccatc aactcttcct cgagcgtcca
     1981 tatcgaggcg ccctttggcg gagtcaaggc atcgggtgtc ggacgcgagc aggggatggc
     2041 cgccctcgat cactactcgg agtacaagtc ggtcttcatc gccaacgcct gactgcgcgc
     2101 cagaatgaac gggtgaaact cggtcttctc gtctgcgatc acgtccgccc tgagttcgag
     2161 cacatctctg gcgactaccc ggacatgttt cgacgactct tcgccggtca ccccgaggtc
     2221 gaactcgagg tctacgacgt gatcggaggc gagtctccaa aaggtccacg cgagtgcgac
     2281 gcatggatca ccaccggctc ccgatattcg gtcaacgacg acgagccgtg gatccaccac
     2341 ctggaacagt tcgtacgcga ggtggcgaaa gaggaggtcc ccttcatcgg cgtctgcttc
     2401 ggtcatcaat tgatcgccaa ggcgctcggt ggcagcgtgg tcaagtccga acgcggctgg
     2461 ggggtggggg ccaaagaggt cgaggtccgg gaagatctcg acctgggccc cgcgtacacg
     2521 gtgctcacct cgcaccagga tcaaatagag gatctacccc cgggggcaga gatcctcggc
     2581 tggaatgagc actgtccggt ctcgatgctg ggtg
//
LOCUS       DASTXO010000478         5164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1686383, whole genome shotgun sequence.
ACCESSION   DASTXO010000478 DASTXO010000000
VERSION     DASTXO010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686383"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VFU96_10110"
     CDS             complement(<1..396)
                     /locus_tag="VFU96_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113659.1"
                     /translation="MKKYRNWLLLAVVMAIVIAACGGGDGEEATTTAGETATTGGETA
                     TTGGETATTAGEAPAGDTGYENLDAALAGEYEGTTVDVWAQWLPESSEASNFTYTLEP
                     FQEATGITVNFQPTPDYETALQVAVDGGNA"
     gene            complement(419..1375)
                     /locus_tag="VFU96_10115"
     CDS             complement(419..1375)
                     /locus_tag="VFU96_10115"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU5113660.1"
                     /translation="MTTTVGIDIGGSSTSAVALGPEAHIVGRHQMKAGVDGGRQMVSA
                     AVAAFAALEVDDCTAVGVGVPGQVDPRTGQVAMAVNLGVGAESYNLADELQAALGLPV
                     IIENDVHAAAVGAYESFRLEGCSPESLALVSVGTGIGAGVVVNGELLRGARGMAGEIG
                     HVVVDGSGVMCRCGQRGCLEAVAAGPAISRAWPKGVNGTAATALFGAASDGDPAAEKV
                     AGRIAGHLTTALIWLAATYDTEVIVLAGGVSGAGDPFLKAVREQVGRRGVASELAARR
                     LRPEQVVLARVDDPPGPRGAAVLAANHLLHTREAPAESKQKQ"
     gene            complement(1372..2550)
                     /locus_tag="VFU96_10120"
     CDS             complement(1372..2550)
                     /locus_tag="VFU96_10120"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU5113661.1"
                     /translation="MAVDSNQGDRRHTKATRHDSRRSNLRTTLELVSDQGATSRAEIA
                     RQTGLSRAAVSSLVGELIDHGLLRELGQGTSAGGKPPTLLALNERGRDIVAIDLGHRP
                     FRGALVDLSGRIHERVDADPAAYPPFGEKAHQVAVDLIETLISKSTAPVLGIGVGAPG
                     VISPEGTVLESTNLDWHGFDFGTELRHRFDIPVSIANDAAMAALFEFRRRPTDRNLIL
                     VKLGRGVGAGVVLDGTLHRGEHSAAGEIGHVRVRDGGDPCRCGNLGCLETVASVPAIL
                     RSLGADPERHPWDALALAGLVGEEPVRRALSEAGRHIGMVLANVVATLDIGHVVLASE
                     LLNASDILIEEIRNEMRSRILPATADLIEIEATQIGADLVLAGAASGVLVDRLGVVLR
                     "
     gene            2652..3854
                     /locus_tag="VFU96_10125"
     CDS             2652..3854
                     /locus_tag="VFU96_10125"
                     /inference="COORDINATES: protein motif:HMM:NF024622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HEU5113662.1"
                     /translation="MGTPHLLIRPGNPDFLDLPWDQPFNDWVHERLVEMPTGIHRHPV
                     VFVAYEEGVFVLKELPRRLANSEYQVFRALEERTTRSAEVVGLVERVWLDPHDEQSAA
                     VITRYVEHTFPYRHLVSGPGFGPRRDQMLDAIAGLLVELHLSGCFWGDCSLSNLLYRY
                     DAEAIESIMVDAETSRLYDRLSDGQRAEDLEIMKLNLAGEMADIAAMTGSEIDRADFT
                     LGEDVETRYRSLWDELTEELVITRDESYRIRERIGRLNQLGFSVNDVELTPTEEGSLV
                     RMKVEVGGRTFHSERLRQQTGIDASENQARVILSDLNYYLAKHGSVSASGKSVGTFKW
                     LTTSFEPMVARISELRRGNPVQGYCDLLHHRFRLAQNREQDVANEEALEDWVASGMPG
                     FDLAALPV"
     gene            3925..5154
                     /gene="ugpC"
                     /locus_tag="VFU96_10130"
     CDS             3925..5154
                     /gene="ugpC"
                     /locus_tag="VFU96_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017611920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEU5113663.1"
                     /translation="MARITMEGLGKIYEDGTRAVGDLDLAIEDGEFLVLVGPSGCGKS
                     TVLRMIAGLEDITEGTMMIGDKVVNDVAPKDRDIAMVFQNYALYPHMSVYDNMAFGLK
                     LRKFSKDEIQRRVEEAADILEIREYLQRKPKALSGGQRQRVAMGRAIVREPAAFLMDE
                     PLSNLDAKLRVQMRAELERLHSRLETTTVYVTHDQVEAMTLGDRVAVLRPVSSARPWN
                     LQQVGRPTDLFDHPDNLFVAGFIGSPAMNLSYGRLENIGDAVWVKLPNADLKVDARAL
                     EAHPGIENRMGEELVVGVRPNSLEAAAVKGPDPDRTLKAKVEVTEMLGAETYVHFTVN
                     RPPVVTPDIEELLADTGQDVSSLGDTTNFIARVSPDARVSRGDEIDLIVDCAKLHFFD
                     PKSGIRVGSEQKVGASV"
BASE COUNT          945 a         1653 c         1639 g          927 t
ORIGIN      
        1 ggcgttgcct ccgtcgaccg ctacctgaag cgccgtctca taatccgggg tgggctggaa
       61 gttgaccgtg atgccggttg cctcctggaa aggttccagg gtgtaggtga agttggaagc
      121 ttccgaggac tcggggagcc actgcgccca cacatccacc gtcgtacctt cgtactcccc
      181 ggcgagggcg gcgtcgaggt tctcgtaacc cgtatcgcct gccggcgctt cgccggcggt
      241 tgtcgccgtc tcgccgcccg tggtagccgt ttcgccaccg gtggtggctg tctcgccggc
      301 agtcgtggtg gcttcttcac cgtcgccgcc tccacaggcg gcaatcacga tcgccatcac
      361 cactgccagc aacagccagt tccggtattt cttcataccg ttcctccttc ttggttgctc
      421 attgtttctg cttgctttct gcgggagcct cccgcgtgtg aagaagatgg ttagcagcca
      481 gaacggctgc accgcggggc cccggcgggt catcgaccct agccaggacg acctgttcgg
      541 ggcgcagcct ccgggcggcg agctcggatg caacccctcg ccggccaacc tgttcgcgca
      601 ctgcctttag gaaggggtcg cctgcaccgg agacgcctcc ggccaggacg atcacctcgg
      661 tgtcgtaagt cgcggccagc caaatgagag cggtcgtgag gtgacctgca atccgaccgg
      721 ccactttctc ggcggcgggg tcaccgtccg aggcggcacc gaacagggca gtggccgcgg
      781 tcccattcac tcctttgggc catgcccttg aaatggccgg accggcggcg actgcttcga
      841 gacagccccg ctggccacat cggcacatga cacccgaccc atcgacgacg acatgcccga
      901 tctcaccggc catgcctcgt gctccgcgta acaattcacc gttcactacg acaccggcac
      961 cgattccggt tccgacgctg acaagtgcaa ggctctctgg gctgcacccc tcgagacgaa
     1021 aactctcata ggcgcctact gccgcggcgt gcacatcgtt ctcgatgatc accgggaggc
     1081 ccagcgccgc ctgaagctca tctgccaggt tgtacgactc ggccccaacg ccaagattca
     1141 ccgccatcgc cacctgaccc gtcctcgggt cgacctgacc gggcacgccc acgccgaccg
     1201 ccgtgcaatc gtcgacctct agcgcggcga aggcggcgac cgcggcggac accatctgcc
     1261 gaccaccatc gacaccggcc ttcatctgat gacgaccgac gatatgggct tcgggtccga
     1321 gggcaaccgc tgaagtagac gagccgccga tatcgattcc gaccgtcgtt gtcatcgcaa
     1381 caccactccg agtcggtcga cgaggacgcc cgaggcggcg ccggccagaa ctaggtccgc
     1441 tccgatctgg gtggcttcga tctcgatcaa gtcagcggtg gcagggagga ttcggctgcg
     1501 catctcgttg cgaatctcct cgatgaggat gtcgctggcg ttgagcagtt ccgaagccag
     1561 cacgacgtga ccgatgtcca gtgttgcaac gacgttggcc agaaccatcc cgatgtggcg
     1621 gccggcctcg gagagagccc gccgaaccgg ctcctcgccg acgagcccgg ccagcgcaag
     1681 agcgtcccag ggatgtcgtt cgggatccgc gccgagactt cgcaggatcg ccgggacgct
     1741 ggcgaccgtt tcgaggcagc caaggtttcc acagcggcaa gggtcgcccc catcccggac
     1801 gcgcacgtgt ccgatctcgc ctgcggccga atgctctccg cgatggaggg tcccatcgag
     1861 gacgactccc gcaccgacac cacggcccag cttgaccaag atgagattgc ggtcggtcgg
     1921 ccgtcggcga aactcgaaca gagctgccat cgcggcgtca tttgcgattg agaccgggat
     1981 gtcgaatcga tgccgcaact cggtgccgaa gtcgaagccg tgccagtcga ggttggtgga
     2041 ctccaggacc gtgccttcgg ggctgatgac gccaggggcc ccgactccga tcccgagaac
     2101 cggggcggtc gacttggaga tcaatgtctc gatcaggtcg acagccacct ggtgggcctt
     2161 ttcgccaaac ggtgggtagg ccgcgggatc cgcgtcgacg cgctcgtgga tgcgcccgct
     2221 caggtcgacc agggcgcccc ggaaggggcg gtgcccgaga tcgatggcca cgatgtcgcg
     2281 gccccgctcg ttcagggcga gcaaggtcgg gggtttccca cccgcggaag tcccttggcc
     2341 aagctcacgg agcagcccgt ggtcgatcag ctcgccgacg agactcgaga ccgcagctcg
     2401 ggagagaccg gtctgacgtg cgatttcggc cctactggtg gcaccttggt cggataccag
     2461 ctccagggtg gttcggaggt tgctgcgacg tgaatcgtgg cgggttgcct tggtgtgacg
     2521 gcggtcaccc tgattgctgt cgacagccaa cctttcctcc tccaaagaaa gttaagtaaa
     2581 cttacaaagt ccgaaggtcc cctgtcaaac acaatcgact gcttggtcga ggcggaccaa
     2641 aatgacctca catgggcacc ccacatctcc tgatccgtcc cgggaacccg gacttcctcg
     2701 atctgccctg ggatcagccg ttcaatgatt gggtccatga gcggctggtc gagatgccca
     2761 cgggcatcca tcggcatccg gtggtgttcg tcgcctacga ggaaggtgtg ttcgtactga
     2821 aggagctacc caggaggctc gccaatagcg aataccaggt cttccgggcc ctcgaggaac
     2881 gaacgacgag gtcggccgaa gtcgtaggtc tggtggagcg ggtatggctc gaccctcatg
     2941 acgagcagtc ggcagcagtc atcacccgat acgtcgagca caccttcccc taccggcacc
     3001 tggtcagtgg tcccggcttt ggaccgagac gcgaccagat gctcgacgcc atcgccggtc
     3061 ttctggtcga gctccatctc tcgggctgct tctgggggga ttgctcgctg tcgaacctgc
     3121 tctatagata cgacgccgag gcgatcgagt cgatcatggt cgatgccgag acgtcccgtc
     3181 tctacgaccg gctgtctgat gggcagaggg ctgaggacct ggagatcatg aagctgaacc
     3241 tcgccggtga gatggcggac atcgccgcca tgaccggatc ggagatcgac cgggcggatt
     3301 tcaccctcgg cgaggacgtc gagactcgct atcgatctct ttgggacgag ctcaccgagg
     3361 agttggtgat cacgagggac gagagctacc ggattcgcga acggataggt cgtctcaacc
     3421 aactcgggtt ctcggtcaac gatgtcgagc tcaccccgac cgaagaggga agtctggtca
     3481 ggatgaaggt cgaggttggc ggccgaactt ttcattccga gcgtctccgc cagcagactg
     3541 gcatcgacgc ctcagagaac caggcccggg tcatcctcag tgacttgaac tactacctcg
     3601 cgaagcacgg ttcggtgtcg gcgtcgggca agagtgtggg caccttcaag tggctcacga
     3661 cgtcgttcga gccgatggtc gcgaggatct cggagttgcg gaggggcaac ccggttcagg
     3721 gatactgtga tctcctgcat catcgatttc gcctggcgca gaaccgcgaa caagacgtgg
     3781 ccaacgagga agccttggag gattgggtcg cgtcgggcat gccgggcttc gatctggccg
     3841 cgttgcccgt ttgacttcgc cccaagtcag agaatttccg ctaggttcac gccggcggcc
     3901 gcgcgagatg agggagtaac aaagatggct cggatcacga tggagggtct cgggaagatc
     3961 tacgaggacg gcaccagggc cgtaggcgac ctcgacctcg cgatcgagga cggcgagttc
     4021 ctcgtgctgg tgggcccgtc tgggtgtggc aagtcgaccg tcctccggat gatcgccggt
     4081 ctcgaggaca tcactgaggg cacgatgatg atcggggaca aggtggtcaa cgatgtcgct
     4141 cccaaggacc gggacattgc catggtgttc cagaactacg ccctgtaccc ccacatgtcg
     4201 gtctacgaca acatggcttt cggtctcaag ctgaggaagt tctcgaagga cgagatacag
     4261 cgtcgggtcg aggaggccgc cgacatcctc gagatccgcg aatacctgca gcgcaaaccg
     4321 aaggcccttt cgggcggcca gaggcagcgt gtggccatgg gtcgagccat cgtgcgcgag
     4381 cccgccgcct tcctcatgga tgagcccctc tccaacctcg atgccaagtt gcgagtccaa
     4441 atgcgcgccg agttggagcg cctccacagc cgtctcgaga cgacgacggt gtacgtcacc
     4501 cacgaccagg tcgaggcgat gaccctcggt gaccgtgttg ccgtccttcg accggtttcc
     4561 tcggcgaggc cctggaacct gcaacaggtg ggccgtccga ccgatctctt cgaccatccc
     4621 gacaatctct tcgtcgccgg cttcatcggc agccccgcca tgaacctcag ctacggccgg
     4681 ctggagaaca tcggcgacgc ggtttgggtc aaacttccca acgccgacct gaaggtcgac
     4741 gccagggcgc tcgaggctca tccaggcatc gagaatcgga tgggtgagga gctggtagtg
     4801 ggggtccggc ccaattctct cgaggcggcc gccgtcaagg gcccagaccc ggatcggacg
     4861 ctgaaggcca aggtcgaggt gaccgagatg ctcggtgccg agacctatgt ccacttcacg
     4921 gtgaaccgtc ctccggtggt cacccccgat atcgaggaac tgctggccga caccggacag
     4981 gacgtttcct cactcggcga cacgacgaat ttcatcgcaa gggtgagccc ggatgcccgg
     5041 gtctcccgcg gtgacgagat cgacctgatc gtcgactgcg ccaagctcca cttcttcgac
     5101 ccgaagagcg ggatccgggt cggctcggag cagaaggtag gcgccagcgt ctgaccagca
     5161 ggct
//
LOCUS       DASTXO010000479         6163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_434365, whole genome shotgun sequence.
ACCESSION   DASTXO010000479 DASTXO010000000
VERSION     DASTXO010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434365"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VFU96_10135"
     CDS             <1..320
                     /locus_tag="VFU96_10135"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEU5113664.1"
                     /translation="FTHIGDLWDSEGPKGMDDDSMRDRENILLDLTARNDKVWGIFRV
                     VGTHTGSLYGIPATGRHIDVIEVGLWRVEDGVIAEAWYFGDELGLLRALEVNPPDLTK
                     ASD"
     gene            381..1121
                     /locus_tag="VFU96_10140"
     CDS             381..1121
                     /locus_tag="VFU96_10140"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU5113665.1"
                     /translation="MTTEAVLVILVIAGAYLIKGVAGMGGPLLAVPFIAGLASVELAV
                     VVLSLGNLVSNGWLLWEHRAGAKGTGFVMTPFLLVGIPATIAGTWLLTEVDDRILSIV
                     VAIVILGYIWRYLAAPHFVLERKTARRLAAPMGLIGGGLLGATGTAGPLLATYMHAIR
                     LGRSSFIHMMSLTFQVFGLIQVVALVVLGAFTPERLEQALWAIIPITVMTAVGIRIGR
                     RLNQRAFELVVLGLLGFAAVRLLLSAIG"
     gene            complement(1043..2470)
                     /locus_tag="VFU96_10145"
     CDS             complement(1043..2470)
                     /locus_tag="VFU96_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006089170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5113666.1"
                     /translation="MLSTSQQPVAARRAVISVRGVEKHFPIKEGTLQRVVGHVRAVDG
                     VSFEVRPGETMGLVGESGCGKTTLGRCISGLVPPTDGKIYFRLSDDDAARLDVIDAIP
                     EPDRTAEQRKDVERIDANHRVDVLSGKAWRTYRRNCQIVFQDSFSSLNPRQLVKDLVG
                     RPLQVHKEASGQELTERVVSLLESVGLGRQHLYRYPHQFSGGQRQRISIARALALDPE
                     FIVLDEPTSALDVSVQAQILNLLHELQKERGLTYLFISHDLGVVRLMSDRIAVMYLGK
                     VAEYGTSKELFEDPRHPYTEALLAANPDIASEESVMKGLKGTVPSPANPPEGCRFHTR
                     CPVATPACGWEVDDAVAWLQAHETLLAGLSDVHRRTNFDATLVFQDEDSASAVGRAFQ
                     DGDVPPAMKAALDNVSIEGRSVQISFFPVQPVWLEDIGDGHLTACLLHTQQGQEPWPP
                     QPIADSSNRTAANPSSPSTTNSNAR"
     gene            complement(2473..3687)
                     /locus_tag="VFU96_10150"
     CDS             complement(2473..3687)
                     /locus_tag="VFU96_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5113667.1"
                     /translation="MTQSQVGINTTQGQATRPLVASVRDLKVYFEAKAGVVHAVDGVS
                     FDIRDGETLGLVGETGCGKSVTARSFIRLVPSPPGIYAGGQIDFWSTESRRNKSEPID
                     LLQIPHKEMLEIRGDRIAMIFQDPGKALNPALSIRSQVAEVFYQHRSEEILESLGENV
                     PRLIRRAARQQSNSFERFLLNLPPWRAKAAKLRMRVDQLVAQALTETQIPNPRTIMDS
                     YPHELSGGMKQRVLIAQALACNPDLLIADEPTTALDVTIQARILELIAELQQRLETSV
                     LYISHDLSLVKEICDRVAVMYAGRIVETGTTEEVFSAPKHPYTRGLMAAVPSATHRRG
                     ELAAIEGTVPELVDPSPACRFNTRCPFAIEICRTVDPEVIAVGGEHTVACFAYYTGDG
                     SVALPTLERRVS"
     gene            3959..4570
                     /locus_tag="VFU96_10155"
     CDS             3959..4570
                     /locus_tag="VFU96_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEU5113668.1"
                     /translation="MRKLIVQTFVTLDGVMQAPGGPEEDPSGGFGYGGWLVNYWNEEA
                     DEVMGEATSKPFAMVLGRRTYDIMAAFWPYAPEEAGGKIFNEATKYVASRSQPALEWS
                     NSVLVEGDAADGLTALKAEDGPELVVYGSANLIQTLLSHDLVDEFRLWIFPLAIGSGK
                     RLFAEGTIPAGLQLVDSKISSTGVVMGTYRSAGEIITGTVGSD"
     gene            4612..5493
                     /locus_tag="VFU96_10160"
     CDS             4612..5493
                     /locus_tag="VFU96_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU5113669.1"
                     /translation="MRVATWNVNSLMARLEAVEKWLERAAPHVLLMQETKLADEDVPH
                     LILQAAGYEVAHHGEGRWNGVAIASRVGISEVGTNFGDGPVRDSRAAGLASEDFDPFD
                     QARMISATCAGIRFVSLYAPNGRQVGSPFFEGKLRWLARVHSWLAEEDLSPDEPMILG
                     GDLNVTPTDEDVWDAEKAHGGTHVSLEEREALARLREWGLVDAFRICRPEPGRFSWWD
                     YRAGMFQRNQGMRIDLLYVTRPVAERVVWAEIDREARKGKPTPSDHAPLVVDLDEPGK
                     PFDAGWEGAMARIATRK"
     gene            5571..>6163
                     /locus_tag="VFU96_10165"
     CDS             5571..>6163
                     /locus_tag="VFU96_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113670.1"
                     /translation="MKVVIRKVLCTLIPMVLLLVACGGGTDTATTTGPEAGGATSTSA
                     VVDQTTTTAAPTTTTTRAETTTTVAEQTAGIEDIPPECVAEISAILQVFEPAVEGIDW
                     ESATIDDHIQVMMSLASSSIGDTAACENVELDLTEEQGAALFLDVADQEAPGAAGYFA
                     AIMAMNEALDGRQSTGECQTDIATFEEVVAGGVPWVDL"
BASE COUNT         1102 a         1850 c         2048 g         1163 t
ORIGIN      
        1 gtttcacgca catcggcgac ctgtgggaca gcgaagggcc gaaggggatg gatgacgact
       61 cgatgaggga tcgagagaac atcctcctcg acctcactgc caggaacgac aaggtctggg
      121 gcatttttcg cgtggtcggt actcataccg gatcgctgta tgggatccct gcgacgggtc
      181 gccacataga cgtgatcgag gtcgggctct ggcgggtcga agacggggtc atagccgagg
      241 cctggtactt cggggacgag ctcgggcttc tccgtgccct cgaggtcaac ccgccagacc
      301 taacgaaagc atcggattag gtcggtcggc tgccgtatcc gcagcgatcc gagcgaccaa
      361 gcaactggtt tgcctgagcg atgaccaccg aggccgttct ggtcatcctg gtgattgccg
      421 gggcctattt gatcaagggt gtggccggca tgggagggcc actccttgct gtgcctttca
      481 tagccggcct cgccagtgtc gaactcgccg tggtggtgct gtcgctcggg aacctggtgt
      541 cgaacggctg gttgctatgg gagcatcggg cgggagccaa gggaacgggc ttcgtgatga
      601 ccccgtttct gctcgtaggc atcccggcga ccatcgcggg cacctggctc ctcaccgagg
      661 tggacgatcg aatcctgtcg attgtcgtcg ccatcgtcat cctcggctac atctggcgtt
      721 acctggccgc ccctcatttc gtactcgagc gaaaaacggc tcgtcgtctg gcagccccga
      781 tgggtctgat tggtggaggg ctcttgggtg ccaccgggac ggcaggccct ctccttgcca
      841 cctacatgca cgccataagg ctcgggcgct cctcgttcat ccacatgatg agcctcacct
      901 tccaggtctt cggactgatc caggtggtgg ccctcgtcgt cctcggcgcc ttcaccccgg
      961 agcggctcga gcaggccctt tgggccatca tccccatcac cgtgatgacc gctgtcggca
     1021 tccgcatcgg gaggagactc aatcagcggg cgttcgagtt ggttgtgctc gggctactcg
     1081 gatttgccgc ggtgcgattg ctgctgtcgg cgatcggttg aggaggccag ggctcctgcc
     1141 cctgctgtgt gtgaagcaaa catgcggtga gatggccgtc gccgatgtct tcgagccaga
     1201 caggctgcac ggggaagaag ctgatttgca cgctcctccc ctcgatggac acattgtcca
     1261 gtgccgcctt catggccggc gggacatctc cgtcctggaa ggcccggccc acagctgaag
     1321 ccgagtcctc gtcctggaac accaacgtgg cgtcgaagtt cgttcgacgg tggacgtcgc
     1381 tgagaccggc cagcaacgtc tcgtgggctt ggagccaggc aaccgcgtcg tcgacctccc
     1441 acccgcaggc gggggtggcc acggggcacc tggtgtggaa gcgacagcct tcgggcgggt
     1501 tggccgggct gggcaccgtc cccttgaggc ctttcatgac ggactcttcg gaggcaatgt
     1561 cagggttggc ggcgaggagt gcctcggtgt aggggtggcg ggggtcctcg aagagctcct
     1621 tggaggtgcc gtactcggcg acttttccca ggtacatgac ggcgatccga tcgctcatca
     1681 acctcacgac accgaggtcg tggctgatga acaagtaagt gaggccccgt tccttttgca
     1741 gctcgtggag caggttcagg atctgcgcct gcaccgacac gtcgagggcg ctggtcggct
     1801 cgtcgaggac gatgaattcc gggtcgaggg cgagagcgcg ggcgatcgag atccgctgtc
     1861 tttgcccccc ggagaactga tgcgggtacc ggtagaggtg ctggcggccg agtccgacgc
     1921 tctccaacag ggatacgaca cgctcggtca gctcctgacc cgaagcttcc ttgtggacct
     1981 gaagcgggcg gcccacgagg tccttgacga gctgtctcgg gttgagagag ctgaaggagt
     2041 cttgaaagac gatctgacag tttcggcggt acgttcgcca cgccttgccg gagagcacgt
     2101 cgacgcggtg gttcgcatcg attcgctcca catccttgcg ttgctcagcg gttcggtcgg
     2161 gctccgggat ggcgtcgatc acgtcgagcc tggccgcgtc atcgtcggag agcctgaaat
     2221 agatcttgcc atcggtcggc gggacgaggc cgctgataca ccggcccaga gtcgtcttcc
     2281 cgcatccgga ctcgccgacg aggcccatgg tctcgccggg acgcacctcg aaggagacac
     2341 cgtcgactgc ccggacgtgg cccacgaccc gttgcagggt cccctccttg atcgggaaat
     2401 gcttctcgac gccgcgcaca gatatcaccg cccgtcgagc cgccaccggt tgctgggagg
     2461 tgctcagcac ggtcacgaga cgcgcctctc cagggtgggc agggcaacgg agccgtcacc
     2521 ggtgtagtag gcgaagcagg caactgtgtg ctctccgcca actgcgatga cctctggatc
     2581 gacggtccgg caaatctcga tggcaaatgg gcatcgggtg ttgaatcggc acgccggcga
     2641 cgggtcgacc agctcgggca cggtcccctc gatggcggcg agctcgccac gtcggtgggt
     2701 ggcggaaggt accgcggcca tcagacctct ggtgtagggg tgcttgggtg cgctgaagac
     2761 ttcctcggtc gtccccgtct ccacgattcg accggcgtac atgaccgcga cgcgatcgca
     2821 tatctctttt accagcgaga gatcgtggct gatatagagg accgaggtct cgagacgctg
     2881 ttgtagctct gcgattagct ccaggatcct cgcctggatc gttacgtcga gggcggttgt
     2941 gggctcgtcc gcgatgagca ggtcgggatt gcaagcaagg gcttgagcaa tgagaacgcg
     3001 ttgtttcatc ccgccagaca gctcgtgggg gtagctgtcc atgatcgtgc ggggattggg
     3061 gatctgagtc tcggtgaggg cctgagctac gagctggtcg acgcgcattc gcagtttggc
     3121 cgccttggcc cgccacggag gaaggttcaa cagaaacctc tcgaagctgt tggactgctg
     3181 tcgagcggca cggcggatga gacgaggcac gttttcgccg agagactcga ggatctcctc
     3241 ggaacgatgc tgatagaaga cttcggcaac ctggctccgt atcgacaacg ccgggttgag
     3301 cgccttcccc gggtcctgga agatcatcgc gatccgatcg ccgcggatct ccagcatctc
     3361 cttgtggggg atttggagaa ggtcgatcgg ctcgctcttg tttcgacgtg actcggtgga
     3421 ccagaagtcg atctgtccgc cggcatagat ccctgggggg ctgggcacga gacggatgaa
     3481 cgagcgagcg gtgacactct tgccacagcc ggtctctccg accaacccga gagtctcccc
     3541 gtcacggatg tcgaaagaca cgccgtccac ggcgtgaacc acccccgctt tcgcctcgaa
     3601 gtagaccttc agatcgcgga ccgaggcaac caagggtcgc gtggcctgtc cctgtgtggt
     3661 gttgatgccg acctgactct gggtcacgag cccacccggt ccggccggtc cgaggcgccg
     3721 atcgaggtca cgccgtccag tatgtcatgg gatcgccggc tggctgactt caccctgtcc
     3781 tctcttgcgc aatcgattgc tgaccggaat cgcacgatat agcttgggcg ggtctgacgc
     3841 aacagcatgg gtcgctctta ccccggcccg gtggctcggt caccatcacc gatgagattg
     3901 gcagcgagac ccggtctaat actgatgagg ggaggcgccg gatcgaaagg acatcgtgat
     3961 gagaaagctg atcgtgcaga ctttcgtcac cctcgatggg gtcatgcagg ccccaggtgg
     4021 gcccgaggag gatcccagcg gcggttttgg ctacggcggc tggttggtca actattggaa
     4081 cgaggaagcg gatgaggtca tgggcgaagc gacgagcaag ccgttcgcca tggtcctcgg
     4141 acggaggacc tacgacatca tggccgcgtt ttggccctat gcccccgagg aggcgggcgg
     4201 aaagatcttc aacgaagcaa ccaaatacgt cgcgtcgcgg agtcaacccg cgctggaatg
     4261 gagcaactcg gttctcgtcg agggtgacgc cgccgatggt ctcaccgcac tcaaggcaga
     4321 agacggtccg gagctggtgg tctatggcag cgccaacctg atccagaccc tgttgagcca
     4381 cgacctcgtc gacgagttcc gcctttggat cttccccctc gccataggat cgggaaagcg
     4441 cctcttcgcc gagggcacca ttcccgccgg gctccagctc gtggacagca agatatccag
     4501 cacgggagtc gtgatgggca cctaccggtc ggccggggag atcatcaccg ggacggtcgg
     4561 atcggactga ctgctcgctc cgaaagcccg gatccgacca caattggtac cgtgcgcgtc
     4621 gccacctgga acgtcaactc gttgatggct cggctcgaag ccgtggagaa gtggttggaa
     4681 agggccgcgc cccacgtgct cctcatgcag gagaccaagt tggccgatga ggatgtccct
     4741 catctcatcc tccaggcggc cggctacgag gtagctcatc atggtgaggg acgttggaat
     4801 ggtgtggcca tcgcctctcg agtggggatc tcggaggtgg gcaccaactt cggtgacggc
     4861 ccggtacgcg acagtcgcgc cgcggggctc gcctctgagg acttcgaccc gttcgaccaa
     4921 gcccggatga tcagcgcgac ctgcgccggg atcaggttcg tcagcttgta cgctcccaac
     4981 ggtcgccagg tgggatcgcc gttcttcgag ggaaagctgc gatggttggc ccgcgttcat
     5041 agttggttgg ctgaggagga cctctctccc gatgagccga tgatcctcgg tggcgatctc
     5101 aacgtgaccc cgaccgacga ggacgtgtgg gatgcggaga aggcgcacgg agggacccat
     5161 gtctcactgg aggaacgcga ggcgctggca cgacttcgtg aatggggcct ggtcgacgca
     5221 tttcgcatct gtcgtcccga accgggccgc ttctcatggt gggactatcg agccggcatg
     5281 ttccagcgca atcagggaat gcggatcgat ttgctctacg tcacccggcc ggtcgcagag
     5341 cgagtggtat gggccgagat cgatcgcgaa gctcgcaagg gaaagcccac accgtccgac
     5401 catgccccat tggtggtcga tctcgacgag ccggggaagc cgttcgatgc cggctgggag
     5461 ggcgcgatgg cgaggatcgc cacccggaag tagcccgtcg atcccatcct gacccggatg
     5521 cggatcgcga tagtctcgga tcggacttgc acggagggag aggattcgtg atgaaagtcg
     5581 tcattcgcaa ggtcttgtgc acactgatcc ccatggtttt gctgttggtt gcgtgcgggg
     5641 gcgggacaga cacggccacg accactgggc ctgaagcggg tggggccacc tcgacatccg
     5701 ctgtcgtcga ccagaccact accacggcag ccccaaccac gaccaccacc agagcggaga
     5761 cgacgaccac agtcgccgaa caaacggcgg gcatcgagga cataccaccc gaatgcgtgg
     5821 ccgagatctc tgcgatcctc caagttttcg aaccggcggt cgagggcatc gactgggaga
     5881 gcgccaccat cgacgatcac atccaggtca tgatgtctct cgcctctagc tccattgggg
     5941 acactgcggc atgcgagaac gtagagctcg atctgaccga ggagcagggc gcagccctgt
     6001 tcctcgacgt cgcagaccag gaggcacccg gagccgcggg ttacttcgca gcgatcatgg
     6061 caatgaacga ggccctggat gggcggcagt cgactggcga gtgtcagacg gacatcgcca
     6121 ccttcgagga ggtggtcgct gggggagtgc cttgggtcga cct
//
LOCUS       DASTXO010000480         2616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_287093, whole genome shotgun sequence.
ACCESSION   DASTXO010000480 DASTXO010000000
VERSION     DASTXO010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287093"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VFU96_10170"
     CDS             complement(<1..373)
                     /locus_tag="VFU96_10170"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113671.1"
                     /translation="MKFALRLTTLGVIFTVMFSVIGLRLWFIQVAEGPQIARAAEEAT
                     WVGKTTYAARGDIYDRNGTLLATSRMVPAVMVDRTFVQPDQRDSLVQELASIVGVDPV
                     QLDDMYEEAGINGRFQVSTVSN"
     gene            complement(370..873)
                     /gene="mreD"
                     /locus_tag="VFU96_10175"
     CDS             complement(370..873)
                     /gene="mreD"
                     /locus_tag="VFU96_10175"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEU5113672.1"
                     /translation="MRGRTALVIGLTVVIAVVIQTTLFGRLRVVTPDLVMLIGIMLAL
                     TRVRTEAVLGVAFLSGLIMDLLGASLIGLRAVVYTTVAYIAIRTRERAELGRVTIALW
                     AGLLTLLGVVLLLLIGTLFGQAALLGEGAVSVMILVPLANVAVAALFAPTFVRLVDRD
                     RTALRYP"
     gene            complement(873..1802)
                     /gene="mreC"
                     /locus_tag="VFU96_10180"
     CDS             complement(873..1802)
                     /gene="mreC"
                     /locus_tag="VFU96_10180"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU5113673.1"
                     /translation="MLTSKQSDRALPTFITLLVIGVLLMTFDVRLEGGGVVAVLRSGT
                     QAIVSPMQRIAAYAVTPIANALDSLSNVASLREQNAALSNQLAESQAALVAVQDDLAK
                     LALLEQIYDLETDGAELGQTVANVIGSVDAALIIDKGTRNGVLAGQPVIDTNGYVIGT
                     VESASSGSATILPITASRDGLTVLVGSQTGTLVTRPFSSDLGLEMDLDVFDAREPVLV
                     GDRVLTSAASTDYPAGLPVGEVIEDASPESTSLTTTVRPFVDPEALRFVVVLAWPPDP
                     VSAATDDTIPEEVTDTTVAEEATSTTTTPEDDG"
     gene            complement(1825..>2616)
                     /locus_tag="VFU96_10185"
     CDS             complement(1825..>2616)
                     /locus_tag="VFU96_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEU5113674.1"
                     /translation="DVTEKMLRYFIQKVHRRKWARPRIVVCVPSGITPVERRAVEEAA
                     YHAGARRAYTIEEPMAAAIGSGLPIAEPAGSMVVDIGGGTTEVAVISLGGIVVSRSIR
                     VGGDELDTAIIDWVKKEYNVLIGERTAEQLKLAVGSAWPYADEPSAEVRGRDLVTGLP
                     KTILLSSPEIREAIEEPTQAVVDAVKFTLDKTPPELAADIVERGIVLTGGGALLVGLD
                     HRLAHETGMPIVTAANPLQSVVLGSGKCLEDFDTLQEVLVSSSRP"
BASE COUNT          486 a          828 c          841 g          461 t
ORIGIN      
        1 cgttggagac ggtgctcacc tggaagcgcc cgttgatccc ggcctcctcg tacatgtcat
       61 cgagttgaac tgggtcgacc ccgacgatcg aggccagctc ctggaccaag gaatctctct
      121 ggtcgggttg tacgaacgta cggtcgacca tgacggccgg gaccatccgg ctggtggcga
      181 ggagagtccc attccgatcg tagatgtcgc ctcgtgcggc gtatgtggtc ttgcccaccc
      241 aggtcgcctc ttcggcggcg cgggcgatct ggggtccctc cgcaacctgg atgaaccaga
      301 ggcgcaaccc gatcaccgag aacatcacgg tgaagatgac ccccagagtc gtgagtctca
      361 gcgcgaactt catgggtatc tgagagcggt ccggtcgcgg tcgaccaatc gtacgaaggt
      421 aggtgcgaag agggcggcaa ctgccacatt tgccagggga acgaggatca tgacgctaac
      481 ggcgccttct ccgagaagcg ccgcctgccc gaacaaggtc ccgatcagaa gcaggagcac
      541 cactcccagc agggtcagca gtccagccca caaggcgatg gtgactcgcc ccaactcggc
      601 tctctccctg gtcctgatgg cgatataggc cactgtggtg tacacgaccg cgcgcaggcc
      661 gatcagtgac gcgcccaaaa ggtccatgat cagcccggag aggaatgcga caccgagaac
      721 cgcctcggtc ctcaccctcg tcaacgccag catgatcccg atcagcatca ccaggtcggg
      781 agtgacgact ctgagcctcc cgaacaatgt ggtctggatc acgacggcga tgacgaccgt
      841 taggccgatc accagcgcgg tgcgtcctct catcaaccat cgtcctcagg ggtggtagtc
      901 gtcgaggtcg cctcctcggc gacggtcgta tcggtgacct cctcgggaat agtgtcgtcg
      961 gtcgctgccg acaccgggtc gggcggccat gccagaacga cgacgaaccg cagtgcctcg
     1021 gggtcgacga acggtcgcac ggtcgtcgtc aacgaggtcg actccgggga tgcgtcctcg
     1081 atgacctcac cgacggggag accggccgga tagtcggtgc tcgccgccga ggtgagaact
     1141 cgatcaccga cgagcaccgg ctcgcgggca tcgaagacgt cgagatccat ctccaaaccg
     1201 agatccgacg aaaagggcct cgtgacgagc gtcccggtct gtgagccgac gagaacggtg
     1261 agaccgtccc ggctcgcagt gatggggagg atggtcgcgc tccccgagct cgcactttca
     1321 acggtgccga tcacgtatcc attggtgtcg atgaccggct gtccggcgag gactccgttt
     1381 cgagtgccct tgtcgatgat gagggcggcg tcgaccgatc cgatgacgtt ggccacggtc
     1441 tgccctagct cggccccgtc ggtttccagg tcgtagatct gctcgagcag agccagcttg
     1501 gccaggtcgt cttgaactgc cacgagtgcg gcctgggatt cggcgagctg gttggacagg
     1561 gctgcgttct gctctctgag cgaggcgacg ttcgacaggc tgtcgagggc gttggcgatg
     1621 ggtgtgactg catacgcagc gatgcgttgc atcggtgaca cgatggcctg ggttccgctc
     1681 cgaagcacag cgacgactcc cccgccctcc aagcgcacat cgaaagtcat cagcaggacg
     1741 ccgatcacca agagtgtgat gaaggtcggg agtgctcggt cggattgctt cgaagtcagc
     1801 atgagtcaag acgggtcgag gtagtcatgg ccgggatgac gacacgagca cttcctgcaa
     1861 cgtgtcgaag tcctccaagc acttccccga gccgagcacc accgactgca gcggattggc
     1921 cgccgtgacg atgggcatac ccgtctcatg agccagacga tggtccagcc cgaccagcaa
     1981 agctccgccc ccggtgagca cgattccacg ctcgacgatg tcggccgcaa gctccggcgg
     2041 tgtcttgtcg agggtgaatt tgacggcatc gacgaccgct tgagtcggtt cctcgatggc
     2101 ctctctgatc tctgggctgg agaggagaat ggtcttgggc aggccggtga cgaggtcgcg
     2161 tccgcggacc tcggccgacg gctcatcggc gtatggccat gccgacccga ccgcgagctt
     2221 gagctgctct gcggtgcgct cgccaatcag gacgttgtac tccttcttga cccagtcgat
     2281 gatcgcggtg tccagctcgt cgcccccgac cctgatcgag cgggagacga cgatgccacc
     2341 aagggagatg accgcgacct cggtggtgcc gccaccgatg tccacgacca tggatcccgc
     2401 aggctcggcg atgggaagac ctgacccgat ggcggcggcc atcggctctt cgatggtgta
     2461 tgcccggcga gcgccggcgt gatacgccgc ctcttcgaca gccctgcgct ccaccggcgt
     2521 gatccccgat gggacacaca ccacgatccg aggacgcgcc cacttccgcc ggtggacctt
     2581 ctggatgaag tagcggagca tcttttccgt gacgtc
//
LOCUS       DASTXO010000481         2047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_913058, whole genome shotgun sequence.
ACCESSION   DASTXO010000481 DASTXO010000000
VERSION     DASTXO010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913058"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..597)
                     /locus_tag="VFU96_10190"
     CDS             complement(178..597)
                     /locus_tag="VFU96_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HEU5113675.1"
                     /translation="MIDAGFRALVAGFAVGDLVRLTEPLSLWGGLDPETGLIVDRNHP
                     ELGRSVTGSIIAMPHGRGSSSSSSVLAEALRLGTGPAGFVLESPDSILVIGSLVANRL
                     YGTTCPIVSGTLPEGSTGTWVIDHNHVAPLPGPGSGA"
     gene            complement(594..1832)
                     /locus_tag="VFU96_10195"
     CDS             complement(594..1832)
                     /locus_tag="VFU96_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HEU5113676.1"
                     /translation="MKLTESDQAMLHGDAGQAAKVAMELLVAVGESEDAPALLDISAS
                     HIDGGLYHGQAGLDFALSLVEGGARVVVPTTLNVSSLDLLHPQLYRGDEETAAAARRL
                     MDAYVDMGCTPSWTCAPYQLAQRPRLGDQIAWGESNAIVFANSVLGARTARYGDFLDI
                     CCALTGRAPAVGLHTEDGRWATLRVDLHVPDVLLDDDLLYPVLGHLLGQVAGTEVAVI
                     VGLDHRAGEDRLKALGAAAASSGAVAMFHATGVTPEAATLEDATGGHEPRRSVVVEID
                     DLTSARASLNHGEGPLGAISVGTPHFSARELATLATLVKGRSTRVPFYVNTSRDALSQ
                     AEAAGSNRAIEDFGATIVTDTCTYITPIMDDVTGVVMTNSAKWAFYAPGNLGVDVVFA
                     GLEACVASAVSGDLEIGGAW"
     gene            complement(1850..>2047)
                     /locus_tag="VFU96_10200"
     CDS             complement(1850..>2047)
                     /locus_tag="VFU96_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU5113677.1"
                     /translation="LADQYPGYNFEANKGYPCPVHKSALQAWGPSAIHRRSWVFMDYL
                     MWNGLERYHRPEPQMALPFED"
BASE COUNT          377 a          684 c          657 g          329 t
ORIGIN      
        1 ggccagggcg ggattggccg cgacaagctc gcccgtcgtc gacgtgcgat agaacgcgac
       61 cggcatccgc ttgaagaacg ccttcagctc gtcaaacccg ctcattcagg tattccatcg
      121 gcaaattggc gcgatttcta gacataccgc gattcacatg atcctcgcac ccgagaccta
      181 tgcccccgaa cctggccctg ggagaggggc gacatgattg tggtcgatca cccatgtccc
      241 tgtcgaaccc tctggaagcg tgccggagac gatcgggcag gtcgtcccgt acagacgatt
      301 ggcgaccagt gacccgatga cgagtatcga gtcgggcgac tcgagcacga agccggccgg
      361 gccggtcccg agccgcaggg cctccgccag caccgaggag gaggagctgg agccgcggcc
      421 gtgtggcatg gcaatgatcg agccggtcac acttcgcccg agttcgggat gattgcggtc
      481 gacgatcaga ccggtctcgg gatcgaggcc gccccacaac gacaacggtt cggtcaggcg
      541 caccaagtct cccactgcaa agcccgcgac cagggccctg aacccggcgt cgatcaccag
      601 gcacctccga tttccagatc tccggacacc gcagaggcga cgcaggcttc gagcccggcg
      661 aagacgacat cgacaccgag attccccggt gcgtagaaag cccatttggc cgagttggtc
      721 atgaccacgc cggtgacgtc atccatgatc ggggtgatgt aggtgcaggt gtcggtgacg
      781 atcgtggcgc cgaagtcttc gatggcgcgg ttcgagcccg ctgcctctgc ctgcgacaag
      841 gcgtcccgcg acgtgttcac atagaaggga accctcgtcg aacggccttt caccaatgtg
      901 gccagcgtcg ccagctctcg cgccgagaag tgcggggttc cgacactgat cgcccccagc
      961 ggtccttcgc catgattgag cgacgctcgc gccgaggtca ggtcgtcgat ctcgacgacg
     1021 acgctgcgtc gaggttcatg tccacctgtg gcgtcctcca gggtggccgc ctccggcgtg
     1081 actcccgtcg cgtggaacat ggctaccgca cccgaggagg cggccgccgc cccgagggcc
     1141 ttgagccggt cctctccggc cctgtgatcg agcccgacta tcaccgccac ctcggtccct
     1201 gccacctggc ccagcaggtg gccgagcacc gggtagagga ggtcgtcatc gaggagcacg
     1261 tcgggcacat ggagatcgac tcgcaacgtc gcccaccggc catcctcggt gtggaggccg
     1321 acggctgggg cgcggccggt cagggcgcaa caaatgtcga ggaagtcccc gtatcgcgcc
     1381 gtcctcgccc ccaggaccga gttggcgaag acgatggcat tcgactcacc ccaggcgatc
     1441 tgatccccaa gccggggacg ctgggcaagc tggtagggag cacatgtcca gctcggcgtg
     1501 cagcccatgt cgacgtaggc atccatcagg cggcgagccg ccgcggccgt ctcctcatca
     1561 ccccggtaca actgaggatg caacagatcg agtgaggaca cgttgagggt cgtcggcacc
     1621 accacccggg ccccgccttc caccagcgag agcgcgaagt cgagcccggc ctgcccgtga
     1681 tacaacccac cgtcgatgtg ggaggcggag atgtccaaga gtgccggcgc gtcttcggat
     1741 tcgccaaccg caacgaggag ctccatggcc accttcgccg cctgcccggc gtcaccatgg
     1801 agcatcgcct gatccgattc ggtgagcttc acgcgcgtac cgattccggt cagtcctcga
     1861 agggaagggc catctgtggc tcgggccggt ggtagcgctc caacccattc cacatgaggt
     1921 agtccatgaa gacccaggat cggcgatgga tggccgaggg cccccaggct tggagtgccg
     1981 acttgtgcac cgggcacgga taacccttgt tcgcctcgaa gttgtagccg ggatactgat
     2041 cggccag
//
LOCUS       DASTXO010000482         2206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1539384, whole genome shotgun sequence.
ACCESSION   DASTXO010000482 DASTXO010000000
VERSION     DASTXO010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539384"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VFU96_10205"
     CDS             <1..233
                     /locus_tag="VFU96_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113678.1"
                     /translation="EWDLTLAVWRLAARIGEDASAAFRIGYGAELPESRYRPWVAYHA
                     AMMMLEAAEVREGRLGDLGYLVDDLTEAVLGT"
     gene            330..536
                     /locus_tag="VFU96_10210"
     CDS             330..536
                     /locus_tag="VFU96_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113679.1"
                     /translation="MLEALVAVTATTGLFALAKGIRGNHQYRIDPRLVVPRDERGDDL
                     PCPWCYGPTYEEDTRCSGCGRSFG"
     gene            589..1566
                     /locus_tag="VFU96_10215"
     CDS             589..1566
                     /locus_tag="VFU96_10215"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU5113680.1"
                     /translation="MSRVGLVLGGGGITGAAYEMAALMSIRLATGWDPNEADVVVGTS
                     GGAFVTALVRNEALTLDSLVMPADDRDDVAERIRRHVFTKNGGVQVRHWVRHGIVPGL
                     RDPGLTLFLGSPAPYQATGLTDWVTSHIGEECSARWPAKPTAVVAFDIASRCRVVFGS
                     ESAPEAGLADAVAASSAIPLLFQPYPIDGRLYVDGGVVSGTHADVVLGSGQPLDLVLV
                     LAPMAADVSRKRARFHEKMFDRVGQRTLTEEIALIKGAWPDCDVVVLRPSPSVQNAMR
                     PNPMDSKRAVATFMRTLISMKRILAQPEVWDRLDDHLHAPARSRRIGVR"
     gene            1631..1987
                     /locus_tag="VFU96_10220"
     CDS             1631..1987
                     /locus_tag="VFU96_10220"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5113681.1"
                     /translation="MAVRPTHFEFYSADPRATVEFLTTVFGWRAEQWGDQDYWLIDTG
                     DGPGINGAVALAGDDGPKTLNTCDVEDLDAAIEAVEIGGGQLLTDVMEVPGVGRWVQV
                     EGPGGEEFGLMEPARD"
     gene            complement(1965..>2206)
                     /locus_tag="VFU96_10225"
     CDS             complement(1965..>2206)
                     /locus_tag="VFU96_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113682.1"
                     /translation="NHWIAHSLLVSDEALVEAQSWLWETCRVLAEPAACAPIAALTTG
                     AYLPATGEQVVAVISGANMALPDTWNEEINPGRAP"
BASE COUNT          375 a          690 c          737 g          404 t
ORIGIN      
        1 ccgaatggga tttgacgctc gccgtgtggc gtctggctgc ccgcatcgga gaagacgcct
       61 cggcggcatt ccgcatcgga tacggggcgg agctccccga gagtcgctat cgaccgtggg
      121 tcgcctatca cgcggcgatg atgatgctcg aggcagccga agtccgcgag ggccgtctgg
      181 gcgatctcgg ctatctcgtc gacgatctga ccgaagccgt cctcggtacg tgatacccag
      241 gggacgaccc gccccccacg tgcagccaaa tccacggttt tcgcgatctc ctctcttagc
      301 ctcaccacgc tgaagcaagg aggacggacg tgttggaagc actggtcgcg gtcacggcga
      361 cgacaggtct gttcgccctg gcgaagggga taaggggcaa ccatcaatac cggatcgacc
      421 cccggttggt tgtcccacgg gacgagcgag gcgacgacct gccctgccca tggtgttatg
      481 ggcctacata tgaggaggac acccgctgtt cgggctgtgg ccgttcattc ggttgaatga
      541 ggcgctagcc ccttagaaag agcccaccaa cccatttatg gtgtgaacgt gagcagggta
      601 ggactggtgc tcggcggtgg ggggatcact ggtgcggcct atgagatggc cgccctcatg
      661 tccatccgcc tcgccaccgg ctgggacccg aatgaggccg atgtagtcgt tggcacctca
      721 ggcggtgcgt tcgtcaccgc gttggtcaga aacgaggcgc tcacgctgga ttccctggtc
      781 atgcccgccg acgatcgcga tgacgtcgcc gagagaatcc ggagacacgt cttcaccaag
      841 aacggtggcg tgcaggtccg ccactgggta aggcacggaa tcgtccccgg tttgcgcgac
      901 cccgggctca cgctgttcct cggctcgccc gcgccgtacc aggccaccgg cctcaccgac
      961 tgggtgacca gccacatcgg cgaggaatgc tcggcccgct ggccggccaa gccgactgcc
     1021 gtcgtcgcct tcgatatcgc atctcgctgc cgggtcgtgt ttgggtccga atcggcgcct
     1081 gaggccgggc tggcagacgc cgtcgctgcc tcatcggcca ttcctctcct cttccagccg
     1141 tacccgatcg atggtcgtct gtacgtggat ggtggcgttg tctcggggac gcacgccgat
     1201 gtcgtgctcg ggtcggggca gccgttggac ctggtgctgg tccttgcccc gatggccgcc
     1261 gacgtctctc gcaagagggc acgctttcac gagaagatgt tcgacagggt cgggcaacga
     1321 acgctgacag aggagatcgc tctgatcaaa ggggcgtggc ccgactgcga tgtcgtcgtg
     1381 ctgcgcccgt cgccgtcagt ccagaacgca atgcgcccga acccgatgga ctcgaagagg
     1441 gcagtggcca cattcatgag aaccctgatc tcgatgaagc gcatcctcgc ccagcccgag
     1501 gtgtgggatc gactcgacga tcatctccat gcgccggctc gttcgaggcg catcggcgtg
     1561 cgctagaccc cgccggccgt ttcactcgcg atcggcccga ctactttgtc tgtcaaagag
     1621 aggagcctac ttggctgtca gacccaccca cttcgagttc tattcggcag atccccgagc
     1681 cacggtcgag tttctcacca ccgtgttcgg gtggcgggcc gagcagtggg gagatcagga
     1741 ctactggctg atcgacacgg gcgacgggcc cgggatcaac ggcgcggtgg ccctggccgg
     1801 tgatgatggc ccgaagaccc tcaacacgtg cgatgtcgag gacctggacg cggctatcga
     1861 ggcggtggag atcggaggcg gtcaactcct gacagacgtg atggaggttc ccggcgtggg
     1921 ccggtgggtg caagtagaag gtcctggcgg cgaagagttc ggcctcatgg agcccgcccg
     1981 ggattgattt cttcgttcca ggtgtcgggg agcgccatgt tcgctcccga gatgacggcg
     2041 acgacttgct cgccggtggc cggtagatag gcccccgtcg tcagcgcggc aatgggtgcg
     2101 caggctgcgg gctcggccag aacgcgacag gtctcccaca accaactctg ggcctcgacc
     2161 aaggcttcgt ccgatacgag cagcgagtgg gcgatccagt gatttg
//
LOCUS       DASTXO010000483         4711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_581616, whole genome shotgun sequence.
ACCESSION   DASTXO010000483 DASTXO010000000
VERSION     DASTXO010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581616"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..999)
                     /locus_tag="VFU96_10230"
     CDS             complement(16..999)
                     /locus_tag="VFU96_10230"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEU5113683.1"
                     /translation="MSARPGDGWRMVEMAGDLARQGVEFALATVVWRQAPSSGHQGAR
                     AIITADGEMHGWIGGACAEPVVIREAQRVIQDRQPRLIMLGAPDQFGEMPDGMMFVQM
                     SCQSEGALQLYIEPVLPSPRLVVVGRSPMAGTLVDLAAVLGWRADMIDAADFTSADVD
                     SRSVVVVATQGHGDEEAIEQAVRARPAFIGLVASRKRGETVLGYLADQGVPRELLDAV
                     RVPVGLDLGHTSHPEIAVAVLAELVELRANGALNPEPEKHHAHQGSSHPTGAPGTAID
                     LVCGMTVPADETSRPFEHEGQTYYFCCPGCRTAFEKDPITYIAKGTHAHQT"
     gene            complement(996..3350)
                     /locus_tag="VFU96_10235"
     CDS             complement(996..3350)
                     /locus_tag="VFU96_10235"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HEU5113684.1"
                     /translation="MGASRRRVEDNRFIRGKGNYIDDVVLPGMLHMEILRSPLAHARI
                     KSIDASKAWAMPGVRLVLTGETMAARNLAWMPTLSYDTQAVLATDKVRFQGQEVACVI
                     ADDPYIAKDACEAIVVDYEPLPAIVNPTQSLAEGAPLIRDDKENQPNNVVYDWEVGDK
                     DGTDRAFDQADLISTMDLHYPRSHPSPIECCGSIADFNRATSKLTVYLTTQAPHIIRA
                     AVALVAELPEQMIRIISPDIGGGFGNKVPVYPGYVASILASILLERPVKWIEDRTGNL
                     ISTGFARDIYLHGELALRKDGRILGVRMKATADHGAFFSDAQPSKFKIGLMHSAFACY
                     DIPSAHLEARGVYTNKAPGGVAYRCSFRVTEAMFFQERMVQAAADDLGMDQAEFRRVN
                     FVQDDDFPHRTPYGFLTDSGQYGKCLDVGLEAIGYEEFLKEKEEAAKRGRLLGIGIST
                     MTEPLGAGNSREYDILGIKMFDSAELKIHMTGKALLRTGAKTQGQGHETTWAQIVAHE
                     LGLPAEDVLVEEGDTDTAPFGMGTYASRSTPVAGAAVAMVSRKIRAKARKIAAHLLEI
                     SEEDVEWELGRFYVRSQPERGVTIQECAMAAYSNMPDGMEPGLETVAYYDPPNLTWPF
                     ACYIVTVEVDPQTGVWDVLRMVAVDDCGVRINPMIVEGQIMGGLTEAYAMANMQFITF
                     DEHGNCIGSNFTDYLLPTAWETPGFELHEVVTPCPHHPIGAKGIGECAAVGGPAAFVN
                     AVMDALKGTGVRNIDMPLFPDRVYEALQTGQDVSGAPPVRIGET"
     gene            complement(3406..3927)
                     /locus_tag="VFU96_10240"
     CDS             complement(3406..3927)
                     /locus_tag="VFU96_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU5113685.1"
                     /translation="MTSLFQEVSPGAAEDTPTRQITVTVNGDRRTAVVEPRLALAHLL
                     RQGFNLTGTHTGCDTSNCGACTVLFDGKPVKSCTMFAVQADGHEVMTVEGLAGPSELH
                     AIQEGFKEEHGLQCGFCTPGMMMASKALLDENPNPTEDEIRWALSGNLCRCTGYQNIV
                     KAVMWAAQKLESE"
     gene            complement(3929..>4711)
                     /locus_tag="VFU96_10245"
     CDS             complement(3929..>4711)
                     /locus_tag="VFU96_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEU5113686.1"
                     /translation="AGGQSLVPMIKLRFASPEMLVDINEIPGLDYHRVDPDGSIHVGA
                     LCRHADLERSSLLASRQPTMASAAPLVADPIVRNRGTLVGSLCHADPQGDWASVMVSL
                     DGLVVVEGHGGRRTIPVKDLVRGPFQTTLAYDEIVVEAIAPAPQGEPAGGYLKLERRV
                     GDFATVGVAVSIDRTGQEVTRAGIGLTGVGGSTISADDAAQVLVGGALTAEAIGEAAS
                     LSAQAAQPRSDHRGSASYKRHLVSVFTTRILTGLIRSEEEAA"
BASE COUNT          785 a         1554 c         1519 g          853 t
ORIGIN      
        1 accggtacct cgaagtcatg tttgatgagc atgtgtcccc ttggcgatgt aggtgatcgg
       61 gtccttctcg aaagccgtcc ggcagcccgg gcagcagaag tagtaggtct ggccttcgtg
      121 ttcgaagggg cgactggtct cgtcggccgg caccgtcatc ccacagacga ggtcaatggc
      181 agtgccggga gcaccagtcg gatgactcga gccctggtgg gcatggtgct tctcgggctc
      241 gggattgagc gccccgttgg ctcgcagctc gaccaactcg gcgagcacgg cgacagctat
      301 ctcgggatgg gacgtgtggc ccaggtcgag gccgaccggc accctcaccg cgtccaggag
      361 ctcacgcggg acgccctggt ccgccagata gccgagcacc gtctctccgc gcttccgcga
      421 ggccacgaga ccgatgaatg ccggccgcgc ccgtaccgcc tgctcgatcg cctcctcgtc
      481 accgtggccc tgggtggcga ccacgacgac cgatctggag tcgacgtcgg cgctggtgaa
      541 gtcggcggca tcgatcatgt cagctcgcca gcccaacact gcggccaggt cgacgagagt
      601 gccggccatg ggggagcggc cgaccacgac caggcgggga ctcgggagca cgggctcgat
      661 atagagctgg agcgccccct cggactggca tgacatctgc acgaacatca tgccgtcggg
      721 catctctccg aactgatcgg gagcgccgag catgatgagg cggggttggc ggtcctggat
      781 gacgcgctgt gcctcacgga tgaccacggg ctcggcgcac gccccaccga tccagccatg
      841 catctcaccg tctgcggtga tgatggcccg cgccccctga tggcccgatg atggcgcctg
      901 gcgccacacg accgtggcta gggcgaactc gactccctga cgggcgaggt cgccggccat
      961 ctccaccatc cgccacccgt cgccgggccg ggcgctcatg tctcgccgat cctcaccggc
     1021 ggagcaccgc tgacgtcctg tcccgtctgc agggcctcat agacacggtc cgggaacaac
     1081 ggcatgtcga tgttgcgcac acccgtgccc ttgagggcgt ccatcacggc attgacgaat
     1141 gcggccggcc caccaaccgc ggcgcattca ccgattccct tggctccgat ggggtggtgg
     1201 gggcacgggg tgaccacctc gtggagctcg aatcccggcg tctcccatgc ggtcgggagc
     1261 aaatagtcgg tgaaattgga gccgatgcag tttccgtgct cgtcgaaggt gatgaattgc
     1321 atgttcgcca tggcgtaggc ctcggtgagc ccgcccatga tctggccctc gacgatcatc
     1381 gggttgattc gcaccccgca gtcgtcgacg gccaccatgc gcagcacgtc ccacacccct
     1441 gtctgcgggt ccacctccac tgtgacgatg tagcaggcga acggccaggt gaggttgggc
     1501 gggtcgtagt acgccacggt ctcgaggccc ggctccatcc cgtcgggcat gttggagtac
     1561 gccgccatgg cgcactcctg gatggtgacc cctcgctctg gctgactgcg tacgtagaag
     1621 cggcccagct cccactcgac atcctcttcg gatatctcga ggaggtgtgc cgcgatcttt
     1681 cgagccttcg cccggatctt gcgcgacacc atggcgaccg ccgcccccgc cacgggtgtg
     1741 cttcgggagg catacgtccc catcccgaag ggggccgtgt cggtgtcacc ctcttctacg
     1801 aggacgtcct cggcggggag gccgagctcg tgagcgacga tctgggccca ggtggtctcg
     1861 tggccctgac cctgggtctt ggccccggtc cgaagcagag ccttgccggt catgtggatc
     1921 ttgagctcgg ccgaatcgaa catcttgatg cccaagatgt cgtactcacg actgttgccc
     1981 gccccgaggg gctcggtcat cgtggagatg ccgatgccga gcagccggcc tcgtttcgcc
     2041 gcttcttcct tttccttcaa gaactcctcg tagccgatgg cctcgagacc gacgtcgagg
     2101 cacttgccgt actggcccga gtcggtgagg aagccatacg gggtccgatg ggggaaatcg
     2161 tcatcttgca cgaaattgac ccggcggaac tcggcctggt ccatccccag gtcgtccgcg
     2221 gccgcctgca ccatccgctc ctggaagaac atggcctcgg tgacgcggaa cgagcatcga
     2281 taggcgacac cgccgggtgc cttgttggtg tacacccctc gtgcctcgag atgggcagaa
     2341 gggatgtcgt aacaggcgaa cgccgagtgc atgagcccga tcttgaactt cgacggctgg
     2401 gcgtctgaga agaaggcgcc gtggtccgcc gtcgccttca tccgcacgcc gaggatccgc
     2461 ccgtcctttc gcagggccag ctcgccgtgg agatatatgt ctcgggcgaa accggtcgag
     2521 ataaggttgc cggttcggtc ctcgatccac ttcaccggcc gctcgagcag gatcgaagca
     2581 aggatcgaag ccacataccc cgggtacacc ggcaccttgt tgccgaaccc gcccccgatg
     2641 tcaggcgaga tgatgcgaat catctgttcg ggcagctcgg ccaccaatgc cacggcggca
     2701 cggatgatgt ggggtgcctg agtggtcaga tagaccgtca gcttggaggt tgctcgattg
     2761 aagtcggcga tcgagccgca gcattcgatc ggagatgggt gggacctcgg gtaatggagg
     2821 tccatcgtgg atatgagatc ggcttgatcg aaggcgcggt cagtgccgtc cttgtcgccc
     2881 acctcccagt cgtagaccac gttgttcggc tggttttcct tgtcgtcccg gatcagggga
     2941 gcgccctcgg ccagtgactg ggtcgggttg acgatcgcgg ggagcggctc gtagtcgacc
     3001 acgatcgcct cgcaggcatc cttggcgatg taggggtcat cggcgatcac acaggcgacc
     3061 tcctggccct ggaaccgcac cttgtcggtg gccagcaccg cctgggtgtc ataggagagc
     3121 gtcggcatcc acgccaggtt gcgagcggcc atagtctcgc cggtcagcac gaggcggacc
     3181 ccgggcatcg cccacgcctt cgaggcgtcg atcgacttga tcctggcgtg agccaggggg
     3241 ctgcgcagga tctccatgtg gagcatcccc ggaagcacaa cgtcgtctat gtagttgccc
     3301 ttgccgcgga tgaagcggtt gtcttcgaca cgacgcctgc tggcgcccat gccgccgatc
     3361 ttgatctcgt cgaccgccgt cacctgatca cctccgttga ccgcatcact cgctctccag
     3421 cttctgggcc gcccacatga ccgccttgac gatgttctga tagccggtgc atcggcacag
     3481 attgccgctc aatgcccacc ggatctcgtc ctcggtcggg ttcgggttct catcgaggag
     3541 agctttggag gccatcatca tgccgggagt acagaagccg cattggagcc catgctcctc
     3601 cttgaacccc tcttggatcg cgtgcagctc gctggggccg gccaggcctt cgactgtcat
     3661 cacttcgtga ccgtcggcct ggacggcgaa catggtgcag ctcttcaccg gcttgccgtc
     3721 gaagagaacc gtgcacgccc cacaattcga cgtgtcgcag ccggtgtggg tgccggtcag
     3781 gttgaaaccc tgacgaagga gatgggcgag ggccagtctc ggctcgacaa cggcggtccg
     3841 acggtccccg ttcacagtca cggtgatctg tctcgtcgga gtgtcctccg cggctcccgg
     3901 gctgacttcc tggaacaagc tcgtcatctc aggccgcctc ctcttccgat cgaatcaacc
     3961 cggtcaggat cctggtcgtg aacacactca ccaggtggcg cttgtacgag gcactgcccc
     4021 tgtggtcact gcggggctgg gcggcctggg cggacaatga cgccgcttca ccgatggctt
     4081 ccgcagtcag cgcccctccg accaggacct gggcggcatc gtcggccgaa atggtcgagc
     4141 caccaacacc ggtgaggccg atcccagctc gggtcacctc ctgcccggtt cggtcgatcg
     4201 aaaccgcgac ccccacagtc gcgaaatcgc cgacccggcg ctccagcttc agatacccgc
     4261 cggcaggctc gccctgcgga gcgggggcga tcgcttcgac gacgatctcg tcataggcaa
     4321 gagtggtctg gaacgggccg cggacgaggt ccttgaccgg gatcgttcgc cggccaccgt
     4381 ggccttcgac cacgaccagg ccgtcgagcg agaccatcac cgacgcccag tcgccctggg
     4441 gatcggcatg acagagcgat ccgacgagcg tccctcgatt cctcacgatc gggtcggcca
     4501 ccaaaggcgc cgccgaagcc atggtgggtt gccggcttgc caggagcgac gagcgctcga
     4561 ggtcggcatg acggcacaag gccccgacgt ggatcgaccc atcaggatcg acccggtgat
     4621 aatcgaggcc ggggatctcg ttgatgtcga caagcatctc cggcgatgcg aagcgcagtt
     4681 tgatcatcgg gaccaggctc tggccaccgg c
//
LOCUS       DASTXO010000484         2105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_581669, whole genome shotgun sequence.
ACCESSION   DASTXO010000484 DASTXO010000000
VERSION     DASTXO010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581669"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /gene="dctP"
                     /locus_tag="VFU96_10250"
     CDS             complement(<1..405)
                     /gene="dctP"
                     /locus_tag="VFU96_10250"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEU5113687.1"
                     /translation="MSLRKLWIGGYGPRESAHGDGLDAFRRAVADGTNGEVEVEVTWN
                     IMEEGRPNTDLFDLVEAGEMFMCYFSSSYLGDRVPELNVLETPFLFTDLAAAHRALDG
                     ELGRALARAVRARSGFEVLGFWDNGFRHMTNRL"
     gene            512..1744
                     /locus_tag="VFU96_10255"
     CDS             512..1744
                     /locus_tag="VFU96_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxyltransferase"
                     /protein_id="HEU5113688.1"
                     /translation="MDREPWKSDMWFSSPWNYLPEVTEGFSIPSDVRVHDVTLRDGEQ
                     QAGVEFTSDDKVRIATALAEAGIHRIEAGLPAVSPSDADAIKRMVAEGLPTEIYAFSR
                     CMVPDVELALDCGVKGVVMEVPASHHLIEKAYRWPVEKAIEASVEATKYAHENGLKVT
                     FFPIDATRATMTELLDDLEQVATEGHVDNIALVDTFGVTSPHAIRYYTQRVRERMAVP
                     LETHFHMDFGMGVANTALAVAEGASVIHTTVSGIGERAGNAPTEETVLALLTMYGIDT
                     GIDTTKFTEIARMVADLSGVTQPANRPVTGEMLYNVESGIIATWVKNVGDELLEPFPF
                     RPELVGQADPKIVLGKGSGLDSVAIWLGAHGIHDAETKEIEAILAEVKGRSLAKKGLL
                     DDDEFLEIVREVIPDKVD"
     gene            1774..>2105
                     /locus_tag="VFU96_10260"
     CDS             1774..>2105
                     /locus_tag="VFU96_10260"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEU5113689.1"
                     /translation="MREAFFGLSTDWKEGINWANVRDWRLKRAHAAMERHGLGALLLM
                     YDENMRYVSSTLTPGWNRLKPGLRYVVLTAGKPPIVYEQGDIGFHLEVHNPWIPKENI
                     RYSYAWIKG"
BASE COUNT          428 a          683 c          640 g          354 t
ORIGIN      
        1 gagccgattg gtcatgtgcc ggaatccgtt gtcccagaag ccgagcactt cgaagccgct
       61 cctggcccgc accgctcggg cgagagcccg accgagctca ccgtccaggg cacgatgggc
      121 agcggcgagg tcggtgaaca agaacggcgt ctcgagcaca ttcagctccg gtacccggtc
      181 gccgagatag ctggaggaga aatagcacat gaacatctcc ccggcctcga cgagatcgaa
      241 caggtcggtg ttgggccgtc cctcctccat gatgttccag gtgacttcga cctcgacctc
      301 gccgttggtc ccgtctgcaa cggcccgtct gaaggcgtcg agaccgtcgc catgagcgct
      361 ctcccgtggc ccgtagccac cgatccacag ctttctcagg ctcatttcgg gtttcgggac
      421 gggccgacgg gtcgatcctg tttgctctgg gcgatcatcg atcgtatatg atgcaggatc
      481 gtcctgtcga acgggtgagg agcagagaaa catggatcga gagccttgga agagcgacat
      541 gtggttctcg agcccctgga actacctccc cgaggtgact gagggattct cgatcccgtc
      601 cgatgtccgg gtacacgacg tgaccctccg cgatggggaa cagcaggccg gagtcgagtt
      661 cacctccgac gacaaggtga ggatcgccac cgccttggcc gaagcgggca tccatcgcat
      721 cgaagccggt ctccccgccg tctccccttc cgacgccgat gccatcaagc gcatggtggc
      781 cgaaggtttg cccactgaga tatacgcctt cagccgatgc atggtccccg acgtcgagct
      841 cgcactggat tgcggggtca aaggggtggt gatggaggtg cccgccagcc accacctcat
      901 cgagaaggcc tatcgctggc ccgtggagaa agcgatcgaa gcctcggtcg aagccaccaa
      961 atacgcccac gaaaacgggt tgaaggtgac cttcttcccc atcgacgcca cccgggccac
     1021 gatgaccgag cttctcgacg atctggagca ggtcgccacc gagggccacg tcgacaacat
     1081 cgcgctcgtc gacactttcg gtgtcacctc cccgcacgcc attcgctact acacccaaag
     1141 ggttcgggag aggatggcgg ttcccctcga gacccatttc cacatggact tcgggatggg
     1201 cgtggccaac accgcgctgg ccgtcgccga aggcgcatcc gtgatccaca ccaccgtcag
     1261 cggcatcgga gaacgggccg ggaacgcgcc caccgaggag accgtcctcg ccctgctcac
     1321 catgtacggc atcgacaccg ggatcgacac caccaagttc accgagattg cccgaatggt
     1381 cgccgacctg tccggagtca cccagcccgc caaccggccg gtgaccggcg aaatgctcta
     1441 caacgtcgag tccggaatca tcgccacctg ggtgaagaac gtcggcgacg agctactcga
     1501 gcccttcccc ttccgccccg agttggtcgg ccaggccgat cccaagatcg tgctcggcaa
     1561 aggctcaggc ctcgactcgg tcgcgatctg gctgggagct cacgggatcc acgatgccga
     1621 gacgaaggag atcgaggcga tcctggccga ggtcaaaggc cgttcactgg cgaagaaagg
     1681 gctcctcgac gacgacgagt tcctggaaat cgtccgggaa gtgatcccgg ataaggtcga
     1741 ctgaccaagc aaaccacgga ggcatgatca gggatgagag aagcgttctt cgggctgagc
     1801 accgactgga aagagggcat caactgggcc aatgtccgtg actggaggct gaagcgggcc
     1861 catgccgcca tggaacgcca cggcctcgga gccctcctcc tcatgtacga cgagaacatg
     1921 aggtatgtgt cctcgaccct gacgccgggt tggaaccggc tcaagccggg tctccgctac
     1981 gtcgtcctca ccgccgggaa gccccccatc gtctatgagc agggagacat cggctttcac
     2041 ctcgaggtgc acaatccgtg gatcccgaag gagaacattc gctactccta tgcgtggatc
     2101 aaggg
//
LOCUS       DASTXO010000485         2816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_29240, whole genome shotgun sequence.
ACCESSION   DASTXO010000485 DASTXO010000000
VERSION     DASTXO010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29240"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="VFU96_10265"
     CDS             <1..794
                     /locus_tag="VFU96_10265"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HEU5113690.1"
                     /translation="VDGIEGDTPDVGFDRNGVAHGIACDFVIGCDGAHGVSRDFIPAA
                     GRNSYERHYPFCWVGLLSETPPVDHELIYASHERGFALCSMRNEHRSRYYIQGPSDTT
                     LEDWPDDRFWSELTARLPGPVVDNLITGPSIEKIVTPVRSWVSEPMRHGRLLLAGDAA
                     HVVPPTGAKGLNLAISDVVYLVDAIAGHSDNGSMTGLDEYSERALARVWKAVRFSWWM
                     TTMLHRFPGRGDDFDRRLQEAELSYLFASENAQRTMAENYVGLPH"
     gene            820..1188
                     /gene="pcaC"
                     /locus_tag="VFU96_10270"
     CDS             820..1188
                     /gene="pcaC"
                     /locus_tag="VFU96_10270"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HEU5113691.1"
                     /translation="MGSYEDGMKVRRAVLGDEYVDAATESVTDLDADFQRWITESAWG
                     GVWARPDLDLRTRSLLTIAILGALNHDELELHLRAARNTGVTPDEVREALLHVAVYAG
                     VPAANRAFKVAKDVYADEDL"
     gene            1185..1910
                     /gene="pcaH"
                     /locus_tag="VFU96_10275"
     CDS             1185..1910
                     /gene="pcaH"
                     /locus_tag="VFU96_10275"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HEU5113692.1"
                     /translation="MSEPVIRHYGPKAYADHPPFSYPDYRSTVKRSPTHDLIRIVQTL
                     SETTGPGPAWTEISEADTDLTTNTGTGSPAIGERIIVTGKVLDEEGRGLPDTVVEIWQ
                     PNASGRYAHWRETEFPAPLDPNFVGVGQCLTDAEGGYRFTTIKPGPYPWGNHPNAWRP
                     SHIHFSIMGPSLGMRLVTQMYFPGDPLIPLDPIFNSVPAHARSRMIATYDHDVTEANW
                     ALGYRWDIVLRGEQATPHDEGGD"
     gene            1907..2488
                     /gene="pcaG"
                     /locus_tag="VFU96_10280"
     CDS             1907..2488
                     /gene="pcaG"
                     /locus_tag="VFU96_10280"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HEU5113693.1"
                     /translation="MSQLLGQTPSQTVGPFYTLRLFREGENILVPPGHTGRIVIQGRV
                     LDGDRSPIEDGLLEIWQANSVGRYHHPDDRRDLALDNGFTGFGRAITDFETGGYTFET
                     LKPGPVPDVEGASQAPHISLIVQGRGMLNPVFTRIYFSDEKEANRDDLILRSVPAERR
                     GSLIAELVDDGEPRVYRFDVLFQGDDETVFFDF"
     gene            2488..>2816
                     /locus_tag="VFU96_10285"
     CDS             2488..>2816
                     /locus_tag="VFU96_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HEU5113694.1"
                     /translation="MLDPGFTTEEMAMIFTPAHWVEAVARFEAALALALADAGIAPRE
                     DAEAVAEAWQTLVIDPDDVLAATWSAGTPMTPLLEEIRGRLPEERAKWAHHGSTTQDA
                     VDTATMI"
BASE COUNT          551 a          919 c          866 g          480 t
ORIGIN      
        1 gtgtcgacgg catcgaaggc gacacccccg atgtgggatt cgaccgcaac ggtgtggcgc
       61 acggcatcgc ttgtgacttc gtaatcggat gcgacggcgc gcacggggtg agtcgggact
      121 tcatccccgc cgccggcaga aattcctatg agcggcacta cccgttctgt tgggtagggc
      181 tgctttccga gacccccccg gtggaccacg agctgatcta cgccagccac gaacgcggct
      241 ttgccctctg ctcgatgcga aacgagcatc gcagtcgtta ctacatccag ggcccatcgg
      301 acacgacact cgaagactgg cctgacgacc gtttctggtc tgagttgacg gcgagacttc
      361 ccggcccggt ggtcgacaac ctaatcacag ggccttcgat cgagaagatc gtcacacctg
      421 tgcgaagctg ggtttccgag ccaatgcggc atgggagact gcttctggcc ggggacgctg
      481 cccatgtcgt ccccccaacc ggggccaagg gcctcaatct cgccatttcc gacgtggtgt
      541 atctcgtcga tgccatcgcc ggccactccg acaacggctc catgaccggt ctcgacgagt
      601 attcggagag ggcactggcg cgagtgtgga aagccgtgcg attctcctgg tggatgacga
      661 ccatgctcca ccgtttcccg ggaaggggcg atgacttcga ccggcgactc caagaagccg
      721 agttgagcta tctgttcgcc tcggagaacg cacaacggac gatggccgag aattacgtcg
      781 ggcttcccca ctgaacaggg cgacgagcga ggattgctta tgggttcgta cgaagacggg
      841 atgaaagtgc gaagagccgt gcttggcgac gagtacgtcg acgccgcgac cgagtcggtg
      901 accgatctcg acgccgattt tcaacgttgg atcaccgaga gcgcatgggg tggggtatgg
      961 gcccggcccg acctcgacct gagaacccgg agcctgctca ccattgccat cctcggtgcc
     1021 ctcaaccacg acgagctcga gcttcacctc cgtgccgctc gcaacacggg agtcaccccc
     1081 gacgaggtgc gagaggctct tctccatgta gcggtctacg ccggagtccc cgcggccaac
     1141 cgggcgttca aggtcgccaa ggacgtctat gcggatgagg acctgtgagc gagccggtca
     1201 tcaggcacta cgggccgaag gcatacgccg atcatcctcc cttctcctac cccgactacc
     1261 ggtcgacggt gaagcgaagc ccgacccacg acctgatccg gatcgtccag acgctctccg
     1321 aaaccaccgg cccggggccg gcgtggacag agatctctga ggccgacacc gatctcacca
     1381 cgaacaccgg gaccgggagc cccgcaatcg gcgagcggat catcgtcacc gggaaggtgc
     1441 tcgacgaaga agggcgaggg ttgcccgaca ccgtcgtaga gatatggcag ccgaacgcca
     1501 gtggccgata cgcccactgg cgtgaaaccg agtttcccgc cccgctcgac cccaatttcg
     1561 tcggtgtggg ccagtgcctc acagacgctg aaggtggata ccggttcacg acgatcaagc
     1621 cgggtcccta cccgtggggc aaccacccca acgcatggag gccttcgcac atacatttct
     1681 cgatcatggg accatcgctc ggaatgaggc tggtcaccca gatgtacttt ccgggcgacc
     1741 ccttgattcc gctggacccg atcttcaact ccgtgccggc tcacgccagg tcgcggatga
     1801 tcgccaccta cgatcacgat gtgaccgagg ccaattgggc actcggctat cgatgggaca
     1861 tcgtcctgcg tggagagcag gcgacaccgc acgacgaggg gggcgattga gtcaactgct
     1921 ggggcagacc ccttcgcaga ctgttggccc cttttacacc ctgcggctgt ttcgggaggg
     1981 tgagaacatc ctggtgccac cgggccacac cggaaggatc gtcatccagg gaagggtgct
     2041 cgacggcgat cgatcgccca tagaggacgg gctcctcgag atctggcaag ccaactcagt
     2101 gggccgctac caccatcccg atgacagacg cgacctggca ctggacaacg ggttcaccgg
     2161 tttcggcaga gccataaccg atttcgagac cggggggtac acgttcgaaa cgctcaaacc
     2221 cggaccggtc cccgatgtcg aaggcgcctc tcaggcgcca cacatctcgt tgatcgtgca
     2281 aggacgcgga atgctcaacc cggtgttcac caggatctac ttctcggatg aaaaggaggc
     2341 aaaccgcgac gatctcatcc tgcgctccgt gccggccgag cgccgaggct cgctgatcgc
     2401 agagttggtc gacgatggag agcccagggt gtatcgattc gacgtcctct tccagggtga
     2461 cgacgagacc gtgttcttcg acttctgatg ctcgatccgg gcttcaccac cgaggagatg
     2521 gcgatgatct tcaccccggc ccattgggtc gaggcggttg cacggttcga ggccgccctt
     2581 gccctcgccc tggccgacgc aggcatcgct ccccgtgagg acgccgaggc agtggccgaa
     2641 gcctggcaga ccctggtgat cgaccccgac gacgtccttg ctgccacctg gagcgccgga
     2701 acgccgatga cacccctcct cgaagagatc cgcgggcgtc tgcccgaaga gcgcgcgaag
     2761 tgggcccacc acggctcgac cacccaggac gcggtcgaca ccgccaccat gatcca
//
LOCUS       DASTXO010000486         2835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_802845, whole genome shotgun sequence.
ACCESSION   DASTXO010000486 DASTXO010000000
VERSION     DASTXO010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802845"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="VFU96_10290"
     CDS             <1..368
                     /locus_tag="VFU96_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113695.1"
                     /translation="NSLKRLAPSQQLAPTEAPSSEFIPHELERRVLVPADSAWDESEA
                     FLESVFGALRNPWREPDRPEPAVLQVRVPGESFSLTDDDSPSTSAAEAAVDLRSALTT
                     FDQGRRAAEIATDSVEATA"
     gene            441..749
                     /locus_tag="VFU96_10295"
     CDS             441..749
                     /locus_tag="VFU96_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113696.1"
                     /translation="MCCLATVMVLLGPRLGIIVWGLAQPARWELAFSTFVWPLLGFVF
                     LPWTTLMYVAVAPGGVTGFDWFGIVLAVVADLASYSGGFYGNRDRIQSYTPTAPPMPR
                     "
     gene            complement(799..1947)
                     /locus_tag="VFU96_10300"
     CDS             complement(799..1947)
                     /locus_tag="VFU96_10300"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEU5113697.1"
                     /translation="MSRIAPPRLQTTAEGGHRTATWLELFYDLAFVVAIAILGERLLD
                     DTTWTGFWSFFGYFALLWWLWASHTFYADRYDTDDLVYRLWAAAQMVAVLVMAASLAH
                     GPSESTTVFAAGYAGARLVLLALYWRVYRHVAETRRLVTGYLIGFGAAAVVWVASVFV
                     PDPARFVLWGIALAIDLATPWVVRRQQATVPLDESHLPERFGLFVILVLGESLAAVVA
                     GLSHIGWTTSSLIAAVLGVLLATLLWWLYFDNAEGSVVRRRNIEGKTWRPTGWIYSHL
                     PLAAALAIVGVALDQAILSAGAGPMPPAQRWLLLGGVALALVCLAILQIASLNYSKES
                     VNRVIAINRLAGVPFLALIGLLVQADPVWVLAGATGVCVAVLAADMVA"
     gene            2074..>2835
                     /locus_tag="VFU96_10305"
     CDS             2074..>2835
                     /locus_tag="VFU96_10305"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5113698.1"
                     /translation="MKVSPQPYLPRMVRYWDEDAPGQMQQCLEGSMVFVDISGFTAMS
                     ERLAKHGKLGAEELTAVIASTFGELLGAAYSFGASLLKFGGDALLLFFSGDGYAPRAC
                     AAARAMQQRLDEVGVCYTSAGKITLRMSVGIHTGMFDFFLVGGSHRELIVSGPAATRT
                     VEMEAAAGAGQILLSPGTAEAIPAGNRGRVLGPGVLLKGDVEAERMEIRHATSSTEEF
                     AQFVPIGLCQLLLSGEVEPEHRPAAVAFLHYQGVDD"
BASE COUNT          519 a          910 c          920 g          486 t
ORIGIN      
        1 caaactctct caaaaggttg gcgccgagtc agcagctggc gccgaccgag gcaccatcgt
       61 ccgaattcat cccccatgag ctggaacgtc gggttctggt gccggcagac tcagcctggg
      121 atgagtcgga ggcgttcctc gagagtgtgt tcggagcgtt gaggaacccg tggcgcgagc
      181 ccgatcgtcc cgagcccgcc gtgctccagg tgcgcgtacc gggggagagc ttttcgctga
      241 ccgacgacga ttccccgagc acgtccgcgg ccgaggcagc cgtggacctc aggtcggcgt
      301 tgaccacgtt cgatcagggc cgtcgcgcgg ccgagatcgc cactgattcg gtcgaggcaa
      361 cagcctgaac cgacaacgcc cgcatccacc gtgtaggttg gctgccgccg aagtagtggc
      421 cccgatgaaa ggactctgat atgtgttgct tggcgacggt catggtcctg ctcggcccgc
      481 gtctcgggat aatcgtttgg ggtctcgctc agcccgcccg ctgggaactg gcgttctcca
      541 ccttcgtctg gccgctgctc gggttcgtat tccttccctg gacgacgctg atgtatgtgg
      601 ccgtggcccc cggcggggtg accggcttcg attggtttgg aatcgttctt gcggtcgtgg
      661 ccgatctggc ctcctactcc ggcggtttct acggcaatcg ggaccggatc cagagctata
      721 cgccaaccgc gccacctatg cccaggtagc ccgctcgcca gttcggctgc cccggtcgca
      781 gctccggagc aaattggttc aggcgaccat gtctgccgcc agcaccgcca cgcacactcc
      841 ggtggcgccg gcgagaaccc acaccggatc ggcctggacc agcaaaccga tcaaggcgag
      901 aaacgggact ccggccaggc ggttgatggc gatgactctg ttgactgatt ccttcgagta
      961 gttgagagag gcgatttgca ggatcgccag gcacaccagc gcaagcgcga cgcccccgag
     1021 caacagccaa cgctgagccg gaggcattgg tcccgccccg gcactgagaa tggcctgatc
     1081 gagcgcaacg ccgacgatcg ccaacgcagc cgcgagcgga agatgcgagt agatccaacc
     1141 cgtcggtcgc catgtcttgc cttcgatgtt gcgccgtctg accacgctgc cctcggcatt
     1201 gtcgaagtag agccaccaga gaagtgttgc cagcagcacg cccaagaccg cggcgatcag
     1261 cgaggaggtc gtccacccga tgtggctcaa cccggccacg accgcagcga gtgactcgcc
     1321 cagcaccagg atcacgaaca acccgaagcg ctcggggaga tgcgattcat cgagaggcac
     1381 ggtcgcctgc tggcgtctca cgacccatgg cgtggctaga tcgatggcga gagcaatccc
     1441 ccaaaggaca aatcgagccg gatccggcac gaataccgat gccacccaca ccacggcagc
     1501 agccccgaag ccgatgagat accccgtgac caggcgacgg gtctctgcga cgtgccggta
     1561 cacccgccag tagagggcga ggaggaccag tcgtgcgccg gcgtatccag ccgcgaagac
     1621 ggtcgtcgat tcggacgggc cgtgagccag tgaagccgcc atgaccagga cagcaaccat
     1681 ctgggcagcg gcccacaacc ggtagaccaa gtcgtcggtg tcgtaccgat ctgcgtagaa
     1741 agtgtggctg gcccaaagcc accagagcag ggcgaagtag ccgaagaacg accagaagcc
     1801 agtccaggtg gtgtcgtcga ggaggcgctc tcccaggatg gctatggcca cgacgaaggc
     1861 gaggtcgtag aagagctcga gccatgttgc ggtccgatgc cctccctctg ccgtcgtctg
     1921 cagacgcgga ggagcaatcc tggacatccg ccccgtcctt tgattgttca cgtcggctgc
     1981 tccctcgggc ttgtctcgct gctcgttccg ggacaatatc ccgacgaccc gaagtggaga
     2041 gtggcgcgat caaagcggct ctaacctgaa gtcatgaagg tctcacctca gccctacctg
     2101 ccccggatgg tgcggtactg ggatgaggac gctcccgggc agatgcagca atgcctggaa
     2161 gggtcgatgg tgttcgttga catctccggt ttcaccgcga tgtccgaacg tctggccaag
     2221 catggaaagc tcggggccga ggaactcacg gcggtgatcg cctccacgtt cggcgagctg
     2281 ctcggggccg catattcctt cggggcaagc ctcctcaagt tcggcgggga cgccttgttg
     2341 ctgttctttt ctggcgacgg ctacgcgccc cgagcctgcg ctgcggcccg ggcgatgcag
     2401 cagagattgg atgaggtcgg cgtctgttac accagtgccg gcaagatcac tctgcggatg
     2461 tcggtcggga tccataccgg catgttcgat ttcttcctgg tcggcggctc ccatcgagag
     2521 ctgatcgtct ccggccctgc ggcgactcgc acagtcgaga tggaggcggc agcgggggcg
     2581 ggccagatcc tgctcagccc gggcaccgcc gaggcgatcc ccgcgggcaa ccgagggcgc
     2641 gtcctcggcc cgggcgtcct cctcaagggt gatgtcgaag ccgagaggat ggagatacgg
     2701 cacgcaacat catcgacgga ggagttcgcc cagtttgtgc cgattggcct gtgccagctc
     2761 cttctaagcg gcgaggtgga gcccgagcac cggccggccg cggtcgcctt cctccattat
     2821 cagggcgtgg acgac
//
LOCUS       DASTXO010000487         4490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_103109, whole genome shotgun sequence.
ACCESSION   DASTXO010000487 DASTXO010000000
VERSION     DASTXO010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103109"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /gene="infB"
                     /locus_tag="VFU96_10310"
     CDS             <1..1095
                     /gene="infB"
                     /locus_tag="VFU96_10310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU5113699.1"
                     /translation="GGDIPSVEVSAINGDGVDELLEVVDLIAQLEEYKANPSTEASGV
                     VVESQLERGKGAVASVIIQRGTLRTGDPFVAGAVSGRARALMDENGNQVKEAGPSTPV
                     QIMGWDEVPTAGDFFTVVESDREARRIASEREEQLRSVSQAMPSARDRLQTLLDQLRT
                     EDATLNVIIKADAHGSLEALRDSISKIEREGGKIQVLHGAVGGINENDVTLAEVTDSV
                     IVGFNVRPEPKARKAAEEAGVEIRTYGIIYELLDEVEQMLVGQLAPEEHEQVLGTAEV
                     RAVFKVPRIGSVAGCYVVEGVVQRGARARLIRDGVVIHDGVISSLKRFKDDVREVAAG
                     FECGIGLENYNDVKEGDNIEAYLIREVART"
     gene            1095..1388
                     /locus_tag="VFU96_10315"
     CDS             1095..1388
                     /locus_tag="VFU96_10315"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEU5113700.1"
                     /translation="MRAAALRVELHLPAPQSLKEKRAVLRPVIEGLRRLGSYSVAEVD
                     HHDKWQRAAIGVAIAAPDGQSLAMQVSKLHRYLDGQLEVEVLDVRMSELEEPE"
     gene            1385..1729
                     /gene="rbfA"
                     /locus_tag="VFU96_10320"
     CDS             1385..1729
                     /gene="rbfA"
                     /locus_tag="VFU96_10320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU5113701.1"
                     /translation="MRDASPRMLKVNSTLREVLAEEIERMSDSRLEMVSITGVETSPN
                     LRHAVVYIDVLGADATDALTALDHASHRLQSVIARQVRMKYTPVLEFEVDPGVSGGER
                     IDAILRSLHEEE"
     gene            1737..2708
                     /locus_tag="VFU96_10325"
     CDS             1737..2708
                     /locus_tag="VFU96_10325"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEU5113702.1"
                     /translation="MIERQLIEKAAATIAASRELVLACHVGPDGDALGSMLGFGIAAR
                     AAGKTVVASFGSPFVVPANLSFLPIDLLVDPSEFPKSPETMVVFDAGSADRLAELGAP
                     AGNAGTLVVVDHHVTNEGFGDIPLVDPGAAATGVIVADLLDALGWPITPEIATCLLTA
                     VVTDTGRFQYANTTAPTLSLAARLVDAGARPEQISQKVYEEAPFGYLKAAGAALSRAI
                     LDSELSMVSTIITHQDLQDASVDWGDIDNLINTLRLAEEADVAVLAKVNGDGHVKVSL
                     RSRGATDVGALAAELGGGGHRLASGFTFEGDPEMAIDEVRNRLEAHR"
     gene            2705..3583
                     /gene="truB"
                     /locus_tag="VFU96_10330"
     CDS             2705..3583
                     /gene="truB"
                     /locus_tag="VFU96_10330"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU5113703.1"
                     /translation="MTRGFLVVDKPRGMTSNAVVGRVKRATGIKKVGHAGTLDPLATG
                     VVVVAVGPVTRLIRFIQEQPKEYLATAQFGVSTDTLDSDGAVLTREPMEFSEEELEAV
                     AGRFVGTVLQVPPMVSALKHQGRRLYELARQGEVVEREARPVEILELEIESVGAGPYP
                     EVEFRVVCGKGTYVRSLADDLAAALGGSAHLTSLRRTRTGSLDVDKHGVQIDELGQWE
                     SYFLTPSQALGDLPAVTVSDETARGVCHGMQFVGGEMIAAPDHSVIRVLDEAGDLIAV
                     YRRDGEQARPEVVLAS"
     gene            3580..>4490
                     /locus_tag="VFU96_10335"
     CDS             3580..>4490
                     /locus_tag="VFU96_10335"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU5113704.1"
                     /translation="MKVLRGPLERWEAQTPPNAVTVGVFDGVHLGHRVILDRLLENSH
                     PSTVLTFDPHPAEVLSPGTNPRLITTIDERIALLSDLGIDTIGVLDLAEIRHLEPEEF
                     VSQVLVDQLEVSYLVVGADFQFGRDRAGDCDFLIGAGTRHGFDVEVVALVESSGVISS
                     TRIRRLIEDGDVSAAADLLGRLYRLSNVVAPGDSRGKDLGFPTANLKPPERKVVPGNG
                     IYAAWARIGRETLPAAVNVGVRPTFGGTELLIEAHIIDFEREIYGSVLTLDFVEKLRP
                     ELQFENADALVAAIRHDVAQVRRLLKTA"
BASE COUNT          812 a         1427 c         1466 g          785 t
ORIGIN      
        1 ggcggtgaca tccccagtgt ggaagtttcc gccatcaacg gtgacggcgt cgatgagctg
       61 ctcgaggtgg tggacctcat cgcccagctc gaggagtaca aggccaaccc gagcacagag
      121 gcgtccgggg tcgtggtcga gtctcagctg gagcggggca agggtgcggt cgcctcagtc
      181 atcatccagc gcgggacctt gaggaccggc gaccccttcg tcgcgggggc ggtctccggg
      241 cgtgcccgcg ccctaatgga cgagaacggg aaccaggtca aggaagcagg cccgtcgaca
      301 ccagttcaga tcatgggctg ggacgaggtc ccgaccgccg gcgatttctt caccgtggtc
      361 gagagcgatc gggaggcgcg tcgtatcgcc tccgagcgtg aagagcagct cagatcagtc
      421 agccaggcca tgccctcggc ccgcgatcgt ctccagacac tcctcgacca gctgcggacc
      481 gaggacgcca ccctgaacgt catcatcaag gccgacgccc atgggtcctt ggaagcactg
      541 cgcgattcga tctccaagat cgaacgagaa ggcggaaaga tccaggtcct gcatggagca
      601 gttggtggga tcaacgagaa cgacgtgacc ctcgccgaag tgaccgattc agtgatcgtc
      661 ggcttcaacg tccgtcccga gcccaaggcc cgcaaggccg ccgaggaggc cggggtcgag
      721 atccggacgt acgggatcat ctatgagctc ctcgatgaag tcgagcagat gctggtcggc
      781 cagctcgctc ctgaggaaca tgagcaggtc ttgggcaccg ccgaagtcag ggccgtgttc
      841 aaggtcccgc ggatcggctc ggtcgccggt tgctacgtgg tcgaaggcgt cgtccagcgc
      901 ggggcccgcg cccggctcat ccgtgacggg gtggtcatcc acgacggcgt catctcgtcg
      961 ctgaagcgat tcaaggacga cgtccgtgag gttgccgccg ggttcgagtg tgggatcggg
     1021 ctggagaact acaacgacgt caaggaaggc gacaacattg aggcctacct gatccgtgag
     1081 gtagcccgga cctgatgcga gctgcggcct tgagggtcga gctgcacctg cccgccccac
     1141 agtcgctgaa ggagaagcga gccgtgctca gaccggtcat cgagggcctt cgacgactcg
     1201 gctcgtacag cgtcgccgag gtcgatcacc acgacaagtg gcaaagggcg gcaatcggag
     1261 tcgccatcgc cgcccctgat ggtcagagcc tcgccatgca ggtgtcgaag ttgcaccgct
     1321 accttgatgg gcaactggag gtcgaagtcc tcgacgtgcg catgagcgag ttggaggagc
     1381 ccgaatgaga gatgccagcc cgcgaatgct caaggtcaac tccaccctcc gggaagtcct
     1441 cgcagaagag atcgagcgaa tgagcgattc aaggctcgag atggtgtcca tcaccggagt
     1501 cgagacaagt cccaatctcc gtcacgccgt ggtctacatc gacgtcctcg gcgccgatgc
     1561 caccgacgcg ctcaccgccc tcgatcacgc ctcacatcgt ctccaatccg tcatcgcccg
     1621 tcaggttcgg atgaagtaca cgccggtcct ggagttcgag gtggatcccg gcgtgagcgg
     1681 aggcgagaga atcgatgcca tcctgcgctc cttgcatgag gaggagtgag agccggatga
     1741 tcgaacgaca gctcatcgag aaggcagcag ccacgatcgc ggccagtcgg gaattggtgt
     1801 tggcctgtca tgtcgggcca gatggagacg ccctgggctc gatgctcgga ttcggcatcg
     1861 cggcgcgcgc tgccggcaag accgtggtcg cctccttcgg gtctccattc gtcgtgccgg
     1921 caaacctctc attcctcccc atcgacctgc ttgtcgaccc gagcgaattc cccaagtctc
     1981 ccgagaccat ggtcgttttc gatgccgggt cagccgaccg tcttgccgag ctcggcgcac
     2041 cggcaggtaa tgccggcacc ctcgtggtgg tcgaccacca cgtcaccaac gagggatttg
     2101 gtgatatccc tctcgtcgat cccggcgccg cggcgaccgg agtgatcgtg gcagatctgc
     2161 tcgacgccct gggatggccg atcacacccg agatcgccac ctgtctcctc accgcggtgg
     2221 tcaccgacac cggccgcttc cagtatgcga acaccactgc tccgactctg agtctggctg
     2281 cccgtctcgt cgacgccggt gcccggcccg agcagatcag tcagaaggtg tacgaggagg
     2341 cgcccttcgg gtacctgaag gctgccggag ccgcgttgag ccgagccatc ctcgactccg
     2401 agctcagcat ggtgtccacg atcatcaccc atcaggatct ccaagatgcg agcgtggact
     2461 ggggcgatat cgacaacctg atcaacacgc tcaggctcgc cgaggaggcg gatgttgccg
     2521 tcctggcgaa ggtcaacgga gacggtcacg tgaaggtcag cctccgctcc cgcggtgcca
     2581 ccgatgtggg agccctcgca gccgagctcg gtggtggcgg gcatcgactc gcctccgggt
     2641 tcaccttcga gggcgacccg gagatggcaa tcgacgaggt gcgaaacagg ctcgaggctc
     2701 accgatgacc cggggattcc tggtcgtcga caagccgagg ggtatgacat ccaatgcggt
     2761 cgttggccgg gtcaagcgcg caaccggcat caagaaggtg ggtcacgccg gcactctcga
     2821 ccctcttgcc accggcgtcg tggtcgtcgc agttggcccg gtgaccagac tcatccggtt
     2881 catacaggaa cagcccaagg agtatctagc gaccgcccag ttcggagtgt ccaccgacac
     2941 cctcgactcc gacggagcgg tattgacccg cgagcccatg gagttctccg aggaggagct
     3001 ggaggcggtg gctggccgtt tcgtcggcac ggtgctccag gtgcctccga tggtctcggc
     3061 cctgaagcac cagggccggc gtctctacga gttggcccgc cagggcgaag tggtggagcg
     3121 tgaggcccgg ccggtggaga tcctcgagct cgagatcgag tccgtgggtg ccggccctta
     3181 ccccgaggtc gaattccggg tcgtgtgcgg aaaggggacc tatgtgcggt cgctggccga
     3241 tgacctggcc gcggctctgg gcgggtcggc ccatctcacg tcactccgcc gtactcgcac
     3301 cggctccctc gacgtggata aacatggggt ccagatcgac gaactcggtc aatgggagtc
     3361 ctatttcctc acgccatcgc aagccctcgg cgacctaccg gccgtaaccg tgagtgacga
     3421 gacggcgcgg ggggtttgcc acggcatgca attcgtcggc ggcgagatga ttgccgcccc
     3481 ggatcattcg gtgatcaggg tcctcgatga ggcaggcgat ctcatcgccg tgtaccggcg
     3541 tgacggggaa caggcgagac ccgaggtggt gctcgcctcg tgaaggtcct tcgaggcccg
     3601 ttggagcgct gggaggcgca gacaccgccg aacgcggtca cggttggcgt gttcgatggc
     3661 gttcacctcg ggcaccgtgt catcctcgac cggttgctgg agaactccca tccgtcgacg
     3721 gtcctcacct tcgaccctca tccggccgaa gtgctctcac cgggcaccaa tccccgtctg
     3781 ataacaacca tcgatgagcg aattgccctg ctctccgatc tcggcatcga cacgattgga
     3841 gtgctcgacc tcgccgagat ccgccacctc gagcctgagg agttcgtctc tcaggtgctc
     3901 gtggaccagc tcgaggtcag ctacctggtg gttggggcgg acttccagtt tggccgcgat
     3961 cgagccggtg actgcgattt cctcatcggg gcagggacac gtcacggttt cgatgtcgag
     4021 gtggtcgccc tcgtcgaatc gagcggcgtc atctcatcga cccgaatccg gagactgatc
     4081 gaggatggcg atgtctcggc tgcggcggac ctcctcggca ggctctaccg cctttcaaat
     4141 gtcgttgctc ctggtgacag caggggcaag gatctcgggt tcccaacggc caacctgaaa
     4201 cccccggaac gcaaggtggt ccccgggaat ggcatctacg cagcctgggc ccgaatcggg
     4261 cgtgagaccc tgccggcggc ggtgaacgtc ggggtcagac ccaccttcgg cggcaccgag
     4321 ctgctcatcg aggcgcacat catcgacttc gagagggaga tctacgggtc cgtcctcact
     4381 cttgacttcg tggagaagct ccgcccggag ctgcagttcg agaacgccga tgccctcgtc
     4441 gccgccatca ggcacgatgt ggctcaggtg cgccgtctgc tcaagacggc
//
LOCUS       DASTXO010000488         5874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1281659, whole genome shotgun sequence.
ACCESSION   DASTXO010000488 DASTXO010000000
VERSION     DASTXO010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281659"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VFU96_10340"
     CDS             <1..467
                     /locus_tag="VFU96_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006142152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5113705.1"
                     /translation="VSTAGSGESGIALLIDQAYHLFLPALTLTLAYLGEYTLVTRSSL
                     LDVMGEDYLTVARAKGLRDVAVRNRHAVPNALLPVVTLTAINFGFVLSGAIAVEAVFS
                     WPGLGKLTADAIRGPDLPMLQALFLLFSAAVIVFNLIADLTYSWLDPRVEGQ"
     gene            464..1357
                     /locus_tag="VFU96_10345"
     CDS             464..1357
                     /locus_tag="VFU96_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010310638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5113706.1"
                     /translation="MTSLAMQRRRATAGRVWKQFRGSRGGMIGLVVLLLFVLMALAAP
                     LLADVRELNPVYARAQGNPQWAPPSEFGPMGTDYIRRSVWAQFVWGSRVSLFVGLAAT
                     VLTILIGSVIGIISGFVGGKPDAVLMRITEWFLVVPFLPLAIVLASVLGRNVWNIIFV
                     IGVTSWPSTARLIRAQVLTVKQRLFVDRARSLGASGPHIVGQHILPNVMGLILANATL
                     AVPISILTETTLAFLGLGDPSAASWGKTLEEAFVNGAMVRNAWWYFVPAGLGILAVVL
                     AFTLIGRTLEEIANPRLSRSR"
     gene            1354..2316
                     /locus_tag="VFU96_10350"
     CDS             1354..2316
                     /locus_tag="VFU96_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019764433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5113707.1"
                     /translation="MSDLMLSVRDLEVTYHGHGGDVPAVRGVSFDLEKGDSLGLAGES
                     GCGKSTLANALLRLLPAGTEVTGQITLDGKDVLKLTPGGLRAARWTSGAIIFQGALHA
                     LNPVQTVGEQIAEPMVIHQTAASDNAARERAVELLRRVGLPAARAESYPHQLSGGQRQ
                     RVLIAMALACDPLLLIADEPTTALDLMVQAQVLALLDDLKKERDLTLMFITHDLSVIT
                     YACRRLAVMYAGRIVEEGPAWDVFENASHPYTRALAAAFPTIGDETSRMAPSGLGGDP
                     PTPDNLPRGCPFHPRCPLAHDACLPVEPELRSSGAERSAACVLV"
     gene            2301..3263
                     /locus_tag="VFU96_10355"
     CDS             2301..3263
                     /locus_tag="VFU96_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017590997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5113708.1"
                     /translation="MRAGVTNLVEVEELHVHFGAARAVDGASLHVSPGEVLALVGESG
                     SGKTTLIRAIQGLQKPTSGQIRVEGEGDGAPRPKVQTIFNPSVQMVFQDPTGALNPRQ
                     TIYEIVAEGLRIKHDADSEQDRVAQALSRAGLRPPERFFPLYPHEVSGGQRQRVVIAG
                     AMVMEPRLLLADEPVASLDASIRGEILGLMRRLVDETGLAILVVTHDLGLAWNIADRV
                     AVMYLGRIVETGPTEQILENPRHPYTRALLSVVPETREMEQQILTGEIPDPARIPSGC
                     RFHPRCPLLQSGTLAPEIAARCPTDDPGPTAVGALTAAACWAVP"
     gene            complement(3273..5057)
                     /locus_tag="VFU96_10360"
     CDS             complement(3273..5057)
                     /locus_tag="VFU96_10360"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing sensor
                     histidine kinase"
                     /protein_id="HEU5113709.1"
                     /translation="MKRRRVQEALEDTGRLAALNELSAALYEYDSAEAVTVADSAIAL
                     ADAIGDGAARAWALHHRGWALSSMGRLDDALDSQLSARAQFELEGDMGGVANALMAIG
                     DLYGDVGDTATALEYLDRARAPLKLADDELGVGLLTNLTGIALSHEGRHEEALDLFEQ
                     AEAAYLRLGDGLRVGTAKINQGFELLALVEESEEGDQVIDRVAVLAEEIIERGEQLGD
                     DGRHTVAYGRALQAHVLAATRPIDDAIDAAVIAEMEAASGGLDQLAVELALDRAGWLI
                     GVGRNEEAAMLVEEAADRSKRLDMRRLLVRATSLRADLLEAMGDYTGALACHREYHRL
                     DGDLHTDQAERRASLATTRFQVERARQEAELARIRVSELEALDHDKREFLASISHELR
                     TPLAAVLGFATELSDSWEVFEPEEARSLVRLIASQSADISSIVDDLLTVTRLEAGTMT
                     FYPSAVSVAEHLADMVETSGRESGRSISWKGDAMVWADPNRLRQIVRNLITNAIRYGG
                     EEISVCVMSHPTGASIDIKDSGGPIPAGRAATMFDPFVHADSGGRTPNSVGLGLAVAR
                     SLARIMGGDLDYRHDGGSVFRLALPVPE"
     gene            complement(5124..>5874)
                     /locus_tag="VFU96_10365"
     CDS             complement(5124..>5874)
                     /locus_tag="VFU96_10365"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5113710.1"
                     /translation="LYQERSGIAMASRANSAHRRLAVDNGATLVDGARVGSIRELDGE
                     VEIEVDGTVHRVGSLIVAAGAWTTGVLHQIGVDLPLEVTLEQIVYMEAGEPALFHPSR
                     FPIWIWMDEPSFYGFPIFGEPAVKIAWDRCEIVTDPETRSFEPRDDVTEAVRGFAAEH
                     LPGAVGPVRLAKTCLYTLTPDRDFVVDRVPGTDNIFSVVGAGHAFKFASLLGRILVDL
                     ALDGSTTHDITALAADRPILREKEPERSYMV"
BASE COUNT          933 a         1968 c         1888 g         1085 t
ORIGIN      
        1 gggtgtccac tgcgggatcc ggcgaatccg gcatcgcgct gctcatcgat caggcctatc
       61 acctgtttct tccggcgctg acgctgacac tggcctacct cggggagtac acgctggtca
      121 cccggagctc tttgctcgat gtgatggggg aggactatct gacagtggcc cgggccaagg
      181 gattgcgtga cgtggcggtc cgcaaccgcc atgccgtccc caacgcgttg ctccccgtgg
      241 tgaccctcac cgccatcaac ttcgggttcg tcctctcggg cgccatcgcg gtcgaggcgg
      301 tcttttcctg gcccgggttg ggcaagctca ccgccgacgc catccgcggc cctgacctcc
      361 ccatgcttca ggcgctcttt cttctcttca gcgcggcggt gatcgtcttc aatctgatcg
      421 ccgatctcac ctacagctgg ctcgacccac gggtggaggg ccaatgacga gtctcgccat
      481 gcagcgccga cgcgccaccg ccggtcgggt gtggaagcaa ttccgtggtt ccaggggggg
      541 catgattggc ctcgtcgtcc tgctgctgtt cgtcttgatg gccttggcgg cgcctctgct
      601 cgccgatgtc agggagttga acccggtcta tgcccgggcc caagggaatc cgcaatgggc
      661 acccccgagc gaattcggtc ctatgggcac cgactacatc cgccgctcgg tgtgggcgca
      721 gttcgtctgg ggctcccggg tcagcctctt cgtcgggctg gcggcgacgg tcctcacgat
      781 cctgatcggc tcggtcatcg gcatcatctc cggattcgtc ggtggcaagc cggacgcggt
      841 gctgatgcgg atcaccgagt ggttcctggt tgtccccttc ctccccctgg ccatcgtgct
      901 ggcctccgtg ttggggagaa acgtatggaa catcatcttc gtcatcggtg tcacttcctg
      961 gccgtcgact gcgcgtctca tccgcgctca ggtgctcact gtcaaacagc gtctcttcgt
     1021 cgaccgggcg cgctcgctcg gcgcgagcgg cccgcacatc gttgggcagc acatcctgcc
     1081 caatgtgatg ggactgatcc tggccaacgc cactctcgcc gtcccgatct ccatcctcac
     1141 cgagaccacg ctggcgttcc tcgggctggg ggacccgtcg gccgcatcat ggggcaagac
     1201 tctggaggag gcgttcgtca acggagccat ggtgcggaac gcctggtggt atttcgtccc
     1261 cgctggtctg ggaatcctgg cggtggtcct cgccttcacc ctcatcgggc gcactctcga
     1321 ggagatagcc aatccaagac tgagccggtc tagatgagcg atcttatgct ctcagtgcgt
     1381 gacctggagg tcacctatca cgggcacgga ggcgacgtgc cggcggtgcg cggggtcagc
     1441 ttcgacctgg agaagggaga ttcgctgggt ctcgccggcg agtcgggctg cggcaagtcc
     1501 acgctggcca atgcgttgct ccgcctcctc cccgccggca ccgaggtgac cgggcagatc
     1561 accctggatg ggaaggatgt cctcaaactc actcccggag ggctgagggc cgcccggtgg
     1621 acgtcgggag ccatcatctt ccagggggcc ctccacgcgc tcaacccggt ccagaccgtg
     1681 ggcgagcaga tcgccgagcc gatggtgatc caccagacgg cggccagtga caatgcggcc
     1741 cgcgaacgtg ctgtcgagct gctgaggagg gtcggcttac ctgcagcccg ggccgagtca
     1801 tacccacacc agttgtcggg aggacagcgg cagcgggtcc tcatcgccat ggcgctggcc
     1861 tgtgacccac ttctcctaat cgccgacgag cccacaactg cgctcgacct catggtccag
     1921 gctcaggtgc ttgctctcct cgacgatctc aagaaggagc gggatctgac attgatgttc
     1981 atcacccatg atctctcggt gatcacctat gcctgccggc gcctggcggt gatgtatgcg
     2041 ggacggatcg tggaggaggg cccggcttgg gacgtgttcg agaatgcctc ccatccctat
     2101 accagggcac tcgccgcggc tttcccgacg atcggggacg agacttcgcg gatggcgccg
     2161 tccggcctgg gtggtgaccc gccgacgccc gacaacctcc ctcgaggatg tcccttccac
     2221 ccccgctgtc ctctggctca tgatgcctgt ttgccggtcg agcccgagct gcgttcgtca
     2281 ggcgcggaga ggagcgcggc gtgcgtgctg gtgtgaccaa cctggtcgag gtcgaggagc
     2341 tgcacgtcca tttcggggcg gcccgggccg tggacggggc atcgcttcac gtctcgcccg
     2401 gcgaggtgct cgccctcgtc ggcgagtcgg gatcggggaa gacgaccctc atccgggcga
     2461 tccagggcct gcagaagccg acttccggtc agattcgcgt cgaaggtgaa ggcgacggtg
     2521 cccccagacc caaggtccag acaatcttca atcccagtgt gcagatggtc ttccaggacc
     2581 ccacgggtgc cctcaatccc cgccagacca tctacgagat cgtcgccgag gggcttcgca
     2641 tcaagcacga cgccgacagc gagcaggatc gagtggcgca agcgctctcc cgggccggtc
     2701 ttcgcccacc ggagcgattc ttccccctct atccacacga ggtgtccggg gggcagcgtc
     2761 agcgtgtggt gatagccggg gcaatggtca tggagccacg cctcttgctg gcagacgaac
     2821 ctgtggcgag tctcgacgcc tccatccggg gtgagatctt gggcttgatg cgccgcttgg
     2881 tggatgagac tggcttggcc atcctcgttg tcacccacga tctcggcttg gcatggaaca
     2941 tcgccgaccg cgtcgctgtc atgtatttgg gccgcatcgt cgagaccggg cccaccgagc
     3001 agatcctgga gaacccgaga cacccgtata cccgtgcgtt gctctcggtg gtgcccgaga
     3061 cgcgcgagat ggaacagcag atcctgacgg gggagatccc cgatccggcg cggatcccat
     3121 ctggatgccg attccatccc cggtgccccc ttctccagtc cgggactctc gcgcctgaga
     3181 tcgccgctcg atgccccacg gatgacccgg gtccgaccgc cgtaggcgct ctcaccgcag
     3241 ccgcttgctg ggcagtcccc tagccgggca ggtcactcag ggacagggag ggcgaggcgg
     3301 aacaccgatc caccgtcgtg tcggtaatcg aggtcgccac ccatgatccg ggccaggctc
     3361 ctcgccacgg cgaggccgag ccccacagag ttcggggtac ggcccccgct gtcggcgtgg
     3421 acgaaggggt cgaacatggt cgccgcccga cctgccggga tcgggccccc cgaatccttg
     3481 atgtcgatgc tcgctcctgt ggggtgtgac atgacgcaga ccgagatctc ttcgcctccg
     3541 taacggatgg cgttggtgat gaggttgcgc acgatttgcc gcagtcggtt cggatccgcc
     3601 cacaccatgg cgtctccctt ccacgagatg cttcgaccgg actcccgtcc cgacgtctcc
     3661 accatgtcgg cgaggtgctc ggcgacgctc acggccgacg ggtagaacgt catggttccg
     3721 gcttcgagcc tggtgacggt caacaagtcg tccacgatgc tggagatgtc ggccgattgg
     3781 ctggcgatga ggcggaccag ggagcgcgcc tcctcgggct cgaagacctc ccaagagtcg
     3841 gagagctctg ttgcgaagcc gaggaccgcg gcgagcggtg tgcgcagctc atggctgatg
     3901 ctggcgagga actcccgctt gtcgtggtcc aacgcctcca gctcggaaac ccgaatcctg
     3961 gccagctcag cctcctgacg agcacgttcg acctggaacc gcgtggtagc gagactggct
     4021 cgccgctccg cctgatccgt gtgaaggtct ccgtcgagac ggtggtattc ccgatggcag
     4081 gccagcgccc cggtgtagtc ccccatcgcc tcgagcaggt cggctcgcag ggaggtcgcc
     4141 ctcaccagca gccgcctcat gtccaaacgc ttgcttcgat cggcggcttc ctccaccagc
     4201 atcgccgcct cttcgttgcg gccgacgccg atgagccagc cggcgcgatc gagagcgagc
     4261 tcgaccgcca gctggtcgag ccctccggag gcggcttcca tctcggcgat cacggctgca
     4321 tcgatcgcat cgtcgatcgg gcgggtggcg gcaaggacat gggcctgcaa tgccctcccg
     4381 taggcaacgg tatggcgccc gtcgtccccg agttgctcgc ctctctcgat gatctcctcg
     4441 gcaagaaccg cgacccgatc gatgacctgg tccccttctt cgctctcctc gacgagggcg
     4501 aggagctcga acccctggtt gatcttcgcc gtgccaaccc gcagcccatc gccgagcctc
     4561 agataggcgg cctcggcttg ctcgaacaga tccagggctt cctcgtgtct cccctcgtgg
     4621 ctcaatgcga tcccggtgag gttcgtcagc agcccgacgc cgagctcgtc gtctgccagc
     4681 ttcagaggtg cccgagcccg atccaggtac tccagcgccg tcgcggtgtc gccaacatcc
     4741 ccgtacaagt cgccgatcgc catcagggca ttggccactc cacccatgtc cccttccagc
     4801 tcgaactggg cgcgagcaga caattgcgag tcgagggcgt cgtcgagacg ccccatggag
     4861 ctcaaggccc agccccggtg gtgaagggcc catgcccgtg ccgctccgtc accgatcgcg
     4921 tcggccagcg cgatggcaga gtcggccacg gtcaccgcct cggcagagtc gtactcgtac
     4981 aaggcggccg acagctcgtt gagggcggcg agccgtcccg tgtcttcgag cgcttcctgg
     5041 acgcgccttc tcttcatgga gagagagtcc atctcgggaa ggaatcggcc cttttacggg
     5101 ggtacttgac cgtggcgagg tcgctagacc atgtaggagc gttcgggttc tttctcccgg
     5161 agaatgggcc ggtcggcggc cagagccgtg atgtcgtgcg tcgtggagcc gtcgagagcg
     5221 agatcgacga ggatccgacc gagcagcgaa gcgaacttga aggcatggcc cgcaccgacc
     5281 acggagaaga tgttgtcggt gcccggtacc cggtcgacga cgaaatcgcg gtccggggtg
     5341 agcgtgtaga gacaggtctt tgccagccga accggtccta ccgctccggg cagatgctcg
     5401 gccgcgaacc ctctcaccgc ctcggtcaca tcgtcgcgtg gctcgaagct gcgggtctcg
     5461 gggtcggtca cgatctcaca tcgatcccag gcgatcttga ctgccggttc cccgaatatc
     5521 gggaagccgt agaacgaggg ctcgtccatc cagatccaaa tggggaatcg ggagggatgg
     5581 aacaacgcag gctctccagc ctccatgtac acgatttgtt cgagggtcac ctcgaggggg
     5641 agatccactc cgatctgatg taggacccct gttgtccatg ccccggcggc gacgatcaac
     5701 gagcccaccc ggtggaccgt gccgtcgact tcaatctcga cttcgccgtc gagctctcgg
     5761 atggagccga cccgggcgcc atcgaccagg gtcgcaccgt tgtccacggc gaggcggcga
     5821 tgggcggaat tggccctcga agccatggcg atgcccgatc gctcctgata gagc
//
LOCUS       DASTXO010000489         2988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_619023, whole genome shotgun sequence.
ACCESSION   DASTXO010000489 DASTXO010000000
VERSION     DASTXO010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619023"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VFU96_10370"
     CDS             complement(<1..513)
                     /locus_tag="VFU96_10370"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HEU5113711.1"
                     /translation="MPEDQSPAEVEETPSSPEIEEKSVSKQYSGKFGRRKVSYTATAA
                     TQIVEQDDDKKAVFFYVAYTEDGADPTTRPILFAFNGGPGSSTVWLHLGLLGPRRVEF
                     DENGFKISAPGRLIHNEQSILDVADMVLIDAIGTGFSTATPKDKEKEFHHFSKDIGAF
                     SDFIVSYLNRN"
     gene            complement(523..795)
                     /locus_tag="VFU96_10375"
     CDS             complement(523..795)
                     /locus_tag="VFU96_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase"
                     /protein_id="HEU5113712.1"
                     /translation="MVDRFAEDWKSAGLDDPTKSLLLFAERLTDECAAVGPEDIAVLR
                     GHGFDDKAISSCVQVVAYFNYINRIAEGLGVAAEGWIDDNGRVKTT"
     gene            complement(844..1074)
                     /locus_tag="VFU96_10380"
     CDS             complement(844..1074)
                     /locus_tag="VFU96_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU5113713.1"
                     /translation="MTAADVATGVTKIEYEAATRRAGRIWNIVSIQSQLPEVMRDSMR
                     LYSTIMFGDSPLSRAQREMIAVVTSQVNECHY"
     gene            complement(1095..2351)
                     /locus_tag="VFU96_10385"
     CDS             complement(1095..2351)
                     /locus_tag="VFU96_10385"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEU5113714.1"
                     /translation="MAREFLLPDIGEGLTEAEIVRWLVPEGERVEADQPVVEVETDKA
                     VVEIPSPYAGTVLRHGGAEGETIRVGSVLVVIGEEGEAEVPTPDVPEPEPSHAAAPIV
                     GSLDEEAEVLSPVGGTNRDEATAAVKALPIVRKLAREMGVDLGQVQGSGPEGRITRED
                     VLAAGPPTETETPSREPSGHAVATASPSPVAGQDERRPLSRLRRTIAANMAKSWAEIP
                     HVTTFDEVDATRLIEIKSALGARHDVKLPMEALVIKAVIPALEAFPEFNATLDGDDLI
                     VHVAHHVGIAVDTPDGLLVAVIRDAGSRPVLDIASEVRRLGAGARERSLAPDELSGQT
                     FTVSKIGAVGGGQGTPIVPYGTTAILSVGRAQEKVVVANGDLAIAPVMPLSLSYDHRV
                     IDGAQGRRFMALVIENLEEPALFLAG"
     gene            complement(2351..>2988)
                     /locus_tag="VFU96_10390"
     CDS             complement(2351..>2988)
                     /locus_tag="VFU96_10390"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEU5113715.1"
                     /translation="IRVPFGGGIGAAEHHSESTEAIYAHIPGLKVIVPSTPSDARGLL
                     LAAIEDPDPVLFLEPIRLYRAIKEEVPESYYTTEIGPLRVEREGEDITLVAWGAMMKE
                     TRAAATRLEESGASVEVLDLRTLSPIDAGGIVRSATKTGRVVVIHEAPRNAGLGAEVA
                     AIVQEQALYSLAAPVRRVTGWDTIFPLKRSEGHYLPTVDRIVRVAQETLEA"
BASE COUNT          484 a          993 c          953 g          558 t
ORIGIN      
        1 attccggttc agataggaca cgatgaagtc gctgaaggcg ccgatgtcct tggagaagtg
       61 gtggaactcc ttctccttgt ccttgggcgt ggccgtggag aagcccgtac cgatcgcgtc
      121 gatcaagacc atgtcggcga cgtcgagaat cgactgctcg ttgtggatca gccggcccgg
      181 cgcggagatc ttgaagccgt tctcgtcgaa ctccacccga cgcggcccga gcagcccgag
      241 atggagccag acggtggacg agcccggtcc cccgttgaag gcgaacaaga tcggccgggt
      301 tgtcggatcg gctccgtcct cggtgtaggc cacgtagaag aagacggcct tcttgtcgtc
      361 gtcctgctcg acgatctggg tggcggcggt ggcggtgtat gacaccttgc ggcggccgaa
      421 cttcccgctg tactgcttgg agacgctctt ctcttcgatc tccggagagg agggcgtctc
      481 ctcgacctcg gcggggctct gatcctcagg catgacgcgt atctaagtcg tcttcacccg
      541 cccgttatcg tcgatccatc cctcggctgc caccccgagg ccctcggcga tgcgattgat
      601 gtagttgaag taggcgacaa cctggacaca gctcgatatg gctttgtcgt cgaacccgtg
      661 gcccctgagc acggcgatgt cctctgggcc gactgcggcg cactcgtcgg tcagacgctc
      721 ggcgaagagg agaagggatt tcgtggggtc gtcgagccct gcgctcttcc aatcctcggc
      781 gaacctgtcc accatctgtt gatcgtcgac ctcatcccgg aggtcgtgca agtgcgccgt
      841 tattcagtaa tggcactcgt tcacttgcga ggtgaccacg gcgatcattt cgcgttgcgc
      901 ccggctcagg ggcgaatcgc cgaacatgat cgtggagtag agccgcatcg aatcccgcat
      961 cacctccgga agctggctct ggatcgacac gatgttccag atccgccctg cccgcctcgt
     1021 cgcggcttcg tactcgatct tggtgacacc tgtcgccacg tctgcagccg tcacgtgaat
     1081 gtgcggcaag gctctcagcc ggcgaggaac agcgccggct cctccagatt ctcgatgacc
     1141 agcgccatga atcgtcgccc ctgggctcca tcgatcaccc ggtggtcgta ggagagcgag
     1201 aggggcatca cgggggctat ggccagatct ccgttggcaa ccacgacctt ctcctgcgcc
     1261 cttcccaccg agagaatcgc cgtcgtcccg taggggacga tcggcgtccc ctggccgcca
     1321 ccgacagccc cgattttgga gaccgtgaac gtctggccgg acaactcgtc gggggccagt
     1381 gaacgctctc gtgctccggc cccgaggcgg cgcacttcgg aggcgatatc gagcacgggg
     1441 cgcgacccgg catcgcgtat caccgcgacg agaaggccat ctggcgtgtc gacggcgatc
     1501 ccgacgtgat gggcgacatg gacgatcagg tcgtcgccat cgagggtggc gttgaactcg
     1561 ggaaaagcct cgagggcggg gatcactgcc ttgatcacca acgcctccat ggggagcttc
     1621 acatcgtgac gagcgccgag agcggacttg atctctatga gccgggtggc gtcaacctcg
     1681 tcgaaggtgg tgacatgggg tatctcggcc cagctcttgg ccatgttggc tgcgatcgtc
     1741 cggcgcaagc gtgacagggg ccgacgctcg tcctgaccgg caacgggcga gggcgatgcc
     1801 gtcgccacag cgtgccccga cggttcgcgc gacggagtct ccgtttcggt gggtgggccg
     1861 gccgccaaca cgtcttcccg ggtgatcctg ccctcagggc ctgatccctg gacctgcccg
     1921 agatcgacac ccatctcccg ggccagcttc cgcacgattg gaagggcttt gaccgccgcc
     1981 gttgcctcgt cacgattggt ccctccaacc ggggagagga cttcggcctc ttcgtcgaga
     2041 ctgccgacga tcggcgctgc cgcgtgagac ggctcaggct cgggcacatc gggtgtcggg
     2101 acctcggcct cgccctcctc tccgatgacg accaggacgg atcccactcg aatcgtctct
     2161 ccttctgccc cgccatgtcg caggaccgtg ccggcatacg gggaagggat ctccaccacg
     2221 gccttgtcgg tctcgacctc gaccaccggt tggtcggcct cgacccgctc accttcgggg
     2281 acgagccagc gaactatctc ggcctccgtc aggccctctc cgatgtctgg gagcaggaat
     2341 tccctcgcca tcaggcctcc agggtctcct gtgctacccg cacgatccgg tccacggtcg
     2401 gaaggtagtg gccctcggac cgcttcaacg ggaagatggt gtcccagccg gtgacccgcc
     2461 tcaccggcgc cgccagcgag tagagggcct gctcctggac gatggctgcc acctcggctc
     2521 ccaggccggc gtttcggggg gcctcatgaa tcactaccac ccggcccgtc ttggtcgctg
     2581 agcgcacgat gcccccggca tcgatcgggg agagcgtgcg caggtcgagg acttccacgg
     2641 atgccccgga ctcctccaga cgggttgccg ccgccctggt ctccttcatc atcgcgcccc
     2701 aggccaccag ggtgatgtcc tccccctctc gctcgacgcg gagcgggccg atctcggttg
     2761 tgtagtacga ctcgggcacc tcttccttga tcgctcgata gaggcggatc ggttcgagga
     2821 acagcacggg gtcggggtcc tcgatggccg ccagaagcaa cccgcgggcg tcggagggcg
     2881 tcgacggaac gatgactttc aggcccggga tatgggcgta gatcgcctct gtcgactcgg
     2941 aatgatgctc ggcggcgccg attcccccac caaagggcac cctgatca
//
LOCUS       DASTXO010000490         3231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1023466, whole genome shotgun sequence.
ACCESSION   DASTXO010000490 DASTXO010000000
VERSION     DASTXO010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023466"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VFU96_10395"
     CDS             <1..386
                     /locus_tag="VFU96_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEU5113716.1"
                     /translation="EKVIEPLRSAARDHGRPEPMVLATRWSIYAADDEEAWEAIFPWR
                     GLRAPGRLEAVDPADLREMADAMPREEILGQYTRVRSADDIVEAYRPLVRDLDADVVT
                     FQMAALDQPGLIRLLGSEVLPALKG"
     gene            complement(392..1045)
                     /locus_tag="VFU96_10400"
     CDS             complement(392..1045)
                     /locus_tag="VFU96_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113717.1"
                     /translation="MRRALLVCSLAVVACSGGGATTTVTIGEGASDPVGAIEELQSLL
                     AQGDFAAASSLAIPGQAGLASLAEGATPSAVAATLEDGDEEVAANFWNGFAQGVGETF
                     VGEVAVEDLGTTNEEGVQFFVVGVTPEGGAQRLMVTRDVDGQRVDLFATFGAGLAEGL
                     MGPVELLLGSSNEDSRLILSALQDVVPSLLVAANDESLSPDATQRILQLVELITRVG"
     gene            complement(1038..1577)
                     /locus_tag="VFU96_10405"
     CDS             complement(1038..1577)
                     /locus_tag="VFU96_10405"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEU5113718.1"
                     /translation="MLFWHLGATLWLFRWIFRDPKVDVRFLLAGAILPDVADLVIGTG
                     IMGGSLAIGELWFHTLLAPTLYMATVLLFTRRGRQRRAYMALGVGWLFHILLDGLWTD
                     PEVLFWPFFGWELPAGIPPFWPQAWERAMNDPWRWVLDAVGLGYLIWLWFATGLNRRE
                     RRDSFWRTGRIPDMVGQDA"
     gene            complement(1581..2282)
                     /locus_tag="VFU96_10410"
     CDS             complement(1581..2282)
                     /locus_tag="VFU96_10410"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEU5113719.1"
                     /translation="MTSLADLLDVQELDLQIDRLLARRQSLPELDAYKHAHEREQDLT
                     AEILTAAGELKTLELEFDKSEGELQMLEAKLTEHETRLFAGGMSARETEHMRLEVQSL
                     HGQQEALEERVLAMLERLDPAREHVAGLEARRDATTEEKAGYEAVIKSEWKQIDAELA
                     RKEERKTEALAPVPDDLVEMYETLRRSKEGVAIGRFEHGVCGGCHMTLSPTEQVEAFE
                     ADIPRCVHCRRILVA"
     gene            2348..>3231
                     /locus_tag="VFU96_10415"
     CDS             2348..>3231
                     /locus_tag="VFU96_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HEU5113720.1"
                     /translation="MDLKAPLGWGPPPDWRRVVAIDSHTGGEPFRVVVDGAPVIEGAT
                     MLERRRFAEHHIDDLRRALMWEPRGHADMYGAFVGPPTDPTSDISVLFIHNEGFSTMC
                     GHGIIALCKVAIDTGLLPAQTPETVIRIDTPAGQIEATAQVTNGQVSSVAFRNVPSFV
                     TVMGRTVDVPGLGQVRYDLAFGGAFYAYVAADDVGLSIEPSSISRLIDAGRAIKRVVV
                     DSGPIDHPVEPRLGFLYGVVFVAPPEDPVHHSRNVCIFADGEVDRSPTGTGVSGRLAI
                     HHARGEVSIDEAITIESVL"
BASE COUNT          574 a         1075 c         1011 g          571 t
ORIGIN      
        1 cggagaaagt catcgaaccc ctgagatcgg cggcccggga ccacgggcga cccgagccga
       61 tggtgctcgc cacccgttgg tcgatctacg cagctgacga cgaggaggca tgggaggcga
      121 tcttcccatg gcgagggctg agggcgccgg gcagattgga ggcagtcgac ccggccgact
      181 tgcgggaaat ggccgacgct atgccccgcg aggagatact gggccaatac accagggtgc
      241 gttcggcaga tgacatagtc gaggcctatc gacccctggt tcgggatctc gatgccgacg
      301 tggtcacttt ccagatggca gccctcgacc agccgggcct catccgtctg ctgggaagcg
      361 aggtcctgcc ggcgctgaag ggctgagtcg ttcagccgac ccgcgtgatc aactccacga
      421 gttgcagtat ccgctgcgtt gcatctggtg acaagctctc gtcgttggcc gcaaccagca
      481 gagaaggcac cacgtcttgg agcgcgctca gtatgaggcg cgagtcttcg ttggacgacc
      541 cgagcaacaa ctccaccggg cccatcagac cctctgccag cccggcgccg aacgtggcga
      601 agagatcgac ccgctggcca tcgacgtccc gggtgaccat gagccgctga gcgccccctt
      661 cgggcgtgac tccgacgacg aagaactgca ctccttcctc gttggtggtg ccaagatcct
      721 cgaccgccac ctccccgacg aaggtctccc cgacaccctg ggcaaacccg ttccagaagt
      781 tggccgccac ctcctcgtcc ccgtcttcca atgtggcagc caccgccgaa ggggtggctc
      841 cttcggcaag cgaggccaat cccgcctgac cggggatcgc caacgagcta gccgcagcga
      901 agtctccctg agcaaggagg ctttgaagct cttcgatggc gccgaccgga tccgatgccc
      961 cctcgccgat cgtgactgtg gtggtggctc ccccacccga gcaagccacg accgccagcg
     1021 agcatacgag gagcgctcta cgcatcctgg cccaccatgt cgggtatgcg cccggtgcgc
     1081 caaaagctat cgcgacgctc cctgcgattg agtccggtgg cgaaccacag ccagatcaaa
     1141 tagccgagac caaccgcatc gaggacccag cgccaggggt cattcattgc ccgctcccag
     1201 gcttgcggcc agaacggagg gatgcccgcc ggcagctccc agccgaagaa cggccagaac
     1261 aggacttcgg ggtcggtcca caagccatcg agaaggatat ggaacaacca cccgacgcca
     1321 agtgccatgt aggcacggcg ttggcggcca cgtctggtga aaagcagcac ggtggccatg
     1381 tagagagtgg gagcgaggag ggtgtggaac cagagctcgc ctatggcgag ggatccgccc
     1441 atgatcccgg tgccgataac caggtcggcc acgtcgggga ggatcgcccc ggccaggagg
     1501 aacctcacgt ccaccttcgg atcccggaaa atccatcgga acaaccacag ggttgccccg
     1561 aggtgccaga agagcatggc tcaggcgacg aggatccgcc gacaatgaac gcatctgggg
     1621 atgtcggctt cgaatgcctc gacctgctca gtgggtgaca gcgtcatgtg acagcccccg
     1681 cataccccat gttcgaatcg accgatggcc accccctcct tcgaacgacg gagtgtctcg
     1741 tacatctcga cgaggtcgtc gggtaccgga gcgagggctt ctgtctttcg ctcctccttg
     1801 cgcgccagct cggcgtcgat ctgcttccac tccgacttga tgactgcctc gtaacccgcc
     1861 ttctcctcgg tggtggcgtc acggcgggct tcgagcccgg ccacgtgctc acgagccggg
     1921 tctagccgct ccagcatggc gagcaccctc tcttcgagtg cctcctgttg gccgtggagg
     1981 ctctgtacct cgagccgcat gtgctcggtc tccctggcgc tcatcccacc ggcaaacagc
     2041 ctggtctcat gctcggtcaa cttggcctcg agcatctgaa gctctccttc ggatttgtcg
     2101 aactcgagct cgagggtctt caactcgccg gccgcggtca ggatctcggc cgtgagatcc
     2161 tgttcgcgct cgtgggcgtg cttgtaggca tcgagctcgg ggagtgattg gcgtctggcg
     2221 agtagacgat cgatctgaag atccagctcc tgcacgtcga gcagatcggc aagactcgtc
     2281 atctccattg gggagggcag ggtagtgcca gggcgtggcc aagcttcccc ctcctaacct
     2341 cacgcccttg gacctgaaag cgccgctggg ttgggggccg ccacctgatt ggcggcgtgt
     2401 cgtggccatc gattctcaca cgggtgggga gccgtttcgc gtcgttgtcg atggcgcacc
     2461 ggtgatcgaa ggggccacca tgctggagcg ccgccggttc gccgagcacc atatcgacga
     2521 ccttcgtcgc gccttgatgt gggagccacg gggccatgcc gacatgtatg gcgccttcgt
     2581 cggaccgccg acggacccaa cgagtgacat ctcggtcctg ttcatccaca acgagggctt
     2641 ctccacgatg tgcggccacg gcatcatcgc cctctgcaag gtcgcgatcg acaccgggct
     2701 gcttcccgcc caaacgccgg agaccgtgat caggatcgac actcccgcgg gccagatcga
     2761 ggcgactgcg caagtcacca acggccaggt gagctcggtc gccttccgca acgtgccctc
     2821 gttcgtcacc gtgatgggcc gaacggtgga cgtgccaggc ctgggacagg ttcgatacga
     2881 cctggccttc gggggtgctt tctatgccta cgttgctgcg gacgacgtgg ggctgtcgat
     2941 cgagccttcg agcatctccc ggctcatcga cgcgggtcgg gccatcaagc gggtagtagt
     3001 cgattcaggg ccgatcgatc atccggtcga gccccggctc ggctttctct acggggtcgt
     3061 ctttgtcgct ccccccgagg acccagtcca tcactcccgc aacgtctgca tcttcgccga
     3121 tggggaggtg gaccgctctc ccaccggcac cggggtcagt ggccgtctcg ccatccatca
     3181 tgcccggggt gaggtctcga tcgacgaggc gatcaccatc gaaagcgtcc t
//
LOCUS       DASTXO010000491         3764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1797703, whole genome shotgun sequence.
ACCESSION   DASTXO010000491 DASTXO010000000
VERSION     DASTXO010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1797703"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="VFU96_10420"
     CDS             <1..846
                     /locus_tag="VFU96_10420"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5113721.1"
                     /translation="GRIVGVRSRQVDWVVVADQTIGRTIQEPGWRWSTHIRPIVGGEW
                     CQSRHVGMVVSGRMHIVSDDGDEFDLGPYDVIDVAPGHDAWVLGDEPLISIEWSGVRG
                     WLEPLESLNERVLATVVVTDIVDSTGHVTRLGGSRWNDLLARHNSRMRDVVTTFRGRE
                     IKSTGDGFLVVFDGAARAIRCARRMVDSATEDGISIRAAVHTGEIEFVGDDVLGVTVH
                     EATRVAALAAEGEVLVTAVTRDLAAGVGLSFEDRGDHDLRGFDGVRRLFAVGGASSPL
                     GVPVT"
     gene            843..1145
                     /locus_tag="VFU96_10425"
     CDS             843..1145
                     /locus_tag="VFU96_10425"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5113722.1"
                     /translation="MSVRTDPEGNETKALFALVDFGGAEVLEIGCGDGRLTWRYAERA
                     AHVTAIEPFGGSIARAKERLRETHFPVEFRHANLEDFAADTDADVFDIALLSWSLC"
     gene            1161..1607
                     /locus_tag="VFU96_10430"
     CDS             1161..1607
                     /locus_tag="VFU96_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113723.1"
                     /translation="MVYALEEIHRLLKPAGALIEIHPSMEGPPLVEVRSNGRLSFAED
                     DPGFDYAEDVRQAEAAVASVVDQGMFVLEDRRRFELRTYADSVKELRDYWEVAGAYDP
                     EEKEETLVRRRDEMYERAHAGMEDAARPSEIVYVEPATMSRLTASR"
     gene            1935..2456
                     /locus_tag="VFU96_10435"
     CDS             1935..2456
                     /locus_tag="VFU96_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020501449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU5113724.1"
                     /translation="MPSDEEITFADHAGRFYARRYGMAPMMGRLLGYLGVCEPREQSI
                     TELADALLASRSAIAGAVSALETLGLVQRSRAAGERMDRVWIDMSSPRTTGFDLAEYQ
                     EQRELAQEGLHLLADAPPERRAILLEWAALLDFLLERLPVLEQEWKERREALQAAGEL
                     PVVPGSNRERRAR"
     gene            2639..3601
                     /locus_tag="VFU96_10440"
     CDS             2639..3601
                     /locus_tag="VFU96_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020491533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5113725.1"
                     /translation="MRAMDGTANAIEVRNLVKGYKGVPVLDDASFTVRRGTVFALLGA
                     NGSGKTTTINVLTTLIKADGGTATVAGFDVAEQPSKVREQISVTGQSAAVDDVLTARE
                     NLVLMGELRHIADPGQTATDLLDKFELNEAADRRLLTFSGGERRRLDIAMSLVGDSPI
                     IFFDEPTTGLDPMGRTDMWLTIRQLVENGTTVFLTTQYLEEADRLADEIAILHGGRIV
                     ASGTPEELKAMVPTGLVELEFDDEAQLSAALRALGEQHEVDRVDSKLVVTTTGSVAEV
                     ADMFIRLKDRGIEPTGFSRQLPTLDDVFFKFLGEEGEDRLASSH"
     gene            3612..>3764
                     /locus_tag="VFU96_10445"
     CDS             3612..>3764
                     /locus_tag="VFU96_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5113726.1"
                     /translation="MTKRSLRHTTRSPDTIITVVVTPIALLLLFVYVFGGALGEQTGS
                     IEYVDFI"
BASE COUNT          663 a         1151 c         1343 g          607 t
ORIGIN      
        1 ggacgaatcg ttggcgttcg atcacggcag gtcgactggg tggtggtcgc ggaccagacg
       61 atcggccgca cgatccagga gccgggctgg agatggtcga cgcacatccg accgatcgtc
      121 gggggggagt ggtgtcagag ccgacacgtc ggcatggtcg tctcggggcg gatgcacatc
      181 gtctcggacg acggggatga gttcgacctc ggaccctacg acgtgatcga cgtcgcgccg
      241 ggccacgacg cgtgggtcct gggcgacgaa ccgttgatca gcatcgagtg gagcggggtt
      301 cggggatggc tcgagccgct cgagtcgctg aacgagcgcg tcctcgctac ggtggtcgtg
      361 accgacatcg tcgattcgac cgggcacgtg acccgcctgg gggggagcag gtggaacgat
      421 ctcctcgcgc gtcacaattc ccggatgcgg gacgtcgtga cgacgttccg tggccgcgag
      481 atcaagtcga cgggagatgg gttcctggtc gtcttcgacg gtgcggcgcg ggcgatccgt
      541 tgcgccagga ggatggtgga ttcggcgacc gaggacggga tctcgatccg ggccgcggtc
      601 catacgggcg agatcgagtt cgtcggcgac gacgtgctcg gcgtgacggt ccacgaagcg
      661 actcgggtgg cagcgttggc ggcggaggga gaggtactcg tgacggctgt aactcgggac
      721 ctggcggccg gggtcggact gtcgtttgag gatcgaggag atcacgacct ccgagggttc
      781 gacggtgttc gtcgactgtt cgccgtcggg ggagcgagct cacccctggg ggttcccgtg
      841 acgtgagcgt tcggaccgac cccgagggca acgagaccaa ggcgctgttc gcgctggtcg
      901 atttcggtgg cgcggaggtc cttgagatcg ggtgcggaga cggacgcctc acgtggcgct
      961 acgcggaacg cgccgctcac gtgacggcga tcgagccgtt cgggggttcg atcgccaggg
     1021 cgaaggagcg gctgcgtgag acgcacttcc cggtcgagtt ccgccacgcc aatcttgagg
     1081 acttcgcggc cgacaccgac gccgacgtct tcgacatcgc gctcctctcc tggtcactct
     1141 gctgaatgga gcacgagggc atggtctatg ccctggagga gatccaccgg ctactgaagc
     1201 cggccggcgc cctgatcgag atccacccct ccatggaggg accgccgctc gtggaggtga
     1261 ggtcgaacgg acggctgtcc ttcgccgagg acgatccggg gttcgactac gcggaggacg
     1321 tccggcaggc tgaggcggcg gtggcgagcg tcgttgacca aggcatgttc gtcctcgagg
     1381 accgacgccg gttcgagctc cggacgtacg cggattcggt caaggagctc cgggactatt
     1441 gggaggtcgc tggtgcctac gatccagagg agaaggagga gacgctcgtg cgtcgacggg
     1501 acgagatgta cgagcgcgcc catgccggga tggaggatgc agcacggcca tccgagatcg
     1561 tctacgtcga gccggcgacg atgtcgcgcc tgacggcgtc ccggtagccg gagacctcaa
     1621 caaccccatc cggcctccgc cggcgcgagt tcatcccgga tgcgggacac accgagcgac
     1681 gcatgcggac cgcacggata gcgcccgaca cgccatggtt ggacgtgatg gccattcgga
     1741 aggacggtgc ctctcagcgg cgtgctccga cgacgagttc atgccggctc gggccgccct
     1801 ccctggtggc cttgccttgg tctgaacgcg tcccgaacgt ggcgagccga tcgagagatg
     1861 actggcgggt gaatgaccaa cactgagata ccagggattt gtgttatctc aagagtttgt
     1921 gtagagtgct ccgcgtgcca agtgacgagg agatcacgtt cgccgaccac gccggtcgtt
     1981 tctacgcccg ccgctacgga atggccccga tgatgggacg ccttctcgga tacctggggg
     2041 tctgcgagcc ccgggagcag agcatcaccg agctggccga tgcgctgctg gcgagtagga
     2101 gcgcgatcgc cggcgcggtt agcgccctgg agacactggg gctcgtccag cggtcccggg
     2161 cggcgggtga gcggatggac cgggtgtgga tcgacatgtc gtcgccccgg acgacgggat
     2221 tcgacctcgc cgagtaccag gagcagcgcg agctggccca agaaggcctg cacctgctgg
     2281 ccgacgcccc ccccgaacgg cgagcgattt tgttggagtg ggccgcgctc ttggatttcc
     2341 tgttggagcg gcttcccgtg ctggagcagg agtggaagga gcggcgggag gcgctgcaag
     2401 ccgccggcga actcccagtg gtgccgggct ccaatcgaga gcgcagagca cgatgacggg
     2461 ccagacaagc ccgcagggga tcgtggccca ggggacgtat gcaggcgggt cgacaacacc
     2521 cacagctccg ccgacgaccc cgaaccgtcg gccaagatcg tccaggtccg cgcccgacac
     2581 agttcggcga cgtcctgatc gcacgctcgt cagttccgaa tcggcaagga gatagctgat
     2641 gagagccatg gacggcaccg ccaatgccat cgaggtgagg aacctcgtga agggatacaa
     2701 gggagtacca gtgctcgacg acgcctcgtt caccgtcagg cggggcacgg tcttcgctct
     2761 gctcggggcc aacgggtctg gcaagacgac cacgatcaac gtcctgacca cgctgatcaa
     2821 ggccgacggg ggcacggcga ccgtcgccgg gttcgacgtg gcggagcaac cctccaaggt
     2881 gcgcgagcag atcagcgtga ccggacagtc cgccgcggtg gacgatgtgc tcaccgcccg
     2941 ggagaacctg gtgctgatgg gcgagttgcg ccacatagcc gatcccgggc agaccgcgac
     3001 ggatctcctg gacaagttcg aactgaacga ggctgccgac cgccgccttc tgaccttctc
     3061 gggtggggag cggcgccggc tcgatatcgc catgagcctg gtgggcgact cacccatcat
     3121 cttcttcgac gagccgacca ccgggctcga cccaatgggc cggaccgaca tgtggctgac
     3181 catccgccaa ctggtcgaga acgggaccac ggtcttcctg accacccagt acctggagga
     3241 ggccgaccgt ctcgccgacg agatcgccat cctccatggc ggcaggatcg tggccagcgg
     3301 cacaccggag gagctgaagg ccatggtgcc aaccgggctc gtcgagctgg aattcgacga
     3361 cgaagcacaa ttgtccgcgg cgctgagagc gctcggcgag cagcacgagg tcgaccgggt
     3421 ggactcgaaa ctggtggtca ccaccaccgg ctcggtggcc gaggtggcgg acatgttcat
     3481 ccggctcaaa gatcgcggga tcgagccgac aggattctct cggcagctcc cgacgctgga
     3541 cgacgtgttc ttcaagttcc tcggcgaaga gggggaggat cgacttgcga gctctcactg
     3601 acacctggac catgacgaag cgaagcctcc gtcatacgac gcgcagcccc gacacgatca
     3661 tcacggtcgt cgtgacaccg atcgccctgt tgctgttgtt cgtctacgtg ttcggtggcg
     3721 cactcgggga gcagaccggc tcgatcgagt acgtcgactt catc
//
LOCUS       DASTXO010000492         4894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_582088, whole genome shotgun sequence.
ACCESSION   DASTXO010000492 DASTXO010000000
VERSION     DASTXO010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582088"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VFU96_10450"
     CDS             <1..795
                     /locus_tag="VFU96_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU5113727.1"
                     /translation="YIGQEANAVGILDHVEEGDSVFSNHRCHGHFLAYGGDMRGLFAE
                     LMGKATGICGGRGGSQHIHWRRFFSNGVLGSTIPVATGVGLAGRFRGDNDIVVVFMGD
                     GALGEGVIYEALNMAALWRAPVLYVVENNRIAQTTPIEMAMAGSIPARFEAFGVPARS
                     IASSDVDEVSLTASEAIAEVRDGGTPRALIIDTNRFAPHSKSDDTRPEEAIEAMRTTS
                     DPIEIQAPRLDHTERETVAAEVAEDVSRAFAAAIADPEPDLEVGAA"
     gene            792..1817
                     /locus_tag="VFU96_10455"
     CDS             792..1817
                     /locus_tag="VFU96_10455"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEU5113728.1"
                     /translation="MSTVLESLNSGLHAAMEADESVYILGEDVLDPYGGAFKVTRGLS
                     TAYPDRVITTPVSEAGFVGVATGLALSGLRPVVEIMFGDFVTLITDQVINGLSKYGWM
                     YGGALEIPVVIRTPMGGRRGYGPTHSQTLEKLFLGVPGLRVLAPSAFTDPGALLRAAI
                     DDPGPVLFVENKLQYQRRLDDPAFTALEARVQTQEGYASTATVSVAGAPRPQLTIAGY
                     GDMAERAAEAQLKMAYELEIFSELVVLEQLDPLGPGGDLPRPLADSLQQTGCLLTVEE
                     GTHTHGWGAEVVARAVEASAQKLMIARVAAFDSPVPASRTLESSVLPDVDVMVGAAQK
                     LVAALDG"
     gene            1810..2886
                     /locus_tag="VFU96_10460"
     CDS             1810..2886
                     /locus_tag="VFU96_10460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HEU5113729.1"
                     /translation="MADPVVVEVPLVNPNEPDALVVDIAVPDGGRVSAGDVLCSLETS
                     KSVEDVYAEVSGYVFGLAAKLGSTVTAGHVLCYLSDDPQWTAPVDDGGDQGDDVPEDL
                     RITEPALAVARELGLDLSTLPGGVLITERHVREAADIGGAEAPPADPKSTIIFGAGGH
                     GKTLIELIRSVGGLEPVGVVDDRLPRGSEVMGVPVLGSRDDLGSIRRDGIGLAVNAIG
                     GISNLASRVEVSRALAASGFVLPALVHPTAFVEASASIGAGAQILAHSYVGSDAVVGD
                     GVIVNTGAVVSHDCVLEDHVNLSPGCLLAGGITVGEATLLGMGVTTYLGISIGANVRV
                     GNGAIITAPVPDGRRIATGSVWGG"
     gene            complement(2906..3517)
                     /locus_tag="VFU96_10465"
     CDS             complement(2906..3517)
                     /locus_tag="VFU96_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013006373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEU5113730.1"
                     /translation="MQELVVDFITSLDGYASAEGWPGWWGLQGPEYLAWLGDQPERDY
                     TILMGANTYRLMSGFAAEAEASESEEATEEEVTTAELSRVPKVVFSSTLQPPLTWANT
                     QLVSGDAVEAVGAMKRDGSSPMVTLGSLTLCRSLLEAGLVDRYRVVVFPVITGKTGRE
                     RIYDGYPDVALEMVASQTFDGRIQLLEYVPTLTDGPPETQPAS"
     gene            complement(3549..4703)
                     /locus_tag="VFU96_10470"
     CDS             complement(3549..4703)
                     /locus_tag="VFU96_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113731.1"
                     /translation="MGRAALMARADRRSARLRLLGLVSLLVVTGASVAVAQESQFGGK
                     IQAGGQITISSDETVGGDLYASGGRVLIEGTVDGDLIVAAGQVTISGEVSGDVMASAG
                     AVDISGDIGGDARVASGQSSVSGSIGEDLFVASGQTTITDSGEVGEDLVFGSGRMAMN
                     GTVGGDVLGAAGDYDRQGTVAGTENVTIRDVEAPTVTERVLDALQRFISILLVAALFL
                     WLAPRFMDQPTMVLKQRPLASLGIGVVGVIAFCVLVFLVILVAVLLGIGMGLVGLSDL
                     VGAIIFAAVLAVAVLAFLFFIVIVFGAPAWVGMALGSLALSLDSTARRLAALIIGLIV
                     VVALTSIPIAGRWIGLIVALFGLGAAILAFRPRRTTPATIEPSGVSPQAV"
BASE COUNT          850 a         1558 c         1626 g          860 t
ORIGIN      
        1 tacatcggcc aggaagccaa tgcggtggga atcctcgacc atgtcgagga aggcgattcg
       61 gtgttctcca accaccgctg ccacgggcat tttctcgctt acggcggcga catgcgcggc
      121 ctcttcgccg agctgatggg gaaggccacg ggtatatgcg ggggtcgtgg cggatcccaa
      181 cacattcact ggcgccgctt cttctccaac ggcgtgctcg gctccaccat cccggttgcc
      241 actggagtcg ggctggctgg acggtttcga ggtgacaacg acatcgtcgt ggtcttcatg
      301 ggagacggtg ccctgggtga aggagtgatc tacgaggcgt tgaacatggc ggccttatgg
      361 cgggcgccgg tgctgtatgt ggtggagaac aaccgcatcg cacagaccac accgatcgag
      421 atggccatgg ccgggagtat ccccgcccgc ttcgaggcct tcggcgtgcc cgcccgctcc
      481 atcgcctctt cggacgtcga tgaggtctcg ctgaccgcgt ccgaggcgat cgcagaggtc
      541 cgtgacggcg gcacgcctcg agcgctgatc atcgacacca accgtttcgc acctcattcg
      601 aagagcgacg acacgaggcc cgaggaagcg atcgaggcga tgcggaccac ctcggacccc
      661 atcgagatcc aagccccacg gctcgatcac accgagcgcg agaccgtcgc cgccgaggtc
      721 gccgaggacg tgtcccgtgc tttcgctgcc gccattgccg acccggagcc cgacctggag
      781 gtgggagcgg catgagcacc gtcctcgagt cgctcaactc cggactccat gccgcaatgg
      841 aggccgatga gagtgtgtac atcctcggcg aggacgtcct cgacccctat gggggggcgt
      901 tcaaggtgac ccgcggcctg tcgacggcat acccggatcg ggtcatcacc acgccggtat
      961 cagaggccgg ttttgtcggc gtggccacgg gattggcgct gagcgggctt cgcccggtgg
     1021 tggagatcat gtttggagat ttcgtcacac tgatcaccga ccaggtgatc aacggtctgt
     1081 ccaagtacgg atggatgtac ggcggcgccc tcgaaatacc tgtcgtcatc cgcacaccga
     1141 tgggtggtcg ccgcggatat ggcccaactc acagccaaac cctggagaag ctcttccttg
     1201 gtgttcccgg gttgcgggtg ttggcaccct ccgccttcac ggatccgggt gccctgctgc
     1261 gggcggcgat cgacgatccc gggccggtct tgttcgtgga gaacaaactt caataccaga
     1321 ggcggctcga tgaccccgcc ttcactgccc tcgaggcgcg agtgcagacc caggagggtt
     1381 atgcctccac ggccacggtg agtgtcgccg gggcaccgcg accccaattg accattgccg
     1441 gctatggcga catggcggag agagcggccg aggctcagct gaaaatggcg tacgagctgg
     1501 agatcttctc agagctggtc gttctggagc agttggatcc actgggcccg ggtggggacc
     1561 tccctcgccc tctcgccgac tcgctccagc agacagggtg tctgctcacc gtggaggagg
     1621 gaacccacac ccatgggtgg ggcgccgagg tggtggcgcg ggccgtggag gcgtcggcgc
     1681 agaagttgat gatcgcccgg gtggcagcat tcgacagccc ggtgccggcg tctcggacgc
     1741 tggagtcgtc agtcctgcct gacgtcgacg tcatggtcgg cgccgctcag aagctcgttg
     1801 ccgccctcga tggctgatcc ggtcgtcgtc gaagttccgc tcgtcaatcc caacgagccg
     1861 gatgccctgg tcgtcgacat tgcagtacct gatggcgggc gcgtctcggc cggggatgtc
     1921 ctctgctcac tggagacgtc gaagtcggtc gaggacgtct atgccgaagt gtccgggtac
     1981 gtctttggac ttgccgccaa gttgggttcg acggtgaccg cgggccacgt gctctgctac
     2041 ctgtccgacg acccgcaatg gacggcaccg gtcgatgacg gtggagacca aggcgacgat
     2101 gtcccggagg acctccgcat caccgagccg gcgctggcgg tcgccaggga gctcgggctc
     2161 gatctgagca ctttgccggg aggggtcctg ataaccgagc gccacgttcg tgaggctgct
     2221 gacatcggcg gggcagaggc gccacccgcc gatcccaagt cgacgatcat cttcggtgcc
     2281 ggtggtcacg gcaagacctt gatcgagctc atccggtcgg tcggcggatt ggagcccgta
     2341 ggcgttgtcg acgatcgcct gcctaggggc tcggaggtca tgggcgtccc ggtgctgggg
     2401 agccgtgacg atctgggatc gatacggcgt gacggcatcg gtctggctgt gaacgcgatc
     2461 ggaggcatat ccaatctggc gtctcgggtc gaagtgtccc gtgcgctcgc cgccagtggg
     2521 tttgtcctgc cggcactcgt ccaccccact gcgttcgtcg aggccagcgc ctcgatcggc
     2581 gcgggagccc agatcctggc tcactcctat gtcggctcgg atgcggtagt gggcgatggg
     2641 gtgatcgtca acaccggggc ggtggtatca cacgactgtg tcctcgagga ccacgtcaac
     2701 ctgtcccccg gttgtcttct cgctgggggg atcaccgttg gtgaggccac cttgctgggc
     2761 atgggtgtga cgacctatct cgggataagc atcggcgcca acgtccgggt ggggaatggc
     2821 gcgatcatca cggctccggt gccagatggc cggaggatcg cgaccggctc cgtctggggc
     2881 ggctaacgcg gggccactcc gatcgtcagg aagcgggttg cgtctctggc ggaccgtcgg
     2941 tcagtgtggg gacatattcg agcagctgga ttctgccgtc gaaggtctgg ctggcaacca
     3001 tctccagggc gacatcggga taaccgtcat agatgcgctc gcggccggtc ttcccggtga
     3061 tcacggggaa gaccaccacg cggtagcggt ccacgaggcc agcctcgagg agtgatcggc
     3121 acaaggtgag gctgccgagg gtgaccatcg ggctggagcc gtcccgcttc atggctccca
     3181 cagcctccac tgcatccccg ctgacgagct gggtattggc ccaagtcaat gggggctgga
     3241 gtgtcgagga gaacacgacc ttgggcactc tggagagctc ggctgtggtg acctcttcct
     3301 cggtggcctc ctccgattcg gatgcctcgg cctcggcggc gaatccggac atcagccggt
     3361 aggtgttcgc tcccatcaag atcgtgtagt cgcgttcggg ctggtcgccg agccaggcga
     3421 gatactcggg tccctggagt ccccaccacc cgggccaccc ttctgcggac gcgtagccgt
     3481 cgagtgaggt gatgaagtcc actactagtt cctgcatcgt tctcctcctg gggttacgag
     3541 accttggatt acacggcttg cggtgagacg ccggacggtt cgatggtcgc cggcgtggtc
     3601 ctgcgaggac ggaaggcgag gatcgccgca cccagtccga atagagcgac gatcaggccg
     3661 atccagcggc ccgcgatggg aatggaagtc agagccacga cgacgatgag accgatgatc
     3721 agggcggcca gccggcgagc agtcgagtcc agcgagagcg ccaagcttcc cagcgccatg
     3781 ccgacccaag cgggtgcgcc gaagacgatg acgatgaaga agaggaaggc aagcaccgcc
     3841 acggcgagga cggcggcgaa gatgatcgct ccgaccagat cgctgagacc caccagcccc
     3901 attccgattc ccagcaggac agccaccaga atcacgagga aaacgagaac acaaaatgcg
     3961 atcacaccga cgacaccgat gccaagcgag gccaggggcc gctgcttcag gaccatggtg
     4021 ggttggtcca tgaagcgggg agccagccag aggaagaggg ctgcgaccag caggatgctt
     4081 atgaaccgct gcagtgcgtc caggacccgt tcggtgaccg tgggagcctc gacgtcccgg
     4141 atcgtcacgt tctcggtgcc ggcaactgtg ccctgtcggt cgtagtcgcc tgcggcgccg
     4201 agcacgtcac cgccgaccgt tccgttcatg gccatccgac cactcccgaa gacgaggtcc
     4261 tctcccacct cgcccgaatc ggtgatggtg gtttgtcctg aggcgacgaa caggtcttcc
     4321 ccgatcgatc cgctcaccga tgactggcca gaagccactc tcgcatcgcc cccgatgtct
     4381 ccggagatgt ccacggcccc ggcagacgcc atgacgtcgc cgctcacctc accggagatg
     4441 gtcacctggc cggcagccac gatcagatca ccatccaccg tgccctcgat caggacgcgc
     4501 ccccctgatg catacaggtc cccacccacc gtctcatcgc tcgagatggt gatctggccc
     4561 ccggcctgga tcttgccgcc gaactgggac tcctgtgcca ccgcgaccga agcgccggtc
     4621 acgacgagta gcgagaccaa tcccagcaac ctcaggcgcg cgctgcggcg atcggctctc
     4681 gccatgaggg ccgccctacc catccgccgg cgtcgttaaa cagactcatc cgcgttgggg
     4741 gtatggttgg cacatgacac ggcatgagcc ggtcgacatg gtcccgatgc tttctcgggg
     4801 aaagcatcgg agcccgcgca ggggggcctg cttcatggag ttcgcctcat tcctcgccgg
     4861 cgagcgatgg agcgaccacc ccgattgcac gcat
//
LOCUS       DASTXO010000493         2613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_29700, whole genome shotgun sequence.
ACCESSION   DASTXO010000493 DASTXO010000000
VERSION     DASTXO010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29700"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..888)
                     /locus_tag="VFU96_10475"
     CDS             complement(1..888)
                     /locus_tag="VFU96_10475"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU5113732.1"
                     /translation="MAAILGWGSAAVLTRAVLTSGVSTFVVVPVRFGIALLALVLFSL
                     FWARFRGSDRRHWARGAVLGAIALVMPNTLFTLGLEDLPVNIGGLLIALVPIATVGAA
                     HFLVEGERFNPRSIPGLVTSLVGTGILVGLGGEGLEGVGNLWRGVGLSLIAVVLAGTG
                     GALTRRFALEVGGDGLVVPQFATATALGVIATPFLNTTPLSSIELEQWALLLALGTFA
                     TAIPFTSFIIAAQVNSAARLGVAGYLVPVIAVTLAVAFLGEELTPAVVVGALLIISGV
                     IITERSSRHVPVPGVDIAE"
     gene            978..2015
                     /locus_tag="VFU96_10480"
     CDS             978..2015
                     /locus_tag="VFU96_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113733.1"
                     /translation="MKRLLSLTAVLALALVACAEPSTNPDAMLLSYAMAAGDEFQYEV
                     GLDQHIEMNASGDAALLAGEDVPGEASVDIAGTATFTHAISDGPEPGTFQVHITGEFT
                     DVSVTGTVDGESVDSSDIPDFAAMEPIDVTVVVDEQGNIVDEGADIEDPLSGLLGGLG
                     ALGGGGNPAPGLDPGQFVGPPLPEEEVTVGDSWSDEIETAGPGEDPIVTSIDSTVTGV
                     EELDGAEVLVIDSNTTTSLIEVDLADFFAGMIGGMAPEGASPEETAELEAMLDQLQFL
                     ISVDESTADSTTWFDAEAGLARQSETTAGSSISMDMNIPDEKTGELAGFVMDMTLDQD
                     VTYRLISGPTA"
     gene            complement(2053..>2613)
                     /locus_tag="VFU96_10485"
     CDS             complement(2053..>2613)
                     /locus_tag="VFU96_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113734.1"
                     /translation="LRVASEATGVPLSTLRNWARKGRVSTALEESAEGTHRMVSLEGI
                     RARARQLGHSVVEPGEAATNQVPPPPAAAVFPGDVAAPPPGTMLVPIDAWDKMLLQLG
                     NLHQAGQQLAEARERAARAETEAQFLRERLADLRATPAAAPADAAPILEESPIRPEVE
                     PEPAPPVKLPRFTAYLWRMAASGRRK"
BASE COUNT          474 a          841 c          896 g          402 t
ORIGIN      
        1 tcattcggcg atatcgacgc cgggaaccgg gacgtggcgg ctggagcgct cggtgatgat
       61 caccccgctg atgatcagga gggcgccgac taccacggcg ggagtcagct cttcgcctag
      121 gaaggcaacc gccagtgtca ccgcgatcac cgggaccaga tagccggcca ctccgagacg
      181 ggctgcggag ttgacctggg cggcgatgat gaacgacgtg aaggggatcg ccgtggcgaa
      241 cgtccccaag gcgagaagta gggcccactg ctccaattcg atcgaagaca gcggtgttgt
      301 gttgaggaac ggcgtggcga tgacgcccag ggccgtggcg gtggcgaact ggggcacgac
      361 gagaccgtca ccgcccactt cgagggcgaa tctgcgggtc agggcaccac cggtgccggc
      421 cagcactacc gcgatcagtg agaggccgac ccctcgccac agattgccga cgccctcaag
      481 cccctcaccg cccagcccga cgaggatccc ggttccgacg agtgacgtga ccagtccggg
      541 gatcgagcgc gggttgaatc gctcgccctc cacgaggaaa tgagccgcgc caaccgttgc
      601 gatcgggacc agtgcgatca gcagcccgcc gatgttgacc ggaaggtctt ccagcccgag
      661 tgtgaagagg gtgttgggca tgaccagggc gattgccccg agaacggcac cccttgccca
      721 atgtcggcga tcggagccgc ggaaacgagc ccagaacagg gagaagagca ccagtgccag
      781 caacgcgatg ccgaaccgca ccggcacgac tacgaaggta gaaacgccac tggtcaggac
      841 agcccgggtg aggaccgccg ccgagcccca gccgaggatc gctgccacga gaggggtcca
      901 cggattcacg aggccgggac ggtacaaggc ggtttggggc taaaaggtaa ggccgcggcc
      961 gccgataacg tgtcggtatg aagcgtctcc tgtctctcac cgcggtcctc gcgctcgcac
     1021 tcgtcgcgtg tgccgagcca tccaccaatc ccgatgccat gctcctctcc tacgccatgg
     1081 ccgccgggga cgagtttcaa tacgaggtgg gtctcgacca gcacatcgag atgaatgcct
     1141 ccggtgacgc cgccctgctg gcgggcgagg atgtcccggg cgaggcctcg gtcgacatcg
     1201 ccggaaccgc cacgttcaca catgcgatct ccgacgggcc cgagccgggg acgttccagg
     1261 tgcacatcac cggcgagttc accgacgtga gcgtcaccgg gaccgtcgac ggcgaatccg
     1321 tcgattccag cgacatcccc gatttcgcgg ccatggaacc gatcgacgtg accgtcgtcg
     1381 tggacgagca gggcaacatc gtcgatgaag gcgccgacat cgaggatccg ctgagcgggc
     1441 ttctgggtgg gctcggggca ctgggtggtg gcggaaaccc ggcacccggg ctggaccctg
     1501 gccagttcgt cggcccccca ctccccgagg aggaggtgac ggtgggcgac tcctggtccg
     1561 atgagataga gaccgccggt cccggcgagg acccgattgt gacgagcatc gacagcacag
     1621 tgaccggtgt cgaggagttg gacggggccg aggttctcgt catcgacagc aatacgacca
     1681 cgtcattgat cgaagtcgat ctcgccgatt tcttcgccgg catgatcggc ggcatggctc
     1741 cggagggggc ttcgccggag gaaaccgccg aattggaggc gatgttggat caactccagt
     1801 tcctgatcag cgtcgacgag agcaccgccg acagcacgac ctggttcgac gccgaggccg
     1861 gcttggccag gcagtcggag accacggccg gcagcagcat ctcgatggac atgaacattc
     1921 ccgacgagaa gaccggggag ttggccggct tcgtcatgga catgactctc gatcaggacg
     1981 tcacctaccg tctgatcagc ggccccacgg cctgaatcgc aatcggccgg ggctttcgtg
     2041 tcgcggtagt tgctacttgc ggcggccgga cgccgccatt cgccacagat aggcggtgaa
     2101 tctgggcagt ttgaccggtg gtgccggctc cggctcaacc tcgggcctga tcggcgactc
     2161 ttcgagtatt ggggccgcgt ccgccggcgc cgcagcggga gttgctctca ggtcggcgag
     2221 acgctcgcgg aggaactggg cttcggtctc ggcgcgggcg gcacgttctc tcgcctccgc
     2281 cagttgctgt ccggcctggt gcaggtttcc cagctggagg agcatcttgt cccaggcgtc
     2341 gatcgggacc agcatcgtgc ccgggggtgg cgcggccacg tctccaggga acacggccgc
     2401 agccggcggt ggtgggacct gattcgtcgc cgcctcccct ggctcgacga cggagtggcc
     2461 cagctgccgg gcacgcgccc tgatcccctc cagcgagacc attcggtggg tgccctcggc
     2521 cgactcttcg agagcagtcg acacccgccc cttgcgagcc cagttgcgaa gggtggaaag
     2581 ggggacaccc gtggcttcgc tcgccacacg aag
//
LOCUS       DASTXO010000494         2089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1282360, whole genome shotgun sequence.
ACCESSION   DASTXO010000494 DASTXO010000000
VERSION     DASTXO010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282360"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..1849
                     /locus_tag="VFU96_10490"
     CDS             320..1849
                     /locus_tag="VFU96_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5113735.1"
                     /translation="MAMFVLVVDTTLMNVSISAVVEDLGTTVSDVQAAIALEALVSAA
                     FILISSKIGDLIGRKRAYVLGLLGYAAGALTMTLTDSLGPMIVFWAIFGGLGASLLLP
                     AMQSLIHGNFSGDAQKRVYALVGASAAIAAAVGPLLGGFVTTFLSWRVGFLLEVVVIA
                     IVLLGMGMVRDVPFTGDRRLDLVGAFFSVLGMGGLVLGILVWQEGGGYVGLLIVLGLV
                     GMFLLARWLLRRKRSGKVTLLDPDLLRSKMFRLGITGQMLQQIALGGSMIVVPIFLQM
                     VLGYNALETGLTIAPLSLTMFGVSVLAGKRAGRRRPAAIIRAGFALLALGVLVMIPIV
                     PRTDTGWWLVIPLFIMGIGLGPLVSQLNNYALAPISEERISEAAGVNSAAGSFGLSFG
                     LAFAGGVMLAVLSLTFVNMAESSIVLDPTQQEQVATALEEDAQLMSTAQLEELVDGQP
                     ETVQDEIIRINTEARPIALQVALLVPLLAGLVGVANGFRMMRLPDVTPSADIEGVALG
                     G"
BASE COUNT          338 a          647 c          689 g          415 t
ORIGIN      
        1 acccaagctg ccctgaccac agagttggct tccacctctc tcgcagagat ggctgcgatc
       61 gacgccgcta tcgaggcggg ttcgtatcag cccgaggcac ctccccatgt tcggtccacg
      121 gagcgacacg gcaaccgcag cgagatcaag cgaggctgat tgacgcccgc gatcgcgcca
      181 ttgcaagcgt tagattcctc gcgtttcccg acggattccg ccggcggggc gatcaggagg
      241 gagcgacttg tcggactcat ccagccctgc ggccaccggg gaaggcgcct ggaacccaag
      301 gctggccatc ttgctggcaa tggcgatgtt cgtcctcgtt gtcgacacga ctttgatgaa
      361 cgtctccatc tcggccgtgg tcgaagactt ggggaccacg gtcagtgacg tccaagcggc
      421 gatcgcgctc gaagcgctcg tgtcggcggc attcatcctg atcagcagca agatcggcga
      481 cttgataggc aggaaacggg catacgtgct ggggctgctc ggatatgccg ccggcgctct
      541 caccatgacc ctgaccgaca gccttgggcc gatgatcgtc ttctgggcga tcttcggcgg
      601 actgggcgct tccctgctgc tacccgcaat gcagtcgctg atccacggca acttctccgg
      661 tgacgcccag aagcgggtct acgcgctggt cggtgcctca gccgcgatcg ccgccgccgt
      721 ggggccactc ctcggcggtt ttgtcacgac attcctctca tggcgggtcg gattcctcct
      781 cgaggtggtg gtcatcgcca tcgtcctgct cggaatgggg atggttcgcg acgtcccctt
      841 caccggtgat cggagactgg acctggtggg tgctttcttc tcggtgttgg gcatgggtgg
      901 cttggtgctc gggatcctcg tctggcagga gggcggcggg tatgtcggcc tcctcatcgt
      961 gctcggcctg gtcgggatgt ttctcttggc ccgttggcta ctccgccgaa agcgatcggg
     1021 caaggtgacc ctgctcgacc cggatctcct caggtcgaag atgttccgtc tcgggataac
     1081 gggtcagatg ctgcagcaga tcgcccttgg cggctcgatg atcgtggtcc cgatctttct
     1141 gcagatggtg ctcggataca acgccctcga aaccggtctc accatcgctc cgctgtcgtt
     1201 gaccatgttc ggcgtgtcgg tcctggccgg aaagcgggcc gggaggcgac gaccggcagc
     1261 gatcatccga gccggcttcg cattgctggc gctgggagtg ctggtgatga tcccgatcgt
     1321 gcctcgcacc gacaccggat ggtggctggt gatcccgctc ttcatcatgg ggatcggtct
     1381 cggtccgctc gtatcgcagc tgaacaacta cgccctggcc ccgatctcag aggagcggat
     1441 cagcgaggct gcgggcgtca actcggcggc cggttccttc ggtctgtcct tcggccttgc
     1501 cttcgccggt ggtgtcatgc tcgctgtcct ctcgctcacc ttcgtcaaca tggccgagag
     1561 cagcatcgtt ctcgacccca ctcagcaaga gcaggtcgca accgctctcg aggaggacgc
     1621 ccagctgatg agcacggcac aactcgagga attggtcgat ggccagccgg agacggtcca
     1681 ggacgagatc atccggatca acaccgaggc gcgcccgatc gcactgcaag tcgccctgct
     1741 ggtccccctg ctcgccggcc tcgtgggggt ggccaatggg ttccggatga tgcgtctgcc
     1801 cgatgtcacg ccttcggccg acatcgaggg cgtggcactt ggaggctgag gccgggcgcg
     1861 agatgaccag ggtccaggaa ccgaaaggat ccggctcgag ccttcatcgg gggggaggca
     1921 gcggcgaata ggatcgctga aatgagcgaa aatcgggtgt tattgaaggg tggacgggtc
     1981 atttcgatgg acccgtccat cggtgagctg ccccaggccg acgtcctgat cgaggagggc
     2041 aaggtcgcga atgtcggcga gacagtcgag gcgcctgacg ccgacgtgt
//
LOCUS       DASTXO010000495         2808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1024134, whole genome shotgun sequence.
ACCESSION   DASTXO010000495 DASTXO010000000
VERSION     DASTXO010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024134"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..116
                     /locus_tag="VFU96_10495"
     CDS             <1..116
                     /locus_tag="VFU96_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEU5113736.1"
                     /translation="SPLGGALLGASTGDEVSYQAPAGTFFVTIKAVRPYEG"
     gene            153..1556
                     /gene="mgtE"
                     /locus_tag="VFU96_10500"
     CDS             153..1556
                     /gene="mgtE"
                     /locus_tag="VFU96_10500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEU5113737.1"
                     /translation="MRLRLRRPRQLARDLLAAARRDHEEVADYLETHGEEWEALAEAA
                     PGDAADVLEQLTEENAAGLLAELEPRDVADILEQIAPELAAELVEDVPLEDLTAAVGE
                     MTGESAADLLYELDDDVTESVLALLDDESEQELRRLLEYPSDTAGGLMTTEIAALPLG
                     LTAGEAIERIRQLHEQYEDFSYVYVVDDEQKLRGVIFFRDLVFARPGASLEDVMVPDP
                     ISVEAMTDREEVAELAQRYRLFGIPVVDDQGRLVGVVTTDAVIEAIQDEATEDFAAAV
                     GAGLGETVYTDVTKSFRSRAPWLILNLILATFVAWVIEQQTSIISSEPVLAALMPIVA
                     TLGGNGGNQSLAVMIRSLASDGVPRAQVSSILLRQAGVGVLNGMLLAVAAAGVTYVLV
                     DSGVFVSNSDPLTVAIVIGIACLANLLIATLSGSAIPLVLRRLGLDPALASSILLTLI
                     TDAVGFGGFLLVAAALL"
     gene            complement(1578..2096)
                     /locus_tag="VFU96_10505"
     CDS             complement(1578..2096)
                     /locus_tag="VFU96_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113738.1"
                     /translation="MSSRALRFSLSGLLMLALAACDDGETTDLSTTSSLIASPTTAGE
                     VATTTTVAPGGETTSTTLVGQPVASHEVVARVSEPAGETLFIVIPEGAYTDVDIENFV
                     VGLVDSGEITYGAEIFDDPGAVDAYRKPEAERTEGETQLIDQHHFASVQNGTTVVFRG
                     PFADSGEFVIGS"
     gene            complement(2221..2553)
                     /locus_tag="VFU96_10510"
     CDS             complement(2221..2553)
                     /locus_tag="VFU96_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113739.1"
                     /translation="MVVSGGDVVVVGGVVVVVDGAVVVLGGWVVVVSSGADVLVVSSG
                     SVVVVDSGVVVVLVSAGSVVVGAVVVVGLGGTVVGGGNVVGGKRMSIVTGGHISAAAT
                     GLAGVVAK"
     gene            2597..>2808
                     /locus_tag="VFU96_10515"
     CDS             2597..>2808
                     /locus_tag="VFU96_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113740.1"
                     /translation="MAWPLSGVGQIGSPFGADRDGGARKHEGNDIAAHRLTPVVAVAD
                     GVLTRIRQERGTDNCCWAIVEHTDGW"
BASE COUNT          486 a          981 c          873 g          468 t
ORIGIN      
        1 cgagcccatt gggtggcgcc ctgctcgggg cgtcgaccgg agacgaagtc tcctatcaag
       61 cgcccgcggg caccttcttt gtcaccatca aagcggtccg cccctacgag ggatagttac
      121 cctcaggccg gttcgccgtt cgaggggtgc cggtgagact cagactcaga aggccgcgcc
      181 agcttgccag ggatctcctg gctgccgcgc gccgtgatca cgaggaagtc gccgactatc
      241 tcgagacaca cggcgaggag tgggaggcgc tggccgaagc tgcccccggt gacgccgccg
      301 acgtcctcga gcagctgacc gaggagaacg cggcaggtct tctcgctgag ctggagccgc
      361 gagacgtcgc cgacatcctg gagcagatcg ccccggagct ggccgcagag ctggtcgagg
      421 atgtccccct cgaggatctc accgctgcag tgggagagat gaccggcgaa tccgctgccg
      481 acctcctgta cgagctcgac gacgacgtga ccgagagtgt gctcgcgttg ctcgacgacg
      541 agtccgagca ggagttacgc cgcctcctcg agtacccgtc ggacacggcc ggtggcctga
      601 tgactaccga gatcgccgca ctgcccctcg gcctcaccgc aggcgaggca atcgagagaa
      661 tccggcagct ccacgagcag tacgaggact tctcctacgt gtacgtggtc gatgatgaac
      721 agaagctgag aggtgtcatc ttcttccgag acctggtgtt cgctcgtccc ggggcaagcc
      781 tcgaggacgt gatggtcccc gacccgatct ccgtcgaagc gatgaccgac agggaggaag
      841 tcgccgaact ggcccagagg tatcgcctgt tcgggatccc cgtggtcgac gatcagggac
      901 gcctcgtcgg agtggtcacc accgatgccg tcatcgaggc gatccaggac gaggcaaccg
      961 aagacttcgc cgccgcagtt ggcgccggtc tcggggagac cgtctacacc gatgtgacca
     1021 agtcgtttcg ctcccgagcc ccatggctca ttctcaacct gatcctggcg accttcgtgg
     1081 cctgggtgat cgagcagcag acgagcatca tctcgagcga gccggtgctc gctgccctga
     1141 tgccaatcgt ggccacactg gggggaaatg gcggaaatca gagtctggcg gtgatgatcc
     1201 ggtcgctggc ctccgacggg gtcccccggg cgcaggtgtc ctccatcctg cttcggcaag
     1261 ccggcgtcgg cgtcctcaac ggcatgctcc tcgcggtcgc cgccgccgga gtcacctacg
     1321 ttctggtcga ctccggggtc ttcgtgagca actcagaccc cctgacggtt gccatcgtga
     1381 taggaatcgc ctgcctggcc aatctgctca tcgccaccct gtcgggatcg gccatccccc
     1441 tcgtcctgcg tcgtcttggc ctggacccgg ccttggcatc gtcgatcctc ctcacgctga
     1501 tcaccgatgc ggtcggcttc ggcggattcc tgttggtggc cgccgccctg ctgtaagacg
     1561 gtcttgctcg ccgggtacta gctcccgatc acgaactcgc ccgagtcggc gaagggaccg
     1621 cggaagacga cggtggtccc gttctgaaca ctggcgaaat ggtgctgatc gatgagctgg
     1681 gtctcccctt cggtgcgctc cgcctcaggc ttgcggtagg cgtcgactgc ccccgggtcg
     1741 tcgaagatct cggcgccgta cgtgatctcg ccactgtcga ccagccccac gacgaagttc
     1801 tcgatgtcga cgtcggtata cgccccttca gggatcacga tgaagagcgt ctcccccgcc
     1861 ggctccgata ctcgggccac cacctcgtgg ctggcgaccg gctgaccaac gagtgtggtc
     1921 gacgtcgtct cgcccccggg tgccactgtc gtcgtcgtgg ccacctcacc ggcagtggtc
     1981 gggctggcga tgagagacga ggtcgtgctg agatccgtgg tctcgccgtc gtcacaggcc
     2041 gccagcgcca gcatgagaag cccggacagg ctgaaacgga gtgctctgga ggacatcatg
     2101 gcctacaaac gtacccgcct ccctcctggt tccaaaccag gcccaggacg gtaaggtcgt
     2161 atcgatgttc ggcagacggg tggccgcctt ctcggtcgcg tccgccctga tcacagggct
     2221 ctacttcgcg actacgccgg ccaacccggt cgctgcagcg gagatgtgcc ctccggtgac
     2281 gatggacatc ctcttcccgc cgaccacgtt gccaccgccg acgaccgtcc caccgaggcc
     2341 caccaccacc accgctccga ccacgacgct ccccgcggag accagcacga ccacaacacc
     2401 cgagtccact acgacgacgg agcctgaaga gacgacgagc acgtctgccc ccgacgagac
     2461 caccaccacc cagccgccaa gcacgactac tgctccatcg accaccacga cgactccgcc
     2521 gacaaccacg acatctccgc cggagaccac caccacccaa ccgccgccgc ccccctgcga
     2581 gccatttgtc tacgacatgg cctggcctct gtccggagtc ggccagatcg gctcgccgtt
     2641 cggcgcggat cgggatggtg gcgcccgcaa gcacgaaggc aacgacatcg cggcacaccg
     2701 gctcacgccc gtcgtggccg ttgcggacgg tgtgctgacc cgcatccgac aggagcgtgg
     2761 gacggacaat tgctgctggg cgatcgtcga gcacacggac ggctggca
//
LOCUS       DASTXO010000496         4771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1393067, whole genome shotgun sequence.
ACCESSION   DASTXO010000496 DASTXO010000000
VERSION     DASTXO010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393067"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VFU96_10520"
     CDS             <1..886
                     /locus_tag="VFU96_10520"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113741.1"
                     /translation="LGLETDFSLSTSLQARWFPANSTDYQWGWGSTDGNTGFLILLVV
                     RRYSLVTDSYTGHPALDVAVSHAALGAVARGEIEGLIRLHVPEPVVAFGVIDRVQPGY
                     PGAVRAAHANGFGAVERLAGGRAAVFHEQTLAFAWATPEDEPRAGINERFESVAGIMV
                     DAFESLGIDARIGQLRGEYCPGKWSVNVGGRVKVMGVGQRLVRGAAHVGGVVVVDDGE
                     RIRDVLIPVYRALQLDWDPRTTGALADRAPGLDNNKVSDAIIAAMSRRFELVPGEMPP
                     SVLEEANGLIADHLPRVA"
     gene            886..1974
                     /gene="aroQ"
                     /locus_tag="VFU96_10525"
     CDS             886..1974
                     /gene="aroQ"
                     /locus_tag="VFU96_10525"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEU5113742.1"
                     /translation="MRVLVLNGPNLNLLGTREPDVYGDTTLEDLESLIEGWGAELGIE
                     TRFTQSNHEGEVVDAIHEAAGNDGLIINPGALTHTSRAIGDAISSVGVPAVEVHISNI
                     KQREPWRAKSLVAPSCVRTIFGRGVGGYQDALRHLQNRAATPFETVAYGPDPDNVGDI
                     RRPDGEESGLVVLTHGGLWRQEYERDSTETLAVDLTHRGLVTWNIEYRRGSEGGWPGS
                     GHDVVSAIDFIVRKMPGVPLALIGHSAGGHLSLWAAARREDLIRLVVGLAPITDLAGL
                     ARSGSVGSREAQHLLDSGAPATLDPVAGKTLLVHGVDDEIVPVSHSTNLSTGSRVEVV
                     PGMGHFPMLDPKREHWPPIVAELGKALG"
     gene            2083..2670
                     /locus_tag="VFU96_10530"
     CDS             2083..2670
                     /locus_tag="VFU96_10530"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEU5113743.1"
                     /translation="MRLRRLSFIAGWAAVALLAVAGFAAFGTSGSTQAEPEFDATEPG
                     LGHSTGAQAGFNFAVLAGQGAVVDGAAATATTIATEEATAPADQSTGTIASDGSGWLS
                     QVQVRALVAEYFAAEDVNKAVRVAWCESRFNPASTNLRTGAVGLFQHLPKYWAERAES
                     AGFAGADPTDAEASTAAAAWAVYNEGGWDTFACRG"
     gene            2689..3081
                     /locus_tag="VFU96_10535"
     CDS             2689..3081
                     /locus_tag="VFU96_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018162894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HEU5113744.1"
                     /translation="MSGHNVLGGDLEPCGTEPLTGFYRDGDCATGFEDVGSHTVCAVV
                     TAEFLDHQRGIGNDLTTPMPEYRFPGLSPGDRWCVTAVNWLRAHYDGMAAPVVLASTH
                     EMALEIVPLEVLQKYAVDVPPDPSALES"
     gene            3140..4363
                     /locus_tag="VFU96_10540"
     CDS             3140..4363
                     /locus_tag="VFU96_10540"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU5113745.1"
                     /translation="MKRRLVALLVTSVVMLACAASPPERGSLPDVNEALDPPAQCAFL
                     VGEPQGVLVQEVSPDSAADGLLIVGDVIVAFDNDPTLDSESLLAALGEKDVGDVVELS
                     VRRGDEETHQTLTLGDASGEPRIGVMIRTQYEAVEATDVETEVEPGPLTRPISIAGLI
                     YLFDAGSNVWERTDVEVGEEVNWVATTASLYAIQDEIITDLVSGEEVAHDGFQAWEPI
                     RVIGSAGEDLILVVTQEVPDEPERVAVGVSRFNPFTSETLWAQPVVDGFGIPISAWGS
                     PDGETIAVVGVNEGGSEITGIDIWNGDGIPAGMEDLVPLGTPVGWMDAESLLFRTDVE
                     VASLLTVPGGDVEEIALEPAIGGLPLFPVGDGSGVLALDDQSLVLDDLTVAGDLNVLA
                     ENCSFDRVGEPGWSP"
     gene            complement(4360..>4771)
                     /locus_tag="VFU96_10545"
     CDS             complement(4360..>4771)
                     /locus_tag="VFU96_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113746.1"
                     /translation="EGSFGMGKGLASAMNTRESGMGGQASPVSIALREQRAEIEASLE
                     MLYERVNELLEANQDKILELAAVLEEKKTISGDEVAEIMESAPGSQTMREPTGWQAVS
                     DEVAGERQRVALERSGREVASTSNNPQTIDGEES"
BASE COUNT          804 a         1512 c         1581 g          874 t
ORIGIN      
        1 actcggcttg gagaccgact tctccttgtc aacgagcctc caggctcgct ggttccccgc
       61 aaattcgacg gattaccaat gggggtgggg atccactgac ggtaacaccg ggttccttat
      121 cctcctggtt gtgcgccgat acagcctcgt caccgattcg tataccggtc acccggcact
      181 cgacgtcgca gtgagtcacg ccgccctcgg agctgtggcc cgcggcgaaa tcgaaggtct
      241 cattcgtctt cacgtccccg agcccgttgt tgccttcggg gtgatcgatc gtgtccagcc
      301 cggctatcca ggggcggtcc gcgccgccca cgccaacggc ttcggagcgg tcgaacgctt
      361 ggccggagga agggccgcag tgtttcacga gcaaacactc gccttcgcgt gggccacgcc
      421 agaggatgag ccgagagccg gaatcaacga acggttcgaa agcgtggccg gaatcatggt
      481 ggatgcgttc gagagtctcg ggatagatgc gcggatcgga cagctccgtg gcgagtactg
      541 cccggggaag tggtcggtga atgtgggggg tcgggtcaag gtgatgggag tcggccagcg
      601 cctggttcgc ggggcggctc atgtgggagg cgtggtcgtg gtcgacgacg gagagcgaat
      661 ccgagacgtg ctcattcctg tctaccgggc actccaactc gattgggacc cgcgcaccac
      721 aggagccctc gccgatcgtg cccccggact cgacaacaac aaggtgtccg atgccatcat
      781 cgccgccatg tccaggcggt tcgagctcgt ccccggcgag atgcccccct cggtgctgga
      841 agaggcgaac gggctgattg ccgatcatct cccccgagtg gcgtgatgcg cgtcctcgtg
      901 ctcaatgggc caaatctgaa tctgctcggc acccgtgagc ccgacgtcta tggcgacacc
      961 actctggagg acctcgaatc cctcatcgag ggctggggtg cggagttggg catcgagacg
     1021 cgattcaccc agtcgaatca cgaaggggag gtcgtcgatg ccatccacga ggctgccggc
     1081 aacgatgggc tgatcatcaa ccccggtgcg ctgacccaca cctcccgggc gatcggcgac
     1141 gccatctcat cggttggtgt gcccgcggtg gaggtgcaca tctccaacat caagcagcgg
     1201 gagccctggc gcgccaagtc gctggtggcg ccgagctgtg ttcgaaccat tttcggccgt
     1261 ggtgtaggcg gttatcagga tgcgctgcga catctgcaga accgggcggc gaccccgttc
     1321 gaaacggtcg cctacgggcc ggatccagac aatgtggggg acatccgccg acctgacggt
     1381 gaggagtcgg gactagtggt gttgacccac ggcgggctct ggcgacagga gtacgagaga
     1441 gactccaccg agacgctggc cgtggatctg acccatcgag ggcttgtcac ctggaacatc
     1501 gaataccgga gaggcagcga aggaggctgg cccgggtccg gtcacgatgt cgtctccgca
     1561 atcgacttca tcgtcagaaa gatgcccggt gtgccgctcg ccctgatcgg acactccgcc
     1621 ggtggacacc tcagcctctg ggcggcggcc cgaagggagg acctcatccg actggtagtt
     1681 ggtttggcgc cgatcacgga cctggccggc ttggcgaggt caggctctgt cggatccagg
     1741 gaggcccagc acctgctgga ctcgggcgcc ccggcgacgc tggaccccgt cgccggcaag
     1801 accctcctcg tgcacggggt tgacgacgaa atcgtcccgg tgtcccactc gacgaatctg
     1861 tccacaggca gccgcgtcga ggtggtgccg ggaatgggtc atttccccat gctcgacccc
     1921 aagagggaac attggccacc aatagtggcc gagttgggga aagctctcgg ctgagagaaa
     1981 ccgagttgcc tcagttatcc acagatcggt agggtctatc tcggactgtg gtcttcctgc
     2041 gcaccgcgcg gggtctcgag tccacagtgg agtcaacttc atatgcgttt gagacgcctc
     2101 tcgtttatcg ctggatgggc ggcagtcgca ctcctggcgg tagccggctt tgcggcgttt
     2161 ggtacttcgg gcagtactca ggccgagccc gaattcgacg cgaccgaacc gggtcttggt
     2221 cacagcacag gggctcaggc aggattcaac ttcgcagtgc tcgccggcca gggcgcggtc
     2281 gttgacggcg cagccgccac tgcgaccacg atcgccacgg aggaggcaac agcccccgcc
     2341 gaccagtcga ccgggactat tgcctcggac gggtccggat ggctctccca ggtccaggtt
     2401 cgggctctcg ttgccgagta tttcgccgca gaggatgtga acaaggcagt ccgagtcgcc
     2461 tggtgcgagt cccgcttcaa cccggcttcg accaatttgc gcaccggtgc cgtcggtctg
     2521 ttccaacatc ttcccaagta ctgggcggag agggcggaga gcgcgggatt cgccggcgcc
     2581 gatcccaccg atgccgaggc ctctaccgcc gccgcggcat gggcggtcta caacgaaggc
     2641 ggctgggaca cgttcgcctg ccgcggctga gctcactctc ggcctgaaat gagcggtcac
     2701 aacgttcttg gcggcgatct ggaaccctgt ggcaccgaac cgctcaccgg gttctaccgg
     2761 gacggtgatt gcgccaccgg gttcgaagac gtcggaagcc acacggtgtg cgcggtcgtc
     2821 accgccgagt tcctcgacca tcagcgcggg atcggcaacg acctgaccac gccgatgccc
     2881 gagtatcgct tcccagggct ctccccggga gacaggtggt gcgtcacggc ggtgaactgg
     2941 ctgagggcgc actacgacgg gatggcagcc ccggtcgtgc tggcttcgac ccacgagatg
     3001 gcactcgaga tcgtcccgct cgaggtgctc cagaagtacg cggtcgacgt cccacccgac
     3061 ccgagcgccc tcgagagctg agtcgcgggc ggcggtgctg tctccgagtg aagcctcggt
     3121 gaaccggtac gatttgggcg tgaaaaggcg gctggtcgcg ttgctcgtca cctctgtcgt
     3181 gatgctcgcc tgcgccgcct cgccgccgga gcgcggttcg cttcccgatg tcaacgaggc
     3241 gttggacccg ccggcgcagt gcgccttcct ggtgggcgag ccacaaggcg tcctggtgca
     3301 agaagtcagc cccgattcgg ccgctgacgg cctcctgatc gttggcgacg tcatcgtcgc
     3361 cttcgacaac gacccgacgc tggattccga gtcgctgctc gcggctctgg gagaaaagga
     3421 cgtcggggat gtcgtcgagc tctccgtccg gagaggcgac gaggagacac atcagacact
     3481 gaccctgggc gacgcctctg gcgagccgag gatcggagtg atgatcagga cccagtatga
     3541 ggctgtcgaa gcaaccgacg tcgagaccga ggtggagccg gggccactga cccgtcccat
     3601 ttcgatcgcc ggtttgatct acttgttcga cgcggggagc aacgtctggg agcgaaccga
     3661 tgtcgaggtg ggcgaagagg tcaactgggt cgccaccacc gcgagtctct acgcgatcca
     3721 ggacgagatc atcaccgacc tggtctcagg cgaggaagtc gcccacgacg ggttccaggc
     3781 gtgggagccg atccgggtga tcgggtcggc gggcgaagat ttgatcctcg tcgtcactca
     3841 ggaggtgccc gacgagccgg agcgggtggc ggtgggtgtc tcgaggttca acccgttcac
     3901 gtcggagact ctctgggccc agccggtcgt cgacgggttc ggcattccca tctccgcatg
     3961 ggggtcgccc gacggcgaga ccattgctgt cgtcggcgtg aacgaaggtg gctcagagat
     4021 tacgggtatc gacatctgga atggtgacgg catccccgcc gggatggagg acctggtccc
     4081 gctcgggact ccggtcggtt ggatggacgc cgaatccctg ttgttcagga cggacgtcga
     4141 ggtcgcatcg ttgctcacgg ttcctggcgg cgacgtcgag gagatcgccc tggaacccgc
     4201 catcggcggt ctccctctgt tcccggtcgg tgacgggagc ggcgtcctcg cactcgacga
     4261 ccaatcactc gttctcgatg acctgaccgt ggccggagac ctcaatgtcc tggccgagaa
     4321 ctgcagcttc gatcgggtcg gagagcctgg ctggagccct tagctctcct ccccgtcgat
     4381 tgtctgcggg ttgttgctgg tggacgccac ctctcgtccg gagcgctcca gtgcgacacg
     4441 ctggcgctct ccggccactt cgtctgaaac tgcctgccat ccggtcggct cacgcatcgt
     4501 ctgagagccg ggcgcgctct ccatgatctc agccacctcg tcgccagaga tcgtcttctt
     4561 ctcctcgagg acggccgcca gttcgagaat cttgtcctga ttcgcttcga gcagctcatt
     4621 cacccgctca tatagcattt cgagactggc ctcgatctcg gctcgctgct cgcggagtgc
     4681 gatggaaacc gggctcgcct gcccacccat ccccgattcc cgggtgttca tcgcggaagc
     4741 gagtcccttg cccatgccga aggacccttc c
//
LOCUS       DASTXO010000497         2357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_840696, whole genome shotgun sequence.
ACCESSION   DASTXO010000497 DASTXO010000000
VERSION     DASTXO010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840696"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VFU96_10550"
     CDS             <1..597
                     /locus_tag="VFU96_10550"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEU5113747.1"
                     /translation="ADRWRRAVEVLGTLRNDLLRPSDHDDLSFLWVIDFPLFEEADDG
                     SITFSHHPFTSPASLDEMRDNPTGAVSKAYDAVLNGVELGSGSVRIHDPDVQRQVFDI
                     LGIDADTAERRFGWFLEALRYGTPPHAGFAFGIDRLVMVLQGETSIREVIPFPKTQSG
                     VDPMTGAPTWVEPGQLDELGIALTEEALARREQPIDEG"
     gene            682..837
                     /locus_tag="VFU96_10555"
     CDS             682..837
                     /locus_tag="VFU96_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113748.1"
                     /translation="MTLITSSPTRIGRPPSIVAIIALLLFLGATSLAGGTAMVFGLGG
                     GEIMLPD"
     gene            874..1188
                     /locus_tag="VFU96_10560"
     CDS             874..1188
                     /locus_tag="VFU96_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113749.1"
                     /translation="MPGVVLGIGFGVGSLLTAYGVIRRPLWGWTSFLEQRTRQHWSWI
                     ATVALGVGHVVWIFLELLFLPEMSWFHPLYGAVGLGLARHSVSAFGEGAPSGALAVPS
                     TG"
     gene            1185..>2357
                     /locus_tag="VFU96_10565"
     CDS             1185..>2357
                     /locus_tag="VFU96_10565"
                     /inference="COORDINATES: protein motif:HMM:NF017321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEU5113750.1"
                     /translation="MSDLFSARADELTAQTAPLATRMRPRSLDEVVGQPALMAPDAAF
                     RRLVESGHPVSMLLWGPPGTGKTTLARLVASRAEAAFETLSATSAGVKDIREVLAGAK
                     SRLATDERRTVLFLDEIHRFAKNQQDALLPGVEDGTLILIGATTENPFFEVNSPLMSR
                     MTLFRLESLRPEELADLIKRAISDNDRGLGEMGLGIDPEAIEELGNRTGGDGRQALNS
                     LEVAALLATGDKRSVITIGDVAEALQRRIVRYDKVGDQHYDVTSAFIKSVRGSDPDAA
                     LYWLFLMLEGGEDPEFIARRLIILASEDIGLADPHALPLAVAAADALAYVGLPEAAYH
                     LTEATIYLAAAPKSNSVGRAMAKTRQLVTDGPAASVPPHLRSAAYPGAEKLGHGSGY"
BASE COUNT          396 a          761 c          750 g          450 t
ORIGIN      
        1 gccgatcgct ggaggcgggc tgttgaggtc ctgggaacac tacgaaacga cctgctccgg
       61 ccgtcagatc acgacgacct gtccttcctc tgggtgatcg acttcccact gttcgaagag
      121 gctgatgacg gcagcatcac cttctcccac catccattca catcgccggc ctcactcgat
      181 gagatgcggg acaacccgac cggggcggtc tccaaggcat acgacgcggt gctcaacggt
      241 gtcgagctgg gctcgggctc ggttcggatc catgatccag acgtgcagcg gcaggtgttc
      301 gacatcctcg gcatcgacgc cgacaccgcc gagcgtcgat tcggctggtt cctcgaagcc
      361 ctgcggtacg ggaccccacc ccatgccggc ttcgccttcg gcatcgaccg cctggtgatg
      421 gtcctccagg gtgagacctc gatccgagag gtaatcccgt tcccgaagac ccagagtggc
      481 gtcgatccga tgaccggcgc ccccacctgg gtcgagccgg gacagctcga cgaactgggc
      541 atcgccttga ccgaagaggc gctggcccgc cgggaacagc cgatcgacga aggctgacag
      601 atccccgaat tcatagtgac cttgacccct ggacggtcgc gctgcgcacc atgctcagtg
      661 gagtggaaag gaggcgtttc cgtgacgctc atcacatcga gccccaccag gatcggtcgg
      721 ccgccctcca tcgtcgcgat cattgctctg ttgctgttcc tcggagccac ctccctggcg
      781 ggcgggaccg ccatggtgtt cgggttgggg ggtggggaga tcatgttgcc cgactaatgg
      841 ctggagacaa ttccgatcat cgacagctgg ttggtgcccg gtgttgtcct cggcatcggg
      901 ttcggggtcg gctccttgtt gactgcctat ggagtgatcc gacgcccgct ttggggatgg
      961 acctcgttcc tggaacaacg cacacgccag cattggtcgt ggatcgcgac cgtcgccctc
     1021 ggtgtcggcc acgtggtgtg gatcttcttg gagctgctct tcttgcctga gatgtcctgg
     1081 ttccatccgc tctacggagc ggtaggcctt ggccttgctc gtcattccgt ttctgcattc
     1141 ggcgagggag caccttcggg tgctctagcg gtaccgtcaa cggggtgagt gacctcttct
     1201 cggctcgggc cgacgagctg acggcccaga ctgcccccct cgctacccgg atgcgcccgc
     1261 gatcgctgga cgaggtagta ggccagccgg ccttgatggc ccccgacgcc gcatttcgac
     1321 gtctcgtcga gtcgggtcac ccggtgtcga tgttgctctg gggcccaccg gggacaggca
     1381 agacgacctt ggcccgtttg gtcgcgtccc gggcagaggc cgccttcgag accctctccg
     1441 cgacgtctgc cggtgtgaag gacattcgtg aggtattggc cggtgccaag agccgcttgg
     1501 ccaccgatga gcgccgaacc gttctcttcc tggacgagat ccaccgattt gccaagaacc
     1561 agcaggacgc cctgcttccg ggtgtcgagg acggcacact gatcctgatc ggggcgacga
     1621 ccgagaaccc cttcttcgag gtcaattcac ctctcatgag ccggatgacg ctcttccgcc
     1681 tcgagtcact gcggcccgag gaattggcgg acctcatcaa gcgagccatc agcgacaacg
     1741 accgtggcct cggtgaaatg gggctgggga tcgacccgga agcgatcgag gagttgggca
     1801 accggaccgg tggggatggg agacaggcgc tcaactcgct ggaagtggct gccctcctcg
     1861 ctaccggcga caaacgatcg gtgataacca tcggagacgt cgccgaggcg ctgcaacgac
     1921 gcatcgtgcg ctatgacaag gtgggggacc agcattacga cgtcacctcg gcctttatca
     1981 agagcgtccg aggctccgat ccagatgctg ctctctattg gctgttcctc atgttggagg
     2041 gcggagagga tcccgagttc atcgcccgaa gactcatcat cctcgcgtca gaagacatcg
     2101 gcttggccga tcctcacgcc ctgccattgg cagtggcggc agccgacgcc ctcgcctatg
     2161 tcgggctgcc ggaggcggcc tatcacctga ccgaggcgac gatctatctg gccgccgccc
     2221 ccaaatcgaa ttcagtgggc cgggccatgg cgaagacgcg tcagctcgtc accgacggcc
     2281 cggctgcgag tgttccccca catctgcgct cggcggccta cccgggggcg gagaagctgg
     2341 gccatggttc gggctac
//
LOCUS       DASTXO010000498         2145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1246107, whole genome shotgun sequence.
ACCESSION   DASTXO010000498 DASTXO010000000
VERSION     DASTXO010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246107"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1261
                     /locus_tag="VFU96_10570"
     CDS             <1..1261
                     /locus_tag="VFU96_10570"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5113751.1"
                     /translation="NDVLAPVLAFDTDIHAAKAEGIWITDVDGKRWADFACGTAVTNL
                     GHNHPAVLEAAHAQLDNLVHSGCIVRYDSIVEAAERLRAITPPGIEKFSFANSGAEAV
                     EAAVKLAKHTTGRQGVVVFRGAFHGRTMGSVTYTTSNARYRDGYHPVLGSVFVTPYPH
                     PYRWGMTEAEATDLSLNELQMMFKHVVTPRNIACFLIEPVQGEGGYYPAPARFLDALR
                     EMADEHGIKLLYDEVQSGFGRTAEWFATDHYDAKPDIMAMGKGIANGLPLSAFGSSRQ
                     TIDAWPRGAHGTTYGGNPVSTAASVAVIDTMEGLLPHARELSKRAFERFNEMADQHPT
                     IGDVRGLGLMIGVELVTDRESRTPDATAFNHVEKHAFDRELIIINCGPDANVIRFIPP
                     LVTTSEELDWALDLVDEGLSSWESTDR"
     gene            1258..2073
                     /locus_tag="VFU96_10575"
     CDS             1258..2073
                     /locus_tag="VFU96_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015746155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEU5113752.1"
                     /translation="MTIRRLLFAVAALVLGVVLFVMLRLEAGPAVTTVAAAPTTTMTT
                     SSTSSTSPTSSTTITTTPSTTTTTTAATTTQTAATTTLASPVFQSSIGMVGAADLPAS
                     WREGCPISVEALRSVDVSHFGYDGEIHTGRLIVSAEVAEDMVEIMSQLFVAAFPIERM
                     EPVDVYEGDDDLSMAANNTSAFNCRAVTGGSSWSEHSYGTAIDVNPLVNPYVVGGTVL
                     PPEGAAYADRSLDAPGMIHDGDVVVEAFASHGWIWGGTWSSPTDYQHFSTSGR"
BASE COUNT          395 a          727 c          655 g          368 t
ORIGIN      
        1 caacgacgtt ctcgcccccg tgctcgcctt cgacacggat atccacgcgg ccaaggccga
       61 ggggatctgg atcaccgacg tcgacggcaa acgctgggcg gacttcgcct gtggaaccgc
      121 ggtgaccaac ctggggcaca atcacccggc cgtgctggaa gcagcccacg ctcagctcga
      181 caacctggtg cactctggat gcatcgtccg gtacgactcc atcgtcgagg ctgccgaaag
      241 attgcgggcg atcaccccgc cggggatcga gaagttcagc ttcgccaatt ccggtgccga
      301 agcggtcgag gcggcggtga agctggccaa gcacaccacc ggccgacaag gtgtggtcgt
      361 tttccgaggg gcattccacg gccggaccat ggggtcggtc acttacacca cctcgaacgc
      421 caggtatcgc gacgggtatc accccgtcct cggctcggtc ttcgtcacgc cgtatccgca
      481 cccgtatcga tggggtatga ccgaggccga ggccaccgac ctctccctca acgaattgca
      541 gatgatgttc aagcacgtgg tgacgccgag gaacatcgcc tgctttctca tcgagccggt
      601 gcagggtgag ggtggctact accccgcccc ggcccggttc ctcgatgccc ttcgcgagat
      661 ggccgatgag cacgggatca agctcctcta cgacgaggtc cagtcgggct tcgggcgcac
      721 cgccgagtgg ttcgccaccg accattacga cgccaagccc gacatcatgg ccatgggcaa
      781 agggatcgca aacggtctcc ccctgtcggc attcgggtca tcaaggcaaa cgatcgatgc
      841 gtggcccagg ggcgcgcacg gcaccaccta tgggggaaat ccggtctcga ctgcggcctc
      901 ggtggccgtg atcgacacga tggaagggct gctcccccac gcccgggagc tgtccaagcg
      961 cgccttcgag cgattcaacg agatggccga ccaacacccg acgatcggag acgtgcgcgg
     1021 cctcggcctc atgatcggag tcgagctggt gaccgatcgg gagagtcgca cgccggacgc
     1081 gaccgccttt aatcatgtcg agaaacacgc cttcgaccgt gagctgatca tcatcaactg
     1141 cggcccggac gccaacgtca ttcgcttcat cccgccgctc gtcacgacga gcgaagagct
     1201 cgactgggcc ctcgacctcg tggatgaggg gttgagcagc tgggagagca cggaccgttg
     1261 accatcaggc ggctgctctt cgcagtagcc gcgctcgtct tgggtgtcgt gctctttgtc
     1321 atgcttcggc tcgaggccgg gccggctgtc accacggtgg ccgccgcccc cacgacgacc
     1381 atgacgacat cctccacatc ctccacatcc cccacgtcct cgaccaccat caccactaca
     1441 ccatctacca ccaccaccac cacggccgcg accacgactc aaaccgccgc cacgacgact
     1501 ctcgcctcgc cggtgttcca atcttccatc ggcatggtcg gggccgccga cctccccgcc
     1561 tcgtggcggg agggctgccc aatctccgtc gaggctttga ggtcggtgga cgtgtcccac
     1621 ttcggatacg acggggagat ccacacggga cggctgatcg tttccgcaga ggtcgcagag
     1681 gacatggtcg agatcatgtc gcagctgttc gtggccgcgt tcccgataga gcgaatggaa
     1741 cccgtcgacg tctacgaggg cgacgacgac ctgagcatgg cggcgaacaa cacaagcgca
     1801 ttcaattgcc gagcagttac cggcggatcg tcgtggtcgg agcactcgta cgggaccgca
     1861 atcgacgtga accccctggt caacccatat gtcgttggcg ggaccgtgct accaccggag
     1921 ggcgcggcct acgccgatcg aagtctcgac gcaccaggaa tgatccacga tggcgacgtc
     1981 gtcgtggagg ccttcgccag ccacgggtgg atctggggtg ggacctggtc ctccccaacg
     2041 gactaccagc atttctcgac ttcgggtcgc tgagggtctc tacgcggcgc cggtgaagaa
     2101 ctccagcgcc gtcttggtct tggtctgctg ttccccagtg agctc
//
LOCUS       DASTXO010000499         7021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_620156, whole genome shotgun sequence.
ACCESSION   DASTXO010000499 DASTXO010000000
VERSION     DASTXO010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7021
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620156"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VFU96_10580"
     CDS             complement(<1..827)
                     /locus_tag="VFU96_10580"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HEU5113753.1"
                     /translation="MVDLRNDSITIGKVTVPPGRKMRIELPFAHLPAGTEASLPIAVI
                     NGRTSGPNVWVSGGIHGDEINGVEVVRKVMRSLDAKRLRGAVIAVPIVNPLGFMVRSR
                     YVPDRRDLNRVFPGSPRGSTASRLAHLFMNEVVSHCSVGIDCHTASNHRTNLPQLRAD
                     IDDPDTLDLARAFGAPFTIKARVRDGSLRQAATEQGIKMLLYEAGQADRTDEDAIAIG
                     FEGVMRTLRSMGMIEMRLPRPRPTTLVRRTRWMRARRGGLVEISPKLGDRVEEGDEVA
                     "
     gene            complement(820..1719)
                     /gene="rimK"
                     /locus_tag="VFU96_10585"
     CDS             complement(820..1719)
                     /gene="rimK"
                     /locus_tag="VFU96_10585"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HEU5113754.1"
                     /translation="MKLAILSRNSGLYSTQRLREAALARGHEVAIVDYLRCYMEISAR
                     RPKILFRGEEVRPEAVIPRIGATYTFYGAAVVLQFEMAGVFTLNDSQAISRSRDKLRS
                     LQLLSRAGVGLPTTSFAHSTQDIDGLLEVVGGPPVVVKLLEGTQGLGVVLAETRKAAD
                     SVISAFRQLDANILVQEFVKEAGGADIRAFVVGGKVVAAMKRQGPPGEFRSNLHRGGH
                     AESIKLSPAERATAVRAAKTMGLNVAGVDLLQSDAGPMVLEVNSSPGLEGIEAASGID
                     IADAIIEFIEDHSDHGPARIDRG"
     gene            complement(1716..2285)
                     /locus_tag="VFU96_10590"
     CDS             complement(1716..2285)
                     /locus_tag="VFU96_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008309832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HEU5113755.1"
                     /translation="MPDRSRFSDLYDDDGDPGAVPIPISTTAVDYRGGMSRRADEHKA
                     IIGWREWVQLPDLGVVEMKVKVDTGADNSSLHAFNLERFERDGTEYVRFEIHPKQRSR
                     KPSIQCEAPLAMEKKVKNPGGRSELRPVIRTRVLVAGVELNALVNLTSRDEMGFRMLL
                     GRRAVRAKFVIDPGRSYLGARPGRPQDAE"
     gene            complement(2490..4172)
                     /locus_tag="VFU96_10595"
     CDS             complement(2490..4172)
                     /locus_tag="VFU96_10595"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEU5113756.1"
                     /translation="MAKHAAPLSIFSWAFLALGLILFALAGVGWSQHVSSEGVFVANN
                     VSYADEPMEGVTPEGVDTAVADRSTEILGRELMIDYGQGELAVPLGAVGFKYDQPATA
                     SAVLSARHEGSVWRQFSSWAISGVATDEIEEQWTFDPELARKALDNHPGLTPVVVTEP
                     RVEPDGGGRVVLIPGVIGTEADIDALVERLSEVDLIDPPDRLEIDVEEIHPTVSDETA
                     QATVDRLNSLTGDGVDISVDGKTARLSPAVLQSHLRFTVGGGEVGVTFDSSALHATLE
                     NTIVGPVGNFVEPTFDIIDDIPTVRAPGEPPPVCCEAGVGEWLGEQILAGSSGPFGLQ
                     SRPTVDPALAAWADGSAVVEKVSEFTTPHNCCETRVRNIQRIADIVRGVYLVPGETLS
                     LNEYVGPRTVENGFFAAGAIRQGHLIEEVGGGVSQFATTIFNAAYFAGMDFVEYRSHS
                     LYFSRYPYGREATISSPAPDLVMTNTTNYPILIWTSYTDESITVSMYSTKNVDIAELD
                     QRTSQRGACTHVETDRQRTYADGRVVVDTFLADYRPAEGIDCSGNPIPPPPG"
     gene            4436..>7021
                     /gene="rpoB"
                     /locus_tag="VFU96_10600"
     CDS             4436..>7021
                     /gene="rpoB"
                     /locus_tag="VFU96_10600"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018638481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU5113757.1"
                     /translation="MVSRVAERFSFGKIPEVLPLPDLLAVQHESFKWFLDEGFREIFE
                     EISPIEDFTGSLALELTDHRFGEPQMSLEEAKERDANYAMPLFVTARFMNRQTGEIKE
                     QQVFLGDFPIMTDKGVFIINGTERVIVSQLVRSPGIYFDKTPDKTSDREIYSGKIIPG
                     RGAWLEFDTDKKDTIGVRVDRKRRQYITAFLKALGIAETDEEILAMFDGAESIKNTIE
                     KDTAHSKEEALLDLYRKLRPGEPTTVESARGLIQTLFRNPKRYDLTRVGRYKISQKYR
                     EDTPADYRAEGLQLLRDEDIIDAIKYLVALHAGQTTMTTHTGTEVAVNTDDIDHFGNR
                     RIRTVGELVQNQVRVGLTRLERVVRERMTTQDPDAITPQSLINIRPVTAAIKEFFGTS
                     QLSQFMDQPNPLAGLTHRRRLSALGPGGLSRERAGFEVRDVHPSHYGRMCPIETPEGP
                     NIGLIGSLASYARVNEFGFIETPYRKVESGKVTTKVEYLTAIEEERFIIAQANAPLKE
                     DGAFQNPRVLVRRAGGEVEYVPPKDVDYMDVSPKQIVSVSTSLIPFLEHDDANRALMG
                     SNMQRQAVPLLTSDAPLVGTGVEQRAAQDSGDMIVSPIVGEVVEVTGDEIVIKEKETN
                     KKHTFVLDKFRRSNQGTCMNHKPLVREGDTVKVGDVLADGPSTETGELALGRNLLVAF
                     MPWQGHNFEDAIIISERLVKDDVLTSIHIEEHEIDARDTKLGAEEITRDIPNVPEEVL
                     ANLDEEGIIRVGAEVGPGDYLVGKVTPKGETELTPEERLLRAIFGEKAREVKDTSLRV
                     PHGVHGKVIDVRSFRRDDDSDHELPAGVNEMVRVSIALKRKISEGDKMAGRHGNKGVI
                     SRILPI"
BASE COUNT         1306 a         2278 c         2179 g         1258 t
ORIGIN      
        1 gcgacctcat cgccttcctc gacgcggtct cccagcttcg gcgagatctc gaccagaccg
       61 ccacgccgcg ctctcatcca tcgagtccgt cgcaccagag tggtgggacg cggcctcggc
      121 aacctcattt cgatcatgcc catcgagcga agcgtccgca tcactccttc gaagccgatt
      181 gcgatcgcat cctcatcggt tcggtccgcc tgacccgcct catagaggag catcttgatg
      241 ccctgttccg tcgcggcctg ccgaagcgag ccgtctcgga cacgggcctt gatggtgaaa
      301 ggggcaccga aggcccgagc tagatcgaga gtgtccgggt cgtcgatgtc ggcccggagt
      361 tgggggagat tggtccggtg gttcgacgcg gtgtggcagt cgatcccgac cgagcagtga
      421 gagaccacct cattcatgaa aaggtgggcg agacgcgatg ccgtcgaccc gcggggtgag
      481 ccagggaaga ctcggttcag atcgcggcga tcggggacgt acctggagcg gaccatgaaa
      541 ccgagcgggt tcacgatcgg caccgcgatc accgcccctc gcaacctctt ggcatcgagt
      601 gacctcatca ccttgcgcac cacctcgacg ccgtttatct catctccgtg gatgcctccc
      661 gatacccaga cgttggggcc ggaggtgcga ccgttgatca ccgcgatggg gagcgaggcc
      721 tcggtgcccg ccgggagatg ggcaaatgga agctcgatcc gcatcttgcg tcccggcggc
      781 acggtgacct tgccgatcgt tatcgaatcg ttgcggaggt caaccacggt cgattcgggc
      841 cggcccgtgg tcggagtggt cctcgatgaa ctcgatgatg gcgtctgcga tgtcgatccc
      901 gctcgccgcc tcgatccctt ccaagcctgg tgacgagttg acctcgagca ccatcggacc
      961 ggcatccgac tggagaaggt cgactccggc gacgttcaat cccatcgtct tcgccgcacg
     1021 caccgcggtc gctcgttccg ctggcgacaa tttgatcgac tcggcgtgcc cgccgcgatg
     1081 gaggttcgat ctgaactcac cgggcggacc ctgtcgcttc atcgcggcga ccaccttccc
     1141 gccgaccacg aacgccctga tgtcggcccc cccggcctcc ttgacgaact cctggaccag
     1201 gatgttggca tcgagctggc ggaaggccga gatcacggag tcggccgctt ttcgcgtctc
     1261 tgcgaggacc acgccaagcc cttgagtgcc ctcgagcagc ttgaccacga ctggggggcc
     1321 acccaccacc tcgagcaagc catcgatgtc ctgggtggaa tgggcgaagc tggtcgtggg
     1381 gagcccgaca ccggcgcgtg acaggagctg caacgaccgc agcttgtccc gtgaccggct
     1441 gatcgcctgc gaatcgttaa gcgtgaagac gcctgccatc tcgaactgga ggaccactgc
     1501 tgccccgtag aacgtatagg tggcaccgat gcgcgggatc accgcctcag gcctcacctc
     1561 ctccccgcga aacaggatct tcggccttct cgccgatatc tccatgtagc agcggagata
     1621 gtcgacgatt gccacctcgt ggcctcgggc caaagcggcc tcccggagcc gctgggtcga
     1681 atagagacca gagttgcggg agaggatggc gagtttcatt ccgcatcctg cggccgaccg
     1741 ggccgagccc cgaggtagct gcggccggga tcgatgacga atttggctcg caccgctctt
     1801 cggccgagga gcatgcgaaa tcccatctcg tcccggctgg tcaggttgac cagtgcgttc
     1861 agctccactc cggccacgag cactctggtg cggatgacgg ggcgcagctc acttcgacca
     1921 cccgggttct tcaccttctt ctccattgcc aggggagcct cgcattggat ggaaggcttc
     1981 ctcgatctct gcttgggatg aatctcgaag cgaacgtact cggtgccgtc ccgttcgaag
     2041 cgctcgaggt tgaaagcgtg gagcgaggag ttgtcggcac ccgtgtcgac cttcaccttc
     2101 atctcgacca cgcccagatc aggcagctgg acccattccc gccaaccgat gatcgccttg
     2161 tgctcgtcgg cccgtcggct catgccgccc cggtagtcga cggccgtcgt ggaaatcggg
     2221 atcggcaccg cacctggatc accgtcgtca tcgtagaggt cggagaaccg ggaacggtcg
     2281 ggcacaatga ttcgtgatga agaccgggcg acgtgagaag ccgaggaaga agatcccggc
     2341 ctggctgatg ggcttcatct tcgcggtgat catctccgtc gtggccttgc tcatcctcga
     2401 tgccatgggc tacggggacg acccctccgt gggcgcactt ggaatggttg cggtggccct
     2461 gcgtagagcc ggccgtcaac cgtgcccggc tatcccggag gtggcgggat cgggtttccg
     2521 ctgcagtcga tgccctctgc cggccggtag tcggcgagga atgtgtcgac gactacccgt
     2581 ccatcggcgt aggtccgttg ccggtcggtc tccacgtggg tacatgcccc gcgttgggat
     2641 gtccgttggt cgagctccgc gatgtccacg ttcttcgtgg agtacatgct gacggtgatt
     2701 gactcgtccg tgtaggaagt ccagatcagg atcgggtaat tggtggtgtt ggtcatcacc
     2761 aggtccgggg cgggtgatga gatggtggcc tcacgcccgt agggataccg tgagaagtag
     2821 aggctgtggc tccggtactc gacgaagtcc atcccagcga agtaggcggc gttgaagatc
     2881 gtggtggcga actgggacac cccgcctccg acctcctcga tcagatgacc ctggcgtatc
     2941 gcacccgcgg cgaagaatcc gttctcgacc gtgcggggac cgacgtattc gttgagcgag
     3001 agtgtctcac ccggcaccag gtagacaccg cgcactatgt cggcgattcg ctgaatgttc
     3061 ctcactcgcg tctcacaaca gttgtgtggg gtggtgaatt cgctcacctt ctcgacgacg
     3121 gcgctgccat cggcccaggc ggccaaggcg gggtcgactg ttggccgcga ctgcagcccg
     3181 aagggaccgc tcgatccagc caggatctgc tcgcccagcc actcacccac ccctgcttcg
     3241 caacagacgg ggggcggttc gccgggagct cggacggtcg ggatgtcgtc gatgatgtcg
     3301 aaggtgggct cgacgaaatt gcccacaggc ccgacgatcg tgttctcgag tgtggcgtgc
     3361 aatgcagagc tgtcgaatgt gaccccgacc tcgccaccac caaccgtgaa acggagatgt
     3421 gattgcagca ccgcggggct cagcctcgcg gtcttcccgt cgaccgagat gtcgacaccg
     3481 tccccggtca aggaattcaa gcggtcgacc gtcgcctggg cagtctcgtc ggacaccgtt
     3541 gggtgaatct cttccacgtc gatctcgagc cggtcgggcg ggtcgatgag gtctacctcg
     3601 ctgagtcgtt cgaccagcgc gtcgatatcg gcctcggtgc cgatcacccc gggaatcagc
     3661 accactcgac cgccaccatc aggctcgacc ctcggctcgg tgaccacgac cggggtcagc
     3721 cccgggtgat tgtcgagtgc cttcctcgcc agctcgggat cgaaggtcca ctgctcctcg
     3781 atctcgtcgg ttgccacccc gctgatggcc cacgacgaga actgcctcca gaccgatccc
     3841 tcatgtcgag cggagagcac ggcggatgcc gtcgccggtt ggtcgtactt aaagccgacg
     3901 gcccccagcg ggaccgcgag ctcgccctgc ccgtagtcga tcatgagctc ccggccgagg
     3961 atctcagtgg agcgatccgc caccgccgtg tcgacgcctt caggcgtgac accctccatg
     4021 ggctcatccg cgtagctcac gttgttggcc acgaacacgc cctcactgga aacgtgttgt
     4081 gaccaaccca ccccggccag ggcgaacagg atcaagccga gcgccaggaa cgcccaagag
     4141 aagatggaga ggggagcagc atgcttggcc acggtgcccg gtcatggtac gagtcagccg
     4201 gatcttgtag gtggacctgg gagacgtctc atccataccc ttgacggctt tctcggcgcc
     4261 gctgtatgct gaccaccgac cttcgggtcc gggacggatc ggatctttcg tcgccaagac
     4321 ggagatgcga ccgtttcgac aactgaagat ttcaattgga ggcatgctga cgcgtctgtt
     4381 agcatgcctt gtctgcgtta cttagacctg atccggatcc ctcaggaggc cccccttggt
     4441 ttcgcgcgtt gctgagcgct tctcatttgg aaaaatcccc gaagtactac ctctccccga
     4501 cctcctcgca gtccaacacg aatccttcaa gtggttcctc gacgagggtt tccgcgagat
     4561 cttcgaagag atcagcccca tcgaggactt caccggctcc ctcgccctcg agctgaccga
     4621 ccatcggttt ggcgagccgc agatgagcct cgaggaggcc aaggagcgtg acgccaacta
     4681 cgcgatgccc ctattcgtca ccgcccggtt catgaaccgg cagacaggtg agatcaagga
     4741 gcagcaggtc ttcctgggtg acttcccgat catgaccgac aagggcgtgt tcatcatcaa
     4801 cgggaccgag cgcgtgatcg tctcccagtt ggtgcgctcc ccgggcatct acttcgacaa
     4861 gaccccggac aagacctcgg acagagagat ctactccggc aagatcatcc cgggccgcgg
     4921 cgcgtggctg gaattcgaca ccgacaagaa agacaccatc ggggtcaggg tcgaccgcaa
     4981 gcgccggcag tacatcactg ccttcctcaa ggcactcgga atcgccgaga ccgatgaaga
     5041 gatcctggcg atgttcgacg gcgccgagtc gatcaagaac accatcgaga aggacaccgc
     5101 ccattccaag gaagaggcac tgctcgatct ctatcgcaag ctgcgcccgg gcgagccgac
     5161 cacggtcgag tcggcccgcg gtctgatcca gactctgttc cgcaacccga agcgctatga
     5221 cctgaccagg gtcgggcggt acaagatcag tcagaagtat cgcgaggaca ctccggccga
     5281 ctaccgggcc gagggcctcc agctgttgcg tgacgaagac atcatcgacg ccatcaagta
     5341 tctggtggcg ctgcacgccg gccagacgac tatgacgacc cacaccggga ccgaggtcgc
     5401 ggtcaatacc gacgacatcg accatttcgg gaaccgccgc attcgtaccg tcggtgagct
     5461 ggtccagaac caggttcgcg tcgggctgac tcgtctcgag cgcgtcgtcc gcgagcggat
     5521 gaccacgcag gaccctgatg ccatcacgcc gcagagcctg atcaacatcc ggccggtcac
     5581 ggccgccatc aaggagttct tcgggacctc gcagctatcc cagttcatgg accagcccaa
     5641 cccgctggcc gggctcacac accgtcgtcg tctctccgcc ctcggccctg gtgggctttc
     5701 ccgtgagcgg gccgggttcg aggtcagaga cgtccatccc tcccactacg ggcggatgtg
     5761 cccgatcgag actcccgaag gccccaacat cggcctgatc ggctccctcg cctcgtacgc
     5821 ccgagtcaac gagttcggct tcatcgagac cccgtatcgc aaggtcgaat cgggcaaggt
     5881 caccacaaag gtggagtatc tgaccgcgat cgaggaggag cgattcatca tcgctcaggc
     5941 gaacgccccg ctcaaggagg atggcgcctt ccagaacccg agggtcctgg tacgtcgtgc
     6001 cggtggggag gtcgagtacg tgccgcccaa ggacgtcgac tacatggacg tctcgccgaa
     6061 gcagatcgtg tcggtttcca cctcactcat ccccttcctc gagcacgacg acgccaaccg
     6121 ggccctgatg ggctccaaca tgcagcgcca ggctgtgcca ctcctcactt cggacgcccc
     6181 gctggtcggg accggggtcg agcagcgcgc agcacaggac tccggcgaca tgatcgtgtc
     6241 cccgatcgtc ggtgaggtgg tcgaggtcac cggtgacgag atcgtcatca aagagaagga
     6301 gaccaacaag aagcacactt tcgtgctcga caagttccgc cgctccaacc agggcacctg
     6361 catgaaccac aagccgctgg ttcgggaggg tgacaccgtc aaggtgggcg atgtgctcgc
     6421 cgacggtccc tccacggaga ccggtgagct cgcgctcggg agaaacttgc tggttgcctt
     6481 tatgccatgg cagggccaca acttcgaaga cgccatcatc atctccgagc gtttggtgaa
     6541 ggacgacgtg ctcacctcga tccacatcga ggagcacgag atcgacgccc gtgacaccaa
     6601 gctcggcgca gaggagatca ccagagacat ccccaacgtc cccgaagagg ttctggccaa
     6661 cctcgacgag gaggggatca tccgggtcgg cgccgaagta gggcccggcg attacctcgt
     6721 cggcaaggtc accccgaagg gcgagaccga gctgaccccg gaggagcggc tgctgcgggc
     6781 gatcttcggc gagaaggcgc gcgaggtgaa ggacacctcg ctgcgggtgc cccacggcgt
     6841 ccacggcaag gtcatcgacg tgcggtcgtt ccgccgtgat gacgattcag accatgagct
     6901 gccagccggc gtgaacgaga tggtgcgcgt cagcatcgcc ctaaagcgga agatctccga
     6961 gggcgacaag atggctggtc gccacgggaa caagggcgtc atctcgcgca ttctgccaat
     7021 c
//
LOCUS       DASTXO010000500         3957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1430284, whole genome shotgun sequence.
ACCESSION   DASTXO010000500 DASTXO010000000
VERSION     DASTXO010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430284"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFU96_10605"
     CDS             <1..204
                     /locus_tag="VFU96_10605"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012688043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU5113758.1"
                     /translation="ETAAYGLIIGGALGNLVDRVFRGPGLLDGKVIDWIDLWWIPTFN
                     VADMSITIAVTLLLIHAWLTRST"
     gene            183..1103
                     /locus_tag="VFU96_10610"
     CDS             183..1103
                     /locus_tag="VFU96_10610"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU5113759.1"
                     /translation="MADTLHLAVPERLAGERVDKVLAELLDLSRAQARALLDAGVRLD
                     GSPARPSDRVHFGAVVETPKPEVARVLAAEPADFGVIYGDRDLIVVDKPAGLVVHPGS
                     GHERPTLAAGLLHRYPELEGVGAPGRWGLIHRLDRDTSGLLVVARTAASFERLNTDMA
                     RRRIRRVYTTLVHGRFATPTGTIDAPIGRDPDRPTRRAVVAGGKPAVTHYEVVEEFPA
                     ADVSLLEVALATGRTHQIRVHMTAIDHPIVGDRVYSVLKKAVESPRIFLHAHHLSLRH
                     PASGEDLTFTSPMPPDLVGVLDLVRFPEDG"
     gene            1353..1958
                     /locus_tag="VFU96_10615"
     CDS             1353..1958
                     /locus_tag="VFU96_10615"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU5113760.1"
                     /translation="MTFIVVAVIVFLLGYVGYRDELVELFAQSNAAIADGEWWRIFSV
                     VLLHGSLTHIIFNMWALWVLGPQIERGVGTWPFVSLFLASAGVGGVFAYYLGGPTDIA
                     VGASGAIFGLFGIWLSWALHRRNTMQGRALLGQLIFLLLINAAIPFVFRTVSWQAHLG
                     GLIAGFLIGEIWSRIRGPNAQAMRAWVGFAVAVVAALAVAI"
     gene            2005..3447
                     /locus_tag="VFU96_10620"
     CDS             2005..3447
                     /locus_tag="VFU96_10620"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5113761.1"
                     /translation="MTIPRSILSRELLPASVAIFTTVAIVAFEGLAVTAALPDLTAEL
                     GQVHLLPWVITAFLLASGVATAIAGSFIDSVGTSTVFRWATLAFAASSLAAAAAGSMP
                     MLVATRVLQGAAGGAIISVGVGAVALVYPGHLVGRAFAANSNVWGILGIAGPAIAAAL
                     LQVGSWRWIFLLMVPISAVALIAGWRTLPGPIEPSPLRVDWLAVGLLVITFGSLLAAV
                     SNFSPFSLAFAGVSVLAGTVLWRRMAGGGSSLLDPRFVGGYPYRQLALVVSLTLVAMI
                     GLSSYLPVYVRGARGGTAAAAAWSVLWLTLGWTTAANVSGRITDRVSERAVLRVAAIG
                     GIPAIAAAWAAVAFSAPLPLVYASYFAMGMSVGAVTNSAFQMVRLAVSDNLAGRATSA
                     HAFLRTVGMSVGAGLAGGVILATVAASVGDISSVREALAGGEADLAGATANALARGFS
                     IAHGFSLLVMAVAAWMAGSLAQGRSDGTAG"
     gene            3468..>3957
                     /locus_tag="VFU96_10625"
     CDS             3468..>3957
                     /locus_tag="VFU96_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEU5113762.1"
                     /translation="MTAVSFAHLHVHTEYSMLDGAARIKDVVAAAAADGQPAIAITDH
                     GVMYGVVDFVKEAKASGVKPIIGMEAYLTEGSRFDRPVGNANRRFHMNLLAENEAGYR
                     NLLQLSSRAFLEGYYYKPRMDFELLAEHSEGIIATSGCLGGVVAQLLAPDSMSEEGNK
                     GQV"
BASE COUNT          560 a         1299 c         1307 g          791 t
ORIGIN      
        1 gagacggctg cctacgggct gatcatcggt ggcgccttgg ggaatctcgt cgacagggtg
       61 ttccgcggcc ccggtctgct cgatggaaag gtcatcgact ggatcgacct gtggtggatc
      121 ccgaccttca acgtggccga catgtcgatc acaatcgcgg tgaccctgct actgatacac
      181 gcgtggctga cacgctccac ctagccgtgc ccgaacgact ggcaggcgag cgggtcgaca
      241 aggtgctcgc agagctgctc gatctgagcc gggcccaggc gcgggcactc ctcgacgcgg
      301 gcgtccgact ggatgggtcg ccggctcggc ccagtgaccg tgtccacttc ggggcagtgg
      361 tcgaaacccc gaaaccagag gttgcgcgcg ttctcgccgc cgaaccggcc gacttcggag
      421 tgatctacgg agaccgcgac ctgatcgtgg ttgacaagcc ggccggtctg gtggttcatc
      481 ccggttcagg gcacgagcgg cccactcttg ctgctgggct gcttcatcga tacccggagt
      541 tggagggcgt gggcgcgccc gggcgctggg gcttgatcca ccgactcgat cgggacacct
      601 ccggtttgct ggtcgtggct cgcacggcgg cgtcgttcga gagactcaac accgacatgg
      661 cccggcgccg tatccgacgt gtctacacca ccttggtcca tggccgattt gccaccccaa
      721 ccgggacgat cgacgccccg atcggtcgtg atccggatcg gcccactcga cgtgccgtgg
      781 tcgccggtgg caaaccggcg gttacccatt acgaggtcgt cgaggaattc ccggccgcag
      841 atgtctcgct gctcgaagtc gctctggcca cgggtcgcac ccaccagatc agggtccaca
      901 tgaccgcgat cgatcacccc atcgtcggcg acagggtcta ctcggtcttg aagaaggcgg
      961 ttgaaagccc ccggatcttc ctccatgcgc accatttgag ccttcgtcac ccggcgtccg
     1021 gggaggacct cactttcacg tcgccgatgc cgcctgacct ggtcggtgta ctcgatttgg
     1081 tccggtttcc ggaggacggg taggccggtg acggcgatac cgttcccgtc ttgacccagg
     1141 accacaccac caccccggtg tgctatcggc accccgatcg gagcacattg ctcagttgca
     1201 gtcactgcgg ccggcccatc tgctccgcct gctccaccga cgcctcagtc gggcagcgct
     1261 gccccgactg catgagacag gaggggacac agcgtgtgat ccctaccggt ccccggagcc
     1321 gtcagtccgc gtcgtccggt gccccggcca cgatgacctt catcgtcgtc gcggtgatcg
     1381 ttttccttct tggctacgtc ggttacaggg acgaacttgt cgagttgttc gctcagagca
     1441 acgcggcgat cgccgacggt gagtggtggc ggatattcag cgtggtgctg ctccacggtt
     1501 cgctcaccca catcatcttc aacatgtggg cactctgggt gctcggtccc cagatcgagc
     1561 gtggggtggg gacctggccc tttgtcagtc tgttcctggc ctcggccggt gtgggtggtg
     1621 tctttgccta ctacctgggt ggccctaccg atatcgccgt cggtgcctcg ggagccatct
     1681 tcggcctctt cgggatctgg ctgagctggg ccctgcatcg aaggaacacg atgcagggaa
     1741 gggccctgct cggccagctc atcttcctcc ttctgatcaa tgccgccatc ccgttcgtgt
     1801 tcagaaccgt ttcgtggcag gcacacctcg gcggtctcat cgcagggttc ctgatcgggg
     1861 agatctggag ccggatacgc ggtcccaatg cgcaggcgat gcgggcctgg gtcggatttg
     1921 cggttgccgt cgtggccgca ctcgccgtcg cgatctgaga gaagacgtgc tcgcgcgttt
     1981 gaggttgcca tccttggtcg ggcaatgacc atccccaggt ccatcctctc ccgcgagctg
     2041 ctgccggcgt cggtcgccat cttcaccacg gtcgccatcg tcgccttcga gggtctggca
     2101 gtcaccgcgg cgctccctga cctcaccgcc gagttgggtc aggtccatct tctgccgtgg
     2161 gtgatcaccg ccttccttct cgcctcgggt gtggcgaccg caatcgccgg gtcgttcatc
     2221 gattctgttg ggacttccac cgtgttcagg tgggccactc tcgcctttgc cgcctcctca
     2281 ttggcggctg cggccgccgg atcgatgccg atgttggtgg ccacccgggt cctccagggc
     2341 gccgccggcg gggcgatcat ctcggtcggc gtcggcgcgg tggccctcgt ctacccgggc
     2401 catcttgtcg gaagggcctt tgccgcgaat tccaacgtct gggggatcct cggcatcgcc
     2461 ggccccgcca tcgccgcggc cttgctccag gtcggctctt ggcggtggat cttcctgctc
     2521 atggttccca tatctgcggt ggcgctgatc gccggatggc ggaccctgcc cggcccgatc
     2581 gagcccagcc cgctacgggt cgactggtta gccgtcgggt tgctcgtgat cacgttcggg
     2641 tccctcctgg cggcggtatc gaatttctct ccgttctctc tggccttcgc cggggtctcg
     2701 gtgttggccg gaaccgtgtt gtggaggagg atggccgggg gcgggtcgag tctgctcgac
     2761 ccgcgcttcg tcggggggta cccctaccga cagctcgcct tggtcgtctc gctgacattg
     2821 gttgcgatga tcgggctgtc gtcgtatctc ccggtctacg tgcgtggagc acgtggcggc
     2881 accgccgccg cagcggcgtg gtcggtgttg tggctcaccc tcggatggac gacggccgcc
     2941 aacgtctctg gtcggatcac cgatcgggtg agcgagcgag cggtgctccg agtggcagcg
     3001 atcggaggca tccctgccat cgccgccgcc tgggccgcag tcgcgttctc ggccccgctg
     3061 cccctcgtct acgcctccta tttcgccatg ggaatgtcgg tgggagcggt gaccaattct
     3121 gcatttcaga tggtgcgcct ggcggtatcg gacaacctgg ccggccgggc gacctcggcc
     3181 cacgccttcc tgcgcaccgt cgggatgtca gtgggggcgg gtctggcagg gggagtgatc
     3241 ctggccacgg tggcagcctc ggtaggggac atctcctcag tgcgggaggc gctggcaggt
     3301 ggtgaggcgg atctggccgg cgccacggcc aacgctctcg cccgcggctt ttcaatcgcc
     3361 cacggattca gcctgttggt tatggccgtc gccgcctgga tggccggaag cctcgcccag
     3421 gggcgatcgg acggcaccgc cgggtgaccc ggcctgtact aaggtgaatg acagccgtgt
     3481 cattcgccca cctccacgtc cacaccgagt attcgatgct cgacggtgcg gcccggatca
     3541 aggatgtggt cgctgccgcg gcggccgatg ggcagccggc gattgccatc accgatcacg
     3601 gggtcatgta cggggtggtc gatttcgtca aagaggccaa ggcctcgggc gtcaagccca
     3661 tcatcggcat ggaggcatat ctgacagaag ggtcgagatt cgaccggccg gtgggcaacg
     3721 ccaaccggcg cttccacatg aacctcctgg ccgagaacga agccgggtat cggaacctgc
     3781 ttcaactctc gtcgcgggcc ttcctcgagg gctactacta caagccgagg atggacttcg
     3841 aactgctcgc agagcactcc gaaggcatca tcgccacgtc ggggtgtctc gggggggtgg
     3901 tcgcacagct cctggctccc gactccatga gcgaggaggg gaacaagggc caggttc
//
LOCUS       DASTXO010000501         9549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_730699, whole genome shotgun sequence.
ACCESSION   DASTXO010000501 DASTXO010000000
VERSION     DASTXO010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 9549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9549
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730699"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VFU96_10630"
     CDS             complement(<1..275)
                     /locus_tag="VFU96_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006943907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HEU5113763.1"
                     /translation="MSLDLSGVDPLRIPRHVGLILDGNGRWANARGLPRTAGHAAGEA
                     ALFDTVEGALEIGIEWLTAFTFSTENWTRSDEEVEFLMWFNEDLLTRR"
     gene            complement(272..997)
                     /gene="recO"
                     /locus_tag="VFU96_10635"
     CDS             complement(272..997)
                     /gene="recO"
                     /locus_tag="VFU96_10635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU5113764.1"
                     /translation="MPSMAIRHDQGIVLRSYPFGEADRVVVLLSPNHGKIRTVAKGIR
                     KTKSRFGGRLEPFTHVDLVLYEGRNLDTITQVAVIEPFPRLRASLDAVVAAGTMVEAA
                     DAVAQEQESSMRLFLLLHRGLRALEAGQIGADLITSYLLKLADVVGVAPSLLVCASCG
                     RVDDLHRFSFGGGGAICDLCRTEGAVRLRDGVTAYLAALARAEFGQMPETDVALAGEA
                     MGIARRFVEYHLDRKLSSLAVME"
     gene            1050..1334
                     /locus_tag="VFU96_10640"
     CDS             1050..1334
                     /locus_tag="VFU96_10640"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019929325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEU5113765.1"
                     /translation="MAIVSDQEIDAFVEAHPGWGKVGDGIARTYELVDFNESMGLVTR
                     VALAAEKADHHPDIDIRWNKVTLTLSTHSEGGVTAKDLNLADLCDGLASS"
     gene            complement(1337..2224)
                     /gene="era"
                     /locus_tag="VFU96_10645"
     CDS             complement(1337..2224)
                     /gene="era"
                     /locus_tag="VFU96_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005885051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU5113766.1"
                     /translation="MRSGFVPVVGRPNVGKSTLVNAMVGSKVAITSSRPQTTRNAIRG
                     VVNGPGEDPEYQIVLIDTPGLHKPRNELGTRLNSMVYGTLAEADVVLFLIDATQAVGP
                     GDRLIAERLGEAEAEVIIVVSKLDIGRRGQVVEQLTKAGEWDFASYVPVSAIDGSGVP
                     ELVSEVVSRLPEGPVYYPPGVTTDQPESLVIGEIVREKFLDRLREELPHSLVVVVEDL
                     EQREDGLIDIAADVIVERRSQRGIVIGKGGSLLKEAGTEARHDLESLLGNRVNLALQV
                     KVEPDWQRKPQLLDRLGFD"
     gene            complement(2221..3408)
                     /locus_tag="VFU96_10650"
     CDS             complement(2221..3408)
                     /locus_tag="VFU96_10650"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEU5113767.1"
                     /translation="MNVAILIVAVAAMALAAWLRAAGSAISRVPRADALRDAADGVRG
                     AGTVAELLEEREGITPAVAMVGSAVLVVAAVAGTALVAAEQTLGTAITYAVVVFLIVF
                     LIGDLAPRQLGRRRPRSIAYRSARLLRVAVGLGGWASDLITEANGDDEDEIEPEPETV
                     TEQEREMIDSVLEFGDTVVREVMTPRPDMVTVPVTASVDELISVTTEEGFSRIPVTSN
                     GDVVGMVMVKDLLPLLGEGRRPASVAEVMRPVEFVPETKLASVLLGEMQANHQHQMIV
                     VDEYGEVAGLVTIEDLLEELVGEIADETDEEEIFISVRGDGGWDIDGRLPVDDLARLA
                     GVDLPDEDWDTVAGLVMGLAERVPDEGEQFSHGPLTLTVTRMQGRRVADVSVSLSEAV
                     DAE"
     gene            complement(3405..3869)
                     /gene="ybeY"
                     /locus_tag="VFU96_10655"
     CDS             complement(3405..3869)
                     /gene="ybeY"
                     /locus_tag="VFU96_10655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU5113768.1"
                     /translation="MSVFLADEQNEPVDLLRLRHLAELVLTEEGFPDETEVTVLLVSD
                     DEMASYNERFLDRSGPTDVLAFPVEELTPGFVPDQDPAGPPLVIGDVIVAPAYVKRQA
                     GETEVGFDDEMALMVAHGILHLLGYDHIEDGDAELMERRESELLSMVGVERR"
     gene            complement(3907..6417)
                     /locus_tag="VFU96_10660"
     CDS             complement(3907..6417)
                     /locus_tag="VFU96_10660"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEU5113769.1"
                     /translation="MPRPTLVRLATFVATVVAAWGVLSLGTGSADPDLVVGAPAPRDY
                     RAQRPNSVIDVAETQRLQQEARDAVDPIEQINEQIESTVNQNVTAVFDDVQALVMGDQ
                     VDPPAPAATEAPPTTAAEETTETTAATEPSVLTGTVFLDVESDGVFDPEAEGARVDTG
                     AERIAVTIQMGDETFEVMTDANGAWSAEVPAGAALVRIDPEDGQIPEGYVVGSPNYQQ
                     LVECESGDTCTAEPIPLHVNLRPVEEVIARVTATHTVASDAIATLVAAASDDVIRAAL
                     GEDPHFPLIRQAAVDRLFQEFGLRISPDQLQTAQQRLRSNPPQVVFLDTNPPGPDEAA
                     SDAAGEIVATFLQANYLVDEAATEDARAEAAEAVPDVMVEYVADQVIVARGVPVTQHH
                     IDAIDATTSAASQAEADGGLLAVLGVLVGLLGLYLSRFRTEFWARPRMVALFGILIVL
                     AAGAVRATVIFQPQSSWYILPAVAFGFMTAVLFDSRIAVLMALAVGVIAAVGTQDTGV
                     AVYATLATLAPIPFVSSVTSRGGFRTAVVFSALTAAVVAGATASFFHVGPDQVVWEVV
                     GTSMAWAFGISAIASLVGLAALQFFESAFDITTSLSLLDLTDRNHEALQLLQEKAFGT
                     FNHSLMVGTLAHAAARAIGANALLARAMAYYHDLGKTENPTMFIENQFGIQNPHDFLL
                     PLESAEVIRRHVSDGIVLARQFKIPSDVATGIVSHHGDGIMRFFYEKARAQEGDHVNP
                     NDFRHIGHKPRTAETAIVMLSDSLEAACRANFQTEEPTPEAIEKVVDRVIDEKLNDGQ
                     LSESPLTLSEMTLIRSAFLDSLVGHYHQRIAYPNFPGT"
     gene            complement(6417..7271)
                     /locus_tag="VFU96_10665"
     CDS             complement(6417..7271)
                     /locus_tag="VFU96_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU5113770.1"
                     /translation="MRFIARGDEVAFKGPDTEVERARTVLEELLILVQEGHALDSARV
                     DEVVKMVIEDVPSPSGVLSDGIPVGRGRMIRPKTHGQQKYMEAIRENTLTFGIGPAGT
                     GKTYLAMAAAVDSLVSGSVRRIILTRPAVEAGERLGFLPGDLAAKVDPYLRPLYDALW
                     DMLGPEETAALVEKGTIEIAPLAYMRGRTLNDACVILDEAQNTTPEQMKMFLTRLGFN
                     SKMIITGDMTQTDLPDNRRSGLKVVREILSDIPGVSFVELTARDVVRHRIVASIVEAY
                     ARFESKAN"
     gene            complement(7465..7878)
                     /locus_tag="VFU96_10670"
     CDS             complement(7465..7878)
                     /locus_tag="VFU96_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5113771.1"
                     /translation="MVDRFSATLGLRLRAARRQRGWSLGELESYTGGEFKASVVGAYE
                     RGERALSVQRLVRLAEIYAVPAADLLPLGASERGVLIDLDMAAESKDGDLIDRYLAAI
                     HFLRREGRPDEVRDSDKAVIASLLDSTVESVSGQD"
     gene            complement(7911..8555)
                     /locus_tag="VFU96_10675"
     CDS             complement(7911..8555)
                     /locus_tag="VFU96_10675"
                     /inference="COORDINATES: protein motif:HMM:NF016347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEU5113772.1"
                     /translation="MPHLLVPGPWIGDHLTLSSEQKHHLEKVLRVRDGEPASYTDGSG
                     TLGHGRYETGHLTRDEESRVPAPSDLIVVVAPPDSRERARFMVEKLSEMGVAELLFLG
                     TRHGQGRPPNAGKLLSWAVSGLEQSRGAWLMKTSDHQATFADLSPPFAVCDLGGTRVT
                     PLARTVVIGPEGGWGNEEVPEEGMRWSLGETVLRVETAALVAAARLLGNRDGNM"
     gene            complement(8577..>9549)
                     /locus_tag="VFU96_10680"
     CDS             complement(8577..>9549)
                     /locus_tag="VFU96_10680"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEU5113773.1"
                     /translation="NPGDPVSEARFRDAAEAYEVLSDPDRRRRYDRGDTIDLSDLFAG
                     FGGFDDLLRSVFGDNSLFGGAARARPNRGSDVLVRAEVDLLGAAFGTDAEVTFGTKDT
                     CEVCSGTGAEDGQGQTTCPQCGGAGSVRAARRSLFGTMMTVGTCPTCHGEGVLVTNPC
                     PACSGMGAVDGERTMTVEVPAGVSSGTRLRISGRGQSGGRYGPPGDLYVEVLVTPDPR
                     FERHDADLVHQVSIGIAEATLGTRLAIPLIEGGESDLEIPAGTQPGTVFRMKGLGVTH
                     LGRRTRGDLHVVVSVTVPSDLTRDEEEALRRWAELRGERTDRPASAG"
BASE COUNT         1719 a         3143 c         3023 g         1664 t
ORIGIN      
        1 cgccgggtga gtagatcctc gttgaaccac atgaggaact cgacctcctc gtccgagcgc
       61 gtccagttct cggtggagaa ggtgaaggcg gtcagccact cgatcccgat ctcgagcgcc
      121 ccttccaccg tgtcgaacaa cgcggcctca cccgcggcgt gtcccgcggt tcgtgggagc
      181 ccgcgagcgt tggcccatcg cccgttgccg tcgaggatca gcccgacgtg cctggggatt
      241 cgcagcgggt cgactccgga gaggtccaga ctcactccat caccgccagc gaggaaagct
      301 tccgatcgag gtggtactcg acgaagcggc gggcgatgcc catcgcctcc ccagcgaggg
      361 ccacgtctgt ctcgggcatc tgcccgaatt cggcccgggc tagggcggcc aggtaggcgg
      421 tgactccgtc gcgaagacgc accgccccct cggtccggca caggtcacag atggctccgc
      481 cgccgccgaa cgagaagcga tgcaggtcat cgacccggcc acaagaggcg cagacgagga
      541 gtgaaggcgc cacccccacc acatcggcca acttcaggag atagctggtg atgagatcgg
      601 cgccgatctg accggcttcg agggcgcgga gaccccggtg aagcagcagg aagagccgca
      661 tcgaagactc ctgttcctgc gccacggcgt cggcggcctc caccatggtg cccgccgcca
      721 ccaccgcatc gaggctggcc cgcagacggg ggaaaggctc gatcaccgcc acctgggtga
      781 tggtgtcgag gtttcgaccc tcgtatagga ccaggtcgac gtgggtgaac ggctccagac
      841 gcccgccgaa ccggcttttt gtcttgcgga tccccttggc caccgtgcgg atcttgccgt
      901 gattggggct gagcagcacc acgacgcgat ccgcttcccc aaaggggtag ctgcgaagga
      961 cgattccctg atcgtgacgg atggccatgg aaggcatcca gcgtaagcgc tggatgccgt
     1021 tctccgttgt acgttgtacg ttgctacgta tggcaatcgt ttcggaccaa gagatcgatg
     1081 cattcgtcga ggcccatccc gggtggggga aggtcggcga tggcattgcc cgcacctatg
     1141 agttggtcga cttcaacgag tcgatgggcc tggtgacacg ggttgccctg gccgccgaga
     1201 aggccgacca ccatcccgac atcgacatcc gatggaacaa ggtcaccctg accctctcca
     1261 ctcattcaga gggcggggta accgccaagg acctgaatct cgccgacctc tgcgacggtc
     1321 tggcctcgag ctgatctcag tcgaagccca gccggtccag aagctggggc tttcgctgcc
     1381 agtcgggctc gaccttgacc tggagggcca gattgacgcg attccccaac aaggattcga
     1441 ggtcatgccg tgcctcggtg ccggcttcct tgagcagcga cccacccttc ccgatcacga
     1501 tccccctctg gctcctgcgc tcgacgatta cgtcagcggc gatgtcgatg agcccgtcct
     1561 cacgttgttc gagatcctcg accacgacca ccagggagtg cggtagctct tcgcggagcc
     1621 ggtcgaggaa tttctcacgg acgatctcgc cgatcacgag gctctccggc tgatcggtcg
     1681 tcactccagg tgggtagtag accgggccct ccggcagacg ggatacgacc tctgagacca
     1741 gctccggtac tcccgagccg tcgatggccg aaaccggcac atacgaggcg aagtcccact
     1801 ccccagcctt ggtcaactgc tccacgacct ggccccggcg gccgatatcg agtttgctca
     1861 cgacgatgat gacctcggct tcggcttcgc cgaggcgctc tgcgatcagg cgatctccgg
     1921 gaccgacggc ctgtgtcgca tcgatcagaa agagaactac gtcggcctca gcgagtgtgc
     1981 cgtaaaccat cgagttcagc cttgtcccga gctcgttgcg cggtttgtgc agcccggggg
     2041 tgtcgatgag gacgatctga tactcgggat cttcgccggg gccgttgacg acgccccgga
     2101 tcgcatttcg cgtggtttgg ggccgcgagg aggtgatggc gaccttggag ccaaccatcg
     2161 cgttcacgag ggtggacttc ccaacgttgg gacgtcccac gacgggcacg aaacccgacc
     2221 tcattcggcg tcaacagcct ccgacagcga gaccgacacg tccgccaccc gcctgccctg
     2281 catccgggtg acggtgaggg tgaggggccc atgggagaac tgctcgccct cgtcggggac
     2341 gcgttcggcg agtcccatga ccagacctgc caccgtgtcc cagtcctcat cgggcaggtc
     2401 gacaccggcg agacgagcca gatcgtcgac aggaagacga ccgtcgatgt cccagcctcc
     2461 gtcgcctcgc accgagatga agatctcctc ttcgtcggtc tcgtcggcta tctctccgac
     2521 caactcttcg agcaggtcct cgatggtgac gagcccggcg acctccccgt actcatccac
     2581 gacgatcatc tggtgttggt gattggcctg catctcaccc agcaacaccg aggccagctt
     2641 ggtctccggg acgaactcga cgggtcgcat gacctctgcc acggaggccg ggcgacggcc
     2701 ctcccccaac agcggcaaca ggtccttgac catcaccatg cccacgacgt cgccgttgct
     2761 ggtcaccggg atcctcgaaa agccctcctc ggtcgtgacc gagatcagct cgtccaccga
     2821 cgccgtgacc ggcaccgtca ccatgtcggg acggggcgtc atcacctcac gaaccacggt
     2881 gtcgccgaac tcgagaactg aatcgatcat ctcgcgttcc tgctcggtga cagtttcagg
     2941 ctcaggttcg atttcgtctt cgtcatcacc gttcgcctcg gtgatcagat cggatgccca
     3001 gccgccgagg ccgacggcaa cacgaagcag cctggccgac ctataggcga tcgaacgcgg
     3061 tctgcgcctt cccagttggc ggggcgccag gtcaccgatc agaaacacga tcagaaagac
     3121 cacgacggcg tacgtgatgg cggtacccag ggtctgttcg gccgcgacga gagctgtccc
     3181 ggcaacagcg gccacaacca gcaccgccga tccgaccatg gccacagcgg gcgttatccc
     3241 ttcgcgctcc tcgaggagct cggccacggt gcccgcgccg cgaacaccgt cggccgcatc
     3301 acgcagagca tcggctctcg gaacacgact gatcgccgac cccgccgcgc gcagccaggc
     3361 ggcgagagcc atcgcggcga cagcaacaat caggatcgcc acgttcatcg ccgctccaca
     3421 ccaaccatcg acaacaactc ggattcccga cgctccatca actcggcatc tccgtcctcg
     3481 atgtggtcgt acccgaggag gtgcagaatg ccgtgggcga ccatcaacgc catctcgtcg
     3541 tcgaagccaa cttcggtctc gcccgcctgt cgcttgacat aggccggggc aacgatcaca
     3601 tcgccgatga cgagtggggg tccggcgggg tcttggtcgg gcacgaatcc cggtgtcaac
     3661 tcctcgaccg gaaacgcgag gacatcggtc ggcccggaac gatcgaggaa acgctcgttg
     3721 taggaggcca tctcgtcgtc gctgacgagc agcacggtca cctcggtctc gtccgggaat
     3781 ccctcttcgg tcaacaccaa ctcggcgaga tggcgcaacc ggagcaggtc gacgggctcg
     3841 ttctgttcgt cggccaggaa gacgctcact ggccggcgca gggttttcga ctgggggggt
     3901 cgtccatcag gtaccgggga agttgggata cgcgatccgc tgatggtaat gaccgaccag
     3961 ggagtcgagg aaagcggaac ggatcagagt catctcggac agggtcagcg gcgactctga
     4021 gagttggccg tcgttcagtt tctcgtcgat gacccggtcc accaccttct cgatggcctc
     4081 aggcgtcggc tcctcggtct ggaaattggc ccggcacgcc gcctccagtg agtcggacag
     4141 catgacgatg gccgtctccg ccgtccgggg cttgtggccg atgtggcgga agtcgttcgg
     4201 gttgacgtgg tcgccttctt gagcccgtgc cttctcgtag aagaagcgca tgatcccgtc
     4261 gccgtggtgg ctgacgattc ccgtggcgac gtctgagggg atcttgaact gacgggcgag
     4321 gacgatcccg tcgctgacgt gcctgcggat gacctcggcc gactccagcg gcagcaggaa
     4381 gtcgtgggga ttctgaatgc cgaattggtt ctcgatgaac atggtggggt tctcggtctt
     4441 tcccaggtcg tggtagtacg ccatggctcg agccagaagg gcattggcgc ctatcgcgcg
     4501 ggcggcggca tgggccagcg tccccaccat cagcgagtgg ttgaaggtcc cgaacgcctt
     4561 ctcctgtagc aactggagcg cctcgtggtt gcgatcggtc agatcgagga ggctgagact
     4621 cgttgtgatg tcgaaggccg attcgaagaa ctgaagggca gccagaccca ccagcgacgc
     4681 aatggccgag atgccgaatg cccatgccat cgaggtgccg actacctccc acacgacttg
     4741 gtccggaccg acgtggaaga aggacgctgt ggctccggcg acgactgcag cggtgagtgc
     4801 agagaacacg acagcggtcc ggaagccgcc ccgactggtc accgagctca caaatgggat
     4861 cggggcaagc gtggcgaggg tggcatagac ggctacgccg gtgtcctggg ttccgaccgc
     4921 ggcgatcacg ccgacggcca atgccatgag cactgcgatt cgggagtcga agagaaccgc
     4981 cgtcatgaat ccaaatgcga ccgccgggag gatgtaccaa ctcgactgag gctggaagat
     5041 gacagtcgcc cgaaccgcac ctgcggcaag cacgatgagg atgccgaaaa gggcgaccat
     5101 gcgtggccgg gcccagaact cggtgcggaa tcgcgagaga tacaggccga ggaggccgac
     5161 caacacgccg agaacggcga gcagaccgcc atccgcctcg gcctgggatg ccgccgaggt
     5221 ggtggcatcg atggcgtcga tatggtgctg tgtcaccgga actcctcggg cgacgattac
     5281 ctgatcggcg acgtattcga ccatgacgtc ggggacggcc tcggctgcct cggccctggc
     5341 gtcctcggtg gccgcctcat cgaccagata gttggcctgg aggaatgtcg ccacgatctc
     5401 acctgcggcg tcacttgccg cctcgtcagg accaggggga ttggtgtcca ggaagaccac
     5461 ctgtgggggg ttggagcgga gacgctgttg ggccgtctgc aactggtctg gggaaatcct
     5521 cagtccaaac tcctggaaga gccgatcgac ggcggcctgt cggatcagcg gaaagtgggg
     5581 gtcctcgccc agggccgccc tgatcacgtc gtcactggcg gcggcgacaa gtgtcgcaat
     5641 cgcatcgctg gcgacggtgt gggtggccgt cacccgggcg atgacctctt cgaccgggcg
     5701 gaggttcacg tgcagcggga ttggctcggc agtgcaggta tcgccagatt cgcattcgac
     5761 cagctgctgg tagttggggc tcccgacgac gtagccctcc gggatctggc catcttccgg
     5821 gtcgatgcgc accagcgcgg caccggcagg gacctcagcc gaccaggcgc cgttggcatc
     5881 ggtcatcacc tcgaaggtct cgtcgcccat ctggatcgtc actgcgatcc gctcggcccc
     5941 ggtgtccact cgtgcccctt ccgcctccgg gtcgaacacg ccgtcagatt ccacgtcgag
     6001 gaacaccgtc ccggtcagaa ccgaaggctc ggtagccgcg gtggtctcgg tcgtctcctc
     6061 ggccgcggtc gtcggcgggg cctccgtggc tgcaggtgcg ggcggatcaa cctgatcacc
     6121 catcaccaag gcctggacat cgtcgaagac ggctgtcacg ttctgattga cggtgctctc
     6181 gatctgctcg ttgatctgct cgatcgggtc gaccgcatcc ctcgcctcct gctgcagacg
     6241 ctgggtctcg gcgacgtcga tcacggaatt tgggcgctgg gcccggtagt cacgcggcgc
     6301 tggggcgccc accaccaggt ccggatctgc ggatccggtg cccagggaga ggactcccca
     6361 cgctgcaacc acagtggcca cgaatgtcgc aagacgcacc agagtcggcc gcggcatcag
     6421 ttggccttgc tctcgaacct ggcgtaggcc tcgacgatcg aggcgacgat gcgatgccgg
     6481 accacgtcac gagcggtcaa ttcgacgaaa gaaaccccgg ggatgtcgct caggatctca
     6541 cgcacgacct tgagaccgga gcgacggttg tcggggaggt cggtctgggt catgtccccg
     6601 gtgatgatca tcttcgagtt gaagccgagc cgagtaagga acatcttcat ctgctcgggc
     6661 gtcgtgttct gagcttcgtc gaggatgaca caggcgtcgt tgagggtcct tcctctcata
     6721 taagcgagcg gtgctatctc gatagttccc ttctcgacta gcgctgccgt ctcctcgggg
     6781 ccgagcatgt cccacagggc gtcatacaac ggccgcaggt agggatcgac tttggcggcg
     6841 aggtctccgg gcaggaatcc gagcctctcc cccgcctcca cggcggggcg ggtcaggatg
     6901 atgcggcgaa ccgagccact gaccagactg tccacagcag cggccatggc caagtaggtc
     6961 ttcccggtgc cggcgggccc gatgccaaag gtcagcgtgt tctcccttat ggcctccatg
     7021 tacttctgct ggccatgggt cttgggacgg atcatccgtc ctcgccccac cgggattccg
     7081 tcagagagca cgcccgaggg gctgggcacg tcttcgatga ccatcttcac cacttcgtcg
     7141 acccgggccg aatcgagcgc atggccctcc tggacgagga tcagcaactc ctcgaggacg
     7201 gttcgggcac gctcgacctc ggtgtcgggg cctttgaacg ccacctcgtc cccgcgcgcg
     7261 atgaatcgca cctccggaaa atgtgattcg acctgacgga ggtgtctgtc ggcctcgcca
     7321 aggagatcca gcatcaaatg gtggctgggg acgcgtaaac gcgagtcgat agattcggtg
     7381 ggttgctcag tgggcacagg agatgacgcc ctctctgtgg cgtcgcgccc attgtagagg
     7441 gcgcccggtg ctgattcaga acattcagtc ctgcccggag acggattcca ccgtcgagtc
     7501 cagcagcgaa gcaatcactg ccttatccga gtcgcggacc tcgtcggggc ggccctcacg
     7561 gcggaggaag tggattgccg ccaggtagcg atcgatcaga tcaccgtcct tggactcggc
     7621 tgccatgtcc agatcgatca gcacgccacg ctccgacgcg ccgaggggca ggaggtcggc
     7681 tgccggcact gcgtagatct cggcgagacg caccaagcgt tgaacactca gcgcgcgctc
     7741 cccccgctca tatgcgccga caacggacgc cttgaactcg ccaccggtgt aggactcgag
     7801 ctcccccaat gaccaaccac gctggcggcg ggcggcacgt aagcgcaagc ccagcgtggc
     7861 gctgaatcta tcgaccaagg gtctcctccc gtaagcccgt gggcgagatg ctacatgttc
     7921 ccatcgcggt ttcctaggag cctcgccgcg gccaccagag ccgccgtttc gaccctgagc
     7981 acggtctcgc cgagactcca tcgcatcccc tcttccggga cttcttcatt gccccagccg
     8041 ccctcaggcc cgatgaccac tgtccgcgcg agcggtgtca cccgtgttcc tcccagatcg
     8101 cacaccgcga aaggcgggct cagatcggcg aaggtcgcct gatggtccga tgtcttcatc
     8161 agccacgccc cccgggactg ctcgagaccc gacactgccc aggaaaggag cttccccgca
     8221 ttgggcggcc gcccctgccc gtgccttgta ccgaggaaca gcaattcggc tactcccatc
     8281 tcggacaact tctcgaccat gaagcgggcc cgttcgcgac tatcgggcgg tgcaaccacg
     8341 acgatcagat cggaaggggc agggacacgg ctttcctcgt ccctcgtcag atgaccggtc
     8401 tcatagcgcc catggcccaa ggttccggat ccgtcggtgt aggaggcggg ctccccgtcc
     8461 cggactcgca gcaccttctc gagatggtgt ttctgctccg atgacaatgt gagatggtcc
     8521 ccgatccatg ggcccgggac cagaagatgt ggcacgtggc ccatcgaacg aatggatcag
     8581 ccggccgacg cgggccggtc cgtcctctca ccacgcagct ccgcccatcg ccgaagagct
     8641 tcttcctcgt ctctggtcag atcactgggg acggtgacag agacgacgac gtggaggtct
     8701 cctctcgtcc gccggccgag atgggtgacg ccgaggcctt tcatgcggaa aacggtcccg
     8761 ggctgagttc ccgccgggat ctcgagatca ctctcgccac cctcgatcaa cgggatagcg
     8821 agcctggtcc ccagggtggc ttcggcgatg ccgatcgaca cctggtgcac caagtcggcg
     8881 tcgtgacgct cgaatctcgg gtcgggtgtg accaggacct cgacgtagag atcgccgggc
     8941 ggaccgtaac ggccgcctga ttgtccacgg ccagagatcc ggagtcgggt cccggaggac
     9001 acaccagccg gcacctcgac ggtcatcgtc cgctccccgt ctacggcgcc catcccggaa
     9061 cacgccggac aggggttcgt gaccagcacg ccctcgccgt gacaggttgg gcatgtgccg
     9121 acagtcatca tcgtcccaaa gagggatcgt ctggccgccc gcaccgatcc cgctccgccg
     9181 cactgaggac atgttgtctg accttgacca tcctcggccc cggttccgga gcagacctcg
     9241 catgtgtcct tggttccgaa agtgacttcg gcgtcggtgc cgaaggcggc accgaggagg
     9301 tcgacctcgg cgcgcaccag gacgtcgctc cccctgttcg gtcgggcccg agccgcaccg
     9361 ccaaacaacg agttgtcgcc gaacaccgat cgcagaaggt cgtcgaaccc cccaaagcct
     9421 gcaaacaggt cggagagatc gatggtgtcc cctctgtcgt atcgacgtct ccggtcaggg
     9481 tcggacaaga cctcgtatgc ctcggcggca tcgcgaaatc gagcctcgga gacgggatca
     9541 cccggattc
//
LOCUS       DASTXO010000502         2352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_951990, whole genome shotgun sequence.
ACCESSION   DASTXO010000502 DASTXO010000000
VERSION     DASTXO010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951990"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VFU96_10685"
     CDS             <1..811
                     /locus_tag="VFU96_10685"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5113774.1"
                     /translation="ARGPQHTTPETSAGDWRLARDLGIRITVHAGEGAWSLRHQPVLR
                     LHEQGVLGPDTTFVHGNQFTDQDFEMIADSGGSISISPEVEMHMGLGFPITGKSLAHG
                     IVSTLSVDVPVTVGGDMFSVMRCALATERALVNEESIRRVEMVERLTISSSDILRMAT
                     IDGARSCGLGERTGSITPGKEADIILMRADDLNLAPLNQPVAAVVVMSHPGNVDSVLV
                     GGRFVKKSGQMLSVDTAAVLKKAIESRDSLLDRAGVSPDYRPDDIEAWSKL"
     gene            complement(828..1820)
                     /locus_tag="VFU96_10690"
     CDS             complement(828..1820)
                     /locus_tag="VFU96_10690"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5113775.1"
                     /translation="MTAVSEADAPSGARGWMGTIRRHQSIVGPWAILLLLVIVSTVIS
                     PDFLSTTNLLNILRQAAPLMIVALGQTLVILVGGIDVSVGALIATTTVIGGSLMRDSD
                     ALILPTVLIVIGVGVVVGLIHYVFIVRFRTDALVTTLGTMLILTGLNLIYTGGAPRSN
                     VPDLFRQLSEGRLIGVPIIVIIALALAATLAYVLRRSTFGRRVYAVGSNRSAARLAGI
                     RAHRVEGLAYVACSILAVLAGLALLARIGTGDTAAGEGMELDAIAAVLIGGTAFGGGK
                     GRIEGTVAGVLILTVLFNIFNLMGISRFAHLIVKGVVVIVGVALYSTRPRRGRG"
     gene            complement(1817..>2352)
                     /locus_tag="VFU96_10695"
     CDS             complement(1817..>2352)
                     /locus_tag="VFU96_10695"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113776.1"
                     /translation="FGPVPMLFFLYVVVAAVGLIVQNKTAFGTHLFAVGGSEQVARAS
                     GVRIARVHVAAYVACGFLAALAGVAMSMRMGAGEPLSGVGFDWDSIAAVVLGGTALTG
                     GRGGIGGTIGGVAIISILNNVMNLAGVSSFTQIVIKGIIVLIAVLLAAGVTRRVAEGV
                     GARLHRRSTPLEGDVAA"
BASE COUNT          461 a          781 c          751 g          359 t
ORIGIN      
        1 ggcccgtggc ccccaacaca cgaccccgga gaccagtgcc ggtgattggc gactcgcccg
       61 ggatctgggg atacgaatca ccgtgcacgc cggcgaggga gcctggtcgc tacgtcatca
      121 gccggtgctt cggcttcacg agcagggagt cctgggaccc gacaccacat tcgtccatgg
      181 caaccaattc acggaccagg acttcgagat gatcgccgac agcggcgggt cgatctcgat
      241 ttcgccggag gtcgagatgc acatggggct cggcttcccg atcacgggga agtcactcgc
      301 ccacggcatc gtctccacgc tctcagtcga cgtcccggtc acagtcggtg gcgacatgtt
      361 ctcggtgatg cgttgtgccc tggccaccga gcgggccctg gtgaacgagg agtcgatccg
      421 ccgtgtcgag atggtggagc gtctgaccat cagctcgtca gacatcctgc gaatggccac
      481 catcgacggg gcccgctcct gcggcctggg ggaacgcacc ggcagcatca ccccgggaaa
      541 ggaggccgac atcatcctca tgcgtgccga cgacctgaac ctggcgccgc tcaatcagcc
      601 ggtggccgcc gtggtggtga tgtcgcaccc gggaaatgtc gattcggtct tggtcggcgg
      661 caggttcgtc aagaaaagcg gtcagatgct ctcggtcgac actgcggcag tgttgaagaa
      721 ggcgatcgag tcgagggact ccctgctgga tcgggccggg gtcagccccg actatcggcc
      781 ggacgacatc gaggcctgga gcaagctctg acctcggtcg gggtggatca gccccgaccc
      841 cgacggggcc gggtggagta gagggccaca cccacgatca cgacgacacc tttcacgatc
      901 aggtgggcga agcggctgat ccccatcagg ttgaagatgt tgaacaacac ggtgaggatg
      961 agaacaccgg ccacggttcc ctcgatcctt cccttgcccc ctccgaacgc agtcccaccg
     1021 atcagcaccg cggcgatcgc atcgagctcc attccttccc cggccgcagt gtccccggtg
     1081 ccgatccgcg ccagcaacgc caggccggca aggacggcga ggatgctgca ggccacgtaa
     1141 gccaagccct cgacccggtg tgctcgaatc cctgccaggc gggctgcgct ccgattgctc
     1201 ccaactgcat agaccctgcg cccgaaggtc gagcgacgaa gcacataggc gagagtggcg
     1261 gcgagagcga gagcgatgat cacgatgatc gggaccccga tgagcctgcc ctcggacagc
     1321 tggcggaaca gatcggggac gttgctgcgg ggagccccgc cggtgtagat gagattgagc
     1381 ccggtgagga tcagcatcgt gcccagcgtg gtgaccaagg catcggtgcg gaaccgcacg
     1441 atgaacacat agtggatcaa tccgacgacc actccgacac cgatcacgat caacaccgtg
     1501 gggaggatca gggcgtcact gtctcgcatc aggctgccac cgatcaccgt cgtggtggcg
     1561 atgagggccc ccaccgagac atcgatgcca ccgaccagga tcaccagtgt ctgtcccagc
     1621 gccacgatca tcaggggcgc cgcctgtctg aggatgttga gcaggttggt cgtgctcagg
     1681 aagtcgggcg agatgacggt cgagacgatg accagcaaca gcaggatggc ccagggcccc
     1741 actatcgact gatgccgacg gatggtcccc atccatcccc tggcgccgga cggagcgtcg
     1801 gcttcgctga ccgcggtcac gctgcgacgt ctccttccaa tggtgtcgac cgacgatgca
     1861 gccgagcccc tactccttcg gcgacccggc gggtgacgcc ggccgccagc aggacggcga
     1921 tcaagacgat gatccccttg atcacgatct gggtgaacga ggacacaccg gccagattca
     1981 tcacattgtt gaggatcgag atgatcgcta ccccgccgat ggtgcccccg atcccacccc
     2041 ggcccccggt cagagcggtg ccgccgagga ccacggctgc gatcgagtcc cagtcgaaac
     2101 ccacccccga tagcggctcc cccgccccca tgcgcatcga cattgcgacc ccagccaggg
     2161 cggcgaggaa tccacaagcc acataggcgg cgacgtggac cctggcgatg cgaacccccg
     2221 aggctcgggc gacctgttcg gacccaccga cggcgaacag atgggtaccg aatgccgtct
     2281 tgttctgcac gatgagtccc actgccgcca ccaccacata gaggaagaag agcatcggga
     2341 ccggtccgaa gg
//
LOCUS       DASTXO010000503         2404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_915060, whole genome shotgun sequence.
ACCESSION   DASTXO010000503 DASTXO010000000
VERSION     DASTXO010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915060"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFU96_10700"
     CDS             <1..297
                     /locus_tag="VFU96_10700"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HEU5113777.1"
                     /translation="SGVGLGGTLITAKVGDFLERSTGEFDLVFIDPPWDMASSDLGMD
                     LTALDRLLRARAEVILSRRHTDTLPGAPPNWSVATDRRYGDTRIVRYETEAEDE"
     gene            294..761
                     /gene="coaD"
                     /locus_tag="VFU96_10705"
     CDS             294..761
                     /gene="coaD"
                     /locus_tag="VFU96_10705"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019355808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU5113778.1"
                     /translation="MTTALCPGSFDPPTNGHVDVIERAIAAFDRVVVAVVDNPSKESL
                     FSVAERISLLEELFGGRAEIGSFTGLLVEYATQVGADVVVKGLRTVDDYEYEMQMSQM
                     NRHLTGMETVFMATRSDWSFVSSSLVKEVAKLGGSIDGLVPSAVEKALQERLT"
     gene            758..1306
                     /locus_tag="VFU96_10710"
     CDS             758..1306
                     /locus_tag="VFU96_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113779.1"
                     /translation="MTDEQEAHLAEAVESMEVGPESRAELSDLQDTVDDLIVYLHEAK
                     NVPLSGNAMVDREQFLGMLEKIRADLPDELRAARWMVREREAFIARTNEKAREIVDRA
                     RNEADALVSDSNIMAEAVEEANILVRRAEGEARRLRLETEDEIERKLQRVELILNQVL
                     DQIQDARDELHEARPKPPDVPL"
     gene            1308..1799
                     /locus_tag="VFU96_10715"
     CDS             1308..1799
                     /locus_tag="VFU96_10715"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEU5113780.1"
                     /translation="MTHDLVVPVGDLIGSPGRDRPFAGEVTLSLDFGDSRVDGPMTLS
                     GRVVGLIDAVEAEFTASASAHFTCTRCLTEWDGPVEAASKRYFRRLPDEDGYAIEEDE
                     VDISGPARDELALALPATPLCRADCRGLCPTCGTDLNSEPCGGHGDDSDSPFATLKDL
                     FDS"
     gene            1815..1991
                     /gene="rpmF"
                     /locus_tag="VFU96_10720"
     CDS             1815..1991
                     /gene="rpmF"
                     /locus_tag="VFU96_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU5113781.1"
                     /translation="MAVPKKKMSRSRTRQRKSAWKVSRPSSVACEQCGKPKLPHRACR
                     ECGTYGGREVIEAS"
     gene            1995..>2404
                     /locus_tag="VFU96_10725"
     CDS             1995..>2404
                     /locus_tag="VFU96_10725"
                     /inference="COORDINATES: protein motif:HMM:NF014554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEU5113782.1"
                     /translation="MARIALDAMGGDNAPEETVAGAVDAAERGVEVVLVGVPQVIEAQ
                     LARHGTRLPIVDAPEVIGMGEDPARALREKPGASINVAAKLVEEGNAEGLVSAGSTGA
                     ATAAAAIHIGRLKGVRPSIASIFPTPGSPTLVLDS"
BASE COUNT          442 a          755 c          814 g          393 t
ORIGIN      
        1 agcggagtgg gtctcggtgg cacgctgatc acggccaagg tcggggactt cctcgaacgc
       61 tcgaccgggg agttcgacct ggttttcatc gacccgccct gggacatggc atcgtcggat
      121 ctgggcatgg acctcacggc actcgatcgg ttgctgcggg cccgggcgga ggtgatcctg
      181 tccaggcggc acaccgacac cctccccggg gcgccgccaa actggagtgt tgccaccgat
      241 aggcggtacg gggataccag aatcgtcagg tacgaaacgg aagctgaaga cgaatgacca
      301 ccgcactctg tcccgggagc ttcgaccccc ccaccaacgg gcacgtcgac gtcatcgagc
      361 gcgccatcgc cgcctttgac cgggtggtgg ttgcggtcgt cgacaaccct tcgaaggaat
      421 cgctgttctc ggtcgcggag cggatttcgt tgctcgagga actgttcggc gggagggcag
      481 aaatcgggtc cttcacgggc ctcctcgtcg agtacgccac ccaggtcggg gccgatgtcg
      541 tggtgaaggg cctgcgcacc gtcgacgact acgagtacga gatgcagatg tcccagatga
      601 accggcacct cacggggatg gagaccgtgt tcatggcgac caggtcggat tggagcttcg
      661 tctcgtcgtc actggtcaag gaagttgcca aactgggtgg atcgatcgat ggcctggtac
      721 caagtgccgt cgagaaagcc ctccaggagc gtctcacatg accgacgagc aggaagccca
      781 tctagccgag gcggtcgagt caatggaggt cgggccggag agtcgggccg agctgagcga
      841 tctccaggac acggtggacg atctgatcgt ctacctgcac gaggccaaga acgtcccgtt
      901 gtccggcaat gccatggtcg accgggagca gttcctaggg atgttggaga agattcgcgc
      961 cgacctgccc gacgagctgc gggcggcgcg gtggatggtt cgggaacgtg aggcgttcat
     1021 cgcccgcacc aacgaaaagg cccgcgagat cgtcgatcgt gcccgcaacg aagccgacgc
     1081 cctcgtctcg gattcgaaca tcatggccga ggcggtcgaa gaggcgaaca tcctggtgcg
     1141 gcgggccgag ggggaggcgc gccggcttcg gctcgagacg gaagacgaga tcgagcggaa
     1201 gctgcagcgg gtcgagttga tcctcaacca ggtcctcgac cagatccagg atgcccggga
     1261 cgagttgcac gaggcccgtc ccaagccgcc cgacgtcccg ctctgacatg acgcacgacc
     1321 tcgtggtccc ggtcggcgac ctgatcggat cgccgggcag agaccgtccc ttcgcgggcg
     1381 aggtcacgtt gagcctcgat ttcggagatt cccgggtcga tgggccgatg acgctctcgg
     1441 gacgggttgt cgggctcatc gacgccgtcg aggccgagtt caccgcctcg gcctcggctc
     1501 atttcacgtg taccagatgt ctcaccgagt gggacgggcc ggtcgaagcc gcttccaagc
     1561 ggtactttcg gagactgccc gatgaggacg ggtatgccat cgaggaagac gaggtcgaca
     1621 tctccggtcc cgcccgcgac gagctcgcgc ttgcgcttcc ggcgacacca ctctgccgag
     1681 cggattgccg gggactttgc ccaacctgtg gaaccgactt gaatagtgaa ccttgcggag
     1741 gccacgggga tgattccgac tcaccctttg ccaccctgaa agatctgttc gattcctgac
     1801 caaggagagc accaatggcg gtgcccaaga agaaaatgtc gcgctcgcga acgaggcagc
     1861 gcaaatcagc gtggaaagtg tcccgcccga gctctgtggc ctgcgagcaa tgcggcaagc
     1921 ccaagctgcc ccatcgcgcc tgtcgtgagt gcggcaccta cggcggccgc gaggtgatcg
     1981 aggcttcctg agccttggcc cggatcgccc tcgacgccat ggggggcgac aacgcccccg
     2041 aggagaccgt tgccggtgcg gttgacgccg ccgaacgcgg cgtcgaagtg gttctcgtcg
     2101 gggtccccca agtcatcgaa gcccaactcg cccgacacgg cactcggctt cccatagtcg
     2161 acgcccctga ggtgatcggg atgggggaag accccgcccg cgccctccgc gagaagccgg
     2221 gagcgtcgat caacgtggcg gcaaagctgg tcgaggaagg caacgcagag ggcttggtct
     2281 cggccggatc caccggcgcc gcgacggcgg cggcggcgat ccacatcggg aggttgaagg
     2341 gcgtgcggcc gtcgatcgca tcgatattcc ccactccggg cagccccact ctggtcctcg
     2401 actc
//
LOCUS       DASTXO010000504         4413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_141701, whole genome shotgun sequence.
ACCESSION   DASTXO010000504 DASTXO010000000
VERSION     DASTXO010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141701"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1334
                     /locus_tag="VFU96_10730"
     CDS             <1..1334
                     /locus_tag="VFU96_10730"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5113783.1"
                     /translation="DVLAGRDVAGRAPTGSGKTLAFGLPALARLGRGRSRRPRGLVLS
                     PTRELADQILAELQPFARAIGRSMLAVYGGVSYGPQRSSLQQGIDLLVACPGRLEDLI
                     AQGVVSLGEVDLVIVDEADRMADMGFLPAVRRVLDLTAPDRQTILFSATLDGDVATLT
                     HRYQRDPVRHESELQDEDAGSAEHHFWKVDRADRTAHVAKVVTSASPSIVFTRTRHGA
                     DRLAKQLAGAGVSAVALHGGRSQQQRNRALKDFSSGRSQALVATDVAARGIHVDGVAS
                     VIHFDPPEDEKAYVHRSGRTARAGAGGMVVSLVDGSQVKSAERLQRSLGIVSPMVTGP
                     AAGGISERVSVTTSRRPERQGHSGDSESIYVGNLPFSVTGKDLEQIFARHGSVRSATV
                     VTHRDSRKSRGFGFVEMTPKASNGAIAALDGTSIAGRRLKVRAARPGVAHP"
     gene            1345..2130
                     /locus_tag="VFU96_10735"
     CDS             1345..2130
                     /locus_tag="VFU96_10735"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU5113784.1"
                     /translation="MIEAAAVSRWKFIVIGLAAGLLSGGFGIGGGIVIVPLLVAVGLD
                     RHRAHATSLASIFPIAAAGAVTFALSGEVNLGLGVLVGVGGVIGSVVGASLMNRLSTK
                     SLSIFFGLVLLAAGIRMIFSTDPLPVASGFDDLMLGLIAVGIGLLSGFLAGVAGIGGG
                     IVIVPATVLLLGFTQHEAQGTSLLAIILTSVAATIINRKNRRVRLREAVLVGAGGMVG
                     AVLASVAALGVDGRVLSALFGAFAVLVAVRTLYQAYTTEPQPV"
     gene            complement(2127..2708)
                     /locus_tag="VFU96_10740"
     CDS             complement(2127..2708)
                     /locus_tag="VFU96_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEU5113785.1"
                     /translation="MISYVTSTALVVVDVQNDFADPNGSLFVQGGDAVVPRVNIEVMR
                     ALSAGAFVVYTQDWHPESTPHFEKDGGIWPVHCVQGTWGAEFHPGLEVSGPVVKNGAN
                     GEDGYSGFTMRDPVTGKETPTALRGMLDERGCQRVVVVGLALDWCVKETALDAAKDYD
                     TFVIPDATASVDLEPGDGQRAIEEMRRHGVTID"
     gene            2745..4256
                     /locus_tag="VFU96_10745"
     CDS             2745..4256
                     /locus_tag="VFU96_10745"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEU5113786.1"
                     /translation="MGISRGALLTDQYQLTMSQLYFRHGLHERDARFEHFFRRYPDYG
                     DHEAGYAINAGWGWLGRWADQARFGTEERDALRSHQVEGHRIFSDDFIEWLGGVSWDS
                     LRYEAIPEGRVAHPNVPMTVVEGPLAVAQILETSLLNHLNFHTLVATKASRVVEATRG
                     GAVLEFGLRRAPDEGGIAATRAALIGGANASSNVGISHEMGVMPQGTHAHSMVQVFMA
                     LGDGELGAFRAYADMYPDECVLLVDTIDTLASGIPNAITVFEELRVRGHRPIGIRLDS
                     GDLAHLSVLASQMLDRAGFEDVRIVLSSGLDEMTIFQILTQIADEAPRYGADPDRVIR
                     RLLYGVGTKMATSDGRPYLDGVYKLVAIADEEGWRPAIKVSDTPSKIMNPGRKRVHRL
                     YDQRGLATVDLMALADENLKFPLEVRHHRETGIGRTLDESSVSATEELLEPMTLAAPS
                     DDPSVVAEARKRRVSDLERLDPGVKRLVNPHVYHVSVTRRLADLKTRLIEETL"
BASE COUNT          740 a         1440 c         1427 g          806 t
ORIGIN      
        1 ccgatgtcct cgccgggcgt gacgtcgccg gtcgcgctcc gaccgggtcg ggcaagaccc
       61 tcgcctttgg gctccccgcc ctggcgcggc tcgggcgcgg caggagccga cgcccccgcg
      121 gtctggtcct gtcaccgacc agggagctgg ccgaccagat cctcgccgag ctccaaccgt
      181 ttgccagggc gataggccgt tccatgctcg ccgtgtacgg cggtgtctcc tacggccccc
      241 aacgctcgag tctccagcag gggatcgatc tgctggtcgc ctgccccggg cgtctcgagg
      301 atctgattgc ccagggagtc gtcagcctgg gagaggttga cctcgtcatc gtcgacgagg
      361 cggaccggat ggcggacatg ggatttcttc ctgcggtcag acgtgtcctc gacctgaccg
      421 cccccgaccg tcagaccatc ctcttctcgg cgacgctcga cggagacgtc gccactctga
      481 cccatcgtta ccagcgcgat ccggtgcgac acgagtccga gctgcaggat gaggatgccg
      541 ggagcgccga gcaccatttt tggaaggtag accgggcaga tcggaccgcc catgtcgcca
      601 aggtggttac ctcggccagc ccttcgattg tcttcacgag aacccggcac ggggccgatc
      661 ggctggccaa gcagttggcg ggcgccggtg tcagcgcggt tgcgctacac ggtggcagat
      721 cccagcagca gcggaaccga gctctgaagg acttctccag tggtcgcagc caggctcttg
      781 tcgccactga cgtggcggcg cgaggcatcc atgtcgatgg agtcgccagt gtcatccact
      841 tcgacccgcc ggaggatgag aaggcctatg tgcaccgttc ggggcgcacc gcccgggccg
      901 gagccggtgg catggtggtc tccctcgtcg atgggagtca ggtcaagagc gccgagaggc
      961 tgcagcgatc cctgggcatc gtctccccga tggtgaccgg tcccgccgcg ggcggaatct
     1021 cagaacgggt cagcgttacc acttcgagga gacccgaacg acaaggccac tcgggtgaca
     1081 gcgagtcgat ctacgtaggc aacttgccgt tcagtgtcac cggaaaggac ctcgagcaga
     1141 tctttgcccg gcatgggagc gtccgctcgg ccactgtcgt cacccatcgg gactccagga
     1201 agtcacgtgg attcggtttc gtggagatga ctcccaaggc ctcgaatggc gccattgccg
     1261 ctctcgacgg cacgagcatc gccggacgtc ggttgaaggt gagggcagcc agacccggag
     1321 tcgcccaccc ctaacctgcc cccggtgatc gaggccgcgg cagtatcgcg ctggaaattc
     1381 atcgtcatcg gacttgccgc cggcctcctc tcaggaggat tcggtatcgg cgggggcatc
     1441 gtgatcgttc ccctgctggt tgcggtcggc ttggaccgtc atcgcgccca cgcgacatcc
     1501 ctggccagca tcttcccgat cgcggctgca ggggcagtga cctttgccct ctcgggtgag
     1561 gtgaatctgg gtcttggtgt gctcgtcggg gtgggtggcg tgatcggctc ggtcgtgggt
     1621 gcctcgctca tgaatcgtct cagtaccaag tcgctgagca tcttcttcgg cttggtgctg
     1681 ctcgcggccg ggatccgcat gatcttcagc accgacccgc tcccagtggc gagtggattc
     1741 gacgacttga tgctggggct catcgccgtg ggcattgggc tgctctccgg gtttctggcc
     1801 ggcgtggccg gaatcggtgg tggcatcgtc atcgttccgg caacagtgct gttgctcggc
     1861 ttcacccagc acgaggcgca aggaacgtcg ctgctggcca tcatcctcac ctcggtcgcc
     1921 gcgacgatca tcaatcgaaa gaaccggcgc gtacggttga gggaggcggt cttggtcgga
     1981 gccggcggga tggtcggtgc ggttctggcc tcggtggccg ctctcggggt cgacggccgc
     2041 gtcctgtctg cgctgttcgg ggcattcgca gtcctggtcg ccgttcgcac tctgtatcag
     2101 gcctacacca cagagccgca accggtctag tcgatggtca ccccgtgccg gcgcatctcc
     2161 tcgatcgctc tttgcccgtc gccgggctcg aggtccaccg aggcagtggc gtccggtatc
     2221 acgaacgtgt cgtagtcctt cgcggcgtct agggcggtct ccttgacgca ccagtcgaga
     2281 gccagaccga caaccaccac ccgctggcag ccccgctcat cgagcatccc tcgcaaagct
     2341 gtcggagtct ccttaccggt gactggatcc cgcatcgtga accccgagta gccgtcctcg
     2401 ccgtttgcgc cgttcttgac aaccggcccc gagacctcga gaccgggatg gaactccgcc
     2461 ccccaggtgc cctggacgca gtgcaccggc cagatccccc cgtccttctc gaaatgcggg
     2521 gtggactctg ggtgccagtc ctgggtgtag accacgaacg caccggcgga gagcgcccgc
     2581 atcacctcga tgttgactcg cgggacgaca gcgtcgccac cctggacgaa aaggctgcca
     2641 ttcgggtcgg cgaagtcgtt ctggacgtcg accacgacga gagcggtgct ggtgacatag
     2701 ctgatcacgc tcggcaggct acaagcgagc aataggcttg ggccatggga atctcccggg
     2761 gcgccctact caccgatcag taccagttga cgatgtctca gctgtacttc agacacgggc
     2821 tccacgaacg cgacgcccgc ttcgagcatt tcttccggcg ctatcccgac tacggcgacc
     2881 atgaggcggg atacgccatc aatgccgggt ggggctggct ggggcgatgg gccgaccaag
     2941 cccgctttgg caccgaggag cgtgatgcgc ttcggtctca ccaagtcgag ggccatcgca
     3001 tcttctccga cgatttcatc gagtggttgg gcggcgtgag ctgggattcg ttgcgctacg
     3061 aggccatccc cgaagggagg gtggcccacc ccaacgtgcc gatgacggtc gttgaagggc
     3121 cgctcgccgt ggcccagatc ttggagacgt cgctcctcaa tcatctcaac tttcacacgc
     3181 tcgtcgccac caaagcatcg agagtggtcg aggcgacaag gggcggcgcc gtcctcgagt
     3241 tcggcctgcg ccgggcgcct gacgaaggag gcatcgcggc gaccagagcg gccctgatcg
     3301 ggggagcgaa cgcatcttcc aacgtcggaa tctcccacga gatgggggtc atgccgcagg
     3361 ggactcatgc tcattcgatg gtgcaggtct tcatggcgct aggtgacggt gagctcgggg
     3421 ccttccgcgc ctatgccgat atgtatccgg acgaatgcgt cctgctcgtc gacacaattg
     3481 acaccctggc gtcggggatc ccgaatgcga tcactgtctt cgaggagctg cgagtcagag
     3541 gtcatagacc gatcggtatc cgtctcgact cgggtgatct cgcacatctg tcggtgttgg
     3601 catcgcaaat gttggatcgg gccggtttcg aagacgtgcg cattgtcctg tcgagcggtc
     3661 tcgacgagat gaccatcttc cagatcctca cccagatcgc agatgaagct ccgcgctacg
     3721 gagccgatcc cgaccgagtg atccgacgtc tcctctacgg agtggggacc aagatggcca
     3781 cctcggacgg ccggccctac ctcgacggcg tctacaagct ggtcgccatc gccgacgagg
     3841 agggatggcg accggcgatc aaggtgtcgg acactccttc caagatcatg aatccgggcc
     3901 gcaaacgggt ccatcgtctc tacgaccaac gcgggctcgc caccgtggac ctcatggcgc
     3961 tggccgacga gaacctcaaa ttcccgctcg aagtgcgtca tcatcgcgag accgggatcg
     4021 gccgcaccct cgacgaatcg agcgtctcgg cgaccgagga gctactcgag ccgatgaccc
     4081 tcgcggcacc ctccgatgac ccgtccgttg tggccgaggc gagaaagcgc cgggtgtcgg
     4141 acctcgagcg cctcgacccc ggggtcaagc gtctggtcaa ccctcacgtt tatcacgtgt
     4201 cggtgaccag acgccttgcc gatctcaaga cccggctgat cgaggagacg ctctagggga
     4261 ggtcgtcgct cgcctcggac tgcgagacga gggcaccggg ctcatccttg tcatccctcg
     4321 gcctcaggaa caccggcacc aggatggcca acccgatggc gaggaggaga accggccaga
     4381 accaggctcc gagcgaagtc accgcgtcga tca
//
LOCUS       DASTXO010000505         2164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_657430, whole genome shotgun sequence.
ACCESSION   DASTXO010000505 DASTXO010000000
VERSION     DASTXO010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657430"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VFU96_10750"
     CDS             <1..498
                     /locus_tag="VFU96_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113787.1"
                     /translation="QAGGARTNAAQQRTAANQFRLADLSVFENYIDAVILERTEVADF
                     YLQRFRDEFRPAFDAWMALDPLNNPDAPPSPFAMPEYQLSQEAEGDRLEARADELFAA
                     GEDANSISDVYTLTTLLFAAVLFFAAMAERFEYFKAQVALLIIAGIGLVIGLGIALDQ
                     PITSG"
     gene            complement(514..1485)
                     /locus_tag="VFU96_10755"
     CDS             complement(514..1485)
                     /locus_tag="VFU96_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEU5113788.1"
                     /translation="MKAIVREEYGSPDVLEIGEIDKPVPRDDEVVVKVHAASVNTADL
                     DYLMGRPRITRVASGLRRPRNGRIGLDVAGRVEAIGRDVSTPRIGDDVWADLFDFGHG
                     AFAEYVCGPGSAFTPMPNGVTYEQAATVPHSGVLALQGLLGKGEINPGEKVLINGAGG
                     CVGPFAIQLAKSFGAEVTGVDHGGKFALMHLAGADHVIDYTIEDFTKNERRYDLILDI
                     VDEHSVLHYRRSLLPGGRYVLIARKLTGFAQAFLVGAGVSVVGSKRMGIFVWKANQRE
                     DLEVIKGLIENRKVTPIIDRSFGLGDVADALRYLEDGHARGKIVITI"
     gene            complement(1504..>2164)
                     /locus_tag="VFU96_10760"
     CDS             complement(1504..>2164)
                     /locus_tag="VFU96_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEU5113789.1"
                     /translation="AAARENKLALKPTNLSFEEAAVIPISGMTALQALRDKGQVRPGD
                     RVLIIGAGGGVGTFAVQLAKAFGADVTGVCSSSKVDLVLSIGADHVIDYTLEGFADGA
                     RQYDLIIDNAGNRPLSQLRGALAAEGTLVIVGGEGGGAWVGPADRIVPVAILSPFVRH
                     RLLSLMAAERQDDLELLAEMAESGKFTPAVDRRYPLVEAPDAVRYVGEGHARGKVVVT
                     I"
BASE COUNT          384 a          740 c          629 g          411 t
ORIGIN      
        1 caggccggcg gcgcacgcac caatgcagcc cagcaacgga ctgcggccaa ccaattccgc
       61 ttggccgacc tgagcgtgtt cgagaactac atcgacgctg tcatcttgga gcgaactgag
      121 gtggccgatt tctatctcca acgatttcgt gacgagttca ggccggcctt cgacgcgtgg
      181 atggctctcg acccgctcaa caaccccgac gctccgccaa gcccgttcgc catgcccgag
      241 taccaactat cccaggaggc cgagggcgac cgtctcgagg cccgagccga tgagctcttc
      301 gccgccggag aagatgcgaa cagcatctcc gacgtctaca cattgaccac cctcctcttc
      361 gccgccgtcc tcttcttcgc cgcgatggcc gagcggttcg aatacttcaa agcccaggta
      421 gccctcctca tcatcgccgg gatcggtctc gtcatcggcc tcggaatcgc tttggatcag
      481 ccgatcacca gcggctaggc atcctcaaag ctgtcaaatg gtgatgacga tcttgccccg
      541 ggcgtggccg tcttccagat agcggagcgc gtcggcaaca tcccctaacc cgaagcttcg
      601 gtcgatgatc ggggttactt ttcgattctc gatcagcccc ttgatgactt cgagatcttc
      661 tcgctggttc gccttccata cgaagatgcc catccgcttg ctcccgacga cggagactcc
      721 agcgccgacc aggaaggcct gagcgaagcc cgtcagtttc cgggcgatga ggacgtagcg
      781 ccctcccggg aggagggacc gccggtagtg caggaccgag tgctcgtcga cgatgtcgag
      841 aatcaggtca tatcgtcgct cgttcttcgt gaaatcctcg atggtgtagt cgatgacgtg
      901 gtccgcgccc gccaggtgca tcagggcgaa cttccctccg tggtcgacac cggtaacttc
      961 ggcgccgaac gatttggcga gctggatcgc aaagggcccg acacagcctc ccgctccatt
     1021 gatcaggacc ttctctccgg ggttgatctc tcccttgcca agcaggccct gcagcgcgag
     1081 gacgccggag tgaggaaccg ttgccgcctg ctcgtatgtc acgccgtttg gcatcggcgt
     1141 gaacgcactc cccggcccgc agacgtactc ggcgaaggcg ccatggccga agtcgaacaa
     1201 gtccgcccac acatcatccc cgatccgggg tgtgctcaca tctcgaccaa ttgcctcgac
     1261 tcggccggcg acatcgagac cgatgcggcc gtttcggggt ctgcgcagcc cggacgccac
     1321 gcgagttatc cgcggcctgc ccatcaagta gtcgaggtcc gcggtgttca cggacgcggc
     1381 gtgaaccttc accaccacct cgtcgtccct cggcacgggc ttgtcgatct cccctatttc
     1441 gaggacgtcg ggtgagccgt actcctcgcg gacgattgcc ttcacgacgt gcccctcgat
     1501 cggtcagatg gtgacgacga ccttgcctcg ggcatgcccc tctccgacat atcggaccgc
     1561 gtcgggcgct tcgaccaacg ggtatctccg gtcaacggcc ggtgtgaact tgcccgattc
     1621 tgccatttca gcgagtagct ctagatcgtc ctgacgctcg gccgccatca gcgacaagag
     1681 cctatgtcgg acgaacgggg acagtatcgc cacgggcacg atgcggtctg ctggcccgac
     1741 ccaagctcct cctccctcgc cgccgacgat gacgagggtc ccttctgccg ccagggcgcc
     1801 tcggagctgg gacagcgggc ggttgcccgc gttgtcgatg atgaggtcgt actgacgtgc
     1861 cccgtccgca aaaccctcga gtgtgtaatc gattacgtga tcggccccga tcgacaggac
     1921 gagatccact ttggacgagc tgcacacccc ggtgacgtcg gccccaaacg ccttggcgag
     1981 ttggactgcg aaagtgccaa cgccgccccc ggcgccgatg atcagcaccc tgtcacctgg
     2041 tctcacctgg cccttgtcac gcagggcctg aagcgcggtc atgcccgaga tcggaatgac
     2101 cgcagcctct tcgaagctga gattcgtcgg cttgagcgcc agcttgttct cccgggcagc
     2161 cgca
//
LOCUS       DASTXO010000506         3864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_952208, whole genome shotgun sequence.
ACCESSION   DASTXO010000506 DASTXO010000000
VERSION     DASTXO010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952208"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1026)
                     /locus_tag="VFU96_10765"
     CDS             complement(79..1026)
                     /locus_tag="VFU96_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007542675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5113790.1"
                     /translation="MWFHAVRTEEPVRAETPPSVVRESPGRVKLRRFLASTREVFSRG
                     STRFGGVLIVLMLGMAIFAPLIVDENPRGAYQMPRDLSAVAAPPGTEGHPLGTTRLGG
                     DVLYGVVWGARLSLLLSIYVVAGALIIGLIVGSTAGIIGGKVDAFMMRVVDVFLSIPT
                     LVFPLAIAAILGPSFINIVVALAVIMWARYARIIRSQILSVREEEYVEAARAIGDSRW
                     GIIRKDILPNSFSPVAVQATLDMGHAVLLGATLAFIGLAVAGQAEWGTLVSEGQADIV
                     AGRWWTSTFPGLMVFLWALSFNLVGDGLRDVLDPRTEGR"
     gene            complement(1031..2041)
                     /locus_tag="VFU96_10770"
     CDS             complement(1031..2041)
                     /locus_tag="VFU96_10770"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5113791.1"
                     /translation="MHKYIGRRLLALPFVILAVSILVFGLTRLGGTTIGPYLTPGMTD
                     EEIAVLEERFNLDEPVPMQYLAWLGGVVQGDLGWSAVSVAPVIEVFREKIPASFELGA
                     AAALWAVTLGIGLGTFAARRRNRVHDQLTSVVAVGGASMPTFWLGIVLLIIFWSYLGW
                     FPHGRSDPEIWNSIPHPTNFYTIDAILAGSFAGFKDAIWHLVLPALTLGYAAVGIVTR
                     MMRSSLLEEMGRDYVDAARARGLPENLVVRRHARRNAFIPTITVIGLSIGIMFEGTVV
                     VETIFGWPGLGSWMASSVLAGDQATTLAFVIFTSILFLTINLIVDVMYGYLDRRVTLR
                     KA"
     gene            complement(2139..>3864)
                     /locus_tag="VFU96_10775"
     CDS             complement(2139..>3864)
                     /locus_tag="VFU96_10775"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5113792.1"
                     /translation="SRRTPMGRKSIAWRMLALVAILALVIAACSAGETTDDTTDGTTD
                     GTTGTSAASGEVPNPDVLVATVNDDVFNLDPAAVDDNDVGMNVILNVYDRLVALAPED
                     GAVVPSLATEVPTVENGLISEDGLTYTFPLREGVVFSDGSELTAEDVKYSWDRVVEMG
                     LPEGQAQLFDNVAETTVVDDHTFQVTLNQVDASFLAYLVAHSVASVVSQDYVEANGGI
                     TAGQPNEHMAANMAGSGPYTLTNWERADRMEFDINPDYWGEPAHLPVRWELIVDSPAT
                     GLQAREYDIVDISPTDVPSFEGIDHAVLNTEVLDLQVLQIGMNMNIAEGALPDGDTIP
                     PDFFQDVRVRQAFNYAFDYQGYIDGILGGLGGRNSFILPVEMYGFDPAAPIYETDPAM
                     AEQLFTEAGYWDEGFTVSIYTDQTHEGFTGAALVLKDSIEALNPNFHINVLQVPEAQF
                     DEVLATDPLPTAMWSYTSSQYSTPDAYFFDSALSDGRYGAVNSFADGYTDAAAIDDMI
                     NSARTALDEETRLGIISDLQTALYEEAMWIMPANEGAPTAAGDWVTGHIENPMWHRPS
                     QYWAIYDK"
BASE COUNT          726 a         1220 c         1273 g          645 t
ORIGIN      
        1 cgaggctgga tcgggcccga ggaccggaca ctcgttttca acaccggaac cggcctcaaa
       61 tatcgctgag atcttgggct agcgcccctc ggtgcggggg tcgaggacgt ctcgcaagcc
      121 gtcacctacg aggttgaacg acagggccca caggaacacc atgaggccgg ggaacgtgga
      181 ggtccaccaa cgcccggcga cgatgtcggc ctggccctcg ctgaccaggg ttccccactc
      241 cgcctggccg gcgacggcca ggccgatgaa agcgagggtg gccccgagca gaactgcgtg
      301 acccatgtcc agggtggcct gcaccgccac cggcgagaat gagttgggca ggatgtcctt
      361 gcggatgatc ccccatctcg agtcgccgat ggcacgggcg gcctcgacgt actcctcttc
      421 tcgcaccgag aggatttgac tccggatgat ccgggcatag cgcgcccaca tgatcacggc
      481 cagggcgacc acgatgttga tgaaggacgg ccccaggatg gcggcgattg cgagtgggaa
      541 caccagggtt gggatggaga ggaagacgtc gacgacacgc atcatgaagg cgtcgacctt
      601 gccgccgatg atgcccgccg tgctccccac gatcagaccg atgatcagcg ccccggccac
      661 gacgtagatc gagagcagca gggagagacg ggccccccac accaccccat agagaacgtc
      721 tcctcccaga cgcgtcgtac cgaggggatg accttcggta cccggtgggg cggcgaccgc
      781 actgagatcc cttggcatct ggtaagcgcc gcgcgggttc tcgtccacga tcaacggggc
      841 gaagatcgcc atgcccaaca tcaacacgat caagacgccc ccgaaccggg tgctaccccg
      901 gctgaagacc tccctggtgc tggccaggaa ccgtcggagc ttgacccggc ccggagactc
      961 ccggaccacg gaggggggtg tctcggcccg gaccggctcc tcggtgcgaa cggcgtggaa
     1021 ccacatgggt ctaggccttc ctcagggtga cgcggcggtc gaggtacccg tacatcacgt
     1081 cgacgatcag gttgatggtg aggaagagga tgctggtgaa gatgacaaag gcgagggttg
     1141 tcgcctgatc acctgcgagc accgagctcg ccatccagct accgaggccg ggccagccga
     1201 agatcgtctc cacgaccacg gtgccctcga acatgatgcc gatggaaagg ccgatgacgg
     1261 tgatggtcgg gatgaaggcg ttgcgtcgag cgtgacggcg gacgacgagg ttctccggga
     1321 ggccgcgagc ccgggcggcg tcgacatagt cccgccccat ctcctcgagc agcgaggagc
     1381 gcatcatcct ggtgacgatg ccgacggcgg cataaccgag agtgagagcg ggcagaacga
     1441 gatgccagat ggcgtctttg aagcctgcga aggacccggc caggatcgcg tcgatggtgt
     1501 agaagttggt cgggtggggg atgctgttcc agatctcggg atcggatctc ccgtgcggga
     1561 accagcccag gtaagaccag aagatgatga ggagaacgat cccgagccag aacgtgggca
     1621 tgctggcgcc gcccaccgcc accacgctgg tgagctgatc atgtacccga ttgcggcgtc
     1681 gggccgcgaa cgttcccaac ccgattccca aggtgactgc ccacaaagcg gccgcagccc
     1741 ccagctcgaa cgaggccggg attttctcgc gaaagacttc gatgaccggg gccacggaca
     1801 cggccgacca gccgaggtcc ccttggacca cccccccgag ccaggccagg tattgcatgg
     1861 ggaccggctc gtcgaggttg aaccgttcct cgaggacggc gatctcctca tcggtcatcc
     1921 cgggcgtgag atatggcccg atggtggtgc cgccgagacg ggtgagacca aagaccagga
     1981 tgctcaccgc caggatgacg aagggcagtg ccagcagccg gcgaccgatg tacttatgca
     2041 accttcagat ctcctcgccc gagtctacgg cacctccgga ccagggatgg agggccgccg
     2101 gaggggcagg tgctccgccg gcggcctcga atccatgctc acttgtcgta gatcgcccag
     2161 tactgggacg gccgatgcca catcgggttc tcgatgtgac cggtcaccca gtccccggcc
     2221 gccgttggtg cgccctcgtt ggcgggcatg atccacatcg cctcttcgta gagggcagtc
     2281 tgcaggtctg agatgatgcc gagacgggtc tcctcgtcga gcgcggtcct cgccgagttg
     2341 atcatgtcgt cgatggcggc cgcgtcggtg taaccgtcag cgaagctgtt gaccgcgcca
     2401 tagcgcccgt ccgacaacgc actgtcgaag aaataggcgt cgggcgtgct gtactgggag
     2461 ctggtgtagg accacatcgc cgtcgggagc gggtcggtcg ccagcacctc gtcgaactgc
     2521 gcctcgggca cctgcaggac attgatgtgg aagttggggt tgagagcctc gatactgtcc
     2581 ttcaggacga gcgccgcccc ggtgaagccc tcatgggtct ggtcggtata gatcgagacc
     2641 gtgaatccct cgtcccagta gcccgcctcc gtgaacagct gctcggccat cgccggatcg
     2701 gtctcgtaga tcggggcagc tggatcgaac ccgtacatct cgaccgggag gatgaagctg
     2761 ttccgccccc ccagtccgcc caggatccca tcgatgtagc cctgatagtc gaaggcgtag
     2821 ttgaacgcct ggcgcacccg gacgtcctgg aagaagtcgg gcgggatcgt gtcaccgtcg
     2881 ggcaaggcgc cctcggcgat gttcatattc atcccgatct ggagcacctg gaggtcgagg
     2941 acctcagtgt tcagcacggc atgatcgatc ccctcgaagg aaggcacgtc ggtggggctg
     3001 atgtcgacga tgtcgtactc tctcgcctgc agacccgtgg ccggcgagtc gacgatcagc
     3061 tcccagcgca ccggcaggtg agccggctca ccccagtagt cggggttgat gtcgaactcc
     3121 atccggtcgg ctcgctccca gttggtcagg gtatatgggc cggagcccgc catgttcgcc
     3181 gccatgtgct cgttgggctg gccggcggtg atgccaccat tggcctcgac atagtcctgg
     3241 ctcaccacac tggccaccga gtgggcgacg aggtaggcga ggaacgaggc gtcgacctgg
     3301 ttcagcgtca cctggaatgt gtgatcgtca actaccgtcg tctcagcgac gttgtcgaaa
     3361 agctgggcct ggccctctgg caagcccatc tcgaccaccc ggtcccagga gtacttcacg
     3421 tcctcggcgg tcagctcgct gccatcggag aagacgactc cctcacggag cgggaaggtg
     3481 taagtaagac cgtcctctga gatcagaccg ttctcgacgg tcgggacctc cgtggccagc
     3541 gatggaacca cggcaccatc ttcaggagcg agagcgacga gccggtcgta cacgttcagg
     3601 atcacgttca tgccgacgtc gttgtcatcg accgcggccg ggtcgaggtt gaacacgtcg
     3661 tcgttgacgg tggcgaccag cacatcgggg ttgggcacct ccccactggc cgcggatgtg
     3721 cctgtcgtac catcagtcgt accgtcagtg gtgtcgtcgg tcgtctcccc ggcgctacag
     3781 gccgcgatga ccagcgcgag gatggccaca agggctagca tcctccacgc aatcgattta
     3841 cgtcccatgg gagtcctcct cgat
//
LOCUS       DASTXO010000507         2471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_620926, whole genome shotgun sequence.
ACCESSION   DASTXO010000507 DASTXO010000000
VERSION     DASTXO010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620926"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1627)
                     /locus_tag="VFU96_10780"
     CDS             complement(<1..1627)
                     /locus_tag="VFU96_10780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEU5113793.1"
                     /translation="MSEGTPEYPVRVDRRVFVPMDDGVIIALTVYQPDSDNDGPYPTI
                     VESLPYRKDDAFYSSDWPTYAYLARRGFAGVRIDIRGTGASTGTIEDEYVPREQEDTL
                     AVFRWLEDQEWCTGKLGMWGVSWGGFSSLQTAMLRPPQLRAIAPVHATHDRFACDVHY
                     TGGSLHAQEQVDWPPSMVVCNGLPPDPDIVGEVWYEQWMERLEKTPQWPGIWLRHQRR
                     DEYWLHGSPCADYAAIQCPTLLIGGWVDGYVDGMLAMAEHLTCPTRTVIGPWGHYRPA
                     TGRPGPTLDHLDLLARWFGHHLRGDDNGVMDMPTVTAFIRTGTPYDGPTHPPFDDPPT
                     PGYWRAEPAWPPTDRVVVDLSLSDLQHDATTWSGPQWVGVHAPAWDRAGRGSADSADD
                     DAHSLTFETAPLDEQLEILGTPEIEVTVASDKTVGLIAARLLAVSPTGEGHLITRGSR
                     NLAFPDDLSTPQPIEPDTPIKVDFPLMTTSAVLPAGWRLRLALSGADFPVVWPPGESF
                     TLSLDLNGSNIHLPTVPARGDESRISITVPNPLP"
     gene            1643..>2471
                     /locus_tag="VFU96_10785"
     CDS             1643..>2471
                     /locus_tag="VFU96_10785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5113794.1"
                     /translation="MARHTLAAVMNDNTPRQVIVVGAGVAGLVVAKRLSDRGLDVLVL
                     EARDRVGGRLWSHHLPNGEIVELGGEWISTSQGAVVGLANELGLELIETGMDFISRDP
                     IGGAPIAAAEHERLARLLHDRMADIGPPGLSSISAQEVLDRLGEAGPAMSILRSRLQG
                     TAGAPLESIAAAEIGEEFGIGDQASYVRVDGGNDRLAIALAPDLDVRLGIEVTSIRQT
                     SQTVAVATANEDFTGLALVLAVPLGVLKRLRFDPELPDEVASAIGRMGMGVGIKVAIG
                     "
BASE COUNT          411 a          777 c          843 g          440 t
ORIGIN      
        1 gcgggagagg atttgggacc gtgatcgaga tcctggattc atcaccacgc gccgggacag
       61 tcggcaaatg gatattggag ccgttcaggt cgagggacag cgtgaaactc tctccgggcg
      121 gccagaccac gggaaagtcg gcaccagaca gggcgagccg caaacgccag cctgcgggca
      181 ggacggcaga cgtcgtcatc aacgggaaat cgaccttgat cggagtgtcc ggctcgatcg
      241 gctgtggtgt ggataggtca tccgggaatg ccaggttccg gctgcccctg gtgatcagat
      301 gcccctcgcc cgtcggcgag actgcgagca gcctggccgc gatcagcccc accgtcttgt
      361 cactggccac cgtgacctcg atctcgggcg tccccaggat ctccagctgt tcgtccagtg
      421 gtgcggtctc gaaggtgagc gagtgggcat cgtcgtccgc cgaatcggcg ctgccccgcc
      481 cggctcgatc ccaagccggg gcgtgcaccc cgacccactg cggcccggac caggtcgtgg
      541 cgtcgtgttg gagatcgctc aagctcagat cgacgacgac gcggtcggtg ggtggccagg
      601 caggctcggc tcgccagtat cccggcgtcg gcgggtcgtc gaaaggtggg tgggtcggcc
      661 cgtcgtatgg ggtgccggtg cggatgaagg cggtcaccgt gggcatgtcc atgactccgt
      721 tgtcgtcacc cctgaggtga tgcccaaacc atcgagcgag gaggtcgaga tgatcgagcg
      781 tcggcccagg ccgcccggta gccggccggt aatggcccca gggcccgatg acggtcctcg
      841 tggggcaggt gagatgctcg gccatggcca gcatcccgtc gacatacccg tcgacccagc
      901 caccaatgag cagtgtgggg cattgaatgg ccgcgtagtc ggcgcatgga gagccgtgga
      961 gccagtactc gtcgcgacgt tgatggcgga gccaaatccc gggccattga ggggtcttct
     1021 ccagccgttc catccattgc tcgtaccaaa cctccccgac gatgtccggg tcggggggaa
     1081 ggccgttgca gaccaccatc gacgggggcc aatccacctg ctcctgggca tggagcgagc
     1141 caccggtgta gtgcacatcg caggcgaagc ggtcatgggt cgcgtgaacc ggggcgatcg
     1201 cccttagctg gggcggccgc agcatcgcgg tctgtaggga gctgaacccg ccccaggaca
     1261 caccccacat cccgagcttt ccggtgcacc attcctggtc ttccagccag cggaagaccg
     1321 ccagcgtgtc ctcctgctcc ctcggcacgt attcgtcctc gatggtgccg gtcgaggccc
     1381 cggttcccct gatgtcgatg cgaactccgg cgaatcctct ccgggccagg tatgcatacg
     1441 tgggccaatc gctcgagtag aaggcgtcgt ccttgcgata ggggagcgat tcgacgatcg
     1501 tcggataggg cccgtcgttg tcgctgtcgg gctgatagac cgtgagggcg atgatgacgc
     1561 catcgtccat cgggacgaac acccggcggt cgactcggac cggatactcg ggtgttccct
     1621 cagacacggc ggcaatctag ccatggcccg gcatacgctc gctgcggtca tgaatgacaa
     1681 cacaccccgt caggtgatcg tggtcggcgc cggagtggcg ggtctggtgg tagcgaaacg
     1741 tctatccgat cgagggctcg acgtcctggt tctggaggct cgggaccggg tcggaggccg
     1801 tctctggtcc catcatttgc ccaatggcga gatcgtcgag ctgggcggcg agtggatctc
     1861 gacttcgcag ggggcggtag tcggccttgc caacgagctc gggctcgagc tcatcgagac
     1921 cggcatggac ttcatctcgc gcgacccgat tggtggcgct ccgatcgcag cggcagagca
     1981 cgagcgtctc gcccggctcc tccacgatcg gatggcggac atcggtccgc ccgggctgtc
     2041 atcgatcagc gctcaggagg tcctcgaccg cctgggtgaa gcgggtccgg cgatgtcgat
     2101 tctgcgttca cgtctccaag gaaccgccgg agccccgctg gagtcgatcg ccgctgccga
     2161 gatcggtgag gagttcggca tcggtgacca ggcgtcgtac gttcgtgtcg acggtggcaa
     2221 cgaccgcctc gccatcgccc tcgcccctga cctcgatgtc cgcctcggaa tcgaggtcac
     2281 ctcgatccgt cagacgtcgc agaccgtcgc ggttgcaacc gccaatgagg atttcaccgg
     2341 cttggctctc gtgctcgctg tcccacttgg agtcctgaaa cgcttgagat tcgacccgga
     2401 actgcccgac gaagtggcct cggcgatcgg gagaatgggg atgggtgtgg gtatcaaggt
     2461 cgcgatcggc a
//
LOCUS       DASTXO010000508         3226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1504720, whole genome shotgun sequence.
ACCESSION   DASTXO010000508 DASTXO010000000
VERSION     DASTXO010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504720"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /locus_tag="VFU96_10790"
     CDS             <1..1265
                     /locus_tag="VFU96_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113795.1"
                     /translation="HALAGPLRPTTKDPLVLSSERRADDYRHIGGVVCDVVTGRAPIL
                     EELILVDTPDIDSTSVEHRAMAETMIDNADIVVYVSSALRYADLVPWEVLRRAHSRSV
                     PVIQVMNRIRGSSRGALADYASRLEAEGLGSDVVAVQEHHIGPGAQTIPPAAVQELRD
                     RLVSVVEARRAGAADVVRSVFDTTVEQTRQVIEEAMVLLDETGITTTHVRQLLDVDLG
                     RIGVRRENVRGGGLDLRPLADLGLRRLRTKGMIRRRLPASADVDRSLALFDESLIAAV
                     DADMRRQLHAGSLINGEERKKLLSDTHLAARTAIARWRTEVGDLPPVMASLDPPLISL
                     ILGGCCFEAPDRGIEEVVTLLAPGAQLDELSSDVKSRLAESLVPVYAGAEHRIVSRMS
                     AAAMTRRSIDRVRASRWAVIARSSFANA"
     gene            1258..2478
                     /locus_tag="VFU96_10795"
     CDS             1258..2478
                     /locus_tag="VFU96_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113796.1"
                     /translation="MHDALVGLLDKVDTAVAESAGALRRTEVDSVAALARATRMRLSY
                     PESIVVVALAGGTGSGKSSLVNAIVDEDVALTGGIRPMTSAPLALVPTDGAEALGGYL
                     DELGISDRVEHHGPSWLCVLDLPDNDSVEVEHRHRVDSLLPRVDMIVWVTDPEKYRDA
                     VIHHRYISPMSGYQEQFIFVLNQIDRLDPEDAVVVADDLGRALREDGIREPKVIATAA
                     SPVAGPPFGVDLLMDALGAAVGRRLAVNRKALADLANASSQLVRSSSTAHAVDFERQW
                     AEEVASAVDLAIAGRAAGGGHDLAEYVTRLATEMGGETQHRLQDLAIETHGAFLRCVA
                     AEGSTTGTSRSWLRRRRRDAGPAGTANREDLIARVDVEIGGPIRELLVQRGRAHAAIT
                     DLALALGDLERTSG"
     gene            2494..>3226
                     /locus_tag="VFU96_10800"
     CDS             2494..>3226
                     /locus_tag="VFU96_10800"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEU5113797.1"
                     /translation="MDATAANPWLATAIVVGSGVVLYLILAGIGARFVTRVEERTPGS
                     AARVLTLWVMVRRVTLIVILVLVVLMLIDIWGLNMAPFLAVGTAVAAALGFGAQNLIR
                     DVIAGFFILAEDQYRIGDTVTVAATTGKVEDIQFRVTVLRDGEGNVHYVPNGQITVTS
                     NLTSLYASPSIDVRVGYQADVDRAMDVMLDELMRLSAEAPWSDMITKEPQMLGVQDLV
                     GSGVFLRARLTTAAEQRWSVQREALR"
BASE COUNT          527 a         1044 c         1091 g          564 t
ORIGIN      
        1 cgcatgcgct ggccggcccg cttcgaccga ccaccaagga tccattggtc ctctcctccg
       61 agaggagggc ggacgactat cggcacatcg gtggtgtcgt ctgcgacgtc gtgactggcc
      121 gcgcccccat cctggaggag ctgatcctcg tagacactcc ggacatcgac tccacatcgg
      181 tcgagcatcg ggccatggcc gagacgatga tcgacaacgc cgacatcgtc gtgtacgtca
      241 gctcggctct ccgctatgcc gatctggtgc cgtgggaagt gctgcgccgg gctcactcga
      301 gaagcgttcc tgtgatccag gtcatgaacc gcattcgggg ctcgtcacgg ggtgcgctcg
      361 ccgactatgc gtcgagactc gaggccgaag ggctcgggtc ggacgtagtg gccgtccagg
      421 agcaccacat cggcccgggg gcccagacca tcccgccggc ggcggtccag gagctgcgag
      481 atcggctggt atcggtagtc gaagcacggc gggccggcgc agcggacgtg gtccggtcgg
      541 tcttcgacac cacggtcgag cagacgcgtc aggtgatcga ggaggccatg gtcttgctcg
      601 acgagaccgg cataacgacc acccatgtgc ggcaattgct cgacgtagat ctgggacgga
      661 tcggtgtccg tcgtgagaac gtgaggggag gcggcctcga tctccgcccc ctggccgacc
      721 tcggcctccg ccgtcttcgc accaagggaa tgatccgtcg ccgcctgccc gcgtcggccg
      781 atgtcgaccg cagcctggca ctgttcgacg aatcgctcat agccgcggtc gatgccgaca
      841 tgcggcgtca gctccacgct ggaagcctga tcaacggcga ggagcgcaag aaactcctct
      901 ccgacaccca cctcgccgct cgaacggcaa tcgcgcggtg gaggaccgag gtcggggacc
      961 tgccaccggt gatggcgtcg ctcgatcctc ctttgatatc cctcatcctc ggtgggtgct
     1021 gcttcgaagc gccggaccgg ggcatcgagg aggtagtgac gctcctggct ccgggggccc
     1081 aactggacga attgtcgtcc gacgtcaaga gccgcctggc cgagagcctc gttcccgtgt
     1141 acgccggcgc tgagcaccgc atcgtctccc ggatgtcggc agcagcgatg acccggcgct
     1201 cgatagatcg tgtcagggca tcgaggtggg ccgtcatcgc gaggtcgtct ttcgccaatg
     1261 catgatgcgc ttgttggcct tctcgacaag gtcgacacgg ctgtggcgga gtcggccggg
     1321 gctctacgtc gaaccgaggt cgactcggtc gccgccctgg cccgtgcgac cagaatgagg
     1381 ttgtcgtatc ccgagtccat cgtggtggtc gcgctggcgg gaggcacagg gtcgggaaaa
     1441 tcgagtctcg tcaacgcgat agtcgacgag gacgtcgccc tgaccggcgg catccgcccc
     1501 atgacctccg caccgctcgc actggtgccc acagatggcg ccgaggcgct cggtgggtat
     1561 ctggacgagc tcggcatctc cgatcgtgtc gagcatcacg gcccgtcctg gctgtgtgtc
     1621 ctcgatctcc ccgacaacga cagcgtggag gtcgagcatc gtcatcgggt ggactcgcta
     1681 ctgccgcggg tggacatgat cgtctgggtc accgaccccg agaaataccg ggacgcagtc
     1741 atccatcatc ggtacatctc gccgatgtcc ggctaccagg agcagttcat cttcgtcctg
     1801 aaccagatcg accggctgga cccggaggac gccgtcgtcg tggccgacga cctgggcagg
     1861 gcactccgcg aggatggcat ccgcgagcca aaggtgatcg ccaccgcggc cagcccggtg
     1921 gcagggcccc cttttggagt ggacctgctg atggacgcgc tgggggccgc cgtgggccga
     1981 cgtttggcgg tgaatcgcaa ggcgcttgcc gatctcgcga atgcgtcctc acagctggtc
     2041 aggtcctcgt cgaccgccca cgccgtcgac ttcgagaggc aatgggccga ggaggtggcc
     2101 tccgccgtcg acctggccat cgccggtcgg gcggcaggcg gtggacacga tctcgccgag
     2161 tacgtcaccc gcctcgccac cgagatgggc ggcgagaccc aacaccgttt gcaagatctc
     2221 gcaatcgaga cccacggcgc gtttctcaga tgcgtcgccg ccgagggctc gaccacaggg
     2281 acgtcgcggt cctggctgcg acgtcgacgc cgagacgccg gtcccgccgg caccgcgaac
     2341 cgcgaggatc tgatagccag ggtcgatgtc gagatcgggg ggccgatccg cgagctcctg
     2401 gtgcagaggg gccgggccca tgccgcgatc accgatctcg ccctggcgct gggagatctg
     2461 gagcggacct cgggataact agcctcggcc ggaatggacg cgaccgctgc gaacccctgg
     2521 ctggcgacag cgatcgtggt ggggtccggc gtggtgctct atctgattct cgccggcatc
     2581 ggggccagat tcgtcactcg ggtagaggag aggacgcccg gctccgcagc ccgtgtcctg
     2641 acactctggg tgatggtgcg gcgcgtgacc ctcatcgtca ttctcgtcct ggtggtcctg
     2701 atgttgatcg acatctgggg gctgaacatg gccccgttcc tcgcagtggg aaccgcggtc
     2761 gcggccgcgc tcggctttgg cgcccagaac ctgatcaggg acgtgattgc aggcttcttc
     2821 atcctcgctg aggaccagta ccggatcggt gacaccgtga ccgtcgctgc cactaccggg
     2881 aaggtggagg acattcagtt ccgtgtcact gtcctccgcg atggcgaggg caatgtccat
     2941 tacgtgccca acgggcagat cacggtcacg tccaatctca ccagtctcta tgcctcacct
     3001 tccatcgacg tgagggtggg ctatcaggcg gatgtcgacc gggcgatgga cgtgatgctc
     3061 gacgaattga tgagattgtc ggcggaggcc ccgtggtccg acatgatcac caaggagccg
     3121 cagatgctgg gagtgcagga cctggtcggc tcgggtgtct tccttcgagc ccgactgacc
     3181 acggcggccg aacagcgctg gtcggtccag cgggaggcac tgcgtc
//
LOCUS       DASTXO010000509         2673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_473047, whole genome shotgun sequence.
ACCESSION   DASTXO010000509 DASTXO010000000
VERSION     DASTXO010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473047"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VFU96_10805"
     CDS             complement(<1..241)
                     /locus_tag="VFU96_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEU5113798.1"
                     /translation="MFVPDDFVVPTRFDGPGFQLEPLGPVHNERDHQTWMSSIDHIRA
                     TPGMEEGAWPTPMTLDENMSDLEGHAREFTERDAFA"
     gene            complement(251..712)
                     /locus_tag="VFU96_10810"
     CDS             complement(251..712)
                     /locus_tag="VFU96_10810"
                     /inference="COORDINATES: protein motif:HMM:NF016102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HEU5113799.1"
                     /translation="MRLMFFFTAVALAEMAAFIWVGSIIGFGWALGIAIVTAVLGSIL
                     VRRAGISVWGRFRSKVNQGVLPGRELSDGAAILVSGAFLISPGFITDTLGFLLLIPAV
                     RDLVYRNINKRLSGRVKVLTGDRRTVRADWPGGTEEVLQYEIVDSDIEKRS"
     gene            complement(748..2040)
                     /locus_tag="VFU96_10815"
     CDS             complement(748..2040)
                     /locus_tag="VFU96_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5113800.1"
                     /translation="MSLRDEAAVLAALRGIVGHTHVLTEPDMVAGYVRDWTGRWTGRT
                     AAVVRPGSVTEVEEVVRLCHRTGLSVVPQGGNTGLVGGSVPHRGEVVLSLRRLDRVGP
                     IDPARRSVTVGAGVTVGALQERAREAGLRYGVDLASRDSATIGGTIATNAGGARVVSQ
                     GDTRRQVLGLEAVLADGTHLSGLDGIAKSSVGPDPLQLMVGSEGTLGVITAARVRLLP
                     PETVDSMVTLLAVDRLSDALRFIGPDLRVIEFFDNACLEAVIEHRGLLRPLPGEASFY
                     VLIESGVAPLIPENAAAVVDPRLWAYRESITETINGLGVPVKLDVAVPLERADEFAGA
                     VRSLELEGETYLFGHLAEGNFHVNVVGNPDPDGVVDDVLEIVVGLGGVIASEHGIGVA
                     KATWWRRTSEASMLDFQRSIKEALDPEGILNPAVFWGA"
     gene            2133..>2673
                     /locus_tag="VFU96_10820"
     CDS             2133..>2673
                     /locus_tag="VFU96_10820"
                     /inference="COORDINATES: protein motif:HMM:NF014998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEU5113801.1"
                     /translation="MTEFHYEPLSYLDASFLALETKTSHMHVAGVALLDAAPLKGEDG
                     GIDIERIRAHVESKLRYIPRYRQRLQWVPFDRRPVWVDDEHFAIDYHLRHSSLPRPGT
                     EAQLKQLAGRIVSAPLDRSKPLWELWVVEGLDDDRFALIAKIHHCMIDGISGVDLTTI
                     LLNVVPSAEVEEPEVWVPRP"
BASE COUNT          510 a          919 c          799 g          445 t
ORIGIN      
        1 atgcgaacgc atcccgttcg gtgaattctc gtgcgtgacc ctcgagatcg gacatgttct
       61 catcgagagt catcggcgtg ggccaagcgc cttcttccat gccgggggtg gctcggatgt
      121 gatcgatgct ggacatccag gtctggtgat ctcgctcgtt gtgaaccggt cccaacggtt
      181 cgagctggaa cccgggaccg tcgaagcgcg tcggcactac gaagtcgtcg ggaacgaaca
      241 tggccgctgt ctaggaccgt ttttcgatgt cgctgtcgac gatctcgtac tggaggacct
      301 cctccgtacc cccgggccag tcggcccgaa ccgttctgcg atcgccggtg agcaccttga
      361 cacgtccgga caggcgcttg ttgatattgc gatagaccag atcccgaacg gccgggatca
      421 ggagcagaaa gccgagtgtg tcggtgatga aaccaggcga tatcaggaat gccccggaca
      481 ccaggatggc agccccgtca ctgagctcac ggccggggag cacaccctgg ttcaccttgc
      541 tgcgaaaccg accccacacc gagatcccag cccgtcggac caggatcgac cccagaactg
      601 cggtgacgat cgcgatgccg agcgcccagc cgaacccgat gatggacccg acccagatga
      661 acgcggccat ctcggccaac gccaccgccg tgaagaagaa catcaagcgc atgatccaac
      721 gctaccggcg attggtcaca acgaggctca ggctccccag aacacggctg gattcaagat
      781 cccttcagga tcgagcgctt ctttgatcga tctttgaaag tcgagcatgg atgcctcact
      841 ggttcgccgc caccatgtcg ccttcgccac accgatcccg tgctcgctgg ctatgacccc
      901 gccaagtccg acgactatct cgagcacatc gtcgaccaca ccatcgggat cggggttgcc
      961 gaccacgttc acatgaaagt ttccttcggc gagatgaccg aataggtagg tctcgccttc
     1021 gagttcgagg cttctcacgg caccggcgaa ttcatccgcg cgctcgaggg ggaccgccac
     1081 gtcgagcttg accgggacgc ccagaccatt gatcgtctcg gtgatcgact cccgataggc
     1141 ccaaagcctc ggatccacga cagctgcggc gttctcgggg atgaggggag ctacccccga
     1201 ttcgatgagg acgtagaagg aagcctctcc gggaagcggt ctgaggagcc cacggtgctc
     1261 gatcaccgcc tcgaggcagg cgttgtcgaa gaactcgatg accctgagat cggggccgat
     1321 gaacctgagt gcatcgctca gccgatccac cgcgaggaga gtcaccatcg aatccaccgt
     1381 ctcaggcgga agcagtctca cccgcgccgc cgttatcact cccagggtcc cctcactccc
     1441 gaccatcagt tgaaggggat cggggccgac gctcgatttg gcgatcccgt cgagaccgga
     1501 caaatgggtg ccatcggcca ggaccgcctc cagacccagc acctggcgac gggtgtcacc
     1561 ttgggacacg acacgcgcgc ctccggcatt ggttgcgatc gtcccgccga tggtggccga
     1621 gtcacgggag gcgaggtcga ccccataacg gagcccggcc tcacgagcgc gttcctgcaa
     1681 ggcgccgacg gtgacccctg cccccacggt caccgatcga cgtgcgggat cgatcggccc
     1741 gacgcggtcg agtcttcgca aggagagcac gacctcccca cgatggggca ccgatccccc
     1801 gaccaatccg gtgttcccac cctgcggcac cacagacagc ccggttcgat gacagaggcg
     1861 gaccacctcc tcgacctcgg tgacggatcc gggccggacc accgccgcgg tccgtcccgt
     1921 ccaccggccg gtccaatcgc gcacgtagcc cgccaccatg tcgggctcgg tcaggacgtg
     1981 agtgtggcca acgatgcccc ggagcgcggc gaggacggct gcctcgtctc tcagcgacat
     2041 ctggcgactc tatgcatcgc cacctccaac ccctcgccgc cggcccgaca agggagcggt
     2101 cgcattaggt tggaggcctc agcgaggagc gcatgaccga atttcactat gaaccgttgt
     2161 cgtacctcga tgcgtccttc ctggccctcg agaccaagac cagccatatg cacgtcgccg
     2221 gcgtcgcgct cctcgacgcc gccccgctga aaggcgagga cggtggcatc gacatcgaac
     2281 gcatccgagc ccacgtcgag tcgaaactgc gctacatccc gcgttaccgg caacggctcc
     2341 aatgggtgcc gttcgaccgg cgaccggtgt gggtcgacga cgagcatttc gccatcgact
     2401 atcacctgcg tcatagctcg ctcccccgcc ccggcaccga ggcccagctc aagcagcttg
     2461 ccggccggat cgtgtccgcc cctctcgaca ggagcaagcc gctctgggag ctgtgggtcg
     2521 tcgagggact cgacgacgac cggttcgccc tcatcgccaa gatccaccac tgcatgatcg
     2581 acggcatctc gggagtcgat ctgaccacga tccttctcaa cgtcgtcccc agcgccgagg
     2641 tcgaggagcc cgaagtgtgg gtgccgcgtc ccg
//
LOCUS       DASTXO010000510         3069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_952432, whole genome shotgun sequence.
ACCESSION   DASTXO010000510 DASTXO010000000
VERSION     DASTXO010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952432"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1218)
                     /locus_tag="VFU96_10825"
     CDS             complement(<1..1218)
                     /locus_tag="VFU96_10825"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEU5113802.1"
                     /translation="MANTANMTIEKGVAIVGGGPAGLYLSILLRATNPELEVTVHERN
                     TPQDAYGFGVVFSDETLDNFENADPVSHRALVDEFRHWGRIRARHPEGVEIVSGGHGF
                     AAISRRTLLETLTGRALELGVRLRFSSEVTSPSDLGVAGVLVGADGANSTVRRELETD
                     LGFSIEHARNKYVWFGTPKVFDEFNFIFEDTPSGMVWAHIYPYGAEGSTFIVEMRPET
                     WSALGFEKTQDEVFPPGASDDYALQRCEEIFASHLDGLGLLGNNSRWLQFPTIGCERW
                     HHGNVVLMGDAVHTAHFSVGSGTKLAMEDAIALAGQLNADQPLEEAFARYEEERRPEV
                     ESLQRAAKASRDWFEGADRYRNMEPEQFVFSMLTRSQRITYDNLRVRDPGYMSGIDVW
                     YASSEHSCPGPVGP"
     gene            complement(1232..1999)
                     /locus_tag="VFU96_10830"
     CDS             complement(1232..1999)
                     /locus_tag="VFU96_10830"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5113803.1"
                     /translation="MPERSFGRTVRYRRTRIGLSQAQLGELVGRSASSIRSWERDVST
                     PTDPSVLNALSAILDIDGRALFDKAGVTTPAEEAHPTVEESLASLAPLPLEEPIEETI
                     DPDPLIASTAGVAAVEARLDADSELEAEVQEHLPSEPEPNDELAEEPEPAIDTFYLPP
                     EPEPQPEPVGAVQMSSSSEPAYVSPPEPYLITAPTPPVVEPSYMEDDSQRQMYRVRNL
                     ATVVLVVALAIILLWSLGNALDAVGTWWEEFTNTLRL"
     gene            2096..2191
                     /locus_tag="VFU96_10835"
     tRNA            2096..2191
                     /locus_tag="VFU96_10835"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2131..2133,aa:Sec,seq:tca)
     gene            2263..2673
                     /locus_tag="VFU96_10840"
     CDS             2263..2673
                     /locus_tag="VFU96_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113804.1"
                     /translation="MDIAIIVLAAVGVVFAGVALNRAKPNVRAERVPGHPDTFELWNE
                     GPGGALIVHADVLSPSFPRGASIDDAQSAIPGLWVDERILRDHGPWAKGASLDPLRRF
                     EIRLPAATSLRIVYRADGALGTVATATMTVEGGH"
     gene            2765..>3069
                     /locus_tag="VFU96_10845"
     CDS             2765..>3069
                     /locus_tag="VFU96_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013732126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-opening dioxygenase LigA"
                     /protein_id="HEU5113805.1"
                     /translation="MRRTASIASIVLGVLMIAGGIATWFVVSDTLADQQITTSDDACL
                     PGRAVTGPFTAYCQAKVIEQHALEATGGLYYAELDREDPLREVAMNAAFLQSSLFTSV
                     "
BASE COUNT          530 a         1012 c          959 g          568 t
ORIGIN      
        1 cggcccgacc ggtccagggc aggaatgttc ggagcttgcg taccagacgt cgattccaga
       61 catgtagccg gggtcgcgaa cccgcaggtt gtcgtatgtg atccgttggc tccgggtcag
      121 catgctgaac acgaactgct ccggctccat gttccggtaa cgatccgctc cctcgaacca
      181 atcacggctc gccttggccg cccgctgcag actctccact tcgggacgac gctcctcttc
      241 atagcgcgca aacgcctctt ccaagggttg gtcggcgttc agctgcccgg ccagggcgat
      301 cgcgtcctcc atggcgagct tggtgcccga gcccaccgag aaatgggcgg tgtggaccgc
      361 gtctcccatc agcaccacat ttccgtgatg ccaacgctcg cagccgatcg tggggaactg
      421 gagccagcgt gagttgttgc cgagcagacc gaggccgtcg aggtgggagg cgaagatctc
      481 ctcacaccgc tggagggcgt agtcgtcgct cgccccaggc gggaagacct cgtcctgtgt
      541 cttctcgaag ccaagggccg accaggtctc cggtcgcatc tccacgatga acgtgctgcc
      601 ctccgcaccg tatgggtaga tgtgagccca gaccatcccc gagggggtgt cctcgaagat
      661 gaagttgaac tcgtcgaaca ccttgggggt gccgaaccag acgtacttgt tccgagcgtg
      721 ctcgatcgag aagcccagat ctgtctccag ctcccggcga accgtgctgt tggcaccgtc
      781 tgcgccgaca agcacgccgg ccacccccag gtcggatgga ctcgtcacct cgctgctgaa
      841 gcgcaggcgc acaccgagct cgagagcacg ccccgtgagt gtctcgagca acgtgcgacg
      901 gctgatggcg gcaaagccgt ggccgcccga cacgatctcg accccttccg gatggcgtgc
      961 ccttatccgc ccccagtgcc ggaactcgtc gaccaacgcc cgatgcgaga ccgggtcggc
     1021 gttctcgaag ttgtccagcg tctcatcact gaacaccacc ccgaagccat aggcatcctg
     1081 aggcgtgttt cgctcatgaa ctgtgacctc gagctcggga tttgtcgccc ggagcaggat
     1141 cgacagatag agaccggccg gtcccccacc gacgatggcg acgccctttt caatggtcat
     1201 gttggctgtg ttggccaacg ctagagggct gttagagacg gagcgtgttg gtgaactcct
     1261 cccaccacgt gcctactgcg tcgagagcat tgcccaatga ccaaaggagg atgatggcga
     1321 gggcaaccac caggaccacg gtggccaggt tccggacccg gtacatctgg cgctggctgt
     1381 cgtcctccat gtacgagggc tcgacgaccg gtggagtggg ggccgtgatc aagtacggct
     1441 caggcgggct gacgtatgcg ggctcgctcg agctgctcat ctgcactgct cccaccggct
     1501 cgggttgagg ctcaggttcc ggcgggaggt aaaacgtatc gatcgccggc tctggttcct
     1561 cggcgagctc gtcgttgggc tccggctcgg aggggaggtg ctcctgcacc tcggcctcga
     1621 gctcggaatc ggcgtcgaga cgagcttcga ctgcggcgac ccccgccgtc gatgcgatga
     1681 gcgggtcggg gtcgatggtc tcctcgatgg gctcttcgag gggaagcggg gcaaggcttg
     1741 ccaacgattc ctcaacggtg ggatgcgcct cctcggcggg cgtcgtgaca ccggccttgt
     1801 cgaagagggc gcgcccatcg atgtcgagaa tggccgagag agcattgagc accgaggggt
     1861 cggttggtgt ggacacatcg cgttcccagc tcctgatcga gctggccgag cggccgacaa
     1921 gctccccaag ctgagcctga ctgaggccga tcctggtgcg gcgatagcgc acggttcttc
     1981 caaatgagcg ctcgggcatc aggccgaata gtaccggtgt gtgaaatttg ctccacgtgg
     2041 attcgaccgc tcgaagagca cccagaccgc agccctatgc tccttcgacc accgtggagg
     2101 cgtccggacg cctggtgggt cccccggtct tcaaaaccgg tgaggggtct gatcggcccc
     2161 tggcgggttc gattcccgtc cgcctccgcc atcatctctc ctaccgaatc gggtcgatag
     2221 aaccattgaa gcgaacccga cccgtcatag catttctcag aaatggacat cgcgataatc
     2281 gtccttgccg cggtcggtgt cgtgttcgct ggtgtcgccc tcaatcgggc caagccgaac
     2341 gtgcgcgccg agcgcgtccc cggtcatccc gacactttcg agctctggaa cgaaggtccg
     2401 ggcggagctc tcatcgtcca tgccgacgtg ctgtccccga gctttccccg cggagcctcg
     2461 atcgacgatg cacaatcggc catacctggt ctctgggtcg acgagcggat cctcagggac
     2521 catggcccat gggcaaaagg ggcctccttg gaccctctcc ggcgcttcga gataaggctc
     2581 ccggcggcca ccagcctgcg tatcgtctac cgggctgacg gcgcgctcgg cacggtggcc
     2641 acggcgacca tgacggtcga aggtggtcac tgagccgatt cccggagaca agtgactttc
     2701 ggccctcccc gcctctgtcg acacctgtag gtttgacccc gcccccgacg aaaggtgtcc
     2761 agccgtgaga agaaccgcct ccatcgcatc catcgtcctc ggtgtcttga tgatcgccgg
     2821 cggcattgcc acctggttcg tcgtgagcga cacactcgcc gaccagcaga tcaccacctc
     2881 tgacgacgcc tgcctgccgg gccgcgccgt gaccggccca ttcactgcct attgccaagc
     2941 gaaggtcatc gagcaacacg cgctcgaggc caccggaggc ctctattacg ccgagttgga
     3001 ccgtgaggac ccgctccggg aggtggctat gaacgctgcg ttcctccagt cctcgctctt
     3061 cacctcggt
//
LOCUS       DASTXO010000511         2455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1504825, whole genome shotgun sequence.
ACCESSION   DASTXO010000511 DASTXO010000000
VERSION     DASTXO010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504825"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..236
                     /locus_tag="VFU96_10850"
     tRNA            165..236
                     /locus_tag="VFU96_10850"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:197..199,aa:Thr,seq:cgt)
     gene            263..934
                     /locus_tag="VFU96_10855"
     CDS             263..934
                     /locus_tag="VFU96_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113806.1"
                     /translation="MPDSDPILDKMPHSARRWLSANFELISVIVMSVTAIATAWSGFQ
                     SSQWSAVQATSYGDAANDQTESVRVSNEARQATSIDLAVFIAWLEATTDGDEETAEFI
                     YEGMPERLQIATDAWLVEDPFDNPDAPTSPFVMEAYVVPAAQIADDLALSAEEHTLTA
                     QEATERANDYVLTTVLFATVLFFASISSKLRGVANRWAILSIAMIGLLAGGMILLAFP
                     RAPTA"
     gene            971..1549
                     /locus_tag="VFU96_10860"
     CDS             971..1549
                     /locus_tag="VFU96_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020557296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5113807.1"
                     /translation="MADQANFERDAMQYARQLYSAAMRMTRNPADAEDLVQETFLKAY
                     RAYHTFEEGTNLKAWLYRILTNTYINKYRKDSRRPSEVDLGSVEDLYLYRNIGSEESA
                     EAARTTEDRVLDGLVESDIKKAVEDLPENFRLPVLLADLEGFSYKEISDILDIPIGTV
                     MSRLHRGRKAMQKSLWEYAVNRGLVPEGAERP"
     gene            1546..1788
                     /gene="rsrA"
                     /locus_tag="VFU96_10865"
     CDS             1546..1788
                     /gene="rsrA"
                     /locus_tag="VFU96_10865"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HEU5113808.1"
                     /translation="MSGHECDDALTHLYQYLDREIEATTSEVIRAHLEDCSGCLRSFD
                     FEARLQVVVRERLAEEVPTEFLERLRDAIAREAASS"
     gene            1812..2129
                     /locus_tag="VFU96_10870"
     CDS             1812..2129
                     /locus_tag="VFU96_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113809.1"
                     /translation="MKRLSRIATLVVLVAVHASIFVVPALATEGTQEQTSTTVAEEPA
                     PASGVEPAVPMTTPQPTESLPDWTYRYMIPTGIVLAVLVIVMTTIQYFTRVVRKRYRI
                     VKE"
     gene            2126..>2455
                     /locus_tag="VFU96_10875"
     CDS             2126..>2455
                     /locus_tag="VFU96_10875"
                     /inference="COORDINATES: protein motif:HMM:NF021608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEU5113810.1"
                     /translation="MTSSRSGSGRLAGRIAGTYPLAETYHRSALEADMTRLTGQVSDL
                     VPAATGLDLPGDPTTIVIGREEWIERNVASFSHLMKPLQRQLTEKMEAAGPRGRSVAT
                     FTNRLVQA"
BASE COUNT          472 a          800 c          735 g          448 t
ORIGIN      
        1 caccgagcca gtccgggtca gtagaagact tcctgcctca gcaagggagg cgagaaccca
       61 tagcgcctcc ggcccacccc ccaagtcgga ggcgctctcg cgcaacactc ctttcatgat
      121 ggtctggctc agtgcccact ctccctttac cattgagtcc ccccgccgga gtagctcagt
      181 ggtagagcaa cgcactcgta atgcgtaggc caggggttcg actcccctct ccggctcgct
      241 gaccgctcgt tagattccgc tggtgccgga ctccgacccg atcctcgaca agatgccgca
      301 ctcggcccgt cgctggttgt cggccaactt cgagctgatc tcggtgatcg tgatgtcggt
      361 gacggcgatc gccaccgctt ggtcgggttt tcagagctcg caatggtcgg cggtccaggc
      421 gacgagctac ggcgatgcag ccaatgacca gaccgaatcg gtacgcgtct ctaacgaggc
      481 ccgacaggcc acctcgatcg atctggccgt tttcatcgcc tggctcgagg caactaccga
      541 cggtgacgag gaaacggccg aattcatcta cgaagggatg ccagaacgtc tgcagatcgc
      601 gaccgacgcg tggttggtcg aagacccgtt cgacaatccg gacgccccga cgtctccctt
      661 tgtcatggag gcgtacgtgg tccctgcggc gcagatcgcc gacgaccttg ctctgtcggc
      721 cgaggagcac accttgactg ctcaggaggc cacagagcgc gccaacgact atgtgctgac
      781 gactgttctc ttcgccacag tcctcttctt cgcttcgatc agctccaagc tccgcggagt
      841 agccaacagg tgggcgatcc tgtcgatagc aatgatcggt ctgctggccg gagggatgat
      901 cctgctggca tttcccaggg cgcctaccgc ttagggcttt cgacccgcac cctgtaccgt
      961 cgctcctcaa atggcggacc aggccaactt cgaacgcgat gccatgcagt acgcgcgcca
     1021 gttgtacagc gccgccatgc gcatgactcg caacccggca gatgccgagg acctggtcca
     1081 ggagacattt ctcaaggcct accgcgccta tcacacgttc gaagagggga ccaacctcaa
     1141 ggcctggctc taccgaattc tcaccaacac ctacatcaac aagtatcgca aggactcgcg
     1201 gcggccctcc gaggtcgacc tgggctcggt cgaggatctc tatctctacc ggaacatcgg
     1261 ctcagaggag tcggcggagg cggcgcgcac caccgaggac agggtgctgg acgggctcgt
     1321 ggagtcggat atcaagaagg cggtcgagga cctgcccgag aatttccggc tccccgtatt
     1381 gctggccgac ctcgagggtt tctcatacaa ggagatctcc gacatccttg acatcccgat
     1441 cggtaccgtc atgtcgcggc tccaccgggg aagaaaggcg atgcagaaat cgttgtggga
     1501 atacgcagtc aacagaggcc tggtgcctga aggcgcggag cgaccatgag cggccatgag
     1561 tgcgatgacg cactgaccca tctctatcaa tatctcgaca gggagatcga agccaccaca
     1621 tccgaggtga tccgcgccca tctcgaagac tgttcgggat gcctgcgcag cttcgatttc
     1681 gaagctcgac ttcaggtcgt ggtgagagag cgccttgccg aggaggtccc aacggagttc
     1741 ctggagcggt tgcgcgacgc gatcgcccgc gaggcggcat cgtcctagtt gagcactatt
     1801 ctgcgcgtca gatgaagcga ttgagccgca tcgcgacact cgtcgtcctc gtcgccgtcc
     1861 acgcatccat attcgtcgtg ccggccctgg cgaccgaggg gacgcaggag cagacgtcga
     1921 ccacggtggc cgaggagcct gccccggctt cgggcgtcga gccggccgtg ccgatgacca
     1981 ctccgcagcc gaccgagtcg cttcccgact ggacctaccg atacatgatc ccgacgggga
     2041 tcgtccttgc cgtcttggtc atcgtcatga ccacgatcca gtacttcacc agggtcgttc
     2101 gcaagcggta ccgaatcgtc aaagagtgac ttccagccgg tccggatcgg gccgtctcgc
     2161 cggaaggatc gcagggacct atcccctcgc tgagacctac caccgatctg cgctcgaggc
     2221 cgacatgaca cgcctcaccg gccaggtctc agatctcgtg ccggcggcca ccgggctcga
     2281 ccttcctggt gatccgacca ccatcgtgat cgggcgggag gagtggatcg agcgaaacgt
     2341 ggccagtttc agccatctga tgaagccgct gcagcgccag ctcaccgaga agatggaggc
     2401 tgcgggccct cggggacgca gtgtggccac attcaccaat cggctggtcc aggcc
//
LOCUS       DASTXO010000512         3399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_878997, whole genome shotgun sequence.
ACCESSION   DASTXO010000512 DASTXO010000000
VERSION     DASTXO010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878997"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1382)
                     /locus_tag="VFU96_10880"
     CDS             complement(<1..1382)
                     /locus_tag="VFU96_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017579734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HEU5113811.1"
                     /translation="MPSSSTTESSQATFEDVVSAFVAHHPDGDVDLLRRAYEVAAEAH
                     ADQVRKTGDPYITHPLAVAHMLAGYGLDEPTLAAALLHDTVEDTNLTLNDLSMQFGDE
                     IGRLIDGVTKLDRVRYSNREQAQAATIRKMVVAMAQDVRVLIIKLFDRLHNLRTVHAL
                     REEKQRRVAQETLDVYAPLAHRLGVQEIKHEFEDRCFAILYPGPKAEIQAKLAERAPE
                     RQAFIEKMVDEIAGILADSGIEAEVTGRPKHQYSIYRKMREQNRPFEDIHDLIGIRII
                     TETLRDCYAALGLIHSHWVPVTGRFKDYIAMPKINLYQSLHTTVIGPDGKPLEVQIRT
                     AEMHQRAEAGIAAHWRYKEGEAGPLPRMEFLSWGDEEDPEEFLANLKLDLYQDEVFVL
                     TPGGDVKPLPAGSTAIDFAYSVHTEVGHRCVGARINGRLVPLSTRLQSGDIIEVLTSK
                     SVEAGPSRDWL"
     gene            complement(1360..1878)
                     /locus_tag="VFU96_10885"
     CDS             complement(1360..1878)
                     /locus_tag="VFU96_10885"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017625766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEU5113812.1"
                     /translation="MENLTALIRDIPDFPEPGIVFKDITPVLADPAAFATLVEALAEP
                     FRDAGVTKVAGIEARGFTLATPVAGLLEAGFIPVRKPGKLPYETVREDYSLEYGTDSL
                     EIHTDAAQPGEKVLLVDDVIATGGTAAAAIRLLRKIGADVVGFAVFIELSFLDGAQML
                     DGVPLHALVKYD"
     gene            complement(1899..2213)
                     /gene="yajC"
                     /locus_tag="VFU96_10890"
     CDS             complement(1899..2213)
                     /gene="yajC"
                     /locus_tag="VFU96_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU5113813.1"
                     /translation="MTHLVFAQETAASPLPSLLFLGLMVAVFWLFIIRPQRQRAKAQQ
                     ELSKSISPGEEVRTIGGIHGIVVSVDEESVVLRVEEGKIRVSRKAVGSRVGPDKPDAE
                     TP"
     gene            complement(2270..3379)
                     /gene="tgt"
                     /locus_tag="VFU96_10895"
     CDS             complement(2270..3379)
                     /gene="tgt"
                     /locus_tag="VFU96_10895"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU5113814.1"
                     /translation="MDAVTWTSQARDGAARAGWLDTPHGRIPTPAFMPVGTRAAVKAV
                     DSHDLAGVGAEIVLANTYHLMLRPGSSRIAVLGGLHRFMAWDGPILTDSGGFQIFSLE
                     PIIEERGARFRSVYDGSQVELTPEDAVRIQEELAPDIAMALDVCVGLPAPTELVEAEL
                     ERTLRWADRCLRAHTRPDQALFGIVQGGVDPDLRAKSAAQTAALGFPGFGIGGLSVGE
                     GRTERHLALEASFAELPDGKPRYVMGLGDTDGLLEAIGRGADMFDCVLPTRLARHGKV
                     LTRTGDFSLRNAVSETDDCPIDEECACHTCRTHSRAYLRHLVRVKELSAHRLLTIHNL
                     RYTLDLVAGARVAIESGTFSDFVAEVAQTRANGSP"
BASE COUNT          585 a         1076 c         1123 g          615 t
ORIGIN      
        1 agccagtcac gactgggccc ggcctccacc gacttcgagg tcagcacttc gattatgtcc
       61 cccgactgga ggcgggtcga gagtgggacg agccggccat tgatcctggc accgacgcac
      121 cgatggccga cttcagtgtg caccgaatag gcgaagtcga tggcggtgga cccggcaggt
      181 agtggcttca cgtcgccgcc cggggtgagg acgaacacct cgtcctggta caggtcgagc
      241 ttgaggttgg ccaggaactc ttcgggatct tcctcgtccc cccaggagag gaattccatc
      301 cggggcaatg gcccggcctc gccctccttg tatcgccaat gggcggcgat gcctgcttcc
      361 gccctctggt gcatctcggc agtgcggatc tggacttcga gcggcttgcc atcaggacca
      421 atgaccgtgg tgtggagcga ctggtagagg ttgatcttgg gcatcgcgat gtaatccttg
      481 aagcgcccgg tcaccgggac ccagtgactg tggatcagcc cgagcgcggc ataacaatcc
      541 cgcagcgtct cggtgatgat ccggatgccg atcaggtcgt ggatgtcctc gaacggccgg
      601 ttctgctccc tcatcttgcg ataaatcgag tactggtgct tcgggcggcc ggtcacttcg
      661 gcctcgatgc cggaatcggc caggatgccg gcgatctcgt cgaccatctt ctcgatgaat
      721 gcctgacgct cgggggctcg ctcggccagc ttggcctgga tctcggcctt gggccccgga
      781 tagaggatgg cgaagcagcg gtcctcgaac tcatgcttga tctcctggac tccgagcctg
      841 tgagccagcg gtgcgtagac atcgagggtc tcctgtgcca cccgtcgctg cttctcctcg
      901 cgcaacgcat ggacagtcct caggttgtgg agacggtcga acagcttgat gatcaggaca
      961 cggacgtcct gggccatggc aacgaccatc ttgcggatgg ttgccgcctg ggcctgctcc
     1021 cgattcgagt aacggactcg atcgagcttg gtcacaccgt cgatgagccg tccgatctcg
     1081 tcgccgaact gcatagagag atcgttgagc gtgagattgg tgtcttcgac ggtgtcatgg
     1141 agcagggcag cggccagcgt cggttcatcg agcccgtagc cggccaacat gtgggccacc
     1201 gccagcggat gcgtgatata ggggtcgcca gtcttccgca cctgatcggc atgcgcctcg
     1261 gctgctacct cataagccct tcggagcagg tcgacgtccc catcggggtg atgggcgacg
     1321 aaggcactga cgacatcctc gaatgtcgcc tggctcgact cagtcgtact tgacgagggc
     1381 atgcaggggt acaccgtcca gcatctgggc gccgtcgagg aacgacagct cgatgaaaac
     1441 cgcgaaccca accacgtcgg cgccgatctt cctcaacaac cggatcgcgg cggctgctgt
     1501 tccaccggtc gcgatcacat cgtcgacgag gaggaccttc tccccgggct gggcggcgtc
     1561 ggtgtggatc tccagtgagt cggtgccgta ttcgagcgag tagtcctcgc ggaccgtctc
     1621 gtaaggcagc tttcccggct ttcgaaccgg tatgaagccc gcctcgagga gaccggcaac
     1681 gggagtggcg agggtgaagc cccgcgcctc gattcccgcg accttggtca ctccggcgtc
     1741 gcgaaacggc tccgccaggg cctcgaccag cgtggcaaag gcggccgggt cggcgaggac
     1801 cggcgtgatg tccttgaaga cgatcccggg ctcgggaaag tcggggatgt cgcgaatcag
     1861 cgcggtgagg ttttccaccc cggaagtcta cggtctgatc agggagtttc ggcgtctggc
     1921 ttgtccggcc ccacccggga cccgacggct ttccgcgaga cgcggatctt tccctcctcc
     1981 acccggagaa cgacgctctc ctcgtcgacc gacaccacga tgccgtggat cccgccgatg
     2041 gtccgaacct cttcgccggg gctgatggac ttcgacaact cctgttgggc tttggcgcgt
     2101 tgacgctgag gccggatgat gaacaaccag aagacggcaa ccatcaaccc gaggaagagc
     2161 agcgagggca gcgggctcgc cgcggtctcc tgagcaaaga cgagatgggt cactggatcc
     2221 tccagatcgc gagagacgga cccgccggag gttaggcaat gccaggggtc tagggcgaac
     2281 cgttcgcgcg ggtctgcgct acctctgcga cgaagtcgga gaacgtcccc gattcgatgg
     2341 ccacccgtgc ccccgcgacc agatcgagcg tgtagcggag gttgtggatg gtgagcaacc
     2401 gatgagccga cagctccttc acacggacca ggtgacgcag gtaggcccgg ctatgggttc
     2461 ggcaggtatg acaagcgcat tcttcgtcga tagggcaatc gtcggtctcg gatacggcgt
     2521 tgcgaaggct gaaatcgcca gtgcgtgtca ggacttttcc atgtctggcg agcctggtcg
     2581 ggagaacgca gtcgaacata tccgctcccc ttccgatcgc ctcgagcagc ccgtcggtgt
     2641 ctcccagccc catcacgtat cgaggcttgc cgtccgggag ctcggcgaaa cttgcctcga
     2701 gggccagatg ccgctctgtg cgaccttccc cgacggacag ccccccaatt ccgaagccgg
     2761 ggaacccgag ggcggcggtc tgggctgcgc tcttcgccct gagatccggg tcgaccccgc
     2821 cctggacgat cccgaacaga gcctggtccg gtctggtgtg cgcccggagg cagcgatcgg
     2881 cccagcgcag ggtccgctcc agctccgctt cgaccagctc ggtgggtgcg gggaggccga
     2941 cacacacatc gagggccatg gcgatgtcgg gggccagctc ctcctggatc ctgaccgcgt
     3001 cctcgggcgt gagctcgacc tgtgagccgt cgtagacgga ccggaatcgg gcgccacgct
     3061 cctcgatgat cggttcgagg gagaagatct gaaacccgcc ggaatcggtc aggatgggcc
     3121 cgtcccatgc catgaaccga tggagcccgc cgaggacggc gatccgggag gaaccgggcc
     3181 ggagcatcag gtggtaggtg ttggccaaca cgatctcggc tccgaccccg gcgaggtcgt
     3241 ggctgtccac ggccttgacc gctgccctgg tcccaaccgg catgaaggcc ggagtgggga
     3301 tcctgccgtg aggggtgtcc agccatccgg cgcgggctgc cccatcccta gcctgcgagg
     3361 tccaggtcac ggcgtccacg atctgattat cgctcggcg
//
LOCUS       DASTXO010000513         3594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1247262, whole genome shotgun sequence.
ACCESSION   DASTXO010000513 DASTXO010000000
VERSION     DASTXO010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247262"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VFU96_10900"
     CDS             <1..635
                     /locus_tag="VFU96_10900"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HEU5113815.1"
                     /translation="DDIAAANGIVNGTVYTGTRLFLDGPAFVGEGVGGSASYVVQSGD
                     RLGDIAAKHGTNISALADLNGLSDINLIRAGQELAVPGGSNWVCPVDGGRYFNDWGFP
                     RGGSRFHEGNDIFAERGTPVRAPVSGTVELIEGTVGGLQFNLYGSDGIKYLGSHLDAS
                     GETGDVGAGDVIGYVGTSGNAQGTNPHLHFGMYRDGAAINPHPSLVSNGC"
     gene            complement(653..2311)
                     /locus_tag="VFU96_10905"
     CDS             complement(653..2311)
                     /locus_tag="VFU96_10905"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEU5113816.1"
                     /translation="MPETTPFEICDRLTEAYADLSPIAATEAGVPGRDHLWDDFSPDG
                     EVAKEELRAKAVAELATHFDHPDAVQRTAARVASGYLASERRLYDSGHWKRNINHIHG
                     PFQTARDTFDIVPKEGAEAWENVTIRLSTWGDMLDGYRSSLQMGLDEGEIVAHRQVLS
                     VIDQVRSVAGPASRFSSLPGLAAADGGDEEGVAGAVSRARSASAGFADWLETVYLPRA
                     VPSDAAGLDRYAEESERFLGMRLDVDDTYDWGWSEVHRLLGEMRETAAEIDAGLNIDE
                     VIDLLDNDPGRSAPDHEAFADFVREIQAHAVDRLDGTAFDVPPPMCDVTVNLAPPGGA
                     LGAWYVAPSEDLDRAGSIWYALGERTRIPYWQEVATAYHEGFPGHHLQVGIAVLQREK
                     LSRFHRLFVWYPGSGEGWALYAERLMEELGFFENPEYRLGLQASQLFRSARVVVDIGT
                     HLEKRIPDDAPMHAGEVWNYELAVDYMEKIGRQPRDLAESEVLRYLGWPGQAISYKVG
                     EREILEIRKEAMTEAGASFDGKDFHRRLLDAGAIRLDQLRDTMA"
     gene            2359..3405
                     /locus_tag="VFU96_10910"
     CDS             2359..3405
                     /locus_tag="VFU96_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEU5113817.1"
                     /translation="MEDIIEVRPDERFDVGRLTGWMAGYRALPDGSPTVRQFAGGKAN
                     LTYLLQFPGGEQLVLRRPPLGPVAESSHDMTREYTVLSRLWQAFDKAPRAVALCDDGS
                     VIGTPFFLMEPKPGVVIRDVVPDLFGGGRDEGVNRKLSSVVIDTLVELHAVDAAECGL
                     EGLGHPDGFLERQVEGWWKRWELAKDDDDPVADEVGDWLRSDIPSSGPPTLLHNDWRL
                     DNMAVSPADPGACVAVYDWDMATRGDPLADVGTLMASWYDDGEEKSTLAPMPTTSPGW
                     LDRRSAIERYGERSGRDLRHVDWYVVFGTWKLGVVLQQIYIRWLRGQTKDPRFEPLGG
                     GARALFRLAARRRA"
     gene            3470..>3594
                     /locus_tag="VFU96_10915"
     CDS             3470..>3594
                     /locus_tag="VFU96_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015747054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5113818.1"
                     /translation="MTIPKLAGADTDDPAQGLQAVVVLRRTTERLEAIHVAAARA"
BASE COUNT          632 a         1175 c         1158 g          629 t
ORIGIN      
        1 ccgacgacat cgccgctgcg aacggaatcg tgaacgggac ggtctacacc ggcacacgac
       61 tcttcctcga cggtccagcc tttgtcggcg agggagttgg aggctcggcc agctacgtgg
      121 tgcagtcagg tgaccgtctc ggcgacatcg ccgccaagca cggcaccaac atctcggccc
      181 tggccgacct gaacggtctg tccgacatca atttgatcag agccggtcag gaactcgccg
      241 tgccgggcgg gtcgaactgg gtttgccctg tcgacggggg acggtacttc aacgattggg
      301 gcttcccccg tggcggctct cgcttccacg agggcaacga catcttcgcc gagcggggta
      361 ccccggtgcg tgccccggtg tcgggcacag tcgagctcat cgaggggact gttggggggc
      421 tccaattcaa tctgtacgga agtgatggga tcaagtacct cggctcccac ctcgacgcgt
      481 cgggtgagac cggcgacgtc ggcgcaggtg acgtcattgg atatgtgggc acgtcgggca
      541 acgcccaagg gacgaatccc cacctccact tcgggatgta tcgggacgga gcagcgatca
      601 atcctcaccc gtcgctcgtg tccaacggct gctgagtctc gccccgccct ggtcaggcca
      661 tcgtgtcgcg cagctgatcg agcctgatcg ctccggcgtc gagcaggcgt cggtggaaat
      721 ccttcccgtc gaaagaggca ccggcctccg tcatcgcctc cttccggatc tcgagaatct
      781 cccgctcccc caccttgtag gagatggcct ggccgggcca ccccagatag cgaagtactt
      841 cggattcggc gagatctcga ggctgtcgac cgatcttctc catgtaatcc accgcgagct
      901 cgtagttcca cacctctccg gcgtgcatag gggcgtcatc cgggatccgc ttctccagat
      961 gggtgccgat gtcgacgacc accctcgccg acctgaacag ctgacttgcc tggaggccta
     1021 gccggtattc cgggttctcg aagaaaccga gctcctccat gagccgctcg gcgtacaacg
     1081 cccatccctc tcccgagccc ggataccaga cgaacaagcg gtggaatcgg gacagcttct
     1141 ctcgctgcag cactgcgata ccgacctgga gatgatgccc gggaaaaccc tcgtgatagg
     1201 cggtggcgac ctcttgccag tatgggatcc gggtcctctc gccgagggcg taccagatgg
     1261 atccggcccg atcgagatcc tcagacgggg cgacatacca ggctccgaga gcgccaccgg
     1321 ggggagcgag attcaccgtc acatcgcaca taggcggcgg cacgtcgaag gccgtcccgt
     1381 ccagccggtc cacggcgtgc gcctggatct cccggacgaa gtcggcaaaa gcttcgtgat
     1441 cgggggccga gcgaccggga tcgttgtcca gcaggtcgat gacctcatcg atgttcagac
     1501 cggcgtcgat ctccgcagcc gtttcccgca tctcaccgag gaggcgatgc acctcggacc
     1561 atccccagtc gtatgtgtcg tcgacgtcga gacgcattcc gagaaaccgc tcggactcct
     1621 ccgcgtagcg gtccagtccg gctgcgtcgg atggcacggc tcgcggcagg tagacggtct
     1681 cgagccagtc tgcgaagccg gccgaggccg atcgggcccg cgaaacggct cccgccaccc
     1741 cctcctcgtc gcccccatcg gcagccgcga gccctggaag cgagctgaag cgcgaggccg
     1801 ggccggcaac cgagcgcacc tgatcgatga cgctgagcac ctggcgatgg gccacgatct
     1861 ccccttcatc cagccccatt tgcaggctcg accgataccc atcgagcatg tcgccccagg
     1921 tcgagagacg aatcgtcacg ttctcccacg cctcagcccc ttccttcggc acgatgtcga
     1981 aggtgtcacg tgcggtctgg aacgggccgt ggatgtggtt gatgttccgc ttccaatgcc
     2041 cgctgtcata gagacggcgc tccgatgcca gatatccgct cgccacccgg gcagcggttc
     2101 tctgcaccgc gtccgggtga tcgaaatgcg tggctagctc ggccacggcc ttcgcccgca
     2161 actcctcttt cgccacctcg ccatcggggg agaagtcgtc ccagagatga tcacggccag
     2221 gaacgccggc ctcggtggcg gcgattggcg aaagatcggc gtaagcctcg gtgagacggt
     2281 cgcagatctc gaacggtgtg gtctctggca tgctcaggat gaggcgtgag ggtagacccc
     2341 ccggataccc ttcgatgagt ggaagatatc atcgaggtcc gacctgacga gcgattcgat
     2401 gtcggtcgcc tcaccggctg gatggcgggc taccgggcgc ttcccgatgg gtctccgacg
     2461 gtccggcagt tcgccggtgg caaggccaac ctcacctacc tccttcagtt tccaggcggt
     2521 gagcagctgg tgctgaggag accgcccctc ggccccgttg ccgagagctc tcacgacatg
     2581 acccgcgagt acaccgtcct ctcccgactc tggcaggcct tcgacaaggc gccgcgggcc
     2641 gtggcgctgt gtgacgacgg cagcgtcatc ggcaccccgt ttttcctgat ggagcccaag
     2701 ccaggtgtgg tcatccggga tgtcgtcccc gacctcttcg gcgggggcag agacgaaggc
     2761 gtgaacagga agctgtcatc ggtcgtgatc gacaccctgg tcgagcttca cgcggtcgac
     2821 gcggccgaat gcgggctgga gggcctcggg cacccggacg gcttcctcga acggcaggtg
     2881 gagggatggt ggaaacggtg ggagctggca aaggatgacg atgacccggt ggccgacgaa
     2941 gtcggcgact ggcttcgatc ggatatcccg tcgtcgggcc cgcccaccct ccttcacaac
     3001 gattggcgac tggacaacat ggctgtatca ccggccgatc cgggtgcctg tgtggcggtg
     3061 tacgactggg acatggccac gagaggagac cctctcgccg atgtggggac gttgatggcc
     3121 tcctggtacg acgacggaga ggaaaagtcg accctggccc cgatgcccac tacctcaccg
     3181 ggttggctcg acaggagatc tgctatcgag cgttacgggg aacgctccgg aagggacctg
     3241 cgccacgtcg actggtacgt cgtcttcggg acgtggaagc taggggtcgt cctccaacag
     3301 atctacatcc gatggctgcg ggggcagacg aaagacccac gcttcgagcc actgggaggc
     3361 ggggcccggg ctctgtttcg gctggccgcc cgcaggagag cctgagcagc tactgctatg
     3421 ggtcgtcaac cgaggttgac atgttctgct gtcaacctaa gatgacagcg tgacgatccc
     3481 caagttggcc ggagccgaca ccgacgatcc cgcccagggc cttcaggcgg tggtcgtgct
     3541 gcgccgcacg acggagaggt tggaggcgat ccatgtcgca gctgcccgcg ccca
//
LOCUS       DASTXO010000514         3309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1025752, whole genome shotgun sequence.
ACCESSION   DASTXO010000514 DASTXO010000000
VERSION     DASTXO010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025752"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="VFU96_10920"
     CDS             complement(<1..876)
                     /locus_tag="VFU96_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5113819.1"
                     /translation="MTTLAGTGKLIRLVLRRERLRLSIWVVVLAAVPIITANAFIDLY
                     PTQASRQALAATVISSPALTSMLGPLYDSSIGGLVAWRAGVLGAVFVALMAVLTVIRH
                     TREEEETGRRELLGATVVGRHAQLAAAVTVTILAGILVAALLTAGLSGLGLPMAGSLA
                     FGLGFAGVAATFAGVGAVAAQLTEAASSARGIAVSVVGLMYLLRVAGDAGGDATEWMS
                     WLSPIGWFSRLHPYAEEQWWVLMLWVGWATILIAAAVAIAARRDVAAGAFPSRPGPAQ
                     ASPRLSSPLGLAWRLH"
     gene            complement(873..1769)
                     /locus_tag="VFU96_10925"
     CDS             complement(873..1769)
                     /locus_tag="VFU96_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014178267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5113820.1"
                     /translation="MSTAISTTGLVKNFGHVRALDGLDMEVATGEVHGFLGPNGSGKT
                     TTIRVLLGLIRQDAGTAILLGGDPWSQATELHRRLAYVPGDVTLWPNLTGGEVIDLLG
                     RLRGGLDQPKKEKLLERFDLDPTKKGRTYSKGNRQKVALVAALSSDVELLILDEPTAG
                     LDPLMEEQFRKCIEEDRASGRTVLLASHILAEVEALCDRVSIVRKGKTVESGTLQELR
                     HLTRTTISAELSADPSPIASLPGVHDLRLHDHKVDFEVDSDRIDEVVRTLAPLGVRSL
                     VSHPPTLEELFLRHYEDEEVQA"
     gene            1820..2479
                     /locus_tag="VFU96_10930"
     CDS             1820..2479
                     /locus_tag="VFU96_10930"
                     /inference="COORDINATES: protein motif:HMM:NF036526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU5113821.1"
                     /translation="MRSRPNEDLTTRARIRDAALARFPADGFGGTTIRAVAADAGVSP
                     GLVVHHFGSKEGLREACDHHVVARFREIKLAAMEQDNVFDPAFAALTFVEAQPLLRYF
                     GWALSRNHPAAGDLFDEMVREGVEITRVAVGKGMVRDSPDLERRTAVQMALNLGMLVM
                     HAHLERNLGIDLLSADGIAELSPTLFEIFGGMFTPEVRDQIGEAYGAEARRLVAASIE
                     S"
     gene            2656..2931
                     /locus_tag="VFU96_10935"
     CDS             2656..2931
                     /locus_tag="VFU96_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113822.1"
                     /translation="MAVETSGHSRGNPRLYELIENAPKTGKSPTIWAWPEIVDYLKVA
                     GQPVQGPWPPHQQPRPDPSAESLPVAVPDPESSPRQPGPSPESGPQA"
     gene            2953..>3309
                     /locus_tag="VFU96_10940"
     CDS             2953..>3309
                     /locus_tag="VFU96_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEU5113823.1"
                     /translation="MYPSRFRYEAPRSVEEAMSLLSDGGGEAKVLAGGQSLVPMIKLR
                     FASPEMLVDINEIPGLDYHRVDPDGSIHVGALCRHADLERSSLLTSRQPTMASAAPLV
                     ADPIVRNRGTLVGSLCH"
BASE COUNT          589 a         1097 c         1095 g          528 t
ORIGIN      
        1 gtggagccgc caggcgaggc ccagcgggct gctgaggcgc ggagacgcct gggcaggccc
       61 cgggcgtgat gggaaggccc ccgccgccac gtcgcgtcgt gccgcgatcg ccacggcggc
      121 ggcgatcagg atggtcgccc atcccaccca cagcatgagg acccaccact gctcctctgc
      181 gtaggggtgg agccggctga accaaccgat gggcgagagc caggacatcc actcggtggc
      241 atcaccgccg gcgtcccccg ccacccggag caggtacatc agccccacga ccgacacggc
      301 gatgccgcgc gccgaggagg cagcctcggt gagctgagcg gcgaccgcgc caacgccggc
      361 gaacgttgcg gcgacaccgg caaacccgag cccgaatgcc aatgagccgg ccatggggag
      421 gccgagaccg gacaaaccag cagtcaacaa cgcggccacg aggatcccgg ccaggatggt
      481 cactgtgacc gcggccgcca gctgggcatg acgccccacc acagtggcgc cgagaagctc
      541 ccgcctcccg gtctcctcct cctcacgggt gtgcctgatc acggtgagaa ccgccatcaa
      601 ggcgacgaat accgcgccca agacccctgc ccgccatgcc accaggccac cgatgctcga
      661 gtcgtacagg gggcccagca tcgaggtgag ggccgggctc gagatgaccg tcgccgccag
      721 cgcctgacgc gaggcttgag tcggatagag atcgatgaat gcgttcgcgg tgatgatcgg
      781 gacggcagcc aacacgacga cccagatgct gagtcgaagc cgctcccggc gcaggaccag
      841 acggatcagc ttccccgtcc cggccagggt ggtcacgcct gcacctcttc gtcctcatag
      901 tgccgaagga acagctcctc gagcgtgggc gggtggctga ccaggctgcg cacgccgagc
      961 ggagccaggg tccgaaccac ttcgtcgatc cggtcggagt cgacctcgaa gtccaccttg
     1021 tggtcgtgga gacgcagatc gtgaacgcca ggcagcgagg caatcgggga agggtcggcg
     1081 ctcaactcgg cgctgatcgt ggtccgggtc agatggcgca gctcctggag cgtgccgctt
     1141 tcgacggtct tgcccttgcg aacgatgctg acccggtcgc agagcgcttc gacctccgcc
     1201 aggatgtggc tggccaggag gaccgtccgc ccggaggccc ggtcttcctc gatgcatttg
     1261 cggaattgtt cctccatgag cggatccaga ccggcggtcg gttcgtcgag tatcagcagc
     1321 tccacgtcag aggagagggc ggctaccagc gccaccttct gccggttgcc cttcgaatag
     1381 gtgcgtccct tcttggtggg gtcgaggtcg aacctctcga ggagcttctc tttcttcggc
     1441 tggtccagac caccacggag ccgaccgagg aggtcgatca cctcaccacc ggtcaggttg
     1501 ggccagagcg tcacgtcgcc ggggacatac gccagccggc gatggagctc ggtggcctgt
     1561 gaccaggggt ctccacccag aaggatggcg gtacctgcat cctggcggat gagcccgagc
     1621 aacaccctga tggtggtggt cttgcccgat ccgttgggcc cgagaaagcc gtggacttcg
     1681 cctgtggcca cctccatgtc gagcccgtcg agcgcccgta catgcccgaa gttcttcacc
     1741 aggccggtgg ttgagatcgc agtgctcatg atcaagaatc gtagcaccct tgcttgaaca
     1801 cacgtttagt atgttgacca tgcgttcaag gccgaacgaa gacctgacca cacgagcccg
     1861 gatccgggac gccgccttgg cccgcttccc cgccgacggc ttcggcggga cgacgatccg
     1921 agccgtcgca gccgatgccg gggtatcccc aggcctggtc gttcaccatt tcgggtccaa
     1981 ggagggactg cgggaggctt gcgaccatca cgtcgtggcc cgtttccgcg agatcaagct
     2041 ggcggcgatg gaacaggaca acgtcttcga tcccgccttc gccgccctga cattcgtcga
     2101 ggcacaaccc ctgcttcggt actttggctg ggccttgtcc cgcaatcacc cggccgccgg
     2161 tgatctgttc gacgagatgg tccgggaagg ggtcgagatc acccgggtgg cagtggggaa
     2221 agggatggtc cgtgactcgc cggacctgga gcgacggacc gcggtccaga tggcgctcaa
     2281 cctcggcatg ctcgtcatgc acgcccacct ggaacggaac ctgggcatcg acttgctgag
     2341 tgcggacggg atcgccgagt tgtccccgac ccttttcgag atcttcggcg ggatgttcac
     2401 ccctgaggtg cgggaccaga tcggggaggc ctacggagcc gaggcacgga gattggtcgc
     2461 ggcaagcatc gaatcctgaa tggcgcggaa ccggaacgtg ggccgcgacg taatgggcga
     2521 gaaggccgac gcggaagtcg ccgattgcgt aaggttcgga tcgggcagga ttccgggaag
     2581 accgggatcc tttgtgcgat gggaggcagg ccgtggttcg cgtcgcggtc ggcctatgag
     2641 actgagaggt gacccttggc agtcgagaca tccggtcata gccgcggcaa cccgcggctg
     2701 tacgagctga tcgagaacgc ccccaagacg gggaagagcc ccacgatctg ggcgtggccg
     2761 gagatcgtcg attacctcaa agtcgccggg cagccagtcc agggcccctg gcctccacat
     2821 cagcagccga ggcctgaccc gtcggccgag tctcttcccg tagcggtgcc cgatcccgaa
     2881 tcttcccctc gtcagcccgg cccctccccc gaatccgggc cacaggcctg aagcacaccc
     2941 aaggaggcag atatctaccc ctctcgattc cgctacgagg caccgaggtc ggtcgaggag
     3001 gccatgtcac tcctgtccga cggtggcggg gaggccaagg tcctcgccgg tggccagagc
     3061 ctcgtcccga tgatcaaact gcgcttcgca tcgccggaga tgcttgtcga catcaacgag
     3121 atccccggtc tcgactatca ccgggtcgat cctgatgggt cgatacacgt cggggccttg
     3181 tgccgtcacg ccgacctcga gcgctcgtcg ctcctgacaa gccggcaacc gaccatggct
     3241 tcggcggcgc ctctggtggc cgacccgatc gtcaggaata gagggacgct cgtcggatcg
     3301 ctctgtcat
//
LOCUS       DASTXO010000515         3378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_473437, whole genome shotgun sequence.
ACCESSION   DASTXO010000515 DASTXO010000000
VERSION     DASTXO010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473437"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1635
                     /locus_tag="VFU96_10945"
     CDS             <1..1635
                     /locus_tag="VFU96_10945"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU5113824.1"
                     /translation="LSPLGEDLIEAVEAEWIPVRPSTDTAVMLGLAHSLLVSGTHDED
                     FLQLYCVGWDRLRAYLLGEPDGVPKTADWAGGISGVDPDRLRALAMDMAARRTLITVS
                     WSVQRNDHGEQPYWMAAALAAMLGQIGLPGGGVGYGYGAVGLNGHPVGYHTLPRLSQG
                     KNPVVDFIPVARIADLLLHGGEDFDYNGKRHTYPDIHLVYWAGGNPFHHHQDLNRLAQ
                     AWQIPDTMICHEPFWNSLARRCDIVLPATTPLERTDIGGSGNDDFIFWMEQVIEPVGE
                     ARNDYEIFSDLARRLGVDVRFTEGRTAEEWLEHIYTRFRAHHPEYPALGELREAGHFQ
                     IPEDWIPPLVSQIVAFRRDPRGSPLKTPSGRIELFSQTIEEFGYDDCPPHPSWLEPAE
                     WLGNAGRYPLHMISNQPKTRLHSQWDHGETSLNGKVDGREQIGISPSDAAARGLQTGD
                     LVRVFNQRGACLAAASIRADLMPGVVQLATGAWWDPTEPDGMCRAGNPNVLTRDAGTS
                     RLAQGPTAQTCLVEVEAYGGSPPAVLAHQPPRFASE"
     gene            complement(1636..2301)
                     /locus_tag="VFU96_10950"
     CDS             complement(1636..2301)
                     /locus_tag="VFU96_10950"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEU5113825.1"
                     /translation="MKIVDLTVPIGRDTYSPPSVNQPITIEEYHKEPGFWVVSEITMM
                     LHAGSHVDFTKHYDADGETAETILLDRTCGEAVLIDLTPIEPDHDITPEDLAQAAPEI
                     RPGDVVLVRTGWSDLAWGDFPRYYVGSPACSPEAAQWLVGTGAKAIGFDCFPERAAKK
                     QSYLPSEFVIHEIIGDSGAVLMQTLTNLGALPTEGRFLFYAAFLKVSGGEGAPTRFFA
                     VLD"
     gene            complement(2309..2929)
                     /locus_tag="VFU96_10955"
     CDS             complement(2309..2929)
                     /locus_tag="VFU96_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113826.1"
                     /translation="MSDTADRVNAASSTEKAHIDELLYLARHLDKVYQRVEEMEFGVD
                     LRTDEHPLLVLQGSGVVELRAGGRAARIDSQVIDYRAPDRSAYPDRTHHDVFVTEPGA
                     QKVIESDGATIITTQRVLYNSPTWNRSWDYAKAAEIFHTDYVSQGWGASFIGVSNRAR
                     TSGFMYRSGFACSVRDRLVLALAVADGTVEDMVLALKAERSELDRS"
     gene            complement(3019..>3378)
                     /locus_tag="VFU96_10960"
     CDS             complement(3019..>3378)
                     /locus_tag="VFU96_10960"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5113827.1"
                     /translation="GQTIYGASKAAVKLMTEGLYAELLDTNVGVSVIFPGAVATEITS
                     NSGVEIPGNIPNEEEMRARTTSPEDAARIILDGIEENDLQIFVGRDSRMMNLFNRVSP
                     RRSTHLIYKQMKDLLSS"
BASE COUNT          602 a         1069 c         1084 g          623 t
ORIGIN      
        1 ctctcgccgc tgggcgaaga tctgatcgaa gccgtggagg ccgagtggat ccccgtccgg
       61 cccagcaccg acacagcagt gatgctcggg ctcgcccaca gcctcctcgt ctctgggacg
      121 cacgatgagg atttcctgca gctctactgc gtcggctggg accggctgcg cgcctatctg
      181 ctcggggagc ccgatggcgt gccgaagacg gcggactggg cgggcggcat cagcggggtc
      241 gacccggatc gccttcgagc gctcgccatg gacatggctg cgaggcgcac actgatcacg
      301 gtcagctggt cggtgcagcg aaacgaccac ggggagcagc catattggat ggccgccgcg
      361 ctggccgcga tgctgggcca gatcggcctc ccgggtggtg gagtcggcta cggctacggg
      421 gcagtgggcc tgaacgggca tccggtgggt tatcacaccc ttccccgtct ctctcaaggc
      481 aagaacccgg tggtggattt catcccggtg gcccggatcg cggacctcct gctccacggt
      541 ggcgaggact tcgattacaa cggcaagagg cacacctacc cggacatcca cctcgtctac
      601 tgggcgggtg gcaacccctt ccaccatcat caggacctga accgcctggc ccaggcctgg
      661 cagatcccgg acacgatgat ctgtcacgag ccgttctgga attcccttgc ccgacgttgc
      721 gacatcgtcc tgcccgccac cactccactg gaacggaccg atattggcgg aagcgggaat
      781 gacgacttca tcttctggat ggaacaggtc atcgagccgg tgggtgaagc gagaaacgac
      841 tacgaaatct tctccgatct cgccaggcgc ctcggggtcg atgttcgatt caccgagggc
      901 aggacggctg aggagtggct ggagcacatc tacacacggt tccgggccca tcatcccgag
      961 tacccggcac tcggtgagtt gcgggaagcg gggcattttc agatcccgga ggattggatc
     1021 ccgcccctcg tctcccagat cgtcgccttc cgccgtgacc cgcgtggctc gccgctgaag
     1081 acgccttcgg gccggatcga gttgttctcg cagaccatcg aggagttcgg ctacgacgac
     1141 tgcccgccac atccttcctg gctggagccg gccgaatggc ttggaaacgc aggtcgctat
     1201 cccctccaca tgatctcgaa tcagccgaag acacggcttc actcccagtg ggaccacggg
     1261 gagaccagtc tcaacggcaa ggtcgacggc cgggagcaga tcgggatcag cccgtcggat
     1321 gccgcggcgc gcggtctcca aaccggtgac ctggtacgtg tcttcaacca gcggggcgca
     1381 tgtctggctg cggcgtcgat cagagcagac ttgatgcccg gcgtcgtcca gctggccacc
     1441 ggagcctggt gggaccccac cgagcccgac ggaatgtgtc gggccggcaa tcccaatgtt
     1501 ctgacgcgtg acgccgggac ctcgaggctg gcccagggac cgacggcaca gacctgtctg
     1561 gtcgaggtgg aggcatacgg cggatcgccg ccggcagtgc tggctcatca gccgccccga
     1621 ttcgccagcg aataatcagt cgaggacggc gaagaaacgg gtcggggcgc cttctccacc
     1681 gctgaccttc aggaacgccg cgtagaacag gaacctgccc tcggtcggca atgcgcccag
     1741 attggtcagc gtctgcatga gcaccgcacc cgagtcgccg atgatctcgt ggatcacgaa
     1801 ctcgctcggc aggtagctct gcttcttcgc cgcccgctcc gggaagcagt cgaacccaat
     1861 ggctttggcc ccggtgccca ccagccactg ggccgcctcc gggctgcatg ccggtgagcc
     1921 aacgtagtag cggggaaaat ccccccaggc gaggtcggac catccggtcc ggaccagcac
     1981 gacgtcgccg ggtcggatct cgggagcagc ttgggccagg tcctcgggcg tgatgtcgtg
     2041 gtcaggctcg atcggcgtga gatcgatgag cactgcctcg ccacacgtgc ggtcgaggag
     2101 gattgtctcg gccgtttccc catcggcgtc gtagtgcttg gtgaagtcga catgtgagcc
     2161 ggcgtggagc atcatcgtga tctcggacac cacccagaat ccgggttcct tgtggtactc
     2221 ctcgatcgtg atcggctgat tcaccgacgg cgggctgtag gtgtccctcc cgatggggac
     2281 ggtcaaatcg acgatcttca tcgtgacgtt atgaccgatc cagctcggat ctctccgctt
     2341 tgagcgcgag gaccatgtcc tcgacggtgc cgtcggcaac agccagggct agaaccagac
     2401 gatcccgcac cgaacaagca aagccgcttc gatacatgaa ccccgaggtt ctcgcccggt
     2461 tggacacgcc gatgaaggag gctccccagc cctggctcac atagtcggtg tggaagatct
     2521 cagctgcctt ggcgtaatcc caggaacggt tccaggttgg ggagttgtag aggactcgtt
     2581 gcgtggtgat gatcgtcgcg ccgtctgact cgatgacttt ctgcgccccg ggttccgtga
     2641 cgaagacgtc atggtgggtg cgatcggggt aggccgaacg gtctggagcg cgatagtcga
     2701 tcacctgaga gtcaattctc gccgcccttc cgccggctct gagctcgaca acgccggagc
     2761 cctggaggac caggagcggg tgctcatcag tcctgaggtc gacgccgaac tccatttcct
     2821 cgacccgttg gtagaccttg tcgagatgcc gggccaggta gagaagctca tcgatgtgag
     2881 ccttctccgt gctcgaggcc gcattgacgc ggtcggccgt gtcggacact tggcgtcccc
     2941 taaaaagatc gcgaacctcc caggtccccg ctcgatccgg agcctaatca tggtggtggc
     3001 gcgagcgcgc gccggacctc aggaagagag caggtctttc atctgcttgt agatcagatg
     3061 ggtcgaccgc cgcggtgaga cgcgattgaa caggttcatc atccgtgagt cgcggccgac
     3121 gaagatctgg agatcgtttt cctcgatgcc atcgaggatg atcctggccg cgtcctcggg
     3181 cgacgtagtg cgcgctcgca tctcctcctc attggggatg ttccctggga tctctacccc
     3241 cgagttagaa gtgatctcgg tggccacggc gccagggaag atcaccgaga caccgacgtt
     3301 ggtgtcgaga agctcggcgt agagcccttc agtcatcagc ttgactgctg ccttggatgc
     3361 tccgtagatc gtctgacc
//
LOCUS       DASTXO010000516         2800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_695217, whole genome shotgun sequence.
ACCESSION   DASTXO010000516 DASTXO010000000
VERSION     DASTXO010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695217"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..474
                     /locus_tag="VFU96_10965"
     CDS             79..474
                     /locus_tag="VFU96_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEU5113828.1"
                     /translation="MPLPRGLARFNRVVTNRLSRPLASWAPGFALIVHRGRRSGLEYR
                     TPVNAWVGDEDVIVALTYGRDTDWLKNLAAADGGLVTAGRETHQVGRPELIGPEGMSR
                     MPAPARPILRLIEVNEFALLPLIRPDRPT"
     gene            complement(452..1486)
                     /gene="tdh"
                     /locus_tag="VFU96_10970"
     CDS             complement(452..1486)
                     /gene="tdh"
                     /locus_tag="VFU96_10970"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEU5113829.1"
                     /translation="MRALVKHADEVGLRLDDVSIPEPGPDDILIRVLTTGICGTDLHI
                     FNWDDWARATIPAPMTVGHEFSGEIVALGPNVSGFEVGELVGAEGHVVCNRCRNCLAG
                     RRHLCHDARGIGVNLPGAFADYVLIPKSNVWRHQEGLDPEIAAIFDPFGNAVHTALEF
                     EVLGEDVLITGAGPIGLMACAVVAHAGARNVVVTDVNPFRLDLAKRMGATRAVDIRSE
                     DLHDIQVEMGMSEGFDVALEMSGSPSAMEDILQNTIHGANVAILGIPTDPFPIDWSIV
                     IFNMLNLKGIYGREMFETWYKMNVLIQSGVDISPVITHRFEVEDFDAAFEATASHDSG
                     KVLIRWADPA"
     gene            1556..2083
                     /locus_tag="VFU96_10975"
     CDS             1556..2083
                     /locus_tag="VFU96_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008745982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEU5113830.1"
                     /translation="MEGTLGGQLLVATPLLVDPNFWRSVVLILQHDDDGSLGLVLNRP
                     TVERVETHIPVWGPVAAKPGTVHFGGPVEPRVAIGLATGRDGEPTGVPGLSIVDLEAE
                     PGERTSAVRIYSGYAGWGQGQLEMEIAEGSWYVVPASPDDPFDRPDGQWSRILRRQNG
                     HLALVSTFPDDVDLN"
     gene            2123..>2800
                     /locus_tag="VFU96_10980"
     CDS             2123..>2800
                     /locus_tag="VFU96_10980"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU5113831.1"
                     /translation="MTTTAPKLRPSLKRKAPGLIVERGLRDRGLEVVVGMDEVGRGSW
                     AGPLTVGAAIVPLDRRVYKIRDSKMLTEDEREALFDRIAGWCDAWAVGHATPEECDSL
                     GMSDAQRLAAQRALAGLGAVPDHVLLDGKWDFVGGATRIVRGDRTSLSIAAASILAKV
                     TRDRLMRGLADQYPGYNFEANKGYPCPVHKSALQAWGPSAIHRRSWVFMDYLMWNGLE
                     RYHRPEPQ"
BASE COUNT          504 a          896 c          909 g          491 t
ORIGIN      
        1 acgagatcct caacgagccg gagggaccgg agctggtcca ggagatcacc gcctggatcg
       61 actcccggat ctgagatcat gcccttgccc cgagggctcg cacggttcaa ccgcgtggtc
      121 accaatcgtc tcagccgccc cttggccagt tgggcccccg ggttcgcctt gatcgtccat
      181 cgcggccgga gatccggcct cgagtaccga accccggtca atgcctgggt cggtgatgaa
      241 gacgtcatcg tggccctgac ctatgggagg gacaccgatt ggctgaagaa tctggccgca
      301 gcagatggcg ggttggtgac cgccggtcgg gagacccacc aggtggggcg gccagagctg
      361 atcgggccgg aaggaatgag ccgcatgccg gcgccggcga ggccgatcct tcgcctgatc
      421 gaggtcaacg agtttgccct gttgcccctg attaggccgg atcggcccac ctgatcagca
      481 ccttgcccga gtcgtgagag gcggtcgcct cgaacgcggc atcgaaatcc tcgacctcga
      541 agcgatgtgt gatcaccggt gagatgtcga cgcccgattg gatgagaacg ttcatcttgt
      601 accaggtctc gaacatctca cgcccataga tccccttcag gttgagcatg ttgaagatga
      661 cgatgctcca atcgatgggg aaggggtcgg tcgggatgcc gagaatcgcc acattggctc
      721 cgtggatggt gttctggagg atgtcctcca ttgccgaagg gctaccggac atctcgagtg
      781 cgacatcgaa accctcggac atccccatct cgacctggat gtcatggagg tcctccgacc
      841 tgatgtcgac ggcgcgggtc gctcccatcc tcttggccag gtcgaggcga aacgggttga
      901 catcggtgac caccacattt cgggcaccag cgtgggccac caccgcacag gccatcagcc
      961 cgatcgggcc ggcccccgtg atcaggacat cttcgccgag cacctcgaac tcgagcgcgg
     1021 tgtggaccgc atttccgaag gggtcgaaga tggctgcgat ctccgggtcg agaccttcct
     1081 ggtgccgcca gacattcgac ttggggatca agacatagtc ggcgaacgca ccggggaggt
     1141 tgaccccgat cccgcgggcg tcgtggcaca ggtggcgtct cccggcaaga caattgcggc
     1201 agcgattgca gaccacatgg ccctctgcac ccaccagctc accgacttcg aaaccggaga
     1261 cgttggggcc cagcgccacg atctcacccg agaactcatg cccgactgtc atcggggcag
     1321 ggatcgtcgc ccgcgcccag tcgtcccaat tgaagatgtg cagatcggtg ccacagatac
     1381 cggtcgtgag gaccctgatc aggatgtcgt caggcccggg ctcgggaatc gagacgtcat
     1441 cgaggcgcag cccaacctca tctgcgtgtt tcaccagtgc cctcatgaac ccgaacgtta
     1501 aatgagggag cgccgctgtt cgcacgaatc ctcggggctg tggcacgatg tcgtcgtgga
     1561 aggaaccctc ggtggtcagc tcctggtggc gacaccgttg ctggtcgacc ccaatttctg
     1621 gagatcggtc gtgttgatcc tccagcacga cgacgacggc agtctcggtt tggtcctcaa
     1681 ccgaccgacc gtcgaacgtg tcgagaccca catcccggtg tggggccccg tcgccgccaa
     1741 gccgggcacc gtccatttcg gcggcccggt ggagccacgt gtggcgatcg ggctcgccac
     1801 cggtcgggat ggcgagccca ccggagtacc cggcttgagc atcgtcgacc tcgaggcgga
     1861 gccgggggag cggacttccg ccgttcgcat ctattcggga tacgcaggct ggggtcaagg
     1921 ccagttggag atggagatcg cggaaggctc gtggtacgtc gtcccggcct cgcccgacga
     1981 cccattcgac cggcccgacg ggcagtggtc tcgaattctc cgtcgccaga acggccacct
     2041 cgccctcgtc tccacatttc ccgacgatgt cgacctcaac tgaccaccgc gtctcggtcg
     2101 tcgggcccta ccctgaccgg acatgaccac gacggctccc aagctgcggc ccagtctcaa
     2161 gcgcaaggcg ccgggtctca tcgtcgagcg tgggctccgg gatcggggac tcgaggttgt
     2221 ggtaggcatg gatgaagtgg gaagagggtc atgggccggg ccactcacgg tcggcgcagc
     2281 catcgtcccc ctcgaccgga gggtgtacaa gataagggac tcgaagatgc tcaccgagga
     2341 cgagcgggag gccctgttcg accggatcgc agggtggtgc gatgcctggg cggtcggcca
     2401 cgccactccg gaggagtgcg actccctcgg aatgtcggac gcgcagagat tggctgcgca
     2461 gcgcgccctc gccgggttgg gtgcggtgcc cgaccacgtc ttgctcgacg ggaaatggga
     2521 tttcgtcggc ggagctacgc gcatcgtgag aggcgatagg acctcgctct cgatcgccgc
     2581 cgcctcgatc ctggccaagg tcacccgcga ccggctgatg agaggcctgg ccgatcagta
     2641 tcccggctac aacttcgagg cgaacaaggg ttacccgtgc ccggtgcaca aatcggctct
     2701 ccaagcctgg gggccctcgg ccatccatcg ccgatcctgg gttttcatgg actacctcat
     2761 gtggaatggg ttggagcgct accaccggcc cgagccacag
//
LOCUS       DASTXO010000517         3240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1726568, whole genome shotgun sequence.
ACCESSION   DASTXO010000517 DASTXO010000000
VERSION     DASTXO010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1726568"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="VFU96_10985"
     CDS             complement(<1..595)
                     /locus_tag="VFU96_10985"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEU5113832.1"
                     /translation="MASDRLKTRLGPIALRSPLIAASGTVGSVWEWAEVADVSPYGAA
                     VAKSVAPVEWPGRPAPRLAPAGPGMLNGIGIQNPGIEAWVGKMTPLIGGLEVPVWGSA
                     VGESPDEFALVAKGLASAGVQAVEVNLSCPNLEDGRMFSLDASRSAEVIATVTSTCEV
                     PVGAKLSPNADDIVGVASACLEAGATFLTLTNTILGFG"
     gene            complement(577..>3240)
                     /gene="carB"
                     /locus_tag="VFU96_10990"
     CDS             complement(577..>3240)
                     /gene="carB"
                     /locus_tag="VFU96_10990"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014909243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU5113833.1"
                     /translation="GLATSPVSEILVEESVEGWKEYELEVMRDHKDNAVIVCSIENLD
                     PMGVHTGDSITVAPIQTLSDREYQEMRDEGIAVLRAIGVETGGSNVQFAVDPATGRRL
                     VIEMNPRVSRSSALASKATGFPIAKIAALLAVGYTLDEISNDITGATPASFEPALDYV
                     VVKVPRFDFSKFPSAPTRLGTSMRSVGEAMAIGRTFPEALQKALRSMENGRHGLNADP
                     GEQTLLDMGDEELGQAIAIPTPNRIFAIAEGLRRGWGVTEVSRLSSVDPWFVDQMAEI
                     VEVRSDVEAAGPGSREILEEAKRYGFSDRQIAHLWGVGEVQVREARARLGVNRTYKTV
                     DTCAAEFEANTPYMYGTFEEESEVPPADRPRVVVLGSGPNRIGQGIEFDYCCVHAAFA
                     LRDAGYETVMVNCNPETVSTDYDTSNRLYFEPLTIEDVLSVIEAERPEAVIVQLGGQT
                     PLNLARALQDAGVPIAGTSPASIELAEDREQFAELCKSLGLRQPPAGSASSAREAEDV
                     VDQVGLPVMVRPSFVLGGRKMRVVYSVEELADYVREIYGEADPELTGAPILIDRFLEA
                     AVEIDVDAVYDGSELFLGGVMEHVEEAGVHSGDSDCVTPPPTISDEAHKEILRATSEL
                     AAALEVRGLINLQFAVKGDDVYVLEANPRGSRTVPFISKATAVPLARIAARVMMGSSL
                     EDLRREGVVPVSAHRPDFVAVKNAVLPWNRFPEEDSVLGPEMRATGEVMGIGPDVGVA
                     FAKALLAAGHILPTRGRVFLSFADRDKPMGLAVAQAFTMLGFDLVATHGTARYLKHHA
                     IEAEPVHKVGEGPRDIGQRLEDGEVQLVINTPRGGKARSDGRTIRIAARTHSIPCVTT
                     IQGGLAVARSLRAGPGAIQRPRSLQEWHQTV"
BASE COUNT          542 a         1113 c         1043 g          542 t
ORIGIN      
        1 tgccgaatcc caggatggtg ttggtcaggg tgagaaaggt ggcgcctgct tccagacacg
       61 cggaggccac acccacgatg tcgtccgcgt tgggcgagag cttggcccca accggcacct
      121 cgcaggtcga ggtcacggtg gcgatgactt cggccgaccg cgatgcatcg agtgagaaca
      181 tgcgcccgtc ttcgaggttg gggcaggaga gattgacttc gacggcctgc acgcccgccg
      241 aagcgagccc tttggccacc aaggcgaatt catccgggct ctccccgacc gcggagcccc
      301 ataccggcac ctcgagacca ccgatcagtg gcgtcatctt ccccacccac gcctcgatgc
      361 ccgggttctg gatcccgatc ccgttgagca tcccgggacc ggcaggggcg aggcgcggcg
      421 cgggccgccc cggccattcc acgggcgcga ccgacttggc gacggcggca ccgtacggag
      481 acacgtcagc cacctctgcc cactcccaga ccgacccgac tgtgcccgag gcggcgatga
      541 gcgggctacg caacgcgatc gggcccaggc gcgtcttcag acggtctgat gccattcctg
      601 gaggctcctc ggtcgctgta tggcgccggg cccggcgcgg aggcttcgcg ccactgccag
      661 cccgccctgg atggtggtca cacacggaat ggagtgggtg cgagccgcga tccgtatcgt
      721 cctgccatcc gagcgcgcct tcccgccacg cggggtgttg atgacgagtt gcacctctcc
      781 gtcctcgagc cgttgcccga tgtccctggg cccctcaccg accttgtgga ccggctcggc
      841 ttcgatggcg tgatgcttca ggtagcgggc ggtgccatga gtcgcaacca ggtcgaaccc
      901 cagcatggtg aaggcctggg ccacggccag gcccatcggc ttgtcccggt cggcgaagga
      961 gagaaacaca cgtccacgcg tcgggaggat gtggccggcc gcgagcaagg ccttggcgaa
     1021 ggccactccg acgtcgggcc cgatccccat aacctcaccg gttgcccgca tctccggccc
     1081 cagcaccgag tcctcttcgg ggaatcggtt ccaggggagg accgcgttct tcacggcgac
     1141 gaagtcgggg cggtgcgccg agaccggaac cacgccctcg cgacgtagat cttccagcga
     1201 gctgcccatc atgaccctgg ccgcgatgcg ggccaggggc acggcggtgg ccttgctgat
     1261 gaacgggacc gtcctgctcc cccgcgggtt ggcctccagg acgtagacgt catctccctt
     1321 caccgcgaat tggagattga tcaggccgcg cacctcgagc gcggcggcca actccgacgt
     1381 tgcccggagg atctccttgt gggcctcgtc cgagatggtc ggcggtggcg tgacgcagtc
     1441 ggaatccccc gaatgcacac ccgcctcctc gacatgctcc atcacaccac cgaggaacag
     1501 ctcagacccg tcgtacacgg catcgacgtc gatctcgacc gccgcctcga ggaaacgatc
     1561 gatgagaatc ggcgcaccgg tcaactccgg gtcggcctcg ccatagatct cccgcacgta
     1621 atcggcgagc tcttcgacgc tgtacaccac ccgcatcttg cggccgccga gcacgaacga
     1681 aggccggacc atgaccggca gaccgacctg gtccacaacg tcctctgcct cccgcgccga
     1741 tgaagccgag cctgccggcg gctgacgcaa gcccaggctc ttgcagagct cggcgaactg
     1801 ctcacgatcc tcggccagct caatcgacgc cggggaggtg ccggcgatgg ggacaccggc
     1861 atcctgcagc gcccgggcca ggttgagcgg ggtttgccct ccgagctgca cgatgacggc
     1921 ttcgggacgc tcggcctcga tgacggacaa cacgtcctcg atggtcaggg gctcgaaata
     1981 gagccggttc gaggtgtcat agtcggtcga aaccgtctct ggattgcagt tcaccatcac
     2041 ggtctcgtac cccgcgtcgc gcaaggcgaa tgcggcgtgg acacagcaat agtcgaactc
     2101 gatgccctgg ccgatccggt tcggccccga tccgaggacc accacgcgtg ggcggtctgc
     2161 gggggggacc tccgattcct cctcgaacgt cccgtacatg tatggggtgt tggcctcgaa
     2221 ctcggcggcg catgtatcca cggtcttgta ggtccggttc actccgaggc gggcccgagc
     2281 ctcccgcact tggacttcgc caacgcccca gagatgggca atctgacgat cggagaagcc
     2341 atatcgcttc gcctcctcga ggatctcacg gctccctggg cccgcggctt cgacatccga
     2401 ccgaacctct acgatctcgg ccatctgatc gacgaaccat gggtctaccg agctgagccg
     2461 cgagacctcg gtcacacccc atcccctgcg caggccctcg gcgatggcaa agatgcgatt
     2521 cggggttggg atggcgatgg cctggcccaa ctcctcgtcg cccatgtcga gcagggtctg
     2581 ctcacccgga tcggcgttga ggccgtgacg cccgttctcc atgctgcgaa gggccttctg
     2641 gagcgcctca gggaaggtcc tgccgatcgc catcgcctcg ccgacgctcc gcatcgacgt
     2701 tccgagccgg gtgggtgccg acgggaactt ggagaagtcg aagcgaggca ccttgacgac
     2761 cacgtagtcc agcgctggct cgaaggaagc gggtgttgca ccggtgatgt cgttggagat
     2821 ctcgtccagg gtgtatccga cggcgagcaa ggcggcgatc ttggcgatgg gaaaccccgt
     2881 cgccttgctg gccagagccg acgagcggct cactctggga ttcatctcga tcacgaggcg
     2941 gcgcccggtg gctggatcca ccgcgaactg gacgttggag ccgccggtct cgactccgat
     3001 agcccggagc accgcgatcc cttcgtcgcg catctcctgg tactcgcggt cggacagcgt
     3061 ctggatcggc gccacggtga tcgagtctcc ggtgtggaca cccatcggat cgaggttctc
     3121 gatcgagcaa acgatcaccg cgttgtcctt gtggtcgcgc attacctcga gctcgtactc
     3181 cttccagccc tcgacggact cctcgacgag tatctccgag accggggagg tggcgaggcc
//
LOCUS       DASTXO010000518         2545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1247431, whole genome shotgun sequence.
ACCESSION   DASTXO010000518 DASTXO010000000
VERSION     DASTXO010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247431"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VFU96_10995"
     CDS             complement(<1..378)
                     /locus_tag="VFU96_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113834.1"
                     /translation="MTIHRPINRRMAIAEMGKAGLAIVVFGTAACSDGGSDATTTAPG
                     TIPTTTTGPSTSPTAPGSTTTQGSTTTSGAASTTGAAATSYQRVNLQFVSAYILYRGG
                     EAALVDTGVGGSTAAIQASLGEAG"
     gene            442..1800
                     /locus_tag="VFU96_11000"
     CDS             442..1800
                     /locus_tag="VFU96_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEU5113835.1"
                     /translation="MLTKITLPETELPTHWYNIVPDLPVPPPPVLHPGTHEPVGPDDL
                     APLFPMDLILQEVSSERFIEIPEEIREVYRMWRPTPLYRAHQLEKLIGGPARIYYKYE
                     GVSPAGSHKPNTAVAQAYYNAKAGVKKLSTETGAGQWGSALSFACALFGLECEVFMVR
                     SSFDQKPYRKAMIETWGATVHSSPSELTQAGRSILEADPDTPGSLGIAISEAVEVAAT
                     DDDTKYSLGSVLNHVLMHQTVIGEEVLKQLALVEETPDVVIGCAGGGSNFAGFSFPFV
                     REKLAGNMDPVVRAVEPAAAPSLTRGSYQFDFGDTVGMTPLVKMHTLGHDFVPDPIHA
                     GGLRYHGMAPLVSLLKEEGIIEAESLHQTETFGRAVQFARAEGIIPGPEPTHAVASAM
                     MEAERAKEAGEEKVIVFNLSGHGHFDMAAYQEYLGGTLIDYEYPEERIAAAMERVPVV
                     SG"
     gene            1829..>2545
                     /locus_tag="VFU96_11005"
     CDS             1829..>2545
                     /locus_tag="VFU96_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase"
                     /protein_id="HEU5113836.1"
                     /translation="MTRLTDLSGADLQQMLDVLSGADSVELKLTLLETKRAGVAQALG
                     IDPLDAQLRQVFFLDTKDLTLNQAGVVVRARRRQGEKGDTVVKIRPVDPAELPDELRA
                     DPAFGVEVDAMPGGYVCSASYKGSASNEEIKQVASGERPARKLFSKSQRAFFAEHAAA
                     DLGLDDLEVLGPIPTLRVKFQSEGFKRPMVGELWLYPDGSQVVELSTKTPPAEAFQTA
                     AEWRAFLDRHGVELTGEQQTK"
BASE COUNT          454 a          803 c          849 g          439 t
ORIGIN      
        1 accggcctcc ccgagggagg cctggatcgc ggctgtgctt cctccgaccc cggtgtcgac
       61 cagggccgct tctccacccc gatagaggat gtaggccgag acgaattgga ggttcacgcg
      121 ctggtatgag gtcgcggcgg ccccagttgt ggaggcggcg ccactggttg tggtcgagcc
      181 ttgtgtggtc gtcgatccgg gggccgtagg gctcgtactc ggcccggtgg ttgtagtcgg
      241 gattgtcccc ggcgcggtgg tggtggcgtc tgagcctcca tccgagcagg ctgcggtgcc
      301 gaagacgacg atggcgagac cggctttccc catctcggcg atcgccatgc gacgattgat
      361 cgggcggtgg atggtcatag gcgtccggat cggggacaga gccaaaggta cccgggatct
      421 gtggttgtgt agcttcccgt catgcttacc aagatcaccc tgccggagac cgagctcccc
      481 acacattggt acaacatcgt tcccgacctg ccggtgccgc cgcctcccgt gctgcacccg
      541 ggaacccacg aaccggtcgg cccggacgat ctcgccccgt tgttccccat ggacctgatc
      601 ctgcaggaag tgagcagcga gcgattcatc gagatccctg aggagatacg cgaggtctac
      661 cggatgtggc gccccacgcc tctgtatcgc gcccaccaac tcgagaagct gatcggcggc
      721 ccggctcgca tctactacaa gtacgaggga gtcagcccgg ccggctcgca caagccgaac
      781 acggccgtgg cccaggccta ctacaacgcc aaggccgggg tgaagaaact ctccaccgag
      841 accggggccg ggcagtgggg ctcggcgctc tccttcgcct gcgccctttt tggcctcgaa
      901 tgcgaggtgt tcatggttcg gtcctcgttc gaccagaagc catatcgcaa ggcgatgatc
      961 gagacgtggg gtgccacggt tcattcctcg ccatcggagt tgacacaggc cgggcgctcg
     1021 atcctggagg cggacccgga cacacccgga agcctcggca tcgccatctc cgaggctgtg
     1081 gaggtggcag ccaccgacga cgataccaaa tactccctgg gctcagtcct caaccatgtc
     1141 ctcatgcacc agacagtcat cggcgaggag gtgctcaagc agctcgccct ggtggaggag
     1201 acccccgatg tcgtcatcgg ctgtgccggg ggcgggtcga attttgccgg gttcagcttt
     1261 ccgttcgtca gagagaagct ggccgggaac atggatccgg tggtgcgtgc cgtggagccg
     1321 gccgcggcgc catcactgac ccgggggagc taccagttcg atttcggcga cactgtcggg
     1381 atgaccccgt tggtgaagat gcacaccctc ggccacgact tcgtgcccga cccgatccat
     1441 gccggaggcc ttcgctatca cgggatggcg cctctcgtgt ccctgctcaa ggaggagggg
     1501 atcatcgagg ccgaatcgct gcaccagacg gagacgtttg gccgagcggt ccagtttgcc
     1561 cgggcggagg ggatcatccc cggaccagag cccacccatg cggtggcctc ggcgatgatg
     1621 gaagcggagc gggccaagga ggcaggtgag gagaaggtga tcgtcttcaa cctgtcgggg
     1681 cacggtcact tcgacatggc cgcatatcag gaatacctgg gcggcaccct catcgactat
     1741 gaataccccg aggagcgcat cgccgcggcc atggagcggg tgccggttgt gagtggctga
     1801 atccgcgttt gcgagacaag ggagagagat gacccggttg accgacctgt ccggagccga
     1861 cttgcagcag atgctcgatg tcctttccgg ggccgatagc gtcgaactga aactcacact
     1921 gctggagaca aagagagctg gggtggctca ggcgttgggt atcgatcccc tcgatgctca
     1981 gctccgacag gtgttcttcc tggacaccaa agacctcact ctcaaccagg cgggagtggt
     2041 ggttcgggcc cgccggcgcc agggcgagaa gggcgacacc gtggtgaaaa tccgcccggt
     2101 cgaccccgca gagctgcctg acgagctccg ggccgatccc gcgttcggcg tcgaggtcga
     2161 cgccatgccc ggcggctatg tctgctcggc gtcctacaag gggtcggcca gcaacgagga
     2221 gatcaagcag gtcgcctcgg gtgaacgccc ggcgcgcaag ctcttctcca agagccaacg
     2281 cgccttcttc gcggagcacg ccgccgccga cctcggtctg gacgatctcg aggtcctggg
     2341 gccgatccca accctccggg tcaaattcca atcggaaggg ttcaagcgcc cgatggtcgg
     2401 cgagctttgg ctctatcccg acgggtcgca ggtcgtcgag ctatcgacca agacaccacc
     2461 ggccgaggcg tttcagactg cagcggagtg gcgggcgttc ctcgacaggc atggcgtcga
     2521 gctcacggga gaacagcaga ccaag
//
LOCUS       DASTXO010000519         2336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1284152, whole genome shotgun sequence.
ACCESSION   DASTXO010000519 DASTXO010000000
VERSION     DASTXO010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284152"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VFU96_11010"
     CDS             complement(<1..194)
                     /locus_tag="VFU96_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011603586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU5113837.1"
                     /translation="METWDAITARRATREYDDRPLTDEDLERVLEAGRRAPSSRNNQR
                     WDFVLIRDREQLEQISKVWQG"
     gene            complement(235..>2336)
                     /locus_tag="VFU96_11015"
     CDS             complement(235..>2336)
                     /locus_tag="VFU96_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014689092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU5113838.1"
                     /translation="SHGFICPKGSTLGKIDEDPDRVRVPLIKWDGRHEPASWEEAFTA
                     IESRLLPIIADHGNSSVGVYLGNPNVHSMSGTLYPRVFLKMLGTKSIFSAATVDQMPK
                     HVSSGLMFGHPDLIPVPDLDRTSYLLMLGANPYESNGSLATAPDWPGRLQAIRERGGK
                     VVVVDPRRTRTAEVADEHVFIRPGADALFLFALANVLFEEGLVDPGDLTAHVAGIDEL
                     AVAVEPFTPELVAEATGVDPGRARRVARELAGADSAAVYGRIGTHTAEFGTMASWMVD
                     VLNVLTGNLDRPGGAMFSRAAHEQPRAPRGFQLGRWRSRVKELPEVRGELPVSTMADE
                     ILEPGPGQIRALVTIAGNPILSTPSSDRLDAALSGLDFMVSVDIYLNETSRHADVILP
                     GVSPLRRPHYDFAFNQLAVRNIANYSPPLFPLPDGTPDEWQVLLRLGAIVSGQGQDAN
                     LDALDDGVFGLMVGSAVGNPMSSIHGRDPEEIFVATTGGRGPERMLDFLVRTGPYGDG
                     YGADPDGLNLERLKTAPHGIDLGPLHSRLPADLCTPSGMVELAPEPLLADVPRLLDSA
                     GRAPNGGFLLIGRRHVRSNNSWMHNIDVLVKGKERCTLQISDVDAQRLGLGSDARAKV
                     SSSAGTLTVPIEITDSIMPGVVSLPHGWGHDLEGSALSVAAERPGVNTNRLSTGAMDP
                     VSGNAVLNGIPVEVVPA"
BASE COUNT          406 a          755 c          791 g          384 t
ORIGIN      
        1 ccctgccata ccttcgagat ctgctccagt tgttcacgat cccggatgag gacgaaatcc
       61 catctctgat tgttcctgga ggagggagcc ctccgccccg cctcgaggac cctctccagg
      121 tcctcgtcgg tcagcggtcg gtcgtcgtat tcccttgtgg cgcgtcgcgc cgtgatcgca
      181 tcccatgtct ccatggcccc aacctaacgc cccgacgagt gcccgaatcc ggcctcaggc
      241 cgggaccacc tcgaccggga tcccgttgag gacggcgttg cccgaaacgg ggtccatggc
      301 gccggtggag aggcggttgg tgttgacccc ggggcgctcg gcagccaccg agagcgccga
      361 gccttcgagg tcgtggcccc atccgtgggg cagactgacc acaccgggca tgatcgaatc
      421 cgtgatctcg atgggaacgg tgagcgtccc tgccgatgac gacaccttgg cccgggcatc
      481 tgagccgaga ccgagacgct gggcgtccac gtcgctgatc tggagggtgc agcgctcctt
      541 ccccttgacc agcacgtcga tgttgtgcat ccacgagttg ttggaccgga catgcctgcg
      601 cccgatgagg agaaagccgc cattgggggc tcggcctgct gagtcgagca gcctgggcac
      661 gtcggccagc aatggctccg gagccagctc gaccattccc gaaggtgtgc acaggtccgc
      721 gggcaggcgg gagtgaagcg gcccgaggtc gatcccatgc ggggcggtct tcaaccgttc
      781 caggttcaac ccgtccggat cggcaccgta tccgtctcca tacgggccgg ttcgcaccag
      841 gaagtcgagc atccgctccg ggccgcgacc accggtcgtc gcgacgaaga tctcctcggg
      901 atcgcggcca tggatcgacg acatcgggtt gccgaccgcc gagccgacca tcaacccgaa
      961 gacgccatcg tccaaagcat cgaggttggc atcctggccc tgcccggaca cgatcgcccc
     1021 gagtcgaagc agcacctgcc actcgtcagg ggtgccgtcg ggcagtggga acagcggtgg
     1081 tgagtagttg gcgatattgc gcaccgcgag ctggttgaag gcgaagtcgt aatgaggccg
     1141 ccggaggggg gaaacgccag ggaggatcac gtcggcgtga cgggaggtct cgttcaggta
     1201 gatgtcgacc gacaccatga agtcgaggcc ggagagagcc gcgtcgaggc ggtcgctgga
     1261 aggcgtggag aggatcgggt tcccggcgat ggtgacgagg gcgcgaatct gcccgggccc
     1321 cggctcgagg atctcgtcgg ccatcgtcga caccgggagc tctcccctga cctcgggaag
     1381 ctccttgacc cggctccgcc atcttccgag ctggaatccc ctcggcgccc tgggctgctc
     1441 gtgggcggct cgggagaaca tggcgccgcc ggggcggtcc aggttcccgg tgaggacatt
     1501 gaggacgtcg accatccagg aggccatcgt tccgaactcg gcggtgtgcg tcccgatccg
     1561 accgtagacc gcggccgagt cggcgccggc cagctctctc gccacccgtc gggcacgacc
     1621 cgggtcgacc ccggtcgcct cggcgacaag ctccggggtg aagggctcga cggccaccgc
     1681 caactcgtcg atgccggcga catgggccgt gaggtcgccc ggatccacca gaccttcctc
     1741 gaagaggaca ttggccaggg caaagaggaa tagtgcatcg gcgccggggc gaatgaagac
     1801 atgctcgtcg gcgacttcgg cagtccgggt gcgccgtggg tcgaccacga ccacctttcc
     1861 tcctcgctcc cgaatcgcct ggagccgacc gggccagtcg ggagcggttg ccagactccc
     1921 gttcgactca tacgggttgg cgccaagcat gaggaggtag gacgtccggt cgaggtcggg
     1981 gaccgggatc agatccggat ggccgaacat gagaccggag gagacatgct tcggcatctg
     2041 gtcgacggtg gccgcgctga agatgctctt cgtccccagc atcttgagaa agacccgggg
     2101 gtagagagtg cccgacatcg aatggacatt gggattcccc agataaacgc ctaccgagct
     2161 gttcccgtgg tcggcgatga tcggcaggag gcgagattcg atagcggtga acgcctcctc
     2221 ccaagacgcc ggctcgtgcc gtccatccca tttgatcagc gggacgcgca cccgatccgg
     2281 gtcttcgtcg atcttgccca gggtcgagcc cttgggacag atgaaaccat gactga
//
LOCUS       DASTXO010000520         5263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1800022, whole genome shotgun sequence.
ACCESSION   DASTXO010000520 DASTXO010000000
VERSION     DASTXO010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1800022"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /gene="uppS"
                     /locus_tag="VFU96_11020"
     CDS             <1..412
                     /gene="uppS"
                     /locus_tag="VFU96_11020"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEU5113839.1"
                     /translation="DRNRKHMAEAAEMTSANTSLNLVFAFNYGSRREIVDATRSLASR
                     VATGALAAEDIDEEMLAGSLYIADMPDVDLIIRSSGEHRLSNFLLWQAAYAEFYFPGI
                     LWPDFDRQALLDAVIDYQGRERRFGAAEDLSQID"
     gene            446..1753
                     /locus_tag="VFU96_11025"
     CDS             446..1753
                     /locus_tag="VFU96_11025"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEU5113840.1"
                     /translation="MTQPSLEAISNLAKKRGFVFQASEIYGGIRSAYDYGPLGVELLR
                     NVKEQWWRDMVQLRDDVVGIDSAILQARRVWQASGHEEVFTDPLVECRNCNARHRLDK
                     LDDPNTCPTCGKSGTFTEPKPFHLMFRTHMGPVDSDENLIYLRPETAQGIFTNFENIR
                     RTNRLKLPFGIAQVGKSFRNEITPGQFVFRTREFEQMEMEYFVRPEEANEWFSYWQEQ
                     RFNWYVGLGMTESNLRIRPHDEDELSHYSTGTVDIEYRFPWDWDELEGIANRADYDLK
                     QHSEYSGEDLRYFDPDTNERFFPYAIEPAAGATRTTFAFLIDAYHEETVNDETRTVLR
                     LDPRLAPYKAAILPLSKKEELIEPAERIAIELRQRWMVETDVTQSIGRRYRRQDEIGT
                     PYAVTVDFETLEDRAVTVRDRDSMKQDRVSMDRLVDYLAERLG"
     gene            1828..3564
                     /gene="dnaG"
                     /locus_tag="VFU96_11030"
     CDS             1828..3564
                     /gene="dnaG"
                     /locus_tag="VFU96_11030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU5113841.1"
                     /translation="MAERGDIDRVRESTDLVDLISEVTKVKRSGRSFMAVCPFHEEKT
                     PSMSVDRARGLYHCFGCGKGGDVFSFVQETQGIDFADALELLARRAGITLVRDAADAR
                     QRGRRGEAVEALKKAIDVYHDRLKKSPEAGPARAYLRQRGYDVGIIDEWRLGFAATDW
                     DTLTKELRAGGLDDRVLVDAGLSRRGRHGLFDVFRGRLMFPIHDLRGDAVGFGGRKID
                     EIDRNATNNPDAKYVNSADSVVYHKAQVLFGLDRARREISDDHPAVIVEGYTDVIAMH
                     QAGVKTAVATCGTALGDGHFDLLRRFSEKVVLAFDSDEAGSRAALRGDELESPFRLDL
                     DLRVAVMPDGLDPADLVQQERNDELVAAVNEARPLLERRIEHEVSRHDLSGPEGRARA
                     LHAAAAQVRRVNDAIARREYARFVARLVGVDLETAESAIEGERAGRGRVETGGERPLD
                     RIESELLRVLIADPASVEGVVADDFTDERLRQAFSALDLSETRAGEPADISRAGDEGA
                     RSLLRSLAMDDRPLASGPEILARVKERRLDVEIEDLQRKLVGMEPGTDAHSDNLRRLI
                     ALQQEKRSSTRM"
     gene            3561..4688
                     /locus_tag="VFU96_11035"
     CDS             3561..4688
                     /locus_tag="VFU96_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5113842.1"
                     /translation="MNEELSKVVAALVKRGKHRGFLTMAEVQQELEDAEASGEAFEEV
                     ADALRSQGIKLTEEGPGVTEEFIEVGQISVSDPVRLYLNEIGRYPLLTSQQEVELAMQ
                     MEAGHRAQARLAEEGLIDDDRAFLGHEIDMGDAAQKQLVQSNLRLVVALARRYVGRGM
                     ALLDLIQEGNVGLMRAVERFDYRRGFKFSTYATWWIRQAISRAIADQGRTIRMPIHVL
                     DSVNKLTRAQREMTQAMGRAPTIDELAFDLDIEPARVAELQRIAQDTVSLETPVGEDE
                     DGTLGDLVEDLESDRPPDVATFSSLQDQLAMALEGLNEREREVLIMRFGLADGRMLTL
                     EEVGSHFKVTRERIRQLETKALAKLRHPDKSHKLEGFLENS"
     gene            4732..4977
                     /locus_tag="VFU96_11040"
     CDS             4732..4977
                     /locus_tag="VFU96_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004001483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU5113843.1"
                     /translation="MRFRAGLAVGAAVGYVLGARAGRQRYEQIKRWAGEARKHPAVAQ
                     LANQSIGVVDAGRYIAATGLSAGAKGLRTIDSQAGSE"
     gene            5020..5092
                     /locus_tag="VFU96_11045"
     tRNA            5020..5092
                     /locus_tag="VFU96_11045"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5053..5055,aa:Asn,seq:gtt)
BASE COUNT          997 a         1580 c         1740 g          946 t
ORIGIN      
        1 ggatcggaat cgcaagcaca tggcagaggc ggccgagatg acgtcggcca acacgagtct
       61 caatctggtg ttcgccttca actatgggag ccggcgcgag atcgtcgatg cgaccagatc
      121 cctcgcctcc cgggtggcga ccggggcgct ggcggcagag gacatcgacg aggagatgct
      181 ggccgggtcg ctgtacatcg cggacatgcc cgatgtcgac ctgatcattc gatcttcggg
      241 agagcatcgg ctctccaatt ttctcctctg gcaggcggca tacgccgagt tctactttcc
      301 cgggatcctg tggcccgact tcgaccggca ggcgttgctg gacgcggtga tcgactacca
      361 gggccgggag cgtcgtttcg gcgccgcgga agacttgtcc cagattgact aagcggccgg
      421 atcgggccac cgttcccaaa ctcgtatgac gcaaccatcc ctcgaggcca tctccaatct
      481 cgccaagaag cggggctttg tctttcaagc ctccgagatc tacggcggaa tccgctccgc
      541 ctacgactac gggccgctcg gcgtggagct gctgcggaac gtcaaggagc agtggtggcg
      601 ggacatggtc caactgcggg acgatgtggt cgggatcgac tcggcgatcc tccaggctcg
      661 tcgggtttgg caggcgtctg gtcacgagga ggtgttcacc gatccactgg tcgaatgtcg
      721 caattgcaat gcccggcatc gtctcgacaa gctcgatgac ccgaacacct gcccaacctg
      781 cgggaagtca gggacgttca ccgagcccaa gccgtttcat ctcatgtttc gcacccacat
      841 ggggccggtc gactccgacg agaacctgat ctatctcagg ccggagacgg ctcaggggat
      901 cttcacgaac ttcgagaaca tccgccgcac caatcgtctc aaactcccgt tcgggatcgc
      961 ccaggtgggc aaatcctttc gcaacgagat caccccgggg cagttcgtct ttcgcacgcg
     1021 cgagttcgaa cagatggaaa tggagtactt cgtgcggccc gaggaagcga acgaatggtt
     1081 ctcgtactgg caagagcagc gattcaactg gtatgtcggt ttgggcatga ctgagtcgaa
     1141 tctccggatt cggccccacg acgaagacga gctcagccac tattcgacgg gcacggtcga
     1201 catcgagtat cggtttccct gggattggga cgagctcgag gggatcgcaa accgcgccga
     1261 ctacgacctg aagcaacatt cggagtactc cggtgaagat ctccgctatt tcgaccccga
     1321 cacgaacgag cggttcttcc catacgccat cgaaccggcg gctggggcga cccggaccac
     1381 cttcgccttc ctcatcgacg cttatcacga ggagacggtt aacgacgaga cgaggacggt
     1441 cctccgcctc gatccccggc tcgctcccta caaagcggcc atccttccct tgtcgaagaa
     1501 ggaggagttg atcgagcctg ccgagcgcat cgccatcgag cttcgtcaac ggtggatggt
     1561 ggagacggac gtgacccagt cgatcgggcg gcggtatcgg agacaggacg agatcgggac
     1621 tccgtatgcg gtgacggtcg acttcgagac attggaggac cgagcggtca cggtgagaga
     1681 tcgggactcc atgaaacagg atcgtgtctc gatggatcgc cttgtcgact atctcgccga
     1741 gcgtctcggc tgacggcatt ctcgcctgtc gccaaatcgt cgccgcatcg tgaagcagcc
     1801 gccggtcgta cttatgatcg ccggaccatg gcagagcgag gtgacatcga tcgggttcgg
     1861 gaatccaccg acctcgtcga cctgatctcc gaggtcacca aggtcaagcg atcgggccgc
     1921 agcttcatgg cggtttgccc gttccacgag gagaagacgc cgtcgatgtc cgtggatcgg
     1981 gctcgtgggc tctatcactg cttcgggtgt gggaagggcg gtgacgtatt cagcttcgtg
     2041 caggagacac aaggaatcga cttcgccgat gccctcgaac tcctcgcccg gcgcgccggc
     2101 atcaccttgg ttcgtgacgc ggccgacgct cgtcagcggg gacggcgggg cgaggcggtg
     2161 gaggcgctga agaaggcgat cgacgtctat cacgatcgtc tgaagaagtc tccagaagcc
     2221 gggccggccc gggcctacct ccgccagcgt ggatacgacg tcgggatcat cgacgaatgg
     2281 aggctcgggt tcgccgccac cgactgggac acgctgacca aggagttgag ggccggaggg
     2341 ctggatgacc gggtcctcgt cgatgccggt ctgtcccggc ggggccgaca cgggctgttc
     2401 gacgtgttca ggggccggct catgttccca atccacgatc tccgtggcga cgcagtcgga
     2461 ttcggtggcc gcaagatcga cgagatcgat cggaatgcga cgaacaaccc cgacgccaag
     2521 tacgtcaact ctgccgactc tgtggtctat cacaaggccc aggtcctctt cgggctcgac
     2581 cgtgcccgac gtgagatatc cgatgaccat ccggcggtga tcgtcgaggg gtacaccgat
     2641 gtcatcgcca tgcaccaggc gggcgtcaag acggctgtgg cgacctgtgg cacggcactc
     2701 ggggacggcc atttcgatct cttgaggcga ttcagcgaga aggtggtctt ggcgttcgac
     2761 tccgatgagg caggctccag agcggccctc cgcggcgacg agctggaatc gccgttccgt
     2821 ctcgacctcg atctgcgggt cgccgttatg cccgatggcc tcgacccggc cgatttggtc
     2881 cagcaggagc gcaacgacga gctcgtggca gcggtcaacg aggcgagacc tctcctggag
     2941 aggagaatcg agcacgaggt gagtcggcac gacctgtccg gtcctgaggg ccgggcccgt
     3001 gctcttcatg ccgccgcggc ccaggtacgc cgggtcaacg atgccattgc tcgccgggag
     3061 tacgcccggt tcgtggcccg gctcgttggc gtcgatctcg agacggcgga gtcagcgatc
     3121 gaaggcgagc gggccgggcg cggtcgagtc gagaccggag gggagcggcc cctcgatcga
     3181 atcgagtccg agctgcttcg ggtgctgatc gccgacccgg cgtcggtgga aggtgtggtg
     3241 gccgacgatt tcaccgatga gcgtctgagg caggcgttct ccgctctcga cctttcggag
     3301 acacgagccg gtgagccggc tgacatctcc cgagccggtg atgagggggc acgatcattg
     3361 cttcgctcgc tggccatgga cgaccgtccc cttgcgtcgg gacccgagat cttggcccgg
     3421 gtgaaagaac gacgactgga cgttgagatc gaggatctgc agcggaaact ggtcgggatg
     3481 gagccgggaa ccgatgctca ttccgacaac cttcgccgct tgatagcttt acagcaggag
     3541 aagcgatcct ccacccggat gtgaacgagg aactcagcaa ggtcgtcgcc gccctggtca
     3601 agcggggcaa gcatcgtggc tttctcacga tggcagaagt ccagcaggag ctggaggatg
     3661 ccgaggcgtc cggggaggca ttcgaggagg tcgccgacgc gctccgctcc cagggaatca
     3721 agctgacgga ggaagggccg ggcgtcaccg aggaattcat cgaggtcggc cagatctccg
     3781 tgtcggaccc ggttcgcctc tatctgaacg agatcggccg gtacccgctg ctcaccagcc
     3841 agcaggaggt ggaactggcg atgcagatgg aggcgggaca tcgggcacag gcccggctcg
     3901 ccgaggaggg cctcatcgat gatgaccggg ccttcctcgg acacgagatc gacatgggtg
     3961 atgccgccca gaagcagctg gttcagtcca atcttcgact ggtcgtcgcg ttggcccgcc
     4021 gttacgtggg ccggggtatg gcgctgctcg atctgatcca ggagggcaac gtcgggctca
     4081 tgcgcgccgt cgagcggttc gactaccgcc ggggcttcaa gttctcgact tacgcaactt
     4141 ggtggatccg ccaagcgatc tccagggcga tcgccgatca gggcaggacg attcgcatgc
     4201 cgatccacgt cctcgactcg gtaaacaagc tgacgcgggc ccagagggag atgacccagg
     4261 cgatgggccg ggcgccgacc atcgatgagc tggcgttcga tctcgacatc gagccggccc
     4321 gggtcgcgga gctgcagcga atcgctcagg acacagtctc gttggagacg cccgtaggcg
     4381 aggacgagga cgggacactc ggtgatctcg tggaggatct cgagtccgat cgcccgcccg
     4441 acgtcgccac tttctcttct ttacaggacc agttggccat ggctctcgaa ggcctgaacg
     4501 agcgggaacg agaggtcctg atcatgcggt ttggtctcgc cgacgggagg atgctcaccc
     4561 tcgaagaggt cgggtcgcat ttcaaggtga cgcgggagcg gatacgacag ctcgagacaa
     4621 aggcgctggc caagctccgc catcccgaca aatcgcacaa gctggaaggg ttcctggaga
     4681 actcctgatc gagctggaca cagggtgtgc tcgccgtggc acaatccgtg gatgagattc
     4741 agagccgggc tcgcggtcgg tgccgcggtt ggttatgtcc tcggtgcccg ggccggccgg
     4801 cagagatacg agcagatcaa acgctgggcc ggtgaagcca gaaagcatcc tgcagtggct
     4861 caattggcga atcagagcat cggcgtagtc gatgccggcc gatatatcgc tgcgacgggg
     4921 ctctcagcgg gggctaaggg tttgcgcacg atcgacagtc aggccggctc cgagtagcca
     4981 gaggccgact ggtaccctga ccctgccagt cggcaacctt ccgcggtagc tcaattggta
     5041 gagcagtgga ctgttaatcc attggttgta ggttcgagtc ctacccgcgg agcagagctt
     5101 cctcccgtat tcattcgatt ctctcgctcc tggcccactc agccgaagga ggaccaacac
     5161 aagggagtga cgtcctgtga cgcgcttcat tgaagattcg acgcagggtc acgtcaaccg
     5221 agtgcgcgtc ggcgtcgagt cgccggaacc tcggaacgac tgc
//
LOCUS       DASTXO010000521         2101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_290069, whole genome shotgun sequence.
ACCESSION   DASTXO010000521 DASTXO010000000
VERSION     DASTXO010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290069"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VFU96_11050"
     CDS             <1..537
                     /locus_tag="VFU96_11050"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEU5113844.1"
                     /translation="LVEGGLFAVGTVGQLYDTTLGALADHGFGVSDARMTSNRARETS
                     ALAGEKGNLVALGDVAGVFRSLHEAGIRLAIVSSDDRSAIDSAVDALGVGTMLDAVVS
                     GDEGLDAKPAPAALIEAARRLGLEPRRLLYVGDSWVDAEAGRAAGVAGVVLVGEQSAE
                     NSLLATVVMPDIASLQVG"
     gene            complement(555..1385)
                     /locus_tag="VFU96_11055"
     CDS             complement(555..1385)
                     /locus_tag="VFU96_11055"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU5113845.1"
                     /translation="MTVFALTLALLAAVMHAAWNLLVKSSEDQLVAGWTVVAGAAILA
                     LPLLVFSGLPDRSVWDLILISTLVQVFYVLTLAAAYRVGDLSFVYPLARGTSPVLVSL
                     AGVAFLGDEISPLGWLGVLTVTAALTLLAWRRPDRSGLGAALATGVLISAYTSVDAAA
                     VREQGSAVSVIAAEFVLLSIALGLVVLSLRGPRAMLGMVRADWRKAAMGGFATGVAYL
                     LVMVAALSAPVGLVSGVRETSVLIAVVASRLVLHEEVTSYQLGMVAAAVAGIGLIAIG
                     "
     gene            1702..1920
                     /locus_tag="VFU96_11060"
     CDS             1702..1920
                     /locus_tag="VFU96_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113846.1"
                     /translation="MVSSIAIGLGMPAWSGGSEPEEMRGLSVFALRGVVAAAFTTQRE
                     PVAAPIEPHHSQRHPKPPDQTFPIPFIR"
     gene            complement(1910..>2101)
                     /locus_tag="VFU96_11065"
     CDS             complement(1910..>2101)
                     /locus_tag="VFU96_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113847.1"
                     /translation="PDDEGKAGRVIWNFEKFLLSPSDEVRRFRPTTEPDAPEVISAIE
                     SYLPKPSGSADRAADVSTG"
BASE COUNT          405 a          643 c          702 g          351 t
ORIGIN      
        1 ctcgtggaag gcgggctctt cgccgtcggc acagtcgggc agttgtacga cacgacgctg
       61 ggagcgctgg ccgatcatgg tttcggcgtt tccgacgccc ggatgacatc caaccgcgcc
      121 agggagacat ctgcattggc cggggagaag ggcaatctcg tggccctggg ggatgtggca
      181 ggtgtcttca gatcgctcca tgaggcaggt atccggctgg cgatcgtgtc gagtgatgac
      241 cgcagcgcaa tcgattcagc ggtcgatgcc ctcggcgtcg ggacgatgct cgatgccgtt
      301 gtgtccggtg acgaaggtct cgatgccaaa ccggccccgg ccgcgctgat cgaggccgct
      361 cgccgacttg gcctcgaacc gcggcggctg ctctatgtcg gcgactcgtg ggtcgacgcc
      421 gaggcggggc gggcagcggg ggtggccggg gtggtgctgg tcggggagca gtcggcggag
      481 aactcgttgc tggccacggt ggtaatgcca gacatcgctt ctctccaggt tggatgacat
      541 atccggggtc gaaatcatcc gatggcgatg agcccgatgc cggcgacggc ggcggcgacc
      601 atcccgagct gataactggt gacctcctcg tgaagtacaa gtctcgaggc gaccaccgct
      661 atcaacactg aagtctccct cacacccgat accaatccga ccggcgcgct cagggcggca
      721 accatcacga gcaagtaggc cacaccggtc gcaaatcctc ccatcgcggc ttttcgccaa
      781 tccgccctga ccatcccgag catggccctg gggccacgca aggagagcac gaccagtcca
      841 agagcgatcg acagcagaac gaactcggcg gcgatgaccg acactgctga gccctgctcc
      901 cgaacggcgg cggcatcgac cgaggtatat gcagagatca acacgcctgt cgccagagcc
      961 gcaccgagcc ccgatcgatc cgggcgccgc catgcgagca aggtgagcgc cgcggtcacg
     1021 gtgagcacac ccaaccaccc caggggcgag atctcgtcgc cgaggaatgc gactccggcg
     1081 agtgagacga gcacggggga ggttccgcga gcgagggggt agacgaagga caggtcaccg
     1141 acccggtacg ccgccgccag cgtcagcacg tagaacacct gaacgagggt cgaaatgagg
     1201 atcaggtccc aaaccgaccg atcggggagc cccgagaaca ccagaagagg aagggcgaga
     1261 atggcggcgc cggccaccac ggtccacccc gccacgagct ggtcctcact cgatttgacc
     1321 agcaaattcc acgccgcgtg catgactgca gcgaggaggg cgagggtcag agcgaaaacg
     1381 gtcatgcgga tttgttcgtc gcagaaggct cgaggcctgc caacaccacg acgatttcag
     1441 catccggaag cgagatttcc ttgattcatc gtcctgtaac ccaaataccc cgggacgttc
     1501 agggtgggct tctaggttgt cggcgcagcc gaaaggagcc ctgtggaagt ctggagacgt
     1561 tcgaagtggg tcctgctgat ggtgctggcc tcggtgctct tcctggcgtc gatcttgggg
     1621 ccgtccatac cgatctgatt ggtcgaaatc ggggacccgg ctggctcaag tccggacgga
     1681 gattgccgat gatcaatcgg gatggtttct tcgattgcca tcgggttggg aatgccggca
     1741 tggtctgggg gcagcgagcc tgaggagatg cgcggcttga gcgtgttcgc cctacgcggc
     1801 gtcgttgcgg cagccttcac tacgcagcgg gagccggtgg cggcgccaat cgagccacat
     1861 cattcccagc gacacccgaa gccgcccgat cagacgttcc cgatcccgtt catccggtag
     1921 atacgtctgc agctcgatct gctgacccgg acggcttggg caggtaactc tcgatggcgc
     1981 tgatcacctc tggagcatcg ggctcggtcg tgggccggaa tcgacggact tcgtcggaag
     2041 gggataggag gaacttctcg aagttccaga tcacgcggcc cgccttgccc tcgtcatcag
     2101 g
//
LOCUS       DASTXO010000522         4673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1099754, whole genome shotgun sequence.
ACCESSION   DASTXO010000522 DASTXO010000000
VERSION     DASTXO010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099754"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VFU96_11070"
     CDS             complement(<1..549)
                     /locus_tag="VFU96_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113848.1"
                     /translation="MVDQVIYDRGYRSYDGPRLGPKGARRAVYKDGVRRVLGLGRKAR
                     QKIFPWSLIVIAIVAAAVFVGIHWAIGNIEESLRQEVPTYGGLFDFYSGISLLFIALA
                     GPQLLIPDRTRGVLSVYFSRPLTVDGYLTSKVGAFATVVGAIYILPQVVLHLGLALIA
                     EEGFLSYLGNNLDILWKVPVTAV"
     gene            complement(549..1451)
                     /locus_tag="VFU96_11075"
     CDS             complement(549..1451)
                     /locus_tag="VFU96_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019875424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5113849.1"
                     /translation="MSAILEVDDLTHDYGEVVALSGLTLEVPPGVTGLVGANGAGKTT
                     MLRLMLGLLHPTSGSMQVLGHDPQREPLVVRSRIGYMPEGECLPKDQTAADFVSYAAQ
                     LAGIPSHEAKRRSSETLFLVGLEEERFRFLGDFSTGMQQRVKLAQAIVHDPDLLLLDE
                     PASGLDPEGRTQMLDLIKRLSDFDVNVIVSSHVLTDIEQTCDWVVMLDAGNLLRSGPL
                     QGFEKLGTVLVEVLEHPEEVAATLRAQGWEVAVDGLRLVVGPGDETIEPAVISAVAET
                     GSGLVRMIRGSASLEDLFLNRETV"
     gene            complement(1448..2467)
                     /locus_tag="VFU96_11080"
     CDS             complement(1448..2467)
                     /locus_tag="VFU96_11080"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014694015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEU5113850.1"
                     /translation="MSHPFEPPIETMEARTSEQWPRGDYLYEPKWDGFRSISWSEPEN
                     RIDSRNQRPLLRYFPELTPALDQLPAGTVVDGEIVVVIDGVTDFDSLQQRIHPAVSRV
                     TMLSEQTPAELIAFDLLADRGEDLRERPFTERRERLVELAGSLSDPWHLTPQTDSVEL
                     ARDWFDEFESAGCDGIVAKVPDLGYQHGKRAMIKIKHRRTIDCVVGGFREHKDGGKIG
                     SLLLGLYDDRGELHFIGHCSGFGDADRVEIFDRFKDLAVDGSFGEEARAPGAQSRWSA
                     GKTLDWTPVEAGVVVEVSYDQLEGDRFRHATRFHRWRPDKDPLQCTMEQLERPEGPGF
                     REVVS"
     gene            2506..3507
                     /locus_tag="VFU96_11085"
     CDS             2506..3507
                     /locus_tag="VFU96_11085"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU5113851.1"
                     /translation="MEKYITRLPSWARGPAEILAGAAEGYGAHRASRMAAAISYRTIF
                     ALAPLLVIAVAVLGSFLGSQAEAQQEIIEGIEAVAGPEIAAIVEDILTTALTSTGTAY
                     LIGGILLIWTASSLFLEMQRDLNDIFEVPQEEVAGIVAMVRQRGIGFLWVLGLGFLLV
                     ATWLINALWGFVSNLLPGYFDVVDDMVSVITPLASLLLLPLVFALIFQTMTTVRIPWR
                     AAWAGGAFTAVVFLIAAYGVGIYFDIAGATTALGFAGSFVVILFLAYFLSMVFLYGAE
                     VTKVYSDRLASREAPPVSRPLYSDPQVVVAEPPAGIPQAAFLAFVAGLIAGWRRSRR"
     gene            3549..4115
                     /locus_tag="VFU96_11090"
     CDS             3549..4115
                     /locus_tag="VFU96_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113852.1"
                     /translation="MSVDIAGFVTDLKEHATEHGFHVHDERHFVETYSLRQSWEVDLH
                     PEAGCGGPIDLHVALDVDPKVILSLREALDEMDSDFEEPENVYHLDLFFNWAVPPLHS
                     PPDLLVLATDLAGLAGVTLPVEVSAIDSYGAVTDAPERRLTLTGRASVSLVDVLMGRD
                     QLCDVLDRSHEVSEYLLDRVGGWMELPS"
     gene            complement(4112..4498)
                     /locus_tag="VFU96_11095"
     CDS             complement(4112..4498)
                     /locus_tag="VFU96_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113853.1"
                     /translation="MTTFPGVATLKEDNVPVMVGLDDEKITLVSGEISIAEWMAGQYE
                     VIDLGSGEFVIEAEDDSIPFLPDDPSGFALGIRKETEAPIPGDRSSTTLEVREGPPPK
                     PATVIAFWVLAVFTAALGIWATLSLF"
BASE COUNT          806 a         1508 c         1493 g          866 t
ORIGIN      
        1 gacggcggtc acggggacct tccacaggat atcgaggttg ttgcccagat aggagagaaa
       61 gccctcttcg gcgatgaggg cgaggccgag gtggaggacc acctggggaa ggatgtagat
      121 ggccccgacg accgtggcga aggcaccgac cttggaggtg aggtaaccat ccactgtcag
      181 cggtcgggag aagtacaccg agaggactcc tctggtccgg tcggggatga ggagctgggg
      241 gccggccagg gcgatgaaca gcagggagat gcccgagtag aagtcgaaga ggccaccgta
      301 ggtggggacc tcctgacgga gactttcctc gatgttgccg atcgcccaat gaatgccgac
      361 gaaaacggcg gcggcgacga tggcgatgac gatgagcgac caggggaaga tcttttgccg
      421 cgccttgcgc cccaggccca ggacacgacg cactccgtcc ttgtagaccg cccttctcgc
      481 ccccttcggg ccaagacggg gcccgtcgta cgaccgataa ccgcggtcat agatgacctg
      541 gtcgaccact agacggtctc ccggttgagg aacaggtcct cgagcgatgc cgatcctcgg
      601 atcatccgca ccaaaccgga gccggtctcg gctaccgcgg agatgaccgc gggttcgatc
      661 gtttcgtcgc ccgggccaac gaccagccgc agtccgtcga ccgcgacttc ccaaccctgg
      721 gctcgcagcg tggctgcaac ctcctccggg tgctccagca cctcgacgag gacggtgccc
      781 agcttctcga agccctgaag ggggccggac cgaaggagat tcccggcatc gagcatgacc
      841 acccagtcgc aggtctgctc gatgtcggtc aggacgtgtg aggacacgat gacgttgacg
      901 tcgaagtcgg aaagcctctt tatcaagtcc agcatctggg tgcggccctc cgggtcgagc
      961 ccggaggcgg gctcgtcgag gaggaggaga tccgggtcat ggacgatggc ctgtgccagc
     1021 ttgacccgct gctgcatgcc ggtggagaag tcgccgagga agcgaaagcg ctcctcctcg
     1081 agcccgacga ggaacagcgt ctccgacgat cgcctcttcg cctcatgcga cggtatgccg
     1141 gcgagctggg cggcgtagga gacgaaatcc gctgcggtct gatctttggg cagacattcg
     1201 ccttcgggca tgtagccgat ccgcgatcgc accaccagcg gctcccgctg cgggtcatga
     1261 cccaggacct gcatcgatcc cgaggtgggg tggaggagcc cgagcatgag ccggagcatc
     1321 gtggtcttgc ccgcaccatt cgccccgacc aggccggtca ctccgggggg cacctcgagg
     1381 gtgagcccgc tgagggcaac gacctcgccg tagtcgtggg tgaggtcatc gacttcgagg
     1441 atcgcgctca actcaccacc tcgcggaatc cggggccttc cggacgctcg agttgttcca
     1501 tcgtgcactg aagagggtcc ttgtcgggcc gccagcgatg aaagcgggtt gcatgcctga
     1561 accggtcacc ctccagctgg tcgtagctga cctcgaccac aaccccggcc tccacaggtg
     1621 tccaatcgag cgtcttcccg gccgaccacc ggctctgcgc tcccggggct cttgcctcct
     1681 ccccgaagct gccgtcgacc gcgaggtcct tgaaccgatc gaagatctcg acccgatcgg
     1741 catcgccaaa cccggagcag tgaccgatga aatggagctc accccgatcg tcgtagaggc
     1801 cgaggaggag ggagccgatc ttccccccgt ccttgtgctc gcggaagccg ccgacgacgc
     1861 aatcgatggt tctgcggtgc ttgatcttga tcatcgcccg cttcccgtgc tgatagccga
     1921 ggtcggggac tttggcgacg atgccgtcgc accctgccga ctcgaactcg tcgaaccagt
     1981 ctcgggctaa ctccacggag tcggtctgcg gggtcagatg ccatgggtcg gacagggagc
     2041 cggccagttc cacgagacgc tcccgacgtt cggtgaatgg gcgctcccga aggtcttccc
     2101 cacggtcggc aagcaggtcg aaggcgatca attcagccgg ggtctgttcc gagagcatcg
     2161 tgactcggga caccgccggg tggattctct gctggagcga gtcgaagtcg gtcacaccgt
     2221 cgatcacgac cacgatctcg ccgtcgacga cggtgccggc cggcagctga tcgagagcgg
     2281 gtgtcaactc gggaaagtag cggagaagtg gacgttggtt tcgggagtcg atccggttct
     2341 ccggctccga ccacgagatc gagcgaaacc cgtcccactt cggctcgtag aggtagtccc
     2401 ctcttggcca ctgctctgat gtccttgcct ccatcgtctc gatgggtggc tcgaagggat
     2461 gactcaccgg ggccgagtgt aagcagcgat agcctgctcc ccgagatgga gaagtacatc
     2521 acacgtcttc catcgtgggc acgggggccg gccgagatcc tggccggcgc ggccgagggg
     2581 tacggcgctc atcgggcctc gcgcatggcg gcggccatct cctaccgcac catatttgcc
     2641 ttggctccgc tcctggtcat cgccgtcgcc gtgctcggga gcttcctcgg cagccaggcg
     2701 gaggcgcagc aggagatcat cgaggggatc gaggcggtgg ccgggccgga gatagcggcg
     2761 attgtcgagg acatcctgac cactgctctc acctccactg ggaccgccta cctgatcggg
     2821 ggaattctcc tcatctggac cgcctccagc ctctttctcg agatgcaacg cgacctcaac
     2881 gacatattcg aggtgccgca agaggaggtc gccgggatcg tggccatggt ccgccagcgc
     2941 gggatcgggt ttctctgggt tctcggtttg ggcttcctgt tggtggcgac atggctcatc
     3001 aacgccctgt ggggattcgt gagcaatctc cttccgggat acttcgacgt ggtcgacgac
     3061 atggtcagcg tcatcacacc tcttgcctcc cttctattgc tgccgctggt cttcgccctc
     3121 atctttcaga caatgaccac cgtgagaatc ccctggcgag cggcctgggc aggcggagcg
     3181 ttcaccgcgg tcgtttttct cattgccgct tacggcgtgg ggatctattt cgacatcgcc
     3241 ggggccacca ccgctctcgg cttcgccgga tcgttcgtgg tgatcttgtt cctggcctac
     3301 ttcctgtcga tggttttcct ctacggggcc gaggtgacca aggtctactc ggatcggctc
     3361 gcatcccggg aagccccacc cgtcagcaga cctttgtaca gcgaccctca ggtggtcgtg
     3421 gcggagccac cggccggcat cccccaagcc gccttcctgg cgttcgttgc aggtttgatc
     3481 gcggggtggc gccgaagccg ccgctgacct ctgtcaattg aaaccggact ccaataacct
     3541 cacgctcaat gtccgttgac atcgccgggt tcgtcaccga tctgaaggaa catgccaccg
     3601 agcacggctt ccacgtacac gatgagcgtc atttcgtcga gacctactcc ctgcgccagt
     3661 catgggaggt cgatctccat ccggaggcgg gatgtggggg gccgatcgac ctccatgtcg
     3721 ccctcgatgt cgaccccaag gtgatcctgt ccttgagaga ggccctcgac gagatggact
     3781 ccgacttcga agagcctgag aacgtctatc acctggatct gttcttcaac tgggcggtcc
     3841 cccctctcca cagcccgccg gatcttctcg tcctcgccac cgacctcgcc ggtctggccg
     3901 gcgtgacgct tcccgtcgag gtgtctgcca tcgactcgta cggggcggtg accgacgctc
     3961 ccgagcgcag gctgaccctg accgggaggg cgtcggtcag cctcgtggac gtgctcatgg
     4021 gccgggacca gctctgcgac gtgctcgatc gctcccacga ggtgagcgag tatctcctcg
     4081 accgtgtcgg cggctggatg gagctgccga gctagaacaa gctcagtgtt gcccagatcc
     4141 cgagggcggc ggtgaacaca gcgagcaccc agaaggcgat gacggtggcc ggcttcggtg
     4201 gtggcccttc cctgacctcg agtgtcgtcg acgaccgatc gcccggtatt ggggcctcgg
     4261 tctctttccg gatgccgagg gcgaagccgc tcgggtcatc ggggaggaag ggaatggagt
     4321 cgtcctcggc ctcgatcacg aactcgccgg aaccgaggtc gatgacctcg tattgcccgg
     4381 ccatccactc agcgatcgag atctccccgg agacgagcgt gatcttctcg tcgtccagcc
     4441 ccaccatgac cggaacattg tcttccttga gcgtcgcgac gcccgggaat gtcgtcatct
     4501 tcctcgtccc ccaggaaccc ttgtcaagct aggcgctgca cccggcttcg caatcctaga
     4561 agggcagccc gagtgcgtca cacaggaagc cggtgaagcg gcctgccttc gagaacatcg
     4621 tcgccaactc ctcgtcgaag ccggtgccgg tcacctgctt ctgggtgagg ctg
//
LOCUS       DASTXO010000523         7902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1321216, whole genome shotgun sequence.
ACCESSION   DASTXO010000523 DASTXO010000000
VERSION     DASTXO010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7902
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321216"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1269)
                     /locus_tag="VFU96_11100"
     CDS             complement(<1..1269)
                     /locus_tag="VFU96_11100"
                     /inference="COORDINATES: protein motif:HMM:NF024875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HEU5113854.1"
                     /translation="MILAEELSELAHRMGAAGFGVTGAETFSRERAALRASRESGRSG
                     PLRFTYDDPDVATDISLSFPWARSLVVVSYAYLQQAETPTDEGALVGRFATTNHYAGV
                     RRITDAIAGFLQAGGYRAETLCDDNRLVDRAAAVRAGVGWPGKSTMVLAPGHGPWMLL
                     GTVVTDASIPVSRPMERDCGSCSTCIPACPTGALGEWGLDARRCLSTWLQTPGSIPQW
                     IRPLIGRRIYGCDECLTACPPGGRALAAAGETPGNLRFGDLLGRSDGDLLDRYSWWYV
                     PRRQGRFIRRNLLVAAGNSGEPDAREVVEAHTRHASSMIRGHAYWAMARGFEGRSVLR
                     DALESETVPEARDELLLALLMTEHPGAHVAVLEADERSRDNDSIRALAIVGPHAVAEG
                     RGTDDLELVFVGRDGGEADQIEMPMVRVYDP"
     gene            1337..1918
                     /locus_tag="VFU96_11105"
     CDS             1337..1918
                     /locus_tag="VFU96_11105"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEU5113855.1"
                     /translation="MRAVRILYAFVVMTVDGFYEGPDGEFDWPNVDDEFDRYSVASLE
                     DTGLIVFGRVTYEGMASYWPTAAALEEDPIIAPLMNEIPKAVVSSTIENAAWNNSTVI
                     RGDVANEISKLKAQSGKSIGVFGSPNLTLSLIEMGLVDELRLMIHPVALGSGNSLFRT
                     MTGRLRFDLLGTHTFESGNVLLTYRPGVPTGLA"
     gene            complement(1967..2332)
                     /locus_tag="VFU96_11110"
     CDS             complement(1967..2332)
                     /locus_tag="VFU96_11110"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5113856.1"
                     /translation="MSKSSTTSETRILELESAFWESAGDPRFYRENFADDGVMALPDG
                     LMTKAQVISAMEEATPWSRFSIDDVRFVDVGDDVTALVYTADATRDGRDDGYRAAISS
                     VYVNRGGAWRLVLHQQTPL"
     gene            complement(2372..2725)
                     /locus_tag="VFU96_11115"
     CDS             complement(2372..2725)
                     /locus_tag="VFU96_11115"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HEU5113857.1"
                     /translation="MRRVFFLAIALTSMLAACSGESGDTTGANDGAGGEEQRVEIADL
                     AFNPETLTVAAGSTVTWVNADPDLPHTSTSDDELWDSGTLNEGGEFSFTFDEPGTFAY
                     FCEVHPTMRGSIVVE"
     gene            2866..3702
                     /locus_tag="VFU96_11120"
     CDS             2866..3702
                     /locus_tag="VFU96_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011603052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU5113858.1"
                     /translation="MPPDLHDRVTLSNGVAMPIIGYGTWPIPDREAENLVAVALDVGY
                     RLIDTAEMYRNEEGVGRGIRASGIDRDDVFVTTKLSQRWHGYEEAQQAFTNSSKRLGL
                     DHVDLFLIHWPNPSLGKYVEAWRGMIELLEQGDVRAIGVSNFKPSHLQRLIDETGVVP
                     HVNQIQLNPWMSRAGEREYHSSHGIVTESWAPIGKGGGLLGDPVIADMARAKSRTPAQ
                     VVLRWHLQLGLIPIPKTSRRERLAPNLDLFDFSLSDEEMVIIDELDNGERGAVDSDTT
                     GL"
     gene            3848..6145
                     /gene="lon"
                     /locus_tag="VFU96_11125"
     CDS             3848..6145
                     /gene="lon"
                     /locus_tag="VFU96_11125"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEU5113859.1"
                     /translation="MRETAQLTLPVLPLHNGVVFPHMVVTIRIETEEGRTAIAAASKA
                     DGRLLLVPRLDGRYAAVGTIADIQEADTEGNIVVSGVSRARVGAGSVDETGALWVDAE
                     SSPEVDIIDDELDALVAEYRAVVIAVLENRGMAGVAERLLDVDNPSRLADLAVYSPDL
                     SLEQKVELLEALDIRQRLGKLLVWMREVLADLELRRKVRDEATEQIEKGQKDYILRQQ
                     MEAIRKELGEDANVASEYRAKLSERDLPEKVADAIEREIGRLERMSEQSPEHSWIRTW
                     LDTMFDLPWGVSSEDRLDLDEAWTVLDADHTGLTDVKERIIEHLAVRKLRAERGLTDS
                     DDRNTGTILLLVGPPGVGKTSLGESVARALGRKFVRVALGGVRDEAEIRGHRRTYVGA
                     RPGRIARALTEAETMNPVFLLDEMDKVGADWRGDPSSALLEVLDPAQNHTFRDHYLEV
                     DLDLSQVLFIGTANVLETIPGPLLDRTEVINIDGYTDAEKVEIARDHLLPRQLRAAGL
                     EPEELEVTEGALAAVVDRYTREAGVRNLERELGKLARKVATEVAKESGSDSVVVDEHS
                     VRDYLGKPKVWKDEASDRTDVPGVATGLAVTGAGGDVLFIEATAMDGEPGLTLTGQLG
                     DVMKESARIALSYVEAHSGELGIDPSALTKRYHVHVPAGAIPKDGPSAGVAMLTALVS
                     LLTGRKVKDTVGMTGEITLQGKVLPIGGVKQKVLAAHRAGLKEVIVPRRNEADLDDVP
                     EKVRDVMAFTIADTASDVLEAALQA"
     gene            complement(6501..7163)
                     /locus_tag="VFU96_11130"
     CDS             complement(6501..7163)
                     /locus_tag="VFU96_11130"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU5113860.1"
                     /translation="MTANVLADRADVAHLSRVLNEVDPDVLLTQELGHVAAETIAAHF
                     PHHDLRPNEDARGRGIASRLDAEFGEIPLPWRPGLWARVLGDESPSFLVANIHMRNPV
                     VFPWWQSARIRGRQLDSLFAWADDSVQPDMPFILGGDMNASPAWPVYKVLAERWDDLV
                     ARSAEESGVSTEPTWAWRPGWPRLLRIDHVFGTGARAEAARVEPLPGSDHAAVIVDLL
                     FD"
     gene            7316..7567
                     /locus_tag="VFU96_11135"
     CDS             7316..7567
                     /locus_tag="VFU96_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113861.1"
                     /translation="MRGSGSVVASIVGNALIGELTANCEHDGESDRGEYVVGHSGRAI
                     TWRVRFCDPCWDHFRAHQEVIDLSLFEDAVDDLQVESSR"
     gene            complement(7580..>7902)
                     /locus_tag="VFU96_11140"
     CDS             complement(7580..>7902)
                     /locus_tag="VFU96_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113862.1"
                     /translation="KTLPNGDFDRSFNQGLLMLDVLRNLKEKGFMAIPKLLELSGPFM
                     MTDLGPAELLTFAAQTIATKKEAIPNVVAPGSAGMAGQASVVFLSDSAPRVFRDLADG
                     RLGN"
BASE COUNT         1498 a         2534 c         2530 g         1340 t
ORIGIN      
        1 ggggtcgtag accctgacca tcggcatttc gatctgatcc gcctcaccac cgtcgcggcc
       61 gacgaacacc agctccaggt cgtccgttcc ccgcccttct gccactgcgt gcggcccgac
      121 aatggcaagc gcccggatcg agtcgttgtc ccgtgacctc tcgtcggcct cgagcaccgc
      181 gacgtgggct ccgggatgtt cggtcatcag caaagcgagc aagagctcgt cacgcgcctc
      241 tgggactgtt tcggactcca gggcgtcccg caggacactt cgcccctcga agcctcgtgc
      301 catcgcccag taggcatgac cccggatcat cgaggaggcg tgacgggtgt gagcctcgac
      361 cacctcccgc gcatcgggct cccccgagtt ccccgcggca acgaggaggt ttcgccggat
      421 gaagcgtccc tgtctcctgg gcacatacca ccagctgtat cggtcgagga ggtcaccgtc
      481 ggaccgcccg agcaagtcgc caaaccggag gttccccggc gtctcccctg ccgcagccag
      541 ggcgcgtccg cccggtgggc acgccgtcaa acactcgtcg cacccgtaga ttcgccggcc
      601 gatgagcggc cggatccact gtgggatcga tccgggagtc tgcagccagg tggacaaaca
      661 gcggcgtgcg tcgagacccc actcccccaa tgccccggtt ggacatgccg ggatgcatgt
      721 cgagcaggac ccgcagtctc gctccatggg tctgctgaca gggatcgagg cgtccgtgac
      781 cacggttccg agcagcatcc aaggcccatg cccgggcgcg agcaccatcg tgctctttcc
      841 cggccagccc acgcccgccc gaactgcggc cgccctatcg acgaggcggt tgtcgtcaca
      901 cagtgtctcc gcccgatatc cacccgcctg gaggaacccg gcgatcgcgt cggtgatgcg
      961 acggactccg gcgtaatgat tggtggtcgc gaaccgtccg acgagtgccc cttcgtctgt
     1021 aggggtctcg gcctgttgca ggtaggcgta gctcaccacg accagcgacc tggcccaggg
     1081 gaatgacaaa gagatgtcag tggccacgtc ggggtcgtcg tatgtgaagc gaagcggccc
     1141 ggagcgaccc gattcgcggg acgcgcgcag tgctgccctc tcccgggaaa aggtctcggc
     1201 accggtcacg ccgaaaccgg cggcgcccat gcggtgggcc agctccgaaa gctcctcggc
     1261 caagatcatg tccgaatatt gccctcgtag cctcgttccc acacgctttg ggcgtaagtg
     1321 gttcagaaat gggcaggtga gagcggtgcg aatcctgtac gcgttcgtgg tgatgaccgt
     1381 tgatggcttc tacgaaggcc cagacggtga gttcgactgg ccgaatgtcg acgacgagtt
     1441 cgaccggtac tcggtcgcca gcctcgaaga caccggcttg atcgtctttg gtcgggtcac
     1501 atacgaaggg atggcgagct attggccgac ggccgcggcc ctggaggagg acccgatcat
     1561 cgccccactc atgaacgaga tcccgaaagc agtcgtgtcg agcactatcg agaatgcggc
     1621 ctggaacaac tccacggtga tcagaggtga tgtcgccaac gagatctcga agctgaaggc
     1681 tcagtcgggc aagtcgatcg gcgtcttcgg cagcccgaat ctgacactga gcctgatcga
     1741 aatgggtctg gtcgatgagc tgagactcat gatccacccc gtggcgctcg gttcaggcaa
     1801 ttccctcttc aggacgatga cgggcagact caggttcgac ctgctgggga cccacacctt
     1861 cgaatcgggg aacgtgctcc tcacctaccg gccgggagta ccgaccgggt tggcatgacg
     1921 tgaggagatc gatcaccgac acgccggtga cccggattca ggcggatcac agcggagtct
     1981 gttggtgaag gaccagtcgc caggcaccgc cccggttcac atacacgctg ctgattgccg
     2041 ccctgtatcc gtcatcacgg ccgtcacggg ttgcatccgc ggtgtacacc agagcggtca
     2101 cgtcgtcacc gacgtcgacg aaccgaacat cgtcgatgct gaacctggac cagggcgtgg
     2161 cctcctccat ggccgagatc acctgggcct ttgtcatcag cccgtcagga agggccatga
     2221 cgccgtcgtc ggcgaaattc tcccgataga acctgggatc gcctgccgat tcccagaagg
     2281 ccgattccaa ctccagtatt cgagtctcgc tcgtcgtgct cgatttcgac atccgaacca
     2341 cctacccggt tgacgacaaa ggcaaccgcc ctcactcgac gacgatgctc ccacgcatcg
     2401 ttgggtgcac ctcacagaag taggcaaagg tccctggctc gtcgaaggtg aacgagaact
     2461 cgcctccctc attgagcgtc cccgaatccc agagctcgtc gtcggaggtc gaggtgtggg
     2521 gcaggtccgg gtcggcgttc acccaggtca ctgtgctgcc ggcggccacc gtgagggtct
     2581 ccgggttgaa ggcgagatcg gcgatctcca ccctctgctc ctcaccgcct gccccgtcat
     2641 tggcccctgt cgtgtcgccg ctctccccgc tgcacgcggc gagcatcgat gtgagggcga
     2701 tcgccaggaa gaagacacgt ctcactggaa gctccttgtt cgcttgcggg tgcattacga
     2761 gcccgaggcc ggttgggttc acgcgcgatc ccgtggacgg caatggtcgt gcgggtgtcc
     2821 cggcccccta tcgtgcctcc gtgcttcgta cccccgagac cgtccatgcc ccctgatctc
     2881 cacgatcgag tcaccctgtc caacggcgtc gccatgccga tcatcgggta cgggacgtgg
     2941 ccgataccgg atcgagaagc ggagaatctg gtcgcggtgg ctctcgatgt cggataccgg
     3001 ttgatcgaca ccgccgagat gtatcgaaac gaagaagggg tgggacgggg gatacgagca
     3061 agtgggatcg atcgcgatga tgtgttcgtc acgacgaagt tgagccagag atggcacggg
     3121 tacgaggagg cgcagcaagc attcaccaac agctccaagc gcctcgggct cgaccacgtc
     3181 gacctcttcc tgattcactg gcccaacccg agtctgggaa agtacgtcga ggcatggcgg
     3241 gggatgatcg agctgttgga gcagggtgac gtcagagcga tcggcgtctc caacttcaag
     3301 ccatcccacc ttcagcgact gatcgacgag accggggtcg ttccgcacgt gaatcagatc
     3361 cagctgaacc cctggatgtc gcgggcgggc gagagggagt accacagcag ccatgggatc
     3421 gtgaccgaat catgggcgcc gatcgggaaa ggcggcggcc tgctcggcga cccggtgatc
     3481 gccgacatgg cccgggcgaa gagccggacg ccggcccagg tggtgctgcg ctggcacctc
     3541 cagctcgggc tgatcccgat accgaagacc tcccggcgag agcgcttggc gcccaacctc
     3601 gaccttttcg acttctccct aagcgacgag gagatggtga tcatcgacga gctggacaac
     3661 ggcgagcgtg gggcggtcga ctccgacacc accggtctct gaatcagcag gaagatcggc
     3721 gggcgatcat ggcctgcccc cttcgggctg accaaacctg agcacgatct actcagatcg
     3781 tggaatagga gtttcgggct gcttgttgca tcggtttcaa cttgcaatac gaaggaatac
     3841 cgagctcatg cgcgagaccg cccaactgac cctccctgtt ctccccctcc acaatggtgt
     3901 cgttttcccc catatggtgg tgacgatccg gatcgagacc gaagagggtc gaaccgcgat
     3961 cgccgctgca tcgaaggctg atggacgtct cctcctggtc ccgagactcg acgggcgtta
     4021 cgccgcggtc ggcacgattg cagacatcca ggaggccgac accgagggca acatcgtcgt
     4081 gtcgggtgtc tcccgggcgc gggtgggagc gggctcggtc gacgagaccg gagccttgtg
     4141 ggtcgacgcc gagtcttcac ccgaggtcga catcatcgac gacgaactgg atgcgctcgt
     4201 cgccgagtac cgggcggtgg tcatcgccgt cctcgagaac agaggcatgg ccggagttgc
     4261 cgagcgtctc ctcgatgtgg acaacccgtc gcgtctggcc gacctcgccg tctactcgcc
     4321 cgacttgtcc ctggagcaga aagtcgagct cctcgaggcc ctcgacatcc ggcaacgact
     4381 cggcaagttg ctcgtctgga tgcgtgaggt cctggccgac ctcgagctcc gtcgcaaggt
     4441 gcgtgacgag gccacggagc agatcgaaaa ggggcagaag gattacatcc tccgtcagca
     4501 gatggaggcg atccgcaagg aactgggcga agacgccaac gtcgcgtccg agtaccgggc
     4561 caagctctcc gagcgggatc ttcccgagaa ggtggccgat gccatcgaga gggaaatcgg
     4621 acgtctcgag cggatgagcg agcagtctcc tgagcactcc tggatccgca cgtggctgga
     4681 cacgatgttc gatctgcctt ggggcgtctc atcagaagac cgactcgacc tcgatgaggc
     4741 ctggacggtg ctcgacgccg accacaccgg tctcaccgac gtcaaagagc ggatcatcga
     4801 gcacctggcg gtgcgcaagc tacgtgccga gcgtggcctc accgacagcg acgatcgcaa
     4861 cacagggacc atccttctcc tcgtcggccc ccccggcgtc ggcaagacct ccctgggtga
     4921 gtcggtggcc cgtgcccttg gtcgcaaatt cgtccgtgtc gccctcggcg gggttcggga
     4981 cgaagccgag atccgcgggc atcgccggac ctatgtcggg gcgcggccgg gacggatcgc
     5041 tcgtgccctc accgaggcgg agaccatgaa cccggtgttc cttctcgacg agatggacaa
     5101 ggtcggtgcc gactggcgcg gcgacccgtc ctccgctctc ctcgaggtgc tcgaccctgc
     5161 ccagaaccac acattccgag accactacct agaggtcgac ctcgacctct cccaggtcct
     5221 gttcatcggc accgccaacg tgctcgagac cattcccggg cccctgttgg accggaccga
     5281 ggtgatcaac atcgatggct acaccgacgc cgagaaggtg gaaatcgcac gtgaccacct
     5341 acttccccgc cagctccgcg ccgcggggct agagcccgag gagctcgagg tcaccgaggg
     5401 tgccctcgcg gcggtggtcg accgatatac ccgggaagcc ggggtgagga acctggagcg
     5461 agagctgggc aagctcgcac gcaaggtggc caccgaggtg gccaaggagt cgggatcgga
     5521 ttcggtcgtt gtcgacgaac attcggtcag ggactacctg ggcaagccca aggtatggaa
     5581 agacgaggcg tcggatcgca ccgatgttcc tggagtggct accggtctgg ccgtcaccgg
     5641 cgcgggcggg gatgtgttgt tcatcgaggc gacggccatg gatggcgagc ccgggctcac
     5701 attgaccggg cagctaggcg atgtgatgaa ggagtcggcg aggatcgccc tctcctatgt
     5761 cgaggcgcat tccggcgagt tgggaatcga cccgtctgcg ctgaccaaga ggtaccacgt
     5821 ccacgtcccc gccggcgcca tccccaagga cggcccatct gctggtgtgg cgatgctcac
     5881 cgcgttggtg tccctgctga ccggtcgcaa ggtcaaggac accgtcggaa tgaccggcga
     5941 gatcacactt caggggaagg tgctgccgat tggcggggtc aagcagaagg tgctcgccgc
     6001 acaccgggcc ggcctgaagg aggtcatcgt gccacgtcgt aacgaggccg acctcgacga
     6061 cgtaccagag aaggtccggg acgtgatggc cttcaccatc gccgacaccg cgagtgacgt
     6121 gttggaggcg gccctccagg cctgacctca ctcgcctgcc cggtgcctgc cgagagaagc
     6181 atctgactcc acatccgagg gtcgagcgaa gaccgatctg tcgacaacgg accctgctgg
     6241 tatgccgcca gctggaacac gaacttcgaa gtgcccgcac gattcacaca tcacacgttc
     6301 gaagcccagt gagtgaaccg aaactgtggt gcgatgacga caacgactct gccgcaattt
     6361 gcgactcagg acgcgcatct aaacaagcct cccctggagc tgccaacccg gcaggaaggc
     6421 tgccctgagc ggcgccgacg agccataggt cggacggctc agtcaccagc cattcgacac
     6481 ctcagatagg gagcccgggc tcagtcgaac aagagatcga cgataacggc ggcatgatcc
     6541 gatcccggca gcggctccac tcgggccgct tccgctctgg cgccggtccc gaaaacatgg
     6601 tcgatgcgga ggagacgagg ccaccctgga cgccaagccc aggttggctc cgtcgaaacg
     6661 ccggactcct ccgcggatcg agcaacgaga tcgtcccacc gctctgccaa caccttgtag
     6721 acgggccatg ccggtgaggc attcatatcc ccacccagga tgaagggcat gtcgggttgg
     6781 accgagtcgt cggcccaggc gaacaacgaa tccagttgcc ggccccggat gcgggccgat
     6841 tgccaccatg ggaacacgac agggttcctc atgtggatgt tggcgacgag gaacgacggc
     6901 gattcgtcgc ccaacacccg agcccagagc ccgggccgcc agggcagagg tatctccccg
     6961 aactccgcgt cgagtcggct ggcgatgccg cgcccgcggg catcctcgtt tggccgcagg
     7021 tcatgatgag ggaagtgcgc ggcgatcgtc tccgccgcca catgaccgag ttcctgcgtg
     7081 agcagcacgt cggggtcgac ctcgttcaac acccgggaca gatgagcgac gtcggcgcgg
     7141 tccgccaaca cattggcggt catcagtcgg atgggtctct gcatggcgcc gcacgctaac
     7201 ccggcaccgg cacaactcag ggcggcgaaa aactcctcag aagttgcttg ggtcgctgga
     7261 cctatatgcc gagtgaatag atgtgatagc tgctgctgat accaaggtca cattggtgcg
     7321 aggctcggga agcgtcgtcg cctcgatcgt gggcaacgcc ctcatcggcg agctgaccgc
     7381 caactgtgag cacgacggag agagcgaccg cggcgagtac gtggtcgggc actcgggccg
     7441 ggccataacc tggcgtgtcc ggttctgtga cccttgctgg gaccatttcc gggcccacca
     7501 ggaagtaatc gacctcagcc tcttcgagga cgcagtcgac gaccttcagg tagagagcag
     7561 tcgctgaccc gctccacggt cagttaccga gacggccgtc ggcgaggtcg cggaagacgc
     7621 ggggcgcgga gtcggagaga aacacgacgc tggcctggcc ggccatgccg gcggatccag
     7681 gtgccacgac gttgggaatg gcttccttct tggtagcgat ggtctgcgcc gcgaacgtaa
     7741 gcagctctgc cgggccgagg tcagtcatca tgaatggccc ggagagctcg aggagcttcg
     7801 ggatcgccat gaagcctttc tctttgagat tcctcagcac atcgagcatg agaaggccct
     7861 gattgaacga tcggtcgaaa tcgccattgg gaagggtctt ac
//
LOCUS       DASTXO010000524         4228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_142715, whole genome shotgun sequence.
ACCESSION   DASTXO010000524 DASTXO010000000
VERSION     DASTXO010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142715"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VFU96_11145"
     CDS             <1..1081
                     /locus_tag="VFU96_11145"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEU5113863.1"
                     /translation="AQQECLAAVDRTDAESCWVVVLDVESGGILALAGAPVFDPQTRQ
                     TVDETCDPEHEPTCHAFKNFIVRGVYEPGSTQKLITVAAAIEEGEVKAGTVIPEVADL
                     LELRPDACRSADDDLFGCYRDFEEHETASMSVREIFTRSSNVGTILVADKLGQDRLVS
                     YIERFGLGSKTGVDYSVEAPGTLNFEAGCETCWASAAIGYSVAATPLQMAAAYAAIGN
                     DGIWTAPHIVASTTDVDGNQDVAGLETRRVVSDDTAVLMQELLANVVDEGTGQAATVE
                     GYQVGGKTGTANKLGEDGRYTEVTRASFVGMAPMSDPKVVVAVLIDAPSFRYRTGGAA
                     AAPVFANVMEQALHRLGVPPDVGAG"
     gene            1086..2552
                     /locus_tag="VFU96_11150"
     CDS             1086..2552
                     /locus_tag="VFU96_11150"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU5113864.1"
                     /translation="MGDVSGRAGGLVVGDPDIWLGDVTHDSALAGPDVLFVAVRGATH
                     DGHDYVRSALAAGSPAAAVEKLLDVPVTQIVVGDTRAAMGPMAARVHGDPSSKMAVVG
                     VTGTNGKTTVTHYLESLVTSAGRVVGLIGTVETRVGETALPSVRTTPEATDFQRLLAE
                     MSRLHADVVAAEISSHALEMSRVAGTRFEVAAFTNLSQDHLDFHGTMESYRRAKERLF
                     RDYEVGAAVVNIDDPVGAAIASWVDVPLIRVGHAGEVHAENIETTIQGTRFDLVTDRG
                     AIAVASDLIGAFNVENALVAAGCALALGLSVEEISHGLASVGSVPGRFEQITPSGDIQ
                     VIVDYAHTPDGVEEAIAVARSIGSGRVIAVVGAGGDRDREKRPLMGRAATGADLAILT
                     SDNPRSEDPDQILAEVAEGASGPWVVLEVDRRRAIESGIAAAEAGDVVLILGKGHETG
                     QEIRGQVVPFDDRQVAREILATRSGSADFDPDSGSMTT"
     gene            2549..3601
                     /gene="mraY"
                     /locus_tag="VFU96_11155"
     CDS             2549..3601
                     /gene="mraY"
                     /locus_tag="VFU96_11155"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU5113865.1"
                     /translation="MIPLLTAAAVGLLVSIFGTPYAIRFFRRREIGQFIQEEIAGMHG
                     HKQGTPTMGGVVFVLAAIIGYLVSHVRVVAPAGGMGLEFTPLSDGGLLTVFALAGMAL
                     VGFLDDWIKYSNKRSLGLSKSAKFGGQLAIASLFAWGATQAGVVTEISFVRPLGIDLG
                     AFFVIWVLLMLTGWANGVNLADGMDGLAGGSSALVVASYTVIAFWQFRNPGVYESVDP
                     LELAVISAAMFGAILGFLWWNAPPAKIIMGDVGSQGIGGLLAALALLTNTSLLLVVLG
                     AIFVAETASVILQVASFKIFGKRIFRMSPIHYHFDLGGWPETTVVIRFWILTGLGVAL
                     GLGFFYGDFLRLEGLL"
     gene            3598..>4228
                     /locus_tag="VFU96_11160"
     CDS             3598..>4228
                     /locus_tag="VFU96_11160"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU5113866.1"
                     /translation="MTRVLVLGAGVSGLAAATLARDRDMKVTIYTRDQPELALEKGFA
                     VASGGWDPVLLTGIDLVVASPGFPERALPIVEALEWGIPVWSEIEFAWRHLDVPVAAV
                     TGTNGKTTVTEATSAMLVASGIDAPAAGNVGSALSEHVGRGHEAVVVEVSSFQLRFCE
                     EFHPTAAAITNLAIDHLDWHGSEHSYRAAKARIFLNQGPDDLLVFDADDE"
BASE COUNT          689 a         1329 c         1436 g          774 t
ORIGIN      
        1 ggctcaacag gaatgtctcg ctgcggtcga ccgcaccgac gcggagtcgt gttgggtggt
       61 ggtgctcgac gtcgaatcgg gaggaatcct ggctctggcc ggcgcccccg tgttcgatcc
      121 gcagacgcgc cagacggtcg atgagacctg tgatcccgag catgagccga cctgtcacgc
      181 cttcaagaat ttcatcgttc gtggtgtcta cgagccgggg tcgacccaga agctgatcac
      241 cgtggccgcc gccatcgagg agggcgaggt gaaggcggga acggtgatac cggaggtcgc
      301 cgacctcctg gaactgcgcc cggacgcctg tcgttcggcg gatgacgact tgttcggctg
      361 ctaccgcgac ttcgaggagc acgagacggc aagcatgagt gtgcgggaga tcttcaccag
      421 gtcctcgaat gtgggaacga tccttgtcgc cgacaagctg ggacaggaca gactcgtttc
      481 ctatatcgag cgattcggcc tcggctcgaa gaccggggtg gactactcgg tcgaggcgcc
      541 cggcacgctc aacttcgagg cgggctgtga gacatgctgg gcctcggcag cgatcggtta
      601 ctcggttgcc gcgactccgc tccaaatggc cgcggcctac gccgccatcg gcaacgacgg
      661 gatctggacg gctccccata tcgtcgcgtc gacgaccgat gtcgacggca atcaggacgt
      721 cgccggcctg gagacgagaa gagtcgtctc cgacgacacg gctgtcctga tgcaggaact
      781 gctggccaat gtggtcgacg agggcaccgg gcaggcggcg acggtggagg gctaccaggt
      841 cggcggcaag accggtacgg cgaacaagct gggcgaggac gggcgttaca ccgaggtgac
      901 cagggcctcg ttcgtcggta tggcgccgat gagtgacccg aaggtcgtgg tggcggtcct
      961 catcgacgcc ccctctttcc gctaccgaac cggaggggct gcggctgcgc ctgtgttcgc
     1021 caacgtcatg gagcaagcgc tgcaccgatt gggagtgccc cccgatgtcg gcgccgggta
     1081 gcaccgtggg cgacgtatcg ggccgggctg gaggcctcgt ggtcggcgac cccgacatct
     1141 ggctcggcga cgtgactcat gattcggcac tagccggtcc cgacgtcctg ttcgtcgcgg
     1201 tcaggggggc gacccatgac ggccacgact atgtgcgatc ggcgcttgcc gccggctctc
     1261 cggctgccgc cgtcgagaag ctgctggacg tgcccgtgac tcagatcgtt gtcggcgata
     1321 cgcgggccgc gatgggcccg atggcggccc gggtccatgg cgatccttcc tccaagatgg
     1381 cggtggtcgg ggtcaccggc accaatggga agaccaccgt cacccattac ctcgagtctc
     1441 tcgtgacgtc cgcaggccgt gtggtagggc tgatcgggac cgtcgagacc agggtggggg
     1501 agacagccct gccatcggtg cggaccacgc cggaggccac cgactttcaa cgcctcctcg
     1561 ccgagatgag ccgactccac gccgatgtgg tcgcagccga gatctcgtcg catgccctcg
     1621 agatgagccg tgtcgccgga actcgcttcg aggtcgctgc gttcaccaac ctgtcccagg
     1681 accatctcga tttccacggg accatggagt catatcggcg ggccaaggag cgccttttcc
     1741 gggactacga agtgggagcg gcggtggtca acatcgacga cccggtcggc gccgcaattg
     1801 cctcctgggt ggatgtcccc ctcatccgag tgggacacgc cggcgaagtc cacgccgaga
     1861 acatcgagac aacgattcag gggacgcggt tcgacctggt gaccgaccgt ggggcaatcg
     1921 cggtggcgtc agatctgatc ggtgctttca atgtggagaa tgccttggtg gcggcgggct
     1981 gtgctctggc cctcgggctg agcgtcgaag agatctcgca tgggctggca agtgttggca
     2041 gcgtgcccgg tcgattcgaa cagatcactc ccagcggtga catccaggtc attgtcgact
     2101 atgcccacac ccccgacggc gtcgaagagg cgatagccgt agccaggtcg atcgggtcgg
     2161 gcagggtgat cgcggtggtg ggagccggcg gcgaccgcga ccgcgagaag cggcctctga
     2221 tgggccgcgc tgccaccgga gccgatctgg cgatcctcac cagcgacaac cctcgttcgg
     2281 aggacccgga ccagatcctc gctgaagtag ccgaaggcgc ctccggcccc tgggtcgtcc
     2341 tcgaggtcga ccggcggcgc gctatcgagt ctgggatcgc ggccgccgaa gcgggggacg
     2401 tggttctgat cctgggcaag ggccacgaaa ccggtcagga gatccggggc caggtggtgc
     2461 cgttcgacga ccgacaagtg gctcgggaga tcctggccac caggtccggg tccgccgatt
     2521 tcgaccctga ttcagggagc atgaccacgt gataccgctg ctgaccgccg ccgcagtcgg
     2581 gctcctcgtg tcgatcttcg gcactccgta cgccattcgt ttcttcaggc ggcgtgagat
     2641 aggccagttc atccaggagg agatcgccgg gatgcacggt cacaagcagg gaacgccgac
     2701 catgggtggc gtcgtgttcg tgctcgccgc catcatcggg tacctggtgt cacacgtccg
     2761 cgtcgtggca ccggccggcg ggatgggcct cgagttcacg ccgttgagcg acggcgggct
     2821 gctcacagtg ttcgccctgg ccggcatggc attggtcgga ttcctggacg actggatcaa
     2881 atactccaac aagcggagtc tcgggctctc caaatcggcc aagttcggcg gacagctggc
     2941 gatcgcctcc ctgtttgctt ggggcgccac tcaagccggt gtcgtcaccg agatctcatt
     3001 cgtcaggccg ttgggcatcg acctgggcgc gttcttcgtg atctgggtgc tgctgatgct
     3061 gaccggctgg gcgaatggag tgaacctcgc tgatggcatg gacggcctgg ccggagggtc
     3121 atccgccctg gtcgtcgcct cgtacacggt cattgccttc tggcagttcc gaaatcccgg
     3181 tgtctacgaa tcggtcgacc ccctcgagct ggccgtcatc tcggcggcga tgttcggcgc
     3241 catcctcggc tttctctggt ggaacgcccc tccggccaag atcatcatgg gcgatgtcgg
     3301 ctcccaaggg atcggggggc ttctggccgc cctcgccctc ctcaccaaca ccagtctcct
     3361 cctggtcgtc ctgggagcga tcttcgttgc cgagacggcg tcagtgatcc tccaggtggc
     3421 gagtttcaag atcttcggga agaggatctt caggatgtcg cccatccact atcacttcga
     3481 tttgggcgga tggccggaga cgacggtcgt gatcaggttc tggatcctga ccggcctcgg
     3541 ggtcgccttg ggcctcgggt tcttctacgg agacttcctc cggctcgagg gcctgttgtg
     3601 acccgggtgc tcgtcctcgg cgctggtgtg tccggtctgg ctgcggccac gctcgcccgc
     3661 gaccgggaca tgaaggtgac gatctacacc cgggaccagc ccgagctcgc cctggagaag
     3721 ggcttcgccg tggccagcgg cggatgggat ccggtcctcc tcacaggcat cgatctcgta
     3781 gttgccagcc ccggcttccc cgagcgtgca cttccgatcg tcgaggccct ggaatgggga
     3841 atcccggtat ggagtgagat cgagttcgcg tggcgtcacc tggatgtgcc ggtcgcggca
     3901 gtgaccggta ccaacgggaa gacgactgtc accgaggcca catcggccat gctcgttgcg
     3961 tcggggatcg atgcccccgc ggcaggcaac gtcggctcag ccctgtccga gcacgtcggc
     4021 cggggccacg aggcagtggt agtcgaggtt tccagctttc aactcagatt ctgcgaggaa
     4081 ttccacccta ccgcggccgc catcaccaat ctcgctatcg accatctcga ctggcacggg
     4141 tccgagcact cataccgggc cgccaaggcg aggattttct tgaaccaggg ccccgatgac
     4201 ctgctggtgt tcgatgccga tgacgaag
//
LOCUS       DASTXO010000525         2205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_252715, whole genome shotgun sequence.
ACCESSION   DASTXO010000525 DASTXO010000000
VERSION     DASTXO010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252715"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..684
                     /gene="paaC"
                     /locus_tag="VFU96_11165"
     CDS             1..684
                     /gene="paaC"
                     /locus_tag="VFU96_11165"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEU5113867.1"
                     /translation="MAHADDNLILAQRLGEWISRAPELEEDIALGNIALDHLGVARAL
                     YTRAGALEEKGRGEDDLAMRRSEREFLNLLLVEQPNGDFAHTMVRGVLFDLYQVELWA
                     DLSFAEDTTLAGIAARALKEARYHVLHSSAWVIRLGDGTEESHRRAQTAVDNLWRYTA
                     ELFEGPAQGYRDPWEKGVGEILEEAHLRIPDDPYQRSGGRRGFHSEHLGPLLAEMQWL
                     QRSHPGLEW"
     gene            678..1142
                     /gene="paaD"
                     /locus_tag="VFU96_11170"
     CDS             678..1142
                     /gene="paaD"
                     /locus_tag="VFU96_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014444461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HEU5113868.1"
                     /translation="MVSVAVTLDQDRARAVAARVVDPEIPVLSIAELGILRDVRLEGH
                     RVIATITPTYSGCPAMRQIEDDLRATLRAAGFDDVEVVTEHEPPWSSDWITPDGLTKL
                     AGFGIAPPGTEILCPRCQSGSPRVVSRFGSTACKALMACRSCGEPFDHFKDF"
     gene            1144..>2205
                     /gene="paaE"
                     /locus_tag="VFU96_11175"
     CDS             1144..>2205
                     /gene="paaE"
                     /locus_tag="VFU96_11175"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HEU5113869.1"
                     /translation="MSVDFHPLRVIEVAPLTEDSIAVTFDVPEHLDAVFRYVPGQHVT
                     LRAEIDGEDLRRSYSICSSAVGGKLRVGIKRLPGGVFSAWAIDELRAGDVLGVMPPVG
                     EFTIDPDPNQPGHRVAIAAGSGITPVLSLIATTLETEPGSSWTLLYGNRTANSVMFLE
                     ELEGLKDRYTGRFQLFHLLSREGSDLPLLSGRIDDAKIRVVHDRLLGGREVDGWYLCG
                     PYDMVMEARRTLADLGVDEARIHDELFFAGRLDLGSLPPEPPPGEGSVDLTVILDGRA
                     VETRMTPETSILDAALRVRSELPFSCKGGMCASCKGRIEEGEVSMDKNYALVDSELEA
                     GYVLTCQSHPTSNRVVVRYD"
BASE COUNT          364 a          709 c          727 g          405 t
ORIGIN      
        1 atcgcccatg ccgacgacaa cctgatcctg gcccaacggc tcggtgagtg gatctcccgg
       61 gcccccgagc tggaagagga catcgccctg ggcaacattg ccctcgatca cctcggcgtg
      121 gcccgcgctc tgtacacccg cgcaggggcg ctagaagaga aagggcgcgg cgaggacgat
      181 ctcgcgatgc gacggtcgga gcgggagttc ctcaacctcc tcctggtgga gcagcccaac
      241 ggcgatttcg cccacaccat ggtgcgaggt gtcttgttcg acctgtatca ggtcgagctc
      301 tgggccgacc tgtcattcgc cgaagacacg accctggccg gtatcgcggc gagggccctc
      361 aaggaggccc gctatcacgt ccttcactcc tcagcctggg tgatccgtct cggtgacggc
      421 accgaggagt cccatcgtcg agcgcaaacg gcggtcgaca acctctggag atacaccgcc
      481 gagctgttcg aaggtcccgc acaggggtat cgcgacccct gggagaaagg agtcggtgag
      541 atcctggagg aagcccacct acgaattccc gacgatccct atcagcgcag tggaggccgc
      601 cgcggctttc actccgagca tctcgggccg ctcctcgccg agatgcagtg gttgcagcgc
      661 agtcatccgg ggctggaatg gtgagcgtgg ccgtcacgct cgaccaggac cgggcccgcg
      721 ccgtggcggc gcgtgtcgtt gaccccgaga ttcccgtgct cagcatcgcc gagctgggca
      781 tcctgcgcga tgttcgcctc gaggggcacc gggtgattgc gaccatcacg ccgacatatt
      841 cgggatgccc ggccatgcgc cagatcgagg atgacctgcg cgccacgctc cgagcggccg
      901 gcttcgatga cgtggaagtg gtcactgagc acgagccgcc gtggagcagc gattggatca
      961 caccggatgg cctgaccaag ctggccggat tcgggatcgc accacccgga acagagattc
     1021 tctgcccgag gtgccaatcg gggtcgcccc gggttgtctc caggttcggg tccacggcct
     1081 gcaaggcgct gatggcctgc cggtcgtgtg gcgagccatt cgatcacttc aaggatttct
     1141 gagatgagcg tcgacttcca cccgttgcga gtgatcgagg tcgctccact caccgaggac
     1201 tcgatcgccg tgacctttga cgttcccgag cacctcgacg ctgtgttccg ctacgtcccg
     1261 gggcagcacg tcacgctgcg ggcggagatc gatggcgagg atctgcggcg ttcctactcg
     1321 atctgttcca gtgcggtcgg cggcaagctc cgggtgggga tcaagcgttt gccgggaggc
     1381 gtgttctcgg cttgggccat cgacgagctg agagccggcg acgtcctcgg ggtcatgccc
     1441 ccggttgggg agttcactat cgatcccgat ccgaatcagc cgggtcatcg ggtcgctatc
     1501 gctgccggct cgggaatcac gccggtgctg tccctcatcg ccaccaccct cgagaccgag
     1561 ccaggctcgt catggaccct cctctatggg aacagaactg ccaattcggt gatgttcctg
     1621 gaggaattgg agggtctgaa ggatcgctac accggccgtt ttcagctgtt tcacctgctc
     1681 agccgggagg gctccgattt gccacttctc tcgggccgca tcgacgacgc caagatccgg
     1741 gtcgtccacg accgcctcct cggtgggcgg gaggtagacg gttggtacct ctgtggtcct
     1801 tacgacatgg tgatggaggc gcggcgcacc ttggccgacc tgggggtcga cgaagcccgt
     1861 atccacgatg agctcttctt tgccgggcgc ctcgacctcg gttccctgcc gccggagcct
     1921 ccgcccgggg aggggtcggt cgatctcacc gtcattctcg atgggagggc cgtcgagacg
     1981 aggatgacgc cagagacgtc gatcctcgac gccgctctga gggtgcgatc cgagcttccg
     2041 ttctcgtgca agggcggcat gtgtgcctcg tgcaaggggc gcatcgagga gggcgaggtg
     2101 agcatggaca agaactacgc cctggtcgac tccgagctcg aggccggata cgtgctcacc
     2161 tgccagtcgc accccacttc gaatcgggtc gtggtccgtt acgac
//
LOCUS       DASTXO010000526         2418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1321511, whole genome shotgun sequence.
ACCESSION   DASTXO010000526 DASTXO010000000
VERSION     DASTXO010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321511"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VFU96_11180"
     CDS             <1..674
                     /locus_tag="VFU96_11180"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5113870.1"
                     /translation="HFREGTVKPYQQHDEDFMREVAEGLKTLPRIFLDLVLPQLPDVE
                     QRLSQGGRVLDVGCGGGWAVVQIAERFPDVTCLGVDVEPYSVALAQQLILDRGLADRC
                     EARAMSVDELDEDGAFDVATSFLVVHEIAPSLKDAAFASIARALKPGGYFLIFDEVYP
                     VNDEDLRRMPTRFAALAQWYELTWGNVVDTRAGLEELCSQAGLAVIDETGFSRFHIVV
                     AEKPV"
     gene            complement(698..1012)
                     /locus_tag="VFU96_11185"
     CDS             complement(698..1012)
                     /locus_tag="VFU96_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113871.1"
                     /translation="MAKFLFVYHGGSAPESEEEGAAVMQAWNEWLGGLGPAVVDQGHP
                     TGASATVGSDGSTTQGGGANPVTGYSIISADSLDEALVFAKGSPQLASGGTIEVAETL
                     DM"
     gene            1164..1781
                     /locus_tag="VFU96_11190"
     CDS             1164..1781
                     /locus_tag="VFU96_11190"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEU5113872.1"
                     /translation="MKFRIVPTSREFFDDFSLAAQNLSECVDRFNDVLADTSQASERH
                     IAVKEVERAGDEYTSRILTRLDSTFVTPFDPEDIQRLAQGIDDVVDDIYHVSEMVVLT
                     NVTELIPEVKEQVEVLSVMASKVVELFDRFEDMRGLKPIIAEIEGLESEGNRIYRRAV
                     ARLFSGEMGPVDVIRWKDIIESLEAVFDRVEDMTNIVTTIVVKQA"
     gene            1795..>2418
                     /locus_tag="VFU96_11195"
     CDS             1795..>2418
                     /locus_tag="VFU96_11195"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEU5113873.1"
                     /translation="MEIGAIIVILVALAFDFTNGFHDAANSIATVVSTRVLSPRYAVI
                     WAAFFNFVAFLIFSTHVANTIAKDVVVQSALTVGVIFAGLMGAIAWNLLTFWLGLPTS
                     SSHALVGGMAGAAVVRGGFEVLISEGLATIGAFIVLSPLVGLAIGLLLTVIIQWVFHR
                     EKNIDRLNKGFRRGQLASAAAFSLGHGGNDAQKTMGIILAVLIAVGSL"
BASE COUNT          431 a          730 c          754 g          503 t
ORIGIN      
        1 gccatttccg ggaagggacg gtcaagccgt atcagcaaca cgacgaggac ttcatgcgag
       61 aggtcgccga aggtctcaag acgttgcccc gcatcttcct cgacttggtg ctgccccagt
      121 tgccggacgt cgaacagcgt ctcagccagg gcggccgtgt gctcgatgtg ggttgcggtg
      181 gtggatgggc ggtggtgcag atagccgaac gattcccgga tgtcacgtgt ctcggcgtgg
      241 acgtcgagcc ctattcggtg gcgttggcgc aacagctcat tctcgatcga ggtctcgccg
      301 accggtgtga ggctcgagcc atgagtgtgg atgaattgga cgaagacggc gctttcgatg
      361 ttgcgaccag ctttctggtc gtccatgaga tcgcgccgtc cctgaaggac gcggcattcg
      421 cctcgatcgc ccgagccctc aagccgggcg gctacttcct tatattcgac gaggtctatc
      481 cggtcaacga tgaggaccta cgcaggatgc cgactcgctt cgccgcactg gcacagtggt
      541 acgagctcac ctggggaaac gtggtcgaca cccgtgccgg tctcgaggag ctttgcagcc
      601 aagccggtct cgcggtcatc gacgagacgg gcttctcccg gttccacatc gtggtcgcag
      661 agaagcccgt gtagtcgacc gcctggccgg agctcgatca catgtcgagg gtttcggcga
      721 cctcgatcgt tccgccggat gccagctggg gcgagccctt ggcgaagacg agcgcctcat
      781 cgaggctgtc tgcgctgatg atcgaataac ccgtgaccgg gttggctccc ccgccctggg
      841 tggtcgaccc gtcggacccg acagtggccg atgccccggt cgggtgccct tgatcaacca
      901 ctgctgggcc aagaccgccc aaccactcat tccacgcttg catcaccgcg gccccctctt
      961 cctcggactc cggggcgcta cctccgtggt agacgaacaa gaacttggcc attgcgtctc
     1021 ctttcgcacg gccacctggt gtggcctgtc cacctggtga cgaacggggt cggcgcgttt
     1081 cgacagcgtc gccgggacaa ctgcacgagg gcttcaggct ctttcgacgt agtggcatgt
     1141 cgagaactac cgtcccgtcg cccatgaaat tcaggatcgt cccgaccagc cgggaattct
     1201 tcgacgattt ctctttggca gcgcagaacc tctcggagtg tgtggatcgg ttcaacgacg
     1261 ttctggcaga cacatcccag gcttccgagc gccacatcgc ggtgaaggag gtcgagcgag
     1321 cgggagacga atacacctcg cgaatcctca cccgcctcga ctcgactttc gtgactccat
     1381 tcgatccgga agacattcag aggctggctc aggggatcga cgatgtggtc gacgacatct
     1441 accacgtctc ggaaatggtc gtgctcacca acgtcacaga actcataccc gaggtcaagg
     1501 agcaggtgga agtcctcagc gtgatggcgt cgaaggtcgt cgagctcttt gatcgcttcg
     1561 aggacatgcg tgggctgaaa ccgataatcg cagagatcga ggggctggag agcgagggaa
     1621 atcgcatcta tcggagagcc gtcgccaggc tcttctccgg tgagatgggg ccggtcgatg
     1681 tgatccggtg gaaggacatc atcgagtcat tggaggctgt attcgaccgt gtcgaggaca
     1741 tgaccaacat cgtcaccacg atcgtcgtca agcaggcctg agggaccatc gaccttggag
     1801 atcggcgcga tcatcgtcat tctcgttgcg ctggccttcg acttcaccaa cggattccat
     1861 gacgcggcca actcgattgc cacggtggtg tcgacgcgag tcctttctcc ccgctatgcg
     1921 gtcatctggg cagcgttctt caacttcgtt gcctttctca tcttcagcac tcacgtcgcc
     1981 aacacgatcg ccaaggacgt cgtggtgcaa agcgccctca ctgtcggggt gatcttcgcc
     2041 ggcctcatgg gtgcgattgc ctggaaccta ttgacgtttt ggctgggcct ccccaccagc
     2101 tcgtctcacg ccctcgtcgg tggcatggcg ggggctgcgg tggtgcgcgg cggtttcgag
     2161 gtcctcatct ctgaaggctt ggcgactatc ggggctttca tagtcctctc tccgttggtc
     2221 ggactcgcaa tcgggctgtt gttgaccgtg atcatccaat gggtgttcca ccgggagaag
     2281 aacatcgaca ggctgaacaa gggatttcgc cgcgggcagc ttgcgtcggc ggccgcgttc
     2341 agcttgggac acggtggcaa tgacgcccag aagacgatgg ggatcatcct ggctgtcctg
     2401 atcgccgtcg ggagcctc
//
LOCUS       DASTXO010000527         3521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1837386, whole genome shotgun sequence.
ACCESSION   DASTXO010000527 DASTXO010000000
VERSION     DASTXO010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1837386"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2012)
                     /locus_tag="VFU96_11200"
     CDS             complement(<1..2012)
                     /locus_tag="VFU96_11200"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113874.1"
                     /translation="MTDRRPMTAIPPAEAGRLEFLVPDPDQRSAIADLVATGADPAGA
                     WPRVISMIEADPDLVLTSPALERACALAGASRALSQAISRRPGLLVGSTPDDSVPLRL
                     RASLATIAGDDLAGVTDMTVATTRFSDAIDDLVRDVQSRVTASIAEKHPLVVDLPFAV
                     IAMGKWGARELNYSSDIDLLFVHDISNDDESASRAAALALALAIIGALSAGGNDGPGL
                     LVDADLRPEGSIGPLSRSLESYTQYYAKWGEPWELQALLKARPVTGDPSLGDRFASMV
                     EAVIWERGLDVDSLRSIRKLKEQAEAEARPSDIKRSRGGIRDVEFSVQLLQLVHGRLA
                     PELRKRGTLEALQALSDLGFVGEDDRARLDAAYRFLRDLEHRIQLWDLRQTHELPNDP
                     QRREQLGRSLGMLTEPARQLEQRLGEVRSVVRDVHERLYFRPILDALVGSPSARLGME
                     QAALRLEALGFNDLAAAKRALEDLTAGLSRRSQAMQQVLPLMLDWLSLTPDPDLGLRQ
                     LRTVLANTPDHAALVTLLQVNPLAGERLCRLLGTSRLIGDLIDRIPEFVPRLADERML
                     TEIRPREEESNRLKGLLDSRPDPDAKIGTIRRMARRRKLRIAARDILDLADTATTLRS
                     LTDSADVAVAGALHVVTSGDPTGLCIVAMGKWGGGEPSYGSDLDLM"
     gene            2048..>3521
                     /locus_tag="VFU96_11205"
     CDS             2048..>3521
                     /locus_tag="VFU96_11205"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEU5113875.1"
                     /translation="MPLRVAGAQLNLCVGDIPGNERRIAGAIDWAEGEHADVLLLPEL
                     AINGYPPEDLVLRDDFVSAGIESLHRLASRTGSTVVVVGFVDRSDGPSRGAVDAEDRR
                     VANAAAILHEGGVVGIYHKVLLPNYGVFDEDRYFAVGQEPDRVWDLGGTRVGVSICED
                     IWLPDGPPRDQAVAGAKVLLNINASPFHKGKAREREAMIVERALAAGAAVVYLNLIGG
                     QDELVFDGCSLVVGADGTICHRSPQFEEDMFVVDVPLPGEASVVGSVTDHLEPVEEVY
                     RALETGLGDYVRKNGFEQVVIGLSGGIDSALTAVIASDALGPDAVWGVAMPSRFSSSH
                     SIDDARQLADNLGLRFDLLPIDDVFGAYLDALAPVFAGTEFGISEENLQARTRGAMLM
                     AISNKHGPMVVATGNKSEMAAGYATLYGDMVGGYAVLKDVFKTLVYDLARWRNKDGDV
                     IPQNTIDKPPSAELRPNQQDSDSLPPYEVLDEILSLYVESD"
BASE COUNT          612 a         1113 c         1179 g          617 t
ORIGIN      
        1 tacatcagat cgaggtccga gccgtacgac ggctccccgc caccccactt gcccatggcg
       61 acgatgcaca ggcccgtcgg gtcgccactg gtgacgacat gaagggcccc ggccaccgcg
      121 acgtcagcgc tgtcggtgag cgagcggagg gtggtggcgg tgtcggccag atcgaggatg
      181 tcgcgcgccg cgatcctcaa cttccggcgt cgtgccatcc tgcgaatcgt cccgatcttg
      241 gcgtcggggt cgggccgcga gtcgaggagg cccttgagac gattcgactc ctcctcacgg
      301 ggtcggatct ccgtcagcat ccgctcgtcg gcgaggcggg ggacgaactc ggggatgcgg
      361 tcgatgaggt cgccgatgag acggctggtc ccgagcagtc tgcacaaacg ctctccggcc
      421 agggggttca cctggagcag cgtgaccagt gcagcgtgat cgggggtgtt ggccaacacg
      481 gtccgaagct gcctcagccc gagatcggga tcaggcgtca gggacagcca gtccagcatc
      541 agcgggagca cctgctgcat cgcttgtgac cgtcggctca gacccgcggt caggtcttcc
      601 agtgctctct tcgctgccgc gaggtcgttg aagccaaggg cctcgagccg gagcgccgcc
      661 tgctccattc ccaggcgcgc gctcggagaa ccgaccaggg cgtccagaat cggtcggaaa
      721 tagagccgct catggacatc gcgaactacc gatctcacct ccccgagcct ctgttcgagc
      781 tggcgagccg gttcggtcag catcccgagg gaacgcccca actgctcccg gcgttgtggg
      841 tcgtttggca attcgtgggt ctggcgcagg tcccagagct gaattcgatg ctcgaggtcc
      901 cgaaggaacc gataagccgc gtcgagtcgg gctcgatcat cttcacccac gaacccgaga
      961 tcgctcagag cttggagggc ttcgagggtt cctcgcttcc gcagctcggg agcgagccgg
     1021 ccgtggacca gctggagaag ctggaccgag aactcgacat ctctgatccc gccacgtgag
     1081 cgcttgatgt ccgatggcct ggcttcggcc tccgcctgct ctttcagctt gcggatgctg
     1141 cgaagtgagt cgacatcgag ccctcgctcc cagatcacgg cctcgaccat cgatgcgaag
     1201 cggtcgccga gggaagggtc accggtgacg ggacgcgcct tgagcagcgc ctggagctcc
     1261 cagggctcac cccacttggc gtaatactgc gtataggact cgagactccg cgataggggc
     1321 ccgatcgagc cctccggccg caggtcggca tcgaccagga gccccggacc gtcatttccc
     1381 ccggcagaca gagcgccgat aatggccagg gccagggcca gggctgcggc ccggctcgcc
     1441 gattcgtcat cgttcgagat gtcatggacg aagagcaggt cgatgtcgga cgagtagttc
     1501 agctcgcggg ctccccattt ccccatcgcg atgacggcga aggggaggtc cacgacaagt
     1561 gggtgcttct ccgcgatcga ggccgtaacc cgagactgga catccctgac caggtcgtcg
     1621 atcgcatcgg agaaccgggt ggtggccacc gtcatatcgg tgacgccggc caggtcgtct
     1681 ccggcgatgg tggcgagaga ggccctgaga cgtagcggaa cagagtcatc gggggtggac
     1741 ccgaccaaca atccaggccg acgggagatc gcctgactca atgcgcgcga ggcgccggcg
     1801 agggcgcatg ctcgttccag ggcagggctg gtgaggacca gatcggggtc cgcctcgatc
     1861 atcgagatca ccctgggcca ggccccggca gggtcggctc ccgtcgccac gaggtcggcg
     1921 atggctgacc tctggtccgg gtccggtacg aggaactcga gtcttcccgc ttcggcaggg
     1981 ggaatcgcag tcattggccg tcggtcggtc acgagcgaca gcgtatgccg gggcgtatcg
     2041 tggcgttatg ccacttcgag tcgccggagc ccaattgaat ctctgcgtcg gggatatccc
     2101 cgggaacgag cggcgcattg ccggggccat cgactgggcc gagggagagc atgccgatgt
     2161 cttgcttctc cctgagctgg cgatcaacgg ctacccgccc gaggacctgg tgcttcgcga
     2221 cgacttcgtg tcagcgggca tcgagtccct ccatcgtctg gcttcacgaa ccgggtcgac
     2281 ggtggtggtc gtggggttcg tcgaccgatc ggacgggcca tcacgtggtg ccgtcgacgc
     2341 cgaggatcgt cgagtggcga atgccgcggc cattctccac gaaggcgggg tcgtcggcat
     2401 ctatcacaag gtgctgctcc cgaactacgg cgttttcgac gaggaccgct acttcgccgt
     2461 cggccaggaa cccgatcgag tctgggatct ggggggaacc cgagttgggg tgtcgatctg
     2521 cgaggacatt tggcttcccg atgggccccc acgggatcag gccgtagccg gtgccaaggt
     2581 tctcctcaac atcaacgcct cgccgtttca caagggcaag gcccgagagc gcgaggcaat
     2641 gatcgtcgag cgtgcacttg cggcgggagc agcagtggtc tacctcaacc tgatcggagg
     2701 ccaggacgag ctcgtgttcg atgggtgctc tttggtggtt ggcgccgacg gcacgatctg
     2761 ccaccgctct ccgcagttcg aggaagacat gttcgtcgtc gacgtgccgc tgcccggcga
     2821 ggcctccgtg gtcggctcgg tcacggacca tctggagccc gtggaagagg tctaccgggc
     2881 actggagacc ggtctgggcg attacgtcag gaagaacggc ttcgaacagg tggtgatcgg
     2941 cctatccggt ggcatcgact ccgccctgac cgcggtcatc gcatccgatg ccctcggtcc
     3001 ggatgctgtc tggggagtgg cgatgccgtc ccgattctcc tcgtcgcact ccatcgacga
     3061 cgcacggcag ttggcagaca atctcgggct gcgcttcgac cttctaccga tcgacgacgt
     3121 gttcggggcc tacctcgatg ccctcgcccc ggttttcgct gggaccgaat tcggcatcag
     3181 cgaggagaac ctccaagctc ggactcgggg cgcgatgctg atggcaatct ccaacaaaca
     3241 cggcccgatg gtcgtggcga ccgggaacaa gtcggagatg gcagcggggt acgcgaccct
     3301 ctatggcgac atggtcgggg gctatgcggt cctgaaagac gtgttcaaaa ccctggttta
     3361 cgacctggcc cgatggcgga acaaggacgg tgacgtgatc ccccagaaca ccatcgacaa
     3421 accgccgtca gccgaactga gaccgaacca gcaggattcg gacagtcttc caccctacga
     3481 ggtcctcgat gagatcctct ccctctacgt cgagtcggac c
//
LOCUS       DASTXO010000528         2077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_473953, whole genome shotgun sequence.
ACCESSION   DASTXO010000528 DASTXO010000000
VERSION     DASTXO010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473953"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..843)
                     /locus_tag="VFU96_11210"
     CDS             complement(142..843)
                     /locus_tag="VFU96_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012628082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEU5113876.1"
                     /translation="MARTTTADPGYPQYVDASIDVDSGLNLAASRTKPSGESRGIVVF
                     AHGSGSSRHSPRNRQVAFVLHAAGFGTLLLDLLTPTEEVVDMRTGEHRFDVQMLGNRV
                     VAAIDWLRQADDAPIGLFGASTGAAAALIAAAARPEDVVAVVSRGGRPDLAGDDLPSV
                     QAPTLLIVGGRDTTVLAMNESARAQMKAETALEVVPGATHLFEEPGALEVVSRLALDW
                     FGRFLPQERIRSSEG"
     gene            complement(821..>2077)
                     /locus_tag="VFU96_11215"
     CDS             complement(821..>2077)
                     /locus_tag="VFU96_11215"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU5113877.1"
                     /translation="ARLSHPGIVTVYDSGEDVDGPFIVQELVEGTTLAVHLAENGPLS
                     PEPVVDIISQVAAALDHAHGLGVIHRDIKPANLILEPGGRVRLADFGIARTVDDAATI
                     TASGEMVGTITYLAPEILSGDPATVASDVYSLGAVTYELFSGSPPFQAETPAAMLEAV
                     RSAEVPDLHGIAPDAMAAAVSAAMSKDPTARPETAGSLAAALIGSATLVMATAPVSPS
                     VDRGSEEPTVVTGRPPPPPVRPTQPVRKRRSPWPVLLLLLAAVVVAVAAMTNDPTGAD
                     QDTTPTTTAVPTTTTPPTTPTTTVAPTTTTIPPTTTTATTPAVTAEVVAGEISALLAA
                     MSPPEFRPRDVRQLEDRLERVMEEWEGDNRDDLRRELERAFEEVADLDESQEREELTA
                     RLIELSELMGFRVDQIGQGNGKDDDG"
BASE COUNT          346 a          664 c          714 g          353 t
ORIGIN      
        1 attcgacacc ctgggtggcg tcatcgacat gtaccacagc gaccggcccg cgttccacga
       61 gcggatacga catgcctggc gaacgctcgg gcccagggtc acggctgccc gcatgctccg
      121 cgactacgtc attgacgtct atcagccctc gctggaccga atccgttcct gaggtaggaa
      181 gcgcccgaac caatccagag cgagtcggga caccacctcg agagctcccg gctcctcgaa
      241 gagatgggtg gcaccgggta cgacctcgag ggcggtctcg gctttcattt gcgctcgggc
      301 gctctcattc atcgcaagca ccgtcgtatc ccggccgccc acaatgagca gggtgggcgc
      361 ctggaccgag ggaaggtcgt cgccggccaa atcggggcgg ccgccccggg agacaacagc
      421 aacgacgtct tcaggtcggg ccgccgctgc gatcaacgca gccgctgcac cggtcgaggc
      481 cccgaacagg ccgatcggcg cgtcatcagc ctggcggagc cagtcgatgg cagcgacaac
      541 acgattcccc agcatctgca cgtcgaatcg atgctcaccc gtccgcatgt ccaccacttc
      601 ctcagtgggg gtgagcaggt cgaggagcag ggtcccgaac cctgcggcgt ggaggacaaa
      661 ggccacttgt cggttgcgtg ggctgtgacg gctgctccca ctgccatggg cgaagacgac
      721 gatccctcga gattcgccgc tgggtttggt tcgactggcg gcgagattca atccggagtc
      781 gacatcgatg ctcgcatcga cgtactgcgg atacccggga tcagccgtcg tcgtccttgc
      841 cattgccctg cccgatttgg tcgactcgga agcccatcag ctccgagagc tcgatcaggc
      901 gagccgtgag ctcctcccgt tcctgcgatt cgtcgagatc ggcgacttcc tcgaaggccc
      961 gctccagctc gcgtctcaaa tcgtcgcggt tgtcgccctc ccactcctcc atgactcgtt
     1021 ccaggcggtc ctccagctga cggacatcgc gtggtcgaaa ctcagggggg ctcatcgcag
     1081 caaggagagc acttatctcg cccgccacga cctcggcggt caccgcgggc gtggtggccg
     1141 tggtggtggt gggcgggatg gtcgtcgtcg tcggggcgac ggtcgtagtt ggagtggtcg
     1201 ggggtgtcgt cgtggtcggg acggccgtgg tcgtgggggt ggtgtcctgg tcggcacccg
     1261 tggggtcgtt cgtcatcgcg gcgaccgcga cgacgacagc tgccagcaat aggaggagga
     1321 cgggccaggg cgaccggcgc ttgcgcactg gctgggtcgg cctcacgggt gggggcggcg
     1381 ggcgacccgt gacgacagtt ggctcctccg atccacgatc aaccgacggg ctgactgggg
     1441 ccgtggccat gacgagggtg gctgacccga tgagagcggc ggcgagagag cccgccgtct
     1501 ccggcctcgc tgtcgggtcc ttggacatgg ctgccgacac tgcggcagcc atcgcatcag
     1561 gcgcaatccc gtggaggtcg ggtacctcgg cgctgcgaac agcctcgagc atcgcggccg
     1621 gagtctcggc ctggaacggg gggcttccgg agaacagctc ataggtgact gcgccaagcg
     1681 aatagacgtc ggaggccacg gtcgccggat cgccggaaag gatctctggg gccagatagg
     1741 tgatggtgcc gaccatctca ccactcgcgg tgatcgtggc cgcgtcgtca acagtgcgtg
     1801 cgatgccgaa gtcggcgagc ctgacccgtc cacctggctc gaggatcaga ttggccggct
     1861 tgatgtcgcg atgaatcaca ccgaggccgt gggcgtgatc gagtgcagca gcaacctggg
     1921 agatgatgtc gacaacaggc tccgggctga gcggcccgtt ttcggccaga tgaacggcca
     1981 gcgtcgtccc ttccacgagt tcctggacga tgaagggccc gtcgacgtcc tcaccactgt
     2041 cgtacacggt gacgatcccc gggtgactga ggcgcgc
//
LOCUS       DASTXO010000529         3376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_953346, whole genome shotgun sequence.
ACCESSION   DASTXO010000529 DASTXO010000000
VERSION     DASTXO010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953346"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VFU96_11220"
     CDS             complement(<1..870)
                     /locus_tag="VFU96_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5113878.1"
                     /translation="MKAVDGVSFSLRQGEALGIVGESGCGKTMTALSLLRLVPRPAAR
                     IVKGRIVLEGENILEKTEREMREKIRGRRISMILQDPQTSLNPVFTIGNQLIEAIQIH
                     HRDGHRSLVRRAIDGLKQVRVAAPERRVSDYPHQISGGMKQRVVGAIAISCEPRVIIA
                     DEPTTSLDVTIQAQYLRLLRDIQEATNLALIFITHDFGIVAKMCDRVMVMYAGRVVES
                     GPVREIFNHPCHPYTQALLNSVPTMDERVERLYSITGQPPALWDLPLGCRFEARCPHA
                     DERCRLHYPPAFTV"
     gene            complement(1076..2710)
                     /locus_tag="VFU96_11225"
     CDS             complement(1076..2710)
                     /locus_tag="VFU96_11225"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5113879.1"
                     /translation="MRRSPLSWFSSPRLVPVALGLLLALALTLETTLTPSAQESKVSK
                     LVFASAGFEDSNRFWMISRPDHLQYDPFLETLLEVDPKTGEYTPRLAEKWQASPDYKE
                     WTFFLRKGVPFHNGYGQFTAKDVVHSHSLMMRQEATATLVGFWRQVEEVKAVNDHQVV
                     FRFKRPTTVMPYAASRAGDLRIVSKAQWDKEGLEGFDKKPAGTGSYRYVSRQQGLSIN
                     FERVDNHWKGEKPAFKELEFRLAREESTRLALLLSGEAQVADLPRELQKDALKKGMKI
                     FSSSFPVDWMTVYLGGQYYLPGDPAFKPDVAWTKKKVRQALNMAINRKELLNTVFAGK
                     GTMTYVTGWSSQSEGYNPEWQSRFDKMYGYDPAKAKALLKEAGYGPGQLKFKILAFTE
                     PGESEGPQVAEALGIYYKDIGVETEIEVLDWAKVREMFRKKTIQCCIWPNIISWRPSE
                     EWIRTSYYSKSPNHHYENEFIDKTYQELTNSVKPADRNRLARTIADHLFEEFADIPLF
                     WFANEVVANPKVVSEWVYPGLGAGRSTHFHLIKATK"
     gene            complement(2780..>3376)
                     /locus_tag="VFU96_11230"
     CDS             complement(2780..>3376)
                     /locus_tag="VFU96_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5113880.1"
                     /translation="VSLIAIFVGGTLGTAMGLMSGYFGGKTDSLIMRLVDISLSLPTI
                     LLALVLVAAVGPSFGTVITVLVVLLWARYARLVRGETLSIKERDFIARARVAGASHTR
                     IMLRYVFPNVVNSLVVLATLQVGYVILLESALSFLGAGLPRPTPAWGLMIADGRELIV
                     TAWWVSMFPGLAIMLTVLSLNLLGDWLRDHLDPKLRHV"
BASE COUNT          613 a         1098 c         1060 g          605 t
ORIGIN      
        1 caccgtgaac gccggaggat agtgaagccg gcaacgctca tcggcgtggg ggcaccgagc
       61 ttcgaatcga caccccagcg gtaagtccca gagggcggga ggctggccgg tgatcgaata
      121 gagccgctcg acgcgctcgt ccatggttgg caccgagttc aacaaggcct gcgtatacgg
      181 gtggcaagga tggttgaaaa tttcccgcac cgggccgctc tccacgaccc gcccggcgta
      241 catgaccatg acgcgatcgc acatcttcgc cacgatgcca aagtcgtggg tgatgaagat
      301 cagcgcgagg ttggtggcct cttgaatgtc gcgcagcagg cgcaggtatt gagcctggat
      361 ggtgacgtcc agcgaggtgg tgggctcgtc ggcgatgatg acgcggggct cgcacgagat
      421 ggcgatggcg ccgacgacgc gctgcttcat gccgccgctg atctggtgag gataatccga
      481 cacgcggcgc tcgggcgccg ccacccgcac ctgcttcaag ccatcgatcg cccgccggac
      541 cagcgaccga tgaccgtctc ggtgatggat ctggatggct tcgatgagct ggttcccgat
      601 ggtgaacacc ggattgaggg aagtctgcgg atcctgcagg atcatcgaga tccgtcgtcc
      661 ccggatcttc tcccgcatct cgcgctcggt cttctccaga atgttttcgc cctcgagcac
      721 gatccggccc ttgacgatgc gggcggccgg ccgcgggacg agacgcagga gcgacagggc
      781 cgtcatggtc ttgccgcacc ccgactcgcc cacgatgccc agcgcttcgc cctgtcgcag
      841 ggagaagctc acgccgtcga cggccttcac cacaccccac cgggtaaagc agtaggtgtg
      901 caggtcctcg acttccagaa tgacgtcacc gatctgtctg ggcatgcgcc acccgccgtc
      961 acatggagga cgtcacgaac ctcgggatat cagcggatca aggccgccgg gccctgggag
     1021 aatcgctcct cctcgacccg gcggccttca gtctcgcgcc tctcgaaccg tacttctact
     1081 tcgtggcctt gatgagatgg aagtgggtcg accgcccggc accgagcccc gggtacaccc
     1141 actcgctcac cactttcggg ttcgcaacga cctcgttggc gaaccagaag agcgggatgt
     1201 cggcgaattc ctcgaacagg tgatcggcga tcgtccgcgc caggcgattc cggtcagccg
     1261 gcttcaccga gttggtgagc tcctggtagg tcttgtcgat gaactcgttc tcgtagtggt
     1321 ggttcgggct cttgctgtaa tagctcgtcc gaatccactc ctccgacgga cgccaggaga
     1381 tgatgttggg ccagatgcag cactggatcg tcttcttgcg gaacatctcc cgcaccttgg
     1441 cccagtcgag cacttcgatc tcggtctcga cgccgatgtc cttgtagtag atgcccagcg
     1501 cctcggccac ctgcggacct tccgactcac ccggctccgt gaacgccagg atcttgaact
     1561 tcagctgccc cggcccgtag ccggcttcct tcaggagggc cttggccttc gcggggtcgt
     1621 agccgtacat cttgtcgaac cggctctgcc actccgggtt gtagccctcc gactgggacg
     1681 accagccggt cacgtacgtc atggtcccct tgccggcgaa caccgtgttg aggagctcct
     1741 tgcggttgat ggccatgttc agagcctggc gcaccttctt cttggtccag gccacgtccg
     1801 gcttgaacgc ggggtcgccg ggcaggtagt actggccgcc caggtacacc gtcatccagt
     1861 ccaccggaaa cgacgacgag aagatcttca tgcccttctt gagggcgtcc ttctgcagct
     1921 cacgcgggag gtcggcaacc tgggcctcgc cggagagcag gagggcgagc cgagtcgact
     1981 cctcgcgggc cagccggaac tccagctcct tgaacgcggg cttctcgccc ttccagtggt
     2041 tgtccacccg ctcgaagttg atggacaggc cctgctggcg gctgacgtag cgatacgagc
     2101 cggttcccgc cggcttcttg tcgaagcctt ccaggccttc cttgtcccac tgggccttgc
     2161 tgacgatccg cagatcgccc gcgcgggacg ccgcgtacgg catgacggtc gtcgggcgct
     2221 tgaagcggaa cacgacctgg tgatcgttga cggccttcac ttcctcgacc tgacgccaga
     2281 agccgaccag ggtcgcggtc gcttcctgac gcatcatgag cgagtgagaa tgcacgacgt
     2341 ccttggcggt gaactggccg tagccgttgt ggaacggaac gcccttgcgc agaaagaacg
     2401 tccactcctt gtagtcgggg ctggcctgcc acttctccgc cagccggggg gtgtactcgc
     2461 cggtcttcgg atcgacctcg aggagcgttt ccaggaaggg gtcgtactgc aaatgatccg
     2521 gacggctgat catccagaag cggttgctgt cctcgaagcc cgcggatgcg aacacgagct
     2581 tcgagacctt cgactcctgg gccgaaggtg tcagcgtggt ctccagggta agcgccaacg
     2641 ccagcagcag ccccagtgcg acgggaacga gccgcggcga cgaaaaccat gacaacgggc
     2701 tgcgtctcat cgggctatgc ctcctccgaa tttttgttct acctcgccgg cgtcggctgc
     2761 tgtcccttcc gacgctgtcc tacacgtgcc ggagcttggg atccagatga tcccggagcc
     2821 agtctcccag cagattcagg gagagcaccg tcaacatgat cgccaggccc gggaacatcg
     2881 acacccacca cgccgtgacg atgagctcgc gaccgtccgc gatcatcaga ccccaggccg
     2941 gggtgggacg gggaagccca gcgccgagaa aactcagggc cgattcgagc agaatgacgt
     3001 atccaacctg gagcgtcgcg aggacgacca gggagttgac gacgttcggg aacacgtagc
     3061 ggagcatgat gcgggtgtgg gacgccccgg cgacccgcgc cctggcgatg aaatcccgct
     3121 ccttgatgct gagagtttcc ccacggacga gccgggcgta gcgcgcccac agcagaacca
     3181 ccaggacagt gatgaccgtc ccgaagctcg ggcccaccgc ggcgaccaga accagcgcga
     3241 gcaagatcgt gggtaaggag agcgagatat cgaccaggcg catgatcagc gagtccgtct
     3301 tgcccccgaa gtagcccgac atcaacccca tggcggtacc gagggtgccg cccacgaaaa
     3361 tcgctatcag cgacac
//
LOCUS       DASTXO010000530         2494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_989765, whole genome shotgun sequence.
ACCESSION   DASTXO010000530 DASTXO010000000
VERSION     DASTXO010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989765"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /gene="rimI"
                     /locus_tag="VFU96_11235"
     CDS             complement(<1..370)
                     /gene="rimI"
                     /locus_tag="VFU96_11235"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEU5113881.1"
                     /translation="MGRGVSTMVIRPLIIDDLATVVALEAAHASSPWTEGVFRDELAA
                     ENRIYLAADEDGVVGFAGAMLVADEAHVTNLLVDPSWRRQGIGYELMKTLIEAAVAAG
                     ARHLTLEVRSANEAARALYAR"
     gene            complement(355..1065)
                     /gene="tsaB"
                     /locus_tag="VFU96_11240"
     CDS             complement(355..1065)
                     /gene="tsaB"
                     /locus_tag="VFU96_11240"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU5113882.1"
                     /translation="MRLLAIETSTPGASVALVEDATTLAAASRIDPVGHATFLVPAID
                     FCFDQVGWSPSALDAVAVDIGPGLYTGIRVGVATAQGLAAAFGIPIIPAVSTDAIALE
                     ARTGHRLIWAVVDVRRGELAVARYQPVPGGVVRTTDPELVKPDVLRAQLDSAPEDSLL
                     VGDAGALPKGLLRGLHRVKLGRPRYPYAVALAEVARGKYEKDEFPPSTEIRPMYLREP
                     DVTINWAKLREEGPWAGA"
     gene            complement(1062..1514)
                     /gene="tsaE"
                     /locus_tag="VFU96_11245"
     CDS             complement(1062..1514)
                     /gene="tsaE"
                     /locus_tag="VFU96_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006541576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU5113883.1"
                     /translation="MIEIPCRTEADTRAVARKLAATLRPGDVILLAGGLGVGKTLFTT
                     GLAAGLGIEEQVLSPSFVLVRQYRSGFLPLVHVDVYRLGSINEFDDLEALDLAIDGVL
                     VIEWGDALEGEIPDDHLLIDFVVEEDGSRTLRLQPSGNWIKRDLGLVA"
     gene            complement(1511..2143)
                     /locus_tag="VFU96_11250"
     CDS             complement(1511..2143)
                     /locus_tag="VFU96_11250"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU5113884.1"
                     /translation="MSDSIQILEELRERALVCIQCGLAETRTQVVFGVGDPQARLMFV
                     GEAPGQNEDIQGEPFVGAAGKLLDTLLGEIGLQRSEVYIANVLKCRPPGNRDPRPDEI
                     DACKGYLREQIRLIAPEVVMTLGNFATKLLLNTETGITRLRGRPHQWWLGALLIPTFH
                     PAAALRGGDRVADQMREDFALVRSILDSPAEPEPEAATTEPDPAQMELFG"
     gene            complement(2220..>2494)
                     /locus_tag="VFU96_11255"
     CDS             complement(2220..>2494)
                     /locus_tag="VFU96_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEU5113885.1"
                     /translation="PAPFPELVPNTTLVVIATDAAMTRSELSRLAVRAHDAMAVCIRP
                     VHTRFDGDIVFAVSCGGVEADVEALAEAAFGATAAAIERAVKAAAG"
BASE COUNT          451 a          847 c          816 g          380 t
ORIGIN      
        1 gacgggcata caaggcacgg gccgcctcgt tggccgagcg cacctccaga gtcagatgcc
       61 gggcgccggc cgcgaccgcc gcctcgatga gggtcttcat caattcgtag ccgattccct
      121 gccgacgcca agaaggatcg acgagcaggt tggtgacatg tgcctcgtcg gcgaccagca
      181 tcgcaccggc aaaaccgacg acgccgtcct cgtcggccgc caggtagatc cggttctccg
      241 cggcgagctc gtcccggaag acaccctcgg tccacgggga ggaggcatgc gccgcttcca
      301 gcgccaccac cgtcgcgagg tcatcgatga tgagtggtcg aatgaccatg gtgctcacgc
      361 ccctgcccac gggccctcct cacgcagctt ggcccagttg atcgtgacgt cgggctcgcg
      421 gagatacatc gggcggatct cggtggatgg agggaactcg tccttctcgt atttgccccg
      481 ggccacctcg gccaacgcca ccgcgtatgg gtaccggggc cggcccagct tcacccgatg
      541 cagcccgcgc aacaaccctt tggggagagc accggcgtcg cccaccagca ggctgtcctc
      601 gggcgcggag tcgagctgag cccgcaacac gtccggcttg accaactcgg ggtcagtggt
      661 ccgcaccacc ccgccgggaa ccggctggta gcgggccacg gccaattcac cacgtcgcac
      721 gtcgacgacc gcccagatca accggtgccc ggtccgtgcc tccaacgcga tggcatcggt
      781 ggatacggcg gggatgatcg ggatcccgaa ggcggcggcc aggccctggg cagtggccac
      841 gccgacccgg atgccggtgt acaacccggg accgatgtcg acggcaaccg cgtccagggc
      901 cgagggcgac cagccaacct ggtcgaagca gaagtcgatg gcgggcacca ggaatgtggc
      961 atggccgacc gggtcgatcc gcgacgccgc agccagggtc gtggcgtcct cgaccagcgc
     1021 caccgaagcg ccgggggtgg aggtctcgat ggcgagcagc ctcatgccac caacccgaga
     1081 tcgcgcttga tccagttgcc actcggctgg agacgaagag tgcgggatcc atcctcttcg
     1141 accacgaaat cgatgaggag atgatcatcc gggatctcgc cctccagggc atcaccccat
     1201 tcgatgacaa gcactccgtc gatggccagg tccaatgcct cgagatcgtc gaactcattg
     1261 atcgaaccga ggcgatagac gtcgacgtgg accaatggga gaaacccgct ccgatattgg
     1321 cggaccagga cgaacgaagg gctgagcacc tgctcctcga tgcccaggcc cgcggccaga
     1381 ccggtcgtga acaatgtctt gcccacgccg aggccaccgg cgagaaggat gacgtccccc
     1441 ggtcggagtg tggcggccag cttgcgggct acagcgcggg tatccgcctc ggtacgacaa
     1501 gggatctcga tcatccgaac agttccattt gagcaggatc gggttcggtc gttgcagcct
     1561 cgggctccgg ctcggcgggc gagtcgagaa tcgagcgcac cagggcgaag tcctcgcgca
     1621 tctggtcggc aacacggtcg cccccacgga gcgcggcggc cgggtggaac gtggggatga
     1681 ggagcgcccc cagccaccat tggtggggcc ggccccggag ccgggtgatc ccggtctccg
     1741 tgttgagcag gagcttggtg gcgaagttcc ccaacgtcat gaccacttca ggcgcgatca
     1801 gcctgatctg ctctcgcaga tagcccttac aggcgtcgat ctcatcgggt cgggggtcgc
     1861 gatttcccgg cggacggcat ttgaggacgt tggcgatgta cacctctgac cgctgcagcc
     1921 cgatctcacc gagcaaggta tcaagcaact tccccgcggc accgacaaac ggctcgccct
     1981 gaatgtcctc gttctgaccg ggcgcctcgc cgacgaacat gaggcgggcc tggggatcac
     2041 cgacgccgaa gacgacctgg gtccgggtct cggcgagccc gcactggatg caaacaagcg
     2101 cacgttccct cagctcttcg aggatctgga tgctgtcgct caccagcgcc cattgtggca
     2161 ctgggcaacc gggagccacc gccgcacatc tgagatttct gtcctgatgc gctgagaaat
     2221 cagccggcgg ccgccttcac cgctcgctcg atcgccgcgg ccgtcgcccc gaaggccgcc
     2281 tcggccagcg cctcgacatc ggcctcgacc ccaccgcagg agacggcgaa gacgatgtca
     2341 ccgtcgaatc gggtatggac cgggcggatg cagacggcca tggcgtcatg cgccctcact
     2401 gccaaccggc tcagctctga ccgggtcatc gccgcgtcgg tggcgatcac caccagagtc
     2461 gtgttgggga ccaactccgg gaacggggcc ggtg
//
LOCUS       DASTXO010000531         2858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1063752, whole genome shotgun sequence.
ACCESSION   DASTXO010000531 DASTXO010000000
VERSION     DASTXO010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063752"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VFU96_11260"
     CDS             <1..372
                     /locus_tag="VFU96_11260"
                     /inference="COORDINATES: protein motif:HMM:NF036800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113886.1"
                     /translation="RLLQLPVEVQEMVDGGELSAGHARALVGMEDAAYATHLAGRAVD
                     EGWSVRQMEEAVRVRKGQPRNSPTGVRQLRPVEIIELEKRLTDHLGAPVKISYRNEKG
                     KVEIKFGSLGELERLYRMFNG"
     gene            complement(362..1342)
                     /locus_tag="VFU96_11265"
     CDS             complement(362..1342)
                     /locus_tag="VFU96_11265"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEU5113887.1"
                     /translation="MRLYRVGDEGAAVKDIQERLDALGYPTAPDLAAIFGGATREAVC
                     EFQRARGLDVDGIVGPDTWRSLYEAGYRLGDRMLFLRRPMMRGEDVSEIQSRLSSLGF
                     DVGKVDGIFGPKTEEAIIEFQHNRNLAEDGKAGPEVVTEIHLVTRGEMKEGRQAVRER
                     EWLRRLPPTVAGARVYLDAGCRDTHEASVTWEAASAAALGLQELGGLPVLSRSQDGSV
                     PERLRAHRANVFGADLIVAFQLSPDGDDSVFYFASEHSRSEAGELLARSMASILGGKV
                     EGRAGALLKETRAPAVVVSRSKLDEEVGYAVVDGLTRFFANAAVEVTASR"
     gene            complement(1391..1711)
                     /gene="trxA"
                     /locus_tag="VFU96_11270"
     CDS             complement(1391..1711)
                     /gene="trxA"
                     /locus_tag="VFU96_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU5113888.1"
                     /translation="MAENIVTLNDGTFNDAISADKPILVDFWAEWCGPCRVIAPVLEE
                     IAEEHDGLTIGKLNVDHNPVPAAQHDVMSIPTLILFQDGVEKKRIVGAAPKHRLLQEL
                     AEFL"
     gene            complement(1743..2669)
                     /gene="trxB"
                     /locus_tag="VFU96_11275"
     CDS             complement(1743..2669)
                     /gene="trxB"
                     /locus_tag="VFU96_11275"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018348999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEU5113889.1"
                     /translation="MNHEKLIIIGSGPAGLTAALYAARADLEPLVFEGVAAGGQLMIT
                     TDVENYPGFPDGILGPELMDQFRKQAERFGARMQQVDVTAVDFEPRPLQVNVGADEYT
                     ADAVIVATGATAKWLGIPGEEELTGRGVSACATCDGFFFRDQELVVVGGGDTAMEEAL
                     FLTKFASKVTIVHRRDEFRASKIMAQRVLDHEKIEVLWNTVLTEIHGGTTVEKVTLED
                     TVTGEQREMSANGVFVAIGHKPNTELFAEELDLDENGYIVKSPDGSTRTDVDGVFAAG
                     DVADHTYRQAVTAAGTGCMAAIDAERWLAGLE"
     gene            complement(2676..>2858)
                     /locus_tag="VFU96_11280"
     CDS             complement(2676..>2858)
                     /locus_tag="VFU96_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113890.1"
                     /translation="KGIRMTGLGILAASLGLSALLFLFLAIFGALEIPLTTAGAFAVF
                     GVVFVGAGSYLWLKRT"
BASE COUNT          511 a          931 c          889 g          527 t
ORIGIN      
        1 cgtctcctcc aactgccagt ggaggtccag gagatggtcg atggcggtga gttgtccgca
       61 ggtcacgccc gcgccctcgt cggcatggag gacgccgcct acgcaactca cctggccggc
      121 cgcgcggtcg acgagggatg gagtgttcgc cagatggagg aggcggtgag agtgcgcaag
      181 ggccaaccgc gcaacagccc gaccggtgtc cgccagcttc ggccggtgga gatcatcgag
      241 ctcgagaagc ggctcaccga tcatctcggg gcaccggtca agatcagcta ccgcaacgaa
      301 aagggcaaag tggagatcaa gttcggttca ctgggagagc tggaacgcct ctaccggatg
      361 ttcaacggct agccgtgacc tcgacggcgg cgttggcgaa gaaccgcgtc aggccatcga
      421 cgacggcata cccaacctcc tcgtcgagct tgctccggga cacgacgact gccggggcac
      481 gagtctcctt gagaagcgca ccggcccgtc cctccacctt gccgcccaga atcgaggcca
      541 tcgaccgggc gagcagctca cccgcctcag acctgctgtg ctctgaggcg aaatagaaga
      601 ccgagtcatc gccatcgggg ctcagctgga aggcgacgat cagatcggcg ccgaacacgt
      661 tggcccggtg ggctctgagt cgctccggga cggacccatc ctgggatctc gacaagacgg
      721 gaagcccgcc gagctcctgg agccccagtg cagccgcgga cgcagcctcc caggtgacag
      781 atgcctcatg ggtatcacga catccggcgt cgagatagac gcgggccccg gcgacggtgg
      841 gggggaggcg gcggagccat tcccgctcac ggaccgcctg tctgccctct ttcatctctc
      901 cccgggtgac caggtggatc tccgtgacca cttctggccc cgcttttcca tcttctgcca
      961 ggtttcgatt gtgctggaac tcgatgatgg cctcttcggt cttgggaccg aagatcccgt
     1021 cgaccttgcc gacatcgaag ccgagcgagc tgagacgaga ctggatctcc gatacgtcct
     1081 caccccgcat catcggccgg cggagaaaga gcattcggtc accgagtcga tagccggcct
     1141 cgtagagcga acgccaggta tcggggccga ctatgccgtc cacgtccagg ccgcgagcac
     1201 gttggaactc gcacaccgct tcgcgggtcg cccccccaaa gatcgctgcg aggtctggtg
     1261 ccgtcggata gcccagggcg tcgagacgct cctggatatc cttgaccgct gctccctcgt
     1321 caccgacgcg atagagacgc atgtcgtttc acgttatccg ttgtccgggt tgcggtcgtg
     1381 tgtgcgtcgc tcagagaaac tcggcgagct cctgcaggag ccggtgcttg ggggccgcgc
     1441 cgacgattct cttcttctcg accccgtcct gaaagaggat cagggtgggg atgctcatta
     1501 cgtcgtgctg ggcggccggc accgggttgt ggtcgacgtt gagtttgccg atcgtgaggc
     1561 cgtcgtgctc ctcggcgatc tcttcgagca ccggggcgat cacccggcac ggaccgcacc
     1621 actctgccca gaagtcgacg aggatgggct tatcggcgct gatcgcgtcg ttgaaggtgc
     1681 cgtcgttcag ggtgacgatg ttctcggcca tagctttggt ctctccttga ggtgtcagag
     1741 tctcattcca ggccggccag ccatcgctcg gcgtcgatgg ctgccatgca cccggtcccc
     1801 gctgcagtga ccgcctggcg ataggtgtga tcggcgacgt ctccggcggc aaacactccg
     1861 tccacatcgg tgcgtgtgct gccgtcgggc gacttcacga tgtacccatt ctcgtccaga
     1921 tcgagttctt cagcgaataa ctccgtgttg ggcttgtgtc cgatggctac gaacacgcca
     1981 ttggccgaca tctcccgttg ctccccggtg acggtgtcct ccagggtcac tttctcgaca
     2041 gtcgtgcccc catggatctc ggtgagcacc gtgttccaca acacctcgat cttctcgtgg
     2101 tcgaggactc gttgcgccat gatcttcgaa gcgcggaact cgtcccggcg gtggacgatc
     2161 gtgaccttcg aagcgaactt ggtgaggaac agagcctcct ccatggcagt gtcaccgccg
     2221 ccgacgacga caagctcctg gtctctgaag aagaaaccgt cgcaggtggc acaggccgac
     2281 actccgcgtc cggtcagttc ctcctcacca ggaatgccca gccatttggc ggtggcgccg
     2341 gtggcgacga tcacggcatc ggcggtgtac tcgtcggcgc cgacatttac ctggagcggc
     2401 ctcggctcga agtcgactgc agtgacatcg acctgctgca tgcgggcgcc gaatcgctct
     2461 gcctgcttcc ggaactgatc catcagctcc gggccgagga tgccgtcggg gaagccgggg
     2521 tagttctcga cgtcggttgt gatcatcagc tggccacctg cggcaacacc ttcgaaaacc
     2581 agcggctcga ggtcggcgcg ggcggcgtac agggccgccg tcaaaccagc gggcccggag
     2641 ccgatgatga tcaatttctc gtgattcatt ccgtatcagg tcctcttcaa ccacagataa
     2701 ctgcccgccc cgacgaaaac gacaccgaac accgcaaagg cccccgccgt tgtgagcggt
     2761 atctcgagcg ctccgaatat cgccagaaac aggaacaaca atgcggacaa cccaaggctg
     2821 gccgccagga ttcccagccc ggtcatccgg atcccctt
//
LOCUS       DASTXO010000532         3492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1616208, whole genome shotgun sequence.
ACCESSION   DASTXO010000532 DASTXO010000000
VERSION     DASTXO010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1616208"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /gene="murB"
                     /locus_tag="VFU96_11285"
     CDS             <1..730
                     /gene="murB"
                     /locus_tag="VFU96_11285"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEU5113891.1"
                     /translation="LGRGSNLVVSDAGFDGLVIRLGQSFGGVEVDGTEVRAGGAAPLA
                     QVARTSVEAGLTGLEFFIGVPGSVGGAVRQNAGCFGMETRDRLIEASIVDLRDGIIGR
                     FGPDDLELSYRHSNVTSTRLVLGATFRSVAGDIDTGRAELRRITRWRRDNQPGGTFNA
                     GSVFKNPAGATAGEIIDSLGLKGMRVGDVAVSEKHANFFVAGPSATSEDIRKLVIAVK
                     DRVFEETGTMLEPEIQFVGFDDEN"
     gene            720..1460
                     /locus_tag="VFU96_11290"
     CDS             720..1460
                     /locus_tag="VFU96_11290"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEU5113892.1"
                     /translation="MRIDPRLAERRREVAEDRARRKVNRLVKLLIAIGVLGGVVWLFL
                     SPLLSVATVTTSGIAASSAASVLVEQGVVSGTPLILVRAGSVESALEADPWVRESVVE
                     VDWPTGVVVRIDERVPVGWLETDEGWGLYAVDGVQLAAAPEPDPLMPWIQLGPVPIDE
                     TESSPDVLGSLEFAATLPDELKAGARVRTEPGGEIWAEVTGFQVRLGRPVEMGAKALS
                     LAALLRDQPPSGSTLTLIAPTHPAVSPP"
     gene            1578..2537
                     /gene="ftsZ"
                     /locus_tag="VFU96_11295"
                     /pseudo
     CDS             1578..2537
                     /gene="ftsZ"
                     /locus_tag="VFU96_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014741447.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
     gene            2624..3247
                     /locus_tag="VFU96_11300"
     CDS             2624..3247
                     /locus_tag="VFU96_11300"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HEU5113893.1"
                     /translation="MNLPPGVDGAAFTDATDGDQRNDLTARSAVSSWLGISRSWATVR
                     QVHGASLVHAEEPGELDDADAIWSDVPGLPLAVFTADCFGVVLRAESAAGVAHAGWRG
                     ASAGVVAALREEMTRQGHEPVAAAIGPGIGPCCFEVGPDVAERFPEDVTETRWGATSV
                     DLAASLRRQLGGLEVWLSGACTMHETGFYSHRRSRTALRNATITWVA"
     gene            3244..>3492
                     /locus_tag="VFU96_11305"
     CDS             3244..>3492
                     /locus_tag="VFU96_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU5113894.1"
                     /translation="MSYEDVVMRMAAAAERVGRDPSSITLVAVSKAKPVSEIEPVYEL
                     GHRDFGENRAQEMAEKAARLPEDIRWHFVGGLQTNKARL"
BASE COUNT          596 a         1065 c         1217 g          614 t
ORIGIN      
        1 cctgggccgc ggctcgaacc ttgttgtctc cgacgccggg ttcgatgggc tggtgatccg
       61 cctcggacag tccttcggtg gcgtcgaagt tgacggaacc gaggtccgtg ccggcggtgc
      121 cgcccctctg gcacaggtgg cacgaaccag tgtggaggcc ggtctgaccg gactcgagtt
      181 cttcatcggt gtccccggct cggttggggg agcggttcgt cagaacgcgg ggtgcttcgg
      241 catggagacc cgagaccgcc tcatcgaggc ctcgatcgtg gatctgaggg atgggatcat
      301 cgggcgattc ggccccgacg acctcgagtt gtcataccgg cattcgaatg tcacctcgac
      361 ccggctggtg ttgggagcca cgttccggtc cgtcgctggt gacatcgaca caggccgtgc
      421 cgagctacgc cggatcaccc ggtggcggcg cgacaaccag ccgggaggga ccttcaacgc
      481 cgggagcgtg ttcaagaacc cggccggtgc gacggcgggg gagatcatcg actcgctcgg
      541 gttgaaaggg atgagagtcg gggatgtcgc cgtttcggag aaacacgcaa acttcttcgt
      601 ggccgggcca tccgcaactt ccgaggacat ccggaagttg gtgatcgcgg tcaaagaccg
      661 tgttttcgag gagaccggca ccatgctgga gccagagatc caattcgtcg gattcgatga
      721 tgagaattga cccacgacta gccgagcgaa gaagggaggt cgccgaggac cgggcgcgtc
      781 gcaaggtcaa ccggctggtc aagctcctca tcgcgatcgg ggtgctcgga ggcgtggtgt
      841 ggctattcct ctcaccgctg ctcagtgtcg ccaccgtgac cacatcgggc atcgccgcca
      901 gctcggcggc gagcgtcctc gtcgagcagg gcgtggtctc tggtaccccg ctcatactgg
      961 tacgggccgg gagcgtggaa agcgccctcg aggccgaccc atgggttcgt gagtcggtcg
     1021 tcgaggtcga ctggcctaca ggtgtcgtcg tgcgaatcga cgagagggtc ccggtcgggt
     1081 ggctcgagac cgacgaaggg tgggggctct acgcggtgga tggcgtccag ctggccgccg
     1141 cccccgagcc cgatccattg atgccgtgga tccaattggg gccggtcccc atcgacgaga
     1201 ccgaatcctc ccctgacgtt ctcggatcgc tcgaattcgc cgccacgctg cccgacgagc
     1261 tgaaggccgg agccagggtg cgaaccgagc ccgggggaga gatctgggcc gaggtgaccg
     1321 ggtttcaggt gaggctggga cgacctgtgg agatgggggc caaggcgttg agtctggccg
     1381 cgttgctgcg tgatcaacct ccctccggct ctactttgac cctgatcgcc ccgacccatc
     1441 ccgcggtgtc cccaccatga ttcctgcttg cgccaggtgt agaggtctag ttgagggtta
     1501 gagattgtgg ctttactaac cagtcggcga acggtaaacc tctactttag cttccgatat
     1561 ccactggagg aattgtcgtg aactcgtcga gcggcggcta tctggccgtc atcaaagtcg
     1621 tagggatcgg cggtggtggc gtcaacgccg tcaaccgcat ggtcgaagcg ggcatacgcg
     1681 gtgtcgagtt catcgcgttg aacaccgacg cccaggcact gctcatgagc gatgccgatg
     1741 tcaagctcga catcgggcgc gaagtgacaa ggggtctggg ggccggggcc aaccccgaga
     1801 tcggccgcgc cgccgccgaa gagcacaagg cggagctgga ggatgtcctc aagggctccg
     1861 acatgatctt catcacggcc ggcgaaggtg gtgggaccgg gaccggtggc gcgcccgtcg
     1921 tggccgaagt cgcccgttcc gttggggctc tcaccgtggg tgtcgtcacc aggcccttcg
     1981 gtttcgaagg gagacgacga gcggtccacg ccgaacaggg catcgatgac ctgaaggatg
     2041 cggttgacac tctgatcgtg atccccaatg acaggctgct cgaggtcggc gaccccgagg
     2101 ccccgatcgc cgagaccttc cgtcttgccg atgaggtcct catgagtggt gtcgccggca
     2161 tcaccgacct catcacgacc ccagggctga tcaacgtcga ctttgccgac gtgcggacca
     2221 tcatgtcgga cgccggttct gcggtaatgg gcatcggccg ggccaccggt gagaaccgcg
     2281 ccaagcaggc cgcggagcgc gccatatcca gtccgatgtt ggagacctcg atggaggggg
     2341 ccaaaggtgt cctcctctcg gtgtccggcc cgtcctcgat gacgctgcac gaggccaccg
     2401 aggcggctcg ggtcgtcgcc gatcacgccg acaccgaggc agacatcatc ttcggtgcca
     2461 tcgtggacga cgatctcggt gaggagatga aggtcaccgt gatcgctgcc gggttcgagc
     2521 ggagacgaca gggccggccc gcgacctcgc ggccatccac tcggtcgtat tccgggttcg
     2581 acgaggggga cgacgtcctc gacatcccaa ccttcgttca gggatgaacc tgcccccggg
     2641 tgtcgacggg gcggccttca ccgatgcaac cgacggagat caacgaaacg acttgacggc
     2701 acggtccgcg gtgtcgtcct ggttggggat ctcccgcagt tgggccacgg tcaggcaggt
     2761 ccacggggca tccctggtcc atgccgagga accaggtgag ctcgatgacg ccgatgccat
     2821 atggagcgac gtgccgggtc tgccgctcgc ggtgttcaca gccgactgct tcggcgttgt
     2881 cctcagggca gagtcggcgg ccggcgtggc ccacgccggt tggcgcgggg catcggcggg
     2941 ggtggttgcc gctctccgcg aggaaatgac tcgacagggt catgagccgg ttgccgccgc
     3001 catcggaccg ggtatcggtc cctgttgttt cgaggtaggg cccgacgtcg ccgaacggtt
     3061 tcccgaggac gtcacggaga cgcgatgggg agcgacctcg gtggatctcg ccgcatcgtt
     3121 gaggagacaa ttgggaggcc tcgaagtctg gctctccggt gcctgcacca tgcacgagac
     3181 gggtttctac agtcaccgcc ggagccgcac cgcgctccgc aacgccacca tcacctgggt
     3241 ggcatgagct acgaagacgt cgtgatgcgg atggcggcgg cagcggagcg cgtcggtcgg
     3301 gacccatcct cgatcacatt ggtggcagtg tccaaggcga agccggtctc cgagatcgag
     3361 cccgtctacg agttgggcca tcgcgacttc ggcgagaacc gggctcagga gatggccgag
     3421 aaggcggcga ggctgcccga agacattcga tggcatttcg tcgggggcct gcagaccaac
     3481 aaggcccgct tg
//
LOCUS       DASTXO010000533         3961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_622128, whole genome shotgun sequence.
ACCESSION   DASTXO010000533 DASTXO010000000
VERSION     DASTXO010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622128"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VFU96_11310"
     CDS             complement(<1..1041)
                     /locus_tag="VFU96_11310"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5113895.1"
                     /translation="MATRRGALALILMISLVAACAGGDSEGGDTTVGGTDGATTTAGE
                     TTETTAATGTTAGGEELPTLHIGIPLPFTGVVAESAEEMRQTFEMYVEQQGGTLGGLP
                     VELTFEDTETDAELVVTKTRKLIDEDQVHLLSGPMLAFEGLAMLDSVNQAGIALVGQT
                     GVAADDYTRLETPTLTAAAKHTASQETMPLGTYAYDTLGYRTVATLGQDYAWGYQTVG
                     GFQYAFEAAGGDVVQKLWAPIGTTDFAPFVNDINQDVDAVYVTLIGADIPRFVQAYSD
                     FGLKENIPMLTSEDLVAQDAIRFYSGDPAVGIVGITPFTANLDRPEMQAFVEAYQELT
                     GNIPTFWGESSYV"
     gene            1178..2350
                     /locus_tag="VFU96_11315"
     CDS             1178..2350
                     /locus_tag="VFU96_11315"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEU5113896.1"
                     /translation="MAITKQELASRHERVRSVLREGEFDALIAYSTAKVAANVRYLTD
                     YFVRFAGMQNMREGGYHMFGSCGVLFPAEGEPMVRTDQPWDIARAREISMFADTEYAG
                     HFAQDFGPVIRDRGYRRVAIDNWYIFPAREFIALTEIAPEVEFVPSHLMSEVRRIKSE
                     AELEIIRRSAALAVEAVETSLDAVRVGASEYDVVLLCEETMRRGGDIELAGGSISGCG
                     PNTSSGSWVPSRTQSRAIEAGEWVMMDVCPRVDGYAGDISRHRVAGDLSDLDPRLQKM
                     YDVALLMSEEVRKAIKPGVTGRDLNSLAESIAEGEGLLENKIGLLGHGLGLDIHDIPD
                     FYYDDSAISPGEVITVEPCLLLEGVGGVRIEDMVLVTEGGSETLTSGADRELRGSS"
     gene            complement(2347..3456)
                     /locus_tag="VFU96_11320"
     CDS             complement(2347..3456)
                     /locus_tag="VFU96_11320"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5113897.1"
                     /translation="MSDGYGYGIIGCGWVASAHAWGVQSLAGDGVRLIGVADQDLARA
                     GELGERFGAPVVTSDYRELLANDAVHVVSICLPDYLHHEVAIAAAGAGKHILCEKPLS
                     ITVDEADDMLSACEANGVQLGLIMNHRYAPGNLLARRVVASERLGRPLIASVLHASGL
                     SGDPRGPSPWRGRRRLSMGGVLSTQAIHFLDILLWLGGPVASVQAWTDRLESPDQEHE
                     DTVGVALRLVSGALATLTATSASPIMDDFTGTRIEMHGTSGYVILDGDVLRVADVADP
                     PDLAPPPLPEIPSGSEEIVFGSGHVYEVMDFVRSVRRGGAAPIPGTDGRHLIAVIDAA
                     YRSARNGEAVDVDEPTSAYTRAAPAESLLGSAVAG"
     gene            3644..>3961
                     /locus_tag="VFU96_11325"
     CDS             3644..>3961
                     /locus_tag="VFU96_11325"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEU5113898.1"
                     /translation="MTDPERRIGVIGLGNMGGALATSLLRAGHPTCGFDIERNRIEHF
                     EAEGGNAMSSPAEVAASCNTVITSLPSVRALQDVIGGGEGLATQGRDALVVIETSTLP
                     LDAK"
BASE COUNT          739 a         1266 c         1261 g          695 t
ORIGIN      
        1 cacgtaggac gactctcccc agaaggtcgg gatgttgccg gtcagctctt ggtatgcctc
       61 gacgaaagcc tgcatctccg gccggtcgag gttggctgtg aacggggtga tcccgacgat
      121 cccgaccgct gggtctccgg agtagaacct gatggcgtcc tgggcaacca ggtcctccga
      181 cgtcagcatc gggatgttct ccttcaggcc gaagtcggaa taggcctgaa cgaaccgtgg
      241 gatgtcggct ccgatcaagg tcacatagac ggcatcgaca tcctggttga tgtcgttcac
      301 gaacggtgca aagtcggtcg tcccgatcgg tgcccacagc ttctggacga cgtccccacc
      361 agcggcctcg aaggcgtatt ggaagccacc gaccgtttgg taaccccagg cgtagtcctg
      421 cccgagagtg gcgaccgttc gatacccgag cgtgtcgtag gcgtaggtcc cgagcggcat
      481 cgtctcctgg ctggccgtgt gcttggccgc agcggtcagc gtcggagtct ccagacgggt
      541 gtaatcgtcg gccgcgaccc ccgtctgacc aactagtgct atccccgcct ggttgacaga
      601 gtcgagcatg gccagaccct cgaaggccag catcgggccc gacaagaggt ggacctgatc
      661 ctcgtcgatg agcttccgcg tcttggtgac gaccagctct gcgtccgtct cggtgtcctc
      721 gaaggtcagc tcgaccggga gacccccgag ggtgccgccc tgctgctcga catacatctc
      781 gaatgtttgc cgcatctctt cggcggattc cgccacgacc ccggtgaaag gcaacgggat
      841 gccgatgtgc aaggtgggaa gctcctcacc accagcagtg gttccggtgg cggcggttgt
      901 ctccgtcgtc tcgccggcag tggtggtcgc accatcagtc ccgccgacgg tggtatcgcc
      961 gccttcggaa tcacccccgg cgcatgccgc caccaaggaa atcatgagaa tgagcgccaa
     1021 cgccccacgc ctcgtcgcca tgaaggcctc cttctagtga gagggacgaa ccctacatac
     1081 tgtagagtac aagatctcgc caggtcatgc cgagcagttg ccggggattg tgttgcgggc
     1141 ctgggatgga cactcgtgaa ccgggaggag cgcagtcatg gcgatcacga agcaggagtt
     1201 ggcgagtcga cacgagcgcg tgagatcggt gcttcgcgag ggcgagttcg atgcgctcat
     1261 cgcctactcc accgccaagg tcgcggccaa tgtccgatac ctgaccgact acttcgtccg
     1321 gttcgcgggc atgcagaaca tgcgagaggg cgggtaccac atgttcggtt cctgcggcgt
     1381 cctctttccc gccgaaggcg aaccgatggt tcgcacggat cagccgtggg acatcgcccg
     1441 ggccagagag atctcgatgt ttgccgatac ggagtacgcc ggtcacttcg cccaggactt
     1501 cggcccggtg atccgggacc gtgggtaccg gcgagtggca atcgacaact ggtacatctt
     1561 cccggcacgc gagttcatcg cactcaccga gatcgcgcct gaagtcgaat tcgttccttc
     1621 acatctgatg tccgaagtgc gtcgcatcaa gtccgaagcg gaactcgaga tcatccggag
     1681 gtcggctgcc ctcgccgtcg aggccgtcga gacctcgctc gacgcggtgc gcgtcggggc
     1741 gagcgagtat gacgtcgttc tcctctgcga agagacgatg cgacggggag gcgacatcga
     1801 gctcgccggc gggtcgatat ctggatgtgg ccccaacact tccagcggga gctgggttcc
     1861 ttcgcgcacg cagagcaggg cgatcgaagc cggcgagtgg gtcatgatgg atgtttgccc
     1921 ccgcgtcgac ggctacgcag gcgacatctc ccggcatcgc gtggcaggcg acctgagcga
     1981 cctggacccg aggttgcaga agatgtacga cgtcgctctg ctcatgagtg aagaggtccg
     2041 caaggcaatc aagcccggtg tgacgggccg tgatctgaac tctctcgcgg aaagtatcgc
     2101 cgagggtgag gggctcctcg agaacaagat cggcctgctc ggtcatggcc tcggtctgga
     2161 catccacgac atccccgact tctactacga cgacagcgcc atttccccgg gagaagtcat
     2221 caccgtcgag ccatgcctcc ttctcgaagg tgtcggcgga gttcgcatcg aggacatggt
     2281 gctcgtcacg gagggcgggt ccgagacttt gacgagtgga gcagaccgcg agctccgtgg
     2341 gtctagctaa ccggcgaccg ccgatccgag cagcgactcc gcaggggcgg ctctcgtgta
     2401 cgcgctcgtc ggttcatcga catcgactgc ttcgccgttc ctggctgacc ggtaagcggc
     2461 atcgatgacc gcgatgaggt gccgtccatc ggtccccggt attggagcgg ctcctccccg
     2521 ccgcacggaa cgcacaaaat ccatcacttc gtagacgtgc ccagaaccga acacgatctc
     2581 ctcagagcct gatgggatct ctggcagcgg tggcggggcg aggtcgggag ggtcggccac
     2641 gtcggcgacg cgcagcacgt caccatcgag gatcacgtac ccactcgtgc catgcatctc
     2701 tatccgcgtt cccgtgaagt catccattat cgggctggcg ctcgtggccg tcagagtcgc
     2761 gagcgctccg gacaccaacc tcagggcgac gcccaccgtg tcctcgtgct cctgatcggg
     2821 ggactcgaga cggtcggtcc aggcctggac cgaggccacc gggccaccga gccacaacag
     2881 gatgtcgagg aagtggatcg cctgcgtgct caggacgcca cccatcgaga ggcggcggcg
     2941 acctcgccac ggtgaagggc cgcgcggatc gccgctcaat ccgcttgcgt gcaatacgct
     3001 cgcgatcaac ggccttccga gtctctccga cgcgacaacc ctccttgcca ggagattccc
     3061 cggggcatat cgatggttca tgatcaagcc cagctgaacg ccgttcgcct cacacgccga
     3121 gagcatgtcg tcggcctcat cgacggtgat cgagagcggc ttctcgcaca ggatgtgctt
     3181 tccagcaccg gcagcggcga tcgcgacctc gtgatgcagg taatcgggga ggcagatcga
     3241 cacgacatgg accgcgtcat tggcgagcag ttcccgatag tcggatgtca ccaccggcgc
     3301 accgaaccgt tcgccgagct ccccggcgcg cgctagatcc tgatcagcaa ccccaatcag
     3361 acgcacaccg tcgccggcga gactctgaac gccccacgcg tgcgccgacg cgacccaccc
     3421 gcacccgatg atgccatagc catacccatc actcatctgc cacgaccacc tggatcatcc
     3481 ggaagcggcc cgcagcatac gcacggagtc accagcctga gcacggctcc gcgtgcacgc
     3541 agaatcatcg atctcgagat ctacgatgcg ggtctcgtga ggaaaatcgg tcgatgaacg
     3601 aggccaccac cggctcgacg gtttggggag gagacatcat ccagtgacgg atccagagcg
     3661 gcgtatcggg gtcatcgggc tcgggaacat gggtggggcg ctcgccacga gccttcttcg
     3721 ggcgggtcat cccacgtgcg ggttcgacat cgagcggaac cgaatcgagc acttcgaggc
     3781 agagggcggg aacgccatga gctcgcccgc tgaggttgcg gcgagctgca acaccgtcat
     3841 cacctcactc ccgtcggtta gggccctgca ggatgtcatc ggtggaggag aaggcctggc
     3901 gacccaaggg cgagacgcct tggtcgtgat cgagacgagc acgttgccgc tcgatgcgaa
     3961 g
//
LOCUS       DASTXO010000534         2351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_69154, whole genome shotgun sequence.
ACCESSION   DASTXO010000534 DASTXO010000000
VERSION     DASTXO010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69154"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1494
                     /locus_tag="VFU96_11330"
     CDS             <1..1494
                     /locus_tag="VFU96_11330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5113899.1"
                     /translation="LITDVAIYEIVIASLILLALAVVGMTAKTQGMAAGAVSLGLVTS
                     AGIIVRYLDGSIESQFAFLLALVAVSFYEEWRLVLAGLVYVAGFQIFNFLVMYREAYV
                     QRYGVAHLGLPLVFLAMTFVLVGLLIAGWRLSRQEDGRAAGEDGFRAGFEKVDIGMAL
                     LTPSGEFIHANEALVRMFGPVAGSNIRGLIHADDLADLGQAWEDMGNGDGQTATTWMR
                     WRASDGRAIWGRLSLSFLRSARQRPATILLQLEDSTRAHHDELRLERAIQGRDEFVAA
                     IAEDIRVPVGAVLDLTAQAEGDPVDLHLTVRRIESHAREVASIVDDLFISARAGNAPL
                     QPLSRAVDAVVLCREALSEVPGAERIPIEAGATSLWADPGMTIRILDSLVANAIRYGG
                     SMVSLETAGSGPDTVISVIDDGPAIPIPERERIFNGDLRAGGPVTRPAAVGLSLTVAR
                     QLARQMDGDITYRRTGDGRNVFELRLPSESLRAGQGAWEEEPVRIPA"
     gene            complement(1496..2083)
                     /locus_tag="VFU96_11335"
     CDS             complement(1496..2083)
                     /locus_tag="VFU96_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEU5113900.1"
                     /translation="MGSAPSEQTGLTPVKIVVAGGFGVGKTTFVGAISEIEPLRSEAM
                     ITDASAGIDELYPDGAKTETTVAMDFGRISLKHRLVLYLFGTPGQDRFHFMWDELARG
                     AIGAVVLVDTRRIDACFAAIDFFEKRGTPFVVAVNTFYGRRIHEPELVREALRVGPDI
                     PIVYCDARVRVDVQRALVAVVEHSLERVMTEAAAS"
     gene            complement(2064..>2351)
                     /locus_tag="VFU96_11340"
     CDS             complement(2064..>2351)
                     /locus_tag="VFU96_11340"
                     /inference="COORDINATES: protein motif:HMM:NF017170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF742 domain-containing protein"
                     /protein_id="HEU5113901.1"
                     /translation="ALEALVAVTPAGMRMRGRFRWEAAEIIALSRKEIAIVELAAMLE
                     VPVGVVRVLAVDLRDQGAITITEPPIETISESADGDYADLLHKVLDGIRAL"
BASE COUNT          404 a          769 c          740 g          438 t
ORIGIN      
        1 ctgatcacgg acgtggcgat ctacgagatc gtgatcgctt cgttgatcct cctcgcgctg
       61 gcagtggttg gcatgaccgc gaagactcag ggcatggcgg ccggcgcagt gtctcttggc
      121 ctcgtcacct cggcaggaat catcgtccgc tacctggacg gctccatcga atcccagttc
      181 gccttcctgc ttgctctcgt cgcagtttcc ttttatgagg agtggcggct ggtcctagcc
      241 gggttggtgt atgtcgccgg attccagatc ttcaatttcc tcgtgatgta tcgggaggca
      301 tacgtccagc gctacggggt ggcgcatctc gggttgcctc tggtgttcct cgcgatgacc
      361 tttgtcctgg tgggtctgct catcgccggc tggcgtctga gccggcagga ggacggccgc
      421 gccgccggtg aagacgggtt cagggcgggt ttcgagaagg tggacatcgg aatggcgctc
      481 ctgacccctt cgggtgagtt catccatgcc aacgaggcgc tcgtcagaat gttcgggccg
      541 gtagccgggt cgaacatcag aggtctcatc catgccgacg acctcgccga cttgggccag
      601 gcctgggagg acatgggcaa tggcgatggc cagaccgcca ctacttggat gcggtggcgg
      661 gcgtccgacg gccgagccat ctggggccgg ctctccctct cgttcctgcg ctcggccaga
      721 caacgcccgg ccacgatcct tctccaactg gaagattcaa cccgcgctca tcacgacgaa
      781 ctgcgtctcg agcgcgcgat ccaaggaagg gacgagttcg tcgccgccat cgccgaggac
      841 atccgcgtgc cggtcggcgc cgtgctcgat ctgacagccc aggccgaggg cgatcccgta
      901 gatctgcatc tcaccgttcg ccgcatcgaa tcccatgcgc gggaagtggc ctcgatcgtc
      961 gacgacctat tcatctccgc ccgggcaggc aacgccccgc tccaaccgtt gagccgagcg
     1021 gtcgacgccg tcgtcctgtg cagggaagct ctatccgagg ttcctggcgc cgaacgcatc
     1081 ccgatcgagg ctggagcgac ttcgctttgg gcggaccctg ggatgacgat caggatcctg
     1141 gacagtctcg tcgccaacgc gatcagatat ggcggatcga tggtgagcct cgagaccgcc
     1201 ggttcaggcc ccgacacagt catctccgtc atcgacgacg ggccggcgat accgataccc
     1261 gagcgtgaga ggatcttcaa cggcgacctg cgcgccggcg ggccggtgac gcggccggct
     1321 gcggtgggtc tcagtctcac cgtggcccga caactggccc gtcagatgga cggggacatc
     1381 acctaccgcc gaacaggcga cggccgcaac gttttcgaac tgcgtcttcc gtcggagtcg
     1441 ctcagagccg gccagggtgc gtgggaggaa gagccggtcc gcatcccggc ctgaatcagc
     1501 ttgccgcagc ctcggtcatg actcgctcca ggctgtgctc gaccacggcg acgagcgctc
     1561 tctggacatc gacgcggacc ctcgcatcgc aatacacgat cgggatatca ggaccgacgc
     1621 gcagggcttc ccgcaccagt tccggctcat ggattcgacg cccgtagaag gtgttcactg
     1681 ccaccacgaa cggcgtccct ctcttctcga agaaatcgat cgccgcgaag caagcgtcga
     1741 ttcggcgggt atcgaccagc accacggcgc cgatggcgcc tcgagccaac tcatcccaca
     1801 tgaaatggaa gcggtcctgt cccggagtgc cgaacaaata gaggaccaag cgatgcttga
     1861 ggcttatccg gccgaaatcc atcgccaccg tggtctcggt cttggcgccg tcggggtaca
     1921 actcgtcgat cccagcgctc gcatcggtga tcatcgcctc actccgcaaa ggctcgatct
     1981 cggatatggc gcccacgaac gtggtcttgc caacgccaaa gccgccggcg acgacgatct
     2041 tgaccggggt caacccggtt tgttcagagg gcgcggatcc catcgagcac cttgtgtagg
     2101 agatctgcgt agtcgccgtc ggccgattcg gaaatcgtct cgatcggcgg ctcggtgatc
     2161 gttatcgctc cctggtctcg gagatcgacg gcgaggactc tcaccacgcc gacgggaacc
     2221 tcgagcatgg ccgccagttc gacgatggcg atctccttgc ggcttagggc gatgatctcg
     2281 gccgcttccc agcggaaccg gccccgcatc cgcatcccgg cgggcgtcac tgcaaccagg
     2341 gcttcgagag c
//
LOCUS       DASTXO010000535         6098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_658856, whole genome shotgun sequence.
ACCESSION   DASTXO010000535 DASTXO010000000
VERSION     DASTXO010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6098
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658856"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFU96_11345"
     CDS             complement(<1..579)
                     /locus_tag="VFU96_11345"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEU5113902.1"
                     /translation="MDAFMTGVAAGYGIAIPVGAIAILIMDVGIRRGFAPAFSAGAGA
                     ATADLLYAALAVIGGATLAATVESIGDPLRIASGIVLLVIAVLGLLRSRHPTETNVTE
                     PRSGDLVRTYARFVGLTIINPTTVVYFAAVVIGLGVARGMGAGDGALFVTGAFLASLS
                     WQTLIAAVGAFAGHRLSSRSRRVVSILGNLVIL"
     gene            726..1973
                     /locus_tag="VFU96_11350"
     CDS             726..1973
                     /locus_tag="VFU96_11350"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5113903.1"
                     /translation="MWADRDVRRVVLARFISRTGGEAAFFVGIWGKAAFELEASAAGV
                     AAVMAALGVASLIGSAVAGVLVDRYDPRRVLIAGEIVFIPVALGMVFADTLPAMVVST
                     FLLGLVGTPVFTAISSFGPFLTDDPDRLARINGLIEGAAWAAFVVGPALGAIIAGTVG
                     LDGIFVLDAATSAIAALIVIPVSVRAMARTERDKEGLRELREGFTYAYRKHRLRFYIW
                     LGSSVWVLFGTFSALEPLFYRDILQVEPATLGWVNSIFGLGLVAGTWVASRLPPKYRT
                     ARSLVLIVALNSVGVLAYIGTDQLRVVVVAGVFWGLLIGIMAPLLRTLIQLNTPEDLM
                     GRITGVTQVHSEVGHLLPLAIAPVLAGMFGVQRTLIYAGLALAVLALFFVPTANRLDR
                     TRSVEVPPTGLPDPNDEPRSVGH"
     gene            2015..2860
                     /locus_tag="VFU96_11355"
     CDS             2015..2860
                     /locus_tag="VFU96_11355"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5113904.1"
                     /translation="MGGANRKVARAGISIVAVIVVFVLVWEGYKLIWTALGWENPVRP
                     DDRTMPHTWDIFLALFRPAQRNGPPFIIVELRAALMTLREAFVGFLIGGAVGFGLGVL
                     FVRSTVSERAFMPYVVASQTVPIIAIAPMVVVWGGRADLPQWLSVSLIAAYLTFFPVA
                     INTLRGLRSPPPTSMELMRSYASSPGEVLWKLQVPAAMPYIFTALKVSAAASVIGAIV
                     GELPAGLRGGLGRDLLQFSQQFTAAPEKLFAAVMIAGLAGIAFVGLITALEHRWIGAH
                     RRLAS"
     gene            2857..3645
                     /locus_tag="VFU96_11360"
     CDS             2857..3645
                     /locus_tag="VFU96_11360"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013653034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5113905.1"
                     /translation="MSAPVVDAQGIGKVFGSGDSQTEALRGIDLQIADGDFVSLIGPS
                     GCGKSTLLRLIGDLVSPTTGTLVVNGKEPGQARLDRDYGIVFQSATLLDWRSVQKNVE
                     LPLEIMDFPDNERPIRALEMLDLVQLGAFADHYPWQLSGGMQQRVAIARALAFRPALL
                     LMDEPFGALDEMTREAMQQEVLRVWGETGTSVVFVTHSIPEAVFLSTRVVVMSPRPGR
                     ISEEIMVDLPPARDQDTREDPRYFELVIEVREALRRGESKSGKS"
     gene            3642..4511
                     /locus_tag="VFU96_11365"
     CDS             3642..4511
                     /locus_tag="VFU96_11365"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5113906.1"
                     /translation="MTERDDLIQETSTTDVFAAFEGDLPAVPRRSSLRRLSRRYLPAV
                     LAFVAVLVVWEGLTRLFGVESFVLSKPSEILNSFAETSDVIWGAGMRTLFEAVGGFVI
                     GTSLAVATSLAAAHWVGFRQGVLPLAIALNATPIAALAPIFNNWFGLTNPFSKMAVVA
                     VVVYFPVMINTTRGLLEVDPAELELMRSLAASPAEVTRRIRIPGALPYFFASLKVASA
                     LALIAAIIAEYFGGPQDVLGQYIVNRAQLFQFPDAWAAILVASILGVVSYVLILAAER
                     WLMPWHVSFRSND"
     gene            4583..5719
                     /locus_tag="VFU96_11370"
     CDS             4583..5719
                     /locus_tag="VFU96_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5113907.1"
                     /translation="MNTSKWRRYVLLLSALAIVVAACGDGGETGGGGTGTTAPAEECT
                     EPDQVRLQLQWFAQSQFAGYYVARDNGFYEAQCLEVEIIEGAVEIVPQQVLATGGAEF
                     GLAWVPKALVSREGGADIVNIAQVFERSGTLQVSWADAGIEEPADWAGKKVGNWGFGN
                     EFELLAAIAKYEVPDVELVAQDFTMNALLNNEIDAAEAMIYNEYAQVLEAVNPDTGEL
                     YTPDDLSVIDYNEIETAMLQDAVWVNADWIAEEGNDDVATRFLTASFQGWIHCLDNFD
                     ECVEVVLNNGSTLGQSHQEWQLNEILGLIFPATNGIGVMNEDLWDQTVAIATEQIAEL
                     QGVEIPDEAYRTDLAQAAVDALGDADVTGEAFERRDIELQEGGS"
     gene            5818..>6098
                     /locus_tag="VFU96_11375"
     CDS             5818..>6098
                     /locus_tag="VFU96_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008737963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEU5113908.1"
                     /translation="MPNIVRAAIVQTEWTGDKDSMIKKNADYAREAAQQGAQVMCFQE
                     LFYGPYFCQVQENEHFEYAEPIPDGPTTQLMQDLAKETGMVLVVPIFEK"
BASE COUNT         1021 a         1949 c         1986 g         1142 t
ORIGIN      
        1 gaggatcacg agattgccca ggatgctcac caccctccgt gatcgggacg agagccggtg
       61 acccgcgaat gcgcccaccg ccgcgatcag tgtctgccag gagagacttg ccaggaaggc
      121 gccggttacg aacagtgcac catcgcccgc acccatccct ctcgccaccc cgaggccgat
      181 cacgactgcg gcgaaataga ccaccgtcgt cgggttgatg atcgtgagac caacgaatcg
      241 ggcataggtc cgcacgagat caccactccg gggctctgtc acgtttgtct cagtcgggtg
      301 tctactccgg aggaggccca gcactgcgat gacaaggagg acgatgccgc tggcgattcg
      361 caacgggtcg ccgatcgatt ccaccgtggc ggcaagggtc gctcctccga tcacggccag
      421 ggccgcatag aggaggtccg cggtcgccgc cccggcgccg gccgagaacg caggggcaaa
      481 cccgcgtctg atcccgacat ccatgatcag gatggcgata gccccgaccg ggatggcgat
      541 gccgtatccg gccgccacgc cggtcatgaa ggcgtccatc gctgtgggaa tctagagaga
      601 ggggagaggg ctcccgcaca ggtggcaccg gccgaatcga gctgatctct ttcacccccg
      661 gcggctagcc ccgccgacga gaaatcgggg aggggcgtaa ccggccccgg gtaccgtcat
      721 ccgtcatgtg ggccgatcgc gatgtcagac gggtggtcct cgcccgcttc atctcccgca
      781 ccgggggaga ggctgcattc ttcgtcggca tctggggcaa agccgccttc gaattggaag
      841 cctcggctgc cggggtcgcc gcggtcatgg cggccctcgg cgtcgcctcg ctgatcggct
      901 cggccgtggc cggggtcctg gtcgatcgct acgacccacg tcgcgttctc atcgccggcg
      961 agatcgtgtt catcccggtg gccttgggga tggtcttcgc cgacaccctt cccgcaatgg
     1021 tggtttccac gttcctcctc ggtctcgtcg ggactccggt gttcaccgcc atctcatcat
     1081 tcgggccctt cctcaccgac gatcccgatc ggctggcgcg catcaacggg ctcatcgagg
     1141 gcgccgcctg ggcggcgttc gtggtggggc cggccctcgg agccatcatc gccggcacgg
     1201 tgggcctcga tggcatcttc gtcctcgatg cggccacttc ggcgattgcc gctctgatcg
     1261 tgatcccggt ttctgtgcgg gcgatggcga ggacggagag ggataaggag ggcctgcgcg
     1321 agctgcgcga gggcttcacc tacgcctata ggaagcatcg gcttcgcttc tacatctggc
     1381 tcggttcttc ggtgtgggtc ctgttcggga ccttcagcgc gttggagccg ttgttctacc
     1441 gggacatcct ccaggtggag ccggccacgc tggggtgggt caactcgatc ttcggcctcg
     1501 gtctggtggc gggcacctgg gtcgcctccc gtctcccgcc caagtaccgc accgcccgat
     1561 ccctggtgct gatcgtcgcc ctcaactcgg tgggggtgct tgcctacatc ggcaccgatc
     1621 aattgcgggt ggtggtcgtc gccggagtgt tctggggcct tctcatcggg atcatggccc
     1681 cgctgctccg caccctcatc cagctcaaca ctcccgagga tctgatgggt cggatcacgg
     1741 gtgtcaccca ggttcattcc gaggtcggcc atctgctccc cctcgccatc gcacctgtcc
     1801 tcgccgggat gtttggggtc cagcgaaccc tgatctacgc cggtcttgcc ctcgccgtct
     1861 tggccctctt cttcgttccc acggccaacc gcctcgaccg gacgcggagt gtcgaagtcc
     1921 caccgacggg gcttcccgat cccaacgacg agcctcgctc ggttgggcat tgaccagggg
     1981 cgggacggcc gcccctcgtt agagtctcgc cgccatggga ggagcgaatc gaaaggttgc
     2041 ccgggccgga atctcgatcg tggccgtcat cgtggtgttc gtcctggtgt gggagggcta
     2101 caagctgatc tggaccgccc tggggtggga gaacccggtc cgacccgacg accgcaccat
     2161 gccccacacc tgggacatct ttcttgccct cttccggccg gcacaacgga acggcccacc
     2221 tttcatcatc gtggagttga gggcggcatt gatgaccctg cgcgaggcct tcgtcggctt
     2281 cctcatcggt ggtgccgttg gctttgggct cggggtcctg ttcgtgagat cgaccgtgtc
     2341 ggagcgtgcc ttcatgccct acgtggtcgc ttcacagacc gtgccgataa tcgccatcgc
     2401 cccgatggtc gtggtctggg ggggccgcgc cgatcttccc caatggctga gcgtgtcgct
     2461 catcgccgcc tatctcacgt tcttcccggt ggccatcaac acgctgcgtg gattgcgctc
     2521 gccgcccccg acgtcaatgg agctgatgcg gtcctatgcg agcagccccg gcgaggtgtt
     2581 gtggaagctc caggtcccgg cggcgatgcc ctatatcttc actgccctca aggtctcggc
     2641 ggcggccagt gtcatcggag cgatcgtcgg tgagctcccc gccggtctac gtggtggctt
     2701 ggggcgcgac ctcctccagt tctcccaaca gttcaccgcg gctcccgaga agctgtttgc
     2761 cgcggtgatg atcgccgggc tggccggtat cgcgttcgtg ggactcatca ccgccttgga
     2821 gcatcgctgg attggagccc atcggaggct tgcttcgtga gcgcgcccgt ggtggatgcg
     2881 cagggcattg gcaaggtctt cggcagcggt gactcgcaaa cggaggcctt gcgcggcatc
     2941 gaccttcaga tcgcggacgg cgacttcgtt tcactgattg ggccttcggg ctgcggaaaa
     3001 tcgacgttgc tccggctgat cggggacctg gtcagcccca ccacaggaac cctggtcgtc
     3061 aatgggaagg agccaggtca ggctcgtctc gaccgcgact acgggatcgt cttccagtcg
     3121 gcgacgttgc tcgactggcg cagcgtccag aagaacgtcg agctaccact cgagatcatg
     3181 gatttccccg acaacgagcg gccgatccgt gcgctcgaga tgctcgacct ggtccagctg
     3241 ggggcatttg ccgaccacta cccctggcaa ctctcgggcg gaatgcagca gagggtggcg
     3301 atcgccaggg cgctcgcgtt ccggcccgcg ctactcctca tggatgagcc gttcggcgcc
     3361 ctcgacgaga tgacacgcga agccatgcaa caggaggtgc tccgggtatg gggggagacg
     3421 gggacgagtg tggtcttcgt cacccattcc atcccggaag cagtgtttct ctcgacgagg
     3481 gtggttgtga tgtcgccccg accggggcga atcagcgagg agatcatggt cgatctccct
     3541 ccggcccgtg accaggacac ccgcgaagac ccgagatact tcgagctcgt gatcgaagtg
     3601 agggaagcat tgcgccgtgg cgaatcgaag agcgggaaat cgtgaccgaa cgcgacgacc
     3661 tgatacagga gacgtcgacc accgatgtct tcgccgcatt cgaaggcgac cttcctgcgg
     3721 ttccccgacg atcgtccctg cgccgtctgt cccgccgcta tctgcccgcg gtcctcgctt
     3781 tcgtcgcggt gctcgtggta tgggaagggc tcaccaggct gttcggagtc gagtccttcg
     3841 tcctgtccaa accaagcgag atcctgaaca gcttcgcaga gacctccgac gtgatctggg
     3901 gggccgggat gagaaccctg ttcgaggctg tcggtgggtt tgtcatcggg accagcttgg
     3961 cggtcgccac gtcattggcg gccgcccatt gggttggctt ccggcaaggc gtgctacctc
     4021 tcgctatcgc gctcaacgca accccgatcg cggcgctggc accgatcttc aacaactggt
     4081 ttgggttgac caaccccttt tccaagatgg ccgtcgtggc ggtggtggtg tatttcccgg
     4141 tgatgatcaa cacgactcga ggattgctag aagtcgaccc ggccgaattg gagttgatgc
     4201 gctcgctcgc cgcgagcccg gcagaagtga cccggaggat ccgcatcccc ggtgcgttgc
     4261 cctatttctt tgcctcgctg aaggtcgcca gcgccctggc gctgatcgcg gccatcatcg
     4321 ccgagtactt cggcggcccc caggacgtgc tgggccaata catcgtcaac cgggcacaac
     4381 tcttccagtt ccccgacgcc tgggcggcga tcctggtggc ctcgatcctg ggcgtggtct
     4441 cgtatgtgct gatcctcgcc gccgaacggt ggctcatgcc ctggcacgtc tcgttccgta
     4501 gcaacgacta gcaaccgatt gcccggtgcg agcacgcact ccgccagata acgtgagccg
     4561 gcatcaggaa tggaggaatc cgatgaacac gagcaaatgg cggcgctacg tcctattgct
     4621 gtccgccctg gccatcgtcg tggccgcctg tggtgacggc ggtgagacgg gtggcggggg
     4681 gaccgggaca accgcaccgg ccgaggaatg caccgaaccc gaccaggtca ggctgcagct
     4741 gcagtggttc gcccagtcgc agttcgccgg ctactacgtc gcccgcgaca acggcttcta
     4801 cgaggcgcag tgcctcgaag tcgagatcat cgagggcgcg gtcgagatcg tgccacaaca
     4861 ggtgcttgcc accggtggtg cagagttcgg tctcgcctgg gtgcccaagg cgctggtttc
     4921 ccgtgagggc ggcgccgaca tcgtcaacat cgcccaggtg ttcgagcgtt cggggacgct
     4981 ccaggtctcc tgggccgacg ccgggatcga ggagccggct gactgggcag ggaagaaagt
     5041 gggtaactgg ggtttcggca acgagttcga gctcctcgcc gcgattgcca agtacgaggt
     5101 ccccgatgtc gagctggttg cacaggattt caccatgaac gccctgctca acaacgagat
     5161 cgatgccgcc gaggccatga tctacaacga gtacgcccag gtcctggagg cggtgaaccc
     5221 cgacacaggt gagctctaca ccccggatga cctgagtgtg atcgactaca acgagatcga
     5281 gacggccatg ctgcaggatg cggtttgggt caacgccgac tggatcgcag aagagggcaa
     5341 tgacgatgta gccacacggt tcctgacggc ctcgttccag ggctggatcc actgcctcga
     5401 caacttcgac gaatgcgtcg aggtcgtgct caacaacgga tcgacgctcg ggcagagtca
     5461 tcaggaatgg cagctcaacg agatcctcgg cctgatcttc cccgccacca acggcatcgg
     5521 ggtgatgaac gaggacttgt gggaccagac ggtggcgatc gccaccgagc agatcgccga
     5581 gctgcaggga gtcgagatcc ccgacgaggc ctatcggacc gacctcgccc aggcagcggt
     5641 ggatgctctg ggtgatgcgg atgtcacggg agaggccttc gaacggcgcg acatcgagct
     5701 ccaggagggt gggagctagc gagcgttctg cgttttcgtc gtaggtgcaa cgaagcggcg
     5761 ccaccctcag ggtggcgccg ctctccgcgc taggtttggg aaaaaggaga gagccgaatg
     5821 cccaacatcg tcagagccgc catcgtccag accgagtgga ccggcgacaa ggactcgatg
     5881 atcaagaaga acgcggacta cgcccgcgag gcggcgcagc agggagcgca ggtgatgtgc
     5941 ttccaggagc tgttctacgg cccctacttc tgccaggtac aggagaacga gcatttcgag
     6001 tacgccgagc cgatcccgga cggcccaacc acccagttga tgcaggacct tgccaaggag
     6061 acgggaatgg tgctcgtcgt cccgatcttc gagaagga
//
LOCUS       DASTXO010000536         2165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1506082, whole genome shotgun sequence.
ACCESSION   DASTXO010000536 DASTXO010000000
VERSION     DASTXO010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506082"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VFU96_11380"
     CDS             complement(<1..710)
                     /locus_tag="VFU96_11380"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEU5113909.1"
                     /translation="MRKLTIDQARRFALGAQGFTDPRPTGRVDVRHFRRVVDRIGLVQ
                     LDSVNVFSRTHYMPFFSRLGPYDRAALDAWLWGSGEMFEYWGHEASLIPVQDHYLWRW
                     RMNGKFDWARIEQMREEHPEYLDQVLEQVRDRGPLKAADLHDPGQRDGTSMWGWSKGK
                     VALEALFVGGRVTTAHRPNFTRLYDLSERVIGHQHLEMTGLEREEAQSVLLVKAARSM
                     GVATAADLADYPRIKIPEA"
     gene            772..2085
                     /gene="purB"
                     /locus_tag="VFU96_11385"
     CDS             772..2085
                     /gene="purB"
                     /locus_tag="VFU96_11385"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU5113910.1"
                     /translation="MIERYTLPEMARIWSEQRKLEVWKEVETLALEAWQALGKVPAGV
                     AAATQAATCPTPEQVAEREQVTNHDLAAFVDLLGEAAGPDAAGWVHYGLTSSDVLDTA
                     GGVLLSESADLLVGAAEALFETVIDRAMEHRDTYMVGRTHGIWAEPTTFGLKLAGWAF
                     ELARDHARLQEARAMVAVGKISGAVGTYAHVSPEVERHVCDALGLEPEPASTQVTARD
                     RHAQYLQTMALVGASIERMATEIRHLQRSEVAEVREAFGKGQKGSSAMPHKRNPILSE
                     RMTGMARLLRGYAQVGLENVALWHERDISHSSAERVVLPDSSIALHYMLVKFNALVRD
                     LVVDTNQMTANLESTRGLVFSQAVLLALIDNGMTRDQAYRIVQGHAMTAWDKGQALYE
                     LLAADPGVELTEETLDACFSLERVGEATRIVFDRLADLKLERFSP"
BASE COUNT          398 a          702 c          705 g          360 t
ORIGIN      
        1 gcctcgggga tcttgatccg tgggtagtcg gccagatcgg ccgcggtcgc cacacccatc
       61 gagcgggcag ccttgacgag tagcaccgat tgcgcctctt cccgctccag cccggtcatc
      121 tcgaggtgct ggtggccgat gactcgctcg ctcaggtcgt acagccgagt gaagttgggg
      181 cgatgggcgg tggtcacccg cccccccacg aacagggctt ccagagcgac ctttcccttc
      241 gaccaacccc acatggaggt cccatcgcgt tgaccggggt cgtggaggtc ggccgccttg
      301 agcggcccac gatcacgaac ctgctcgagc acctggtcca gatactcggg gtgctcctct
      361 ctcatctgtt cgatacgagc ccagtcgaac ttcccgttca tgcgccatct ccacaagtag
      421 tggtcctgga ccgggatcag agatgcctcg tgtccccagt actcgaacat ctcaccggag
      481 ccccacagcc aggcatcgag ggcggcgcgg tcatacgggc cgaggcggga gaagaagggc
      541 atgtagtggg tgcggctgaa gacattgacc gagtcgagct ggacaagacc tatccggtca
      601 acgactcgtc ggaaatgacg cacgtcgacc cggcccgtcg gtcgggggtc ggtgaagccc
      661 tgggcaccca gtgcaaaacg gcgcgcctgg tcgatggtga gcttgcgcat cgagcggcaa
      721 cctaacgccg atagcggaca gcgcatgtcc tagctctgcc atcatcccgg ggtgatcgag
      781 cgctacacgc tccccgagat ggcccggatc tggtccgagc agcgcaagct cgaggtgtgg
      841 aaggaagtgg agacgctcgc cctggaggcc tggcaggcgc tgggaaaggt gccggcaggc
      901 gtggcggcgg caacccaggc cgcgacgtgt cccacccccg aacaggtggc agagcgcgaa
      961 caggtgacca accacgacct cgccgccttc gtcgacctgc tcggagaggc agcaggccct
     1021 gacgcggccg gatgggtcca ctacgggctc acctcatccg atgtcctgga cactgcaggg
     1081 ggtgtcctcc tatctgaatc agccgacctt ctggtcggtg cggcggaggc actgttcgag
     1141 acggtcatcg accgcgccat ggagcaccgg gacacctaca tggtgggccg gacccacggg
     1201 atctgggccg agcccaccac attcgggctc aaactggccg gatgggcctt cgaactggcc
     1261 cgggatcacg cccgtctcca ggaggctcgc gcaatggtgg ctgtcggaaa gatctcgggt
     1321 gcggttggca cctacgcgca tgtctcgccc gaggtggagc gtcacgtctg tgacgccctc
     1381 ggcctggagc cagagccggc ctcgacccag gtcacggccc gagaccgcca cgcccagtac
     1441 ctgcagacga tggctctggt cggtgcctcg atcgaacgta tggcgaccga gatccgtcac
     1501 cttcagcgct ccgaagtggc agaggtcagg gaggcattcg gcaaagggca gaagggctca
     1561 tcggcgatgc cgcacaagcg gaacccgatc ctgtccgagc gcatgaccgg gatggccagg
     1621 ctccttcgag gttacgccca ggtgggtctg gagaatgtcg ccctgtggca cgagcgggac
     1681 atctctcatt catccgcgga gagggtggtt ctgcccgatt cgtcgatcgc gctccactac
     1741 atgctggtca agttcaatgc cctcgtgcgc gaccttgtcg tcgacacaaa ccagatgacc
     1801 gccaacctcg aatcgacacg tggcctcgtg ttcagccagg cggtcctgct cgcgctgatc
     1861 gacaacggca tgactcgtga ccaggcctat cggatcgtcc aaggtcacgc gatgacagcc
     1921 tgggacaagg gccaggcgct atacgagctg ctggcggccg atcccggcgt cgaactgacc
     1981 gaggagacac tcgacgcctg tttctccctg gaacgcgtgg gcgaagcgac acgaatcgtc
     2041 ttcgacagac tggccgacct gaagctggag cgattcagcc cctaggggtg atgtggacgg
     2101 tccccgtgaa cagggccact gtccgcccat cttcacgact gacatcggcc cggtaggtgc
     2161 cgaga
//
LOCUS       DASTXO010000537         2251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1616385, whole genome shotgun sequence.
ACCESSION   DASTXO010000537 DASTXO010000000
VERSION     DASTXO010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1616385"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="VFU96_11390"
     CDS             <1..942
                     /locus_tag="VFU96_11390"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEU5113911.1"
                     /translation="WKVTAFGQTTAGTHVAGVGSNWFGVGVHHSDNLIDWKPSDSPPA
                     WPEGSERKMEQIWTFHNEGDRLLAGVAQAGLFSSDDGGMSWAPIENLNEHRTREQWNP
                     GAGGLCAHRILSGSGSMWVGISAVGVFRSDDEGTTWVPKNDGIPAVDTADGGERPEVG
                     YCVHGLAHDQAEPSRMWRQDHQGVFRSTDGGDNWERIEEGLPAGFGFVMWRHPGTGRL
                     LTIPLESSENRVPVDGKLRVYASDDDGASWRVAGSGWSDAPQFTGVLRRAFDGDGDGG
                     FCFGTSGGKLWLTRDVGATWQELSASFPRIAAVSIVA"
     gene            939..1202
                     /locus_tag="VFU96_11395"
     CDS             939..1202
                     /locus_tag="VFU96_11395"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEU5113912.1"
                     /translation="MSVTVRIPRVLSQAVTTGLRHEAQGATLGAVLEDLFIREPGLEN
                     HLLDEDGKIRTHVLIFVDAARADLDTPVGPSSDVQVLQAVSGG"
     gene            complement(1181..1621)
                     /locus_tag="VFU96_11400"
     CDS             complement(1181..1621)
                     /locus_tag="VFU96_11400"
                     /inference="COORDINATES: protein motif:HMM:NF016211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEU5113913.1"
                     /translation="MTQSVDRSVAPRKPRSRLLRGIYFVLGIICLGFVYLSVLPGIPT
                     FDFVILAAFFFSMSSEKVHGWLVNHPVYGKIIKGYRSGGLTVRMKWAAAIAITASLSF
                     SAFVLIDNMVLRIILGAVGVFAIWFVFSRPTKRDQPPDQPPLTA"
     gene            1724..>2251
                     /locus_tag="VFU96_11405"
     CDS             1724..>2251
                     /locus_tag="VFU96_11405"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEU5113914.1"
                     /translation="MPIVTPHKYREMLAAARANGFALPAINVTSSTTIVAALEGLSQA
                     GSDGILQVSYGGGEFASGQSNKNMALGAIALADFAGVVGDQYPVNVALHTDHCPPDRV
                     DGFIRPILEESRHRREQGRGPVFQSHMLDASELPMEENLALARELLDLCTELDVILEL
                     EIGIVGGVEDATDNED"
BASE COUNT          407 a          708 c          753 g          383 t
ORIGIN      
        1 tggaaggtga cggcttttgg ccagaccact gcgggcactc acgtcgccgg cgtgggcagc
       61 aactggtttg gtgtcggcgt gcaccacagc gacaacctga tcgactggaa gcccagtgac
      121 tcccctccag cttggcccga aggctccgag cgcaagatgg agcagatctg gacatttcac
      181 aacgagggag accgcctgct cgccggcgtc gcccaggccg gtctcttctc atccgacgac
      241 ggggggatga gctgggcgcc gatcgaaaac ctcaacgagc accgcacgcg cgagcagtgg
      301 aaccccggag cgggtggcct ctgcgcccat cgcatcctgt ccgggagcgg gagcatgtgg
      361 gtcggcatct cggccgtagg tgtgttcagg tctgatgacg aaggcaccac ctgggtgccg
      421 aagaacgatg ggattcccgc cgtggacacg gccgacggtg gtgagcggcc ggaggtcggg
      481 tactgcgtcc acggcctcgc tcacgaccag gcggaaccga gccgaatgtg gcgccaggac
      541 caccagggtg tcttccgatc caccgacggt ggtgacaact gggagcggat cgaggagggc
      601 ctcccggccg ggttcgggtt cgtgatgtgg cggcatcccg gcaccggccg ccttctcacc
      661 atcccgctcg aatcgtccga gaacagggtc ccggttgacg gcaaactccg cgtctacgca
      721 tccgacgacg atggtgcctc gtggcgcgtc gccggcagtg ggtggtcgga cgccccacag
      781 ttcaccggtg tcctccggcg ggctttcgat ggtgatggcg atggtggttt ctgctttgga
      841 acctcggggg ggaagttgtg gctcactcgt gacgtcgggg cgacctggca ggagttgagc
      901 gcctcgttcc ccaggatcgc ggcggtctcc atcgtcgcat gagcgtcacc gtccggatcc
      961 cgcgggtcct gtctcaggcg gtgaccaccg ggctgcggca cgaggctcag ggtgcaacgc
     1021 tgggggcggt gctggaggac ctcttcatcc gggagccggg gctcgaaaac cacttgctgg
     1081 acgaggacgg caagatccgg acccatgtcc tgatcttcgt ggacgcggcc cgagccgatc
     1141 tggacacccc ggttggacct tcttcggatg tccaggtcct tcaggcggtc agtggaggct
     1201 gatcaggagg ctgatcccgc ttcgtgggtc ggctgaacac gaaccagatc gcgaacacgc
     1261 cgaccgctcc caggatgatc ctcagcacca tgttgtcgat gaggacgaat gccgagaatg
     1321 agaggctggc ggtgatggcg atggccgcgg cccatttcat cctcacggtg agcccaccgc
     1381 tgcgataacc cttgatgatc ttcccgtaga ccggatggtt gacgagccag ccatggacct
     1441 tctcggacga catcgagaag aagaatgcgg ccaggatcac gaagtcgaac gtgggaatgc
     1501 ccgggaggac gctcagatag acgaacccca ggcaaatgat gccgagcacg aaatagatcc
     1561 cgcgcaacag acggctgcgc ggtttccgcg gcgcgaccga ccggtcgaca ctctgggtca
     1621 cctcgtcaag ctattgcgga cggtttctga cggcgagtcg gcaaatgcgg cggcctactc
     1681 ccgtggagag gcagatccgc ctgccactac gctccaatga gccatgccca tcgtcacacc
     1741 acacaaatat cgcgaaatgc tcgccgcggc acgcgccaat gggtttgccc tgccggccat
     1801 caacgtcacc tcctcgacca cgatcgtggc cgccctcgag ggtctgagcc aggccggttc
     1861 ggatgggatc ctgcaggtga gctacggcgg aggcgagttc gcctccgggc agtcgaacaa
     1921 gaacatggcg ctgggcgcca tcgccctcgc cgatttcgcc ggcgtcgtcg gcgatcagta
     1981 tccggtcaat gtcgcgttgc acaccgacca ctgtcccccc gaccgcgtcg acggcttcat
     2041 tcgaccgatc ctcgaggaga gtcggcatcg tcgggagcag ggacggggcc ccgtatttca
     2101 gtcccacatg ctcgacgcct cggagctgcc gatggaagag aatctggccc tggccagaga
     2161 gctcctcgat ctgtgtaccg agctcgacgt catcctcgag ctggagatcg gcatcgtggg
     2221 gggcgtggag gacgccactg acaacgagga c
//
LOCUS       DASTXO010000538         3031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1026705, whole genome shotgun sequence.
ACCESSION   DASTXO010000538 DASTXO010000000
VERSION     DASTXO010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026705"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="VFU96_11410"
     CDS             <1..1112
                     /locus_tag="VFU96_11410"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEU5113915.1"
                     /translation="KIPGFGREVQTRLYAAIKFFLFTLFGSAFMLLGFLALYFQSGES
                     GERTFSIPQLTDLGASGAFTGRFAFLVFFAMFLGFAVKVPMWPLHTWLPDAHTAAPTV
                     GSVLLAAIMLKLGSYGFIRISLPILPEEAASWAPAIAILAVIGIIYGSLACLAQTDMK
                     RLIAFSSVGHMGFVMLGIATMTDVGINASIIGMVAHGLITGLLFFLAGSMSHRYHTRE
                     MSRLGGNMKLMPVMGGILAFTAMASLGLPGLAGFWGEFMSLIGAYNPLEGLSLGVFRT
                     AMVLGAIGTVLTAAYMLYLLQQVNLGEPGEEWQGHEFRDVDRFEMTAWVPLIVLIVVI
                     GFYPKVIFNATTDSVVSLVDMIFNSETVASLGSGG"
     gene            1116..2600
                     /locus_tag="VFU96_11415"
     CDS             1116..2600
                     /locus_tag="VFU96_11415"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEU5113916.1"
                     /translation="MQFEIDYLALAPELVVVATMLGVFALDLILPRPKKFWVATLAVA
                     GIAVAAVPLVMLAADGTTRSMFDGSYVVDEFAVILKGLFIVSAYIVLLMSHHYIESER
                     FYQGEYYFLLLASVLGSLVMASSRDLIILFIGLELTTGPLYLLAGWRKGDAKSNEASM
                     KYFILGVLSTAIMLYGMSLLFGLTGEVTFAGLAEATAGLADEPIMVVALFGLIVGFGF
                     KISAVPFHFWAPDTYEGAPTPVTAYLSVNSKAAGFVAFLLIMYQALPGISEVWAPALW
                     ILAVLSMTVGNLAALRQTNIVRLLAYSSIAQAGFMLVPFAAAAVAGADLAEAFSATLV
                     YLVIYAVMNLGAFAVVIAGARKTRSGEIRAWSGLSQVDVRLAVLVAVFFFSLAGIPPL
                     AGWFAKFAMFRSVIIAGGTATVTLAAIAAVNSVIALYYYARVVKAVWLDDPVGEFAPD
                     VEAAPAGSLRLALLVSLVITVAIGFFPAIATFVGNASRVIATGG"
     gene            complement(2631..>3031)
                     /locus_tag="VFU96_11420"
     CDS             complement(2631..>3031)
                     /locus_tag="VFU96_11420"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU5113917.1"
                     /translation="SDEPAATWRSNTYGLTAGGMPDGGMTTTAGDLDRFIDALREGRI
                     LSEETLSSMLTVHGTDDEESYGYGMELVTDNDSVIIYGHSGLDPGVTAVVSHYVGDGI
                     TVIVLCNQDQGSWPVAQRIAADLGLHDPRD"
BASE COUNT          429 a         1019 c          912 g          671 t
ORIGIN      
        1 ggaagatccc cgggttcgga cgcgaggtgc agacccgtct ctatgccgcc atcaagtttt
       61 tcctcttcac cctgttcggg tctgccttca tgctgttggg attcctcgcc ctctacttcc
      121 agtcgggtga atcgggggag cgcaccttct ccatcccgca gttgaccgac ctgggcgcct
      181 ccggagcgtt caccgggagg ttcgccttcc tcgtcttctt cgccatgttc ctcggcttcg
      241 ctgtcaaggt cccgatgtgg ccgctccata cctggcttcc tgacgcccac actgccgccc
      301 ccacggtggg gtcggtgctc ctcgccgcga tcatgctgaa gctcgggtcc tatggcttca
      361 tcaggatctc cctccccatc cttcccgagg aggccgccag ctgggccccg gccatagcca
      421 tcctggccgt gatcggaatc atctacggct cgttggcctg tctggctcag accgacatga
      481 aacgtctgat cgccttctct tcggtcggtc acatgggttt cgtcatgctc ggcatcgcca
      541 ccatgaccga cgtggggatc aatgcctcga tcatcgggat ggtcgctcac ggcttgatca
      601 ccgggctcct cttcttcctc gccgggtcga tgtcgcaccg ctaccacacc agggagatgt
      661 cacgtctcgg gggcaacatg aagctgatgc cggtgatggg cggcatcctg gccttcactg
      721 ccatggcctc gctcgggttg cccgggcttg ccgggttctg gggggagttc atgtccctca
      781 tcggggccta caacccgctg gaggggctct ccctgggcgt gttccgcacc gcgatggtgc
      841 ttggcgccat cggcacggtg ctgaccgccg cctacatgct ctatctcctt cagcaggtga
      901 acctcggcga gcccggtgag gaatggcagg gacacgagtt ccgcgacgtc gatcgcttcg
      961 agatgaccgc ctgggtcccg ctcatcgttc tcatcgtggt gatcgggttc tatcccaagg
     1021 tcatcttcaa cgccaccacc gacagcgtgg tctcgctggt cgacatgatc ttcaattctg
     1081 agaccgtcgc ctcattgggg tctggaggct gacctttgca gttcgagatc gactatctgg
     1141 cgcttgcccc cgagctggtg gtggtcgcca ccatgctcgg ggtcttcgcc ctcgatctca
     1201 tcctgccccg gcccaaaaag ttctgggtcg cgaccctggc cgtggccggc atcgccgtcg
     1261 ccgcggtgcc cctggtgatg cttgccgcgg acgggacgac gagatcgatg ttcgacggct
     1321 catacgtggt cgacgagttc gcggtgatcc tcaagggtct gttcatcgtc tccgcctaca
     1381 tcgtcctcct catgtcacac cactacatcg aatccgagcg cttctaccag ggcgagtact
     1441 acttcctcct cctcgcctcg gtgctcggct cgctggtgat ggcttcttcc cgtgacctga
     1501 tcattctctt catcgggctc gagctcacca ccgggccgct ctatctcctg gccgggtgga
     1561 gaaaggggga tgccaagtcg aacgaggcct cgatgaagta cttcatcctc ggagtcctct
     1621 ccactgcgat catgctctac gggatgtcgc tgctgttcgg actgaccggt gaggtcacct
     1681 ttgccggcct ggccgaagcc acggcaggcc ttgccgatga gccgatcatg gtggtggccc
     1741 tgttcgggct catcgtcggc ttcgggttca agatctcagc cgtcccgttc cacttctggg
     1801 caccggacac ctacgaaggg gcccccacgc cggttaccgc ctatttgtcg gtcaattcca
     1861 aggccgccgg ctttgtcgcc ttcctcttga tcatgtacca ggccctgccc ggcatctccg
     1921 aggtctgggc cccggcactg tggatcctgg ccgtgttgtc gatgacagtg ggcaacctcg
     1981 ccgcacttcg gcagaccaac atcgtccggc ttctcgccta ctcctcgatt gcccaggccg
     2041 ggttcatgct cgtgcccttc gcggcggcgg ccgtcgcggg tgcagacctc gccgaggcct
     2101 tctcggcaac gctggtctat ctcgtcatct acgccgtcat gaacctcggg gcattcgcag
     2161 tcgtgatagc gggggccagg aagaccagat ccggcgagat ccgagcctgg tcgggcctct
     2221 cccaggtcga tgtcaggctg gcggtgctgg tggcagtctt cttcttcagt ctcgccggca
     2281 tcccgccctt ggccgggtgg ttcgccaagt tcgccatgtt ccgctcggtc atcatcgccg
     2341 gtggcacggc cacggtcacc ctcgccgcca tcgccgcggt caactcggtg atcgccctgt
     2401 actactacgc ccgggtcgtg aaagccgttt ggctcgacga cccggtgggt gagttcgcac
     2461 cagacgtcga ggctgcacct gccggatcgc tgcgtctggc attgctggtc tccttggtga
     2521 tcacggttgc catcgggttc ttcccggcca tcgccacctt cgtcggcaat gccagccggg
     2581 tcatcgccac cggaggctga agtcggccta cgcgattccg atgggaccgg tcaatcccga
     2641 gggtcgtgga gaccgagatc tgcggcgatt ctctgcgcaa cgggccagga tccctgatcc
     2701 tggttgcaca ggacgatcac ggtgatcccg tcgccgacat agtgcgagac gaccgcggtg
     2761 acgcctgggt cgaggccgct gtgcccgtag atgatcaccg agtcgttgtc ggtaaccagc
     2821 tccatgccat agccgtagga ctcctcgtcg tcggtgccgt ggacggtgag catcgagctc
     2881 agggtctcct ccgaaaggat ccgcccttcc cgaagggcgt cgatgaaacg gtccaggtct
     2941 ccggcggtgg tggtcatccc accgtcgggc atgccgccgg ccgtcaagcc gtaggtgttg
     3001 gagcgccatg ttgccgccgg ctcgtcgctg a
//
LOCUS       DASTXO010000539         2089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_769619, whole genome shotgun sequence.
ACCESSION   DASTXO010000539 DASTXO010000000
VERSION     DASTXO010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769619"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFU96_11425"
     CDS             complement(<1..306)
                     /locus_tag="VFU96_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEU5113918.1"
                     /translation="MGSALSEHTGLTPVKIVVAGGFGVGKTTFVGAISEIEPLRSEAM
                     ITDASAGIDELYPDGAKTETTVAMDFGRISLKHRLVLYLFGTPGQDRFHFMWDELARG
                     "
     gene            complement(287..667)
                     /locus_tag="VFU96_11430"
     CDS             complement(287..667)
                     /locus_tag="VFU96_11430"
                     /inference="COORDINATES: protein motif:HMM:NF017170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF742 domain-containing protein"
                     /protein_id="HEU5113919.1"
                     /translation="MTTQPDIGVSGRIVRPYTMTGGRSGVGLPVIALEALVAVTPAGM
                     RMRGRFRWEAAEIIAQSRREIAIVELAAILEVPVGVVRVLAIDLRDQGAITITEPPVE
                     TISESADGDYADLLHKVLDGIRAL"
     gene            complement(664..1089)
                     /locus_tag="VFU96_11435"
     CDS             complement(664..1089)
                     /locus_tag="VFU96_11435"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEU5113920.1"
                     /translation="MTDAATNLNWLLERLCDTVPAIKQAVVVSSDGLAMAKSQDVDRE
                     TAERLAAVSSGMIGLAYGSAGRFGAGAVSNIIVEMQQGWLFITGIRHGSLLCCLTSKE
                     IDLGAVAFEMSIFVQRVGDSLTPDVREELKARLVEPEPA"
     gene            complement(1082..>2089)
                     /locus_tag="VFU96_11440"
     CDS             complement(1082..>2089)
                     /locus_tag="VFU96_11440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5113921.1"
                     /translation="GDLSRSFENMHRALIDTSARQMESLRVGVSSILVTLARRNSSLV
                     DRQLALLDELESREEDPATLGGYYQVDHLAARMRRNAESLLVLAGSESPRLWAKATDI
                     SDVVRAALSEVDEYQRIELLALEPARLSAGSVTDVAHLLAELFENSVQFSPPTEPVRV
                     TGLFDVDGYELSISDRGVGMSDPRLAELNRILERPPALGLSVEPTMGIYVVAKLAHRH
                     GLSVQLIPSVPGITARLTIPRERLETTKAVEPEHEIIDLTQPEMVVTTPSEPIPDSTP
                     TIAEPEVADLPVRIPGRAFREEEPAPSIAAGDGGGTLRSALIDYNRGRSEAEAMEDSN
                     D"
BASE COUNT          375 a          673 c          677 g          364 t
ORIGIN      
        1 gcctcgagcc aactcatccc acatgaaatg gaagcggtcc tgtcccggag tgccgaacaa
       61 atagaggacc aagcgatgct tgaggctgat ccggccgaaa tccatcgcca ccgtggtctc
      121 ggtcttggcg ccgtcgggat acaactcgtc gatcccagcg ctcgcatcgg tgatcatcgc
      181 ctcactccgc aaaggctcga tctcggatat ggcccccacg aacgtggtct tgccaacgcc
      241 gaagccgccg gcgacgacga tcttgaccgg ggtcaacccg gtgtgttcag agagcgcgga
      301 tcccatcgag caccttgtgt aggaggtcgg cgtagtcgcc gtcggccgat tcggagatcg
      361 tctcgaccgg cggctcggtg atcgttatcg ctccctggtc tcgaagatcg atggcgagaa
      421 ctctgaccac gccgacggga acctcgagaa tggccgccag ttcgacgatg gcgatctccc
      481 tgcggctttg ggcgatgatc tcggccgctt cccagcggaa ccggccccgc atccgcatcc
      541 cggcgggcgt cactgcaacc agggcttcga gagcgataac ggggagtccc accccgcttc
      601 gaccaccggt catggtgtag gggcggacga ttcgaccgct gacaccgatg tcgggctgag
      661 tggtcatgcc ggctcgggtt cgaccagccg cgccttgagc tcctcccgga cgtcgggggt
      721 caggctgtca cccactcgtt gcacgaagat cgacatctcg aacgccaccg cccccagatc
      781 gatctccttg ctggtgaggc agcagagaag cgagccgtgg cggatccccg tgatgaagag
      841 ccagccctgt tgcatctcga cgatgatgtt cgacacggca ccggcgccga atcggccggc
      901 ggacccatag gccagcccga tcatgcccga agacaccgcg gccaaacgct ccgcggtctc
      961 ccgatcgacg tcctgagact tggccatggc cagcccgtcg gacgagacca cgaccgcctg
     1021 tttgatggcg ggcacggtgt cgcagaggcg ctccagaagc cagttgaggt tcgtggcagc
     1081 gtcagtcatt gctgtcctcc attgcttccg cctcggagcg accccggttg tagtcgatta
     1141 gcgccgatct gagggtgccg ccgccatcgc cggccgcgat gctcggtgcg ggctcctcct
     1201 cgcggaaggc acggcccgga atgcgcaccg gaagatctgc gacttccggc tcggcgatgg
     1261 tcggcgtcga gtccgggatt ggctccgagg gcgtggtcac gaccatctcg ggctgggtga
     1321 gatcgatgat ctcgtgttcc ggctcgacag ccttcgttgt ctccaatcgt tcgcggggaa
     1381 tggtcaatcg ggcggtgatc ccgggaacgc tcggaatcag ctgcaccgag aggccgtgtc
     1441 gatgtgccag cttggccacg acgtatatcc ccattgtcgg ctcaacagag agacccaggg
     1501 cgggtggccg ctccaggatc ctgttcaact cggcaagtct cgggtcggac atcccgacgc
     1561 cgcggtcaga gatcgacaac tcgtagccat cgacgtcgaa gaggccggtc acccggacgg
     1621 gctcggtcgg cggcgagaac tgaaccgagt tctcgaagag ctcggcgaga aggtgggcga
     1681 catcggtgac ggaaccagcc gatagccggg ccggctccag ggcgagcaat tcgatccgct
     1741 ggtattcgtc gacctcactg agggcggccc ggaccacatc ggagatatcg gtggccttgg
     1801 cccagagacg gggcgattcc gacccggcca gcaccagaag gctttccgca ttcctacgca
     1861 tccgcgccgc cagatgatcg acctggtagt agcccccaag ggtggcggga tcctcttccc
     1921 tggactcgag ctcgtccagc agggccagct gtcggtcgac aagagagctg ttgcgacggg
     1981 ccagagtcac gaggatgctg gaaaccccga cccgcagtga ttccatctgg cgggccgatg
     2041 tgtcgatcag ggcccggtgc atgttctcga atgaccggga gaggtctcc
//
LOCUS       DASTXO010000540         5785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_696244, whole genome shotgun sequence.
ACCESSION   DASTXO010000540 DASTXO010000000
VERSION     DASTXO010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5785
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696244"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VFU96_11445"
     CDS             complement(<1..501)
                     /locus_tag="VFU96_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU5113922.1"
                     /translation="MGPESSHDDSASYPQPVISALFDESAWDLVPGFDFTDVTYHKAI
                     GHGVVRIAFDRPEVRNAFRPHTVDELYAALDHARQTTNIGCVLLTGNGPSPKDGGWAF
                     CSGGDQRIRGRDGYKYAAEDDDDTMDSARLGRLHILEVQRLIRFMPKIVIAVVPGWAV
                     GGGHSLH"
     gene            500..1693
                     /locus_tag="VFU96_11450"
     CDS             500..1693
                     /locus_tag="VFU96_11450"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU5113923.1"
                     /translation="MLDWLRRHDPGRLFIETPDETLTFGAAADAVRERPVRGAEIVRP
                     ALEVGSVVDLIALMAKGSALITGETADIASIDPAGAASVVFTSGSSGGRKGVRLDLDN
                     WEAAAKASMEHLDHGTDDVWLLALPLHHVAGLSIIVRSAFSGGAVRMLPDFDPATYAA
                     HIRRGVTVASMVPTMVARLLAVDPGPFHGLRAVLVGGGPIPDRLLEQAHAAGLPVLPT
                     YGLTETAGQVATLRPGEPLENKAHPLPGVELRIEPGGRIAVRGPMVSPGYLGEPDRGA
                     EEWLVTGDLGTIDDDGALRVTGRADTMIVSGGENIDPADVEAAVVRVAGVEGALVAGL
                     ASDLWGMEMVCLYVGEAEPSRIEAELKDTLPGFMIPKRWLQVPELPLTPLGKPDRAQA
                     VRRFT"
     gene            complement(1686..2336)
                     /locus_tag="VFU96_11455"
     CDS             complement(1686..2336)
                     /locus_tag="VFU96_11455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEU5113924.1"
                     /translation="MAEMTPETWDYINSYSLEVFGAQDAHLAGLMDEATAAGLPPIAV
                     GPDVGRLLMILTSMTKGRLAVEVGTLAGYSGIWITRGLAPAGRLITIEREPLHADFAQ
                     RQFERAGVADRVELRRGAGIDVLAELSQSLEPGSVDVLFIDAHKPEYIGYFDTARALL
                     AIGGLVIADNVYGIGTGWIDHGYGTDEFNRHIAADPAFEAAAFPFREGVLIARRVR"
     gene            complement(2417..3355)
                     /locus_tag="VFU96_11460"
     CDS             complement(2417..3355)
                     /locus_tag="VFU96_11460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5113925.1"
                     /translation="MNRENTTLVDETGRLTSDSLSQYLAGIGGRPLLTAEDEVRLAQA
                     MEAGVEARHALDQDVDDPAERVRLEHVCREGERARQDFIQANLRLVVSNARLYSGAKV
                     DMLDLIQEGNLGLIRAVEKFDWRKGFKFSTYATWWIRQALQRARADQSDAIRIPSALF
                     DILPVVRGAAEELRTKLDRAPTSEEIAEITGISLADVDKALSVATTVALESPIGEDGA
                     LLGDFIADTDAIDPSTEVEQRIVEERLRQSLAHLSDLHRQVLELRFGFGDGVPAAFST
                     VANITGLPEADVRELVAEALVLLAEDLRAVEDMVAA"
     gene            complement(3430..3837)
                     /gene="arfB"
                     /locus_tag="VFU96_11465"
     CDS             complement(3430..3837)
                     /gene="arfB"
                     /locus_tag="VFU96_11465"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011643181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEU5113926.1"
                     /translation="MTSRGLAVGSWLIPEAEIEERFDTSGGPGGQHANKTETAVTLRL
                     DIAASSLPDEIKALLQRRLGEVVEVTASDTRSQWRNRDIARERLSERIERAMIQPKQR
                     KKTKPSQQSKERRLTEKRSRSQIKKGRRPPEAE"
     gene            complement(3834..5156)
                     /locus_tag="VFU96_11470"
     CDS             complement(3834..5156)
                     /locus_tag="VFU96_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEU5113927.1"
                     /translation="MSESQQPAPATGKLGILTPGMGAVATTFVAGVLAARKGYAHPFG
                     SLSQMSHIRLGKRTDGRNPLVKDFVPLAGLDDLEFGGWDPYSDDAYTSAMKAGVLDPR
                     DIEGLGDELRAVKPYPAVFSNRWVRNLDGVDQVKEEATHMDLAEGLMEDIDRFRGEKG
                     VDRLVMVWCGSTEAYEEPSEVHADLQTFEQGLKDNDPAICPSQIYAYAALKMKVPFAN
                     GAPNLTVDIPAMVELAALEGVPISGKDFKTGQTLMKTMVAPGLKARMLGVKGWFSTNI
                     LGNRDGEVLDDPDSFRSKEVSKLGVLEQILQPEVYPELYGDLYHKVRINYYPPKGDDK
                     EGWDNIDIFGWLGYPMQIKIDFLCKDSILAAPIVLDLALFMDLAARAGRTGVQEWLSF
                     YFKSPQAAPGLYAENDLFIQLTKLKNNLRHMMGEDLITHLGTEYYEEG"
     gene            5228..5683
                     /locus_tag="VFU96_11475"
     CDS             5228..5683
                     /locus_tag="VFU96_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113928.1"
                     /translation="MDRAPDSIGPTVDVALLADAVQAVRGKLFVLGGGWDTLWVRSFP
                     ARHPSMAIGLRLRVPTSWRAEVLDLSVELQDADGAPLLSKPLSHQVRLPPQPQSPSPT
                     DFGVVRSFTFNNLLFGSEGSYSFVISVDGDPVSRLRFSVRARPAPSAAG"
BASE COUNT          965 a         1827 c         1888 g         1105 t
ORIGIN      
        1 gtgcagtgag tgaccgcccc cgactgccca tcccggcacg acggcaatga cgatcttggg
       61 catgaacctg atcagacgct gaacctcgag gatgtggagg cggccgagtc gggcgctgtc
      121 catcgtgtca tcgtcatcct cggcggcgta cttgtagccg tcacggcccc ggatcctctg
      181 gtctccgccc gagcagaaag cccaccctcc gtcctttggg gatggcccgt tcccggtgag
      241 aaggacacag ccgatgttcg tggtctgacg ggcgtggtcg agggccgcgt acaactcgtc
      301 gacggtgtgt ggccgaaacg cgttacgaac ctcgggccgg tcgaaggcga tccgaaccac
      361 gccgtgcccg atcgccttgt gataagtcac gtcggtgaag tcgaaccccg gcacaagatc
      421 ccatgccgat tcgtcgaaga gcgcagagat caccggttga ggataagagg cagaatcgtc
      481 gtgtgaagat tccgggccca tgctcgactg gctgagacga cacgacccgg gccggctgtt
      541 catcgagaca ccggacgaga cgctgacctt tggggctgcc gcagacgccg tgcgagagcg
      601 cccggttcga ggagcggaga tagtgcggcc ggcgctcgag gtcggatcgg tcgtcgacct
      661 gatcgcgctg atggccaagg gctcggcctt gatcacgggc gagaccgctg acatcgcgtc
      721 gatcgatccg gccggcgcgg cctcggtggt cttcacctcg gggagctcgg gtgggcgaaa
      781 aggcgttcgt ctcgatctcg acaattggga ggcggccgcc aaggcctcga tggagcatct
      841 ggatcacggg accgatgacg tctggctgct cgccctgccg cttcatcacg tggccgggct
      901 gtcgatcatc gtcaggtcgg ctttctcggg tggggcggta cgcatgcttc ccgacttcga
      961 tcccgccact tacgccgccc atatccgacg aggagtgacg gtggcttcga tggtcccaac
     1021 catggtcgcg cgcttgttgg cggtcgatcc tggtccattt cacgggttga gggcagtgct
     1081 ggttggaggt gggccgatcc ccgatcgtct cctggagcag gcacacgctg caggtctgcc
     1141 cgtcctcccc acctacggcc tgaccgagac cgcgggtcag gttgccactt tgaggccagg
     1201 cgagcctctc gagaacaagg cgcatccatt gcccggcgtg gagctgcgca tagagcctgg
     1261 cggccggatc gcagtgcgtg gcccgatggt cagccccggc tacctcggag agccagatcg
     1321 cggtgcggaa gagtggctgg tgacgggcga cctgggcacg atcgacgacg acggcgccct
     1381 tcgggtgacc gggcgcgccg acacgatgat cgtcagcggt ggggagaaca tcgacccggc
     1441 cgatgtcgag gcggccgtgg tccgcgtcgc gggcgtggaa ggtgccctgg tggctggcct
     1501 tgccagcgac ctttggggga tggagatggt atgcctctat gtcggcgagg cggagccgag
     1561 ccggatcgag gcggagctga aagacacgct gccggggttc atgatcccca agcgttggct
     1621 ccaggtcccc gaactgcccc tcaccccgct gggaaaaccc gatcgggccc aggctgtgcg
     1681 ccgattcacc tgacccgtcg agcgatcagg acgccttctc ggaatgggaa cgccgccgcc
     1741 tcgaaggcgg ggtctgcagc gatatggcga ttgaactcat cggtgccgta tccgtggtcg
     1801 atccacccgg tcccaatgcc atagacgttg tcggcgatga ccagcccgcc gatggcgaga
     1861 agggcacggg cggtatcgaa gtagccgatg tactccggct tgtgggcgtc gatgaacagg
     1921 acgtccacgc ttccgggctc gagcgactgg ctcagctcgg caagcacgtc gatacccgcc
     1981 ccgcgtcgca gttcgacccg atcggcgact ccggcgcgct cgaattgtcg ttgagcgaaa
     2041 tcggcatgaa gtggctcgcg ctcgatcgtg atcagccggc cggcgggagc cagcccacgg
     2101 gtgatccaga tgccggagta tcccgccaac gtcccgacct cgacggccag tcggcccttg
     2161 gtcatcgacg tgagaatcat cagcaatcgg ccgacgtcag gccctacggc gatgggaggg
     2221 agaccggcgg cggtcgcctc atccatcaat ccggccagat gggcatcctg ggctccgaac
     2281 acctccagcg agtagctgtt gatgtaatcc caggtctcgg gggtcatctc tgccatgacc
     2341 gagacgttag gtcaggacga ggacggtcga atcgactcgc atgcctcgcc gctcggaatc
     2401 acactggtct gacgcttcag gcagcgacca tgtcctcgac ggcgcggaga tcttcggcga
     2461 gcaggaccag cgcctcggcc acgagctcgc ggacatcggc ctcgggaaga ccggtgatgt
     2521 tcgcgaccgt cgagaacgct gcggggacac cgtcgccaaa accgaacctg agctcgagca
     2581 cctgacgatg cagatcggag agatgagcca ggctctgacg aagtctctcc tccacgattc
     2641 gctgctcgac ttcggtcgac gggtcgattg cgtctgtgtc ggcgatgaag tcgccgagca
     2701 gagccccgtc ctcaccgatc ggggattcga gggccacagt ggtcgcaacc gacagcgcct
     2761 tgtcgacgtc ggcaagagag atccccgtta tttcggcgat ctcctcactg gtcggtgcac
     2821 ggtcgagctt ggtgcgcagc tcctccgcgg cgcctcggac gacagggagg atgtcgaaaa
     2881 gggccgaagg gatgcggatc gcgtcgctct gatcggccct ggcgcgttgc agtgcctgac
     2941 ggatccacca ggtcgcgtag gttgagaact tgaacccctt cctccagtcg aacttctcga
     3001 ccgcccgaat caggcccagg tttccttcct ggatcaggtc gagcatgtcc accttggccc
     3061 cggagtagag acgagcgttg gagacgacga gacggagatt ggcctggatg aagtcctgac
     3121 gtgctcgctc accctcccgg cagacatgtt ccagccggac ccgctccgcc ggatcgtcga
     3181 cgtcttggtc gagggcatgg cgcgcctcga cacccgcctc catcgcctgg gcgaggcgaa
     3241 cctcgtcctc ggccgtgagc aggggtcgac ccccgatccc cgccagatac tggctcaggg
     3301 agtcactggt gagacgtccg gtctcgtcga ccagcgtcgt gttttctctg ttcacacgat
     3361 ctctctgatt ttgcacaacc agtcgaacgt cagttcgcag tcagacattc cggcgctttc
     3421 gctcgagggt cattcggctt cgggaggccg gcgacccttc ttgatctgcg acctcgaccg
     3481 cttctccgtg agtcggcgtt ccttggactg ttgtgatggt ttggtcttct ttctctgctt
     3541 gggctggatc attgcccgtt cgatccgctc gctgagacgc tcccgcgcta tatcgcggtt
     3601 ccgccattgc gatcgggtgt cagaggcggt cacttccacc acctcaccga gccggcgttg
     3661 caggagggcc ttgatctcgt ccggcaacga cgaggcggcg atgtcgagac ggagggtgac
     3721 cgctgtttcg gtcttgttgg cgtgttgacc gcctggccca cccgaagtgt cgaaacgctc
     3781 ctcgatctcg gcctcgggta tgagccagga gccgaccgcg agcccgcgac tggtcacccc
     3841 tcttcgtagt actcggtgcc gagatgtgtg atcagatcct cgcccatcat gtgcctcagg
     3901 ttgttcttga gcttggtgag ctggatgaag aggtcgttct cggcgtagag gccaggggcg
     3961 gcctgtgggc tcttgaagta gaaggagagc cattcctgga ccccggtccg accggcgcgt
     4021 gcggccagat ccatgaacaa cgccaggtcg aggactatcg gtgccgccag gatcgagtct
     4081 ttgcacagga aatcgatctt tatctgcatc gggtaaccga gccacccaaa gatgtcgatg
     4141 ttgtcccatc cctctttgtc gtcacccttg ggcgggtaat agttgattcg caccttgtgg
     4201 tagaggtcgc cgtacagctc cggatagacc tctggttgga ggatctgctc caggacgccg
     4261 agtttggaga cctccttcga ccggaagctg tcggggtcgt cgagcacctc accgtctcgg
     4321 ttgccgagga tgttggtgga gaaccatccc ttgaccccga gcatgcgggc cttgaggccg
     4381 ggggccacca tggtcttcat caaggtctgg ccggtcttga agtctttgcc cgagatggga
     4441 acgccttcga gggcggccag ctcgaccatt gcggggatgt ccacagtgag gttgggcgca
     4501 ccgttggcga acgggacctt catcttcagt gcggcgtagg cgtagatctg cgaagggcaa
     4561 atggcaggat cgttgtcctt gaggccctgc tcgaatgtct gcagatcggc atggacctcg
     4621 gacggctcct cataggcttc ggtcgacccg caccacacca tcaccaggcg atccactcct
     4681 ttctctccgc ggaaccggtc gatgtcctcc ataaggcctt cggcgaggtc catgtgagtg
     4741 gcttcttcct tcacctggtc gaccccgtcg aggttcctca cccagcggtt tgagaacacg
     4801 gccgggtacg gcttgacggc ccgcaactca tccccgaggc cttcgatgtc ccgcgggtcg
     4861 agaacacccg ctttcatcgc cgaggtgtag gcgtcgtcgg agtaggggtc ccaaccgccg
     4921 aactcgaggt cgtcgagccc ggcgagaggc acgaagtcct tgacgagggg attccgccca
     4981 tctgttctct tgccgagacg tatgtgcgac atctggctca gtgagccaaa ggggtgggca
     5041 tagcccttcc gggcggcgag gaccccggcg acgaatgtgg tggcgacggc ccccatcccg
     5101 ggggtgagta tcccgagctt cccggtggcg ggggccggct gctgactttc ggacatgaaa
     5161 cctcctgccc gaaacgatag gggagagggg tgccggaacc gaaccccgcc cgcaataagg
     5221 ttcccccatg gatcgcgccc cggattccat cggccccacc gtcgatgtgg ctcttctcgc
     5281 cgacgcagtc caagcggtgc gggggaagct cttcgtcctc ggtggaggct gggacacgct
     5341 ctgggtcagg tcattccctg cccgtcaccc gagcatggcc atcggcctcc ggctccgggt
     5401 cccgacctcg tggagagccg aagtcctcga tctgtcggtc gagttgcagg acgcggacgg
     5461 ggcgccgttg ctctccaagc cgctctctca tcaggtgagg ctgccgccac agccacagtc
     5521 accgtcgccc accgatttcg gtgtcgttcg gtcattcacg ttcaacaacc tcctcttcgg
     5581 gagcgaaggc tcctactcgt tcgtgatctc ggtcgacggt gatcccgtct cacggctccg
     5641 cttcagtgtt cgggccagac ccgccccgtc ggccgcgggc tgagcccgtg atcccggtag
     5701 tgggtgaccg cacgcgcgtt gcgcgcgcat caaccactct tagcgcggag ttggtatagc
     5761 aacggggccg ctcatagggt tcccc
//
LOCUS       DASTXO010000541         4136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_327915, whole genome shotgun sequence.
ACCESSION   DASTXO010000541 DASTXO010000000
VERSION     DASTXO010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327915"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VFU96_11480"
     CDS             <1..385
                     /locus_tag="VFU96_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEU5113929.1"
                     /translation="RFGYVYEINMLRCIFCGLCVEACPTEAITMTSLFEISTTNREDA
                     IYTKETLLMDRRGNVPHMFPDDELADLDELDTADGWLRATAPSGVAAYEGVKMWEGTP
                     GPADWSPEPSQEERDRRTTDGEENG"
     gene            378..902
                     /locus_tag="VFU96_11485"
     CDS             378..902
                     /locus_tag="VFU96_11485"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEU5113930.1"
                     /translation="MVETVIFVIAALAALGGAVTVVLARNPIYSAVGLLGTLFSVAIL
                     YVVQLAHLVAAVQVIVYAGAVMTLFLFVIMLIGVDKEETRVEPLPRQRLWVGGLAGVV
                     IVFAAGVALFGDFVWVPAAAATVDQEATNGTIQAVAEPLFTDWVLAFEATALLLTIAA
                     AGAVVLAQFKRKRA"
     gene            902..1204
                     /gene="nuoK"
                     /locus_tag="VFU96_11490"
     CDS             902..1204
                     /gene="nuoK"
                     /locus_tag="VFU96_11490"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEU5113931.1"
                     /translation="MVPVGWYMALAAALFLIGALGMLLRRNALVMFMCIELMLNAVNL
                     TLISAGRAMNDLNGQVAVFFILVVAAAEVVVGLAIIVAIFRRRATASVDELSELRG"
     gene            1201..3084
                     /gene="nuoL"
                     /locus_tag="VFU96_11495"
     CDS             1201..3084
                     /gene="nuoL"
                     /locus_tag="VFU96_11495"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEU5113932.1"
                     /translation="MTDYVWLVIALPLAGALFLHFFGRYVKEPLSGWLASAAIGGAFV
                     IAALAAVPFIQGGAEPVTISLWEWMPAIGASFELLWDPLAALMALIVTGVGTLIHVFA
                     IGYMHGDERFHRFFTNLNLFAASMLTLVLAGNYAMLFLGWELVGLCSYLLIGFWYTRT
                     TAAAAAKKAFIVNRIGDFGFMIGLALIFVVFGTLSFAGVFERAGEELTGGMATAIGLL
                     LLVGAAGKSAQIPLYVWLPDAMEGPTPVSALIHAATMVTAGVYVIARSAPIYEFTPLA
                     SVVVATVGALTAIWAASIALAQRDIKKVLAYSTISQLGFMFLAVGARAYVAGVFHLMT
                     HAFFKALLFLGAGSVIHAMSDEQDMHRMGGLRRKMPVTAITMAIGALALAGIPPLAGF
                     WSKDEILAADFARGGWFYVLWIIGLITALLTAFYIARLWVLVFMGEPRWEESAHPHES
                     PRVMTLPLIALAILSVVGGLVNTPFRLGLEHFLEPSFEGIQVQEAPTDWLTLGVLAGL
                     STLAALAGAGAGILAYNRRRERWRRFEESFDPLWGTWEQAYGVDDLYGRALVLPGRRL
                     AEVTAFSVDAKVVDGAVNGVGRMVRNAGDWARPLQTGFVRNYGVLFLAGALAVVVWLV
                     TGA"
     gene            3086..>4136
                     /locus_tag="VFU96_11500"
     CDS             3086..>4136
                     /locus_tag="VFU96_11500"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEU5113933.1"
                     /translation="MLTALILLPVAAAIVVMLIPARRREIHLPLGIALSVMPLALAGY
                     LFSVFEPAAGFQYVDRVPWYEPWGIDWYLGVDGISLPMVVLTTLLVPIALAASTTITT
                     RTKEFVVYTLLLEAGMIGAFLALDLFLFFVFFEAMLIPMYFIIGIWGTERRVYAAVKF
                     FIYTAFGSALMLAAIIGLALTYASQEGSASFALSDLADLDLSVGTERLFFAAFAIAFA
                     IKVPLFPFHTWLPDAHVEAPTAGSVLLAAVLLKLGTYGLLRFNLTLFPEASVDAIPIM
                     AILAVIGIIYGAVVAIVQPDLKKLVAYSSVSHLGFIVLGTFALTSGGLQGAVIQNVNH
                     GLTTGALFLLVGMIYD"
BASE COUNT          579 a         1337 c         1340 g          880 t
ORIGIN      
        1 gcggttcggt tacgtgtacg agatcaacat gttgcgctgc atcttctgtg gtctctgcgt
       61 ggaggcttgc cccaccgagg caatcaccat gaccagcctc ttcgagatct ccaccaccaa
      121 ccgcgaggac gccatctaca ccaaggaaac tctcctcatg gaccggcggg ggaacgtgcc
      181 ccacatgttc cctgacgacg agctcgccga tctcgacgag ctggacacgg cggatggctg
      241 gctgagggcc accgcaccct ccggggtggc cgcgtacgaa ggggtcaaga tgtgggaggg
      301 aaccccgggt ccggcggact ggtcaccaga gccgtctcag gaggagcgcg accgcaggac
      361 aaccgatggg gaagagaatg gttgagacgg tcatcttcgt gatcgcagcc ctggccgcgc
      421 tgggtggggc ggtgaccgtg gtgctcgccc ggaatccgat ctactcagcg gtagggctcc
      481 tcggcacgct gttctcagtt gcgatcctct atgtggtcca actcgcccac cttgtggccg
      541 cggtccaggt gatcgtctat gccggggcgg tcatgaccct gttcctgttc gtgatcatgc
      601 tgatcggagt ggacaaggaa gaaacgaggg tcgagcctct tccccgtcaa cggttgtggg
      661 tcggtggcct cgccggtgtc gtgatcgtgt tcgcggcggg agtggcgctg ttcggggatt
      721 tcgtctgggt gccggccgcg gccgccacag tcgatcagga agccaccaac gggacgattc
      781 aggcggtggc cgagccgctg ttcaccgact gggtccttgc cttcgaggcc accgcgttgc
      841 tgttgacgat cgccgccgcc ggtgcggtcg tcctggctca gttcaagaga aagcgggcgt
      901 gatggtcccc gtcgggtggt acatggccct ggccgcggcg ctgtttctca tcggcgccct
      961 cggcatgctc ctccggcgaa acgcgctggt catgttcatg tgcatcgagc tgatgctgaa
     1021 cgcggtcaac ctcaccttga tctcggcggg acgggcgatg aacgacctca acggacaggt
     1081 ggcagtcttc ttcatcctcg tggtcgcggc tgccgaggtc gtggtggggc tggccatcat
     1141 cgtcgcaatc ttccgacgac gtgccaccgc gtccgtggac gagctctcgg agctgcgggg
     1201 atgaccgact atgtgtggtt ggtgatcgcc cttccccttg ccggcgctct gttcctccat
     1261 ttcttcgggc gatacgtcaa ggagccgctg agcggctggc tggcctcggc tgcaatcggc
     1321 ggcgctttcg tgattgccgc cctcgccgcg gtgccgttca tccagggcgg tgccgagccg
     1381 gtgaccatct cgttgtggga gtggatgccg gcgatcggtg cgagcttcga gctgctctgg
     1441 gatccgttgg cggctctcat ggccctcatc gtcaccggcg tcggcacgtt gatccatgtc
     1501 tttgccatcg gctacatgca tggcgacgag cgcttccacc ggttcttcac caacctcaac
     1561 ctgttcgccg cctcgatgct cacgctggtg ctcgccggca actacgccat gttgttcctc
     1621 gggtgggagc tggtcggtct ctgctcctat ctgctgatcg ggttctggta cacgaggacc
     1681 acggcagcgg ccgcggccaa gaaggctttc atcgtgaacc gaatcggcga cttcggattc
     1741 atgatcggcc tggcgctgat cttcgtcgtg ttcggcaccc tttcgttcgc cggcgtcttc
     1801 gagcgggccg gcgaggagct caccgggggg atggcaaccg ctatcgggct gctcctgttg
     1861 gtcggcgccg caggcaagtc ggcgcagatc ccgctctacg tgtggctgcc cgacgcgatg
     1921 gaaggcccga ctccggtctc ggccctgatc catgccgcga cgatggtgac cgccggcgtc
     1981 tacgtcattg cccggtccgc ccccatctat gagttcacac cgctcgcctc ggtggtggtc
     2041 gccaccgtgg gcgcgctgac cgcgatatgg gcggcctcca tcgcgctcgc ccagcgcgac
     2101 atcaagaagg tcctcgccta ctcgaccatc tcccagcttg gcttcatgtt cctcgcggtg
     2161 ggggccaggg cgtatgtcgc cggggtgttc cacctgatga cccatgcctt cttcaaggca
     2221 ttgctcttcc tcggcgccgg ttccgtgatc catgcgatga gcgatgagca ggacatgcat
     2281 cgaatgggcg gtcttcgtcg gaagatgccg gtgaccgcca tcaccatggc catcggggcg
     2341 ctggcactgg ccgggatccc gccgttggcg ggcttctggt cgaaggacga gattctcgcc
     2401 gcggacttcg cccgaggcgg ctggttctac gtcctttgga tcatcgggct gataaccgcc
     2461 ctgctcactg ccttctacat cgccaggctc tgggtgctgg tgttcatggg agagccgcga
     2521 tgggaagaga gcgcccaccc ccacgagtcc ccgagggtga tgacgctccc tctcatcgcc
     2581 ctggccatcc tctccgtggt cgggggactg gtcaatactc cgttccggct cgggctcgag
     2641 catttcctgg agccgagttt cgaaggaatc caggtccagg aggctccgac cgactggctc
     2701 acgttgggcg ttctggccgg tctctccaca ctggcagccc tggccggtgc cggggcgggc
     2761 attctcgcct acaaccgcag gcgggagcgc tggcgccgct tcgaggaatc cttcgacccg
     2821 ctctggggga cctgggagca ggcctatggg gttgacgacc tctacgggag ggctctggtg
     2881 ctgccaggtc ggagactggc cgaggtcact gccttctccg tcgacgcgaa ggtggtcgat
     2941 ggcgcggtca acggcgtggg gcggatggtt cgcaacgccg gggactgggc tcggcccctc
     3001 cagaccgggt tcgtccgtaa ctacggggtc ttgttcctgg ccggtgcgct ggcggtagtg
     3061 gtctggctgg tgacaggggc atgagatgct gacggccctg atccttctcc ccgtggctgc
     3121 cgcgatcgtc gtgatgttga tcccggctcg ccggagagag atccatcttc ccctcggcat
     3181 cgccctctcc gtgatgccgc tggcgctggc cggctacttg ttctcggtct tcgagccggc
     3241 ggccggcttc caatacgtcg accgcgtccc ctggtacgag ccgtggggca tcgactggta
     3301 tctcggagtg gacgggatct cgctgccgat ggtcgtgctc accacgctgc tggttccgat
     3361 cgcgcttgcc gcctcgacga cgattacgac ccggaccaag gagttcgtgg tctacacgct
     3421 cctgctcgaa gcgggaatga tcggggcatt cctggctctc gatctcttcc tcttcttcgt
     3481 cttcttcgag gcgatgttga tccccatgta cttcatcatc gggatctggg gcaccgaacg
     3541 ccgggtgtat gccgcggtca agttcttcat ctacacggcc ttcgggtcgg cgttgatgct
     3601 ggcggcgatc atcggcctcg ccctcaccta tgcgtcccag gaaggatctg cctccttcgc
     3661 tctttccgat ctcgccgacc tcgacctctc ggtggggaca gagcggctct tctttgccgc
     3721 attcgccatc gccttcgcca tcaaggtgcc tctcttcccg ttccacacct ggctacccga
     3781 cgcccacgtc gaggcaccga ccgcagggtc ggtgctgctg gccgcggtgc tcctgaaact
     3841 ggggacctac ggccttctcc ggttcaacct caccctgttt cccgaggctt cggtcgacgc
     3901 catcccgatc atggcgatcc tggccgtgat cgggatcatc tacggggcgg tggtggcgat
     3961 cgtccagccc gacctgaaga agctcgtcgc ctactcctcg gtgagccatc tgggtttcat
     4021 cgtgctcggc accttcgccc tcacctcggg ggggctccag ggagcggtca tccagaacgt
     4081 gaaccacggt ctgaccaccg gcgccttgtt cctcttggtg gggatgatct atgacc
//
LOCUS       DASTXO010000542         2779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1653711, whole genome shotgun sequence.
ACCESSION   DASTXO010000542 DASTXO010000000
VERSION     DASTXO010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1653711"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VFU96_11505"
     CDS             <1..588
                     /locus_tag="VFU96_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113934.1"
                     /translation="WGIDTVLTYATSVMDLGVYEHHVADGKRHALYGSLDELREMLIE
                     CLDAAASLSGRPGPGDGARHGTDPTAPAPEDLKQIVAYDVAGTARLLEAAWGEEEVHL
                     VDRLPRDLFGFLLDDPAHPKFELMDADRWYVAHGFLLEGFALGDSAWESLAFRLWLTR
                     VLAYTTNQAGMGFDAAMTYLEATILRYEAMAAGSS"
     gene            complement(558..1853)
                     /locus_tag="VFU96_11510"
     CDS             complement(558..1853)
                     /locus_tag="VFU96_11510"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5113935.1"
                     /translation="MTTTRSTLIAIQHGRSLPGYGGKCLVPRIPGIAESPVRARPFRP
                     AVATLLFLKLVANIGFRFVYPFLPAIARGLGIDLTQAGALVSVRWAGALTSPAVMSFS
                     GNRGRSRRLLVAGLALFSIGSIVTAWTGVYVGAVIGFALVGLAKPMIDISSQVYVSER
                     VAYEERARYLGILEIAWAGGLLLGAPLAGWLIDNWSWEAPFWVVGSLGILGIGLAMFF
                     LDRRDDQVPTVMAADSPGRQVALVLVTVALFGFSHESLLVVLGAWLEGSFALSLIALG
                     GVGTLIGLSELAGEVSMAAFTDRIGKRNSLALGLAVGALALLVLSRVSEQLVPAMAVL
                     VVVGIAVEFGIISAIPLTTELRPRARARLLSLLIVASGSGRLVGDLVAPRVFTAGGMQ
                     SVTVMAAIAMLIALAVVLVGVHEVEAGSGDQEDPAAIAS"
     gene            1822..2262
                     /locus_tag="VFU96_11515"
     CDS             1822..2262
                     /locus_tag="VFU96_11515"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU5113936.1"
                     /translation="MAMSVLLVVVIAVLGGLAGALQSQMLGVMEERVGTLASTFVTYA
                     GGGLAVTILMLVFQGSRLSELKALPWWVYGAGIMGLVIVAALGVSVASLGLAAGLTLF
                     TASILVMGAVIEHLGWFGDGRSFDLIRLIGVALVIGGTWLVVRG"
     gene            complement(2269..>2779)
                     /locus_tag="VFU96_11520"
     CDS             complement(2269..>2779)
                     /locus_tag="VFU96_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU5113937.1"
                     /translation="DGERPSALAWIGVALAIPAIVLSSWVADPGETPKGRFGYGLAAG
                     LGFGGFTAIIGFTDPESNLLPLITSRAATMLAVLLLAGLGVWKLVGFGAVPQRVVLGN
                     AVLDVSGNVALLLAVRAGSLALAAVAASFYPAVTVILARLVNAEHLRKRQIVGLGLTI
                     VAMASIALG"
BASE COUNT          521 a          943 c          905 g          410 t
ORIGIN      
        1 tggggaatcg acaccgtgct gacctacgcc acctcggtca tggacctcgg cgtatacgag
       61 catcacgtcg ccgatgggaa gaggcacgcg ctctacggat cactcgacga gctgcgggag
      121 atgctcatcg aatgcctcga tgcggccgcg tcgttgtcag ggcgacccgg gccgggagac
      181 ggtgcaagac acggcaccga tccgaccgca ccggccccag aggacctgaa acagatcgtt
      241 gcctacgatg tcgccgggac tgcccgtctg ctcgaggccg cgtggggcga ggaggaggtc
      301 cacctggtcg accggctccc ccgcgacctg ttcggttttc ttctcgacga tcccgcccac
      361 ccgaagttcg agctcatgga tgctgatcgc tggtatgtgg cgcacggctt cctgctggaa
      421 ggtttcgctc tcggcgattc ggcgtgggag tcgctggcct tccggctgtg gctgacccgg
      481 gtgctggcct acacgaccaa tcaggccggc atggggttcg acgccgcgat gacctatctg
      541 gaggccacca tcctgcgcta cgaggcgatg gcggccgggt cctcctgatc cccggagccg
      601 gcttcgacct cgtggacccc cacgagaacc acggccagtg cgatcagcat tgcgatcgcc
      661 gccatcacgg tgacggactg catccctcca gccgtgaaca cccgaggcgc caccaggtct
      721 ccgacgagcc gacctgaacc gctggcaacg atgagtagcg agagcagtcg agcgcgggca
      781 cgtggccgta gctcggtggt caaagggatg gcagagatga tcccgaattc gacggcgata
      841 cccacgacga cgaggacagc catcgccggg accaactgct cagacaccct cgagagcacc
      901 aggagcgcca gcgcgccgac cgccagtccg agagcgaggg agtttcgctt gccgatgcga
      961 tcggtgaagg cggccatcga cacttcgcca gccaactcgg aaagcccgat gagagtcccg
     1021 actccaccga gggcgatcaa gctcagcgcg aagctgccct cgagccacgc cccgagcacg
     1081 accaagagcg actcatgcga gaagccgaag agcgccaccg tcaccagaac cagagccacc
     1141 tgccgaccag ggctgtccgc cgccatgacc gtcgggacct ggtcgtctct ccggtccaga
     1201 aagaacatcg ccaggccaat gccgaggatc ccgagcgacc cgacgaccca aaacggggcc
     1261 tcccagctcc agttgtcgat cagccaccct gccagcgggg cacccaggag gagcccgccc
     1321 gcccaggcga tctccaggat tcccaggtac cgcgcccgct cctcgtacgc cacccgctcc
     1381 gacacataga cctgcgagct gatgtcgatc attggcttgg ccaggcccac caatgcgaaa
     1441 ccgatcaccg ccccgacgta caccccggtc caggccgtga caatcgaccc gatcgagaag
     1501 agggccaatc cggcgaccag gaggcgacga ctccggcccc ggttcccgga aaacgacatc
     1561 acggcgggcg aggtcagcgc ccctgcccag cggactgata ccaacgcgcc ggcctgggtc
     1621 agatcgatgc ctagcccccg cgcaatcgcg ggcaagaagg gatacacgaa ccggaagccg
     1681 atgttggcca ccagcttcag aaagagaagg gtggcgacgg cggggcggaa cggccgtgct
     1741 cggacgggtg actcggctat gcccggaatc ctagggacga ggcactttcc cccatatccg
     1801 gggagggacc gaccatgctg gatggcgatg agcgtgctcc tggttgtcgt gatcgcagtc
     1861 ctcggcggtc tggccggggc gcttcaatcc cagatgctcg gagtgatgga ggagcgagtg
     1921 gggacgttgg cgtccacctt cgtcacctac gccgggggcg gactggcggt gaccatcctg
     1981 atgctggtct ttcaggggag ccggttgtcg gagctgaagg ccctgccctg gtgggtgtac
     2041 ggcgccggca tcatggggct ggtgatcgtg gcagccctcg gggtcagcgt ggcaagtctt
     2101 ggccttgccg cgggcctgac actcttcacc gcgtccatcc tcgtcatggg agcggtcatc
     2161 gagcatttgg gctggttcgg ggacggccgc tccttcgacc tcatccggct gatcggagtg
     2221 gccctggtga tcggtgggac ctggctcgtg gtccgaggct gatcgagctc accccagggc
     2281 gatcgatgcc atcgccacga tggtgagccc gagcccgacg atttgccgtt ttcggagatg
     2341 ctcggcgttc accaaccggg cgaggatcac cgtgaccgcc gggtagaagg aggcggcgac
     2401 ggcggccagg gcaagcgaac ccgcccgcac ggcaagcagc agggcgacgt tgccgctcac
     2461 gtcgaggacg gcattcccca gcaccacccg ctgcggcacc gcgccaaacc cgacgagttt
     2521 ccagactccg aggccggcga gcaacaacac cgccagcatc gtcgccgccc gagaagtgat
     2581 gagcgggagc agattcgact caggatcggt gaacccgatg atcgcggtga aaccgccaaa
     2641 ccccagcccg gcggccaacc cgtatccgaa tctgcccttt ggcgtctcgc cggggtccgc
     2701 cacccaggaa ctgagaacga tcgccggtat cgccaacgcc acgccgatcc aggcgagcgc
     2761 actgggtcgc tcgccatcg
//
LOCUS       DASTXO010000543         2177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_549198, whole genome shotgun sequence.
ACCESSION   DASTXO010000543 DASTXO010000000
VERSION     DASTXO010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549198"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /locus_tag="VFU96_11525"
     CDS             complement(<1..1063)
                     /locus_tag="VFU96_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5113938.1"
                     /translation="MTMDQPPSARVLVVDDQPANVRLLEAMLVTRGYDVMTASSGEEA
                     LALIAQSEPDLVLLDIVMPGMDGYEVCRMIRERIETAYLPVVMVTASGDEQKVKALEV
                     GADDFLTKPINKSELLARVASLARIKRYQDTIKRQTDELAAWNRELETRVEIQVSQLE
                     RMGRLRRFLSPQVAELILDSGVESVLESHRRDIVVVFCDMRGFTTFAEASEPEEVMGV
                     LREYHEALGDLIFRFEGTLERFAGDGLMVFFNDPVRCEDGPLRAIRMSVAMRTRVHAL
                     SEGWARQGHDLALGIGIAQGYATLGKIGFDSRLDYAAIGTVTNLAARLCADAEPWQIL
                     VTERVLAAAGNLVVGEDAGS"
     gene            complement(1060..1443)
                     /locus_tag="VFU96_11530"
     CDS             complement(1060..1443)
                     /locus_tag="VFU96_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5113939.1"
                     /translation="MSQGRILVVEDNPKNLKLVRDVLTFSGYEVLEASSGEDGVRLAK
                     EASPDLILMDLQLPGIDGAEALRQIRVNDKKQGVPVIAVTAFAMNEDRARAFESGFDG
                     YVEKPISARNLPQMIEDFLGMGGPT"
     gene            complement(1440..>2177)
                     /locus_tag="VFU96_11535"
     CDS             complement(1440..>2177)
                     /locus_tag="VFU96_11535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5113940.1"
                     /translation="LTNAVKFTDDGGSVVMRARRRTSEIDITVTDTGVGVPEADRERI
                     FESFQQGGRGSSREEGTGLGLTLSRRIVELLGGRMWLETEVGVGSTFGFSLPGREGAE
                     QTGTGARAPVVGDVVVIEDDRLSIDLLTAYLSDAALRVTTARDGQSGLEAVRRVRPDV
                     VLLDIRLPGIDGWSVLQALKAGEETREIPVIVVSIVDERARGVALGAAAYLVKPVGRD
                     DLLAALQSVGAAVPAPDDGEGTEASTT"
BASE COUNT          378 a          757 c          650 g          392 t
ORIGIN      
        1 gcgagccagc gtcctcgccg acgacgaggt ttccggctgc agcgagcacg cgctcggtca
       61 cgagaatctg ccacggctcg gcgtccgcgc acaatcgggc ggcgagattg gtcaccgtgc
      121 cgatcgccgc atagtccaaa cggctatcaa atccgatctt cccgagggtc gcgtagccct
      181 gggcgatgcc gatgcccaag gccagatcat ggccctgtct cgcccagccc tcggagagag
      241 cgtggacgcg agtccgcatc gcgacgctca tacggattgc ccgcagggga ccgtcctcgc
      301 atcgaactgg atcgttgaag aacaccatca ggccgtcacc tgcgaacctc tcgagggttc
      361 cttcgaaccg gaagatgagg tctccgaggg cctcgtggta ctccctgagg acgcccatca
      421 cctcttcagg ttcactggcc tcggcgaacg tggtgaagcc ccgcatgtcg caaaacacca
      481 cgacgatgtc gcggcggtgg ctctcgagga ccgactcgac tcccgagtcg aggatcagct
      541 ccgcgacctg gggggacaga aaccgacgca accggcccat ccgctcaagc tgggagacct
      601 gtatctcgac tctcgtctcc agctcgcggt tccaggcggc cagctcgtcg gtctggcgct
      661 taatggtgtc ctgatagcgc ttgatcctgg ccagcgacgc cacgcgggcc agcagctcgc
      721 tcttgttgat cggcttggtc aggaagtcgt ccgccccaac ctccaacgcc ttgaccttct
      781 gctcgtcgcc gctggcggtc accatgacga cgggaaggta tgccgtctcg atccgctctc
      841 ggatcatgcg gcatacttcg tagccgtcca tgcctggcat cacgatgtca aggaggacga
      901 gatccggctc ggattgggcg atcagcgcca gtgcctcctc gcctgaggac gcggtcatga
      961 cgtcgtagcc ccgcgtcacg agcatcgcct ccagcaaccg gacgttggct ggctggtcat
     1021 ctacgacgag cacccgtgcg ctgggaggtt ggtccatagt catgtcgggc ctcccatgcc
     1081 gaggaagtcc tcgatcatct gcggcaggtt gcgtgcactg atcggtttct cgacgtaccc
     1141 gtcgaatccg gactcgaacg cccgtgctcg gtcctcgttc atagcgaagg cggttacagc
     1201 gatgacgggg acgccctgtt tcttgtcgtt gacgcgaatc tgccgcagcg cctcagcacc
     1261 gtcgattcct ggcagttgaa ggtccatcag gatcaggtcc ggagacgcct ccttcgctag
     1321 acgcacgccg tcttcccctg agctcgcctc gagcacctca tagcccgaga aggtgaggac
     1381 gtccctgacc agcttgagat tcttcggatt gtcttcgact acgagaattc ggccctggct
     1441 catgtcgtac tcgcctcggt tccttctccg tcgtctggtg ccgggaccgc agcacccacg
     1501 ctctgcagcg cagccagcag gtcgtcccga cccacgggct tcaccaggta tgccgccgcc
     1561 cccaaggcga ctccacgcgc ccgctcgtcg acgatggaca ccacaatcac cgggatttcg
     1621 cgggtctcct ccccggcctt gagcgcttgg agcaccgacc acccgtcgat ccctggcagg
     1681 cggatgtcaa ggagcacgac gtctggacgc acccgccgta ccgcctcgag tccagactgc
     1741 ccgtcacggg cggtggtcac tcggagtgcg gcatcggaca gataggcggt caacaggtcg
     1801 atcgagagcc ggtcgtcctc gatcacgacc acatcaccga ccaccggcgc gcgcgcaccc
     1861 gtaccggtct gctcggcgcc ttcgcggccg ggcaacgaga agccgaacgt gctgccgact
     1921 cctacctcgg tctcaagcca catccggccg ccgagcagct cgacgatccg acgcgacaag
     1981 gtgaggccca atccggtgcc ctcctcgcgt gagctgccgc gtccgccctg ttggaacgac
     2041 tcgaagatcc gctcacggtc ggcctcgggg acaccaacac cggtgtccgt gacggtgatg
     2101 tcgatctccg aagtcctacg ccgagcacgc atcacgacgg acccgccatc gtcggtgaac
     2161 ttgactgcat tcgtgag
//
LOCUS       DASTXO010000544         3493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1543659, whole genome shotgun sequence.
ACCESSION   DASTXO010000544 DASTXO010000000
VERSION     DASTXO010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543659"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..515
                     /locus_tag="VFU96_11540"
     CDS             120..515
                     /locus_tag="VFU96_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEU5113941.1"
                     /translation="MFIASLPDERRSDLERVDAVLSKVMRGHSRSLWGGKFWGGSEQS
                     IIGYGDHTYQRSDKKTVEWFIVGLAPQKNYNSIYINAADDDGYVIERYADRLGKVKVR
                     GAAITFKKADELDLEVLEEVATLARDRLS"
     gene            complement(512..2542)
                     /gene="ligA"
                     /locus_tag="VFU96_11545"
     CDS             complement(512..2542)
                     /gene="ligA"
                     /locus_tag="VFU96_11545"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018174050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU5113942.1"
                     /translation="MPEAASRIEELRSQVNEHLYRYHVLDDPIISDAQFDALIAELRR
                     IEEENPELVTPDSPTQRVGAPVGNLFAPVRHLRPMFSLDNADSSEMLLAWEQRMERQL
                     GRPPVGYVAELKIDGLAVVLTYRDGVLSTGATRGDGVTGEDVTANLRTIKSVPLRLLG
                     RDHPAVLEVRGEVYMPEKAFEELNRRQAEAGDRIFANPRNAAAGSVRQKDPTVTASRN
                     LAIWVYQLGLVEGGPGFETHAETMQYLRDAGFRTNPASERLSAIDEVLGYVERAEKGR
                     HETGYQTDGVVIKVDALPDQDSLGFTSKAPRWAIAYKFPPEEQVTLLKAIQINIGRTG
                     AATPFAVLEPVFVGGATVGMATLHNADQVALKDVRVGDQVIVRRAGDVIPEVVGPVVA
                     ARTRKLRKWSMPASCPFCGNPIVRADGEAVARCTGGFECPSRVREWLFHFASRGGMDI
                     EHMGYKTIDLLLKDGLITDPADIFTFDVGQLRGREGWGEISVNNLSTAIDAAKDRPVA
                     RLLTALGIRHVGGTIARTLVRHFKSLPALMAATEEEIAQVEGIGPTIAKGVAEWAADP
                     VNRDLVEKLGKAAVRLEDEVEAGVSGELLAGATFVVSGTVEGFTREEAQAAIEGRGGK
                     ATSSVSSKTTALIVGDSPGASKSRKAEELGVPIIDGDTFKEMLEKGLSVLGG"
     gene            complement(2568..3074)
                     /locus_tag="VFU96_11550"
     CDS             complement(2568..3074)
                     /locus_tag="VFU96_11550"
                     /inference="COORDINATES: protein motif:HMM:NF042466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA methyl transferase PRC-barrel
                     domain-containing protein"
                     /protein_id="HEU5113943.1"
                     /translation="MGLRTAAKPESMDICFVGQRDYRGFLRDTAPEVFEPGPLMASDG
                     TDLGRHDGIVGFTVGQRRGLGIAVGEPRFVVRIDSASSTVVLGRRDELAVAGVGLRHV
                     TWTDGHPFSGQVMAQYRAHGEPVEATLHEDRLLFAKAQEAVAPGQTVAFFDEDRVLGG
                     GLIAETFV"
     gene            complement(3090..>3493)
                     /locus_tag="VFU96_11555"
     CDS             complement(3090..>3493)
                     /locus_tag="VFU96_11555"
                     /inference="COORDINATES: protein motif:HMM:NF015042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEU5113944.1"
                     /translation="PTAGCCTVSDAEDARRVTAQLDIPYYVLDYTEEFRDGVVHRFVR
                     DYGSGRTPNPCIECNRTVKFDRLLNGLSDFDSEVLVTGHHARNRFVDGSWQLHRGQDR
                     AKDQSYVLSMLTQAELSRVRLPVGELPKTEL"
BASE COUNT          637 a         1153 c         1091 g          612 t
ORIGIN      
        1 accactatga caccattatt ggtgtctggc aagacaccaa atcgacacca aagggaatcg
       61 actagattgg acggtgccga aaggatctcc gcatcgagaa gtccacgacc gatcccgacg
      121 tgttcattgc ctccctgccc gacgagcgcc gctccgatct ggagagagtg gacgccgtgc
      181 tcagcaaggt gatgagaggg cactctcgct cgctgtgggg aggcaagttc tggggtggga
      241 gcgagcagag catcatcggg tatggcgacc acacctacca gcgctcggac aagaagacgg
      301 tcgagtggtt catcgtcggt cttgcccccc agaagaacta caacagcatc tacatcaatg
      361 cagccgacga cgacggctac gtgatcgagc gctacgccga ccggttgggc aaggtgaagg
      421 tccgaggcgc cgccatcacc ttcaagaagg cagacgaact ggatctggaa gtactcgagg
      481 aagtggccac cttggcccgg gaccggttga gttagccgcc gaggaccgag aggccctttt
      541 ccagcatctc cttgaaggtg tcgccgtcga tgatgggcac ccccaactcc tcggccttgc
      601 gcgacttgga agcgcccggc gaatcaccca cgatcagagc ggtggtcttc gacgagacgg
      661 aactggtcgc cttgcccccg cgcccctcga tggcggcctg cgcctcctct ctggtgaaac
      721 cctccacggt gcccgacacg acgaaggtag ccccggccaa caactcgccg gagacccctg
      781 cctccacttc gtcctcgagc cgcaccgcgg ctttgcccag cttttcgacg aggtcgcgat
      841 tgactgggtc ggctgcccac tccgccactc ctttggcgat ggtgggcccg atgccctcga
      901 cctgagcgat ctcctcctcg gtcgccgcca tcaaggccgg gaggctcttg aagtgccgga
      961 ccagggtccg ggcgatggtg ccaccgacat ggcgaatccc gagagcggtc agcaaacgtg
     1021 caactggtcg gtccttagcc gcatcgatgg ccgtgctcag gttgttcacc gagatctcgc
     1081 cccaaccctc gcgaccccga agttgtccca catcgaaggt gaagatgtcc gccgggtcgg
     1141 tgatcaaacc gtctttgagg aggaggtcga tcgtcttgta gcccatgtgc tcgatgtcca
     1201 tccctccccg ggatgcgaag tggaacagcc actcacggac acgagacggg cactcgaagc
     1261 caccggtgca acgagccaca gcttcgccat cggcgcggac gatcgggttg ccacagaagg
     1321 ggcagctggc cggcatcgac cactttctga gcttgcgcgt gcgggcagca accacgggcc
     1381 cgactacctc cgggatgaca tcgccggcgc ggcggacgat gacctgatcc cccacccgca
     1441 catccttgag cgccacctga tcggcgttgt gcaacgtcgc catgcccacc gtcgctccac
     1501 ccacgaacac cggttcgagc actgcgaatg gggtggcggc gccggtgcgc ccgatgttta
     1561 tctggatcgc cttgagcagg gtgacctgct cctcaggcgg gaatttgtag gcgatcgccc
     1621 acctcggcgc ctttgaggtg aaacccagcg aatcctgatc gggcaacgcg tcgaccttga
     1681 tcaccactcc atcggtctga tatccggtct cgtgacggcc cttttccgcc ctctccacgt
     1741 agccgagcac ttcgtcgatg gcggacaatc gctcgctagc cgggttggtc ctaaagccgg
     1801 cgtcgcgcag gtattgcatg gtttcggcat gggtctcgaa cccagggccg ccttcgacca
     1861 ggcccaattg gtagacccag atcgccaggt ttcgcgacgc ggtgaccgtg gggtccttct
     1921 ggcgcaccga gccggccgcg gcattccgcg gattggcgaa gatccgatcc cccgcttcgg
     1981 cctgtcgccg attcagctcc tcgaaggcct tctcgggcat gtagacctcg ccgcgtacct
     2041 ccaggacagc agggtggtcg cgtcccagca gccgaagggg caccgatttg atggttcgca
     2101 ggttcgccgt cacgtcctcg cccgtcactc cgtcgcctct cgtggcaccc gtggagagga
     2161 caccatcgcg gtaggtgagg accacggcca ggccgtcgat cttgagctcg gcaacgtagc
     2221 cgactggggg ccttcccagc tggcgctcca tgcgctgctc ccaggcgagg agcatctcgg
     2281 aggagtcggc gttgtcgagc gagaacatag gccgaagatg ccgaaccggt gcaaagaggt
     2341 tcccgaccgg cgccccgaca cgctgggtcg ggctgtccgg ggtgaccagc tcggggtttt
     2401 cctcctcgat gcggcgcagc tcggcgatca gtgcgtcgaa ctgcgcatcc gagatgatcg
     2461 ggtcgtctag cacgtgatag cggtacagat gctcgttgac ctgagagcga agctcctcga
     2521 tccggctcgc agcttcgggc atggtgccgg acgttaccgc cctggggtca cacgaaagtc
     2581 tcggcgatca aaccgccacc gagcactcga tcttcgtcga agaaggccac cgtctgaccc
     2641 ggggcaaccg cctcctgtgc cttcgcaaac agcagccgat cctcgtggag tgtggcctcg
     2701 accggctcgc catgggcccg atactgggcc atgacctgac ccgagaacgg gtgtccatcg
     2761 gtccacgtga cgtgtctcag ccccacgccg gcgacggcca gctcatcgcg acgtcccagg
     2821 acaactgtcg acgacgcgga gtcgatgcgg acgacgaacc tcggctctcc gacagcgata
     2881 ccgaggccac gccgctgacc gacggtgaac ccgacgatcc cgtcatgacg ccccaggtcc
     2941 gtgccatcgc tggccatcag cggccccggc tcgaagacct caggcgcagt gtcgcggagg
     3001 aagccccggt agtcgcgctg gccgacgaag cagatgtcca tcgactcggg cttggctgcg
     3061 gtgcgaagcc ccatctcggt ggcgaccgct cacagttcag tcttggggag ctcacctacc
     3121 ggcagcctca ccctcgagag ctcggcctgg gtcagcatcg agaggacata ggactgatcc
     3181 ttcgcgcggt cctgtcctcg atggagctgc caggagccgt cgacgaaacg gtttcgggca
     3241 tggtgcccgg tgaccaggac ctcggaatcg aaatcggaaa ggccgttgag cagccggtcg
     3301 aatttgactg tccggttgca ctcgatgcac gggtttgggg tgcgacccga gccatagtcc
     3361 cgcacgaagc ggtggaccac accatcacgg aactcctcgg tgtaatcgag cacgtagtag
     3421 gggatgtcga gttgggctgt gacccttcgt gcatcctcgg catcggacac ggtgcagcac
     3481 ccggctgtcg gtg
//
LOCUS       DASTXO010000545         2033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_990697, whole genome shotgun sequence.
ACCESSION   DASTXO010000545 DASTXO010000000
VERSION     DASTXO010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990697"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFU96_11560"
     CDS             <1..197
                     /locus_tag="VFU96_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113945.1"
                     /translation="DPALEAYVIETFAPDGSREDPCIDIPTAELEFFSDGNSDNAYHY
                     FGAEDTTTTTTFPVTTTVSG"
     gene            266..1183
                     /locus_tag="VFU96_11565"
     CDS             266..1183
                     /locus_tag="VFU96_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113946.1"
                     /translation="MNQPSPARLLKEWKRDLADGGDGVDLDVYGVTSSMVYWADVMYA
                     AATDAGGSSHESIEYEGIESGALTEVDESYIAEASGEEKDFIESMIANYDLDHPDEPA
                     PEAAPDAGAEARVAAASALEAIPLPWFVKRPLMRLLLRDVHHYLFNTSHTPRAGSTFK
                     VRDEVRKRFGADLGAVDGGPHVVVAHSLGSVIAYDCLKRVAGTKKVDLLVTIGTPLGM
                     SEVQHNLKPEWSKDDGYPGELPRWVNVVDRLDPVCVADPIIANDYMRKKASVVVDQVV
                     SNGGVMRHPSGKYFRQAVVQDAVREGLGL"
     gene            1193..>2033
                     /locus_tag="VFU96_11570"
     CDS             1193..>2033
                     /locus_tag="VFU96_11570"
                     /inference="COORDINATES: protein motif:HMM:NF042664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HEU5113947.1"
                     /translation="MSFFDSPLGEQLRTSLDAGKPEDIAVAVGAIEGKVWSGKVQPDQ
                     VEEVLAELQRQRQVQHVIDIADAATAVGSITTSGRRRYCQMLIESGALHAGELACAEL
                     LDLAEDAKERGEALGLLGRIRKQRYVTSRRSEDLIGAIAAYQFAYAGGGDPLWHGINL
                     LALTTRAHRDGILVPQPVDTDALTRTLLDEVRQTPPELKEIWERTTEVEALVALGDKA
                     SVDEAVSVAQQLASSKSVRPFELESLRRQLADIWELEPHHPVLVAIAAQKLQLGARAH
                     VELP"
BASE COUNT          375 a          650 c          685 g          323 t
ORIGIN      
        1 gagatcccgc cctcgaggcg tacgtgatcg agaccttcgc gcccgacggg agccgggagg
       61 acccgtgcat cgacataccg accgcggagc tcgagttctt ctccgacggc aacagcgaca
      121 acgcatacca ctacttcggg gccgaagaca ccactaccac taccacgttc cctgtcacga
      181 cgaccgtctc cggctgagcc ggctgcgtag gctcgacagt cgaaaggggg actcgatggc
      241 ccatgtgact ttcgttcacg gaatcatgaa tcagccttcg ccggcacgtc tgctcaagga
      301 gtggaagcgg gacctggccg acggcgggga cggcgtcgat ctcgacgtct acggggtgac
      361 ctcgtcgatg gtgtactggg ccgacgtcat gtacgccgct gccaccgatg ccgggggcag
      421 ctctcacgag tcgatcgagt acgagggcat cgagtcgggc gccctcaccg aggtcgacga
      481 gtcgtacatc gccgaggcca gcggagagga gaaagacttc atcgagtcga tgatcgccaa
      541 ctacgacctc gaccatcccg acgagccggc acccgaggcc gctccggacg cgggggcaga
      601 ggccagggtg gcggcggcct cggcgttgga ggcgatacct ctgccctggt tcgtcaagcg
      661 gccgctgatg cggctgctcc tacgcgacgt ccaccactac ctcttcaaca ccagccacac
      721 accccgagcg ggctccacct tcaaggtccg cgatgaggtg aggaagcggt tcggcgccga
      781 cctcggggcc gtcgatggtg ggcctcacgt ggtggtggcg cacagcctcg gctcggtgat
      841 cgcgtatgac tgtctgaaac gggttgccgg caccaagaag gtggacttgc tggtcacgat
      901 cggcacgccg ctcggcatgt ccgaggtcca gcacaacctg aagccggagt ggtcgaagga
      961 cgacggctac cccggcgagc tgccccgttg ggtcaacgtg gtcgatcgtc tcgacccggt
     1021 ttgtgtggcc gacccgatca tcgccaacga ctacatgcgg aagaaggcaa gcgtcgtggt
     1081 cgaccaggtc gtctcgaatg gcggggtgat gcgccatccg tccggcaagt acttccgaca
     1141 ggcggtcgtt caagatgccg tgcgtgaagg cctgggtctc tgagggccgg ggatgtcgtt
     1201 cttcgactcg ccacttggtg agcagctccg caccagcctc gatgcgggca agcccgagga
     1261 catagccgtc gcggtagggg cgattgaggg gaaggtgtgg tcggggaaag tgcagcccga
     1321 tcaggtcgag gaggtcctgg cagagctaca gcgacaacgg caggttcagc acgtcatcga
     1381 catcgccgac gcggccaccg cggtcggctc gatcacgacg tcgggccgtc gccgctactg
     1441 ccagatgctg atcgagtcgg gggccctcca tgccggcgag ctcgcctgcg ccgagctgct
     1501 cgatctggcg gaggatgcga aagagcgggg ggaggcactg ggcctgctcg ggcggatccg
     1561 caagcagcga tacgtgacca gtcgccgatc cgaggatttg atcggcgcaa ttgccgccta
     1621 tcagttcgca tacgcgggag gtggcgaccc gctctggcat ggcatcaatc tcctcgccct
     1681 caccacccgg gcccatcgtg acggaatcct ggtcccccaa ccggtcgaca cggacgctct
     1741 gacacgcacc ctcctggacg aagtcaggca gacgccaccc gagttgaagg agatctggga
     1801 gcgcaccacc gaggtcgagg ccctggtcgc cctgggcgac aaggcgtcgg tggacgaagc
     1861 ggtgtcggtg gcacagcaac tcgccagtag caagagtgtc cggccgttcg agctggagag
     1921 ccttcgccga cagctggccg acatatggga gctcgagcca catcacccgg tgctggtcgc
     1981 catcgcggcg caaaagctcc aactcggagc acgagcccat gttgagctgc ccg
//
LOCUS       DASTXO010000546         4244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_990698, whole genome shotgun sequence.
ACCESSION   DASTXO010000546 DASTXO010000000
VERSION     DASTXO010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990698"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /gene="sucC"
                     /locus_tag="VFU96_11575"
     CDS             complement(<1..1046)
                     /gene="sucC"
                     /locus_tag="VFU96_11575"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEU5113948.1"
                     /translation="MKIHEYQAKQIMAARGIAVPMGRMATTVEEAVAAVRPLIEESGN
                     PVVVLKSQIHAGGRGKGTFKEHPDLRGVNVITDGVEGGPEAAEDRVRDLAAKMLGSTL
                     VTVQTGPEGKVVSRLYVEQGIDIAREIYLAILLDRSVSRNIVMASTEGGMEIEKVAEE
                     TPDKILREEIDPAIDLADFQANALAHGLGIESDTTAAFIRFVKALDRAATEMDTDLIE
                     INPLVITKGGQVMALDGKMAFDDNALFRHPDVAEMRDETEEDPAELAAKEAGLNYITL
                     DGTIGCLVNGAGLAMATMDTIKHVGGEPANFLDVGGGADEQQVTTAFQIITKDPAVKG
                     IFVNIFGGIMRCDV"
     gene            complement(1056..1451)
                     /locus_tag="VFU96_11580"
     CDS             complement(1056..1451)
                     /locus_tag="VFU96_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEU5113949.1"
                     /translation="MPRRILIAKPGLDGHDRGAKVIARALRDAGFEVIYSGLHQTPAQ
                     IAEAAVQEDVDAVGLSSLSGAHKTLFPQVVEELAARDATNVVVFGGGVIPEADFQALL
                     DAGMATIFTPGTPLDLITSWAESNVPAKI"
     gene            complement(1475..2125)
                     /locus_tag="VFU96_11585"
     CDS             complement(1475..2125)
                     /locus_tag="VFU96_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113950.1"
                     /translation="MSPVLVEPDGLIAPPARPRRSPGPAHWVSAAIVGVLATATLAIG
                     ERGGMDLMTQSDLRLIESHLEAWYGGDFDGAQALRAPERLRTGPSEQRARDEVQYQQT
                     LVATTDILECVPLPPSTIRCDVAYSNVLNEAVQKPPAVVSQQFGVEDGRLLFVAGPYL
                     EDETLTASFRKFANLLFPADYQAACVEEPNFQEPICAEFKLTHLDDWASWHRIEGG"
     gene            2185..3282
                     /gene="tal"
                     /locus_tag="VFU96_11590"
     CDS             2185..3282
                     /gene="tal"
                     /locus_tag="VFU96_11590"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEU5113951.1"
                     /translation="MSASNLHRLAAAGVSIWSDQISKTMLDSGELARRIVEDAVTGVT
                     SNPTIFAGAIVGSSDYDDLLRELKSADVSTEEIAKTLMATDLKRACDDLIGVFEKTDG
                     TDGFVSVEVAPTLAGDTEASVAEARDWVKQIDRPNLLVKIPATPEGIPAIRSLLAEGI
                     SVNITLIFSLERYGEVMDAYLGGLEEYVASGGDPGRVASVASFFVSRVDTEVDKRLDT
                     IGTPEALALKGVTAIANARVAYGRFLEAFGSDRYAALSNQGAREQKPLWASTSTKNPD
                     YPDLLYVENLVAPHTVNTMPLETIDAYQDHGNASPEAFTEADIERASADLDRLGEVGV
                     DYDDVVQTLEDEGVDKFTKSWLELLEDIEKA"
     gene            3359..4096
                     /locus_tag="VFU96_11595"
     CDS             3359..4096
                     /locus_tag="VFU96_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113952.1"
                     /translation="MGQALFSTDHLEQRMLNRQAEISRLIAEQLVDLEEIDYRQVATA
                     DGCRSLSEWTASRLDLSPESAKTMVRTMRRTVDRPDLREALISGAISFDRLEALSRIP
                     EHVGHLEHLDVAGVRRQAAMRARISVEDEYRTSSDQFFVMQPSLDESWWKLWGGLDGA
                     TGALVDKTLSAAADDLPLLPDGTRGDSAWLKAIALADLCLSDDPPPAQLSVFIDASQA
                     TPSNGKAGIELEAGAQDRPRSPRSNPL"
BASE COUNT          782 a         1364 c         1345 g          753 t
ORIGIN      
        1 atgacatcgc aacgcatgat gcccccgaag atgttgacga agatgccctt gaccgcagga
       61 tccttggtga tgatctggaa cgcggtcgtg acctgctgct cgtcggctcc gcccccgacg
      121 tcgaggaagt tggccggctc accaccgaca tgcttgatcg tgtccatcgt cgccattgcc
      181 aggccggccc cgttgacgag acatccgatc gtcccgtcca gcgtgatgta attgagcccg
      241 gcctcctttg cagccaactc ggcagggtct tcctcggtct cgtcacgcat ctcggcgaca
      301 tcggggtgac ggaagagcgc gttgtcgtcg aatgccatct tgccgtcgag cgccatgacc
      361 tgaccccctt tggtgatgac cagggggttg atctcgatca gatcggtgtc catctcggtg
      421 gcagcgcggt ccagcgcctt gacgaaccgg atgaaggctg cggtggtgtc gctctcgatg
      481 cccaggccgt gagcgagtgc gttggcctgg aagtcggcaa ggtcgatcgc cggatcgatc
      541 tcctcccgca ggatcttgtc cggggtctcc tcggcgacct tctcgatctc catcccgcct
      601 tcggtagagg ccatgacgat gtttcggctc accgatcggt cgaggagaat ggcgagatag
      661 atctcacggg cgatgtcgat cccctgctcg acgtacaacc ggctcaccac cttgccctcg
      721 ggaccggttt ggacagtcac cagcgtcgag ccgagcatct tcgctgccag gtcccggacg
      781 cgatcctcgg cggcctcggg cccaccctcg accccgtcgg ttatgacgtt gacgccgcgc
      841 aggtcagggt gctccttgaa ggtccccttg cctctgcccc cggcatggat ctgcgacttg
      901 agaaccacga ccgggttccc cgactcctcg atgagcggcc gcaccgcggc gacggcctct
      961 tcgacagttg tcgccatccg acccatgggt acggcgatgc cccgggcggc catgatctgc
     1021 ttggcctggt actcgtggat cttcaaaggg ttctcctaga tcttggccgg gacattggac
     1081 tctgcccacg acgtgatcag gtcgagggga gtcccgggag tgaagatggt ggccatcccg
     1141 gcgtcgagca gggcctgaaa atcggcctcg gggatcacgc ctccgccgaa cacgacgaca
     1201 ttcgtggcgt cgcgggcggc cagttcctcg accacctggg gaaaaagggt cttgtgcgca
     1261 ccggaaagcg acgacagacc gacggcatcc acgtcttcct gaaccgcggc ttcggcgatc
     1321 tgcgccggag tctggtgaag gccggaatag atgacctcga agccggcatc tcgcagtgcc
     1381 cgagcgatca ctttcgctcc gcgatcatgg ccatcgagac ccggtttggc gatgaggatg
     1441 cgtcgaggca ttccgggacg gagcgtagtt cggatcagcc cccttcaatc cggtgccatg
     1501 acgcccaatc atcgagatgg gtcagcttga actcggcgca gatgggctct tgaaagttgg
     1561 gctcttcgac acacgcagcc tgataatcag cgggaaacag gaggttggcg aacttccgga
     1621 acgaagcggt cagcgtctcg tcctcgagat atggcccggc gacgaagagg agccgcccgt
     1681 cttcgacgcc aaattgctgg gacacgacgg ccggcggctt ctggaccgcc tcgttgagga
     1741 cgttcgagta ggcgacatcg cacctgatcg ttgagggcgg gaggggaacg cattcgagga
     1801 tgtcggtcgt ggccacgagc gtctgctggt attggacctc gtcacgagcc cgttgttcgg
     1861 acggtccggt gcgcaagcgt tccggcgccc tgagcgcctg ggcgccatcg aaatcgcccc
     1921 cgtaccaggc ctcgaggtga gattcgatga gccggagatc ggactgcgtc atgaggtcca
     1981 tcccaccccg ctcgcctatc gccagggtgg cggtggcgag aactccgact atggccgcag
     2041 agacccaatg cgccgggccg ggtgaacggc ggggccgggc tggcggggcg atcagcccgt
     2101 caggctcgac gaggacgggg ctcacgccca ccgagcctag atggccagga gtcgcgtatg
     2161 ccgggaccga cctacccttg aggaatgagc gcttccaacc tgcacagact cgccgcagcc
     2221 ggtgtctcca tctggtccga ccagatctcc aagaccatgc tggattcggg ggaactcgcc
     2281 cggcgaatcg tcgaagacgc agtgacgggg gtcacgtcga atcccaccat cttcgccggc
     2341 gccatcgtgg ggtcttccga ctacgacgac ctgctccgag agctgaagtc cgccgacgtg
     2401 tccaccgagg agatcgccaa gactctcatg gccaccgacc tgaagcgagc ctgcgacgac
     2461 ttgatcggcg tcttcgagaa gaccgacggc actgatgggt ttgtctccgt cgaagtggcg
     2521 cccaccctcg ccggtgacac cgaggcgagc gtggccgagg cccgggactg ggtcaagcag
     2581 atcgaccgac ccaatttgct ggtgaagatc ccggccactc cggaagggat ccccgcgatc
     2641 cgcagccttc tcgctgaggg gatctcggtc aacatcaccc tcatcttctc tcttgagcgg
     2701 tacggggagg tcatggacgc atacctcggc gggttggagg agtacgtcgc atcaggggga
     2761 gacccgggtc gggtggcgtc ggtggcgtcg ttcttcgtgt ctcgcgtcga caccgaggtc
     2821 gacaagcgcc tggacaccat cgggacaccc gaggctctcg ccctgaaggg ggtcaccgcc
     2881 atcgccaatg cccgggtcgc ctacgggcgg ttcctcgagg cgtttggctc cgaccgatac
     2941 gcggcgctgt cgaaccaggg ggcacgagag cagaagccgc tctgggcttc gacctcgacc
     3001 aagaaccccg actacccgga tctgctgtat gtggagaacc tggtggcccc gcacacagtc
     3061 aacacgatgc cgctcgaaac aatcgacgcg tatcaggacc acggcaacgc gtcacccgag
     3121 gctttcaccg aagcggatat cgagcgggcc agtgccgacc tcgaccgtct gggtgaagtc
     3181 ggcgtcgact acgacgacgt cgtccagacc ctcgaagacg aaggcgtcga caagttcacc
     3241 aagagctggc tggagctgct cgaggacatc gagaaggcat agccgttccg ggaagaaatc
     3301 ttcgaaatct gcgcacgagc cccttgcttc tgtcctccct cgaacgtatg ttcggggtat
     3361 ggggcaggcg cttttctcga ccgatcacct cgaacagagg atgctcaacc ggcaggccga
     3421 aatcagccgc ctcatcgccg agcagctcgt cgacttggag gaaattgatt acagacaggt
     3481 ggcgactgcg gacggatgcc gcagcctttc ggaatggacc gcgtcccgac tcgatctgag
     3541 ccccgaatcg gccaagacca tggtccgaac catgcggaga acggttgatc gtcctgatct
     3601 gcgagaggca ttgatctccg gtgcgatcag tttcgaccgt ctcgaagctc tctcccggat
     3661 ccccgagcac gtcggtcacc tggagcattt ggatgtggcc ggagtgcgac gccaggcggc
     3721 gatgcgggct cgaatcagcg tggaggacga atatcggacc tcctccgacc aattcttcgt
     3781 catgcagccc tcgttggacg aatcctggtg gaagctgtgg ggaggtctcg acggagccac
     3841 cggtgctctc gtggacaaga ccctgtccgc agccgccgat gacctaccac tgctccctga
     3901 cgggactcgg ggagacagcg catggctgaa ggccatcgcc ctggccgacc tctgcctcag
     3961 cgacgatcca ccccccgccc agctcagcgt gttcatcgac gcctcccagg cgactccgag
     4021 caacggaaag gcgggcatcg aattggaggc cggggcccag gatcggccgc gcagccctcg
     4081 aagcaatcct ctgtgatgcg gtcacagaag tgacatgccg agccgaggac ggcatcccga
     4141 tgcgatatgg gagacgatgg cgcaccattc ctcccgccct gcggcgggcc atcatccacc
     4201 gcgacaacaa cacctgccgc gcagacggct gtatcagcac ccac
//
LOCUS       DASTXO010000547         2672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1617144, whole genome shotgun sequence.
ACCESSION   DASTXO010000547 DASTXO010000000
VERSION     DASTXO010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617144"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VFU96_11600"
     CDS             complement(<1..292)
                     /locus_tag="VFU96_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017300996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU5113953.1"
                     /translation="MPDKPNILVLWGDDIGWWNVSAYNHGSMGYRTPNIDRIGREGAM
                     FTDWYGQQSCTAGRAAFITGQSPIRTGLTKVGLPGASLGLQPEDPTIAELLKP"
     gene            complement(285..1232)
                     /locus_tag="VFU96_11605"
     CDS             complement(285..1232)
                     /locus_tag="VFU96_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019501465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEU5113954.1"
                     /translation="MRRIPGGRFRMGSEDFYPDERPVHEVAVDGFWMDEHPVTNAEFR
                     RFVKETGYVTVAERPLDPADYPDADPDLLVPGSLVFHKTPGPVDLNDYRNWWEYVPSA
                     HWRRPGGPGTTFHGKGDHPVVHIAWEDADAYARWIGKELPTEAEWEFAARGGLDGAVF
                     AWGDEHFPGGKAMANSWQGEFPWENLKIDGFEGTSPVGSFPANGHGLYDMTGNVWEWT
                     SDWYVARHPDPAEHACCVPTNPRVTSPDQSYNPGQPGEHIPRRVIKGGSHLCAPNYCL
                     RYRPAARQPQMIDTSMSHLGFRCIVRSLGPEGKEQTTNA"
     gene            complement(1435..2217)
                     /locus_tag="VFU96_11610"
     CDS             complement(1435..2217)
                     /locus_tag="VFU96_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEU5113955.1"
                     /translation="MSRPVRIAVQLQPQHGDYAGIRAAAVRAEEMGVDVVFNWDHFFP
                     LSGTADGKHFECWTMLASFAEVTERVEIGALVTANSYRNPDLLADMARTVDHISGGRM
                     ILGIGAGWFEHDYDEYGYEFGTPGTRLSDLRDALPRIENRWAQLNPPPIRDIPILIGG
                     GGERRTLRYAARHADIWHGWGRPDTVRHKCEVLDNWCAREGRDPAEIERSCGANPIKP
                     ETGDGYVEAGATLITLSFDGRESWDLAPLKEWLSWRDQRNRR"
     gene            complement(2228..>2672)
                     /locus_tag="VFU96_11615"
     CDS             complement(2228..>2672)
                     /locus_tag="VFU96_11615"
                     /inference="COORDINATES: protein motif:HMM:NF024221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEU5113956.1"
                     /translation="GSISKIYETFYEGIVFLALTATVAVFVGALLLYVFRIIKVTQRT
                     RSVIIIATVGIGLFYLATLLLSIFGVDVPLVTGVGTGALVFSIVVVIVAALNLLLDFQ
                     VIEHGIATGAPRAFSWFAAFGLMVTIIWLYIEILRLIAIVFARRG"
BASE COUNT          458 a          862 c          862 g          490 t
ORIGIN      
        1 ggggcttgag gagctcggcg atcgtcggat cctccggctg gagcccgagg gaggcgccgg
       61 gcagcccgac cttggtgaga ccggtgcgga taggcgactg accggtaatg aaggcggcgc
      121 ggccggcggt gcatgactgc tggccgtacc agtcggtgaa catcgccccc tcgcgcccga
      181 tccggtcgat gttcggcgtc cggtatccca tagacccgtg gttgtaggcg ctcacgttcc
      241 accagccgat gtcgtcaccc cacaggacca ggatgtttgg tttgtcaggc attggtcgtc
      301 tgttcctttc cttcaggccc cagcgatcgg acgatgcacc ggaagccgag atgcgacatc
      361 gaggtatcga tcatctgcgg ttgccgggct gccggtcggt agcgcaggca gtagttgggt
      421 gcgcacagat gtgagccgcc cttgatcaca cggcgtggga tgtgctctcc cggttgtccc
      481 gggttgtagc tctgatctgg tgacgtcacg cgggggttgg tggggacaca gcaggcgtgc
      541 tccgccgggt ccggatgtcg agccacgtac cagtcgctgg tccactccca gacattcccc
      601 gtcatgtcat agagcccgtg cccgttggcc ggaaaactgc ccaccggcga cgtcccctcg
      661 aagccgtcga tcttgaggtt ctcccagggg aactcccctt gccaactgtt tgccatcgcc
      721 ttcccgccgg ggaagtgctc gtccccccaa gcaaagaccg caccgtcgag cccgccacgt
      781 gcggcgaact cccattccgc ctcggtgggc agttccttgc cgatccagcg ggcgtatgcg
      841 tcggcgtcct cccaggcgat gtggacgacg ggatggtcgc ctttcccatg aaacgtcgtt
      901 cccggccctc ccggacgtcg ccagtgggcg ctcggcacgt actcccacca gttccgatag
      961 tcgttgagat cgaccgggcc cggggtcttg tggaacacga gagaaccggg caccaagagg
     1021 tcaggatcgg catccgggta atcggcgggg tcgagtggac gctcggccac ggtgacgtag
     1081 cccgtctcct tgacgaacct gcggaactcg gcgttggtca ccgggtgctc atccatccag
     1141 aatccatcta ccgcaacctc atggacgggc ctctcatcgg ggtagaaatc ctccgagccc
     1201 atgcgaaacc ggccgcccgg gatgcgccgc attccctccc ggtctcttcg ccccggggat
     1261 atctcgattc gtgcctcgct gaccatttgg gaggtcgccg ggcccggagc ttcgcctttc
     1321 ggactgcgat cacccatgcc tgtgtcgcgg cctcggggag gatctgacca atagtggcgc
     1381 gcccatggtc ggcgaaccta gcgcgtcaca ttggggccct cgacaccagt ccgactaccg
     1441 ccgattcctc tgatcgcgcc atgacagcca ctccttcagc ggggcgagat cccacgattc
     1501 ccggccgtcg aaggacaggg tgatgagtgt ggccccggct tccacgtaac cgtcaccggt
     1561 ctccggcttg atcgggttgg ccccacacga gcgctcgatc tccgccgggt cccgcccttc
     1621 gcgtgcgcac cagttgtcga gcacctcgca cttgtgccgg actgtgtcgg gccgccccca
     1681 cccgtgccag atgtcggcgt gacgggcggc atagcggagg gttcgtcgtt ccccgccacc
     1741 gccgatcagg atcgggatgt cccggatcgg tgggggattc agctgggccc agcggttctc
     1801 gatcctgggt agcgcatccc tcaggtcact gagccgggtg ccgggagtac cgaactcgta
     1861 gccgtattcg tcgtagtcgt gctcgaacca tccggcaccg atgccgagga tcatcctgcc
     1921 cccggatatg tgatcgactg tgcgtgccat atcggccagg aggtcggggt tgcggtaact
     1981 gttggctgtc accaaggccc cgatctcgac ccgctcggtg acctcggcga acgaggcgag
     2041 catcgtccag cattcgaaat gcttcccatc tgcggtgcca ctgagcggga agaagtggtc
     2101 ccagttgaag accacgtcga cgcccatctc ctcggctcgc accgcggcag cccgtattcc
     2161 ggcgtagtcg ccgtgctggg gctgtagctg gactgcgatt cgcaccggtc tgctcaccag
     2221 ccggaaccta accccttcgg gcgaagacga tcgcgatcag tcggaggatc tcgatgtaca
     2281 gccagatgat ggtgaccatc agcccgaagg cggcgaacca ggaaaacgcc ctcggggccc
     2341 cggtggcgat gccgtgctcg atcacctgga agtcgaggag gaggttgagt gcggcgacga
     2401 tcacgacgac gatgctgaac acgagggcgc cggtgccgac tcccgtcacc aggggcacgt
     2461 cgacaccgaa gatggagagg agcaaggtgg ccagatagaa caggccgatt ccgaccgtgg
     2521 cgatgatgat caccgaacgg gttcgctggg tgaccttgat gattcgaaag acgtagagga
     2581 ggagagctcc cacgaacact gccacagttg cggtcaaggc aaggaacacg atgccctcgt
     2641 agaaagtctc gtagatcttg gagatcgaac cg
//
LOCUS       DASTXO010000548         2409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_954510, whole genome shotgun sequence.
ACCESSION   DASTXO010000548 DASTXO010000000
VERSION     DASTXO010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954510"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VFU96_11620"
     CDS             <1..653
                     /locus_tag="VFU96_11620"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5113957.1"
                     /translation="ETAGHIAEAGGERLVVETDVTSEASVGLMVTTVKERLGRIDNLV
                     NNSGVAGPSGRLWEVDPDEWRSTFEVNVFGVFAVTRAVLPVMVGQGSGSVVIVGSISG
                     KRPLFGRSAYTSTKTALIGMTRTLATEAGPHGIRVNLVSPGFVAGPRIEWVMKAQADA
                     RGIDVDEVRAEFEGESPLGRLTDPSDVARTVAFLTSESSAGITGADLNVNSGVVMY"
     gene            667..1353
                     /locus_tag="VFU96_11625"
     CDS             667..1353
                     /locus_tag="VFU96_11625"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEU5113958.1"
                     /translation="MSRLIDLSQPIYEGMKVYPGHLKTVTFQHVTHEETAPRFTSDFS
                     FQTWGFMLNDNGPTHVDSFSHLDPDPTAPTIDQMDLGLFYGPAVCIDVSHIEPRTDIS
                     PGDLDQALKSAGLTIEPGDHCFFYTATYDRYHDQPEYLTSFAGLGESACDWILDKGIV
                     TFGVDSPTPDNPASTEYPVHLMCRHERKTHYENLVLTEVVGKRFTFVGFPLKVVGAHG
                     GPTRAVAIVD"
     gene            complement(1360..2112)
                     /locus_tag="VFU96_11630"
     CDS             complement(1360..2112)
                     /locus_tag="VFU96_11630"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5113959.1"
                     /translation="MARVAIDHLSKTFPPPAKGGDPVVAIGDISLEVDGNVFVSIVGP
                     SGCGKSTLLNILSGVETPTRGKVSVTEGDRPAHLGYVFQDARLLPWRTVMENMLYVRD
                     DGDAGAAKAARYLDMVGLSHTHEMFPGQLSGGMQQRVGIARAFSVEPDLLLMDEPFSH
                     LDAITARSLRDHLQELWSATRKTVLFVTHDVVEAVQLSDRIVVLAPGGRIFDDISIDL
                     PHPRRAADPKVATLQAEILGRFEDMQPTSAPA"
     gene            complement(2114..>2409)
                     /locus_tag="VFU96_11635"
     CDS             complement(2114..>2409)
                     /locus_tag="VFU96_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113960.1"
                     /translation="PAVAGYIFAGLRFAIIIGWNGILLSEWFSGQAGVGFRTRYWFDA
                     NRYRGFVGWVIVFIVFIVLVDRVILNRLQTRAFRWRDQKEVDFIEIQEQQVGL"
BASE COUNT          459 a          749 c          776 g          425 t
ORIGIN      
        1 aggagactgc cgggcacatc gccgaagccg gtggggagcg gctggtggtc gaaaccgacg
       61 tgacctctga ggcatcggtc ggcttgatgg tgaccaccgt caaggagcgt ctgggccgaa
      121 tcgacaatct cgtgaacaac agcggcgtgg ctgggcccag cgggcgtctc tgggaagtcg
      181 acccggatga gtggcgatcc acattcgagg tgaatgtctt cggcgtcttc gccgtgacca
      241 gggcggttct ccctgtgatg gtcggtcagg gctccggatc ggtggtcatc gtcggctcga
      301 tcagcgggaa acgaccgctg ttcgggcggt cggcgtacac gagcacgaag accgccctga
      361 tcggcatgac ccgcacgctc gccacggagg ccgggcctca cgggatcagg gtcaatctcg
      421 tctcgcccgg gttcgtcgcc gggccacgaa tcgaatgggt gatgaaggcg caggcagatg
      481 ctcgggggat cgacgtggac gaggtgcggg ccgagttcga aggagaatcg ccgctcggca
      541 ggttgaccga tccatcggac gtggcacgga cggtcgcctt tctcacctcg gagtcctccg
      601 ccgggatcac gggggcggat ctcaacgtca actccggcgt cgtgatgtat tgagcaaagg
      661 agaagcatga gccgactgat cgacctttcc cagcccatat acgaagggat gaaggtgtat
      721 ccggggcacc tgaaaacggt gaccttccag cacgtcaccc acgaggagac agcacccagg
      781 ttcacctctg acttctcctt ccagacctgg ggattcatgc tcaatgacaa cgggcccacc
      841 cacgtcgact ccttcagcca tctcgacccc gacccgaccg cgccgactat cgaccagatg
      901 gacctcggcc tgttctatgg gccggccgtc tgcatcgacg tctcccacat cgagcccaga
      961 accgacatct ctccgggaga cctggaccag gccctgaagt cggccgggct gaccatcgag
     1021 cccggtgacc attgtttctt ctacaccgcc acatacgacc ggtatcacga ccagcccgag
     1081 tacctgacca gcttcgcggg cctgggcgag tcggcctgcg actggatcct ggacaagggc
     1141 attgtcacct ttggcgtgga ttcaccgacg ccggacaacc cggcgtcgac cgagtacccg
     1201 gtgcatctca tgtgtcgcca tgagcggaag acccattacg agaatctggt cctcaccgaa
     1261 gtcgtgggca agcgcttcac cttcgtcggt ttcccgttga aggtggtcgg agctcacggt
     1321 ggcccgacgc gcgccgtggc catcgtcgac tgaacgagct caggccgggg cgctggttgg
     1381 ctgcatatcc tcgaatcgac ccaggatctc agcctgcaat gtggccacct tggggtcggc
     1441 ggcacggcgg ggatgaggga ggtcgatgct gatgtcgtcg aagatccgcc ccccgggggc
     1501 gaggacgact attcggtcgg agagctgaac cgcctccacg acgtcgtggg tcacgaagag
     1561 caccgtcttg cgggtggccg accacagctc ctggagatga tcgcgcagtg agcgggccgt
     1621 gatcgcgtcg agatggctga atggctcgtc catcagcaag aggtcgggct cgacggaaaa
     1681 ggcacgggcg atgccgactc tctgctgcat gccgccggac agctgcccgg ggaacatctc
     1741 atgggtgtgg ctcaggccga ccatgtcgag gtagcgggca gccttcgcgg ctccggcgtc
     1801 gccgtcatcg cggacgtaca acatgttttc catcaccgtc cgccagggaa ggagacgagc
     1861 gtcttggaag acatatccga ggtgggccgg ccggtcgccc tccgtcacgg acaccttgcc
     1921 cctcgtcggg gtctcgacgc cggagaggat gttgagcaat gtgctcttac cgcatcccga
     1981 agggccgacg atggagacga agacgttccc gtccacctcc agcgagatgt cgccgatggc
     2041 gaccacggga tcgccaccct tcgccggtgg ggggaaagtc ttgctgagat gatcgattgc
     2101 tacgcgtgcc atgtcataga cctacctgtt gctcctggat ctctatgaag tcgacctctt
     2161 tctgatctct ccatcggaac gcccgcgtct ggagccggtt caagatcacc cggtcgacga
     2221 ggacgatgaa gacgatgaag acgatcaccc atcccacgaa gccccggtaa cggttggcgt
     2281 cgaaccaata cctcgtgcga aatccgacac cggcctgacc gctgaaccac tccgagagga
     2341 ggatgccgtt ccatccgatg atgatggcga agcggaggcc ggcgaagatg taaccggcta
     2401 ccgccggga
//
LOCUS       DASTXO010000549         3156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_144178, whole genome shotgun sequence.
ACCESSION   DASTXO010000549 DASTXO010000000
VERSION     DASTXO010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144178"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VFU96_11640"
     CDS             complement(<1..245)
                     /locus_tag="VFU96_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010217140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HEU5113961.1"
                     /translation="MRIISGRAKGRRLIAPDTPATRPVTDRAREAVFSMIGGWVEEAN
                     VLDLYAGSGSFGLEALSRGAATATFVESGNRALEALR"
     gene            complement(245..2410)
                     /gene="recG"
                     /locus_tag="VFU96_11645"
     CDS             complement(245..2410)
                     /gene="recG"
                     /locus_tag="VFU96_11645"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU5113962.1"
                     /translation="MATLSKLAGVSVTEVRGIGKDTEKALAKGGIRSVADLLHHLPRT
                     YVDRSDRPRLDRVPLGTEVTVIGEVKSIATRRPRAKFTITEARITDETATLKVVWFNQ
                     PFRERQLSPGTEAAFAGTIESYRGSLQMANPVVAVLDSDRETLHTGRIVPIYPQVGKV
                     KPFELIDWIANALSRARPIPDPVPDVVLAANGLMSRDEAYAGIHFPDTLDQTPPARQR
                     LAYDELLRLEVALALRKRKQTDEATGFAHDPSGELAAAFLRGLPYELTGAQRRVIDEI
                     LADLRESHPMHRLLHGEVGSGKTVVAVVTLLNGVEGGWQGAIMAPTEVLAVQHYLGVT
                     GLLAQAGLSPEPFAAPSELGMESLFESDGPAVHIGILTGSTAAANYNPGISRADLLED
                     VAAGQVDILVGTHALIQEGVHFHKLGVAVIDEQHRFGVSQRVMLKEKADSIDPDILIM
                     TATPIPRTLSMTLYGDLDISGLDEMPPGRSPVKTRVLSRLEESAAWKKIEEEVAAGRQ
                     AFVVCPLVDDSPKVEAASAKAEHERLSALMPGLRVGLIHGQLRPADKEEAMSGFRAGE
                     TDVLVSTTVIEVGIDVPNATVMVIEDADRFGLSQLHQLRGRVGRGRHPGVCLLIADPS
                     TDDAKERLAAMQATNDGFELANEDLRIRGQGTVFGERQSGMADLQVADLFRDLELLVA
                     ARNDAFAIVDQDPRLSDHPDLADEVRALLGESVDWLFKS"
     gene            2554..2742
                     /gene="rpmB"
                     /locus_tag="VFU96_11650"
     CDS             2554..2742
                     /gene="rpmB"
                     /locus_tag="VFU96_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007456456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU5113963.1"
                     /translation="MSYRCEICGKEPSYGKTVSFSHKRNNRRWLPNIQKVRVRHGSNT
                     RRARVCTSCIRAGKIEKA"
     gene            2745..2960
                     /locus_tag="VFU96_11655"
     CDS             2745..2960
                     /locus_tag="VFU96_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEU5113964.1"
                     /translation="MPQGVVKTYDPVTGAGIVVRDDDRSEVFLRPGSLKGSIFRTLRQ
                     GQRLVFETVEEEGSHFVSSVRVGQEGY"
     gene            complement(2964..>3156)
                     /locus_tag="VFU96_11660"
     CDS             complement(2964..>3156)
                     /locus_tag="VFU96_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEU5113965.1"
                     /translation="AEMWLSKLDWPFDVIEGGFRDPAIDGDDGDSGDRAIALVFAVRP
                     TKVLAFGKGEPYSQTRYRF"
BASE COUNT          536 a         1065 c          997 g          558 t
ORIGIN      
        1 tgcctcagcg cctcgagggc gcgattgccg ctctcgacga aggtggccgt cgccgcgccc
       61 cgggagaggg cctcgagccc gaacgacccg gatccggcgt agaggtcgag aacgttcgcc
      121 tcctccaccc agcccccgat catcgagaac accgcctccc gtgcccggtc agtcaccggt
      181 ctcgtggcgg gggtgtcggg agcgatgagg cgcctcccct ttgccctgcc actgatgatc
      241 cgcatcagct cttgaacaac cagtcgacgg actcaccaag cagcgctcgc acctcgtcgg
      301 ccaggtcggg atgatcgctg agccgtggat cctggtcgac gatggcgaag gcgtcgttgc
      361 gagccgcgac caggagctcc agatcgcgga acaagtccgc gacttggagg tcggccatcc
      421 ccgactggcg ctcaccaaac acggtcccct ggcccctgat cctcagatcc tcgttggcca
      481 gctcgaaccc gtcattggtg gcttgcatcg ccgccagcct ctcttttgcg tcgtcggtcg
      541 acggatcggc gatgagcagg cacaccccgg gatgccggcc gcgacccacc cgtcctcgca
      601 actggtggag ctggctcaag ccgaaccggt ccgcgtcctc gatcaccatg acggtggcat
      661 tgggcacgtc gatcccgacc tcgatcacgg tcgtggaaac gagcacgtct gtctcgccgg
      721 cgcggaatcc ggacatcgcc tcctctttgt cagcaggacg gagctgaccg tggatcagcc
      781 caaccctgag accgggcatc aacgccgaaa gacgctcgtg ctcggccttg gccgatgcgg
      841 cttcgacctt ggggctgtca tcgaccaacg gacagaccac gaacgcctga cgcccggcgg
      901 ccacctcttc ctcgattttc ttccacgccg ctgactcctc gaggcggctg agaacacgtg
      961 tcttcaccgg gcttcgcccg ggcggcatct catcgagtcc cgagatgtcc agatcgccat
     1021 agagcgtcat cgacagcgtc ctcgggatgg gtgtggcggt catgatcagg atgtcggggt
     1081 cgatcgaatc cgctttctcc ttcagcatca cccgttgtga gacaccgaag cggtgctgct
     1141 cgtcgatcac agccactccc agcttgtgga agtgcacccc ctcctggatc agggcgtgag
     1201 tccccaccag gatgtccacc tgccctgcgg cgacatcctc gaggaggtcc gcccggctga
     1261 tgcctgggtt gtagttggca gcagcagtcg agccggtgag gatcccgata tgcacggcag
     1321 gcccgtccga ttcgaagaga ctctccatac ccagctcgct gggtgcggcg aagggctcag
     1381 gtgagagccc ggcctgggcg agcagaccgg tgactccgag gtagtgctgc acggccagga
     1441 cctcggtcgg ggccatgatc gcaccctgcc atcctccctc gacaccgttg agcagggtga
     1501 caaccgccac caccgtcttc ccggagccga cctcgccatg gagcaggcgg tgcatcgggt
     1561 gactctcccg aagatccgcc aggatctcat cgatgacccg gcgctgtgcc ccggtcaact
     1621 catagggcag cccgcgcagg aatgcggcgg caagttcccc acttggatca tgggcgaacc
     1681 cggtggcctc gtcggtctgc ttgcgcttcc gaagcgccaa ggcgacctcg agacggagga
     1741 gctcgtcgta ggccaggcgt tgccgtgcgg gaggggtctg gtcgagggtg tccgggaaat
     1801 ggatcccggc atatgcctcg tccctcgaca tcagaccgtt cgctgcgagc accacgtcgg
     1861 gcaccggatc ggggatgggt ctggcccggc tcagggcgtt ggcgatccag tcgatgagct
     1921 cgaacggctt caccttcccg acttgcgggt agatcgggac gatccgaccg gtgtgcagcg
     1981 tttccctgtc gctgtcgagg acagcaacca ccgggttggc catctgcaga ctcccacggt
     2041 aggactcgat cgtcccggca aacgccgcct cggtccccgg agagagctgt ctctcgcgaa
     2101 agggctggtt gaaccagacg accttgaggg tggccgtttc gtcggtgatg cgggcctcgg
     2161 tgatggtgaa cttcgccctc ggcctgcggg tcgcgatcga tttcacctca ccgatcacgg
     2221 tgacttcggt tccgaggggg acccggtcga ggcgagggcg atcggagcga tcgacatagg
     2281 tccgtggaag gtggtggagg agatcggcca cgctcctgat tccccccttg gcgagggcct
     2341 tctcggtgtc cttgccgatg ccacgcacct cggtcacgga cacaccggcc agcttgctca
     2401 acgtggccat cagcgttggc gaaaagggat cggcgccacc agcaaacggt acagccggac
     2461 tgtgacttgg cccggatcag ggctatcctt ccgcacggtc gaaacgggca gcccgcccgt
     2521 ctacccttcc cgactgttcc aggagtcttc gagatgtctt atcgctgtga gatctgcggc
     2581 aaggagccga gctacgggaa aaccgtgagc ttctcccaca agcggaacaa tcgccggtgg
     2641 ctgcccaaca tccagaaggt gcgggtccgc cacggctcga acacccgtcg cgcccgcgtt
     2701 tgcacctcgt gcatccgggc gggcaagatc gagaaggcct gaccatgcct cagggcgtgg
     2761 tcaagaccta cgaccctgtg accggcgccg gcatcgtcgt ccgcgacgac gaccggtccg
     2821 aggtgttcct ccgtcccggc tccctcaaag gatcgatctt ccgcaccttg cgacagggcc
     2881 agcgtctcgt tttcgagacg gttgaagagg aaggctcgca tttcgtgtcg tcggtccgcg
     2941 tgggccaaga gggctattag gcctcagaag cggtaccggg tctggctgta gggctccccc
     3001 ttgccgaagg ccagcacctt ggtgggcctc accgcgaaca ccagcgcaat agcgcggtcg
     3061 ccgctgtcgc cgtcgtcccc gtcgatcgcc gggtcacgga atcccccctc gatcacgtcg
     3121 aagggccaat cgagcttcga caaccacatc tccgca
//
LOCUS       DASTXO010000550         4026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1764518, whole genome shotgun sequence.
ACCESSION   DASTXO010000550 DASTXO010000000
VERSION     DASTXO010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1764518"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VFU96_11665"
     CDS             complement(<1..353)
                     /locus_tag="VFU96_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113966.1"
                     /translation="MEAARLNRARVRRATGKTLLEGPGLLTAALDAGVTPDVVFALPD
                     DEMTALLGEQQGFELTLVESSVLSRVSGTETPRGPVAVITVPEPSRPTGRDMIVAWGL
                     GDPGNVGTLIRTAAA"
     gene            complement(410..760)
                     /gene="rplT"
                     /locus_tag="VFU96_11670"
     CDS             complement(410..760)
                     /gene="rplT"
                     /locus_tag="VFU96_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU5113967.1"
                     /translation="MARVKRAVHAKKSHKKVLDRAKGYKGARSRRYKTANEQVMHAMQ
                     DAYRDRRARKGEFRRLWIARINAAARQNGTTYSQLIAGLKAAEIEVDRKMLAEMAVND
                     PSAFSQLVEASRKG"
     gene            complement(780..974)
                     /gene="rpmI"
                     /locus_tag="VFU96_11675"
     CDS             complement(780..974)
                     /gene="rpmI"
                     /locus_tag="VFU96_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005319593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU5113968.1"
                     /translation="MPKMKTHSGAKKRIKVTGSGKYMRGQSGRGHLRLAKGKSTYRRR
                     TGEVELAPGDAKVAKKLLGR"
     gene            complement(993..1523)
                     /gene="infC"
                     /locus_tag="VFU96_11680"
     CDS             complement(993..1523)
                     /gene="infC"
                     /locus_tag="VFU96_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013018294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU5113969.1"
                     /translation="MNGAIRAKEVLVIAPDGAQIGVKKLNEALWLAEQLDLDLVEVAP
                     NAKPPVCRLMDYGKYKYEQSVKAREARKKQTRTTIKELRMKVRIGEHDFDITRRKAQE
                     FLEEGDKVKVTIRFRGREQERPAFGKELLDRLAEELSDVAVTEQNAKLEGRSMTMVLA
                     PSRKTKASVEPAEDEQ"
     gene            complement(1784..2245)
                     /gene="ribH"
                     /locus_tag="VFU96_11685"
     CDS             complement(1784..2245)
                     /gene="ribH"
                     /locus_tag="VFU96_11685"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEU5113970.1"
                     /translation="MTRTIEGELDGTSLHVGVAVATWNRSVTDRLLEGAVTRLEALGA
                     EVTVIRVPGALELPIAALGLADAGCDAVVAIGTIVRGDTDHYEIVVRESTAGIARVSL
                     DTGIPVANAILAVNEFAHAVERAAAGPSNKGFEAAEAAVRTAVAMRSLDAG"
     gene            complement(2242..3456)
                     /locus_tag="VFU96_11690"
     CDS             complement(2242..3456)
                     /locus_tag="VFU96_11690"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018350069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEU5113971.1"
                     /translation="MSFARIEDVISAVAHGEMVIMVDDEDRENEGDLILAADAATPEQ
                     VGFMLRHTSGIICLPVIGERLDELDLPMMVARNTDVRRTAFTVSIDARDGTTTGISAS
                     DRWKTIRAVIDPDTKPHDLARPGHMYPLRYEPGGVLKRAGHTEAAVDLARLSGHYPAG
                     VLAEVMNDDGSVARLTELEQFAEEHGLLIGTIADLIAFRRREEKLVERVVEARIPTEH
                     GTFTAVGYRSLVDDRQHIALVMGDLGDGESVLTRVHSECLTGDVFGSLRCDCGDQLDQ
                     AMARVAGEGRGVVLYIRGHEGRGIGLLHKLAAYKLQDEGFDTVDANVNLGLPVDARDY
                     GVGAQILYDLGVRSMRIMTNNPTKRAGIEGYGLSILEQVPLFIDANDENRDYLRAKAT
                     RLGHQMDLGQGA"
     gene            complement(3453..4001)
                     /locus_tag="VFU96_11695"
     CDS             complement(3453..4001)
                     /locus_tag="VFU96_11695"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEU5113972.1"
                     /translation="METADGGMRLVLDTGPLPRMELGGSIAVNGVCLTGVETGDGRIA
                     VDIVTETISRSTLGDLRPGDPVNLERPMRGDGRFDGHIVQGHVDGVGVIVSVEPEGQG
                     KRIRLRLPNDLSRYVIEKGSITVDGVSLTVAAVEGDEVEIALIPHSLQVTTLGLRSAG
                     EKVNLEVDLLAKYVEKLLRAGS"
BASE COUNT          646 a         1363 c         1234 g          783 t
ORIGIN      
        1 aacgcagccg cggtccggat caacgtcccg acgttccccg ggtcaccgag cccccaggca
       61 acgatcatgt ccctgccggt cggtcgcgat ggctcgggca ccgtgatgac cgccacggga
      121 cctcgaggcg tttcggtgcc actcacccgg ctcaataccg acgattcgac gagggtcaac
      181 tcgaagccct gctgttcgcc gagcagcgcc gtcatttcgt cgtccggtag ggcgaacacg
      241 acatcggggg tcacgccggc atccagtgcg gcggtgagaa ggccggggcc ctcgagaagc
      301 gtctttccgg tggcccgtcg cacccgggca cgattgagcc gggccgcttc caccacccga
      361 gggttgcgat ggctgcggac cggctccact gacaggcggt ggtcgggcgt cagcccttgc
      421 gggaagcctc gaccaactgg gagaaggcgc tcgggtcgtt caccgccatc tcggcgagca
      481 tcttgcggtc gacctcgatc tcggcagctt tcagacctgc tatcagctgc gagtacgtgg
      541 tcccgttctg gcgtgcggcg gcattgatcc tggcgatcca gagacgccga aactcgccct
      601 tccgggcacg acggtcacgg taggcatcct gcatcgcgtg catgacctgc tcattggcgg
      661 tcttgtatcg ccgactgcgc gcccctttgt atcccttggc ccgatcgagg accttcttgt
      721 gtgacttctt ggcgtggacc gcccgcttca cacgtgccat gactgaacct cctgaatcct
      781 caacgaccga gcagcttctt ggcgaccttg gcgtcaccag gggcaagctc cacctcgccg
      841 gtgcgacggc gataggtgct cttccccttg gccagacgca gatgcccgcg cccggattga
      901 cccctcatgt acttgcccga accggtgacc ttgatccgct tcttggcgcc gctgtgtgtc
      961 ttcatcttgg gcacggctac ctcctgttcg tcctactgct catcttcggc cggctcaacg
     1021 gaggcctttg tcttccggga aggagccagg accatggtca tgctccgccc ttcgagtttg
     1081 gcgttctgct ccgtgacagc gacatcgctc aattcctccg ccagccggtc gagcagttcc
     1141 ttgccgaagg cgggacgctc ttgttcacgt ccgcgaaagc ggatcgtgac cttgaccttg
     1201 tccccttctt cgaggaattc ctgggccttg cgacgggtga tgtcgaagtc gtgctccccg
     1261 atccgcacct tcatccgaag ttccttgatg gtggtccggg tctgcttctt ccgtgcctca
     1321 cgcgccttga ccgactgctc gtacttgtac ttgccgtagt ccatcagccg gcaaaccggc
     1381 ggcttggcat tgggcgccac ttccacgaga tcgagatcca gctgctctgc cagccatagg
     1441 gcctcattca gcttcttgac cccgatctgg gccccatcgg gggcgatgac caagacttcc
     1501 ttggccctta tggcgccatt cacacggagc tcgttgatgc tcttacctcc tgttgatgtt
     1561 tggttgtctg caaaagaaaa aggcaacagg cgagagacct gctgccatga cctcggaccg
     1621 ggcccctata gggagttcgg tctggtgatc ggaccacggc tcgcctctcc tgagatctat
     1681 ttggatcccg acggcggccg ggtggggaag cagcgcttcc gctcttgcca tcgattgtcc
     1741 cgtcaaggct accagttggc tgcccgaaac ctgtagtcgc cgctcagccc gcatcgaggc
     1801 tccgcatcgc cacggcggtg cgcacggcgg cctctgccgc ctcgaagccc ttgttggaag
     1861 ggcccgccgc ggcacgttcc acggcatgtg cgaactcgtt gacggcgagg atggcgttgg
     1921 caaccgggat gccggtgtcg agcgagaccc gggcgatccc ggcggtcgat tcgcggacca
     1981 cgatctcgta gtggtcggta tctccgcgca cgatggtccc gatcgccacc acggcgtcac
     2041 aaccggcgtc tgcgagcccg agcgcagcga tcggcaactc gagggccccg ggcacgcgaa
     2101 tcaccgtcac ttcggctcca agggcctcga gacgggtcac cgccccctcg aggaggcgat
     2161 cggtcaccga ccggttccag gtggccaccg cgacaccgac gtggaggctc gtgccgtcga
     2221 gttctccttc gatggttcgg gtcatgcccc ttggcccaga tccatttggt gtcccagacg
     2281 ggtggccttg gcccgcagat aatccctgtt ctcgtcgttg gcgtcgatga acaggggaac
     2341 ctgctccagg atcgacagac cgtatccctc gatcccggcg cgcttggtgg gattgttggt
     2401 catgatccgc atcgaccgca cgccgaggtc gtagaggatt tgggcgccca ctccgtagtc
     2461 gcgggcatcg accgggagcc ccaggttcac attggcgtcc acggtgtcga atccctcgtc
     2521 ctggagcttg tacgccgcca gcttgtggag caacccgatc ccccggccct cgtggccccg
     2581 gatatagaga acgacgccgc gtccctcgcc ggcaacacgc gccatcgcct gatccagctg
     2641 gtcgccacag tcacagcgca ggctgccgaa gacatctccg gtgaggcact ctgaatggac
     2701 cctggtgaga accgactcac cgtctcccag gtcccccatc accagggcga tgtgctgcct
     2761 gtcgtccacg agcgatcgat accccaccgc ggtgaaggtc ccgtgctcgg tggggatgcg
     2821 ggcttcgacg acccgttcga ccagcttctc ctcacggcgg cggaaggcaa tcagatcggc
     2881 gatggtcccg atgaggagtc cgtgctcttc ggcgaattgc tccaactcgg tcaggcgggc
     2941 caccgatccg tcgtcgttca tcacctcggc gagcacacct gccgggtagt gccccgacag
     3001 ccgggccagg tcgaccgcgg cctcggtatg tccggctctc ttgaggaccc cacctggctc
     3061 gtagcgcagc gggtacatgt ggccggggcg ggcgagatcg tggggcttgg tgtcgggatc
     3121 gatcacggcc cgaatggtct tccagcgatc ggaggccgag atgccggtgg tggtcccgtc
     3181 ccgtgcgtct atggacacgg tgaaggcggt gcgccggacg tcggtgttgc gggccaccat
     3241 catcggcagg tcgagctcgt cgagtctctc accgatgacc ggaagacaga tgatgccgct
     3301 ggtgtgacgc agcatgaaac ccacctgctc cggagtcgcc gcgtcggcgg cgaggatgag
     3361 atcgccctcg ttctcgcgat cctcgtcgtc gaccatgatg accatctcgc cgtgtgccac
     3421 ggccgagatg acatcttcga tccgggcaaa gctcatgatc ccgctctcag cagcttttcg
     3481 acgtacttag ccagcaaatc gacctcaagg ttgaccttct cccccgcgga ccgcaaaccc
     3541 aatgtggtca cctgcaacga gtggggtatc aaggcgatct cgacttcgtc accctccacc
     3601 gccgccactg tgaggctgac cccgtcgacg gtgatcgagc ccttctcgat gacgtaacgg
     3661 ctgagatcat tggggagccg gagccgaatc cgcttgccct gaccctcggg ctcgaccgag
     3721 acgatgactc caacgccatc gacgtggcct tgcacgatgt gcccgtcgaa cctgccgtcc
     3781 ccccgcatcg gtcgctccag gttgaccggg tcgcccggcc gcaggtcacc gagggtggaa
     3841 cggctgatgg tctcggtgac tatgtcgacc gcgatcctcc cgtcgccggt ctcgacaccg
     3901 gtcagacata ccccgttgac cgcgatcgaa ccacctagct ccatccgggg aaggggcccg
     3961 gtgtccagga cgaggcgcat accaccgtca gctgtctcca ctgagaggac agagccccgg
     4021 tgttcg
//
LOCUS       DASTXO010000551         7897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1396563, whole genome shotgun sequence.
ACCESSION   DASTXO010000551 DASTXO010000000
VERSION     DASTXO010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7897
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396563"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VFU96_11700"
     CDS             complement(<1..289)
                     /locus_tag="VFU96_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5113973.1"
                     /translation="MKAFRASIFILALVLVACGGDGGGDTTVAETTTTAAEADGGAGT
                     VDFEDMPQECIDALVGYLRAIEPAVEDVDFDATTGEDLEALGTELDSLSEDY"
     gene            complement(348..2231)
                     /locus_tag="VFU96_11705"
     CDS             complement(348..2231)
                     /locus_tag="VFU96_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEU5113974.1"
                     /translation="MALPRKRGKRYLLVMSVGALGIVYGDIGTSPLYAFRESFHAAEG
                     LPVSRESVLGVLSLMFWALVLVVSVKYLMFVMRADNHGEGGILALTALIVPRRKTPTV
                     GLRWALILLGLFGTALLYGDGIITPAISVLSAVEGLEIVEPGLESYVVPIAVVILIAL
                     FSIQRAGTAVVGSIFGPVMIVWFTVLAVLGGFQVIQDPGVLAGVNPAYGAQLIVESPR
                     HAFLALGAIFLVVTGSEALYADMGHFGKRPIRLGWYIVVFPALLLNYFGQGAMLIADP
                     GTIDNPFFRMPPEWAVLPLVILATMATVIASQALISGAYSLTMQAVQLGYLPRFDIDH
                     TSPREVGQIYIATVNWGLMVACVGLVLSFRSSGSLAAAYGVAVTTTMVITTILLYFVM
                     RERWKWSAAVAGSLTALFLIVDLTFFSANIVKVPAGGWFPLVVGAVIFTIMTTWKTGR
                     RRLTAALKRGELPIERFIGSITLHPQLRVPGTAVYLFPDPGITPPALLANLRHNEVLH
                     ETVVLVAVQTSSVPRVPQVRRATVHDLGEGFVQVVLHFGFMDEPDVPKALANIVDPEF
                     GFDPDDSTFFIGRETILNSGKGIGRARAALYALMHRNASSPVQFFTLPPGQVIEIGKQ
                     VAL"
     gene            2345..2833
                     /locus_tag="VFU96_11710"
     CDS             2345..2833
                     /locus_tag="VFU96_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEU5113975.1"
                     /translation="MIDVLVTMIVEIPKGSRNKYETDHESGEIFLDRMLFTATRYPAD
                     YGFIPETHADDGDPLDILALVSEPTFPGCRIRVRPLGLFLMEDQGHADHKVLAVPDGD
                     PLWRDATDLDDVPEHLLRELEHFFAVYKELEDKKTATLGWRPAAEAFETIDRALEAGR
                     AR"
     gene            complement(2826..4178)
                     /gene="dnaB"
                     /locus_tag="VFU96_11715"
     CDS             complement(2826..4178)
                     /gene="dnaB"
                     /locus_tag="VFU96_11715"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU5113976.1"
                     /translation="MSGITDQGQMRPGFRNPPHSREAEESVIGAVLLSEEAVNEVMDR
                     LHPPDFYVPAHQAIFEAMRELFDTNQAIDAVTVSEALRRRGELEKVGGVAYLTRLVDI
                     VPSTSNILYYSGIVEEHAKRRELIRAGASVTDVAFDIDEEIARVLDRAEQAVLSVAER
                     RSAQTLLEVGPLFSDVLEHIELLEQRGSELTGLATGYVDLDKKLAGLQASNLVVIAAR
                     PAMGKSSLALGIAVNAASQNEPVAIFSLEMSKEEIVQRILSSVGRVDSMKLRSGQLGP
                     LWQRVVDAAGKMYQAPIYIDDSPVVTVTDIRAKCRRLKRKKGLSLVVIDYLQLMEASS
                     RENRQQEISEITRNLKNLARELEVPVIAVSQLNRSLEAREDKRPRLSDLRESGSIEQD
                     ADVVMFIYRHEYYHPEDQERKGIAEVIVAKHRAGSTGPVEVTFQPEFTRFANLGRDVT
                     "
     gene            complement(4432..4881)
                     /gene="rplI"
                     /locus_tag="VFU96_11720"
     CDS             complement(4432..4881)
                     /gene="rplI"
                     /locus_tag="VFU96_11720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU5113977.1"
                     /translation="MRLILISDVQELGKRGDVVDVAEGYARNYLLPRKKAVRANEGAL
                     AQAEAIREARVEAERKAKDEAERLATQLVGSRVVIAAQAGDEGQLYGSISPADVVEGV
                     RRFTGIELDRATLEIPQPIKAIGLHEINIKLHPEVEFPLTLDVIPAT"
     gene            complement(4878..5102)
                     /gene="rpsR"
                     /locus_tag="VFU96_11725"
     CDS             complement(4878..5102)
                     /gene="rpsR"
                     /locus_tag="VFU96_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU5113978.1"
                     /translation="MEMRRGRPKPCAFCKQQVDFIDWKDVSVLRKYMSDRGKIRGRRV
                     NGNCPTHQREVANAIKNAREMALLPYLNTR"
     gene            complement(5168..5626)
                     /gene="ssb"
                     /locus_tag="VFU96_11730"
     CDS             complement(5168..5626)
                     /gene="ssb"
                     /locus_tag="VFU96_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU5113979.1"
                     /translation="MSNNVTLIGNLVDDPELRFTPSGVAMAKIRLAVNRRWRGNDGEW
                     QEDTSFFTGTVWREQAESAAESLQKGARVIVTGRLEQRSWETEQGEKRSVVEVQIDEI
                     GPSLRWATASVNKTQRSGGDWGGGGGGGQGADVPPAPVARESYGPDEAPF"
     gene            complement(5689..5976)
                     /gene="rpsF"
                     /locus_tag="VFU96_11735"
     CDS             complement(5689..5976)
                     /gene="rpsF"
                     /locus_tag="VFU96_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009145514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU5113980.1"
                     /translation="MRDYEVMTIHRPDLAEDEVETKITDLESHLSGHGATVDGRDLWG
                     KRRFAYEIDHLSEGYYSVVTFQAEAAAVDSLDRMLSLADEVVRHKIVRKGA"
     gene            complement(6090..6413)
                     /locus_tag="VFU96_11740"
     CDS             complement(6090..6413)
                     /locus_tag="VFU96_11740"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEU5113981.1"
                     /translation="MWVSDSQEERSKGLSMLEELPEGIDGMMFIFDPPGVPTFTMKDT
                     VIPLDLWHFDPAGALISMVAMEPCTEEPCTLYPTSFPVGWSLETPSGDFTFEDGAMLT
                     TSGSG"
     gene            complement(6554..7402)
                     /gene="panB"
                     /locus_tag="VFU96_11745"
     CDS             complement(6554..7402)
                     /gene="panB"
                     /locus_tag="VFU96_11745"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005036056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEU5113982.1"
                     /translation="MQSKVTVPAIKARKGGPKISMVTAYDYPGAVIADRAGVDIVLVG
                     DSAANVVHGMSTTLEFGMEETIMHARAVKRAEPKALFVVDMPWLSFHVSVEDTVRNAG
                     RIVQEGGAEAVKLEGGRKRLPMIQAILDAEIPVMGHLGLTPQSVHAMGGFKVQGKMVD
                     AAQELIEDAIAIAEIGCFSVVLEGVPDILGELITKEVDIPTIGIGAGPATDGQVLVFH
                     DVLGLGSGSYPKFVRPYASLADQAVAALERYVADVTSGDFPGEDESYHVPDEIAAEML
                     RGRKSG"
     gene            7507..>7897
                     /locus_tag="VFU96_11750"
     CDS             7507..>7897
                     /locus_tag="VFU96_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEU5113983.1"
                     /translation="MAKVSRWRLFWTVVSIGAAVYIVLNNPVNLGLDLRGGTQIVLEA
                     QDTADVTVDSDVTARTLEVLRRRVDAFGVAEPTLQVSGDRRIIIEMPGVEDPDEALEV
                     IGRTAQLTFHPVLEAVPTGTEPTTESDL"
BASE COUNT         1449 a         2528 c         2503 g         1417 t
ORIGIN      
        1 tgtaatcctc ggatagcgaa tccagctcgg tgccaagggc ttcgaggtcc tcgcctgtgg
       61 tcgcgtcgaa gtcgacgtcc tccacagccg gctcgatcgc ccgcaggtat ccgaccaagg
      121 cgtcgatgca ctcctgaggc atgtcctcga agtcgaccgt tcctgcacca ccgtccgcct
      181 ccgcggctgt ggtcgtggtc tcggccacgg tcgtgtcccc tccaccgtca ccgccacagg
      241 ccaccagcac cagggccagg atgaagatcg atgctctgaa tgccttcatg gcgccccctc
      301 gactcaggag tccgaatctc ggacgttgcc agcgtaaatg gacaaggtca caacgccact
      361 tgcttcccga tctcgatgac ctgaccggga ggaagggtga agaactgaac cgggctcgac
      421 gcgttccggt gcatcaatgc gtagagagcc gctcgggccc gtccgatgcc cttgccactg
      481 ttgaggatgg tctctcgccc gatgaagaag gtcgagtcgt ccgggtcgaa cccgaactcc
      541 gggtccacga tgttggccaa cgccttgggg acgtcgggct cgtccatgaa cccgaaatgc
      601 agcacaacct ggacgaagcc ctctccgagg tcgtgaaccg tcgctcggcg cacctgcggc
      661 acacgaggga ccgaagacgt ttgcactgcc accaggacca cggtctcgtg gaggacttcg
      721 ttgtggcgaa ggttggcgag gagagccggg ggggtgatgc cggggtcggg gaacagatag
      781 acggcggtgc ccgggacccg gagctgggga tggagggtta tcgagccgat gaaacgctcg
      841 atcgggagct cgcctcgctt cagggcagcg gtgagtcgtc gtcggcccgt cttccaggtg
      901 gtcatgatgg tgaagatcac ggcaccaacc accagtggga accagccgcc cgcagggacc
      961 ttcacgatgt tggccgagaa gaacgtcagg tcgactatga gaaagagcgc cgtgagggat
     1021 ccggcgactg cggcggacca tttccatctc tcccgcatca cgaagtacag gaggatcgtc
     1081 gtgatgacca tcgtcgtcgt cacggccacg ccgtatgcag cggcgaggct tcccgagctg
     1141 cgaaacgaga gcaccaggcc aacgcaggcc accatcaacc cccagttcac ggtggcgatg
     1201 tagatctggc cgacttccct cggtgaggtg tgatcgatgt cgaagcgggg cagataaccc
     1261 agctggaccg cctgcatggt gagggagtag gcacccgaga tgagggcctg ggaagcgatg
     1321 accgtggcca tggtcgcgag gatcaccagc ggcaggaccg cccactccgg cggcatccgg
     1381 aagaaggggt tgtcgatggt tcccgggtcg gcgatgagca ttgcgccctg gccgaagtaa
     1441 ttgaggagga gtgcggggaa cacgacgatg taccagccga gtcgaatcgg gcgcttgccg
     1501 aaatgtccca tgtccgcgta tagagcctca ctacccgtga ccacgagaaa aatcgcgccc
     1561 agcgcgagaa aagcatgtcg tgggctctcg acgatgagct gcgcgccgta cgccggattc
     1621 accccggcga gcacaccggg gtcctggatc acctgaaagc cgccgagaac ggcgaggacg
     1681 gtgaaccaga cgatcatcac cggtccaaag atcgagccga cgaccgccgt gccggcacgc
     1741 tggatgctga acagcgcgat caggatgacc accgcgatcg ggacgacata agactcgagg
     1801 ccgggttcca cgatctcgag tccttcgacc gcagacaaca ccgaaatggc cggggtgatg
     1861 atcccgtctc cgtagaggag ggcagtcccg aagagcccca gaagtatcaa ggcccaccgc
     1921 agcccgaccg tgggtgtctt ccgacgaggg acgatcaggg cggtgagggc caggatgcca
     1981 ccctccccgt ggttgtcagc ccgcatgacg aacatcaggt acttgaccga gacaaccaag
     2041 accagggccc agaacatgag cgaaagcacg ccgaggacgc tttctcggct cactggcaag
     2101 ccctcggcag catggaaaga ttcccggaag gcgtagaggg ggctggtccc gatgtcgccg
     2161 tagacgatgc cgagggcgcc taccgacatg acgagcagat agcgcttgcc gcgctttcta
     2221 gggagtgcca tcgagaggtt tatcccgagg gttgcgttgg gttggaggac gtagggagac
     2281 cgacccgggc tgtggcatcg ccacccatgt gaggcacaac cgggcgaggg tagcgatctg
     2341 tagggtgatt gatgtgctgg tgacgatgat cgtggagatc cccaagggaa gccggaacaa
     2401 gtacgagacc gaccacgaaa gtggggagat cttcctcgat cggatgctgt tcaccgccac
     2461 ccgatacccg gccgattacg gcttcatccc cgagacccac gccgatgacg gcgacccgct
     2521 cgacatcctc gccctcgtgt ccgagccgac attcccgggt tgtcggatca gggtgcgccc
     2581 ccttgggctg ttcctgatgg aagaccaggg tcacgccgac cacaaggtgc tggccgtgcc
     2641 cgacggcgac ccgctgtggc gagacgccac cgatctcgac gacgtccccg agcacctcct
     2701 gcgcgaactg gagcacttct tcgccgtgta caaggagctg gaagacaaga agacggcgac
     2761 tctggggtgg cgcccggcgg ccgaggcgtt cgagaccata gaccgggccc tcgaggccgg
     2821 ccgggctagg tgacgtcccg acccaggttg gcgaaccggg tgaattcggg ctgaaaagtc
     2881 acttcgaccg ggcccgtgga cccggcgcgg tgtttggcga cgatgacctc ggcgatgccc
     2941 ttccgctctt gatcctcggg gtggtagtac tcgtggcggt agatgaacat gacgacgtcg
     3001 gcgtcctgct cgatcgagcc gctttcccgt agatcggaga gacggggccg cttgtcctcc
     3061 ctggcctcca acgaccggtt tagctgggag acggcgatca cggggacttc cagctctcgt
     3121 gccaggttct tcagattccg ggtgatctcc gagatctctt gttgacggtt ctcccgactc
     3181 gacgcctcca tcagctgcag gtagtcgatg accaccagcg aaagcccttt cttccgcttc
     3241 agtctccggc acttcgccct gatgtcggtg accgtgacca ccggggagtc gtcgatgtag
     3301 atgggggcct gatacatctt cccggcggcg tcgacaaccc tctgccataa gggtcccagt
     3361 tgacccgagc ggagcttcat cgagtcgacc cggcccaccg aggagaggat gcgctggacg
     3421 atctcctcct tcgacatctc cagggagaag atcgcaaccg gctcgttctg gctggccgcg
     3481 ttgacggcga tgccaagtgc gagggagctt ttgcccatgg caggtcttgc agcgatcact
     3541 accaggttgg aggcctggag gccggccagc ttcttgtcga gatcgacata gccggtcgcc
     3601 aatccagtga gctccgagcc acgttgctcg agcaactcga tgtgctccag cacatcgctg
     3661 aacaagggac cgacttcgag gagggtctgc gcactgcggc gctcggccac cgagaggacc
     3721 gcctgctcgg ctcgatcgag gacgcgggcg atctcttcgt cgatgtcgaa ggcgacatcg
     3781 gtcaccgaag caccggcccg gatcagctcc cggcgtttgg catgctcctc gacgatcccc
     3841 gagtagtaga ggatgttgga ggtcgagggg acgatgtcga cgagtcgggt cagataggcg
     3901 acacccccga ccttctccag ctcccctcgt cgccgcagag cctcggagac ggttacggcg
     3961 tcgatcgcct gattggtgtc gaacagctca cgcatcgcct cgaagatcgc ttgatgcgcg
     4021 ggaacgtaga agtcgggtgg gtgaaggcgg tccatcacct cgttgaccgc ctcttcggag
     4081 aggagcaccg cccctatgac cgactcttcc gcctccctgg aatggggtgg gttccggaag
     4141 cccggtctca tctgcccctg atccgtgatt ccgctcatct ctccctcatc caacaatgcg
     4201 cgagtgacag atttcgcgga cctggtgggg ccctgacacg caagaccccg ccggagggcg
     4261 gggaagccga aactgccagc ggggaaggtg gctgaagata gggtcggccc agccggcgtc
     4321 acaagccaga aggctcatcg aatttcgcgc tgccctcttt tgttttcgcg gtggaaagga
     4381 ttgccaggaa agtcgcgaaa gttgctactg acccgctccg cccacaggtg atcaggtggc
     4441 cgggatcaca tccagggtga gagggaactc gacctcgggg tggagtttga tgttgatctc
     4501 atgcaggccg atcgccttga tcggctgggg gatctccaag gtggcgcggt ccagctcgat
     4561 cccggtgaag cgacgcacac cctcgaccac gtcggccggt gagatcgagc cgtagagctg
     4621 gccctcgtcc cccgcctgcg ccgcgatgac gacgcggctt ccgacgagct gggtggccaa
     4681 ccgttccgcc tcgtccttgg ccttgcgctc ggcttcgacc cgggcttcgc ggatggcctc
     4741 ggcctgagcc agggccccct cgtttgccct gaccgccttc tttcgtggca gcagataatt
     4801 acgggcatag ccctcggcga catcgaccac atcgcctctc ttgcccagtt cctggacgtc
     4861 ggagatgagg atgagcctca tctcgtgttc agatagggca ggagagccat ctcacgggcg
     4921 ttcttgatgg cgttggccac ctcgcgctga tgcgtcgggc agttcccgtt cacccggcgc
     4981 ccccggatct tgccccggtc ggacatgtac ttgcgcagca cggagacgtc cttccagtcg
     5041 atgaaatcga cctgctgttt gcagaaggca cagggcttgg ggcgtccccg tctcatctcc
     5101 atgccccgac gccgcttggg gcgcgggttg ttcgtgctct ttctcgccat cgttcaatgc
     5161 tcctggatca gaagggcgcc tcgtcgggcc cgtaggactc tctggccacc ggtgccgggg
     5221 gcacgtcggc gccctgtcct ccgccaccgc caccgcccca gtcgccaccg gagcgctggg
     5281 tcttgttcac cgaagccgtc gcccagcgca gcgagggccc gatctcatcg atctgcacct
     5341 cgaccacaga gcgcttctcg ccttgctcgg tctcccagct tcgctgctcg agacgaccgg
     5401 tcacgatgac tcgcgcccct ttctgcagcg actcggcggc gctctccgcc tgctcacgcc
     5461 aaacggtgcc ggtgaagaag ctggtgtcct cctgccactc gccgtcgttg cctcgccagc
     5521 gacggttcac ggcgagccgg atcttggcca tggccacccc ggacggggtg aagcgaagct
     5581 cggggtcgtc gaccaggttc ccgatcaatg tgacgttgtt cgacatgtga aatctcctct
     5641 actcatgtcc ctgggtcgat catgccctga ccctgggact gattccagtc atgcgccctt
     5701 gcgcacgatc ttgtgtcgaa ccacttcgtc ggccagcgac agcatccgat ccagtgagtc
     5761 gacggcagcc gcctctgcct ggaacgtgac caccgagtaa tagccctcgg acagatggtc
     5821 gatctcgtag gcaaagcggc gcttccccca cagatcccgc ccatcgactg tcgcaccgtg
     5881 gccggacagg tgagattcga gatcggtgat cttggtctcc acctcgtcct cggccagatc
     5941 gggccgatgg atcgtcatta cctcatagtc gcgcatgtga cctcctgtgg acatccgtgt
     6001 cggaaggccc caccaacacg gtggagcagg ggtttcacgc aaccgaacgg cgcgagacgt
     6061 cggtgcggat acggaattgt agggctaaat caaccggaac ccgaagtcgt gagcatcgcc
     6121 ccatcctcga aggtgaagtc tcccgatggt gtctcgaggg accagccaac cgggaacgat
     6181 gtcgggtaga gagtgcacgg ctcctcggtg cagggctcca tggccaccat ggagatcagc
     6241 gctccggccg ggtcgaagtg ccagaggtcg agcgggatca ccgtgtcctt catcgtgaat
     6301 gtgggaacac ccgggggatc gaatatgaac atcatgccgt cgatgccctc gggcaactcc
     6361 tcgagcatgc tcagaccctt gctccgttcc tcctggctgt cggaaaccca cacgttcagc
     6421 tcggtgtcac cgacagtgat ctgctcgacc gggaagaggt cttcgctcgc cgtgccgggg
     6481 acggatgctt cgacgggaac cgtggtgccg gtggaagccc cgccgcaggc cgcgacgagg
     6541 atgagggcgg cgactaaccg gatttccggc ccctcagcat ctccgcggcg atctcgtccg
     6601 gcacgtgata ggactcgtcc tcgccaggga aatcacccga ggtcacgtcg gcgacgtacc
     6661 tttccagggc ggcgacggct tggtcggcca ggctggcata ggggcggacg aacttgggat
     6721 agctgcccga tccgagaccc agcacgtcgt ggaagacgag gacttggccg tcggtagctg
     6781 gaccggcgcc gatgccgatg gtgggtatgt cgacctcctt ggtgatgagc tcgccgagaa
     6841 tatccggcac accctccagg acgacgctga aacagccaat ttctgcgatg gcgatcgcgt
     6901 cctcgatcag ctcctgggcg gcatcgacca tcttgccctg gaccttgaag ccacccatcg
     6961 catggaccga ttgcggagtg agcccgagat gtcccatcac cgggatctcg gcgtcgagga
     7021 tcgcctggat catcggcagc cgcttccgac ccccttccag cttgaccgcc tctgcgccgc
     7081 cctcctggac gatgcgtccg gcgttgcgaa ccgtgtcctc gacggacacg tgaaagctca
     7141 gccacggcat gtcgaccacg aacaacgcct tcggctctgc ccgcttcacc gcccgggcat
     7201 gcatgatcgt ctcctccatc ccgaactcga gggtggtgga catcccgtgg accacattgg
     7261 cggccgaatc tccgaccaag acgatgtcga cgcctgcccg atcggcgatc accgcgccgg
     7321 gatagtcata ggcggtgacc atcgagatct tgggtccgcc ctttctggcc ttgatcgcgg
     7381 gaacggtgac cttggattgc atgggggaag gataccggga cggctgcatt cgcatttccc
     7441 gatctcacat gactggcacg tgccgatacc atgcgccctg ccagccgaac atcccaagga
     7501 tccttcatgg ccaaggtgtc ccggtggcgt cttttttgga ccgtggtctc gataggcgct
     7561 gccgtttaca tcgttctcaa caacccggtg aatctcggct tggatctgcg cggcggcacc
     7621 cagatcgtcc tcgaggccca ggacaccgcc gacgtcaccg tcgacagtga cgtgaccgct
     7681 cggacactcg aggtcttgcg tcgtcgcgtc gacgcattcg gggtcgccga gcccacgctg
     7741 caggtgtcgg gtgaccgccg gatcatcatc gagatgcccg gggtggagga tcccgacgaa
     7801 gccctagagg tcatcgggcg aaccgcccag ctcaccttcc acccggtgct cgaagcggtg
     7861 ccaaccggca ccgagccgac gaccgagagc gacctcg
//
LOCUS       DASTXO010000552         2355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1323130, whole genome shotgun sequence.
ACCESSION   DASTXO010000552 DASTXO010000000
VERSION     DASTXO010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1323130"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VFU96_11755"
     CDS             complement(<1..702)
                     /locus_tag="VFU96_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014516159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5113984.1"
                     /translation="MAETLVEIRDLVKEFPVRGGILQRQVATVRAVAGVDLDIVNGET
                     LGLVGESGCGKTTLGRLLTRLLEPTSGTIQFDGVDITHLSGNDLKPFRRRVQIIFQDP
                     YGSLDPRSQVGPAIAEGLRLHGIGDKDEQQERVKEMLELVGLRGSHASRYPHEFSGGQ
                     RQRIGIARALILKPDLVVADEPVSALDVSVQAQVLNLLTELQEELNLTLLFVAHNLAV
                     VEHISDRVAVMYLGRV"
     gene            complement(695..1708)
                     /locus_tag="VFU96_11760"
     CDS             complement(695..1708)
                     /locus_tag="VFU96_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5113985.1"
                     /translation="MPAGEVLLDVRGLKTHFDTRQGVVKAVDGADFHVKRGEVLGLVG
                     ESGCGKSVTSLSIMGLIPHPGKVVAGEILFGGKDIAKMKADDVRALRGEHISMIFQQP
                     TSALNPVYRAGAQIKEVFELHRDWSAEVEEEKVIEVLTKVGIPDPKRRADAYPHELSG
                     GMAQRIMIAMALACEPELLIADEPTTALDVTIQAQILDLIRDLQKTSNTSVILITHDL
                     GIVAEMADRVSVMYAGEIVEETDVRSIFRNPLHPYTKGLIGSIPVLGSLREQLDVIPG
                     VVPSMIDLPKGCRFAPRCIPRVENSLQICTEIEPDLREVEDGHKVRCWLYYGPEKVED
                     PDG"
     gene            complement(1762..>2355)
                     /locus_tag="VFU96_11765"
     CDS             complement(1762..>2355)
                     /locus_tag="VFU96_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5113986.1"
                     /translation="LGFTTVLITIVIGTVIGAIAGYAGGWSDNVMMRIMDVVLGFPSL
                     ILAIAIVFVLGPGLRNTLLAIAIVTIPQYARVMRASVLSIKEVDYVSASRALGASRWK
                     VLVTRVVPNALTPLVVLGTLGIATVILEAAALGFLGLGAVPPTPEWGSMLAEERDRIF
                     TAPHLMIFPGLAIMITVLGFNLMGDGLRDALDPTLNK"
BASE COUNT          442 a          716 c          768 g          429 t
ORIGIN      
        1 aacccggccg aggtacatga cagcgacccg gtcggaaatg tgctcgacga ctgcgagatt
       61 gtgggcgacg aaaagcagtg tcaggttcag ctcttcctgc aactcggtga ggagattgag
      121 aacctgcgct tggaccgaca cgtcgagcgc cgaaacgggc tcgtcggcca ccaccaggtc
      181 tggcttcagg atgagggcgc gggcgatgcc gatgcgctgc cgttgtccac cagagaactc
      241 atgggggtac cggctggcat gtgaacctcg gaggccgacg agctccagca tctccttgac
      301 ccgctcctgc tgttcgtcct tgtcaccaat gccgtgcaga cgcaaccctt ccgcgatggc
      361 cgggccgacc tgcgacctcg gatcgagcga gccgtaaggg tcctggaaga tgatctggac
      421 acgacgtcgg aatggtttga ggtcgttgcc cgagagatgg gtgatatcaa caccgtcgaa
      481 ttggatcgtc ccggaggtcg gctccagcaa acgagtcagc aggcgcccga gagtggtctt
      541 gccacagccc gactcgccga cgaggcccag agtctccccg ttgacgatgt cgaggtcgac
      601 tccggctact gctcggacgg tggccacctg acgttgcagg atgccccccc gaacggggaa
      661 ctccttgacc aggtcgcgga tctcgaccag ggtctcagcc atcggggtcc tcgaccttct
      721 ctggtccgta gtagagccaa cagcggacct tgtgcccgtc ctcgacttct cggaggtctg
      781 gctcgatctc agtgcagatc tgtagcgagt tctcaactcg gggaatgcag cgcggtgcaa
      841 accggcagcc cttggggagg tcgatcatcg agggcacgac accggggatc acgtccagct
      901 gctctctgag cgaccccagc accgggatcg agccgatcag acccttggtg taggggtgca
      961 gcgggttcct gaatatggag cggacatctg tctcctccac gatctcgccg gcgtacatga
     1021 ccgacacgcg gtccgccatc tcggccacga tgcccaggtc atgggtgatc aggatgaccg
     1081 aggtattcga ggtcttttgg aggtcccgga tgaggtcgag tatctgggcc tgaatggtca
     1141 cgtcgagagc cgtcgtcggc tcgtcggcga tgagcaactc gggctcgcac gccagggcca
     1201 tggcgatcat gatcctctgc gccatccctc cggaaagctc gtgcgggtat gcgtcggccc
     1261 gacgcttcgg atcggggata ccgaccttgg tcaggacctc gatgaccttc tcttcctcga
     1321 cttcggcgct ccagtcacga tgaagctcga agacttcttt gatctgggca ccggcccggt
     1381 acaccgggtt aagagctgag gtcggctgct ggaagatcat cgagatgtgc tcaccgcgca
     1441 aggctcggac atcgtccgcc ttcatcttgg cgatgtcctt gcctccgaag aggatctccc
     1501 cggcaaccac cttcccgggg tgagggatca agcccatgat cgagagcgag gtaaccgact
     1561 tcccgcatcc cgactcgccg acgagaccga ggacctctcc gcgcttgacg tggaagtcgg
     1621 cgccgtccac agctttgacc actccctgcc tcgtatcgaa gtgtgtcttg agccctcgca
     1681 catcgaggag aacctcgccc gcaggcacgg gcaacggcgc gtcggtgtcg atggcgctct
     1741 ccgggagagg tctggccgcg ctcacttgtt cagcgtgggg tcgagggcat cccggaggcc
     1801 gtcacccatg aggttgaaac cgaggacggt gatcatgatc gcgagcccgg ggaagatcat
     1861 gagatgaggg gcggtgaaga tcctgtcgcg ttcctcggcc agcatcgagc cccattcggg
     1921 agtcgggggg actgcaccaa gcccgaggaa tccaagggcg gctgcctcca ggatcacggt
     1981 ggcgatgccg agggtgccga ggacgactag tggtgtcagt gcgttgggga ccacccgggt
     2041 gacgagcact ttccatcggg acgcgccaag agccctagat gcggatacgt agtcgacctc
     2101 cttgatggaa agcaccgacg ccctcatcac ccgtgcgtac tgggggatgg tgacgatggc
     2161 gatggccagc agcgtgttgc ggaggcctgg tccgaggacg aagacgatgg cgatggccag
     2221 gatgagggac gggaaaccga gaacgacgtc catgatccgc atcatcacgt tgtcactcca
     2281 ccctcctgcg tagccggcga tggccccgat cacggtcccg atgacgatcg tgatgaggac
     2341 ggtcgtgaag ccgag
//
LOCUS       DASTXO010000553         2216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_512882, whole genome shotgun sequence.
ACCESSION   DASTXO010000553 DASTXO010000000
VERSION     DASTXO010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512882"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VFU96_11770"
     CDS             <1..353
                     /locus_tag="VFU96_11770"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEU5113987.1"
                     /translation="LMRHEQEIVPEGLYPYFQDVYDHILRISESSDSLRDLVTAIVET
                     NFSLRDYRQNLIVKKVSGWAAIIAVPALITGYYGMNVPYPGSGELTGVIWSTLLIIGV
                     SVGLYLVFRHNDWL"
     gene            501..1925
                     /gene="glnA"
                     /locus_tag="VFU96_11775"
     CDS             501..1925
                     /gene="glnA"
                     /locus_tag="VFU96_11775"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013874430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEU5113988.1"
                     /translation="MSKTAADVLSLVADEGYEFVDLRFCDLPGQVQHFTVPVKQLTED
                     SFEDGFGFDGSSIRGFQQIQESDMILIPDPGTAIDDPFRARKTLILYCFVNDPITGTP
                     YDRDPRYVARKAEEYLISTGLADTSYWGPEAEFYIFDDVRFGQNQFSGYYFVDSVEGA
                     WNSGEFEEGGNLGYKPRYKEGYFPVPPTDHFQDLRSEMTANLEAAGIEIEVQHHEVGT
                     AGQAEIDMRYDTLLRTADNVTLYKYIVKNTAWEYGKSVTFMPKPIFEDNGSGMHTHQS
                     LWKDGEPLFFDEAGYAGLSDMARWYIGGLLKHAAAILAFAAPTTNSYHRLVPGYEAPV
                     NLVYSSRNRSAAIRIPQYSQAPGAKRLEFRCPDPSCNPYLAFSAMLMAGLDGVQNRIE
                     PHEPVDKDIYDLPPAELAGLPSVPGSLTEALDSLKADHEFLLQGGVFSQELIEAWIDY
                     KIEHEVDPVRLRPHPYEFHLYYDI"
     gene            complement(2022..>2216)
                     /locus_tag="VFU96_11780"
     CDS             complement(2022..>2216)
                     /locus_tag="VFU96_11780"
                     /inference="COORDINATES: protein motif:HMM:NF024920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol carrier protein domain-containing
                     protein"
                     /protein_id="HEU5113989.1"
                     /translation="AACAPTTDEPTVLLDLEDLSAGYMGRPRFRQWARVGRLSGAGDA
                     LAALDAAFTWDPQPWCPEIF"
BASE COUNT          414 a          736 c          654 g          412 t
ORIGIN      
        1 cattgatgcg tcatgagcaa gagatcgttc ccgaggggct ctacccctac ttccaggacg
       61 tctacgacca cattctccga atcagcgagt cttccgactc cctgcgcgac ctcgtcaccg
      121 cgatcgtcga gaccaacttc agcctccggg actaccggca gaacctgatc gtgaagaagg
      181 tgtcgggttg ggcggcgatc atcgcggtcc cggcgctgat caccggctat tacgggatga
      241 atgtccccta ccccgggtct ggcgagctga ccggtgtcat ctggtcgacc ttgctgatca
      301 tcggcgtctc ggtaggtctc tatctcgtct tccgccacaa cgattggctc tgaactgccc
      361 tgtggggccg cggtttcact cgcgacgagg catgagagca actccgaaac acagcgttca
      421 tgatccggca acggcatgga aacggggaac gggctaaaaa ctctccgttc acgcctgtca
      481 acccaaccga aaggagcacc gtgagcaaga cggctgccga cgtcttgtcc ctcgtcgccg
      541 atgaggggta tgagttcgtc gatcttcgct tctgcgacct gcccggccag gtccagcact
      601 tcacggtccc ggtcaagcaa ttgaccgaag attcgttcga ggatggtttt ggattcgacg
      661 gctcgtcgat tcggggcttc cagcagattc aggaatcgga catgatcctg atcccggacc
      721 cgggcaccgc catcgacgac ccgttccgtg cccggaagac cctcatcctc tattgcttcg
      781 tcaacgaccc gatcaccggc accccctatg accgtgaccc cagatacgtt gcgaggaagg
      841 ccgaggagta cctgatcagc accggcctgg ccgacacctc gtattggggc ccggaggccg
      901 agttctacat cttcgacgat gtccgcttcg ggcagaacca gttctctggc tactacttcg
      961 tcgactcggt ggagggtgcc tggaactcgg gcgagttcga ggaagggggc aacctcggtt
     1021 acaagcccag atacaaggaa ggctatttcc cggtcccacc caccgaccat tttcaggacc
     1081 tccggtcgga gatgacggcc aacctcgagg cggccggcat cgagatcgag gtgcagcatc
     1141 acgaggtggg taccgcaggc caggcggaga tcgacatgcg ttacgacacg ctgctccgca
     1201 ccgccgacaa cgtgacgctc tacaagtaca tcgtgaagaa cacggcttgg gagtacggca
     1261 agtccgtcac cttcatgccc aagccaatct tcgaggacaa cgggtcgggc atgcacaccc
     1321 accagtcgct gtggaaggac ggcgagcctc tgttcttcga cgaggctggt tacgccggtc
     1381 tctccgacat ggcccggtgg tacatcggag ggttgttgaa gcacgccgca gcgatcctcg
     1441 ccttcgccgc tccgactacg aactcctatc accgcctggt gcccggttac gaggcaccgg
     1501 tgaacctggt gtactccagc cggaaccggt cggcggcgat caggatcccc cagtactcac
     1561 aggctcccgg ggccaagcgc ctcgagttcc gctgtccgga cccctcatgc aacccgtacc
     1621 tcgccttctc cgccatgctc atggccggtc tcgacggagt ccagaaccgg atcgagcccc
     1681 acgagccggt cgacaaggac atctacgacc tgccgcctgc cgagctcgcc ggtctcccct
     1741 cggtgccggg cagtctgacg gaggccctgg attccctgaa agcggatcac gagttcctgc
     1801 tccagggcgg cgtcttctcc caggagctga tcgaggcctg gatcgactac aagatcgagc
     1861 acgaggtcga ccctgtccgt ctccgccctc acccctacga gttccacctc tattacgaca
     1921 tctgagatgg ggccctcgcg gcctcatcgc acgtctcacg tcctacgttc tgcgtagtac
     1981 gtgggccgag ggtccgtcgg cagacgggcc ctcgtgctgc ctcagaagat ctccgggcac
     2041 cagggctgcg ggtcccaggt gaaggcggcg tccaaggcgg ccagggcatc gcctgctcca
     2101 ctcaaacggc cgacgcgggc ccactgtctg aaccggggcc gtcccatgta acccgcgctg
     2161 agatcctcga gatcgagcag gacagtgggc tcgtcggtcg tgggggcgca tgcggc
//
LOCUS       DASTXO010000554         2023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_586542, whole genome shotgun sequence.
ACCESSION   DASTXO010000554 DASTXO010000000
VERSION     DASTXO010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586542"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VFU96_11785"
     CDS             complement(<1..421)
                     /locus_tag="VFU96_11785"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEU5113990.1"
                     /translation="MRTLPRIEVLPGVVRRPADFDTGALTGEILEEVRAGGEPAIRRF
                     SEHFGDLAPGEQIVHERSDLEKSLDRVGPEERKTLERVASRIRHFAEAQREALVDVDV
                     EVTGGRAGHRWVPVASVGAYAPGGRYPLPSSVLMTVIP"
     gene            complement(418..1683)
                     /gene="hisE"
                     /locus_tag="VFU96_11790"
     CDS             complement(418..1683)
                     /gene="hisE"
                     /locus_tag="VFU96_11790"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEU5113991.1"
                     /translation="MIIPSIDISKGQAVQLVGGETVAIEAGDPIPWLDRFGVTGEVAV
                     IDIDSARGDGDNRDLISELCRRGRVRVGGGIRDVPTALEWLDLGAEKVIIGTAATPGL
                     LRELPADRAIVALDSRDGEVLTHGWRRSSGDDLMTRVEELRGLCDGFLVTFVELEGRL
                     GGTDVDRAREVITAAAPARVTIAGGISTPEEIAALDRMGADAQVGMALYSGRLTSGQA
                     VAATMRSDRADGLWPTVVTDEAGVALGLVYSSAESLERAVTERRGIYHSRSRGVWVKG
                     ETSGATQDLLAVDIDCDRDTLRFTVRQSEPGFCHTDTRTCWGEDHGVGRLERRLGRIA
                     RSDDPASNTMKLLADPKLLAAKLTEEASELARAETTAEVIHEAADLLYFLLVRATAAG
                     VELEDVALELDRRERRTTRRPMTTKESLP"
     gene            complement(1680..>2023)
                     /locus_tag="VFU96_11795"
     CDS             complement(1680..>2023)
                     /locus_tag="VFU96_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018141079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEU5113992.1"
                     /translation="WEVVIESGRSRTGVDVVEWCRKATTLGAGEVLLTSWDRDGTRQG
                     YDLALIAAVSDAVTVPVIASGGANEPGHLIDALRAGASAALVASILHDADTTVEALKS
                     ALAGAGILVRP"
BASE COUNT          335 a          721 c          624 g          343 t
ORIGIN      
        1 ccgggatcac ggtcatgagt accgaagagg gcaggggata tcgccctcca ggcgcgtaag
       61 cgccaaccga tgccaccggt acccagcgat gcccggcacg tcctccggtc acctcgacgt
      121 cgacgtcgac cagggcctca cgctgtgcct cggcgaagtg cctgatccgt gaggccactc
      181 tttccagtgt cttgcgctct tcagggccaa cccggtcgag ggacttctcc aggtcgcttc
      241 gctcgtggac gatctgctca cctggggcca ggtcgccgaa atgctcggag aatcgacgta
      301 tcgcaggctc gccgcctgca cgcacctcct cgaggatctc gcctgtgagc gcgcccgtat
      361 cgaagtcggc agggcgtcga acgaccccgg gcaggacctc gattcggggc aatgttctca
      421 tggcaggctc tccttcgtgg tcatcggccg acgggtggtc cggcgctcac gccggtccag
      481 ctctagggcc acgtcttcga gctcgacccc cgcggctgtt gccctcacca ggaggaaata
      541 gaggaggtcg gcagcctcat ggatgacctc cgcggtggtc tcggcccggg ccagctcgga
      601 tgcttcctcg gtgagcttgg cggcgagcag cttcgggtcc gcgaggagct tcatggtgtt
      661 ggaagccgga tcatccgatc gggcaatccg gccgagacgt cgctccagac ggcccacgcc
      721 gtggtcctcg ccccaacatg tcctggtgtc agtgtgacag aagccgggct cgctctgtct
      781 gacggtgaaa cggagcgtgt cgcgatcaca atcgatgtca accgccaaca ggtcctgggt
      841 ggcacccgac gtctcgcctt tgacccagac tcctctcgag cggctgtgat agatgccgcg
      901 cctctcggtc acagctcgct ccaggctctc cgcgctcgag tagaccagcc caagcgcgac
      961 gccagcctcg tccgtcacga ccgtgggcca caagccgtct gcccgatcgg agcgcatcgt
     1021 cgcggcgaca gcctgacccg acgtcagccg gcctgaatag agggccatcc ccacctgcgc
     1081 gtcggcaccc atccgatcga gggcagcgat ctcctccggt gtgctgatcc cacccgcgat
     1141 cgtgacacgg gcaggcgcgg cggcggtgat cacctctctg gcacgatcta cgtcggtgcc
     1201 ccccagccga ccttccaatt ccacgaaggt gacgaggaat ccgtcgcaaa gcccacgaag
     1261 ctcctcgacc cgagtcatca gatcatcacc gctgctccga cgccatccgt gggtcaggac
     1321 ctcaccgtcc cgtgagtcga gagcgacgat tgcacggtcg gcggggagct ctcggagcaa
     1381 cccgggtgtc gccgcggtgc cgatgatgac cttctccgcg cccagatcca gccactccaa
     1441 ggcggtgggc acatcacgga tcccgccgcc gacccggacc cgtccgcggc ggcacaactc
     1501 ggagatcaga tcccggttgt cgccatcgcc cctcgcggag tcgatgtcga tcacggccac
     1561 ctcaccggtc acaccgaaac ggtccaacca cggaatcggg tcaccggcct cgatggcgac
     1621 cgtctcaccg ccgaccagct gcacggcttg ccctttcgag atgtcgatcg aagggatgat
     1681 catggccgta ccaagattcc ggcgccggcc agcgcgctct tgagtgcttc cacggtggtg
     1741 tcggcatcgt ggaggatgct tgccacgaga gccgccgagg cgccggctcg gagcgcgtcg
     1801 atcaagtgtc cgggctcgtt tgccccaccg gacgcgatga ctggcaccgt cacggcatcg
     1861 gagactgctg cgatcagggc gaggtcatag ccctggcgcg tcccgtcccg atcccagctg
     1921 gtgagcagga cctcaccggc gcccagggtc gttgcctttc ggcaccattc cacgacatcg
     1981 acaccggtgc ggctccgtcc gctctcgatg accacctccc atc
//
LOCUS       DASTXO010000555         2343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1360645, whole genome shotgun sequence.
ACCESSION   DASTXO010000555 DASTXO010000000
VERSION     DASTXO010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360645"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VFU96_11800"
     CDS             complement(<1..231)
                     /locus_tag="VFU96_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 2,3-dioxygenase"
                     /protein_id="HEU5113993.1"
                     /translation="MTIRSNLDVAHLGYVELFTPTFDESLRFFTDVLSMEVVDSVEDT
                     SYLRAWDDYERFSLKLTARSEAGIGRVGVRASD"
     gene            complement(228..1256)
                     /locus_tag="VFU96_11805"
     CDS             complement(228..1256)
                     /locus_tag="VFU96_11805"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU5113994.1"
                     /translation="MTEQYTEATGTSVIGGTERRASRSLSALLLPIAVIGAALALVPF
                     WLGDSRVLMGVAVLGLTFACYTIGFNLIFGSTGQLFLCVGALAGIGGYGGAILADTVG
                     LPMILSIILATIAAGLVGALLSWIAVRRSLGVIFTGIITLIFALAFENLLLGLGGVTG
                     GESGFSISSGADGLLRRQIPPYYVMLGLVLVFLLAHGLLRRSHVGWAFRALRDDEVAA
                     ELTGVDVARYRVYAALIGAAMLGMAGATYAFSEGRISPTTFGFAQVDVVVLVMLAFGG
                     IGTLFGPILGAITFTAIDEVLIELGQLRVLVYGLLIIVLFLWIPRGVIPTLGDLLRRQ
                     ESPGRREE"
     gene            complement(1253..2110)
                     /locus_tag="VFU96_11810"
     CDS             complement(1253..2110)
                     /locus_tag="VFU96_11810"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU5113995.1"
                     /translation="MIANVLDGVVLGLQFGLLAAGLTLVYGLGGVLNLAYGQMAVLSA
                     MAVLFAIDAGVPVLPAVGIGLAVGVVLGLILDLTILRPIYRLQGEARVLLSLLLTLGV
                     AFIVDGYLTWRYPTEGLTLRIGGGPMDIIGVPMRRGSIVASGITLVALVALFAFFRGT
                     TLGRAVRSVIEDEEGARLVGIDPRRMLRIILALSGFLAALVAVTQSMVRPITVSAGFS
                     ITVFALIVTVVGGLGSVTGAMLAGIILGTISNIAASYIGTFLSLIILLGAAAVTILIR
                     PSGLLGVRE"
     gene            complement(2107..>2343)
                     /locus_tag="VFU96_11815"
     CDS             complement(2107..>2343)
                     /locus_tag="VFU96_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5113996.1"
                     /translation="LDEPTTGLAPKLAAEAYQALGVLRGRGLTVLVAEQQVPLAMAFS
                     DRGYVLENGRIELEGTSEELATNPDVRRSYLGVT"
BASE COUNT          508 a          768 c          751 g          316 t
ORIGIN      
        1 gtcggaggcc ctcacaccga cccgaccgat cccggcctcg gagcgggccg tcagcttgag
       61 gctgaagcgc tcatagtcgt cccaggcgcg gaggtaggag gtgtcctcga cggagtcgac
      121 gacttccatt gagagcacgt cggtgaagaa gcggagactc tcgtcgaacg tcggcgtgaa
      181 caactccacg tatccgagat gggcgacgtc gagattcgat cggatggtca ctcctcccgt
      241 cggccggggc tctcttggcg acgaagcaag tctcccaggg tcgggatgac cccacggggg
      301 atccatagga acaacacgat gatgaggagc ccgtagacga gcactcgaag ctggccgagc
      361 tcgatcaaga cctcgtcgat ggccgtgaac gtgatcgccc cgagtatcgg accgaaaagg
      421 gtgccgatgc cgccaaaggc cagcatcacg agaacgacga cgtcgacctg ggcgaaccca
      481 aaggtggtgg ggctgatccg gccttcgctg aaggcatacg tagccccggc catgcccagc
      541 atcgccgctc cgatcagagc ggcgtagacc cgatagcgcg ccacatcgac accggtcagc
      601 tcggcggcga cctcgtcgtc acgcaaggcg cgaaatgccc agccgacgtg tgaccgtctc
      661 agcaagccgt gagcaagcag gaacaccaac accagtccca gcatcacgta gtagggcgga
      721 atctggcgtc gcaggagccc gtcggctccg gacgagatgg aaaagcccga ctcgcctccg
      781 gtgacaccgc ccagcccgag caagagattc tcgaaggcga gggcaaagat gagggtgatg
      841 atcccggtga agatgacgcc caatgagcgt cgcactgcga tccagctcaa caacgcccca
      901 accaggccgg cggcgatggt cgccaggatg atgctcagga tcatggggag cccaacagtg
      961 tcggcgagga tggcgccgcc gtagcccccg atcccagcca gggcacccac gcagaggaag
     1021 agttggccgg tgctgccgaa gatcagattg aaaccgatcg tgtagcaagc aaaggtgaga
     1081 ccgaggacgg caacgcccat caggactcgg gagtctccga gccagaaagg cacgagtgct
     1141 agggctgccc cgatgacggc tatcggcagc aacaacgccg acagggatcg cgaggctcgg
     1201 cgctcggtgc ctccaatcac cgaggtgccg gtggcttcgg tgtactgctc cgtcattccc
     1261 tcactccgag gagcccggac ggtcggatga ggatggtcac cgcggctgca ccgagcagga
     1321 tgatcagcga caggaaggtc ccgatgtacg acgcggcaat gttgctgatg gtcccgagga
     1381 tgatcccggc gagcatggcc ccggtgacac tccccagtcc cccgaccacg gtgactatca
     1441 gggcgaacac ggtgatgctg aacccggccg acacggtgat cggccggacc atcgactggg
     1501 tgaccgccac cagggcggcg aggaaaccgc tcagggcaag gataatgcga agcatgcgtc
     1561 gcggatcgat cccaaccaga cgtgccccct cttcgtcctc gatcaccgag cggacggccc
     1621 gacccagcgt ggtcccccgg aaaaacgcaa atagtgcgac gagagcgacc agggtgatgc
     1681 cagacgccac gatgcttccc cgccgcatcg ggaccccgat gatgtccatc gggcccccac
     1741 caatacgcaa agtgagcccc tcggtgggat accgccaggt gaggtacccg tcgacgatga
     1801 aggccacacc gagcgtgagc agcaagctga gcaggaccct cgcctcaccc tggagccggt
     1861 atatcgggcg gagaatggtc aggtccaata tcagcccgag cacgacaccg actgccagcc
     1921 cgatgccaac cgcgggcaag accggcactc cggcgtcgat cgcgaacagg accgccatgg
     1981 cggagagaac cgccatctgc ccgtaggcca ggttgagcac accacccaac ccgtagacca
     2041 gggtgagccc ggccgcgagc aggccgaact gcagcccgag cacgaccccg tcgagaacgt
     2101 tggcgatcac gtcacaccga gataagagcg ccggacgtcc gggttcgtcg ccagctcctc
     2161 ggaggtgccc tccaattcga tcctgccgtt ctccagcacg taaccgcgat cgctgaaggc
     2221 catcgcgagc ggcacctgct gctcggcgac cagcaccgtc agccctcgcc cccgaagcac
     2281 accgagggcc tgatatgcct ctgcggcgag tttcggggca agaccggtgg tgggctcgtc
     2341 gag
//
LOCUS       DASTXO010000556         2983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_475820, whole genome shotgun sequence.
ACCESSION   DASTXO010000556 DASTXO010000000
VERSION     DASTXO010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475820"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VFU96_11820"
     CDS             <1..648
                     /locus_tag="VFU96_11820"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEU5113997.1"
                     /translation="ELTRQIEPGSVDLVVWSEGSTGSFNADPVQNPEIGEAIGAEARR
                     IGAWFLVGSDRPVSETHWINANVVFNLGDGVAMGSVISFEGGFSRYPRQNAQAGANLI
                     VVATNEGSYGTTRGSDQFIGMTRMRAAELGTPVIHAAVTGKSVFIGDGGQFTSESTGL
                     GTMEILIGAVAPSARSIYANTGDVVMIVAALIGLGTWWRARHPLVVSAPVIDEEE"
     gene            655..2604
                     /gene="acs"
                     /locus_tag="VFU96_11825"
     CDS             655..2604
                     /gene="acs"
                     /locus_tag="VFU96_11825"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016825247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEU5113998.1"
                     /translation="MSQALENLLTEDRKFEPTPEFAANANARPGIHEEAAADHLAFWH
                     DQALERITWHKEPTQVLDDANAPFFKWFADGELNVSFNCLDRHLDEHGEQVAYHWVGE
                     PGDSRSLTYRDLHSEVCRFANGLLSLGVEKGDRVAIYMGMVPELPIAMLACARLGLVH
                     SVVFGGFSSDALSSRILDADARVVITQDGAWRAGNVVQLKANADVALASTPDVRHVVV
                     LKRTAHGVDMTEGRDIWWHDLVEDQADDCEPVVLGAEDPLYILYTSGTTGRPKGIVHT
                     QGGYLTGIVTTHHHVFDLKPDTDVYWCGADIGWVTGHSYIVYAPLSNRTTSVMYEGAP
                     NFPDLDRLWQIIEDHGVTIFYTAPTAIRTFMKWGDQHVAKHDLSSLRLIGTVGEPINP
                     EAWIWYQQTIGGGKCPVVDTWWQTETGAIMISPLPGVVSTKPGSATVPFPGIFADVVD
                     DAGESVPLGGGGYLVITKPWPAMARTIYGDDARYEDTYWSRFPGRYFPGDGAKRDDDG
                     YFWLLGRVDDIMLVSGHNISTTEVESALVSHVSVAEAAVVGRRDEITGQAIAAFVTLR
                     AGVEGDEDLLQELRDHVANTLGPIAKPKSIVFTDDLPKTRSGKIMRRLLQDISEQRQL
                     GDVTTLANEAVVHEIAEKAAETTED"
     gene            complement(2612..>2983)
                     /locus_tag="VFU96_11830"
     CDS             complement(2612..>2983)
                     /locus_tag="VFU96_11830"
                     /inference="COORDINATES: protein motif:HMM:NF016213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEU5113999.1"
                     /translation="LWAIVRGDAVAWAVAIAALLLAGIGTFIKYSIPKQRLNEVGIPN
                     RTLLIATAVAIVGLFVIPVVGAPIGFVLAIYISERLRVGADRAWPATKTSLGAVATSI
                     GIELATGLLIAAIWFGTVLLA"
BASE COUNT          565 a          973 c          961 g          484 t
ORIGIN      
        1 gagctgaccc ggcagatcga gcccggctcg gtggacctgg tcgtctggtc cgaggggtcg
       61 acgggctcgt tcaacgccga cccggtgcaa aaccccgaga tcggggaggc catcggcgcc
      121 gaggcgcgac gaatcggtgc ctggttcctg gtggggagcg atcggccggt gagcgagacg
      181 cactggatca atgccaacgt cgttttcaac ctgggagacg gggtcgcgat gggctcggtc
      241 atctccttcg aaggagggtt ttccagatat ccccggcaga acgcccaggc gggggcgaat
      301 ctgatcgtgg tcgcgacaaa tgaggggtca tacgggacaa cccggggatc agaccaattc
      361 atcgggatga cgcggatgcg ggcagccgag ttgggcactc ccgtgatcca cgccgcagta
      421 actggcaagt cagtgttcat cggagatgga ggtcagttca cctcggagag caccgggttg
      481 gggacgatgg agatcctcat cggcgcggtg gcgccttcgg cccgctcgat atacgccaac
      541 accggcgacg tcgtgatgat cgtcgctgct ctcatcgggc tgggcacctg gtggcgggca
      601 cggcatcccc tggtagtttc cgcccccgtc atcgacgagg aggagtgaga accgatgtcc
      661 caggccctgg agaacctgct caccgaggac cggaagttcg agcccacccc cgagttcgcc
      721 gcgaacgcaa acgcccggcc cgggatccac gaggaggctg cagccgacca tctggctttc
      781 tggcatgacc aggccttgga gaggatcacc tggcacaaag agcccaccca ggtcctcgac
      841 gacgcgaatg cgcctttctt caagtggttc gccgatggcg agctcaacgt ctcgttcaac
      901 tgcctcgacc gtcatctcga cgagcacggt gagcaggtgg cctatcactg ggtcggcgag
      961 ccaggcgaca gccgcagcct gacctatcga gatctccact ccgaggtgtg ccggttcgcc
     1021 aatgggctcc tcagtctcgg cgtcgagaag ggcgatcggg tcgccatcta catggggatg
     1081 gtccccgagc tccccatcgc catgctcgca tgcgcccgtc tcggcctggt ccactccgtg
     1141 gttttcggtg gattctcgtc cgacgccctc tccagccgga tcctcgatgc cgacgcccgc
     1201 gtggtgatca cccaggacgg tgcctggcgc gccggaaacg tcgtccagct gaaggccaac
     1261 gccgacgtgg ccctggccag cacgcccgac gtcaggcacg ttgtcgtcct gaaaaggact
     1321 gctcacggtg tcgacatgac cgagggcagg gacatctggt ggcacgacct agtcgaagac
     1381 caggccgacg attgcgagcc agtcgtgctc ggggcggaag acccgttgta catcctctac
     1441 acatcgggga cgacgggacg gcccaagggg atcgtccaca cgcagggggg ctatctgacc
     1501 gggatcgtga ccacacacca tcacgtcttc gatctaaagc ccgacaccga tgtctactgg
     1561 tgtggcgccg acatcgggtg ggtcaccggg cacagctaca tcgtctacgc ccccctctcc
     1621 aaccgaacca cgagcgtcat gtatgagggc gcgcccaatt tccccgacct cgaccgcctt
     1681 tggcagatca tcgaagacca cggggtcacg atcttctaca cggcgccgac agcgatccgc
     1741 accttcatga agtggggcga tcagcacgtc gccaagcacg acctgagctc gctgcggctg
     1801 atcgggaccg tcggcgagcc gatcaacccc gaagcgtgga tctggtacca gcagaccatc
     1861 ggcgggggaa aatgcccggt ggtcgacacc tggtggcaga ccgagaccgg ggcgatcatg
     1921 atcagcccac ttcccggtgt tgtgtccact aagccggggt cggcaaccgt tcccttcccg
     1981 ggcatcttcg ccgacgtcgt ggacgatgcc ggcgagtcgg tgccgctcgg agggggcggc
     2041 tatctcgtca tcaccaaacc ctggccggca atggcgcgaa ccatctatgg cgatgacgcg
     2101 cggtacgagg acacctactg gtcgcggttt cccggaaggt acttccccgg agacggcgcc
     2161 aagcgcgacg acgacggcta tttctggctg ctgggacggg tcgacgacat catgctggtc
     2221 tcggggcaca acatctccac caccgaagtg gagtcggcac tggtctccca cgtctcagtt
     2281 gccgaggccg cagtcgtcgg ccgccgcgac gagatcaccg gccaggccat cgccgccttc
     2341 gtgacgttac gggccggtgt cgaaggcgac gaggacctcc tccaggagct gagggaccat
     2401 gtcgccaaca ccctggggcc aatcgccaag ccgaagtcca tcgtcttcac cgatgacctg
     2461 cccaagacac ggtcggggaa gatcatgaga cgcctgctcc aggacatctc tgagcagcgc
     2521 cagctcggcg acgtgacaac cctcgccaac gaggccgtcg ttcacgagat cgcagagaag
     2581 gccgccgaga cgaccgagga ctgactaccg gctaagccag caggaccgtc ccgaaccaga
     2641 tggcggcgat gaggagcccg gtggcgagct cgatcccgat cgaggtggcg acggcgccga
     2701 gactggtctt ggtggccggc caggcacggt ccgcacccac cctcaggcgc tcgctgatgt
     2761 agatggccag aacgaacccg atcggcgctc ccaccaccgg tatcacgaac agcccgacga
     2821 tcgccaccgc ggtcgcaatg agcagcgtcc ggttggggat cccgacctcg ttgagcctct
     2881 gcttggggat cgagtacttg ataaaggtcc cgattccggc gaggagcagg gctgcgatgg
     2941 cgacagccca ggcgacagca tcacccctga ctatggccca tag
//
LOCUS       DASTXO010000557         3515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_881743, whole genome shotgun sequence.
ACCESSION   DASTXO010000557 DASTXO010000000
VERSION     DASTXO010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881743"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VFU96_11835"
     CDS             <1..515
                     /locus_tag="VFU96_11835"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5114000.1"
                     /translation="NEPVPGQPDRKFDLLAAFEVLEHIEDDRGTLSSWVRWLNPGGHV
                     LISVPAHPDRFGPCDEMAGHCRRYERKSLASLLRSSGLEPLSIESWGMPLGYLLEAGR
                     NVLARRRLDKDVGTAGSGRFFQPPAALGRTVELALRPAAFAQRPFHDTEMGIGYVAVG
                     RLADARTEGE"
     gene            512..1636
                     /locus_tag="VFU96_11840"
     CDS             512..1636
                     /locus_tag="VFU96_11840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5114001.1"
                     /translation="MRVLWTGTFDPEFSRNKKLARLMRLSGVRWRVLRENVWATDRVA
                     LASRPSLVVALRALIGYPKLLVRLLMAPAPDVYLVSYPGWFDVPIVRLAASIRRRPLV
                     FDPFLSLFDTMISDRGIRSGTSLVGRLASAIDRWSLRLADVVIADTEPQLAFYRQIAG
                     RLRSQGSVLAVGADDELFVPLPEVKSEPGRLLYYGNFIPLHGAATIVEAAALLESNGI
                     KLVMIGDGPDREVVDEAIARTGLSVERHGIIPLRELPEQVARATVCLGVFGGSQKAGR
                     VIPHKVYEALAMRKPVVTREGPAVSAAFPEGGVVTVPPQDAAALADAVRGLIEDPPRR
                     EKVAAAGHQSYRDRFQERALAADLFVVLEASVQSRGSVGA"
     gene            complement(1583..2596)
                     /locus_tag="VFU96_11845"
     CDS             complement(1583..2596)
                     /locus_tag="VFU96_11845"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HEU5114002.1"
                     /translation="MSETPIPPGSAGKRRSRLRSWAGWLVVGAIFFFIARSLVRNWAD
                     LAAADLHFDFWLLLASFALLGLWMVAQAFIWHLLTVTSGAHIPLPKAMAAWFYSQLGK
                     YVPGKVFLYLGRVHLYTREGRGAGPITVAFGVEFVGNLAAAVLTVLVAALTSDVPALD
                     DYRWLLLIALVAFLIALHPRFMGWAVKLAARALRRQPFQIDLSYRQVLGYVSLYFVNW
                     LLFGVALYVFIRSFYSLELSSILYLSGAFSLSSVIGILALFAPSGLGVREGILAIFLN
                     QVMPTSIALVVSVASRLWITVAELAAAGIVYLFVRPGRPGVEPVSGADRTSALDAGLE
                     DDE"
     gene            2719..3237
                     /locus_tag="VFU96_11850"
     CDS             2719..3237
                     /locus_tag="VFU96_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5114003.1"
                     /translation="MPKPDHPRAWRQRLGTVYTRYLPVALGLTAVVNLALTPFLPGSA
                     APIRTHPDGSLTELVHTEQRAVDRRFGFYMELDDASDGGILIVPRGSFVNPELAEGFA
                     GFEVLEAEYDPRAGADSLPETPERWLVETDSGDLVFSIVPGSDNVWWLAERPNGEVVV
                     VPNSVVPAPVSP"
     gene            3234..>3515
                     /locus_tag="VFU96_11855"
     CDS             3234..>3515
                     /locus_tag="VFU96_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5114004.1"
                     /translation="MINEIALAAALTISGSFLLTAVRRDVTAVARCWLSMPIGAAAYL
                     LVALVSLVFFDTLDPVITLLVVLGLGLVVVAVSLSKGGLGRDDLLWMMIG"
BASE COUNT          646 a         1111 c         1140 g          618 t
ORIGIN      
        1 gcaacgagcc tgtgcctgga cagccggatc gcaagttcga tctgctggcc gccttcgagg
       61 tgctcgagca catcgaggat gatcggggaa cactcagtag ctgggtgcgg tggctgaacc
      121 caggcggaca cgtcctcatc agcgttcccg cccatcccga ccgcttcggg ccgtgcgatg
      181 agatggcagg ccattgccgc cgttatgagc gaaagagttt ggcgtcgttg ctccggtcgt
      241 ccggactcga gccactctcg atcgaatcgt gggggatgcc cctcggatac ctgctggagg
      301 cagggcggaa cgtcctggcc cggcgccggc tcgacaagga cgtaggcacg gcagggagtg
      361 gtcggttctt ccagccacca gccgctctgg ggcgaacggt ggagctggcg ctgcgtcccg
      421 cggcgttcgc ccagcggccg ttccacgaca ccgagatggg catcggatat gttgcggtcg
      481 gccgtctggc agacgcccga accgagggcg aatgagagtc ctctggacag gcacattcga
      541 ccctgagttc tcccgcaaca agaagctggc tcgactgatg cgcctctctg gggtccgctg
      601 gcgtgttctc cgtgagaacg tatgggcaac cgacagagtg gccctggcct cgcggcccag
      661 tctcgtcgtg gcgctgaggg cgctgatcgg ttacccaaaa ctgttggtac gcctgctgat
      721 ggcccccgca ccggacgtct acctggtctc ctatccgggg tggttcgacg tgccgattgt
      781 tcgcctcgcg gcatcgatac ggcgaagacc gctggtcttc gacccgtttc tgtccctctt
      841 cgacacgatg atctccgacc gtggcatccg cagcggcacg tcattggtcg ggcggctggc
      901 atcagccatc gatcgctggt cattacgact cgccgacgtg gtgatagccg acaccgaacc
      961 acagctcgcc ttctatcggc agatcgccgg ccgactccga agccaaggat cggtgctcgc
     1021 ggtcggggcc gacgacgagc tgttcgtacc gcttcccgag gtgaagtcgg agccgggtcg
     1081 cttgctgtac tacggcaact tcatacccct acacggcgcc gccacgatcg tcgaggcggc
     1141 agctctgctc gagtcgaacg ggatcaagct ggtcatgatt ggcgacggcc cggatcgtga
     1201 ggtcgtcgat gaggcgatcg ctcgcaccgg cctttcggtc gagcggcacg ggatcattcc
     1261 ccttcgggag ctcccggagc aagtggcgcg ggccacggtg tgtctcggcg ttttcggtgg
     1321 ctcgcaaaag gccggtcggg tcattccgca caaggtctat gaggcacttg ccatgcgcaa
     1381 gccagtggtg actcgggaag gtccagcagt cagcgcagca ttccccgaag gcggggtagt
     1441 cacggtgccg ccccaggatg cggctgctct cgccgacgcc gtccgcggtc tgatcgagga
     1501 tccacctcgg agggaaaaag tagccgccgc cgggcaccag tcctatcgag atcgcttcca
     1561 ggaacgagca cttgccgcgg atctattcgt cgtcctcgag gccagcgtcc agagccgagg
     1621 ttcggtcggc gcctgagacg ggttccacac cgggacgccc aggtctcacg aatagataga
     1681 cgatcccggc tgccgccaac tcggcaacgg tgatccacaa gcgggatgcc accgaaacca
     1741 ccagggcgat cgacgtcggc atgacctggt tgagaaagat cgccaggatc ccctcgcgca
     1801 ccccgagccc tgatggagcg aacaacgcga ggatcccgat gacactggag agtgagaaag
     1861 ctcccgatag atagaggatc gaggacagct cgagtgaata gaacgaccga atgaagacgt
     1921 acagggcgac gccaaacagc agccagttga cgaaatagag ggagacatag ccaagcacct
     1981 ggcggtagct cagatcgatc tggaatggtt ggcgacgcaa cgcccttgcc gccagcttca
     2041 cggcccaacc catgaatcgg gggtgaagcg cgatcaggaa cgccacgagc gcaatcaaca
     2101 agagccaccg ataatcgtcg agagccggca cgtccgaagt gagggcggcg acgagcacgg
     2161 tgagcacggc ggccgcgagg ttgccgacga actccacacc gaacgcaacg gtgatcggtc
     2221 cggcgccccg tccctctcgt gtgtacagat gcacgcggcc cagatagagg aacaccttcc
     2281 cgggcacgta ttttccgagc tgtgagtaga accaggcggc catcgccttg ggcagcggga
     2341 tgtgagcgcc gctcgtcact gtgaggagat gccagatgaa cgcctgggca accatccaca
     2401 gcccgagtag tgcgaacgac gcaagtagga gccagaagtc gaaatggaga tccgcggcgg
     2461 ccaggtccgc ccagttgcga accaggcttc gtgcaatgaa gaagaagatg gcgccaacga
     2521 ccagccaccc ggcccatgac ctgagccggc tcctacgttt cccggcactt ccgggtggaa
     2581 tcggggtctc gctcaatcgg atgtcccggt ggaggtcata ccggtcgcga cgttaggagc
     2641 catcccacgg gcggcccgtc aatgccagcc ctgtgactgg gatctggcga agcgaccaag
     2701 cgacggtatc gtcccggcgt gccgaagccg gatcacccca gggcatggcg ccagcgcctc
     2761 ggcacggtct acacccggta cctcccggta gcgcttggcc tgactgccgt ggtcaatctg
     2821 gcgctgacac cttttctgcc cggatcggct gccccgatac gaacccatcc cgacggttca
     2881 ttgaccgagt tggtccacac cgagcagcgg gccgtggatc gtcggtttgg gttctacatg
     2941 gagctcgacg atgccagcga cggagggatc ttgatcgtcc cgagggggtc gttcgtcaac
     3001 cccgagctgg ctgaaggctt tgccggcttc gaggtgctcg aggccgaata cgacccgaga
     3061 gccggagcgg acagtctccc ggagacgcct gagcgctggc tggtcgagac cgactcgggt
     3121 gatctcgtct tctcgatcgt cccgggcagc gacaacgtgt ggtggctcgc cgagcgaccg
     3181 aacggcgagg tcgtggtcgt ccccaactct gtcgtgcccg ccccggtgtc gccgtgatca
     3241 acgagatagc ccttgcagcg gccctgacta tttccggcag cttcctcctc accgcggtcc
     3301 gaagagatgt gaccgcggtc gcccgatgct ggctatcaat gccgatcggg gccgccgcct
     3361 acttgctcgt agccctggtc tctctcgtct tcttcgacac tctcgatccg gtcatcactc
     3421 tccttgtcgt cctgggactc gggttggtgg tggttgcggt atccctatcc aaggggggcc
     3481 tcggccgaga tgacttgctg tggatgatga tcggc
//
LOCUS       DASTXO010000558         2819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_550291, whole genome shotgun sequence.
ACCESSION   DASTXO010000558 DASTXO010000000
VERSION     DASTXO010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550291"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VFU96_11860"
     CDS             <1..281
                     /locus_tag="VFU96_11860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU5114005.1"
                     /translation="HSALPLDDFVEVAAPRGADHPEVAGEVLELRGRLRRALDSLPEP
                     QRQVVVLKDVYDWSHAEIAEAMGISVSAAKVRLHRARARLARQLEESA"
     gene            278..610
                     /locus_tag="VFU96_11865"
     CDS             278..610
                     /locus_tag="VFU96_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5114006.1"
                     /translation="MNVFCEVAAPFVANSAVEGSPVARPFRAHAEECLKCQARHAAMA
                     RTARELAAMSQETFNAPLDLEWRVMSALEGDLAVARSWRTPVAVSAAIVSMAAAVLIW
                     RLRPRTQT"
     gene            690..1184
                     /locus_tag="VFU96_11870"
     CDS             690..1184
                     /locus_tag="VFU96_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020635433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEU5114007.1"
                     /translation="MKALISTASKHGSTAEIGDVIGEVLREHGIEIDVLGPEAVTSLE
                     GYDLTVLGSAVYAGHWMKSMRELAERLAEDLAARPVWLFSSGPIGDPPKPDEDPVDVA
                     EVMERTGARGHAVFAGKIERSRLGFGERAIVSALKVPEGDFRDWDEIRRWAAGIVEEL
                     RLSV"
     gene            1200..1850
                     /locus_tag="VFU96_11875"
     CDS             1200..1850
                     /locus_tag="VFU96_11875"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017594834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEU5114008.1"
                     /translation="MTRLIDLSHTIEDGMITYPGLPGPVLSDHLSRVDSRANYEPGTE
                     FLIGRIDMVGNTGTYLDTPFHRFDGGADLAGLALDDVADLPGLCIDAPGPEIGPEVVD
                     GLDLAGRAVLFATGWDRHWGADAYGDPSHSFVSAPLVEALVDGGAVLVGIDSVNIDDT
                     RGGARPAHTGLLAAGIQVVEHLTGLGQLVGRDFRFYAVPPKVSGFGTFPVRAFAIT"
     gene            complement(1857..2333)
                     /locus_tag="VFU96_11880"
     CDS             complement(1857..2333)
                     /locus_tag="VFU96_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEU5114009.1"
                     /translation="MPDETKQSVRIPPRWFVRTAWVVHRALYRVTGGRFGLWPPTPDK
                     WGTMRLTTTGRRTGQQRTVILGYFEDGPNVITMAMNGWADDEPAWWLNLQANPMASVD
                     LVDGARTVRGRAAKGEERSRLWDRWLQIDSRLDDLAALRSGETAVVILEPWTDAGP"
     gene            complement(2355..2750)
                     /locus_tag="VFU96_11885"
     CDS             complement(2355..2750)
                     /locus_tag="VFU96_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018252493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5114010.1"
                     /translation="MPVLELVTIVVDDYDEAIEFFVGVLGFALVDDSPSLTNDGRPKR
                     WVVVRPHGGETGILLAHADGEDQGQTVGNQVAGRVGFFLRVDDFEAAHDRMVAAGVEF
                     VREPRNEDYGRVAVFLDVCGNRWDLLGPV"
BASE COUNT          475 a          902 c          925 g          517 t
ORIGIN      
        1 ggcacagcgc gctgccgctc gacgacttcg tcgaggtcgc ggcgccgaga ggggccgatc
       61 acccggaagt cgccggggag gtcctcgagc tgcgcggccg gctgcgtcgc gccctggact
      121 cactaccgga accacaacgt caggtcgtcg tcctcaagga cgtctatgac tggtcgcatg
      181 ccgagatcgc cgaggccatg gggatctcgg tgtcagccgc caaggtccgt ctccaccggg
      241 cacgggcacg tctcgcccgg cagttggagg agtctgcatg aacgttttct gcgaggtcgc
      301 tgcgccgttc gtcgccaaca gcgccgtcga aggctccccg gtcgcccgac ccttccgtgc
      361 ccacgccgaa gaatgcctca agtgccaggc gcgacacgcg gccatggccc gcaccgcccg
      421 tgagctggcc gcgatgtcgc aagagacgtt caatgcccca ctcgacctgg agtggcgggt
      481 gatgtcggcg ttggagggtg acctggcggt ggcccgctcc tggcgtaccc cggtggcggt
      541 ctccgccgcg atcgtgtcga tggccgccgc ggtcctcatc tggcggctcc gccctcgcac
      601 acagacctga tcgataggga ctttgttctc tggccggttc ggcgaatccg ccgtaatcca
      661 tatgtcaggt cgagagaaag gcagacctga tgaaggccct gatcagcacg gccagcaaac
      721 acggctcgac cgccgagatc ggcgacgtca tcggtgaggt cctccgcgaa catggcatcg
      781 agatcgatgt gctcggaccg gaggcggtga cctcgctaga gggttacgac ctgacggtcc
      841 tgggcagcgc ggtgtacgcg ggtcactgga tgaagtcgat gcgggagctg gcggaacggc
      901 ttgccgagga tctggcggcg cgcccggtgt ggttgttttc cagcggtccc ataggcgacc
      961 ctccgaagcc ggatgaagac ccggtggacg tggccgaagt catggagcga accggtgccc
     1021 gtggtcacgc agtatttgca ggaaagatcg aaagatcccg gctcggattc ggcgagcgcg
     1081 ccatcgtgag tgcgctcaag gtccccgagg gcgatttccg ggactgggat gagatccgca
     1141 gatgggcggc cgggatcgtc gaggagctcc ggcttagcgt gtgaggatac gctgggacga
     1201 tgacgcgtct catcgacctc tcccacacaa tcgaagatgg gatgatcacc tatcccggtc
     1261 ttcccgggcc tgtcttgtcg gaccatctct cccgggtcga ctcccgggcg aactacgagc
     1321 ccggcacgga gttcctcatt gggcgaatcg acatggtcgg gaacaccggc acatatctcg
     1381 atacgccttt ccatcgattc gacggtggag ccgatctcgc cgggctggca ctcgacgacg
     1441 ttgccgactt gcccgggctc tgtatcgacg cacccggtcc ggagatcggg ccggaggtgg
     1501 tggacggcct ggacttggcc ggccgggctg tcttgtttgc caccggatgg gatcggcatt
     1561 ggggcgccga cgcctacggt gaccccagcc attcatttgt gtcggcccct cttgtcgagg
     1621 cactcgtcga tgggggtgcc gtgttggtcg gcatcgactc ggtgaacatc gacgacacgc
     1681 ggggcggggc caggccggcg cacaccggtc tactcgccgc cggcatccag gtcgtcgagc
     1741 atctgaccgg tctagggcaa ctcgtgggcc gggatttccg gttctacgcg gtgccaccca
     1801 aggtgagtgg attcgggacg ttcccggttc gggcctttgc gatcacatga tgaacgctat
     1861 ggaccggcgt cggtccaagg ttcgaggatg acgaccgcgg tctcgccgga tcggagggcc
     1921 gccaggtcgt ccaatctcga gtcgatctga agccatcgat cccacaagcg tgaacgctct
     1981 tcccctttgg ctgctcgacc cctcaccgtc cgtgcgccgt ccacgaggtc gacgctggcc
     2041 atggggttgg cctggaggtt gagccaccat gccggctcgt cgtcggccca tccgttcatc
     2101 gccatggtga tcacgttcgg tccgtcttcg aagtagccga ggatcacggt ccgctgctgg
     2161 ccggttctgc gcccggtggt ggtgaggcgc atggtgcccc atttgtcggg cgttgggggc
     2221 cagaggccga atcggccgcc agttacccgg tacagggctc gatggacaac ccaggcggtg
     2281 cgaacgaacc agcgaggggg aattctgacg ctttgcttgg tttcatctgg catgaggtcg
     2341 tgcactcatg gtcatcaaac aggaccgagc aggtcccagc ggttgccgca gacatcgagg
     2401 aagacggcga cgcgcccata gtcttcgttg cgtggttccc tgacgaactc gaccccggcg
     2461 gccaccatcc gatcgtgggc tgcctcgaag tcatcgaccc gaaggaagaa cccgacgcgt
     2521 ccggcaacct ggttccccac ggtttgtccc tgatcctcgc cgtcggcatg ggcgagcaga
     2581 atccccgtct ctcccccatg aggtcgaacc accacccacc gtttgggccg gccatcattc
     2641 gtcagcgaag gcgaatcgtc cacgagcgcg aatccgagga caccgacgaa aaactcgatg
     2701 gcctcgtcgt aatcgtcgac gacaatggtg accagttcca acaccggcat cgaagaagtc
     2761 tgccatgaga cccggagttt ccacaccacc cgccttccct tatactttgt catcccgcg
//
LOCUS       DASTXO010000559         4054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1397212, whole genome shotgun sequence.
ACCESSION   DASTXO010000559 DASTXO010000000
VERSION     DASTXO010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397212"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1974)
                     /locus_tag="VFU96_11890"
     CDS             complement(139..1974)
                     /locus_tag="VFU96_11890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEU5114011.1"
                     /translation="MRLRRTIALFAASLTLLGIAAPVLAADEEWEFDGGGWGHGVGLS
                     QFGALGQAQDGRNSTQILQHYYTGTSVASMPGNHWTRGANGLWVGLVSNTNSVALTAV
                     GGALGICQPAGQCPPLPPYSSGFTDLTINPGEAWVFERNPLNHNQCRFRKKDAGNTGW
                     TACSGQVTGLSPSTRVSVNGTQYARGAIRFVDSSAGFHVVVTLGLEQYLYGLAEVPSS
                     WPTHALRAQAIIGRSYAVATAVQRGGADGATKLSSCGCHLFSTTADQAYVGWSKENPT
                     AANFGSQWKAAVDDTSARVLTHPQSDYALHIAKAFYSSSNGGASEDVEFVWGGTALPW
                     LRSVSDKWSSDPSVNPLATWTVVVPAPRIEQAFGWSAVTKVEKISGPPGAVVRFTGVR
                     GGSNVTREMWGDQLRIFLIDNAYRRDGAEVRVSPYISGIGYRGPFLDIANNTFESAII
                     WLAQQKITLGCNPPLNSNFCPDKEVTRGQMAVFISRALNLPGAGSDHFADDDGKFYEA
                     AANQLYESGITFGCRANSFCGDQPIPRGQMAAFLARAYELPGAATDHFKDDSKSEFQA
                     AINKIASAGITLGCNPPTNDRFCPGDRVTRGQMAAFLKRATEWDT"
     gene            complement(2017..3708)
                     /locus_tag="VFU96_11895"
     CDS             complement(2017..3708)
                     /locus_tag="VFU96_11895"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEU5114012.1"
                     /translation="MHLAAFALALSMFQVVGLPPGGTFTDDDGHVLEGGIEAIAGERI
                     TIGCNPPFNDRYCPERVLTRAQMAAMVARARNLPQTTTDAFIDDNGHVLEPAINRLAA
                     AGVTFGCNPPANDRFCPDDTVTRAQAAGFLARALSIPPSGTNHFIDDNGHVLEGAINR
                     IADRGITLGCNPPTNNRFCPNDGVTRAQIAGMLTRALGLTPMRPPARPALSWATVVDG
                     LEAPIQVLAPPGENRLLIAQQGGVVRIAQGGTLTGVFLDISDDVIFSGERGLLSIALP
                     PDYPADRRLFAWYSGALRSGSSGDHTTYLVEYDIAPNLQTASSPRIVLAVDQPFSNHN
                     GGFLSFGPDGHLYLSLGDGGSGNDPGGRARNLNTLLGKMIRIDVDGAQPYAIPADNPY
                     VGRSGRDEIWASGLRNPFRWSFDGNLMFIGDVGQGSREEIDAVPVEPRGYDFGWSRFE
                     GTVCNPNDTDPSCSRSGLTFPIEEYGRSVGRTVTGGVVYRGPTVRSLDKYYVYADFGS
                     GRVLAFRYLNGRAVESRDLTGRLGNTGFVSFEIDNNGEMLAVGYLDGAVYRLRGG"
     gene            3778..>4054
                     /locus_tag="VFU96_11900"
     CDS             3778..>4054
                     /locus_tag="VFU96_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5114013.1"
                     /translation="MTPVLALLMVVGMTPAAAPAPEPVETIQVAIPAEVASDNALVQS
                     EAGKGRAAKGEQWVSVEVPVQGTPAATLRSLSRELGVQVWEDHVLRLQ"
BASE COUNT          776 a         1256 c         1294 g          728 t
ORIGIN      
        1 ccgatcgagg gaagcccacg gctcgccgcg gtgacgagca ggggcaaccc cacgtaaccc
       61 agcccgatga ccccagccgt ccatgcctgg ttggagaaga agtcgatcct gtccgtcacc
      121 gccgcgatgg taggaggatc aggtatccca ctccgttgct cgtttgagaa atgctgccat
      181 ctgtccccgg gtcacgcggt cgcccgggca gaatctgtcg ttggtcggtg gattgcagcc
      241 aagtgtgatg ccggcggacg cgatcttgtt gatcgcggcc tggaattctg atttcgagtc
      301 gtctttgaag tggtcagtgg cggcgccagg cagctcgtag gcccgtgcca ggaaggcagc
      361 catctggcct ctcggtattg gctggtcgcc acagaagctg ttggcccgac aaccgaaggt
      421 gattccggac tcgtagagct ggttggccgc ggcctcgtag aacttcccgt catcgtctgc
      481 gaaatgatct gagcccgccc ccggcagatt caatgccctg gagatgaaca ccgccatttg
      541 gcccctggtc acttccttgt cggggcagaa gttgctgttc aagggtgggt tgcaaccaag
      601 cgttatcttc tgctgggcga gccagatgat ggccgattcg aaggtgttgt tggcgatgtc
      661 gaggaagggc cctcgatagc cgatgccgct gatgtagggc gacacccgca cctcggcgcc
      721 gtctctccga taagcattgt cgatgaggaa gatcctcagc tggtcacccc acatttcgcg
      781 agtgacgttc gaaccgccac gaacgcctgt gaatcggacc acggcccctg gtggtccgct
      841 gatcttctcg accttggtga cggccgacca gccaaaggcc tgctcgatcc tcggggcggg
      901 aacaaccacg gtccaggtgg cgagcggatt caccgacggg tcggacgacc atttgtcgga
      961 aaccgatcgc aaccagggaa gggccgtccc tccccacacg aattccacgt cttcactcgc
     1021 tccgccgttg gaagaggagt agaacgcctt cgcgatgtga agggcgtaat cggattgggg
     1081 gtgcgtcagc accctcgcgc tggtgtcatc gacagcggct ttccactgcg acccaaagtt
     1141 cgccgcggtc gggttctcct tgctccaacc gacgtatgcc tgatcagccg tagtcgagaa
     1201 cagatgacag ccgcagctag acagcttggt ggccccgtcc gcgccaccac gttggaccgc
     1261 agtggctacc gcataactgc ggccgatgat cgcctgcgcc cgcagcgcat gggttggcca
     1321 agacgagggg acctcggcaa gaccgtagag atactgctcg agcccgagag tgacaacgac
     1381 gtggaaacca gcgctggagt cgacgaaccg gatggctcca cgggcgtact gcgtgccatt
     1441 caccgagacc ctggtcgatg ggctgagacc cgtgacctgg ccactgcagg cagtccatcc
     1501 ggtgtttccg gcgtccttct tgcggaagcg acactgattg tgattgaggg ggtttcgctc
     1561 gaatacccaa gcctctcccg ggttgatcgt gaggtcggtg aaaccagatg aatagggggg
     1621 tagcggagga cactgccccg ccggctggca gatcccgagg gcgcctccca cagcggtgag
     1681 ggcaaccgag ttcgtgttcg agacaaggcc gacccagaga ccgttcgcac ctctggtcca
     1741 atgattgccc ggcatggagg caaccgaggt tcccgtgtaa tagtgctgga gtatctgagt
     1801 cgagttgcgg ccgtcctgcg cctggccaag cgcaccgaat tgggacagac cgactccgtg
     1861 accccaacca ccgccatcga actcccactc ctcgtcagca gcaaggacag gcgcagcaat
     1921 gcccagaagc gtcagggacg cggcaaagag agcgatcgtg cgtctcagcc tcacaccgcg
     1981 tgaatcggcc cgtgcttggc cagcctgaag agaatctcaa ccaccccgga gccgatagac
     2041 ggcaccgtcg agatacccga cagcgagcat ttcgccgttg ttgtcgatct cgaacgagac
     2101 gaagccggta ttgcccagcc tgccggtgag gtcgcgtgac tcgacggcgc gtccattcag
     2161 atagcgaaag gcgagcaccc ttccgctccc aaagtcggca tagacgtagt acttgtcgag
     2221 cgatcgcacc gtcggtccgc ggtacaccac tcccccggtc acagtccggc cgaccgacct
     2281 cccgtattcc tcgatcggga aggtcagacc cgatcgcgag caactgggat cggtgtcgtt
     2341 cggattgcag accgtcccct cgaatcggga ccacccgaag tcgtagcccc tgggctccac
     2401 tggcacagcg tcgatctcct cccgtgaccc ctggccgaca tcaccgatga acatcaggtt
     2461 gccgtcgaac gaccagcgga acgggttgcg cagcccggag gcccatatct cgtcacgtcc
     2521 cgatctcccg acataggggt tgtcggccgg aatggcatag ggctgagctc cgtcgacgtc
     2581 gatccggatc atcttgccga ggagggtgtt cagattccgg gcccgaccgc cgggatcgtt
     2641 tccgctcccg ccgtcaccca gagacagata gagatgacca tcggggccaa aggagagaaa
     2701 accgccgttg tggttgctga aaggctgatc gacggcgagg acgatcctcg gggaggaagc
     2761 agtctgcaga ttgggggcga tgtcgtactc gaccaggtac gtggtgtggt ctccgctcga
     2821 acccgatcgg agcgcgcccg aataccaggc gaagagacga cgatcggccg ggtagtccgg
     2881 ggggagtgcg atcgagagaa ggccccgttc cccgctgaat atgacgtcat cggagatgtc
     2941 gaggaagacc ccggtcaggg tgccaccctg cgcgatccgg accacgccac cctgctgggc
     3001 gatgagcaac cggttctccc ccggcggggc aagcacctga atcggcgctt ccaacccgtc
     3061 gaccaccgtt gcccagctga gggcaggccg cgccggtggt cgcatcggag tcagaccaag
     3121 ggccctggtc aacatcccgg ctatctgggc acgggtgaca ccgtcgttgg ggcagaagcg
     3181 gttgttggtc ggggggttac agcccagcgt tatgccgcga tcggcgatgc ggttgatggc
     3241 cccttcgaga acgtgtccgt tgtcatcgat gaaatgattg gtgcccgacg gagggattga
     3301 tagcgctcga gcgagaaatc ccgccgcctg ggcgcgggtg accgtgtcgt cggggcagaa
     3361 gcggtcattg gcgggagggt tgcatccgaa ggtgacccct gcggcggcaa gtcgattgat
     3421 cgcaggctca agaacatggc cgttgtcgtc gatgaacgca tcggtggtgg tctggggcag
     3481 attgcgcgct ctcgcaacca tggctgccat ctgagctcga gtcagcaccc tttccgggca
     3541 gtaacgatcg ttgaatggag ggttgcaacc aatcgtgatt cgctcgcccg ctatcgcctc
     3601 gattccaccc tcgagcacat gcccgtcgtc gtcggtgaag gtaccgccag ggggcaggcc
     3661 cactacctgg aacatgctca gagccagtgc gaaggcagca agatgcatca tcaagcgcgg
     3721 accaaggtac ccgctcccgc tccctaacca accaggtctg tgatcatgag catctcgatg
     3781 acccccgttc tagccctctt gatggtggtc gggatgaccc cggccgccgc cccggctcca
     3841 gagcccgtgg agacgattca ggtcgcgatt ccggccgagg tcgcctcgga taacgcactc
     3901 gtccaatccg aggccggcaa agggcgagca gcgaagggcg agcaatgggt cagtgtcgag
     3961 gtgccggtcc aggggacccc ggctgcaacg ctccgttcgc tgtcccgtga gctgggtgtt
     4021 caggtctggg aagaccacgt tctccggctc caag
//
LOCUS       DASTXO010000560         3505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_991737, whole genome shotgun sequence.
ACCESSION   DASTXO010000560 DASTXO010000000
VERSION     DASTXO010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991737"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VFU96_11905"
     CDS             <1..803
                     /locus_tag="VFU96_11905"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEU5114014.1"
                     /translation="TMIQADEALDVLDSALVAVAGLQAGLVRKYRGQPQIGRTFLQHA
                     RPTTFGMTVAGWLEPTLRHIDELRHVKSGLAVQLGGPVGDLAAYEGKGIAVVEALADR
                     LRLTAPTLPWHADRSRISALASALARCARTMAKVGLDIALLASSEIAEITVRPGGSSS
                     MGEKRNPIDSLRAMAAAELATAAAGMITGGRLSELDRGIGGWHAEWVAVPLVFQATAA
                     TVEAMRTCLGSVDVAAETMSARVADSPQIDQALIEGVLAEFERVVGRR"
     gene            800..1588
                     /gene="pcaD"
                     /locus_tag="VFU96_11910"
     CDS             800..1588
                     /gene="pcaD"
                     /locus_tag="VFU96_11910"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR02427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HEU5114015.1"
                     /translation="MTTGRTGTLTWYTVGDDSAQPLVMIHALGADSEMWEAQLPRLST
                     MRRVVMVDLPGHGNSVAGPGPYTIRSLGEDILDVATTAGIHEFDLCGMSLGGVISLWM
                     AAHSPDRVMSLVASNTSARVGSEEIWQARIDAVTEGGVEAISEQALARFFTSSFASEQ
                     PDVYVRTSRILLDTDPAGYAACCAALRDADLRELVEAIRCPTLVIGGGEDISTPPEQS
                     IWLQEHIPGSRLHILEGAPHFSNLEQPEAWTTLVTEFLVEQQAT"
     gene            complement(1585..2376)
                     /locus_tag="VFU96_11915"
     CDS             complement(1585..2376)
                     /locus_tag="VFU96_11915"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEU5114016.1"
                     /translation="MVETAREEGTFTPDEMMTVTASRGLRNGQVCFVGIGLPSEAANL
                     ARLSHAPDVVLIYESGTIGTRPEILPLSIGDGELAEKADLVVSVPEIFNYWLQGGRID
                     VGFLSAAQIDRYGNLNSTVIGSYEHPVVRLPGAGGAPEIASSAGKVIVMLRQNPRSFV
                     DAVDFVTSVGFGRHGEGRQGNVGAGPTVVITDLGVLRPDPVTNELTLVSVHPGVTVEQ
                     AVAATGWDLVVRESLEVVEPPTTAELATLRALKARSSDAHARTGG"
     gene            complement(2369..3223)
                     /locus_tag="VFU96_11920"
     CDS             complement(2369..3223)
                     /locus_tag="VFU96_11920"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEU5114017.1"
                     /translation="MAEIVSLADAVADLVPDGATVAMEGFTHLIPFAAGHELIRQERK
                     DLTLARMTPDVIYDQLIGMGCARGLIFSWGGNPGVGSLHRLRDAVEHGHPRPLELEEH
                     SHAGMAARYAAGASGLPFGVLRGYAGSDLLAHTTVARISCPFTGEELAAVPALTPDVA
                     VIHAQQADRKGNVGLWGITGVQREAVLAARSSIVTVEEIVDELSETGPNHVVLPAWTV
                     TAVAHVPRGSHPSYSMGFTTRDNAFYTDWDAISRDRDSFSRWMNEFVVGSPDHAAYLD
                     LISSRAHG"
     gene            3314..>3505
                     /locus_tag="VFU96_11925"
     CDS             3314..>3505
                     /locus_tag="VFU96_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5114018.1"
                     /translation="MAGQSFHHAAMTSLESSQVWESLDEPRTWEAIPGVDRVFDPSVD
                     LDGHLRGFSFESDVGGRTYV"
BASE COUNT          607 a         1167 c         1140 g          591 t
ORIGIN      
        1 ccaccatgat ccaggccgac gaggcactcg atgtcctcga ctctgccctg gtcgcagtcg
       61 ctggcctgca ggccgggctg gtccgaaagt atcgcggtca acctcagatc gggaggacct
      121 ttctccagca cgcccgaccc acgactttcg ggatgaccgt ggcgggctgg ctcgagccca
      181 ccctgcgaca catcgacgag ctcaggcatg tgaaatcggg cctggcggtc cagctcggtg
      241 gccccgtcgg ggatctcgcc gcgtacgaag gcaagggtat agccgtggtg gaagccctcg
      301 ccgaccggct ccggctcacc gctcccaccc ttccctggca cgccgatcgg tctcgcatct
      361 cggcgctggc ctcggctttg gcgcgatgcg ctcgcacgat ggcaaaggtg ggtctcgaca
      421 tcgccttgct ggcgtcgagc gagatcgccg agatcacagt tcggccgggc ggctcttcca
      481 gcatgggcga gaagcgcaac ccgatcgatt cgttgcgggc catggcggcc gctgagctgg
      541 ccacggccgc ggccggcatg atcaccggcg gtcgactctc ggagctggat cggggtatcg
      601 gtggctggca tgccgagtgg gtagcggtgc ccctggtctt ccaagcgacg gccgcgacgg
      661 tggaggcgat gaggacctgt cttggctccg tcgacgtggc ggccgagacc atgtccgccc
      721 gggtcgccga cagccctcag atcgaccagg ccctgatcga gggagtcctc gccgagttcg
      781 agcgcgtcgt gggtcgccga tgaccacggg gcgcaccggg actctgacct ggtacacggt
      841 cggggacgat tcggcgcaac cgctggtcat gatccacgcc ctgggcgccg acagtgagat
      901 gtgggaggcc cagctccccc ggctctcgac gatgaggaga gtggtgatgg tggatctccc
      961 cgggcacggc aactcggtgg ccgggcccgg gccctacacc atccggtctc tgggcgagga
     1021 cattctcgac gtggccacca ccgcgggaat ccacgaattc gacctctgcg gcatgtcgtt
     1081 gggtggtgtc atctcgctgt ggatggcagc ccactcaccg gaccgcgtga tgagcctggt
     1141 cgcctcgaac acctcggcca gagtcgggag cgaagagatc tggcaggcca ggatcgacgc
     1201 ggtcaccgag ggcggcgtgg aggcgatctc ggagcaggct ctcgccaggt tcttcacgtc
     1261 gagctttgcc tccgagcagc ctgacgttta cgtccgcacc tctcgaatcc tgctcgacac
     1321 cgaccccgcc ggatacgccg cctgctgcgc cgcactgcga gacgccgacc ttcgggagct
     1381 ggtggaggca atccgttgcc cgaccctggt gatcggtggg ggcgaggaca tctccacgcc
     1441 gccagagcag tcgatctggc tgcaagagca catcccaggc agcaggctcc acatcctgga
     1501 gggcgccccc catttctcca atctcgagca accggaggcg tggacgacgt tggtcacgga
     1561 attcctcgtg gagcagcagg ccacctagcc cccagtccgg gcatgcgcat cgctgctacg
     1621 cgccttgagg gctcgcagtg tggcgagctc cgccgtggtc ggcggctcaa ccacttcgag
     1681 ggattctctc accacgagat cccagccagt ggcggcgacc gcttgctcga ccgtgacccc
     1741 cgggtgaacg gacacgaggg tcaactcatt cgtgaccgga tccggtcgaa ggactcccag
     1801 atcggtgatg accaccgttg gcccggcccc tacgttcccc tggcggccct cgccatgacg
     1861 cccaaaaccg accgaggtga cgaaatcgac cgcgtcgacg aacgagcgcg gattctgacg
     1921 gagcatcacg atgactttcc ccgccgaaga ggcgatctcg ggagcgccgc cggccccggg
     1981 cagcctcaca acggggtgct cataggaccc gatcaccgtg ctgttgagat tcccataacg
     2041 gtcgatctgc gcagccgaga gaaaaccgac gtcgatccga ccaccttgga gccaatagtt
     2101 gaagatctcg gggacgctga ccaccaggtc ggccttctca gccagttcgc cgtcgccgat
     2161 ggagagggga aggatctcgg ggcgagtgcc aatggtgccc gactcgtaga tcagaaccac
     2221 gtcgggagca tgcgacagtc gggccaggtt tgccgcctcg ctggggagac ctatcccgac
     2281 gaagcacacc tgtccgttcc tgaggcctcg ggacgcagtc accgtcatca tctcatcggg
     2341 cgtgaaggtt ccttcctccc gagcggtctc aaccatgggc acggctcgag atcagatcca
     2401 ggtatgcggc gtggtcgggc gagcccacca cgaactcgtt catccatctc gagaatgagt
     2461 cccggtcccg ggagatggcg tcccaatcgg tgtagaaggc attgtcccga gttgtgaatc
     2521 ccatcgagta cgacgggtgt gagccacgcg gaacgtgcgc cacggcagtg acggtccacg
     2581 cgggcaacac gacgtgattc ggccccgtct ccgagagctc atcgacgatc tcctcgacgg
     2641 tcacgatcga agacctggcg gccaataccg cctcgcgctg gacgccggtg atcccccata
     2701 ggccgacgtt tccctttcga tcggcctgct gggcgtgaat gactgcgaca tccggtgtca
     2761 gtgcgggaac cgccgccagc tcctccccgg tgaacgggca cgagatgcga gcgaccgtgg
     2821 tgtgggcgag gaggtctgac ccggcgtatc cccggaggac gccaaagggg agaccggagg
     2881 cgccggctgc gtaccgcgcc gccatgccgg cgtgactgtg ctcctccagc tccagtggcc
     2941 ggggatgacc atgctcgacc gcgtcccgaa gtctgtgcaa cgaaccgaca cccggattcc
     3001 cgccccagga gaagatcagg ccccgggcgc aacccatgcc gatcaactgg tcgtagatca
     3061 cgtcgggagt catgcgtgcc agcgtcaagt ccttgcgctc ctgcctgatc aactcgtgac
     3121 cggcggcaaa tgggatcaga tgggtgaatc cctccatggc caccgtcgcc ccgtccggga
     3181 ccaggtcggc gacggcgtcg gcaagcgaca cgatctcggc catcgctaat tcctatgggg
     3241 ccaagcagac aactcagcca cgataccgcc ggccgattca ctcgcttcgc tacccgatca
     3301 ttagcttgcc ggcatggccg ggcagagctt tcaccatgca gcgatgacgt cgttggagtc
     3361 ttctcaggtc tgggagtcgc tcgacgagcc tcgcacctgg gaggcaatcc cgggagtgga
     3421 tcgggtcttc gaccccagcg tggatctgga tggccatctc cgtggcttct ctttcgagag
     3481 cgacgtcggc ggacggacct atgtc
//
LOCUS       DASTXO010000561         2077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1102377, whole genome shotgun sequence.
ACCESSION   DASTXO010000561 DASTXO010000000
VERSION     DASTXO010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102377"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="VFU96_11930"
     CDS             complement(<1..1062)
                     /locus_tag="VFU96_11930"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEU5114019.1"
                     /translation="MGEVQVPADAYYGASTQRAVENFPISGNRFGRRFIWSLGLIKGS
                     AAEIAGRQAHLDQDKADAVRIAADEVMDGRLDHQFVLDIFQTGSGTSTNTNANEVIAG
                     RATEILGGETGSSLVHPNDDVNFGQSSNDVIPTAIHVAAVASIREDLIPSLAGLAGSL
                     EAKAAEFENVVKSGRTHLMDATPVTLGQEFGGWAAQVRKGIRRLEKVMPELEELALGG
                     TAVGTGINAPSGFAGAVIELMAQRTGMPFTEAVDHFEAQGSKDAVVMASGALKTIAVS
                     LFKIANDIRFLGSGPTSGIGEIRIPELQPGSSIMPGKVNPVMSEMVMQVAAQVVGNDA
                     TVTWSGANGNFELNVMMPVM"
     gene            complement(1134..>2077)
                     /locus_tag="VFU96_11935"
     CDS             complement(1134..>2077)
                     /locus_tag="VFU96_11935"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5114020.1"
                     /translation="DSGGNLLITRKGKKRRDPILAVAHMDHPAFVVTSVTGREAVFEF
                     RGGMRSEYFPDARVDFAPGRAKDGRVASYDPESRTGVIQVNGALPAPGDIARWHMADP
                     RPRADRLMAPACDDLAGAAAALAALDKARATADLAHFGVLLTRAEEMGFVGAIHAAVT
                     GSIPADARLLSIEASRASVDAPLGKGPVIRVGDAVTVFDRELTNRISMAVAASGLRHQ
                     RKLMVGGACEATAFGVYGYAATGLCLPLGNYHNMGDLDAVESGNGKARPMLEEVSLDD
                     FHDMVDLLLVAAAAVDDHGDLRERLDRVYSDGRHVLG"
BASE COUNT          364 a          698 c          632 g          383 t
ORIGIN      
        1 catgaccggc atcatcacgt tcagctcgaa gttgccgttc gccccgctcc aggtgacggt
       61 tgcatcgttt cccaccacct gagccgcaac ctgcatcacc atctccgaca tgaccgggtt
      121 cactttgcct ggcatgatcg atgacccggg ctgcagttcg gggatcctga tctcgccgat
      181 gcccgaggtg ggcccggagc caaggaagcg gatgtcgttg gcgatcttga acagagacac
      241 tgcgatcgtc ttgagggcgc ccgaggccat caccacggcg tccttcgacc cttgagcctc
      301 gaaatggtcg acggcttcgg tgaatggcat tccggtgcgc tgcgccatca gctcgatgac
      361 agccccggcg aaccccgagg gcgcgttgat ccccgtgccg acggcagtcc cgcccaacgc
      421 cagttcctcc aactccggca tgaccttctc cagccgccgg atcccctttc gcacttgggc
      481 agcccaaccg ccgaactcct ggccgagagt gaccggggtg gcgtccatga ggtgggtccg
      541 acccgatttc acgacgttct cgaactcggc ggccttggcc tcgagcgatc cggcgagccc
      601 ggccagggag ggaatcaggt cttctctgat cgacgccacg gcggcgacgt ggattgcggt
      661 ggggatgaca tcgttcgacg actgaccgaa gttcacgtca tcattcgggt gaaccagact
      721 cgaaccggtc tctcccccca agatctccgt cgctcgaccc gcaatcacct cgttggcgtt
      781 cgtgttggtc gatgtgccgg aaccggtctg gaagatgtcc agaacgaact ggtggtcgag
      841 gcgcccgtcc atcacctcat ctgcggcgat ccgcactgcg tcggccttgt cctggtcgag
      901 atgagcctgc cgacctgcga tctcggctgc cgagcctttg atcagaccga gcgaccagat
      961 gaatctccgg ccgaatcggt ttcctgagat cgggaagttc tcgacggcgc gttgagtgga
     1021 cgctccgtaa taggcatcag cggggacttg cacctcaccc atcgagtcct tttcgaccct
     1081 gtattccact catctctcct tgccacaacg acggtagtgg gcgacggtca tcgtcacccg
     1141 agaacgtggc ggccgtcgga atacaccctg tcgagacgtt cccgcaagtc gccatggtcg
     1201 tccacggcag cggcggcgac gagcaggaga tcgaccatgt cgtggaagtc gtccagcgag
     1261 acctcctcca acatcgggcg ggctttcccg tttccggact ccactgcatc gagatcgccc
     1321 atgttgtggt agttcccgag aggaaggcaa agaccggtgg cggcgtaccc gtagacccca
     1381 aaagctgttg cctcacaggc gcctcccacc atcagcttcc gctgatgacg gagaccgctc
     1441 gccgcaaccg ccatgctgat ccggttcgtg agctcgcgat cgaacacggt gaccgcgtca
     1501 ccgacccgga tcaccgggcc tttgcccaac ggagcgtcaa ccgatgcccg ggaggcttcg
     1561 atggagagca gccgggcatc agcaggaatg cttccggtga ccgcggcgtg gatggcgccc
     1621 acgaagccca tctcctcggc ccgggtcagc aggacgccga aatgcgccag atcggcagtg
     1681 gctcgcgcct tgtccagagc ggccagcgcc gctgctgccc cggccaggtc gtcgcaggct
     1741 ggtgccatga gacggtcagc tcgtggtctc ggatcagcca tgtgccagcg ggcgatatcc
     1801 cccggagcgg gcagagcgcc attcacttgg atgacgcctg tccggctctc cgggtcgtag
     1861 gaggcaaccc gcccgtcctt cgctcgaccg ggtgcgaaat cgaccctggc gtccggaaag
     1921 tactcggacc tcatcccgcc tcgaaactcg aagaccgcct cgcgaccggt caccgaggtg
     1981 acgacgaaag ccgggtgatc catgtgagcc acggccagga tcgggtcacg tcgcttcttt
     2041 cccttccggg tgatgaggag gttgcctcct gagtcct
//
LOCUS       DASTXO010000562         3905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1361116, whole genome shotgun sequence.
ACCESSION   DASTXO010000562 DASTXO010000000
VERSION     DASTXO010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361116"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VFU96_11940"
     CDS             <1..837
                     /locus_tag="VFU96_11940"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5114021.1"
                     /translation="IGARGQMFIGGLFALWVGLHVELPAFLHIPLALVAAIIGGGLWG
                     GLAGFLKARTGAHEVITTIMLNFIAAFFVLYVLKTSLFQEQGSNLPQSAPILESARLP
                     TLFGAQYRINISFLIALAAVFFVEWLLFRSTIGFEFRAAGFSPSASAYAGMNVAFLYT
                     TVMFISGGLAGLAGATMTLGLPPYTVTANFPGTIGFDAIALALLGRSHPYGVLWSGLL
                     FGALIAGGRTMQAAAGVSVDLVIVMQALIIIFIAAPKLVRAIFRLKPTEVEPTQLTKG
                     WG"
     gene            837..2120
                     /locus_tag="VFU96_11945"
     CDS             837..2120
                     /locus_tag="VFU96_11945"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5114022.1"
                     /translation="MTATTIERPEEVENRNRATPEERRSTIVGWVLIALGLFVIVAFG
                     FGAEGGADATFNLSLQTDRFQGLAWTVNARALSIVLGLLIVGLGVWRLTRRALEWTNI
                     ALAVGFAFFAMAFLAWAAAGSQFSLVGMLRSTVILSVVVVFGALTGLMCERVGVINIG
                     IEGQLLASAFAGVVLSSAFGTWAGLAGALLTGGLLGLLLAWLTIKFHVDQIIAGVVIN
                     FLALGLTSLMSSRILTDVQTLNAPKRVPSIRIPFLADIPVIGNMFFDHTFFVYGMFIL
                     VIAIHLGLFYTRWGLRSRSVGEHPMAADTAGIHVYWVRYRNVALGGMIAGFGGAFLGI
                     AQVARFEEDMTGGIGFIGLAAMIFGRWLPFGALGAGLVFGFARAVQQKVAILNIGIPS
                     EFLLMLPYVVTIIVVAGVVGKTRPPAADGQPYIKQ"
     gene            complement(2134..3132)
                     /locus_tag="VFU96_11950"
     CDS             complement(2134..3132)
                     /locus_tag="VFU96_11950"
                     /inference="COORDINATES: protein motif:HMM:NF024645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEU5114023.1"
                     /translation="MASRKCDQCGASVAADEQFCPNCGSFLDPLEPDAPRGRENVISV
                     SSDGNYEEFELGAPPPAAERSNPGRGEVTCPSCGANNPANNRHCQECGARLSQGPLPT
                     APRPAVQATAGVRAALAISALLFGVVVVALLFNVFSGDPEATASSTTVVSNTSTTIAE
                     LAPIDILDVECNQEGVGSFVCDNLIDGTETEYQFDYEAVPEGEAITIRLIFDRPTVVQ
                     RIDWVNLEDATRLRRNYRARGIVIAADNSLQPVLVQLEDLAGSQPVPFAALDATSIEI
                     TIESDYPAEVVEEERFTEMAIQEIRVIGRPGSITNGTNGATPTTTPGTGATTTAAS"
     gene            3477..>3905
                     /locus_tag="VFU96_11955"
     CDS             3477..>3905
                     /locus_tag="VFU96_11955"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5114024.1"
                     /translation="MTGWGEIDRVLGGGVVAGSVLLLGGEPGVGKSTLLLQLAGSLAG
                     RGDSVMVASAEESAEQVAMRASRLGIVHDAISLVSDDDVDAIVALAERKRPVLLMIDS
                     IQTVGVPEIGAAPGGVAQVREAAARLVRLAKTTGVAVVLVG"
BASE COUNT          605 a         1197 c         1280 g          823 t
ORIGIN      
        1 atcggagcgc gcggacagat gttcatcggt gggctcttcg ccctgtgggt cggcctccac
       61 gtcgagctgc cggcatttct ccatatccca ctcgctcttg ttgccgcgat catcggtggt
      121 ggcctgtggg gaggcttggc aggcttcctc aaggcgagga ccggcgccca cgaagtgatc
      181 accacgatca tgctcaactt catcgccgct ttcttcgtgt tgtacgtgct gaaaacgtct
      241 ctgttccagg agcaaggctc caacctgccc cagtcggctc ccatcctgga gtcggcccgg
      301 ctgcccaccc tgttcggcgc gcagtatcga atcaacatct cgttcctcat cgccctcgcc
      361 gcggtcttct tcgtggaatg gctcttgttc cggtcgacga tcggcttcga attccgcgcc
      421 gctggtttct cgcccagcgc cagcgcctat gccgggatga acgttgcgtt cctctacacg
      481 acggtgatgt tcatctccgg gggcctcgcc gggctcgctg gggcgaccat gactttgggg
      541 ctgcctcctt acaccgtgac tgccaacttc ccggggacga tcggcttcga tgcaatcgcc
      601 ctcgcccttc tggggcggtc acatccatat ggtgtgctct ggtcgggact tctcttcggg
      661 gcgctgatcg ccgggggtag gacgatgcag gcggcggccg gtgtgtccgt cgacctcgtc
      721 atcgtgatgc aggcactgat catcatcttc atcgccgccc ccaagctggt cagagcgatc
      781 ttccgtctga agccgaccga agtcgagccg acacaactga ccaagggttg gggctgatga
      841 cggctaccac catcgagagg cccgaagagg tcgagaacag gaatcgagcc acgccggaag
      901 agcggcgctc gacgattgtg ggctgggtcc tcatcgcgct cggattgttc gtgatcgtcg
      961 cgttcggatt tggtgcagag ggtggtgccg atgccacctt caatctgtct cttcagaccg
     1021 accgcttcca gggcctggcc tggacggtca acgcccgtgc tctctcgatc gtcctcgggt
     1081 tgctgatcgt ggggcttggt gtctggcgtc tgacccgccg ggccctggaa tggaccaata
     1141 tcgccctcgc cgtcggtttc gcattcttcg cgatggcatt cctcgcatgg gcagccgccg
     1201 ggagccagtt cagcttggtg ggaatgctcc ggtccaccgt gatcctctcc gtagtcgttg
     1261 tgttcggagc tctgaccggg ctcatgtgcg agcgggttgg ggtcatcaat atcgggatag
     1321 aaggccagct gctcgcctcg gcattcgccg gggtggtgct gagcagcgcc ttcggcacgt
     1381 gggccggtct ggcgggagcg ctactaacgg gaggcctgct ggggctgttg cttgcgtggc
     1441 tgacgatcaa gtttcatgtc gatcagatca tcgccggcgt ggtgatcaac tttctcgccc
     1501 tcggcctcac ctcgctcatg tcctcccgca tactcaccga cgtgcagaca ctcaacgcac
     1561 cgaaacgagt cccatcgata cgcatcccgt ttctggccga catcccggtc attggcaaca
     1621 tgttcttcga ccacacgttt ttcgtgtacg gcatgttcat actcgtcatt gcaatccacc
     1681 tcggcttgtt ctacacgcga tggggcctcc gctcgcgctc ggtcggagag catccgatgg
     1741 ctgctgacac ggctggcatc cacgtctact gggtgcgtta tcggaacgtg gccctgggtg
     1801 ggatgattgc ggggttcggc ggggcgttcc tggggattgc acaggttgcg cgcttcgagg
     1861 aggacatgac gggcgggatc ggattcatcg ggctcgccgc gatgatcttc ggtcggtggc
     1921 tcccgtttgg ggccctcggc gctggcctcg tgttcggatt tgcaagggca gtccagcaga
     1981 aggttgcgat cctcaacatc ggcataccgt ctgagttcct gctcatgctc ccctacgtcg
     2041 tcacgatcat cgtcgtcgcc ggggtggtgg ggaagacgag gcctcctgca gccgacggcc
     2101 agccttacat caagcagtag cggccggcgg ccgctacgag gcggctgtgg tggtggcccc
     2161 ggtgccgggg gtggtcgtcg gagtggctcc gttggtcccg ttggtgatgc tgccggggcg
     2221 gccgatgacc ctgatctcct gaatcgccat ctcggtgaag cgctcctctt caaccacttc
     2281 cgccgggtag tcgctctcga tagtgatctc gatcgaggtg gcgtccaatg cggcgaaggg
     2341 gaccggctgg ctcccggcca ggtcctcgag ttggaccagg accggctgaa gtgagttgtc
     2401 tgcggcaatc acgattccac gggcccggta gttgcgcctg agacgggtcg catcctccag
     2461 gttgacccag tcgatccgct gcacaacggt cgggcggtcg aagatgaggc ggatcgtgat
     2521 cgcctcacct tcgggcacgg cctcgtagtc gaactggtat tcggtctcgg tgccatcgat
     2581 caggttgtcg cagacgaagg agcccacacc ctcttggttg cactcgacgt cgaggatgtc
     2641 gatgggcgcc agttcggcga tcgttgtgct ggtgttggac accacggtcg tgctgctggc
     2701 ggtcgcctcc ggatcaccgc tgaagacgtt gaagagcaaa gcgacgacga cgacgccgaa
     2761 gagcagggca ctgatggcga gggcggcgcg cacgcccgcc gtcgcctgga ccgcaggacg
     2821 gggtgcggtc ggcaggggtc cctggctgag tcgtgcccca cactcctgac aatggcggtt
     2881 gttggccggg ttgttggccc cgcacgacgg acatgtgacc tcgccgcgac ccggattgga
     2941 gcgctcggca gcgggtgggg gagcgcccag ttcgaactcc tcgtaattgc cgtcggagct
     3001 caccgagatc acattttccc gacccctggg ggcatctggc tccaacgggt cgaggaacga
     3061 accgcaatta gggcagaact gttcgtcggc cgcgacactc gcgccgcact gatcacactt
     3121 ccgagaggcc acatccctcc ttcgtagtta ttgtgccacg ggatgtttct gtgggcgatt
     3181 agaacacatg agccctggac gatcgcctca atcctgacct cgacgatcgt tttcgcagca
     3241 cttgtggctg ccttgatttt cgccgttcag cggcgcaatc gttaaccgaa ataggaaaag
     3301 tcatggcatt tcgatgcgaa acctgcgggc acagatcggc caaatggatg ggtttctgcc
     3361 cacaatgcgg gtcgagcgag cctctggcgg aggtagccgg cgaagcccgc ccgaaggtca
     3421 gggcggccga ggccgttggc ctcacgactg ccgctggtca atcgaacgat cggaccatga
     3481 ctggttgggg ggagatcgac cgcgtattgg gcggtggagt ggtcgccgga tcggtgttgc
     3541 tcctgggcgg tgagccgggt gtcggcaagt cgacccttct cctccaattg gccggctccc
     3601 tcgccggcag aggcgattcg gtgatggtgg ccagcgccga ggaatcggcc gagcaggtcg
     3661 cgatgcgtgc cagccggctc gggatcgtgc acgacgccat cagcctggtc agcgatgacg
     3721 atgtcgacgc catcgtcgcc ctcgccgaac gaaagcgtcc cgtgctgctg atgatcgact
     3781 ccattcagac cgtgggagtg cccgagatcg gggctgcgcc cggtggtgtt gctcaagtcc
     3841 gggaagccgc ggcgcggctg gttcggttgg ccaagaccac cggtgttgcg gtcgtcctgg
     3901 tcggc
//
LOCUS       DASTXO010000563         5166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1287031, whole genome shotgun sequence.
ACCESSION   DASTXO010000563 DASTXO010000000
VERSION     DASTXO010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287031"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VFU96_11960"
     CDS             <1..335
                     /locus_tag="VFU96_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEU5114025.1"
                     /translation="AARDHAAPFDLVREVASTGKLPVVNFAAGGIATPADAALMMSLG
                     VDGVFVGSGIFKSSGPEAYARAIVEATTFWNEPEVVAKVSRGLGEAMPGIEIDTLRDT
                     ERLAERGW"
     gene            337..900
                     /gene="pdxT"
                     /locus_tag="VFU96_11965"
     CDS             337..900
                     /gene="pdxT"
                     /locus_tag="VFU96_11965"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007263066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEU5114026.1"
                     /translation="MTNVGVLALQGDVREHIHTLESLGVAATPVKTPNQLGSADALVI
                     PGGESTTIGKMAVRFDLLEPLRRSIEDGLPTYGTCAGLILLAGAVTDGDQPLLGVLDV
                     VVRRNAFGRQNESFESDLVVAGLDAPFHAVFIRAPWVEKVGADVQVLADIDEHPVMVR
                     QGNILATSFHPELTGDGRIHQLLLDLV"
     gene            908..1657
                     /locus_tag="VFU96_11970"
     CDS             908..1657
                     /locus_tag="VFU96_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU5114027.1"
                     /translation="MSGHSKWANIKHRKGRQDAARGKLFGKLAKAIEIAAREGGGNPD
                     FNPTLATAIYKAKVASMPNENIERAVKRGSGEVEGAHYEEIWYEGYGPGGVALYVQIL
                     TDNRNRAASDVRSAFTRGGGNLGEPGSVGYLFHQKGLIGLKGSEDDVMLVALDSGAED
                     IRDSGDGWLEVITSPGDVQTVRTALEEAGLEIDSADVTQLPSTTVRVGEGEARKLLRL
                     IDALDDLDDVQAVFSNYDIDDDVMERVLEEV"
     gene            complement(1676..1993)
                     /locus_tag="VFU96_11975"
     CDS             complement(1676..1993)
                     /locus_tag="VFU96_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU5114028.1"
                     /translation="MDWLGTIIASLLAGIIVGPLARLVLPGRQNISLGMTILIGAVGA
                     FLGGLIANWVGVEDTAGLDWTQYLIQVGVAAVLVLAYGAMAGRGSSSAPSSGSTPPPP
                     PAT"
     gene            2122..2379
                     /locus_tag="VFU96_11980"
     CDS             2122..2379
                     /locus_tag="VFU96_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007515707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU5114029.1"
                     /translation="MGILIWLLVGLIAGLIARALVPGSDSMGVLGTLVLGLVGSLIGG
                     FLGNLLFDGQWDVTTAGIVGSIIGAIIALLIYRAAASRSVA"
     gene            2497..2970
                     /gene="ruvC"
                     /locus_tag="VFU96_11985"
     CDS             2497..2970
                     /gene="ruvC"
                     /locus_tag="VFU96_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004990577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEU5114030.1"
                     /translation="MGIDPGLSATGYGVVEGGHPPRALLAGVIRTDTAAPMAERLADL
                     FIGLTQVIEESRPDVVAVESVFTNQNLQTAISVGRASGVALLAAARAGLQVSEYAPTA
                     VKSAITGDGSASKTQIQQMVARLLRLAEPPRPADAADALAIALCHLRAAPLESAR"
     gene            2967..3536
                     /gene="ruvA"
                     /locus_tag="VFU96_11990"
     CDS             2967..3536
                     /gene="ruvA"
                     /locus_tag="VFU96_11990"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU5114031.1"
                     /translation="MIGRLRGILVSRHGEVVCVEVGGVGYEVTMTPRGVSALPGVGEE
                     VVVHTHTHVREDEMSLYGFGSESDRDLFRILLGAGGVGPKLAMAILGTMTHQDIVRSI
                     VTEDADALTVVPGVGKRGAQKIVLELAPKLAGVDAEVVGSTSLVAVRQALESLGYSTE
                     EINGVMGELDPDSAIETQIKAALRALGRG"
     gene            3539..4552
                     /gene="ruvB"
                     /locus_tag="VFU96_11995"
     CDS             3539..4552
                     /gene="ruvB"
                     /locus_tag="VFU96_11995"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU5114032.1"
                     /translation="MREDSLLNPTIETDEEITLRPKHLDEFIGQDRVRERLEISLDAA
                     RKEKRPLDHVLFSGPPGLGKTTLAGVIANELEVKMRVTSGPALDRPGDLAAILSNLDT
                     GDVFFIDEIHRLPRAVEEVLYPAMEDFQLDIVLGKGPTAQSIRLDLPRFTLVGATTRK
                     GTVTAPLRDRFGIEEKLDYYSPDELAVIIRRSAGIIGVEISEEAAGIISHRSRSTPRI
                     ANRLLARVRDYAVSRASGVIHPEVANDALRVFEVDDLGLDKVDRAILESIIDKFGGGP
                     VGLSTLAIAVGEEPETVEDAYEPFLLQSGLIKRTPRGRVATARAYTHLGQEPPTDSQG
                     TLL"
     gene            4565..>5166
                     /locus_tag="VFU96_12000"
     CDS             4565..>5166
                     /locus_tag="VFU96_12000"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEU5114033.1"
                     /translation="MRVESFKYPLPESAIAQEAIEPRHDSRLLDTRDMSDHRFLDLPS
                     LLRPGDLVVVNETRVRAARLKGHRVDTGGGIELLVLEARPDGLWGALARPSRRLRPGV
                     EIEMQGIVASVVEGPKDGLIVIDMKAPDVEVAIAAAGTIPLPPYFRGTLDDPDRYQTM
                     FARSTGSAAAPTAGLHFTPTVAAGLRQRGIGIVPVDLHVG"
BASE COUNT          917 a         1672 c         1676 g          901 t
ORIGIN      
        1 ccgccgcccg cgaccacgcg gcgccattcg acctggtgag ggaggtcgct tcgacaggga
       61 agctgccagt cgtcaacttc gctgccggcg gtatcgccac acccgcggat gccgccctga
      121 tgatgtcgct cggcgtcgac ggcgtgttcg tcggatccgg catcttcaag agctccggtc
      181 ccgaggccta tgcccgggcg atcgtggagg cgaccacctt ctggaacgag cccgaggtgg
      241 tggccaaagt ctcccgcgga ctgggcgagg ccatgcctgg tatcgagatc gacaccctca
      301 gggatacgga gcgtctcgcc gagcggggat ggtgagatga ccaacgtcgg cgtcctggcc
      361 ctccaggggg acgtccggga gcacatccac accctcgaat cactcggtgt ggccgcgaca
      421 ccggtcaaga ccccaaacca actcggttcg gccgacgcac tcgtgatccc gggaggggag
      481 tcgaccacga tcggcaagat ggccgttcgc ttcgacctcc tcgaaccgct ccgacgttcg
      541 atcgaagacg gtctcccgac ctacgggacc tgtgccgggc tgatcctttt ggctggcgca
      601 gtcaccgacg gtgaccagcc gctgctcggt gtcctggacg tggtggtgcg tcggaacgcc
      661 ttcgggcgcc agaacgagtc tttcgaatcg gatctcgtgg tggccgggct ggacgcgccc
      721 ttccacgcgg tcttcattcg agccccatgg gtcgagaagg tgggtgccga cgtccaggtc
      781 ctggccgaca tcgacgagca ccctgtcatg gtccgccagg gcaacatcct cgccacctcg
      841 ttccacccgg agctcaccgg cgacgggcga atccaccaac tcctcctaga cctggtctga
      901 ggaccccatg tcaggtcatt ccaagtgggc caacatcaag caccggaagg gccgccagga
      961 cgcggcgcgg ggaaagctgt tcgggaagct cgccaaggca atcgagatcg cggcgcgtga
     1021 gggaggcggg aacccggatt tcaacccgac cctcgccacc gccatctaca aggcaaaagt
     1081 ggcctcgatg ccaaacgaaa acatcgagcg tgcggtcaag agaggcagcg gtgaggtcga
     1141 aggcgcccac tacgaggaga tctggtacga gggatacggc ccgggagggg tggccctata
     1201 cgttcagatc ctcaccgaca accgcaatag ggccgcttcc gacgtccgtt ccgcattcac
     1261 gaggggcggc ggcaacctcg gcgagccggg ctcggtcggc tatctcttcc atcagaaggg
     1321 gctcatcggg ctgaagggca gcgaggacga cgtcatgctg gtcgcgctcg actcgggggc
     1381 agaagacatc cgcgattcgg gggatgggtg gctcgaggtc atcacctccc cgggcgacgt
     1441 tcagacggtt cgcaccgccc tcgaagaggc aggcctcgag atcgattccg ccgatgtcac
     1501 gcaactcccc tccaccacgg tccgggtcgg cgaaggggag gcgcgtaaat tgctgcgtct
     1561 catcgacgcc ctggacgatc tcgacgatgt ccaggccgtc ttctcgaatt acgacatcga
     1621 cgacgacgtg atggagcgcg tcctcgaaga agtctgatcg gaagcgttca gcgggtcagg
     1681 ttgccggcgg tggcggaggt gtgctcccgc tggacggggc actggaggat cccctgccgg
     1741 ccatcgctcc gtaggccaac accaacaccg cagccacacc cacctggatc aggtattggg
     1801 tccagtcgag acctgcagtg tcctcgacac cgacccagtt ggcgatcagg ccaccgagga
     1861 aggccccgac agcaccgatg aggatcgtca tccccaacga gatgttctgt ctccccggga
     1921 ggacgagacg ggcgagaggc ccgacgatga tgcctgccag cagtgatgca atgatcgtgc
     1981 cgagccagtc catgtgcgct ccttctcttg gcgacagacg ccaaccctac gcgtcgacca
     2041 cggtcgcgtg acggcaacgt cgttttgtgt cttctgcttg cgggtaattg atcccggcca
     2101 aacccaccag gaggaacgga tatgggtatt ttgatctggt tgttggtcgg cctgattgcc
     2161 ggcctcatcg cacgagccct ggtcccgggc tcggattcga tgggagttct cgggacactc
     2221 gtgctcggtc tcgtcggctc gctgatcggg ggattcctcg ggaatctgct tttcgacggg
     2281 caatgggatg tgaccaccgc cgggatcgtg gggtcgataa tcggggcgat catcgctctg
     2341 ctgatctatc gggccgcggc ctcgcgcagc gtggcataac ccgcgagcca gcgacaagga
     2401 tgatgagggg tgccgtgacc ggcacccctc atttgtttgc ccaaatctcc gaaatcgcga
     2461 tcaaaccggc ctaacctcac caaacatatg ttcgtgatgg gcatcgaccc cggtctctct
     2521 gccaccgggt acggggtggt ggagggcggg catcctcccc gcgctttgtt ggccggggtg
     2581 atccgaaccg acactgccgc tcccatggcg gagagacttg ccgatttgtt catcgggttg
     2641 actcaggtca tcgaagaatc gagacccgac gtggtggccg tcgagtcggt gttcaccaat
     2701 cagaacctgc agacggcgat ttccgtgggt cgagcctccg gtgtcgccct gctcgcagcc
     2761 gcccgcgccg gcctccaggt ctccgagtac gccccaaccg cggtcaaatc ggccatcacc
     2821 ggcgatggat cggcctccaa gacccaaatc cagcagatgg ttgcccgtct gcttcgtctg
     2881 gccgagccgc cgcggccggc cgacgccgcc gacgccctgg cgatcgcact ctgccacctt
     2941 cgcgcggcac ccttggagag tgcccgatga tcgggcgttt gcggggaatc ctcgtctcga
     3001 gacacgggga agtcgtgtgt gtcgaggtcg gcggggtggg ctacgaggtg acgatgacac
     3061 cacgcggtgt ctccgccctg cccggcgtgg gcgaggaggt ggtggtgcac acccacaccc
     3121 atgtacgcga ggacgagatg agtctctatg gattcggctc cgagtcggat cgcgatctgt
     3181 tccgaatcct gctcggggcc gggggggtgg gacccaagtt ggcgatggcg atcctgggga
     3241 ccatgaccca ccaggacatc gttcgatcca tcgtcaccga ggacgccgac gcgctcaccg
     3301 tcgtgcctgg agtagggaag cgtggggcgc agaagatcgt gctcgagctg gcacccaagc
     3361 tggccggcgt cgacgccgaa gtggtcggtt cgaccagtct ggtggcagtc cgtcaagcct
     3421 tggagagcct tggctattcc accgaggaga tcaacggcgt gatgggtgaa ttggacccgg
     3481 attcggcgat agagacgcag atcaaggcgg ccctgcgcgc cctgggcagg ggttagggat
     3541 gagagaagac tcactgttga accccacgat cgaaaccgac gaggagatca cgctccggcc
     3601 gaagcatctc gacgagttca tcggccagga ccgcgtgagg gagcgtctgg agatctcatt
     3661 ggacgcggcg cgaaaagaga agcgaccact cgaccacgtg ctgttctcgg gccctcccgg
     3721 cctgggtaag acgaccctcg caggtgtcat cgccaacgag ctggaagtga agatgcgtgt
     3781 cacctccggc ccggcgctcg atcgccccgg cgacctggcc gccatcctct ccaatctgga
     3841 tactggcgat gtgttcttca tcgacgagat ccaccggctt ccccgcgcgg tcgaggaagt
     3901 cctctatccg gccatggagg acttccaact ggacatcgtg ctgggcaaag ggcccacggc
     3961 gcaatcgatc cgtctcgacc tcccgcgatt caccctcgtg ggcgcgacca cccggaaggg
     4021 aacggtgacg gcaccactcc gggatcgttt cgggatcgag gagaagctcg attactactc
     4081 gcccgatgag ctggccgtca tcatccgtcg ctcagccggc atcatcggtg tggagatctc
     4141 cgaggaagca gccgggatca tctcccatcg cagccggtcg acccctcgta tcgccaaccg
     4201 actgctcgca agggtccggg attacgccgt gtccagggca tcaggcgtga tccaccccga
     4261 ggtcgcgaac gacgccttgc gtgtgttcga ggtcgacgac ctcggtttgg acaaggtcga
     4321 tcgggcgatc ctggagtcga tcatcgacaa gtttggaggc ggccctgtcg gcctctccac
     4381 cctggccatc gccgtcggcg aggagccgga gacggtcgag gacgcctacg agccgttcct
     4441 gctccaatct gggctgataa aacgcacgcc ccggggccgc gtggccaccg cccgcgctta
     4501 cacacacctt ggccaggaac cacccaccga ctcgcagggg acgctgctct agcctcgtcc
     4561 ctcgatgcgg gtcgagtcct tcaagtatcc cctcccggag tcggccattg cccaggaggc
     4621 gatcgagccc cgccacgact cccgattact cgacacgcgg gacatgtccg atcatcgctt
     4681 cctggatctg ccctcattgc tccggcccgg ggacttggtg gtggtcaacg agacccgggt
     4741 ccgtgcagcc cggttgaagg gccaccgagt cgacaccggc ggcggtatcg agttgctcgt
     4801 cctcgaagcc cgtcccgacg ggttgtgggg ggcgctggcc cggccttcgc gaagactgcg
     4861 accaggggtc gagatcgaga tgcaggggat tgttgcgtcc gttgtcgagg gtccaaagga
     4921 cggattgatc gtgatcgaca tgaaggcgcc cgacgtggag gtggccatcg cggctgccgg
     4981 gacaatccct ctccctccct acttccgcgg cactctcgat gaccccgacc ggtaccagac
     5041 catgttcgcc cgcagcaccg gttcggccgc cgcgcccacg gccggtctcc atttcacgcc
     5101 tacggtggcc gccggtctcc ggcaacgggg aatcgggatc gtgccggtag acctccacgt
     5161 tggaat
//
LOCUS       DASTXO010000564         7108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1839900, whole genome shotgun sequence.
ACCESSION   DASTXO010000564 DASTXO010000000
VERSION     DASTXO010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7108
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1839900"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VFU96_12005"
     CDS             <1..294
                     /locus_tag="VFU96_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5114034.1"
                     /translation="NIDKTDTLTEFWSCTQFSRAAVTFRSCSGNINKADASAESWSCE
                     FRFLGSTCTGNIDKTDTAPETWACEVADAIDFPETCSGDRTWLIPIVYIPFSI"
     gene            complement(693..776)
                     /locus_tag="VFU96_12010"
     tRNA            complement(693..776)
                     /locus_tag="VFU96_12010"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(740..742),aa:Leu,seq:cag)
     gene            860..1510
                     /locus_tag="VFU96_12015"
     CDS             860..1510
                     /locus_tag="VFU96_12015"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FhaA domain-containing protein"
                     /protein_id="HEU5114035.1"
                     /translation="MSLARSLERRLERLLEGATGRVFSGRLHPSEMAGRIAREADLSR
                     YRHESGPATANKYVLTVSPADLDVDPTTLEKKLEMALAEHAASVGLLFEGPPTVRIIT
                     SDQMAPGQFSCTPSVEPGPLPPWARLSGSGASIEIGANRVTLGRSEEADIVLPYDNVS
                     RKHALIWRSEGKSWIQDLGSSNGTSVDGAGVGAQPVPLQNGSVLSLAGHRYRFIEA"
     gene            1513..1923
                     /locus_tag="VFU96_12020"
     CDS             1513..1923
                     /locus_tag="VFU96_12020"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEU5114036.1"
                     /translation="MPDIILALLRIIFLGLVYLFVWQVARAIGAHLGISLRRKRREGT
                     KVVMVKSETQQGTELEVEDVTVLGRSPEADVLLDDPYASEFHMRLVAQENGMVLHDLG
                     STNGTYINGRRVTAPTTLRRGDTIQVGKTVMEVR"
     gene            1920..2627
                     /locus_tag="VFU96_12025"
     CDS             1920..2627
                     /locus_tag="VFU96_12025"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEU5114037.1"
                     /translation="MRYVWGSATHQGMVRQNNEDSLFPDKSGESDGRAVLMVADGMGG
                     HIAGEVASRLAINAAASAELEPDDRVAAANRAIREEVARQPDLEGMGTTLTLVELEPE
                     GVARLAHVGDSRAYLYRDGELRQLTEDHTVAAEYVALGHISAEEANDHPQSQMLTRCL
                     GLTRFVNVDRIDLNLNPGDRLLLCSDGLNSMVPTEGIAKALTDGTADEAAWKLVEAAN
                     KAGGHDNISVIVIDVAT"
     gene            2632..3939
                     /locus_tag="VFU96_12030"
     CDS             2632..3939
                     /locus_tag="VFU96_12030"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU5114038.1"
                     /translation="MTRNTEGALIVAASVLAAFGVTLVNFAQERSVDAQTALTFLVFL
                     IAFGGLHMAVRAFAPRATPLLLAPVTALTALGFFQIYRLDFAAGTDRASFQRWWILIA
                     AGLASLTLLLFNRSSISILRRYRNITLLISLVLILLPNLPAGWGFPLRGLSVNGSRLW
                     VLLDVGFTTFQFQPAELAKLGLVAYVAGYLADHQKALREARRHIGPIAFPEPRQLIPL
                     LITWGVSVLILISQRDLGASLLLFVGFVLLLYAATGTTGYLVAGGFLGLIAGLASYLA
                     FDHVQRRVIAWVAPFEHYEDEGFQIAQGLFAMGTGSLSGAGPGLGRPDLIPNAATDFI
                     FAAVGEELGFAGTVSVLALFALTISVGLGIAFRSRDTFRKLLAAGLTFVLAVQTILII
                     GGVVRLVPLTGITLPFMSYGGSALVGNLILIALLLRISHEEAT"
     gene            3936..5393
                     /locus_tag="VFU96_12035"
     CDS             3936..5393
                     /locus_tag="VFU96_12035"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEU5114039.1"
                     /translation="MNGPVRRLSIGLFGGFLALLLAVTWIQVIHADVLKADPRNSRPA
                     LSERGKERGLIVTIDGTVVAQSVPDPEDPRSFVRQYPEGPPFAHVVGYNSFLLGSSGL
                     ENAYADELRSRRDLTISDLVSVILGRDLRPESLELTINADLQRAAYEGLGGLKGGVVA
                     IDPRTGAVLTSVSSPSYDPESLLGGDAAEVWESLLADPDGPLLDRATREIYPPGSTFK
                     TVVTAAALDTGTAVPETEFPDPEEFDLPGSSATISNAGGGVCNDGRSITLLQAFIVSC
                     NTTFADLSIQVGAEDIGITAEALGFNTEIPFPWTVPEAIYPVDLLAADPAALAQSGIG
                     ERDVRAAPLHMAMVAAAVANNGMTLTPYLVNRIFDADGNTVETFEPIPMGQAMDPATA
                     SVLTEMMQDVVSEGTGQAAAIPGISVAGKTGTSMGLDGLPNPWFIGFAPAEDPTIAIA
                     VFFEGSPELGESATGGGVAAPLAAELIELWLEGSP"
     gene            5390..>7108
                     /gene="pknB"
                     /locus_tag="VFU96_12040"
     CDS             5390..>7108
                     /gene="pknB"
                     /locus_tag="VFU96_12040"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEU5114040.1"
                     /translation="MSWDETIPTIRPASMDAQTALTDRYQITSHLARGGMADVYEGRD
                     NLLNRRVAIKVLHSQYSADEAFVKRFRREAQAAANLSHPNIVGIYDWGQAQNTYFIVM
                     ELVDGRSLRDVLKSEGALLPRRATEITAEVAAALSVAHQAGLVHRDVKPGNILLAKDG
                     TVKVTDFGIARAWDDSQELTRTGAVIGTATYFSPEQAQGAAADARSDVYSLGVVLYEM
                     LTGRPPYTGDSPMSVAFQHVSTEAPLPSSLNADVSPSLDAVVAKAMRKDPVARYQTAD
                     EMRADLLSVLKGEEVGMPPVVLPVPVPANGDGATRVMSTTPVPPATAPPDEMYREIEE
                     EPSSQLPFILTAFGLLLALGGLIFLLFQLAGGGEEEPELITVPNVVDLLQDDALRRLQ
                     EEGLIGVVQFESHETVEVGTVIRTEPPAGTEVEPDTTIQVFVSSGPEEIAVPPVVGQT
                     RAEAERLITEAGLTVGRVTERADATFAEGIVIEQDPPQGVRVGVDAPVNLVVSTGPET
                     VIVPGLVNLSERDATAQLQALGLLVRVQDEYSDSVAQDLVIRSDPVADTEVQLGDTVL
                     IVVSLGP"
BASE COUNT         1247 a         2357 c         2220 g         1284 t
ORIGIN      
        1 aacatcgaca agaccgacac gctcacagaa ttctggtcct gcacacaatt cagtcgagct
       61 gctgtcacat tcaggagctg ctcggggaac atcaacaaag cggacgcctc ggcagagtcc
      121 tggtcctgtg agtttcgttt cctcgggtcc acctgcaccg gcaacatcga caagacggac
      181 acagctccag agacatgggc atgcgaagtt gctgacgcca tcgacttccc cgaaacgtgt
      241 tcgggtgacc gaacgtggct catcccgatc gtgtacatcc cattctcgat atgaacccga
      301 gaagcaatgg gccgagaaaa ggaatcaagg ctcgctccgc ggtgactgtc ttgcgttcgc
      361 actggacgtc cgcgccgaag ttggtgtctt cggtgggatg acccggcaga gcggtccagg
      421 aggctagggc cccgatgagg ggcggatatg gcgccgtcta agccgttttt cgcgtttgtc
      481 gtgacccgta atgccgccca aacgactaac ccccccactc gccggcctct gattgctatc
      541 acgatggatc gtcacagcca tgtgatcgag gggatggacc gggcagccgc cgagaccgtg
      601 gcagcgctca tcgaacacac gtccggctga aatgattgaa atcggattgc agtcggaccc
      661 tgtcggttga ttccaacgtt cgaaaacgct agtgggcgag gggggacttg aacccccacg
      721 atcttgcgat cacaggaacc tgaatcctgc gcgtctgcca attccgccac tcgcccggga
      781 tcgaggcgaa atcgtaccgt tgcaccgggg gttccggtag tcggcaacac tgtccccgac
      841 ccaccgtaac ctctcgtcgg tgagcctggc acgaagcctg gaaagacgtc tcgagcgcct
      901 cttggaaggc gccacgggac gcgtgttctc cgggcggctc cacccttccg agatggcagg
      961 acggatcgcc cgagaggcgg atctgagccg ttaccgccac gaatcgggcc cggccaccgc
     1021 caacaaatac gttctcaccg taagcccggc ggatctcgac gtggatccca ccacactcga
     1081 gaagaaactg gagatggccc tcgctgagca cgccgccagc gtgggtctcc tgttcgaggg
     1141 ccctcccacc gtcaggatca tcacgtcgga tcagatggcg ccagggcagt tctcctgcac
     1201 accgtcggtc gagcccggcc cactgccgcc ttgggccaga ctctccggtt cgggcgccag
     1261 catcgagatc ggagccaacc gcgtgactct cggaagatcg gaggaggccg acatcgtgct
     1321 tccctacgac aacgtgtcac gaaagcatgc tctgatctgg cgctccgagg gcaaatcctg
     1381 gatccaggat ctcggctcct cgaacgggac ctcggtcgac ggagcgggcg taggggcgca
     1441 accggtgccg ctgcagaatg gatccgtgtt gagcctggca ggacaccgct atcggttcat
     1501 cgaggcgtag ggatgcccga catcatcctg gcgttgctcc gaatcatctt tctgggcctc
     1561 gtctacctgt tcgtttggca ggtggcccgc gctatcggcg cacatctagg aatatctctt
     1621 cgacggaaac gtcgcgaggg aacaaaggtc gtgatggtca agtcggaaac ccaacaaggc
     1681 acagagctcg aagtggagga tgtgacggtg ctcgggcgct cgcccgaggc cgatgtcctg
     1741 ctcgacgacc cctacgcttc tgagttccac atgcggctgg tggcccagga gaatgggatg
     1801 gtgctgcatg atctcgggag cacgaacggg acctacatca acgggcgtcg agtgaccgcg
     1861 cccacaaccc tgcgccgcgg ggacacgatc caggtgggga agacggtgat ggaagtccga
     1921 tgagatacgt gtggggctcg gccacccacc agggaatggt gcggcagaac aacgaagatt
     1981 ccctgttccc cgacaagtca ggcgagtcgg acgggcgtgc cgtgctgatg gttgccgacg
     2041 ggatgggcgg gcacattgcc ggcgaggttg cctcccggct cgccatcaac gccgccgcct
     2101 cggccgaact cgaacccgac gaccgggtcg ctgccgccaa ccgtgccatt cgcgaggagg
     2161 tcgcacgtca gcccgatctc gaagggatgg ggaccacctt gaccctggtc gagctcgagc
     2221 ccgaaggagt cgctcgactc gcccacgtcg gtgacagtcg tgcgtatctg tatcgggatg
     2281 gagagctgag acagctgacc gaggatcaca cagtggccgc cgaatacgtg gcgctgggcc
     2341 acatctccgc tgaggaggca aacgaccatc cgcagagcca gatgctcacc cgctgcctcg
     2401 gtctcaccag gttcgtgaat gtcgaccgca tcgacctcaa cctcaatccc ggtgaccggc
     2461 tgctgctctg ctccgacggc ctcaactcga tggtgccgac cgagggcatt gccaaagccc
     2521 tgaccgacgg aaccgctgac gaggcggcgt ggaagttggt ggaggcggcc aacaaggccg
     2581 gggggcacga caacatcagc gtcatcgtca tcgacgtcgc cacctgatca gatgactcgc
     2641 aacaccgagg gcgcgttgat cgtggcggcg tccgtgctgg cggccttcgg cgtcacgctg
     2701 gtcaatttcg cgcaggagcg cagtgtcgac gcccagaccg cgctgacctt cctcgtcttc
     2761 ctcattgcct tcggcgggct ccacatggca gtaagggcat tcgcacccag agccactccc
     2821 ctcctgctcg ctcccgtgac cgccctgacc gccctggggt tcttccagat ctatcggctc
     2881 gacttcgccg ccggaaccga tagggccagc tttcagaggt ggtggatcct gatcgccgcc
     2941 gggctggcgt cgctgacgct gctgttgttc aaccggagct cgatctccat cttgcgccgc
     3001 taccgcaaca tcaccttgct catctccctc gtcctgatcc tgcttcccaa cctccccgcc
     3061 ggatggggct tcccccttcg gggattgtcg gtgaacggct cccggctctg ggtcctcctc
     3121 gatgtgggtt tcacgacctt ccagttccag ccggcggagc tggccaaact cgggctggtc
     3181 gcctatgtcg ccgggtatct cgccgatcat cagaaggcgc tccgagaggc gcggcgtcac
     3241 atcgggccca tcgcctttcc cgagccccgc caactgatcc cactcctcat cacctggggg
     3301 gtcagcgtgt tgatactgat atcgcaacgt gatctcggcg cctcgctgct cctcttcgtg
     3361 gggttcgtcc tgctgctcta cgccgccacc gggaccaccg ggtacctggt ggcgggggga
     3421 ttcctcggcc tgatcgcggg tctggcgtcg tacctcgcct tcgaccacgt tcagcgacgg
     3481 gtgattgcct gggtagctcc gttcgagcac tacgaggatg aaggcttcca gatcgcccag
     3541 gggctcttcg ccatgggaac ggggagcctc tcgggggcgg gcccggggct ggggaggccc
     3601 gacctcatcc ccaacgcggc tactgacttc attttcgccg cggtgggtga ggagttggga
     3661 ttcgccggaa ccgtgtcggt tctcgcgctg ttcgcgctca ccatctcggt gggcctcggc
     3721 attgccttcc gtagccgcga taccttccgc aagcttctgg ctgccggact caccttcgtt
     3781 ctggcagtcc agaccatctt gatcatcggc ggcgtggttc ggctcgtgcc cctcaccggg
     3841 atcaccctcc cattcatgtc atacggcggc tctgccctgg tggggaacct gatcctgatc
     3901 gccctcctgc tcagaatcag tcacgaggag gcaacgtgaa cgggcccgtt cgccggcttt
     3961 ccataggcct gttcggcggc ttcctggcct tgctcctggc ggtcacctgg atccaggtga
     4021 tccacgccga cgtgctcaag gccgacccgc gcaactcccg accggctttg tccgagcgcg
     4081 gcaaggagcg tgggctgatc gtgaccatcg atgggacggt tgtggcgcaa tcggtcccgg
     4141 accccgaaga cccccgctcg ttcgtgcgac agtatcccga ggggcctccc tttgcccatg
     4201 tcgtcggcta caactcattc ctcctcggat cgtccgggtt ggagaacgcc tacgccgatg
     4261 agcttcgctc ccgaagggat ctgacgatct ccgatctcgt atcggtcatc ctgggccgag
     4321 acctgcgccc ggagagtctc gagctgacga tcaacgccga cctgcagcgg gcggcgtacg
     4381 agggacttgg cgggctgaag ggtggggtgg tcgccatcga tcccaggacc ggggcggtgc
     4441 tgacctccgt ctcctctccg tcctacgacc cggagtcgct cctgggcggc gacgcagccg
     4501 aggtatggga gagcctcctg gctgatcctg acggtccgct cctcgaccgg gcaaccaggg
     4561 agatctaccc gccgggctcg acattcaaga ccgtggtgac cgcggcagct ctcgacaccg
     4621 gcacggccgt tcccgagacc gagttccccg atcccgaaga gttcgacctg cccggatcct
     4681 cggcgacgat ctccaatgcg ggaggaggcg tctgcaacga cgggcggtcg atcaccttgc
     4741 ttcaggcatt catcgtctct tgcaacacga cctttgccga tctctccatc caggtggggg
     4801 cagaagacat cgggatcacg gccgaggcgc tgggtttcaa caccgagatc cccttcccgt
     4861 ggacggttcc cgaggcgatc tacccggtcg atctgctcgc ggcagaccct gccgcgctgg
     4921 cccagagcgg catcggggaa cgagacgtgc gcgccgcgcc gttgcatatg gcgatggtgg
     4981 ctgcggcggt cgccaacaac ggcatgaccc tcacccccta tctggtgaat cgcatcttcg
     5041 acgcagacgg aaacaccgtg gagacattcg aaccgatccc catgggtcag gccatggacc
     5101 ctgccaccgc ctcggtcctg accgagatga tgcaggatgt tgtcagcgag ggcacgggcc
     5161 aggcagcggc gatccccggc atcagcgtcg cgggcaagac cgggacctcg atggggctcg
     5221 acgggctgcc caacccgtgg ttcatcgggt tcgccccggc cgaggaccca accatcgcca
     5281 tcgcagtctt cttcgaggga agcccggagc tgggtgagtc agccaccggc ggcggtgtcg
     5341 ccgcccctct cgccgccgag ctcatcgagc tgtggctgga gggatcccca tgagttggga
     5401 tgagaccatt cctacaatcc gcccggcttc catggatgcc caaaccgcat tgaccgaccg
     5461 ttatcaaatc acgtcccacc tggcccgggg cgggatggcc gacgtctacg agggcaggga
     5521 caacctgctc aatcgcaggg tcgcgataaa ggtcctccac tcccagtact ccgccgacga
     5581 ggcattcgtg aagcggttca gacgggaggc acaagccgca gccaacctga gccaccccaa
     5641 catcgtcggc atctacgact ggggacaggc gcagaacacc tatttcatcg tcatggagct
     5701 ggtcgacggt cgttccctgc gcgacgtcct caagtccgag ggggccctgc taccccggcg
     5761 tgccaccgag atcaccgccg aagtcgccgc agccctctcg gtggcccatc aggccggcct
     5821 cgttcatcgc gacgtgaaac cgggcaacat cctcctcgcc aaggacggca ccgtgaaagt
     5881 gaccgacttc ggcatcgccc gcgcctggga cgacagccag gagctgaccc ggaccggcgc
     5941 cgtcatcggg accgccacct acttcagccc cgaacaggcc caaggggcag cagctgatgc
     6001 tcggagcgac gtgtactcac tcggcgtcgt gctctatgag atgctcaccg ggcgaccccc
     6061 ctacaccggg gatagcccga tgtccgtcgc ctttcagcat gtctccaccg aggccccact
     6121 tcccagctcg ctcaacgccg atgtgtcgcc ttcgctggac gcggtggtgg ccaaggcgat
     6181 gcgaaaggat ccggtcgccc ggtaccagac ggccgacgag atgcgcgccg atctcctctc
     6241 ggtgctcaag ggagaagagg tcgggatgcc gcctgtcgtg ctcccggtgc cggtgccggc
     6301 caacggtgac ggagcgaccc gggtgatgtc gaccaccccg gtgccaccag ccaccgcccc
     6361 acccgacgag atgtaccggg agatcgagga ggagccgtct tcacagttgc ccttcatcct
     6421 cactgcattt ggcctgctgc tcgctcttgg tgggttgatt ttcctgctct tccaattggc
     6481 cggcgggggc gaggaggagc ccgagttgat caccgttccc aacgtcgtcg acctccttca
     6541 ggacgatgcc ttgcgccggc tccaggaaga gggcctgatc ggcgtcgttc agttcgaatc
     6601 ccatgagacc gtggaagtgg ggactgtcat ccgcaccgag ccccctgcag ggactgaggt
     6661 ggaaccagac acgacgatcc aggtcttcgt ctcatccggg cccgaggaga tcgcggtgcc
     6721 acccgtcgtt ggtcagacgc gggccgaggc ggagcggctc atcacggaag cgggtctcac
     6781 cgtgggacgg gtcactgaac gcgctgatgc cactttcgcc gagggaatcg tgatcgagca
     6841 ggacccgccg caaggagtga gggtgggcgt cgatgccccg gtcaatctgg tggtgtccac
     6901 cggaccggag acggtcatcg tgcccgggct ggtcaatctc agcgagcggg atgccaccgc
     6961 ccaactccag gccctcggat tgctggtgcg ggttcaggac gagtattccg actcagttgc
     7021 ccaggacctg gtgattcgct ctgaccctgt ggcagacacc gaggtccaac tcggtgacac
     7081 ggttctcatc gtggtctcgc tgggcccg
//
LOCUS       DASTXO010000565         2223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_661094, whole genome shotgun sequence.
ACCESSION   DASTXO010000565 DASTXO010000000
VERSION     DASTXO010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661094"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFU96_12045"
     CDS             complement(<1..230)
                     /locus_tag="VFU96_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEU5114041.1"
                     /translation="MSDIDALLQEAAKRAIRYRGNLATSPITPDPAVDLGAFGGDFPD
                     GPTDPKDVLALMDDVGTPATMGFSSPRFFGWV"
     gene            complement(227..904)
                     /locus_tag="VFU96_12050"
     CDS             complement(227..904)
                     /locus_tag="VFU96_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006347979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5114042.1"
                     /translation="MSKLKVLLVDDHEVVRAGIRALLEAEDDIVVVGEAGTAAEGVRR
                     VGYDEPNVVVLDVRLPDGSGVEACRDIRSRFPDVKVLMLTSFADEEALMSAILAGASG
                     YVLKRVKMTDLVADIRRVGAGESLLDADMTERLFARLRNGPEDDPLLAKLSKQERTIV
                     GYIADGLTNKQIADEMFLAEKTVKNYVSNLLATMGLSRRSEAAAYVARIEGEHESAEA
                     PQGWEEV"
     gene            938..2047
                     /locus_tag="VFU96_12055"
     CDS             938..2047
                     /locus_tag="VFU96_12055"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEU5114043.1"
                     /translation="MSPTTAVQRLPELIEAAAAVVGAGDLEQVLRRLVAEARAATGAR
                     YAALGVIGEHGVLTEFIHEGLTREQVDRIGSLPTGKGVLGTLVRENRTIRLDSIGDHP
                     DSYGFPPNHPPMGNFLGVPVSAGGKAFGNLYLTEKEGGFTDDDVMLVEALSRIAGSAV
                     TTARLQSRLRTIAVGEDRERIARDLHDSVIQDLFAVGLALQGIADRITDETAASTIEE
                     SVDRLDRVVESLRAYIFQLRTTGTRRQLDDRLQELVSRMGSAYPAAVTLEIGTAELPA
                     EQLEDEVIKFVTEALSNALRHSGGEEVAVLVDADGDWCRISVSDNGVGIDPAPSSTGM
                     GLANLTSRAASLGGHADVRSNKGGGSTVEVRLPIR"
     gene            complement(2040..>2223)
                     /locus_tag="VFU96_12060"
     CDS             complement(2040..>2223)
                     /locus_tag="VFU96_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5114044.1"
                     /translation="IVSHIGPDHTRIIMDGTYQPPLGPFGRVVDRVLLRHVAEATVKT
                     WVDRLAESLMAGRSIT"
BASE COUNT          388 a          706 c          735 g          394 t
ORIGIN      
        1 atcacccacc caaagaagcg ggggctggag aaccccatag tcgccggcgt gccgacgtcg
       61 tccatgaggg cgaggacgtc cttcgggtcg gtcggcccgt ccggaaagtc gcccccgaat
      121 gcgccgagat caactgcggg atcaggcgtg atcggactgg tggccagatt cccgcggtac
      181 ctgatggcgc gttttgccgc ttcctggagg agcgcatcga tatcgctcaa acctcttccc
      241 agccctgcgg cgcctcggcg ctctcatgct cgccctcgat ccgggcgacg taggccgccg
      301 cctcgctccg ccggctcagt cccatcgtgg ccaggaggtt ggacacatag ttcttgacgg
      361 tcttctcggc caggaacatc tcgtcggcga tctgcttgtt ggtcagccca tcggcgatgt
      421 agccgacgat cgtcctctcc tgtttgctca gcttcgccag caggggatcg tcttctggcc
      481 cgtttcggag gcgggcaaag agacgctcgg tcatgtcggc atcgagcagg gattcccctg
      541 cgccaacacg acgaatgtcg gcgacgaggt cggtcatctt gacccgcttg aggacatagc
      601 cggacgcccc ggcgaggatc gcggacatca gcgcttcctc atcggcgaag gaggtcagca
      661 tcaggacctt gacgtcggga aaacgcgagc ggatgtcacg gcaagcctcg acgccacttc
      721 cgtcggggag tcggacatcc agcacaacga cgttgggctc gtcgtagcca actcgacgca
      781 cgccctcggc cgcggttcct gcctccccga cgaccacgat gtcgtcctcg gcttcgagca
      841 gcgctctgat acctgctcga accacctcgt ggtcgtccac taggagcact ttcagcttcg
      901 acatcgagaa aaacggtagc ggtatggtct ggccccgatg tcgccgacca cagctgtgca
      961 acgtctgccc gagttgatcg aagccgccgc tgcggtcgtc ggggcaggcg acctggagca
     1021 ggtccttcgt aggctcgttg ccgaggccag ggccgccaca ggagcgcgat acgctgcgct
     1081 cggggtcatt ggcgagcacg gcgtgctgac cgagttcatt catgaggggc tcacgcggga
     1141 gcaggtcgat cgcatcgggt cgctccccac cgggaaaggg gtgctcggga ccctggtgcg
     1201 ggagaaccgg acgatccgtc tcgactcgat cggggaccat cctgactcgt atggattccc
     1261 cccgaaccac ccgcctatgg gcaatttcct gggtgttccc gtctcggccg gagggaaggc
     1321 attcggcaat ctctacctga ccgagaagga aggagggttc accgacgacg acgtgatgct
     1381 ggtcgaggcg ctcagtcgca tcgccggctc ggcggtgacc acggcgcgtc tccagagtcg
     1441 cctccgcacg atcgcagtgg gggaggatcg ggagaggatt gcccgggacc tccacgactc
     1501 tgtgatccag gacttgttcg cagtgggctt ggctctgcag gggatcgccg accggattac
     1561 tgatgaaacc gcagcctcca cgatcgagga gtccgtcgac cgcctggacc gcgtggtcga
     1621 gtccctgcgc gcctacatct tccaactgag gacgacggga acgcggcgtc agctcgatga
     1681 tcgccttcag gagctcgttt cccggatggg ctcggcatat ccggcagccg tcaccctcga
     1741 gataggcacg gccgagctgc ccgccgagca actcgaggac gaggtgatca agttcgtcac
     1801 ggaggcactg agcaacgccc tacgccactc tggcggcgag gaggtcgcgg tgctcgtcga
     1861 cgctgacggg gactggtgcc gaatttcggt gagtgacaat ggcgtgggga tcgatccggc
     1921 cccttcgtca accgggatgg gtctggccaa cctcacctcc cgagcagcga gtttgggtgg
     1981 tcacgccgat gtgaggagca acaagggcgg cgggtcgacg gtggaggtgc ggctcccgat
     2041 caggtgatgg agcgcccggc catgagtgac tcggcgagac gatccaccca ggtcttgacg
     2101 gtcgcctcgg ccacgtggcg cagcaacacc ctgtcgacga cccgtccgaa aggccccaac
     2161 gggggctgat acgtgccgtc catgatgatc ctggtgtggt ctggaccaat gtgagagacg
     2221 atg
//
LOCUS       DASTXO010000566         2904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1250590, whole genome shotgun sequence.
ACCESSION   DASTXO010000566 DASTXO010000000
VERSION     DASTXO010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250590"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1148
                     /locus_tag="VFU96_12065"
     CDS             <1..1148
                     /locus_tag="VFU96_12065"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU5114045.1"
                     /translation="LEHLAGLPGVQCLERPRGPEALGAVLEGAIGRVEPGVRGPAPLT
                     TAGKTVVSDIVGQSPQIREVFAKIEKVAAGSANVCIVGESGTGKELIARAIHYNSDRR
                     DRPLITLDCTAIPEGLMESHLFGHVRGSFTGAVENRDGVFSLAHTGTLFIDEISELSL
                     PLQAKLLRVIQMREFVKVGGSKPIRTDIRLITASNKDLRRAVADGSFREDLYYRIAVV
                     MIQTPALRERKGDIVLLVDHFLKKFSGAHRKRIRSVTPAAMELLTSYQWPGNIRQLEN
                     CIEQAVVLCEGDVVDVGALWLGDTAAARPTDSSIRIRSGLTLRDVEQQYILRTLQETG
                     GNRTRAARVLGISLRCLQYKLKAYAEAGVEAPAQPNRASEPEHLLGV"
     gene            1152..1991
                     /locus_tag="VFU96_12070"
     CDS             1152..1991
                     /locus_tag="VFU96_12070"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEU5114046.1"
                     /translation="MHSRSRRLALSLIAALVLLAVPAALTVAQQPQQDASYTIGPEDV
                     LEVQVWDNKDLNQVVFVRPDGKTSLPLIGEIQAGGRTVQQLQDELVKHYSKTIKVPSV
                     TVIIREIKSRPVYFIGGFAKPGPLQLTRNDMTLLEATAMIGGVAANADAEKGFVLRAG
                     KKIPLNFDKLQKGDVSQNLKLEPFDSIVVPVAELVYVQGEVRAPGPVKFTSDLTLAKA
                     ITQVGGTTPLAAPGRVELLRSEGEKKVRMRVDLDKILRSPEDNPDVKLRPEDIIFVPQ
                     RLF"
     gene            2188..>2904
                     /locus_tag="VFU96_12075"
     CDS             2188..>2904
                     /locus_tag="VFU96_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5114047.1"
                     /translation="MLGPDGKPVLGPDGKPFVPATPPPPSQTRIPGAFDTSIIPTGAP
                     RFTFTPSLTLAEQWTDNFFLVEKGRTDNFRTSLTLGLALLMNLANTQGSLSTGLSGVY
                     DTAPDDQNFNFFPTFTANVIHTFTPRLSLTISDTFRRDDDPFLADPNGLRTTRDTFIQ
                     NTFAISLNWLVDIFQTQFYYRNVLFMPDNGSNTISNILGGNVSMPLGALNTLTGGYEF
                     TFREGETTNDEVGQRIYASFN"
BASE COUNT          512 a         1052 c          893 g          447 t
ORIGIN      
        1 ggctcgagca tctggcgggc cttcccggag tccagtgcct tgagcgcccc cgcggtcccg
       61 aggcgctcgg cgccgtgctg gagggggcga tcgggcgcgt ggagcccggc gtacgcggac
      121 cggcgcccct gaccaccgcc gggaagaccg tggtctccga catcgtgggt cagtcgccgc
      181 agatccggga ggtcttcgcc aagatcgaga aggtggcggc cggcagcgcc aacgtgtgca
      241 tcgtcggcga gagcggcacc ggcaaggagc tcattgcccg tgccatccac tacaacagcg
      301 accggcgcga ccggcccctc atcacgctgg actgcaccgc catccccgag gggctgatgg
      361 agagccacct cttcgggcat gtccggggct ccttcaccgg ggcggtggag aaccgggacg
      421 gcgtgttctc gctcgcccac accggcaccc tcttcatcga cgagatcagc gagctgtcgc
      481 tgccgctcca ggccaagctc ctgcgcgtca tccagatgcg cgagttcgtg aaggtcggag
      541 gcagcaagcc catccgcacc gacatccgcc tcatcaccgc cagcaacaag gacctccggc
      601 gggcggtggc ggacggaagc ttccgcgagg acctctacta ccggatcgcg gtggtgatga
      661 tccagacgcc ggccctgcgc gagcgcaagg gcgatatcgt cctcctcgtc gaccacttcc
      721 tgaagaagtt cagcggcgcc catcgcaagc gcatccgctc ggtgacgccc gcggccatgg
      781 agctcctcac cagctaccag tggccgggca atatccggca gctcgagaac tgcatcgagc
      841 aggcggtggt gctctgcgag ggcgacgtgg tggacgtcgg cgccctctgg ctgggcgaca
      901 ccgcggcggc ccgtccgacg gactccagca tccgcatccg ctccggcctc accctgcggg
      961 acgtcgagca gcagtacatc ctgcggaccc tgcaggagac gggcggcaat cggacgcgcg
     1021 cggcccgggt cctcggcatc agcctgcgct gcctccagta caagctcaag gcgtacgcgg
     1081 aagccggcgt cgaggctccc gcccaaccca atcgggccag cgagcccgag catctgctag
     1141 gagtctgaaa catgcacagc cgcagccgcc gtctggccct gagcctgatc gccgcgctgg
     1201 ttctcctcgc cgttcccgcc gccctgacgg tggcgcagca gccgcagcag gacgcgtcct
     1261 acaccatcgg ccccgaggac gtgctcgagg tgcaggtctg ggacaacaag gacctgaatc
     1321 aggtggtgtt cgtgcgtccg gacgggaaga cctccctgcc cctcatcggt gagatccagg
     1381 ccggcgggcg caccgtgcag cagctccagg acgagctggt gaagcactac agcaagacga
     1441 tcaaggtgcc ctcggtcacc gtcatcatcc gggagatcaa gagccgcccg gtgtacttca
     1501 tcggcggctt cgccaagccg ggcccgctcc agctgactcg caacgacatg accctcctcg
     1561 aggccaccgc gatgatcggc ggcgtcgccg cgaatgccga cgccgagaag gggttcgttc
     1621 tccgcgcggg caagaagatc cccctgaact tcgacaagct ccagaaggga gacgtctcgc
     1681 agaacctcaa gctcgagccc ttcgactcga tcgtggtgcc ggtggccgag ctggtctacg
     1741 tgcagggcga ggtgcgggcc cccggaccgg tcaagttcac cagcgatctc accttggcca
     1801 aggccatcac ccaggtgggc ggcacgaccc cccttgccgc gcccggtcgc gtcgaacttt
     1861 tgcggtcgga aggcgaaaaa aaggtgcgta tgcgggtcga ccttgataag atcctccggt
     1921 caccagagga caatccggac gtgaagttga gaccggaaga cataatcttt gtcccccaga
     1981 ggctcttcta ggaccgaggc gacgtcgcag ggggagtcgc gagccaggcg actccccctg
     2041 atccctctcg ctcttcttct cgtcctcgcg ctggtcgtcc ccacgccggg ggtcgcgcag
     2101 cagacctcgc cgactcagac gccctcacag gctccgtcgt cgacgacccc gacgcagacc
     2161 ttcacgacgc cgggtacgca gccccaggtg ctgggccccg acggcaagcc ggtgctcggg
     2221 cccgacggca agcccttcgt ccccgcgacg ccgccccctc cgtcgcagac gcgtatcccc
     2281 ggcgccttcg acacctcgat cattccgacg ggcgcgccgc gattcacctt cacgccgagc
     2341 ctcaccctgg ccgagcagtg gacggacaac ttcttcctgg tggagaaggg gcgaaccgac
     2401 aacttccgga cgtcgctgac cctcgggcta gccttgctca tgaacctggc caatactcag
     2461 ggctccctgt cgaccggcct gtccggcgtc tacgacaccg ccccggacga ccagaacttc
     2521 aacttcttcc ccacgttcac cgccaacgtc atccacacgt tcacaccgcg tttgagcctc
     2581 accatctcgg acacgttccg gcgtgacgac gaccccttcc tcgccgaccc caacggcctg
     2641 cggaccacgc gggacacgtt catccagaac acgttcgcca tctcgctcaa ctggctcgtc
     2701 gacatcttcc agacgcagtt ctactaccgg aatgtgctgt tcatgcccga caacggatcc
     2761 aacacgatca gcaatatcct cggcggcaac gtgtcgatgc ccttgggcgc gctcaacacc
     2821 ctcaccggcg gctacgagtt cacgttccgg gaaggcgaga ccacgaacga cgaggtcggg
     2881 cagcgcatct acgcgtcgtt caat
//
LOCUS       DASTXO010000567         4514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1472164, whole genome shotgun sequence.
ACCESSION   DASTXO010000567 DASTXO010000000
VERSION     DASTXO010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472164"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1058)
                     /locus_tag="VFU96_12080"
                     /pseudo
     CDS             complement(1..1058)
                     /locus_tag="VFU96_12080"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
     gene            complement(1055..1786)
                     /locus_tag="VFU96_12085"
     CDS             complement(1055..1786)
                     /locus_tag="VFU96_12085"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020495678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEU5114048.1"
                     /translation="MADPIRVQKAISGAGLMSRRAAEEAIRQGRVSVDGMEAVLGDRV
                     DVGTQQVTLDGALLPVNPDLETYLLYKPAGVISSASDPQGRQTVVDLIDAGRRLYPVG
                     RLDYESEGLILLTNDGELANRVTHPRYGIVKRYVALVAGNPGNAEIRRLTEGVELEDG
                     PARALSAQVVGRQGGQTMVDLTMGEGRNREVRRMFEALGYVVLSLVRTAIGPIADRSL
                     KPGQSRRLSVEEIRDLLASGSGDGP"
     gene            complement(1790..2350)
                     /gene="scpB"
                     /locus_tag="VFU96_12090"
     CDS             complement(1790..2350)
                     /gene="scpB"
                     /locus_tag="VFU96_12090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEU5114049.1"
                     /translation="MREVAAVEAVLFVTEAPVPIGELAEVLELPKTRVEELLDELGRD
                     LEERGSGLILRNVAGGWRLYSHPDAYPFLERFSTSATARKLSPAALETLSVVAYRQPV
                     ARSQINEIRGVDSDSAMRTLERLGLVEESERLDIPGNPAIYVTTDLFLEKMGLESLAG
                     LPPLADHVPPADTVESLEETLRPGTT"
     gene            complement(2347..3108)
                     /locus_tag="VFU96_12095"
     CDS             complement(2347..3108)
                     /locus_tag="VFU96_12095"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEU5114050.1"
                     /translation="MTFHVKTTVYEGPLDLLLQLITKHQVEVTAVGLTEVVGEYLAFI
                     DEMKDLDLEVTSEFLLIAATLIQLKAQSLLPTRGAFDLDDELLLIEERDRLLSRLLVC
                     VTFKDVAAVLQRRLQESNRFVPRTSGVDQPIAPRPVDLVVPIDIDGLAGLAFKVFSRE
                     GAEPDLDHLDLDLPSVEIAIQEIRARVTDETMSTFEELTAQCTRSVEVAAYFLALLEL
                     ARWGLIEVSQDDWLAEIVVRGRDGGALDLDSEWAS"
     gene            complement(3122..3628)
                     /locus_tag="VFU96_12100"
     CDS             complement(3122..3628)
                     /locus_tag="VFU96_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5114051.1"
                     /translation="MPGSLGHLTRRFFDVLFATPLDSGERAAIEGWLDSGQAWAFFSQ
                     SDPDQRHGYHAALVTIAAGADLEVIRAALLHDVGKRHARLGVLARSVASVAIRLGLPL
                     TTRWRLYRDHGEVAATELAGLGCHGLIVDFARDHHGERPSGIPTSTWDLLQLADQPPK
                     TGAARVAE"
     gene            complement(3633..3989)
                     /locus_tag="VFU96_12105"
     CDS             complement(3633..3989)
                     /locus_tag="VFU96_12105"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEU5114052.1"
                     /translation="MTTLDIAGAEKALHKERAHLVHQLDELGAAETGDLRVDIDFGGS
                     FADAAAVTAERTEVLGVVESLKAQLANVDQALSKIAAGTYGICEDCGKAIPTARLEAR
                     PESVRCVDCKSKLASH"
     gene            complement(3986..>4514)
                     /locus_tag="VFU96_12110"
     CDS             complement(3986..>4514)
                     /locus_tag="VFU96_12110"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU5114053.1"
                     /translation="PDLTAVKGRRDSALLEFLYATGSRVSEAVGVDVGHVDLEDKVVI
                     LTGKGAKQRLVPIGGKAVTAVSRWLPDRLSLVTRSQRGDPLFLNMRGRRLSRQGVFDV
                     VRANAARAGIPREKVSPHVLRHSAATHMVEAGADLRTVQEILGHATISTTQIYTKVSS
                     AHLVEIYVQAHPRSR"
BASE COUNT          805 a         1520 c         1421 g          768 t
ORIGIN      
        1 cgggcgatct cgatcgccaa tgccgccagc cggtccgact ccttgtggcg gagcgatccg
       61 agcccgcgga gcttgctcgg gctttcggcg aacagggcgg ccaccgccac tgcaagcgag
      121 ccatcaggcg acccggagag atcgacgtcg acgggccgca gccgcccgtc gaggggccca
      181 acaatcgtgg tgtcctggcc cgatagcccc acgtcgcagc ccattcgctc gaggacgaga
      241 gcgatctgga gatccggttg catggattgc gatccgaggc cctcgatggt gacgcggccc
      301 ccggttattg cggcagccac catcgggtac acagccgccg acgcgtcggg ctcgatgacg
      361 atgtccctgc cgctgtaccc gtgaggctcg acccggtagc cactccggac ccgatccact
      421 gtggccccaa actccttcat catcccgacc gtcatgtcga gctagcgctg agatccttcg
      481 atccgctcgg gagtcacggt cagtccgccc tgtgccaacg gagcagtgag gagcaatgcg
      541 ctggcgaact gactggtgtg acgggaatcg accgaaaccg aacccccagg cagagtcccc
      601 gctccctcga tgaccaccgg aagatggccg tcgacagact tcgcgtcgat gccgagggcg
      661 tcgagcgctt ccacgaggcc gcccatgggt cgctcgggca gccggtctct tccgacgacg
      721 gtggttctgc catgcacgag ggaggccatg gcgatgaccg accgcgcagt caaaccggag
      781 gcaccgacgt ccaccggtat ggccggtgga cgcagccggc cgccggaacc gctcactcgc
      841 cacgaagcgc cccgatcggc gacctccacg ccgaagagct gcatcgcctc actggccgcc
      901 agtgtgtcat ccgcggccaa cggagcccgt aacctggagg gcccctcggc cagcgccgag
      961 atcaccagtg ccctgatcgt ttgggatttc gagccgggtg ggcgcaccga agagacaatg
     1021 ggtcccgcgg ccggctccac cgccaggaaa tcggtcacgg tccatcccca cttcccgagg
     1081 cgaggaggtc gcgtatctcc tcgaccgata gccgccggga ctgccctggc ttcagcgacc
     1141 ggtcggcgat cggacctatc gccgtcctga ccagagaaag caccacgtac cccaacgcct
     1201 cgaacattcg ccgcacctcg cggtttcgcc cctcccccat ggtcaaatcg accatcgttt
     1261 ggccaccctg gcgccccacg acctgtgcgg acagggcccg agccgggccg tcctccagct
     1321 cgaccccttc ggtcaatcgc cgaatctcgg cgttacccgg gttcccggct accagggcga
     1381 catatctctt gacgatcccg taacggggat gggtgacgcg gttggccagc tctccgtcgt
     1441 tggtgaggag gatcagcccc tccgactcgt agtccagacg cccgaccggg tagagcctgc
     1501 gcccggcgtc gatgaggtcg accaccgttt gccgcccctg agggtcggag gcagatgaga
     1561 tgaccccggc cggcttgtag aggagatagg tctccagatc ggggttgacc ggaagcagag
     1621 ccccgtcgag ggtcacctgc tgtgtgccca cgtccacccg atcacccagc accgcttcca
     1681 tgccgtcgac ggacaccctc ccttggcgga tcgcttcctc ggccgcccgc cgtgacatca
     1741 gcccggctcc ggagatcgcc ttctggacgc ggatcggatc cgccatcgct caggtggtcc
     1801 cggggcgcag agtctcctcg agagattcga cggtgtcggc cggaggaaca tgatcggcca
     1861 ggggcggcaa gccggctagc gactcgagac ccatcttctc caggaagaga tcggtggtga
     1921 cgtagatggc cgggttgccc gggatgtcga gacgctcgct ctcttctacc aggccgagtc
     1981 gttccagggt cctcatcgct gaatccgagt cgacccctct gatctcattg atctggctcc
     2041 gagcaacggg ctgccgatag gcgacgacgg agagggtctc cagggcggcg ggggacagtt
     2101 tgcgagctgt ggccgaagta gagaaacgct ccaggaacgg gtaggcgtcc gggtgggagt
     2161 acaacctcca acccccggcc acgttgcgaa ggatgaggcc gctcccccgc tcctccaggt
     2221 cacggcccaa ctcgtcgagc agctcctcga ctctcgtctt gggcagctcc aagacttctg
     2281 ccaattcgcc gatcggcaca ggtgcctctg tcacgaagag gacggcttcc actgccgcga
     2341 cttctctcac gacgcccact ccgagtcgag atcgagagct ccaccatctc taccccggac
     2401 gacgatctcg gccaaccagt cgtcctggga gacctcgatg agaccccagc gggccaattc
     2461 gagcaacgcg aggaaatagg cagccacctc gaccgaccgg gtgcattgcg ccgtcagctc
     2521 ctcgaaggtg gacatcgtct cgtcggtgac gcgagccctg atctcctgga tggcgatctc
     2581 gacggagggg agatccaggt ccagatggtc gagatcgggc tcggccccct cccgggagaa
     2641 gactttgaac gcaagaccgg caagaccgtc gatgtcgatc ggcacgacca ggtccacggg
     2701 tcgtggtgcg atgggctggt cgaccccgct cgttcgtggg acaaaccggt ttgactcctg
     2761 gagtcgccgt tgaagcacgg cggccacatc tttgaacgtc acgcacacca gcagacgcga
     2821 gagaagccgg tcgcgctcct cgatgagcaa gagctcgtcg tcgaggtcaa aggcgccacg
     2881 ggtcggaagc agggattggg ccttgagctg aatgagggtc gcggcgatca gcaagaactc
     2941 cgatgtcacc tccagatcca ggtccttcat ctcgtcgatg aatgccaggt actccccaac
     3001 cacctcggtg aggcccaccg cggtcacctc gacctgatgt ttcgtgatca gttggagcag
     3061 gaggtcgagc gggccctcat aaaccgtggt cttcacatgg aaggtcatca gccgcctcag
     3121 gttattccgc gactcgtgcg gcgccggtct ttggcggctg gtcggccaat tggagcagat
     3181 cccacgtcga ggtggggatg ccagagggcc tctcgccgtg gtgatctcgg gcgaagtcga
     3241 cgatcaaacc gtggcagccc aacccggcta gttctgtggc cgcgacctcg ccatggtcgc
     3301 gataaagccg ccaccgagtg gtcaatggca gccccagtcg tatcgccacc gaggcgacac
     3361 tccgagcgag cacgccgagg cgggcatgtc gcttgcccac gtcgtggagc agggcggccc
     3421 gaatgacctc gaggtcggct cccgctgcaa tggtcacgag agcggcgtgg tacccatggc
     3481 gctggtcggg atccgattgg gagaagaatg cccaggcctg cccgctgtcc agccagccct
     3541 cgatcgcggc ccgctcaccc gagtccaacg gagtggcgaa gaggacgtcg aaaaagcgac
     3601 gggtgagatg tcccaacgag cccggcatgc gtttagtggc tggccagctt gctcttgcag
     3661 tcgacacaac gcacagactc aggtcgggcc tcgagccgag ctgtcgggat tgccttgccg
     3721 cagtcctcgc agatcccgta tgtgcccgct gcgatcttgc tcaacgcctg gtcgacgttg
     3781 gccagttgcg ccttcagcga ctcgaccaca ccgaggacct cggtccgctc cgctgttacc
     3841 gccgcggcat cggcgaagct tccgccgaag tcgatgtcga cccgaaggtc ccccgtttcg
     3901 gccgcgccta gctcgtccaa ctgatggacg agatgagcac gctccttgtg gagagccttc
     3961 tccgcaccgg cgatgtccaa cgttgtcatc tgctcctcgg gtgggcctgg acatagatct
     4021 cgacgagatg ggcagaactc accttcgtgt agatctgggt ggtcgaaatg gtagcgtgac
     4081 ccaggatctc ttgtacggtc ctgaggtccg cgccagcctc caccatgtgc gtcgccgccg
     4141 agtgtctcag cacgtggggt gagactttct ccctgggaat gccggctctt gctgcgttgg
     4201 cacggacgac atcgaagacg ccctgacggc tgagacgccg gcccctcatg ttgaggaaca
     4261 agggatctcc cctttgtgat ctcgtgacga gactcaaacg gtctggcagc catcgggaga
     4321 ctgcggtcac ggccttgcca ccgatcggca ccaacctctg cttggcccct ttgccggtca
     4381 agatgaccac cttgtcctcg aggtcgacgt gtccgacgtc gacccccacc gcctccgaga
     4441 cccgggagcc ggtggcgtag agaaactcga gcaacgcgga gtcacgacgc cctttgacgg
     4501 cggttaggtc cggc
//
LOCUS       DASTXO010000568         2991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_992654, whole genome shotgun sequence.
ACCESSION   DASTXO010000568 DASTXO010000000
VERSION     DASTXO010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992654"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(299..>2991)
                     /locus_tag="VFU96_12115"
     CDS             complement(299..>2991)
                     /locus_tag="VFU96_12115"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5114054.1"
                     /translation="LVSEDERESFTNALVEAIEHRSSRVRVVITLRADFYDHPLASRQ
                     LGELLRDHTELVTPMSAVELEHAITQPAESAGVVVEPALLAALTAEATSEPAVLPMLQ
                     YTLTELFERRSGKTMTLESFDSIGGLTGAVVGRAEALFAALSSEGRSVARHVFLRLVS
                     INDVGSDTRRRALLSELQGLGGRDGDVDELLRAFARHRLLSFDRDPVSRAPTVEVAHE
                     SLIGAWSRLGSWIDRAREDVRAQRRLATAATEWIGQDRNPDFLLTGASLARYATWVSD
                     SPVRLTADERAFLEAAFAQDRLREIEAHEGSIRESRLRRRTRALVGLGALSLLVVLLA
                     VVAFTQRERAEGLATELASRDRARALIAESGLVVKDDPALGMLLAIEAMRVTESGGEV
                     LPEAVDAVHWALQEATVLYPADDADIPVAVRPHATGPRGVFALSPAELADHGRAALGR
                     PFTSTECERFFSSLPCPDTSQPIPADVTIAGGLEGYTLVGEGLPLEGTLVELTSGWSE
                     EMVEAASDDLAALGADMGVEVAFRWDGGLQTPGDIIAPGLPRMIFEMASERPVVDIGS
                     YIGEDYLRESYGEHLMSLVSRDGSSYGIPMALDPKSLIFYNPEAFAEAGYTKPASWDE
                     LITLSDRMVSDGRTPWCLGVFSFDDTGWLATDWLETIVLQSEGPEFYDQWASHQVPFD
                     HPAVVGALEKIGQLAHTTGYHPPGSVGETSWDTAMKMVSEDPPRCLMAPVSDFGSGFF
                     GEAPRTVMLFPAVNPEYTQAMEGGVGIVYTISDRPEVRAVMRGFASASWGTSNARNPN
                     GWFYPSHVGFDVAEPTDPIERIKVSALTAANQAGLFRFDASDNMPGEIGFGVLFDVLT
                     EYVSDPELSAKEALAQVEAAWKELEETEALETFETDETSS"
BASE COUNT          559 a         1004 c          907 g          521 t
ORIGIN      
        1 ctccagtttt gccagacgat cggagggcgg ccgccgcagg gccaaggcag acggcaccgc
       61 gtcccggtca cacaaactgt cccacctcga acctgacgag cagggtggcc ttgcgaccga
      121 cggctctggt tcccggtcta cgggccgctt gaggcggatc tgaccatttg atagccgcga
      181 cgctggatgg acagccgccg tcagcgttgg cagaaaccag cgcgagtgac tcttgcccaa
      241 aggtcaccct ctctattagg gcaacattgg gattcggtcg acgcaggccc aatcgggcct
      301 aagagctggt ttcatctgtc tcgaacgtct ccagtgcctc ggtctcctcg agttctttcc
      361 aggcggcctc gacctgagcc aatgcctcct tggcagagag ctccgggtcg gagacgtatt
      421 cggtcagcac atcgaacagc acaccaaaac cgatctcgcc tggcatattg tccgaggcgt
      481 cgaaccggaa cagcccggcc tgattggccg cagtcaacgc cgaaactttg atccgctcaa
      541 tagggtctgt cggttcagcg acgtcgaagc cgacatgact cggatagaac cacccgttcg
      601 gattccttgc attcgaggtt ccccaggatg cgctggcaaa cccgcgcatg accgccctca
      661 cctccgggcg gtccgagatc gtatacacga tcccgactcc cccctccatc gcctgggtgt
      721 attcagggtt gaccgccggg aacaacatca cggtccgcgg tgcttcaccg aagaaacccg
      781 atccgaaatc tgagaccgga gccatcaaac accttggtgg gtcctccgaa accattttca
      841 tggccgtgtc ccaactcgtc tccccgaccg accccggtgg gtgataaccc gtcgtgtgcg
      901 cgagctggcc gatcttctcg agggccccga ccactgcagg gtgatcgaaa ggaacctgat
      961 gagaggccca ctggtcgtag aactcggggc cctcgctttg gagcacgatg gtctccagcc
     1021 agtccgtggc cagccacccg gtgtcgtcga aggagaacac tccgaggcac catggagttc
     1081 gaccatccga caccatccga tcgctgaggg tgatcagctc gtcccatgac gcaggctttg
     1141 tgtatccggc ctccgcgaag gcctccgggt tgtagaagat caatgactta gggtccagag
     1201 ccatagggat tccgtagctt gacccgtccc gtgacacgag cgacatcaga tgctcaccgt
     1261 acgattcgcg gaggtagtcc tccccgatgt agctcccgat gtcaaccacc ggacgctcgg
     1321 aggccatctc gaagatcatc cggggaaggc ctggggcgat gatgtcgccg ggggtctgta
     1381 gccccccgtc ccaccgaaag gccacctcga cccccatgtc ggcccccagg gcagccagat
     1441 cgtccgaggc ggcctcgacc atttcttcac tccagccgct ggtcagctcg accaacgtcc
     1501 cttccagggg aaggccctct ccaaccagcg tgtagccctc caatccaccg gcgatggtca
     1561 cgtcggcggg gatgggttgg ctggtgtcgg ggcagggaag actggagaag aatctctcac
     1621 attcggtgga ggtgaacggc ctgcccaggg cagcgcgacc atggtcggcc aactccgccg
     1681 gggacaatgc gaacacccct cggggtccag tcgcatgagg gcgaaccgcc accgggatat
     1741 cggcgtcatc ggctggatac aggaccgttg cctcctgcaa tgcccagtgc acggcatcca
     1801 ctgcttccgg caagacctcg ccacccgact cggtgacccg catcgcctct atggccagca
     1861 gcattcctag cgccgggtcg tccttgacca ccaagcccga ctcggcgatc agcgctctgg
     1921 ctcggtcccg cgaagccagt tccgtcgcca gcccctcggc tcgctctcgt tgggtgaaag
     1981 cgaccactgc caggagcacc acgagcagtg agagcgcgcc cagtcccacc agggccctgg
     2041 tccggcgacg cagccgggac tcccggatcg agccttcgtg ggcctcgatc tctcgaaggc
     2101 gatcctgagc gaaggcggcc tcgagaaagg cccgctcatc tgctgtcagc ctgaccggtg
     2161 agtccgagac ccaagtggcg tagcgagcca ggctggcacc ggtgagcagg aagtcggggt
     2221 tccgatcctg gccgatccac tcagtggcag ccgtcgccag ccggcgttga gcgcgcacgt
     2281 cctcgcgggc gcggtcgatc caggagccaa gccgggacca ggcaccgatc agggactcgt
     2341 gggccacctc gaccgtcggg gcccggctca ccgggtcccg gtcgaagctg agcaatcgat
     2401 gccgggcaaa cgcgcgcagc aactcgtcga cgtctccgtc acgcccaccc agtccctgca
     2461 attcggaaag cagggctcgt cgtcgggtgt cgcttccgac gtcattgatc gagaccaggc
     2521 gcagaaagac gtgtcgagcc accgaacgcc cttccgacga gagcgccgcg aacaaagcct
     2581 cggcccgtcc caccacggcg ccggttaggc caccgatgga gtcgaagctc tcgagtgtca
     2641 tggttttgcc gctgcgccgt tcaaacagct cggtcagggt gtattggagc attggcagga
     2701 cagccggttc cgacgttgcc tccgcggtca gtgccgccaa tagcgccggt tcgaccacga
     2761 ctcctgcact ctccgcgggt tgggtgatgg cgtgctccaa ctccaccgcg ctcatcggcg
     2821 tcacaagttc ggtgtgatcc cggagcaatt cccctagttg cctggaagcg agagggtggt
     2881 cgtagaagtc ggcccgcagc gtgatcacca cgcggacccg gctcgatcga tgttcgatgg
     2941 cttcaacaag ggcgttggtg aatgactcgc gttcgtcttc ggacacaagc g
//
LOCUS       DASTXO010000569         3674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_403551, whole genome shotgun sequence.
ACCESSION   DASTXO010000569 DASTXO010000000
VERSION     DASTXO010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403551"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VFU96_12120"
     CDS             complement(<1..290)
                     /locus_tag="VFU96_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5114055.1"
                     /translation="MSERTPDEEKQPSRLWDDGDDDAEVFALPKRLEDELAEGDAAPD
                     DSDADDSAEGADDESSSKPGPGDADYEGMSFPQDLDDFEANQFTGSTTQEYQG"
     gene            complement(292..843)
                     /gene="frr"
                     /locus_tag="VFU96_12125"
     CDS             complement(292..843)
                     /gene="frr"
                     /locus_tag="VFU96_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009378354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU5114056.1"
                     /translation="MISDLLTEAEHKMDQAIDHVASEFSTVRTGRANPQLLQRITVEY
                     YGSPTPLQQLASISVPEPRLLVVQPFDKSTVGDIERALQTSELSLNPTNDGNVIRIAF
                     PPLTEERRKDLIKVVRNMAEEGRVAVRNIRRHSKTDMESMHGEISDDDIRRGEDELQK
                     LTDRCVDHIDRLLGHKEEELLEV"
     gene            complement(856..1557)
                     /gene="pyrH"
                     /locus_tag="VFU96_12130"
     CDS             complement(856..1557)
                     /gene="pyrH"
                     /locus_tag="VFU96_12130"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU5114057.1"
                     /translation="MRRVVLKLSGEAFADPGLGYGIDPLTVVRVAEEIAEAHSDDIQI
                     AVVVGGGNIFRGVSQAAKGMDPASADYMGMLATVINALALRDALEKAGVPTRVQSAIT
                     MQELAEPYIRLRAIRHLEKGRVVIFAAGTGNPFFTTDTTAALRAVELGADALLKASKV
                     DGIYDADPATHPDAKRLDELSYMDFISDELRVMDTTAVTMSKEHGVPIVVFDMTTPGN
                     IVKAVSGEKIGTVIH"
     gene            complement(1581..2192)
                     /gene="tsf"
                     /locus_tag="VFU96_12135"
     CDS             complement(1581..2192)
                     /gene="tsf"
                     /locus_tag="VFU96_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008336547.1"
                     /note="EF-Ts; functions during elongation stage of protein
                     translation; forms a dimer; associates with EF-Tu-GDP
                     complex and promotes exchange of GDP to GTP resulting in
                     regeneration of the active form of EF-Tu; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEU5114058.1"
                     /translation="MPEFGAKDIQKLRQDAGVGMMDAKKALSDADGDLELAFELLRER
                     GQAKMAKRTDREANEGAIGSYIHIQNERPVVGVLVELACETDFVAKSAEFRETASDIA
                     MHVSWGKPMWVTRDEVDQDALAKERELISRQALAEGKPENVVDRIVDGKIESWFAENV
                     LYDQKFVNAEKFDGTVGDMVANLASKMGENISVRRLSRIAVGE"
     gene            complement(2185..3024)
                     /gene="rpsB"
                     /locus_tag="VFU96_12140"
     CDS             complement(2185..3024)
                     /gene="rpsB"
                     /locus_tag="VFU96_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013284605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU5114059.1"
                     /translation="MKQLLEAGVHFGHQTRRWNPKMRPYIYGERNGIHIIDLRQTVDQ
                     TAAAAEYVRTLTSDGGVVLFVGTKKQAQAAVAEHATRSGMPYVNYRWLGGMLTNFQTI
                     QKRIFYMRELRRLEASGEINALPKKERLKLRRELAKLEQNLGGVVDLQKTPDAVFIVD
                     VNTEATAVTEAARLGMPVIALVDSNSDPDQVDYVIAGNDDAIRAADLIAGAIADAAIE
                     GRRMAGAKASKGDAAGDEGSTSEAEDFEPTVDESIPDFEQEPAPATVASAEAAAKPEA
                     ENA"
     gene            complement(3195..3500)
                     /locus_tag="VFU96_12145"
     CDS             complement(3195..3500)
                     /locus_tag="VFU96_12145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5114060.1"
                     /translation="MAALDEFVGNESSTSVGEMLADPSGVSPEMAFQSEETRRGLVDA
                     INRLPGRERLVITLYYYEGMTLAEIGDVLGVTESRVCQIHAKTMMSLRNRLGEPSWG"
     gene            complement(3574..3674)
                     /locus_tag="VFU96_12150"
                     /pseudo
     CDS             complement(3574..3674)
                     /locus_tag="VFU96_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003962024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
BASE COUNT          587 a         1187 c         1146 g          754 t
ORIGIN      
        1 ccttggtact cctgggtggt ggacccggtg aactggtttg cctcgaagtc gtcgagatcc
       61 tgggggaagc tcatcccctc gtagtcggcg tcgcccggcc ccggttttga gctcgactca
      121 tcgtccgccc cctccgctga gtcgtcggca tcggagtcat cgggagccgc atccccctct
      181 gcaagctcat cctcgagtcg tttgggaagg gcgaatacct cggcgtcatc gtcgccgtca
      241 tcccagagac ggctcggctg cttctcttcg tcgggggttc gctcgctcaa atcacacctc
      301 gaggagctct tcttccttgt gaccgaggag acgatcgatg tgatccacgc accggtcggt
      361 cagcttctgc agctcatctt cgccgcggcg gatgtcgtcg tctgagatct cgccgtgcat
      421 cgattccata tcggtcttgg aatggcggcg gatgttacgg acggcgaccc gtccctcctc
      481 tgccatgttc cgcaccacct tgatcaggtc cttgcgcctt tcctcggtga gaggcgggaa
      541 ggcgatgcgg atcacatttc cgtcgttcgt cgggttgagg ctgagctcag acgtctgcag
      601 ggctcgttcg atgtcaccga cggtcgactt gtcgaatggc tggaccacga gcagacgagg
      661 ctcgggcacc gagatcgatg ccagttgctg aagaggtgtc ggcgacccgt agtactcgac
      721 ggttatccgc tggagaagct gaggattcgc ccgcccggtg cggacagtcg agaactcgga
      781 ggccacatgg tcgatcgcct ggtccatctt gtgctcggcc tcggtcagga ggtcactgat
      841 cactgttcct cctcgtcagt gaatcacggt gccgatcttc tcaccgctca ccgccttgac
      901 gatgttgcca ggtgttgtca tgtcgaacac gacaatcggc acaccgtgct ccttgctcat
      961 cgtaactgcg gtggtgtcca tgaccctgag ctcatcggag atgaagtcca tataggacag
     1021 ctcgtcgagg cgcttggcgt cggggtgggt ggcagggtcg gcgtcgtaga tgccatccac
     1081 cttgctcgcc ttgaggagtg cgtcggcgcc gagctcgacg gcccggagtg ccgccgtcgt
     1141 gtcggtggtg aagaacggat tgccggtgcc cgcggcgaat atcaccactc gtcccttctc
     1201 gaggtggcga atggcgcgga gacggatgta gggctcggcc agctcctgca tcgtgattgc
     1261 gctctgcacc cgggtgggca cacctgcctt ctccagcgcg tcccggaggg cgagggcgtt
     1321 gatcacggtg gccagcatcc ccatgtagtc ggcagaggct ggatccatgc ccttggctgc
     1381 ctggctcact ccgcggaaga tgttgcctcc accgacgacc accgcgatct ggatgtcgtc
     1441 ggaatgggcc tcggctatct cctccgccac cctgaccacg gtcaggggat cgatgccgta
     1501 accaagcccg ggatcggcga aggcctcacc ggacagcttg aggacgactc ttctcaccgg
     1561 gggattggac aattcgcctc ctactcgccg acggcgatgc gggacagtct gcgcaccgag
     1621 atgttctcac ccatcttgga cgcgagattg gccaccatgt cgccgacagt gccgtcgaac
     1681 ttctccgcgt tgacgaattt ctggtcgtag aggacgttct cggcgaacca cgactcgatc
     1741 ttcccatcga cgatccgatc gacgacgttc tccggcttgc cctcagcgag tgcctgacgg
     1801 gagatgagct cacgctcctt ggccagggca tcctgatcca cctcgtcgcg ggtcacccac
     1861 atcggctttc cccagctgac gtgcatggcg atgtcgctcg ccgtctcgcg gaactcggcg
     1921 ctcttggcga cgaagtcggt ctcacaggcc agctcgacca ggactcccac caccggtcgc
     1981 tcgttctgga tgtggatgta gctgccgatg gctccctcgt tggcctccct atcggtgcgt
     2041 ttggccatct tggcctgccc ccgctcccgg agcagctcga acgccaactc caggtcccca
     2101 tctgcgtcgc tcaatgcctt cttggcatcc atcatcccga cgccggcgtc ctgcctcagc
     2161 ttctggatgt ccttggcacc gaactcaggc attttcggcc tcgggcttgg ccgcggcctc
     2221 agccgatgcg acagtcgcgg gtgccggctc ctgctcgaag tcaggtatcg actcgtcgac
     2281 cgtcggctcg aagtcttccg cctcggaggt cgagccctca tcgccggcgg cgtcgccctt
     2341 ggaagccttg gcaccagcca tcctccgacc ctcgatggct gcgtcggcga tcgctccggc
     2401 gatgagatcg gcggcacgga tcgcgtcgtc gttaccggcg atcacgtagt ccacctgatc
     2461 gggatcgctg ttcgagtcga cgagggcgat caccggcatc ccgagacggg ccgcttcggt
     2521 gaccgcagtc gcctcggtgt tgacgtcgac gatgaaaacg gcgtccggag tcttctgaag
     2581 gtcgaccact ccaccgaggt tctgctccag cttggccagc tcgcgccgca gcttcaggcg
     2641 ctccttcttg ggaagggcgt tgatctcacc gctggcctcg agacgacgca gctcacgcat
     2701 gtagaagatg cgcttttgga tggtttggaa gttggtcagc atcccgccga gccagcggta
     2761 gttgacatag ggcatgcccg accgggtggc atgctcggcc acagccgcct gggcctgctt
     2821 cttggtgccg acgaacagaa cgacgccccc atcagaggtg agggtccgaa cgtattcggc
     2881 ggcggcggcg gtctggtcga ccgtctgccg gaggtcgatg atgtggattc cgttgcgctc
     2941 cccgtagata taggggcgca tcttcgggtt ccagcgccga gtctgatgcc cgaaatggac
     3001 accggcctcc aagagttgct tcatcgagac agccaaagct gctcctttct ggttgttcct
     3061 ccgcctgccg gcgattgcct acccgtttcc acggatgacc ctcttcggga cgcgcccgac
     3121 cagtgagggt cagcagacgt gtgtgatagg gagatggtaa gggcagacgg cgcagaacgt
     3181 aaaacgtcga gcgcttaacc ccaggagggc tctccgagcc gattgcggag ggacatcatc
     3241 gtcttggcgt ggatctggca gacccgtgac tcggtgacgc cgagcacgtc accgatctcg
     3301 gccagtgtca tcccctcgta gtagtagagc gtgatcacga gtcgctcccg cccgggcagg
     3361 cggttgatgg cgtcgaccag cccgcggcgg gtctcttcgg actggaacgc catctcggga
     3421 gagaccccgg aggggtcggc gagcatctca cccacagagg tagacgactc gttgcccacg
     3481 aactcgtcca gggcggccat ccccgaacgc ccgatctcgg cgagcgcgct ctgcaggtct
     3541 ccttccgcgc cctcgtgcgt gacgacatgg agtgtcgccg agccgattgc gcagggacat
     3601 catggtcttg gcgtggatct gacagacccg tgactcggtg acgccgagca cgtcaccgat
     3661 ctcggccagt gtca
//
LOCUS       DASTXO010000570         2318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_919301, whole genome shotgun sequence.
ACCESSION   DASTXO010000570 DASTXO010000000
VERSION     DASTXO010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919301"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VFU96_12155"
     CDS             <1..346
                     /locus_tag="VFU96_12155"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5114061.1"
                     /translation="WDALPADLKEIVKTSVQASYWNHFVRFQEKTAKACQELLAAGVK
                     IIKTTDELNKGFLRAYDEVWQADAAKDEFYKKVVDSQKKYSGLVVPYRLSYWPNYNFI
                     AEHYYKDKIWLK"
     gene            490..1074
                     /locus_tag="VFU96_12160"
     CDS             490..1074
                     /locus_tag="VFU96_12160"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEU5114062.1"
                     /translation="MATAAPVEPNHRDAAPAFRTTVELIDRFASYTGRATAWLVIPMT
                     IAVTWEVVARHFFRAPTMWAFDVTYMLYGTHFMLGTAYTLMRIGHVRTDMLYSRWSIR
                     RQNRIDAIGYFFFFFPAMIFLFYFGWQEAYHSFQIGETSDASPWRPIVYPFKFVIPLT
                     ALFLLIQGIAELLKSLYAIRTGREWVHREHLEIG"
     gene            1071..>2318
                     /locus_tag="VFU96_12165"
     CDS             1071..>2318
                     /locus_tag="VFU96_12165"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEU5114063.1"
                     /translation="MSPELLGGIMLVVMLGAIFIGFPISFTLLLLALIFGWVGLGDRA
                     FYIMVFQTIGLMKEEVLAAVPLFIFMGDLMEQGGLMERLFRGFQLLMGPVPGSLYVGV
                     LITATIFGIAAGTVGATVVLMGIMAVPIMTKAGYDVKMSAGAITAGGTLGILVPPSVM
                     LVVMGPVMGVSVAELYASCFGPGFLLAGIFIAYCVIRSLINPRLGPPLPPEERTGSLL
                     EVAKELTLGMLPHAILIIATLGTIIWGWATPTEAAAMGVVGAAFLAVVYRQFTWQRLK
                     DAVYNTLLQSSTVIFLAVCGNVFGAVFSRLGTATVATNALLAWQIPPVAMLTVIMVVI
                     FLLGWPFEWPAVILVFVPLMQPIIASMKIDMLWFATLVAVNLQTAFLSPPVAMSAYYL
                     KGVAPNWNLGDIYKGMGQFMVLQL"
BASE COUNT          369 a          766 c          723 g          460 t
ORIGIN      
        1 gtgggacgcg ctgccggccg acctgaagga gatcgtcaag acctcggtcc aggcctcgta
       61 ctggaaccac ttcgtgaggt tccaggagaa gacggccaag gcctgccagg aactgctcgc
      121 tgccggcgtc aagatcatca agacgacgga cgagctgaac aaggggttcc tgcgcgcgta
      181 cgacgaggtc tggcaggcgg acgcagccaa ggacgagttc tacaagaagg tggtcgactc
      241 gcagaagaag tactccgggc tggtggtgcc ctaccgcctc tcgtactggc caaactacaa
      301 cttcatcgcc gagcactact acaaggacaa gatctggctg aagtagacga ccggtgaggc
      361 tctgctccgg acggcgggcc gattcgcggg cccgccgtcc gggcgttcga cgaactgctt
      421 cgccgaccca cggatccgct cttccttgcg ctcggcgctc gattcccgcg aactgaacgg
      481 aggactcgtg tggcgactgc ggcaccggta gaacccaacc accgcgacgc ggcgccggcc
      541 ttccggacca cggtcgagct gatcgaccgc ttcgcgagct acacgggtcg ggcgaccgcg
      601 tggctcgtca tcccgatgac catcgccgtc acctgggagg tcgtggcccg ccacttcttc
      661 cgcgctccca cgatgtgggc cttcgacgtg acgtacatgc tgtacggcac gcacttcatg
      721 ctgggaaccg cttacaccct catgcggatc gggcacgtgc gcaccgacat gctctattcg
      781 cgctggtcca tccgccgcca gaatcgaatc gacgcgatcg ggtacttctt cttcttcttc
      841 ccggcgatga tcttcctgtt ctacttcggc tggcaggagg cctatcactc gttccagatc
      901 ggcgagacct cggacgcgag cccctggcgc ccgatcgtct acccgttcaa gttcgtcatc
      961 ccgctcaccg cactgtttct cctcatccag ggcatcgcgg agctcctcaa gagcctctac
     1021 gccattcgca cgggccgcga gtgggtccat cgtgagcacc tggagatcgg atgagtcccg
     1081 agctgctcgg cggcatcatg ctggtcgtca tgctcggggc gatcttcatc ggcttcccga
     1141 tctcgttcac gctgctgctc ctcgcgttga tcttcggctg ggtcgggctc ggggaccggg
     1201 cgttctacat catggtgttc cagacgatcg gcctcatgaa agaagaggtg ctggcggcgg
     1261 tgccgttatt catcttcatg ggcgacctga tggagcaggg cggcctcatg gagcgactgt
     1321 tccggggctt tcagctcctc atggggccgg tgcccggctc gctctacgtg ggcgtgctca
     1381 tcaccgcgac aattttcggg atcgccgccg gcacggtggg ggcgacggtg gtgctcatgg
     1441 gcatcatggc ggtgcccatc atgaccaagg ccggctacga cgtcaagatg tccgctgggg
     1501 ccatcaccgc gggcggcaca ctgggcatcc tggtccctcc gagcgtcatg ctggtcgtga
     1561 tggggccggt catgggcgtc tcggtggccg agctctacgc ctcgtgcttc ggtcccggct
     1621 tcctcctggc cggtatcttc atcgcctact gtgtaatccg cagcctcatc aacccgaggc
     1681 tggggccgcc actgccgccc gaggagcgaa ccgggagtct cttggaggtc gccaaggaac
     1741 tgacgcttgg catgctgccg cacgccatcc tcatcatcgc caccctgggg accatcatct
     1801 ggggctgggc aacaccaacc gaggcggcgg ccatgggcgt cgtcggcgcc gcgttcctgg
     1861 cggtcgtgta ccggcagttc acgtggcagc ggctcaagga cgcggtctac aacaccctgc
     1921 tccagtccag caccgtcatc ttcctggctg tctgcggcaa cgtgttcggc gcagtgttct
     1981 cgcggctcgg aacggcgacg gtggccacca atgcgctgct cgcctggcag atccctccgg
     2041 tggcgatgct gaccgtcatc atggtcgtca tcttcctgct cggctggccc tttgaatggc
     2101 cggcggtcat cctcgtcttc gtaccgctca tgcagccgat catcgcctcg atgaagatcg
     2161 acatgctgtg gttcgcgact ctggtcgcgg tgaacctcca gacagcgttc ctctccccgc
     2221 cggtcgcgat gtctgcgtac tacctcaagg gcgtggcgcc gaactggaac ctcggggaca
     2281 tctacaaggg gatggggcag ttcatggtgc tccagctc
//
LOCUS       DASTXO010000571         2012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1730200, whole genome shotgun sequence.
ACCESSION   DASTXO010000571 DASTXO010000000
VERSION     DASTXO010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1730200"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VFU96_12170"
     CDS             complement(<1..572)
                     /locus_tag="VFU96_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013016058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEU5114064.1"
                     /translation="MEQNRRTTSDIEIRVVHEEPGDPASIGHPGEHPFTRGPHPTMYR
                     GRLWTMRQYAGFGTADETNRRFRALLDAGQTGLSVAFDLPTQMGLDSDDPLAAGEVGK
                     VGVAIDTLDDMRRLFTGIPLDQVSTSMTINSTAAILLLLYQLVAEEQGVEPGQLRGTI
                     QNDILKEYAARGTYIYPPAPSLRLVTDVIE"
     gene            650..>2012
                     /locus_tag="VFU96_12175"
     CDS             650..>2012
                     /locus_tag="VFU96_12175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEU5114065.1"
                     /translation="MARKALIHQIEARERRGRWFGALATLISISLLLSGWVGLFAFLG
                     ANSAYGTFEGVKNEWVPDTDSMELTLPDLSRVSRIYADSGEVLAELHDGRNSEPVAYK
                     DVPETLVYAILAAEDSEFFEHEGIDFSAIVSAAIDNLITDRTRGGSTITQQVVKNAFV
                     GSEVSIQRKITEAFVSAEVERRFPKERILEFYMNSVYFGAGAYGVKTASEEFFNKPMD
                     QLTIAESATLAVMVRNPSLYNPRNRPELTLERRDEVIREMQENGWVTEAQARNAADAP
                     IGVIEAPLRRGQADHVVAEVKRQLLNDPEFDFLGASPEERKKAVFGCPADDVACEGGG
                     GLQVYTTIDLELQKKANEILAAWLPLPPYEENLAKCREILTEETDDFLARYAETHSCV
                     PTGAMTMTDNWSGAVKVMASGLPFEFSQFDLAVQGRRNPGSSFKPFGLIAALENGFTM
                     GHSW"
BASE COUNT          373 a          656 c          619 g          364 t
ORIGIN      
        1 aactcgatga cgtcggtgac cagtcgaagc gacggagccg gcgggtagat gtaggtgccg
       61 cgagcggcgt actccttgag gatgtcgttt tggatcgtcc cccggagctg acccggctcg
      121 acgccctgtt cttcggccac caactggtac aggagcagga ggatggctgc agtggaattg
      181 atcgtcatcg acgtcgacac ctgatcgagt gggattccgg tgaacagtct ccgcatatcg
      241 tcgagggtgt cgatggcgac cccgaccttt cccacctccc ccgccgccag cggatcgtca
      301 gagtccagac ccatctgtgt ggggagatcg aacgcgacgc tcaacccggt ttgaccggca
      361 tcgagcaggg cccggaaccg ccggttcgtc tcgtccgccg tgccgaagcc cgcgtactgg
      421 cgcatggtcc agagacggcc ccggtacatg gtcggatgcg gccctcgggt gaaggggtgc
      481 tccccgggat gaccgatcga agcggggtct cctggctcct cgtgcaccac ccgaatctcg
      541 atgtcgctgg tagtgcgacg gttttgctcc atgttccggg tatccttgca tcgttcctgt
      601 cggtgtaggt cgggaactcc caggacgagt acgacgtccc tctctctcaa tggccagaaa
      661 agcgctgatc caccaaatcg aggcgcggga acggcgcggc cgatggttcg gcgcccttgc
      721 cacgctcatc tcgatctccc tgctcctctc cggatgggtg gggctcttcg cattcctcgg
      781 cgccaattcg gcttacggca cattcgaggg cgtcaagaac gaatgggtgc ccgacaccga
      841 ttcgatggag ctcacgcttc ccgacctgag ccgggtcagc cggatctacg ccgactcggg
      901 tgaagtcctc gccgagttgc acgacggtcg caattccgag cccgttgcct acaaagacgt
      961 gcccgagacc ctggtctacg ccatcctcgc cgccgaggac agcgaattct tcgagcacga
     1021 gggaatcgat ttctctgcca tcgtgtccgc cgccatcgac aacctgatca ccgaccgcac
     1081 ccgtggtggc tcgacgatca cccaacaggt cgtcaagaac gcctttgtgg gtagcgaggt
     1141 cagcatccag cgcaagatca cagaggcgtt cgtctctgcg gaggtcgagc gacggttccc
     1201 caaggagcgg atcctcgagt tctacatgaa ctcggtgtat ttcggggcgg gcgcctacgg
     1261 ggtcaagacc gcatccgaag agttcttcaa caagccgatg gaccagctca cgatcgccga
     1321 gtcggcaacg ctggcagtca tggtccgcaa cccttccctc tacaacccgc gcaaccgccc
     1381 ggagctcact ctggaacgac gggacgaggt gatccgggag atgcaggaga acgggtgggt
     1441 gaccgaggcc caagcccgta acgctgcaga cgcgccgatc ggtgtgatcg aagctcccct
     1501 tcgccgcgga caggctgacc atgtagtcgc cgaggtcaag cggcagttgc tcaacgaccc
     1561 cgagttcgac ttcctgggag cctcccccga ggagcggaag aaggccgtgt ttggctgtcc
     1621 ggccgacgac gtcgcttgtg agggaggggg cggtctccag gtctacacca ccatcgacct
     1681 cgagctgcag aaaaaggcga acgagatcct ggccgcctgg ctccccctgc cgccatacga
     1741 agagaacctc gccaagtgcc gcgagatcct caccgaggag acggacgact tcctcgcccg
     1801 gtacgccgaa acccactcgt gtgtccctac cggggccatg accatgaccg acaactggag
     1861 cggcgcggtg aaggtgatgg catcgggcct ccccttcgag ttctcccagt tcgacctcgc
     1921 cgtgcagggc cgtcgcaacc ccggatcgtc tttcaaacct ttcggcctca tcgcggccct
     1981 cgagaacggc ttcaccatgg gtcatagctg ga
//
LOCUS       DASTXO010000572         2811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_772501, whole genome shotgun sequence.
ACCESSION   DASTXO010000572 DASTXO010000000
VERSION     DASTXO010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772501"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            288..1688
                     /locus_tag="VFU96_12180"
     CDS             288..1688
                     /locus_tag="VFU96_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012999137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5114066.1"
                     /translation="MTDRILISGGIVLTQDSELGELPNADVLIEDGKIAAIGEDLPAD
                     DARVIDAAGDIVIPGFIDTHRHTWETCIRTCAPDFALITYFSAILDKFAPHYRPEDVH
                     SATQWGALECINAGITTLVDWAHIMNTPDHADASVAALKETGIRSVFAYGFPNTSLVD
                     WWFGPDYTGSVLTSDGDEARRLRSQYFTSDDDRITMGLATRGPGFCKPDVVRHDWELA
                     KELGINITVHVAMDRFGYTKGQITALRDMDLLYPGTTYVHASHFSDEEWQLVSDSGGN
                     VSFAPQIEIQMGHGWAPAVTAVEYNVPIGLSSDVATTASADQFTQMHAVFGSERGRKH
                     QDAWDDDLDGLEASPGLISAREALSWATIGGAEVAGISDRTGSLTPGKKADIVIIDGS
                     AVNVAPIIDPVGAVVCAADVSNVKTVLVDGEVLKEDFKLKASLDAPRRAVEESRDYLL
                     EMFGDPEPGWLPTGRR"
     gene            complement(1700..>2811)
                     /locus_tag="VFU96_12185"
     CDS             complement(1700..>2811)
                     /locus_tag="VFU96_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU5114067.1"
                     /translation="PPRAEAKAAIAKCHDAGIRVRMITGDHATTAAAIAGELGIEGRA
                     LTGAQFAALSDDQLIEQLPEIGVIARVAPEDKVRLVRMLQQSQNVVAMTGDGVNDAPA
                     LKAADIGVAMGITGTEVSKEAAVMILTDDNFATIVGAVEYGRALYDNLLKYLRFQMST
                     LVAYIAVFIGAGIFAIAGGIPLDPLQILWLNMVIDIPLAIALGFDEPTPGLMQRAPRP
                     VGAPVLSSRDWVRLCVQGFVMTVGTLAVYLLAEPQYGGAVASTMLLTTLSLFHVVAGY
                     MARDPEGTVFNREALPGATQLKRYGLALLAIILVTMLGFLQRIFSTVELGFNQWAICI
                     GIALSLLVVEELIKLFLRRGVAARVSAPAQPTTAV"
BASE COUNT          536 a          891 c          910 g          474 t
ORIGIN      
        1 cgacgcgagc catgcggcgc ttgcgtctgc gggcgctcct cgcctttcga cttcggtcga
       61 ccgccatcac aacagcgtat gcgtgcgggg tggagggggc tgtcatctcg cagttggctc
      121 atgccggttc cggcgggctc ctcggtgaaa tagctcggcc tggcggcgtt acgatcctga
      181 ccgaggtgcg aggtcgagtg ttcgatggac acatctgcgc atcggcaagt ccatgacacg
      241 gctgcgccgg ccggcggggc cgatcaccag ggagaaaggc cacagctgtg actgaccgaa
      301 tcctcataag cggcggaatc gtcctgaccc aagactctga gctcggtgaa ctgcccaatg
      361 ccgacgtgct gatcgaggac ggaaagatcg cagccatcgg cgaagacctg cctgccgacg
      421 atgccagggt catcgatgcg gccggtgaca tcgtcatccc cggattcatc gacactcacc
      481 ggcacacgtg ggagacgtgc atccggactt gtgcacccga cttcgcactg atcacctact
      541 tcagcgcgat cctcgacaag ttcgccccgc actaccggcc ggaagacgtc cactcggcga
      601 cacagtgggg cgcgctcgag tgcatcaacg cagggatcac gacgctcgtc gactgggctc
      661 acatcatgaa cacgcccgat cacgccgacg cctcggttgc cgctctcaag gagacaggca
      721 ttcgatcggt attcgcgtat ggcttcccca acacctcact ggtggactgg tggttcggcc
      781 ccgactacac cggcagtgtg ctgacctcgg atggagacga ggcgcgccgc ctgcggtcgc
      841 agtacttcac gtccgatgac gaccggatca ccatgggact cgccacgagg ggacccggct
      901 tctgcaagcc cgatgtggtg cgtcatgact gggagctggc caaagagctc ggaatcaaca
      961 tcacggtgca cgtggccatg gaccggttcg ggtacacgaa gggccagatc actgccctgc
     1021 gtgacatgga cctcctctat cccggcacga cctacgtcca cgcctcccac ttctctgacg
     1081 aagagtggca actcgtctcc gattcggggg gcaatgtcag cttcgccccc cagatcgaga
     1141 tccagatggg acacggatgg gcaccagcgg tcaccgcagt cgaatacaac gtgccgatcg
     1201 ggctttcgtc ggacgtcgcc acgaccgctt cggccgatca gttcacccag atgcacgcag
     1261 tcttcgggtc agagcgcggg cgcaagcacc aggacgcgtg ggacgacgac ctcgatggtc
     1321 tggaggcctc tcccggtctc atcagcgcgc gagaggccct ttcgtgggcc acgatcggag
     1381 gagccgaggt ggcagggatc tcggatcgca ccggatcgct cacgccgggg aagaaggcag
     1441 acatcgtcat catcgacgga tctgcggtga atgtggcgcc gatcatcgat ccggtcggcg
     1501 cagttgtctg cgcggcggac gtctcgaatg tgaagaccgt cctcgtcgat ggcgaggtgc
     1561 tcaaggaaga cttcaagctc aaggcgtccc tagatgcgcc gcgccgggcg gtcgaggaat
     1621 cgagggacta cctgctcgaa atgttcggcg atcccgagcc agggtggctc ccaaccggcc
     1681 gcaggtgagg tgattcggtt cagaccgccg tcgtcggctg cgcaggcgcc gaaaccctcg
     1741 cagcgacccc gcgacgcagg aacaatttga tcagttcttc gaccacgagc aagctcaacg
     1801 cgatcccgat gcagatcgcc cactggttga agcccagctc gaccgtcgag aagatgcgct
     1861 ggagaaagcc gagcatggtc accaggatga tggccagaag ggccaacccg tagcgcttca
     1921 gctgagtggc gccgggtaga gcctcccggt tgaagacggt gccctccggg tcccgagcca
     1981 tgtagccggc gaccacatgg aagagggaca gcgtcgtcaa gagcatggtc gaggcaacgg
     2041 cgcctccata ctgcggctcg gccagcaggt agacggcgag ggtcccgact gtcatgacaa
     2101 acccctgtac acagagccgc acccagtctc gagaggacag gacgggcgca cccaccggtc
     2161 tcggagcacg ttgcatcaaa ccgggggtgg gttcgtcgaa gcccagggcg attgcgagcg
     2221 ggatgtcgat caccatgttc agccacagga tctggagcgg gtcgagcggg atgccgccgg
     2281 cgatggcaaa gatgccggcg ccgatgaaga ccgcgatgta ggcgacaagg gtcgacatct
     2341 gaaaacgaag gtatttgaga agattgtcgt agagggcacg cccgtactcg accgctccga
     2401 cgatcgtggc gaagttgtcg tcggtgagga tcatcaccgc cgcctccttc gagacctcgg
     2461 tgccagtgat ccccatggcc acgccgatgt cagccgcctt cagcgccggg gcgtcgttga
     2521 ccccgtctcc ggtcatggcc acgacgtttt gggactgctg cagcatacgg accagacgca
     2581 ccttgtcttc gggagcaacc cgagcgatca ccccgatctc cggcagttgt tcgatcagct
     2641 ggtcgtcgct caacgcggcg aactgggcgc ctgtgagcgc cctgccttcg atgccgagct
     2701 ccccggcgat cgctgcggcg gtggtggcat ggtcgcctgt gatcatccgc acccggatcc
     2761 cagcgtcatg gcacttggcg atggcggcct tggcctcggc gcggggggga t
//
LOCUS       DASTXO010000573         2457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_256232, whole genome shotgun sequence.
ACCESSION   DASTXO010000573 DASTXO010000000
VERSION     DASTXO010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256232"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VFU96_12190"
     CDS             <1..275
                     /locus_tag="VFU96_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5114068.1"
                     /translation="ILGLWPAVFPFVVVTLALASALTRSTGLGSLVTLGALFAGAVAW
                     ERTGLDNPWGLGDPTLLMVMVIGLGVIISPRHWRDARNRLTSPVRP"
     gene            complement(253..708)
                     /gene="dtd"
                     /locus_tag="VFU96_12195"
     CDS             complement(253..708)
                     /gene="dtd"
                     /locus_tag="VFU96_12195"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEU5114069.1"
                     /translation="MRIVVQRVSRASVDVEGAEVGSIDDGLLLLVGIEPFDTEKDIVR
                     GAEKVANLRVFADDEGQMNRSLVEVGGSALVVSQFTLLAEVRKGRRPSFVGAAPPELA
                     GPMVDRFVDVLRGTGTHVETGVFGASMDVELVNSGPVTLVLEVRDGRVT"
     gene            744..>2457
                     /locus_tag="VFU96_12200"
     CDS             744..>2457
                     /locus_tag="VFU96_12200"
                     /inference="COORDINATES: protein motif:HMM:NF039580.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU5114070.1"
                     /translation="MARKSADPPALMTGPAGSRVVYLLDAASDYEAALLARWAREETG
                     GEVETVRITSSRRGRGGEIDELAEELAAPDDPFLIPLRVLWLAPLKKGRRAVSWWDVL
                     RPGDARDPRGLRARWIRMARPGRMRLTAAAGAHASDMAEDYQTSGQVDPLTSFVTRRA
                     WLILDRAERRLVGNRYKIPRFVPEAILSRREFLEAVDAVAAETAMSPALAREKAERYL
                     REIAATHSPYVIDLIANGIHKLYSQGYGDIKYSEDDVRRLAALGDGNPIIFLPAHRSN
                     LDRLSLQFMLWENDFPPNHTAGGINLNFFPVGPLVRRTGVFFIRRSFKDNPLYRMVLR
                     SYLDYLVEKRFPLEWYMEGGRSRSGKLMPPRLGLLSYVVDSMHRGKADDLQLVPVSIA
                     YDQIQDVPDYAREAQGKDKEKESLGWMLKAVRSLRRRYGDIHIRFGEPVSARSMVGPM
                     DRGEETSVGLQKLAFEVMYRIGHVTPITPTALVSVALLAARGTARSSDQLADEAARLV
                     EFARARGLPTTEDADLRDPARVAVILDWLAEHDNVSSHEALGRRVFWLDEDQMIRISY
                     YRNMI"
BASE COUNT          428 a          828 c          771 g          430 t
ORIGIN      
        1 tgatcctcgg gctctggccg gcggtattcc ccttcgtcgt ggtaaccctc gccctcgcat
       61 cagcgctgac gaggtcgaca ggtctcggga gcctggtgac actgggcgca ctcttcgcgg
      121 gggcagtcgc ttgggagaga accggcctcg acaacccttg gggtctcggc gaccccactc
      181 tcctcatggt gatggtgatc ggactcggcg tgatcatcag cccacggcat tggcgggatg
      241 cccgcaaccg gctcacgtca cccgtccgtc cctgacctcc aacacaaggg tcaccgggcc
      301 ggagttgacc agctcgacgt ccatcgacgc gccgaagacg cccgtctcga cgtgagtgcc
      361 ggtgccgcga agcacgtcca cgaagcgatc gaccatcggc ccggccagct cgggtggggc
      421 ggccccgacg aacgacgggc gtcgaccctt gcggacctcg gccagaagtg tgaactggct
      481 cacgaccaaa gccgatccgc cgacctcgac cagcgaccgg ttcatctgcc cttcgtcatc
      541 ggcgaacact ctgaggttgg ccaccttctc cgcgccgcgc actatgtcct tctccgtgtc
      601 gaacggctcg atgccgacga ggagaaggag cccgtcatcg atcgatccga cctctgcccc
      661 ctcgacatcg accgatgccc gactgacgcg ttgcaccacg atccgcacgg gaggcgattg
      721 tggcagccca atagagtggt gctgtggcga ggaaatcggc cgatccccca gctttgatga
      781 caggcccggc cggttcacgg gtcgtctacc tgcttgatgc cgcttccgac tacgaggccg
      841 ctctcctcgc acgttgggcc cgtgaggaga ctggcggcga agtcgagacg gtcaggatca
      901 catcgtcgcg gcgcggcagg ggtggtgaga tcgacgagct ggccgaagaa ctggcggccc
      961 ccgacgaccc cttcctcata ccgctccgcg tcctctggct ggccccactg aagaaaggtc
     1021 gcagggccgt cagctggtgg gatgtgctgc gaccgggcga cgcccgggat cccagaggac
     1081 tgcgggccag gtggattcgc atggcccggc cggggcgcat gaggctcacc gccgccgccg
     1141 gcgcgcacgc atcggatatg gccgaggact atcagacgtc gggccaggta gatccgctca
     1201 ccagctttgt gacgaggcga gcgtggctca ttctggaccg ggctgagcgc cggctggtag
     1261 gcaaccgcta caagatccct cgattcgtgc ctgaggcgat cctctcccgc cgggagttcc
     1321 tcgaggccgt cgacgcggtc gccgcggaga ccgccatgtc tcccgccctg gcccgcgaga
     1381 aggccgagcg gtatctccgt gagatcgcag ccactcactc gccctacgtg atcgacctca
     1441 tcgccaacgg gattcacaag ctgtacagcc agggctatgg cgacatcaaa tactccgagg
     1501 acgatgtccg acgtctggcc gctctcggcg atggaaatcc gatcatcttc ctgcccgccc
     1561 accgatccaa cctcgaccgt ctcagtcttc agttcatgtt gtgggagaac gacttcccac
     1621 caaaccacac cgctggtggc atcaacctca atttcttccc ggtggggccc ctggttcggc
     1681 ggaccggcgt gttcttcatt cgtcgctcct tcaaggacaa ccccctgtac cggatggtcc
     1741 tgcgctcata tctggactat ctcgtcgaga agcgattccc actcgagtgg tacatggaag
     1801 gggggcgatc gcgctcggga aagctgatgc cgccccgtct cgggctcctc agctacgtgg
     1861 tcgactccat gcaccggggc aaggccgacg acctccaact ggtcccggtt tcgatcgcat
     1921 acgaccaaat ccaggatgtt ccggattacg cccgcgaggc ccagggcaag gacaaggaaa
     1981 aggaatccct cggctggatg ctgaaagctg tgcgctctct gcgtcgacgg tacggagaca
     2041 tccatatccg cttcggggag cccgtgtcgg cgaggagcat ggtcggcccg atggatcggg
     2101 gcgaggaaac ttcagtcggg ctgcagaaac tggcgttcga ggtcatgtac cggataggcc
     2161 atgtcactcc gatcaccccc acggccctgg tgagtgtcgc cttgctcgcg gcgcgaggga
     2221 ctgcccggtc ttcggatcag ctcgccgatg aggcggcgcg gctggtggag ttcgcccggg
     2281 cccgcggcct tcccactacc gaggatgcag acctccgcga cccggcacgg gtggccgtca
     2341 tcctcgactg gctcgccgag cacgacaacg tgagcagcca cgaggcgctg ggaagacgtg
     2401 tcttctggct cgatgaggac cagatgatcc gcatctccta ctaccgcaac atgatcg
//
LOCUS       DASTXO010000574         3154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_625248, whole genome shotgun sequence.
ACCESSION   DASTXO010000574 DASTXO010000000
VERSION     DASTXO010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625248"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VFU96_12205"
     CDS             complement(<1..328)
                     /locus_tag="VFU96_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5114071.1"
                     /translation="MAQTTEPIEGWARAGRWALLGVLLLIPLGLATVQLTLGDRALGD
                     DILGAVGFLIYAAIGSLIVVRRNGHPTGWLLLLIGLAVLFADGLAGLPGVSELLADWV
                     ASWGWGL"
     gene            complement(427..819)
                     /locus_tag="VFU96_12210"
     CDS             complement(427..819)
                     /locus_tag="VFU96_12210"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU5114072.1"
                     /translation="MRRLQSRPGEERSIMPTANVTMNVREAAPRVRVIDIEGEITGFS
                     EKEISAAHEEAAEGGVDAVVLNFEGLEYMNSGGIGLLVTTLIRAQRSGHRLMAYGLTD
                     HYCQIFSLTRLDEAIEIFDDESAALAAV"
     gene            complement(791..1132)
                     /locus_tag="VFU96_12215"
     CDS             complement(791..1132)
                     /locus_tag="VFU96_12215"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU5114073.1"
                     /translation="MESVFEATVTRTDEGSVVVMSGTVNRAAKQGLETAYEEASAHPG
                     RILMDFRSVDYINSTGIAVIVGVLAMARAEDREVGAFGLTDHYREVFSITRLSDFMTI
                     YDDETAAVAAG"
     gene            complement(1132..2709)
                     /locus_tag="VFU96_12220"
     CDS             complement(1132..2709)
                     /locus_tag="VFU96_12220"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEU5114074.1"
                     /translation="MRRRRAEEETQVGDETQIPESKTRLIEAIPIPMGDPLETLLLSS
                     GGPVVLRDLELESDTVDTLREAGVELIVPLIGQGELLGALYLGPRLSDQPYSTDDRRL
                     LGSLASQVAPAMKVAQLVREQQVEAKERERIHQELQVAALIQQTLLPKELPAIAGWHV
                     DAFYRPARAVGGDFYDFIALEDGRLGVVIGDVTDKGVPAALVMATCRSMLRAAAQRHD
                     SPSAVLADVNDGLVPEIPPAMFVTCLYAIIDTEAGEVVFANAGHNLPYVRHDGGVLEL
                     RATGMPLGLMPGMGYDETKYEMSAGEVMVLTSDGITEAHNTEGEMYGFARLMDRVARR
                     PINDDVLNAVVTELEAFTGEDAEQEDDITLVVVKRTASAQASAETFEQRRQVMAKFGI
                     PSEEGNERIAIAKVAAAVEELGLEPAQLERLKTAVGETVMNAIEHGNENRSELDVDVQ
                     VCANEESVSVLVSDQGGDRLIPEATVPDLEAKLAGEQTPRGWGLFLIEKMVDEVRSTS
                     DGETHTIELVMNRKGAS"
     gene            complement(2732..>3154)
                     /locus_tag="VFU96_12225"
     CDS             complement(2732..>3154)
                     /locus_tag="VFU96_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5114075.1"
                     /translation="IPISILFAVLRYRLYDIDRILSRTVSYVIVIGLLAGVYLLGLTA
                     MTNFLASDSSLSVAASTLAVAALFNPLRRRVQARVERRFNRSRYDAQHVMDQFSGSLR
                     EDVDQDTVVEGWVGVVSETMHPTSISVWVRNDFGTMER"
BASE COUNT          592 a         1028 c          949 g          585 t
ORIGIN      
        1 cgagacccca accccaactc gccacccagt cggcgaggag ttctgacaca cccggcagtc
       61 cggcgaggcc gtcggcgaag agcacggcca ggccgatgag gagcaacagc cacccggtcg
      121 gatgaccgtt gcgccgaacg acgatgaggc ttccgatggc ggcgtagatc agaaatccga
      181 cggcgccgag gatgtcgtcc ccgagggcac gatctcccag agtcagctga acggtggcga
      241 gcccaagagg gatcaggagg aggacgccga gcaacgccca gcgacctgcc cgcgcccacc
      301 cctcgattgg ctctgtcgtc tgcgccaccc gctcattttg acagcgagag cgttacggaa
      361 gcgctacaga atcctgatcc gtcgcggatc catcacgagc tatcgactcg tgccgagtcg
      421 agggggtcag accgcggcca gcgctgccga ctcgtcgtcg aagatctcga tggcctcgtc
      481 gagacgggtc agcgagaaga tctggcagta gtgatcggtc aggccgtagg ccatgagacg
      541 gtggccgctg cgctgagccc ggatcagggt ggtgaccagg agcccgatgc caccgctgtt
      601 catgtactcg agaccctcga aattgagtac cacagcatcg acgcctccct cggcggcctc
      661 ctcatgagcg gcgctgatct ctttctcgga gaacccagtg atctcccctt cgatgtcgat
      721 gacccgtacc cggggagctg cctcccgcac gttcatggtc acattggctg tcggcatgat
      781 gctcctctcc tcacccggcc gcgactgcag ccgtctcatc gtcgtaaatg gtcatgaaat
      841 cggacagacg ggtgatgctg aacacctccc ggtagtggtc cgtcaaaccg aatgccccaa
      901 cctcacggtc ctcagcccga gccatcgcca atacgccgac gatgaccgcg attcccgtgg
      961 aattgatgta atccaccgat ctgaagtcca tcaggatccg cccggggtgg gcagacgcct
     1021 cctcgtaggc cgtctccagg ccctgcttgg ctgcccggtt cacggtcccg ctcatgacca
     1081 cgacagaccc ttcgtcagtc ctggtcaccg ttgcctcgaa cacgctttcc atcaggaggc
     1141 tcctttccgg ttcatcacga gctcaatcgt gtgggtttca ccgtcactgg tcgagcgcac
     1201 ctcgtcgacc atcttctcga tcaagaaaag gccccatccg cgaggggttt gctctccggc
     1261 gagcttggcc tcgaggtcgg ggacggtggc ctcggggatc aaacggtcgc ctccctgatc
     1321 gctgacgagg acactgaccg actcctcgtt ggcacaaacc tgaacgtcta cgtcgagctc
     1381 ggagcgattc tcgttgccgt gctcgatggc attcatcacg gtctcgccca ctgcggtctt
     1441 caaccgctcc agctgtgccg gctcgagacc gagctcctcg accgcggccg caaccttggc
     1501 gatggcgatg cgctcgttgc cctcttcgct ggggatgcca aatttggcca tcacctggcg
     1561 gcgctgctcg aacgtctctg cagaggcctg agccgaagct gttctcttca ccacgaccag
     1621 cgttatgtcg tcttcctgct cggcgtcctc accggtgaag gcctccagtt cggtgaccac
     1681 ggcgtttagg acgtcgtcgt tgatcggcct tcgggcgacg cggtccatga gccgggcaaa
     1741 gccatacatc tcaccttcgg tgttgtgggc ctctgttatc ccgtcactgg tcagaaccat
     1801 cacttcaccg gcggacattt cgtactttgt ctcgtcgtag cccatgcccg gcatgagccc
     1861 gaggggcatg ccggtggctc tcaactcgag gacaccgcca tcgtgtctca catagggcag
     1921 gttgtggccg gcgttggcga acacgacctc tccggcctcc gtatcgatga tggcgtagag
     1981 acaggtcacg aacatggcgg gcgggatctc cggcaccagg ccatcgttga catcggccag
     2041 gaccgcggag ggagagtcgt gacgctgggc tgccgcgcgc aacatcgacc tacaggtggc
     2101 catcaccagt gcggcaggca cgcccttgtc cgtgacgtcg ccgatgacaa cgccaagccg
     2161 gccatcctcg agcgcgatga agtcatagaa gtcgccgccg acagcacggg ccggccgata
     2221 gaaggcatcg acgtgccagc cggcgatcgc gggcaattcc ttggggagga gggtctgttg
     2281 gatcagggcc gcgacctgga gctcctggtg gatgcgttcc cgctccttcg cctcgacctg
     2341 ctgctcgcgg acgagttggg ccaccttcat ggccggcgcc acctgagacg cgagactgcc
     2401 gagaagcctg cgatcgtcgg tcgaataggg ctggtcggat agccgggggc ccaggtatag
     2461 ggcaccgagg agctctccct ggccaatcag cggcacgatg agctccaccc cggcctcccg
     2521 gagcgtgtcc acggtgtccg actccagctc gaggtcgcgc aaaacgactg gtccgcctga
     2581 ggaaaggaga agggtctcca agggatctcc catcgggatc gggatcgcct cgatcagccg
     2641 ggtcttcgac tccgggatct gggtttcgtc tccgacctgt gtctcttctt cggctctgcg
     2701 ccgtctcatc agctgaacca tggccccatt tctatctctc catcgtcccg aaatcgttac
     2761 gaacccatac cgaaatcgat gtcgggtgca tcgtctcgga caccactccc acccagccct
     2821 cgacgaccgt gtcctggtcg acgtcctcgc gcaatgagcc ggagaattga tccatcacgt
     2881 gttgcgcgtc atagcgcgat cggttgaaac gtctctccac ccgtgcctga acccgtcttc
     2941 gcaagggatt gaagagggcg gcaacggcaa gagtagaagc cgccacggac aacgaggagt
     3001 cagaggcgag gaaattggtc atcgcggtga gtccgagcag atacaccccg gcaagaaggc
     3061 cgatcacgat gacatatgac acggtccgtg acaggatccg atcgatgtcg taaaggcgat
     3121 atcgcagaac ggcaaacagg atcgagatcg ggat
//
LOCUS       DASTXO010000575         2200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_551507, whole genome shotgun sequence.
ACCESSION   DASTXO010000575 DASTXO010000000
VERSION     DASTXO010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551507"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            311..1444
                     /locus_tag="VFU96_12230"
     CDS             311..1444
                     /locus_tag="VFU96_12230"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEU5114076.1"
                     /translation="MKLVVTAFVTLDGVIEAPGFDEHRDGRNAWALRVQRDDDEVFNR
                     NQAFTADAYLLGRKTYQIWAAFWPTATGDEELAERVNGIPKYVVSNTLTRADWSNSRI
                     ISGDVVGQVRDLKEQPGGDLVVYGSADLVDLLVEHDLVDEYRLLIYPTILGTGKHLFR
                     DGIDTHHLQLVGTQTFSSGVVLLTYQPQREEPTSRYVADFAWTQEQVRSLQAAQDVDR
                     VLASVMFTDLVDSTGRAAELGDRAWRRLIDRHDEVARSEVDRWMGRFVKSTGDGVLAT
                     FDAPTRALRCAFDLRDALARLGLEMRAAIHTGEIEVRDADVGGIAVHIAARALGSASP
                     HQVVVTRTVLDLATGTDLGFAPLGSVTLRGVPGQWELFAASLT"
     gene            1511..1906
                     /locus_tag="VFU96_12235"
     CDS             1511..1906
                     /locus_tag="VFU96_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5114077.1"
                     /translation="MTQPDLLQLLIKHTDAWNRHDLDALMDLFDEACVFEASGGDEVC
                     GKRYEGHTAVKAAFAGVLGSMSDAEWGNGRHYILGLDYGVSEWTLTGTLPDGRRLEVN
                     GCDFLTVRNGKILVKNSYRKQRPPIESRR"
BASE COUNT          404 a          688 c          717 g          391 t
ORIGIN      
        1 cggcgtccag tttgtcagac atgctgcgga atttgaccag ttggaacggt ctgcctcgaa
       61 aaccggggcg ggtttggcga aagaagaccg atggtccttg gagcacccgg accaggagag
      121 cgatgaccag tagaaccggc acccaaatag gtgcggtcac tagggtcaaa accacatcga
      181 agacgcgctt ggcccccaat ccagaaggtc tgcttcccac gttcacccag ggtgatacta
      241 aaccgccctt ccttgaccca ctagagcgag gtaggcgtca ctgcggcgcc gccgggcaga
      301 atgggtcgcc gtgaagctgg tggtcactgc gttcgtcacc ctggatggcg tgatcgaggc
      361 gcccggattc gatgagcacc gcgatggcag aaacgcctgg gcgctacggg tgcagaggga
      421 cgacgacgag gtcttcaacc gaaaccaggc gttcaccgct gacgcctacc tcctggggcg
      481 gaagacatat cagatctggg cggctttctg gccaaccgcc accggtgacg aggagctcgc
      541 cgagcgagtc aacgggatcc ccaagtacgt ggtttccaat acgttgacga gggccgactg
      601 gagcaattcg aggatcatca gcggcgatgt cgtcggtcag gtgagagatc tgaaggagca
      661 gccaggtgga gacctggtcg tgtacggcag tgccgacctc gttgacctgc tcgtggagca
      721 cgatctcgtc gatgaatatc ggctcctgat ctaccccacg atcttgggaa ccgggaaaca
      781 cctctttcgg gacgggatcg acacccacca tctgcagctc gtcggcaccc agaccttcag
      841 ctccggagtc gtcctcctga cctaccagcc ccagcgcgag gagccgacca gccgatacgt
      901 cgcagacttt gcctggaccc aggaacaggt tcgctccctg caggccgcgc aagacgtgga
      961 tcgggtgctg gcgtcggtga tgttcaccga cctggtcgac tccaccgggc gggcagccga
     1021 gctcggcgac cgcgcctggc ggcggctgat cgaccgtcac gacgaggtgg cacgctccga
     1081 ggtcgaccga tggatgggcc ggttcgtcaa gagcaccggc gatggagtgc tggccacctt
     1141 cgatgcgccg accagggcct tgcgctgcgc cttcgatctc cgcgacgccc tcgcccggct
     1201 gggactggag atgagggccg cgatccacac cggtgagatc gaagtgagag atgccgatgt
     1261 cggcggcatt gccgttcata tcgcggcccg cgccctggga tcggcctcgc cgcatcaggt
     1321 ggtggtgacc cggaccgtcc tcgacctcgc caccggtacc gacctcggct tcgctccgct
     1381 tggttcggtg accttgcgtg gggttcccgg acagtgggag ctattcgcgg cctcgctcac
     1441 ctaactggtg ccttcggcgg gatccggcat cgggtctgcg atcatcagcg cctccgaccg
     1501 aggaggtggg gtgacacagc cggacctgtt gcagcttctc atcaaacaca ccgatgcctg
     1561 gaaccgacac gacctcgatg ctctgatgga tctcttcgat gaggcctgtg tgttcgaagc
     1621 gtcgggtggg gacgaggtat gcggaaagcg gtacgagggc cacaccgcgg tgaaggcagc
     1681 tttcgccggg gtcctcgggt cgatgtccga cgccgagtgg ggcaacgggc gccactacat
     1741 cctcgggctc gattacgggg tgtccgagtg gaccctcacc ggcaccttgc ccgacgggcg
     1801 ccggctcgag gtcaacggtt gtgacttcct gaccgtgcgt aacgggaaga tcctcgtcaa
     1861 gaattcgtat cgcaaacagc gaccgccaat cgagagccgt cgataggcgg ttatccacga
     1921 tctggcttcg gcgacatcct cgaggtcgaa cacccggacc tcaccaggca tgatgaggcc
     1981 gaagacacga gcgagaccgg ggtaggcatc gttgacccgg agcggcgcat ccgctcgggc
     2041 cgtccctcgt cgtcacgccg atgctacgtt tcgcctgtgt gggtggcctc cgttctgatg
     2101 cgacgcgcgg agtcgacatg acgactctgc acggagcgtt ctgatgcgct cacacaggat
     2161 cgccgcagcc gcaattctca ctgctgccgt cgcttgtggc
//
LOCUS       DASTXO010000576         2467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_146200, whole genome shotgun sequence.
ACCESSION   DASTXO010000576 DASTXO010000000
VERSION     DASTXO010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146200"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VFU96_12240"
     CDS             complement(<1..376)
                     /locus_tag="VFU96_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019852721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit D"
                     /protein_id="HEU5114078.1"
                     /translation="MSDTEKTLDVWVAGTEEPDLAPTDEGAQEISIDESGRRLDEQFG
                     RVVEDDYVFDDDADEDERMILNMGPQHPSTHGVLRLQIELEGEIIRRTKPIIGYLHTG
                     MEKTGEQLNFSQGGTNVTRMYYL"
     gene            complement(376..777)
                     /locus_tag="VFU96_12245"
     CDS             complement(376..777)
                     /locus_tag="VFU96_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEU5114079.1"
                     /translation="MARVPNDRWLEFATAAKDTGFEVCVDVTAIDWMRQRPERFEVVA
                     NVLSMQHRLRLRLITTAPRQEPTVASLVPVWPGANFPEREAFDMFGIVFEGHPDLTRI
                     LMPDEWEGHPLRKDFGVGLVPVQFKESHKAI"
     gene            complement(826..1349)
                     /locus_tag="VFU96_12250"
                     /pseudo
     CDS             complement(826..1349)
                     /locus_tag="VFU96_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006133126.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
     gene            complement(1379..1789)
                     /gene="ndhC"
                     /locus_tag="VFU96_12255"
     CDS             complement(1379..1789)
                     /gene="ndhC"
                     /locus_tag="VFU96_12255"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEU5114080.1"
                     /translation="MTLADYLPIGIMFVLAAVFVAVSLLASSLVRPNNPTEAKLDPYE
                     SGIVPEMEPVQRFPVKFYLVAMLFVIFDVEIIFLFAWASIYDSLGWYGVAAIFIFTFF
                     VAETLAYVWKRGALDWNVHRRARYIPTDATDEAA"
     gene            complement(1833..2414)
                     /locus_tag="VFU96_12260"
     CDS             complement(1833..2414)
                     /locus_tag="VFU96_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007731101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEU5114081.1"
                     /translation="MAKLIVVEQLSLDGVMQAPGDPDEDRSGGFEHGGWAMPYFDEVA
                     GEEAGKSMAETNAFLFGRRTYEIMAAYWPQQPDDDMFAKVLNSLPKYVASTTLPEPLA
                     WSGSTLLKGDVAAAVTELKEEQEGNIVVLGSGQLARTLIEHDLVDDYVLSIHPLMLGG
                     GKRLFDEDGANRPLRMVDSKTSTTGVILATYRP"
BASE COUNT          454 a          786 c          758 g          469 t
ORIGIN      
        1 acaggtaata catacgagtg acgttggtgc cgccctggga gaagttgagc tgctctccgg
       61 tcttctccat cccggtgtgg agatatccga tgatcggctt ggtgcgccgg atgatctcgc
      121 cttccaattc gatctggagc cggagcaccc cgtgggtgga gggatgctga gggcccatgt
      181 tgaggatcat cctctcgtcc tcgtcggcat cgtcgtcgaa cacgtagtcg tcctcgacca
      241 cccgcccgaa ctgctcgtcg agacgccgac ccgactcgtc gatcgagatc tcctgggcac
      301 cttcgtcggt cggggccagg tcaggctcct cggtgccggc gacccagaca tcgagggtct
      361 tttcggtgtc gctcatcaga tggccttgtg actttccttg aactggaccg gcactagtcc
      421 caccccgaag tcctttcgca gcggatggcc ttcccactcg tcgggcatca agatccgggt
      481 caggtcggga tgcccttcga acacgatccc gaacatgtcg aaggcctcac gctccgggaa
      541 gttggcaccg ggccacaccg gcaccagcga ggcgacggtc ggctcctgtc ggggagcggt
      601 cgtgatcagg cggagccgga ggcgatgctg catggagagc acgttggcta cgacctcgaa
      661 ccgttcagga cgctggcgca tccagtcgat cgcggtgacg tcgacacata cctcgaaccc
      721 ggtgtccttg gctgcggtgg cgaactcgag ccagcggtca tttggcaccc gcgccacgtc
      781 ctggcccagt gaatcctccc agtcgacgtc gtcgaaacgc tcgaacaacc ctctcaggat
      841 ctcgttctcc gggagtggcc gctcaagcat ctcgacggcg gcttcggtgt ccacggtgcc
      901 ggcctcgtcc gtcaactggc gatctcgccc ttcatgatct tgtcgtgcag tgtcaggatg
      961 ccgtgcatca ggctctgcgg gccgggtggg catcccggca catagatgtc gaccgggacg
     1021 acctggtcca ctccctggac gagggcgtag ttgttgaaca ttccgcccgt ggaagcgcag
     1081 gctcccatcg agatgaccca cttcgggtcg ggcatctggt cgtagacctg acggagcacc
     1141 ggggccatct tttgactgac cctcccggcc accaccatca ggtctgactg gcgaggtgat
     1201 gcccggaaca cctccatccc gaaccgaccc aggtcgtaat gggccgcgcc ggtcgccatc
     1261 atctcgatgg cacagcaggc cagaccgaac gaggcgggga acatcgatcg ggtgcgggcc
     1321 catcgcgccg ccttgtcgat cgtggtcatg agcaggttgc ccggtgcggt cgtcgtcact
     1381 acgcggcctc gtcggtggcg tcggtcggga tgtagcgagc gcgacggtgc acgttccagt
     1441 cgagggcgcc ccgcttccag acgtaggcca gggtctcggc gacgaagaag gtgaagatga
     1501 agatcgccgc caccccgtac cagccgaggc tgtcgtatat cgaagcccag gcaaacagga
     1561 agatgatctc gacgtcgaag atgacgaaga gcatcgccac cagatagaac ttgaccggga
     1621 acctctgcac cggctccatc tcggggacga tcccgctctc gtaggggtcg agctttgcct
     1681 cagtcggatt gttgggccgg accaggctgg aggcgaggag ggacacggcg acgaaaactg
     1741 cggccaggac gaacatgatc ccgatcggca gatagtcggc cagagtcacc atctgagctt
     1801 aggacgggcc cgggagtggg gttaaatcag ccctagggtc gataggtggc caggatcacg
     1861 ccagtcgtgg atgtcttcga atcgaccatt cggagcggcc gattggcgcc atcctcgtcg
     1921 aagagccgct tgcctccgcc caacatcagc gggtggatcg atagaacata atcgtcgacc
     1981 agatcgtgct cgatcaaggt ccgggccaac tgcccgctgc ccaggacgac gatgttccct
     2041 tcctgctcct ccttgagctc cgtcactgct gcagcgacgt cgcccttcaa caaggtggag
     2101 ccagaccacg ccaagggctc ggggagtgtg gtcgacgcca catacttggg caggctgttg
     2161 agcaccttgg cgaacatgtc atcgtcgggc tgttggggcc aataggcggc catgatctcg
     2221 taggtcctcc gcccgaacag gaatgcgttg gtctctgcca tcgacttgcc ggcttcctct
     2281 ccggctacct cgtcgaaata gggcatcgcc caaccaccgt gttcgaaacc cccactgcgg
     2341 tcctcatcgg ggtcacccgg ggcctgcatc actccgtcca gagacagttg ctcgaccacg
     2401 atcagtttcg ccatcggcaa ctctttcccg cttgaaaaat cccaaaacaa ccgtatggat
     2461 tctatcc
//
LOCUS       DASTXO010000577         2846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_514583, whole genome shotgun sequence.
ACCESSION   DASTXO010000577 DASTXO010000000
VERSION     DASTXO010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514583"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VFU96_12265"
     CDS             <1..593
                     /locus_tag="VFU96_12265"
                     /inference="COORDINATES: protein motif:HMM:NF015369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU5114082.1"
                     /translation="QFSVPIERVRDGSALLVTRWDDAPLGIEHGGPIRFVLPGHYAMR
                     SVKWLDAIEGVVEPFAGHFVMRYRYLGDDRFEEASPVAEIQLRSVIADPGQGESVPAG
                     AIVVRGSAWSGGTPVAEVGVSMDGGQTWQTAELEPGSGPLAAVAWRREVLIEPGSHVV
                     MARATDSAGNSQPMSPPWNKNGYANNMVHTVEFEAR"
     gene            complement(596..1570)
                     /locus_tag="VFU96_12270"
     CDS             complement(596..1570)
                     /locus_tag="VFU96_12270"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5114083.1"
                     /translation="MTGLRPPPKAETTSRVFLRFVVLVVLLIAVAAAIWTTVTLDRIE
                     LIEDVALEDVEHATVVTAGDTVLNVVRSGEGTVPLVLLHDVDVAGGVLWDGVAAELDP
                     RFSVMRVDLPGFGLSERIPQEGSRHTVASMAEEVADAIRQTFDQPAVVAGVGLGGEVA
                     AEIAVTQPDLLAGLVMVDVDFYKPREWDEFVERLPWFGTAATYALETGGPLADGRWAP
                     NCESGGWCPTPSQIAARDLAETIVDTSESIHFFRRTPAASQVPSKLGEITTPTFYLWS
                     QAGEVPRESVDRVQEAMPAATVEVVADAWKVHLDTPGVVAATVAGFLQ"
     gene            complement(1676..2650)
                     /locus_tag="VFU96_12275"
     CDS             complement(1676..2650)
                     /locus_tag="VFU96_12275"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014954704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU5114084.1"
                     /translation="MAELTYRNAVAYGIAQEMRRDPALVMLGEDIAGAGGVFKTTVGL
                     LEEFGPDRIRDTPISEQAILGAAMGAAMTGIPVIAEIMFSDFIAVCFDLLANEIAKSR
                     YMTGGQVTIPIVVRTANGGGSRFGAQHSQSVENWVMMIPGLKVVAPSTPLDVVGLLAA
                     SIRDPDPVVFLEHKSLMADKEDVPDGEILDQLGVARVVREGTDCTIAALALMVPRAME
                     AAGRLEEQGISAEVVDIRTLVPLDTQTILGSVSKTSRLFTVEENPRLCGWGAEIASIV
                     ADEAFWDLDGPVVRITTPHIPLPAAEALEDMAIPSVDRITEMVTKHRG"
     gene            complement(2638..>2846)
                     /locus_tag="VFU96_12280"
     CDS             complement(2638..>2846)
                     /locus_tag="VFU96_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEU5114085.1"
                     /translation="AWKERDPIPMYHERLLRLGVPEGDLSTIQQDVARRVDEATQFAK
                     DGALPGEEHLLTDVWADGGSAWRN"
BASE COUNT          503 a          957 c          908 g          478 t
ORIGIN      
        1 atcagttctc ggttcccatc gaacgggtcc gggacgggtc ggcactgctc gtaactcgct
       61 gggacgacgc gccgctggga atcgagcacg gcgggccgat ccggtttgtg ttgcccgggc
      121 actacgccat gcgatccgtg aagtggctgg acgccatcga aggtgtggtc gagcccttcg
      181 ccggccactt cgtcatgcga taccggtacc tcggcgatga ccgattcgag gaggcctcac
      241 ccgtggccga gatccagctc agatcggtga tcgccgaccc cggccagggg gagtcggtcc
      301 cggctggtgc catagtcgtc agaggctcgg cctggtccgg gggcacgccc gtcgccgagg
      361 tcggggtctc gatggatggc ggtcaaactt ggcagacagc agagctggag ccgggctccg
      421 ggccactggc agcggtcgcc tggcgccggg aggtcctcat cgagccgggc agccacgtgg
      481 tcatggcccg cgccacggat tcggccggaa acagccaacc gatgagccca ccctggaaca
      541 agaacggcta cgccaacaac atggtccaca ccgtggagtt cgaggctcgc tagggctact
      601 gcaagaaccc ggcgactgtc gcggcaacca ctccgggggt gtcgagatga accttccatg
      661 cgtcggccac cacctcgacc gtcgcggcgg gcatggcttc ctgcacccgg tcgaccgatt
      721 ctcggggaac ttctcccgcc tggctccaga gatagaaggt tggcgtggtg atctcaccca
      781 gcttcgacgg cacctgagag gcggctgggg tccgacggaa gaaatgaatc gactcggacg
      841 tgtcgacgat tgtctcggcg aggtcgcgcg ccgcgatctg actcggcgtc gggcaccatc
      901 caccgctctc acagttcggc gcccagcgcc cgtcggcaag agggccaccg gtctcgaggg
      961 catatgtcgc cgcggtgccg aaccagggca gcctctcgac gaattcatcc cactcccgtg
     1021 gcttgtagaa gtcgacatcg accatcacca gcccggcgag gagatcgggc tgggtcactg
     1081 cgatctcggc ggcgacctct cccccgaggc cgacaccggc gacgacggcc ggctgatcga
     1141 aggtctgccg aatcgcgtcc gccacttcct cggccatgga ggccacggtg tgtctgcttc
     1201 cctcctgggg aatcctctcg ctgagaccga atccggggag gtcgacccgc atcacggaga
     1261 accggggatc gagctctgcc gccactccgt cccagaggac tcctccagcc acatcgacat
     1321 cgtggagcag tacgagagga acggtccctt cccccgatct gaccacattg aggactgtgt
     1381 cgccggcggt gaccaccgtg gcgtgctcca catcttccag agcaacgtct tcgatcagct
     1441 cgatgcgatc gagggtgacc gtggtccaga tcgcggccgc cactgcgatc aggagcacga
     1501 cgagtaccac gaagcgaagg aagacccggc tcgtggtttc agccttgggc ggcggtcgaa
     1561 gaccggtcat gtagtaagtg tctcggccgt ctggggccga ggtttagccc agatcgaaat
     1621 catctgtcac cgacggcccc gccattcctc ccaccaccct aggaagaggg gaacctcaac
     1681 ccctgtgttt ggtgaccatc tcggtgattc ggtcgacgga gggaatcgcc atgtcctcca
     1741 aggcctcggc ggcgggaagg gggatgtgcg gtgtggtgat ccggaccaca ggcccgtcga
     1801 gatcccagaa cgcctcatcg gcaacgatgg aggcgatttc cgccccccaa ccacacagtc
     1861 tggggttctc ctcgacggtg aacaggcgac ttgtcttgct caccgacccg aggatggtct
     1921 gggtgtccaa cggcaccaat gtgcggatgt cgaccacctc ggcagaaatc ccctgctcct
     1981 cgagccggcc cgctgcctcc atggcgcggg gcaccatcaa tgcgagggcg gcgatggtgc
     2041 agtcggtgcc ctcccgaacc accctggcca cgcccagctg gtcgaggatc tcaccgtccg
     2101 ggacgtcctc cttgtcggcc atgagcgact tgtgctcgag gaacaccacc ggatcggggt
     2161 cacgtatcga ggccgcgaga aggccgacca cgtccagcgg agtcgaaggg gcaaccacct
     2221 tgagaccggg gatcatcatc acccagttct ccacgctctg ggagtgctgt gcaccgaacc
     2281 gggacccccc accgttggcg gtgcgaacca cgatcgggat cgtcacctgg ccgccggtca
     2341 tatacctgga cttggcgatc tcgttggcaa gcaggtcgaa gcagactgcg atgaagtcgg
     2401 agaacatgat ctcggcgatg accgggatgc cggtcatggc cgcgcccatg gccgcgccga
     2461 ggatcgcctg ctccgagatc ggcgtgtccc tgatccggtc cggaccgaac tcctcgagga
     2521 ggccaacggt cgtcttgaac accccacccg caccggcgat gtcctctccg agcatcacca
     2581 gagccgggtc gcgccgcatc tcctgggcga tgccgtaggc gacggcgttc cgataggtca
     2641 gttccgccac gccgaacccc cgtcggccca cacgtcggta aggaggtgct cctctccggg
     2701 cagggcgccg tccttggcga actgcgtcgc ctcatcgact cgcctcgcca catcctgctg
     2761 gatcgtgctc agatcacctt cagggacacc aaggcgaagc agcctctcgt gatacatcgg
     2821 gatcgggtct cgctccttcc aggcct
//
LOCUS       DASTXO010000578         3642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1435693, whole genome shotgun sequence.
ACCESSION   DASTXO010000578 DASTXO010000000
VERSION     DASTXO010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435693"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VFU96_12285"
     CDS             complement(<1..710)
                     /locus_tag="VFU96_12285"
                     /inference="COORDINATES: protein motif:HMM:NF014411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEU5114086.1"
                     /translation="MGELRPNSIAVVLGTRPEIIKLGHMIRLLGPAARVIHTGQHYDA
                     GLTDVFFDAFGIDTSSELIGVGGKTRGDQIGVALSALERAFVDDRPDAVVVQGDTNST
                     LAAALAANAREIPLVHVEAGLRSFDRRMPEEHNRVLTDHLADRCLAPTSTALENLISE
                     GIPEERIIVTGNTIVEAVNTLLPSTSSRVEILKRHGLAPARYVLSTFHRPENVDDPTT
                     LARIFEELARMPLPVLLPL"
     gene            836..>3642
                     /gene="uvrA"
                     /locus_tag="VFU96_12290"
     CDS             836..>3642
                     /gene="uvrA"
                     /locus_tag="VFU96_12290"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU5114087.1"
                     /translation="MSTDSILIRGAREHNLKNLTVELPRNQLIVFTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMDKPDVDFIDGLSPAISIDQKTASRNPRSTVGT
                     VTEVHDYLRLLYARIGIAHCPNDGTPVERQTPQQIVDRILELPEGTRFQVLAPIVRGR
                     KGEYEGLLADLAKQGFARARVDGEVRELTEEIKLDRYHQHTIEVVVDRLVRKEGMEQR
                     LTQSLETALTLTDGTADIEIVDGPTLTFSQHLSCPKCGLSFEDLQPRNFSFNSPYGAC
                     TVCSGIGTRYQVDPELVIADGGLSLEEGVIPMWSSNRMRYYHRLLTGLGEVFGFSMET
                     PWKDLPDEIQQKILYGTGQDKITIKYTNRFGRKRQWQATFEGAIPFLNRRHESAESDS
                     AREYYQEYMAEVPCEACGGGRLNPISLAVTIADRNIADVSDLALGRAKEFFEDLKLTD
                     RESTIAERVLKEVKARIQFLLDVGLDYLTLSRSAATLAGGEAQRIRLATQIGSGLVGV
                     LYILDEPSIGLHQRDNQRLIETLLRLRDLGNTLIVVEHDEDTIRVADHIVDIGPGAGE
                     HGGHIVAEGTLADITAEPDSITGDYIAGRRQIPVPFIRRQGNGEAITIRGATENNLQG
                     IDVVFPLGMFTAVTGVSGSGKSTLIEEILWKALANRVNRARVVPGRHKKIEGIEHIDK
                     VIDIDQSPIGRTPRSNPATYTKLFDQIRALFAQTPEARARGYKPGRFSFNVKGGRCEA
                     CAGDGTIKIEMHFLPDVYVPCEVCGGRRYNRETLEILWKGHDISDVLEMSVEEALGFF
                     ENQPKIVRILQTLYDVGLGYVRLGQPATTLSGGEAQRIKLSSELGRRSTGRTFYILDE
                     PTTGLHFEDIRKLLQVLQRLVDAGNTVVVIEHNLDVVKSVDHVIDLGPEGGSGGGRVL
                     AIGTPEEVAQVGESYTGKFLR"
BASE COUNT          734 a         1136 c         1142 g          630 t
ORIGIN      
        1 agaggtagca gtactggtag gggcattcga gcgagctctt cgaagatcct ggcaagcgtc
       61 gtgggatcgt ccacattctc gggtcggtgg aaagtcgaga ggacgtagcg tgctggcgcg
      121 aggccatggc gcttcaggat ctccacgcgg ctgcttgtcg aaggcagcaa cgtattcacc
      181 gcctcgacga tggtgttgcc ggtgacgatg attctttcct cggggattcc ctcggagatc
      241 aagttttcta atgccgtcga cgtaggtgcc aaacatcggt cagccaggtg atcggtgaga
      301 acccggttgt gctcttcagg catccgccgg tcgaagcttc gaaggccggc ctcgacatga
      361 accagcggta tctcgcgggc attggctgcc aaagcggccg ccagagtcga gttcgtgtct
      421 ccctgcacca ccaccgcgtc gggacgatca tcgacaaacg cgcgttccag ggcggatagc
      481 gccacaccga tctgatcgcc ccgagtcttg ccgcccaccc caatcagctc gctcgacgtg
      541 tcgatgccga aggcatcgaa gaagacgtcg gtgagtcccg cgtcataatg ctggccggtg
      601 tgaatgaccc gggccgcggg gccgagtagg cggatcatgt gcccgagctt gatgatctcg
      661 ggtcgtgtcc ccagcacgac agcgatcgaa ttcggcctga gctcacccat tgttcttaga
      721 tcgtctcgct gatcgacaag ctgaactgcg atcccgcaac gaacaggtgt tcgccgtaga
      781 tctggattac actgtcgtaa ctaaacggcg taaccaacgg ctaccagggc ggagcgtgtc
      841 caccgactcg attctcatca ggggcgcccg tgagcacaac ctgaagaacc tcacggtcga
      901 gctgccccga aaccaactca tcgtcttcac cggtctctcc gggtcgggca agtcatcgct
      961 ggccttcgac accatctacg ccgaggggca acgccggtat gtcgagtcac tgtccgccta
     1021 cgcccgtcag ttcctggggc agatggacaa gcccgacgtc gatttcatcg acgggctctc
     1081 acctgcgatc tcgatcgacc agaagactgc ctcgcgtaac ccacgttcga cggtagggac
     1141 ggtgaccgag gttcacgact acttgcgtct tctctacgcc cgcattggca tcgctcactg
     1201 cccgaacgac ggcacgccgg tggagcggca gactccgcag cagatcgtcg atcgcatcct
     1261 ggagcttccc gaaggcacca ggttccaggt gctggcgccc atcgtgagag gacggaaggg
     1321 ggagtacgag ggattactcg ccgacctggc caaacagggt tttgcccgag cccgggttga
     1381 cggcgaggtg agggagctga ccgaggagat caagctcgat cgctatcacc agcacaccat
     1441 cgaagtggta gtcgatcgcc tcgtccgcaa ggaagggatg gagcagaggc tcacccagtc
     1501 gttggagacc gccctcaccc tcaccgacgg caccgccgac atcgagatcg tcgacgggcc
     1561 caccctgacc ttctcccagc acctgtcctg cccgaaatgc gggctctcct tcgaggatct
     1621 gcagccccgt aacttctcat tcaacagccc atacggagcg tgcacggtgt gttccggaat
     1681 cggcaccagg tatcaagtcg atccggaatt ggtcatcgcg gacggcgggc tgtccctcga
     1741 agagggggtg atcccgatgt ggtcttcgaa ccgcatgcgg tactaccacc gactgctcac
     1801 gggccttggc gaggtgttcg gcttctccat ggagaccccg tggaaggacc tgcccgacga
     1861 gatccagcag aagatcctct acggcacggg acaggacaag atcacgatca aatacaccaa
     1921 ccgcttcggg cgcaagcggc agtggcaggc caccttcgaa ggggccatac ccttcctcaa
     1981 ccgtcgtcac gagtcggctg agagcgactc ggcccgggag tactaccagg agtacatggc
     2041 cgaggtcccc tgtgaagcgt gtggcggggg ccggctcaac ccgatctccc tcgcggtcac
     2101 catcgccgat cgcaacatcg ccgacgtatc cgatctcgct ctcggccggg ccaaggagtt
     2161 cttcgaggac ctgaagctca ccgaccggga gagcaccatc gccgagcgtg tcctaaaaga
     2221 ggtcaaggcg aggattcagt tcctcctcga cgtgggcctc gactacctga ccctcagccg
     2281 gtcggcagcc accctggccg gtggggaggc gcagcggatc cgactcgcca cgcagatcgg
     2341 ctcggggctg gttggggtgc tctacatcct cgacgagccg tcgatcgggc tgcatcagcg
     2401 ggacaaccag cgtctgatcg agaccctgct ccgtctccgt gacctgggta acaccctcat
     2461 cgtggtcgag cacgacgagg acacgatcag ggtggccgac cacatcgtgg acatcgggcc
     2521 aggagccggt gagcacggag gtcacatcgt ggccgaaggc accctcgcgg acatcaccgc
     2581 cgagcccgac tcgatcacag gggactacat cgccggacgc cgtcagatac ccgtcccgtt
     2641 catcagacgg cagggcaacg gagaggcaat caccatccgc ggcgcgaccg agaacaacct
     2701 gcaggggatc gacgttgtct tcccgctcgg gatgttcacc gcggtcaccg gggtgtcggg
     2761 gtcgggcaag tcgacgctga tcgaggagat cctctggaag gcactggcca atcgggtgaa
     2821 ccgagcaagg gtggtgccgg gaaggcacaa gaagatcgag gggatcgagc acatcgacaa
     2881 ggtcatagac atcgaccaat ccccgatagg acgcaccccg cggtcgaacc cggcgacgta
     2941 caccaagctc ttcgaccaga tccgggcgct cttcgcccag acaccggagg cgagggcacg
     3001 cgggtacaag ccggggcggt tctcgttcaa tgtgaagggt ggtcgatgcg aggcgtgcgc
     3061 cggcgacggc acgatcaaga tcgagatgca tttcctgccc gatgtctatg tgccgtgtga
     3121 ggtctgtggg ggtcggcgat acaacagaga gacgctggag atcctgtgga agggacacga
     3181 catatcggat gtgctcgaga tgtcggtcga ggaggcgctc gggttcttcg agaaccaacc
     3241 aaagatcgtc cgcatactgc aaaccctcta cgacgtcggg ctcggctacg tcagattggg
     3301 gcagccggcg accaccctct ccggtggtga ggcacagcgg atcaaactct cctccgagct
     3361 ggggcggcga tcgaccggtc gcactttcta catcctcgac gagccgacga ccgggctcca
     3421 tttcgaggac atcagaaagc tgctccaggt cctgcagcgt ctcgtcgacg ccggcaacac
     3481 cgtcgtcgtg atcgagcaca acctggacgt ggtcaagtcc gtcgaccacg tgatcgactt
     3541 gggccccgaa ggcggcagcg gcgggggacg ggtgctggcc atcggcactc ccgaggaggt
     3601 cgctcaagtg ggcgagagtt acaccggaaa atttctcagg ga
//
LOCUS       DASTXO010000579         2726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1251470, whole genome shotgun sequence.
ACCESSION   DASTXO010000579 DASTXO010000000
VERSION     DASTXO010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251470"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VFU96_12295"
     CDS             <1..909
                     /locus_tag="VFU96_12295"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU5114088.1"
                     /translation="GVMSRLGIDHESLKEHNPEIITSSVTGFGEYGPAKDKAAFDLVV
                     QAYSGHVDITGPADGDPARVGIPIADIAGGIYSCISVLAALVGRELHGKGEHADVAML
                     DTLVSLLSYDAVDHLNSGRKLTRLGTAHAHMVPWQAFQTSDGYVVVAARDEKFWQNLC
                     DAIGRPDLKDDPRTVDNTARVANRDQVVPILADVFSTKTNAEWLSILDEYDIPNAPVN
                     DMAQVFTDPQVLARDMVGTYKHPTLGDIRFPPTPIKWSEWERPNETAPMLGQHTIEVL
                     TTRLGLNQGEVRQMADEGIVTTWPEN"
     gene            919..1875
                     /locus_tag="VFU96_12300"
     CDS             919..1875
                     /locus_tag="VFU96_12300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEU5114089.1"
                     /translation="MRFGTFVPQGWKGDQDGIPAEAQWDRILDFARLIEDSGYDSIWV
                     YDHFHTHPVVKQESTFEAWTLMAALATATDRARLGQMCTCALYRPPSMLAKIASSIDV
                     ISRGRLDVGIGAGWSQKEFEAYGYRYPGDGERLDMLEEAVQVLLAMWTEDEARFAGKH
                     YTVDGAINRPKPVQKPHPPLWIAGGGEKRTLRIVAQYGDFANYGDDIDEFRHKSKVLA
                     DHCAAVGRDFGEIGRTCHRMSVIGRDDADLSRKLDVAARRRGCTPQQFADEHFVATVP
                     HAVQEMGEFVDEGCTDMLLYFYDMGEGDSLELFASEVIPQLR"
     gene            1872..>2726
                     /locus_tag="VFU96_12305"
     CDS             1872..>2726
                     /locus_tag="VFU96_12305"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU5114090.1"
                     /translation="MRDLGRLLDPKTIAIVGLSADAAKHGGRVLANLRKLGFPGAVRG
                     VNPGLPAVDGVTVVATLAELPEPPDLVVCAIPASETRRVVMTAHGTGGLVLFAGGFGE
                     TGSDGALLQADLAEQAQLAGTRVLGPNSGGIIRPSVGLAASFLTCLDRDAGEIRSGPV
                     GLVTQSGGTGSYIHNLAAARGGGLAVSVSTGNEVDIKLGEAISAVSALDEVHVVLALI
                     ETVRDGEVFIEAVRDSMARGKPVVACRIGTGDRGRALMTTHTGAMAVPEKVLESVLDS
                     LGVIVGETP"
BASE COUNT          497 a          847 c          909 g          473 t
ORIGIN      
        1 ggggtgatgt cccgtcttgg tatcgaccat gagtcgttga aggagcacaa cccggagatc
       61 atcacctcgt cggtgaccgg tttcggggag tatgggccgg ccaaggacaa ggctgccttc
      121 gacctggtgg tccaggccta tagcggccat gtcgacatca ccggtcccgc cgacggtgat
      181 ccggcccggg tcgggatccc gatcgccgac atcgccgggg gtatctactc gtgcatttcg
      241 gtgctcgccg ctctggtcgg ccgtgaactg cacggcaagg gggaacacgc cgacgtggcc
      301 atgctcgaca ccctggtctc gttgctctcc tatgacgcgg tcgaccatct caactcggga
      361 cggaagctga cccggttggg aacagctcac gcccacatgg tcccctggca ggcatttcag
      421 accagcgacg gatatgtggt ggtcgccgcc cgagacgaga agttctggca gaacctttgt
      481 gatgcgatcg gacggcccga tctcaaggac gacccccgca ccgtcgacaa cacggcccgg
      541 gtggccaacc gggaccaggt ggtgccaatc ctcgccgatg tgttctcgac caagaccaac
      601 gccgaatggc tatccatcct cgatgaatac gacattccga acgcaccggt caacgacatg
      661 gcccaggtct tcaccgatcc tcaagtgttg gcccgtgaca tggtcggaac ctacaaacac
      721 cccaccctcg gcgacatacg cttcccgccc acacccatca aatggagcga atgggagcga
      781 cccaacgaga ccgcacccat gctcggccaa cacacgatcg aagtactcac gacgagactc
      841 ggcctgaacc aaggcgaagt caggcaaatg gctgacgaag gcatcgtcac cacctggcca
      901 gagaactgag gtagggacat gcgtttcggg acgttcgtgc cacagggatg gaagggggat
      961 caggacggga taccggctga ggcgcaatgg gatcgcatcc tcgacttcgc ccgtttgatc
     1021 gaggactcgg gctacgactc gatctgggtc tatgaccact tccacaccca tccggtggtg
     1081 aagcaggaga gcacattcga ggcttggacc ctgatggccg ccctcgccac ggcaaccgac
     1141 cgcgctcgtc tggggcagat gtgcacctgc gccttgtatc gaccgccttc gatgttggcc
     1201 aagatcgctt cgtccatcga cgtgatctcc agggggcggc tcgacgtcgg gatcggagcc
     1261 ggatggtcac agaaggagtt cgaggcttac ggctatcgct atcccggcga cggggagcgt
     1321 ctcgacatgc tggaggaggc ggttcaggtg ctcctcgcga tgtggaccga ggacgaggcc
     1381 cggttcgccg gcaagcacta caccgtcgac ggcgcaatca accgtcccaa gccggtccag
     1441 aaacctcacc cgccactttg gatcgcgggg ggcggggaga agcggaccct gcgaatcgtc
     1501 gctcagtacg gggacttcgc caactatggc gacgacatcg acgaattccg gcacaagtcg
     1561 aaagtgctgg ccgatcactg tgctgcggtg ggtcgtgact tcggcgaaat cgggcgcacc
     1621 tgtcaccgga tgtctgtcat cggccgggac gatgcggatc tgtcacgcaa gctcgatgtg
     1681 gcggcgagac gtcgcggctg tactcctcag cagttcgccg acgagcattt cgtggccacg
     1741 gtgccccatg cagttcagga gatgggcgag ttcgtggacg agggctgcac cgacatgctc
     1801 ctctatttct acgatatggg ggagggcgac agtctcgagc tgttcgcctc cgaggtgatc
     1861 ccccagcttc gatgagagat ctgggtcggc tcctcgaccc gaagacgatt gccatcgtcg
     1921 gtctctccgc cgacgcggcc aagcacggag ggcgggtgct ggccaacctg cgcaagctgg
     1981 gcttcccggg tgcggtgcgg ggggtcaacc cgggtcttcc ggccgtggac ggggtgaccg
     2041 tggtcgccac cctcgccgag ctccccgagc cacccgacct ggtggtgtgc gccatcccgg
     2101 catcggagac gaggagggtg gtcatgaccg cccatggaac cggcggcctg gtcctcttcg
     2161 ccggcgggtt cggcgagacc gggtcggacg gcgcgctgct ccaggcggac ctcgcagaac
     2221 aggcgcagtt ggccggaacc agggtgctcg ggcccaacag cggagggatc atcagacctt
     2281 cggtcggcct ggccgcctcg tttctcacct gtctcgatcg agatgcgggg gagatccgct
     2341 ctgggccggt cgggttggtc acccagagcg gcggcaccgg cagctacatc cacaatctgg
     2401 ccgcggcccg cggcggaggg ctggcggtct cggtatccac cggcaacgag gtcgacatca
     2461 agttggggga ggcgatctcg gcggtgtcgg ccctggacga agtgcatgtg gtgctcgcgt
     2521 tgatcgagac cgtccgcgac ggggaggtct tcatcgaagc cgtccgtgat tcgatggctc
     2581 gtggcaagcc ggtggtggcc tgccggatcg gaaccggcga tcggggcagg gcgttgatga
     2641 ccactcacac cggggcgatg gcggtgccgg agaaagttct cgaaagcgtt ctggactctc
     2701 tcggtgtgat agtcggcgag acgccg
//
LOCUS       DASTXO010000580         2060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_514715, whole genome shotgun sequence.
ACCESSION   DASTXO010000580 DASTXO010000000
VERSION     DASTXO010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514715"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..542
                     /locus_tag="VFU96_12310"
     CDS             138..542
                     /locus_tag="VFU96_12310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03667 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEU5114091.1"
                     /translation="MLETRPELTAQLDEELVGWLTTVTPDGQPQSSVVWFLRDGDDLL
                     VYSRPGATKLSNIATNPKVAFNLRGDDVADAVATFEATAMIVDSPTPPHGNAAYLTKY
                     REEISRLGWTPEQFSNDYSALIRISLHRIRSW"
     gene            659..1090
                     /locus_tag="VFU96_12315"
     CDS             659..1090
                     /locus_tag="VFU96_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5114092.1"
                     /translation="MSTSATQPGTTTRAGRSGKGLGIAAAALAVVIAAGFVFAVNQGN
                     DATRSAVQSEPSGPAQAAAERHADRVAAQAQAESHPGFNIEKTKMELANQVAVISEYP
                     GLNPEKTRTEMLIDKLDEQIYVPSTEQATELNQPEWDNRIP"
     gene            complement(1514..1972)
                     /locus_tag="VFU96_12320"
     CDS             complement(1514..1972)
                     /locus_tag="VFU96_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5114093.1"
                     /translation="MSDSANQQEESLLSLLRRGLAQSDPSPADVTEFAKAALSWRTID
                     ADLAQLSYDSSEESNSASLRGVATSRMLAFEAGELTIDLEHEPLSGHLLGQLEPAGSM
                     TVELHVLDTVLVTESDELGRFSFDGIQSGPFALVIRLEGDEVVKTEWVIL"
BASE COUNT          402 a          660 c          630 g          368 t
ORIGIN      
        1 ggacatggtt gggagcgccc cagatccacg ccttggagct ctccagtggc aacacgttgt
       61 cgacggcaga tggtgtggaa accctcgcaa tgatcgggcc tgcgggagtc ctcgcctcca
      121 atcgctagct tgccccgatg ctcgagacgc gacctgagct cactgctcag ttggatgagg
      181 agttggtcgg ctggctgacc acggtcacgc ccgacggcca gccgcagagc tcggtggtct
      241 ggttcctccg agatggagat gacctgctcg tctacagcag gcccggtgcg acgaagctgt
      301 ccaacatcgc gaccaacccc aaggtcgcct tcaacctccg cggcgacgac gtggcagatg
      361 cggttgccac gttcgaggcg accgcgatga tcgtcgattc ccctacccca ccgcacggga
      421 acgcggcata tctgaccaaa taccgggagg agatcagccg tctcggatgg accccggagc
      481 agttctccaa tgactattcc gccctgatcc gcatcagcct ccaccgcatt cgttcctggt
      541 agcccaccac ttctctgtgg gcgacaggta ccggcgaagt cccgtgtatc agggccggct
      601 actgcgactc ctaccagcga cgggggcaga tcgcccccga ttcgggaagg gagagatcat
      661 gtcaacgagt gctacccagc cgggtaccac cacacgggca ggacgttcgg gtaagggtct
      721 cgggatcgcc gcggccgctc tcgccgtcgt aatcgccgcc ggcttcgttt tcgccgtcaa
      781 ccaggggaac gacgccacca ggtccgcagt tcagagtgag ccgtcgggtc cggcgcaggc
      841 cgctgccgaa cgtcacgccg atcgggtagc cgctcaggcc caggccgagt cgcatccggg
      901 gttcaacatc gagaagacaa agatggaact ggccaaccag gtcgccgtga tcagcgagta
      961 cccggggctc aacccggaga agaccaggac ggagatgctc atcgacaagc tcgacgagca
     1021 gatctacgtc ccgagcacgg agcaggcgac cgagctgaat cagcccgagt gggacaaccg
     1081 gatcccctga ccttcacgta cacagagaaa cagggccgcc ggcaggcggc cctttttcta
     1141 tggaaccttt gcgtaagaga agacccccac ctgagggcgg gggtcttcct actccgtttg
     1201 cgaggcctgt gcctcagcca ttctcagaag gcggggcgaa caatgaggcg tggcaggagg
     1261 tgagaccgat gcttcacctg aatccgattc tccttcgagc ggttgccgag ccacctctgg
     1321 acggggtggt cgagctgctc gatcgggcga atcgtccaag cccagcgccg ggtatttctc
     1381 ggttcctggg attcggacat gtatgtcatg tcgcgccttt cctcgctcac ggctcgtcgt
     1441 cgatcgacga cgattcacat atggagagga ggcgggccct tcgctgatac cgaaagtgag
     1501 acccgtcgaa gggtcagaga atgacccact cggttttgac cacttcgtcc ccttcgagac
     1561 gaatcacgag ggcgaagggg ccggattgga tcccgtcgaa ggagaaccgc ccgagctcat
     1621 cggattccgt gacaagcacg gtgtcgagca cgtgcagctc cacggtcatc gaccccgccg
     1681 gctcgagttg ccccaagaga tgacctgata gcggttcatg ctccaggtcg atcgtcaatt
     1741 ccccggcctc gaaggcgagc atccgggagg tggcgacacc gcgcaacgag gctgaattcg
     1801 attcctcgct cgagtcgtat gacagctggg ccaggtcggc gtcgatggtc cgccaggaca
     1861 gcgccgcctt cgcgaattcg gtcacatccg ccggcgacgg atccgattgg gccaagcctc
     1921 tacggagcag cgaaagcagg ctctcttctt gctggttggc cgaatcgctc atgtcgcatc
     1981 ttctcctgat tccaaagagt cgcctccagc accgctgata cgcgccgaaa gtgctccctt
     2041 cagtctttcc aggcacctcg
//
LOCUS       DASTXO010000581         2703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_514759, whole genome shotgun sequence.
ACCESSION   DASTXO010000581 DASTXO010000000
VERSION     DASTXO010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514759"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..1761
                     /locus_tag="VFU96_12325"
     CDS             196..1761
                     /locus_tag="VFU96_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEU5114094.1"
                     /translation="MDADTIHRRRWATLIVLSLSLVVIGLDNTILNVALPTLVRELGA
                     TASQLQWMVDAYILVFAGLLLTMGALGDRFGRKLALTIGLVVFGLSSVAAAFSDSANT
                     LIGARALMGVGGALIMPATLSIVTNVFEGRERGRAIAVWAGVAGLGIILGPVIGGWLL
                     EHYWWGSIFLINVFVVIVAVVAGAFLVPESKDPKATPLDPLGAVLSIAGLVALVYAII
                     EAPARGWTDPIVVGAFIVAAVVLSAFLWWEAQTEHPMLQLSFFDNPRFSAASIAITLV
                     FFAMFGTVFLNTQYLQFVLGYTPLEAGLRVMPVATMIVAAPLSARLTERFGSKVVVAS
                     GLTIVAVALSILATITIDTGYGRVAVALAILGAGMGTAMAPATDSIMGSLPLAKAGVG
                     SAMNDTTRQIGGALGVAILGSILASSYGAAMDPVVSQLPGPAADVASDSIGGAAAVAA
                     QIGEAGAGLIRAASEAFIDGMNSAVWVAVGVALLGAIVTWAYLPAQPVVMATSDLAEG
                     ATPLDPARVEEVS"
     gene            1758..2369
                     /locus_tag="VFU96_12330"
     CDS             1758..2369
                     /locus_tag="VFU96_12330"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU5114095.1"
                     /translation="MTTSTRVGRPRDATVSFAVAGGVISLLREVGLSGLTMDAVASRA
                     GVSKASIYRRWASKEELLVDVIANLASENPIPDTGHFRDDLVELLHRMEAFLSEIEAG
                     QIFPRMAAEVHAATELGRRYAERVILPRRAMLAGMLSGAQARGELRDDLHIDVAVDML
                     TGPVILRMLLGGLVPYDADWSPRLVDGLLQGWAGGASASSTRI"
     gene            complement(2324..>2703)
                     /locus_tag="VFU96_12335"
     CDS             complement(2324..>2703)
                     /locus_tag="VFU96_12335"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEU5114096.1"
                     /translation="GSLEEAVSAAERLAPEHLALHVEQPGDLLRNLTSYGSVFLGGQS
                     AEAFADYGVGPNHVLPTAGGARFQSGLSVMTFLKAPTWSRLDDPSALVEDTVLLARLE
                     GLEAHARAAQIRVDEAEAPPAQP"
BASE COUNT          424 a          866 c          899 g          514 t
ORIGIN      
        1 acccctgacc agagggctcg ggctcgaact tccagccgaa caagcccgaa tagaagaccc
       61 gacccttctc tacgtcttcg acgccgaact cgacgtaagc caattcgccc gacaatcact
      121 ctcctttcgc cgggaatggc attctatctt tcgatacgat acggtatagt ttcgtaatta
      181 gccgaggagc cgaacatgga cgctgacacc attcatcgcc gccgctgggc gaccttgatc
      241 gttctctcgc tgagcctggt ggtgattggc ctcgacaaca cgatcctcaa cgtcgccctc
      301 cccaccctgg tccgggagct cggggcgacc gcgagccagc tgcagtggat ggtcgacgcc
      361 tacatcctcg tgttcgccgg cctcctgctc accatgggtg ccctgggcga tcgcttcggc
      421 cggaagctgg cgctgaccat cggtctggtc gttttcgggt tgagctcggt ggcggcagct
      481 ttctccgatt cggccaacac cttgatcggg gcacgggccc tgatgggcgt cgggggagcg
      541 ctgataatgc cggcgaccct ctcgatcgtg accaacgtgt tcgaggggag ggagcgtggc
      601 cgagcgatcg cggtgtgggc cggtgtggcc ggcctcggga tcattctcgg cccggtgatc
      661 ggcggatggc tgctcgagca ctactggtgg ggctcgatct ttctgatcaa tgtcttcgtc
      721 gtcatagtgg ctgtcgtagc cggcgccttc ctcgtcccgg aatccaaaga cccaaaggcg
      781 acccccctcg atccgctggg cgcggtgctc tcaatcgccg ggctcgtggc cttggtctac
      841 gccatcatcg aagcgccggc cagaggctgg accgatccga tagtggttgg cgccttcatc
      901 gtggccgccg tggtgttgtc ggctttcctc tggtgggagg cgcagaccga gcaccccatg
      961 ctccagctga gcttcttcga caacccgagg ttcagcgcgg ccagcattgc cataaccctg
     1021 gtgttcttcg ccatgttcgg cacggtcttc ttgaacaccc aatacctgca gttcgtgctc
     1081 ggctatacgc cgctcgaggc ggggctacga gtgatgccgg ttgcgacgat gatcgtggcg
     1141 gcaccgttgt cggcacgtct cacggaacga ttcgggagca aggtcgtggt ggcttcgggt
     1201 ctcaccatcg ttgctgtggc attgtccatc ctggccacca tcaccatcga caccgggtac
     1261 gggcgtgtcg ccgtggcgct cgccatcctc ggcgcgggaa tgggcaccgc gatggcgccg
     1321 gcgacggatt cgatcatggg gtccctgcct ttggccaaag cgggcgtcgg ctcggcgatg
     1381 aacgacacga ccaggcagat cggaggcgcc ctcggagtcg cgatcctcgg cagcatcctg
     1441 gcgtcgtcct atggcgccgc catggatccc gtggtcagtc agctgccggg gccggcagcc
     1501 gacgtcgcat cggactcgat aggcggtgcg gcggcggtcg ctgctcagat cggggaggcg
     1561 ggcgcgggcc tgatccgggc ggcctccgag gctttcatcg acgggatgaa ctctgccgtg
     1621 tgggtggccg tcggagtggc tctcctcggc gcgatcgtga cgtgggccta cctgccggca
     1681 caaccggtgg tgatggccac ctcggatctg gcggagggcg cgactcccct cgaccccgcc
     1741 cgggtcgaag aagtttcgtg accaccagca cgcgggtcgg cagaccccga gatgccacgg
     1801 tctccttcgc ggtggccggg ggggtcatca gcctgctccg tgaagttggg ctctccgggc
     1861 tcaccatgga tgcggttgcg tcgagggccg gggtgagcaa ggcaagcatc taccggcgat
     1921 gggcgtccaa agaggagctg ctggtcgatg tgatcgccaa cctggcctcc gagaatccga
     1981 tacccgacac tggtcatttc agagatgatc tcgtcgagct gctgcacagg atggaagcct
     2041 tcctctccga aatcgaggcc ggccagatct ttccgcgaat ggcagccgag gtccatgccg
     2101 cgaccgagct cggacgtcgt tatgccgaac gcgtgattct gccgagacgc gccatgctgg
     2161 ctgggatgct ctccggggcg caggcccgcg gcgagctacg ggacgatctc cacatcgacg
     2221 tggcggtcga catgctcacc ggccccgtca tccttcgcat gctcctcggg ggtctcgtac
     2281 cctacgacgc cgactggtct ccaagattgg tcgacggtct gcttcagggt tgggcaggag
     2341 gagcctcggc ctcgtcgacc cggatctgag ccgccctggc atgggcctcc agcccctcca
     2401 atcgggcgag gaggacggtg tcttcgacca acgccgacgg atcgtcgagc cgtgaccagg
     2461 tgggagcctt gaggaatgtc atgacagaga gcccggattg gaacctcgcc ccgcccgcgg
     2521 tggggaggac gtggttgggg ccgaccccgt agtcggcgaa cgcctcggca ctctgcccac
     2581 cgaggaacac cgagccatag gacgtgagat tcctcagcag atcgccgggt tgctcgacgt
     2641 gcagggcgag atgctctggc gccaggcgtt cggcggcgga cacggcctct tccagtgagc
     2701 cga
//
LOCUS       DASTXO010000582         2493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1325385, whole genome shotgun sequence.
ACCESSION   DASTXO010000582 DASTXO010000000
VERSION     DASTXO010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325385"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1230
                     /locus_tag="VFU96_12340"
     CDS             <1..1230
                     /locus_tag="VFU96_12340"
                     /inference="COORDINATES: protein motif:HMM:NF023591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5114097.1"
                     /translation="PYKLLDTIRSRSQRFDFHPVPIEELAGHLSLIADREGYKTDPAA
                     LIAISRHAGGSVRDSLSLLEQVAALGAGAVDVAGVRRALGLADSEAFMRLVEAVADQD
                     AKAALELVAELAADGVDLRRFVAESVAFFRGAFLAHYAPNLAEISDEPADVYESWRKA
                     AQLVSAADVLRAVDLLGDALVRLREGREERLMTELALIKLTRPETSSDTEALLSRVER
                     IERRLGKPDPVPAGSVSGAVEPSRSAPPAPAAVDPAPAEAPAPVGSANGEAETAQEPA
                     ADASGLDISFEQLQKVWPGLFGSLRDVLGARRWAFFREAVPAAVEGNVIVLEVPHGFH
                     LQSLQEDDAVSAIVATRAGDLLGTPVRVRFGLREAVADVSGDQTIDMTQLEERPETST
                     DPTSLLASELGAQVVDE"
     gene            1250..1573
                     /locus_tag="VFU96_12345"
     CDS             1250..1573
                     /locus_tag="VFU96_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005248645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU5114098.1"
                     /translation="MNRQPDMRQIMAQAQKMQEQLAAAQKELAGRTFEGSAGGGMVVA
                     TVTGAQELVDITISSEVVDPDDVEMLQDLVLAAVRQALEAASNAASEELGGLTGDLGL
                     GGLLG"
     gene            1573..2166
                     /gene="recR"
                     /locus_tag="VFU96_12350"
     CDS             1573..2166
                     /gene="recR"
                     /locus_tag="VFU96_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEU5114099.1"
                     /translation="MFEGPVQRLIDEMARLPGIGQKTAQRLAFHLLAVEEADARRLAT
                     AINDMRDQVTLCERCFNVAAGEQCAICRDSRRDNSLVCVVERPQDIIVVERTQEYRGL
                     YHVLGGSLSPINGVGPGQLRIGELRERIERETIEELIVATNPTVEGDATAMYLARELK
                     PLGVKVTRLASGLPVGGDLDYADELTLGRALAGRMEL"
     gene            2163..>2493
                     /locus_tag="VFU96_12355"
     CDS             2163..>2493
                     /locus_tag="VFU96_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5114100.1"
                     /translation="MSARIPVDERQRTAADRFMRRWLRVGDVVDENAILGARRSTTAA
                     IVVSGIRCIITYLLIPVLAPVIGISDAASAPVTIALSVLAIVMGITGVRRFWMADHRA
                     RWAYTTFI"
BASE COUNT          404 a          803 c          850 g          436 t
ORIGIN      
        1 ccttacaagt tgctggacac gatccggtcc cggagccaga gattcgactt ccacccggtg
       61 ccgatcgagg agctggcggg ccatctcagc ctgatcgccg atcgggaggg ttacaagacc
      121 gaccccgcgg ccctcatcgc catctcgcgc catgccggag ggtcggtgcg cgactccctg
      181 tcactgttgg agcaggtagc cgctctcggg gcgggcgcgg tggatgtggc cggggtgaga
      241 cgagccttgg gtctggcaga ttcggaggcc ttcatgcgcc tcgtcgaggc agtcgccgat
      301 caggacgcca aggccgcgct cgagttggtt gccgagctag ctgcagacgg tgtggacctg
      361 agaaggtttg tcgccgagtc tgttgccttc tttcgcggcg cattcctcgc ccattacgcg
      421 cccaatctgg ccgagatctc cgacgagcct gccgatgtgt acgagtcgtg gcgcaaggca
      481 gcccagctcg tctccgccgc cgacgtgttg cgggcggtcg acctgctcgg agatgcactg
      541 gtcaggctgc gtgaagggcg cgaggagcgg ctgatgacgg agttggccct gatcaagctc
      601 acgcggccgg agacatccag cgacaccgag gccttgcttt cgagggtgga acggatcgag
      661 cggcgtcttg gcaagcctga tccggttccg gccggctcgg tctccggcgc tgtggagccg
      721 tcaaggtcgg cgccccccgc ccctgcggcg gtggatcccg caccggccga ggcacccgct
      781 ccggttggga gcgctaacgg cgaagcggaa acagcgcagg agccggcagc ggatgcctcg
      841 ggcctcgata tctccttcga gcagctccag aaggtttggc cgggtctgtt cgggtctttg
      901 cgcgacgtac tcggcgccag acgctgggcc ttcttcaggg aggctgtgcc tgcagccgtc
      961 gagggcaatg tcatcgtcct cgaagtgccc cacgggtttc atctccagtc actgcaagaa
     1021 gacgacgcgg tctcggcaat cgtggctacc cgggccgggg accttcttgg gaccccagtg
     1081 cgagtgaggt tcgggctgag ggaggcggtc gccgacgtaa gcggggatca gaccatcgac
     1141 atgactcagc tcgaggagcg gcctgagacg tcgaccgacc cgacatcgct cctggccagt
     1201 gagctcggcg cccaggtggt cgacgaatag cccactaccg ttggcggcaa tgaaccgcca
     1261 acccgacatg cgtcagatca tggcgcaggc acaaaagatg caggagcagc tcgccgcggc
     1321 ccagaaagaa ctggctggga gaaccttcga gggctcggcc ggtggtggga tggtggtagc
     1381 cactgtcacc ggagcccaag aattggtcga tatcaccatc tcgtcggagg tggtggatcc
     1441 agacgatgtc gagatgctcc aggacctcgt gctggccgcg gtccgccagg ccctcgaggc
     1501 cgcttccaac gcggcttccg aggagctggg cgggctcacc ggggatctcg gcctcggcgg
     1561 cctgctcggt tgatgttcga aggcccggtc cagcgtctca tcgacgagat ggcacgactg
     1621 cccggcatcg ggcagaagac ggcgcaacgt ctcgcctttc atctcctcgc agtcgaggag
     1681 gccgatgcca ggcggctggc taccgcgatc aacgacatga gagaccaggt gaccctctgc
     1741 gagcgttgtt tcaatgtcgc cgccggcgag caatgcgcga tctgccgcga ctctcgccgc
     1801 gacaactcgc tggtctgcgt cgtggaacgc ccccaggaca tcatcgtcgt cgagcggacc
     1861 caggagtacc gcggcctgta ccacgtgctc ggaggctcgc tgtctccgat caatggcgtg
     1921 ggcccgggtc agctccggat cggcgaattg cgggagcgca tcgaacgaga gacgattgaa
     1981 gagctgatcg tggccaccaa ccccacggtc gagggtgacg ccaccgccat gtatctcgcc
     2041 cgcgagttga agccgctcgg ggtcaaagtg actcggctcg cctccgggct accggtcggt
     2101 ggcgacctcg actacgccga cgagctcaca ctgggtcggg ccctggccgg ccgcatggag
     2161 ttgtgagcgc gcggattccc gttgacgaac ggcagcggac cgccgccgac cggttcatgc
     2221 gccgttggct gcgtgtcggg gacgtcgtcg acgagaacgc gatcctcggc gctcgacgtt
     2281 cgacgaccgc cgccatcgtg gtctccggga tccgttgcat catcacctat ctgctgatcc
     2341 cggtcctggc cccggtgata ggcatctcgg acgcagcgag cgcgccggtc acgatcgccc
     2401 tttccgtgct cgccatcgtg atgggtatca ccggtgtccg gcgtttctgg atggccgatc
     2461 accgtgcccg gtgggcctac accaccttca tcg
//
LOCUS       DASTXO010000583         4619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_957038, whole genome shotgun sequence.
ACCESSION   DASTXO010000583 DASTXO010000000
VERSION     DASTXO010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957038"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VFU96_12360"
     CDS             complement(<1..322)
                     /locus_tag="VFU96_12360"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEU5114101.1"
                     /translation="MGLASSLGYQPGDQNVIQRGIIALASTRLLSAISRRVLPSLDRL
                     ALRVSGGRSTVTSLTSGLPVLWLTTRGAKTGAERTVPLLGFPLEDDLAVLGTHFGHET
                     TPGWV"
     gene            360..677
                     /gene="cutA"
                     /locus_tag="VFU96_12365"
     CDS             360..677
                     /gene="cutA"
                     /locus_tag="VFU96_12365"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEU5114102.1"
                     /translation="MDGQCLVLITCGDSAEASEISHQLVESRLAAGVQIVPIESIYRW
                     DGEIVEDHEWLMLVKTRRDRFEEIESVVLEMHSYEVPPVLMVAMDAGSNPYLDWVAEN
                     TSG"
     gene            complement(690..1457)
                     /locus_tag="VFU96_12370"
     CDS             complement(690..1457)
                     /locus_tag="VFU96_12370"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5114103.1"
                     /translation="MNTFVLIPGAWLGAEAWAEVAEALRGSGAQVLPISLPGLGERTS
                     EATAETDMEGYVNDVIAQILDADLRDVTLVGHSYGGSVASAVADRVSARLASVIYVDS
                     GPLPSGSSYIDFLDSSQQDFVNELVQDRGEGWLVPMPEWEEFGTGFAASLDGLGVAER
                     RLMRAGAAPQPLKTWTQPVTRRDGAEVRCIPKVLISCSFPTAEVKELIAAGHPWFAEM
                     SGPEWTFEELPTGHWPMFSRPQDLARLLLEVTTAKTG"
     gene            1517..2182
                     /locus_tag="VFU96_12375"
     CDS             1517..2182
                     /locus_tag="VFU96_12375"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU5114104.1"
                     /translation="MQEQRQVGQKAGLTAESVLDAARSIADQEGLATLTMRRLADALG
                     VMPNSLYTYYPTKDALLDALLDSFLGEIETEQLESMGWRDALTKIMDDSRRLLLSHPR
                     LVEVFLTRPSAGPNAGRLGETTFRALRRAGLHGDEAVAAFRILLIFSLGYAAFQAPRV
                     GDEERARRGESAFRSLPPERFQEVRTLATPLARPPDDETFLAGISWILDGIARSSVIP
                     SSD"
     gene            complement(2201..2551)
                     /gene="ssrA"
                     /locus_tag="VFU96_12380"
     tmRNA           complement(2201..2551)
                     /gene="ssrA"
                     /locus_tag="VFU96_12380"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2639..3124)
                     /gene="smpB"
                     /locus_tag="VFU96_12385"
     CDS             complement(2639..3124)
                     /gene="smpB"
                     /locus_tag="VFU96_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEU5114105.1"
                     /translation="MARSAQTERVKVVASNRRARRNYTVVETLEAGLALLGSEVKSLR
                     EGRLDLKDSYVSIKGGEAFLVGAYVAPYEFARDGGHEPERERKLLLHRREIDRIAGQI
                     AEKGLTVVPLQVYFKDGKAKVELGLAKGKTSYDKRETLKERDADREVERAMARARKPR
                     A"
     gene            complement(3124..4050)
                     /locus_tag="VFU96_12390"
     CDS             complement(3124..4050)
                     /locus_tag="VFU96_12390"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEU5114106.1"
                     /translation="MSRFFFLLREALVNLKRNFLVVAGAVLAVFISLSLAFGALVVNE
                     ILRLNTLAWQEGVHVIAFLQDEGSNGVPPGAHQALLDEVESWEEVNGAFYVDKAQAWV
                     EYQEIFAGQDELLEIDPTILPASIRIELVAIELHQSVKFKLEQQQQVVRRVDTAAEQI
                     EQLQNLSSVLNVLGIGMAIVLGVSAVVLIANTIRLAIYARRDEVEIMKLVGASNWYIR
                     IPFLLEGLIEGVVGAALAVFTVWLGASRLAQASEGFALFRLDVSNQFFFRWGLLFIIF
                     GAAAGVIGSLLGLSRYLREADGTGEEIPTGAA"
     gene            complement(4047..>4619)
                     /locus_tag="VFU96_12395"
     CDS             complement(4047..>4619)
                     /locus_tag="VFU96_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5114107.1"
                     /translation="GKSTLIRLMLRDEGVTRGKVWVAGKDITTLPKWKIPYLRRSVGT
                     VFQDYKLLPNKTVAENVSFALEVLGRPRSVIGPQVDQVLDLVGLADMGGRYPKQLSGG
                     EQQRVSVARAFVNRPPIMLADEPTGNLDPKTSVGIMKLLDRINRTGTTIVMATHDFAI
                     VDAMQRRVVALERGVVTRDEHSGRYDQGQA"
BASE COUNT          857 a         1467 c         1431 g          864 t
ORIGIN      
        1 gcacccatcc cggtgtcgtc tcatgaccga aatgggttcc aaggactgcc agatcatcct
       61 cgagcgggaa tccgagcaga gggaccgtcc gctccgcccc ggttttcgcc ccgcgggtcg
      121 tcagccacaa cacgggcagc ccgctggtca gcgaggtcac ggtcgatctg ccgccgctga
      181 cccggagcgc gagccggtca agcgagggaa gaaccctgcg actgatcgcg gacagcaatc
      241 tcgtcgaggc gagggcgatg atcccgcgct gaatcacgtt ctgatcgccc ggctgatagc
      301 cgaggctgga agcgaggccc acagccccac ggtacgcatc tataccatcg agcctccgaa
      361 tggacggaca atgcctcgtc ttgatcacat gtggtgattc agcggaagcc agcgaaatct
      421 cccatcagct cgtcgaatcg aggctggcgg cgggagtcca gatcgtcccg atcgagagca
      481 tctacagatg ggatggcgag atcgtcgagg accacgaatg gctgatgctg gtcaagacac
      541 gccgtgatcg gttcgaggag atcgagtccg tcgtgctcga gatgcattcc tacgaggtgc
      601 cgccggtgct gatggttgcc atggatgcgg gtagcaaccc gtatctcgac tgggtggccg
      661 agaacacgag cggctagctt cagcggcctt cagccggtct tggccgtggt gacctcgagg
      721 agaagccgag cgaggtcttg gggacgggag aacatcggcc aatgaccggt cggcagctcc
      781 tcgaaggtcc actcgggacc actcatctcg gcgaaccagg ggtgaccggc ggcgatcagt
      841 tctttgacct cggcggtggg aaagctgcag ctgatcaaga ccttgggaat gcatctcact
      901 tcggccccgt cgcggcgggt gaccggttga gtccaggtct tcaagggctg gggtgccgcc
      961 ccggctcgca tcagtcgtcg ttcggccact ccgagcccgt cgaggctggc cgcgaaccct
     1021 gtcccaaact cctcccattc gggcatgggg acgagccagc cttctccgcg atcctggacg
     1081 agctcgttga cgaagtcctg ttgcgacgag tcgaggaagt cgatgtagga ggatccgctc
     1141 ggaaggggtc cagaatcgac gtagatcacg gaggcgagcc tcgccgagac acgatcggcc
     1201 acagccgagg cgacagaacc tccgtagctg tggcccacga gcgtcacgtc tcgaaggtcg
     1261 gcgtcgagga tctgagcgat gacatcgttg acgtaaccct ccatgtcggt ctcggccgtc
     1321 gcttcgcttg tcctctcccc cagaccgggc aggctgatcg gaagaacctg cgcacccgat
     1381 ccacgcaggg cttcggccac ctccgcccag gcctcggctc caagccacgc tcccgggatc
     1441 aggacaaatg tgttcatgac tggcctcctg gttgttgtac gttgtacaac taactgtacc
     1501 gcgtacaatt ggtcccatgc aagagcaacg acaggtgggg caaaaggctg ggctcactgc
     1561 agaatcggtt ctcgacgccg cccggtccat cgcagaccag gaagggctgg cgacgttgac
     1621 catgcgtcgt ctcgccgacg cgctcggtgt gatgccgaac tccctctaca cctactaccc
     1681 caccaaggac gctttgctcg atgccctcct cgactcattt ctcggcgaga tagagaccga
     1741 gcagctcgag tcgatgggat ggcgggacgc cctcaccaag atcatggacg actcgcgtcg
     1801 cctgctgttg tcccatcccc gattggttga ggtcttcctc actcggccga gcgccgggcc
     1861 gaatgcgggt cgcctcggtg agacgacatt ccgggcactg cgacgagcag gactacacgg
     1921 cgatgaggcg gttgcggcgt ttcgcatcct gctgatcttc tccctcggat acgccgcctt
     1981 tcaggcgccg cgagtagggg atgaagaacg tgctcggcgc ggagagtccg cgtttcgcag
     2041 tcttccgccc gagagatttc aggaagtcag gacgctcgca acacctctgg cgaggccacc
     2101 ggatgacgag acatttcttg ccgggatcag ttggatcctc gatgggatcg cccgttcatc
     2161 tgttatccca tcgagcgact gacctgccgt ggcgggccac tggtggaggt gcggggagtc
     2221 gaaccccggt ccgtcgagcc gtcagcgccg gcatctccga gcgcagtcgt cagcgagctt
     2281 tcagcaacag gaactctgac gacgagtttc cctgcgccta tccggagttg gcttgacccc
     2341 acggtcccga aaccccgtgg ggagcatccc gctttgcggc gcccggcccc gaccgtgcgg
     2401 gattcggtcg ggcggacggg ctacttaatt aggcagccag tgcgaagttg tcttcggcac
     2461 ttattggttt ttccggtttg ttgacggggc cccggagacc ccggctcgct tccaacgcct
     2521 caacactcaa cgtcgaaacc gtttcacccc caatgggatg tgatcagact aacgcccctc
     2581 tggggctgac tcttccgctt tgtttgtcgc cgacaggcca tatgtggcct cacccaactc
     2641 aggcacgcgg tttccgtgcg cgtgccatcg cccgctcgac ctcacggtcg gcgtcccgtt
     2701 ccttgagggt ctcgcgtttg tcgtaggagg tcttgccctt ggccaggccc agttcgactt
     2761 tggccttgcc gtccttgaag tagacctgaa gcggcacaac ggttaggcct ttctcggcaa
     2821 tctgaccggc gatccggtcg atctcgcggc gatggagcag caacttccgc tcgcgttctg
     2881 gttcgtggcc gccgtcccgt gcgaactcgt agggcgcgac atatgcgcca accagaaatg
     2941 cctcgccacc cttgatcgag acgtaggagt ccttcagatc gagccgaccc tcccggagcg
     3001 acttgacctc ggatccgagc agagcgagcc ccgcctcgag ggtctcgaca acggtgtagt
     3061 tgcgacgagc cctccgattg ctggccacga ccttgacccg ctcggtctga gccgagcgcg
     3121 ccactaggcg gccccggtgg ggatctcttc cccggtcccg tcagcctcac ggagataacg
     3181 gctcaacccg aggagcgagc caatgacgcc cgccgcagcc ccgaagatga tgaagagcaa
     3241 tccccatcgg aagaagaact gattactcac gtcgagacgg aacaatgcaa agccttcact
     3301 ggcctgggcc agtcttgacg cgcccaacca gacggtgaag accgcgaggg cggcgccaac
     3361 caccccctcg atcaggcctt ccaagaggaa cgggatcctg atgtaccagt tcgatgcccc
     3421 gaccagcttc atgatctcga cctcgtcgcg ccgggcgtag atcgcaagac ggatggtgtt
     3481 ggcgatcagg actacggcgg acacgccgag gacgatggcc atgccgatcc cgagcacgtt
     3541 cagcacactg gaaaggttct gcagctgctc gatctgctcg gctgctgtgt cgacacgacg
     3601 caccacttgc tgttgttgtt cgagcttgaa cttgaccgac tggtgcaatt cgatggcgac
     3661 cagctctatt cggatcgacg caggcaggat cgtggggtcg atctcgagca gttcatcctg
     3721 cccggcgaag atctcctgat attcgaccca cgcctgcgcc ttgtcgacgt agaaagcgcc
     3781 attgacctcc tcccaggact cgacctcgtc gaggagagcc tgatgggcgc cgggcgggac
     3841 cccattggat ccttcgtcct ggaggaaggc gatgacatgt actccctcct gccaggcaag
     3901 ggtgttgaga cggaggatct cgttcaccac cagagcaccg aaggcaaggc tgagggagat
     3961 gaacacggcg aggaccgcac ccgccacgac gaggaaattt cgcttgaggt tgaccaacgc
     4021 ctcccggagg aggaagaaga accggctcat gcctggccct ggtcgtatcg gcccgagtgc
     4081 tcatctctgg tgacgacgcc tcgctccagc gcgaccaccc ttcgctgcat ggcgtcgacg
     4141 atggcgaagt cgtgcgtggc catgacgatg gtggtgccgg tgcgattgat ccggtcgagg
     4201 agcttcatga tcccgaccga ggtcttggga tcgaggttcc ctgtcggctc gtcggccaac
     4261 attatcggtg gccgattcac gaaggcgcgg gccactgaga cgcgctgctg ctcaccgccg
     4321 gagagctgtt tcgggtatcg accgcccata tcggcgagac cgaccaggtc gagcacctgg
     4381 tcgacctggg gcccgatcac cgagcggggc cgtcccagca cctccagggc aaaggagacg
     4441 ttttcagcca ccgtcttgtt tggcaggagc ttgtagtcct ggaacacggt ccccaccgat
     4501 cgtctcagat aggggatctt ccatttgggc agcgtggtga tgtccttgcc agccacccag
     4561 accttgcccc gggtgacacc ttcatccctg agcatcaaac ggatgagggt ggacttgcc
//
LOCUS       DASTXO010000584         3243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_219945, whole genome shotgun sequence.
ACCESSION   DASTXO010000584 DASTXO010000000
VERSION     DASTXO010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219945"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VFU96_12400"
     CDS             complement(<1..527)
                     /locus_tag="VFU96_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase subunit D"
                     /protein_id="HEU5114108.1"
                     /translation="MTTETEPRIRLDGGVVGESPLRPDGIPKLRGEFEYAQDLSAEGM
                     LWAATARSSHARARIVSIDLAPALAIGGVHACLTVDDVPGRATFGLEHPDQPVLARDE
                     VRFWGEPLAVVAAEDPDTARRAAAAIEVSYEPLPALTDPEEADRRDEVFRRLSIRRGD
                     QALRGSVVVDGEYEVG"
     gene            complement(524..988)
                     /locus_tag="VFU96_12405"
     CDS             complement(524..988)
                     /locus_tag="VFU96_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006143313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU5114109.1"
                     /translation="MIIELTVNGEPVTARVTNPGSLLDLLRDQIGLPGSKNACEQGEC
                     GSCSVILDGELVCSCLVMAADAAGSSVVTVEGVSLGDGLHPIQEAMHRAGAVQCGFCT
                     PGIVVAAVDLFSRNPRPSNTDIKEALAGNICRCTGYGAIIRALETVDMGAKG"
     gene            complement(985..1779)
                     /locus_tag="VFU96_12410"
     CDS             complement(985..1779)
                     /locus_tag="VFU96_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEU5114110.1"
                     /translation="MEIVHPNSIDEALAELGDGAIPLAGGTDLMVEVNFGHLRPERVI
                     GLRRVAEMHEWDGPRIGAGVTWRRIETHGPKALAEAARTVGSPQIRNAGTIGGNLGTA
                     SPAGDGHPFLAAVDASIELTSAESARRVGWSEFFTGVKETIRRPDELITAIHLPDELP
                     ERQCFGKIGIRNAMVIATVCAVVTRADDGKTTVALGSVAPTPVRASEAEAMISSMPNP
                     TSRDLSEFQRLVSAEVRPITDHRSTEEYRRHGAGVLARRLLERCLA"
     gene            complement(1838..3193)
                     /locus_tag="VFU96_12415"
     CDS             complement(1838..3193)
                     /locus_tag="VFU96_12415"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HEU5114111.1"
                     /translation="MGTLLVRHAEVLVTMDGEEIRDGGLLARDGWIEKVGPTAELPAS
                     ADEVVDLTDHVVLPGLVNTHHHLYQTLTRVVPGAQDSGLFDWLLTLYPIWAGMTPDHV
                     RVSTRLGLLELALSGCTTAFDHHYLWPNGSSVDDQIEGAAPVGVRFHVSRGSMSLGRS
                     DGGLPPDSVVETNDVILDDSRRVVDLFHDPSPGAMTRVVLAPCSPFSVTPDLMTATAE
                     LARELRVGMHTHLAETADEEDFCLGRFDRRPVEHMEDLGWAGPDVWYAHAVHVADDEV
                     VRMGHSGTGVAHCPTSNMRLASGLAPVSRYLEAGLRVGLGVDGSASNDSSHMMGEARQ
                     ALLLNRLGVSPGIGTGPQMTARQALRLATVGGASVLGRDDIGRLAPGLCADFIAYDLN
                     RVEFAGARHDPVAAVVMCAPVSVDHSWVGALRVVEDGQPVGVDMGGLIREHNRLAASL
                     V"
BASE COUNT          573 a         1109 c         1037 g          524 t
ORIGIN      
        1 cccacctcgt actcaccatc taccacgacg gagccgcgca gagcctggtc tccccttcgg
       61 atgctgagcc ggcgaaacac ctcatcacgg cggtcggcct cttccgggtc ggtcaaggcg
      121 gggagcggct cgtagctgac ttcgatcgcg gccgctgccc tcctcgccgt gtcggggtcc
      181 tctgccgcca ccaccgccag cggctctccc caaaacctca cctcatcgcg ggcgagaacc
      241 ggctggtcgg gatgctccag cccgaaggtg gcccttcccg ggacgtcgtc gaccgtgaga
      301 caggcgtgga ctccaccgat ggcgagcgcc ggagccaggt ctatcgagac gatgcgggcg
      361 cgggcgtgcg agctgcgggc tgtggccgcc cagagcatcc cctcggcgct caggtcctgg
      421 gcgtactcga actcgcctcg gagcttcggg attccgtcgg ggcgcagcgg cgattcaccc
      481 accacccctc cgtcgagccg gatccgcggc tccgtctccg tggtcacccc ttcgctccca
      541 tgtcgacggt ctccaaggcg cggatgatcg ccccgtagcc ggtgcatcgg cagatgttcc
      601 cggccagggc ttccttgata tcggtgttgc tcgggcgtgg gttccgggag aacaggtcta
      661 cagcggcgac cacgatgccc ggtgtgcaga agccgcattg caccgccccg gcgcggtgca
      721 tcgcttcctg gatcggatga agaccatcgc cgaggctgac tccctcgacc gtgaccaccg
      781 atgaaccggc agcatcggcc gccatcacga gacaggagca caccaactcg ccatcgagga
      841 tcacgctgca ggaaccgcat tcaccctgct cgcaggcatt cttcgacccg ggtagcccga
      901 tttggtctcg caggaggtcg agaaggctgc ccgggttggt gacacgggcc gtgaccggct
      961 ccccgttgac ggtgagctcg atgatcacgc caggcacctc tcgagcagac gtctggcaag
     1021 cacccccgcc ccgtgacgcc tgtattcctc ggttgaccga tgatcggtga tcggccgcac
     1081 ctccgcagag acgaggcgtt ggaactcgga gaggtccctc gaggtcgggt tgggcatcga
     1141 cgagatcatc gcctcggcct cggaggcccg gaccggggtg ggggccaccg aaccgagggc
     1201 aaccgtggtc ttgccatcgt cggcacgggt caccaccgcg cagaccgtgg cgatgaccat
     1261 cgcgttgcgg atcccgatct tgccgaaaca ttgcctctcg ggaagctcgt cagggagatg
     1321 gatcgccgtg atcaactcgt cagggcgtcg gattgtctcc ttcacgccgg tgaagaactc
     1381 gctccagccc actcttcggg cggattcagc agaggtcagc tcgatcgagg cgtcgaccgc
     1441 ggccaggaaa ggatgaccgt cgccggccgg gctggcggtg ccgagatttc ctccgatcgt
     1501 gcccgcattt cggatctgag gagagccaac ggtgcgggcg gcctcggcga gcgctttggg
     1561 gccgtgggtc tcgatgcgac gccaggtgac gcccgctcca atccggggac cgtcccactc
     1621 gtgcatttcg gcgacgcgac gcaagccgat gactcgctct gggcgcaggt ggccgaaatt
     1681 gacctcgacc atgaggtcgg tgccgccggc cagtgggatc gctccgtcgc ccagctcggc
     1741 gagggcctca tcgatcgagt tcgggtgcac gatctccact ggcgggcaaa gctagcgcta
     1801 cctgttcggg ttttctctcg agaccgcatg aagacggtca cacgagtgac gcggccagcc
     1861 gattgtgctc ccggatcaat ccccccatgt ccacaccgac gggttgacca tcctcgacaa
     1921 cccgtagggc gccgacccaa gaatgatcta ccgagaccgg tgcgcacatc actaccgcag
     1981 ccacgggatc gtgacgggcg ccggcgaact cgacccggtt caagtcgtaa gcgatgaaat
     2041 cggcacacag cccgggtgcg agtcttccga tgtcgtctcg ccccagcaca cttgcgccac
     2101 caactgtggc gaggcgcagc gcttgacgcg ccgtcatctg gggaccggtg ccgatgcccg
     2161 gcgaaacccc aagacgattg agcagcagcg cctggcgagc ctcgcccatc atgtgcgacg
     2221 agtcgttcga cgccgacccg tccacaccca gcccaactct gagccccgcc tccaggtagc
     2281 gggacaccgg agccagcccg gaggcaagac gcatgttgga ggtcgggcaa tgagccactc
     2341 cagtccccga atggcccatc cgcacgactt cgtcgtcggc cacgtggacg gcgtgggcgt
     2401 accagacatc aggccctgcc cagcccagat cctccatgtg ctccaccggc cggcgatcga
     2461 acctgcccag gcaaaagtct tcctcgtcag ctgtctcggc gagatgggtg tgcatgccta
     2521 cccgaagctc ccgggccagc tcggcggtcg cggtcatcag atcgggtgtc acactgaacg
     2581 gcgaacatgg ggcgaggacc acccgcgtca tcgccccggg cgaaggatcg tggaacaggt
     2641 cgaccacgcg acgggaatcg tccaggatga cgtcgttggt ctcgacgacg gagtccgggg
     2701 gcaggccgcc atctgaccga cccaggctca tcgacccccg cgagacgtga aagcgcacgc
     2761 cgaccggggc agcgccttcg atctgatcgt cgacggagga cccgttgggc cagagatagt
     2821 ggtgatcgaa ggccgtcgtg caaccgctca aggccaattc gagcagaccg agacgggtag
     2881 agacgcgaac atgatccgga gtcatccccg cccaaatcgg atagagggtg agaagccaat
     2941 cgaagagacc gctgtcctgg gcgcccggga ccacccgcgt cagagtctga tagaggtgat
     3001 gatgagtgtt gaccaagccg gggaggacga cgtggtcggt cagatccacg acctcgtcgg
     3061 ccgatgccgg caattccgcc gtggggccga ccttctcgat ccacccatcg cgcgcgagca
     3121 gcccgccgtc cctgatctcc tcgccgtcca tggtcactag cacttcggca tggcggacga
     3181 ggagggttcc catcgccacc aaagctaact gctgtcttag agtcgcccgc catgactctg
     3241 cat
//
LOCUS       DASTXO010000585        10628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1362600, whole genome shotgun sequence.
ACCESSION   DASTXO010000585 DASTXO010000000
VERSION     DASTXO010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 10628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10628
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362600"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VFU96_12420"
     CDS             <1..865
                     /locus_tag="VFU96_12420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5114112.1"
                     /translation="LFLLSIVVGWVMSGRVLRPVKEITNVAREIQASDLSRRIALEGP
                     DDEITRLAATFDGMLERLDRAFSSQRRFLADTSHDLRTPLAVIRSNVEVVADDERATV
                     TDWQEVGGIVRRNAVKMSEMIDGLLATARLQTGKAQAVAIDLADIVAAKSSEYRNLLT
                     EKGIVLESSVSPALVEGVPVALDRALTNLLDNAIGVSGTGSRIRIGAGEVGEWAWMAV
                     ADQGPGLPEEPEGGRIGLGLSIVEQIAEGHEGSVVSSIGPDGSGTTMTIWLPKSDTAD
                     SPPDFTPFTGA"
     gene            1108..2139
                     /locus_tag="VFU96_12425"
     CDS             1108..2139
                     /locus_tag="VFU96_12425"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU5114113.1"
                     /translation="MSPWLMAFLAFLLVVGSSAATYLITTVDNDEVPAAQTGAPGDEQ
                     EAAAADELTPGEEPVADAAAVILPSVVQIQTGQGVGSGVIYDSDGLILTAAHVVEGAD
                     TVTVRLADGEQVEGTVLGGTSGADVAVVQVDGTDLPAAELALDEEPQVGQMAIAVGSP
                     WGLQGTVTSGIISAVDQAIPQGASARAVLQTDAAINPGNSGGPLVDRQGRVIGINVSI
                     FSLSGANDGVGFAVPIDIAHDIAERVVAGEEIQNAYLGVSLGVVESGQGGALIDEVTP
                     GTGADEAGLQVGDLVISIDGTPVQDGGDLAAQVQTHQPGDTVDVVVVRDGAEVTVPVT
                     MSERPADLG"
     regulatory      2179..2305
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2384..3373
                     /locus_tag="VFU96_12430"
     CDS             2384..3373
                     /locus_tag="VFU96_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5114114.1"
                     /translation="MRRITLILTLLALVACGAEGGSSSSTDEVATTNTEATDPPTTTA
                     LDPTTTAADAGFPLTVEAANGQVTIAQSPTRIVSLAPTATEMLFAIGAGGQVVAVDDF
                     SNYPEDAPISDLSGFEPNIEAIAAYEPDLVVVSDDINDVVASLTAIDIPVLHNPAAST
                     MDETYAQIDLLGQATGHPEGSSALIASMQEEIDRLVGAVPETPEAYTYYHELDGSFFT
                     VTSATFIGQLYGMAGLDNIADEAEGADTGYPQLSAEYIIEADPDLVFLADTKCCGESL
                     ETVAARPGWDVMTAVTGGGVVELDDDIASRWGPRVVEHLAIIVDEVQALAEGG"
     gene            3447..4439
                     /locus_tag="VFU96_12435"
     CDS             3447..4439
                     /locus_tag="VFU96_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU5114115.1"
                     /translation="MGVLAGCAVLGIAVGAVDLPVRSVVAELLDKLPGVDIESGLSER
                     QAAILWELRVPRVILAGMVGGMLAMAGAGFQGVFRNPLADPYLLGVAAGAGLGATLAI
                     AYLPDASGWLISPLPVAAFAGAVLGVAVTYALGRSATRTYAAATLILAGVAVAAFLTA
                     TQTFIQQENSETFREVFAWIMGALGTAGWGEVTLILPYVVVSALGLLLYRRLMDVMGV
                     GDEEADSVGIKAARVRLVVVAAATLGTAAAVAVSGLIGFVGIIVPHAIRLVLGSSYRI
                     ILPLSLGFGAAFLIIADLIARTVIAPAEIPIGVITAFFGAPFFLVVLTRARRLS"
     gene            4436..5221
                     /locus_tag="VFU96_12440"
     CDS             4436..5221
                     /locus_tag="VFU96_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007454538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5114116.1"
                     /translation="MSRIGLVDLAVAYNGRRVVDGISMQIEPRTWVALIGPNGAGKST
                     LLRAVASLVPYAGQIDIGGVPTAEMTRRRLSELVGFLPQRPVMPEGVTVADYIAIGRT
                     PYVPYWGTESDHDREIIVGVLERLELTTLSDRHIDTLSGGEAQRVVLARALTQQSEIL
                     LLDEPTSALDVGHQQQVLELIDELRSEQGLTVLSALHDLTLAGQYADRLIMMRDGVVV
                     SDGTAKSVLTEESIMMNYDARVRVVDMGTTGLIVTPLRPERGS"
     gene            5221..5769
                     /locus_tag="VFU96_12445"
     CDS             5221..5769
                     /locus_tag="VFU96_12445"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008820 - cobinamide phosphate
                     guanylyltransferase activity [Evidence IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEU5114117.1"
                     /translation="MESGSSLTLLLGGARSGKSKIGAALVDAWDGPTGVIATAEALDE
                     EMTVRIERHRSARPDDWLVVEEPLDLVGALNAFESGTAVLIDCLTLWVSNLMGEHLDE
                     VEIELRATAAAGVAATRGGLTVVVSNEVGSGIVPVNEMARQYRDLLGRVNAIWADMAG
                     QVLLTVAGGVIPVSRIGEVGDG"
     gene            5762..6751
                     /gene="cobT"
                     /locus_tag="VFU96_12450"
     CDS             5762..6751
                     /gene="cobT"
                     /locus_tag="VFU96_12450"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007425248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEU5114118.1"
                     /translation="MDEMIASWLGDLPEPDSRSAALVRGRAERVLRPAGALQRLDDVA
                     VWLAGWQRTALPAVESPHVVLFAGDHGVASAGVSAYPAEITRSMVEAFRQGVATANVM
                     AADVGASVAVVDVGVGRPTGDISSMPALSPGRFIECWDAGRRAVSEASSADLLVLGEM
                     GIGNTTAAAAVCATVFGGPADLWTGRGTGIDEATLERKTALVAAAARRVDGAGPFEAL
                     RQVGGSELVAVASAALEARMRSIPVILDGFVVTAACAPLEVARPGALDHTVAGHCSAE
                     SGHRLLLEKLSKPPLIDLGLRLGEGTGALAALPLVRLAAKCVIDVATFDEWVT"
     gene            6748..7515
                     /locus_tag="VFU96_12455"
     CDS             6748..7515
                     /locus_tag="VFU96_12455"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEU5114119.1"
                     /translation="MRGLRAAVRLLTRIPIGSAGPDQLDTSGSIGWLPIVGGMIGLVV
                     AGGYGLLAEVLPELAAATLAVTLGIVVTGALHEDGLADTIDAFGAGGGPEETLRILKD
                     PAHGTYGVIALVLSIVLRVLSLAALPVTIALLALPAVHALGRGGAVGLIGSMPPATGD
                     GLGAAHSRSGSSAGILGGLLSAIAIGVTLLGWWGAVFAALAGVATLMVGMLAMRRISG
                     FTGDILGATEQTIEIVLLLVAAALASSGLLVAPWWSS"
     gene            7512..8048
                     /locus_tag="VFU96_12460"
     CDS             7512..8048
                     /locus_tag="VFU96_12460"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU5114120.1"
                     /translation="MTLWLVRHGDTEWSEQRRLCGWSDPALSPAGVAQARLLRARLRH
                     RRFEGTWSSDLARARDFARISFGEARSDTRLREIDFGDLEGKTWDECDQDTQRALVRF
                     EGFTAPGGESVDTLQERVLGFLAGLAPGNHLIFTHGGVIRLLQARAGSGPPPRPGELV
                     VLEWPVSSEKPAATRSLI"
     gene            8045..8614
                     /gene="cobO"
                     /locus_tag="VFU96_12465"
     CDS             8045..8614
                     /gene="cobO"
                     /locus_tag="VFU96_12465"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEU5114121.1"
                     /translation="MTSPDLPPTEAPPTGHHRAPSLVLVNTGDGKGKSTAAFGTALRA
                     IARGWKVGVVQFLKSGEWKVGEETIGRNLGIDWWALGDGFTWDSADIDESEAIAREAW
                     LSAKTRIESDEYDLLILDEITYPINWGWIDIGDVLTVISHRPTRLNLILTGRDAPASL
                     VEVADTVTEMRKVKHAFDAGVMARRGIDY"
     gene            complement(8636..>10628)
                     /locus_tag="VFU96_12470"
     CDS             complement(8636..>10628)
                     /locus_tag="VFU96_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5114122.1"
                     /translation="IREEHLVQISPLSVPGSDIRSLDGIASFDSVELFVNRARLVNPN
                     FELGLANAQQVAEVCRRLDGIPLAIELAAARLKVLSVGQLVERLDQQFALLAGTARDL
                     PPHQQTLETTLDWSYDFLGGAERALFTRLSVFNGGFTLEAAESVCSGGLVAREHVMNL
                     LGRLVETSLVMAADTDPIRYRLLEPISHYARMRLDEGAELSVLRDRHAEFFLAMAEEG
                     DNEILNIEQNVWADRLERDRYNLRAALQWLHESGRAQDAMAMAGALRWFWVIKREVSD
                     GTEWLERTLSNREGVDPSIVARALNGAGLLASRRLAFDEADTALTEAMEIYRQLGDRR
                     GEARQAYQLMATAWLRDDIEEAKRLAPEAERLTRDVGDTWMLAWTLAVWATMARVQGD
                     LDEAREKMTESHRLFLDHSGVLDVGWSALRLGAVARDEGDYNEANDRYTEGRALLVVA
                     GDSLGVAHADAGLGAMAWLAGDHDYALELFKSVLEGFSIYEEPANNLFEMKTMIQSNP
                     TTAELQRVVQQNRDRATVVSEKAGAKAALGEYLYHMGKTAHRQGQFERAETALIESLI
                     LCDSAEDTRGVAIAIAGLAVGAHAQGRDVLAARLFGLADWVASKHRVAVWPPPEEHDY
                     AAQVAATQDSIGSQDFAHATVAGESLTVADAISLVRSDV"
BASE COUNT         1742 a         3336 c         3619 g         1931 t
ORIGIN      
        1 cctgttcttg ctgagcatcg tcgttggatg ggtgatgtcg ggccgggtcc tcagaccggt
       61 gaaggagatc accaatgtcg cacgtgagat ccaggcttcc gacctgagtc gccgcatcgc
      121 tctggagggg cccgatgacg agatcacccg cctcgccgcg accttcgacg ggatgctcga
      181 gaggttggac cgggcattct ccagccagcg gcggtttctt gccgacacca gccacgatct
      241 ccgtacccct ctcgccgtga tccggtccaa tgtcgaagtg gtcgccgacg acgagcgggc
      301 aacggtgacc gactggcagg aggttggggg catcgttcga cgcaatgcgg tgaagatgtc
      361 cgagatgatc gatgggcttc tggccacagc ccgtctccag accggaaaag cacaggccgt
      421 tgcaatcgat ctcgccgaca tcgtcgccgc caagtcctcc gagtacagga atctcctgac
      481 ggaaaagggg atagtcctgg agagctcggt gtctcccgct ctggtcgaag gtgtccctgt
      541 cgccctcgac cgggccctga ccaatctcct cgacaacgcc atcggggtgt caggcaccgg
      601 atcgaggatc aggatcgggg caggagaggt aggcgaatgg gcctggatgg cggtggccga
      661 ccagggaccg gggcttcccg aggagccgga gggcggtcgc attggcctcg ggctctccat
      721 cgtggagcag atcgcagagg gccatgaggg ctccgtggtc tcctcgatcg ggcccgatgg
      781 ttcggggacg accatgacga tttggctccc caaatccgac accgcagatt ccccgccaga
      841 cttcacgcca tttacgggag cttgatctct ccggtcataa gttcgtatgc aggaggtttc
      901 tcaaggatga caaacgagga catgacccaa cacccaacac cgtcgccagc cgagcaaggc
      961 cccgtcggcg aagagagcac cgtcccccac ggatcggtag caacgcaggc gccggtgact
     1021 caggagacac ctgaggcccc tcagcctgcc caggtaacac caccgcccgt ggcgccggcc
     1081 ccgcccgctc agaagagccc ggctcggatg tcaccgtggc tgatggcatt ccttgccttt
     1141 ctgctcgtgg ttggctcctc cgcggcgacg tatctgatca ccacagtcga caacgacgag
     1201 gtgcctgccg cccaaaccgg cgcgcccggc gacgagcagg aagcagcggc tgccgacgag
     1261 ctgactccgg gtgaagagcc ggtggccgac gccgccgcag tgatccttcc ctcggtggtt
     1321 cagatccaga ctggccaggg cgtcggatcc ggtgtcatct atgactccga cggcttgatc
     1381 ctcacggccg cccatgtcgt cgagggggca gacacggtca ccgtgaggct tgccgatggc
     1441 gagcaggtcg aggggactgt cctcggtggc acgtcaggtg cggatgtggc cgtagtccag
     1501 gtggacggca ccgatttgcc ggcagccgag ctcgcgctcg atgaagaacc gcaggtgggc
     1561 cagatggcca ttgcagttgg cagcccatgg ggcctccagg gcaccgtcac ttccggaatc
     1621 atctcggccg tggaccaggc gatcccgcaa ggtgcttcgg cccgtgcggt cctccaaacc
     1681 gacgccgcga tcaatccggg caactctggt ggtccgctcg tggaccgtca gggtcgggtg
     1741 atcggaatca atgtctcgat cttcagcctc tctggtgcca acgatggtgt cggcttcgcc
     1801 gtcccgatcg atatcgctca tgacatcgcc gagcgagtcg tggccggtga ggagatccag
     1861 aacgcctatc tgggcgtgtc cctcggtgtg gtggagtcgg gtcagggcgg cgccctgatc
     1921 gacgaggtca ccccgggcac cggtgccgat gaggccgggc tccaggtcgg cgacctggtc
     1981 atctccatcg acgggacgcc agttcaggat ggcggcgacc tcgccgccca ggtccaaacc
     2041 catcaaccag gcgacacggt cgacgtcgtg gtggttcgtg acggcgccga agtgactgtc
     2101 ccggtgacca tgagcgaacg acccgccgac ctcggctgac ccaaggctcc tcctggtggg
     2161 cccggctatg ctggtcgggc ccaccatcat tgggtgggtg cctaggagaa cagcgaagcc
     2221 ggttagaccc cggtgctgtc ccgcaactgt gaagccccct aagtggggac gagccaggtc
     2281 gcctgctctc cagggccacg acgaaagtct cggatgaaga cggaagcgtg ggctcaggga
     2341 agccaccggc ctcttcatcc tccgataacg gaggatgtgt catatgcgaa ggatcaccct
     2401 gatcctcacc ttgctggcgc tcgtcgcctg tggtgccgaa ggtgggagct cctcttccac
     2461 cgatgaggta gcgaccacta acacggaggc gactgatccg ccgacgacga cggcgttgga
     2521 cccgaccacg actgctgccg atgccggatt cccgctgacc gtcgaggcgg cgaacgggca
     2581 ggtgacgatc gctcagagcc cgacacgaat cgtctccctg gctcccaccg ccaccgagat
     2641 gctgttcgcg ataggtgccg gggggcaggt cgtcgccgtc gacgacttct ccaactatcc
     2701 cgaagatgct ccgatcagcg acctgtcggg gttcgagccg aacatcgagg cgatcgcagc
     2761 ctacgagcca gacttggtcg tggtctccga cgacatcaac gacgtggtcg cttcgctgac
     2821 ggcgatcgac atccctgtcc tgcacaaccc cgcggcgagc actatggacg agacctacgc
     2881 ccagatcgat ttgctgggtc aggccaccgg tcacccggag ggttcctccg cattgatcgc
     2941 ttccatgcaa gaggagatcg atcggctggt gggtgccgtt cccgagacgc ctgaggcgta
     3001 cacctattac cacgaattgg acgggtcgtt cttcacagtg acctcggcga ccttcattgg
     3061 tcagctctat ggaatggccg gtctcgacaa catcgccgac gaggccgagg gggcggacac
     3121 cggctatccg cagctgtccg ccgagtacat catcgaagcc gaccccgatc tcgtgttcct
     3181 cgccgacacc aagtgttgcg gcgagtcgct ggagaccgtg gcggctcggc ccggttggga
     3241 tgtgatgacg gcggtgaccg gcggtggcgt ggtcgaactg gacgacgaca tcgcctcacg
     3301 gtggggtccg cgcgtcgtgg agcacctggc gatcatcgtc gatgaggtcc aggcgttggc
     3361 ggagggagga tgatcggggg ttgaccacgg agccagatcg aagcccacgc atctcgaagg
     3421 tcggttgggg ctcggcactc ctcgttctgg gggtgctggc gggatgtgcc gtgctcggaa
     3481 tcgcggtcgg tgcggtcgat ctgccggtcc gctcggtagt ggccgagctc ctggacaagt
     3541 tgccgggggt cgacatcgaa tcggggctgt cggaacgtca ggcagcgatc ctctgggagt
     3601 tgagggtgcc acgtgtgatc ctcgccggga tggtgggggg gatgctggcc atggccggcg
     3661 ccggctttca gggtgtgttt cgcaacccgc tcgccgaccc gtatctcctg ggagttgctg
     3721 cgggtgcggg cctcggcgcc acactggcca tcgcctacct ccccgacgcc agcggttggc
     3781 tgatcagccc attgcccgtg gccgcttttg ccggcgccgt tctcggggtt gccgtcacct
     3841 acgccctggg acgatcggcg acccggacct atgccgccgc aacgctcatc ctggccgggg
     3901 ttgcggtggc cgccttcctg acggccaccc agaccttcat ccagcaagag aactcggaga
     3961 cgttccgtga ggtgtttgcc tggatcatgg gcgccctcgg aacggcgggc tggggagagg
     4021 tcacactgat cctgccttac gtggtcgtga gcgccctcgg ccttctcctc tatcggcgtc
     4081 tcatggatgt gatgggcgtg ggagatgagg aagccgacag cgtcgggatc aaggcggcga
     4141 gagttcgatt ggtcgtagtg gccgcggcca cgctcggcac cgcggcagcg gtcgcggtga
     4201 gcggactgat cgggttcgtc ggcatcatcg tcccccatgc gattcgactc gtcctgggtt
     4261 ccagctatcg gatcatcctc ccgctgtcgc ttggctttgg cgctgcgttt ctgatcattg
     4321 ccgacctgat cgcccgaacc gtgatcgccc cggctgagat accgatcggg gtgatcaccg
     4381 cctttttcgg ggcgcccttt ttcttggtgg tgctgacacg ggcgaggcgg ctctcatgag
     4441 ccgtatcggg ctcgtcgacc tcgcggttgc ctacaacggt cggagggtcg tcgatggcat
     4501 ctcgatgcag atcgaaccga gaacctgggt ggcgttgatc ggccccaatg gtgctgggaa
     4561 gtcgaccttg ctccgggcgg tggcaagcct cgtgccgtac gcgggccaga tcgacatcgg
     4621 tggcgtgcca accgccgaga tgacccggag acgactgtcc gagctcgtgg gcttccttcc
     4681 ccagcgcccg gtgatgccgg agggagtaac ggtggccgat tacatcgcga tcggacgaac
     4741 tccgtatgtc ccttactggg gcaccgagtc ggatcacgat cgcgagatca tcgtcggggt
     4801 cctggagcga ttggagctca cgaccttgtc ggatcgccac atagacacgt tgtcgggcgg
     4861 ggaggcgcag cgtgtcgtcc tcgcacgggc actgacgcaa caatccgaga tcctgctcct
     4921 cgacgagccc acgtccgcgc tcgacgtggg acatcagcaa caggtcctgg agctgatcga
     4981 tgagctcaga tccgagcagg gattgacggt cctgagcgcg cttcatgacc tgacactggc
     5041 cggccagtac gccgatcgac tgatcatgat gagggatggc gtggtggtca gcgacggcac
     5101 cgccaagagt gtgttgaccg aggagagcat catgatgaac tacgacgccc gggtgcgagt
     5161 ggtggacatg gggaccaccg gtctgatcgt gacaccgttg cgacccgaac gaggctcttg
     5221 atggagagtg gatcatcgct gacgctgctc ctcggcggcg cgaggagcgg gaagtcgaag
     5281 atcggcgccg ccctcgtcga tgcctgggat gggccgaccg gcgtgatcgc gaccgcagag
     5341 gcgctcgatg aggagatgac cgtgcgcatc gagcgacacc gcagcgcccg ccctgacgac
     5401 tggctggtgg tagaagagcc tctcgacctc gtaggcgcgc tcaacgcgtt cgaatccggc
     5461 accgcggtgc tgatcgattg cctcacgttg tgggtgtcca acctgatggg cgagcatctg
     5521 gatgaggtcg agatcgagct gagggccacg gccgccgcag gcgtcgccgc cacccgggga
     5581 ggattgacgg tggtggtcag caacgaggtc gggtcgggga tcgtccccgt gaacgagatg
     5641 gcccgccagt accgggatct ccttggcagg gtcaacgcga tctgggccga catggccggt
     5701 caggtgctgc tcaccgtggc cggaggggtg atcccggtgt cgaggatcgg cgaggttggc
     5761 gatggatgag atgattgcgt cgtggctggg ggatctcccc gaaccggatt cccggtcggc
     5821 cgcgttggtc cgtgggcgcg ccgagcgtgt gctacgtccg gcaggggctc ttcagcggct
     5881 ggacgatgtg gcggtctggc tggccggatg gcagcgaaca gctctgcccg cggtggaatc
     5941 accgcatgtc gtgctttttg ccggagacca tggcgtcgct tctgccggtg tgagcgccta
     6001 tcccgccgag atcacccggt ccatggtgga agcctttcgc cagggcgtgg ccacggcgaa
     6061 cgtcatggcc gccgatgtag gggcctcagt cgctgtggtg gatgttgggg ttggtcggcc
     6121 gacgggtgac atctcctcga tgcccgctct gagcccgggc cgtttcatcg agtgctggga
     6181 tgccgggagg cgggcggtgt cggaagcgag cagcgccgat ctgttggttc tcggggagat
     6241 gggaatcggg aacacgaccg ccgctgccgc tgtgtgcgcc accgtgttcg gaggcccagc
     6301 cgacttgtgg accggtcggg gcaccgggat cgacgaggca accctcgagc gtaagacggc
     6361 tctcgtcgcc gcagctgcga gacgtgtcga cggcgccggc ccattcgagg cgctgcgaca
     6421 ggtcggcggg tcggagttgg tcgcggtcgc gtccgcagcc ctcgaggcga ggatgcgttc
     6481 gatcccggtc atcctggacg gtttcgtcgt caccgcggcc tgcgcccccc tcgaggtggc
     6541 gcggccaggc gctctcgatc acacggttgc cggtcattgc tcggccgaat cgggccaccg
     6601 actcctcctc gagaagctgt ccaagccccc tctcatcgat ctcgggctgc gactgggcga
     6661 ggggactggg gcactggcgg cgttgcctct ggtgaggttg gcggccaaat gcgtcataga
     6721 cgtggccacc ttcgacgagt gggtgacatg agaggtctgc gcgccgctgt gaggctcctc
     6781 acccgcatcc cgatcggcag cgccggtcct gaccagctcg acacctcggg ctcgatcggc
     6841 tggcttccga tagtcggagg gatgattgga ttggtggtag ccgggggata tggcctcctg
     6901 gcggaggtgt taccggaact ggccgcggcc acgctggccg tgacgctcgg gatcgtcgtc
     6961 accggcgccc tgcatgagga tgggttggcc gacaccatcg atgcattcgg cgccggtggt
     7021 ggaccggagg agaccctgcg aatcctgaag gaccctgcgc atggcaccta cggggtgatc
     7081 gctttggtcc tcagcatcgt cctgcgggtc ctgtcgctgg ccgccctccc ggtgacgatt
     7141 gccctgttgg cattgcccgc cgtccatgcg ctgggccgag gaggagcagt gggtttgatt
     7201 ggttccatgc ctcccgcgac cggggatggg ctgggggctg ctcattctcg atccggctcg
     7261 tcggcgggga tactcggggg cctgctcagc gcgatcgcga tcggtgtgac gctgttggga
     7321 tggtggggcg ctgtatttgc cgccctcgcc ggggttgcca ccctgatggt cggcatgctg
     7381 gcgatgcgtc ggatttcggg attcaccggc gacatcctcg gggcgacgga gcagaccatt
     7441 gaaatcgtcc tgctgttggt tgcagcggct cttgcctcat ccggcttgct cgtcgcaccc
     7501 tggtggtcct catgaccctg tggctggttc gtcatggcga caccgaatgg agcgagcagc
     7561 ggcggctctg tggttggtca gaccccgccc tcagcccggc aggggttgca caggcgaggc
     7621 tccttcgcgc ccggcttcga caccgccggt tcgaagggac ctggtcttcg gatctggccc
     7681 gggcccgtga cttcgctcgc atctcattcg gcgaggcgcg gtccgacacg aggctgaggg
     7741 agatcgactt tggtgacttg gagggcaaga cgtgggacga atgcgatcaa gacacccaga
     7801 gggccctggt gaggtttgag ggattcacgg caccgggtgg cgagtcggtc gacacgctgc
     7861 aggagagggt gctcggattc ctggccggcc tggcaccggg caatcacctc atcttcactc
     7921 acggaggggt gatccggctg ctgcaagcgc gagccggatc gggccctccc ccgaggccag
     7981 gcgagttggt cgtgctcgag tggccggtct cgagcgagaa gccagccgcg acaaggagcc
     8041 tgatatgaca agccctgacc tcccccccac cgaggcaccg ccgaccggcc accaccgggc
     8101 gccttcgctc gttctggtca acacggggga tggcaaggga aagtcgacgg ccgctttcgg
     8161 taccgcgctg agggcgattg cgcggggctg gaaagtggga gtcgtccagt tcctcaagtc
     8221 gggggagtgg aaggtgggcg aggagacgat cggccgcaat ctggggatcg actggtgggc
     8281 cctgggcgat gggttcacct gggattcggc cgacatcgac gagtccgagg ccatcgcccg
     8341 tgaggcatgg ctcagcgcca agacccgtat cgaatccgac gagtacgacc ttctcatcct
     8401 ggacgagatc acttatccca tcaactgggg ctggatcgac atcggcgacg ttttgaccgt
     8461 catctcgcac cgcccaaccc gtctcaatct catcctcacc ggccgcgatg cccccgcttc
     8521 cctcgtcgaa gtggccgaca ccgtgaccga gatgaggaag gtgaagcacg ccttcgacgc
     8581 gggcgtcatg gcgagacggg ggatcgacta ctgatttcag cctcccgcgg agacctcaga
     8641 cgtcgctgcg aaccagggag atcgcatctg cgacggtgag tgattcccct gccaccgtgg
     8701 cgtgggcgaa gtcctgactg ccgatgctat cctgcgtggc cgccacctgg gcggcgtagt
     8761 cgtgctcctc cgggggaggc cagacggcga cacggtgctt gctcgccacc caatcggcca
     8821 gtccgaagag acgtgcggca agcacgtcgc gcccttgcgc gtgggcgccc accgcgaggc
     8881 cggcgatggc gatggccacg cctcgcgtgt cttcggcgga gtcgcacagg atcaagctct
     8941 caatcagggc ggtctcggct cgctcgaact gaccctgccg gtgagccgtc ttgcccatgt
     9001 gatagaggta ctcaccgagt gcggccttgg cgccggcctt ctcgctgaca acagtggctc
     9061 ggtcccgatt ctgctgcacg acgcgctgca gttcggcggt ggtcgggttc gattggatca
     9121 tcgtcttcat ctcgaacagg ttgttggcgg gctcctcgta gatgctgaat ccttcgagca
     9181 ccgacttgaa caactccaac gcgtaatcgt gatcgccggc cagccaggcc atcgccccta
     9241 gcccggcgtc ggcgtgagcc actcccagtg agtcgccggc gacgacgagg agcgcccgtc
     9301 cttcggtgta tcggtcattt gcctcgttgt agtctccctc atcacgggcc acggcgccga
     9361 gcctgagagc tgaccatccc acgtcgagaa ctcccgaatg gtccaggaaa agcctgtggc
     9421 tctcggtcat cttctcccgc gcctcgtcca ggtctccctg cacacgcgcc atagtggccc
     9481 agactgcaag ggtccaggcc agcatccagg tgtcgccgac gtcccgggtg aggcgctcgg
     9541 cctctggggc gagacgtttc gcctcctcga tgtcgtcccg aagccacgct gtcgccatca
     9601 gctggtacgc ctggcgcgcc tcaccgcgtc tgtcaccgag ctgccggtag atctccatcg
     9661 cttcagtgag agccgtgtct gcctcgtcga aggcaagacg gcggctggcg agcagaccgg
     9721 cgccattcag tgctcgagcg acgatcgagg gatcaacacc ttcgcgattg gagagagtgc
     9781 gctcgagcca ctcagtgcca tcgctcacct cgcgtttgat gacccagaac caacggaggg
     9841 ccccagccat ggccatcgca tcctgggccc ggccagactc atgcagccac tgcaaagccg
     9901 cgcgaaggtt gtagcggtcg cgctccagcc ggtcagccca cacgttctgc tcgatgttca
     9961 ggatctcgtt gtcaccttcc tcggccatcg ccaggaagaa ctcggcgtgt cggtcgcgaa
    10021 gcacggagag ctcggcgcct tcgtccagcc gcatccgggc gtagtggctg atcggctcga
    10081 ggaggcggta gcggatcgga tcggtgtccg ctgccatgac cagcgaagtc tcgaccagtc
    10141 ggccgaggag gttcatcacg tgctctcggg ccaccaggcc accagaacag acgctttcgg
    10201 cagcctccag cgtgaagccg ccgttgaaga ccgacagccg ggtgaacaag gcgcgctccg
    10261 cgcctccgag gaagtcatag ctccagtcga gagtggtttc gagcgtctgt tggtgtggag
    10321 gcaggtctcg agccgtgcct gccaggaggg cgaactgctg gtcgaggcgt tcgaccagct
    10381 ggcccaccga caacaccttc aatcgggctg cggccagctc gattgcgagc ggaatgccgt
    10441 ccagtcgccg acagacctcc gcaacttgct gggcgttcgc cagaccaagc tcgaagttcg
    10501 ggttgaccag ccgtgcccgg ttgacgaaca gctcgacaga gtcgaaggag gcgatcccat
    10561 ccaacgagcg gatatcggag ccggggacgg aaaggggaga gatctgaacc aggtgctctt
    10621 ccctgatc
//
LOCUS       DASTXO010000586         3279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1326004, whole genome shotgun sequence.
ACCESSION   DASTXO010000586 DASTXO010000000
VERSION     DASTXO010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326004"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2067)
                     /locus_tag="VFU96_12475"
     CDS             complement(<1..2067)
                     /locus_tag="VFU96_12475"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020020015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEU5114123.1"
                     /translation="MSKGLRITRRFTEAGKDPYDKIEWARRDSRITNPDGSTVFEMTD
                     AEIPAGWSQVASDIMVSKYFRKAGVPQFDEDGNQILDESGQPELGPEKSAKQVFSRLA
                     GTWRHWGEKEGYFADSDDATAFEDELKYMLARQMAAPNSPQWFNTGLNWAYGLTGPAQ
                     GFWYVDGATGDLESSPDSYSRPAPHACFILSVRDDLVNPGGIMDLWVREARIFKFGSG
                     AGSNFSQIRAENERLSGGGKSSGVMSFLKIGDRAAGAIKSGGTTRRAAKMVILDVDHP
                     DIERFIDWKKVEEDKARVLINHGGLPADFNGDAYATVSGQNSNNSVRVTNDFIQTVID
                     DGDWDLINRTDGSVRKTVKARDLWDRIAEAAWACADPGLQFDTTVNEWHTSPAGGRIR
                     ASNPCSEYMFLDDTACNLASINLVRFHDDETGVFDIEAYQHAVRLWTIVLEISVAMAH
                     FPSPQIAQGSYDYRTLGLGYANLGSLLMRQGIAYDSDEGRAISGALTAILTGYSYATS
                     AEIAGVVGPFPRFAENREAMLRVIRNHRRAAYGDTDYEGVSHKVMAIDPDLCPADFLA
                     AARTSWDLALEQGEEHGYRNAQSSVIAPTGTIGLLMDCDTTGVEPDFALVKFKKLAGG
                     GYFKIANQSVAPALRHLGYEESQIKDVVEYIVGTSSIDGAPHINRETLTEKGFDDEDL
                     VKLEKAL"
     gene            complement(2370..2735)
                     /locus_tag="VFU96_12480"
     CDS             complement(2370..2735)
                     /locus_tag="VFU96_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018760628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5114124.1"
                     /translation="MSEHHRALAVVKGIHTVIWAFVEGAMLYLLASGAAGRSDRRAGA
                     AAAIVGVESLVFLANGARCPLTGLAESLGAEDGSVTDIYLPGWLARSLPLLHVPLIVT
                     AGWLHWRNLRGGREISEAT"
     gene            complement(2798..>3279)
                     /locus_tag="VFU96_12485"
     CDS             complement(2798..>3279)
                     /locus_tag="VFU96_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5114125.1"
                     /translation="GNTWAWPRTDNATQRFASALGEGRTGEFLVDRANVFVNVFVRRA
                     MRRRAPSGREMAMWRGPFPDQESRLPVRVFPRELTGSAAFLASIEERLGEIADKPTLL
                     FWADQDPAFRGTEKRRWESILTNRTGYVLRHAGHYWQDDAGPEAALMIRYWWDQAGL"
BASE COUNT          560 a         1051 c         1085 g          583 t
ORIGIN      
        1 caatgccttc tccagcttga ccagatcctc atcgtcgaag cctttctcgg tgagcgtctc
       61 acggttgatg tggggcgccc cgtcgatcga gctggtgccg acgatgtact cgaccacgtc
      121 cttgatctgg ctctcctcgt agccgaggtg cctcagagcg ggggccaccg actggttggc
      181 gatcttgaag taaccgccgc cggccagctt cttgaacttg accagggcga agtcgggctc
      241 gactccggtg gtgtcacagt ccatcaacag gccgatggtc ccggtggggg cgatcacgct
      301 ggactgggcg ttgcggtagc cgtgctcctc gccctgctcc agggccaggt cccatgacgt
      361 gcgagcggcg gcgaggaagt cggccgggca caggtcggga tcgatcgcca tcaccttgtg
      421 actgacaccc tcgtagtcgg tgtcgccata ggcggcgcga cgatgattcc ggatcaccct
      481 cagcatcgcc tcccgattct cggcgaaacg cgggaaggga ccgaccacac cggcgatctc
      541 cgccgacgtg gcataggagt acccggtgag gatggcggtc aacgccccac tgatggcacg
      601 accctcgtcg gagtcgtagg cgatgccctg acgcatcagg agcgatccga ggttggcgta
      661 tccgaggccg agggtccggt agtcgtagga gccctgggcg atctgcggtg aggggaaatg
      721 ggccatggcc accgagatct cgagcacgat ggtccagagc cggaccgcgt gctgataggc
      781 ctcgatgtcg aacacaccgg tctcgtcgtc gtggaacctg accaggttga tcgaggccag
      841 gttgcaggcg gtgtcgtcga ggaacatgta ctccgagcac gggttggacg cccggatccg
      901 accaccggcg gggctggtgt gccactcgtt gaccgtggtg tcgaactgaa gcccggggtc
      961 ggcgcaggcc caggccgcct cggcgatccg gtcccagagg tcgcgagcct tgaccgtctt
     1021 gcggaccgag ccatcggtcc ggttgatcag gtcccagtcg ccgtcgtcga tgacggtctg
     1081 gatgaagtcg ttggtgaccc gaaccgagtt gttcgagttc tgcccggaga ccgtggcgta
     1141 ggcgtcgccg ttgaagtcgg cgggcaggcc accgtggttg atcagcaccc gagccttgtc
     1201 ctcttcgact ttcttccagt cgatgaaccg ctcgatgtcg gggtgatcga cgtcgaggat
     1261 gaccatcttg gcggcgcgtc tcgtcgtccc tcccgacttg atggcgccgg cggcgcggtc
     1321 tccgatcttg aggaaggaca tgacaccgga tgacttgccc ccgccggaga gccgttcgtt
     1381 ctccgcccgg atctgcgaga agttggaacc ggcgcccgag ccgaacttga agatgcgagc
     1441 ctctctgacc cagaggtcca tgatcccgcc cgggttgacc aggtcgtccc tgacgctgag
     1501 gatgaagcag gcatgtgggg cgggccggct gtaggagtcg ggggaggact cgaggtcacc
     1561 ggtggcaccg tcgacatacc agaaaccctg ggcaggcccg gtcaggccgt aggcccagtt
     1621 gagcccggtg ttgaaccact gcggggagtt gggggcggcc atctgcctcg ccagcatgta
     1681 cttgagctcg tcctcgaagg cggtggcgtc atcgctgtcg gcgaagtagc cctccttctc
     1741 gccccaatgc cgccaggtgc cggcgagccg gctgaacacc tgcttggccg acttctccgg
     1801 tcccagttcc ggctgcccgc tctcgtcgag gatctggttg ccgtcttcgt cgaactgggg
     1861 gacaccggcc ttgcggaagt acttggacac cattatgtcc gaggccacct ggctccaacc
     1921 agccgggatc tccgcatcgg tcatctcgaa cacggtcgag ccatccgggt tggtgatccg
     1981 cgagtcgcga cgtgcccatt cgatcttgtc gtatgggtcc ttgccggcct cggtgaagcg
     2041 acgggtgatc cgcaggcctt tgctcatgaa acgtgcctcc tcgcacaggg tgcgccccgc
     2101 ccagtttacg gggcgatgtc cattgccaaa gcgtcgattc tcaactcaaa ccacaagatg
     2161 ttgtggtcga gggggaagtg accacgacat cttgtatgga caccactact gtaattacaa
     2221 cgatgtcatg ggtcaagtgt ttcgttatcc acaggccggg agcccgccga gaacgcgaaa
     2281 atcaacgttc tgggagggga agtgaacgtc ccggacctgc gctcggggaa gcaacggcac
     2341 cctaggccgg tggcctgccc gagggttggc tacgtcgctt cagagatttc gcgaccgccc
     2401 cggagatttc gccaatgcag ccatccagcg gtaacgatca gggggacgtg gagcaaaggg
     2461 aggctccggg ccagccagcc agggaggtag atgtctgtca ccgacccgtc ctcggcgccc
     2521 aacgactcgg cgagacccgt caagggacac cgggccccgt tggcaaggaa cacgagcgac
     2581 tcgacgccca cgatcgcggc cgccgctcca gcacgacgat ccgaccttcc ggcggcgccc
     2641 gacgccagca ggtagagcat cgccccctcc acgaacgccc agatgacggt gtggatgccc
     2701 ttgacgacgg cgagagcgcg atggtgctcg gacatgcgcc catcgtccgg ccgggacggg
     2761 ccttgctact agaggacttg gtccttcggt gagcccgtca caacccggcc tggtcccacc
     2821 aatatcggat catcagcgcg gcctcgggcc cggcatcgtc ctgccagtag tgaccggcgt
     2881 gacggaggac gtatccggtg cggttggtga ggatcgactc ccagcggcgc ttctccgtcc
     2941 cccggaaggc cggatcctga tccgcccaga acaggagtgt cggtttgtct gcgatctcac
     3001 cgagcctctc ctcgatcgag gcgaggaagg cagccgaccc ggtcagctct cgggggaaaa
     3061 cccggaccgg gagccttgac tcctgatcgg ggaacggccc gcgccacatc gccatctccc
     3121 gcccgctcgg tgcccggcgc cgcatcgccc ggcgcacgaa cacgttgacg aagacattgg
     3181 cccggtcgac caggaactca ccggtccgac cttcccccag ggctgaggcg aaacgctggg
     3241 tcgcgttgtc ggtgcggggc caggcccagg tgttgccca
//
LOCUS       DASTXO010000587         2228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_257377, whole genome shotgun sequence.
ACCESSION   DASTXO010000587 DASTXO010000000
VERSION     DASTXO010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257377"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VFU96_12490"
     CDS             complement(<1..643)
                     /locus_tag="VFU96_12490"
                     /inference="COORDINATES: protein motif:HMM:NF017709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU5114126.1"
                     /translation="MRKRLLIAAVALAALFATVLPVGARPLSSGTNQAADRAPYVVLM
                     AGEPVVAYEGGVPGLAGTAPPEGEKVDRNSAAVRAYRGHLHSQHVEAASAAGVSSSAI
                     LIDYEFALNGFTAVLTPAEAEKLAAQKNVISVVRDELRQLHTDTSPDFLGLTDGGGAW
                     DSGITGEGVVIGVIDTGIWPEHPSFADDGSYAAPPVSIEDVDVDPDPENEVILP"
     gene            806..1654
                     /locus_tag="VFU96_12495"
     CDS             806..1654
                     /locus_tag="VFU96_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEU5114127.1"
                     /translation="MNNLKTVGLLAFMTTLLWLVAYALFPEGGFVIGLLIAVGFNLLA
                     YFFSDKLALAASRARPVTEEELPQVYAIVRRLALQKNMPEPRIYIIDSPQPNAFATGR
                     SPKKAAVAVTAGILELLTVDELEGVLAHELAHVSNRDILISSIAAMIAAALTMLARMA
                     FWFGGGDNRESPLGAIGAIISLVVAPIAAMLIRLAISRTREFQADATGAETTGRPMML
                     ASALEKISAGTARIPMNVNPATAQLFIDNPMKAVRGSGLMKLLSTHPPTEERIERLTM
                     MAQGVR"
     gene            1738..1820
                     /locus_tag="VFU96_12500"
     tRNA            1738..1820
                     /locus_tag="VFU96_12500"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1772..1774,aa:Tyr,seq:gta)
     gene            1848..1920
                     /locus_tag="VFU96_12505"
     tRNA            1848..1920
                     /locus_tag="VFU96_12505"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1881..1883,aa:Thr,seq:ggt)
     gene            1951..2024
                     /locus_tag="VFU96_12510"
     tRNA            1951..2024
                     /locus_tag="VFU96_12510"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1985..1987,aa:Met,seq:cat)
BASE COUNT          416 a          706 c          713 g          393 t
ORIGIN      
        1 ctggaaggat gacctcgttc tctgggtcgg gatcgacgtc gacgtcctcg atggagaccg
       61 gtggtgccgc atacgagcca tcgtcggcaa aggatggatg ctcaggccag attccggtgt
      121 cgatcacacc gatgacaaca ccctcgcccg tgatgccgga gtcccacgcc ccaccgccgt
      181 cggtcagacc caagaaatcg ggtgaagtgt cggtgtgaag ttgccgcagc tcatcgcgga
      241 cgaccgagat gacgttcttt tgggcggcca gcttctcggc ttcggcggga gtgagcaccg
      301 cggtgaaccc gttcagagcg aactcgtagt cgatcaggat ggccgacgat gagacaccgg
      361 cggccgacgc ggcctcgacg tgctgactgt gcagatggcc ccgataggct cgcacggccg
      421 ccgagttgcg gtcgaccttc tcgccttctg gaggcgctgt gcctgccaga ccgggcacac
      481 caccctcgta cgcgacgact ggctctccgg ccatgagcac gacatatgga gctcggtcgg
      541 ccgcttgatt ggtgcccgat gagagtggac gggctcccac cggaaggacg gttgcgaaca
      601 atgccgccag ggcgactgcg gcaatgagaa gtctctttct caacttctct cctccttgac
      661 ttgttgcgcc tccctggcga acgagcccaa aggcaggacc ccacgatcag ggaccctaca
      721 ccactatcag tgggcgatgc caatggccga cccatacctg gaaagcgctc gcgtgtatcg
      781 ggtgggaggc cgtaaaatcc gagccatgaa caacctgaag acggtcggct tgctcgcctt
      841 catgaccacg ctcttgtggt tggttgccta tgccctcttc cccgaggggg gcttcgtcat
      901 cggtctcctg atcgcggtcg gcttcaacct gctcgcctac ttcttctcgg acaagctcgc
      961 cctggccgcc agccgggcac gtccggtgac cgaggaggag ctgccccagg tatacgccat
     1021 cgtcagacgt ctggcgctgc agaagaacat gccggagccg cgcatctaca tcatcgactc
     1081 gccccagccc aacgccttcg ccacgggccg ctcccccaag aaggccgccg ttgccgtcac
     1141 cgccgggatc ctcgagctgc tcaccgtcga cgagttggaa ggcgtgctgg cccacgagct
     1201 ggcccatgtg agcaaccgcg acatcctgat cagctcgatc gcggcgatga tcgctgccgc
     1261 cctgaccatg ctcgcccgga tggctttctg gttcggcggc ggcgacaacc gggagagccc
     1321 actcggcgcc ataggcgcca tcatctcgct ggtcgtggcc ccgatagcgg cgatgctgat
     1381 ccgcctcgcc atttcgcgaa cccgcgagtt ccaggcggat gccaccggtg ccgagactac
     1441 ggggcgcccg atgatgctgg ccagcgctct cgagaagatc tcagccggca ctgcccggat
     1501 accgatgaac gtgaacccgg ccaccgccca gctcttcatc gacaacccga tgaaggcggt
     1561 tcgcggcagc gggttgatga aactcctcag cacccacccg cccaccgagg agcgcatcga
     1621 gcggctcacg atgatggcgc agggagtccg gtagcgggca ggttggtaga gggccggagc
     1681 gccctgtaac attgcacatc ccgaaccgag cgttcttttg gcgtgcggtt ctggcatgcc
     1741 gggatgcccg agcggccaaa gggagcagac tgtaaatctg ccggctgacg cctacggagg
     1801 ttcgaatcct tctcccggca ccggcgcccg gcgcctgaca acccaacgcc cccttagctc
     1861 agttggtaga gcacttccat ggtaaggaag gggtcttcgg ttcaaatccg gaagggggct
     1921 ctgagcgagc gggtcaaccg cgcgcaccga ggcggcgtag ctcagttggt gagagcgctc
     1981 gactcataat cgagaggtcg gcagttcgag tctgcccgcc gctacatcca gggccgtggc
     2041 gtaggctgcg gcggccgaaa accgagaaaa ggaacaggag ccagacgatg gcgaaggcga
     2101 agtttgagcg tacgaagccg catgtgaatg tggggacgat gggtcatatt gatcatggga
     2161 agacgacgtt gacggctgcg attactcgtg ttttgtcgga gcgggtgggt ggttcgaatc
     2221 agatgacg
//
LOCUS       DASTXO010000588         2645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_1805182, whole genome shotgun sequence.
ACCESSION   DASTXO010000588 DASTXO010000000
VERSION     DASTXO010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1805182"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2599)
                     /locus_tag="VFU96_12515"
     CDS             complement(<1..2599)
                     /locus_tag="VFU96_12515"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEU5114128.1"
                     /translation="MRDASRLRELFGTRLHLEIWHHRMPEDDPRNDLMWEVAQKLSLP
                     AVATNQVHYHDPKDADLSEVLAAIGGRRTLAVADGFRPASDERYLKHPAEIARRFTRY
                     PGAVARSGELGRRLAFDLGLVAPNLPDFPMPGHFRDEMEYLRHLTWEGARDVYPGSED
                     GIDPRARRRLEHELDIIESLGFPGYFLVVKDIMDYARTQDIYCQIRGSGADSAVCRCL
                     GLTRVDPIRLHLPFERFLSEERGRPPDIDIDFEADRREEVIQYCYRTYGRERAAMVAN
                     VITYRARSVLRDVAKTFGFTQAQVDGLSKYVDTRDPAQIRLDSPLPEGMTAEMIYDIC
                     WRLDGFPRHLGIHSGGMVIADRPLWQVVPLEWGRLEGRTVLQWDKDDSAAVGIVKFDL
                     LGLGMLNALHLAHDLIEDTHGIDIDLAAIPQEQAIYRSITRSDTVGLFQIESRAQMAT
                     LPKMKPKTFYDLAVEVALIRPGPIQGQSVHPYLRRRNGEEPVVYPHPSTEEILGKTLG
                     VPVFQEQLMELARVCAGFDGGQADRLRQAMTHKRSEQAMGRLRDEVYEGMAGNGIVGP
                     AADEIWEKLQGFAAFGFPESHSVSFAYIVYMSAWLRFHFPAEYLAGLLNAQPMGFYSP
                     NSLVQDAQRHGVVVLAPDVNISWHDCTIEAIEADPDDVVSYLGESWRRGRGAIDDPIR
                     PPVGVRMGLRYVRNMGEKEVTRLEAGRILGGDFISGEDLAARTGLGVEALEGLAASGA
                     LESIGLGRREGMWAAGALAEIDPERLALSPGVDAPRLPTMAPEETHRADLWSTGVSNR
                     HPMSFVRDQMDGCLTAADALAMEKNGSRIKVAGVVTHRQRPGTAKGVYFLNLEDETGL
                     LNIIVLPDVW"
BASE COUNT          467 a          880 c          856 g          442 t
ORIGIN      
        1 cccagacatc gggcagcacg atgatgttga gcagcccggt ctcgtcttcc aggttgagga
       61 agtagacgcc tttggcggtg cccggtcgct gacgatgggt gacgacgccg gccaccttga
      121 tccgggaacc gtttttctcc attgccaggg catcggcagc ggtcagacat ccgtccatct
      181 ggtcgcggac aaagctcatc ggatgccgat tcgagacccc ggtcgaccac agatctgcac
      241 gatgcgtctc ctcgggagcc atcgtgggga gacgcggcgc atccacccca ggagacagcg
      301 ccagacgctc gggatcgatc tcggccagag cgcccgccgc ccacatcccc tcccggcggc
      361 cgagcccaat cgattccagt gcaccggagg ctgcgaggcc ctccaacgcc tccacgccga
      421 gcccggtccg cgccgccagg tcttcccccg agatgaaatc accaccgagg atccggccgg
      481 cctcgaggcg ggtcacttcc ttctccccca tgttccgcac atatcgcagc cccatccgga
      541 ccccgaccgg aggccgaatc gggtcatcga tcgcccccct cccccgccgc cagctctctc
      601 ccagatagct gacgacgtcg tcggggtcgg cttcgatcgc ctcgatcgtg cagtcgtgcc
      661 aggagatgtt gacgtcgggt gccagcacca ccacaccgtg ccgttgggcg tcctgaacga
      721 gtgagttggg gctgtagaag cccatgggct gggcgttgag gagccctgcc agatactcgg
      781 ccgggaaatg gaagcggagc caagccgaca tgtagacgat gtaggcgaag ctcaccgagt
      841 ggctctccgg gaaaccgaag gcagcgaagc cctgcaattt ctcccagatc tcatcggcgg
      901 cgggcccgac gatcccattg cccgccatac cttcgtagac ctcgtcccgg agtctcccca
      961 tcgcctgttc cgagcgtttg tgggtcatcg cctgtcggag gcggtcggcc tggcccccgt
     1021 cgaacccggc gcagacccgg gcaagctcca tgagctgctc ctggaagacg gggacaccca
     1081 gggtcttccc caggatctcc tcggtcgagg gatgggggta gacgaccggc tcctccccgt
     1141 tgcgccgccg caagtagggg tgcaccgact gcccctggat cgggccgggg cggatcaggg
     1201 ccacctcgac ggccaggtcg tagaaggtct tcggcttcat cttgggcaag gtcgccatct
     1261 gggcccggga ctcgatctgg aacaggccca cggtgtcgga gcgagtgatc gagcggtaga
     1321 tggcctgctc ttgaggaata gcggcaaggt cgatgtcgat gccgtgggtg tcctcgatca
     1381 ggtcgtgggc cagatgaagg gcgttgagca tccccagacc gagcagatcg aacttgacga
     1441 ttccaacggc cgccgagtcg tctttgtccc attggaggac agttcgcccc tcgaggcggc
     1501 cccactccaa cggaaccacc tgccagagag gccgatcggc aatcaccatc ccaccggagt
     1561 ggatcccgag atgccgggga aacccgtcga gccgccagca gatgtcatag atcatctcgg
     1621 ctgtcattcc ctcgggcagg gggctgtcga ggcggatctg ggccggatcg cgggtgtcga
     1681 cgtacttgga cagaccgtcg acctgggcct gggtgaatcc gaaggtcttg gcgacgtccc
     1741 ggagcaccga ccgagcccga taggtgatca cgttggccac catcgccgcc cgctcccgtc
     1801 cgtaagtgcg atagcagtac tggatcacct cctcccgacg atcggcctcg aagtcgatgt
     1861 cgatgtccgg tggccggccc cgctcctcgg agaggaagcg ctcgaaggga agatgaagcc
     1921 ggatcgggtc cacccgggtg agaccgagac agcggcacac ggcagagtcc gcccccgaac
     1981 cacggatctg gcagtagatg tcctgggtgc gggcgtaatc catgatgtcc ttcaccacca
     2041 ggaagtagcc ggggaacccg agggactcga tgatgtcgag ctcgtgctcg agccggcgcc
     2101 gtgccctggg gtcgatcccg tcttcgctcc cgggatagac atcccgtgct ccctcccagg
     2161 tgaggtggcg caaatactcc atctcatccc tgaagtgacc gggcatgggg aaatccggca
     2221 gattgggggc caccagcccc agatcgaagg caagtcgtcg accgagctcg ccgctcctcg
     2281 ccaccgctcc ggggtaccgg gtgaagcgcc gggcgatctc ggcagggtgc ttcaagtacc
     2341 tctcatcact tgccgggcgg aacccgtcgg cgacggccag cgtgcgccgg cctccgatgg
     2401 cagccagcac ttcggagaga tcggcgtctt tcgggtcgtg atagtggacc tggttcgtgg
     2461 ccacggcggg aagggacaac ttctgagcca cctcccacat caggtcgttg cgtgggtcgt
     2521 cttcgggcat gcggtgatgc cagatctcga gatggagccg ggtcccgaac agctcacgca
     2581 aacgggaggc atcccgcatg gcagcaccca gatctccact ggcggccgcg ttggggacgg
     2641 cgccg
//
LOCUS       DASTXO010000589         2532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U2417
            k141_184099, whole genome shotgun sequence.
ACCESSION   DASTXO010000589 DASTXO010000000
VERSION     DASTXO010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960876
            Sequence Read Archive: SRR25158461
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:46:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,481
            Genes (coding)                    :: 2,469
            CDSs (with protein)               :: 2,469
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 4 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184099"
                     /isolate="SMAG_U2417"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64111111 N 117.5011111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VFU96_12520"
     CDS             <1..449
                     /locus_tag="VFU96_12520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEU5114129.1"
                     /translation="GYGDARAVASSVLTAFATDHIDAVEVFFTRYQSAMNQVPTRLDL
                     LPITPPERAEKEGPSATYEFEPSPEEILDRLLPRYVEATVFNVLLEASASEHSARRRA
                     MKAATDNAEDLIRVLSVQANRARQAEITTAISEIVGGAEALAEAGR"
     gene            481..1908
                     /gene="atpD"
                     /locus_tag="VFU96_12525"
     CDS             481..1908
                     /gene="atpD"
                     /locus_tag="VFU96_12525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU5114130.1"
                     /translation="MSDAKRVGRIVKVAGPVVDVEFPPDGMPEILHAITIDFKLGDEK
                     RSVLAEVAQHLGGSKVRAIALAPTDGLTRGAEVVNTGGPITVPVGDQTLGHIFNVWGD
                     SLDAPDQDFSDPRWPIHRTPPAFEDVEPQKTVFETGIKVLDLISPYLQGGKIGLFGGA
                     GVGKTVLIQEMINRVATQHGGVSVFAGVGERTREGTDLFLEMKETGVIEKAALVFGQM
                     DEPPGVRLRVALSALTIAEYFRDVQRQDVLLFIDNIFRFTQAGSEVSTLLGRMPSEVG
                     YQPNLADEMGFLQERITSLKGRSITSMQAIYVPADDITDPAPHTAFAHLDATTVLSRP
                     LTALGIYPAVDPLDSSSRALDPQVVGTEHYFVATEVQRVLQRYKDLQDIIAILGIDEL
                     SEEDKLVVGRARRIQRFFAQPMFVAEQFTGQTGIYTPIEETIASFKAVLEGEMDQYPE
                     QAFYMVGGREDVERKAAELTKDAAA"
     gene            1908..2276
                     /locus_tag="VFU96_12530"
     CDS             1908..2276
                     /locus_tag="VFU96_12530"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEU5114131.1"
                     /translation="MANTFQVDVVSPEETVWSGTATFLLAKTPEGELGIMANHEPLMG
                     SLVTGAVIVEAEDGARTTIGVHGGFLQVLDNQVTLITDRAQITEGGREEARQVAEQLA
                     SEHEEEAEETLEAEPGPEPG"
     gene            complement(2277..>2532)
                     /locus_tag="VFU96_12535"
     CDS             complement(2277..>2532)
                     /locus_tag="VFU96_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5114132.1"
                     /translation="GRTYVGKATPAGRDEGHLMAWNIDSSEIKGKVSIELAPNAQGTN
                     VQVRLDVEGAGMLGSLFFPVIAAAIGSGFSTTVEEFVAGL"
BASE COUNT          475 a          843 c          801 g          413 t
ORIGIN      
        1 cgggatacgg tgacgcccgt gcagtggcca gctcggtgct caccgcattc gccaccgacc
       61 acatcgacgc ggtcgaggtg ttcttcacca ggtaccagtc ggcaatgaac caggtcccga
      121 cccgcctcga cctgctcccc atcaccccgc cggagcgagc cgagaaggag ggcccgtcgg
      181 ccacctacga attcgagccc tcacctgaag agatcctcga caggcttctc cccagatacg
      241 tcgaggcgac ggttttcaac gtgctgctcg aggcctcggc ttccgagcac agcgcccggc
      301 gccgggcaat gaaggcggcc acggacaacg ccgaggacct gatcagggtt ctgtccgtcc
      361 aggccaaccg ggcccgtcag gccgagatca cgacggccat ctccgagatc gtcggtggtg
      421 ccgaggcctt ggccgaggca ggccgatgaa ggattcacaa aaccaagaat ggagacagca
      481 atgagtgacg ccaagagggt cgggcggatc gtcaaggtcg ccgggccggt ggtcgatgtc
      541 gagttccccc cggatgggat gcccgagatc ctccatgcca tcaccatcga cttcaaactc
      601 ggtgacgaaa aacgctcggt cctggccgag gtcgcccagc atctcggtgg cagcaaggtg
      661 cgggccattg ccctggctcc caccgatggg ctgacccgcg gcgccgaggt cgtcaacacc
      721 ggcgggccga tcaccgtccc cgtcggcgat cagacgctgg gccacatctt caacgtctgg
      781 ggcgactcgc tcgatgcccc cgatcaggac ttctccgacc cgagatggcc gatccatcgc
      841 actccacctg ccttcgagga cgtcgagccc cagaagacgg tgttcgagac cgggatcaag
      901 gtcctcgacc tgatcagccc ctaccttcag ggcgggaaga tcggattgtt cggcggtgcg
      961 ggcgtcggca agacagtcct catccaggag atgatcaacc gggtcgccac ccagcacggt
     1021 ggtgtctcgg tgttcgccgg cgtgggggag cggacgcgtg agggcaccga tctcttcctc
     1081 gagatgaagg agaccggtgt catcgaaaag gccgccctcg tgttcggcca gatggacgag
     1141 ccccccgggg tgcgtctccg cgtcgccctg tccgccctga ccatcgccga atacttccgt
     1201 gacgtgcagc gtcaggacgt gctgctcttc atcgacaaca tcttccgctt cacccaggcc
     1261 gggtcggagg tctccacgtt gctcggcagg atgccctctg aagtggggta ccagcccaac
     1321 ctcgccgacg agatgggttt cctccaggag cggatcacct ctctgaaggg caggtcgatc
     1381 acgtcgatgc aggcgatcta tgtgcccgcc gacgacatca ccgacccggc acctcatacg
     1441 gcttttgccc acctcgacgc caccacggtg ttgtcccgtc ccctcaccgc cctgggcatc
     1501 tacccggccg tggacccgct cgattcctcg agccgcgccc tcgatcccca ggtggttggg
     1561 accgagcact acttcgtggc caccgaggtg cagcgcgtcc tgcagagata caaggacctc
     1621 caggacatca tcgccattct cggaatcgac gagctctccg aggaggacaa gctggtggtg
     1681 ggccgggccc ggaggatcca gcgtttcttc gcccagccga tgttcgttgc ggagcagttc
     1741 accggtcaaa ccgggatcta cacgccgatc gaggagacga ttgcctcgtt caaggccgtg
     1801 ctcgaaggcg agatggacca ataccccgag caagccttct acatggtcgg cggccgcgag
     1861 gatgtcgagc gcaaggcggc tgagctgacc aaggatgccg ccgcctagtg gccaacacgt
     1921 tccaggtcga cgtggtctcg cccgaggaga cggtgtggag cgggacagca acgttcctgc
     1981 tggccaagac gccggaaggc gagctgggaa tcatggccaa tcacgagccg ctgatgggat
     2041 ccctggtcac gggggcggtc atcgtggagg ccgaagatgg cgctcgcacc acgatcggtg
     2101 tccatggcgg gttcctccag gtgctcgaca accaggtgac cctcatcacc gaccgagccc
     2161 agatcaccga gggcggccgc gaagaggcga gacaagtggc ggaacagctc gcctcggagc
     2221 atgaggaaga ggcagaggaa actctcgaag ccgagcccgg gccagagccc ggatagtcac
     2281 agcccggcga cgaattcctc cactgtcgtc gagaatccgc tgccgattgc ggcggcgatg
     2341 accgggaaga agagggagcc gagcatcccg gctccttcga catcgagacg tacctggacg
     2401 tttgtgccct gggcattcgg ggccagctcg atcgacacct tgcccttgat ctccgaggaa
     2461 tcgatgttcc aggccatcag gtgaccctcg tcccggccgg ccggcgtcgc cttcccgaca
     2521 taggtccgtc cg
//
